LOCUS       DAZAFF010000001       102089 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000001 DAZAFF010000000
VERSION     DAZAFF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 102089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 102089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..102089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.001"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2169)
                     /locus_tag="ACG9BB_000001"
     CDS             complement(<1..2169)
                     /locus_tag="ACG9BB_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2835986.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFYKSLLFLPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFD
                     KESMIGDKVAKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDD
                     VPDDSEQINKKIFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMN
                     EFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLV
                     QELANYNEYTGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKD
                     NSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKW
                     EETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFIN
                     WFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTA
                     LKKIDCSSFAEERDFLSFILRCGVSKYPKIYDNDSLKNDVG"
     gene            complement(2292..3842)
                     /locus_tag="ACG9BB_000002"
     CDS             complement(2292..3842)
                     /locus_tag="ACG9BB_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2835987.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            complement(3844..4209)
                     /locus_tag="ACG9BB_000003"
     CDS             complement(3844..4209)
                     /locus_tag="ACG9BB_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2835988.1"
                     /translation="MDEQQAQQIREFFNIYLEGVGLQEAGKRSGINKPHPFMKRILMN
                     EVYLGNEIYPKIIDEDIFFQADLEQERRIKFFKKRKEIKVPDTKVIPTHFKSGKITEK
                     YSDPFKQAQYAYSKIKEVK"
     gene            complement(4257..5810)
                     /locus_tag="ACG9BB_000004"
     CDS             complement(4257..5810)
                     /locus_tag="ACG9BB_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2835989.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            complement(5871..6044)
                     /locus_tag="ACG9BB_000005"
     CDS             complement(5871..6044)
                     /locus_tag="ACG9BB_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFN2835990.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            6339..6644
                     /locus_tag="ACG9BB_000006"
     CDS             6339..6644
                     /locus_tag="ACG9BB_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2835991.1"
                     /translation="MSSFDMEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIE
                     AEEALYQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            complement(6705..7742)
                     /locus_tag="ACG9BB_000007"
     CDS             complement(6705..7742)
                     /locus_tag="ACG9BB_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFN2835992.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(7858..8766)
                     /locus_tag="ACG9BB_000008"
     CDS             complement(7858..8766)
                     /locus_tag="ACG9BB_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFN2835993.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            complement(8756..9175)
                     /locus_tag="ACG9BB_000009"
     CDS             complement(8756..9175)
                     /locus_tag="ACG9BB_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFN2835994.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            complement(9176..9583)
                     /locus_tag="ACG9BB_000010"
     CDS             complement(9176..9583)
                     /locus_tag="ACG9BB_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HFN2835995.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            complement(9570..9737)
                     /locus_tag="ACG9BB_000011"
     CDS             complement(9570..9737)
                     /locus_tag="ACG9BB_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2835996.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            complement(9747..13637)
                     /locus_tag="ACG9BB_000012"
     CDS             complement(9747..13637)
                     /locus_tag="ACG9BB_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN2835997.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            complement(13634..14326)
                     /locus_tag="ACG9BB_000013"
     CDS             complement(13634..14326)
                     /locus_tag="ACG9BB_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN2835998.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            complement(14403..14804)
                     /locus_tag="ACG9BB_000014"
     CDS             complement(14403..14804)
                     /locus_tag="ACG9BB_000014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2835999.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            complement(14875..16935)
                     /locus_tag="ACG9BB_000015"
     CDS             complement(14875..16935)
                     /locus_tag="ACG9BB_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN2836000.1"
                     /translation="MGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTV
                     QKNADNAFKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKM
                     GTDIESIQNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFAD
                     VTEAIHVIQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNV
                     VEQFQNVIKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGL
                     LPTLIPVVVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIIT
                     IVNGLIENLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQ
                     TGIQLLTSLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALP
                     QIITTIANAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQII
                     TGLANAFGEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIH
                     SPSTLFRDEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDS
                     TFSMLDLGAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRR
                     RVAY"
     gene            complement(17455..17595)
                     /locus_tag="ACG9BB_000016"
     CDS             complement(17455..17595)
                     /locus_tag="ACG9BB_000016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836001.1"
                     /translation="MGMTSDDFWYCSLGLFLDLWECHKQFTGISKPKVELFIDDVIPS
                     GI"
     gene            complement(17643..18023)
                     /locus_tag="ACG9BB_000017"
     CDS             complement(17643..18023)
                     /locus_tag="ACG9BB_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836002.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            complement(18033..18623)
                     /locus_tag="ACG9BB_000018"
     CDS             complement(18033..18623)
                     /locus_tag="ACG9BB_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFN2836003.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            complement(18626..18967)
                     /locus_tag="ACG9BB_000019"
     CDS             complement(18626..18967)
                     /locus_tag="ACG9BB_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836004.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            complement(18964..19395)
                     /locus_tag="ACG9BB_000020"
     CDS             complement(18964..19395)
                     /locus_tag="ACG9BB_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFN2836005.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            complement(19388..19726)
                     /locus_tag="ACG9BB_000021"
     CDS             complement(19388..19726)
                     /locus_tag="ACG9BB_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN2836006.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            complement(19723..20034)
                     /locus_tag="ACG9BB_000022"
     CDS             complement(19723..20034)
                     /locus_tag="ACG9BB_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN2836007.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            complement(20045..21256)
                     /locus_tag="ACG9BB_000023"
     CDS             complement(20045..21256)
                     /locus_tag="ACG9BB_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN2836008.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            complement(21260..22015)
                     /locus_tag="ACG9BB_000024"
     CDS             complement(21260..22015)
                     /locus_tag="ACG9BB_000024"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFN2836009.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            complement(21915..23243)
                     /locus_tag="ACG9BB_000025"
     CDS             complement(21915..23243)
                     /locus_tag="ACG9BB_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN2836010.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            complement(23309..24043)
                     /locus_tag="ACG9BB_000026"
     CDS             complement(23309..24043)
                     /locus_tag="ACG9BB_000026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836011.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSLTPDEKS
                     KCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            complement(24099..25664)
                     /locus_tag="ACG9BB_000027"
     CDS             complement(24099..25664)
                     /locus_tag="ACG9BB_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN2836012.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            complement(25747..25890)
                     /locus_tag="ACG9BB_000028"
     CDS             complement(25747..25890)
                     /locus_tag="ACG9BB_000028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836013.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            complement(25887..26063)
                     /locus_tag="ACG9BB_000029"
     CDS             complement(25887..26063)
                     /locus_tag="ACG9BB_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HFN2836014.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            complement(26047..26241)
                     /locus_tag="ACG9BB_000030"
     CDS             complement(26047..26241)
                     /locus_tag="ACG9BB_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836015.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            complement(26243..26620)
                     /locus_tag="ACG9BB_000031"
     CDS             complement(26243..26620)
                     /locus_tag="ACG9BB_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HFN2836016.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            complement(26694..27929)
                     /locus_tag="ACG9BB_000032"
     CDS             complement(26694..27929)
                     /locus_tag="ACG9BB_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFN2836017.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            complement(27929..28711)
                     /locus_tag="ACG9BB_000033"
     CDS             complement(27929..28711)
                     /locus_tag="ACG9BB_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HFN2836018.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            complement(28726..29298)
                     /locus_tag="ACG9BB_000034"
     CDS             complement(28726..29298)
                     /locus_tag="ACG9BB_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFN2836019.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            complement(29432..29797)
                     /locus_tag="ACG9BB_000035"
     CDS             complement(29432..29797)
                     /locus_tag="ACG9BB_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN2836020.1"
                     /translation="MPMKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAIN
                     KRYGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCK
                     SCHSKIHMTRRNTKDKTIM"
     gene            complement(29965..30393)
                     /locus_tag="ACG9BB_000036"
     CDS             complement(29965..30393)
                     /locus_tag="ACG9BB_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFN2836021.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            complement(30390..31742)
                     /locus_tag="ACG9BB_000037"
     CDS             complement(30390..31742)
                     /locus_tag="ACG9BB_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFN2836022.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            complement(31742..32026)
                     /locus_tag="ACG9BB_000038"
     CDS             complement(31742..32026)
                     /locus_tag="ACG9BB_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFN2836023.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            complement(32023..32232)
                     /locus_tag="ACG9BB_000039"
     CDS             complement(32023..32232)
                     /locus_tag="ACG9BB_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836024.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            complement(32370..34613)
                     /locus_tag="ACG9BB_000040"
     CDS             complement(32370..34613)
                     /locus_tag="ACG9BB_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFN2836025.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            complement(34606..34950)
                     /locus_tag="ACG9BB_000041"
     CDS             complement(34606..34950)
                     /locus_tag="ACG9BB_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFN2836026.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            complement(34943..35713)
                     /locus_tag="ACG9BB_000042"
     CDS             complement(34943..35713)
                     /locus_tag="ACG9BB_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFN2836027.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            complement(35924..37861)
                     /locus_tag="ACG9BB_000043"
     CDS             complement(35924..37861)
                     /locus_tag="ACG9BB_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFN2836028.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     ATHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            complement(37922..38464)
                     /locus_tag="ACG9BB_000044"
     CDS             complement(37922..38464)
                     /locus_tag="ACG9BB_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFN2836029.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            complement(38466..39608)
                     /locus_tag="ACG9BB_000045"
     CDS             complement(38466..39608)
                     /locus_tag="ACG9BB_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFN2836030.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            complement(39609..39923)
                     /locus_tag="ACG9BB_000046"
     CDS             complement(39609..39923)
                     /locus_tag="ACG9BB_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HFN2836031.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            complement(39928..40134)
                     /locus_tag="ACG9BB_000047"
     CDS             complement(39928..40134)
                     /locus_tag="ACG9BB_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836032.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            complement(40231..40848)
                     /locus_tag="ACG9BB_000048"
     CDS             complement(40231..40848)
                     /locus_tag="ACG9BB_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836033.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            41243..42193
                     /locus_tag="ACG9BB_000049"
     CDS             41243..42193
                     /locus_tag="ACG9BB_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFN2836034.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            42186..42632
                     /locus_tag="ACG9BB_000050"
     CDS             42186..42632
                     /locus_tag="ACG9BB_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFN2836035.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            42635..44467
                     /locus_tag="ACG9BB_000051"
     CDS             42635..44467
                     /locus_tag="ACG9BB_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2836036.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            44483..44686
                     /locus_tag="ACG9BB_000052"
     CDS             44483..44686
                     /locus_tag="ACG9BB_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2836037.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(44737..45873)
                     /locus_tag="ACG9BB_000053"
     CDS             complement(44737..45873)
                     /locus_tag="ACG9BB_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN2836038.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(45863..47818)
                     /locus_tag="ACG9BB_000054"
     CDS             complement(45863..47818)
                     /locus_tag="ACG9BB_000054"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN2836039.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            complement(47811..50528)
                     /locus_tag="ACG9BB_000055"
     CDS             complement(47811..50528)
                     /locus_tag="ACG9BB_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFN2836040.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            complement(50529..50750)
                     /locus_tag="ACG9BB_000056"
     CDS             complement(50529..50750)
                     /locus_tag="ACG9BB_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2836041.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            complement(50899..51735)
                     /locus_tag="ACG9BB_000057"
     CDS             complement(50899..51735)
                     /locus_tag="ACG9BB_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN2836042.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            complement(51944..53320)
                     /gene="rlmD"
                     /locus_tag="ACG9BB_000058"
     CDS             complement(51944..53320)
                     /gene="rlmD"
                     /locus_tag="ACG9BB_000058"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFN2836043.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            complement(53431..54486)
                     /locus_tag="ACG9BB_000059"
     CDS             complement(53431..54486)
                     /locus_tag="ACG9BB_000059"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFN2836044.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(54496..55926)
                     /gene="gatB"
                     /locus_tag="ACG9BB_000060"
     CDS             complement(54496..55926)
                     /gene="gatB"
                     /locus_tag="ACG9BB_000060"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFN2836045.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(55923..57395)
                     /gene="gatA"
                     /locus_tag="ACG9BB_000061"
     CDS             complement(55923..57395)
                     /gene="gatA"
                     /locus_tag="ACG9BB_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFN2836046.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(57395..57700)
                     /gene="gatC"
                     /locus_tag="ACG9BB_000062"
     CDS             complement(57395..57700)
                     /gene="gatC"
                     /locus_tag="ACG9BB_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFN2836047.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(57806..59842)
                     /gene="ligA"
                     /locus_tag="ACG9BB_000063"
     CDS             complement(57806..59842)
                     /gene="ligA"
                     /locus_tag="ACG9BB_000063"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFN2836048.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(59859..62093)
                     /gene="pcrA"
                     /locus_tag="ACG9BB_000064"
     CDS             complement(59859..62093)
                     /gene="pcrA"
                     /locus_tag="ACG9BB_000064"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFN2836049.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            62248..63807
                     /locus_tag="ACG9BB_000065"
     CDS             62248..63807
                     /locus_tag="ACG9BB_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFN2836050.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(63918..65849)
                     /locus_tag="ACG9BB_000066"
     CDS             complement(63918..65849)
                     /locus_tag="ACG9BB_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFN2836051.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(65867..66781)
                     /gene="pfkB"
                     /locus_tag="ACG9BB_000067"
     CDS             complement(65867..66781)
                     /gene="pfkB"
                     /locus_tag="ACG9BB_000067"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFN2836052.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(66778..67530)
                     /locus_tag="ACG9BB_000068"
     CDS             complement(66778..67530)
                     /locus_tag="ACG9BB_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN2836053.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(67689..67898)
                     /locus_tag="ACG9BB_000069"
     CDS             complement(67689..67898)
                     /locus_tag="ACG9BB_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836054.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(67909..69237)
                     /locus_tag="ACG9BB_000070"
     CDS             complement(67909..69237)
                     /locus_tag="ACG9BB_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836055.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            69373..69528
                     /locus_tag="ACG9BB_000071"
     CDS             69373..69528
                     /locus_tag="ACG9BB_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFN2836056.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(69602..70189)
                     /gene="yihA"
                     /locus_tag="ACG9BB_000072"
     CDS             complement(69602..70189)
                     /gene="yihA"
                     /locus_tag="ACG9BB_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFN2836057.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(70272..71522)
                     /gene="clpX"
                     /locus_tag="ACG9BB_000073"
     CDS             complement(70272..71522)
                     /gene="clpX"
                     /locus_tag="ACG9BB_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFN2836058.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(71769..73058)
                     /gene="tig"
                     /locus_tag="ACG9BB_000074"
     CDS             complement(71769..73058)
                     /gene="tig"
                     /locus_tag="ACG9BB_000074"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFN2836059.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(73212..74144)
                     /locus_tag="ACG9BB_000075"
     CDS             complement(73212..74144)
                     /locus_tag="ACG9BB_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836060.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(74301..74960)
                     /locus_tag="ACG9BB_000076"
     CDS             complement(74301..74960)
                     /locus_tag="ACG9BB_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFN2836061.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(74963..75988)
                     /gene="sppA"
                     /locus_tag="ACG9BB_000077"
     CDS             complement(74963..75988)
                     /gene="sppA"
                     /locus_tag="ACG9BB_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFN2836062.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(76001..76291)
                     /locus_tag="ACG9BB_000078"
     CDS             complement(76001..76291)
                     /locus_tag="ACG9BB_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836063.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            76701..78506
                     /gene="glmS"
                     /locus_tag="ACG9BB_000079"
     CDS             76701..78506
                     /gene="glmS"
                     /locus_tag="ACG9BB_000079"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFN2836064.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(78578..79933)
                     /gene="glmM"
                     /locus_tag="ACG9BB_000080"
     CDS             complement(78578..79933)
                     /gene="glmM"
                     /locus_tag="ACG9BB_000080"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFN2836065.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(79957..81126)
                     /locus_tag="ACG9BB_000081"
     CDS             complement(79957..81126)
                     /locus_tag="ACG9BB_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFN2836066.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(81123..82010)
                     /gene="cdaA"
                     /locus_tag="ACG9BB_000082"
     CDS             complement(81123..82010)
                     /gene="cdaA"
                     /locus_tag="ACG9BB_000082"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFN2836067.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(82199..85879)
                     /gene="nifJ"
                     /locus_tag="ACG9BB_000083"
     CDS             complement(82199..85879)
                     /gene="nifJ"
                     /locus_tag="ACG9BB_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFN2836068.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            86077..86598
                     /locus_tag="ACG9BB_000084"
     CDS             86077..86598
                     /locus_tag="ACG9BB_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFN2836069.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(87297..87862)
                     /locus_tag="ACG9BB_000085"
                     /pseudo
     CDS             complement(87297..87862)
                     /locus_tag="ACG9BB_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(88041..88697)
                     /locus_tag="ACG9BB_000086"
     CDS             complement(88041..88697)
                     /locus_tag="ACG9BB_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836070.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(89265..90200)
                     /gene="whiA"
                     /locus_tag="ACG9BB_000087"
     CDS             complement(89265..90200)
                     /gene="whiA"
                     /locus_tag="ACG9BB_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFN2836071.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(90234..91232)
                     /gene="yvcK"
                     /locus_tag="ACG9BB_000088"
     CDS             complement(90234..91232)
                     /gene="yvcK"
                     /locus_tag="ACG9BB_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFN2836072.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(91229..92113)
                     /gene="rapZ"
                     /locus_tag="ACG9BB_000089"
     CDS             complement(91229..92113)
                     /gene="rapZ"
                     /locus_tag="ACG9BB_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFN2836073.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(92248..92979)
                     /locus_tag="ACG9BB_000090"
     CDS             complement(92248..92979)
                     /locus_tag="ACG9BB_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFN2836074.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(92983..94248)
                     /locus_tag="ACG9BB_000091"
     CDS             complement(92983..94248)
                     /locus_tag="ACG9BB_000091"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFN2836075.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(94511..97327)
                     /gene="uvrA"
                     /locus_tag="ACG9BB_000092"
     CDS             complement(94511..97327)
                     /gene="uvrA"
                     /locus_tag="ACG9BB_000092"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFN2836076.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(97337..99331)
                     /gene="uvrB"
                     /locus_tag="ACG9BB_000093"
     CDS             complement(97337..99331)
                     /gene="uvrB"
                     /locus_tag="ACG9BB_000093"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFN2836077.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            99590..101092
                     /locus_tag="ACG9BB_000094"
     CDS             99590..101092
                     /locus_tag="ACG9BB_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFN2836078.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            101094..101831
                     /locus_tag="ACG9BB_000095"
     CDS             101094..101831
                     /locus_tag="ACG9BB_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN2836079.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
BASE COUNT        28241 a        22722 c        18133 g        32993 t
ORIGIN      
        1 tccaacatca ttttttaaag aatcgttatc ataaatctta ggatatttgg aaacaccaca
       61 tcgaagaata aatgacagaa aatctctctc ctcagcaaaa ctagagcaat ctattttttt
      121 caatgccgtg taatctgtat cttgaaaaag cttatctcct aattcattgc catactcttt
      181 tccaatatac attttattgg aaggttgaac gtcaccagca acgtttggaa gattaaatgg
      241 aactttagac agctcacttc cagctgatag tttttcattg tagttatcat aaaaccaatt
      301 aatgaaactg atagattttt caacagtgtc aatttgtcga ttaatcgttc caatgatcaa
      361 atctgaactg ctttgctcag tgaattcaat tgcaagatat ttttcactga atgcatctag
      421 aatacgttta tctcctgtac gttcagcttt gtatgcatta gctgtttctt cccatttatt
      481 tttccaactt gatgaagtct taatctgata aattaactca tcaacatatg tttgtttcaa
      541 aatgcttaaa ctaacccaag aagacagctc ttttggaagc acagtaacac ctgtatcggt
      601 cggtaagaat accttatctg atttcatcaa ccaattgtca ctattgtcct ttaatagctt
      661 agggacaatc ggagaacttt tatattgctc actccaccat aagaacactt ttgtccttat
      721 tctattttcc caagaagatg aaagaatgtt gattttatca gatagttcat ctacaacaaa
      781 cgacaggcca gtgtattcat tataattagc aagttcttgt actagttggt cattttcgtt
      841 tgtttctaat aattgcaaca aggtggaaaa tgtctcacct attagctcct cagggaattc
      901 ttgaaggaaa agttttggat tatctttgat agaaatatat tgccaattga cagtcggtag
      961 tatttgggca ttagataaaa tacccaagaa ttcgttcatt aagtcaaata catctaagtc
     1021 ccatagttta ttctctttaa aaggagttac catttttaat gctatgtctt catattcagg
     1081 cttagataat ttttctgcta cttcagaaat aaatttgagc acctcaataa atattttctt
     1141 atttatttgt tcactatcat ccgggacgtc atcacgtgaa ttatttgtta taaacggtgc
     1201 atttataagt gcatgaattg gtgtaccaaa attacgtact ggaaagtagc aatatagttt
     1261 ttctttggag taatcgtaat cagaatcttt agaaacggct agtgcaattt tagcaacttt
     1321 atctcctatc atagattctt tgtcaaaaac aaaatattct tcagacggtg attgaccagt
     1381 aacatcactt ttttctatca atacatcatt tccatccaca attttctctg atattttttc
     1441 atcatcagtt gtttcaataa caatttttgt tatatttggt aaaaataaca gtgacttata
     1501 aaatggctgc tgaagttgtt tagaaacact cgtgccttta cttaaattag tttcttttag
     1561 ttttactcga attaatgtat caaaaccaga atcaattttg tttattggtt gaggacagtt
     1621 cattattgga aaacataaat cataatcttc ttttttccaa tttttcttct gctcaaaaat
     1681 taattcactg aatttgtcag aatctcttga tacatactgc tcaaaaagct tttttgtgaa
     1741 ttcttcacta aatcgaatag caaattctcc cgaatacagt tcgacccact cagcatcgtt
     1801 tagcaaggaa cgaaaaccag tccccttatt tccgatattt tgtgatgatt tattactcgc
     1861 tcttcctaag cacaatcttt caattgtatc ttttgaaaat gaagtgcctg tattcccaac
     1921 ttccaagata ttatttttat atgttatctg aactttcact tcgtcattgc tagcatcatt
     1981 ttctgatttt atatctccat aggcatcgtc tacattctgt aataactcta aaatctgacg
     2041 cccattataa tcacgcatca attcatattc ttcgctgcag gccttttcaa gttcattact
     2101 caactcagaa tccgtatatt tacgaataaa aatatccctt aattcatcaa tggtattcca
     2161 ttggctcaca gaacttcgcc cccagcctat tttttacata atatattata tcatcaatca
     2221 ctttatgaaa tccaatcaat aaagacgtta atatggctac ccacattaac gtctagtcct
     2281 atctattcaa attaaatttc tacagtcact ctcaccccac ttttgaaatc aacaacaatg
     2341 ttcttttcaa aaatggtgac cttctcaatc aaccttcgaa caagcacttc attatactca
     2401 ttgatgacat cgtgttcttt tacaaagact accatctcat caacccgttg ttgcaaatta
     2461 gtattcgctg ctatttgttt tgcaaccgca tctcgttcat ctcttagtga aaatatttgg
     2521 cttgccaatt catcgtaacc ctgaccgcca actgccaggc taataatttc agcttcaagc
     2581 tctttcaact gactgtctag ttctgccaat cggtcagtta atccatcagt cataactgag
     2641 ttgatattgt catttaattg ctgaagaaaa gcatcacgat cgattaacgt ttcattgatt
     2701 gccttgataa cagctccatg caggtctgtt tcgtagattg ttcgacccgt gcaattatgt
     2761 tctcgtttat ccactctgga tttgcatcgc cacacagttc ctcgattctt ccaatgaact
     2821 ctgcggtaag cattgccaca gtcaccgcaa actgtgatac cagataacgc atacttccca
     2881 gaaaagcact gtgacttttt gtttttcatt gatttacgcc ggtctagttc ttgctgggct
     2941 tgcatgaaga tatcttttgg gataatggcc ggatggctat cttcaacata atactgctga
     3001 gcgtaactcc cgtcattctt cactcgtttc tttgtcaaga aatcggtagt atatgttttt
     3061 tgaagcagcg catcccccat gtacttctcg tttcgaagaa tcaatctgac tccttctcct
     3121 cgccagagct ttcgcttccc tcccgttagg atgccatcag ccattagacc attagcaatt
     3181 tcttgcaagg aactgccctc aaggtattcg cggaagatgc gtttgatgac ttctgcttcc
     3241 tttggttcaa tcaccaagtt accatcttcg tccttagtgt acccaagaaa atgattgtgg
     3301 ttgatacgta gctgtccctg ctggaaacga tactgtacgc ccatcttggt gttttgactt
     3361 agtgactggc tttcttgctg ggccagactt gccataatgg tcaaaagtac ctcacccttc
     3421 gcatccattg tattgatgtt ttccttttca aaaaacactg caatcttcag ttcttttaac
     3481 tgtcgtatgt attgcaagca atcaaccgta ttacgggcaa agcgtgaaat tgattttgtg
     3541 atgatgtact caacttcgcc gttttgacac gcctcaatca tccggttaaa accgtcacgc
     3601 tttttcgtcc cagttcctga aatcccttca tccgcaaata ttcctacaag ctcccaatcg
     3661 ggatttcttt gaatgtagtc cgtatagtgt tgaacctgtg cttcgtaaga actcgcctgt
     3721 tcctccattt cagtagaaac acgacaatag gctgccactt tcaactttgg tttctcttcg
     3781 gtattctctg ctgcccattg tctaggcttc attggaataa ccgtcacctg ttttcctgcc
     3841 atttcactta acctccttga tttttgaata tgcatattgc gcttgtttga atgggtcaga
     3901 atacttttcg gtaatcttgc cagatttaaa atgagtgggt attaccttcg tatctggcac
     3961 tttgatctcc ttgcgttttt taaagaactt aattcttcgt tcttgctcca gatccgcttg
     4021 gaagaaaata tcctcatcaa tgatttttgg atatatctca tttcctaagt aaacctcatt
     4081 catcaaaata cgtttcataa agggatgcgg cttgttaatg ccagagcgtt tgccagcttc
     4141 ttgtaagcca acaccctcaa ggtaaatatt gaagaactca cgtatttgtt gagcttgctg
     4201 ttcatccacg ttgatttctc cattcttaat gacatacccg tatggaattg tattgttcat
     4261 tctggtaacc tctctttcaa aagtagtccg cacttaagtt caaatccgac ttcttggcgg
     4321 ttgtaaactg taatgctgtt tgtgaacttc ttgaataaat cttcatccca gtccgtcaac
     4381 atctctgcca tccgcgcgaa tttgtataag gcttctagtt caatttgctg agtgcttttg
     4441 ccgttgactg actcaatgat gaggcgctgc ttttcttgtg cgacagccct ctcttgttga
     4501 agtttgcttt gttcttctaa agacaccttc gttgataaaa tactggaagc agtcaaactt
     4561 gccagaacct caagcttttc ttgaatcttg tcgatttcat cagcaagcac ttcgagccct
     4621 tcctgattcg cacctgcttt attcaaagac ttcataaaag gttccagaac caacttgtgt
     4681 ccgaaaatca acttattcaa catggtcaaa taagcaacct tcaaatcttc atccgtaatt
     4741 ggtaatgcat cgcagctatc tttattttta agatgccccg tacacttcca ctgaaccttc
     4801 ttcgtattac ctttccaata agtgtgaacc cgcttgaatt tgctgccgca tttatcacag
     4861 atgatgttgc ctgaaaatgc gtatcgcttg gtgtactttt tcccgtcacc agtattccct
     4921 ttggcctcag cttttctccg aatgagttct tgaactcggt cgtaatcttc tttagatact
     4981 atgggttcgt gattgttttt gatctggtac attggcaatt cgcccttgtt tatgcgtcgt
     5041 ttgtactggt catcaacaaa ggtttgttgc atcgtcaaag tcccggtata aacttcattt
     5101 ttcaaaatac ttctgactga tgttgcttgc caatttgccc cacgtttagt gaattgtttt
     5161 ccatcgttta accgcttggc aatcattcca gtcgaaatgc catctaatgc ccactggaac
     5221 atttgtttaa caacttttgc ttcagctgca acgatgtgaa aatcatagcc tttccgcttg
     5281 tagccatacg gcgggtaagc tttgaatttc cctttagaca tccggtttcg aatcgcccac
     5341 ttcaagtttt ctgaatggga ataggattca ctttctgcca agcttgataa aattgaaagc
     5401 aacaactcgc tttccatatc accagtatta atgttttcct tttcaaaaaa gatggtgaca
     5461 ccaatatcag ttagctgccg aaccagattc aagcagtcaa ctgtatttct tgaaaatctg
     5521 gaaatagact ttgtcagaat caaatcaatc tttccaagct ctgcatcctt aatcaatcgt
     5581 tttaaaccat cacgttttac agtcttggtt ccagaaatcc cttcatcata ataaacaccg
     5641 acatagtccc attgttcatg gttcttgatg tgattttcat agtgagtaat ctgggtttgt
     5701 aaacttgcca gctgttcatt acttgatgtt gatacacgag cgtatgctgc cacctttagt
     5761 ttggaagctt ccggcaactc tggacgtgcc aatttatgta tcgttttcat cagctcaact
     5821 cctttccatt aggtgacatg ttagctctaa agtgccgtta tatcaacgtt tcagaccatt
     5881 atccttgata aaaaaggatt gaatgtttcc cggtttttat tgtcgatttt gacgtattct
     5941 tccaggttga ttttaccggt aacaagtaag ttcagaagga tctgctgtga aatgtaatag
     6001 gctgcttcat ctgaatagtt aatttctgtt ttcttgttag tcattgcttt cgcctcctca
     6061 agatacaggc gaagaaaatc catgaaagta aacccaatca caaattttcc tttgcctttc
     6121 atccatatgc gaaagaaata accgactcga acccacaaaa cgaaaaaagc ctgcaagaat
     6181 gaaacgaatc actcttgcag gcttctaagt ttgtttatat atagtaggaa ttattctttg
     6241 taaattttga ctttttactt cccaaacatc aaataagcct ttacaagctt catccaataa
     6301 ataacaatat aaatatcaat tgtgggaagg agaaagttat gagtagtttt gatatggaag
     6361 ggttagaaaa agaatatgaa catttttgca aatctgcgac aacaaaggaa ctggaggaat
     6421 ttgatgaaag cttcaaacaa gaatttgaaa agtgctttaa aaccatagag gctgaagaag
     6481 ctttatacca gtttttacaa gccaatataa acgcgatgaa taatcgtatg gaatcaatag
     6541 aatcgcctct tttctgcatc aattacaaag agcaggtatt tcagttaaga aatcatttaa
     6601 tcctaatgca aaatgctgta aacacctacc ttaaagaaaa ctagacgaaa aaaaggcccc
     6661 acaataaaac ttgtgaggct gttttgaatt ataaagacgt caaactactt attgaaaatt
     6721 ttgtagctcg ttggaaccat cgtgaagttt tcttcagtaa ttgggccatc ataaattttg
     6781 attttgcaaa cttttgacgc cacatcaagg tcgtgaacaa agtaaatttt atatggtgta
     6841 tctccaaggg aggcagcaac ctcattagta ctcatttcaa atccgtcaga gaagtttgtt
     6901 tttgaagctt tcacttcaat atggatttca ttacctgatt ggtcaaacgc acaaatatca
     6961 tagccatgac catctccttc agttttagaa acatgttcag gaagttttgt tttgttcttt
     7021 tctgcttcct gaatgaggaa atcaagtacg atttcttcgc ctagagcacc cgttttttgt
     7081 tttgccttgt tgacacgctc ccaatcagta tggcgtccag tgaattttcg tttctgagtc
     7141 ccattcgtgc gatttggtcc cacttcatca acaagaacag taaatccttt tgaactttct
     7201 tgctcaattt cttcaacttc ctcgtttgta agatccagtt cagacggcaa cgattgtgta
     7261 tcatcgattg ggataagatg acgattttca atcaatgcaa caaaatcggc ataggtaatt
     7321 tcattcattc catactgatc ccagaagtag gcccatgaat tcggcttttt acgcttcttt
     7381 ttgctaggtg tattccaatc gaagttttca ataaatgaat caccttgttt aattgccggt
     7441 tcgattgtaa atgcgtgtgt aattactgcc gtaacatttc cagcttcatc aggttcagaa
     7501 atgctctcaa cgtatccgcc cccgtaaatt tcccacttac ggtctttagt aattttcccc
     7561 ggatgacgat tcaaaacaac cgcgccggct ttcaatttac catattggcg catgttccaa
     7621 ccgtaacttt tagcatcatc agcgtatttc aactgaccat catcttctgt tttcccgtga
     7681 tctaaaatga aagcttgttg gctttctgtt ggttctggta acaaatcttt aaacgtaacc
     7741 atatcgatac ctctttctat aattcaatcg gaacaaattt atgttctggt tgaattaata
     7801 ataacacgtt cgatatttta agtcaacaat ccggacataa ttttgttccg attaaattta
     7861 tgaaatcaaa atataatcgt cagattccgt cttaccagta ttcttaccga cagcacaata
     7921 tccaatcggg caacgagccc atacccaacc atccgcaatc gtcatcttct ctgacttcgg
     7981 aagcgtccaa gttgcgcctt tattgtatga accaacaacc gcaccactta aacttggctt
     8041 gttgcgaacg ttgagaccac tcacttgaac cacaagagtt ttcacgttgg atgttgatga
     8101 cccagatgga taaacttgat taccgttctg gtcgaacacc ttataaccag aatggctatc
     8161 tgcagcccgc ttcgcattgt tcaagtcctt ataggcacca atttgtgact tggcgtcagc
     8221 ccatgatttg cgaacacgat acagaccact agctggcgtg cttggggttg atgaaccatt
     8281 attaatcgca ttggctttat ccgcaatctc ctgcagttta ccaagcaact gtcccggaca
     8341 tgatgtggcc gaaaaatgtg agtgagggaa gatattcttg ttcaccacca atttccctag
     8401 accataacgt ttggcaatat ctgcacacaa ctttgcactt gcatcaatag ttgcttgatt
     8461 gataccccag ttcggtgatc cagtgctatt gcagtgctca atcccgatac ttgagatatt
     8521 ggcgttccaa ttaccagcgt gccaagctgt gtttccttcg tccacgtatg ctcgaatttc
     8581 tccatttttt ccaataccgt agtgtgctga cgcctcacgc gttttccaaa tatcaggaac
     8641 aatatcatag ttggtggttg ccatgtgatg aatcactatc ttatctactt tggcaccact
     8701 acgaccattc gagtaatggc taaatgactg aaatccatat tttgttgcta gattactaat
     8761 tcccattttc tgtttcctcc ttcttttcgt gtaactgctg caagatttca cgcaactttt
     8821 ccggcactgg cagtccaatc gccacggcat tctccagcag tgaaattcct tcgtttgaga
     8881 tatagaagaa aatgaccgca tcacgcagta catttccacc tttgatgact tccacatcaa
     8941 gcatatgagc cacaccaacc aaaagaaaaa tcatgatttt cttagtgatg ccctttgccc
     9001 caatcttgct ggatagtttc ttttcgttga ttgcacgaag cagaccagtt acatagtcca
     9061 ctaccacaaa agttacaagc gtataaatca aaccatcaat tcctcccaca taccatccaa
     9121 tcactcctcc accacctgcg atggcgagtt gaatccaatt ccacacttct ttcatttaca
     9181 aatcctccaa ttgttctaaa attaaatcga gcatttcagc atctttccct gaaatggtga
     9241 cttcactatt ttcaaatcca gcaaccaata aaggcagctt gtcttgaagt tcatccacgt
     9301 tgatgtgggt ttcttcgttg agcaagtctg ccttttcttt gtgatattca gttgcggttt
     9361 ctgggatgag tgaaaaggca ccgtcatgtt ggaccaaatt gccgtgctca tctttctttc
     9421 cgtatttttg aagcaagtct acctcgtctt gatagagttc atcaaccttc acctggatga
     9481 gttttgcaag cttcgttcgc actcggctaa tctttggtgg taagtcaaaa ccttttaaga
     9541 aactcatcac gtccaccaaa tcttcatttc taattttgag cattatctat cttctccttt
     9601 aaatcaccaa tctggacaca aagatcatcc acgaccatca caagcaaatc aaccatatcc
     9661 gtcccaactt tcattggctt tggaatatct gcctcgatag gttcagttac tatcacttct
     9721 tctttttctt cgttcatggc ggcctcctaa ctgtatgtgg tcattgaatt aaacgactgc
     9781 ggattgtacc aaccactcgc tgtaccgttg ctactgaaac cggttggtaa agcgattgtt
     9841 cgtcccgcaa gtttggcaca gatttcacgg atgactccaa agtcaaccca cttcccatca
     9901 tctccgagca tcagattaga accgttgaat gcaataccag cgctgccgga acttgagcca
     9961 atcttagtat aaagggaact actaaatgta atcccaccaa cagatatttg atgttgtgtc
    10021 cctatacgaa gcattgatgg taaataaatg tcgtcgtcaa agtggaaccc cttctccata
    10081 ccagactgca atgttctcgc ataccaagaa agcttctggg tgtagacact cgcagaggag
    10141 gaatctttat gagcccatga catatactct gttccatact caagatcaaa taccaatcca
    10201 cgccaatcag agtgaccact gatgttattt gcaccaattg ttccgactgt cgttccacca
    10261 cgtcgaatgg tcattccctg cgagtttatt tcagtcacaa gagtgcttcc tgaataatat
    10321 ccaatcgtag ttcctgtgat tcgaatggac gaactcgcag tcaatgctgt taggatatta
    10381 gcagcaatct tatcagccgt aatcgagttc gcgccaatac gggctgccga caaagttcct
    10441 gtggtaatct tcgccgcatc aatgcttgca atttttgcac tttgaattgt ggcatctgca
    10501 atctttgcgt ttgtgatggc cgcattttgg atcatggcag aactaatcac tgcactatca
    10561 atggttgttt gaccagtgat gtgcaccttg tttccagcaa tcaaaatact ttcaggtgag
    10621 atattgattt gattaatcac gtctccttta ccaactcgta aattgattaa gtctttctgc
    10681 atgagaattg ctgaatataa tgtgttggca ttggtatagt cttgccttgt ggtcgtagga
    10741 taaatatttc cttcatattc atcgggtttg atgctcgtgt tcactcggac tcgcattttc
    10801 aatgtgccac tcgggactgt aattgctccg ctcgaaccag tataagagat ttgatgataa
    10861 cttgaaccat ccgacgggtt tagagtaaaa ggtgtgttgc ttgaattata aatcccagca
    10921 tatgtgccag tcttaactga aaagaaagct agatacacgc tgtcagtagt aatccctgat
    10981 atctgctcag ccgttaacgt ccaagaataa gtggcattgc tgctacttgt aacggtcgaa
    11041 atctgttccc aagcaccacc tgtataaagc aagacaatat tatctgatgt atccttgcga
    11101 ccattccacg aaattacact cggagcgact ttgccttcca atggcaattt gtaaatgacc
    11161 agataatctc ctgaatttga ataggggttt gttgtcacgc cacttgtaat actcatcgat
    11221 tcaacgttct tacttactag ataactcgca atggtcgcat tagtataagt tcccgccgtt
    11281 agggtttcac taaattgttt tgccgttggg acaaaatcaa tgtaaggtaa atccatgccg
    11341 tagtaatgat agtagaaagc agcttgagtt ccgtcacgca gttgcccgat atacttatct
    11401 cccactttga catcaaggaa ctcacttcgg agataattgg ttgcggacac ttctgaaccg
    11461 tcagtggtac tcaaacttcc ttgttcccat tgattgactt taaatggaac gccgattgct
    11521 tctttggttt ctgaaacact taacagtaag gcatcagtgg tcgctgtaag ttttacgacg
    11581 ttacttatct cggataaatc aacatagcca tttgtcacat ccgttttcaa catatagcaa
    11641 ctgatggttt ctggtgcatc actagttgtt aaacgaatgc gaataaaagc ggcactgctt
    11701 ggaacagtaa gcggtgtggt ggtgtttcca aattgggtgt acgataaaaa ggacttatcc
    11761 tctttgtaat aaagcagata agccgttgcg taagtagagt aaagtttccc acccacaaat
    11821 gtttgaagta gatacttttc gccatttcta actcttgtaa aacctgagcg aacgtatcca
    11881 ccataggtat tttcttttcc atcatttgta ttcagaccac caactgccca ctgcaaatct
    11941 gcaatgctca taccggctaa aacattgacc gctttatttg ttttagaaag agatagctca
    12001 aatctatcag cattttgggt gatggtgctt tctgcagttg taatacgccc tgtcagatta
    12061 tcaaccgtcg attgacttgc cttactagca atctgcccag cttgcactgt cagagttgat
    12121 tcagctgtga ttactcgtcc ttcaagagtt gacataccac tcgccactaa tgaaatcttg
    12181 ctagtattct gttcaacgga tgtagtaagc gtttcaacac ttgcacctac actttcagct
    12241 gcattcaaag cattcacagc atcgttaaat gcttgattgg ccttttcagt ggcagtattg
    12301 gcatcctttt gtgcttgttc cacggcttgc tttacttctt ctgccgtcat atctgtcata
    12361 agtggatacc actgagtgac gccatcacgg atttcataca cccacatttc aaacttgtca
    12421 ccattttctt tgtaccaaac gtcaccgagt ttcggattat ttggagttgt cgggccatag
    12481 aagttggtgt ttttaccatt tgccgaactc aatgcgtaat tggcatcgtc tttggcttgc
    12541 tccgccttgt tgtccacccg gttgatgtcc ttcgccacat ccgtgaattt tggcgtgatg
    12601 tttccgagtg ttactgagat atacgctttc agaaatggat cgtatttgta tgagaccatt
    12661 ctagctgtga tgttcaatcc atcttcggca tgaataactg aaacagtatc acccaaatta
    12721 atcgtttcaa gctgtgcaaa atctttatat tcttcagttc gctcaagcgg ggcaaactcc
    12781 acatcatagg tggcttttgg caaatcgacc ttgttagctg agtattctaa cccagccaat
    12841 ctgcgaagtt catcataggc ctcggcttga gtaaactcat cctcttcatc accaactttg
    12901 acgttgtcgt acttgatgac tttgatttta ggctggacgt attggttgat gtttggactg
    12961 tccacgtatt tttcgggaag aaatagtcca tcataccctt gtggcataat gcgagtaaca
    13021 attccagagt aatcaacatc agctttatat cctgtcagat tcttcttgtc tcggatttgt
    13081 acgccgttgt tactgccacg agtcgttaac attgaaatcg tgaagttatc gcgtacaatt
    13141 tcaccaccat aactgctttg aaaaccattc tctaggtcac tatcaagcaa cacctctaca
    13201 ggatttagtc gcaccaagcg gacattattg accgcactga tgtttgatgt accggtaaaa
    13261 cggtgcatga attgagttgc accaagcagt tgtgtcaaag ctcccgaccc gttcttgttg
    13321 acgatataag tatcttcaat aaagtttgtg gctaggtcat agaaaatatg atgagctact
    13381 atgtgcaaaa aaccaccaag tgcatcatct cgttcaacaa tcctgaaaag ctggtcggac
    13441 agttctggta ctggtgcttt cacaatcatt tctggcaaca attcctcact gtgtggagca
    13501 tgaattggat aatcgaattc aaaggaaaag agtccgttca attcctcact caccacgggg
    13561 ttcagaatgt tgccatcaag gacagcgaga ccgttatgat tgaaatttga ttctgttttg
    13621 ttgtaaagcg taatcataag tatcgccacc tcggttctat ttctactttg gttatacctg
    13681 taccaagagt cacagtatta ctgccgactc gaaactcagg aaagtcacct gtcatgtatt
    13741 gattggctgc cacatccccg taaaagcatt ctttcaaatc cgaatctagt gtcaggttgc
    13801 cattcgtcag atttaacacg ataggcttgc catttatcgt gagtgtccta gttccattgc
    13861 cgtagacctt aatcttcggc aatgagtaaa tgttgccggg attattgacc gttcctgacg
    13921 tggttcgatt gatagtcgct acattggtct tataacgaaa aggcgaacag gttaactcca
    13981 tttcaaactc ccacatggaa gataaaatct gagttagtcc gctcgcctta gcatacttga
    14041 ttgggtaaaa cacgtttggt tcatttgaaa atgcgatagt tttagcgttt aggatttgtg
    14101 gcaaaattat ctgccagcta gtccaaacat cagatttcca aatcactacc ttaaaaccga
    14161 aagtcacatc tttccagcct ttcagaatgg tcaaatctcc ttcacgtcca tcaatctcaa
    14221 tactatcaat gacacgttcc gtcactggaa taactggcac ttccgtaatt cgcaaaccta
    14281 aatcagtccg tgaattaatt gttccgtcta aaataaatgc gttcaattga ccaccgccca
    14341 tctaaataac taaaaaagga ccagacaaca atgtctaatc ctaaagttta taatctatca
    14401 tcctattcgg ttgtttcttc cagccctatt ttcttggtaa tcgctaaata cgcgtataca
    14461 accagcacgc cggaaattcc atatattgca tcgattagat ctggtgtatt cagaaaagtc
    14521 atcatcgttt cacatacaat atttgcaaca atcagaccag ctcccatcgc agtaagaacc
    14581 cttaaattcg ttcccataag caaccaaaga tagcctatca ccaaatagaa aagaaagctt
    14641 aatgcgaagt ttgaaatgtg agaattaatc tgattgttga tgaatacaaa accatgattt
    14701 atgccgctat accatcttac tgccgttaaa aataatccaa tagcaagcag tagaatatat
    14761 cctatcagtg caggtttctt tgctttaaat ttatgcttca acatatgatt accaccatct
    14821 cttcagtaaa cactcgttag ttatagtata cagtcaaaat gtgaaaatcc aacactaata
    14881 tgctacacgt ctacgattca acgtcgccaa gctcttatca atttctggag caagtttgcc
    14941 gaccaatgtt ccatcattca agacgacttt aatgtccatt gctttaagta gcgttggcaa
    15001 catttcactc gtgatgtttg ttaaggcatc aaccttagca cctaaatcaa gcatcgagaa
    15061 tgtgctatca acggcagcgt ggatatcggt atcaatatca aagcttggca tataaagttt
    15121 tgttggaata gctttctgca tcgttttaga aacaccagtc atttccttat caaaacctac
    15181 gccaatccct tgtgccatgt acttcccgat ttcatcacga aacagtgttg atggtgaatg
    15241 aataccaaag aagtccttga tgttgtctac tacaccgcca aagaatcctg aaatcttgtt
    15301 gcgtaaccaa cttccagcat cagcgatacc attccacaat cctgtaatca agttcttccc
    15361 gatagttccc atagttgaaa catactcacc gaaagcattc gcaagccctg tgatgatttg
    15421 cggtactgct ttgacaatgg cagaaatgat tgctggtaag ttttgaacta atgccacaag
    15481 caacttcaca ccagcattga tgattttgtc gatgttgttt accaaagttc ctgttattcc
    15541 gttgataatt tgcggcatgg cattcgcaat cgttgtgata atttgcggta acgcaccaat
    15601 caatgccaca agtaactgaa tacctgcgtc aatcaactga gggattgccc caatcacggc
    15661 attgatgata ttttgaataa tcactggaat agctgcaaca atcgcattga tgatatccgg
    15721 caaagctgtc acaagtgatg tcaaaagttg aataccagtc tgaatgattt gtggaatagc
    15781 actgataata aagttcacga ttcctgtaat aatctgtggc agactttcaa tcagtattgg
    15841 aagtgctgtg ataagtcctt gggcaagtcc catgataagt tgtaaagcag catccaacag
    15901 taatggcaag ttttcaatca agccgttcac aatcgttatg attgcctgta ctgtaattgg
    15961 aataagttct ggcagcgctt cgcctagtcc ttggataagt gttgtcacaa gttgaaaggc
    16021 agcgtcaatc agtaaaggta agttctcaat aagtgtttga acaatcgtta aaacggcttg
    16081 aaccaccact ggaataagtg tgggaagtaa gccaagtatc gtttcaagca cttgtgagaa
    16141 taatgaggtt actgcttcta agagtgttgg caataactgc cccaatgctt gaataatggc
    16201 atccaccact gccggtaaag ccgacacgat attttgaatg accggtagaa tattcttgat
    16261 gacgttttga aattgttcca ccacattgcc aatcaacata ccaatgtcag cgctgctgtt
    16321 accaagtcca gccatcaagt ttgagattgc agacttcata ccgtcaatcg aaccacttat
    16381 cgtctcactc gcttccttgg cggttgtacc agtgatgccc atttcggttt gaataacgtg
    16441 aatcgcttca gtcacatcag cgaagttcga gatatcatat ttgacaccag aaatcttctc
    16501 agcctcttgg agcaaacgtt ccatttcgga cttcgtgccc ccaaaaccta atttcaagtt
    16561 gtccagcatc ccgtaatttt gcttggcgaa accttgatag gcgttctgga tggactcaat
    16621 atccgtcccc atcttgtttg cattatcgct catatcagta acagcttgat ccgcaactcc
    16681 tgcggctttc ttggtatcac caccgagaga tgaaatcaag gcagctgaga aacctgtgac
    16741 agtttccatg tattcattgg ctgacattcc ggcagttttg aaagcattgt cggcattctt
    16801 ttgaacggtg gatgacgcat ccttgaaaag agtatccacc ccaccgacta attgttcata
    16861 gtccgcgtaa gcagatacca cttgttttcc tagctccacg gcagcagcgc cagcagcaag
    16921 tgccactgta cccattgcta ccccgatacc ttttagaacg ccaccgaact tttcaaattt
    16981 acttccagac ttttcagcct cgtcacctgt atccttgagt tcgttgccaa gctcatcagt
    17041 tttcttagca gcatcaactt cttcagtacc catggcgtca attgccttct cgttgttagc
    17101 aacttcacgc tccatattat tgagttgggc ttttgcattg tttaattgca ctgtccaatt
    17161 ctgagtacgc ttatcgtttt caccaaaact gtcactagcg ttcttcaaag cattttcaag
    17221 ggtggtgatt ttttctttct gggcatcaat cgatttgttt aagacttggt tgcgagcaga
    17281 cagtgagctg atgcttttat cgtttttatc aaattcggat gataccagtt tcatttctga
    17341 acctagcacc ttgaatgatt ggttgatttc acgcaacgag ttcttaaact ccttttcacc
    17401 ttcaacgcca atctttaaac caaaattatc tgccaaataa atcaccacct tcccttaaat
    17461 tccactcggt atcacatcat caatgaataa ctcaactttc ggcttgctaa tcccagtaaa
    17521 ttgcttatga cactcccata aatccaaaaa cagtccaagc gagcagtacc agaaatcatc
    17581 tgacgtcata cccatttgaa ctgtcccata gtaatacagg cgagtaaatg tttcttcatc
    17641 acttactcgc ctgctttgtt ttttgaatct gtttcacttt cgatattgcg agctgtgccc
    17701 ttgaacattg cttcagtgat tgctgattta tattcagcca actgcaacgg actcgtcatc
    17761 aactccacat attcagtcgt gagttcatct ttcttttctg tgttcttcaa gttgtaaatc
    17821 ttgatggact gattcgccaa cagcgtaatc aaccagatga tttcatcaag cgccaattca
    17881 aagttctcgg atttcaacaa tttctcacca aggttatcaa gcccaccgta gtgactggcg
    17941 atttctttcg tggcttttgt tgtcaaaatc agttcatatt catctccgcc aaggctaatc
    18001 ttggcactgc gttcttcact cattgttgcc tcctagtctg ctgtgaaatc tggttcataa
    18061 acactcgtgt accatccatt gatagttgtt tgttcaactc ccgtatcgtt ttcatcaact
    18121 tctgctttcc atggatgctt accactagca tctaacttgt tacgacgcat gactgtaccc
    18181 tcaattgttg gtgttgagaa tgtaatgtcg tcaccttttg ttgctaggct tgtgctgggc
    18241 acggcaaact tcactcggta caaccacaaa tatttgtatt tgccattcga tttacgagca
    18301 cggaaaccaa tcgcaactgg tgtgccacca tcttcggttg tcgaaattaa gacaccgttt
    18361 tgatccacgg ttgcaccaac aagcactgcc gccacagcac gtccaatatc atcaacaccg
    18421 agcgttaact ttccattttt aaattctttc actacttctg atgcaccatc atcggcgtat
    18481 aaagttgctt cagcaagttc caccgaaagc tcagctgaga ttgctttcgc aagcatagcg
    18541 ggtgtgccgt atgtttcttc gccagtagtt gccgactctg taatcggtgc ataatataat
    18601 ttatccaatc caatcgttgc cattttcaga cctccatttc ataatgcttg gcgacatcaa
    18661 tcgccacatg atagtaattt gtatcttttt caaatcctac aaataatcgt tcagtaatcg
    18721 taaactcgtg tttcaacaag ttctgaacca gttgtttttg cgtgttcaga tagttgcctt
    18781 tcacaaacaa ggaaatgcga acttcctgta catcaacggt tggaaagtta tccccgtaaa
    18841 catcgaacct gtcagcgagt ggtgtcagca cacaatactt gtctggcgct ttatcactaa
    18901 actcgcccgt ttcaacagga atatgaagtg tggcaaaaag actgttcaat tcctctaaaa
    18961 tgttcaaatg ccgtttacct cactttccat ttttgatttc attgtttcaa tgcatgactt
    19021 gcgactttgt gattttgcag gtttcaagaa tggctttgct ggctgaccgt gttttccata
    19081 ttcgagaatg ttcgcaatct tagcatttga actgccatcg attcgaggct catcaaaacc
    19141 aaccttgatg ttccagttgc cgcttttatc ttgacgagct tgtgtgatac caagggctga
    19201 ttcaagttca ccagttgaac gacttggtac ttttgtattt gagccaacaa cggcagttaa
    19261 gttacttcgg actttgcctt ccaccacttc agcaccactt tctaaaacct tggggatgat
    19321 ttcatctgtc ttgttttcta gttttgaaac ctttaacaag aaatcttctg gcatcttcat
    19381 caatgcttta gccatccgca ctcacctcca tttcagtagt cagcatttca aggtacatat
    19441 tgcgatttcg gacattctcc accgacacaa tctgatacct tttctgatct gaaatgacaa
    19501 ccatctctgt cgtaacctct agattaggaa tataacgaat ccgaaacagg caggtcgctg
    19561 aactaaaagt cgccatgttt acccatttct cagtcgcatt cttttcctct ttgtatgcat
    19621 ggacagctgc aagaacttca tcttgtttca ctgaaaatcc agcgctatcc ttttggttcg
    19681 tcactttaac gatttgtatt cgttgattca tctttccaaa actcatactt gccaccgcct
    19741 atcgagcctg agtaacagat tgaccgtttc ccagacttgc ttactggcat tgacgttatc
    19801 cgcaaagaag ccaccagttg aaccatcacg gctttcgtaa aagttactgg ctaacacaat
    19861 aaccgcctgt tcagtggttg gcggcatttc atgttctgaa tagtagccag actcaatatg
    19921 ttgatagctc tcagcataag aaagagcggt cagaatcaat cgttccaaca gctcatcatc
    19981 ttcgttatgc tcaagtatga ggtttgcctt gaccttattt agtaagtcac tcataggcta
    20041 ctccttaaga tgaggcagat gctttcatct tcaagatttg cactgcttca ggtaacacaa
    20101 gtttaccgtc cacacgttct ttagctaaga aaccaaccat gccattacct gcaaagagtt
    20161 cacgcaactg gtcgaatgaa cgcacgccac ggtcgccaat gttatagtag ctgaaatcac
    20221 caaaagcaat gactggcgca cctgccttaa cggttggaac aaactccgaa gtataaactg
    20281 gataaccgag cagacggtca ggttcacctg ctaccaaagc tggctgccac atgtaggcac
    20341 cattgttgtc tttcaactta cgaagcaagc tgatggtttg gtcgttcatg ataaacttcg
    20401 cgtttttacg atatggacgt ttcaaagcat acacaaggtt caccacatca tctgccgtaa
    20461 ctgcactttg agtattagtt gtaatggcaa cttgtccgcc acctgttgct gcaaaaatac
    20521 cgagtggttt accaacaccg tcaccattca agaacgcatc ttcttccgcg tttgctaagg
    20581 ctttggtgaa ttggtcgatg atatagtttt caaggttaaa ggcgttgtca taaagcaatt
    20641 catcagtcac cttgattgcc acatgaagtt tgtgagcatc aagcaagatt tgatcgaacg
    20701 ttgcatcccc aaagctcaac tcgccacctt cttcaatcca tgaagcggct ggtttagaac
    20761 ctgcaatatt aatcttgtgt tcaccacttg tcgtaattgt tgtcgcaagt gtacgcatga
    20821 tgttttcaga agtcatgccg tcaatcaaac gtgagtcata ttcttctggc accaaataac
    20881 cgcctgccgt atccacacct tcttgaagaa catttgaaac atgtcggaag tttgagcgca
    20941 aggcttcaag tacaccttct ttatattcag ctgaagcacg tcctgttttc ttaggttcca
    21001 ttttattttc agtagtcata ggttgcgaag ttaatggttg gctcattggc ttttcaagtt
    21061 cacgatccat cgcttccatt gactgcatac gttcaatttc catcgtaaag ttcttcactt
    21121 ttgtttccat ttccgcataa gtcttggcat cttcttcaga catcaagcca tctttatctt
    21181 gctttgcttc tacaaaagcc tttgctccgt tccaagcttg tgcacgtttt tccatcaatt
    21241 cttgaatttt gttcatgggt tagttcctcc aatttttcat taaattaagc cgactcatta
    21301 acgaatcggc tgggatagtg ttattaattt cttctcgttc ctttggttca ggtggttcaa
    21361 tacgacactt cttagcaatc gctgaagtca gtgaattagt tactttgact tccgagtaca
    21421 tcgaattatt aaccggaata tccgggtcaa tctcaccgat gatttcatcg gcaaatccta
    21481 ggtgaacagc tttatttgcg tccatccacg tctccgcatc catcatatgt gccagttgca
    21541 cacgcgataa attcgtttta cgttcatagg cgttgataat acttgctttc acttcatcaa
    21601 gcatggcaat ggctttctcc atatcaactt tttcgccaaa cgcaaccgct gacggattgt
    21661 gaatcatcac catcgccaca ggactcatga gtaccttgtc acctgccatt gcaataacac
    21721 ttgcggccga agcggcgatg ccgtcaatct tgactgtgac tttacctttg tagtcagcaa
    21781 gcatgttgta aatacttgcg gcagcgacac aatcgccacc acccgaatta atccagactg
    21841 taacgttacc tccacctgaa tttaactcat ctttgaaaat ctgcggtgtg acttcatcat
    21901 caaaccagct ctcctcagcg attggtccgt tgaggtagag ggttctctct aggatttcct
    21961 cttggttgtc ttgattcaga accgtttgat tcttccactt ccaaaacttc ttcatcttgt
    22021 tcctcgcttt cttctttgtt tacgccataa gctgccccgg cttgttcaat tggcagcata
    22081 ttgccattga agtaatattt cgcaccagca ccaccaggga ttgggttcaa gtcctcaagt
    22141 tcacgcacat catcaggaga taggaaccca ttttgaatac cggttgcata gccttgttga
    22201 cgtgtggcat agtcaccacg caataagcca tccacattaa actttatgaa atacttttct
    22261 ttttcatcgc gacttaacag tgatttatta agtgcttgtt cccaacgatt gagccatggt
    22321 gataatgaga acttcacaaa atccaaactc aaatgttcaa cgtttgagaa ggtcgcgtgg
    22381 tctaagtcgc caaccatgtg aaggggcact cggaatatcc gtgcgatttc ttccagttga
    22441 aacttacgtg tttcaaggaa ctgggcttgt tctggtgaca tggaaatagg cgtatacttc
    22501 atgccttctt caagtactgc aaccttggcc gcatttgaac tgccaccaaa tgtcgcattc
    22561 caactttcac gaagctttcc tgggtctttc aaagttccag gatgttctag gacaccacca
    22621 ggattggcac cattggcaaa aaatttagaa ccatattcct ctgttgcaat cgcaagcccg
    22681 atagcattct tagccatggc aattggtgaa taaccaacca agccatcaaa gccaaggcca
    22741 ggaatgtgaa gcacgtcagc ttttgaaagt ctgacttgcc cgacatcagc ttgatataaa
    22801 taatagattt ccttgttgtc atcgcgatta accgtcatct tatctggcat taaaggatac
    22861 aaagctacaa cttcaccttt gccatttcgg ataatttgcg cataggcgtt accccaaagt
    22921 agcaggtgag tcattaaggt ttcacgaaat acaaatgatg tcatttccga atttggttca
    22981 tcatgcagca agaaatacaa cgaatgtttc attgctttat ctttgccgcc agcatctcgg
    23041 tattggtaaa agtgtaatgg caatcctgcc acggcttctg atagcacgcg cacacatgag
    23101 tacacagccg tcatctgcat agctgtttgt tcggtcacgc ttttaccagc tgatgagccg
    23161 ccaaacataa atcggtatgg attcatgatg gtttgatttg ttggtttatc gcgtgatttg
    23221 aatagtgatt ttattatgcc cattgattgc tcctttcaaa atttgagtat caaaaaagca
    23281 cctacgattt gtagatgctg tgttcacatt agatataaaa ccgacttcct cttgacaaaa
    23341 ttatctcaat tgcattattt aaaaaggtat ttacttcctc taaacatttc gatttttcgt
    23401 caggtgttaa ggattttctt tgataaggaa agaatgaact acttggtgca agttttttaa
    23461 acgtcatata atcttcatca tcgaagaaat cttgtaaata atttagttct ataaaacttt
    23521 tccaatcttt gaatttagag aaatagtctt tattattaac gataaattta tcagcgttag
    23581 ttgatgaacc atatcgttta tctcttacat cagaataaaa taaatcagga aagtcatgat
    23641 acggcccttt acctttaaca ccattgagag aaggttttga tacatatagt ggcataaaat
    23701 ttgcaagagt atgacttaaa tcatagaatt ttacaaattt cactttcatc tcttcggtga
    23761 tatcatcgga atataaaatg actttcaaac gcccataatg actatctggc attgaaaagt
    23821 tcttttgatt atagcatttc aataaccgta tcgttgcacc agcaattgtg ttgaaagaaa
    23881 taatcgtttc tcccctataa ttcccaattc cttcagcata ctttgtcaaa tcttcagtta
    23941 gaatttttcc ccaccatttt ctataaaaca gtctagaaat taatgtgttg tccgggtctg
    24001 aagatatttt atctggtgct acttgaatca aatattcatt catacacata cacctccaaa
    24061 gacaattata tcaatggatt gtaataaaag ctcatatttc acagaaacaa caatccccta
    24121 tcatcataaa cactggcacc attatcattt ccacaacgaa ttgcacgatc cagagccata
    24181 attgtcgcga tggcaccatc aatcttctca gtggattttt ctttatcagc tttgatatta
    24241 cctgccgggt cagttcggac gaaaatgttg tccatattcc aacgaagaac cggatgaccg
    24301 ccatgggcta atttctgttc aagagtcaac ttcatcaact ctttagtagc cgggctcata
    24361 tccttgaaac cttgaccgaa aggaactaca gtaaatccca taccttctaa gttttgaacc
    24421 atttgaactg ctccccaacg gtcaaaggca atctcccgga tattgtattt ctcacccagt
    24481 tcctcaataa atttttcaat gaaaccatag tggactacgt ttccctcagt ggtttgtatg
    24541 tacccctgct tggcccacaa atcataattc acatggtcgc gtttaactcg taagtccaag
    24601 gtatcttctg gcacccagta atatggcagt atcacaaact tgtcatcctc atcttcaggt
    24661 ggaaacacca aaacgaagga tgtaaggtcg gtcgtgctgg aaaggtcgag acctccgtaa
    24721 caaactcgac ctctcaaact ttcttcatct gcattgaatg aacaagcgtc ccatttatcc
    24781 attggcatcc aacgcactgc ttgtttcacc cattggttca ggcgaagctg tctgaatgag
    24841 ttttcttcag ctggattttg tttagccgaa tcacaagcag ccaccacctt ttcttctgca
    24901 atcgttatcc caagtgacgg attggctttc ttccaaactt ctggatcagt ccaatcatca
    24961 tcttgcttga taccgtaaat taacggatag aatgttttat catgctttcg accttcaata
    25021 atgtcgagag ctttttgatg ctgctcataa caaattgaat tggtatcatt ccctgccgtt
    25081 gtaatcaaaa agaatagcgg ttgcgttcta gcatcccctg cccctttcgt catgacatca
    25141 aacagctttc tgttaggttg tgcgtgaagc tcatcaaata caaccgcaga actgtttaat
    25201 ccgtgtttag agtatgcttc agctgataat acttgataga aactattcgt tggctgataa
    25261 ataattcgtt tttgtgaagt caggattttc actcgtttgc ttaacgctgg gttctggcga
    25321 atcatatcaa cagctacgtc aaatacgatt gaagcctgcg ctcgatcagc agcacagcca
    25381 tatacctcag cgcgttcttc cccatcaccg caagtcaaaa gcaaagccac ggcagccgct
    25441 aactcactct taccattctt cttaggtatt tcaacataag ccgtattgaa ttgtctgtag
    25501 ccatttgcct taatcgttcc gaataagtca cggataatct tttcttgcca aggcatcagt
    25561 ttgaacggtt tcccgaacca ctgacctttc gtgtgtttca acaactcaat aaaggccacg
    25621 gcataatctg cacgtgactt atcataatgg gagtctttgt ccataaattc agttggtttg
    25681 taattatcga ttggaactca ctcccttcta cgcaaaaagc agctgttgtc agctgcctta
    25741 atctctttat tccatcatat cggcaatgaa ctgaatggct tcagggttgt tcttgttatc
    25801 ccgcagcaat tgcaccagtt cttgccaatc gtagtatcct tcttcaatgc catcttcatt
    25861 atctggcatg taaatttgat aatccatcat ttatcaccct ccaagataaa gcggctgtac
    25921 tcatctttgt gttcttcaat gaaaatcacc aactcgtaaa agtgccggtc atgtgcaatt
    25981 cgctgcactg caatcgcatc aaacatattt gtcagaccac tttctgcaat ctcaatgatt
    26041 tgttgcttaa tctttgcgtt cattttgacc gtcctttctg cctgcctcat aggccgcaat
    26101 taaggcatct tccaaacacc aaaccgcaat gtccaagaag tcttcacggt cgctgttgtg
    26161 ggccttcagg tcgccacgtt caggcaactg aacgatgtaa ttctttgcga tgttgtaaat
    26221 cttacgttta ctcactttca tcttaaagcc cctttctgat gtagatggta tcttctgttt
    26281 ttgactcaag gtcaacccag accaaatcgt ggttcaatat catgtccgtc acgtattgcc
    26341 aagcatcatt gaaggtttca aatccttctc ttctaccgtt ttgaaagacc aattcgaagt
    26401 ctggtttgat gatgtgcacc acatcttcgc cgtataccgc gttcagtgtt gaaccgttgt
    26461 cccattggat aaggattgaa ccaatgtcgt caacaccggt tactgtgcct tgtgtgccct
    26521 tcgctggggc atgtgggtcg ttcatcttga tgagttctac acgactacct tcagggtaac
    26581 gttttttcat accctcgatt tgttcatgag ttggaaacat aactttgtct tccgtattct
    26641 tttggtagtc tatatatcac tctaaaggga ttttatagca agtgatacgt tatttattca
    26701 ggattttcta ggtcattaaa tgcgatagtc tttccatctc gaatcactga aacgtctgca
    26761 tcgcttccag cctgctcgat ataacgctta acaatcacat cacagaactt ttcatccagt
    26821 tcgatcgtat ggcaaatccg gtcggtctgt tcgcaagcaa tcaaagttga tccgctgcca
    26881 ccaaaactat ccaacacgat gcagttgctg agtgacgaat tggtaatcgg atatgccaac
    26941 agcggaattg gtttcatcgt tgggtggtca ccattcttct ttggtttatc aaattcccag
    27001 atggtcgatt ccttacgtcc ggtgtaccat tgatgtttgc ctttcttctt ccagccatag
    27061 agcaccggct catgctgcca ttggtaaggc gaacgaccaa gcactagact ctgtttcttc
    27121 cagatgcacg tacctgataa atagaaccct gcatccacga atgcttttct gaagttcaag
    27181 ccttccgtat ccgcatggaa aacataaata ctggcgtcgt tggccatcac actttccata
    27241 caagaaaagg catcatacaa gaagttgtaa aatgcctcgt ccttcagatt atcattttta
    27301 atcgttcccg ctttaccttg atatgagacc ccataaggtg gatctgtcac cacaagattt
    27361 gctagtttgc cgtccatgag tttctcataa gtttcaggtt ttgttgagtc accacaaact
    27421 aaacggtgac gaccaagcaa ccacaagtcc cctgttttac tgaaggctgg cttttcaagt
    27481 tcctcatcta catcaaagtc atcatcttgg gcatcatcat taccgttgag taatttatca
    27541 agttcatcag tttcaaaacc aagcaaatcc aaatcaaact cttcaccttg caggtctgat
    27601 aactcaaccg ccaacatttc atcatcccaa ccggcgttca ttgctagtcg gttatcagca
    27661 agtatgtacg cacgcttttg tgcctcggtt aggtgttcca caaacacgca cggtacctgc
    27721 ttgatacctt cttctttagc cgcaagaata cgtccgtgac ctgcaatcaa gttataatcc
    27781 ttatcaacaa tcaccggatt tatgaaccca aactcacgca tagaagcttg aatctgtttc
    27841 acttgatcct tactgtgggt acgggcattt ctggcataag ggaccagctt atcaatatca
    27901 actttctcaa aacgttctgt tgtgttcatt agaacaaccc ccattctgct agtttctcaa
    27961 acccgccaag ctcattcaca taatcacgcg caacttccac gatttcagaa taaggcacgc
    28021 catcaatctc agtgtcacca atcgcacagc ttaactcaac gggcatacca gtttcttgag
    28081 ctttcaagaa tgcgtagatg ttcactgaca catcagcttt tgataagtct ttcccatgga
    28141 taccgccacc tgttactccg tctgccatat cggaaccaag cttgcgatta gtcgcacctg
    28201 tatccacatc cgggccgcca gtccaatcac ctagagggtt gactgtagcg tttggatatt
    28261 tttgttttaa ctcatttgtc tttgcgtggc tctgacaaat tatcaagcgt tcaccatcaa
    28321 gaatgtactt tccatcaaat ggatggtcct gatataagtc acgcgcaatt tttgagagct
    28381 gtaactgttc atcagtcaat ggcatcccac gaaagattcc attatcgcca caacgaattt
    28441 cagtcgcttg attgtcagcc aactctttat cttggggcac aatcaccaca tcttttttaa
    28501 ttttgcctgc gatacgatta atcgcttggc tgatttcatt caaatttaaa gcagttgaag
    28561 tttcaataat cacatgacag atgccatgac caattaaaac ctcaactgct atctttggat
    28621 tattttcttc tgcgtatgcc aagtccacaa tggctccggc aatacgatct gcaactttat
    28681 ctggatgttg cggattaacc ttctctatca taaattctgt tcctttcaac cattcttaga
    28741 acgtaacaat cgttccatcg catccgtatt cggatctcct gtgtattcca ctgatgcatt
    28801 ctcttttaca atctgttgaa tctcatacca cgtcacattt gcttgctttt ggaagttctg
    28861 cgacatcgcc acaaatgggg aggccatgac tgccttggta gtcgggtgtt ttccgagcaa
    28921 accatattga ctcagagcat tttcacattg aacaaaacgc gcaaagttct gagcgtagga
    28981 ctcaaccatt cgtggggcta tcaacgtttc gcatccgcgg tctttcaacc attggaacgt
    29041 tgagttataa atttcatcag cgcctagcac agtgccatcc ttctgttttg cagaaaggta
    29101 agcacttggt tcaggataat cattgccctc aagctctaca ccctcaccaa tatcttcacc
    29161 aattaataat tcaacatccg gtaagtcaaa ttccatccgc gatgctttct ttccagcagc
    29221 aactttatca acgagtggct gtgagcgttc ccctgaacga ggtcgtctgc caccgcgttg
    29281 tgttccatct tttgccaaaa tttatcacct ctttttcttg tttggggcta tcccccgttt
    29341 gaactgaact tttttcgcgt aagggcctag cgcgctgtac agtttagaag ggtttaggga
    29401 taattaccgc ccctcccccc cttgttattt ttcacatgat tgttttatct tttgtgtttc
    29461 gtcgagtcat gtgaattttc gagtgacatg atttgcagac actcatcaaa ttattttcat
    29521 catgcgtacc accatcactt aattccttga tgtgatgcac aatctcaacc gaagtaaagc
    29581 gtccttcctt cagacacatc tcacataggg gatgtgctgc cacgtacctt gcccgtattt
    29641 tcttccacgc tctgccgtaa cgtttgttga ttgccggatc acgctcgtat ttctcgtaac
    29701 gtttcctcgc aagtgtttta tgttcatcac aataagtatc atgcgtacgg ttcggacaac
    29761 ctgggaactt acaggggtta gctggtttca ttggcaagtg gggtcacatc ctttcgggca
    29821 aaagaaaagc ccttgaagga tttttcccac aaaggcttcg agtatttgtt cgatacttct
    29881 attgctggta ttgcttgagt atcgtttgtc ttttttgaca tcttaatgat atcactaaag
    29941 gctactgaca ttcactgaca catttcaaat gactatgggt tcatctggta gcttcaagtt
    30001 ctcaagagct ttgctatgcc aacgaaagac tgtccgcacg tcagcgtgaa gatcttcagc
    30061 aatcttgtcc cagctctctt ccaacaggta tcgattgatt aacacaatct tttcgtccac
    30121 gtttgtgagt tcatcaatca cgccacgcat ttgtcgtttg aggtctacaa acttatcaat
    30181 ctctgcatca atttcagctt ccaattggta acacttctca aggttacgaa caaatggtgc
    30241 ctcagtattc cgtgtgccac ttgaaatctt ttcttcgaat gttgtggaag aaactgtcac
    30301 cgccagttga tagagctccg ccttctcttt aatccggtcg tcaattcgtt tattcaaacg
    30361 gtacgcttgt ttcaagtatt cttttggtgt cacttgcttc cctccaatct agctttcact
    30421 gcatcaatca aacatgattg tgttttatct tttcgtttca aagccaacat cacatcttca
    30481 tcaatcgtat ctttcgcaat aatgtgatga atgaccacag tatcactttg cccttgtcgc
    30541 cacagccttg cattggtttg ttggtaaagt tccaaactcc acgtaagacc aaaccataca
    30601 agtgttgaac caccagcttg taaattcaat ccatgtcctg cactagccgg atgaatgaca
    30661 gcgatgggga tgctaccttc gttccaatca ataatgtcct gagacgtttt gatctgtcga
    30721 acgttgaatc gttccttgat acgctccaga tcatgctgga accaataggc aatcagaatc
    30781 ggtttgccat ttgtaccttc aatcaaatct tccaaagcat ctaacttctg gtcatggatg
    30841 tgatgactca cattaaactc atcatagatt gctccattcg ccatttgtag cagtttattc
    30901 gaaagcaccg ctgtattggc tgcatcaatg gtggcttctt tcaattgtag caccaagtct
    30961 gccttaattt catcgtactt ttgcttttct ttgagtccca tatcaactgg cacttcattc
    31021 atcacaagct ctggcatatc caagaaatcg actgacttca ttgaaatcgt gatatctgaa
    31081 atcttgtcgt agatttcttg ttctgcatct tcccttggtt tccaagaata aatctgcatg
    31141 ccattttgct tgtcaggtag aaagtattta tctcgataac gtgaaatata atagcccaat
    31201 ctctcgccca tatccagaac cttgaactcc gcaaacaaat ccatcaaacc attacttgaa
    31261 ggtgttccgg tcaatccaac aattcggtca accttgtgtc tgactttcat aaagctagtg
    31321 aaccgtttag cttttaagtt tttgaaggac gacagttcgt caatcaccac catatcaaaa
    31381 tcaaacgtaa aacccgattt gttaatcagc cagtccaagt tctcacgatt gatcagatag
    31441 atgttggcat tattttgtaa tgctgccatt cgctgacttg gtgttccaag aaccacggaa
    31501 tacgataaat ctttcaaatg atcccacttt tgaatttcag ttggccatgt tgattgcgtc
    31561 acgcgcaacg gtgcaataat taagacacgt tgtattgtaa agtcatcatg catcaagtct
    31621 ttaatagctg tcagcgttgt caccgtttta cccaagccca tgtccaatag aagggcggca
    31681 gttttatgat ctttgataaa atcaatcgca tatttctggt agttatgtgg tacgaacttc
    31741 atcagacatc acctcctatt ctttctagta actcaggtat ctgatcaatc tcatcaagca
    31801 cgaaacactg aaaccctaaa tctgtcagtt gtttcatacg aaaaagctgt aaagaccgtg
    31861 gcttcttccc tggcgccttc acttcgacaa atccaagttt cccatttggc ataagtacca
    31921 atcggtcagg cacaccagca agccccggtg atgtaaactt cgggcaaata ccacctaccc
    31981 ttttgacggc tttcactagt gcttgctcaa cctgcttttc tctcatagcc acaccgtcaa
    32041 atactcgaaa tacgcatcac gcaagatttc caagtattcc ttttctgcgc agtgaatcat
    32101 cagatgtgcg acttgcttat cgaatgattt cttccaagga tacgtgacat ccgacatggc
    32161 aaactcgtaa aactcatcaa atcgtaaatc acgtttaccg attccaattt gtaaccactg
    32221 tttaaatttc atttagatat tctccaatct gcaagtatgg tgtaacccgt gaatacgcaa
    32281 tacataactt tatataggtg gtaatttttt atcccttaag aaaagttcta tataagacct
    32341 acacaggtta cactttcctt taaaatcaac tattccaaga actcggattt gagttgcaaa
    32401 ccaacaatct tgtttccttt attagttcgc cggcgcttaa atccgcccgc ctccagagcc
    32461 gtgtaaaaat cggctgaact acgaatgtac tcaccagttc gcgtacaaaa cgcacgatac
    32521 tctgcataca actcaccaga tttttcttca aagtcaacag cagtctcgca gcactcgtct
    32581 aagaaatggc caagccaatc attcgccgtc ttatattcag aaattgcatt agcgacaact
    32641 tgtggaaccg ttaaatgaaa gtctgcggcg atgacttttt tcgcaccgtc aaggatccat
    32701 tgcaaaatgg ctccgccggc attttcaaaa aggtagtccg cataattctt gatgtcctta
    32761 ttgccgtcaa tcgtagcaag gaatggaatc acaattaaac gacgccaggt tcctttatca
    32821 atcgctccaa cttttgggag gtggttggta tagagcacca aagtatgcgt tgggacatac
    32881 ttgaatgggt ctttgtattt cttctcagcc gcaatttcat ccgtcgagca gagctgctta
    32941 atgttagatg tgttgagtcg catcccctct tcaagctcgg ctgcgatgag taggcgtttc
    33001 cccttcgctt ccgcaagttc aggtttcaca ttgcgtcgga tttgacttgt aagaatatca
    33061 gctgagatgc tgccagagta attaccgagc acacggctga tgacattcca gaacgtggac
    33121 ttcccgttac gcccttcacc atacgagatg ataagcgctt caacgtaaac tttaccaata
    33181 gctgcaagtc ctacaatcat ctggacataa tcaatcaact cttggtcacc tacgaaaatg
    33241 gtgttcagag cgtccaacca aatctgctga ttggcgtcac tcggggcaca ttccgtttgc
    33301 ttcgtgatgt aatcttctga gttgtggtct tgtgatttgc ctgttcgcaa atcaaaggtt
    33361 gctgttggtg tgttcagcaa gaactcgttc acatctagta accgttgctc gatttcaagc
    33421 atgggctttg cctcggttaa tgctgacgag agatacttgc tgtcgcggcg tttgacagca
    33481 aacttcttat actcttgtgc gtgttggtac aaatcaaaac tatgacgttg cgctgtgttg
    33541 aacatggtga ctgccttttt cggaccaaca ctagcaagta catcgaacgc gccattacta
    33601 accatttcct tggtacgctt ctcaatttcg gtttcagatt cttccagttg acgttctgtc
    33661 aatgcgtgga tcacaccttg cgcttttggt ttcgactctt cccaatagct gccgttgtag
    33721 acaaggtaac cagttgacgg tgagtaatga agcttacatt catactcgcg agctaagacc
    33781 gttgcttgac ctaagtccga gtagtcggtt ggctcaagtg ataaatcaga gttgtactgc
    33841 tctggaggaa tgtagccttc ttgttgtttc actttcttcc caaagttgac cgcgctattc
    33901 cagatagctt taagctcctc ctccggcagt ggcggattac atttttggga ttgttcgaga
    33961 tacttgtgat acgtatcttc cgttgcaccg tagcgtttga tgagctttcc tgcgatgtga
    34021 ctcatgcttg aattacggct gccttcgcta atctctgact gcgcattatc ccaatcagca
    34081 aacagatcat cttcgagaaa atcagtgatc aacttactac cttcatagat ttcaacttca
    34141 gagtttggtg ttccgaaaag caaacgtgcc gcatccatcg cattgttgtc aaagtatggg
    34201 aactcacttt gaatctgctt cttgagttcc acatattcat ctttatcagt tgtggcagga
    34261 atcgggaaat atacgtgaaa acgtggacgt gctgatttgc cacctttgtc tttcatgttg
    34321 ttacgtgaat agctgaccgc aaacgtcaca tcaggaaact gcatcgccac ttcgaaaggg
    34381 gctacccaat catctggatt gtctgagtgg tcattgtcgc aatcaagtgg aatcacatca
    34441 gactggatga agttgtcacc tttgcgatag ttgcctttgt actcagccat cacatgatcc
    34501 agcttgaccg tggcaacaag tgtttcttta tctgtcacca cttgcttggt tgggaacaat
    34561 ccattcttgg aattacccac ctgattggat gtgaataacg tcatcttaag catttgagac
    34621 ctccttcatc tcatcatcga agtacctgat atgtttcttc caatactccg cttgttcaat
    34681 ttcaactgcc atgccttggg agatggtgtc acccaccacc cagacttcct cacatttgcc
    34741 catgagaaca agtgacatgt gcattgccac atcacgctcc gttgcgtcgt ccatgaattg
    34801 tggatacatc agatgtgccg caactggcat gtagccacgg tcaagagcaa tacggcagta
    34861 acgcttagtg tttttatggt tgacgacaac atttcctgag aacggcgagc agacatagac
    34921 tgatgttctg taatctttac gtctaggcat tattatcacc tcgctcaatc actggtaaga
    34981 tgttcttctt cgatttcagc aagtcgtaca agaacaaacg acctttctgc gtccagtagg
    35041 tatgcatcac gctgcgctct gcatctatcg catgagtttt tgattgtgtg tagccttgat
    35101 tggcatactt ctgataaagc aaccacatct tgccttgctt gtactgaatt cccagttcat
    35161 gaagcaactt gttcattgcc tgtggtgaca taccgtagtc cttcgagatt ttagaaatcg
    35221 acatcacaga attgttttga agaatcaaat cgtagtagct aacttttggt ttcatctcca
    35281 aaatcatttg ttgttgcatc gccaccattt cttttttctc ttgtagctgc ttcaacactg
    35341 aaatggccca tgctggatct tcgattgact ttgtgatgaa atcttctgtc gcgtacacac
    35401 cgtgttggcg gatagacggc aagacctcat ttgtcaccca acgcttgaat ttcttcgcgt
    35461 tcggcatctt gctagaaatg atgagtgagt acaatccact ttcgttgata aggatagcca
    35521 ttgtcccatt aacgctgaac gattcgttca ccgtcttatc ttcctcatca acatgatctc
    35581 gtaatgcttt ttgtggattt gtgtagccaa gaattgatgc tacatcttta ccaacaaagt
    35641 gagggacatc gccaatcatc accgtgcgta cagagccaaa ctccgcattt tcaaaaactt
    35701 tcaattcgtt catcttgtga acctcctgta atagaatttt caggagaggc attttgcccc
    35761 ttctaactta caggcgaaga aaatggtcga aagtaaactc tgactcgaat ttatttttga
    35821 tttttttgcc ttcgtctata agcgaagaaa gaacccaatt cgaaccttat ttttgataaa
    35881 aaaatttgaa aaaaatatgg agccatttct gactccacat tgactaatcc ttttgataga
    35941 attcacactc gtatccatca gcgttcagta gcaagccttc agcccactta ggcgtttgac
    36001 tcatcaactg acagatgtct tcaaccttcg attccattgg cacttcgata atcacttcgt
    36061 cgtggacgtg cccgacaatg cgatacttac gaagattctc cattgcgaaa cagagaatgt
    36121 ctctagctat tgcctgcacg atgttttcga caaacttggg accatagctt tcaagacgtt
    36181 cccatttctt ggtagcaccg acaccttcat aggtaacagc ttcaccgccg aatttgttct
    36241 caccaatctt tggttttaca taaactaatt tacgtcctga caagagttgg atgaacagaa
    36301 aacccgactt gtattcaaac ttgatgccat gcgtcgctgt gctccgtcgt tctttcacac
    36361 aagtctttac tgccttatca acagcccacc aaagctccgt tatgttaatg tttgagtctc
    36421 gccaagaatt aacaagcggc tgtaattcat cctcagttag tcccatttca agagcatcca
    36481 ttgctttcaa tgcacccacc gatcctccgt accctagagc aagctcagca attttccctt
    36541 tttgacgtag atgcccattc acgccatgct tttcaactgg cacaccgaac atctgactgg
    36601 cagacgcaca gtaaatatcg ccaccatttt tgaatacttc ttgacgccac ttctcacccg
    36661 acagccaagc aatcacacgt gcttcaatcg ctgagaagtc agatacgata aatttcatcc
    36721 cttcacgtgg tacaaacgct gtccggagga gttctgagag tacgttcggg acattgccgt
    36781 agagaatgtt aagcatttca aaattgcttt gttttactaa actacgcgct tcagataaat
    36841 ctgacatatg gttctgaggc aagttttgaa gctggatgcc acgtcctgca aagcgtccgg
    36901 tacggttggc tccatagaat tggaataaac cacgagaacg attatcagaa cacgcaaagt
    36961 tttgcatggc ttgatacttt ttcacggatg acttggcgag ttgctgacgt aagaccagga
    37021 cttgctttaa attttcatca ataggttgtt cgagtagttc ggccactgcc tttttaccga
    37081 gtgactcggt ttcaagtccg tggtctagta accatccccg catttgaagc acgctgtttg
    37141 gattttctag tccagttaat tcttgcaatt gttgattcaa tccagctttt gataattcat
    37201 ccaactgaac ggcagcttcc acaaagtcct tatcaatctc aattcctcgg tcgttgattt
    37261 cctgatccag ccagtattct ttccaaacag aatcaagcac tggaaaattg gaaagttttc
    37321 cttggatagc tatttcaact tccacatcac gactattgta tgtcttaaat gctgaccatt
    37381 tagttggcgc atggtgtggt aagttcctcg tgcgtccacc attaactttg gtcggattgc
    37441 atggcacgca aaagtatcgg atgaggtctt ttccttctgt gagcttttgt ttatccaaac
    37501 caagaacagc accaactcct acaagtgaca acggcaaacc taatgtcgca ccccacacca
    37561 ttgtgcattg ccaccctcgt ggattcaaaa agccatccaa ccccagaaag cgtgacaaac
    37621 acacacgttc aaaactcgca ttgaaggcgt gtttaatcac tgtttcatct gatagcgaag
    37681 ttaatatttc agccgggata gtttcgtcca tcttcagatc aaccacttcg acgggaccac
    37741 catctacact gtatgcaaat agcagtattt caaaatcggg ggactctgaa tatttgtaga
    37801 caccacattt cgataaatcg acactactat acgtttccaa atccaatgac aaatgtttca
    37861 ttttcttcct ccaatcaaac aggcgacagc acttctgcca tggccgtttt tgttttaatg
    37921 attaagccaa gaaatcatca tccgccaagg tttcaaactc atcgcttgca gatacttgtg
    37981 agccgagtgg ttcgccgtca cgtacaaact ggatattccc aagccctgcc gctaccccgc
    38041 gatttccatt tgcgttaaag gcatagaaat taatcgacac acgagcgtag caaccactat
    38101 acacttgtga acgatccaag attggttgaa cgtgctgatc cacgatttgt ggtgcagtgg
    38161 ttgagttcgc attaatgaag taatggcctt ggtatgcttc atcgtcgcgt tcgatatccc
    38221 catcacgtaa tggcagcttg atttctgctt tgtttggttt cttcccaccg aatttcgcaa
    38281 gtccctcttc gattgctgca tcgattgctt tttcaatggt atcaatcgtt ctcttatctg
    38341 attttggaat caagactgct gttgaatatt tctctgcgcc cccgttgatg gatgctggtt
    38401 cccaaccgtg gaagtatgag agacgtgtgt ttgtaccagt gattactttg cttgttttag
    38461 acatattatt gttcctccgt taatggttta aattcttcgt ttaagttgtt tactgtgacc
    38521 gcttgtcgtt tatctgaacc atcgactagc gtcagcttgc cttttggttt gatgatgtat
    38581 tcgcccaaga tttcattgaa tttctcttta ccgagtgcct tcgtcattgc tgtgacacca
    38641 agtaactttt tatcatatgg gtcgtatcca gccttgatga ccgccgcagc gacatcattg
    38701 tcattagaat acttacgagt agagcgaccc tcgactaatt tgtgatgagt ccattgtttg
    38761 ccaccgagtg caagtttcaa agcacagtcc ttgatatcgt tactccaaga aaccaggtca
    38821 tccactttac cgatgatttc ttcgatttca tcatcactaa gaagctctgg tggtttgaac
    38881 tcatattgcg ccagtttcaa gttatggtct gctcgtgcac gacagctatt cttcgccgga
    38941 cagaatccac accatttccc gcattggtac tcaccctcac ctttgaaagc taaatctgcg
    39001 gcaggcgaca acacatcttt tgcccactga agtaattctt ctttcagcag tgtgtacgta
    39061 ctcacattct cacgccttgg ctggaaaatg gtcatcttga cttcttgaat gtcataaata
    39121 acatcgaaaa gctccaacgc tcccaaggca taacacatca tctgtgggtt ctgatacgca
    39181 tctaccaaaa ttcctagacc gtatttcatg tcaataacag atagggttcc atccgcaatg
    39241 acgagacagt cgccggtccc aaagccgtct gggacgtacc ttgagaagtc gagccgctgt
    39301 tctactatta caatagagtc aactgttgtc tgtttcgcct tagcgacttc ttccatgacg
    39361 aaggtcgcat aactttcagc acactgctcc atctcctcgt cgtagaattc aagcgtagat
    39421 gttgggtctg tgacgtcatt accaatgaac ttattcagtt tgtactcaca taaggtatga
    39481 gcatcagtgc cttgttgggc atacgggctc gacttgtttt caaagtgctc tgacaagcga
    39541 acacttggcg gacagtgaat ccaccgatta cttgaagatg cggataggac tgcgtgttgt
    39601 gttggcatct acaatccctc cgcatctgca accaaagccg cgtagttttc tgtcggcaca
    39661 tcactcaagc gactggcacc atatttctca atcagtgctt tcacttcagt tgacttacct
    39721 tcttgagatt tacgtgccaa caaaccacgg acttcttcaa gcgtcggttg tttgacttct
    39781 gattttgttt ccattgtcgg ttctggtgtt tcaacttgtg gttcaggttc attggttttc
    39841 atcgccacag ccaactcttg taagctatca gctaacgctt ggacgtcctc aacgacctga
    39901 agaaatagtt ttgttttact cacgtcatta cgctcccttc ttgttaactt cacggatttc
    39961 gactgattcg attgtgtccc ctggtgtgag cacgagaatt ttgtccatgt tgcccttacc
    40021 gaacagccac cgtgcaaacc agcgaggcaa atgacgcaca ccaccttgta ggacagtttc
    40081 tttgttaccc gtgtcatcag tcacgtttac tcggattttg tgttttaggt tcatgatggt
    40141 cctccttcat ctttatttgt ttaggatcta ctcccttaca caatacaggc gaaggaaaac
    40201 ggggaaagta aactcccccg taactttttt ctaaaataat tctttgatct tgtctttgat
    40261 gattccaaag gtgcgattga ttgactgttt tttcttgtca agcttgtccg ccacttcagt
    40321 ctgagacatg ttttcagtga cgagcatgtg atagatttct tggtggtacg gtaataattg
    40381 cgtcacgact tcacgcaaac gatcgatacg aggatcttct tcattatctt ggtaagcatc
    40441 gtaaaccaat gattggatgg ggttgtcccc ctcatcgcat tcatcatcaa attcattgag
    40501 cgacaaattg tagttacgtt tgaagtaatc gttggcttca ccatccacgt attcgtccat
    40561 tggataatag ccgtgctcat tattaaagtc ttcacggaac ttctctttcc aagccttgat
    40621 cttttctttt tcttcttctg tgcgttcagg acgtgcgttt ttataaaagt tgtaaatcgc
    40681 acggtcgtca gcgcgatgca gctcctgaat gttaagctca gtgacgccgt cttcgcctgg
    40741 gcgaatctca accacttctt tttggatacg tccattggca tcagtacgct caaacacgta
    40801 cttgtaagtg ctgcggtcgt tttggtgtgt tttcataaat ctactcatta gatccctctt
    40861 tcttgcctga aagagctttt gagcaaggaa aacaaacagg gaccgtgcca atacgtacac
    40921 cgtccctcat cacgaaaaaa gagcgcaata aagtaaggca tacaatatct gaaaaatttt
    40981 ctggataacc agttcttttc agtatttgta accttgccct attgcaactc aggcattgtt
    41041 atattttatt agtaggacta ttcgctttta agataaattc gttcactttc ttgaaacata
    41101 atgtagtgtt tatgaaccat aaatgataaa attaaatagt taaactacta tgttttaatt
    41161 atagaaaaag gcctcccttg ttttcgtcag tcatcggcgg caaacggcgg tcaacggcgg
    41221 acgtaacaaa ggaggttgta ttttggaaat aagtgaaatt actcaaacgc tttatccgtt
    41281 tatgggaagc gagtacgtga agccacagga ttttttaatt gctttactgg atagtgtttc
    41341 aaatgatgac gaggccacca ttttcggttt ttctgatgat aacctacgaa gattttacaa
    41401 tggtactcga ccattaacac aagagtatgc agttcgggtt gctggaaaat tcaacatcga
    41461 caaatgcacg acttttattt atgaacgtct tatagatgat gctccaaccg tccttaagca
    41521 acaattgatt gataaaggtg tggtaatccc caaagataca gacgtttctg aaatagtggc
    41581 ccatctttta cttgatgaaa tcacctcaat tgcttctcaa acaaaagtaa aaacaaaaaa
    41641 ggccaagccg aagcttagcg aattagagct cgcaagttta agaatcaaca ctaatggtga
    41701 tttctcttat aatgatgaag tgaaaaaatt ctttgacgat ttgccagttc cccttgatat
    41761 tagagctgag gagatgacgt atgtcatagc tcttttttca gcatatgcag atgccgatgg
    41821 atacgaagta tacgagcata caaacgacct gccaaattcg cggctaaagg atttcaagag
    41881 acagcgaaga aattactata aagctgaaag catacgtcgt ggggtccgtg ataactttac
    41941 acaagaggaa ggaactgagc actttgaagc tttaaaagaa gatatgtacg acggcatcga
    42001 ggaagtctat gaagaagact acgatgatgg cgttgagaga ctcaaagccg ttctacagca
    42061 gtcgtctgtt ataacattaa acggatcaat cttatcgttt ataccgggat tgctgcaaaa
    42121 ttctgctaaa aaaggcattt gccacatgct ggttaatgac ggaaagatca aatgggtgac
    42181 tgaagatgaa tagtttgttt aatactccat ttgaaatttc ggttcgagta atgttactgc
    42241 tctctgaatt taaagagcct gtcagcgctg atagaatttt aatcacagac ttcatcacca
    42301 cgtatggtcg cgactttgaa atttccgatt acaacttaaa tggtgataac agctatagat
    42361 tcagtgaatt tattgcgcga cgtgaattag ttaaagatgc agtcaaaaat cttgtactgc
    42421 aacatgttat ccaaccatcg caaagcaaag aaggttttaa gtatgcttta tcagaagatg
    42481 gcctaaagtt gatatcacaa ttcgtatcgg tatatgcaga aaaatactta caaatagctg
    42541 acgaatctgt taaatacatt caagataaaa gtgacgttga gcttctgcga caaataaacc
    42601 aacgcgcaaa ggaatttaaa ggagagcaat gataatgcaa aattattata ttgaacgtgt
    42661 taccataaaa ggcaaagatc gtgaagatgc aactgtcaag ttcaccaatg gactaaacat
    42721 aatttctggc ccttcaaata ccggaaaaac tagcattgtt acggcaatcg attttctcta
    42781 tggcgcgaaa aaagacgctg ctcccttttc aaccgatgca accggataca gtaaagttga
    42841 actgattatt caatcgacta atggcaaata ttcattaact agagaatttg atagcaactc
    42901 cattttggta tccaaaataa cgagttcagg tgaatatgta gattatgaaa aatataatgc
    42961 acgatctggg aaaaatccaa ttagccattt ttggttacaa cttttaggca tcgatgatga
    43021 ttatcagatt atcaagaatg aggatttcaa acgtcaaaaa ttaacttggc ggacattagc
    43081 gcctagcaca cttattccac aagatagagt caataccagc gcctctgttc tttttcctgg
    43141 ggaaacaacc caacgcacag cttttttatc atcgttgtta cttctgctat acggttcaga
    43201 ttttgcgaac tcggaagaaa acgaaacaaa agaacataaa aaaattagaa ggaactcggt
    43261 gattaactac atcaattcaa gcctttctga attatatgaa aactacgaag tggctaaatc
    43321 agaattacct gaggctccct ctgatgattt tcaacaaatt gtcgatggaa tgttcgctga
    43381 acttgaaaca attgaaaagc aaatcaatga tgcgttagcc agcagcaaaa tcttgataaa
    43441 agagatttat caaaaggaac agtatcttca tgaaactcaa cttcttttga ataggtacaa
    43501 acagttacat tatcaatatc aatcggatat tgaacgacta acactaatcg tagatggtga
    43561 gcgattatta aatgatcata atgaacatga tacttcacat agctgtccat tctgtaacgg
    43621 cgaactaagt aaacctgtcc aaacaaccta catcgatact gctaggtcag aattacattc
    43681 attgctctct caattatcag atttgacgga agaatctcat gataccgaag ttgaactgca
    43741 agagttaatg gaacagcttg atgagcttaa aacaagaaag aaatcgcttg atgcagcaat
    43801 caacgatgaa ctaaaaccga aagctgagac gttaaaggat cgaatcaata aaatcgaaac
    43861 ttacagggat attaaacaac agctaatctc gatggaaaaa atgcaaaata agcttaaaag
    43921 tgatcttgaa aaaatcgaga atgagccaga agacgaacgt atcctttata aacctaaaga
    43981 gaaatttgat gcagaattct acaaagaaat gtctgagtta ctggactcca tgttacgtaa
    44041 tatgcattat aaacctaatg aatttcgtaa cgcatttttc gccaaggata ttttcgacat
    44101 caaaatcaat ggaaatctaa aatggaccga aaacggacat ggatatacag cgtttatcaa
    44161 ttcagtagtg gtaatggcat atcgacatct cttgtataat cacgctaagt acacaccttc
    44221 tgtgttcatt atcgattccc ctcttcttgg attagatgaa cgcaaaaaac aagatggcga
    44281 tgacaatatg aaagaaggac ttttcaaata ctttatggat catcaaaacg aaggacaact
    44341 tatcatcgtt gagaatacaa cgaacttgcc agcactaaac tatgatgata aacaagtaaa
    44401 tcagattgtc tttactgaag ataaaaacaa tggacgctat ggatatctat acgatattta
    44461 taactagaac aggaatgatt acatgaagtt tagctacaat ccgctttgga aactcctaat
    44521 cgacagagga ctgaataaag cacaactcag aaaaatgact gatatcagtt cagcaacact
    44581 ctcaaaacta aataaaggcg aaaatgtaac aacagatatc ttacttcgca tctgtgaagc
    44641 acttgattgt gatttatcgg atattgttga aatcgttcgc gattaaatat caaaaatgca
    44701 ctgtattcga ttcatgagta cagtgcattt tcatatttat ttttgtaatc cagcgattac
    44761 ttcattgatt ttcgattcaa tatcattcaa tggaaccata taccattcag tagctttttt
    44821 gaagtttcca tcctgaaaga caacttgttt gtcctggaat acatgatgga gagtgcgctc
    44881 aagtagctga gcgttgacat tttgtacttc agcagaaagc atgaccttca ctggtgcatt
    44941 taagtaggtc acgtcgtttt ccgcattggc aattctactt tcaacatcat ttcgagtgaa
    45001 accaatctta tacaggttat tccacttgct aatatccgga ttcgtgctta atgacttcag
    45061 cacgtagata taacctgtcg taaactcttc tccaaagctt tcagccagtg tttcctcatc
    45121 taccggctct gtcaccatcc ttccatgacg ttcagtcgag tatagagacg aagctaaaga
    45181 ttgtaacaac agattcttat tctctgtccc attctcataa acaacatgca ttctggcatt
    45241 tgttttccca ttatcagcga taaaggtatc cttcgaaatg gaaacgatat acatcattac
    45301 gccgttttgg atgtagaatc tattttcatg aatatcatac tgctcaaacg ggataatttt
    45361 tcgtctgcca gatgcgatat ctgcatgcac ttgatcgaac aagctcttat acttttcaaa
    45421 atgacttgca tgttttctgc ggccaatttt atctgcagcg ttcacagttc tacgatagcg
    45481 ggaaacatcc aacaagtctg aaaaatcatc aatcatatca tctgagccta agatatcatc
    45541 taaattcaac tcgtcatcga tttcaactga atcagcgaga aactctccat ccggttcgaa
    45601 ttctttctcc aataaaccaa gagtgtcata cttcttaacc ttctcagacc tttcgggttt
    45661 tcctcgaaat ccgctaagac gagcccacaa tgcgcgttca ctagaccatt caggagtttt
    45721 ttctggctct cgtccgtttt cattcacaaa gtctataatc tcctgaaatt tagcaacttc
    45781 tgggtcttgt tttacaactt tctttttttc aacttgcttg gttaaatcat caaaagcgtc
    45841 atcttcaaag atgctatcta aactattcgc catcttcttt cgctgcctct ctttcacgtt
    45901 ttatctcagc aagtcgtagc aaagcaaagg ccaagtattt ttcaccatca tctttactat
    45961 ttcgatcagg atttcgacca ttttctgcac ggaacttctt agcagcttgg taggccgcaa
    46021 ccaattgttc gtcagtaaaa tcatacttat ttgatgacaa ataatcctga ataaaccgaa
    46081 gagtcggtgc atcaatatca cgactaatca cttcatacgc acgttggaac ggattcactt
    46141 gatcgattaa atcaatattc aattcatcaa tgttaatgaa acgatcggcc atcttaataa
    46201 tttcgcggct accatcttct gaagttgttg tccgtccagc cgcaatattt atatcggcaa
    46261 ctgtatgttg acgaacggtt tctaactctt cgttcgtcaa atcaggataa cgatcaatga
    46321 tgacacgtgg aatcaacgtc ttattaatca tcttcgcatc agcacctgaa acaatcgcat
    46381 tttgaatatc ttggctctgt aaaatgctag atttcaaatc ttccatatca ttttcgatga
    46441 ttttctttgt acgttccgtc gtatcagcag gtttcagtcc tctgattgaa agatcacctg
    46501 gtttgtctgg cttaccatct tttggtttaa acttgtaaac tggcgccaac acttgttcca
    46561 taagtagaga tgcagaaatt gccttcataa tactgttaac cgcgaataat acttcgtcat
    46621 cctgtgcatc tggctgcgca ataagatttg taaactgtgc atgagtctta ttggggctgt
    46681 cacgagttac gcgaccaatg atttgaatca cttcagttaa tgagttacga taaccgattg
    46741 tcagtgcata ttccgcaaat ggccaatcga agccttcttt tgccattcca agtgcaatga
    46801 tgatatcaag cttatcaaga tctttaatgt catccgtaat acttgctaga tatgctgaga
    46861 ctttttcacg tccttcttca ttcacaaggt cggccactaa tagtttttta ccatctggac
    46921 gttcgatttc ataaatacca tcctcgttct gtgtgatatt accaccgtcc gtcaataaat
    46981 caagtatttc atcaacttcc gcgtatttat ctttagtaga ctcaccagaa ttaacattag
    47041 gaatatggat aattgatttt ttgttgacat caattacttt atccaaagca tctgtatatt
    47101 ttccacgata gaaactatac cccattgcaa aggatttcag atatttatat ccttccagtt
    47161 gctcataata agtgtaagtg accttatcaa atttatcttc gtcggctggt tccagaatct
    47221 gaacagagtc gccacggaaa tatgaaccag tcattgctaa gatatgcgca ttagagtttg
    47281 acatgatgct acgtaaggca ttacctaaca cagatttatc atcacggctc acatgatgga
    47341 actcatcaat cgctaatagc gtattgtcaa ataatgaatc gtccaactct tcaaaagcaa
    47401 atcgcaacgt agcatgtgtg gcaatcagga tattatcact tgggtcagtc gagttcatga
    47461 atttaacaaa gtgttgaacc tttgattttt caccgccccc aatagttaag ttgtcacttg
    47521 gctttacttc ccaatcccaa taaaaaccaa acttcgataa ttcagtatct ttaaatgatt
    47581 taccaattga gcgttcagga actgcaataa ccactttttt aattccttga ttatggagtt
    47641 tatccaagcc aacaaacatc aatgcacgtg attttcctga agctggtggt gattttacca
    47701 acaaatactg tgacgcacgc ttttcataaa cacgttcttg catttcacgc ataccgaggt
    47761 catttgttgt ttttgattga tttttatcaa aattaatttc aaataaatta ctagtcattg
    47821 attgtcatct cctgatagag ttttagtaat gttccaagtc gatcttcgtc agattcaaac
    47881 gggcgttgct gataagctct atcaacaatc ccatccagtt cctggtgttt cttacgtaat
    47941 gattctggca tactgtcttt attatacaaa tcggctaacg tgccgccttc gtattctcgt
    48001 aaatcaagaa tttctgtcat cacgcgagtc atttcatttt tacgctgggc ggataaatcc
    48061 tgaatcggaa aagtattata gactaacccg gctgaataac ggtaccgagt ttctaatttc
    48121 ccaccgacag agcgcagcca caccatatgc attcgggatt ctaacagacc aagcagccac
    48181 attggagcgt tatagattgc catagccgaa tctgaaatta tagtatcgct cccaactata
    48241 cccataggaa cataatctct tttttctgat gacacacgag gaattagaat actattctca
    48301 ctggtttcat cataacgaac ttcaccaaat gaccatggct tctcagctag tgctcgagtt
    48361 tcttttctct tgcttttttc cctataactt tttacatttt ttattgtttc tttaataaga
    48421 gaattttgtt tgtatctagt tgcatcttcc tcggtcaacc ataaagtata tcttaagtct
    48481 ccatttatta atcctgttga tcctaaatat ttcttaaata aatgtttaat atctggatat
    48541 ttttgaatca aattccagta ttctgccact gaaaaaacta gattaccacc atcatttgcc
    48601 atactgccaa atttcaaaga gggtaaattt gaaatactat ttcttgattc gttgacaaat
    48661 acattttcac cattagctaa atatgcatta atatttgaaa cactcattac tgacccattc
    48721 tctaaatata acttaggatg aatattcaca ttatttgtca taccaataat gacaacagtc
    48781 acaccggcat tattctttgc attgttgttc cacttaaagc tctgatatgc aaatgaaatt
    48841 cttacctcat cttttaaaag gtacggccaa aggatactaa cttgttcacc ttgacaaatt
    48901 gaattagtag taacaaatgc gaacgagcca tgagatgttg caatatattg tttagctttc
    48961 caaaaccacc caaaaatata atcaagcaat ttatattttc gaatatcgcc aacaagtttt
    49021 tccatatctt cacgttgttc agcattttga agacttttgc ccagatacgg tggatttccc
    49081 attatatata tttcatgatt ttctttgagt cgcaacacct cattccaatc aattcgaaga
    49141 gaattggcac acactatatt cccagcatct tttaacggta acaactcagc aatggtacct
    49201 ggtattgctt cttcaagctc tttattcatt tgatgttcag caatccataa tgaaagtctt
    49261 gctacttcat gcgcaaaatc atccaattca atcccattaa aattaccaag atgaatcact
    49321 gatagtggca tggaatcatt attttgtaag tcgcccaaca attgcaaaat cttaatttca
    49381 agtctacgga tttccttata agcaatgatc agaaaattac cagaaccact ggcgggatcc
    49441 aaaaacttaa tattagaaat tcgcgtcaac aaagcactta gatcattgat gattgttctc
    49501 ttattatcgt ttcttgtttt ttcagtaatg tccttcagct cgttttcttc actgcgaagc
    49561 tttaaagttt caaatgactc tgtcaaatca tctaagaata acggcttgat gactttcatg
    49621 atattcggca cgcttgtata atgcataccg gcaacgtgac ggtcttctgc tgaagcaact
    49681 gattgaatca ttgcgccaag gatatcagga ttgatttcat tccaattcaa taattcccct
    49741 gcttcaataa gcagctctct tgaaactttt gaaaaggtca aatccacatg tggctcacca
    49801 aacagttttc cattaacata tggaaattca agtagccact catccttgcc attgcgagct
    49861 aactcattaa tatccaagat tgcaaagagt tctttgatta cttcgttaaa attcgaacca
    49921 tcttcagccg ttcttgtttt tagaacattt gtaaaggaac ccttattcat aattcctgta
    49981 tcttctgcaa acaacaagaa cagcactcga atcaagaaca agttaaatgc atgctcgttg
    50041 gcatcaggat tatctttagc caccacatca tacaattttg caaaacgttc agcagctttt
    50101 cggtcagctg gattttcagc ttgataatca gccttctcaa tgccattcca cgctaagaaa
    50161 aagtcagcat tagccggtag ctcttcaatt ggaatattca aagtttcatg agttttcgta
    50221 tcaattgcag caatatctaa ataatcgttt accaagatat atcgtggttt agatttttgc
    50281 tctttgattg attgagcaat cgcatcaatc gcaatgacaa cgttgccttc aatttcttca
    50341 taatgaagtt tgttcttaat cacaaatggt tcaccattat cgaattttga ttttgcacgt
    50401 gtaatcgacg ttttggggat atcgtacaag gacaaaaatt gagtgatgaa atcatcgccc
    50461 ctatttgaaa gtattttttt tacaccatct tcaatttcag tgatgtttaa aactttattt
    50521 tttgccattt atttaacatc ctccgcatct ataacatctg aactaattat ttcactaaca
    50581 tcaacattta aggcagcagc aattttcaat aatgactcca ttgcaaccaa ctcattttta
    50641 ccgagttttg ctattacatt gggactgacg ccagatattt cacgaagttg agttttgttc
    50701 atattcaaat caatcaataa cttccacaat ttattataag aaattgacat actacacctc
    50761 tattctactg gtatcttcaa ttatatcatc tatgagctat cattattata cgaaacatga
    50821 aatattttcg cgaacgcaaa ctatttttct aaaataaaag ccttcagcat agatttacta
    50881 aaggcgagat aacaaatatt aattccactt tattttaacc ggaatcccca attgacgagc
    50941 tagtttatca attttgattc tgacattctt tttgttgaaa tttgcataaa cccatactcc
    51001 atcagctacc ctggctgaat ttggtcttcc gttatacatg gatggtttac gtcctaaaat
    51061 tatagcgaac gacggcgtct catcgacaat taattcttgc agttgattgt tgtagtttcc
    51121 atccgcaaat agctgagaaa caaaatcaac caataaacct ctagcagatt tcgaagatat
    51181 ctttttccca tcatactcaa tttcatattg tccagatgaa ctaaattcat aaatttgggt
    51241 ttcactgtct tcggaagtac caaacaaatc gatatttgca atttcatcta taatttcaaa
    51301 cactgtgtca tatagctgat ccgattttgc tttttgaagt ttatcaatat acgaagtatt
    51361 accagcagtt gaattcatca tttcatagtc agatttttct tggaaatctt ggataagtcg
    51421 tttttcaaga taatctgtat ctgcttttga cattttccca tctgctcttg taaagaataa
    51481 aactgattca acccaagctt tatattcctt gagaaagtgt tgagataaac gaagtgaaat
    51541 tgattgccct gctgcctgac ccacgtaacg cttattacca ccaatcaaaa cataaacagc
    51601 tggataattt gaaaaatcct gttccttcaa atacggcaaa tcactacgac tcattgaaac
    51661 caagattgct ggctcgcgtt caaaataaga aatattatct ttctgtgtat ctgaaaatat
    51721 aaaaatttgc ttcataagtt tgtctccctt gaaaactcga taatatatta tatcacgtaa
    51781 ccgttatcaa aatcatctaa cctatcaagt ctacggtgta actcctatca actcaaagct
    51841 ccatagcatt tccatcaact ctacgctcac ccaaaaatcg tcatttcaac acgaaaaaag
    51901 agcaatccgg aaaccatccc gaatcgctct aatccttgtc atatcaacgt tccacacgtg
    51961 taagcagtcc tacacactcg atgtgatggg tttgcgggaa aagatctaca ggctgaactt
    52021 tcttcacggc ataacctgct tctgcaaata acttagcatc tcttgcaaaa gttgccggat
    52081 tacatgagat atagacgatt cgttctggtg acatttcgat tgctgattca ataaaatgat
    52141 gatctaatcc ttttcgtgga gggtcaacta cgatcacaga tggtttgatc cctttttctt
    52201 gccatttttt cataactttt tctgcttttc cgacttcata atgaacattt tcgattttat
    52261 tatttaaagc attgaactgt gcatcttcaa ttgcttctgg aataacttcc atcccataca
    52321 ccttatccac tctgtctgct aaagacaagc caatcgtacc tattcctgaa taagcgtcga
    52381 tcacaatgtc ttttttatct aactcggcaa actcgatggc tgtttggtac agatactctg
    52441 cttgttgagt atttacttga taaaatgact ttgctgagat tcgataggtt tttcctaata
    52501 gctgatcttc gatatagtct ctgccatata aaactttttc tttgtctcct aaaatcacat
    52561 ttgttttttc ttcattgata ttttggatga ccgaaaccac ttctggcaat tcttcatgaa
    52621 tagcttcagc aatttccttg gatttaaaga atttttcttt acgagtaact aaaacgacca
    52681 tcatttcatg ggaataatga ccacgacgga taatgatgtg acgaagctgt ccttcatttt
    52741 tttcttcatt atatcctcgt acttggaacc gttgcaagat atctctgact actttgattg
    52801 cttgatcgat gacaggatct tggatataaa aatcttcgat agggactaga tcatggctat
    52861 tctttttgta aaaaccggtg gctaactgtc catcgatacg gcgaacaggg atctgtgctt
    52921 tattacggta gccgaatggt tctgccattc cgaccgttgg aagtacagga acttctggca
    52981 tcttagcaat cttcgccatt acatgctcta cttgtttttg tttgaagaca agctgttgtt
    53041 catacttcaa atgggctaaa ggtgcgatac ctatgcgaag taatgcattg ttttctacag
    53101 gctgacgatc aggtgattgt gtaataaatg tttcgacttt tccatatcca aacttattgc
    53161 ctgcttttac tactagtatt tctacttctt ctccagggag tgcattttcg ataaataatg
    53221 gataaccatc gatttttgct acccccattc cttcataact caagtcgacg atttcgactg
    53281 tatatcgttc gtttttcttt actatttgct gttctgtcat attttgctcc tttaagccta
    53341 tttttcaatc ctactgaact ctactataaa tcagctaatt acacaactaa aaccggcact
    53401 gaccttacta cgttcagcac cggcttttga ttaattcttt tgattaggtg tttcgtctgc
    53461 tatttttccg tcaccgtcga tatcttcatc cgttaaacgt tcgacttctt ttacgaattc
    53521 tttgctgaca acctctaatt cttcttcatc tgtacctgtg attgcttcat caggaatctg
    53581 atcggtattg gcaaagaact caatgtgttg atgaagattt tggaaatgca tcggtgcttc
    53641 cccgccgtat tcaccatcta agttgatcat tactggtcga ttgatatcat cttctgtttc
    53701 aacgaacaaa ctgcttgttt tggtatagat tattcttgga tcatcgacat gtcgaccgcc
    53761 attcagcatg agtgctgcaa tatgcaagat ctcaaagata tttgctgtct tcacaatgat
    53821 taaagaaaac ttcccatcat ccaatttggc atctggcgcg atctgttcga atcctccaac
    53881 agagttcgtc agccctagga aaaacatcga agcatttccg atatattccc cttcatcata
    53941 cttcaagcgc attttgattg gctttactcg agggagcatc tctgctcctt tcgcaagata
    54001 agcaagataa ccaaaaatac tctttagttc tgatggaact tcataagtca attctgtcaa
    54061 atagccacct gcagcgatat tgatgaagta actgttatca gaaaggccga tgtccatttt
    54121 tacggtttgg tgttttttta tcacttctgc tgctgcttta atattatctc taggaatttt
    54181 taatgctcga gcatagtcgt tcgttgtacc agctgggatg atggccattt taggacggtg
    54241 ttcaagcgga gcgattccat taaccacttc gttgattgtg ccatcgccac cagctgccac
    54301 tactagatca aaacctagtt ctgcgactct acatgcttca ttcttggctg aatctggttc
    54361 tggtgtagtt gcataggcgc tggcttcaaa tcctgcttct tctaatgcct gtaggatatc
    54421 cgccaaattt tttttcaata attcttttcc cgaggtgggg ttataaatta cgcgtgcttt
    54481 tttcattttc agcctctatc gtttcgctaa ttcatcttta agtagcttat tcacgacacc
    54541 aggatttgct tgtcctttag ttgctttcat gatttgaccg actaggaagc caaccgcacg
    54601 atcttttcca tttttgaaat catcgacaga ttgttggttg ttatccaaaa tttcattaat
    54661 gattggcagt agtttgttcg gatcagataa ttgcacccaa ccattttttt caactacttc
    54721 ttgggcttct ccgccatttg tgatcaattc acggaagact tttttggcga tttttgagct
    54781 gatcgttcca tcggcgatca aacgaatcat tcctgctaag ttctcaggtg tcaattttgt
    54841 ctcagctaat tccagtttct cactgttcaa gtaagcagaa acttcaccca tcaaccagtt
    54901 agatgcttgc ttggcatctg caccattttc caaaacagct tcaaaaaaat cagacatttc
    54961 ttttgtaagt gtcaatacca ttgcgtcata ctctggaagt ccaaactcac ggatatagcg
    55021 ttcgcgacga gaagctggca tctcaggcaa actagctttt actttttgaa tccattcgtc
    55081 atcgatcacg aatttcggga tatcaggctc tgggaagtaa cgataatcac tagacccttc
    55141 tttgacacgc atcaaaactg ttttattcgt tgtttcgtca aaacggcgtg tttcttgttg
    55201 gatttctcct cctgaaagaa gaacttttgc ttgacgtttt tcttcaaaag ctaaaccttt
    55261 tttcacgaaa gataaagagt tcaagttttt caattctgct ttcgtaccga actcttcttg
    55321 tccataaggg cgaagagaaa tattggcatc acagcgcata gagccttctt ccattttcac
    55381 atcgctgact tctgtaaatt ggataattga tcgcaatgct tctagataag cgtatgcttc
    55441 ttctggtgaa cgcatatcag cttcagaaac gatttcgatc aaaggcgtgc cttgtcggtt
    55501 caaatcaacg taagaataac cgccaatacc atgcatgttt tttccagcat cttcttctaa
    55561 atggacacgt tcgatccgga tttttttctt tttaccttct acttcgattt cgatccatcc
    55621 gtcatgaccg attggttgat caaattggga aatttgatat gcttttggat tatctgggta
    55681 aaagtaattt ttccggtcaa aatgagtatc tttagagatt tcacagttta atgccaaagc
    55741 tgctttcatc ccgaattcca gcgcagcttt attcattact ggcagcacgc ctggataacc
    55801 ccagtcgatc acgtttgtat tgctgtttgg ttctgcacca aaatgagctg gcgctggaga
    55861 gaaaattttt gagttggttt ttaactcgac gtggacttct agtccaatga ctgtttcaaa
    55921 attcattcgt ctttccctcc taaaatagct ggttgttgtt tatggaattc tgttgcttgt
    55981 tcaaacgcat atgccgcttg atacatcgtt ttttcatcaa aataattacc gatgatctgc
    56041 aatccaactg gtaatccttc tgataaacct gccggtacag acatacctgg aagtccagcc
    56101 aagttgacag ggatcgttaa gatgtcactc atgtacattg tgattggatc gttaatgttc
    56161 tcaccgattc cgaatgcaac tgttggtgaa gatggcccga tgatcaagtc ataatctgca
    56221 aagacttttt caaaatctcg tttgatcaat gtacggactt gtcccgcttt tttgaagtaa
    56281 gcgtcataat aaccagcact taaggagaat gtacctaaca tgatacggcg ttttacttct
    56341 tcaccgaatc cttctgaacg tgagttgaca tatacatcat ctaatgattt cacattttct
    56401 gagcgataac cgtagcggat accatcgaat cgttgcaagt tggaacttgc ttctgatgag
    56461 gcaatgatat aataaacagc cacaccgtat tttgaatgag gcaagctgac ttcttccaca
    56521 acagcaccta atgaacggaa tgtttctgct gctttcaata cagcttcttt tacgccaggg
    56581 gccacacctt cgcctaagta ttctttaggt aaagcaatct tcattccttt gatgtcgcct
    56641 gtcagtccct ctgcaaaatc aggaactgaa acgcctgaag aagtaccgtc tttttcgtca
    56701 taaccgctga tagctgtcaa tgctaatgca ttatctttca ccgtacgggt cattggtccg
    56761 atttggtcaa gagatgaagc aaaggcaatc aaaccaaaac gtgagacacg gccataagta
    56821 ggtttcatcc ctacgatgcc gttaaaagct gctggttggc ggatacttcc cccagtatca
    56881 ctacctaaag aaactggaat ttgtccagct gcaactgctg ctgcagaacc accagaagag
    56941 ccaccaggta cttttgttgg gtcccaagca ttttttgttt tcttgaaata agaagtttct
    57001 gtacttccac ccatagcaaa ttcatccatg tttagcttac caacagggat catatctgct
    57061 tgatagattt tatccatgac cgttgcatca taaattggtt caaaattgta aaggatctta
    57121 gatgcagcgg ttgtcaagat atcttttgtt acgatattat ccttgatacc aattggaata
    57181 ccagcaagga cgttgctttc atcaatccct ttttcatcta aagcttttgc ctgttccaat
    57241 gctttttctt cgttcaatgt aatgaatgct tcgacctcag gctctgtttc tttgattcgt
    57301 tcaaatgttg cttttgtcaa atcttgtaca gtgatttctt tcgatacaag catatgatgc
    57361 agttcttcta gagaacgatt gtgtaattct gtcattatgc gccagcctcc ccattatcga
    57421 tgattgcggg tactttgata aagccgtctt ctgtttccgg cacatttttc aataattcat
    57481 cgcggttcca tccagcttgc gcccgatctt gtcgcatcac gtttactgtt tcaaccacat
    57541 tcgacgtaaa tggaacacct tctgtatcta cttcttccag catttcaacc atatcgatga
    57601 ttttacctaa ttggtcggta aaacttgcaa gttcttcatc tgcgaacgca agttttgcta
    57661 atttggcaac gtgtttcact tgttcttcac taatcgccat actacccctt ctttctaaac
    57721 gagttaaaaa ctttgctctc tattttaaca caagagtgcc gtgtctgaaa ttgtttattg
    57781 aaatttgtct gaaaaaactc atttttcatt cttctactga gcgactttta gccaatgcat
    57841 ctgccatttg atcttctgtc caaacttcga ttcctaattc ttgtgctttg gtcagcttgc
    57901 tgccagcatc ttctcctgct accacgatat ccgttttttt cgatacgcta ccagtcactt
    57961 tcccgccaag attttcgatc gtctcttttg cttcttcacg agtaaatcgt gttagtttac
    58021 cagtaaggac tactgttttt tctttaaaag gagactcgac ttcttgcaat tgggcatttc
    58081 gaagcccttt gtattcgaaa ttgacgcctg cttgttttaa ttcattcatc aattcatgga
    58141 cttcttcgtt actgaaataa gtgactacac tgtcggcaat gatatcaccg atcgtatcaa
    58201 gcgcaataat cgactcatag tcacttttac tcaatgtttc tagatcaccg aaatgttctg
    58261 ctaaaatttt tgctgctttc gctccgacat gtcggattcc taacccgaaa atcaaccgct
    58321 cgaccgaatt atctttgctg ttgtcgattg ctgtcaaaat attatttgct gatttttctt
    58381 ttattttgtc caacgtcaat aactcttctt ctgtcaattt gtaaagatca gcaacatctg
    58441 ctactagttt tttatcatac atctgttcta gtacgcgcgg ccccaaccca tcgatattca
    58501 tcgcatttct tgaaacaaag tggttcaaac cttctttcat ttgagcaggg catttaggat
    58561 tgatgcaccg taaggctact tcttcatcca aatggacaag ttcacttcca caaactggac
    58621 aatgtgtagg caattggtat tcttcgctgt ctttaggtcg tttatctaac acgacttgcg
    58681 aaacttctgg gatgatgtct cccgctttat aaatcagaac tgtatctttc aagcggatat
    58741 ccttcaactt aatataatca ccgttatgca ggctggcacg gcttactgtc gttcctgcta
    58801 ctcggactgg tgtcatgata gctgttggcg ttactacccc tgttcgaccg actgtccatt
    58861 cgatgttttc gattagcgtt tcaacttctt ctggcggaaa cttgtaagct gctgcccatc
    58921 tcggtgcttt gacagtaaaa ccaagttgat cttgtagcga gaattcattc actttgatga
    58981 cgattccatc gatttcatag ggcaaatcca cacgtttgtc atgatattct tcaatgtatg
    59041 accacacttc atcaatcgaa tgacatagac gtttttcccg attcgtgtgg aatccaattt
    59101 tttctagctc ttctagcgca tcatactggg tttttgcttg cataggaccg aaatcagcta
    59161 ctgtgtataa aaaagtgtcc agatttcttt tcgctgtgat tttagaatct aactgacgca
    59221 aagaaccagc tgcggcgttc cgaggattcg caaagatatc ttttccctct gcttctctgt
    59281 cttggttcaa ttggacaaat gaccgttttg gcatgaaaca ttcgcctctt acttcgatgt
    59341 tcattggttc ttttaatttg attgggaccg accggacagt ttttaggttt tcagtgatgt
    59401 tttctcctac agttccatcc ccgcgagtcg cacctctcac gaaatttcca tcttcataac
    59461 gcaaagaaac ggaaagtcca tctattttta gttcacagca atagtcaact acacgtccca
    59521 ctgctttttg tacacgttga tcaaaagcta tcagctcttc tttgctgaaa acgtcgttca
    59581 aactgtaaag cggaatatca tgagtgactt tttcaaaacc ttctaatact tttccgccta
    59641 ctcgttgagt gggagaatca gacgtgatca aatcaggata ttgttcctca attgctacta
    59701 attctgcgta ttctttatca taaacatagt cttccactgt tggtttatct tctacgtagt
    59761 attcgcgaga ccattggttc agtcttgtac gaagttcttc tgctcgttta gcagcttcgt
    59821 caatcgtcat ttcatgttcc atcatttacc ctccactact aagctttttc aattggcgca
    59881 aaggcagcca aaagacgttt tactccttga cttgggaaag ccacatctag ttctagatcc
    59941 ttcgacgtcc cgcttactcg gacgactgtt cctagacccc attttttgtg tttgacttta
    60001 tctccaggtt tccagctgtt attttcccct ccagtagcac ttttagaggg aacaaccgct
    60061 gtctctggct gtttatagct atatgctgtt tttttagcga atacaggtgt ttttttaggc
    60121 gtaggacgca tgccttcgat ttccagtaac tcctgatcga tttcttctac aaaacgagaa
    60181 ggtcggttat attgcgtccg tccatataat gttctggaga aagcatttgt taaatacaac
    60241 gcttcttctg ctcgcgtgat ccccacatat gcgagacggc gttcttcttc cagttcgctt
    60301 tcttccatca atgctcgaga taaaggaaaa accccctctt ctagaccaat caggaataca
    60361 actgggaatt ctaatccttt ggctgcatgc aaagtcatca atgtcacttg ggaagcatct
    60421 tcttctaaat tgtcaatatc ggaaacaagt gccagatcat ttaagaagac cgttagtttt
    60481 tcttctggtg catcagcgtc ttcttcattt tgctgttcga attgtttgtc gaattcttgt
    60541 gtgaccgtta aaaattcctc caagttttcg atgcgtgctt gtgcttctaa tgtattttgg
    60601 atttttaagt cttctagata tccgctacga tccaaaacct ctttcgtcaa ttctgtcact
    60661 gtcaagtatg ggatcatctg agtgacttct tggatcatct ctccaaatgc acctaattgc
    60721 tgtcctgctt ttccaccgat atttgccaga tccacatttt gcgctgcttc aagcagtggc
    60781 cattcatgaa gagaagcaaa actgcgtagt ttttcgatag atcctggtcc gatgcctcgt
    60841 ttgggcgaat taacgatacg ctcaaaactg attgaatctt gaggatttgc cagcacattc
    60901 aaatacgcta aaatatcttt gatttcttta cgatcgtaga acttgtgccc tccaaccatt
    60961 ttataaggaa tatttgcttt caacagagtt tcttccatta cacgagattg tgcgttcgtc
    61021 cggtacaaga tggcaaagtc gccgtaattc cgatgattgc ttcgaatctc ttcttgcatc
    61081 cgatctacga taaaacgggt ttcgtctcgt tcattatctg cacggtagta agtgatcttg
    61141 tttccttctt tgttttctgt ccacaagttt tttggttttc gattgctatt gttttcaatg
    61201 acttggtttg ctgcgctaag gatgtttttc gtggagcgat agttttgttc tagcaaaatt
    61261 actgcagcat ctggataatc tttttcaaaa tccaagatgt tctgcatatc tgcaccgcgc
    61321 cagccgtaaa tactttgatc tgcatctccc acgacgcaca ggttcctaaa tctacctgcc
    61381 aataaattga ctagcgtata ctgtgcgtga tttgtatctt ggtactcatc tacatgaata
    61441 taatggaatt tgttttgata atatgtgagt gaatcaggat gttcttcaaa taatcgaatc
    61501 gtattcatga tcaggtcgtc aaagtccata cattgattat attgaagctc tttttggtaa
    61561 gcagcataac attttgccgc aatctcttcg taataactgc cttgcatttt tgcaaaatcc
    61621 tgaggggtct gcaggctgtt ttttgcttga gagattgttc ctaatatact tcgaggatca
    61681 tattttttcg gatcaatgtt cagttctttt agtatccgtt tcatcaaggt cagctgttca
    61741 gaagaatcga tgattgtaaa gttccggtta tacccgataa aatcaacatc tcttcttaaa
    61801 atacgcacac acatcgagtg aaaagtcgat acccagacat cttcgcctcc agatgcgaga
    61861 atcgcattga cacgttcttt catttctttt gcagccttat tggtaaaagt aatcgctagg
    61921 atgttccaag gattgacttc tttttcctca atcaaatagg caatgcggtg tgtcagcact
    61981 ctcgtctttc cacttcctgc acctgccatt agcagtaatg gtccgtcagt gtgaagaaca
    62041 gcttctttct gacgggggtt catcccgttt aataattcag ctttggatga cattaacaac
    62101 atcctttctt ttatcaatct tgttaattat agcattttct tggctttcat taaactagct
    62161 agttcaaaat catttgtttc tttttatgga tttttccttg tcaattcgtt gatttcgcgc
    62221 taaattaaaa gaaagcgagg cgtttgaatg gaaaaaaaag gaaaaatcaa gaaattagat
    62281 aagactcaga tatattttat tctaaaagga atatttgttg gactgctgac aggaatcgtc
    62341 gttagcctgt ttcgattatc tatcgaaaag ctaagcgatt tagttcgaag tatctatgaa
    62401 atgagtcggg aacagccgat ttacttaatt ggcctagcta tttgctgcat ccttgcagca
    62461 tttttcgtgg gttatctggt caaaaatgaa ccggatatca agggaagcgg gattccacaa
    62521 gtagagggac agcttcgcgg ggaattatca atgaactggt tctctgtttt atggaaaaag
    62581 ttcattggcg gtgtactttc agttggtgcg ggcctttttc taggtagaga aggaccttct
    62641 atacagcttg gcgcatccat tggacaagga gctggacaat tgtttcggag tccatcttca
    62701 gaagaaaaga ttttaatttc tagcggtgca agtagcggac ttgcagcagc attcaatgct
    62761 cctatcgcag gattactctt tgttttagaa gaaatccatc actccttttc tccacttgtt
    62821 tggctgacat catttgcttc agcaatcact gctaacttcg tctctcttta ctttttcggc
    62881 ttgagacctg ttttatatct aggtgacctt ccttctttgc cgttgaaata ttatggttcc
    62941 ttagtgatct taggagtact tctaggcatc ttaggtttta tttatcagaa ggtcttgctg
    63001 gctttgccaa aattttacaa ccgattgcct ttaccagctt attttcacgg gttcgttcct
    63061 ttcttgctga ttttgccaat tggttattta tggcctagta ctctaggcgg aggcaatcaa
    63121 atcgttttag cttttggaca tgcttccttg ccaatcatta tgattatcgg tttgtttgtt
    63181 cttcgattcg ttttttccat gatttcttac ggtggtggtc ttccaggggg gattttcctt
    63241 ccgatattga cattgggggc tttaatcggt agcttttatg gaaacattat ggttgatttc
    63301 gggatggatc caatctacat caaggatttt gtcgttattg ccatggctgg ttatttcaca
    63361 gcgattggaa aagcaccgct gacagcaatt attttagtga cagaaatggt tggctctctt
    63421 aatcatctga tgcctttagg attggctgct cttgttgctt atatcgtcaa tgaccttctc
    63481 ggcggtaatc caatttacga atcattattg gaacgtctac ttggaaatac tcagaccgcc
    63541 tcactaaaag gtgcaaggac aacttttgat tttccagtaa cggcagagag ttctttagac
    63601 ggattaatgg tacgagactt caattggcct aaaaatatgc tgctaatctc tatacgcaga
    63661 ggaaatcagg aaatcttgac tcacggtgat acgatcatga atgtaggaga tattttggtt
    63721 atcttgactg atgaagcaaa cttgccaaga atcaagcagg aaatcgaggc taaatcttct
    63781 atcacaaatt tgaagcaatc attttaacga acatggaaaa actattttac aacaactatt
    63841 catctacact acttgattct atatgattct ataaaaaaag aagagactgg gaaaaaccag
    63901 cctcttcttt tttatttcta tgcttctgct gctgcaggag cttttctcaa taaaccataa
    63961 atcacaccag atacgactgc tccgatagca ataaacacca aatacatgac cgggtgactc
    64021 agtaagaata caacgaaaat cccgccatga ggagccagta atttgatccc gaaagctccg
    64081 actaatccgc cagttaaagc agaaccaata ataaaacttg gaatggcacg aatcgggtca
    64141 gctgctgcaa atggaatcga tccttccgtg acaaacgata gtcccatcac gatgttcgtc
    64201 aaaccagcat cttgatctgt tttgctgaat ttattcttaa acaaacgtgt agcaacgaaa
    64261 attgccaatg gaggtaccat tcctccagcc ataacagaag ccatcacgac acttccgcca
    64321 gttgcaacag tcgtagctaa agttgctgta ccaaacacat aagcggcttt gttgattgga
    64381 ccgcctaaat ctgccgccat cattccagct aataatgcac caagtaaagc agcattcgaa
    64441 ccactcatac ttgtcaagaa accgttcaat gcatcattga ttgctttcat agggacattg
    64501 accagcaaca tcaagaaacc agtaatcagt aagccaaaca ctggataaaa gaggatcgtt
    64561 ttaattccat ccaatgattt tggtaagttc ttgaataatt tacgtaaaaa tacgatgaca
    64621 taaccagcta agaatccacc aatcagtgca cctaagaatc ctgcaccgcc tgtattagct
    64681 aatgcaccag cagcaaatcc aacgatcaaa ccagggcggt caccgatact tgaagcaata
    64741 aatcctgcta ataccggcag catgaaacca aatgcagctt gtccgatttg gttgaaccag
    64801 ctagcagctt gattgtaatt ccctagattt gataattgat catgtggcac gccaataaat
    64861 tggtcgatca tgaaagaaag cgcaatagcg attcctccac caataacgaa aggtaacatg
    64921 tgtgatacgc cgttcatcaa atctttgtaa attctttgtc cgatagtacc atccgcattt
    64981 ccttcttctg aactgctttc ttttccatca ccatggaata ctggtgcagt accactaagg
    65041 gctagattga tcagctcttc tggtttacgg ataccatcac tgacaggacg attgatcagt
    65101 tcttttccgt caaagcgatt catctctact tttttgtctg ctgcaacgat tacaccttct
    65161 gcacgggcga tgtcttcagc agtcaatcgg tttttgattc cttctgaacc gttcgtttcg
    65221 actttgatct ccacacccat ttctttcgct tttttcttca gagcatcttc tgccatgtaa
    65281 gtgtgagcga tccctgttgg acaagctgta acggcaacaa cgaattttct ttctggatga
    65341 gcagaagctt ctgcagcttg tgtttcttgt tctgcttttt cttctgcttc tttttgctct
    65401 tcagctgttt ggaacaagtc ttggacttct tctggtgtat ttgcttcttt taattttcct
    65461 acgaaatctg catcaatcag caatcgagat agtgctgcta atgcttgcaa atgtgtgtcg
    65521 tttgcgcctt ctggagctgc gatcatgaaa aacagataag ttggctgtcc gtctaaggct
    65581 tcataatcta ctcctttttg gctttttgcg aataaaactg ttgcttcttt aactgcactg
    65641 tttttcgcgt gaggcatggc gattccatca ccaagacctg tagaagtttg tgcttcgcgg
    65701 gctagaattc cttctttata tgtttcgata tcggaaatcc ggccggcatc aaacattttt
    65761 tggaccattt catcgattgc gcctttttta tcagtcgctt gcaaatccat gatcatcgct
    65821 tctttgacca ttaaatcctt gatattcatg attatcctcc ttgaaactaa tcaatttttg
    65881 tgatttcaac ttctggcagc aactcatcaa tcaattcgcg agctgccaag tcatcagaaa
    65941 aagctgttgc gcttccgcaa gcaactcccc atttaaaggc ttcgattgga tctttcgtac
    66001 ggctgtaagc cccaataaag ccggcaatca ttgaatcccc tgcacctact gaatttttca
    66061 atgggcgaac taatacattc gttttataag ttccttgttc cgtgaagagc aaagcaccgt
    66121 ctccagccat tgagatcagc acattctgtg ccccttcagc tagcaatttt ttaccatacg
    66181 gaatgatttc ttctacagat tggaaggttg tattgtaaag ctcagctaat tcgtgattgt
    66241 ttggtttgat caacaatggt ttcttaacaa gtgcattttt taggtcatca cctgttgtat
    66301 caatcacgaa atctgcacct ttttctcgaa tgatatcaat cagttcttga taaaatcctt
    66361 ttcgcaggct agccggcgcg cttcctgaca atacgacgat atctccttgt cctactgttc
    66421 ctaatgcttg cttcaactca tcgatttctt catctgaaat agatgggccc aaaccgttaa
    66481 tttcagtttc agtatctgat tttagtttga tattgattct tgtatcttct tttacggttg
    66541 tgaacaaaga ctggatttgt tctttttcaa gccacttcga aataaattcg ccagtgaacc
    66601 ctcctaaaaa ccctagtgct gtagattgag aatcgatccg tttcaaaata cgggaaacat
    66661 tgatcccttt tcctccaggt aacttgaagt cttccttcat tcggttcaaa tcgcctagtt
    66721 ttaacccatc tacgcgtacg atataatcaa ttgatggatt aagtgttact gtgtaaatca
    66781 ttgtattgcc tccttaattg ttgtcttttc acggaattca ttaacgaatt ctaatggaca
    66841 acgctcggtt atgatcgtgg ctgcttccaa aggtgcaaca gaggtgaagg acaactggtt
    66901 gaatttggta tgatcaatca atacaaatgt ctcttcggaa tttttcatgg ctaaacgttt
    66961 aacagctgcc tcttctgggt ccggagtcgt gaatccttgt tcaagatggg caccgttcat
    67021 tcccataaat gctttattaa aatgaaaatt ttttagttgt tctatcgcat tagatcctaa
    67081 aacagcatta gttgccagct ttatcgttcc acctaaaata atggtagcaa gctccaaatc
    67141 aactaaattt gccgcatgtt gaacggaatt tgtcactact gtgatctgtt ttccttgcag
    67201 aaaaggaatc atttctaacg tagtagagcc agcgtctaaa taaatgacat cttcgtcatt
    67261 aatggtttca gctgccgatt tggcgatttg ctgtttttcg tgaacgtttt tgagtgattt
    67321 ttcactcata ttctgctcaa aattcaataa ttgctctttc ttagctccgc catgaactct
    67381 ttctagaaac ccactattct ccagctcttg taaatcacgt cttatcgttg attcagaagc
    67441 attaaaaagg tttacaagat cttgtgattt cacgattttc tgctgttcaa gcaagtctag
    67501 gattttttgt ctacgttcct ctgtaagcat tttcacgatc acctcaatac aaataatagc
    67561 atattttttt tctactcgca atataaatca gtcaaaaacg ttcataaaaa ttataaatca
    67621 gtcaaaaaaa aataaaaaac cagtcaaaaa gactggtttt ttattttttt gtctatcatc
    67681 aagccgtttt atcgtttttt attttcgctt cttgtttttt tcatataaac agaagcaggt
    67741 tttctttcct tatgaagaag ataataataa agtcccatcc cgccaaacat gaccaaaaaa
    67801 ccgatgactg atcgaatagg aaaattagga acaaaaaaga gacagacgga taataatgat
    67861 gccggaacca ttattcgata atagagttgt ttatccattt tcttcacctc atattttttg
    67921 taaagtaatc aattgttcta attctcggta atctaaaaat tgcacacgca gatgctgctc
    67981 acctttcttg acatcgaatt ggatacttcg ccgctttttc tttattcgtt tgaaggtaac
    68041 atcagagagt ctatgtttga tttcctttaa cggtaattct gacagggatt ctgaaggaac
    68101 atagattctt aaggataaaa gatactcttg atatttttca tctgttacct ctacttctag
    68161 atattcccta atataataac aataagcttc gattccatta tccaaagcaa cagtcggatc
    68221 gataacttta tagcacatca tgcgcgagcg attctcgtca acccaaggaa aatacgcctg
    68281 agcatttagt gttccgcgaa cacgcctttc tttataatct ccttgttgaa tatcataact
    68341 ttttgttaca aaaaatgaag cattcttttt aggaacaggt tgcatttgtg cattctgata
    68401 ctggacagat tggatcgtat ggtcagtgta aatgtattca acgaatggcg gtttcaataa
    68461 accgacacca ttgtcccaag caagatgatc aaacccaagc tggtccctaa aataggattg
    68521 aaacatttga ttgtttggct tttgccagtt aaaggggtct cgataaagct ggatacatat
    68581 gacttcgttt tctaattcct tcactttttc tacttgttgg atgtcgagaa aaatcggttt
    68641 agggatgatt tgatgataat agcgactata atacatttcc caacacgaag tgaaacataa
    68701 accttggtaa aaaagatcgt atcctggaaa ttggttgcaa tcgactacta cttccccgtt
    68761 ttgttgtttc attttaggta aatgttcgat ttcatccttt ttctcaaaca atagccgttg
    68821 agagatctgt tttgtattgt gatagataaa ctcgctataa gctcgcatat aggcgaacac
    68881 cccatgagca atcatttttt tctgaatgaa gtctcgaatc aaaaattgct tttctttgaa
    68941 aacagagata gacaatacat tttttatcaa taattgatta tcttccaagc gaaaactaca
    69001 ttcgttctcg tcatttccta caactaacca actttcgtct cttaccgatg ctagcattat
    69061 tttttcttga agctttcgaa gatcatacgg atccgcttgt tgtgtgaaag agcggataaa
    69121 tgttggctgg atcaacagac gtgtcaaaat agcccaatcg cttatttcct gttttttttc
    69181 ttcttcattt aagtctgtga gctggatagt catcaataat ttatcttcgc caatcatttt
    69241 ctcacctctt taaaatcttt ttataattta attataacca acgataaaga aaaactaaaa
    69301 ctttatgtct tagaaaccaa tttggacgat tacatgatag actaagtaaa aagaaacaaa
    69361 gaggtgttta caatggggtt gaaatttgaa cgtacagata atttagataa acttttcgaa
    69421 tcatttgctg tcgatccgga aaaaacagtt gcaccaaaag aagagatcga tcgatttcta
    69481 aaaccacagc cacaaaatga aaagaaaaaa gacaagaaac aaaagtaagt taagctcaaa
    69541 aaaagactta ccagccaaac ttttatgaaa gagtttgact ggtaagtctt ttttattctt
    69601 tttatgattc aattgcttgt tcgattgctg cccacgcttc ttcttttccc tgttttgtaa
    69661 cggaagaaaa gagaataaac gtgtcattgc gatcaaaatc caacgctttt ttgatttcgc
    69721 tctcatgctt gttccattta ccacgaggga tcttatctgc cttagtggca acaacaatta
    69781 cagggatatc ataatacttc aaaaattgat acatctggat atcttcttga gaaggcttat
    69841 gacgcaaatc tactaaagaa acgaccgcac gtagctggtc tctagtcgtt aaatatgtct
    69901 cgatcatctt tccccatttt gctcgctcag ttttggatac ttttgcatac ccatatcccg
    69961 gaacatcgac aaaatgtaat gcgttctcga tcaaataaaa attcaatgtc tgggtctttc
    70021 ctggcttacc ggaagtacgg gctaaattct ttcgatcaat caatgtgttg ataaaagaag
    70081 atttccctac atttgatcgt cctgccagag ctatttctgg caattgtgta tctggatatt
    70141 gttttggtgc cactgcactt atgataattt cagcttgatg gactttcatg gtctctctcc
    70201 ttaacaaggt gatgaataaa gcaagcggct gtcgcaaaag ccaaaatgac ttttacggca
    70261 gcctcttttc atcaacccgc ttttttctct gaatctttat agattactgt tggctcacca
    70321 gacaattcag ctgcttcttt tgtgataatc actttttcga tgttttcatt tgaagggata
    70381 tcaaacatca catccatcat gatttcttca atgatggaac gaagtcctcg agcaccggta
    70441 ttgcgttcga ttgctttttt cgcaatggct ctcaacgctt caggttcgaa ttcaagttcg
    70501 gtattatcta gagaaagaag tttttgatat tgtttgacta gcgcattttt cggctcagtc
    70561 aagatccgta ccagatcatc tgttgtcaat ttctctaaag ctgccataac aggtaatcgt
    70621 ccgataaact cagggatcaa tccgaatttt agtaaatctt caggaatgat atgctgcatg
    70681 acactttcgt cttcagaaag tttttggttt tgtgtaccaa atccaatcgt cttttcgccc
    70741 atacgattct tcacaatcgt ttcgatgcca tcaaatgcac cacctacgat gaacaatatg
    70801 ttcgtcgtat caatctgaat aaattcttga tgtggatgtt tacgtcctcc ctgaggtggc
    70861 acgcttgcaa cagttccttc tagaattttc agcaaggctt gttggacacc ttcgccggaa
    70921 acgtcacgag taatagatac attttcgctt ttacgcgcaa ttttatcgat ctcatcaata
    70981 tagatgatcc ctttttctgc acgttccaca ttgtaatcag cagattgaag caatttaagc
    71041 aaaatgtttt ctacatcttc ccccacataa ccagcttctg tcaaacttgt cgcatctgcg
    71101 attgcaaaag gtacattcaa tgtttttgct aatgtttgtg ctaaaaatgt tttaccagac
    71161 cctgtaggtc cgatcaagca gatattgctt ttttgcagtt caacatcatc tgtgttttct
    71221 tgtgcttgct gattgacacg tttatagtga ttgtatacgg caacagctaa cgagcgtttt
    71281 gcgcgatctt gtccaatcac gtagttattc aatacttcaa gaatttctag tggtttaggg
    71341 acttcagtga attcgcgtac agcttcttca taaaattctt catcaatgat ttctttacat
    71401 aggtcgatac attcattaca gatgtagacc cctggaccag ctacgatctt tttcacttct
    71461 tcttgggttt tcccgcaaaa agaacagcgt acggtctcat tgctacttgg attgtcatac
    71521 atggtcttca ccccttaact tgcaaaaagt atgcaagaaa ctatcaaaga ctttacagtc
    71581 ttttactatc ataacatagt ttagtaaaaa gaaaaagtgt ttaacaaagg atcaactgac
    71641 ctataccttt gcatttttta ctcttaacaa aaaataagga actgtgacaa aatcgtgtca
    71701 cagtccctta tttcgaataa acaattttct agaagtagct tcttcagaaa tgatttatta
    71761 cttgatttct attctttcgc ttctgtcgct gtacctgtga tcaattcgat tgcttttttc
    71821 attgtcacat catgttccag catatcttct gtcaacacgc gtttgatttg ttcgattggc
    71881 atgttgtatg tttctgataa ttcttggatt tctgcattga tttcttcttc tgttgcttga
    71941 agattttcag cagctgcaat tgcttcgatc acaagatttg ttcttgtgcg catttctgct
    72001 tcaccttcga attgtttatg caagtcttct tctgtagaac cagttaattg atagtacatt
    72061 tcaggagaga taccttgacg ttgcatgttg ttcaagaatt cgtccattga acgatgtact
    72121 tcatcatgaa ccatcacgtg tggcaattca acgatttctg cgttttcaac agccatacgg
    72181 atcgctgctt catcttttgc atcgtccgct gctttttctt ttgtttctac taattctttg
    72241 cgatattttt ctttcaattc gtctaatgtt gatacttcgt catcaacatc ttttgcgaat
    72301 tcgtcatcta attcaggcac ttcttttgct tttacttcat ggattgtcac tttgaagact
    72361 gcttcttttc cagcaagatc ttctgcttga taatcttctg ggaaagttac atttacatct
    72421 agactttcac ctgatttatg accaactaat tgatcttcaa aacctggaat gaatgagcct
    72481 gaacctaatt caagtgagta attttcgcct tttccgccat caaacgcttc gccatctaca
    72541 aagccttcga agtcgattac tactgtatcg ccattttcag ctgcagcatc ttctttgata
    72601 actaattcag cttgtgcttc ttgttcacgt ttgatacgtg catccacgtc agcatctgtt
    72661 acttcacggt cttgtttttc aacaactaga tctttatatt gtcctaattt tacttctggt
    72721 ttcactgtta cttcagcagt gattacccag tcttgacctt tttccatgct ttcaacatcg
    72781 atttttggtt gagaaacagg atcgatgcca gcttctttga cagcagcttc ataagcttct
    72841 ggtaatacag cattcaatgc atcttcatat aaagcttctt caccgtacat acggttgaat
    72901 acttgacgag aaactttccc tttacggaat ccaggtacgt tcaagctttt tttcacttta
    72961 ttaaacgcag ttgttaatcc tttttggatt tcgtcttgcg caatcgtaaa ttttaaaaca
    73021 ccatcattcg tgcctttttt ttcaaatgtt gcagtcattt aattccctcc gagtttagaa
    73081 attcatttta tacatcaaaa acgtttcatg cataccttta aatagtacac taaccctttc
    73141 aaattgtaaa catattgaat ggatagaatc cttgtttttc ataacatttt tatctttttc
    73201 actgataaaa tctaatggaa gaagatcatt tgctgcaaaa ttttttctct tttttttaaa
    73261 aaatcttgtt tttcatcttc tgtaccttca tacatcgttt ttcctgtaca cgcctctaca
    73321 gtcagttgaa accagcgctt cgggtcttcg atgtatgtat catagacagg ataaacaatt
    73381 gcagattgca acgtgaattc ttcatttaac acggaactaa gcagcgcatc ttcattaccg
    73441 taacgatctg aaaatacaca taattgctgg taacttgcct gctgctgcgg ctttggcaga
    73501 tcttttggta tgactttgac taacttttct tcaatcgtca aatagaaaac aggatggcat
    73561 tcgttcaatt gcctaagtgt ctccagtaat tttgcccttg ctaaaatatg gacctctggc
    73621 gatacaataa gtttactcgc tgtttggata aactcatgat aaggaagctg ttcgatctca
    73681 tcaatagacg caagctgacg tgcaggttcc aacgaaggaa gttcctccga tttattgtgg
    73741 agtgccttga tcttctgcct ctccatttgt ccggataatt cttctgcaaa ttggattttt
    73801 tcaatgaccc gctgctcgaa agcttcataa aaatctgtac ttgctatcaa ttttctagca
    73861 tataaaaaat cttgtgtact gattaacaag tcaaaataaa attcagcaaa ctcctcattt
    73921 tgaagatact gtttttcatg caagagtgct tgcttcaatg cttctttttt ctcgtccaac
    73981 tcgtaaagac aaaaaacaag caggctatta gcctcaaagc tttctttcaa ctcgtaagct
    74041 cgttgaaata attcttttgc ctgctgatat tgctggtttg ctagtgcttg ttgtgctact
    74101 cgcattaaat tctcatattt ttctggaaaa tcaatcggct tcacgcagtg tcctcctctt
    74161 gctcatgttc agctcatgtt caatagtagc aaaacaaaaa aagcatgaca attaaaaata
    74221 actgaagcac cctctaaaag ctagatttta gatctgactt ttagaaggtg ctttcaactg
    74281 ttactgcttt ttattgctca tcagctgttg acttcctggt tgaatgcttt gatcagattg
    74341 atcgtaacaa cacttgtatt agtgaaaaaa tcgcctagat gttgcttttt ctgattaaag
    74401 attgctggaa gataccctaa catcagcaaa ggaaatttta gaatgaaccg gcaagcacct
    74461 tctctaacta aaacggttga ccagctaagt tcttcttcat caaagctgat gactcgaata
    74521 ccaaagatca ttttcccaat cgtttgacca tgattgaatt tcgtcaataa aataaaatac
    74581 gctagataaa taagtaaggc tgtgaatccg tagatactca gataagaatc tgaaaaatta
    74641 agccaaccta gatttttggc aataccaagt gttaaacgcg taatagctcc gatacacaaa
    74701 agatcgacca agaaagcaaa cattcggata aaaaaacctg catagaaata cttaggaaaa
    74761 tcatttgctt ttggcggttc atttcttgtg tccgtatact ttttccattt acgttgtttc
    74821 tttttgattt cttcgggcgt caaaggctct ctttcaaaag agctcagtac cttttgggaa
    74881 aagttggagt caggtcctac agcaggctgc tgttgcttat ctttttcttg ttccttattt
    74941 tcatttgaaa gctctgacat gcttactcac ctccgtaata atacattgct tttggtgatt
    75001 ccgctgtccc tagattctca agaacggata agatctgact agtctcagaa ggcttcaatc
    75061 cctggaattc tgctagttta cttcctaacc atgtactagc aaacccagat ggcgtggaag
    75121 aatattctac tagttccgca tcttcaagct gttgttcctt tcgcatagct tttaaagcat
    75181 cttcaggaaa tcccaattca tcgactaaac ctacttcttt tgcttggaca ccatcataaa
    75241 tccttccatc agcaattttt ttgacttctt cttcagattt attccgtccc tcgctgacga
    75301 ttgctacaaa acgactatat gcgctgtcga tatatgcttg aagcaccgct tcttcttctt
    75361 ttgtctctgg tcgcgttcct gaacccatat ctttcaacgc tccgctttta actgttgtat
    75421 cttccacacc cagtttttct agaagtcctg aataattcaa tcctgacatg atcacaccaa
    75481 ttgagccggt cactgtttct tctgttgcaa agattttatc tgcttgagca gaaatatagt
    75541 agccaccgct agctgccatg tttttcatgc tgacatacat tgggatatca tgttctttac
    75601 gaatcgtatc caatttctta gcgatttccg cactttcgta gacaccgcca ccaggagaat
    75661 ttacttctaa aaagatacct ttgactgttg gatcttcttc aatcgccttc agttgttcca
    75721 taaaattttt atgattgtaa ccttcagaag caaataaacc tgaagaccct cccgcagcaa
    75781 tcgttccatc tactgataat tttacgattt tttgcgtact gtctccctct tccaacacat
    75841 ttgaagctag ttcttcatcc ccatataacc aactgttcaa tccctcaatt tgatcgtttt
    75901 cctgaggctt agtcaaacta gctgaaacta acgaaaccac aaataatcct gcagcaatca
    75961 gaactgctgc ccaacgtctc ttattcattc tttctccttt ctaatacaag ttttttatga
    76021 cgatcacagt ttctgactgc tcagaagtgt cgaaccgctc tgctttttct tcccgcgcat
    76081 acccggaaaa cgactctttt gtcaattcat cttctaccgt aaactcacga gcttcagcaa
    76141 cttccaaggc atcaggtgcg atttcttgga tgctatccca gccagtatct aaaaatattt
    76201 tcgtatcatc tgatacacca gctgatgctt cgaatttgga caattgtttt tttaacattc
    76261 ccagtgtcat gatctcaatt tttgaatcca tttttactcc tcctctatcc gattactttc
    76321 tattgtaccg aactcctgaa aaagatgata gataaaaacc cattattttt tgacataaaa
    76381 agagcatttg acaaagcatt tgatatatac caatttattt tttattcttt ttttgttgac
    76441 aagaacattc gcaaaaggtt atgattttaa atgtttccag ataaaaaaca aaagaggagg
    76501 atagttatac cttttaggat agcgccaggt ctgtgtaaac agatgacgag gagagggttt
    76561 atcgaaagat tcggcggatg gccctaggga ctgcactcta cagaaaatcc acaaaaaata
    76621 tctgcaaaga taaaacaaaa ggattttcct gcagctggaa gcattcatac gaattcatta
    76681 tagaaagtag gatgtttttt atgtgcggaa ttgttggaat gattggatta aaaaatgtaa
    76741 caccaggatt gattgacggt ctagaaaaat tagaataccg tggttatgat tcagctggta
    76801 tctttgtctc agatggcaca actgattatc tggtcaaagc acaaggcaga attcaaaatt
    76861 tgaaaaacaa aataaatatt gatactacag gaaatatagg tattggtcat acacgctggg
    76921 caactcacgg gcaaccttct gaagaaaacg cccatccaca tacttcccaa agtggacgct
    76981 tcgtgctcgt tcacaatggc gtaatcgaaa actttgaaga acttaaaatt gcttatttag
    77041 caaatgacca ctttattggt gagaccgata ctgagattat cgctcatcta attgaaactt
    77101 tcgcgaagga tactacaacg caagaggcgt ttttaaaagc tttgcgggtc atcaaaggtt
    77161 cttacgcttt cgctttgatt gatcggacag cacctgatgt tatttacgtc gctaaaaata
    77221 aaagtccact attaatcgga ctcggcgatg gattcaatgt gattgccagc gatgcaatgg
    77281 ctatgcttgc tcatacgaag gaattcgttg agattgaaga tgaagagatg gttaccgtca
    77341 catcagaaaa agttattatt caaaattttg caggtgacat cgtagaacgt tcttcattcg
    77401 aagcacaagt agattcttca gatatcgaga aaggtaccta tcctttttat atgctgaaag
    77461 aaatcgacga acagcctatt atcatgcgtc gtatcgctca aaaatacgta acagaagaca
    77521 atcgtgtctc tcttgatcaa aagctggtcg atacattgtc tgatagcgat cgaatctata
    77581 tcgttgcttg cggaactagt taccatgcgg ggttagcagg taaacagact ctggaaaaat
    77641 tgacacagat ccctgttgag gttcatttag ccagtgaatt tggctataac acacctcttc
    77701 tttcaaaaaa accattcttt attttcttga gtcaaagtgg cgaaacagca gatagccgcc
    77761 aagttctggt aaaaatcaac cgtctcggct acccttcact tacgattaca aatgttgctg
    77821 gttctactct ttcgcgggaa gcatccttta cactgctatt gcatgctgga cccgaaatcg
    77881 cagtagcatc aacgaaagct tatactgcac aaatagctgt actaacttta ttagccaaag
    77941 ctatcggtga taaaaaagaa tttgaaactt ctcttgtatt cgatatcact catgaattga
    78001 gtttagttgc taatgcgatg gaatcggtca tcgcccaaaa agattattta gaagagttgg
    78061 cagaagtata tctcagcgat acaagaaatg ccttttacat tggacgtggc gctgactaca
    78121 acgtttctct tgaagctgct ttgaaattga aagaaatcag ttatatccag gcagaaggtt
    78181 ttgccgctgg cgaattgaaa catggaacga ttgctttgat cgaagaagga acaccagtga
    78241 ttggaatcat cagtgaagaa gtgactggtg cgcatactcg aggaaacctg aaagaagtcg
    78301 aaagccgtgg cgctaaaaca ctagttattg tctcagaggg attagaaaag gaaagtgacc
    78361 agctggtgct accaactgtc cacccttatc taactactct aaccacagtc gtaccgactc
    78421 aattacttgc atattacgca acgctattac ggggattcga cgttgacaaa ccacgtaatt
    78481 tagcgaaatc agtgacagta gaataataaa agaacaagga ccagtgtcag acatctgaca
    78541 ctggtccttg ttcttttatt ttttatgaaa atatctctta atccagtccg atttcttctt
    78601 tgactactgc cgcgatttta tcgacatagt aattaacttt ctcatcagta ggcgcttctg
    78661 ccatcactcg aagtaatggt tctgtaccgg aaggacgaac taggattctt ccttctgatc
    78721 ccatttcagc ttctgcctct tcaatcactt ttttgattgc cggaacatcc atcgcaccgt
    78781 ttttatcact tactcggata ttcactaatt tttgcggata gattgttacc tctgctgcaa
    78841 gctctgataa tttcttacct gtctgtttca tgatattcag taactgaatt cctgaaagca
    78901 tcccatctcc agttgtgtta aaatctaaga aaatcatgtg cccagactgc tcgccgccaa
    78961 agttataatc gtttttgcgc atttcttcta caacgtaacg atcaccaact tgtgtaacga
    79021 catctttcaa accgattcct tccaccgctt tgtggaatcc taagttactc atgacagtcg
    79081 taacgatcgt atcttttttc aatcgatttt tttctgccaa gtatctagca cagataaaca
    79141 tgattttatc accgtcgaca atgtttccaa gttcatccac cgcaataata cgatcgccat
    79201 cgccatcgaa agctagtcca gcatctgctc ctttttcaac gaccatctca gctaaacgtt
    79261 ctggatgggt agagcctacg ccatcattga tatttaaccc gtctggattt gtgcccatgg
    79321 tataaaaatc tgtttcaaga tcggcaaaca aacgattaac acttgttgaa gttgctccgt
    79381 ttgctgcatc gacacaaatg gtcaaaccgg aaaggtcacc gttgatcgtt tgtacaagga
    79441 attgagaata tttcaataag ccttctggaa attcttctac agttcccaat ccttcagctg
    79501 atggacgagg caattcatcc acttcagcat ctaataatgc ttcaatttct agctcttggt
    79561 catctactag tttaaaacca tctgcaccga aaaatttgat tccattatct tgtgctggat
    79621 tatgagaagc agaaatcatg acccctgcgc ttgctttttg caaacgtgtc agataagcaa
    79681 cacctggtgt agagatcaca cctagctgga acacttcgat acccaccgat aaaagtcctg
    79741 aaatcagcgc ttcttctaac atttgacctg agattcttgt atcccgccct actaacacgc
    79801 gtgggcgttt tccttcttct tcatgctggc ttaggacata acctccgcac cgtcccagct
    79861 taaatgcaag ttctggcgta agttccttgt tggcttcacc gcgtacgccg tctgttccaa
    79921 aatatttacc catagttgta aaattactcc tttaacttaa ttttctgagg aatttgttct
    79981 gctcatttgt gttgattcgc ttgtttctga tgtttcttct gtacttgtgg tcgagctgct
    80041 tgtcccagat tccgaactgg aagaaatggt tgttgtcgat ggttccgttc ctgaatcagc
    80101 agatgaaata cttctactcg tacttgtatt ttcttgtgtc cgactgctcg tcgaagcaga
    80161 gctactgctt gacgtttgca acacgggtgt aatctccaca ctaactgatt cgggcttgat
    80221 acttaacccc tcttctactg gtatttcaat tttacgagtt gttcgatgat tgatacctga
    80281 gacattgatc ggtacagcaa tactgtcacc gatcgatgca agcaaatcgc tagagccgct
    80341 tacttctgct tccagttttg aaaaactgaa atcatagctt tctaccccgg cagctggtgt
    80401 tccttgctgt atcccataaa gtcgtaccga cttgcttggc ttgatcgaat cagctctcac
    80461 tgttacttgc actggatcag agacgatact taatacttcc cctgctgcat tcagcgcctg
    80521 tattgggacc tcactattaa tcccatcatc tgtgaccatc gaagcgtcca catcagctac
    80581 tactcgtttg atttctgcca atgttttgtc acctgtagtg atagagactt ttttaggact
    80641 aacggaagta tcatcaagtt catatccaga tggtgtgaac gttgaaggaa tcaacgtttc
    80701 cacatcgaaa tcttttatta cctttttctc aaccgttact gtaatagtcg atggttcgat
    80761 ctttgctgtg acagcactgc tcaaattttg aatttttagc ggaacctcat gtgttccttc
    80821 gcccagctta gtgatatctg ctgtgacgcg gaacatccgt gtatcttcgt ttgcctctgc
    80881 attcaactga actcggtttg cactggaaag tttgacagtc actgcatttt catatccatg
    80941 gatatagtag cgctttgaat cataagaaat cttgattggc accccagtaa ctgtttgatt
    81001 atacgcttca tttccagaaa gcgtatttgg aacggactgg ccatttgcat tgaagtacag
    81061 cgcaatactg aacagcaatg caataaaact gtaaagaatg cttgtccgtt tctcttttga
    81121 gatcatttgc gatcccctcc cttagtcata ccgtctagga aagattgaat cacgtttttc
    81181 ttgttttttg ttttctcttc agcaatcagt tctctacgca aaattgccaa atattcttgc
    81241 tgggataagc cagccatcag ttcattatct aatgtaatgc tgacatcccc agtttcttca
    81301 gagacaacga tggtaatcgc gtcgctgact tcgcttatac cgacagctgc ccggtgtcga
    81361 gtcccgaatt cttttggaat caacatactt tctgatagag gaagataggc gcttgctact
    81421 gcgatctttc cttcttttac aataactgca ccgtcatgca gcggtgtatt aggaataaag
    81481 atattgatca gcagctctcc agtgatatct gcatctaatg caattcctgt ctcaatatac
    81541 tcatctaatc cagtatgccg ttcgatagta attagcgcac cgatttttcg cttggacata
    81601 tattggatgg ctttgtcaaa agacaagatc atcttctcat cttcctgctg ttcgcttttt
    81661 gattgtttga aaagcgaact gcgtccaaga tgttccagtc ctctccggat ttccggctga
    81721 aaaatcacga tagcagctat caccccgtac gtaatgactt ggttcatcag ccaagacaag
    81781 gtgtgcagac caatgatttc cgctacgatt cggataacaa taaaaatagc tacacctttc
    81841 agaagctgaa tcgcttttgt gcctcgcagc agttggatca gtttgtatac aagataccag
    81901 acaaccagaa tatcaagtat gttcaccaaa aaattcagag agaacaagtc accggaaaac
    81961 atttgctgcc aatagtccaa attaaaaagt tccccgattt gaaatgacat aaaaagcctc
    82021 tcttctcttt tgagatttcc tctctagtat atcatatgtt ttaattttag aggctgatct
    82081 gctcgtattt ttcttgagaa aaacgcataa ttttcaggaa aattgcaagt atgcgtttta
    82141 tttttctctg caataaatat ttttaaactt cagagaatta ttcattatat tttttctgtt
    82201 atttttcctt tttttctttt gtagcttgtt ccattttttt acgtcgttct gctctggcag
    82261 ctcttctttc ttctgcttca aggtccgtcg ttttttcttt tctagtctta acgggtacat
    82321 cgatagattc ctctggttca agtcgtttct tgattttttc taattctccc gtcagtgcag
    82381 catagttgta aaaacgtgtt ttagcgtctt ggatagtctt ttcaaataaa gcttttgcaa
    82441 gatttggatt tgcgttctct agcgaggaaa agcgaacttg tttcctcatg aaatcaggca
    82501 tagcagagaa atcaggtttt ttgtagtcca aagtcattgg gttcttgttt ttttcgacca
    82561 ttgcagggtt atatcgatat aatgaccaat agccagaaga aacagcttct tttgcttctt
    82621 taagcgattc tttcattcca ccaagcaaac catgtgtgat acaaggcgta taggcaatga
    82681 ttaaagaagg tccaggatac ttttctgctt cttcaaaagc ctttatcgtc tgcatttgat
    82741 tggcacctga agcaatttgc gccacataaa catttccata tgtcatcgcc atcattccta
    82801 gatctttctt tgaagcatac tttccactag ccgaaaattt agcgattgcc gaagctggtg
    82861 ttgcttttga ggtttgaccg ccagtattgg aatagacttc gttatccaaa acaagaatat
    82921 tcacgtctgc tccgctggca agtacatgat cgatcccgcc aaaaccaatg tcgtaagccc
    82981 atccgtctcc accaatcatc cattggctgt tttttacgaa tagatccttt ctcttgtata
    83041 tatcctccag ttcagggaca attgatcgtt cgctttcgat cgcagcttgc agttttgctg
    83101 ctctttgctg cgttccttcg ctctcaaaca tgtgtaccaa ccagtcttcc atcaattctc
    83161 gtagttcttc tgatactttt ggcaatactt ctttgattga acttgccaaa gcctcgcgac
    83221 gagtctgtgc cgctagaagc ataccataac caaattcagc attatcttcc aataaagaat
    83281 ttgaccatgc aggcccttga ccacattcat tggtcgtata aggagaagca ggagaagcac
    83341 cgccccaaat agaagaacag cctgtcgcat tcgcgatcat cattcgatca ccgaacattt
    83401 gtgtcaataa ttttacataa ggtgtttcgc cacagcctga gcaagcgccc gagaattcaa
    83461 gaagcggttg attgaattgt gtagacaaga cagtattctt tcctttgact ggatttgctt
    83521 tttgtcttaa tgtcattgca aaagcccaat tcacagcttg ttctttctgt tcttcataag
    83581 gcttcatcac taaagctttt tcttttgcgg gacaagcctc tacacacaga ccacaacctg
    83641 tacagtcttc cactgaaact tggatacggt attttacgcc gtctgcgcct cgcatttctc
    83701 tcacgataaa tccttctggc gcttcttcca tttcctcttc atctgctaaa aatggacgga
    83761 tggcagcatg aggacaaaca aaggcacatt cattacacat cgtacagcga tcactaatcc
    83821 actcaggtac ttctaaagcg acgccccgct tttccataga agccgttcct aaaggcattt
    83881 cgccagttgt cattccattt gctacaagtg taccaacaga gagttgattt ccttcctgtg
    83941 cattcactgg ttcaagtatt tcttgtatat aaccagtgtt ccttttattg attttttcag
    84001 gcatctccaa cgttctccaa tggtctggga tcgcgacttg atgaagcagt tcggtcgtcc
    84061 gatcgattgc ttgtatgttt ttttctacga tagccattga tttatgtcca tagcttttca
    84121 gggcttcttc ttttagcagc ggaagcacct catcaaaagg aatgatgtca gctaatttaa
    84181 aaaaagccgt ttccattgcc gtattgatcc ggcgtcccaa accgacttct tgggccaatc
    84241 tcatggcatt gatcgtatag aatctaatgt tattttcagc caagtaacgt tttaatttta
    84301 agggcagatg cgtttctaat tgttcgtcgc tccaaagagt attcaataaa aaaatgccac
    84361 ctggcttcaa tcctttgacc aagtcatagc tatgcaggta agctggtgtg tgacaggcaa
    84421 caaggtccgc atgctcgaca agatacgctg aacgtatcgg cgtatctcca aaacgaagat
    84481 gagagacagt cagaccgcca gattttttcg aatcataata aaaatagcct tgcgcatatt
    84541 tatcggtgtg atctccaata atcttgatag ctgatttatt tgctccaact gttccgtcag
    84601 atccaaatcc ccaaaacttg gcctggaatg tttgcggttc tgtcaaatcc agactttcct
    84661 ttggttccaa agattgataa gtcacatcat ccacgatccc tatggtaaac cgttttttgc
    84721 gtatgcttgg gtcttttttc aattcatcaa aaactgctga gatttggtcg ggagtcacat
    84781 ctttagaccc caatccgtag cgaccaccaa taacagccgg gcgaagttca gaatcatata
    84841 acgcactttg aacgtctaat aagagaggtt ctccacctgc ccctggttct ttacttcgat
    84901 caagaacagc aattgttttt actgtatgag gcaacttttg cacgaatgtt tccactggaa
    84961 aaggacgata aagatggata ttcaaaaaac ctgttttccg tccgtttgca ttcaagtaat
    85021 caatcgtttg ttcaatcgtc tgtgcaactg atcccataga aacaatgact tcttccgcat
    85081 cgtctgctcc gtaataagtc accaagtcat aagaagttcc tcgtaatcgg ttgatctctt
    85141 tcatatattt ttgaacgatt tcaggtaatt tatcataata ggggttgatc gtttcccgtt
    85201 gttgaaaatg gatgtctgga ttctggttcg taccactaac agtcggatga ttgggattca
    85261 tccctcgttt acgaaatgaa gctaatttct cctgattgat cagaggagct aattcttcat
    85321 attccaaaac ttcgattttt tgaatctcgt gactcgtacg gaatccatca aagaaattca
    85381 aaaaaggtac ttctccttca atagaagcaa gatgggcaac aggtgctaaa tccatcactt
    85441 cttgtacact gctttcagct aacatagcaa atcctgtttg tcttgcagcc atcacgtctc
    85501 cgtgatctcc gaaaatattc aacgcatttg tcgtcactgc ccgactagca acatggaaaa
    85561 cacttggcag tagttcccca gcaatcttgt acatattcgg tatcatcaaa agcagccctt
    85621 gagaagcggt atatgtagtt gtcagtgcac cagttttcaa tgatccatgg accacaccag
    85681 ctgctcctgc ttcggattgc atttctacta cttttactgg ttgaccgaat acattttctt
    85741 ttcctgctgc tgaccattga tctactaatt cagccatggt cgaactgggt gttattggat
    85801 atattgcagc aatctcggta aatgcataag aaatatatgc ggctgccgta ttgccatcca
    85861 ttgttttcat ttttcgcatg attcatcatc cttgttcata ttctctctta ttatctgttc
    85921 cattataacg taaaacattc cattttttct tttatttata aaaatatttc acatatgtct
    85981 atttgaattg gtcatctaat ttttagacaa acagtctaaa tcgttgcctt tttttgtcag
    86041 acatgtataa actataagcg aaagaggtgt tttaacatgg atatatcagt gattgatgca
    86101 acgaaaacaa atacacaaaa aggaatcctt tatggttcat cgaatgctcc taaaaaaatg
    86161 gtcgaattca tcaacctatc ttgtccgtat tgccgacaat ggtttgaaga atcttatgag
    86221 ctattagaag aagcagtcca aagcgggcaa ttgcagcgag tgatcaaatt gttcgataaa
    86281 gaaaaagaaa gcttgcagcg aggaaacgtg atgcaccggt atctcacaat cagcgatggt
    86341 caaaaagcga tcaaagagat caaacaaata ttcgatactc aagacgagtg gaaacatctt
    86401 tctctccaag aagtagcgga ttttgcagta gataaactaa agctgacaga acaaaaagac
    86461 gaacagctga gccaagctgt catcaacgaa gctgagcaag cacatattcg atttgttccg
    86521 actgtcatct tgggtaaaga aatctttgat gaatctattt caatagagga attaaaagaa
    86581 ttgatccaaa caaaataaaa aaataaggag cgtaggacaa aagtaaagaa cacttttgtc
    86641 tcacgctcta aacacctata aacggcggaa gcagaagcaa ctccttctta tttcttggag
    86701 ttaaacgctt ctgtcccagc ctccttattc cgaataaatg gtgtccaaaa gcttgcatct
    86761 gccagattat ctcatttctt atgacaagct gagcaaatta tctccaccaa ataatttatt
    86821 catatttttg acttgcgcat actaactaat tcttaaattt tcatacaaaa tttctcaaat
    86881 tcccttaacg agtaatattt atgtacggtg attcacacta gtatcccaat cactttttaa
    86941 gttatcccga tttcaattct acttcaaaaa tagttgcttt tatgtatgct gttagttgat
    87001 tagatggtcg tttgttgagc taggtcgttt attttaaaca agttgtttct ttagcttata
    87061 cctatctcgt ctaccaaacg caagatggca aacacaaatg catacgcatc tgtatttgcc
    87121 actttgattc gtataaagct aacaactcct aactcctctc atttaatagt tgctctcttt
    87181 tttattcaga tacatttttg ctgatcacag cctagtattt caatggtttt gaacactaaa
    87241 ccaaattgtt ccattacaac attcactggc tgatgaaatg gattaataga tttcgattat
    87301 tcaaagcgat tttctattgg tggattgtgt tcccaactaa caagacggat tccaaagtca
    87361 ttatataact cttcaagagt taaccctttc ctgttcccaa tagatgtatc tctcccacgc
    87421 tcgatataaa atatggagtt tcgaatgcca agacctggca aatctttcct tgttacagtt
    87481 acactgctac taacttcccc atataaaatc cagctcctcc aaaatgataa tatatttttc
    87541 gaccatcatc taactcatgt aacaataatt gttgtgccct cttgtcactc gccaaatgca
    87601 aacgccacgt gcgataccat gccatgctac ctgcatctgc agaaaatcca tcaaataatg
    87661 caacaattgt atcttctatc acaatccttt ccccatctac ttcatattct aatctaaaat
    87721 caaactcccc tcgggtaatc gcaggtcttg gtggcttagg acccaatatt attctactta
    87781 aaaccgcatt cgttcccaga tgaaacacag ctataacaac aataatgatt aatattttgt
    87841 ttctatttga taaatttctc attattcgca aatcacctct acattctcac atactagttc
    87901 gtaatactcc tttaatatca aaatatctac tgatcgtaat aatattcgct atgaattcct
    87961 actactcgta taagaaaggc caaatcatca gcttttactt gctgatgatt tggcttgttt
    88021 ctatttatcg taattgtttt ctaatcacca aaattgttaa caatcggttc agatatttcc
    88081 caattcacta gccttatatt atattgattt aacaattctt cttcatttat tgtattttga
    88141 tcaagcggac caaattgttc taataattct ttgtcatgta aaatatccct tctagtaatg
    88201 cctctttccc tttcaatgaa agaagcacta ggaaaagtat gattataacc attcaaccta
    88261 tctcccatgt aataattagc aggtcctgga aaataaaata tttgcttatt atcactaact
    88321 tctaacagca acactaaatc ttctccgctg gctaaacgac ttgtccatct acgatatctc
    88381 cctatgcctt cgttcattcc aaatccatca aattcaacta ttaacgtgtc ttctatcaca
    88441 tgtaattctt cattcaattc atactcaagt ctaaaaggaa attctccata tgtaatctct
    88501 ggcaatggtg gtgctggagt tctccaaata aatacaataa ctgtgaatag aaaaaatcca
    88561 acgatcgata taaatgttat gagacctgct cgaagtaaaa cccgatataa taaaaatgct
    88621 gtatttcgct ttgctatatc tagaaaaata aaaagtactg caatcataat gatagtaaaa
    88681 gttgtaaaaa aacgcatttt tgccctccct ttataaacat cgattctcaa attctaaacc
    88741 aaatcttggt agttcaagtt caggtataac gtattccaac catacatctc aattactatt
    88801 tatgaactta tgattatcac tgaataaatc actccatccc cttgttaaaa cttctacatt
    88861 aattacacct ggttgttttc gactagctaa tccaatgaac ttatcaacca tccttatcca
    88921 ccaaccacga gaatcttgac cagaagtaag aagagcagtt ctcaaggatg atcgagcagt
    88981 tccttccagt tttttaatta atcaggccag ttatagataa atacttgatc ttatctatat
    89041 caggacttct atattaagca aaaatttatg tacaaaacta aaatcttcat tcagttgtct
    89101 ccccgctttt tatacgattg tattaaaaac aatagaagcc tttccaaatt atgttactcc
    89161 agtaaagatg gaattatagt ttgacggaag aaaagcaagt ataactttta aaaaaaactg
    89221 atacattcgc atctcctgct tcatgtatca gttttttctt tggattaaac tgcttcttca
    89281 cgcagttttt ccgcaaattc atttattttg cgaattcggt gattaattcc cgattttgat
    89341 atcgcacctg agggaatcat ttcccctaat tctttcaagc tgacctctgg atgttgaagc
    89401 cgaagttctg cgatttcctg aagtttttca ggaagggcat gtagaccgac ctttgcttcg
    89461 atcaattcga tgttttcgat ttgtttagaa gccgcatcaa tcgtcttgtt cagatttgct
    89521 gtctcacaat tgactagacg attgaccgaa tttctcatat cccgtacgat tcgaacatct
    89581 tcaaatcgga gcattgagtt ggttgcacca atcaatgtta agaaatcagc aattttttct
    89641 gctcctttta aataagaaat atagccattg cgccgttcaa gcgttcgagc atgtagaccg
    89701 taataattta acatatcgca aatgtcttgg ttatgctgtt cataaataga aaatatttcc
    89761 aaatgataac ggctcgtttc cggattattg actgaacctg aggccataaa agcaccacgt
    89821 aagtaagaac gcattttttg cgaatttccc ataatctcgt ccgaaacgtg tccattgaac
    89881 atcataccat ccatgatatc aagatccatc aacacatctt ttgtgccttg cttcaaccgg
    89941 acgatataga cattgttctt ttttaatttc atttttttac gaacgagtag ctcacttcgt
    90001 acctgataat gatctttcaa aagagaatag atacgtctgg caattgcagc attttctgtt
    90061 tggacgttca aaataaactg ctggttcaca atacctaaag agccgttcat tcgcagcaaa
    90121 gcagctaatt ccgctttcgc atgttctcga tgaacttcca aaccagttag ttctttcttc
    90181 acatctgcag caaatgacat cctattctcc tcctttctcc aacaataaat tgcttaatat
    90241 tttgaaccga aaactaaacg gaacaattct tctactactt tgtctccatc gtggaagaca
    90301 ccaccatctt tcaattccaa aaagtcggtg gaaatcactc gacatccttc atttctcaat
    90361 ccgttgaagt cgtgtgcgac ttgtactaga tactcatcat agatctctgg gtccatataa
    90421 tcttcaggca ctttttccgt attgaccaat actgtatcga taaaaggctg attcaaatgc
    90481 ttgtgtaaaa cacgtatatg atcggcatct gtgaaatgtt ccgtttcccc tttttgggtc
    90541 atgatattac agatatagac ggtttcagca gttgtcttga gcattgcttc gccgatttct
    90601 ggtatcacta aatttggaag gatactggtg aataaacttc cgggtcccaa aacgaccatg
    90661 tcggcgtcca taattgctga aacgactttt cgagccgctt tggcttcact tttatcattt
    90721 gtgttgcaga catacacacg gtcaatggtt ttacggtcga tcgcgatttt agattctcct
    90781 actgcagtcg ttccgtcttt aaagacagca tgcaaggtca aaggagtttc tgaagaagga
    90841 taaacatgtc catctacatg catcattttt gctaataatt gaatcgcctc ataagtgctt
    90901 ccccgcatct cggaaactgc ggcaatgatc agattaccga tcgtatgatt ggctaaaaac
    90961 tgatcttcct ttttgaaacg gtattggaaa atatcttcat agaattgtgg catatccgat
    91021 aacgccacta agacatttct caagtcacca ggaggagcca tagcgatcga ttctctgatt
    91081 gcaccgctgc ttcccccatc atctgcaaca gtcactactg ctgtgatatc tgctccttga
    91141 tttcgcaaac ttttcaggat aactggcaga ccagtgccac cgccgaccac tacgatcttt
    91201 ggttttctta tccggtaggt tttcattttc acgaccggtt caccgtctct ttccttttat
    91261 ccttgtcccg atgtgtcaca ttgactttat agtttttccc taaagcattt gccaaacgtt
    91321 cagtcaatgc cacagaacgg tgttgaccgc ctgtacagcc gattgcaact gtcaagctgc
    91381 tttttccttc tttaatgtat ccaggcaata cagtttccag aagatccaaa aatttatggt
    91441 agaatgattg cgtttcatca aaggacatca cataatcata gactggctga tcttttccgg
    91501 tcaatggtct tagttccggg atataatgag ggttgggcaa aaaacgcaca tccatcacaa
    91561 tatccgaatc gatcggcaac ccgtatttaa aaccaaatga tacaaattcg atccgaaatc
    91621 cttggtctcc ctttgatttg aatgactcat tgattttttc acgtaattgt cgtggtgtca
    91681 atgtcgtcgt atcaatgatc acggaagctt gggctttcaa gtcgtctagt atcgcccgtt
    91741 ctttacgtat cccttcagtg atcaaaccgt ccatcgccat tggatgtgct cgtcttgttt
    91801 ctttgtagcg agccactagt tcttcgtctg aagcatctaa aaataagata gaagtgtcga
    91861 tgaagttcgt attttcgatt tcgatcagca tgttttggat ttcttcaaag aaggaacgag
    91921 aacgtaaatc cactcctaaa gccatttttg tgactttgcc tgattctttg attaattccc
    91981 agaattttgg aatcaatcga ggcggcatat tatcgataca aaaatagccc atatcttcaa
    92041 aactttggat agctactgtt ttccctgcac cgctcatacc tgttatgatg actaattgca
    92101 aattatctgt cataattacg aactcctctc acattgtgca tagtaaaatt atatcattcc
    92161 gggcgtatca ttaccagttt tctttttctt ttacaaaaag atttggacca aatatctttt
    92221 aggatatttg ccccaaatcc aattttttta cttttccgat gctgtatcca atgccttgct
    92281 tctttcagct agcgctcgtt caatcgtccg atctgctaaa atagattggg catcgatcac
    92341 tggataatgg ttatcttcag caaattcatg catcaatgac aattctgtgc aacctaagat
    92401 aactttttca cagtttaggc gctctaccgc ttcttctaag atttcgtaat atagctcttg
    92461 attcaaataa tccgattcct tgatctcatg atagatcaga taattgattt tttcttgaag
    92521 aacagtatca ggaatcactg tttcgaatcc aagatttttg acttcgcgtt catagattcc
    92581 agctttcata cttccttctg ttcctagaat cgctactctc cctgtcgtat gctgacggac
    92641 aagttcattt gctgcttctc ttggcatatg aaggatagga atatccgttg ccgcttgtaa
    92701 ttcttcaaaa aagtagtgtg cggtgttaca agtcaacaca atgaagttcg gctgcaataa
    92761 attttgtttc tcaatatcat ccagcagaaa cggcattgga ttttcttctg atcgatctaa
    92821 aatataagct gtacgatcag gaactgttgc atgattgaat aagacataat tcaaatattc
    92881 ttgatctttt gtagctttcg ttcggtggtt gatcaagcga acaaaacttt ctgtcgccat
    92941 cgtgcccatt ccgcctaaaa tactgaaaaa attctccatg aactagcctt ctttcacatg
    93001 aaaatacttt ttgaatcttg gaatatagtt atggaacata tatttcatca acaaccagcg
    93061 cttgattgac cggtcttttt cataaaacac agtagtaccg taacgatttt ctttgatcat
    93121 ttgctcagct agttttttgt cttcattttc ccgagcatat tctaaaaaga tttttttggg
    93181 cacacccatc cataatcttg ctttatctga ttgattcgtt ccataggtcg tttcaacaaa
    93241 gggtttgtta aacactcgat cttctgtgac gaaacgagcc agattcaagc cgttcaatgt
    93301 cacaaagaaa ctgctacgac cttgtctcaa attgatttca aataatttat attctccatc
    93361 tctaggatca tacttcatat cgaaattggc aaagcctgta tattcgattt tttccaaaaa
    93421 tgccttaatg gtttgataga ttttttcgtt ataatctggc atgatcacaa cgtaattacc
    93481 aatcgatgct ggtgtaggat cttctaacag tggatgacca agacacatca tccgaacttg
    93541 atgatcttca tctacatacg catttagcac acgcatatta ctatcatcac ccgggataaa
    93601 atcttgtaca atcatttcac ttttataacc ggcttcgtat atccggccta aaattaaatc
    93661 gaactcttct ctagtctcta aaataaaagc tttttttctt ccttcaaatt ggacagaaag
    93721 atactccaca ctgtttgcag gtttcaaagc aactgggaat tcaaaaggca attcctgttc
    93781 caaatgtcca tttactaaca tttcttctct gacgatcaat gttttaggat acggaagatc
    93841 atattcttca caaacttcat aaaagcttac tttattgatc agtctttcaa ataaagaata
    93901 atcaatatat ggacagataa aatattctga cagctcttgt ttatgttggg agatcaattc
    93961 tgcatatcca tcaccacaag caatcaaaag atatttttta ctcttatcag aatatctttc
    94021 ttttgccaaa cgaagcatcg tctcgataaa cacagggtct ttatcaaatc ctggaataac
    94081 ttctacattg acaattttac tgtagcgggt aggggctaac tgatccgaag catatgcttg
    94141 gcagattttg ccgtatgctt cattaaaaga acgtgccata ccataaacgt tcatgtcgct
    94201 tcctaataat atagggataa attcttcatt ttcaatactg ttcatcatta tctctcactt
    94261 actcataatc tcttgttttt cttttccaac tacacaagga atatcatacc ataaagaaaa
    94321 aagcaatcaa aggaaactcc tccgattgct caattctttt ttgaacggat tgaacttata
    94381 ggaagttttt gtctatacca atcagtgtag aaagaactac catgtaatga ttctagaaca
    94441 ctgatattaa taaaacaagc aaaaattcat ttttttatgt ttttttaact atctcagcta
    94501 tctatcctct ttataccatt atttttttca aatagtgacc tgtataactt tcttttacgt
    94561 taatgatttc ttctggtgtt ccagtcgcaa ggatcgttcc tccaccttct ccaccttctg
    94621 gtcctaaatc aatgatataa tctgctgttt tgattacatc tagattgtgt tcaatcacta
    94681 aaactgtgtt accagcatct actaatcttt gtaatacatg caacaagcgg gcgatgtcat
    94741 cgctatgaag tcctgtcgtt ggttcatcta gtatatagaa attctttcca ttagagattt
    94801 tgtgcaattc actggcaagt ttcatccgtt gtgcctcacc accggacaat gtcgttgctg
    94861 gttgccccat cgtcacataa cctaagccaa catcaacaat cgtttgcagt ttgcgatgaa
    94921 tctttggaat gtgcttgaag aattctacag catcttctac tgtcatttcc aaaatatcag
    94981 caatgctttt tcctttgtaa tgcacttcta atgtttcaga gttatatcgt ttgccatgac
    95041 atacttcaca aggaacatag acatcaggca aaaagtgcat ttcgatctta ataattccat
    95101 ccccgcgaca agcttcacaa cgaccgcctt ttacgttgaa actaaaacgc cctttcttat
    95161 aaccccgcat ttttgcctcg ttcgtttgag caaataaccc acggatatca tcaaatacac
    95221 ttgtataagt cgctggatta cttctcggcg tccggccgat tgggctttga tcgatatcga
    95281 tgatcttttc gatactttcg tagccggaaa ttgtcttgaa tttacctggc ttagcagaat
    95341 tcttatttaa tttttgcgct aacgatttct ttaagatact attgaccaat gtactctttc
    95401 cagaaccaga cacacctgtg actgctacaa attctcctaa tggaaattca acactaatat
    95461 ttttcagatt gttttctgaa gcaccagtga tcttgattgc tttcccatta cctttacggc
    95521 gttcttttgg tacagggatt gattttttcc ctgaaagata tttccctgtc aaagaatttt
    95581 catcattagc tacttcttca ggagtgcctg cagccacgat ttgtccgcct tgttctccgg
    95641 ccccaggtcc gacgtcaatc agataatctg ctgcccacat cgtatcttca tcatgttcga
    95701 ctacaacgag ggtgttgcca agatcacgca ttttctttaa tgagccaatc agtcgatcgt
    95761 tgtcgcgttg atgcaatccg atcgatggtt catctaaaat atacaaaact ccagaaagat
    95821 tcgatccaat ttgtgtagcc aatctgattc gctgcgcttc tcccccagat aatgttccgg
    95881 aagcgcgact caaagttaga tagtctagtc ccacattttc caagaatgaa agtctgtcat
    95941 ttacttcttt taaaatcggt ttggcaatta ctttctcttg ttcagataac gttaagtttg
    96001 agaagaaatc tactgctttt tgaatagaca actcatttgt ttgcccgata ttcgttccat
    96061 tgatttgaac agatagagct tggggattca atcgataacc tttacatgtc tgacaagtta
    96121 attctgtcat ataaagacgc atttgctcac gcgtgaaatc actgttcgta tcatgataac
    96181 gacgctcgat attcttcaat accccttcga aagtcgtttc tacatcgcga acgccaccaa
    96241 aatcattttc ataatggaaa tggaaaagat catctgtccc attcaagatg atatcctgat
    96301 gctcttccgg taattcttcg aatggtgtat ctaaatcgat accgaaactt tcacaagcct
    96361 gcgctaacat ttgaggataa tactgagagc tgattggatt ccatggaacg atagcacctt
    96421 gactaagtgt cttcgttcta tctggaatca ctaaatcttg atcgacttct aatttgacac
    96481 ctaaaccatc acagtctgga caagctccaa aaggcgcatt gaatgagaac agtcttggtt
    96541 ctagttcgcc aacagtaaat ccgcaataag gacaggcata gtgttcgcta aacaacattt
    96601 cttcttcacc gatcacgtca accagagcat agccgtctgc caatcgcaga gccgcctcta
    96661 aagaatcaaa tagacgagaa cgaatccctt ctttcacaat gatccggtcg atgacgatcg
    96721 cgatatcatg ttttttgttt ttctccagct caggcacttc acttaaatca tagatctcac
    96781 catcgactcg aacgcggacg tatccctctt tttggattcg ttcgaatatt tttttatgtt
    96841 gtcctttttt ctttaccacg atcggtgcca acacttggat ctttgtgcgc tcaggcaaag
    96901 ccattacttg atctgccatt tgatctactg attgactggt aatctcgata tgatcgttgg
    96961 gacaaatcgg atgtcctaca cgtgcaaaca acaagcgtaa atagtcattg atttccgtca
    97021 cagtacctac tgtcgaccga ggatttttac ttgttgtttt ctgatcaata gaaatcgccg
    97081 gactcaatcc gtcgatgcta tctacgtccg gcttatccat ttgtcctaaa aactgacgag
    97141 cataagcaga aaggctctct acgtatctcc gctgcccttc agcgtacagc gtatcaaatg
    97201 ctagggaact ttttccagaa ccggacaaac ctgtaacgac cacgaattta tctcgtggaa
    97261 tcgttacatc tatatttttc aagttatggg cacgtgcacc gtgaatcgta attttatcgt
    97321 tagccatgtt gtcctcctac tttgaagctt ttaattctaa aatcatatct cgcacattag
    97381 cggctgtttc aaaatccaat gctttcgccg catttttcat ttctttttct aatttcatca
    97441 ataacgtatc tttttcttct ttcgtcattt cttcatacga gacttgtacc gcttcttttg
    97501 tatcatcttc cgattctttc gtaatagaaa tcaaatctcg gatttcttta atgatcgttt
    97561 ttggaacgat cccatgttct tcattgtact tctgctgaat cgtacggcgt cttgatgttt
    97621 catccatcgc tagacgcata gaatctgtta ctttgtctgc gtacatgatt actttacctt
    97681 cttcattccg agcagctcgt ccaatcgttt ggactaagga acgttcacta cgtaaaaagc
    97741 cttctttatc cgcatccaag atagcaacaa gggaaacttc aggtacgtct aatccttcac
    97801 gaagcaaatt gattccaact aacacatcaa attcacctaa tcgaagatct cgaatgattt
    97861 ctgttcgttc cagcgttttg atatcactat gcaaatattt gactttgatc cctaattctt
    97921 tgaaataatc tgtgaggtct tctgccattt ttttcgtcaa tgtcgttaca aacacccgct
    97981 ggtctttttc gactcgttca tttatttcgc ctactagatc atcgatttgt cccatgattg
    98041 gacgaacttc gatcactggg tcaagcagcc ctgtcggacg gatgatttgt tggataacgg
    98101 tatctgtttg ttcatgttca tatggtcctg gcgtcgcaga gacataaatg atctgattga
    98161 catgtttttc aaactcttct aaccgaagtg gccgattgtc taaagcagat ggcaaacgga
    98221 aaccgtaatc tactagcatc tgttttctcg ctcggtcacc attatacatt cctctgactt
    98281 gaggcatggt tacgtgcgat tcgtccgcca cgatcaaaaa atcttcagga aagaaatcca
    98341 atagcgtata aggcggttca ccttctttac gtccgtccat atgccgagaa taattttcaa
    98401 ttcccgacgt gtagcccatt tctaacatca tttcaatatc gtaattggtt cgctgttcta
    98461 aacgttgagc ttccagaagt ttgttttcat ttcgcaagac agttaaacgc tgttctaatt
    98521 ccgctttgat attcgctacg gcatgctcca tatgttcatc attcgtcaca aagtgagtcg
    98581 ccgggaagat tgccacgtgt tccgtttctc ctaaaacttc acctgtcagt gcattgactt
    98641 ctcggatacg ttcgacctca tctccaaaaa actccacacg taaggcacgt tcatctcttg
    98701 aagccggaaa aatctccacg acatcaccgc gcactcgaaa acgaccgcgc tgaaaatcta
    98761 tatcatttct ttcgaattgg atactgacta agtcacgaat caactgattg cgatctagtt
    98821 ctgctccctg tcgaatcgaa actacttgtt tttggtactc aaaaggcgaa cctaatccaa
    98881 aaatacatga aacggatgca atgacgatca catcatttct ttctaataat gaactagtag
    98941 ccgaatgtcg taatttgtcg atctcatcat ttacacttga atctttttcg atatacgtat
    99001 cgcttgaagg cacataagct tcaggctgat aataatcata ataagaaaca aaatattcaa
    99061 cagcattgtt agggaaaaat tccttgaatt ccccgtacag ctgaccagcc aatgttttgt
    99121 tatgggcaat aattagtgtt ggtttattca ctttctcaat aagattggaa atcgtatatg
    99181 ttttccctgt tccagtcgca cctaataaga tttgtgcttt tttcccgccg actacgccat
    99241 ccactaattg gttaatcgct tcaggctgat caccagccgg ttggtatttc gagacaagct
    99301 caaagtgtcg ggaggtatct ctctcaatca ttcttcgtcc tccttttcat tcattccttt
    99361 tcattttacg accaataaaa agaaaaggaa agcaaaggca ttccttacta aaaaattata
    99421 ccacaccgaa catactttcg ccactttttt atcttattat gcatgttacg ttttataaca
    99481 taaaatgtta gaaactcgca tttttttcat tttttcgagc ttacagtctt gtctgattca
    99541 gcacttttcg cctataataa taaaatattg gaaatttagg aggttgatta tgaaaagaaa
    99601 gcatttattt cactcatttg ttttcttaat gacaggactt gtgacacttg cgtttggaca
    99661 tgttgcacaa gctgacaaca ctacaacggc agagtcaagc acaagcgaga caatgcaaaa
    99721 aatagagccc aaaaaagacg tatacgtaat tgccagtgac tctgcttttg ctccatttga
    99781 atttcaaaac gcaaagggcg agtacgaagg catcgatgta aaattgatgg aaaacatcgc
    99841 caaactgcaa ggatttaaca tccgaatgga ctaccgtggt ttctctgctg ctttgcaagc
    99901 tttggaatca ggacaagcag acggaatgat tgccggaatg actgtaacag atgaacgaaa
    99961 gaaaagctat gatttttctg attcttactt tgatagcggg atccaattag ctgtcaaaaa
   100021 aggagatact tcgatcaagt cttatgaaga tttgaaagga aaaacagtcg gagcaaaagt
   100081 tggtaccgaa agtgctgaat ttttaagcga ccatgaatct gaatatggct attctatcaa
   100141 atattttgat gctgctgatc aattgtacga cgggttaaaa gttggcgcaa tcgatgcaat
   100201 gatggatgat tatccggtaa tcggttatgc gatcaaacaa ggacaagaat tggaaacacc
   100261 gatcaaaaaa gaatctggcg gaaaatatgc atttgccgtt aaaaaaggac aaaatcctga
   100321 gcttcttaat atgttcaatg aaggattgaa agaattaaaa agaacaggcg aatacgataa
   100381 gattattaaa gaatatgtag cggatggttc agaaacttcc agtgagtctt ccactgctga
   100441 tgaatcaaca tttcttggac ttatccaaaa caactatcaa gcattgttga aaggactttg
   100501 gagaacaatt ctcctaactt tgatctcctt tgctttggct atagtaatcg gtgtgatttt
   100561 tggattattc agtgtagcac caatacgcgc tcttcgcaca attgctagta tttatgtcga
   100621 tatcatccga ggaattccaa tgatggttct tgcgttcttt attttctttg ggttgcctgg
   100681 tatcgttgga tttacgattc ctgacttcct tgctggtgtt atcaccttga ctcttaacgc
   100741 aagtgcttat atcgcagaga ttgttcgagg cgggatcaac gccgttcctg tcggacaaat
   100801 ggaagcttcc agaagtttag gtctttctta taatcggaca atgcaaaaaa tcattttgcc
   100861 gcaagcgata cgcatcatga tcccatcatt cgtcaatcag ttcgttatct cattaaaaga
   100921 cacaacgatc atttccgcaa tcggtgtagt cgaattgctg caaactggta agatcattgt
   100981 tgcacgtaca acacaaagta cgtatgttta cttgatcatt gcgatcatgt acttgatttt
   101041 gatcacagca ttgacaaaat tagcaaaagt tttagaaaag aaggtgaagt aagatggcag
   101101 aaaaaatctt agttgagcat ttagtcaaaa aatacggcga taacacagta ttaaacgaca
   101161 tcaatgtgtc catcaacgaa ggagatgtcg tctgtatcat tggcccttca ggttcaggaa
   101221 aaagtacatt tttgcgttgc ttgaatcgtt tagaagaacc ttctgacggt gaaattatta
   101281 ttgacggcgc ccacttgatg gataaaagca cagatatcaa cttggtgaga caacatatcg
   101341 gaatggtatt ccagcacttt aacctattcc ctcatctttc tattttggaa aacatcattt
   101401 tagcaccgat ggacctgaag aaagaatcta gagaagaagc agagaaaaaa gcaatcaaat
   101461 tgctgcagac agttggtcta gaagataaaa aagatatgta tcctgaaatg ctttccggtg
   101521 gacaaaagca gcgtgtggct attgctcgtg ctctagcgat gaaccctgac atcatgcttt
   101581 ttgatgagcc tacatctgca ctcgaccccg aaatggtcgg tgatgtcttg aacgtcatga
   101641 aaaagttagc agaacaaggt atgacgatgg ttatcgttac gcacgaaatg ggcttcgcta
   101701 aagaagtggc caatcgtgtc atgttcattg acgacggaaa ctttttagaa gacggaacgc
   101761 ctcaacaaat ctttgaaaat ccgcaaaatg aacggacaaa agatttcttg aataaagtat
   101821 taaatattta ataaaaacca aaaatgcccg gtcgcttcat gacgaccggg catttttagt
   101881 ttcttgtttt ttcacttaac tgtaccagcc gcatcgcttg ttctcctgca caaacaagca
   101941 atcgctctac ctgttccatc gcataagaaa gtaccatcgg ttcattgacg atatcaagta
   102001 catagtcgat accaccttca gaaactatga tgattatctt attctaaagc tggctttttt
   102061 gttggtacaa acatgacaac tacaaaaaa
//
LOCUS       DAZAFF010000002        86369 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000002 DAZAFF010000000
VERSION     DAZAFF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 86369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.002"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..1156
                     /locus_tag="ACG9BB_000096"
     CDS             32..1156
                     /locus_tag="ACG9BB_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN2836080.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            1181..1360
                     /locus_tag="ACG9BB_000097"
     CDS             1181..1360
                     /locus_tag="ACG9BB_000097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836081.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            1383..2300
                     /locus_tag="ACG9BB_000098"
     CDS             1383..2300
                     /locus_tag="ACG9BB_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2836082.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            2293..3126
                     /locus_tag="ACG9BB_000099"
     CDS             2293..3126
                     /locus_tag="ACG9BB_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2836083.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            3209..4327
                     /locus_tag="ACG9BB_000100"
     CDS             3209..4327
                     /locus_tag="ACG9BB_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN2836084.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            4348..6219
                     /locus_tag="ACG9BB_000101"
     CDS             4348..6219
                     /locus_tag="ACG9BB_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFN2836085.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            6213..7436
                     /locus_tag="ACG9BB_000102"
     CDS             6213..7436
                     /locus_tag="ACG9BB_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN2836086.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7522..9456)
                     /locus_tag="ACG9BB_000103"
     CDS             complement(7522..9456)
                     /locus_tag="ACG9BB_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN2836087.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            9682..10338
                     /locus_tag="ACG9BB_000104"
     CDS             9682..10338
                     /locus_tag="ACG9BB_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFN2836088.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(10412..10765)
                     /locus_tag="ACG9BB_000105"
     CDS             complement(10412..10765)
                     /locus_tag="ACG9BB_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN2836089.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            10957..11886
                     /locus_tag="ACG9BB_000106"
     CDS             10957..11886
                     /locus_tag="ACG9BB_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFN2836090.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            11897..12733
                     /locus_tag="ACG9BB_000107"
     CDS             11897..12733
                     /locus_tag="ACG9BB_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFN2836091.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            12981..13757
                     /locus_tag="ACG9BB_000108"
     CDS             12981..13757
                     /locus_tag="ACG9BB_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN2836092.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            13933..14676
                     /locus_tag="ACG9BB_000109"
     CDS             13933..14676
                     /locus_tag="ACG9BB_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2836093.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            14669..16249
                     /locus_tag="ACG9BB_000110"
     CDS             14669..16249
                     /locus_tag="ACG9BB_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFN2836094.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(16500..16979)
                     /locus_tag="ACG9BB_000111"
     CDS             complement(16500..16979)
                     /locus_tag="ACG9BB_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFN2836095.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(16995..17747)
                     /locus_tag="ACG9BB_000112"
     CDS             complement(16995..17747)
                     /locus_tag="ACG9BB_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFN2836096.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            17864..17934
                     /locus_tag="ACG9BB_000113"
     tRNA            17864..17934
                     /locus_tag="ACG9BB_000113"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17896..17898,aa:Gly,seq:ccc)
     gene            18295..19020
                     /locus_tag="ACG9BB_000114"
     CDS             18295..19020
                     /locus_tag="ACG9BB_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFN2836097.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            19275..19541
                     /locus_tag="ACG9BB_000115"
     CDS             19275..19541
                     /locus_tag="ACG9BB_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFN2836098.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            19538..19969
                     /gene="ruvX"
                     /locus_tag="ACG9BB_000116"
     CDS             19538..19969
                     /gene="ruvX"
                     /locus_tag="ACG9BB_000116"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFN2836099.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            19994..20299
                     /locus_tag="ACG9BB_000117"
     CDS             19994..20299
                     /locus_tag="ACG9BB_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFN2836100.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(20380..21525)
                     /locus_tag="ACG9BB_000118"
     CDS             complement(20380..21525)
                     /locus_tag="ACG9BB_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2836101.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(21586..22233)
                     /locus_tag="ACG9BB_000119"
     CDS             complement(21586..22233)
                     /locus_tag="ACG9BB_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2836102.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            22427..22720
                     /locus_tag="ACG9BB_000120"
     CDS             22427..22720
                     /locus_tag="ACG9BB_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836103.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            22764..23075
                     /locus_tag="ACG9BB_000121"
     CDS             22764..23075
                     /locus_tag="ACG9BB_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836104.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            23072..23696
                     /locus_tag="ACG9BB_000122"
                     /pseudo
     CDS             23072..23696
                     /locus_tag="ACG9BB_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            23837..23995
                     /locus_tag="ACG9BB_000123"
     CDS             23837..23995
                     /locus_tag="ACG9BB_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836105.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            23996..24358
                     /locus_tag="ACG9BB_000124"
     CDS             23996..24358
                     /locus_tag="ACG9BB_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836106.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            24395..25243
                     /locus_tag="ACG9BB_000125"
     CDS             24395..25243
                     /locus_tag="ACG9BB_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFN2836107.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            25233..26708
                     /locus_tag="ACG9BB_000126"
     CDS             25233..26708
                     /locus_tag="ACG9BB_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFN2836108.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            26974..27381
                     /locus_tag="ACG9BB_000127"
     CDS             26974..27381
                     /locus_tag="ACG9BB_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFN2836109.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            27384..27599
                     /locus_tag="ACG9BB_000128"
     CDS             27384..27599
                     /locus_tag="ACG9BB_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836110.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            27603..27983
                     /locus_tag="ACG9BB_000129"
     CDS             27603..27983
                     /locus_tag="ACG9BB_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN2836111.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            28117..28272
                     /locus_tag="ACG9BB_000130"
     CDS             28117..28272
                     /locus_tag="ACG9BB_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFN2836112.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            28341..28814
                     /locus_tag="ACG9BB_000131"
     CDS             28341..28814
                     /locus_tag="ACG9BB_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFN2836113.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            28811..30505
                     /locus_tag="ACG9BB_000132"
     CDS             28811..30505
                     /locus_tag="ACG9BB_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN2836114.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            30471..30656
                     /locus_tag="ACG9BB_000133"
     CDS             30471..30656
                     /locus_tag="ACG9BB_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836115.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            30660..31835
                     /locus_tag="ACG9BB_000134"
     CDS             30660..31835
                     /locus_tag="ACG9BB_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN2836116.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            31828..33351
                     /locus_tag="ACG9BB_000135"
     CDS             31828..33351
                     /locus_tag="ACG9BB_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN2836117.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            33407..33691
                     /locus_tag="ACG9BB_000136"
     CDS             33407..33691
                     /locus_tag="ACG9BB_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN2836118.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            33678..34013
                     /locus_tag="ACG9BB_000137"
     CDS             33678..34013
                     /locus_tag="ACG9BB_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN2836119.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            34308..34934
                     /locus_tag="ACG9BB_000138"
     CDS             34308..34934
                     /locus_tag="ACG9BB_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836120.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            35676..36512
                     /locus_tag="ACG9BB_000139"
     CDS             35676..36512
                     /locus_tag="ACG9BB_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFN2836121.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            36576..37007
                     /locus_tag="ACG9BB_000140"
     CDS             36576..37007
                     /locus_tag="ACG9BB_000140"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2836122.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(37069..38391)
                     /locus_tag="ACG9BB_000141"
     CDS             complement(37069..38391)
                     /locus_tag="ACG9BB_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFN2836123.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(38545..40185)
                     /locus_tag="ACG9BB_000142"
     CDS             complement(38545..40185)
                     /locus_tag="ACG9BB_000142"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFN2836124.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            40518..40988
                     /locus_tag="ACG9BB_000143"
     CDS             40518..40988
                     /locus_tag="ACG9BB_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFN2836125.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            41210..42313
                     /locus_tag="ACG9BB_000144"
     CDS             41210..42313
                     /locus_tag="ACG9BB_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFN2836126.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            42597..43277
                     /locus_tag="ACG9BB_000145"
     CDS             42597..43277
                     /locus_tag="ACG9BB_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN2836127.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            43367..44401
                     /locus_tag="ACG9BB_000146"
     CDS             43367..44401
                     /locus_tag="ACG9BB_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFN2836128.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            44415..44750
                     /locus_tag="ACG9BB_000147"
     CDS             44415..44750
                     /locus_tag="ACG9BB_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836129.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            44734..47214
                     /locus_tag="ACG9BB_000148"
     CDS             44734..47214
                     /locus_tag="ACG9BB_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836130.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            47270..48829
                     /locus_tag="ACG9BB_000149"
     CDS             47270..48829
                     /locus_tag="ACG9BB_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2836131.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            48826..50190
                     /locus_tag="ACG9BB_000150"
     CDS             48826..50190
                     /locus_tag="ACG9BB_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFN2836132.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            50361..51431
                     /locus_tag="ACG9BB_000151"
     CDS             50361..51431
                     /locus_tag="ACG9BB_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2836133.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            51446..52114
                     /locus_tag="ACG9BB_000152"
     CDS             51446..52114
                     /locus_tag="ACG9BB_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2836134.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            52134..52712
                     /locus_tag="ACG9BB_000153"
     CDS             52134..52712
                     /locus_tag="ACG9BB_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2836135.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            53113..54024
                     /locus_tag="ACG9BB_000154"
     CDS             53113..54024
                     /locus_tag="ACG9BB_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN2836136.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(54402..55319)
                     /gene="rnhC"
                     /locus_tag="ACG9BB_000155"
     CDS             complement(54402..55319)
                     /gene="rnhC"
                     /locus_tag="ACG9BB_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFN2836137.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            55521..55958
                     /gene="zapA"
                     /locus_tag="ACG9BB_000156"
     CDS             55521..55958
                     /gene="zapA"
                     /locus_tag="ACG9BB_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFN2836138.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            56016..56564
                     /locus_tag="ACG9BB_000157"
     CDS             56016..56564
                     /locus_tag="ACG9BB_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFN2836139.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            56679..59039
                     /locus_tag="ACG9BB_000158"
     CDS             56679..59039
                     /locus_tag="ACG9BB_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFN2836140.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            59126..59440
                     /gene="trxA"
                     /locus_tag="ACG9BB_000159"
     CDS             59126..59440
                     /gene="trxA"
                     /locus_tag="ACG9BB_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFN2836141.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            59622..61400
                     /gene="uvrC"
                     /locus_tag="ACG9BB_000160"
     CDS             59622..61400
                     /gene="uvrC"
                     /locus_tag="ACG9BB_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFN2836142.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            61545..62444
                     /locus_tag="ACG9BB_000161"
     CDS             61545..62444
                     /locus_tag="ACG9BB_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2836143.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            62437..63699
                     /locus_tag="ACG9BB_000162"
     CDS             62437..63699
                     /locus_tag="ACG9BB_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2836144.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            63860..65758
                     /locus_tag="ACG9BB_000163"
     CDS             63860..65758
                     /locus_tag="ACG9BB_000163"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFN2836145.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(66088..66807)
                     /locus_tag="ACG9BB_000164"
     CDS             complement(66088..66807)
                     /locus_tag="ACG9BB_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFN2836146.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    66997..67044
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            67144..67476
                     /locus_tag="ACG9BB_000165"
     CDS             67144..67476
                     /locus_tag="ACG9BB_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836147.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            67463..69466
                     /locus_tag="ACG9BB_000166"
     CDS             67463..69466
                     /locus_tag="ACG9BB_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFN2836148.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            69485..69955
                     /locus_tag="ACG9BB_000167"
     CDS             69485..69955
                     /locus_tag="ACG9BB_000167"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFN2836149.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            69976..70560
                     /locus_tag="ACG9BB_000168"
     CDS             69976..70560
                     /locus_tag="ACG9BB_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFN2836150.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            70575..71561
                     /locus_tag="ACG9BB_000169"
     CDS             70575..71561
                     /locus_tag="ACG9BB_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFN2836151.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            71551..71862
                     /locus_tag="ACG9BB_000170"
     CDS             71551..71862
                     /locus_tag="ACG9BB_000170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFN2836152.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            71884..73665
                     /locus_tag="ACG9BB_000171"
     CDS             71884..73665
                     /locus_tag="ACG9BB_000171"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFN2836153.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            73658..75034
                     /locus_tag="ACG9BB_000172"
     CDS             73658..75034
                     /locus_tag="ACG9BB_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFN2836154.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            75041..75676
                     /locus_tag="ACG9BB_000173"
     CDS             75041..75676
                     /locus_tag="ACG9BB_000173"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFN2836155.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            75706..77061
                     /locus_tag="ACG9BB_000174"
     CDS             75706..77061
                     /locus_tag="ACG9BB_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFN2836156.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(77253..78647)
                     /locus_tag="ACG9BB_000175"
     CDS             complement(77253..78647)
                     /locus_tag="ACG9BB_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFN2836157.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            78739..79710
                     /locus_tag="ACG9BB_000176"
     CDS             78739..79710
                     /locus_tag="ACG9BB_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFN2836158.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            79707..80489
                     /locus_tag="ACG9BB_000177"
     CDS             79707..80489
                     /locus_tag="ACG9BB_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFN2836159.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(80658..81029)
                     /locus_tag="ACG9BB_000178"
     CDS             complement(80658..81029)
                     /locus_tag="ACG9BB_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN2836160.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            81163..81606
                     /locus_tag="ACG9BB_000179"
     CDS             81163..81606
                     /locus_tag="ACG9BB_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFN2836161.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(81649..82458)
                     /gene="yidA"
                     /locus_tag="ACG9BB_000180"
     CDS             complement(81649..82458)
                     /gene="yidA"
                     /locus_tag="ACG9BB_000180"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFN2836162.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(82477..83844)
                     /locus_tag="ACG9BB_000181"
     CDS             complement(82477..83844)
                     /locus_tag="ACG9BB_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN2836163.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(83828..84658)
                     /locus_tag="ACG9BB_000182"
     CDS             complement(83828..84658)
                     /locus_tag="ACG9BB_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFN2836164.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            84821..85258
                     /locus_tag="ACG9BB_000183"
     CDS             84821..85258
                     /locus_tag="ACG9BB_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFN2836165.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            85334..85954
                     /gene="rpoE"
                     /locus_tag="ACG9BB_000184"
     CDS             85334..85954
                     /gene="rpoE"
                     /locus_tag="ACG9BB_000184"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFN2836166.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(86117..>86369)
                     /locus_tag="ACG9BB_000185"
     CDS             complement(86117..>86369)
                     /locus_tag="ACG9BB_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN2836167.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT        28439 a        14614 c        17919 g        25397 t
ORIGIN      
        1 ataagaactt gaaaagaaag aagggataga catgaaaaaa agatacaaat tggtgatagc
       61 tttgctaaac attaccctat tattcagctt aagtgcatgt ggaaaaacta acgaagaagt
      121 aacagcaaac atcgcagatc caatgataaa aaaagccact acattagaag tatatgtcgc
      181 aaacggcaaa ctgacaaact atgttgaaaa tgctgcaaaa atctataacc gcaaaactgg
      241 agcaaatatt gacttaaaaa ttgtgaatgt agcttcggga acagcaacag tacaaatgat
      301 caccccaaaa ttggtaacac acgaagatat gccggatatt atctccatgg cagacacttc
      361 agcagcaggt gtgctggaaa aatttcaaga tgctttttat agtgctgatg aatatggatt
      421 ttatgacaaa tatggcgatc agttttatga tcaaaaattg aatattttgc gtcaacaaac
      481 gagtaatgaa acgattattc cttgggctag tgattttacc ccagcacttt gttattatca
      541 accaaaaaaa tttgctgaaa taggtgtaga ttttgaagaa atcagttctt gggaagaata
      601 tatccaggtg gcaaaagcat taaaagaaaa aactggtagt tttggaattg ccttgccaga
      661 atctggggac caagaattgt ttatcaacat gctagcccag cagaatcaat ctttgcttga
      721 tgaagaagga aatatccgtc tgaatactaa agaagcgaaa catgctgcaa agttaatcaa
      781 agaaatgatc gactcagata ttgtacattt ctacggtgca caagatgctg agaaagcatt
      841 tcaagaatct gcaatgtttg ttgctggcgg ctggtatgct acgaatatgt ctttgaattt
      901 tcctgatgca tctgacaaat ggcgcatggc tccactgatt cctttttcaa aagaaaatcc
      961 aggaaaaggt gcagtatcag gaggatcaag tttttatgtc cctaaagatg caaaaaatcc
     1021 acaagtggca caacaatttc ttacatttat gctgacagat gaagaatgct tagccaacgc
     1081 actggagctg ggcgtggcta catctaactc attggcctat caaacagaag cagctgagaa
     1141 aaagtttgac tactaaggag gacaaaaata ctatgaacta ttgaataaaa tcaatgaaaa
     1201 tacatcaaat actgttttac cagccactta ttcagatgct acgtcttatt tgactaccgc
     1261 tgcatataac tattggcaaa caggtgattt tgaaaattca tatctaaaag aagcagaaaa
     1321 ttttgctcat aagtacggcg taaaattaac tggtgattaa aaaatattag gaggacttaa
     1381 cgatgatgca atcttcgtta ccaagcaaga caataaagaa cagcaagaaa aaagcaacag
     1441 atacgtctca taaaaaaagt gcttatggat tggtcacacc ttttgttatt ttctttattg
     1501 tgacgatttt gattcccatc attatctcgt ttgcttttag ttttatgaaa attggctact
     1561 catttgaatt tgttggattt gacaattatc tagctatctt ccgagatcca ttgtttatta
     1621 aatcttacac gaatgttttg attatcatgg caggatcaat tccaataacg atgctattag
     1681 ctatttactt tgctgtttta ttgaattcag cacagttaaa aggaaaagga ttttttagaa
     1741 ctgtctatta tattccaacc attacatcaa cggtggctgt agcttccgta ttcatgactt
     1801 ttttcaatcc gactgggtta ttcaatggat ttttgaatgc gctaggaatt aacggtgtac
     1861 agtggttaac aaatcctttt tggatacgaa tatcaatgat catcacgatg atttggctta
     1921 gtgtaggata taacactatt ttatttttag ctggtcttca agggatttct aaagagatat
     1981 acgaaagtgc tgaaatagat ggagcatcga aatttcggca attcttttta ttaacgatcc
     2041 ccatgctaaa gccaatcatt ttaatggcca cagttttagc cactatcaat ggcttgggta
     2101 atttcaatat cccaaatata ttctttggga cttccaatgg accagaaaat tcagctttgg
     2161 tcgtaggagt caatttatat aaaacaagtt ttgaaatggt tgattttgga aaagcctctg
     2221 ctattgcctg gacgatggtg tttgtatctg ttatattatc ggctattcaa tttaaattcg
     2281 gaggtgataa aaatgactaa acaaaagata caaagcagaa tttttacgta cgtcattatt
     2341 gtgtttggtg ccttaatatt tttgatgcca tatttatata tgattgtagc ttcaacgcaa
     2401 agtaatcaag aaattttaag tggcggaatc aatttcaagt ttggggatat gctattaaaa
     2461 aactggcaag ccatcatggc gagatataat tatcccttga ttatttggaa ttcgattgtt
     2521 attgctgtaa tcagtacagt tttagctaca gccagttcta caatggcggg ttatgcatta
     2581 gcaaagtatc gttttatggg gaataaactg ttgttcaata cagttatgct atctaggatg
     2641 gtgccttttt ttaccacatt gattccttta ttttacatta tgagtcgttt aggattatcg
     2701 aatacttatg caggtgtgat catcccttcg attgccagta caacttcagt gtttatgatg
     2761 cgacaatata gcagtcaatt tcctacagca ctgatggagg ctgccagaat cgatggtgcc
     2821 aatgagtggg tgatttttac gaaaatcgct gtaccgattt tatcacctac gattattact
     2881 agtggtttac ttatctttat gggaagttgg aatcaatatc tatttccatt agtcatgctt
     2941 tcagataaag ataaatatac ggttccctta attatcaaaa atatgacggt ttctagtaca
     3001 ggtgagccaa ttaattatgg cgcaattatg ctaattttag caacttcagt gatcccaatg
     3061 gtcttgattt atgtgtgggc gcagtctaaa tttaaggaga atgacattgg agctgcaaat
     3121 aagtaaaaaa aataacgaat gttaaccatc tagttcgttc taggagcaac tccacattca
     3181 taaaaattaa aagaatggcg ggatatccat gaatgaacag acgaaggaat ggtcaagaaa
     3241 aattttgaat gtagcttctg aaaagataga tgcggaatta gacagaatgg ggaataaaat
     3301 tccatatatt tcttacaacg gaaaatatac agacgtaata aaggacagag gaatcgactg
     3361 gtggaccaat ggtttttgga gtggtatttt atggcagctc tttcacaaga caaaacgaga
     3421 aaagttcaaa gaggcagcaa tacaacaaga acaattatta gaatctgccc tgtaccattt
     3481 ttcagacgtt catcatgatg taggtttttt attcttacca tcggctattg cacattatca
     3541 acagtctcaa aacaaacata gtcgacaact agggttacat gcagctacgg tacttgctgg
     3601 acgctataat atttcaggga actatttagt agcatggaat gaccataaac ctggttggat
     3661 gattattgat agtatgatga atttaccatt actgtattgg gcttcgaggg aaactggtga
     3721 tccgaggttc tctaaaattg ctcggcagca tgcagataca acagctaagt atctagtgag
     3781 gaaagatgga tcaaccgggc atatcgcaga atttgatgca gaatcaggga aatatattcg
     3841 gttacttggc gggcaaggat acaatgagaa ttcttgttgg agcagaggaa atgcatgggc
     3901 tttatatgga ttcgcgttaa gttatcgtta cacaaaaaat ccgtactatc tagctatagc
     3961 agaaaagata gcagtaaatt ttattcagaa aatcgaaaaa actggttata ttcctctcat
     4021 tgattttgct gtaaaagatt cgaatattga taccagtgct ggagtatgcg ctgcatgcgg
     4081 tcttctggaa ttagcaggat atgtttcctc tgtacgatca aaaatgtatc atgaagctgc
     4141 tatcaatata ctccaaaaga cagaaaagca ctatgcaaat tggaatatgg atgaagatgg
     4201 gatcattgga ggcggagcag aagcatacca tagagaactg acttataatg tgccactgat
     4261 ctattctgat tattttttta ttgaaggctt acttcgatta gaacagtcgg aattagctat
     4321 ttggtagtaa aaagaggaga aaatacaatg aaacatccag taccactatt aaggaccaga
     4381 caggattact ttgacttatt taaaaagata aatcaaccac tccagccgtt ttatcgagca
     4441 agtggcgcac aaattgatta tatacatcaa ggggtaggat atggaaataa aattgcagga
     4501 atggaaggat tctctcgggt tttatgggga gcggggcccg caattgacca attagatgaa
     4561 cgatggttga aattaattct atcaggaata aaagctggaa cagatccttc ccatcccgat
     4621 tattggggag aaatacatga aagagaccag cgtatggtag aaatgcccgc aatgttatta
     4681 gcgttatatc atcatgaaca attattatgg aaaaaattag atacaggaga aaaaagccag
     4741 attattcatt ggttctcgca aatttttgaa tatgaatgtg cagatggtaa ttggcaattt
     4801 tttaagataa ttgtcggaac aattatccaa aaatttggtt atccagttaa agaatcagca
     4861 ctgaaagaag catttgataa tatagaaaat tgttatcttc aaaatggctg gtatcgagac
     4921 agttcaagag gtcgccaaga ttattataat ccttttgcat ttcattatta tggtttgatt
     4981 tatagcgtac tagaaccaga aaagcccatt agccagatat ataagaaccg agctattgat
     5041 ttttctagag actatattca tttttttgcg gaagatggtg caaatgtgcc ttttggtcga
     5101 agcatgattt atcgttttgc agtttcaagc ttttggtcag ctatgttgtg ggccgatctt
     5161 ttgcctttta aattaggtga aatcaagggg ttagtcaatc ggaatataag atggtggatg
     5221 caaaaagaaa tatttgatga aaaaggtatc ctgaatattg gttataccta ccctcaattg
     5281 acattgactg aaccctataa tagtacgtta tctccattat ggctgaataa gattttttta
     5341 ttgttggcgc tacctgaaga ccatgagtat tggacggcta aagaaatgcc tatgcctatt
     5401 cgtgaaggaa ctaaacttct ttcagaagcc aatttgctca tacaacatga taatggacat
     5461 acattttttt tgaatgcggg acaattaggg ccaaatttcc atacattgac caatgaaaaa
     5521 tatttaaaat ttgcttattc tagtcaattt ggtttttcga taccgcgagc gaatcaattg
     5581 aaagcagaaa tggcaatgga ttcaatgcta gggattcagc gagcggacac atatatcacg
     5641 accagtttta agaatgagag catcaaaacc tatggacagt tcatcgttcg aaataatgta
     5701 cgagacattg tatgtaagaa agaggtactt gcctctacat gggaacctac tttacaggca
     5761 aaaatcagaa cttggcttat tccttttgaa ggctggcaga ttcgcgtaca caaagtagaa
     5821 ttagatacag aatatgttct atatgagaca gggtttgcta ttgaatgttc tcctgagaaa
     5881 gaaggaataa tcatagaaga agacaaggaa aactataggg tgagtgaagc tggcttttca
     5941 ggtattatct gtttaagtga cgatactatt aaacgaaaat ccacagctat tatgggtttg
     6001 cccaatacaa acttgatgac atgggaaaac acttttattc ctggattaga gggtacattt
     6061 ggaaaaggaa cccattggct cggaacagga gtggttgctc atcaagatcg ggattatagt
     6121 ttagaagtat ggaacaatcg tcccaaaatt gattttgatg ggacaaccgg gctaactatc
     6181 caaaataaaa acaataaaaa aagactaaaa ttatgctaaa ggctggtggg gaaatgcttg
     6241 ataaagtgat gtttgaagag ataatacatc cagaaaaatg gataaatgaa tgttataaag
     6301 aattaatgga aaaagaatgg aataatgaga tcgagcgttg tcgcagacac acggagaggt
     6361 taatcaaacg tatcagtaac agtcagtatc aattcccaag cgagtgtcct ataaaaggac
     6421 gttatccatt aagagacaat attggttgga cagaaggatt ttggacagga attaattggt
     6481 tgttatttga gaagacgggt gatcctttat ttcaacaaac agctgaaaaa gcagatatag
     6541 attttatcca caggattgac tgtcaaatta tcactaatca tcacgatctt ggttttttgt
     6601 attctttatc aaccgtagca gattataaaa taacgggaaa tcgaaaaatg aaggaagctt
     6661 caattgatgc tgcagaaaca ttacttcagc gtttttggga gaaggcagga gttatacaag
     6721 cttggggaga tcctagtgat ccttcacaac aaggtagaat gatcatggat tgttgcatga
     6781 atcttccatt attgtattgg gcgagtgaag aaactggaaa tcaaaaatat aaaaaagctg
     6841 ctcaggctca tattcaaaat gcagcagatt atttagtaag agaagatggt tcaacttatc
     6901 atacatttta tatggatgtc aaagatggaa aaccaagata tggaagtacg catcaaggtt
     6961 atagtgataa ttcctgttgg gctagaggcc aagcatgggg gatttacggt tttgcattaa
     7021 gttatcgtta tttgaaagaa caaaaatatt ttgatttaag caaaatatta gccaattatt
     7081 ttatgaatcg gttgccagaa gataccattt gttattggga tttgatcttt gatgattcta
     7141 gcagagaaga aaaagataca tctagtatgg tgatcatggt ttgtggatta ttagaattaa
     7201 tagaatcagg tctattagac aaaacagaaa agaacttata ccagtctgct gtttggaaaa
     7261 ttatgcgaca attgaaagaa agatatgcta caacggaaga agaagatggc tttttaacac
     7321 agtctgttta taataagccc aaaaataatg gggtaaatac accttgtatt tggggtgatt
     7381 actattattt agaagcttta gtgagaatga ctagtaattg gtcttcttat tggtgaaaaa
     7441 ggtaaatatg gaatttttgt ttgataaaat aaaaaaagaa aaaccaaaac agcatgaatg
     7501 gtttctgttt tggttttttc gttaattttc atattcttct aattccaagc ttagttcttc
     7561 ccattccgtc aattttgttt cttgatcttg tctagcagct tctaaatctt ggttcagctc
     7621 attgagtttt acgtgatctt ccaggttttc tggcgcgacc atttcttgtt ccaactgatc
     7681 gatccattca tcgatccgcg ccatttcttc ttcaacctga gtaatttttc gttgaaggct
     7741 acggataagc ttttgctgtt ctttgctttg gtaataatca gatttgttcg tgggagcagg
     7801 agcagattct ggctcatctt gtgccaacaa ttcggcgatt tcttcttctt cctgcttttt
     7861 ctctaaataa taatcatagt ctcctagata gactttgctg ccattttcag agagttcgac
     7921 gacttttgtc gcgatccgat tgatgaaata tcggtcatgg gaaacaaaaa gcagtgtgcc
     7981 gtcataatcg atcaatgcat tttctaatac ttctttgttg tcaatatcta aatggtttgt
     8041 tggttcatcc aaaatcaaaa aattttcttt gttcatcgct aacttggcta aagcaacccg
     8101 tgctttttca ccgccactta gcagagggat tggtttttct acatcttcgc cagaaaataa
     8161 gaaagcacct aaagcacttc ggatctcttt ttctggagtg gtaggatgct catcccacaa
     8221 ttctgctagg atcgtcttgt ttgtatgcag atcagcttga ccttgatcgt aatagccgat
     8281 ggtgacattt gtccctagcg tttcttttcc acggataaaa gggatctggt gaatgattga
     8341 tttcagcaac gtcgatttcc cgataccatt cggacctact agagcaatcg cttcttgacg
     8401 acggatatct agattgattg gttcagataa ggtgacatta tcatatccga tagcggcatc
     8461 ttcgatttgc aagactacat ttccagaagg cttttctata ctgaatagaa agtttgcttg
     8521 tttttcatcc ccttgaggac gatccagccg gtccattttt tctaatgtct ttctgcggct
     8581 ttgcgctcgt tttgtggtag aggcgcgaac aaggttccga gcaacgaagt cttctaattt
     8641 atggatttct gtttgctgct tttcataagc cttccattca cttgctaact gttctgcttt
     8701 caaatctaga tatttcgaat agttaccttt gtaatggcgc atttttcttc tgcttaattc
     8761 atagacttca ttgactactt tatctaaaaa ataacggtca tgggaaacaa tcaataatgc
     8821 accagtatag ctttgcagat aattctcaag ccaggaaagt gtctcgatat ctaaatggtt
     8881 cgttggttca tctagaatca aaatgtcagg gtgttgcaaa agcatgcgag ctaaagctaa
     8941 acgtgtgcgc tgtccgcctg ataaagtatc gattttctga tcataaaatg aagaatcgaa
     9001 tttaaaccca tgcaatacgg aacgaatttc attttcataa ccatagccgt ttcgatctga
     9061 aaaatcgtgt tgcagccggt cgtattcttt taaataagaa ctataagcag gtgtgtctgg
     9121 cgtaccttcg ctaatgaaaa tctctagctc gcgcatccgc tcttccattt gtctaacttg
     9181 ttcaaaagct ttcagcatct cgttccaaac tgtttcattg gaatcaagtc ctgtgtcctg
     9241 agcgaggtat ccgagagtcg ctgttttatt tttagcgatc tttccttcat caggtgcttc
     9301 aattccagca atgatcttta ataaagtcga ttttccagcg ccatttctgc caactaaagc
     9361 gacacgagcg cctgtactga tttctaaatg tatgttttca aataagactt ctgcaccaaa
     9421 atggcgagca atctgattgg cttgtaataa tatcatggaa tcctcttttc tactttgtga
     9481 attctcctca aataaaaaac tatttttcaa ttagtagtct accataaatt ccattatact
     9541 gcgagatttg cagaaatgat aaataaatga ataaagaata aaatttcaca atatctgagc
     9601 aatggaattg attttttcct ataaatactg ctatgatgga gaaatcaaag atgtaattag
     9661 acaatacgtg gaggtcattt cgtgaaagat catattattc caaaggcgac agctaaaaga
     9721 ctgcctttgt attatcgcta tttaaggatt ttaaataatg ctggaaagaa aaaagtgtct
     9781 tcaactgaat taagtgaagc cgttcaagta gatagtgcaa cgatccgtcg agatttttct
     9841 tattttggcg aattaggaaa acgtgggtat ggttatgatg tagaagatct tatgcacttt
     9901 tttgcaaaga cattaaacga cgatcaattg acaaatgttg cgctgatcgg tgtagggaac
     9961 ttgggaagtg ctttactaaa atacaaattc catcaaagta atagcatacg tatcagttgt
    10021 gcttttgatg tgaatgagga aatcgttggg cgcatcgtag acggtatccc cgtttatcca
    10081 atggaagata tgtcagaaca aatcaagatc caacaaatcg aagtagcaat tttaacgata
    10141 cccgctgaaa gagcacaaga agtagtcaat cagttaacgg aagcaggaat caaaggaatc
    10201 ttgaacttta ccgctgctcg attgaatgct ccgtcagatg tattgatcca aaatgttgat
    10261 ttgacgaatg aattgcaaac cttgatctat ttcttgcatt ctgacgcaaa tgcgcattcc
    10321 gaaaatacag cagaataaaa ataaaaagcg attgaacaaa tgaaggcaaa actttcactt
    10381 gttcaatcgc ttttttctgt ttggacaacg attattttgt tcggaagatt tcttcataag
    10441 aaagggaatc atcttctgct gtcagttcat ctagtagata acggattttt ttaaagtcga
    10501 tgaaccgtgc ttgccgaaag acttcttttc cttgatgaaa aatcaggaca actggcgcag
    10561 tcagcacttc aaatttacta gctacttctg gtgtttcaaa tgcatccaaa tgaagtgccg
    10621 gaatatcata atgtgtgaga agttcctcta gtcgtggacg aacagcgtga caaacactgc
    10681 aattaggcat cgaaagataa atgacggcaa cggatgactg ttgcagtatg tcttgaactt
    10741 cagtcaagtt agtggtttgt ttcattttta ttctcccttt tatgatagaa gtgatatgta
    10801 tgagacctta atttaatggg aataacagaa aaagtcaatt gaagtgcttt ggtaaaaaat
    10861 aagagttgtg taaaaattta cagagcaact tgtttttttt gaataaaagc gtatatttgc
    10921 aaagcgaatg aactagaaat aaaaaggagg aaactcatgt tttcttttat tccaaattcg
    10981 gttttgcaga tgagtacgat tttcttatcg atcgttattg aagcattacc tttcgtatta
    11041 ttaggctgtc ttatctcagg cgccttacaa gtatttttga ccccagaacg tgtgaagaga
    11101 tggcttcctg aaaatcgtat cttctctatt tttactggtt cgatcttagg atttttcttt
    11161 ccttcatgtg aatgcggcat cgtaccgatc gttcatcaat ttgttaaaaa aggcgtacca
    11221 gttcacacag cgtttgcttt tatgctgact gctccgatca tcaatccaat cgtgattttt
    11281 tccactttta tcgcatttgg caattcttgg cagatggcaa gctggcgggt gataggcagc
    11341 ttttttgttg cccttgtgat tggtgtctgg cttgcctatt ttcaaaaaga cgctgtattg
    11401 aaagaaaaaa tcacgtctaa agtagcagaa aatcaccatc atcatgtcca tgaagaagaa
    11461 caaagtgaga agaatgaacc aaaaaatcag ctgatccatc aaactgggca tgtgttgaca
    11521 cattcaatcg acgaattttt tgatacaggt cgttatttga tcattggcgg attaatcgca
    11581 gctgcgatgc agacttattt accaactaga attatgctaa cgctcggatc aacaaagatt
    11641 ttagccatta tcatcatgct gttattggcc tttacgatgt ctctttgctc agaagcagat
    11701 gcgtttatag gatcttcttt gttaagtctg ttcggcacag cacctgtagc tgcctttttg
    11761 gtctttggcc caatggtcga tatcaaaaat ctactgatga tgaaacgata ttttacagga
    11821 cgctttattt tatcgctgat cggactgatt gcccttagcg ttattggatt cacattattt
    11881 atttaggagg gaaaatatgc tgcggttttt gattttatct gggtatttcg aattgatgat
    11941 gtacttgcaa gtttctggca aactcgatca atatatcaat gtccattatc gctacttagc
    12001 tattctttca atgattttat ctgctctttt ggctttagtt cagctgtatt tgtgggtcaa
    12061 aaacggaaaa gcagaagaaa aggcacacga gcacgatcat gaccatgggt tgtcaaaacc
    12121 ttatcaacgt ggaatcgctt atgtactgct agctttgcca gtaatcgtgg ggacgttgtt
    12181 tccaacagtt agtctcgaca ctacgatcgt agaagccaaa ggatttaatt tcccagtaag
    12241 taaagaatca gtaggggatc cggagatgca gacacaatac ttaaagccgg ataccagtat
    12301 ttatttcaac aaatcagact acttagacca aatgaatgag ttgaaagaaa agtatgggga
    12361 taagcaaact atcaagatca cagatgaaaa ctatttagaa gttatggagc taatttataa
    12421 ttaccctagc gaattcattg gcaaaaaaat cagctatgaa ggatttgttt accaaacacc
    12481 gaacgatagc aatacagata tcttcttgtt ccgattcggc attatccatt gtgtggcaga
    12541 ttcaggagtc tttggtttgt tgacgcatat gccggaagga gtaacggtta aaaatgatga
    12601 atggtataaa atagaaggaa ccatccaatc agactattat caaccattca agcgtgaaat
    12661 cccatctgta gttgttacaa acgctgagaa agtctctgct ccgaaaaatc agtatgtgta
    12721 tcgttctttt taaaagaaca aaataaataa acatagctca gacagctgac acgcaaggac
    12781 gtcggttgtc tggctttttt tgcatgatgt attcatttgt ttgatattta ttttagaagc
    12841 agcatcctga aagtatttga tatcggtttc aaaggtgttg gaaaagattg tgaagagaat
    12901 tacaatttat tcctaacatg tatgttgaaa agttgtatga aatgattttg atagtaaaat
    12961 atcgagtgga ggtacttttt atggtaacgc gtatatttat tttggatgat agtttggttt
    13021 ttgaaaaaat gctagtagaa attttggaag aatccagaat tttattaagt ccttggaaat
    13081 ttgagatcgt tcagggattg aacgttatac aatttgttga attcattaaa aataatgaaa
    13141 tctattcctc agatattttt ttcctagaca tcgatttgaa cgcctattat agcggtatcg
    13201 aactaggcga aatgcttaga gaaaagaatg aaaagtgtaa aattgtttat ctaacgtctc
    13261 atccgaataa agcaataact gttattaatc gaaagattac gcctagtggt tatttgataa
    13321 aagaattcac ttatgaaaaa aataaaaaaa gcatccaaag ttacttattg aaagaaaaag
    13381 aaaactatcc tgttcaacat gctacgaaaa aaaggtggac aactgtccgt tatggtaata
    13441 gcgatcacta tatccattac gatgaaatcc tttatctaat gagcatgcca ggttataaaa
    13501 ataaaatagg cctagtattg aaagatgaag agattctcgt aaacggcaca attaaaaaat
    13561 ataaaaaatc actcgaatat gagtatttat gtaaagattt aaaagcttat atcattaata
    13621 ctgaaaacat tacatctatt agcagagtaa acgaaatagt aacatttgca aatggagaac
    13681 aactagagat gggaaagaga agtattgata aactaaagaa ttttatgcaa gaaaagatag
    13741 aaaatgaatc attttaaaga aaacaaagac tcatatctta cctgttagaa gagtgttttc
    13801 ctgtagaaga gaacaaccca agtagcagcc tagtcgtttc tatccttttt tgttatgcac
    13861 tctttttgac atttactcca ccaataacga agggtacaat aaaaagtaat aacaaacaaa
    13921 aggagtacag aaatgattga attcatcaat gtaagtaaaa gttacgggaa aaaacaagca
    13981 ttaaaagact tgtctctctc gataagacaa ggggaaattt ttggattttt aggacataat
    14041 ggtgccggaa aatcaactac gattaaaagt ttagttagta ttattgaacc atcatcggga
    14101 acgattttag tagatggaat gaaattgacg gaaaatcgat tgagtatcaa acaaaaaatc
    14161 ggttatgtgc ctgattcccc agatattttt ttacaattga cggctggaga atattgggac
    14221 ttgatttcag ctgcctatga attagacaca caaaaaaaag aaaagcgttt agcagaattg
    14281 acagcgcttt ttgatatgta tagtcatcaa aatgaaacaa tcgcaagttt ttcacatgga
    14341 atgcgtcaaa aaacgatttt gatcggcacg ttgctgccag atccagatat ttgggtattg
    14401 gatgaacctt tgcaaggact tgatcctcaa gcagcatttg atttgaaaga aatgatgaag
    14461 gctcatgccg caaaaggaaa aacagtcatt ttttctacac atgtattgga taccgcacaa
    14521 cagttatgcg ataaactggc tattttgaag aaaggtgaat tgatttatca gggggcagtt
    14581 agtgaattat tgaatggctc acctgatgaa acgctagaaa agatttattt gaaaatggct
    14641 ggaaggcaag cgacaggaga tggaccgtat gtataagcga caattattag aattaattcg
    14701 agttaatctt ttatatgtta atccacagct cacgaataaa gcaagagaga aaggaaaaaa
    14761 gggaaagcga ttaactatct atcttttgtc tcaaaatctt ttttcagggc tgattttttt
    14821 actgatttac ggatttgcta tgtttgctat gaactttgtt aaattgccag gtttctttac
    14881 ttattacgtg gcactttttg gtatgttagg tttaactcaa agtatatcag tcatttataa
    14941 tatctttttt gaaggaaatg acctgcaagt atttttgcct ctgccttttg gacaagaaca
    15001 ggttttttca gcaaagattt tggtagttgc tttgaccatc attccttttg tttttccgct
    15061 tctagtcatg tttattttaa ccggatggcg agctgatcta gggattttgc ttactatcat
    15121 tatctcaatc cttttgtttt ctctattttt gattattctt ttttgtctgg ctagtttgat
    15181 tgtttttggt ttggctcgaa cagcagtgtt taaaaaacat agaaaaacag tttctagtat
    15241 cttgcttggc agctcgatgc tgatcgcatt tgctggaatc atgtggatga atcatcaaac
    15301 ggggactatt gaaaatgagt taacagatcg gcatccgatt tcgatattat tgccattcta
    15361 tgatgctgcc agtcagccac tttctcaaca aggtttattg ggttggggaa tactagctat
    15421 cacggctctg ggatttttcc ttgctgtaaa acagctgata gtaccaaagc ttacagagca
    15481 actgactgat tcaaacagtg agttcaaaat aaaaagaacc cataaaaaga accaaaatgt
    15541 gcatcagctt ttatttagtt acaatgctca attagtaaaa gaacctaatt tgattatgca
    15601 agttctttct tcttccttgt taactccaat catttttatt cttgcttttg ccctaggagg
    15661 cgaaataaaa ttaactgatt tagataataa gttaatcggt gttgttttcc tggtagggat
    15721 cgcattggct ttcttaacag ttaatcagac ttcttttatc agcaatctta tttctttgga
    15781 tcaagaaaat tttctgttta tccgttcttt gccaatctct atgaaccaat atttaaaaga
    15841 aaagttcaga ttcggatgga tattgcagag ttttttaact ggtgggatcg cattattaag
    15901 cggactgtct tttcagctgc ctttgttttt tatccctagc ttgctatttg gttctttatt
    15961 cggctgctac ttattgtcac tacgttattt cgctagagat tatcggttgc tgctgctgaa
    16021 ctggaccaat attaaccaat tatttaacag aggttcagga agtttgggtt tagtcgcaac
    16081 tatgatgggt tcattgatca tcagtattat tcttatcgtc ttatacggaa tgttggcttt
    16141 gttttatcca ttttggttag tgaatattcc tgtgatcggt ttagttctaa tactttccgc
    16201 tctatggatc gtttattatc aacgaacgtt ttggaaaaaa tttgaataaa gaagaggggc
    16261 cgtgacaaaa atcatgtcgc agcccctttt ttacagatag agggtgttca aaagtcagtt
    16321 tctcagtatt aagtcaattt tatgtaaaaa tgtgagaagc tatttttaca aaactttttt
    16381 taatactcat aattacttct tattgctcct gcggttactt gtcgcaatgt ttgtgagata
    16441 ttttgtcatc gctcaaggct gaacgtctct ttcacaacct tttctttgtt acacttcgtt
    16501 tatttaaaaa gccgtttttt tcgtttcatc atgattgcgt cacgctttac tttgaaatta
    16561 cggatgttat ggttaaagac ttctgataat acaagaccta aagcgattgc tccagcaatc
    16621 ataattgctt ggaccgcata atgagccgct tctcgatagg attcagtaac gagatttcga
    16681 accgcttgat aagctaaacc accagggact agaggaacca tcccgggaat attgaaaatc
    16741 gttacgggca ttttcaaacg tttagagaaa agatcgctga tgaatgctac tcctaaagaa
    16801 ccaagcaatg aaccaaaagc agcagatccg cctatttgaa cacagatcca gtagatcatc
    16861 cagccaaaag caccagaaag accacaacaa atcaatgaac gcctaggtac atttgtaata
    16921 acggcaaatg ccgcagtcgc taaaaaacta aaagaaaact gaataaatat cgtccccata
    16981 cttgtgttcc tttcctaata aaaaagctgg aaaataaaag caatcgcaaa accgatcatc
    17041 gtagcagtca tcatcgcttc tgttccgcga gaaactcctg agacataatg tccagctagc
    17101 aaatcacgga tagcgttcgt aatttgaact ccaggaacta aaggcatcac acatccgata
    17161 atgattagat cctgattgct tcctaagcca aaacgtacgc tgtagatagc ggctatgcca
    17221 atcaataaag aagataaaaa ttccgataaa aatttgattc taaaaaattt caaactatat
    17281 agataagctg cgtatcccaa tcctccaatg actaaagtca caggtaaatc ggtcatcttt
    17341 ccgccaaata acaccataat tgttccactg atcactgccg cgctaacgat ttgcaaccag
    17401 acaggaaaga aagaacggtc tcggtcgatt gcttgcagtt cgcagtaaag ttcttctaat
    17461 gttagctctg aagcaacata tttacgtgaa agatcattta cttttactac tttttctaaa
    17521 ttaatcgaac ggttggtaat ttgttccatt cggatggtcg aggtacgatc aagaccaata
    17581 aagagaccgg tttgagtgac atagctgacc aaacggaaat ttccagaagc taaagcaatt
    17641 cgactcatcg tatcttctac ccgatacatc tccgcattgc tttccatcat gattttccca
    17701 gcgagtaaac aagtgtccag caacagatct aattcttttc ccgtcatagc atcttctcca
    17761 ataattgatt gattagtttt attttactac aaaacatacc agaagcgtaa agaataaaaa
    17821 aacttgagga aggaataaaa gaggcgtata ctgtttttgt attgcgggtg tagtttagtg
    17881 gtaaaatccc agcttcccaa gctgatgtcg cgggttcgat tcccgtcacc cgcttaaaat
    17941 aggttgtgaa aaagctgctc tgcaacaagt aataagaaca gccaagcaaa aacaacttct
    18001 tatgttttta acttggatgg acttattacc gcagatgcaa gcttttgcgc accgtttacc
    18061 aagcggttgt gaaatcagtg tattgtcttg agtatgaaga aaatttttga ataataataa
    18121 ataccttaca gatagagtgg tgactgatta gagacgagga atccgtctct tttttgtatt
    18181 tttaagccat cttaaaacag gttttatttt ttgctgtttc tatttctttc tagtatttat
    18241 ttatagggga acatatgtta agatttaggg agctgttagt aaaggagtaa agaaatgagc
    18301 aaaaaaacaa ccaaggcgat cgtggggatc gtcgctgctg tgattgcagg ggcattcggt
    18361 gtttccacca cacaaaaaga ttcagatatc tttcagcaga ttacaactat ttttgattcc
    18421 acaccacaag cttctcaagt agcggatgtg ccagaatatg atggaaaaca tcaagaaata
    18481 gaaatcaatg ggaatcagcc ggaatttaca aaagaggatc ttagcctttc caaaggaaca
    18541 tgggaaagct attcaaatat tgataaattg aacagagtag gacaagccaa tgctatgctt
    18601 ggaaaagatt tgttccctac tgaaaagaga gagcctttgt atattgaccc gacaggttgg
    18661 aaacaaaaga aattaagtga cgggcaatgg ttatataatc gcagccattt aattggtttc
    18721 cagctgactg gtcaaaataa caacattaaa aacttgatga cagggacacg ttcgttgaat
    18781 gctccataca tgcttgctca cgaaaatgat attgcggcat atatcaaaga aacaaatcat
    18841 catgtacgct accgtgtcac tcctcatttt gaggggaatg aactggtagc caggggagtg
    18901 caattggaag cagaaagcat tgaagacaaa aaaattgaat tcaatgtctt tatctacaat
    18961 gttcaagatg gctacacgat caattatgag acaggacaag caacaaaaac aaaaaattag
    19021 tagcagctat tcgaattttt tatgaatatt atgaaaaaat tgtgtaaaaa catacattca
    19081 aagaaatata gcgtatactt tatgaaaaga gtacttttga taaagctaca gaaaaaagga
    19141 aaaaaacata caaatagtgt ttcttaattt attcggaagt ttggaatatt tgtttgtagt
    19201 tttccctttc ttcgagtaaa ataaggttgt agaaggtaag ctggaattac cttgcgggaa
    19261 agaggtgtag aatcatgggt tttacagatg aaaccgtacg ttttggtttt gacgatagtc
    19321 gcaaaaaaga agtgagcgaa acattagcga ttgtttatcg ggctttggaa gaaaaaggct
    19381 acaatccaat caatcagatt gtcggctact tgctttctgg agacccgtcc tacattcctc
    19441 gttatcgtga tgctcgaaat ttgattcgcc gtcacgaacg cgatgagatc atggaagtaa
    19501 ttgtcaagga ctacctctca aatcatggag tgaatttatg agagtaatgg gcttggatgt
    19561 cggttctaag acagtaggtg ttgctatcag tgaccccttt ggctggacag ctcaaggagt
    19621 agagatcatt cggatcaatg aagcagaaga agagtttggc tttgatcggt tgaaggaatt
    19681 agtagcacaa tatgaagtag accggtttgt agttggatta cctaaaaata tgaacaattc
    19741 catcggacca agggcagaag cttccatgtc atacggagaa aagatcaaag aactattctc
    19801 cttgccagtt gattatcaag atgaacgatt aaccactgtt caggcagaac gtatgctagt
    19861 agagcaggca aatacttcaa gagccaaacg aaaaaaagtc atcgataaat tagcagctgt
    19921 gatgatctta caaaattatt tagacggctc aggtaagcaa ttattttaat aataaaagaa
    19981 aagaggtaat cttatgacag aacataacca ttcccacgac catgaaggac acgaacacat
    20041 cacattggtg gatgaccaag gaaatgaaac attatatgaa atcctactaa cagttgacgg
    20101 acaagaagaa tttggacgta actatgttct tttatatcct gctggtgttt cagaagatga
    20161 agatgtagaa ttacaagctt atgcatacat cgaaaacgaa gatggaacag aaggcgagtt
    20221 agaacaaatc gaaacagacg cagaatggga catgattgaa gaagtattca atacattcat
    20281 ggcggaagaa gaagaataga ctataaagct aggagaaacc ttgatatgat agggtttctc
    20341 ctttttttat tttttactgt atgccatatt gtatgccatc tagtcaatat ttaagtgttg
    20401 tactaatttt tcaacagtct tttctttcgt ttcttttgcc aagtgaccgt aacaatcgat
    20461 cgtctgacta atttttgtat gccgtaatct agtttgaact tctaccatag tagctccagc
    20521 gtccaagagc attgaagcat gagtgtgtct tgctacatgc acaccgagaa atggaaccat
    20581 agctttttca caaatagtaa tcaatcttgc ataaatagcg tggtgtgtga ttagttcttt
    20641 atagatacca caaaaaatca tagtcggatc agttacacca agtgtcagca tatatttccg
    20701 ctgattaatc tgccattttt ttaatataga aattgttctt tcatctagtg gaagaacccc
    20761 agcactacaa tcagttttag gatcactgat tttatagcca tttttggatt ggcttagtgt
    20821 tttattgatg gtgaccgtat tttcttgaaa atcaatgtct gaccaattca atgcaagggc
    20881 ttcactgatc ctaaccccgc tataaaagag aaagcgtagt aacgttttat caaagtcgtt
    20941 tcgataagtt tcaggtttgc tgtttaggta atcaaacaag gtgatcagtt gttctttact
    21001 gtaaactttg accttttctt tatgcttagt ggtggttctt ttaggcatta atacgttgtc
    21061 tagtggatta gtctcaataa tcccaagatt gatagcgtag ctaaaaatac gcttagaagt
    21121 tgaatgaagt accttgtatt ggtcagtagt ttcagcccag ctatttacaa tttgttgggc
    21181 tttttttacc gtgacttcat tcacacgaag atttccatat tttggaagaa tccactttct
    21241 aaacttgatt tcagttttca tgaaggtggc ttctcggaca gtggttttat atgtttctag
    21301 ccacaagtca taaacttctt taaacgtttc gttgttcgct tttttaatac cgtttttatc
    21361 gtagtccact tgcaagcgtg acaaggcaag ctgggcttct ttttttgttt tgtaacctcg
    21421 tctacgaacc ttatcaggtt tgccagtcat tttgttcatt ccaagatagg cttcatagag
    21481 ccaaaattta ccttttttag tttcatattg cttgaatgtt gccataatag ttttccctcc
    21541 atagtgtagc aagccgtggg ggcggttgga ggaggcagtt tattattatt ttgaatagtg
    21601 tttatttagc tcagtaacaa gagctgtgaa ctcttttaga agatcatttt cataaaattc
    21661 ttgatactct tttttctcat ctgtattttt ttctgaatca ttaattaatg agtcatatcg
    21721 taaaagcgta tttagaagag ctgcgagata agcaattggg gtactcccgt caggaaattc
    21781 aattcgagat cctgttttaa gatattttat tgcgtttgtt aaataaatct tctgtgagac
    21841 agtcaggtta tcttcttcga tgttattgat agcattagaa agattagaat caattataga
    21901 gttatttttt tcaatagttt ttttcaattg cttttcaagc acatttcgct taatttcggg
    21961 catcatatgc atatctctta atgtataaga tccgtttaat agataggtag tggatacatc
    22021 taataccttt gaaagttgtt taagcctggt gttattggga agattatagc cattttccca
    22081 attactaacg gcacctttac tggccggtgg ggataattta cctccaagtt gttccattga
    22141 gagtcccttt tcaattctag ccagtctaat tctttgtcct gtctccactg cttcggggta
    22201 tttttttcta gccacatttt caccaccatt catatatttt ctctactata tcacgattta
    22261 aatatgtgtg aaacataaaa gttcaaaaaa gtatgttaaa attcttgcaa atgaaaaggt
    22321 atgatggtat attgagttta taagaggtat agaaatgtat tcaagagcct cttgcatttt
    22381 tttaaaatct ttgtatagaa atgtatgcta acgagggggg ataaatatgg aattaaaaat
    22441 tgacaaactc acgcttgatg atccagcttt taaaattatt gacgatcgag ttgttcaaca
    22501 agtagagcaa actcaaggaa cgttactttc attattagaa aaaaagtacc agtttccaaa
    22561 atatatgaac aaggcacaag ctgctaagta tatgaacgtt tcatacaata ctatgatgca
    22621 gaaatatgtt cctaacggtt taaagttgat tatagttgat ggagtcgtaa gaatttcaca
    22681 agatgaatgt gatcgcttta tggaagaata ccaaaaataa acgcaagccg tgggggcaaa
    22741 gggattaata ggaggaaata ataatggaat cgacacaaaa taaagcaatc gaaaaagtat
    22801 tatcaacaat tcttacagaa gacacagcgg cagaattagc aaacttggaa ggcaaagcgc
    22861 tagaagaaac gtttgaatgt ttgtacgaac aaatggacta tcaaaaattg ctaccacaag
    22921 aaccaacggt aagcggagta ttgcgtggct tgaacgattt agtacaagca gagtttaaag
    22981 aacgactttc tattgaagag tatcaagaaa ttttgtatca gcaagttgag ttgttagcga
    23041 gcttgctagg gcttgaactg gaggacgttg aatgaaaact gaaatttgga acggacacat
    23101 tataagattt gtcgatatta atgatgaatg gtgggcagtg gcgaaagatg ttgcggaagc
    23161 gttaggtctt aaacaggtta ctagagccat tcactctttg cctaaagatg gggttactac
    23221 tagtaaggtc atcgattcat tgggcagaac acaagatgta aacatcatca acgaaaaaaa
    23281 atatttaccg catggcattc aaaagccgta aaaaagaagc cgaggcattt caagactgga
    23341 ttttcgacat catcaaagaa ctacgccagt ccacaggact tgaaggcttt caagtattcc
    23401 gcatgctaga caaagaacac caaaaagaag caatgactaa attaagccac gctattactg
    23461 aaccaaaacc agtagactac attaaagcga atgtgatagc caataaagct gtatcaacta
    23521 tttatggtca ttccaaaatg gtaaaaaaga aagatatgac tcctgaaatg ttggttgatc
    23581 gtgaacctat tttagatgag acagtagaat taatgactgt taaagaaaaa tatgggttgc
    23641 agttttcagt gagtgagaaa atttataatc gttctgctga attgcagaca acttaggagc
    23701 attaacatga tcgagaacaa agaaaaagcc ttatccataa cagtcttggc ggacaggata
    23761 aaggcaactt aattgggata aaacccgata ttctttgttc cgattatacc atattaaaaa
    23821 atcggaggga accattatga aacgaaactt aattatagga agcattttaa tcggcggaat
    23881 tttcggctta ttaccattaa ttgcaaaaat agctatcttt gcaggattag cgttatatct
    23941 cttcactgct tatgacgagt acgactatca attaagaact ggagggcata agtagatgta
    24001 caaaacaaaa ctattaaacc aactcgatag ccttgaatta gaggaaatta atcaagggat
    24061 cgctgaacta gaaaataata tcgggaaaac ctactttgga aattcattca atgaaaaact
    24121 aacagttttg tatgtactca aaaaacatgc agaacacaaa ataatttgtc gagaaatcaa
    24181 cgagctaaaa aatcagattt taaccgcatg gttaaacatt actgatatgc aagaagcaag
    24241 ggtgaaaaca tttaatacat gggtaaagta tcagaatcaa ttaaaaggtg ctgaatttgt
    24301 tcgggatggg ttgaaatacg aacttgagca gttgaagctc atggaggtgt ctgaatgatt
    24361 tataccagta ttttgaaaaa ggaggagagg tttattggca aataattatc aagcagcaac
    24421 ggaattacaa aagtcgggat ggtcaattta tcccttatct cctggaacta aaattccaat
    24481 aaaaggctct agtggttgga atgatgcaac taacgattct aatcaaatta atgaatggtg
    24541 gacggaagat gcaactagaa atattgggtt aatgcttgaa gatcatcaaa tggtggtagt
    24601 tgacctagat caacattctg aaacacgaaa cggcgttgag aattataaaa aacttgcttc
    24661 aatgtatgat ccatttcctt ctacttatac ggaaatcaca ccaggtaaag gaatccattt
    24721 cttctttaaa attcctgatg gtgtaattat ccaacaagaa acaggtgcat ttgctgatat
    24781 cttcgggcgt gatgataaag gtaaaagttt aagcgggatt gatattatca ctaaaggagt
    24841 tccaatagcg ccaactatga ttatttcaag aagtgttggg agagcatatg aacctattga
    24901 aaacgtatct ctaaataata ttgcaccagc tcccgattgg ctagtgaacc tactgctaaa
    24961 aaaaccgcaa gaaataaaga ctcaataccg acccgtagca aaaacaggga cagcaaaaaa
    25021 attagatatg attgttcaag gcgctggcga aggtagtaga aacgatcatt taactaagtt
    25081 atgcggttgg ttactatggc acggtgtaga taatcaaact ttagcagaat tgatttatac
    25141 agcaaatgcg tacaatgaac caccgctaaa agataaagaa gtgaatcaaa ttattcgatc
    25201 gatgattaaa aaagatttga gggggaaagc tcatggctca taagcataaa ttagatagta
    25261 ttttagactt tcccgaagcc agcgagcgtg aagacaatat cattgaattg aaaacctgga
    25321 tgtcacgatt aagatgcaat aaagacgatc agattaaaag taacagtgtt gtgaatgcag
    25381 aattgatttt aaccaatgat agcaatttag ctggaacaat tgcttataac gaatttagcg
    25441 gatacattca cctattaaaa gattctcctt ggatcaatcg taccgctgga gaatgggaag
    25501 acagttttga ggatgcctta acggcatata ttgaagaaaa ctacaatgta gtttttgatg
    25561 acaacaaaat acacaaagca gtagtaaatg tggctagaaa gaatgttttc aacccagtaa
    25621 aagaacgtat cgaaaaagtt aaatgggatc aacagcctag actggaaaca ctgtttattg
    25681 accttttagg ggtagacgac aacctttaca cacgagaagt aacaaaacgt tggatagtcg
    25741 gatcagtcgc acgtatctat aaaccaggaa taaagtttga gatcgttcca gttttggatg
    25801 gaccgcaagg gattggtaag agtaccgttc cagcattact ttacacggat gattttttca
    25861 ccgattcatt agattcgcta ggtgaaaaga aagatgatta tatgcagctt caaggcaatg
    25921 taattattga acttggagaa ttatcttcaa tgaacaaaac taaaattgaa cagataaaaa
    25981 acttcatcag tgccaagatc gataaaatta gacctcctta tggtcggaat gtcatagctt
    26041 gggcaaggca atgtgtattt attggaacgt cgaatgatgg gcagtatttg aaagatgata
    26101 caggaaaccg ccgtttttat cctttgcctt gtaagaataa gccaaagatg aatccgttta
    26161 aaacaaagga tgaatatttt cttcaagtgt tagctgaagc aaaggtgctt tttgataaag
    26221 gacaacgaat ctattttagt cccgatgaag ataaagaagt cttagaaatt gcaaaagact
    26281 atcaagagga tgccaagatg gagaatccaa ttaaagaagc aatcactaaa tatttggaaa
    26341 tggaaatccc gtataaatgg gaagaagctc cagcgtgggc gagacgttct tattatcaga
    26401 actatcctga cagtaaatgt aatgagaaaa atttacagca tttctataat ggagctacta
    26461 tgcaacaaga gttttattta gttgatagcg tactcaccgc tgatattttg gaagctgttt
    26521 ttgataaaca agcaaaggat ttattgaatg gtcgatcaga tgcagaaact aaaaagattg
    26581 ccttgattat tagcagtatt cctggttggg aacggaagca actgccaaaa agaaataaac
    26641 gacgaggatt ttacaataag tcaaatgcct atgaaaataa aaatcgagct ggaaagtaca
    26701 aaaagtaacg ggtagtaacg ggtcagtaac acctagcccg ttactagttt acccttactg
    26761 ccccaaggga tacagcaatt tggtaacggg gtaacacctt atttccttaa aacttttgaa
    26821 atagttagct aatatgctgg ggaataatgt tactggattt tacccgttac ctgttaccaa
    26881 aacgctagaa cccttgtccc cgttgggatt gagtggtaac gggtacctgt tacgtaggtg
    26941 ttaccacgtg ttacgaatca aggaggaaag taaatgctca atattatttc aacaaacaaa
    27001 gcaccgaact ttcaatatac agatgaaatg gatcggtttc taatgaacac gctggcattt
    27061 agtgtgggat tggtaacaga agattattcc acatttgatc cagaagtatt aaagataatg
    27121 gaagaagaac ctgattggct gcaggagtca gtagcgtggt gtcaatcgct agtagtggga
    27181 tcattggtag atagtggcaa ctatgatgat acaggtgagc taatggatga atttaattgt
    27241 ctgcttaacc tatatgatcg agcgagacaa cgagagctta catcgaatga ggataatttg
    27301 tttttaaaca tccatgataa gttcttggct ttgctactaa cagatgatga attgataact
    27361 aatttattgg aggtagaata attatgcata tggaaagtat tagatacttc gatggtgaga
    27421 aacacattga gtattcaaac actcaagaag atgtagactt tattagtttt gaggaagata
    27481 aaatcactaa agttaatttt aaagacggaa cgtgcttgaa gatagtttcg ccttatattg
    27541 aatacaaatc aaaatgggaa caagatgatt ctgacgaacg agatcgcttt ggttggtaat
    27601 ctatggcagt taaaaaacaa tgtaatcatg ctggatgtaa aacattgatt gattataggc
    27661 agaagtattg tgggaagcat aaagcaaagc aaacggcaat taagcgtgaa gaaagaaagc
    27721 aaagcgaagg taaatatttt caattctatc aaagtagaac atggcgaaag gcttcatact
    27781 tgtatcggtt aaatcatcca gtatgtgagg attgcctaga agaaggcttg ataaggaaag
    27841 ctgatgttgt agatcacaaa attgaactaa aagatgattg gaatagacgg ttagatgaaa
    27901 ctaattttcg ttctttatgc catgcacacc ataattccaa gacagccaat gaaagacaaa
    27961 gacgagaaaa gagtaccctt tgagttaagg gggcatagtg tgaaacttac tgacaatcga
    28021 tgcctactca ttttggtaca aataaccgtt gtgaaaaggc ataagggtaa ttacaaataa
    28081 cgattacact tgtaatcaca agtgaaaaag tatataatga aagtaggaga tttattgaaa
    28141 aatacaattg ttattactga taaaacaggg aaacagcgca ccattgagtt acctcatttt
    28201 ggtcaagtga ccattcaaat gcagaacgga aaaatcattt atatagataa attagataaa
    28261 gaaaaattct gatcgaaaaa cgaaggaatg tgagcttaat tgcttacgtt cctttttctt
    28321 ttgtctgaaa ggaggacaac atgggacacc ctaaaatatt acacgataca aaaggcaata
    28381 tatcaagtga agaaaaagca atccgtgtgg acgctagaga agaattgttc aagcagcaac
    28441 cattaataaa tatcacgccc cctgactgga tggcagcgag cgctagaagt gaatggaatc
    28501 gtatcgtacc aacattaaaa aaagattatc cattgagtga agcagattat ggttcattgg
    28561 tagcctattg tttagccttt gctcgaatga aaacagccga agccgagata agaaaatcag
    28621 gtacgtttat cacatgcgaa aacggagtaa agaaagccaa tccagcggtt cgggttcaat
    28681 cacaagctat gagtgatttg aaaaaacaag ccacctcact aggtatgacc ttagaatcac
    28741 gatcaaaact agctttgaac aaggttaaaa atgatgaacc cgaagacccg ttcaaagagt
    28801 tgatgaattc atgaatgatt acattgagaa agtcctatca ggtaagctga ttgcacccaa
    28861 gaaaattatc caagcgtgtg agcgtcatat aagcgatttg gagcgttcta agtcagatag
    28921 cttcccttat gtgttcgatg aagaacaagc caccaaagcg attaaattca ttgagttgct
    28981 accatctacg gacggtaaag caatcaagat gttaggattt caaaaattca ttctaggcag
    29041 tctttatggc tggcgtacta aagaagggaa ttacaggcga ttcaatcgag cgtttaccag
    29101 tatgagccgt aagaatggga aaacgtatat cgcaagtggc atggctgcca atgcgttgat
    29161 tatggaacaa gaaccagcag aagcaaggca agtattgttt gtaagtaacg ctttgaaaca
    29221 agctaaattg ggctatgata tgctgtctaa ttcacttaga aacgtggtca agtctagtaa
    29281 gtttttaaga ccacaactga aaattatgaa ctctaagatt cagcacttgc cctctaattc
    29341 gttcgctatg gcactggcta gtgaaaccag cacgctagat gggtttgcac caacaaccgc
    29401 aattcttgac gagtggcacg aagcaaaaac tcgtaaaacg tacaacgtca ttaagtcagg
    29461 aatgacccaa caaaagaacg gcttattgtg tgttattagt accgctgggc ttgatttaaa
    29521 cgttcctatg tacgaggaat acttattgtt agatcgtgtg ctaaaaggcg aagaacaagc
    29581 cgacaggtac tttatagcga tatgggaatt ggacgatccc gaagaaattc acgatcaaga
    29641 gaaatggatc aaagccaatc cgatttttga aagtgaagaa atcaaaaaag taatgattcc
    29701 aaccattcaa gatgatgtga accttgcttt gaaacaaaat aaccttaatt ctgtattggt
    29761 gaaaaacttc aatttatgga gacaagcgag tgaggacagc tatatgattg ctgaagactg
    29821 gcaggcaacc gaagtagaac cacaagatat tacaggcaag cccgtttata tcggagtgga
    29881 tttatctaaa acagatgatt taactagcgt ttcatggatc gtaccgctag ataacggcga
    29941 actttattgt gattcacata gctttgtagc caccaaatat gggcttcaag acaaagaaaa
    30001 gcgcgacggt ttgccttatc gagaactaga aaaagctggt gagtgttcca ttactcaatt
    30061 agaaagtgga atcgtggact atgaccaagt atttcagttt attcaagatt tgattcaaga
    30121 aaatgatttg gaatgtatgg ggatttgtta tgacccgtat aacgctaatt cgcttatcag
    30181 taaagctgaa aaagccaact acccaatgtt agaagtaaga caaggaacga ttactctaaa
    30241 cgttccgacc cgaactttta gagaacaagt ttatgaaggc aacgttattc acaataaaaa
    30301 tacgattctc acccatgcag tgaacaacgc tattttaaaa acggataaca acggcattca
    30361 gattaataaa tcaaagaaca gtaacaaaat tgatccaata gctgcattaa tcaatgccta
    30421 tgtgtttgca atggattact tcaccacaac ggaaggagcg aaagcagaca atgaatttta
    30481 tacaagtgaa gaattttctt tctaattaca ttcataccgt tcttttactt cttggattgg
    30541 tgtgtgtgtt ggttgctatc accttactaa caaatgtcta ctatggcttg ttagcgctgg
    30601 gcatagtgct tattggggta gcggtcatgc taaatacaga acagaaggga ggttaaaaga
    30661 tggcattttt taaagcgaga caaaatacaa cgggagatcc tttcctacaa catgtggtat
    30721 caatccaatc ggatgattac accaccagct ttacaagcgt tcgtgcatta agaaatagtg
    30781 atgtgtttgc agccgttcga attattgcta gtgatattgc ttcaagtccg attcaattgg
    30841 tcaaaaacaa tatgccacaa gctgataatg aactggtgaa gttactaaac gagaaaccta
    30901 attcagaaat ggatggttgg catttaaagt ttgctttggc ggtcaatatg ttgctaaacg
    30961 gtaatagctt tgcagagatt aaacgcaacg gtgaaaaggt agaagaaatt cacttattac
    31021 ctaactcaag tgtaacggtt actcaattag ataatggtac gttgtcttat cagattggtg
    31081 ataaaaaaag acgtgtgaag tctagcgata ttttgcactt caaatatttc acccaagatg
    31141 gtttaacagg attaccacca ctttatgcgt tacgtgatga actgaaaatt caacaagctg
    31201 gcaaccgcac attgcacaat ttctttactc gtggtgtcag tgggtcaggt attttgaagg
    31261 ttcataagtc agatttagac ggatcagcaa agaacgcaat tcgtgaaaag tttgaagaag
    31321 ccaacggttc aagtagtgga gataatgctc ttagaacgat cattcttgat gaaacaatgg
    31381 actacaaaac attagaaata aacaccgatg ttttgaagct tatcaattcc aatgattgga
    31441 acacaaaaca aattgccaag gcgtttggtg taccgattga gcgtttaggc gttgaaaatg
    31501 aacattctag caccgttcaa agtaatctcc agtatatcca aagtacgctg attcattact
    31561 ttaatgtgtt tgtgagtgaa ttagatacga aacttgaaac caatatccgc tttaattccg
    31621 atcagttact agagactgat cctgaaacaa aagtaaaaaa tgtattggat caggtcaaag
    31681 ggtcacttct cacgattaat gaggggcggt cgaaaatggg gctacccccc atggatggtg
    31741 gcgatcgttt actagcaagt ttgaacttta cgtatttaga tacgttggag aaatatcaat
    31801 taaaagaaca ggaaggagtt acaccagttg aataatgaag aagaaaagga aaaacggctg
    31861 acagaagaag ctgagctaac agccgattct cccaaagtag agaaagaaaa tgaaaaacaa
    31921 ccaacagacg gcaaaactat ttcaggctat gcgttgaaat tcgggcaacc gtcaaaagat
    31981 ttaggcggct ttgtagaagt gattacaccc gaagcattaa aagaggtgga tttatcaaat
    32041 gtgttcttat tatatggaca cgattattca aagccactag ctagtgtcaa agcaggtacg
    32101 ttgaaattga atgtcgatga tacaggatta cattttgaag cagagctaac cgacacgacc
    32161 tattcaaatg atgtgtatga gaatatctca aaaggggttg ttgattctat gtcctttggg
    32221 ttcgtgcttg gattagattc attcgacaaa aaagaagatg gcacaattga acgatcaata
    32281 gataaaatca aagcacttaa tgaaattagt gtcgtgaccg ttcccgctta tgattcatca
    32341 aatgtccaag taaataagcg ttcttacgaa tcgtttatga gtaacaacca agcaaaacaa
    32401 acaaacaata gcttagaatc cacttctaaa gcacaaaagg agagtaataa catggaaaaa
    32461 actttaatcg ataacgaaaa aacagaattg cgtgggtatg aagaatatat ccgttcacaa
    32521 ggcgaagtgc gtgatggagt cactactgta aatgcagcgg cagttgttcc cgaagaagta
    32581 atcggtgaag tctttgattt gaaacgttca aattataact tagctcaata tgcaacagta
    32641 aaaacagtat caaatggaca aggtaaatat ccagtagcaa ctaaccaaca agcagtgtta
    32701 gcaacaaaag ctgaacttgc tgaaattggt gatattgacg ctgaaatgtt tacttcagtt
    32761 gattataaag tagaaactcg tgctggtaag attgccttat caaatgaggt tgtggaagat
    32821 tcagcagtga atattgtaca agaggtcaaa gatcagttag cgaaattggt agaaaacact
    32881 gacaataagc atatcatgga tttattaaaa acattcacta agaaaacggc tgctacgttg
    32941 gacgatttga aacaactata caatgtggca ttagacccag cattaaataa aatggtaatt
    33001 ctaaaccaaa gcggatatta ccacctagat acattgaaag attcagatgg acgttacatt
    33061 ttacaacccg atgtgacagc acctagtggt aaatcattat tcggtatgcc agtagtattg
    33121 attgcagata cattgtttgc caatcctaaa gctggaacat tccctatgat tatgggggat
    33181 attgcacaat ctatctttgt tgctcgtaga aatcaagtaa cgactcaatg ggaaaaattc
    33241 gattactact cacaaggact tgcagtgatc gttcgcaacg attataagaa aattgatcaa
    33301 aatgcttcag tgtatattga gtttacgcca gttgtaacac caaaagtata gaaaaatatt
    33361 gggcggcggt ttatcccact gccttttttt cattaggaga gataaaatgg taattttgga
    33421 tagcataaaa aaaagtatgc ggatcgatca cactattgat gatgacttta ttcaacaatt
    33481 gattgataca gcaggcgaat atataaaaag tgctattgat agtagcgcaa cggataaaga
    33541 tatggataat tatcagcaat ttgatttggc ggtgtcatta cttactcaac attggtattt
    33601 gaaccgtcaa gaagccagca gcgaacggat accagtaacg gtacaagcat tagtacaaca
    33661 aatgagaggt gcttattatg ccaatcatta agaatgtaaa tgaattgact gagagagtca
    33721 agtttaaaaa gacaaaacgg gtaaaagatg aagatgggca gatggtggat agtgaagaaa
    33781 ctgtatttga atgttgggct aatgtgcgtt cacaaatgct aaaagacgtg cttgctagtg
    33841 taggtaccat tcttgaagga acgttgacgt ttattattcg atacgatcaa gattatgaac
    33901 taacaaacga tatgaaagta gcttggaaaa acaagagtta taaaattatt tcaatcaatg
    33961 aaggaacggc gtttaaagat tatacaacga tcatagctaa acagatttct tagaatgatt
    34021 acacttgtaa acgttgtggg tatttagtat aattaaggta gtaaatgaag ggttagctgc
    34081 ctgaattttg caagacttag ctagtcgaaa tctattgttg gactgaaaat tgtaggtgtg
    34141 gttgcaaaca tgctggacta agaaagatga tagatgatga acgttcaatt ttaatacagg
    34201 catgctagga ctgattggat ggttttggca agctgattgt gtagaaatca aagaacacgt
    34261 ctgttgtgga cgtgtttttt ttgttataat tcaaaagagg tgatttaatg gacaaagcag
    34321 agtttacaaa tttttattgg aagtattatc tacatttaga aaatgagttt attaatacca
    34381 cagattttgt gatgctcgat gagaataatt tcaaaacatt ctctattgaa tatcaaaaac
    34441 tactactagc gattggttcg gagtgtgaga ttattttcaa agaactttgc ggatttgata
    34501 gcagtagcaa taaaagcata actgatttta aagctgttat taaatctagc gaattaatgt
    34561 gtttggataa tggtgttagg gttatgaatt ctttaacatt gacttcacta aaaccttttg
    34621 gagatgggtg gcctgaagat acaccagatt ggtggaaaat atataacaga gtaaaacatg
    34681 gtcgttcttc aaattataag gaagcaaatt tggaaaatgt gttgtatgct ttagcaagtc
    34741 tatatttatt agaggaatat ttgcataaga aagtaatact agaaggtgaa gtagacttta
    34801 taagtccaga gtcaaatcta tttacgttat cttgggaaag aaaacattcg tcgatgcaaa
    34861 aagtgacgtt tgaaaaagtg gattcaaatt atgtagctcc actcattgat tttaaagaac
    34921 aagaagaaat ttaaaaaccc ccacggctta ttgaagtttt tgtatgccat attgtatgcc
    34981 atttggttta aatcgcttga aaaatagtga tagataactc taattcattt agtcataaac
    35041 gctgttttga taacctttat agttctttga tatgggtttc tcattcatgg cggaagaaga
    35101 agaatagaac aaaagaatga attaataaga tataaaaaaa gggattgtga caaacgtctt
    35161 gtcacaatcc ctttttccaa ataaacgatg ttcaatcagc tgcccctcgg cattcgctcg
    35221 aaaaacggcg aaatatgaag aactattttg ccatttgttt ctctaataat caggtcaaaa
    35281 cgttgacttc acagtctctt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt
    35341 cacaatattc aacaaacatg agaagctgtt ttttgaaatt gttccttgtg actcggagct
    35401 gaacggcagg ctcacgacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat
    35461 aagttcaacc aaacgaaaat tatgaggagc tatttttgtt tggctgttct tattacttga
    35521 tgcaggacag ctttttcaca acctcttttc gtttagcact aaatttcctt aaaaagtaga
    35581 aaagctttcg aagcctcgtt tgtttctttt actgtataat aaaagaaaag agtaacaatc
    35641 gctaaaagat ataaacaaga gaaaggaaat acaagatgat tggtctgatt tattttgtgg
    35701 tgatcgttct ggcgaacaca gttggcgcta tttccgggat gggtggaggc gtgttgatca
    35761 aaccgattct tgatcttatc ggcgcccatt ctgttgcagg gatttctttt tactcaacag
    35821 ttgcggtatt cacgatgtcc attgtttcga ctgtgcgaca agtatcaagc gggaaatcac
    35881 tgaattggca gattgtcggt tgggtatcta gtggagctgt tgttggagga gtagtcggca
    35941 acattgtatt cgaagttttt ttacagttat ttgaaaacga gaagcatgtg cagctgatcc
    36001 aaattttttt gacggtattg accttggttt ttgcgttctt ctacaccaag catcatcagc
    36061 caaaatttca tttgacgtca tggacttggt accttatatg tgggggggtt ctgggctttt
    36121 tagctagttt tttaggtatt ggtggtggac cagtcaatgt ttctttgttg atgctgatgt
    36181 ttgcattgcc aataaaagaa gcaacactat attctctatc tacgattttc ttttctcaac
    36241 tggcaaaatt ggtaacgatt gctttaacaa gtagctttat gcgctttgat ttatctatgt
    36301 tgttttatgt tatccccgct gctgttattg gaggtttgtg gggagcaaga tttagcaggg
    36361 ttttgtctcc aaaaaaagtg acctttattt ttcaagtaat tgtcctcgtg gttcttttga
    36421 tcaatcttta caatgctttc gtcatcttac aaacattagg agaatctaga ctaacgcaag
    36481 tcaaaatcac tgaaacatat tgttctaaat gaaaaaagga gtatactgtg ataaaaaaat
    36541 tcatttaaaa aaataaaaat tggctggtga tttggatgtt gcgggaaatg cgtttaggag
    36601 atgcagaagc actacgagaa atcaatgcgg aacaattagg atatgatgta ccattaaccg
    36661 ttacggtgag acaaatgaag aaactgttga aggagccaga aaaaaatttt ttcttggtct
    36721 atgaagatcc tacttctaaa aaagcagctg gctatgtaca agcggagtcc tatgaatcga
    36781 ttttcagcga tccgatgttc aatattatgg cacttgctgt gtctaaacat gctgaaaaaa
    36841 gaggaatcgg aaaggccttg atgactggcg tagaagaagc gaaaagacga ggaatatcag
    36901 caataagact taattctgca gaatacagag tagaagcaca ccgattttat gaacgcatcg
    36961 gttatcattc cgataaaatg caaaaaagat ttttgaaaat cctgtaaatt tttatcaaaa
    37021 aatacctggt catcaagccg atacttgatg atcaggtatt ttttttgact aagaaagtaa
    37081 ttgcatcaag aaacctgaga tgatcaaaac gatggcacca ccaagccgat tgcccatttg
    37141 agcaaaagca ataagttcca tccgattagc tgcagataag acagctacat ttcctgtacc
    37201 acccatcgag ttgttgcata agccagcagt aatagttgat tcgattggat ataaaccaaa
    37261 caaacgaccg acaaatcctg aaactactga aatcgcgata acacttgtca aacataagac
    37321 aacaaattgc catgtcaaag cagaagccaa tacatttaga ttcaataacg caataccgat
    37381 accggcaaga acagctgctg tcaagttttt gacaatcaag ttgttgaaca tgatagctgc
    37441 atcttcgtaa tattctggta ataggtttgc gattttacag ataacaacga taatgatcat
    37501 gaatgcgtaa gcatggactt taggaacaaa atagttacag atttgtccta agatgaagaa
    37561 agtcaatgat accatcaaac cgacacctaa ttgtggaatg gaaggtttta catttctagg
    37621 ttttccatcg ccaaggtcgc ctttttccat taatttacca ttaccgttga attttggcat
    37681 tgattgtccc gcacgagcaa ctaacgctgc accaatgata gctaggacat ttgtcattgc
    37741 tgtcgcagga atcaggcggg aaatgataga agaagcgtcc gtacctaaaa catttgcata
    37801 aatcgttgat aaaggtacag ctccggctcc aacaccacca gccattgcgg gcaatgagat
    37861 atataaaacg gaatttgcaa aaccatttcc gataagcata cctacgagtc ctacggcaaa
    37921 aaaggagacg accattgaaa taagtgcaac gggaataaaa cgaacagaag ctttcatcaa
    37981 tagattacgg ttcataccaa ggatgctacc agtaatcaag gcagcaatat aaaagtctaa
    38041 aaagcccata ttgctgacaa agttttctgt ggattttact acatactcag gtaagatccc
    38101 aaatgtagca atcgcggcag aggcaaagat cgcaaaaacg gagccaccac ctaaataact
    38161 tttgacgata gggatttttg ttccgataaa atgaaataaa ttaccgaaaa tgaccaaaac
    38221 agcgattggg ccaatcatat tcgtcggtaa ttttcctaaa gccattgcaa tgatcaggac
    38281 aaggaccatg atcaaataaa ggggcaaact tacgccggta atttttgttg accaaaaccc
    38341 ttcttttttc ttttcctcaa ctgcagttgc ggttgttgtt ttttgtgcca tttttacaga
    38401 tcctttctta ttctgtatcg tatgaaaaaa gccaaagaga agcggcaaaa gagcagtagt
    38461 tggctggctg ctgttttgcc gcagtcgctt ttttcttttt agacgtcata caaagtttct
    38521 gtatgttctt ttactataac aatcttaatc ttctaaagat ttatattcag gaacccattt
    38581 agcagattca acagcttctt tgatatccgt gatttcttca cgattcaatt tttgtgcaac
    38641 aactgatttt gcgacagttt cagcaatcgt ttgagagaat tcagtgattt ttgctactgg
    38701 aggcaagata gctgctcctg gttttgttac atcaacgatt ccgcctaatg ctcgactagc
    38761 ttgagaaatg atttcagcat ttactcgtgt agctgttgaa gcaatcaaac caagccctaa
    38821 acctgggtac atcaacgcat tatttgcttg accgatttgg taagttacac cgttatattc
    38881 cacatcagca gcagggatac ctgttccaac taaagctttt ccgtccgtcc attcaatcaa
    38941 atcttttgct tttgcttctg ccaattttgt tggattagac aaaggcatga tgattggacg
    39001 tggggtatga gccgccattt ctttcacgat ttcttcagtg aatgctccag gttgggtaga
    39061 agtaccgatc atgatcgttg gatggattgc ttttacaact gcttctaagt ttgtcaattc
    39121 atcagcatta gtgaattctg agcgtttacg agcgaatggg atttgaccag gagtcaatcc
    39181 ttttgtatct tcaaacaata aaccctgttt atctacttga tagaaatgtt ggcgtgcttc
    39241 ttcttcaggt acaccttgac ggatcatttc atctaaaagg atgtttgcga tacctacacc
    39301 agcagtacca gcaccaaatg tcaagatcgt ttgatctttc aattgctcac ctgatatatt
    39361 caatccacct aatacaccag ctagtacaac gattcctgtg ccttggatat catcattgaa
    39421 agtagtgatt ttatcttcgt atttttctaa gatcgttgca gcatttcctc gtccaaaatc
    39481 ttcccaatgt aataacaatt ctgggaataa ttcagtagct gattgaacga attgatcaat
    39541 gaattcgtag taagtttcgc cttctacacg agcatgacga ttccctaagt ataatggatt
    39601 attcaataat tcttcgttat tagtaccagc gtcgattgat acaggcaaga cttgtgcagg
    39661 attgatacca gccgcagcag tgtagaccat caatttaccg atagcgatgt caacaccgtt
    39721 cacaccccag tcacccatac ctaagattcc ttctgcatct gtcacaacga tcaaacgaat
    39781 gtcgcgtcca tctgctgcgt ttttcaaact tgctttgata ttttctggtt cgtcgattga
    39841 aagaaatgca gcatcttgtg gtttcaagaa aatttcgttg tattgttcga ttgaatccgc
    39901 aacgacagga tcataaacga caggcatgaa ttcaactaaa tgttcgccca tcaatttata
    39961 aaacaatgtc cggttcgtat tgaatagatt cattaagaag atacgttttt ccaaatcggt
    40021 ctgtttgctt aagtattgac cgtaagcttg gacagattgt tgttccaatg tttggacagt
    40081 acttggaagc ataccagtga ttccgtattt tgcccgttct tcttttgtaa aagctgttcc
    40141 tttatttaga aaaggattat ttaataagtt tagccctgta agcatgaatg attcctccta
    40201 agttttaata caaacgaagt ataggagtca gatgaggata tagtcaaaaa gagcaggtat
    40261 aataaatttt atttatatga gtaatagagt caaaagaaat caaaatagcg gtatttgttc
    40321 tttttatatc aacaatcctt gcttgattac tatgcgtatc tcttatatgc taaagaaaga
    40381 aaagtagaag aataaaggat atgttagata gataaataga atctctgaag aataaatcac
    40441 gtgatcgtga cgataaaata cacttacgtc aggtgataaa aaatacataa gtagaaaact
    40501 aagaggagga acagaaaatg aaagaagttc aattgatcag aaaaagcgaa ctatcagaag
    40561 gtggttgcaa tgcatgcggc gtagtagaag caacaagcta tacattaaag cttggtgcca
    40621 acaaagcgat aatctctgag ttgacagtag gcggtcttgt ggacagtctg gcgcttgctg
    40681 aaggttttat tggagaagat atttatgaga tgttttcaga aatccgtcag ttaaaaaaag
    40741 gggaaaactg catagaggtt catcatgaga gcccgaatgt gagattcaaa cgtggtgata
    40801 atgagatgat cttcaataat catgtgtccg atcacaccga gctatatgga atcgtgaatc
    40861 aaatactaac tgagcttttt ggattgggac cttatgcgtt caaggaagaa aacggtaatc
    40921 caaaattaaa cgaagaatgg caagaaacga ttgaaataca aagaaacaac ccgcacttat
    40981 ttcaataggc atccacagta tttcgtaaaa gggactttga cacgactttt gtcaaagtcc
    41041 catcgttttt tatctatttg tgttctaaac atttctttca gagtagtaac aactggaatg
    41101 actgaaatgt aaacagatta tttgcctgta tcaatacgga agagtaatat aaagtgtgta
    41161 aacgctatca tattattcat tcaatttttt atagagggaa ggcattgaaa tggaaaaaac
    41221 gtatcaagca ggtacaaatg aaggaatagt ggattttatc aatatggaag atttagaaat
    41281 agccgcttca caagtgattc cagctggtgg ttatggttac atcagcagtg gtgctggcga
    41341 cttatttact tatcaagaaa atgaacgggc attcaatcat cagctgatta ttcctcatgt
    41401 attaaaagat gtagaattac ccgatacaac tacacatttt gacaaagaaa cgctgactgc
    41461 accaatcatc atggcaccag ttgctgctca tggcttagct catgtgaagg cggagaaagc
    41521 ttctgcaaaa ggtgtagcag attttgggac gatctataca gcaagttctt acgcttcttg
    41581 tacattagaa gagataagag aagcaggcgg ggagaaagca ccgcaatggt ttcaatttta
    41641 tatgagcaaa gatgatggga tcaatttgga tattcttgaa gtggccaagc gtaatggagc
    41701 aaaagcaatc gtattgacag cggatgcaac agttggcgga aaccgtgaaa cagaccgcag
    41761 aaatgggttt accttcccac tgccaatgcc gatcgttcag gcttatcaat caggagtagg
    41821 ccaaacgatg gatgctgtgt acaaatcttc caaacagaag cttagtccaa aagatgtaga
    41881 atttattgct gctcattctg atttgccagt ctatgtgaaa ggtgtccagt cagaagaaga
    41941 tgtctatcgt tcattagaat ctggtgctgg cggaatctgg gtatctaatc atggtggccg
    42001 tcaactagat ggtggtccag cagcgtttga ttcgttgcaa tatgtggcag aagcagtaga
    42061 taaacgtgta ccaatcgtgt ttgacagcgg tgtacgacgc ggacagcatg ttttcaaagc
    42121 aatcgcatca ggtgcagatc ttgttgctat tggcagaccc gttatttatg gattgtcttt
    42181 aggaggaagt acaggagtcc atcaagtgtt tgatttcttt aaaacagaat tggaaatggt
    42241 catgcaatta gctggaacac aaacagtaga agatatcaaa aaaataaaat tgcgtgagaa
    42301 tcgttttatt taagaaacaa atgacgaaaa aaataggagt gcggcagatg atgccgtact
    42361 cctattttta tagttcaagg atgtcactaa tagaataatt tgaaacgaaa aaataaaatt
    42421 gaaatatttt ttcatactta ataaaaaagg aagaatattc atgtaaacga ttttattgtt
    42481 tttttctctt ttatacaaaa agggtcataa tttcaagtaa gcggatgaat tcatgaggat
    42541 tttttgcata ctgtctctac tatacttagg tcactaagaa aaaggagtga tttattatga
    42601 aaaagaacat gattgggaca ttattacttt caacacttct tctaggcggc ttggcaacac
    42661 cagcttttgc tgagggtcaa gcgacttcaa aaggagatat tacctttact gaaccaacaa
    42721 ataccgtgga gcctcttaac cctactgatc cttcaaaacc agtagagcca gcagatccgg
    42781 aaaatccagc aactggccag acaggtcctt taacactgga tgtagttccc gaattgccat
    42841 ttggtacaca cgagattgaa agcggaacaa aaacgtatca agtagacgct tcaaaaaacg
    42901 atacacctta tctccaagta tcagatcgtc gaggtgtagg tgcagatggc caagcacaag
    42961 ggtggaacgt gacagtttcg gtttcagatt ttgtaaacgg ttcacaagtt ttgcaaggcg
    43021 ctgaattaga ttttggaaca tccactgtta aaagtacgtc agataacgaa tccactggac
    43081 caactagtca aactgtaact gggttgagta aagcaagtgc agcgacacca atatttacag
    43141 cagcaaaaga tcaaggtctg ggaacatggc tttctgttta tgatccagca aatatcacac
    43201 tgaaagtgcc gaaagcagca gctggaacat tcacggctga tttgacttgg aacctcgtgg
    43261 ctggcccagt agcatagagt atgaaataaa ttcaacaaac tgcggtgaaa atactatagg
    43321 tgaaggattt tttaccgcag tttttgttga aaggatgtgg tggacaatga atcaaagtaa
    43381 acgactgttt gtttcgtttt tttctatttt aagtctattt ttcatttttc cgtcaatcag
    43441 ccaagcaaag gattcagcag gagactttgg aatcaaaccg gtatttccag agaaccaaat
    43501 agataaagca atcggttatt ttgatttatt agtggcaccg gaacaaaatc agacactaga
    43561 agtaattatc agcaattctt cagatgaaga acgaacattt gaggtctctg tcaatccggc
    43621 ggtaacgagt gatggtggaa cgatcgatta tagtcaaaaa aacccaacgt tagatgagac
    43681 attgccgttt gatgtccgtg acgttttgct cattgcgaaa aaggaaatca atgtatcagc
    43741 acatgcagaa acaactgtac ctatagaagt gaaaatccca gcaaaaagct ttaagggacg
    43801 tgtattagcg ggtattcatg tttcacctaa agaagaagct gaaacagaaa atgctacaga
    43861 aggagcacaa atcaaaaatc gtattgctta taatctcgca gtcgttttac aagaaagtca
    43921 agagacgata gaaccggatc tgaaactttt atcaggagat ttggacgagg tcaacgcaaa
    43981 gccaactgtt cagcttcgtt tccaaaatcc tcagcctagg attatcagca atttgatatt
    44041 tacgagcaag atcttttatg aaaatcagct ttatattgaa aatacgtcta atgcattttt
    44101 agttgcacca aattcgaatt ttcacttgaa tcttgattta gctggtgata aagcaaaagc
    44161 cggcgattac cgcgcagaaa tcatagcaaa atcaggcgac agtaatgaat ggcgatttac
    44221 acaaaacttt acgatcaaaa aagaaaaagc acaaaaagta aatgaaaatt ccgtttttgc
    44281 agtacaagaa caatctttcc catggcttta tgcaggattg gttgctgcag ttctagtgtt
    44341 atggttgatt ttgctgtttt tgtgggtcca caagaaaaag aaaaaaggag aaacaacatg
    44401 atgaggtgat ttcgttgaaa acatcgatct tattgggagg gattattgca agtttgctgt
    44461 tcttcagctg gcaagagcca gtccaagcag ataccaaaag ttcaggtacg attacatttt
    44521 atgatctaag tccaattccg catccaagtg gttcagtcaa tggcgcatca aaacagcaga
    44581 cacagctacc gatttataaa gaatctaatc aacatggcgt attcccggag acgaatgaac
    44641 aaaagatccg ttttctttat ttgatcattg gtatagaatt atggcttctt gcgtggttgt
    44701 tgttatggag aagaaaggaa aaggaggatg aacatgttgc tacaaaataa attttcattt
    44761 tttcgctcct tcttttttac ttcgcttttt gttttaagta tatttatctt ttcaccttcg
    44821 gcagaagctg ttggagcaac gcctcctgcc atttcttatg atccatacac gtatgaaaat
    44881 atcgtacctc caaatggtgg acgtagtgca gtcaatggcg ggaagcttgt ccaactaact
    44941 ggggctaacc aattgcctaa attgactgct acttatttga acgatggttc aaatgatcct
    45001 tggaaaggtg gcacggtccg ttttcattgg gaaagccatg tgaaactaac aaatgtactt
    45061 gatttggtga atatcactgc acaaggattt gcatttgtgg cgatcatcaa tttgccggaa
    45121 catgtatcaa gcagtgatgt attaaaagca attgactgga acaacgccta cttgtatatt
    45181 gccggtgccc ccatcaaggt tactagcgga agtttattat cagcaggaag ccataccttg
    45241 cgttttggga tggggtcgtg ggatacaaat actctattga cagatattat tgctattatt
    45301 acgcgaggcg aattcaacgt ggacgatatc ccgcttcaat ttgatgttac agctaatgtc
    45361 gcgaagatga cagaaaacgg agataaatat gatacgagtc caaacaaaat tttgaccaag
    45421 ggtaaattac aaccttctat gtctaaaaag gccgtttttt ctgttgattt ttatgattcg
    45481 aataatatta tccaagatcg tatgttcaat tcgattttag ctccagctgc aggtagattt
    45541 tatcctaaat taactggcgg aaacttgaat ccaggagcat atgcattttc tgcaagtgca
    45601 gagataaata cgtggaatag ttacatctca ccaagtgatt caacaggaaa atataataca
    45661 tttgctgata ccacagaata cgtggatgga aataaccaga accttccagg ctctaccaat
    45721 agtcggacat tgacgatggg gctgactgaa ggacgttttg atcagttgcc atttagtcgc
    45781 tttgatcgcg tagtcaacta ttttacagga aaaaacgtta cacaaggagc aatactgact
    45841 catactccag caagcacaag tggattgaat cagacagcca atatcgtgta cagtgggaaa
    45901 gatgcgcaag gcggattgct ttctcctgtt gctttaaagg tattggagca gtacaagcta
    45961 gacggaacgg ttttaccaac aaacttaaca gccgatagtc agtggggaaa gctcctttct
    46021 tctgcatcgt accaagtcac ggccaattgg tcagcaactg aactaaaaaa gggttcgatc
    46081 cattacaggc tttattcgaa aggaacccaa aaattagcga caggatggac agatcaactt
    46141 ttccaaacga tccaatcaaa taacgggggt gtaaatacag ctacttctac actacctgca
    46201 ttggcttcag ggcaatatta ttttgattat cgcttggtgg acgatgtact tactaacatg
    46261 tatccgtctt ttgcgtacaa atggcagtct gaacaagctg gaatcaccac tcttcctcaa
    46321 ataacagtgg ctaattttcc atctgtgagt gcagatagtc agctaaaaaa ccttagcaga
    46381 caacctgaga caggagatcc cctgtctgct ctatcaggag atacgattca agaaaatgtg
    46441 acattcacgt taacaaaatc tggggatgcg ataaccgaca aaaaaatcac gatctcactt
    46501 cctgaaaata ctacttatat caatggttcc ttaaaattaa atggtacaac tatcgcagat
    46561 accggcattc aacaaggagt ttctgtccca gcagatctat tgagcaaaat aggagatacc
    46621 attcatctta cttacaacta tcaattaaat actgtagata cttctttaca atccgtcagc
    46681 atactgacaa aggcagctgt actttccagc aatattacat tagcagatgg tgctaagctt
    46741 cctaacccag ttgtccaaac atctgctaaa acaattcttg tccccaaaca agaattaaca
    46801 ttagttaatg taccagatga ttttacattt ggtaacgatc tccccaaacc gttgaagaca
    46861 agttattatg aatcaaaagg cgatttttct tttgatgtgc gggatactcg gcttccaagc
    46921 acgtctcctt ggcaactgac aggtacattg acttctttat ttaagaatga tcaaggtcaa
    46981 gaattaagcg gaacaaaatt atacttcaat cattctggat caaagcaatt gattcagcaa
    47041 ggtcaaaaca cgttgattta tgagagtgat ggaacagcta aaggagaggt tctcgttgac
    47101 tttcccgata ccgatggttt actcttagag gtaaatagca gtacgaatgc tcaacctggc
    47161 gcaacgtatc aaggcatggt cacatgggaa ctgacagctg ggccaaccag ctaagtgatt
    47221 agaatttgta tggatctgtg ataactgtca cagatcctct ttttcaagga tgagaaggaa
    47281 acttctgctg ttaaaatcag aaatgcttga aggagtggac ttggaacttt tcagtttgct
    47341 tgaaaaaaat gaacaacggc aaatcaaact tttccaactt ttattagagg aaaaaggaga
    47401 ggtttccgtt aataacgtac ttacccgctt atcagttgat cgggctacct tgaaagaaga
    47461 tctgttttat ttacaagcta acttggaaaa tatcaaacaa ttggaaattg ttatccaaga
    47521 agggaatatt ttattaatca gacatggaaa tatagcagca gtcgatgttt attatcattg
    47581 ttacttgcgc tattctacaa aatatcagat acttctttat ctgttggaaa atggctcatt
    47641 tgatcggcag aaacttgtca tggaacttaa tatcagttcg gctactttgt ctcgcagagt
    47701 gaaggaatta aatgattctt taaatgagtt tcatctgaaa ataaaaagcg ggcggctgac
    47761 cggtccagaa agtcaaattc gttattttta tttccaatta ttttggtttg gacgtcccta
    47821 tcttgaaaac aaaaaagaat acagtgattt atcaggaaac acttttttag atctgctgca
    47881 aaaagagttt agttttgcta ttacagaaga aggacgattg aaatttgaaa tatggctttc
    47941 cattatgaag aagcgaatgc gctgtacgaa aaaatccaaa ccagattata gcaatcttcc
    48001 tgttggattg tttcaaacga cacaattttt attggacctc catacatttt tgacccgtta
    48061 cttctttcaa tatgcctttc cttggaatca aaatgaaacg ttggtttttt atcttttcat
    48121 gatcagtaac tttacattag atacacataa ctcgcatgtc caggagtttt tgataagcga
    48181 aaaatacaaa gacgacgtag tagctcaatt gaatcgaacg tttaaacaat cgatagaaga
    48241 atatttctct ttttatgttt ttccagaaga gatttcgcaa aagattgcgt tgacagtgat
    48301 gcaaaatcac gctcattttg cctattttag aggatggatg aatatttttg gccaagaagg
    48361 attaaaacag cggttaactg aagtagaaga taaacaattc ctggttttat gtgaaagatt
    48421 ggtcagacaa acatttgcta ttgttgattt tactgaagta gacgaggaaa atgcccgctt
    48481 tgaattattt gctcgctatg catctatttt acggacgctg atcaagcagt tagaattacg
    48541 tctgcaaatt gcttatgatc ttccttatga aaggctaatg gcggatatcg tgaaagaaat
    48601 catccaggaa caattgctga atacgatcaa atatgattta gtcgaatacg agaaagaaaa
    48661 gcagtatgat atcattttga ctagccaatt gaaagagtac ccgtcacaga agtccgctaa
    48721 agtttttgta ttcactagca acgaatataa ttatgatttc ccctatttga accaattttt
    48781 aaaagaatgt tatttagaaa aattgaattt aaggatgaat aaaacatgaa aatcgtgatc
    48841 attggtgctt cccatgaagg catcacagct gccctaaatc taagaaaact tcaaccgaaa
    48901 gcagaagttt tactgatcga tgaagatcat aaagatggac taggctatgt ttcaaatgga
    48961 atcaaccttt atttaaaagg aaagatacgt agtttagctg aagttgccca taacatgcgg
    49021 actcttcaag aaagtggggc aaaactgatc accgaatgga gagtaactga acttgatcca
    49081 gacaaacatc agctcatcct cgcatccaaa gaaggaaaaa acgagaccat aacctatgat
    49141 aaattaatcg tagcgacagg ttcctctcct gtcaccttgt ataaacaaat tgaagctgaa
    49201 aatgtctaca cctataaaaa tctagtgcaa tctaagcaag tacttgccgc tttaaaagaa
    49261 gcaaaagaag tagtgatctt cggtgctggt tacattggat tggaattagc agatgctttg
    49321 agaaataaag gatacattat tcatctagtt gattatatgc cgaatgtttt atcaagatat
    49381 ttcgataagg atatgatcaa cagttttcaa aatcagctgc aaactaagca aataaatttt
    49441 tatccaaatg aattcttgat cgattggaaa aaatcagaag agaaagtagt atccgtacaa
    49501 cttctgtctc aaacaataaa ggcagatatg gtcatttttt ctgcccaaac tcgtcctaat
    49561 acgacattgt taaaagaaaa agtggctttg tatgaggatg agactgtgat agtcaatgaa
    49621 tatctgcaga caagtgatcc ggacatctat gcgattggag atatcgtccc agtcagtttt
    49681 gacaaaaata aacggcatct ctttttacct ttagttactc gagctgtaca tatggcaaga
    49741 gcagtagcgc taactttgag tggtcagcca accgcttacg acctcagaca aaagataaca
    49801 gcagcagtca ttatagacta ctttttagga actgtagggc tgacagaaga tgaagcgcca
    49861 ttcttggaac aaaataccgg ttcgtgttca ggagaatttg atctatttcc acaatacgac
    49921 gaagaaaaca aaacagtaaa tgcaaagctg atctaccatc cggatacatt agaaataatt
    49981 ggcggacagc tgataagcca agaattctta ttatcagatt tgaacctgct tgcggatatc
    50041 gtgaaacaca aaactacgat tccacagcta gcagttgagg attttggatt tctgtctgaa
    50101 tacacctctc gttttcacta cctcaatgag ttagctttta aagtactagc taagagagca
    50161 aatagcaata gagtagataa aagactgtga gaaatatcac ggtcttttat tgtgtgggga
    50221 tataataaat attaatcaat cgagaataaa aaattcatat aatgaaatag cggaagaatt
    50281 ttttgagcat aatattcgcc aaaaatttat caagtgataa aattgttgta taaaatgatt
    50341 atttcaggag gaggagttaa atgtttttag cattaaatga aattatgcat tcaaagttac
    50401 gttatgcgtt agttgcaggg gtgatgtttt tgatcgccta cctggtattt tttttgactg
    50461 ggttagctta tggcttagct caagataatc gaacagcagt cgacaagtgg gaggcagatt
    50521 cgattgtgct ttcgaaagat gcaaattcaa atctagggat gtccatgatt actaagaaga
    50581 tagcagaaga agtcgaaggt gggaaagtgg cgtatcttgc ccaaacacct ggagtcgtga
    50641 ctagtaaaga cagtacagaa gaaggaaaaa tcaatgttag cttcttcggt atcgataaaa
    50701 atcaattcat catgcccaat cttgttgaag gaaaagcttt tgataacgat gatgaggcag
    50761 taggcgatat tagtttgaaa gaagaatatg gtctagccat aggagataca gttaagttat
    50821 ctggtagtga caagacgttt aaactaactg gctttacgga tcatgccaaa ttcaatgtct
    50881 caccagttct ctatacaacc atcaatgctt accaagaaat ccgcttcgaa aaagaagata
    50941 caagtgaaaa tgcacggatt aacgcaatag ttgtcagagg taaaatcagt aatttacctg
    51001 aagatttaga acagatcaaa atttctaaat tcatcaatga attacctgga tataatgcgc
    51061 aagtattgac ttttggattt atgatcggat ttttgatcgt tattgcggct attgtgatcg
    51121 ggattttcat ttatgtactg acgatgcaaa agattaatat ctttggtgta atgaaagcac
    51181 aagggatcac aggaggattt attgcgcgtt ctgtagtggc acaaacattc attttatcat
    51241 ttgttggtat tcttcttggt ctattaggaa cagtgggaac atcccttgtt ctacctgatg
    51301 cggttccatt ccaaagcaat tggctgtttt ttggcgtcat tagcttgttg atgttagtag
    51361 tagctgtgtt aggcgcacta ttttctgttc ggacaatcgt aaaaatcgat ccgttgaagg
    51421 caattggata agaaggagga agaacatgaa agcaatagaa tttaaagaag tagaaaaaag
    51481 tttccaagat ggcgaccaag tagttaaagc attgaaagaa acaaccatga gtatcgataa
    51541 aggggaattt gttgcaatca tcggtccttc tggttcaggt aaaagtactt ttttgacttt
    51601 ggcaggcgga ttgcagacgc ctagcagtgg ctctgtattt atcaatgatc aagaattcag
    51661 taatcaaaaa gaaagcgaac gcgttaaact acgttttaaa gaaattggct ttattttgca
    51721 agcttccaat ctggttcctt ttctgacagt aaaaaagcag ttattattag tagataagat
    51781 taaacatgaa catcgtcaaa aagaagcaaa cgaattattt gagcagcttg gcgtggataa
    51841 gttgatcaac aagtatccag aagaactatc tggtggagaa cggcaaaggg tcgcaatcgc
    51901 tcgtgcattg tacaatgatc cgtccattat tctagcagat gaaccaactg ctagtttgga
    51961 ttcagaaaag gcacgagaag tagtcgatat cttggcaaaa gaatcaaaag acaaaaataa
    52021 agcaacgatc atggttactc acgatacacg gctgattgac aaatgcgata gaatatttat
    52081 gatggaagat ggtgtattga aagaaaaaga ataaccaaaa agcaggtgga aaaatggtcc
    52141 atcgatacaa caaagataca cagcaaaaaa ttttgaaaga agcgactgac ttgtttatgt
    52201 ccaagggata tctcggcact tcaacaagag aaatcgcaca aaaagttggg atcacccagc
    52261 ccaatctcta tcattatttc ggtgataaag aaaaactata tacggcagtt ttagaaaacc
    52321 acttaaaaga tgttggaaaa gctttgagag aaatcgttca aacaagcgaa acgggatttc
    52381 aagtaacctt gacaaaaatg gcgcagtttt tgattgagac acatctggtc gatttgtttc
    52441 tgatgcttca cgatcttgaa agcaatcttt caaaggaaac aagagatcat cttttctttc
    52501 tatggaagaa aaattatcgt gaaccttttg aagaaatctt ttcagcaaac caatctgtgc
    52561 taagaaatgg gatttcaaaa gaaatcgcag caaggcattt tttcttagtg ttagcacctt
    52621 acatcactcg gtcgaaaaga cctgtggaag aaccgctaaa tgtggcgcaa ttgatcgatt
    52681 tatatttgaa tggtgtatta ggagaagcgt aaagctgtta tatagggaga aaatagttag
    52741 gtgaagaagc aagatatgac gaagatcgtt atatcttgct tctttatata ggtagttttt
    52801 cataaataca aatttgttat tctctcatga ttatcagggt atatttttat ttattatgtc
    52861 ggtgttccag gaagtgtttt aatttaataa cgaagaaatt aattgtttgt agaatgaagg
    52921 aaactattta ttgggtagat gaatcaccac gaatagtcaa aaggagattt caggatatgc
    52981 caaaaatgaa aaaaatcatt ttagcagtaa tgttattttc tacggttata tcttttacag
    53041 cccctttttt gcttacttaa gctgatttta agttttggcc cttagtctgg atctacgagg
    53101 tgagagtcaa gaatgaaaaa aataattgga gcttttgtat ttgcaatcat tatcattatt
    53161 atctgttttc aaatagatgt ttttggtcaa aaaaagatag ttggacaaac ggtcagcagt
    53221 acgaacagta cggttacaaa gacaaaggaa tcatttgatg atacagaaca agagacatta
    53281 atcaaaatcg gaaaaaatga ccgtacacta tatacagatg actcgctaac taccccactt
    53341 gcaactataa acggcggaga gctggcggag tttctttctg aaacaaaaaa tgcttatcag
    53401 atcaagacaa atgatggcta tactggatat ttagccctcg aagatggaac caaagtcaca
    53461 aagaatatac aaacaaaacc ggaagagcta tctgatgcag tgattgtatt agatcctggg
    53521 catggcggaa atgatactgg ggcattaagc aatgatgaac aaacagaaga aaaagatata
    53581 acattaagta cagccataaa agtaaaaaag gcgctagagg atgctggagc aacggtttat
    53641 ttgacccata ctacagatga attagtacag cttggagata tatgcgacta cagtgaagaa
    53701 aaaaaggcag atgtctttat cagtttacac gcagactcta cggaatatgc gaatgaagca
    53761 actggaatca cgacatatta ttactatgga caagaggaaa cattagcaca gacaattgct
    53821 gactccttca ctgatctgcc tctggattca agaggaatct ccactgggaa ctatcaagtt
    53881 cttcgagaaa atcttcaacc ttctatttta atcgagatgg gttatatgaa taatgatagt
    53941 gatttaaagg aattagtaac agacagttat cagcagcaga ttgctgcaag tttgacacaa
    54001 gcattgacca cttattttca gtaataaata agcccagcca aacaaaaata gctcctcata
    54061 tttttcgttt ggccgggctt attatcgcag atgcaagctt ttaaacaccg tttaaggaag
    54121 aggtcgtgag cctgccgttc aactctgagt cacaaggagc aatttcaaaa aacagcttct
    54181 catgtttgat gactattgtg acttgtgacc gaggagttgg catgagaaca ccgtttaaga
    54241 aaaaggttgt gaaatcaacg ttttgttctg agtattagag aaacaaacgg aaaaatagtt
    54301 cctcatattt tgccgtttgt tgagctaata ccgaggatca gatgattgaa caccgtttat
    54361 tcggaacaag ggactgtgac atgacttttg tcacagttcc attatttttt tagtagcttc
    54421 aaagcttttt ctgtattggc aaaatgaagt ttggcatatt tttcaagcat aggcatgccg
    54481 cctttctcca atagttttgc cgctacttgg tcagatttcg ttccagctcc tgacggaagg
    54541 gtgattccag cttcagctga tgctttgtct aactcttcca aaaagctggc acgactgatg
    54601 atagaagcag cagcaacagc taaatgatat tgttctcctt tagtaacgaa gtacagtttt
    54661 tcttgtaatt gatttttttc taaacgcgca tattttttaa aatttgcttc cggcgtaaac
    54721 tgatcgatca aaatcgcttc tggtttttct ggctcgattt tttttagcag caaatggatc
    54781 gcttgattgt gcaaagcgac tttcatccga acggcattgt attttggttg gatctcattg
    54841 tatttttctg gagataagac aagcagttta taggggatgg ttttttttaa gatgtcagca
    54901 agctggatga tttggctatc ttttaactct tttgaatctc taacaccaag tttacgtaat
    54961 tctgcaagct gatcacgttt cacataagca gcacatactg ttagcggacc gaaatagctg
    55021 ccatttccga cctcatcact acctaaaacg gataattgac tgatattttt tggcaaagaa
    55081 gaggctgttt ttgctggctt ggcttgttta tcctgtgcct ccccccattt atcagcttct
    55141 attgctgcat tcgtaccttg aaacatcact tttccggatg tataagcggt aatggtcgtc
    55201 ccgtcttttt ttgcagcaaa gctagtataa ggaatctgtt tatttaacaa ataagagcga
    55261 tagtatgctt tcatttgact aagttggtcg tttgttgctt tgataatgac gttgctcata
    55321 aaattctcct cgtttttata cataaaacta taccgcaact cacgactttt cgttttctaa
    55381 gagattgcga gacatagcat ttttttaaag tttatcatat aatagttaag tatgtgaaaa
    55441 atatttgtgg agaacggtga aagaaccaac ttcatggtac actaaataaa actttcatca
    55501 aataggagga aatgattcgt atggcgcacg aaaaaacacg ctacaaagcg gtgattgcca
    55561 accaaacata tactattatc ggacgggaaa ccaagcatca tatggatatc gtaacgaagc
    55621 tgatcaatga acagttagca gaattgaaac aattatcacc acaaatggat aatgaacaag
    55681 cagcaatctt gatggctgtc aatgcgcttt cggatcaatt gaagaaacaa gaaagaatat
    55741 tggagctgga agaagaaaca gcagaattaa agaaaaaaat gatcaaattt acagagcttg
    55801 aaaatcgtgt gaaacgcatt gaagcaatcg agaacgaagc tcgggaagta ttgaaagaaa
    55861 atggtcaagc agatcatacc attaaaaatc atgtggaagc acaacagatt ttaaacgaga
    55921 aacgaaaagg acaaattaaa caaaaatctt ctaattaaga tccttttatc aaaataagaa
    55981 aaatttaaaa aagttagaag agaaaggaac gtagcatgct gagtttgctg attttatttt
    56041 tattgctaat tgcgtttttt tctggagcac gtcgcgggtt tgctttgcaa atcatattcg
    56101 cgattggata tgtattgtca tttattgctg cccagcattt ttacaaacca ttagcaagtc
    56161 atttggaatt atacattcca tacccagctg tcaccccaac atcaaagtta gcattttttg
    56221 atcaggtatt tgcctttcac ctagatgaag ctttttatgc aggaactgct tttctgatta
    56281 ttttgctaat tggctggctg cttacccgat ttgtgggggt ttttgtccat ggattgacgt
    56341 atgtgccgat cttgagacag gtagactgga ttgcaggagg catcctgtca ctaatcatgg
    56401 cttacgttac tatatttttg attttgcagt tacttgcctt tgttccatta gatgtggtcc
    56461 aaaatcaatt cggcgagcat agtttcgctc gttttatcgt tgagagaacg ccatttctta
    56521 ccaacaagat gtatgagctt tgggtaacga atattttgaa ttaaggaaaa tagtgctgga
    56581 aaatatttcg ttttttgtac agagtagttt atttaagaag gctgcgtagc gtatgtccgg
    56641 cacagctctt ttttcatagt gaaagataag gtgataaaat gaataaccga atccttgaaa
    56701 cattggaatt tgaaaaagtc aaacagatgg tcagacaatt tgtagtgact gcacaaggaa
    56761 aagaagaact ggcagaattg gtaccagtga gtgagaaaca aacgatcaca aactggcttc
    56821 aagaaactga ggatggattg aaagtgcaac gtctgcgagg cgggatccct atacctaaaa
    56881 ttgaaaatat ccgtccccat atgaaacgga ttgagatcgg agcagacttg aatggagtgg
    56941 agcttgcaca agtttccaga gtattgtcta cgacaagcga attgaatcgt ttcattgatg
    57001 atctttctga cagtgaaatc gaatttgcac gtttgtatat gtgggcagat cagttagtca
    57061 cgatccctgt tttgagccgc cgtttaaagg aagcaatcga tgaagacgga cgcgtgactg
    57121 atgacgcttc tcctgagtta aagagcataa gacagaacat tcggcgaagt gaacaagctg
    57181 ttcgtgaaca gttagatggg atcgtccgtg ggaaaaatgc aaagtattta agtgatgcga
    57241 tcatcaccat gcgaaatgac cgctacgtta tcccagtcaa acaagaatac cgcggtgtat
    57301 ttggcggtgt agtccacgat caaagtgctt caggtcagac tttatttatt gaaccaaaac
    57361 aagtggtgga tttgaataat cgtttgcgtc aataccaaat cgctgaacga aatgagattc
    57421 aacgtatttt aagtgaactt tctgctgaac ttgttcccca tcgtcaagaa atcatccaca
    57481 atacttatgt gattggtaaa atggatctga tgaatgcaaa agcacgattt ggtaaagaag
    57541 taaaagccat cgtgccggga atcagtgaag acaatcatgt cgtcttgaaa caagcaagac
    57601 atccactgat cgatcaggaa aaagtagttt ctaatgacat cacaatcgga aaagactatc
    57661 aagccatcgt cattacagga cctaacacag gtgggaaaac gatcacgttg aagactttag
    57721 gtctgttaca actgatgggg caagcaggat taccgattcc tgcaggtgaa gaaagtcaaa
    57781 tcggtatttt tgaggaagta tttgcggata taggagacga acagtcgatt gagcaaagtc
    57841 tatcgacctt ttcttctcat atgacgaaca ctgtagatat tttatcaaaa gtcaatgaaa
    57901 aaagtctggt attatttgac gagctcggtg caggaacaga tccgcaagaa ggtgctgctc
    57961 tagcaatcgc aatcttagat gatcttggta aaaagtctgc ttatgtgatg gcaacaacgc
    58021 attatcctga attgaaagtt tatggatata atcgggcaaa tacgattaat gccagcatgg
    58081 agtttgatgt ggatacatta agtccaacgt atcgtttgct gattggcgtt cctggacgaa
    58141 gcaatgcgtt tgaaatctct agccgattag gtcttgatac agaagttatt gatgaagcga
    58201 agcagttgat gaatgatgag agccaagatt taaacgaaat gatcacagat ttagagaatc
    58261 gccggaaaat ggcagagacc gaatatctgg aaatgcgtca ttttgtttct gaggcacaag
    58321 aacttcacga tgatttgaaa gaagcttaca gctatttctt tgaagaacgt gaaaaagaaa
    58381 tggaaaaagc aaagaaaaaa gcaaatgaag tcgtgtcaga ggcggaagaa aaagcagaaa
    58441 aaatcattgc tgatattcgt aaaatgcaac agcagattgg gcaaggaaat gtgaaagagc
    58501 atcagttgat cgatgcaaag acccaattag caaatctaca tcaagaagaa actttgaaga
    58561 aaaataaagt attgaagaag gcgaaagaac aaaaaacgtt gaaaccggga gacgaagtgt
    58621 tggttacgac ttacggccaa agagggacgc ttttacgtaa aaacgggaat cagtggcaag
    58681 tagaaatcgg tatattaaaa atgaatgtgt ccgaagacga gctgacgcct gttgctcctc
    58741 aaaaagagcc gacacaacga gttatccatg ctgtacgatc tgaatctagt agtcacgtgc
    58801 cgaatcaatt ggaccttcga ggaaaacgat acgaagaagc gctaagtgag gtagatcaat
    58861 atttggattc tgccattcta gctggttatc ctcaagtgac tattgtccac ggaaaaggaa
    58921 caggcgcact tcgcaaggga attactgatt atcttaaaaa ccatcggagc gtcaaaagct
    58981 ttgagtttgc accggcaaac caaggcggaa atggtgcgac aattgttaaa tttaagtaaa
    59041 gcttactaaa ggtaagcaga tggttcgcta tccaagtata gaaatgctat actagtcata
    59101 gattttaaaa atggaggtct ttgatatgtc acaagtaatt acagataaga catttaatga
    59161 agaaacagat aaaggacttg tattgattga tttttgggca acttggtgtg gtccatgtcg
    59221 tatgcaagca ccgattttgg atcagttgga acaagaatat gatgaagaag aattcagaat
    59281 tgctaaaatg gatgtggatg aaaatccaga aactcctcaa caattcggta tcatgagcat
    59341 tccaacatta atgttgaaaa aagatggtca agtagtagaa aaagctgttg gtgttcattc
    59401 aaaagaacaa ctacgtcaaa tgatcgatca atatttataa aaacttacag caaaaataag
    59461 ctgagggtgg gactgaatcc agttccgtcc tcggtttttt tgcggttgag ttaaggttat
    59521 tcaaattaat attttttgag tataattacg ttgtcaggct ggattgtttt tttgacagac
    59581 atgctataat aactttaatt gaatatgaag gggagtacca gatgaacgaa cgaatcaaaa
    59641 acaaattagc tttacttcct gatcagcccg gctgttactt gatgaaagat aaaaatggaa
    59701 cgatcattta tgtcggcaaa gccaaaatat taaaaaatcg tgtccgctca tattttactg
    59761 gtagccataa tacgaaaacg gaacggcttg ttagtgaaat cgaagacttt gaatacatcg
    59821 ttacagaatc gaatatcgaa gcattgctct tagagatcaa tctgataaag aaaaatgatc
    59881 ctaaatacaa cattatgtta aaggatgata aaacctatcc tttccttaag ataacgaatg
    59941 aaaaatatcc ccgtttggtg attactcgta aggtactaaa agacaaagca ctgtattttg
    60001 gaccatatcc cgatgtcggt gcggcgaatg agacaaagaa gatcctcgat cgattatttc
    60061 ctctgagaaa atgcaaacct agtcagacga aggagccttg tctttactat catttgggac
    60121 aatgcctgtg tccttattat ttcgacgtag atccgaaagt ctatacgaaa atcgttgatg
    60181 aagtgaaaaa ctttttaaac ggaggacacg aaaaaatcga gtcagagatc catcagaaaa
    60241 tggaaaaagc tgctgaaaac atggagtttg aaaaagctgc agaataccga gaccagatcc
    60301 gtgccattga gacaatcatg actcgccaaa aaatgacaaa tactgattta ctggatcgag
    60361 atgtatttgg ttatgcgatt gataaaggat ggatgtgtgt ccaagtgttt ttcgtaagac
    60421 aaggaaagct aattgaacga gatgtttcaa tgtttccttt ctataacgaa gcagaagaag
    60481 atttcttgac ttacctcggt cagttttatc aggaaaatga gcatttcatt cctaaagaag
    60541 tcttgattcc tgatacgatc gacaagccca gtgtagaagc tttgttagct acgaaagtgc
    60601 tgcaaccaaa acgaggagaa aagaaaaaat tagttgaatt ggcgagtaag aatgcctcag
    60661 ttgctttgaa tgaacggttt gatctgattg ccagaaaaca ggaacggacg atcggagcag
    60721 ttgaacgttt aggaaacgcg atgaatatcc cgaccccaat acgtatcgag tcttttgata
    60781 actctaatat catggggacg aatcctgtgg ctgcgatggt cgtattcatt gatggaaaac
    60841 cggcaagaaa agaatatcgg aaatataaaa taaaaacagt caacggaccg gatgattatg
    60901 catcaatgag agaagtcatc tatcgcagat attcgcgagt gattcgcgaa gggttgcctt
    60961 tacctgattt gatcttgatc gatggaggaa aaggacaagt agatgtggca aaagatgtct
    61021 tagaaaatca attaggaatc gatattccta ttgctggcat ggtaaaaaac gataaacata
    61081 aaacaaatga gttgctattc ggtacagatt tggctgtagt accgcttgag cgaaattcac
    61141 cagagttttt cttactccag cgaatccaag atgaagtcca tcgattcgca atcactttcc
    61201 atcggcagct acgcagtaaa aacagttttg cttcccgatt agatgaaatt gaagggttag
    61261 gaccaaaacg aaaaaaaatg ctgcttaaag agttcaaatc gttgaaaaat atcacagcag
    61321 ccacgacaga agaattacaa gaaatcggac tgccgaaaaa tgtcgcacaa aacgtatttg
    61381 ataaattaca tcaggaatga ggaaagggca ctttacaaag aaagtaaagt gtcctatcat
    61441 tttatgtaca acaaaaagag cgtttacgtt ttgttggtaa ataataaatc gcttacgtct
    61501 ggtgtctaag gatatatgtg atttcttgaa gaaggagtga agaaatgtta gaagtcaagg
    61561 atcttgttaa aacttttggg aactacacag ctgtcgatca tgtctctttc caaatccccg
    61621 atggaaaaat catggggcta attggccaaa acggggctgg gaagacaacg acttttcgtt
    61681 tgatcttgga ttttttgaca caagaccaag gagaagtgtt atggaatggc catcatcttg
    61741 gggagaaaga ttatgatgtc atcggttatc ttccggagga acggggactt tatccgaaag
    61801 taacgattca agaccaactg ctttattttg cagaacttag agggaaatca agaaaagaga
    61861 ttgaaccaaa aatcgacttt tggatggaaa aatttcaggt aaaagggaaa aagacggata
    61921 aagtaaaatc tttgtctaaa ggaaatcagc aaaaagtcca gctgatcgcc acgttaatcc
    61981 atgaacctaa attgatcatt ttggatgagc cattcagcgg gttagatccg gtaaatgctg
    62041 aattgctaaa agatggaata ttggaattga aaaaacatgg ttcatgtgtc attttttcta
    62101 gccacaatat ggataatgta gaaaaaatct gcgatcattt gatcatgttg cgaaacggaa
    62161 aaatggtttt gaatggaaaa gtacatgaga ttcgtgaatc atttggccgt acgaagttat
    62221 tcttggaatc tggtttaagc caacaggaag tagaagaaat cgaaggcgtg aaaaaagtga
    62281 tccttcatga agacaatacg ttagagatta ccttagaaga tcctgaagct gggaaagaaa
    62341 tctttactcg tgcgactcag ttcggctaca ttcctatgtt taaccagcaa ccgccaactt
    62401 tggaagagat tttcaaattg aaagcaggtg aaatcgatgc gtaagttttg gattattacc
    62461 aaagatgtct atttaaaaaa tgttaagtcg atttctttct tgatcatgat attagtccct
    62521 tttatcatgc tgggtatcat ttatgtagca ggaaactttg ctcaacaaaa tagtgatgtc
    62581 aataaaatcg gcgtattggc tgataatgag caaatcagtc agcaaatgag ccaagtcaaa
    62641 acagatgatt ttgagttcaa agcagttgat tctgaagaaa aagcacaaaa agagctttcg
    62701 gaagaaaaaa tcgatgcgta tttagtagtc tcaactgaga atgataagat aaatgggatg
    62761 ctttatagcg aaaattcatt aggacaaagt acacaactac tgatacaaca acaactgact
    62821 ggcttgcaat cgatgcttcg agcgagtcaa ttaggcattt caccagaaga agtagctagc
    62881 ttgagccaac aagcaagttt gactcgacaa aaagtcaatt ttgattcaaa aggaaaaatg
    62941 acagtcggag aagataactc gacagtccaa tacgtcgtaa gttatgtagc aacgattgtc
    63001 ttgttcatta ttattttgac gtatgcgcag atcattgcac aagagatcgc ttctgaaaaa
    63061 ggcacgcgga tcatggaagt gatcctgtcc agtacgactg cacaaaagca tttttacgga
    63121 aaattaacag gtgttttact tgttgcatta acgcaaatgg cattatacgg tattattttt
    63181 ggtataggat acagtcaatt caaagatatg gaaatggtca aaaatatatt agacggtatc
    63241 tcattggata acttgattgg accattcttg tggtattctc ttctattcat gatctttggc
    63301 attttgattt tttctgtttt agcagcttta tgtggctctt tggtcaacaa agcagaagat
    63361 acagcaaaag caattttgcc agtgacttat ctatctctag gcggatacat gcttggattg
    63421 atactaggag cttcagatcc aaacaatatc gttattcgga tcacttcgta tattccattc
    63481 ttatcttcat atattatgcc gattcgctta gcaaatgaga cagtcggtat gggtggagca
    63541 ttgatttcct tgttggtact tgtagccgta acattcttat tgatgatctt gtctgcaaac
    63601 atgtacaaat cgaatgtact cgtttatagc gaaggcggat tatggagttc attgaaacaa
    63661 tcgatttcaa ttatgaggaa tgaacgaaaa aaagcataaa acaatcctaa aatacagaag
    63721 gaagctggac atctattgtt cccagcttct ttttgtatag acctatttta tttattttac
    63781 tatattggat atatattcat tgaaaatagt gtataacaac tttataatat tgagtatacc
    63841 aatttttgga ggcgttttaa tggaccgtta tttagattta ctttcagaaa aattcccctc
    63901 aacggaggat gtcatcacgg aaatcatcaa tctcgaagca attttgcagc ttccaaaagg
    63961 aacagagtta tttttgagtg atatacatgg cgaatttcct gcttttgatc atattctgcg
    64021 tatcggttca ggaaatctta aagaaaagat caaagacctt ttttctgaac aaatgacgga
    64081 aacggatatc agccaattca ctatttttac ggcttatcca gaatatgcat taacgactga
    64141 ttggtacaaa gaacaagata aatcgcagct gatccatcat ttgatcgatc ttttacggtt
    64201 tacgacagtc aaatataccc ggtcaaaagt tcgaaaagga ttgccgaaag aatatagcta
    64261 tatcattgag gaactcttgt atatcgatga ccgtatcaat ggaaaaaaag actatgtaaa
    64321 aaaaatcatt gaacaacttg ttttgatgaa ggaagaagaa aaatttttaa cgaaacttgc
    64381 acagacgatc caaaaatctg tcatcgatca tttgcatatc gttggagata tttttgaccg
    64441 aggaactcaa gcagccaaag tgatggatcg aatcatggat ttttcttctg tagatatcca
    64501 atggggaaat catgatattt tgtggattgg tgcgtattgc ggctctgagg catgtttact
    64561 gacactttta cggattgcag cacgctataa ttatttattt gaattagaga aagaatatgg
    64621 gttgaattta cgtccattgt tttcttttgc tcatcaaact tatcagaaga atccagtatt
    64681 tgcgcctaaa aacagtaaaa atctatccga aagagagcaa gaagaattag aaaaaatcca
    64741 tcaggcactt tctatccttc aattcaaatc agaacatcag ttattggatc gtcgacctga
    64801 atttaaaatg gacgatcgta aattgctgga aaaaatcgac tatgaagcat caacgattga
    64861 tttagaaggt cagctctatg agttgaaagg aacttgtttt caaacgatcc agccagatga
    64921 tccaaaaaaa ttcttggaag aggaacaaaa agtgattgat agtttgatgt attcattcca
    64981 gcactctttg agaatgcaaa aacatatgac gtttttgatc gaaaaagggg gaatgtatct
    65041 cacgaacaat cagcatgtgc tgtttcatgg ctgtatacct gtagacgaga agggacagct
    65101 cttagcattt gaattagacc aatcttatcg cggaaaagaa ctgctgggct tttttgaaaa
    65161 acagataact gaaagcgcaa aagatctgac tgcaaaagaa gatttagcaa ccgatttgat
    65221 ttggtatgcg tggagtgggc cattttcgcc tttattcggc aagagtaaaa tggcgacttt
    65281 tgaacgttat tttctcacag agcctcatac gcacgtcgag aaaagcaatc cttattatca
    65341 cttgcgagat gaagagtggt tcagtgaaaa aattttagca gaattcgaca caaaagaaga
    65401 agggtcagca atcattaatg ggcacacgcc agtaaaagta aaaaaagggg agtcaccgat
    65461 caaagcaaat ggaaaagtat ttgtcattga cggaggttta tccaaagctt atcaaaaaac
    65521 gacaggaatt gccggttatt cactattatg caattcttat ggtttccaaa tcgtgacaca
    65581 ttatccgttt ttgcctattg aacaacaatt tgcaaaaaaa tccgaccaaa caaatgtgaa
    65641 aaaagtaatc gaacagcaac taccacgcaa actcaatcgt gatacaacaa aaggtatgga
    65701 actgcagcag cagattaccc agctcaaaag gctgttggac tatcgaaagg acgattgaga
    65761 agaattccag tgaacattgt tccatagact ggagactgag acaaaagtcg tgtcgcagtc
    65821 tctttttccg gataaacggt gttcttctgc caactcctcg gtcacaagtc acactattca
    65881 acaaacatga aaagctgttt tttgaaattg ctccttgtga ctcagagttg aacggcaggc
    65941 tcacgacctc ttggtataaa cggtgttcaa aagccagttt ctcggtatta agccaaattt
    66001 atgcaaaaat gtgaaaagcc attttcacaa aatttttctt aatactcgaa actaaacagc
    66061 tttttcacaa ccccctcaga aagtctatta aaattcattc acaaagaaat cgaataagcg
    66121 ctgttcagat ggatgtacac ggtgggtaag ttccggatgc cattgaacac cgaggatttt
    66181 atgcatatca tctttgcttt gaaccgcttc aacgagtcca tcgttagaaa aagcgatggc
    66241 ttttaataaa ggtgaaagat ctttgactgc ctgatgatgg taagaattca caaaatatgg
    66301 atcggggaga aggttgctta acaaactgtc tgaaacaatc ttgacttcgt gagtcgtaaa
    66361 ttgcggggca gtgggctgtt gttcatgttt tactgaccac tttggatata atgaaagatc
    66421 ttgataaagt gtaccttcca aaacgacatt caatagttgg agtccccggc agactgcaaa
    66481 aattggtttt ctttgtttca atgcttccaa gatcagtgcc tgttcaaatt ggtctcgttg
    66541 aatatttgtt tccgttagct ttggatgagg atcttccatg taaagttgtg gactgacatc
    66601 ttgaccacct gcaagaataa gtttgtcgat ttgtccaatc agttgaggag ctgaatctgg
    66661 agacatgact ggcagaataa gaggcaatcc ttgagcatct aaaactgcat ccacgaatcc
    66721 ttgcggtgta tagctcactt gatttccttg gaaagtatct gttgctcgaa tcagttgatt
    66781 tccggcaatg ccgatgattg gtttcatttg tattccccct aaagtagact tattgtaaca
    66841 aggatttaaa aaaaattaaa ctatgtaaga cttgccccta tacttatctg ttcttgctgt
    66901 gttactatct gtaaagagaa atttcgtgtg aaattcatga cgatagttga aaatattgct
    66961 taatttgtta taataaaaag taagcagaaa taaaaaggtt gggcgtaagc ttcaaggatc
    67021 tagttcttca aggggtgaga aggacatttt attgaagggg cctgcctata ctaagttatc
    67081 tttagaggta catactttca atgaaaaaag aaagcatgta cctctttttt aaatcgaaag
    67141 gaagtgaagc aagtgttaga agctttggaa caaatccgaa aagcagaaga agaaaatgaa
    67201 aagcgtgctc aaacactgcg gcagcagctg aagatctatg caacagaaaa agagcaggaa
    67261 ttggaaacga tccaaacaaa ctatcgacaa caactaaaac agttggtgga agaaaaagaa
    67321 caatttgaaa tacaagaatt agaaaaagaa aaaatgaaat tgaaagcaga agcagacaag
    67381 gtgcaagcaa ctttgaaaga tcaatatcaa aagcatcaga gtcaagcgat cggtgcgatc
    67441 attgaaaggg tgaaagaaac ttatggccgt cactaagatg gaaaaagtga ccctcatttc
    67501 agataaaaga aatcaggaga ggattttaca agcagtacaa ggaatccaaa acgttgaaat
    67561 ccgggatctg tatcaaaata gaacaaacaa ccagtgggtg gagaagtatt ttccagatac
    67621 cacgattatt gataaagaag caaaattaag cgctttaggt aatcggttga aagacatcca
    67681 agaagctatc caattcatac aacaccacgg cgatagccgt caaaagaaac tccatttgaa
    67741 acgtcaagaa ctttccttac aagatttgga agccaattat tccgaagaaa atttcataaa
    67801 taaattgaaa gaagtccttg atttaaaaga aaagtgggaa caactaactg aagaacgtga
    67861 tcgtttagaa gaagaggaag aatggcttct tcattggcaa cagctggata ttgctccaag
    67921 tatgtatgca agtaagacga ccatttttga gatggggact gtgagtgcag caaattttga
    67981 aagtttcaag gaagaactga gtaagcttga tgacgtttat atggaagaaa tagattatac
    68041 gccaaaacaa gtttatcttt cgtatgttgc tttaaaaaaa tcagttgctg ttatagaaga
    68101 aattgctggg aaatacgggt tcaataaaga aacgtatcct tatgaacaaa atcctaaaga
    68161 aaaactgcaa gagaccaagc agcaattatc cattgttcac gagcagcaaa agaagataac
    68221 gacagcgtta ggtcagtgca gtggctatat ccaagaattt gaatgggtag aagaagtcac
    68281 acttgccgtt gcagaacgag aaaaaataaa agaacggttt atccatgcga cttatctgat
    68341 cgttttgcaa ggttgggtag atgcagaaga aaaacagaat ttgctgcatg tgttaaacga
    68401 aaccgtatca gaagaagata tatacgtcag tttcgaaaca ccaaactcag aagagattca
    68461 gcaggaagtc cctactaaat tgaaaaacca tccgcttgtc gaaccttttg aattgctgac
    68521 tgaaatgtac agtctgccaa aatatgaaga agtagatcca acaccttgga tgacaccatt
    68581 ttatcttgtc ttcttcggga tgatggtcgc agatgttggt tatggtttgc tgatgctcat
    68641 tgggacaata ctagctcaaa aactacttgt tctaccacgc gggatgaaac gtttcgtaaa
    68701 attctttgag attctctcga taccatcgat catctgggga ttgatctaca gttcattttt
    68761 cggacagtca ttaccaaaag aactgtttgg catccgcttg cctttcccaa tcctatctac
    68821 gacggatgac gtcaatacga ttttgatttt atccgtgatt ttcggtctga tacagatttt
    68881 ggttggatta tttgtggcag caaaggaaaa tatcaggaga aaagattact tgagtgctgt
    68941 cagtgatgga tttgcctggc aagggatcct aattgggatc gttattgcgt tggttggtgc
    69001 catgttgata aaaaatacag catttgttta ccttggcgct gggctatcga tcattgctgc
    69061 cttatgtatc ttgatcgttc cgattatcca aacgccgtct aaagtcaaag gtgctgccaa
    69121 aggtgcgtat aatctttacg gactgaccgg ctatatcggt gatttagtca gttatacacg
    69181 tttgatggca ttgggtattt caggcggaag tattggagcg gcattcaata tgctagttgc
    69241 tttcatgccg ccagctgctc gattcagtgt agggatcctt ttgatcatcg ctttacacgc
    69301 actgaatatg tttttgacac tgctaagtgc ctatgtccat ggcgcacgat tgcagtatgt
    69361 cgaatttttt gggaagtttt atacaggagg cggacgtgcc tttgatccgc tgaaaacggc
    69421 ggaaaaatac gtcaacatca atcacaagaa aaaaaacaat aaataattgg aggattttta
    69481 tataatgatg gattacttaa ttagtcaaaa tggtggaatg gtatttgcgg tattagcgat
    69541 ggcaacagcg acgatttttt cagggatcgg ttcagctaaa ggtgttggaa tgacaggtga
    69601 ggcagcagca gcattgacaa caagtcaacc cgaaaaattc gggcaggctc tgattctgca
    69661 attgcttcca ggcacgcaag gtttgtatgg attcgttatt gcctttttga tttttatcaa
    69721 cttaggaaat gacatgtcag ttgtacaagg actgaacttt ctaggtgctt ctctgccgat
    69781 tgcttttaca ggcttgtttt caggtattgc ccaaggaaaa gtcgcagcgg caggtattca
    69841 aattttagcg aaaaaacctg aacatgctac aaaaggaatc atttttgctg cgatggttga
    69901 aacatacgcg attttaggtt tcgttatttc cttcctatta gtgttgaatg cctaagcata
    69961 aaggaggaaa agcagatgaa cgccattgaa aaaatcatct ctcagatgaa cgaagcagca
    70021 gagcaggaaa gagcggcgtt agaacaagaa gagcgaatga agatcgatca aaactttgaa
    70081 caaaaacgta cgcaagttga aacagaacat caaaaacaaa aagaaaaaca aatcgaatta
    70141 ctcgaaaaaa aataccgtca gctaaggaat cgccagcaag tagaagtacg acaagaaaat
    70201 ttgaatgcga agcaggaatt tttaagacga ttgtttgcag atgcggtaac tgaaatggaa
    70261 aattgggatg aatctgaaca aatccagttt atcaaaaatg cattgtattc attacctttg
    70321 acaggaaaag tagcattcat tgctggagaa aaatcagcag cctatctttc tcagacgttg
    70381 ttggatgaat ggaacaatga acttcccttt atgatggttt taagtgatga aactgttgct
    70441 gatcaagcgg gttttctgat aaacgatcaa ggagttcagt ataattttct tttcagcagc
    70501 cttgtacaag atatccaagg aacgatgagt tttgagattg ccaatcaatt attcgaataa
    70561 ggagggatca gtgtatggaa taccacgaac taaatccctt gattcgcgga agagagcttg
    70621 aacttttgtc caatgagaca tttgaacgga tgattcaaac aaattctgtc gaagcacttg
    70681 gtgaaatatt gaaatccacg atttacagtc catatattta tgacggatat gaatatgact
    70741 tcgaacagaa tcttttaaag gagcagagcc gtctatttcg ctggttaaag gaatcagcac
    70801 cggagcctga aatcgtttgg atctatacga tgcgttatac tttccataat ctgaaggttt
    70861 tgacaaaagc tgagatgaca aatcaaaatc tggaccattt gtatatcgac gatggatttt
    70921 attcattaga aaccttgaag gatgccattc atacgcaagt ttcaaatgaa cttcctgaaa
    70981 gcattatgga cagtatacga gaagtacgtg attattttga agagtctacc attcttcagg
    71041 gaattgacgt gatttatgat cgacagtttt tgacagagca gcgacggctt gccgataagc
    71101 taggatatca agaattgctg acagaagtca ttgcttttat tgacttgacc aatatcacaa
    71161 cgatggctcg aggaatattg cagaaaagaa catatgggtt catgacgacg gtactttcca
    71221 gctcgggaag tatcgaaaaa gaagtatttt tgaactttgt agataaagat atggaagatt
    71281 acacgaattt tctattagag acagattatg ctgattttct gcgccctgtc atccgtgagg
    71341 gaaatattga tctcgtaaag cttgaacagt tgaaagatga ttatctaagt tcattgtatc
    71401 aagaagcaca aacccaggct ttcggaccac tgccactact agcctttttg aacgcaaaag
    71461 aggtagaaag caaaaacctt cgtttactgg ttatcggcaa aagaagccat ttcgaatcag
    71521 aaacaatcag aaagcgggtg agacaagtct atgacgcata agatcggtgt agttggcgac
    71581 aaagattctg ttttgccatt caaattgttt ggctttgatg ttcgttatgg tacaaccaag
    71641 caaaccgttc gtcaggcaat tgaagaaatg gcaaagaatg agtatggcgt gatttatgtc
    71701 acagagcaat gtgcagcatt gagcccagaa acgatcgaac gttacaagga acaaatgata
    71761 cctgctatcg tattgattcc caatcatcaa ggatcattag ggatagggct ggaagaaatt
    71821 caaaaaaacg tagaaaaagc agtaggtcaa aatatcttat agtatgaaaa ggagatagaa
    71881 attttgcaaa ttggaaaaat cataaaagtc tccggacctc ttgtaatggc tgagaacatg
    71941 tctgatgcca gtatccaaga catgtgtcta gtgggagatc taggagttat cggagaaatt
    72001 atcgagatgc gtggcgatgt ggcgtctatt caagtatatg aagagacttc tggaattggt
    72061 ccaggcgaac cggttcgctc tactggtgaa gctttatctg ttgaattagg accagggatc
    72121 atttcacaaa tgtttgacgg aatccaacgg ccattagata cctttatgga aatcacacaa
    72181 agtaatttct tagggcgcgg agttcaattg cccgcattgg accatgagaa aaaatggtgg
    72241 ttcgaaccaa cagtagaagc cggagaaacg gtgagtgctg gagatgttat cgggatcgta
    72301 gaagaaacaa aagttataaa acataaaatc atggtgccaa atgggataaa aggaacgatc
    72361 aaaacaatcg aaaaaggttc attcactatt gatgaaacag tatgtgtcat tgaaaccgaa
    72421 aatggtgaca aaaatttgac gatgcttcaa aaatggccag taagacgtag tcggccaatc
    72481 aaagaaaaat taaacccgga tgcacccatg atcactggac agcgagtgat tgatacattc
    72541 ttcccagtga caaaaggagg agcagcagct gtacctggtc cttttggtgc agggaaaaca
    72601 gtcgttcagc atcaaattgc aaaatgggcc gatgtggaca tggttgtcta tgtgggatgt
    72661 ggcgagcgtg gaaatgagat gacggatgtt ttaaatgaat tccctgaatt gattgaccct
    72721 aatacaggag aatcgttgat ggaacgtacg gtattgattg ctaatacatc aaacatgcca
    72781 gtagcagctc gagaagcttc tatctatacg ggaatcacaa ttgcggaata tttccgtgac
    72841 atgggttatg acgttgctat catggcagac tccacttcac gatgggcaga agcacttcgt
    72901 gagatgagcg gacgtttgga agaaatgcct ggtgatgaag gttatccagc ttatcttggt
    72961 tcacggcttg cggaatacta tgaacgatct ggaagagtca ttgcattagg atcagaaggt
    73021 cgcgaaggaa gtataaccgc aatcagtgct gtatccccgt ctggagggga tatttcggaa
    73081 ccagtcacac aaaatacatt gcgggtcgta aaagtattct ggggtcttga ttccagttta
    73141 gcccaaaaaa gacattttcc ttcgatcgat tggatacaaa gctattctct ttattcaaca
    73201 gaagtaggga agtatatgga tcatatcctt caagctgact gggcatccat ggtcaccgag
    73261 ggcatgcgta ttttgcagga agaacagcag ctgaatgaaa tcgtgcgttt ggtagggatt
    73321 gattcccttt cagataatga ccgtctgaca ttagaagtgg caaaatccat tcgtgaagat
    73381 tatttgcagc aaaatgcttt tgatgatgtc gatacattca cttcaagaga gaaacaattc
    73441 aaaatgttga agatgatctt gacctttggt gaagaaggac gccgtgcgtt gtcgttaggt
    73501 gcttatttaa gtgagatcat ggacggtacc gtatcgattc gtgaaaggat cggacgaagc
    73561 aaatatatcc cggaaacaga attagaaaaa ctagatcaaa ttattggtga aatcaagcaa
    73621 acgatgcaaa cgatcgtttc agaaggaggc atgagcaatg attaaagaat atcgtacgat
    73681 cggtgaagtc gtcggtccat tgatggcggt tgaaaaagtt gctggtgtca aatatgaaga
    73741 attaatcgaa gtccgtatgc aaaatggcga gattcgtcgt ggacaagtat tggaagtcca
    73801 agaagataaa gcaatggtcc aaatattcga aggaacaagt ggtatcaatt tacgtgactc
    73861 ttctgtacga tttttaggcc atcctttaga actaggtgta tcggaagaca tgatcggtcg
    73921 agtattcgat ggtttgggac gaccgaaaga taacggacca gagatcctgc ctgaaaagca
    73981 tttggatatt aacggagaag taatcaatcc agtagctcgg gattatccag atgagtttat
    74041 ccaaacaggt atctctgcaa tcgatcattt gaatacatta gttcgtgggc aaaaattacc
    74101 agtattttct ggctctggtc ttcctcataa agaattagct gctcagatcg cgcgacaagc
    74161 aacggtactc gattcatcag atgatttcgc cgtagtcttt gctgctattg gaatcacttt
    74221 tgaagaagct gaatttttca tggaagattt tcgtcaaacg ggcgcaatcg accgttctgt
    74281 catgtttatg aatttagcaa acgaccctgc aatcgaacgt atcgctactc ctcgtatggc
    74341 tttgactgct gcagaatatc ttgcttatga aaagggaatg catgttttgg ttatcatgac
    74401 agacatgacc aactatgcag aggcattgcg agagatttct gcagcacggc gagaagttcc
    74461 aggaagaaga ggatatcctg ggtatcttta cacgaactta gctactttat tcgaacgagc
    74521 aggaagaatc cgcggactaa aaggatcggt tactcaaatt ccgattctaa caatgccaga
    74581 agacgataaa actcatccaa tccctgactt gactggttat attacagaag gacagatcat
    74641 cttgactaga gagctttaca agagtgggat tcagccacca attgatgtac tcccgtctct
    74701 ttcgcggtta aaagataagg gaactggacc cggaaagaca agagaagacc atgcagctac
    74761 gatgaatcag ctttttgcag cctatgcaca aggaaaacag gcaaaagaat tagccgtagt
    74821 tttaggtgaa tctgctttat ctgatgtaga caagatctat gcgaagtttg ctgagcggtt
    74881 tgaaaaagaa tatgtcaatc aaggattcta cacgaaccgt tcgattactg aaacacttga
    74941 tctagggtgg gaactgctat cgatgttgcc aagaaccgaa ttaaaacgaa tcaaagacga
    75001 catgctggac aaatatctgc cagaagggaa gtgagttctg atggcacgat tgaatgtgaa
    75061 tccgacccgc atggaattga ctcgattgaa aaaacagttg acaactgcca caagagggca
    75121 taaattatta aaagataagc aagatgaact gatgcgtcaa ttcatcctgt tgattcgaaa
    75181 gaacaaccag ctgcgtcaag aaatggaaga gtctatgaca gaagcaatgt ctgattttgt
    75241 attggcaaat gcttcgatca cggaaccttt catcgaagaa ttatttgttt tgccggcgga
    75301 aaacgttgat ttgtctatcg tagacaaaaa tatcatgagt gtaaaagtac ctgtgatgaa
    75361 ttttgaatat gatgaacagc tgacagacac acctttggaa tacgggtatt tgaattccag
    75421 tgccgaactg gatcggtcaa tcgatcggtt tactaccctg cttccaaaac ttttggaact
    75481 gacagaaatc gaaaaaactt gtcaactgat ggcgagtgaa atcgaaaaga cacgtcggcg
    75541 agtcaatgca ttagaatata tgacgatccc tcaattagaa gaaacgatct attatattcg
    75601 tatgaagctg gaagaaaacg agcgcgcaga agtaacaagg ttgatcaaag taaaaaatat
    75661 gggtaccact gactaagtgg tatctttttt taaaagagga aaacgatgat taaacaaagc
    75721 aaacaaataa agaaacgttt gccggctgtt cagcttctcg cattaggatt ttttctcctg
    75781 attttagttg gcggcggcct acttacccta ccatttttta gtaatagtgg gcatgggaca
    75841 aattttatcg atgcgctttt cactgcaact tctgctgtct gtgtaacggg attgacaaca
    75901 ttgaatacag cagagcattg gaacagtgct ggtcaatttt tgatcatgct tttgattgag
    75961 atcggcggtc ttggttttat gatggttccg attctatgct ttgcattgat aaagaagaaa
    76021 gtcagtctca gcacacgtat tatattaaaa gaagcgctga atctagaaga agtatcaggt
    76081 gtgatgaagc tcatgcttta catcctaaaa ttggcctttg tgatccaatt gattggtacg
    76141 gttgctttat cttttgtatt tgtgcctgaa tttggctggt tgaaaggtat ctggtacagt
    76201 ttgttccatg caatctctag tttttgtaat gcagggtttg atttgttagg agacagttta
    76261 gccaatcacc agcaaaatgt ttatttgatc atggtggtat ctgctttgat catttctggt
    76321 gggttaggct tcatcgtatg gcgtgattta ctggaatatc atcagaaaaa gaagatgacg
    76381 attcattcaa aaatcgcatt aaccgttaca ggtacccttt tattaggcgg attcgttgtg
    76441 ttctttttca cagagcgcaa tggggcaacg ctagtggaag ggaatatgtt tcaacgattg
    76501 gcaaacacct tttttatgag tgtgacacct agaacggctg gatattattc agttgattac
    76561 ctccatatga gccatgcagg tctgatttta acgatgtttt tgatgtacat cggcggaaca
    76621 tctgggtcga cggccggcgg attgaaaaca actacgttag gtgtgttgct gatccagatg
    76681 cgttcgatgt tgaaaggacg tacgagagcg gaagcatttg gccgtaccat ccgacaaggc
    76741 gcagtgttgc gggcacttac tttatttttt gttacgttga cgttgtgtgt agtcgccatc
    76801 atgattttat cggtgactga aacgatacct aaaacttctg gcatcgaata tattgctttt
    76861 gaggtcttct ctgcgtttgg tacagtaggt cttacgatgg gattgacgcc agacttgact
    76921 gcaataggga aattgatcat tatagcgctt atgtacattg gacgagttgg tgtattgact
    76981 gtggtcttct ctctgatgac aaaagcacaa agaacacaat caaaatacaa atatccagaa
    77041 gaaagtgtca tgattggata atttcatctt ctgacagaaa cctagtgatt tagtaaaata
    77101 agaaaataaa ataatttttc ttgcttcttg gatactttaa gtgcaaaatg ttcgtttatt
    77161 ttttgaggca ctacccgttg ttgaacctcg ggtagtgcct cttttttatt gaaaggaatc
    77221 aataagattt gattccttta tttatcaata ggttataatt catatttttt tattttttct
    77281 tttacgatac ttagtccaga agctaaaatt tcaaggttat tagaggatag ggaaattcga
    77341 acgaatgcat ctggttgcgc acgtgtagta aagcgtctgg aatgataaag tcggataccg
    77401 ttagcaagaa agtcattctc aattatctta gggtcaatct caggatcaac tggaagaatt
    77461 cgatagtaag ggacacgatt cgttggtttt tctactttga aaagagaatc aaaaagctga
    77521 ttggctcttt ttgtaagact taatttagtt tctaagatcc tatttgcttc atttgaatgg
    77581 aggatttgag tgatgacttc cacatctaat ccagaagttt tcacatttat attaaaaatt
    77641 gcttgttcta tcttgctttg gacatgtact ggaactacaa gataagcaac tcgcaatcca
    77701 gaacaaataa attttgtcat tccagctaag tagattgttt ggttaggtaa taaattttgg
    77761 aaaggaggaa tgatcgtatg ttgatagtaa gttgttaaaa atgaatggat atcatcttca
    77821 ataacccata gaccttcttt ttcaatcact tcggctaatt gctctcgtct tttgtgagag
    77881 atttgaaacc ctataggatt attacaagaa ggcataagaa agacaccatg aatcttcttt
    77941 tttctacatt cctgaatcaa tatatcaggt cgcattcctt gctcgtcaaa atcaattgga
    78001 acgatctcta aatgatataa ctgagccaat tcaatgaaat tagcgtatgt atatcggtct
    78061 gttgctattc tatcacctgg agagaaaagt gcagaaagtg tgatagccaa tccattttga
    78121 acaccggaaa caatagatag agattctaag gacggaatga ctccttgagt ttttaaccaa
    78181 tttgctgcag tagtcaattg aaaatttgtg cctttgggat cttggtaatt aagcaaacca
    78241 acaagatttg ggttattact agttgattga ataaacggga caacgaattt attacattct
    78301 tcaaaagaac taaccagacc aaaatcaatg atatgttcgg ttacttcatt ggtagagatg
    78361 gtgatggact caaagacatt gggagaaata aatgtgccac ttccaatatt cgtatacagc
    78421 aaacctttct caattccata tttataagcc ttgccaacgg tagtaaaatt taaatctaaa
    78481 taatcagcta gctctctttg ggagggtaac tgagtgtttt tctgtaattt attctttaaa
    78541 atatcttgtt ctaattgttg aatcaacgat cgataataag gacgcttcaa tttttgtttg
    78601 tctggtcgcc aagttaacgg gtaattgtca taagaattaa taggcatttt ctccactcct
    78661 taaaattgta taccatacaa ttttaccatt gattgtattg cttttagaag ttaatctttt
    78721 gtgaggaggg aattgtctat gaaaattatt aattttgagg aagtagtagc ttcttttagt
    78781 tttgaagaag cgattcaagt aatgaaaaaa tgtttttttg attatgaaag tggtaaaatc
    78841 agccaaaatc ctcgattggt ggaaatttta cctgacggaa aaggcaaaaa tatgtttgcg
    78901 atgatgcctg cttatttggg ggagaacaga tattttggca caaaagtcat taccgctttt
    78961 ccggataatc atcagtatca tttaccttct catttaggag agattttctt gtttgatgct
    79021 caaaacggac tacccaaagc attgataaac gcaaatgcag tcacctggat tcgaacagct
    79081 gctgtatctg cgctggcaac agattactta gcaaatccgc atgcagaaac acttgcttta
    79141 attggagctg gtcaacaagc attgtcgcat ttgactgcga tttctaccat tcgtcccatc
    79201 actacagtgt ttgtttacga tattttagaa gaaagacgaa atacttttat cgctaaagct
    79261 aaaaaacagt atcctaaaat aacgtttata aatgcaatat cagtagaaga tgcagtacgt
    79321 ccagcggaaa tcatttgtac gttgacctct agtaaagagg catttcttca aaaagagtgg
    79381 gtaagggaag acgcacatat caatgctgtg ggcacattta ctcctgatac aagagagata
    79441 agtacatcgt tgatggaaac ttctcaaatc tatgtagatg attactcagc tgccttaaca
    79501 gaaagcgggg acctgcttat agccatggca gaaggagatt tagaggaatc ttgtattgtt
    79561 ggtacattag gcgaattagt ttctgagaaa aaaacatttt ataaaggaga atcgaggccg
    79621 acgattttcg acgcagttgg tctagctatt gaagatcttt gttgtgcaga atatatttat
    79681 aacaaattag agaaaggggc tttaaaatga acttagatta ttacgtagtt gatgcatttg
    79741 cagatgaggt gttcaaagga aatccagcag cggtttatgt gctagaagaa tggctgccag
    79801 aaggtacgat gcaaaaaatt gccattgaaa acaatttatc tgaaacagca tttacagtga
    79861 agaaaaacca agaatttgag cttagatggt ttacgccaga tcgagaaatc gatctttgtg
    79921 gtcacgcgac attagcaaca gcatttgttt tatttaacta ttacaaaata cctgatgaaa
    79981 cgattaaatt ttctactcaa agcggtaatc tatttgtgac aagaattaaa gattattatt
    80041 acatggattt tcctagtatt atgccgaaaa aagtgccgat attgtctgaa tatgaagaag
    80101 cgattggggc taagattaaa gaagcctact tagcacgtga tttatttttc gtattagaag
    80161 atgaaaaaac ggtggctaaa ttgcagccag attttacagc tatcaaaaat tttgaattag
    80221 gaataggagt catcgtgaca gcagaaagtg tgcaaaaaga ctttgtttct agaaccttct
    80281 ttccaaaatt aacaatcaat gaagatccag tatgtggatc agcacattct aatcttattc
    80341 cttattgggc agaaagaatg aataaaacaa aattgtcagc ttatcaaatg tcatcaaggg
    80401 gcggggatct agaatgtgaa ttaaaagaag atcgtgtgat aataggagga aaagcagttt
    80461 tgttttctaa aggtacctcg tttatttgat ttctttcaaa tgaattaaaa ataaattggt
    80521 ggagagtggc aaccttatag ctacaatggt tgttaaaaaa agacgcaaac tttttcaatt
    80581 taatgaaaag tttgcgtctc attttaatta ttggtgtttg ttttttattc aatcaagagt
    80641 aaaatgcccc atactaacta tcctcggatg acttcgattt tataaccatc agggtcaatg
    80701 acaaagtagt aagaaggcgc agtgccaggt aatcctttta agtcagtaat ctcaagacca
    80761 gcagctttat gttgctcgtg taatttttct agatcatcag ctgctatagc gatatgacca
    80821 tagccgtctc ctagatcata cgcaccatga tcatagttgt aagtcaactc taattcatag
    80881 tcatccccag gaagtgtcaa ataaacaagt gtaaatttgt tttctggaaa atcacggcgg
    80941 cggctttctt caaaaccaaa tgccttttga taaaattcga ttgatgcttc aagatctttc
    81001 actcgaacac atgtgtgagc cattttcata gattcatctc tccttttcct tttactcaat
    81061 cataacaaaa agaagtaagg aaatagttga tttgtgcttg ttcatgagaa atttctcatg
    81121 taaaatggat agaaaataaa actgttaagg tggaaactat tcatggataa accaattttt
    81181 ggtgaaaaaa agcaatttgc tgattatcaa acccgttatg cagcatatat tgtcatagaa
    81241 agagatcagc agattgcagt aatcgaggct ccaaatggtg ctttttttct tcctggcggg
    81301 gaaattgaag gagaagagac gaaagaagaa gcgattgtcc gtgagctgat agaagaaatg
    81361 ggaattgtgg cagaaatttc ttcttatctg ggagaagctg atgagtattt ttactcgaat
    81421 tatcgtgcaa cttattatta taatccagga tatttctttg ttgctgattc ttggaagcgt
    81481 ataggagaac caactgaaaa gaccaataaa atttggtggg tgagccctca agaagcgctt
    81541 gtgaaattga aacgtggtag ccaccgatgg gcagtcgaga gatggatcga aaaaaataaa
    81601 aactgaaaga aagcgagtag atcagctttc tctcagtttt tttatttatt acagtacata
    81661 ttttttgatt gcttcagcta caccgtgctc gtcgtttgat gaagtgatga catttgctgc
    81721 ttctttgaca agagaaacgg catttcccat tgctactcct agaccagcat attcaatcat
    81781 ggataagtcg ttctcgttat ctccgatagc catgatttca tcttgatcaa taccaaggtg
    81841 ctgtgcgaga ttcgcaactg ccgcaccttt acttgcttct ttatttagta cttcgaagta
    81901 atagggcgca cttttgaccg ttgtgtattt atcacggaaa gtttgcggta aacgcgcaat
    81961 tgctgcatct aaaatttctg gttcgtcgat catcatcatt ttgacgatgt tcatgtctgg
    82021 tgtcatttct tcaggtgttc gatattttag cggcatcttg actaaacttg cttcgtggat
    82081 cgtataagcg ctgatatctc gattaggcgt gtagatcgct ttgtcatcga tcgaatggag
    82141 atgactgccg acacggcgtg ccattacttc gatctctagg aaatcttcat gtgtcagtcc
    82201 gtgacgggcg atgattttac ctgatttcgt ttgttgtacg agtgccccgt tataagtaat
    82261 aacgtaatca ttatcttgaa aaaggttcaa ctcgtttaac tgttcctgga cacctggtaa
    82321 gggacggcca gtgcataaaa cgatcgaaac accttttgca acagcagcgg caatcgcttc
    82381 ttttacttct tttgttactt ctcttttctc atttaataaa gtaccatcga tgtcgatagc
    82441 gactagtttg atagacataa tttcctccaa tatattttaa tttatttcaa tcaatgcgcc
    82501 attacggata tggctggcaa attcttgata agtttcgtca aataaatcat aatgatcacg
    82561 caaggacgga tcaaccattt cttttggaaa gaaaaatcgc tcatctcctt gttcttgtcc
    82621 cgctaatgca gcaacaagct gactaacctg tgataattct atcaaagttc cgtcatttcg
    82681 caaaatctcg atttgtgtgc gatgtctgcc ttgttctggg cgataaaaat cataaggtag
    82741 atcgtagcta gaattcacag cagtgtaata aacaggatta tatccgacct tttctactaa
    82801 caaagtgagt tcttggacta attttgaatc ccggttgcca gaaaaagtag cagatttcag
    82861 tggtttacga ttcaaaaaac gtttagctaa atcatttaaa ataggatcag cagattcaga
    82921 ccattgagta aagtaagtgc ccaagacccc atcatcaagt ttgaggtatt cttctaaagt
    82981 aaagcttccg tcaagaaatg gcagaagtaa ttgagaatgg agttcaaaac tgctggcatc
    83041 ttcttgatac aactcgtgtg cgcgatgaag cagatgctcc aaaattactt ccataccacg
    83101 ggaaacagga tggaagtaca cttgcacata catttgatat cggctaacga tataatcttc
    83161 cactgcatgc atgccactca tggaaaaagc gatcccgcct tcataaggac gaatcacacg
    83221 taatattcgg gtaagatcaa atgttccata ttctgttcca gtgaaataag catcccgaag
    83281 caaatagtcc attcgatctg catcgatctg actagaaatc atttggacaa cttgtgggtt
    83341 ggggtaagtt ttttggatca cactagctac cttttcagga aaaccttctt ctactcggtt
    83401 aagaatctga tacacttctg tttctggaga agtgatgatc tctacagtga tggcttcatg
    83461 attcgtatgg aagatatgtt caaatgtatg agagtaagga ccatgaccga catcatgcaa
    83521 cagtgccgca cataaagtca ccagccgttc ctgatcatcc cagcctccgt tgcctatttt
    83581 ttcttttgaa aaatttcgag aaaagatatc gcagattcgt cttgaaatct cataaacgcc
    83641 taatgaatga gtaaaacggc tatgttctgc tccgtgaaaa gtgaaagaag aagtgcctaa
    83701 ttgtttaatg cgtcgtagac gttggacttc tttcgaattg atcaaatcta agattacttg
    83761 atgctggaca tggatatagt tatggactgg atcacgaaaa actttttcca taggcagcat
    83821 ttgatattta taaggggttg tcataatgta gctcctttat gttattacgt tgttccattc
    83881 tctggatttg gttgttccat tgctccagct ggatttcttt ttttagccag tcggtagaat
    83941 cctgtaaatc acttgcagaa tacagctttt caaaagcttt tgcaaatgct tctttgactt
    84001 cttttacaaa aaggggttgg tcaagcagtt cttcaagtgt tgccattgta gcaggatcca
    84061 ctgcaggata gccatttgta ccaaagtctt cttttaaagc agattgataa aatcgcttca
    84121 tgacttgccc acgtttttcc tgattaccgt tgacacttaa gtacatcata acagaaatcc
    84181 catttttgat ccgacgctga gcgataccgg caaatttttt gccatggatg cttaaatcat
    84241 aagttccagg acaataagaa tgggtgattt cgaatgcttg tatcggtgcc tttggaaaag
    84301 cgtatttagt caataggagc atttgctcgt aagcgtcatc tatggttatt ttttttgtgt
    84361 cactttgggg gaaaatcaaa gaaatattca aaattccttc atctgatacg atccctaatc
    84421 cacctgaatt tctgatcact ggagcgtaac ctgcatcttc cagtgtttgg atcccgtcac
    84481 ttagaaaagg agttcgaaca tccttcatac ctaagatgac tgtgcagtca tattgccaga
    84541 aatgtaaaaa gatttgctgg tgttctttag aatattccgt taatatatct gttaatgcaa
    84601 aaggggaaaa gtcgtttttt tgataaatgt gctgatctag tcgaatcgca gggttcattc
    84661 aaccggctcc tttgtataaa ttcatatttt ctacaatatt atactctaaa atcaaagagt
    84721 tgttttggaa aaaaggtttt ctgtaaaaat ggacaagttg gttgacaaaa caaaagaaac
    84781 taggaacaat gtagctatga gaagtaaagg agaatgaata atggaattat cacaaggaac
    84841 accgatttca atcaaattgc gtacaaaagt aaaacagcaa ggggaagtgc aagattttta
    84901 ttttgatttg aaagggcaaa tggtgacaat tggcgatacg ctgtacatac gttataaaga
    84961 gattcaggag gagacgggcg atgagattcc tgtaacgatc aaattagttc cagatggtca
    85021 tgtacagctg atccgtgcag gagaaatgcg gatgcgtttg aaatttggct ataaagaacg
    85081 gttagaaaca tcctatcgaa caccatacgg tatgatccag attgctactt ttacgaaaga
    85141 actgcatgtt agtttgaaag accgtcccac agcagggaag gttcgaatcg attatgatct
    85201 gttcatggga ccggaaaaaa tcggcgaata ttatttgacc ttggatttta cagcgtaaat
    85261 ataaaagaaa agtttgattt tttgtgtctg attttgtatg ataaggagta gactgtgaaa
    85321 ggacgtgtcc tgattggaaa ttagtgtatt tgaaggtgca aacaaaagcg aattatcaat
    85381 gatcgaagta gcacacgcga tcttagaaca acgtggcgat gtcatggatt tttctgattt
    85441 agctaaccag atccaaaact atcttgggaa atcagacagt gaaattcgtg attcactcgc
    85501 acaattttat actgacttga atattgacgg cagctttatc tcattagggg ataatcgttg
    85561 gggcttgcgt tcatggtatg ctatcgattc gatcgatgaa gaagtaaacc atggaatgga
    85621 cgaagaggat gaagatactc cacgtcgccg taaacgcaag aaagtcaacg cctttatcaa
    85681 tgatgatgaa gacgcgatcg attacaatga cgatgaccca gaagatacag atctgacaga
    85741 agaagatgac gatgatctgt ttgatgacga tgatgatgaa gatgaagaaa tcgctgctta
    85801 taattctgac ttgcaagaaa tcggagcaga tgacccgaca gatgatgaag aagacttgcc
    85861 aggaaatatc gaagaagatt taagtatcat agacgaagac gatgatgatg aagattatga
    85921 agaggaagaa ttttctgact ctgaaaaaga ataaacatta ttttcttacg agaggttgtg
    85981 acagagacga tcagcctatc agaataagga ggtgtgacac gatttttgtc ggctcttcgt
    86041 caaattgtgt tggcgaagac aaatttgaat aaaacttagt ccgatatgcg gcgcgttctg
    86101 tgcgccgcat atttttttag ttttcaaaga tcagaccttg ttgtaaatag atccgtgccc
    86161 gaaagttata aaagttgcga taaccaaacg caattcgttt aatcagcttg atcttgttat
    86221 tcagcccctc agtgacgccg tttgagtggg gcaccttaaa ggcatttgaa atacccgatt
    86281 ggtagcgatg gaagatcgtg aatttctttt taaacgactg aggtaacgta gatgaaattg
    86341 ttttaagggt ttcttgaaag ccttccgta
//
LOCUS       DAZAFF010000003        78861 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000003 DAZAFF010000000
VERSION     DAZAFF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.003"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            165..314
                     /locus_tag="ACG9BB_000186"
     CDS             165..314
                     /locus_tag="ACG9BB_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFN2836168.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            325..1839
                     /gene="dltA"
                     /locus_tag="ACG9BB_000187"
     CDS             325..1839
                     /gene="dltA"
                     /locus_tag="ACG9BB_000187"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFN2836169.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1836..3041
                     /gene="dltB"
                     /locus_tag="ACG9BB_000188"
     CDS             1836..3041
                     /gene="dltB"
                     /locus_tag="ACG9BB_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFN2836170.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3086..3319
                     /gene="dltC"
                     /locus_tag="ACG9BB_000189"
     CDS             3086..3319
                     /gene="dltC"
                     /locus_tag="ACG9BB_000189"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFN2836171.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3322..4590
                     /gene="dltD"
                     /locus_tag="ACG9BB_000190"
     CDS             3322..4590
                     /gene="dltD"
                     /locus_tag="ACG9BB_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFN2836172.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4731..5813
                     /locus_tag="ACG9BB_000191"
     CDS             4731..5813
                     /locus_tag="ACG9BB_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFN2836173.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5813..6616
                     /locus_tag="ACG9BB_000192"
     CDS             5813..6616
                     /locus_tag="ACG9BB_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFN2836174.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6686..7426
                     /locus_tag="ACG9BB_000193"
     CDS             6686..7426
                     /locus_tag="ACG9BB_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN2836175.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7437..8441
                     /locus_tag="ACG9BB_000194"
     CDS             7437..8441
                     /locus_tag="ACG9BB_000194"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFN2836176.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8486..10330)
                     /locus_tag="ACG9BB_000195"
     CDS             complement(8486..10330)
                     /locus_tag="ACG9BB_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836177.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            10541..11059
                     /locus_tag="ACG9BB_000196"
     CDS             10541..11059
                     /locus_tag="ACG9BB_000196"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN2836178.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            11187..11870
                     /locus_tag="ACG9BB_000197"
     CDS             11187..11870
                     /locus_tag="ACG9BB_000197"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFN2836179.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            12179..12252
                     /locus_tag="ACG9BB_000198"
     tRNA            12179..12252
                     /locus_tag="ACG9BB_000198"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12213..12215,aa:Arg,seq:tct)
     gene            12411..12770
                     /locus_tag="ACG9BB_000199"
     CDS             12411..12770
                     /locus_tag="ACG9BB_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFN2836180.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            12893..14524
                     /locus_tag="ACG9BB_000200"
     CDS             12893..14524
                     /locus_tag="ACG9BB_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFN2836181.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(14586..15227)
                     /locus_tag="ACG9BB_000201"
     CDS             complement(14586..15227)
                     /locus_tag="ACG9BB_000201"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFN2836182.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    15413..15489
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            15509..15817
                     /gene="rplU"
                     /locus_tag="ACG9BB_000202"
     CDS             15509..15817
                     /gene="rplU"
                     /locus_tag="ACG9BB_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFN2836183.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            15844..16188
                     /locus_tag="ACG9BB_000203"
     CDS             15844..16188
                     /locus_tag="ACG9BB_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFN2836184.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            16201..16494
                     /gene="rpmA"
                     /locus_tag="ACG9BB_000204"
     CDS             16201..16494
                     /gene="rpmA"
                     /locus_tag="ACG9BB_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFN2836185.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            16928..19735
                     /locus_tag="ACG9BB_000205"
     CDS             16928..19735
                     /locus_tag="ACG9BB_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN2836186.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            19926..20417
                     /locus_tag="ACG9BB_000206"
     CDS             19926..20417
                     /locus_tag="ACG9BB_000206"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFN2836187.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            20520..21509
                     /locus_tag="ACG9BB_000207"
     CDS             20520..21509
                     /locus_tag="ACG9BB_000207"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFN2836188.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            21542..22345
                     /locus_tag="ACG9BB_000208"
     CDS             21542..22345
                     /locus_tag="ACG9BB_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFN2836189.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            22366..23280
                     /locus_tag="ACG9BB_000209"
     CDS             22366..23280
                     /locus_tag="ACG9BB_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN2836190.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            23363..23737
                     /locus_tag="ACG9BB_000210"
     CDS             23363..23737
                     /locus_tag="ACG9BB_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFN2836191.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            23876..24121
                     /locus_tag="ACG9BB_000211"
     CDS             23876..24121
                     /locus_tag="ACG9BB_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN2836192.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            24293..25357
                     /locus_tag="ACG9BB_000212"
     CDS             24293..25357
                     /locus_tag="ACG9BB_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFN2836193.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            25603..26028
                     /locus_tag="ACG9BB_000213"
     CDS             25603..26028
                     /locus_tag="ACG9BB_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN2836194.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            26021..26470
                     /gene="nusB"
                     /locus_tag="ACG9BB_000214"
     CDS             26021..26470
                     /gene="nusB"
                     /locus_tag="ACG9BB_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFN2836195.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            26567..27421
                     /locus_tag="ACG9BB_000215"
     CDS             26567..27421
                     /locus_tag="ACG9BB_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFN2836196.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            27422..28759
                     /gene="xseA"
                     /locus_tag="ACG9BB_000216"
     CDS             27422..28759
                     /gene="xseA"
                     /locus_tag="ACG9BB_000216"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFN2836197.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            28764..28988
                     /locus_tag="ACG9BB_000217"
     CDS             28764..28988
                     /locus_tag="ACG9BB_000217"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFN2836198.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            28990..29880
                     /locus_tag="ACG9BB_000218"
     CDS             28990..29880
                     /locus_tag="ACG9BB_000218"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFN2836199.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            29889..30707
                     /locus_tag="ACG9BB_000219"
     CDS             29889..30707
                     /locus_tag="ACG9BB_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFN2836200.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            30814..31263
                     /locus_tag="ACG9BB_000220"
     CDS             30814..31263
                     /locus_tag="ACG9BB_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN2836201.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            31281..32963
                     /gene="recN"
                     /locus_tag="ACG9BB_000221"
     CDS             31281..32963
                     /gene="recN"
                     /locus_tag="ACG9BB_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFN2836202.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(33018..33134)
                     /locus_tag="ACG9BB_000222"
     CDS             complement(33018..33134)
                     /locus_tag="ACG9BB_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFN2836203.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(33207..34163)
                     /locus_tag="ACG9BB_000223"
     CDS             complement(33207..34163)
                     /locus_tag="ACG9BB_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN2836204.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            34334..34702
                     /locus_tag="ACG9BB_000224"
     CDS             34334..34702
                     /locus_tag="ACG9BB_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFN2836205.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            34872..35303
                     /gene="mraZ"
                     /locus_tag="ACG9BB_000225"
     CDS             34872..35303
                     /gene="mraZ"
                     /locus_tag="ACG9BB_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFN2836206.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            35324..36283
                     /gene="rsmH"
                     /locus_tag="ACG9BB_000226"
     CDS             35324..36283
                     /gene="rsmH"
                     /locus_tag="ACG9BB_000226"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFN2836207.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            36288..36689
                     /gene="ftsL"
                     /locus_tag="ACG9BB_000227"
     CDS             36288..36689
                     /gene="ftsL"
                     /locus_tag="ACG9BB_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFN2836208.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            36690..38882
                     /locus_tag="ACG9BB_000228"
     CDS             36690..38882
                     /locus_tag="ACG9BB_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFN2836209.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            38908..39870
                     /gene="mraY"
                     /locus_tag="ACG9BB_000229"
     CDS             38908..39870
                     /gene="mraY"
                     /locus_tag="ACG9BB_000229"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFN2836210.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            39884..41245
                     /gene="murD"
                     /locus_tag="ACG9BB_000230"
     CDS             39884..41245
                     /gene="murD"
                     /locus_tag="ACG9BB_000230"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFN2836211.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            41271..42359
                     /gene="murG"
                     /locus_tag="ACG9BB_000231"
     CDS             41271..42359
                     /gene="murG"
                     /locus_tag="ACG9BB_000231"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFN2836212.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            42374..43594
                     /locus_tag="ACG9BB_000232"
     CDS             42374..43594
                     /locus_tag="ACG9BB_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFN2836213.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            43750..45075
                     /gene="ftsA"
                     /locus_tag="ACG9BB_000233"
     CDS             43750..45075
                     /gene="ftsA"
                     /locus_tag="ACG9BB_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFN2836214.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            45098..46339
                     /gene="ftsZ"
                     /locus_tag="ACG9BB_000234"
     CDS             45098..46339
                     /gene="ftsZ"
                     /locus_tag="ACG9BB_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFN2836215.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            46356..47033
                     /locus_tag="ACG9BB_000235"
     CDS             46356..47033
                     /locus_tag="ACG9BB_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFN2836216.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            47047..47646
                     /locus_tag="ACG9BB_000236"
     CDS             47047..47646
                     /locus_tag="ACG9BB_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFN2836217.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            47727..47948
                     /locus_tag="ACG9BB_000237"
     CDS             47727..47948
                     /locus_tag="ACG9BB_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFN2836218.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            47997..48779
                     /locus_tag="ACG9BB_000238"
     CDS             47997..48779
                     /locus_tag="ACG9BB_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFN2836219.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            48800..49507
                     /locus_tag="ACG9BB_000239"
     CDS             48800..49507
                     /locus_tag="ACG9BB_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFN2836220.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            49769..52567
                     /gene="ileS"
                     /locus_tag="ACG9BB_000240"
     CDS             49769..52567
                     /gene="ileS"
                     /locus_tag="ACG9BB_000240"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFN2836221.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(52616..54142)
                     /gene="zwf"
                     /locus_tag="ACG9BB_000241"
     CDS             complement(52616..54142)
                     /gene="zwf"
                     /locus_tag="ACG9BB_000241"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFN2836222.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(54157..54804)
                     /locus_tag="ACG9BB_000242"
     CDS             complement(54157..54804)
                     /locus_tag="ACG9BB_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFN2836223.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(54988..55317)
                     /locus_tag="ACG9BB_000243"
     CDS             complement(54988..55317)
                     /locus_tag="ACG9BB_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFN2836224.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            55494..56222
                     /gene="sfsA"
                     /locus_tag="ACG9BB_000244"
     CDS             55494..56222
                     /gene="sfsA"
                     /locus_tag="ACG9BB_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFN2836225.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            56238..57251
                     /locus_tag="ACG9BB_000245"
     CDS             56238..57251
                     /locus_tag="ACG9BB_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN2836226.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            57251..58528
                     /locus_tag="ACG9BB_000246"
     CDS             57251..58528
                     /locus_tag="ACG9BB_000246"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN2836227.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            58591..61293
                     /locus_tag="ACG9BB_000247"
     CDS             58591..61293
                     /locus_tag="ACG9BB_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN2836228.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            61445..61762
                     /locus_tag="ACG9BB_000248"
     CDS             61445..61762
                     /locus_tag="ACG9BB_000248"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2836229.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            61792..62112
                     /locus_tag="ACG9BB_000249"
     CDS             61792..62112
                     /locus_tag="ACG9BB_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN2836230.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            62220..63680
                     /locus_tag="ACG9BB_000250"
     CDS             62220..63680
                     /locus_tag="ACG9BB_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN2836231.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            63748..63969
                     /locus_tag="ACG9BB_000251"
     CDS             63748..63969
                     /locus_tag="ACG9BB_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836232.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            64000..64182
                     /locus_tag="ACG9BB_000252"
     CDS             64000..64182
                     /locus_tag="ACG9BB_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFN2836233.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            64182..64595
                     /locus_tag="ACG9BB_000253"
     CDS             64182..64595
                     /locus_tag="ACG9BB_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFN2836234.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            64718..65899
                     /locus_tag="ACG9BB_000254"
     CDS             64718..65899
                     /locus_tag="ACG9BB_000254"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2836235.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(65970..66134)
                     /locus_tag="ACG9BB_000255"
     CDS             complement(65970..66134)
                     /locus_tag="ACG9BB_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836236.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            66288..66361
                     /locus_tag="ACG9BB_000256"
     tRNA            66288..66361
                     /locus_tag="ACG9BB_000256"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:66322..66324,aa:Arg,seq:cct)
     gene            complement(66430..67569)
                     /locus_tag="ACG9BB_000257"
     CDS             complement(66430..67569)
                     /locus_tag="ACG9BB_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2836237.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            67741..67875
                     /locus_tag="ACG9BB_000258"
     CDS             67741..67875
                     /locus_tag="ACG9BB_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836238.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(67868..68503)
                     /locus_tag="ACG9BB_000259"
     CDS             complement(67868..68503)
                     /locus_tag="ACG9BB_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836239.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(68616..69251)
                     /locus_tag="ACG9BB_000260"
     CDS             complement(68616..69251)
                     /locus_tag="ACG9BB_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFN2836240.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(69285..69746)
                     /locus_tag="ACG9BB_000261"
     CDS             complement(69285..69746)
                     /locus_tag="ACG9BB_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFN2836241.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(69876..70307)
                     /locus_tag="ACG9BB_000262"
     CDS             complement(69876..70307)
                     /locus_tag="ACG9BB_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFN2836242.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(70325..70645)
                     /locus_tag="ACG9BB_000263"
     CDS             complement(70325..70645)
                     /locus_tag="ACG9BB_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2836243.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            70944..71720
                     /locus_tag="ACG9BB_000264"
     CDS             70944..71720
                     /locus_tag="ACG9BB_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFN2836244.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            71735..71938
                     /locus_tag="ACG9BB_000265"
     CDS             71735..71938
                     /locus_tag="ACG9BB_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2836245.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            71954..72292
                     /locus_tag="ACG9BB_000266"
     CDS             71954..72292
                     /locus_tag="ACG9BB_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFN2836246.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            72279..72458
                     /locus_tag="ACG9BB_000267"
     CDS             72279..72458
                     /locus_tag="ACG9BB_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836247.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(72501..72971)
                     /locus_tag="ACG9BB_000268"
     CDS             complement(72501..72971)
                     /locus_tag="ACG9BB_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836248.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            73058..73756
                     /locus_tag="ACG9BB_000269"
     CDS             73058..73756
                     /locus_tag="ACG9BB_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFN2836249.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            73934..74275
                     /locus_tag="ACG9BB_000270"
     CDS             73934..74275
                     /locus_tag="ACG9BB_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836250.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            74268..74939
                     /locus_tag="ACG9BB_000271"
     CDS             74268..74939
                     /locus_tag="ACG9BB_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFN2836251.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            74945..75637
                     /locus_tag="ACG9BB_000272"
     CDS             74945..75637
                     /locus_tag="ACG9BB_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFN2836252.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            75634..76383
                     /locus_tag="ACG9BB_000273"
     CDS             75634..76383
                     /locus_tag="ACG9BB_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2836253.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            76395..76664
                     /locus_tag="ACG9BB_000274"
     CDS             76395..76664
                     /locus_tag="ACG9BB_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836254.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            76670..76834
                     /locus_tag="ACG9BB_000275"
     CDS             76670..76834
                     /locus_tag="ACG9BB_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836255.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            76827..77126
                     /locus_tag="ACG9BB_000276"
     CDS             76827..77126
                     /locus_tag="ACG9BB_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836256.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            77126..77428
                     /locus_tag="ACG9BB_000277"
     CDS             77126..77428
                     /locus_tag="ACG9BB_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFN2836257.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            77468..77833
                     /locus_tag="ACG9BB_000278"
     CDS             77468..77833
                     /locus_tag="ACG9BB_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836258.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            78323..78463
                     /locus_tag="ACG9BB_000279"
     CDS             78323..78463
                     /locus_tag="ACG9BB_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836259.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            78460..78642
                     /locus_tag="ACG9BB_000280"
     CDS             78460..78642
                     /locus_tag="ACG9BB_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836260.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            78655..78849
                     /locus_tag="ACG9BB_000281"
     CDS             78655..78849
                     /locus_tag="ACG9BB_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836261.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
BASE COUNT        26178 a        13137 c        16473 g        23073 t
ORIGIN      
        1 gtacgagaaa ttataaagaa aaaagcagtg atttgatgtg ctatttttat aaaagctatg
       61 tgacaaaact gttagtaaaa aaacggtttc tgtaagctag acacatgttt ctttttgtca
      121 aaattagtta cactttgtgt gaggttatat gaggagggaa tccaatgaac aaatggcgtt
      181 ctaatccaaa attgaattat tggggaaaat tcatcattcg aacaattttt tatagtgcag
      241 tactgctgtt cctgatttat ctgtatcact ataaaaatat tcaaggagga acttttatct
      301 ataacgaatt ttaggaggaa gagtatggaa atcaaaacga ttattgaagc aatcgatgat
      361 tggggaataa atgaacctga tcgagttgct tatcaggcgg atgagacgca tacttttggt
      421 gaattgaaac aagcatcaga cgcacttgct tattatttag aaaaaaaggt agaaggcgat
      481 ggaccaattg tcgtttttgg caatttggag tttgaaatga ttgtctcatt tctaggggtt
      541 gtcaaatctg gccatgccta tttgccgatt gaagagcata cacccaagga gcgtatccta
      601 tcgatctttc gggtggctaa accatctatg gtcatcagta tcggggactg gatagaagat
      661 attccttcgg ttcctgtcat aacaaaaaaa gaatttcaag aaatcaagaa gataccagta
      721 ggattttatc ccagaaatag cgtaaaaggt acagaaaatt tttatattat ttttacttct
      781 ggtactactg gagaaccaaa aggcgtacaa atcagtcatg ataatctagt cagctttgtt
      841 caatggacga tggaagactt tgggatcaag cctggtatgc atttcttagc ccaagcgcca
      901 ttttcttttg atttatcggt attttctatt tatcctgcat tagtttctgg cggaatgctg
      961 aagccgttaa ataaagcagt tgttcaagac tttcgtcaat tattcgcgac attgccagcc
     1021 ttgaagttga atgtgtgggt ttctactcca tcatttatgg atatttgctt gatggaacct
     1081 acattcaatg gagaaaatgt gccggaattg gacatgtttt tattttgcgg agaagaattg
     1141 acgaaaaaaa ctgcagaaag tctacttgat cgattcccaa atgcacggat atataatact
     1201 tacggtccaa cagaagcaac tgtagctatt tcaagtattc agattgacca gcatgtacta
     1261 gatagttatg accgactacc gattggctac gtaaaaaaag acacgaaagt ttctattgtc
     1321 caagaaggta aacctgtttc aaaaggagaa acgggtgaaa tcattattgc aggaccaagc
     1381 gtatcaaaag gttacctgaa caatcccgaa aagacagctg ctgcgttctt ttgtcaaaat
     1441 gacttagcaa gttaccatac aggggatgca ggtagactag atgaatatgg gcttttattt
     1501 tatgaaggac gtatggatca gcaggtcaaa ttacatggat accggattga actcggggat
     1561 atcgaacatt atctgctcca agacaaccga atcaaacaag ctgtcgtcgt accaaaatac
     1621 caaggaacaa aagtacagca gctggttgct tttgtcgtat tagaaaacaa aacgaaacag
     1681 ccggattttc agttgactaa atctatcaaa gaacaattac tagaagttgt aatggattat
     1741 atgattcctc agaagttcca ttatgtcgat ctgctgcctc agacagttaa tggaaaaatc
     1801 gatcgaaaaa aattaacagc tgaggtgaat cctgaatgat gttacctcat atgatccctt
     1861 atgcagagcc gttttatttt tttcttttag ccattgcgct tgtaccgatc gttctatctt
     1921 tattgatatg ggaaaaaaga ttaccagttt atcaatcagc agtgacactt ttctttttat
     1981 ttgtcagctt tggaggtaat tattggcaac agggagcggc gctgattggg tatgttttgt
     2041 ggcagacgat acttgtatgg ggctattttg tttatcggca aaggaaaaat cagacaggat
     2101 ggttttttgc cgccgttatt ttagcgataa ttccactgtt tttaacgaaa gtgactcctt
     2161 tttttgaaca tggaaaagca tcgttacttg gttttttagg aatctcttat ttaaccttta
     2221 aatctgtaca aatgatcatg gaaataagag atggcatgat caaaacatat aagccatttc
     2281 gttatatcca gtttctgctg ttctttccaa caatctcttc tggaccaatc gatcgctata
     2341 ggagatttga aaaagatcag ctccatccgc ctgttcgtga taaatatgtt gatcttttgg
     2401 gaaaaggcgt ccaaaatatt tttgtcggtt ttttatataa attcattatc ggttattatt
     2461 ttggtcaagt actgcttcct gtagtgggac gcattgcctt acagcatggt ggtatttcat
     2521 gggcattagt aggttatatg tacgtgtaca gtatgtattt gttttttgat tttgctggct
     2581 atagcttatt tgcagtcggt acaagctaca tgatgggata tcagacacca atcaatttca
     2641 ataaaccttt tttatcatgg aatatcaaag aattctggaa tcgttggcat atgacgctgt
     2701 ccttttggtt ccgtgattat gtatatatgc gattgatgtt cttcatgatg aagaaaaaat
     2761 tttttaaaag caagatcgta atgtcaaata tcggttattt tgctttgttt cttctaatgg
     2821 gtatatggca tggattgacg tggtactaca tcgtatatgg actgtatcat gcttgtctga
     2881 tctgtctgac agatgcatgg atccgattta agaagaagca taaagagcag ttgccttcta
     2941 acaaatttac gcatggctta gccgtatttt tgacatttca agcagtatgt tttagcttcc
     3001 tgattttttc aggattttta gataaacttt tttttagcta acgaatagtg acatattaat
     3061 ttatataaca aaggagaaaa ggaaaatgga agaacaaatt ttatcaatct tagaagaaat
     3121 aacaggaaca gatgaagtaa gaaaaaatcg tgatattgat ctatttgatg aagggttgct
     3181 tgattcttta gcatctgttc aattattagt cgagctagac ggagagctgg atatccaagt
     3241 ccctgtatct gaattcgagc gagaggattg gtgcacaccg aataaaatca ttcaacaagc
     3301 aactgcattg aagggttgag tatgaaaaag aaactttttg ctattttcgg cccggttgtt
     3361 ttagctgttg gcttactgtt tgtgtttttt acctcctcct atcaagtaga tatctctaat
     3421 ccagaaacga ttagacgtgc ctcttcttcc atgtcagtga acgtgttgaa aggagatgcg
     3481 ataaaaaatt atgcattatc tgaaaaacaa tatattcctt tttttggctc aagtgaatta
     3541 agcagaatca gtccatttca tccgtctgtt ctggcagaaa aataccagcg aaactatcgt
     3601 ccatttttgc tgggagcacc cgggacacaa tcgttaagcc aatatatgat gatgcgttct
     3661 gcaggagatg ctatgaaaaa caagaaagtc gtctttatta tttctcctca gtggttcgtt
     3721 aaaaatggtg taaaaactga ttactttaat acgtattatt ctgaacttca aacatacgac
     3781 tggttgtttt caatgaagaa agtaacgcct gctgatcgct atttagctag aagattatta
     3841 actttctcaa aagtaaaaga gaatgatacg ttgacggcga ttctgcaaac gatcaaaaaa
     3901 gggaagctac ctttacctga atctttgaat cagctgcgca gccaatggaa catgctgaag
     3961 agagaggatg aagtattcag caatattggg ttgaaagatc gacagcaaaa aatcgatcat
     4021 gagtcaaaac gattaccaaa acaataccaa gaaacagaat tatccatttt agcaaatcaa
     4081 attggagaac gagaaacgac gaataatcca ttcgggttga aaaacgattt ttatacgcat
     4141 cgtatccgag cccacgagcc agagctcaaa cagtcacaaa aaaattggga ttatcgtttt
     4201 tcgccagaat tttctgattt tcagctggtc ttggatcaat tagcaaaaaa ccataacgaa
     4261 gtattgttca tcatcccacc ggttaacgag aaatggagcg attataccgg actatctcaa
     4321 gaaatgttac aaggatttgc caaaaagatc aaatttcaat taaatagcca aggattcaat
     4381 cgaatagctg attttgtcaa tcaagcgggt accaactatt ttatggaaga cacgatccat
     4441 ctaggctgga aaggctggtt ggctgcagat caacagatca gaccgttttt agaagaaaat
     4501 catattactg ctagtaagta tcatcttgac gatgcttttt ttagcaaatc ttggcaacat
     4561 caaataccag ataaactaca attaaaataa agaaaacagg cgagtgttgc tcgcttgttt
     4621 tctttatttt tgaatattct gaataaaaga taaaaatacc aagtttgcct gaacaaaaat
     4681 cataagcaat ggtataatca caatatgaat atagaaatgg gggaaaacat ttggatacaa
     4741 aagaagaaca gctacttatt gatttaactt ctgcaaaagg agtacctgga aatgaagaag
     4801 aggtaagaga agtattcaga gaatatgcca aaccattcgc tgatgatatt ttttatgacg
     4861 gtttaggcag tgtgattgcc aaacatggtg ctggcggagg ccccaaggtc tttatttctg
     4921 gacatatgga cgaagttggt tttatggtaa caaaaataac cgaaaaagga ttcttggaat
     4981 tccaaactct tggaggttgg tgggggcaag tcatgcttgc tcagcaagtt gaaatcaaaa
     5041 cacgtgaagg gaaaatcgtc cacggtgtga ttggttccaa accaccacat gttttaacac
     5101 ctcaagtaag aaataaacct tatgaaataa aggatatgtt cattgatatt ggcgcatcaa
     5161 gccaagaaga agccaaagag tggggcatac gtcctggaga tatggtcact ccatatattg
     5221 aatacaaacg aatgaatggc agtaaatatt tactggcaaa agcatgggat aatcgtatcg
     5281 ggacagctgt ttctctgcga gtattagaga atctatctaa agaagcacac ccaaatgttt
     5341 tattcgcagg aagcgatgtg caagaagaag taggcttgcg aggtgctcga acaagtacac
     5401 accttgtaaa tcccgatatt gcttttgctt tagatactgg tacagcagga gacactccag
     5461 gaatgacacc aaaagaagca gattcgattt taggaaaagg cccacagatt ttgatttttg
     5521 atgcatctat gatcccgcat aaaaaattat tgaattttgt tattgatgta gcagaggaaa
     5581 tggatattcc attccaatat acagtgattt ctggcggagg aactgatgca ggacaaatgc
     5641 atttgacaag agatggtgtt ccgtctgtag ccattaccgt tcctgttcgt tatctgcatt
     5701 cccatacatc agtgattcat gaagatgatt atttgaatac agtgaaacta gtcacagaat
     5761 tagtgaaacg gcttgatagt gaaacagttg aaaaaattcg gagttatgaa taatgaataa
     5821 tagaatacct atttctgaat atcggaaaaa agcacgagtt tctttggaaa atcaatgggg
     5881 aatcaatact tggcttgttt tcttatctat aatatttacg agcgtcatcc agaacttttt
     5941 tgacggtttc agtccttttc cggaagaaag catgcagcag cacttattga gttttctagt
     6001 cgataattta cttttattcg cattcaacta tgcattgttt tacattgcgc tagtagttgt
     6061 acggggtggc agaccaaaaa gtaacctttt attttcagtt ttccaaagaa agtattttgg
     6121 ttcgatactt ggcatcaatt tgatcaattc agtcatcaac tggctactca acagtttaat
     6181 ctttctgccc atgtttttaa tcagcggagt gagtacttat atgcagctgc ttttcagttc
     6241 aaatgcggta cctacgggtg tcatgccaga agattttctt gatatcagct ttgttttatc
     6301 gatgataggg atgatttttg tttcagcact catcatgagt attgttggcg gtttattcca
     6361 atttgctgca tggacaaagt tcgactatcc tgatttgtcg gttattcaat gtttgaaata
     6421 tggctggttt ttattgaaag atagaattgg tacgtatatt ttgttgcagc tgtcttttat
     6481 cggctggtat ttgctaggag caatcgcttt gttcatcggt cttttgtggg tcattgttta
     6541 tgtgaatgtg tcagttgcag aattttatga acaagcacgg attgaaaaag gaggtccaga
     6601 cgcctatttt acttagcatg taagcagttt attcatcaaa aaaataactt ttgtggtagg
     6661 ataaatcttt gagggggaat gtacgatgaa attaactgtt ttaggatgct taggagcgta
     6721 tccttataaa aatcaaggga caacaggtta tttgcttcaa tctgacgggt tcaatttgtt
     6781 gatcgatgca ggtagtgcta cgctgatcaa attacaagaa caccttgatc cacttgatct
     6841 agatgctgtg atccttagcc attatcatca tgatcatatt gctgaccttg gtgtattaca
     6901 gtattactgg caacttcatc ctaagagaaa tccggagaca ttgctttcaa tttatggaca
     6961 cacaaaagat tccattcact ttgaagaatt gacgttggaa aatgtctcaa aaggacatcc
     7021 ttattttgaa gctgaagaat taaaaattgg tccttttctg attactttta tggaaacaat
     7081 ccatccagta gtatgttacg ccatgcgtat cgtagaagaa acaacgggga aagtattcgt
     7141 atttacaggt gattcaggtt atttggaaag tttcgctgat tttgcgaaag atgctgattt
     7201 gtttctagca gatacctatc tttttgaagg aaatgaacat catcatgccc atttcacttc
     7261 aaaagaagct ggagaacttg cgaaatcagc taacgtaaag aaacttgtac ttacacacct
     7321 acctcagttt ggtgatttag aacaattgaa aaatgaagcg caaaaggcag caggcaaagg
     7381 gattgatata gaacttgctg cagttgacaa agtgtttaac atttaggagg aagaacatga
     7441 tttttgtacc aaatgaaaac cacgacccaa gaatcaattt agcaattgaa caatatttgc
     7501 tgcaagaaat gcctttagat gaacctattt tgctctttta tatcaatgaa ccttcaatca
     7561 tcatcggcag aaaccaaaat acgcttgaag aaatcaatcg agattatgtg gaagaaaatg
     7621 gcattcatgt tgtccgtcgc ttaagtggag gcggagctgt ctaccatgat tttggaaacc
     7681 tgaactttag ttttatcatg cctgatgatg gggattcatt ccgtgacttt gcgaaagtaa
     7741 cacagccaat catccaagca cttcatgaat tgggtgttgc tggggcggaa ttgaaaggcc
     7801 gaaatgatct agtcattgat ggcatgaaat tttctggcaa cgcgatgtat gctaccaacg
     7861 gtcggatgtt tgctcatgga acaattatgt tcgactctga tatcaatgaa gtagtcaatg
     7921 cattaaaagt aaaaaaagac aaaatcgaat caaaaggaat caaatcgatc cgttctcgtg
     7981 taacgaatat caaaccattt cttccagaag aaaagcaaaa aatgacaaca gaagaatttc
     8041 gtcaagaaat cttgttgaaa attttcggcg tagagtcagt agaagaagta aaaacgtacg
     8101 aattgactga tgaagattgg aaaaaaatca atcgaatctc tgaggaatat tatcgaaatt
     8161 gggattggaa ttacgggaag tctccagatt tcaatttctc tcgccaaaaa cgtttttcta
     8221 tcggttcgat tgaagtccat ttgaatgtct cagaaggaat gatcaaagaa gcaaaaatct
     8281 ttggcgattt cttcggacta ggagatatct ctgacgtgga aaaaaaattg gttggacaga
     8341 aatatgataa agcatctcta tcaaaaatag tcagccaaat tgatgtgaag aaatattttg
     8401 gtgcgataga accagaagaa ttgcttacct tactttattg aaaataaaaa atagcggaat
     8461 ccacatatat ggattccgct attttttata caggattcgg gtaatttcga gagagttgaa
     8521 cagtgatccg atcaatgaaa gtatcttttt cctgcgttag tgttcctgtg taacgatccg
     8581 ctacgtcttt catcatcatt aagtcaaaac gttcctgatc atctaattct tctgtgatgc
     8641 ctcgactaaa taggtttgta tcgtaatgtg cactatgttc gcaacaaatg acgaaatgtt
     8701 cctcttgttc gtgtatcgac aaataaattt ttcgatacag tggattttcg atggcatgag
     8761 tttctctaaa ggaatgctct aaagcgtgga tcaaaagttg agccagatcg tcatcgttga
     8821 taggaagttc ttcgggaaga tctgcttgga aggttatctg aatattccgt ttggcagcta
     8881 acttctggta acgcgctaag ataagatttg tcacctggtg gttgcaaaaa gaacacagca
     8941 tttcgaaatt ttttgcttct tctaatatct tttccacata gtttttagct tttgtttggt
     9001 ccgtatgcat taaatcttgc agattctcca tctcttggac aaattccagg cgaagtgtat
     9061 ttacttcttg gatatgctgt cgaagctgct cataatgttg ctccaacaaa tcatttttga
     9121 tttttaagaa atctacttgt ctccgatctt tgtcggtatt taagatgtga tcgattagat
     9181 tgtagctgat aataacgatt agaatgataa tatataaaat cgtagaccaa ttgatcgttg
     9241 tttgatagat acctaatgcc gtttggatgt aaatcaagca atggataatg gctaccaaga
     9301 cgatccaagg tgtacaagct acataaaaac ggttattctt atatgcttcg ccaatgcatg
     9361 caagtgaagt gactaaagtg cttataacac tagcaatatt agcaatcggc attaattcag
     9421 gaagttctgc aggagtaaaa gttgcataaa gcagaacgcc gataaaaatc aaaagttgga
     9481 acgtacaaaa aattccgtac cattttttat agtaaaacat tcggctccaa tagtagcaga
     9541 tgatgaaagc tgacgttaag ataataaata aatgagataa aaaagaatga acgaatggtt
     9601 caaaaagaat accacttaaa atatcttctg atacagcttc aaatcctaat gccaaagcaa
     9661 agcaacttaa gagaataagt gagtattcaa gacgtttttg tttatacatc aagtagatcg
     9721 tatagaaaaa ggtccctata gcgataaaaa cagcgattat catcgtcaat acttgcggca
     9781 ctgattgtga ataaataaaa ctaatcaatg gtccagttgt tcctagataa actttcggtg
     9841 gcagtccagc atagttctga taaggagatg agactgtaag ggtcaatttt tttccgatat
     9901 aatttgctgg taaatctatc acagccaatg agagtccggg attgctttct tcctgataat
     9961 ctttccgttg gtataaaatc ttttcgccta gatatacagt aatccattga tggtttcctt
    10021 tgattagtaa atttgcttcg tccaacggtt tttcaagtgt cgtttccata atgaatgttt
    10081 cgtctttatc cacggtagga atcgtacgag tgtacttgct gtcaaactta aaatcaggat
    10141 catcttttgt atagaaaacc cacgaatctt ttatttcttt catttgattg ctgtccgcag
    10201 gagaaaaaat aaaataaaag aaataagcaa ttcctgttaa aaataacaat gataatataa
    10261 tgattatgct ccactttata cgtaaataaa aagattcttt tttattatat atgttattaa
    10321 aaaaagacat aaaggtattc ctttctaaaa cttgtaatgt tagggtaaac gttttttaaa
    10381 ctaacgtcaa ttatttataa taaaaaatga gataaagatg aggaaataga attagaaaca
    10441 tataattatt aaagggaaac atttttacag ctataatttg aaaaaattta tcgttattat
    10501 atgattaaga aggattgaat aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat
    10561 aacttttata agttctataa cgttgattat atttttaatt cttattcttc tcgtcgtctt
    10621 ggttccccga atttttggtt tgactcctta tattattgag gacaaagaaa tgagtccata
    10681 ttatgcacaa ggaaatctga tatatgtacg accgaaaaat acaaatgaga ttttagtcgg
    10741 agatattatt acatattatg aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc
    10801 cgttgaaaac aaccacgaag atttttatac aaaagctgat acaaagactt atattgagcc
    10861 tggagtagta aaaaagagaa atattatcgg tagtcccatc tttcagattc catatattgg
    10921 actagtcctt tcaaaaacgg catttgcatg gattcagggg ttattcttta cgattgcttt
    10981 ttgcctaaca ggaatcacag catggaatac ctttgtagag ttgaaaaaga agcggagacg
    11041 aacacagaaa tttttgtaag aatgttaaaa acataaatgt attccagaga tccaacgact
    11101 cttctgaaca tgaaacacga gaaaacactc atgcagcttg tagatttagt cagttgttcg
    11161 tataatgtgt gaagaggagg agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca
    11221 aagaagaact attggatttt atgcgtaccc agcaaaaaaa attaccgggt gagctaggca
    11281 aactggaaga agaagctcat gtagcagaag taccagttat tccccatgaa acagttgtat
    11341 ttttgaaatt tttacttgga cagcttcagc ctgaacgaat cttggagatc ggagcagcta
    11401 tcggtttttc atcaagtgtc atggccacga caatcggaga aaacgctcat gttacgacaa
    11461 ttgatcgttt cgatgtcatg atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga
    11521 cagataaagt gactttgctt gaaggtcaag cagcagatat cttgccggag ttgtctggtc
    11581 cgtatgactt tatttttatg gatagtgcaa aatcaaaata tattgaattt ttacctgaat
    11641 gcttacgttt gttaagaaaa ggtggcgttt tgatggtaga tgacatcttc caaggaggaa
    11701 cgatcttgct cccagatgaa gagattcccc gtggcaaacg agctatccat cgtaaattaa
    11761 atgaatttct tcgtgttgtg atggatcatc ccgatttgac atcgacaatc ttgccattag
    11821 gtgatggtgt gatcttaatg acaaaggaat cagaaacaat cgagttgtga tacatgatta
    11881 tggcagcctg tagataatcg attgaatcga gggctcaatt cgaacttgat gtaaaagttt
    11941 ctatatttgt aagtcaatta aaggatacag gaaatctaaa taaacaggag tagcatgaag
    12001 tcaatgtagc tcctgttttc aattgataac cttagaacag ctgaaggaga atgtctcgtt
    12061 ttaaaagatt gatttagtag ttgggccaaa ataattcatt atttgctaat taaataacta
    12121 tttcgtattg acagaagcaa tttaattatc tataattatt gatgttccgc tgattcttgt
    12181 catagtagct cagctggata gagcatccgc cttctaagcg gacggtcggg ggttcgaatc
    12241 cctcctgtga cgctaaaaaa ccgttagaaa ctgaggtttc taacggtttt tctttgtttt
    12301 ttgtgccaat attgtttgtt ttcaaaggca aataaaataa aaaaacatcc ttatcgagta
    12361 caattataag gagcaaaaaa ataaaactca tcagaaaaga ggaagaagat atgacgatac
    12421 tatctagaat tttagtgact ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa
    12481 cggtgagaac tgattctgac acgacaagta gggtttttaa aatgagtaaa gaagagttaa
    12541 gcagaaaatc tgttcaaaca ttgtttaaga accaaggagt gtacaatgga ctattaggag
    12601 tgggactact ttatggagct tatctttctt ccgcatctaa agaaatcaca agtatgctat
    12661 taatatctat tttttttgtt gcattatatg gcagtttgac tagcgatcgt tggattttac
    12721 tcaaacaagg tgggctagct ctgttggcat tgctttctat tttcttataa atatagaaaa
    12781 aataagaatt tatctaacag gaaactttgt gaaaaatatt actttatctc atgaaatatg
    12841 atattctaat gaatggtatt ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa
    12901 aaattatgtt tcctcactaa aaaaagaatt ttcaggttat gatctttcaa gatttcagaa
    12961 agatcttttg gctgggatca cagtagctgc tgtagcattg cctttagcct tggcttttgg
    13021 ggtatcaagc ggagctgatg cggccgcagg tttgattacg gctatcattg ccggtatcgt
    13081 tattggcggt ttatcaggcg gtttttatca aatatcaggt ccgacaggtg cgatggcagc
    13141 tatcttgatg tctattgcgg caacaaaggg aatgcaaggt gtattgcttg ctactttttt
    13201 agcgggtcta tttttacttg cagcaggact tttccgatta ggaacactga cttcttttat
    13261 cccagcaccc gtaattacgg ggtttacttc aggtattgct atcattattg cattaggtca
    13321 gatcgataat ttatttggtg ttcactcgga aggtacaaac gttattgaaa aactaagtag
    13381 ttatcaaacg ataggatttc cgataaacac agcctctttg atcatgggag ggctagtcat
    13441 tttagggatg tttatctatc caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc
    13501 tatcattttg gcaacagcag ttatgctatt gttccattta ccagtagcaa ctgtgggtaa
    13561 gattccacaa acattgatca gtagtaatcg tttgaatatg ggagatttta gcttttctgc
    13621 cttgcaacaa gtggcagttc cagctattag cattgcttta ttaggaatga ttgaaagctt
    13681 gctatgtggt gcttcggctg gccgaatggc aaataagccc ttagatagca atcaagaatt
    13741 aatggcgcaa ggaattggaa atctgttgct tcctttcttt ggcgggattc ctgctactgc
    13801 ggccatcgcc cgtacaagcg tggccatcaa atctggtgca caaactagat tagctggtat
    13861 gatacatgca gttgtcttac tgctctccat gcttgttttt gcgccagtga tgtcacagtt
    13921 tccaatgcca gcacttgcag gtgttttgat tgtgacggct tggcgtatga atgaatggga
    13981 aacgatcaag gagctattta ctaataaata ttggtcagca gttctattgt ttatccttac
    14041 gatgatttgt acagttatct ttgatttaag tattgctatc gtgatcgggg tgatcggcgg
    14101 ctgtgtattt tttgttgtca aaagcgctgc tatcacgatt tcattagaaa cgatcgattg
    14161 gcaaagaatg gggctgccag aaacggaaaa gctgaataac tggacagtag tgtatattag
    14221 tggtccactg ttttttatgt cttcagaaaa actcaaaaat aaattggaag aactaaatca
    14281 tcacgatggt gtcattttct ccatgcgagg cgtaccaagt attgatttga cggcacgtgc
    14341 agtttttgat gaatttcaag aaaaagcaga gcaaagagga caaacgatta tctatacttc
    14401 cttgcagcca gaagtagaaa aacaactttc tcacttgtgg gaacatacat tcacgcatca
    14461 acatcaaaca gttgctcatg cgttgacagc tttacatgcg aaatatactg caattagcca
    14521 ttaaaacaat gaaaaagaac agacaagact gtaaactgaa gattttatgg tcttgtctgt
    14581 tctttttaat attgctgtaa aagtgaagct agtacttgcc agtcttgatg gataacaggt
    14641 acaagggagc gattgtagaa aatagctgct ggatgaaaaa gtggaagaat tcgatagttt
    14701 ttttctgacc attcgtaggc atccttttgt tctgttaatt gtaagacagg ccctgtatag
    14761 agttgaccat gatttttggt aaccgtatat tctttgccaa gtaaacgttg aagaccaatg
    14821 tttccaagtg tcgcaattaa ttttggctgg accttcatca gttcgtaatc caatattggt
    14881 gcatgcgcaa atacttcgga acgagtaggt gtccgatttg gataaacagt ttctgactgt
    14941 tgtgtccgtt tattgatccg atgagtcacg cgatacgggc ggctccttac agcactggtg
    15001 atatacacct cttctcgtgt taagccagtt gaagaaagtg cctgcatcaa ttcttttcca
    15061 gcttgaccgc taaaaggaac atgcgtatca atctctgttt tccctggggc ttctcccacg
    15121 agcatcagtt taggatgaag aggtccttgc ccagcgataa atccttctac aggacggccg
    15181 ttcattctta aagaagcttc ttgtatcagt tcttttgggt attccattta tgctcctcct
    15241 ttttctttta ttataagaat aaaagaaaca caatgcgact attttatttg gtagaactgt
    15301 gaggatagaa aagcagaagt attgcttgaa tttttgtttt actttttgat tttaaattta
    15361 gttgactaac ggttgctgtt ctggtaagct agtactgttg taattgtgga ctccacagct
    15421 acaaccgctc agaacaagta ataagcacag catatgctgt cacttcggat ggcgagtcta
    15481 agtatatcag aaggaggtgc tttatagcat gtacgcaatt attaaaactg gtggtaaaca
    15541 agtaaaagtt gaagtgggac aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga
    15601 aaaagttgtt tttgacgaag ttatcttagt aggcggagaa tctacaaaag taggcgctcc
    15661 aactgttgca ggtgcaactg ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt
    15721 cgttactttc aaatacaaac ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc
    15781 atatacaaaa gtggttatcg acgcgatcaa cgcttaatag cgtttgagaa agaaggccta
    15841 tatatgatca aaggaacatt taagcgaaat gacgcaggtc agatcgtttc attcactttg
    15901 accgggcatg ctgatgctgg tccttatgga agtgatatcg tttgtgcggg tgtttctgcg
    15961 ttagcgatca gcactgtcaa cgggattgcc tcattagccg gttttgagcc gattgttgaa
    16021 atgaatgaag aagaaggcgg ttatctttat accgaagtca cttcaggaat gacacaagaa
    16081 caaaacaata ttgctgagat tctattagaa aatcttttat taggattaca atcgatagaa
    16141 gctgaaaact cagaatatat ccaaattaaa acaattaatg ataaatagga ggtgcagacc
    16201 atgttgttat ctatgaatct acaattattt gcccacaaaa aaggtggcgg ttctacatct
    16261 aacggccgtg actcagaatc aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt
    16321 acaggtggat caattcttta ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg
    16381 ggtatcggtg gagacgacac tttatttgca aaagttgatg gcgtagtacg tttcgaacgt
    16441 aaaggccgta acaaaaaaca agtgtctgtt tatccagtag ctcaagaagc ttaattgaat
    16501 cttatgatta aagaggttgt gacaagactt ttgtcacagc cttttttgtt tataaaaaaa
    16561 aaagttgtag tggaggctac gaagtttact ttgtcttttt acacttaata tttttttgcc
    16621 aatttggaat caggatgttt tgataactat cgttttcaat tgatggaagc tatattgttt
    16681 gctatcagtg aaataattat atggtaaaca gtgttctttt tcttgtttcc attataaaaa
    16741 tcgtatcggg atttttcgtt cttttaaatt gaacatttct atctatccgc gtgttatcaa
    16801 caaaacaaaa tcaggtaaat agttgacgaa acagaaataa atacatagta ttatgttttg
    16861 aagcctaagt acaatagtct atagaaaagg agcgatacca ttggcaaaac gcattgaccg
    16921 aatttatatg tatgtcaaag aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg
    16981 agtaacaaca caggaaattg ctgaagtgtt agggattcag cgaacaaata gcagtaaaga
    17041 tctgaatcag ctagtgcgcg aaggaaaact gataaagaca gacggacgac cagtacgtta
    17101 catatatcaa acactcgtta cgcacgtaaa gccagttaca aaacatgtag tcagttacaa
    17161 agaaacgccg gtctctgaag aaaaaaatac actccaaata gatactaagg atatctttgc
    17221 aaaaatcatt ggagctaacg gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat
    17281 tttgtatccg ccaagaggat taaattgttt gattacagga cctaccggat caggaaagac
    17341 ttattttgca cacgcgatgt atcattttgc aaaagcgcat catgtaatag atgaggaaac
    17401 ggaactgatt gttttcaatt gtgctgatta tgccaataac cctgagcttt tgatgagtca
    17461 tttatttggg tacgcaaaag gtgcttttac aggcgcagat gaagaaaaaa tggggattat
    17521 cgatcaagcg gatggcggta tgctgttttt ggatgagatt caccggttgc cgcctgaagg
    17581 tcaagaaatg atcttttact ttatggatca tggaacttac agtcgtcttg gggaaacaac
    17641 gaaatcccat gaagcaaatg tacggatcgt aggtgcgacc acagaagacc caggttcttc
    17701 tctattagaa acttttgttc ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa
    17761 acgtcctgca aatgagaaag tcgatttagt aaaaatcatg attgcccacg aagcaaaccg
    17821 cacgcagcga aagatttctt tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac
    17881 ctatggaaat ataggacaat tgaagtcaaa tatccagtta gtatgtgcac gaggatttct
    17941 aaatcatatg caatcacccg aaatctcgat cacaatcgat gatttgacag aagggattcg
    18001 tagcggattg attcaattag caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt
    18061 ggagccaaag ataactgtag caccaaatga tacgatcatg aaaatccaat cggactccta
    18121 tgagttacct tataatctat atgatatcat cggagataaa gcagctttac tgaagtcgga
    18181 tggtttggat caagaagcaa tcaatcattt catttctaca gatatcaaca tccatctgaa
    18241 gtctttctat aaagaccatg gtttttcttt taatgcagac agcaaattag cagaatttgt
    18301 agatccgaaa atcatagaag tgaccaatca gatttatgta atggtcaaaa gatctttacc
    18361 atatgagttt cagcaaaact ttatttatgc tatgagcttg catatcagtt cttttttgaa
    18421 aaaaatcaac ttaggagaag aacggcatac gaatgataac atccgtgaga tggcttttga
    18481 ttttccagat gaatatgctg ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca
    18541 agtggagatt ccagaaagtg aagactatta cttgaccgtt ctactcgtct cgttacgggc
    18601 aaatcaagca agcggcagaa tcggtgtagt cgttgcagct cacggaaaca gcacagcaag
    18661 cagtatggtc caagttgtcc aacagttgtt agatgcagag aacgtacgag cagtagacat
    18721 gcctttggat atggatccga aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt
    18781 agatgaagga agcggggtca tactcttagt agatatgggg tcccttgcgt cgtttaattc
    18841 gcaaattcaa agagacactg gaattccagt acgtactgtt gatatggtaa caacttctct
    18901 agttttggaa actgtacgaa aggtctcagt attagggaca gatctagata tgttgcatga
    18961 atcattgaaa aattttcgag gttacgcaga aatatcttct gtagaatctg ttcaggaagc
    19021 aaaacctatt cataagaaaa aagctgtttt ggctatctgc gcatctggcg aaggtacagc
    19081 acaaagaatc aaagaattga tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga
    19141 agttctagct ttgtcgattg tggaagtgaa agaagaactg ccagttatcc aaaaagaata
    19201 tcagatcata gctactactg ggattactga tttgaatatc caagccccat ttattccttt
    19261 agaacgtttt attgatcaaa atatcgaagt cattttggat cagttgctga tggaatcaga
    19321 attggaggaa actgaattca tctccctaga tgaagagtca gctaaaaata cgtgtgttga
    19381 gtttatctct gataacttca tctttattaa tggtagtaag ttaatcgatc caatgtggca
    19441 attttctaca caaatctctc agacgacggg aatcggcgat gaagaatatg gattcaaaat
    19501 caacctagtg atgcacacag caggaatgat cgaacgtatt attcggaatg aaccattgac
    19561 tgtggaagaa aacgaattga caaatacaac gaatgatcct ctttatagcc aattggcagc
    19621 ctcagttgtt ctgttggaag atcagataaa agtaaaagtg ccgatagaag aaatgtatta
    19681 tctgctacgt ctagtccata atcagttaga taaaaaagaa tacactgtgc catgaataca
    19741 caaaaacgat acacttttat agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat
    19801 tcaattagtg tatcgtaaaa aagttggcac gctttttgct tatagtagtg tgtgctagga
    19861 ggtggttgtt cacacagaaa tgataggggt ttctgtgaag tagtgaggaa gaaggagttt
    19921 tgcagatgga tattcgattg gcacgtatag atgatcgttt aatccacggg caagtcgcaa
    19981 cagtttggtc gaagcaaaca ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc
    20041 aagataaatt gagaaagttt ctactaaaag aagctgcacc gccggggatc acagcaaatg
    20101 taataaccgt gcagaaaatg attcaaatct atcatagtgg attattatca cgagcaaaag
    20161 tgatgctttt attcaccaat ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt
    20221 tgaagtcgtt gaatatcggc ggtatgagtt ttactgaagg caaatcaatg attaccaatt
    20281 ttgtttcagt aaatcaacaa gatattgatt cctttcgtta cttgaacagt caagaagtgg
    20341 agttagagat tcgaaaagta cctgctgaca ggaaaatcct tttgatggaa ctactagcga
    20401 aaatcaaaaa ggtgtagcaa gaatggttgt tggaaaaggt caaataattc tgttgtaatg
    20461 gttagcaatt attaatattt ttgaatttgt tacaacaaaa gtaaatagga ggtataaata
    20521 tggtagggat tatcctagca agtcatggac aatttgctga aggaatctta caatccggtt
    20581 caatgatctt tggcgaacaa gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc
    20641 cagatgactt gagagccaaa ttggaagaag cggttgcttc ttttgataat caagacgaag
    20701 tgttattctt agttgattta tggggcggaa caccgttcaa tcaatcaaac acattattcg
    20761 aagagcacaa agataaatgg gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag
    20821 cctacgcatc acgcttttca atggaatctg ctcatgaaat tgcagcacac atcattgaaa
    20881 cagcaaaaga cggtgtaaaa gtaaaaccgg aagaattaga accggcagaa gcaccgaaag
    20941 cagcagttga agacgcacaa cctaaaggag cacttccaga aggaactgtg gtcggagacg
    21001 gaaaaatcaa atatgtatta gcacgtgttg actcacgtct gttacatgga caagttgcga
    21061 cagcttggac aaaagcagtg caaccaaacc gtattatcgt tgtttcagat gcagtatcaa
    21121 aggatgatct gcgtaagaga ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg
    21181 ttattcctat tagcaaaatg atcgaagtag caaaagaccc tcgttttgga aataccaaag
    21241 cattgctttt atttgaaaat cctgaagacg tattgacagc tgttgaaggt ggcgtagaca
    21301 tcaaagaatt aaatgttggg tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag
    21361 tattgtctat gggacaagaa gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga
    21421 aattcgatgt acgtaaagta cctaatgatt cacgcgataa catggatgat attttgaaaa
    21481 aagcaaaagc tgaattagca aaagcgtaat aaataatatt aaaatttata ggaggcttat
    21541 catgtctatt atttcaataa ttttagtcgt attagttgcc tttctagctg gtatggaagg
    21601 tattcttgat gaatttcaat ttcatcaacc actagtggca tgtacattga tcggtttagt
    21661 taccggtaac ctcgaagctg gtatcgtact tggcggtacg cttcagatga tcgctttagg
    21721 ttgggcaaat atcggtgccg ctgtagcacc agatgctgca ttagcatcag tagcatcagc
    21781 aattatttta gttttaggtg gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc
    21841 cgttcctttg gctgttgcag ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc
    21901 aatcgttcat ttgatggatg cggctgctga agaaggaaac atccgtaagg ttgaaatgtg
    21961 gcacatcatt gctgtatgtt tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct
    22021 atttatccca gcacaaactg tgcaatcatt ccttgaatca atgccagcat ggttaacaga
    22081 tggtatggct atcggtgggg gaatggtcgt agctgtaggt tacgctttgg taatcaacat
    22141 gatggctaca aaagaagtat ggccattctt cgttattggt ttcgttgtag ctgcaatttc
    22201 tcaattaaca ttgattgctc taggtgcttt aggtgttgca ttagctctta tttacttgaa
    22261 cctttctaaa atgggtggtt catcaaataa tggaggcgga agcaactctg gtgacccttt
    22321 aggcgacatt ttgaatgact attaatcttg aaggaggaaa ataaaatggc agaagaaaaa
    22381 atcaaattat caaaaaaaga tcgtttagct gttgcgtggc gctctacctt cattcaaggt
    22441 tcatggaact acgaacgtat gcaaaacggt ggctgggcat tttctatgat tccagcaatc
    22501 aaaaaattat acaaaacaaa agaagaacgt tctgcagcat taaaacgtca cttggaattc
    22561 tttaatacac acccatacat tgcttctcca attttagggg ttacattggc tctggaagaa
    22621 gaacgtgcga atggcgcgcc tgttgatgat gtagcgattc aaggggttaa agtaggtatg
    22681 atgggtcctt tagctggtgt tggtgaccca gtgttctggt ttacagtacg tccaatgcta
    22741 ggtgcactag gtgcttcatt agctatggga ggaaatatcc ttggaccaat catcttcttc
    22801 ttaggttgga acattattcg ttgggcattc atgtggtata cacaagaatt tggctacaaa
    22861 gctggttcta aaattactga tgacctttct ggcggattgt tacaagacgt aacaaaaggt
    22921 gcttcaattt tagggatgtt cgtattagct gcccttgtac aaagatgggt atcaatcaaa
    22981 ttcttaccag ttgtatcaac tgtaaaatta gataaaggtg cttacatcga atgggataaa
    23041 ttgccttcag gtggtgaagg aatcaagagt gcatttgaac aagttaataa tggcttagcc
    23101 ctttctcctg aaaaggtaac aacattacaa gacaacttag accaattgat tcctggttta
    23161 gctgcattag gattaacatt gttatgtatg tggttgctta agaaaaaggt ttctccaatc
    23221 gttatcatcc ttggtttatt cgtagtcggt gttgttggac acgtcatcgg acttttataa
    23281 tccataaaaa tatagctcgg gcgttgtccc gagctttttt tatatagtat actgttcata
    23341 aacaaatatc tggagggaga aaatggttca atcaatcaat acaaaagtag acttggtaat
    23401 tgatgctact tcgtttacgg gtctgacaga ttacgggcaa atcatgatag gtaataatgg
    23461 ttttgagttt taccatgcgc gagatagacg caaatatatc caaattccat gggaagaagt
    23521 ggattatgta ctagcatcag tcatgctaaa gggaaaatgg attcctcgat atgccattca
    23581 aacgaaaaaa aatggtacat acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc
    23641 gatcagagaa tatgtagatg aagatagaat ggtacaatca ttgagtttct ttgatgtagt
    23701 gaaacgtggt attcgtagta tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag
    23761 gaggttttct ttattttttt actttgcaga agaaaatatg aaatagcaaa accaattatt
    23821 tgtccccttt aaatcccata tgctaaaatt gattagcaaa gaaggaggtt atattatgag
    23881 ttggttatgg acattgattg ttggagccgt gatcggagca attgcaggtg cgatcaccag
    23941 taaaggaaat tcgatgggct gtgtaacgaa tatcattgct ggactagtcg gttcaagtat
    24001 tggacaagcg attttcggcg aatggggacc aaaagctgca ggtatggcac ttattccatc
    24061 tattttaggc gcagtgattt tggtagcagt tgtctcattt ttctttggaa aacgatcata
    24121 agtttttaca aaatagtaaa ctaaaaggag ttgcagcgag actgtgactc ctttttggct
    24181 ttatttattt aaaataaaaa gaagttttcc ggaaattttt atttaactgt cgtgtacgaa
    24241 gaaaagtttg ctataatgga aaaaagcgtg caaagaaaga gggagtttca ttatgatgct
    24301 acgtgtagaa aaattgcgca aaaaaatgca agaagaaaat ttggattcat ttttggttac
    24361 cagtccgtac aatcttcgtt acttaacgaa ctttacaggt acgacaggat tagctgttat
    24421 tactttggaa aaagcatttt ttattactga ttttcgttat accgagcagg cggcagcgca
    24481 ggctcaaggg tttgaaatta ttaaaaatgt cggacctatt tttgatgagg tagcaaactt
    24541 agtacgcaaa gaagaactgc actcattagc ttttgaggaa acaacagtct catttttaga
    24601 atattcggtt ttagaagaaa tcatcgatgc ggaattagta cctgtttcac agatgatcga
    24661 agaactcaga gaagtgaaag atgaagaaga aatcgctatt atcgaaaaag catgtcatat
    24721 tgcggatatg gcatatgatc acatcctgaa aatgatccaa ccgggcatga ctgagattga
    24781 agtcgcaaat caactagact tttatatgcg ttctcttggc gcttcaggtg tttcttttga
    24841 tacgatcgtt gctagcggat tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat
    24901 catcgaacaa ggtgacttga tcacattgga ctttggttgc tattacgaag ggtacgtttc
    24961 cgatatgaca cgtacattcg ctattggtga tcctggggaa aaattaaaag aaatttatca
    25021 aatagtctta gatgctcaat tagcagttat tgatgctgca aaaccaggaa tgactggtgt
    25081 ccaattagat gcagttgcta gagattatat ttcaaaacat ggttacgggg aagcatttgg
    25141 acattctaca ggacatggga tcggtttaga gatccacgaa ggaccaaacg tttcttctcg
    25201 tgcagaaaag caatttgtac caggaaatgt gatcactgat gaaccaggga tctacttacc
    25261 aggaatcggt ggagtacgta ttgaggatga cttgttgatc acagaagatg gaaatcgtgt
    25321 gttgactcgt tcaccaaaag aattgatcat cttatagttt catcaataga agaagtaagc
    25381 gtaaaaatca gtgttcacag actttgttat agaaaatcac tcgatttaat gatacactat
    25441 agaagaactt ttaagctgta agagataata aagttgggtg ttttctccaa ttcatagcag
    25501 tttgcagctg atcggcggca gttttcttac cacgtgatca ctatgccgca tcatagagaa
    25561 ttcggatgta tctcgtgctt aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat
    25621 ttgattttaa acgcaaacca agatttagga gagatcgtga tcgctcctga agtcatcgaa
    25681 gtaattatcg ggattgcagc ttctaaagta gaaggcgtat acggaatgcg cggaactttt
    25741 gccagcaatg tcactgaatt attaggacgt gcagctcacg gaaaaggtgt ttacttagca
    25801 aacgaggaag atggcttgaa agtagatctt tactgttatc tagaatatgg tgtttcagtt
    25861 cctaaagtag caatggaaat gcaggaacgg gtcagccagc aagtcctttt catgacggat
    25921 atcgatttat ctgaagtcaa tatccatgta gtagctgttg ttcctgaaaa attagaacaa
    25981 ccaacaatcg aagaactttt tgaagacgaa gaggaagaga atgaataaag aattatcaag
    26041 acacgagatt cgggaaatgg cattgcaggc tttatttcca ttagacttca atgcagacct
    26101 aacaaaagaa gatgcgattt tcaacgcaat cgaattagac caccgcgaca tgatcaacga
    26161 agatgaatca gagtttgttc ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa
    26221 aaaagatgaa ttggataaag tgattgaaaa acatttaaaa aacaattggc acatcaatcg
    26281 gatctcaaaa atggatttgg tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac
    26341 agatgtacca gcggctgttg ctttaaatga agcaatcgag ttggcaaaaa catttagtga
    26401 tgaccgttca cgtaaattcg tcaacggtgt tttatccaat gtattgaaag aattagaagc
    26461 tggggcgtaa gtcctagctt ttttctatga atcatcataa acctgttata aatcgggaaa
    26521 attctatggt agaatgaaaa cacaaaaaat atctgggagt ggttagatgg cagaattgat
    26581 taacggaaaa gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt
    26641 gaaagataat ggtatccatc ccggattagt cgttttattg gtaggggaaa atccagccag
    26701 ccaaatctat gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc attcattggt
    26761 agagcgttat cctgagacga tttctgaaga cgaactactt gcagaaattg aaaaatacaa
    26821 tcaagatcct agttttcatg gtatattggt ccaattacca ttgccaaagc atatagatga
    26881 agaaaagatc ctgttagcta tccgccctga aaaagacgta gacggttttc atccgatgaa
    26941 tttaggcaga ctccttgcgg gtaaaccgga caagattcct tgtactccat acggcatcat
    27001 gaaaatgttt gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg
    27061 aagcaatatc gtcggtaaac cgatggctca gctactcttg atggcagatg caactgttac
    27121 tgttgctcat tctaaaaccg aaaatctggc tgaactggca caagaagcag atattttagt
    27181 cgtagcaata ggacgtggac attttgtcac aaaagaattc gtaaaaccag gtgctgtagt
    27241 gatagatgta gggatgaatc gtgatgctaa cggtaagctg atcggtgatg tgaaatttga
    27301 tgaagtggag cctattgcag gctggatcac tcctgtgcca aagggtgtag ggccaatgac
    27361 tatcacgatg ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag caggtgaata
    27421 gatggcacaa gaatatttaa ctgtcactac attaacgaag tatttgaaaa gaaaatttga
    27481 tgccgatcct tatttagaac gcgtatattt aacaggcgaa atatcgaatt tccgtttacg
    27541 tgccaatgcc catcaatatt tcagcttaaa agatgaccat gcaaaaatat ctgctattat
    27601 gttcaaagga gcattccaaa aattacgatt ccagccaaaa gaaggaatga aagtccttgt
    27661 cgttgggcgt atttcattat atgaagcggg aggttcttat caaatctatg tagagcacat
    27721 ggagcctgat ggaataggtg ctttatatca agaattggaa gaacgaaaag aaaagctgat
    27781 gaaggaaggc ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc
    27841 ggtccttacc agtccaagcg gagcggttat ccgcgatatc atcacgacgg tgaaaagaag
    27901 atatccaatt gtccaattgg ttttgttccc aacagttgta caaggaaacc aagcagcgga
    27961 caatgttgtc aggaatatcc agagagtaga agagatgggc aatttcgata caatgatcat
    28021 cggtcgtggg ggtggttcga ttgaggattt atggccgttt aatgaagagc gggttgctcg
    28081 ggcgatttat caagctaata cgccggtgat ttcatctgta ggacatgaaa cagataccac
    28141 gatcgctgat ttagtagcag atgttcgggc tgccactcca acagcagctg cagaattagc
    28201 tgttcctgtt ttgtcagaag agttgatgcg cattcaagaa cggcaagcta gattgcagca
    28261 ggcgtttgta cgccagatcc agcgcaaaca agaacggttc gagcgtgctc gacgttccta
    28321 tgtatttcgg cagcctgaaa gattgtatga aggtcagacc atcaaattag accagttgaa
    28381 acagcgatta tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc
    28441 attaagccac caattggaac aagtggctcc gctttatcga gttaaagcag ctcgccaaga
    28501 aactgcttat ctagaaaaac gcttagaaga gaaaatccaa caatatatga aacaacagca
    28561 gcagcgtttt gctcaagctg ttcaatctct tgatttgttg agtccattga aaatcatggg
    28621 aagaggctac agctatacga cgttaaatga tcaagtagta aaaagtgtca ccgagattca
    28681 tcaaggggat ttgctgacga tccattatca agatggaatc gttaaaggac aagtacaaga
    28741 gatagacaaa ggagaataaa aatatggaaa atccaacttt tgaagagtca ctgcaagaat
    28801 tagaaaaaat tgtcatgcag ttagaacaag gagacgttcc attagaatca gctctagatt
    28861 cgtttaaacg aggaatggaa ttaagcaaac attgccaaga tacattagct aaagcagaag
    28921 agaccttgac gaaaatgatg acagaatcaa atgaagaagt tgattttgac ggaaacgagg
    28981 aaagctgaga tgagattaac tgacttttca gctattcatc tacctgctgt agaaaaagaa
    29041 atcttgtctt ttttagatga gcatacaacg gatagaggac tctatgatgc gatgagttat
    29101 tcagtgaaag caggcgggaa aagaatacgt cccttattac ttttaacggc cgttgcttcc
    29161 tttgatgagc ctattgatgt tcccgtctat caagtagcag ctgctttaga aatggtgcat
    29221 acgtattcct tgatccacga tgatttgcct gcgatggaca atgatgatct tcgtagagga
    29281 aaacctacca atcataaagt attcggcgaa gcattagcta tattggccgg agatggtttg
    29341 cttacaggag cattccagct aatcagtatg gcacacttag gtaattctcc taagcttctg
    29401 ctgcttcagc aattagctgt ttgtgcggga tcacaaggta tggttgctgg gcaagctgca
    29461 gatatcgagg gcgaatcgaa aaagctttct ttagaagaat tagcatttat tcatgaaaga
    29521 aagacagggc atttgattcg ttatgccttg ctggctggtg gcatattagc gaagcaacct
    29581 gaagaaatct tattgctact tcaacgatta gccgaacatt taggtttagc ttttcagata
    29641 agagatgatc tactcgatgt gattggtaca acgaaaacat tagggaaaac agctggtaaa
    29701 gatgaacgta tggaaaaaaa tacttacccg cgtttattag gcctagaaaa gacacgtgag
    29761 gcgttggaga ttgagctgtt atccgccaac aagatcatag acaaattaga agaggacgtt
    29821 ttttcttttg atggcgggct gatgcgccag atgatcaagc aatttgatgt ggagaaatga
    29881 gggaaagaat ggaaaaagaa cgcgtagatg tattagcggt caaacaagga ttatttgaaa
    29941 caagagaaca agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc
    30001 ggtttgataa accgggagaa aaaattccag tgagtagtga attaagagtc aaaggtaaaa
    30061 aacttcctta tgtttctcga gggggattaa aattagaaaa agcactcaaa gcatttgatc
    30121 tatctgttga aggaaaaaca ttgttagata ttggtgcatc cactggcgga tttactgatg
    30181 cagctttgca aaatggcgca aaaatgagtt atgctttaga cgttggctac aatcagctag
    30241 cttggaaaat aagacaagat cctagagtcg tcgtaatgga aagagtgaat tttcgttatg
    30301 caaaacccga agactttacc ttaggaattc cagaagttgc tgttatcgat gtttctttta
    30361 tttcactaaa actgatgctg cctccattac atgctatttt aaaagaagag ggagaagtga
    30421 ttgctttaat caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa aacggaattg
    30481 ttcgtgaccc tcagacccat caaaaagttt tagaagacat tttgtcattt gcggcacaag
    30541 gtggatatga tgtgctggaa ttaagttatt ctccgatcac ggggggagaa ggaaatatcg
    30601 agttcttggc tcacttgagg aaagtacccg aaagcggaac aatcaattca gctattaata
    30661 tggcagaagt ggtttcaaat gcacacgaac agtttgacca taaataaatt gtaactgtga
    30721 aaatcaagtg agaagtatgc ggattcttct ttattatacc aaggaaataa gctatcattg
    30781 attaagaaga atatgctaag gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg
    30841 attactcgat tgttgagtga gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg
    30901 aaaagcaaag gaatatctgt tactcaagct acgatttcca gagatataaa ggaactgaaa
    30961 ctgatcaagg taccggcttc agatggcggc taccgttata gcataccagc tgaaacaaac
    31021 gaagatgtaa atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa
    31081 atggaaaaat ttgttatttt gaaaacattg ccgggaaatg cttcggcagc agcaaatttg
    31141 attgataaac gttacaagaa agaattattt tcgattatca atgatgacga taatgttttg
    31201 atgattacac gaactgaaga ccaggcagtt ttattgaaga aagacttcct gcattacctt
    31261 taagttgagg agcgaatgtg atgctacaag aaatcagtat tactaacttt gcgattattc
    31321 cggaattgcg gctttctttt cacgaaggaa tgaccgcgct gaccggagag actggtgcgg
    31381 gtaaatccat tattatcgat gctttaggtc tgttagcagg aggaagaggt tcaagcgatt
    31441 atatacgtca aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag
    31501 aaggattctc tgaactgatg gttgaattag ggatcgaaac agatgaggac aatctgattg
    31561 ttcgacggga tatgtcctta actggaaaaa atgtttgtcg agtgaatggt catattatca
    31621 cattagctaa tctgagaaga atcggcagtt atttagtaga tatccaagga caaaacgaac
    31681 atcaggaact gctccagcct gaatcacatc ttgctttatt ggatcgtttt ggagatgtag
    31741 ctttccaaca gaagaaaaag agttatcagc aagaatatat ctcttatcgt gagctggaaa
    31801 aaagggtacg aaaagtgcag caaaatgaaa aatcctatgt tcagagaata gatatgctcc
    31861 atttccagca agaagaaata gctgctgctg atttgcaagt cggagaagaa gagaaattaa
    31921 aagaagaaag agaaaaactt agcaattatc aaaaaattgc tgatggatta gctgcaggtt
    31981 acggagcact gacgaataat gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag
    32041 aactgcaagg gatcgcccat ctagatgtgg agtatgaagc aatttatgaa aatatccaaa
    32101 gtgcttacta tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt gatttattgg
    32161 agttagatga gagtcgttta gaagaagtta cgcaacgttt agaattaatc cgacagttga
    32221 aacgtaaata cggcgaatcc attgagtcaa tattggctta ctatgacgaa atcaccgaag
    32281 agctagcttc ttctgatttt tcagaaggtc aattggacaa aatgaaagag gaactagagc
    32341 aaaaagagct tctacttcag caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg
    32401 caaaagaatt ggaaaaatcc attctgcatg aattgaaaag tctgtatatg gaaaacactg
    32461 agtttgaggt acgtttctta aaagaagaaa atagacaatt gaatcatgat ggatttgacc
    32521 aaatcgaatt ttacattacg accaatccag gagagccact gaagcctttg gtgaaagtcg
    32581 cttctggtgg cgaactgtct cggatgttgc ttgctttgaa aactattttt tcttctgagc
    32641 aaggtgtgac aagtattatt tttgatgaag tggatacagg tgtgagcgga agagttgcac
    32701 aggccatcgc agataaaatt tctcaaatat cgaaatactc tcaagttttg tgcatcactc
    32761 atctgcctca agttgctgca gtcgccgact atcaatatta tattgtgaaa gcagtgattg
    32821 atggacgtac gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg
    32881 cacgtatgct ggctggaagt gagattacaa aactcacact cgaacatgca aaagaactat
    32941 tgaaactcgc aaaaaggacc tgagccacct cgcagtggtt caggtccttt tcagcagctg
    33001 atagctatat tttattttta catgacactc attacagcag tcaatacaac agatccaatc
    33061 gttaaaatca gcattgtcca aatgacaaat tttgttactt tagcaaaagt acttgtttgt
    33121 tttttttcac gcattgtttt caacctcgtc attaattagt tgattcaagt gtacaatctt
    33181 ttttccattt ccacaagagg gcttttttat aaaaacttcc ctttttttaa ataataggta
    33241 gttacgatac aaataaataa tgttaataga actagccata taaaggcatc atccctttca
    33301 gcaataggta acttaatgtt cattccatag atactgccga cgattgtcgg tatagttaaa
    33361 ataatcgtta aagaagttaa aattttcata atattattca gattattcga tacgattgct
    33421 gaaaaagtct gattcatttg ttctactagt tttaattgaa tgcgagaagt agtcattgcc
    33481 tgctctgttt ccaccaatac atcacgtaaa tgatttttca aagcatgatt ttcttggaaa
    33541 agtttatttt gcgctagttc tttcaatgca tctaaattag cagaagtcgc ttctctgaaa
    33601 taaacgaggc ttttttggat atccatgatt tgataaagtt gtttgttttc agtggatact
    33661 tgaagtttgg cttccaaagt atcgcattga gattgaagcg tagctaaata actgttgtaa
    33721 gagagtgtga cctgccaaat gaattgaaag aagatattga gattaggcga aatatcactt
    33781 tttggaaagg tctggctaaa gactttcttt aaaaacagtg gttcatgatt cgtaattgtg
    33841 ataagcttct ttttgggcgt caaaataata gagagcgggt atgtatccac ttgaaaataa
    33901 ccgctaggac ttgttttagc aaaaggatat tgtaacaata ataacgctgg ctttgtcaac
    33961 tttgattgat tgaattcttc tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg
    34021 taatctgacg gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg attaggacgt
    34081 tctacacaat accagatcgt ttggtcagtt tcctttccgg tattacgcag tttctcatta
    34141 tctaatgtaa aatatttgat catgattttc ctcctgttgc ctgtttcttt tattataacg
    34201 gatagggaat tggaatacat ggaatgtgtt tgtaagatat agaatataac tgcataaatt
    34261 taaataaaat gaaacagttg tgtaaagtat atacagtata cttgaagcaa cgataaaaaa
    34321 ggagtttacg ttcatgggca cattcacacc acttatcttg ttttggtata tttacccggt
    34381 aatcgttttg tttggttgcc aatttcttgt aaaattattt tcgttaaatc gtcgttttaa
    34441 aataaaagcc cctgatttgg cgattccttt tttgtggggc ggtatgcatg cattgtctag
    34501 aaatacaggg acaatttcta tcttaccgtt tttctttatt tctgttttgc taatgggaat
    34561 ttgtattgct gttttccaag catattatta tgaagaaatt atttatccgc gttatttcaa
    34621 gatgacttgg cgtttggttt ttctgttgac aatcgtcttg tacgctgtct tgatcattgt
    34681 gaacatactt tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct
    34741 ttttgcagct tgatttttaa aaaaatgttt atgaataaaa aaagttgttc ccggatttcc
    34801 cagaaaaggt ggtagaaagt gggggattgt ggtagactaa ttttagttag tggaggaatg
    34861 gggggctttc aatgttgatg ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa
    34921 tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg
    34981 atggctgtct tttcggctat ccgatgagcg aatgggaaaa tctggaagct aaactgaacg
    35041 aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg attcttctat tcggctgcca
    35101 ctgaatgcga acttgataaa caaggacgaa tcaatatacc cagcacgtta agaaattatg
    35161 cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg
    35221 agacacgatg gcaagatttt tctgcagcag cagaagagaa ctttgacgaa attgctgaaa
    35281 gtatgattga tttcggacta taagaatagg aagaggctga ctaatgactg aaacatttca
    35341 acactataca gtcatgttga aagaaacggt agatggattg aacatcaaac cggatggcgt
    35401 ttatgtagac tgtacactcg gcggtgccgg acatagtgaa tatttattaa gccaactaag
    35461 ttctaaaggg catctgtatg cgtttgacca agatcaaaaa gcaatcgatc acgccaagct
    35521 gcgtttagct tcttatgtcg aacaaggtat ggtcacattt atcaaagcga acttcagaga
    35581 attagaagaa cgcttgagtg agcatgtaga taaggtagac ggcattttgt atgatctagg
    35641 ggtttcctct cctcagctag atgaagcaga acgtggattt agttatcatc aagatgcacc
    35701 gttggatatg cgcatggatc agtcagctcc tttatccgct tatcacgtaa ttaatgaata
    35761 ttcctaccat gaattagtaa agattttctt ccgctatggc gaggagaaat tttccaaaca
    35821 aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa ctggtgagct
    35881 tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc
    35941 aaaaagaatt tttcaagctg tgcggattgc agtaaatgac gaactcggtg ccgaagaggc
    36001 ctctttagaa caggcaatcc ggttattgaa agtcaatgga agaatcagcg taatcacatt
    36061 ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga
    36121 tctaccaccg ggacttcctg tcatacctga cgaattccaa ccagaactga aagtcattac
    36181 tcgtaaacca atccttcctt cagaggaaga gctaacagaa aacaatcgat cacgcagtgc
    36241 aaagttacgt attgcagaaa aaataagaat gaaagaggag tagaatgatg gcagaattaa
    36301 agaaaatgca agattacgaa tatgatttac ctgaattggt ggatcagccg gaacaacaaa
    36361 tgcctgattc ttcttcgaat caagaaatca ttcttccaca atcaccggca aaacgattaa
    36421 agcagatttc tgctttggaa aaaattgctg tggcaagcgt ggcgattgct attgttgctt
    36481 tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt
    36541 caatacaaac agaaatcaat gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa
    36601 acgaactttc tcgtactgaa cggattaaac aaatcgcaga agaaaaagga ttgtctatta
    36661 atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta
    36721 tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc attttatttg
    36781 ctacgagtat tggattgttc tttttatttg ccgtccgttt ctcgtatatc gttattggag
    36841 gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag
    36901 tagtaaaagc aaaaagagga acgatttatg atcgtaacgg tgtggcgctc gcagaagatg
    36961 cctcctctta ttcaattaaa gcgatactct caaaaacata tacctctggg gataaaaaac
    37021 tgtatgttga agaaaagaat tttgataaaa tagcagaaat cctgcataag aatctttcta
    37081 tagacaaaaa agatgcactg aatattttag aggacggcgc aaaaaaagag ctgtatcaag
    37141 ttgaatttgg tagctatggg aaaaatatct cccaagaaac caagcaaaac atcgaagcgg
    37201 acatgaaaaa agaaggtgtc gctggtcttt attttgtcga ccatcaggct cggatgtatc
    37261 caaatggcgt cttttcttct cattttattg gatacgcagt tccagacaaa gatgagaatg
    37321 gcttagttgg taaactaggt ttggagtctg cttacaatga tatcttgagc ggtaaagatg
    37381 gtaagattat ttatcaaaaa gataattttc aaaatcctct gccaggtaca gtggcagaag
    37441 aagaaaaagc tgtcgacgga caagatatct acacgacttt agacagcaga ctacaaagtt
    37501 atttagaaac actgatggat caagtaaatg aagaatatca gccagaagaa ttgacagcag
    37561 tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg acaacgacct actttcaatc
    37621 ctgaaacaat ggaaggattg actggaaaag atgctatctg gcgtaatttc ttggtacaag
    37681 atagttatga accaggatca accatgaaag ttttcactac tgcagctgct atcgaagaag
    37741 gagaattcaa cgagaatgaa accttccaat ctggaaaaat ccaagtagaa gatgctacga
    37801 tcaatgacca tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt
    37861 caagtaacgt cgggatggtc atactggaac agcgattagg cggacgatgg tataactatt
    37921 tacaaaaact gggatttgga caaagtactc attctggatt agatgatgaa gtaaacggtg
    37981 ctttacctac attaaatatt gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg
    38041 tgacgaattt ccaaatgatg aaagctttca cttctattgc gaataacggt acaatgattc
    38101 aacctcgata tatcagcaaa gtggtggatc cccaaactgg agaagagcga acaactcaaa
    38161 cagaagttct agggcaacct ttttcaaaag aaacaacaga aaaagtccgt gaatatatga
    38221 gagatgttgt cgaaagcgaa aactacggta gtgcttatgg tgtatatagt gtacctggat
    38281 acaatgtatc ggcaaaaaac ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa
    38341 caggagatac agcctacctg tattcgattg tggagatggt tccttctgaa gatccagact
    38401 atgtcttgta tctaacaatg aaacacccta agacatatga tcgaatggca ttggctaaaa
    38461 tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga aactgaagaa gattcagata
    38521 cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg taatcttgaa gcggacgttg
    38581 cagcagctga tgctcaaaaa agtggactgc aacctgtagt gatcggtaat ggtaaaaaag
    38641 tacaaaaaca atctactgcc aacggtgatc agttgatctc tggagaaaaa ctgatcctat
    38701 atactggtgg tgataagctg atgcctgacg taacaggatg gtcaaaagca gatatcatga
    38761 agctggggaa aatcctcggt attgaagtta gttttgatgg agatggttat tgtgtcaaac
    38821 aagaacttgc tccatatgaa aaaatcactg aagacaaact tagttttact ttagaagaat
    38881 aagaagagaa ataggagaga aaagagcatg gaatggacac aagcattgat tcccattgtc
    38941 agcagttgtg caatgacaat tgccgcaatg ccgctattta ttggttattt tcaaatgaaa
    39001 aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc acaatagcaa agccggaact
    39061 cctacaatgg gcggcttagt gtttctgatc ggctctattt tgacaggaat ttgggttgga
    39121 gcttggcaaa aacagctaac tcctactctt ttcatcttgc tttttgtatt agcgttatac
    39181 ggagtaatcg gctttcttga tgattttata aaaatattca agaaaagaaa catgggactg
    39241 aactcgaagc agaaattgat tggacagatt ctagggggaa tcatctttta tcttgtttac
    39301 cgttctgaag gttatccagg tgtattgaat ttcttcggac tagatctacc tttaggaatc
    39361 atttatggta ttttcgctct cttctggctt gttggatttt caaatgcagt aaatctgaca
    39421 gatggcatcg atggattagt tgctggctta ggaagtatct cttttgcagc ttacggtcta
    39481 attgcttggc atcagcatca gtatgatgta ttaatcattt gcttaagtgt tttaggaggt
    39541 ttactgggtt tcttcgtcta caacagaaaa cctgccaaga tttttatggg agatgtcggt
    39601 tcattagcgt tgggcggact tttagcggca atttctataa tgctaaacca agaatggaca
    39661 ttgcttttga ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct tcaagttact
    39721 tcctttaagc ttacaggaaa acgaattttt aaaatgtctc caattcatca tcactttgaa
    39781 atgtgcggat ggtcggagtg gaagattgat acagtttttt ggttgatcag cattgtgact
    39841 tcgttgatca cattatggat cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac
    39901 atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa
    39961 attattacat gaattgggcg cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa
    40021 tccggaagca caagagctgc tttctttagg gataaaagta ataacaggta gtcatccaat
    40081 cgaattgttg gatgaagaat tttcattgat ggtcaaaaat ccagggattc cctacagtca
    40141 tcctttagta gcaaaagccc aagaaatggg tattccggta attacggaag ttgagctagc
    40201 ttacgaagta gcggaatgcc caattattgg gattacagga acgaacggaa aaacaacgac
    40261 aacaacgatg acaggactac tactgaatgc aggagcagat caaggaattg cacgtttagc
    40321 gggtaatatt gggtatccag caagtggtgt agcgcaagaa gcaaaatcag aagataaaat
    40381 tgtgatggaa ttgtctagct tccaactcat gggaatcaca gatttccgcc ctcatatagc
    40441 ggtgattacc aatatctatg aagcacatat tgattatcat ggtacaagaa aagaatatgt
    40501 caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa gattatctga tccttaactg
    40561 gaatcagtca gaactacaag aattagcgca aagaacaaaa gcacgcgtac tgcctttctc
    40621 gacaaaagaa gtgttggaag acggtgtgta tgctgacgat tactcaatat actataaaaa
    40681 agaaaaaata atggaaatat ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc
    40741 actagctgct atttctgtag caaaattgta tggtatatca aatgaagcta tacgagaaac
    40801 gttgcatttt ttccatggcg taccacatcg tacgcaatat gtaggagaaa tccaaggcag
    40861 aaaattttat aatgattcaa aagcaaccaa tattttagcg actaaaatgg ctctaagtgg
    40921 atttgaaacg tctaaggtcg tgttattggc tggcggatta gacagaggaa atacatttga
    40981 tgaattaatt ccttcattaa aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga
    41041 aaagttgatg gatgctggaa aaaaagcagg catcgagaca atagtaacag ccgattctgt
    41101 agaacaagca gttcctttag cattagaaaa cagtacagat ggtgatgtag ttttattatc
    41161 accagcgaat gccagctggg atcaataccc aaattttgaa actagaggaa acagattcat
    41221 ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt
    41281 tggttacagg cggaggaaca gggggacata tttatccagc tcttgccttt gtcaattatg
    41341 taaagactca agaaccgaat gctgaattca tgtatgtggg agcaaaacga ggtctagaaa
    41401 acaaaatcgt ccctgataca ggaatgcctt tccatacctt ggagattcaa ggatttaaac
    41461 gaaaaatttc catgcataac atcaagacgg tccgattgtt tcttaagagt atccgtcaag
    41521 caaaaaaaat cctcagtgat tttcaaccag atatcgttat cggtactgga ggatatgttt
    41581 ccggagcggt agtgtacgct gcagccaaaa tgggtattcc aacagttatc catgaacaaa
    41641 acagtgtacc aggtgttacc aataaatttt taagccgata tgtggatcgg atcgctcttt
    41701 catttaaaga tgcggcttcc ttttttcctg aaaacaaagc agttcttgta ggtaatcctc
    41761 gagcacaaga agtagcagat actaaaaaat cagatatttt gaaaacattt gatttggatc
    41821 cagaaaagaa aacagtcttg atttttggcg gtagtcaagg agcacttaaa atcaatcaag
    41881 cagtatcagt ttttttaagt gaatatattc cttcttctta ccaagttttg tatgcttcag
    41941 gagatagata ttatgaagag atcctaaata aaataaatcc tgaaatacaa aatattagtg
    42001 tccagcctta tatcaaaaat atggcagagg tcatggcttc cagtgatctg ctggtggggc
    42061 gtgctggtgc aacttcgatc gcagaattca cagctctggg gttaccggca gtgttggttc
    42121 ccagcccata tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag
    42181 gagcagcaaa aatgattgca gataatgaac taacaggtga atcactaagt cagacagtca
    42241 acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag
    42301 gaatccctga tgcttcaaaa cgtctttatg acttagtaaa acaaataatc aaaaaataaa
    42361 ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga
    42421 tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag aacaagagat
    42481 gaaaaaccaa gagccaaaaa aagaagacat aacattagat acaccccttt cagattctga
    42541 agaagaaaaa acggttcaag aggaacagca agaagaaagc cagtccgaga aaaaaatcga
    42601 attttttgaa gagatcaagc aaaagaaaaa cggtggacca tataacggtt cttttttgaa
    42661 ccggctgccc aatttaaaaa gtcaacggca caaagtacta tttcgtcggt tagctttgat
    42721 catcactgta ttgggtatac cgctgatttt tttgatctat tatgtttctc cttatagcaa
    42781 attacaagca gttgcagtat caggcaataa gacagtcaac agccaagaaa tcatctctga
    42841 tacaaaattg tcattaggag aaaatgtttg gggacagtat tttcatcgat ctacttatat
    42901 cgatcgcctt aaaaaagcac agccaagaat cgaaacagca aatatacatt tcaaagggat
    42961 gaatgaattc gatttggatg taacagaata taaagagatt gctttgatcg caaaaaacaa
    43021 tcaatactac ccagtgattg aaaatggaac agtattagat gaaaaagtag caaatcctac
    43081 aaaaaatctc cctatcttgg aagagttcaa agactctgca aaaatcaaag aattaacaaa
    43141 gcaatacaat cagttatcat cagaattaca aaaagcaatt tctgaaatca agtacacgcc
    43201 aagagcaagc aataaaaatc tgatccaatt gaatatgaat gacggcaatc aagttattgt
    43261 aaatatcaat aatcttgcga accaaatgaa atattattcg caagtagcca aagatatgga
    43321 tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg catccttacg gtagcactca
    43381 agaaacggaa gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa atgcggatca
    43441 aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca caaacacctg gatcgtctag
    43501 cacaaacgga tcatctgatt caaatgattc aagtgaaaca agtcaaatag aagataatcc
    43561 aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa agtaagaatg
    43621 tactttctaa aaaccagtaa tttatggtag aattggaagc agtgaatata tctattaata
    43681 agtatatttt aaaacggacg ctagttgttc gcttgtatat ggaatagtaa gtaggaggga
    43741 atcccattca tggcaaaaac aggaatgtat gtaggccttg atattggaac gacatctgtt
    43801 aaagttgtcg tggctgaata tatcgacagc caaatgaata ttattggagt aggaaacgca
    43861 aaatcagaag ggattaaccg aggcattatc gttgatatcg ataaaacggt ccaagcgata
    43921 caacgagcag tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc
    43981 ggtttgccag caaacatgct tgaagtagaa aattgccagg gaatgattgc agttaacgga
    44041 gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt
    44101 tcaatacctc ctgaacgtca aatcgtgtca atattgccac aagattttac tgtcgatggg
    44161 ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat gtacggtctg
    44221 ttgtttacag gacctaaaac gatcatccat aacatacgta aatgcgtgga aaatgccgga
    44281 ttaatcgtca acgaaatggt gattactcct ttagcgctga ctgaatcaat tctttctgac
    44341 ggagaaaaag attttggtac gatcgtcatt gatatgggtg gcggccaaac aacaacggct
    44401 gttatgcatg ataagcaatt gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc
    44461 actaaagata tctctattgt attaaatacg tcatttaaca atgcagaagc gttgaaaata
    44521 aactacgggg atgcataccc agaacggaca tcaccagatg aagaatttcc agtagatgtg
    44581 attggtcagt cagaaccagt gaaagtcgat gaacgctact tgtcagaaat tatttcagct
    44641 cgaatggaac agattttcaa caaagcaaaa gatgcattgg accaaatcga agcattagag
    44701 ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt tgtggacttg
    44761 gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga
    44821 aacccggtat ttacaaatgt aataagtatc gtggattatt cagcaaactt gagtgaagtg
    44881 tatcaattgg caaaaattgc tgttacgggt gaaacctcag cagctcgtca aatggtagtt
    44941 gaacaagaaa caacaaacac atatgagagc tatgaagcac ctgaagaaac aatctacgat
    45001 gagccggaac caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg tttctttaca
    45061 aatatttttg attaattcat cctgggagga ataagatatg gaattctcaa ttgataataa
    45121 tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt
    45181 aaaccgaatg attgaagaaa acgttaaagg tgttgaattc attacagcca acacagacgt
    45241 gcaagcatta aaaaattcaa aagctgaaac agttatccaa ttaggcccca aatatactcg
    45301 tggtttaggt gctggttctc agccagaagt tggacaaaaa gcggcagaag aaagtgaaca
    45361 atccttacgc gaagctttag acggtgcaga tatgattttc atcactgcag gaatgggtgg
    45421 aggaaccggt acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt
    45481 gactgttggt gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg gccgttttgc
    45541 tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca ttattaatta tctcaaataa
    45601 ccgtttatta gaagttgtag ataaaaagac accgatgctt gaagctttcc gtgaagcaga
    45661 taatgtttta cgacaaggtg tacaaggtat ctctgatttg atcacagcgc caggttacgt
    45721 taaccttgac tttgctgacg tgaaaacagt gatggaaaac caaggtacag cattgatggg
    45781 gatcggtgtg gctagtggag aagatcgcgt agtagaagca acgaaaaaag caatctcctc
    45841 tccattgctt gaaacatcta ttgatggagc tgaacaagta cttcttaaca tcacaggcgg
    45901 tttggatatg acattgttcg aagcacaaga tgcttcagat atcgtagcaa atgcagcaac
    45961 tggcgatgta aatatcattc taggtacatc tatcaatgaa gaaatgggcg atgaaatccg
    46021 tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc
    46081 agctagacaa gcacaaatgc aatcacctgc tcaaaagact gtattagata tggatcaagc
    46141 taaaccaact tcttcagaag aagaaaacag ttttggcgat tgggatatca gacgtgaaca
    46201 aaatgttcgt cctagagttg atgattccaa ttttgacaat atcgaaaaaa aagaatttga
    46261 tacattcaac cgcgaagaaa caaaatctca tggcgatgat gaattaaata cgccaccgtt
    46321 tttccgtcgt aaaaaataaa ggagaggaag agcacatgat tgctgacaac ttgcagaaaa
    46381 tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat
    46441 tagtagctgt aacaaaatct gttgaatccg ctcaagctga agaattggtt agagctggcg
    46501 ttatgaattg tgcagaaaac cgagtagata aattattaga aaagaaacaa gatttatttg
    46561 attttcctga aataaagtgg catttaattg gtaatttaca gcgcagaaag gtaaaattga
    46621 ttgtaaatga aattgattac tttcatgcgt tggacagttt aagattagca gaagaaatcc
    46681 agaagcggtt agagaaagaa ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat
    46741 caaaacatgg aatttcacca gatgagcttt taccgtttgt ggaatcttta gctgcctgca
    46801 caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa agaagcttct gatgagcaaa
    46861 tccataaagt gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg
    46921 cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca agactttcaa attgcgattg
    46981 aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt caaagacgaa taaaggagga
    47041 acaaaaatgc cgcttttaaa cagaggagca attgctaatt ttttcggatt agctgatgag
    47101 gaagagtacg aataccaaga ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa
    47161 aaaaatacaa cacagaatca atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa
    47221 caaaaaacgg cgacttcacg tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa
    47281 aaagtaattg aactacatca atcacctaca aaaactgtag cgaaagaaag aacagcttca
    47341 agaacctcta cacaacctaa agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga
    47401 gcttattctg aagctatggc gattgctaaa cgaatcattg ctggagaagc aaccttagtg
    47461 aattttcatt tagtagaaga aaaacaagcg agaagaatcg ttgattttct aacaggaaca
    47521 gtttatgcac tagatggaga tatcaaacgt gttggagatg agatttttct ttgtacaccg
    47581 actgggatgg agattgacag ttcaaccgct caatcttttg cagatgcgaa tttgtttgat
    47641 ctgtaagagg aggaactacg atttatacta ttgcactatt acttcattta cttcaacggg
    47701 cagcctattt ctatagcatt atcttgatgg tatatgcatt gctctcttgg tttccaggcg
    47761 ggtatcaatc agggctagga cgattattag ctaagatctg cgaaccgtat atcagtttgt
    47821 tcgatcgtct gcctttgagg attggaccct tggatttcac aattttaggg gcaatcctga
    47881 tcttgaactt agccgtacaa ggtttagcac agatcgttat atggataact gtccatttag
    47941 tttattgatt gaagcaacaa aatctttatt ctcagaatag ataggagggg aaaattttgg
    48001 atgcaaatgt ataccagcac tttcgggcag atgaacgtac atttatcgat tcggtaggcg
    48061 attggattga acaggttcaa ggacaatatg caccctatct gaccgaattt cttgacccaa
    48121 gacaatccta tattttagaa acactgattc gacaaagcag tgatttgcgt ttcatgtttt
    48181 atggcggata tgaacaggca gaaagaaaaa gatgtttgat tttccccgat tattacgaac
    48241 ctgtagagga agattttgaa acagcattgt atgaaatcca ttatcctagc aaatttgcca
    48301 gcctaagtca tgggaaaatc cttggaactt tagtgggaac ggggatcaaa cgctcttact
    48361 ttggcgatat catctcggac ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc
    48421 attttgttgc tgcacaagta acaaagattg gaaaggttgc tgtacgcttg gaaccaatca
    48481 aatacacaga aatgattatg ccgaaagata gctggacaca agaaagagga acagttagtt
    48541 ccatgcgact agacagtgtg atctctgaga tttataatat ttccagacag cggtccaagc
    48601 agctgatcga agctggtaaa gtaaaagtga actggacaga aaatacaaga cctgattttg
    48661 tacttgaact attagatatt atttcagtca gaggttatgg cagagtccag atccaagaac
    48721 ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa
    48781 ataaaaaggt ggtttactca tggctttaac tccattagat atccaaaata agacattccc
    48841 gacaaaaatg cggggatata atcaagacga agtagatgat ttcttagatt tagttgttcg
    48901 tgactatgag gaattgactc aaagaaatcg agaattagaa aaagcagtca aacattctga
    48961 agaaaaactt gagtacttca atgaattaaa agatgctttg aaccaatcaa tcatcgtagc
    49021 tcaagatacg gctgataaag taaaaacaag tgcaagcaag gaatccgaag tgatcgttac
    49081 ttctgcacaa aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt
    49141 aacaactgat gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag
    49201 acagcttgcg acagaaactg aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat
    49261 cagcttgatg cttgagtctc aattagaaca agtgaaaagt ccggaatggg atgaaatttt
    49321 acaaccattc tccagctacg tgactgacag ccatgaagtg atcaaagaag ttttagcaaa
    49381 acagcttgac aatgaaaatg atactgatgt aaactcagac acaggagtca tagaagcacc
    49441 tatgacggca tttgatacac aggctattga tatctcgatc gtgcctggtg aaaatgaaaa
    49501 cgagtagaac agaaaagcac tttgttattc ttaaagcgag ccgatgagag tggaagttcg
    49561 gtaagacact gcatggtgaa agatcctcta cttgagacct gcttttccga aattagagta
    49621 agaagcagcg gctgacctcg ttattggtca cagagagttt gcactagata gatgcaacta
    49681 aatttgggtg gtaccacggc atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg
    49741 gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa
    49801 aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg
    49861 ggaagaaaat cgaatctacc aaaaaagaca agagttgaac gaaggaaaac ctagctttgt
    49921 tcttcatgat ggacctccat atgccaatgg taatatccat ttaggacatt cattgaataa
    49981 aatcagtaaa gacattatca ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt
    50041 tccaggatgg gatacacatg gattgccgat tgaacaagta ttggctaata aaggcgtgaa
    50101 acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg cactttccca
    50161 agtagataag caacgagcag atttcaaacg cctaggagtt gcaggagatt gggaacatcc
    50221 ttatgttaca ttagatcctt cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc
    50281 tgaaaaaggc tatatttaca aaggattgaa accaatttac tggtctccgt caagtgagtc
    50341 ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa tctccatcta tttttgtagc
    50401 tttcaatgtc aaagacggca aagatattct tgatgaagat acagcttttg tcatttggac
    50461 gacaacacca tggacattgc cagcaaacca aggtattgct gtaaatccaa aatttacgta
    50521 tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt
    50581 ccaatcagaa atcggttggg aaaatgtaac agttctaaaa gaatttgctg gacaagaaat
    50641 ggaatatatg actgcaagtc atccattcta tgaacgtgaa tcacttgtga ttttaggcga
    50701 tcatgttact ttagacgctg gtactggatt agtccatact gctccaggac atggggaaga
    50761 tgactatatc gctggtagca aatataaact atctgtagtg tcacctgtag actcaaaagg
    50821 attgtttaca gaagaagcac ctggatttga aggtatcttt tatgacaaag caaatcctat
    50881 gatcactgat ctattaaaag aaaaaggagc tttgctgaag ctagatttct ttactcatag
    50941 ttatcctcac gattggcgga cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt
    51001 tgcatctatc gataaattcc gtcagaatat tttggatgaa gtagaaaaag tagagtgggt
    51061 tatcccatgg ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg attgggtaat
    51121 ttcccgtcag cgtgcatggg gagtaccact tccgattttc tatgctgaaa atggagaagc
    51181 aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac atggatcaat
    51241 tatctggttt gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc acccaggttc
    51301 tcctaatggc gaatttacaa aagaaaaaga tatcatggat gtctggtttg attctggttc
    51361 ttctcatgaa gctgttttac gtcagcgacc agaattatca ttcccagcag atatgtattt
    51421 agaaggatca gaccaatatc gtggctggtt caactcaagt atcacgacta gtgtggctat
    51481 caatggtgta gcaccataca aatcagttat ttctcaagga tttgctttag atggtgaagg
    51541 ccgtaagatg agtaaatctt taggaaacat cattgcacca gaaaaagtga tcaaacaatt
    51601 tggtgcagat atccttcgct tatgggtatc tagtgtagat tatgaagcag atgttcgtgt
    51661 ttctatggat atcttaagtc aagtggcaga agtatatcgt aaaatcagaa atacgatgcg
    51721 tttcttatta gcgaatacag aagatttcga tcctaaaaaa gatactgttt cgtacaatga
    51781 tttacgttct gtagacaaat atatgaccgt tcgattgaac caagtaatca aagaaatccg
    51841 agaagaaggg tacgacaaat ataacttcat gcatatttac cgtacagtta tgaacttctt
    51901 gacggttgat ctatcgtcat tctacctgga ttttgctaaa gatgtagttt atattgaagc
    51961 agaagatgat tatcaacgtc gttgcatgca aactgttttc tatcaaacag ctgtagcttt
    52021 gacaaaatta ttgactccta tcattccgca tacagcagaa gaaatctggt cattcctgaa
    52081 agaagaagag gagtacgttc agttaagcga gtttcctggt tatgaagagt ttgcaaacca
    52141 agaagaattg atggatacct ggactgcttt catggacttc cgtgataatg tcttaaaagc
    52201 attggaagaa gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa aactaactgt
    52261 ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt
    52321 attgatcgtt tcaccagact actttgaagt tatggcagca gatgaacaag tgccagataa
    52381 cgctatggta tttgatgatg ttgctattac agtggaaaaa gcagacgggg aaacttgcga
    52441 tcgctgtcgc caagttcgta aagatgtagg agtagatgaa aaacttcctc atctatgcgg
    52501 tcgttgtgca aaaatcgttg aagacttcta tcctgaagct gtagctgaag gattcgaaga
    52561 aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca cacccccttt tgttatcaat
    52621 cgtttaattt tccacgttca cgataccagt tatctggttg ccagatccat tcgaatccgt
    52681 ctttttcaag tagttcgaaa gctgctttag gtcccattga tccagctttg tagttgggaa
    52741 aatccggttg tgtttggtcc caagcttttc gaatgacatc cacgatatgc caagattgag
    52801 cgacttcttc ccaatgagag aagttcgttc catcaccatt caacgcatct aatagtaatt
    52861 tttcgtaagc ttctgggcta ttttcaacca tttctgcact atttctaaat tccaatttga
    52921 ttggttccgt ttcaaatcct tgccctactt ctttcccatt caaggataaa gaaaatcctt
    52981 cagttggctg aatatagatc gttagaacat taggtggaag tgtcgtatcg tcacatggtt
    53041 catcgacaga tgttttgaat acattaacag gaacttgttt gaatacgatg ttgatccgag
    53101 tccctttttc agtcaaacgt ttgcctgtac gtacatagaa aggaacacct gaccaacgga
    53161 agttatcgat agtgaatttg cctgcgacga atgtttcagt ctctgattga gcatccactt
    53221 tgtcttcttc gcgatagccc ttgaattgta caccatccaa ttcaccttga gcatattgcc
    53281 cgcgaacgaa gttttctaac acttcttctt cgttataaat gcggatagct ttcaatgctt
    53341 tgatcttttc tgtacggatt tctttttcgg aaaatgctac tggcggttcc attgcaagta
    53401 atgctacaac ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt
    53461 agtagccccc gcgatcttct acaccgaggt tttcgccaaa agtaatttgg atattgtcga
    53521 tatagcgatt gttccattga gcctcaaaaa tattattggc aaaacggata gctgagatgt
    53581 tttggatcat ttcttttcct aaataatgat caatcctgaa aatatcttgt tctgggaata
    53641 cttctctgat ttgctggttc aattcaaaag cagactggta atcagaaccg aacggttttt
    53701 ctatgatcaa acgattgaag ccattttttg tcataatatt ttgtgatttc aaatgagaaa
    53761 cgatcgttcc aaaaaattga ggtgccatag ccaaataata gacacggtta ccgtttaatt
    53821 gatagtgtcc atctaatcgg tcagacaatt cttttaatgt ctgataatgc gttgaatcat
    53881 ttacatcatg ggattgatag taaaaatggc ttgcaaaggt agttgcttct tcatctgaag
    53941 gattcaatgc acggattgtt tctttgacaa cttcacggta gtgctcatcg ctccagggac
    54001 ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt tccttttctg taaaggcgga
    54061 acaaagacgg gtacaattta cgctgggcta ggtcacctgt accgccgaat atcgtaaaca
    54121 atacattttt ttcattagac atgtggatac actcctttat attttatcga caaaaatcgt
    54181 gctggccgcc ttaaagctga cggggatttt ctttctatca ttttggatag tgatcggtcc
    54241 ttcataagct gcgatgtcaa cgatctcata ttcttcgttg atattcaggt caatagtgac
    54301 taagtaatcc aacaattctt tttcatcaag cactcgtgcg atacgcactt ttgtaccgac
    54361 aggataggac tccaatgttt gtcggcgttc ttcatggaca acttcctctt ttttaggaat
    54421 cgatcctcca tgaggacaat gttcaggatg attcaaatag ttttctaaat gatctgcaag
    54481 atgttccgaa gtcacatgtt ccaatacttc agcatcatca tggacttcat tccagctata
    54541 gtctaaatgt tcaactaaaa aaacttccca taagcgatgt ttgcgaatca gactgctagc
    54601 tttttgcagt ccgcttgcag tcaattgtac accttgataa ggggaatgtt ctacaagctc
    54661 ttctttcaca agtttagaaa tcatttcact gacagatgca gctgagacat ctagactaga
    54721 tacgatttgt ttattgttga ctttcgttgt atctccacct agttctaaaa tgattttcaa
    54781 ataatcttct cgatttggtg tcatattcga tcatcctttc atggtaaaaa accatcatta
    54841 gtaagtgtaa caaaaaaaac gttaattgac tatttttcta aataaaaaac aaaggatttt
    54901 tagacgtttt tacaacaaaa aacgaaagat gatagagaag actcattctt tcgtttataa
    54961 aggatatata tatacattgg gagggatcta tttttcagat aacaacttat atgaagtgtc
    55021 tgcagattcg tcataaagag tttgttcggg ataagaaaag ataagataaa aaccatactg
    55081 atcacgtacg actaaacgat attcatctat ttctaccaca gatgtgccaa gagactgacc
    55141 gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct ggctatcact
    55201 aaagaaccat gtaccttgat actttgagag tccttctgac ttttccgctt gttttttctt
    55261 tttcaagtaa ataaaacttg agagaacggt aatgagtgaa ccaagaaaat agatcatttt
    55321 ttttatcacc aaatcactcc tttatgagaa aataatcgca tagacatatt ctttcttctt
    55381 tatttcattg taccgaataa aagctaaaat ttctctaaga ctttcgactg gtttttttac
    55441 tgtttttcta caaattatga taggatacat agtaggaaga aaggtgacat aatttgaagt
    55501 atcagcaagt ggaaatagct ttttttattg accgcccgaa tcggtttata gcacattgta
    55561 tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg
    55621 tcctgatacc aggcgctgaa gtggctattg tatttgctcc tggacctaaa cggaaaaccg
    55681 cctatgatct gattgctgta aaaaagaagg gtgactggtt taatatcgat agtcaattgc
    55741 ctaatcggct ggcaattgat ggtattttag acggcacgat ccttttacca ggattaactg
    55801 gtccatttac ggtatacaag cgtgaagtaa cttatcttca ctccaaattt gatatttatt
    55861 tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata
    55921 aagcgattgg tgcttttcct gatgcgccca cactcagagg attgaaacat ataggagaat
    55981 taacgtatgc cgcacaagat ggttatgccg cttatgtctt gtttattgct cagttcgaac
    56041 atcttcatct ggcaacgatc catgaagaaa tgcaaccagc actagctgat atggttcgac
    56101 atgcgcagca aagcggagtt caaatcctcg cctataattg tcaagtatcg gcagatgagg
    56161 taactgtcaa gcaggccatt ccatttgatg tagatatccc ttttgaagat cctatacaat
    56221 agaaagaagg taaaataatg atcaacttag gtattatcgg aacaaattgg attactcatc
    56281 aatttgtcca agcagcttta agcacaaaaa aatatgaatt gacagcggta tattctagac
    56341 atttggaaaa agcacaggaa tttggttctc attatgaagg ggatatcgaa ttcgcaacag
    56401 atttgaagac ttttttcaat attactcata tggacaccat ttatattgct tctccgaata
    56461 gtcttcactt tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa
    56521 agccagcttt ttcgacgcca aaagaaatgg atgaaatcat ccatttggca aatgaacaca
    56581 atgtgtattt ttttgaagca gcaagaaata tccatgaatc aggttttaaa aagattgccg
    56641 attacttacc attaaaagac gagataatcg gtgcaaattt cagctatatg aaatattctt
    56701 cgcgctatga ccaagtatta gatggagaag aaccgaatat cttctcgact catttctcag
    56761 gtggtgcatt gatggattta ggtgtttacc ttgtatatgc agctgttgct tggtttggaa
    56821 tgccaaaaga agtccattat ttcccaagaa agatttctac tggtgtagat ggtcttggat
    56881 gggggatctt acgttatgag aattttgatg tatcgatcca gccaggtaaa atcggtgatt
    56941 cttatttacc gtcagagatt tattttgact caggaacgct agtgatgaat ggagtcaatg
    57001 caattgaaca agcacaattt catgatcgcg cgcataaaga aatcattgat ttggcggtaa
    57061 cagctgcaga aaatccaatg gcagatgaag cagaggattt tgccaatgtc atggctcatc
    57121 cgaaagatcc agcttgggga ttgttgtatg aagagtggat cgagctagct agaaatgtga
    57181 accaagtgat ttatgattta agaaaaaatg gcggcatcaa attcgatgca gataatgagg
    57241 aagattttta atgaacgaat ttgtacaagt atttcccgaa aattggcaga aaaaatatga
    57301 ggataaaggg tttatctcac cttctcttat tcaacgagca gtttttcagc cactaagtaa
    57361 taaacaaagt atagtagcgg tttctcctac aggttcaggt aaaacactgg cttatctttg
    57421 gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc
    57481 tcaagaacta gctattcaag tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt
    57541 gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt cagattgagg gtttgaagga
    57601 aaaaccagaa atcttgattg gtacaccagg aagaatattg gaattgatga aagcaaaaaa
    57661 aataaaagca catcaagtga aaacaatggt atttgatgaa gcagaccagc tatttgacgc
    57721 tgggaacagc caaatcattg atcaaattct tcatcaagct ccaacagagt atcagctagc
    57781 ttttttttca gcgacagcag atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac
    57841 gtttgagaca attgacgtga cagcagagga cgattctcga aaaggactga gacattactt
    57901 tttacgtgta ccgatacgaa agaaagatga ctacttgaga cgtctcacgc atatagaaga
    57961 ttttcatggt ctgattttct ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt
    58021 gctgtataga ggtgtaccgg taggaagtct ggcctctgat cagaacaagc tactccgaaa
    58081 agcagcgcta gaacaattca aaaagaaaat ggtcagagca cttttgacca cagatgtggc
    58141 ggctagaggt ttagatatta caggtcttcc ttatgtagtg aatgcagaag ttccgttaag
    58201 tgaagaagct tatttgcatc gttcgggaag aactggtcga atgggtaatg aaggaatagt
    58261 aattacactc gtacaagata ataatctaag agatttaaaa cggcttttac gtccatcaaa
    58321 tataacactt gaagagattt ttttatacgg aggtaagctg caaactgagc agccaaccaa
    58381 agaagatgca gctcctgcag taaataaaaa gtatgcgaaa actttttctg gaaaggagca
    58441 gaacccagaa tataacaaag aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga
    58501 gagaaataaa ggagcaaggc gcaaataagc gcctttttat gctatgatta tgaaaacggg
    58561 aacaaaattg gttgccagag gaggggttat gtgaaagaac gtcaagaaga gatcttgcag
    58621 ctattgacag aaaaccaaca aggattgaca gcaagtgatg tagctgagcg cttaactatc
    58681 gatcgaagca atgcaagccg atatttaagt gaactataca aggcccatca tattgtaaaa
    58741 accgctggcc gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg
    58801 catgtcgact cttctacgca agtgactttt gaaacactcg ttggagaaaa tgattcttta
    58861 aaagtcagca tacagcaggc aaaagctgcg attctttatc caccgcgggg gttgcatacg
    58921 attatttttg gagaaactgg tacagggaag tcgatgtttg ctgaatgtat gtatcatttt
    58981 gcaatcgatt cagagatgct ttctgcagat gcgccatttg tttctttcaa ttgtgcggat
    59041 tatgctcaaa atccgcaact gctctttgga cacatctttg ggatcaaaaa aggggcttac
    59101 acaggagcag ctcaagatag tcccggtcta atagccaaag cagacggcgg tatattgttt
    59161 ttagatgaga tccaccgttt accgccggaa gggcaggaaa tgctgtttac tttcattgat
    59221 aaaggtattt accgtccgct tggagaaagt gcacaagtac atgaagcatc tgttcagatc
    59281 attggggcga cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg acggattcct
    59341 atggcaatca ccttgccgaa tcttgcttca cgttcactgg atgagcgtta tgaaatcatc
    59401 tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa
    59461 gcgattcttg ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac
    59521 ttgaaactag tctgtgcaaa atctttttta cattaccgta ctcatcatga gaaaaagcta
    59581 atcattcgaa aagaagaact ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa
    59641 ataccggaac gtctcgaccg attcatggac agcaaaagtc agtatttgac atttgaacca
    59701 ggatttgctg atgttgtctg gtcacaagat ccagaacgaa acatgcaggt ctataacgat
    59761 attgaggaaa aagtcacttc tctttccgaa accggtgtgg agaacattga tctagagacc
    59821 ctcatttcaa aagatgtgga tgcttatttc cagacatatg tcaaagaatt gactaaaagt
    59881 tcgattccca aagatctttt gccagatgaa atctggcggt taaccaatca attgtatgat
    59941 gtggctgaaa aacggttaga ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg
    60001 catctgcaaa gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg
    60061 aataacgtac ggaaaaattt taagagggaa ttccaggtag caatcgatct ttcaacgatc
    60121 attgaagaag aagaacaagt tgagattcct tttgatgaaa tcggctttat tagtatgttt
    60181 ctttctatca atctaggaga aagtgagcct gttaaagaaa acaaagtaga tgttctcata
    60241 ttgatgcacg gcagacagac tgcttcaagc atgttggaga tggcacaaga actattagga
    60301 acaaaagtag ggaaagcttt taatatgcca ctggttatgg aagttcaaac aatgtatgaa
    60361 gaagtgttac aatatgtcaa aaactatcaa gagcatttga cgaatggcct actcttgtta
    60421 acagatatgg gatcattaaa caccttcagt gatttgattt atgaagagac tggcattcgg
    60481 acgaaagcta tcacgatgac aagtaccatg attgtgatag aagcgatacg gatggcaagt
    60541 gttggaagaa gcttagaaga tatttatcaa aatatccagc tttcctttga atcgatcgta
    60601 cgtgatcagt tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg
    60661 acatgcttta ctggtgaagg agtagctgcc aaattatatc agcgtattat gccagtgatt
    60721 aatcaaaata aggtagaact gatccagatg cagtttttag aacgtgaatc attcaaaaaa
    60781 cacatcgatg ccttattgga agaatatgag atcaaagcaa ttgcgggaac agttgatgtt
    60841 gattatcaaa atatcccatt tttttcagcc tatgacgttt ttgatgatga aaaactaaat
    60901 attttaaagc gtatagctga tgatgagatc ccgacagcta aaattgtcga ttcacttgct
    60961 ggatcattaa gcgatgtctt ttctattgat gaactgatcc gttcattgca aaagacagtt
    61021 cagcagatcc agaccgattt gtcgattatc gtggaacctg gagtagatac tggaatcgtt
    61081 atccatttag ccttcatgtt agacggattg atcaaacaag aaagccatcg gcaatttgag
    61141 aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg
    61201 atggcactag aacgaaaata tcaggtaaaa attccagaag atgatatcgc atttcttaca
    61261 caaatgtttc ttgaaaataa aataaaaaaa tgaacacact aatcatactg tttacacact
    61321 aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct atttcagctc
    61381 ttttttttat tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt
    61441 aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca gggatgagta cgagtttatt
    61501 ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg gaagccgata tttttgcggt
    61561 ctctgcctca gatgcagata ataatttaga atctaagaat gtcgatgtat tattattagg
    61621 tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc
    61681 attagatgtc atcaatatgc aagactatgg catgatgaac ggagaaaaag ttcttgcaca
    61741 agcagaaaat ctaatgaaat aaatcaagta tgcagttggg ggtataacaa cgtggaagat
    61801 caacaaaatc tagaagccat catggggtta atcatgtttg gcggtaatgc aaagagcgat
    61861 gcaatggaag cgattgcggc tgctaaaatc ggtgattttg agttagcgga taggaaaatc
    61921 gctgatgcag aagaatcact tatacaagcg catcatgcac aaacagacat gttgacacaa
    61981 gaagcacaag gaaatcatat gcaagtgaca ttattgactg ttcacagtca agatcactta
    62041 atgacatcta ttgcgtttac tgatttagca aaagaaatca tcgaacttta tcgtcgcgta
    62101 tacagcgaat aaatacaact tcttttttga acggaagaag aaaaattttt gaacggattt
    62161 tttattttgt tataaggaga actctcttta tataaaaaac ttataggagg aagtaaaaaa
    62221 tgaacggact atcaagttgg atggagaaat acatcctgcc ggttgccgca aaaatcggag
    62281 cgcaaaaaca tttagttgct ttgcgtgatg cttttatcgg catactgccc gcaacaatgg
    62341 cgggatcagt cgctgtaatg atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca
    62401 gtacctatgc tgacagttta gcagcagaca aaggaatctt tgccgtattg aatgtcatca
    62461 tcggaatcaa tggttttgtc tggaacggta cactagcaat cgccggattg atctttgcct
    62521 tttcttgggg gtataatctt gcccgagctt atggagtaaa tgatctagct ggggggatcg
    62581 ttggtttagc taccttgatt caaggtattt cctttggtcc agcattatca gcagcattag
    62641 atgtgaatgt tccttcaaat gtcgcagatg caatcaatag ttctgagatt ggtgcaacga
    62701 ttgcagatgg cacactgtca gttggattat ggggttggct aaaactagat catttgaatg
    62761 gaaacgctta ttttacagtt atgatcatgg gagctatttc agtcattatt ttttgtaaat
    62821 tgatgcttgc caacattacg atcaaaatgc ctgattcagt accaccagca gtatctaaag
    62881 catttgcagc cattttacca gcgacgatcg ctttatacgt cattgctatt atcaattata
    62941 tcgtaggtcg tgtagctgat gggcaactga tcattgattt ggtgcaaaaa tatattgcag
    63001 aaccattcct aggattatct caagggttgg gagcagtatt gatcgtcaca gtatttgttc
    63061 aagtattctg gttctttggt attcatggat caaatgtatt ggcaccagtg ctagagggaa
    63121 tctggggaca agcacaacta atcaatattg atattttcca aaaaggttat aacggaaaaa
    63181 ctggtacacc agccgtgtta gatgcaatcg aagatggaaa agcttatatg tgggtccgcg
    63241 gatcgttcga tgcttttgca tggtttggcg gatcaggcgg aacgatcgtc ttactgatag
    63301 ctatcttgat tttctctaaa cgtgcagatt atctaacagt agctaaatta tccattgggc
    63361 ctggtatctt caatatcaac gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa
    63421 tcatgtttat cccattcttg attgctccag tagtagcaac agcaattgga tggacagcta
    63481 cttatcttgg cctagtcgct ccggtatcgc agcaagtcac atgggtagta ccgccgttct
    63541 tattgtcatt cttagcaaca ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta
    63601 tggtcgtgac tttcttgatt tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg
    63661 ccgcaggcga acaacaataa gatgaacgtg aggagcggga cgctttaagt ttcgttcctc
    63721 ataatgaatt ttcgaggagg aactgaaatg attacaattg ccagtgcaat gcaacaagat
    63781 gaaatcaaag aattgttaga aggatacaat gaagaaggaa tgacttttac atttaaagaa
    63841 aaacaaggaa tcaagctgtt ctttgaaaca agtgcagaag atccggaagt tgcggctaaa
    63901 aaagcaaagg aactaatcaa agcacagcct tggggaacag tcctttattt tcaatcaaca
    63961 gctgtttaga aaatggttta aaaagatagg atggttgaga tgataaaaaa cggtttattt
    64021 ttatgtagta ttggttcttt gatcgtgttg tatagtttga atccacattc gcaaaggtat
    64081 gatttgtata gtatggtcac aggtcttttt ctaacaggat taggaatcta tttcttcatg
    64141 aaggggaaaa aaagagaaga agcaaaggag aaaaataact aatggaaatc acgaaaaaat
    64201 taaatattcc agcatcgttt ttttacgata aagttatgga ctctgtcctt ttcgatgtac
    64261 gtaaagcgac cggcaaatca gtaactagaa aacagctgaa aaatttggag tacgtcaaac
    64321 aattctcgca aaataaccgg gctcgcataa aaatcgaaga agtcattgaa aaccagtctt
    64381 ataagtttcg tacatcgaca acgaagaatg aattcgtcgt tcattatcaa atcaaaccat
    64441 tggacgattc gacctgtgag gttcattaca cagaaaaaat ggagtcttat ggcatgcttc
    64501 aaaagttgaa tgatatgctt ttaggaacaa tcttgatgta ttttaaacgg agacgtttca
    64561 aaaaaatgct agatatgatc gaagcaacgt attaaatcaa tgaaacatgg tactatttag
    64621 ttattgaaaa atgaagtaag ctagaaggaa tttttcatag gaagatagat ctttcctgcg
    64681 aatcctcact agcttttttg taggaagggg cagcaaaatg acaaacaaag taagagagct
    64741 aggtgtatct tcgattgagg aaatgtttga tttagccact tatgcattta atggggcaga
    64801 tacacctgaa aggcgagaac gttttcgtac acttgcggag aattcttgga attacggatt
    64861 ttttgatgag gaggatcggc taacaagtca agtcatggcg accccttttc cagtgaattt
    64921 ttacggacaa gagtacctaa tggccggcat tggctatgtt gcttcttatc cagaagcaag
    64981 gggacaaggc gggatcaatc agatcatgga aaagatcctt gaagattgcc gtgatcgtaa
    65041 agtaagctta tcttatctgg cgccattctc ctatcccttc tatcggcgct atggctatga
    65101 acaatcattt gataaaatca gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa
    65161 aaaatctgga aaaataaagc gtatggattt tgaagatgca aaagctgcaa tcagaagaat
    65221 ttatgaacag atgccggaaa accaacgagc tggtctgaag cgcgaagact ggtggtatac
    65281 gtataaattc aagcaaaaag gcaattacca gtttgctttg cattttgatg ataatgagga
    65341 agcaacggga tatatcgttt atcagcttgc aacgtcttcc tttattattg cggagtgggg
    65401 atttttggat catgatgctt tttgcagcct tgttcgcttc gtaagttctc acaatggtgc
    65461 ttttgaaaca tttgaatatt catgcggcta cggcggtaat gatcaaaatt atctattaga
    65521 aaatccattt gcttctgtaa tgatcacacc ttatatgatg gcgaggatcg tagatatttc
    65581 tttgtttttg gaaaattatc catttaaaaa gagacaagct gcatttgccc tacaaataga
    65641 agaagatcat tatgcgaaat ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa
    65701 aaatcatctc tcaaaagtag aacaaacaaa actcccaatc gttcgaggaa gtatccaaca
    65761 tatcacgcag ctattattag ggtatcgtaa agtggaagag ttggtattcc aagaaagaat
    65821 acaggtgaca aaagaatatg aagaaacgtt aagctcaatc ttgccacagc aacagccaat
    65881 tcttaatgac tatttttgat aaaaaacggg tgatgacaat atttttgtca ccaccctttt
    65941 gtttttcatt tggcatttga aggaaaatgt taaagtaaat cctctttttt cgtaaatttg
    66001 aattttttgt tttcttttgt tttagggttt tcttttttgt ggtcttggat tgcttctttc
    66061 gtaatctttt cccgattttt tttagatgaa tctaagcgtt ctgcgtatac tttttctttt
    66121 ggcttatcat tcattggaaa ggacctccct acattatttc attctacctc tagtttatca
    66181 tctgttaagg actagtggaa acgttttgcc taacttataa gctgattttt ttagcagata
    66241 agcttgcaag taaaaaacaa acaagctaca attgatttat tgaaattgtt cctgtagctc
    66301 agtaggatag agcaatcgcc tcctaagcga taggtcgctg gttcaattcc agtcaggaac
    66361 gtataaataa cccctgtatc ctttgcggta caggggtttg ttttgtattt gtgccaaaaa
    66421 tgtgccaaac tagttatcat ttttatataa actatcgaac acagtattga ttttttcttt
    66481 atcctgatct tcaagctctc gaacaatatg aagataagta gacattgtag tttctaaact
    66541 gctatgtcct aaacgtttcg atacgctaag tatatttact ccttgataaa gtaaaataga
    66601 tgcatgtgta tgccttagtc catgcaaagt aaattgcttt tcaatcccta attcttttaa
    66661 ttttttgcgc aaatatttgc tgacggcgtt tgatgaaact aatccatttt ttaagttgaa
    66721 aaaaacaaaa ttatgagggt ttttgatttc aaaattctcg aataattctt tttgattgat
    66781 tttaaacttt tttaagagat caatcgtatg tttatcaatg gaaattttcc tatttgaagt
    66841 ttcgtttttt gtttttcccc attcatttaa tttataattc catgttttat taatagtgat
    66901 gatttgatct tcgaaatcga tatcttccca tgttaatcct agaagttcag caaatcgcat
    66961 tccagtggca ccagcaacta acacgagcat aggagaagag tactgcgcag aaaggttttc
    67021 ttctacgagt ttcattaaag atttaaattg atcataatct aaatatttat cttcttttgg
    67081 cttcaatgaa tcttttcctt tgattactgc ttttctagtt ggatcaaacg gaattaatcc
    67141 ttcttctacg gcatctttca acgacgctct aatatggtta ttaaacttaa taacggtaga
    67201 ttttacatgg tctttagcat acttatttaa aaagcgttga tatcccactc tatctaaatc
    67261 agaaatcaat accgctggca tatatttctt tatgttcatt aacgtatctt cgtattttct
    67321 ataagtgata ggagatactg ttccttcttt ataaagttgc atccaatcct caaagtaatc
    67381 tgataataat agatttttgc gatccccttt aaggcctttg ttcaagttat attctaattc
    67441 gttggcggca tcttttgctt ctccttttgt cttaaatcct gattttctta tcttcgtgta
    67501 ttttccgtca tcttttttgt atgaaatttc gtattgccaa ctatttccac gtttgactaa
    67561 tcttgccata attgaatcag ctctctttct ctgatacaat aggaactata aagaagccca
    67621 ttgtttgggt tttattattt tttgcacatc ctttttgctt gccggcgcaa ggatgtgcta
    67681 tttgtcgttt taatggtata attggatgtg tattagatca tccaatcaga ggtgtttatt
    67741 atgattaaag tgatttttaa aagaataact gaacttccag agtttagatt atttgttgat
    67801 tattggtatg tctggttttt tattgttgca ttgctgatag gtctagatgt catattagaa
    67861 agaaaactta agtaagagtt gagttaataa attgaattcc taaatgagat attttgtagc
    67921 ccatattttg tttgccgaca tcacctaata ctatggagct aatcgttttt tctaacagac
    67981 caaaatcaga taatctattt aagctaccat ctattagctc ttgtgaatag ccagacatgc
    68041 tgatatttcc agtgaaattt tcgtttccac gactcattaa gcatgctaac aattgaatct
    68101 caatcgatgt taattgattc agtacaagaa gataatactc ctcgttatcc catgaagcgt
    68161 tgttaattcg aagtaaacaa ttcttataaa gatttttata catatctatt ttattttgtg
    68221 cctttgattt ttctacctcg tcatttatcc cttcgaatat tccaatgagt tggtccttgt
    68281 tctctaagtt gaaaacatct tctggaattt gatcgcgaat gttttctaat gattcgttta
    68341 aatttttgta gaatttctcg atgcgtttga aacgcttctc gtttttagac ccaaaataaa
    68401 gtgactgcac aacgccacca atataaggaa ttgctccaat accgccttca actattaatt
    68461 caagatattc ttttttggtt agtttttcat tgctattatc catagttacc tcctattttg
    68521 tcatttcctt tacaagcatt gtgactcctg atggcactct tttgtatagg ttttattatt
    68581 gtgcacaatc taactcttag tggggcgtgg ttcatttatt ttttacttac tttactatta
    68641 atcatatccc attgagcata ggatttttct atgttcgatc ctcttgaact aatttctcct
    68701 gtaaaagtta ctttatcgcc ctttgtaaag gtatcagcat tgtttaagtc cttcgcaaaa
    68761 attacatatg aaactttagg tgttgcagaa atatcattcc aacttttatc atcgtactct
    68821 ttgtctgcta taatggctat tctagtagtc attgattcga taacagttcc ttcaaaagta
    68881 tatgttttac cactaatata tttagtaaaa gctgtatttt gctcagtagc tggagtaata
    68941 gaataataac tatctaagaa agcaccccag tcatgattat ttgtctctaa aaattcttca
    69001 aattgagtta ggcttttatt gctgctgtta gatgtagtag tttgagtatc agatgatgag
    69061 ctttcctgtt tgttattgtc atttgcctcg tttgatgagg aaagcgagaa acctacggta
    69121 gtaaaaataa tcccgataat aaggagaatc ataggtgttt tcttatttgt ttttttgata
    69181 aatcttacaa ttaacatgat taaacctata ataaataaaa caataccagt aaaaccaaat
    69241 ataccgtcca ttaatagatc ctctttctct tttgatattg aatattataa accaagcaat
    69301 tcttttttct taaaatcgaa ttcttcttga ttaataatcc cttcatctaa caactcttta
    69361 tactttttaa tttcatctgc acttgaaatt tgtgtttctt tggaattatt atctaatata
    69421 gattgttgct tctctatata tttttttaaa ttttccattt cattccaata tttttttgta
    69481 aacataacgg tgttttcatc tttcgtggcc gctaatatac ctccgcgact ttcgtttccg
    69541 cctagcaagg taaattgtat ataaccatta ctcatacctg gtttttttaa ttgtacggct
    69601 gatatatttt taaaggggat agttttttct cctttaatac cttgattaac taaatttata
    69661 aatccttttc taataattga aatagaatca tcagttactt taatcaaggt ttttcctgga
    69721 gattttatta aaatctcttt ttccattttt tcttcctcat ttctgtgata tgattttttt
    69781 tgtagaagct catatatgag gaaagagccc gtgttgcagc acgggctttt ttaatgtttt
    69841 gctgaaatcg gtttttttaa taattcttga tgtattcata catattgcct tgcgtaagaa
    69901 tattttttct taaaatagca ttggcagaca gcataacaag tgtatctgag cttattctga
    69961 atagaataat actccataaa tttttcgaga ttaaactgag attcatcagt cagttcgttc
    70021 tcaatataga tatttaataa aattagaata gctattttat ctgcttctgt ttcaaatttt
    70081 gagtgaaaag ttgtagaagt atcgtacaaa actgaaaatt caaaatgaga ggcgctgaaa
    70141 tgtgcaagtt catgagataa atgaaaggct tctgcagttt caccgtatag attttcattc
    70201 aaaaaaatga ttctgggttt tggatagtag aaaccaggct ctttcatctc catataaact
    70261 actttcaaat tgtattcgct caacatttct ttcaatttca aatacataca aaccatcact
    70321 ccaactattc attttcctct aaagctttag caattgcaat cgctttacgc attgtctcct
    70381 tagatatttc ttttccatca aaagaaaaaa cagtatcgtc ttctgataaa tccacatgtt
    70441 taggggtttc tctttcttct ctacctagaa ggtagtctac agagacatcg aaatagtcag
    70501 caatccgact taattcatct gaattaggag tattattttt ccactttgct agataaccgt
    70561 tagaatatcc aaaattaatt tctaactgcc gtatagatat ctttttcctc ttcgccaagt
    70621 cttttattat ttcataggta ttcatcgata aaacaacctt tctaaatgtt tacaaaaaaa
    70681 gtttagaaaa atacgcagaa ataatttgac taattctgag taatgagcta tactatatct
    70741 tgtaaacaga tttaacaact aaaaagacaa caaaaaacac tattgatatg taaatgcaga
    70801 ccgccaagaa agctttaaaa tcaatgtttt tatgtcttat ttaattatgc tcttagtata
    70861 gagtattact cagatactgt caattgaatt tagaaaaaag ttgttaaatt tgtttacgaa
    70921 tataaaagaa aggaatgaga aatatgaaca caccgcaaat ttttaatttc gaacaaaacg
    70981 aagttcggac aattttagta aatgatgaac catattttgt aggcaaagac gttgcgagtg
    71041 ttttgggtta ttcaaacact aaagatgctt tgtcgcggca tgtagatttg gaagataaga
    71101 tggggtcgcg aatcacgacc tcaggtcaat caagagagat gacaatcatc aacgaatctg
    71161 gtttgtacag tttaatctta aaatcaaaac ttccctctgc caaaaaattt aaacgttggg
    71221 taacaagtga agtgttgcca gcaattagaa aacatggagg ttatctaact ccagaaaaag
    71281 tagaagaagc tttgcttaat ccagatacaa tcattcaatt agcaactcaa ctaaaagaag
    71341 aaagaactgg aagattaatc gcagaacaaa agattgcaga gtacgaacct aaaatctcct
    71401 atttagatag catattatct tctacagatt cagtaacaat tagtcagatt gcagcagatt
    71461 atgggatgtc tccacaacag atgaataaat tacttcataa actaggtatt cagaaaaaag
    71521 tcggtaacca atggttattg tgcaaaaaac acatgaacca aggatacaca aaatctcata
    71581 caactgagat cccgaaagcc gatggtggca ctaaaattgt aatgaatacc aaatggacac
    71641 agaaagggcg tctatttatc tacgaattac taaaaaaaga aggatattac cctcaaatgg
    71701 atttagagga aattggttag aaaggagttt tagtatgact gacattgcag aaatcactca
    71761 acgagataga gaaaaaatca aagaatatgt cgaaagttca aagttcttaa cttacaccat
    71821 gcttgctgaa agatttggaa ttagcaaaag ctacttatct ttaattttaa acggtaaaaa
    71881 gacttctgca gaagcaaaca gaattataga ttcgattatc actatgtacg aattgtaaag
    71941 ggaggaacag ctaatgcaat atctagaagc aaaaatccca attccagaag gctatgtaat
    72001 tatctcccaa gtggattatg aggagttaaa aaaagctgat gatactggta gatggatgac
    72061 gttgccagaa gtgctagaac ggattaacag aaaatatgat tggtttactt ctagagtttt
    72121 aaagaaccca agatatagaa atattatcga tatcgagaaa aacaaaaatg gatttgtcta
    72181 ttatccagtt gaaggaagag acacatatct atttttaaga agtaaaacac ttgaattttt
    72241 agaaacaaat ttttcggaaa tcttaaggag gcaagcggat gggaaaattt aatagagcgc
    72301 tagtgttcag cgcaccgcta atcatctacg ctttaggact ttggggaagc aggcaagcgt
    72361 tgataggaac gattgtttac atggtttgga tttttatggg gcttgatgaa gctgaagctg
    72421 agtacagagc gaaaaagcca accgaggggg ctgactaaaa atgaatagaa aagagaaact
    72481 agaatgtata ttactattac tcagtttaat tctttcacta atttctctat tgggaagttt
    72541 gtatttttga tatcaacaat tttgtggatt tgggttctat ttagagtaaa tatcattttt
    72601 gtttggtgat taaggagttt attaaaatta attttcccta cttcaaatgc aaggtaacaa
    72661 gatatagcac ttttaggagg aatatttagc ggtacagctt cagagtatat tctattatca
    72721 tcgactgatc ctcctttagc aattaatttc ttaaatggag tagctgacca tctgcggttt
    72781 aagttagcgg atgaaagttc taaattaact aacatagctg gttctgttga atagttagaa
    72841 atgattactt ttgtataaat catatctgca gcgaagtaag aagcattaag ttcaacttct
    72901 agttgagggc gcattccttt tatttttatc cctgttaaaa acgtactcaa tataaaactt
    72961 attattgaca tccattgaaa aattgttaag tgaaaaaaat tcaaaataaa caccaccagt
    73021 ttttaactaa attataccaa aaaggagaga agaaataatg caagaattag taattttgaa
    73081 aaataaagaa gctgtgacta cgagcttaca agtggcagaa agcttcgaga aaaaacatca
    73141 acatgtttta agagatattg atgcactaaa aaaagatgtg tccaattttg gacagatgtt
    73201 tgtagaaggt aatgaaccag attcatatgg cagaaatcga cgagttttct tcattagtag
    73261 agatggattt ttcttgctgg ctatggggtt tacaggaaag aaagctatct acttcaaaca
    73321 aaaatacatt gaagcattca acgaaatgga agatgttatt cgcaagaata ctgttcctca
    73381 aacaattgaa gatatgatga tctatcaatt agaagaaatg aaagatgtta aaaaagatgt
    73441 ttccatgctt aaagatacta tgcgaattag cggacaacaa gagtttgaaa ttaagcaaaa
    73501 aggaaatatg aaagttatgg gagttctagg gggaaaagaa agccgagctt atgaagaaat
    73561 cagcaaaaaa gtattctcaa aattttggtc tgaatttaaa cgtacctttt caatcccaag
    73621 atatggcgag ttacctcgta agagattcga tgatgctgtt tcatttattg aaatgtggtt
    73681 gccagaaact gcgatccgta tggaaattga tcaactgaac agacaacaga gactttttgg
    73741 tgatgacaat gaatagagct gaagcgctaa gaatagggac ggcaattgct aattgctggt
    73801 ggaaatacta taaaccaatc atcctaagcc aacaacatat tgacaagcaa aaagcatggc
    73861 aacaaataaa aaagtgactc cgccggcaag caaagagtca caaagaaaac acatcataag
    73921 gagattttag catatggaaa aagaactttc cactctagat caatatttga ttgatcctga
    73981 ttggggcaag ccgaaaattg aggaaacaag tggtcgaaaa atcagacgaa atcttttgac
    74041 gaatgaagaa ctagcttggg atcaagatga tttaggcaac catgtaacta tttgggatca
    74101 tgtttatctt atccatctat cgaagcattc gaataaacct gaatatattt acgtcatcga
    74161 agatggcttg attgatgcgc tagaagagta cgacagagat aacttgattg atatctctta
    74221 ttacggacca ggtaagaaat acattgctga aatggaggca gaatttgatg agtgaaatca
    74281 aagggacaac gaactttgaa aaacttttta gtcgtaagtt aaataaaatt ctcaagaaaa
    74341 aaggaaattt tgattattta tcttgggctc acgcgtggga gattatgaaa aagaatgatc
    74401 cacaggcaac ggtaactatt aatgagtaca aacactacag ggttgtttct ggaactcatc
    74461 aagactttct tgttgaggaa tataaacctt ttcttatgga tgaaactggg acttatgtat
    74521 ctgtctcagt aacggttaaa ggacacacgg aaaccgaatt atttcctgtt ttagattatc
    74581 gaaaccaacc agttgttaaa ccaaatgcaa tgcaaatcaa taactcattg aagcgatgct
    74641 ttgtgaaagc attggctcta cacggactgg gattatatgt atttcaaggg gaagatattc
    74701 caacaccacc tagaatcgat acaaagaaat taaacatgct agagacgatt ctagaagctt
    74761 tcaatgagca gatgggtaaa gatatgacca aaaccttaat cgaatatgtt aatgagcaga
    74821 cagataaatt agggctctta gctgataacg ttgaaactat tgaacagtta agctatgagc
    74881 aatgtgcctt gatggagcga gcaatagcag ctaagagaaa agaattagat aagaagtgat
    74941 atgagtgttt aaaccattaa tcgattcata ttcagcggtt ctgaaaaagt tcaaaggaaa
    75001 agacataagc gcaaccatca atgaggaagt gaacattgat cgactaaaga cgatgtatga
    75061 cggctacgat ggtgatcgag tcattgaaat tcgttttatt gatcctagac gtttcaccgt
    75121 acagcaacga aacttcatct atgcgctgat aggcgatatt tttatcgata caggcatgcc
    75181 aacggacttc tggaaggaat tcttctactt tcgttttgaa ggtgtcacag ggcgcaaaat
    75241 aagcctgaaa gacgaatcga atacaactgt gagtgatgcc aatgtcttag caaatatcat
    75301 cttagatttc atctttgaac atcatattcc tttcaaagaa ggctatgaga ttttacctgc
    75361 gaatcaagaa tattacttct acaaatgcat cacaaaaaga gtctgctgca tctgtggcaa
    75421 aacaggagct gacatcgatc actttgacaa agcgctagga agacgaaagc gcaaagaagt
    75481 tgatcatgca gagtacacat ttgcagcact ctgcagaatc catcacacag agaagcacaa
    75541 aataggtgtg attaatttca aaaataaata tcaaatcaaa gggatcaaat taaaccagaa
    75601 aacaatcaaa aagttaaata ttggagggga aaaatgacag aacatcgaag ttattatgcg
    75661 attataccag ccaacgtaag gtacgacaaa agacttaaac caaatactaa gttgttatac
    75721 ggagagataa cggccttgtg taatgaaaga ggcttttgtt gggcaggcaa tgagtacttt
    75781 gcagatttat atggtgtgaa taaagagacc atatcgcgat gggtaagtga tttgattaag
    75841 tttggatact tgaatcggga aatcatttac aaagagggta ccaatcaaat aatcaatagg
    75901 tacctacgaa ttaatcaata ccctattgac gaaaaacgca atacccctat tgacgaaaaa
    75961 gtcaaagata ataatacatc tattaataat acatttaata atacaaaaga atatataaga
    76021 gagttaccgc cttcgaaaaa atcgaaggct aagcccgtcc gtcataaata cggagagtat
    76081 aaaaatgttc ttttgtcaga tgagcagatg gagaaactca aaacagaatt ccctaatgat
    76141 taccaagagc gaatcgaacg actgtcagag tattgtgaat catctggtaa gacttataaa
    76201 aactatttgg caactattcg aagttgggca aggaaagaaa aaagtgaacc taagaacgca
    76261 agcagtggat acaagcgcac aggaagacga gagaagcttc ctgaatgggc aatcgaccaa
    76321 gaagcctatc tcaagaaaaa agcgctagaa cgagctaata gacaatcaaa agcaccattc
    76381 taagaggtgg aaaaatgaag atcgattatc tagaactaat taatgaaata gcgaattata
    76441 aaaagggcga ggaattagac gtcctgagag acgtatatga tcaactcgaa gaagctggaa
    76501 ttgaaggaat taagaatgat cgttcgagtt ggagtaaact cagatactat ttcgcactct
    76561 atatcgatgg aacgcaatta agaaatttag catataccaa attactattc attgattgtg
    76621 ttaaaggctt gcaaaaacat cttaatgaac ttgagcaggt gtaaacaaga tggacctaaa
    76681 gacatttaca gcacagatcg aattaatgca tcaagaagct ttaagacaaa gtgtgtcgta
    76741 cgaagacaag tggctcaaca catttcatgg cggacgtgag agcgcacttg atcaagtact
    76801 caaattactg aaaggagaat gtcgggatgg ataagaaagc ggcaatgcag cgaattatcg
    76861 aattgactta ttcagaagat tggcaggaag ataaaaaaat agttgcagaa gtccaaaagc
    76921 tcggtaaatc aatgtggact gaaaagccca aacggagaac gccgagaaag attgcaatct
    76981 ggcatggtga tcgaattcta gtaacgggta ccgctaaaca gttatctgaa attactggtc
    77041 tgagcaaaaa tattatttgg gatagagcta ggagcttatg gattgattca aaaggaagac
    77101 agtttaggta tgtggaggag aaataatgga cgaactaatc gcgaaagtag agcagtgggc
    77161 aaaagataag ggattggatc aagcggattt cagcaagcaa atgttaaaaa cggtcgaaga
    77221 aatcggggaa gttgccgctt ctctagctag aaaagatgaa catggtttaa gagacggaat
    77281 tggcgatgta gtagtaacct tgatcatctt ggcaatgcaa aatgatatgg atttatacga
    77341 gtgcttggat tttgcttatg acgaaatcaa aggtcggact ggaaagatgg tcaatggtgt
    77401 tttcgttaag tccagtgacc tgaaataatt ccattaatgg aagaaataac tgaataggag
    77461 gatagacatg attgatgcaa aacaagtgca aaaacagaaa gatggaatgt tgatgtttga
    77521 agcatatgtt ctaccatttc ttaatcaatt tgaagttttg gaatgctctg ccagcggaga
    77581 agaattggag tatgttgtaa ttcgtgaaac caaagaaaac gttcagaagc taaacgaatt
    77641 tctttgcact attaactgtt gggatatgat agctccagga tttttatgtc cagctatggg
    77701 agaatttttg gaatattgtc gtcttgaaga tgcaggcact ttggatttag cttatctggt
    77761 atacaactac ctaaacatca acacagatca tttgtggttt ggcactgccg aaagaaaatg
    77821 ggtagtccgc taaccaccaa tatcaatgaa aaaaggcggt ttcctgaaag tggaattgcc
    77881 gccattgctg taaggttctt tacgcgactc aaggtcacag aaatataaat gcaaatctct
    77941 tttgaagtga tccaaagctc tcggaaaatg gaagtttttt gtttcaaaga ataaatgaga
    78001 tttgcgtcaa gaacctgaga gttatttcca aaaaagcttc caaagaaccg aatgagctaa
    78061 ggacatcatg tgtaaacaca taatatagct ctcctttcac ggctaatcat aacatgaaaa
    78121 atattcgatg tcagtaggaa aaaaggtgaa ttatgaaaag aatattaaat tatcccggca
    78181 gtaacagatt ctgcaattaa actccgaagc tgatgtatca gtatcaactg tagaatatta
    78241 tgagcgtgga gaaagtcaag cagtcacaaa tgtaaaaatc gataacgtca acggtcttat
    78301 ctgtttagat gaaggcgata gtatgaatga aattgctgca gaccatgaat tatcagaaac
    78361 tgctttaatt gatcaaatcg aattagctag acaatacttt gttccatatg cagatacctg
    78421 gtccaagcat aagaatgaca tcatttttcc agaggaacaa tgagagcaac tgatccagta
    78481 attatccttg aggaagccaa atttatttgg actcacgaag agatagagca agcacgcttg
    78541 ctcttttctc aaggagttaa gccgagcaaa gtagctgaaa taatgggtca aaagattctt
    78601 gatgtcggat tgcttttgct ccatctagca gaaaaaattt gatttgaggt gaaaatgatg
    78661 attcaacttg caggcatcca aacagggaaa atttattttt ctggagaaag caaaagcgaa
    78721 gccaaccaat ggctattgaa aacttacacg aacaataaga agctaaggaa aaaatatcct
    78781 gatttgttgc taaaggatga tcagattatg ccggaaccga tgattttgac ccgtaaggag
    78841 ggaagttgaa actaaaaaac c
//
LOCUS       DAZAFF010000004        57244 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000004 DAZAFF010000000
VERSION     DAZAFF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.004"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..409
                     /locus_tag="ACG9BB_000282"
     CDS             179..409
                     /locus_tag="ACG9BB_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFN2836262.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            506..1807
                     /gene="murA"
                     /locus_tag="ACG9BB_000283"
     CDS             506..1807
                     /gene="murA"
                     /locus_tag="ACG9BB_000283"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFN2836263.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1820..1996
                     /locus_tag="ACG9BB_000284"
     CDS             1820..1996
                     /locus_tag="ACG9BB_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFN2836264.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2131..2427
                     /gene="yidD"
                     /locus_tag="ACG9BB_000285"
     CDS             2131..2427
                     /gene="yidD"
                     /locus_tag="ACG9BB_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFN2836265.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2612..3079
                     /locus_tag="ACG9BB_000286"
     CDS             2612..3079
                     /locus_tag="ACG9BB_000286"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2836266.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3164..4048
                     /locus_tag="ACG9BB_000287"
     CDS             3164..4048
                     /locus_tag="ACG9BB_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN2836267.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4162..4398
                     /locus_tag="ACG9BB_000288"
     CDS             4162..4398
                     /locus_tag="ACG9BB_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFN2836268.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4486..4566
                     /locus_tag="ACG9BB_000289"
     tRNA            4486..4566
                     /locus_tag="ACG9BB_000289"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4520..4522,aa:Tyr,seq:gta)
     gene            4573..4644
                     /locus_tag="ACG9BB_000290"
     tRNA            4573..4644
                     /locus_tag="ACG9BB_000290"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4605..4607,aa:Gln,seq:ttg)
     gene            4769..4939
                     /locus_tag="ACG9BB_000291"
     CDS             4769..4939
                     /locus_tag="ACG9BB_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836269.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5159..5866
                     /locus_tag="ACG9BB_000292"
     CDS             5159..5866
                     /locus_tag="ACG9BB_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2836270.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5925..6569)
                     /locus_tag="ACG9BB_000293"
     CDS             complement(5925..6569)
                     /locus_tag="ACG9BB_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFN2836271.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6695..6982
                     /locus_tag="ACG9BB_000294"
     CDS             6695..6982
                     /locus_tag="ACG9BB_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836272.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7090..7410
                     /locus_tag="ACG9BB_000295"
     CDS             7090..7410
                     /locus_tag="ACG9BB_000295"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2836273.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7491..8804
                     /locus_tag="ACG9BB_000296"
     CDS             7491..8804
                     /locus_tag="ACG9BB_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFN2836274.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8801..9520
                     /locus_tag="ACG9BB_000297"
     CDS             8801..9520
                     /locus_tag="ACG9BB_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFN2836275.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9648..10199
                     /gene="raiA"
                     /locus_tag="ACG9BB_000298"
     CDS             9648..10199
                     /gene="raiA"
                     /locus_tag="ACG9BB_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFN2836276.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10421..12955
                     /gene="secA"
                     /locus_tag="ACG9BB_000299"
     CDS             10421..12955
                     /gene="secA"
                     /locus_tag="ACG9BB_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFN2836277.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13081..14182
                     /gene="prfB"
                     /locus_tag="ACG9BB_000300"
     CDS             join(13081..13152,13154..14182)
                     /gene="prfB"
                     /locus_tag="ACG9BB_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFN2836278.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14287..14973
                     /gene="ftsE"
                     /locus_tag="ACG9BB_000301"
     CDS             14287..14973
                     /gene="ftsE"
                     /locus_tag="ACG9BB_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFN2836279.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14966..15850
                     /gene="ftsX"
                     /locus_tag="ACG9BB_000302"
     CDS             14966..15850
                     /gene="ftsX"
                     /locus_tag="ACG9BB_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFN2836280.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16002..17450
                     /gene="cls"
                     /locus_tag="ACG9BB_000303"
     CDS             16002..17450
                     /gene="cls"
                     /locus_tag="ACG9BB_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFN2836281.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17787..18644
                     /locus_tag="ACG9BB_000304"
     CDS             17787..18644
                     /locus_tag="ACG9BB_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFN2836282.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18759..19679
                     /gene="pstC"
                     /locus_tag="ACG9BB_000305"
     CDS             18759..19679
                     /gene="pstC"
                     /locus_tag="ACG9BB_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFN2836283.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19679..20563
                     /gene="pstA"
                     /locus_tag="ACG9BB_000306"
     CDS             19679..20563
                     /gene="pstA"
                     /locus_tag="ACG9BB_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFN2836284.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20578..21384
                     /gene="pstB"
                     /locus_tag="ACG9BB_000307"
     CDS             20578..21384
                     /gene="pstB"
                     /locus_tag="ACG9BB_000307"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFN2836285.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21400..22158
                     /gene="pstB"
                     /locus_tag="ACG9BB_000308"
     CDS             21400..22158
                     /gene="pstB"
                     /locus_tag="ACG9BB_000308"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFN2836286.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22171..22848
                     /gene="phoU"
                     /locus_tag="ACG9BB_000309"
     CDS             22171..22848
                     /gene="phoU"
                     /locus_tag="ACG9BB_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFN2836287.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23425..24117
                     /locus_tag="ACG9BB_000310"
     CDS             23425..24117
                     /locus_tag="ACG9BB_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFN2836288.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            24114..24881
                     /locus_tag="ACG9BB_000311"
     CDS             24114..24881
                     /locus_tag="ACG9BB_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFN2836289.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24981..26549
                     /gene="liaX"
                     /locus_tag="ACG9BB_000312"
     CDS             24981..26549
                     /gene="liaX"
                     /locus_tag="ACG9BB_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFN2836290.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26846..27166
                     /locus_tag="ACG9BB_000313"
     CDS             26846..27166
                     /locus_tag="ACG9BB_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFN2836291.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27166..27516
                     /locus_tag="ACG9BB_000314"
     CDS             27166..27516
                     /locus_tag="ACG9BB_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFN2836292.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27683..28621
                     /gene="hprK"
                     /locus_tag="ACG9BB_000315"
     CDS             27683..28621
                     /gene="hprK"
                     /locus_tag="ACG9BB_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFN2836293.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28636..29466
                     /gene="lgt"
                     /locus_tag="ACG9BB_000316"
     CDS             28636..29466
                     /gene="lgt"
                     /locus_tag="ACG9BB_000316"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFN2836294.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29510..30535
                     /locus_tag="ACG9BB_000317"
     CDS             29510..30535
                     /locus_tag="ACG9BB_000317"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFN2836295.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30550..31488
                     /gene="galU"
                     /locus_tag="ACG9BB_000318"
     CDS             30550..31488
                     /gene="galU"
                     /locus_tag="ACG9BB_000318"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFN2836296.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31613..32053
                     /locus_tag="ACG9BB_000319"
     CDS             31613..32053
                     /locus_tag="ACG9BB_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFN2836297.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32056..32658
                     /locus_tag="ACG9BB_000320"
     CDS             32056..32658
                     /locus_tag="ACG9BB_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFN2836298.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32717..33823)
                     /locus_tag="ACG9BB_000321"
     CDS             complement(32717..33823)
                     /locus_tag="ACG9BB_000321"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFN2836299.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34033..35034
                     /gene="ccpA"
                     /locus_tag="ACG9BB_000322"
     CDS             34033..35034
                     /gene="ccpA"
                     /locus_tag="ACG9BB_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFN2836300.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35371..37716)
                     /locus_tag="ACG9BB_000323"
     CDS             complement(35371..37716)
                     /locus_tag="ACG9BB_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFN2836301.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37995..38897
                     /locus_tag="ACG9BB_000324"
     CDS             37995..38897
                     /locus_tag="ACG9BB_000324"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFN2836302.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            39110..40885
                     /locus_tag="ACG9BB_000325"
     CDS             39110..40885
                     /locus_tag="ACG9BB_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFN2836303.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40951..42537)
                     /locus_tag="ACG9BB_000326"
     CDS             complement(40951..42537)
                     /locus_tag="ACG9BB_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFN2836304.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            42788..43012
                     /locus_tag="ACG9BB_000327"
     CDS             42788..43012
                     /locus_tag="ACG9BB_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFN2836305.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43153..44904
                     /locus_tag="ACG9BB_000328"
     CDS             43153..44904
                     /locus_tag="ACG9BB_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFN2836306.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44904..46688
                     /locus_tag="ACG9BB_000329"
     CDS             44904..46688
                     /locus_tag="ACG9BB_000329"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2836307.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            47014..47865
                     /gene="licT"
                     /locus_tag="ACG9BB_000330"
     CDS             47014..47865
                     /gene="licT"
                     /locus_tag="ACG9BB_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFN2836308.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47961..49841
                     /locus_tag="ACG9BB_000331"
     CDS             47961..49841
                     /locus_tag="ACG9BB_000331"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN2836309.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(49881..50783)
                     /locus_tag="ACG9BB_000332"
     CDS             complement(49881..50783)
                     /locus_tag="ACG9BB_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN2836310.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51014..52417
                     /locus_tag="ACG9BB_000333"
     CDS             51014..52417
                     /locus_tag="ACG9BB_000333"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFN2836311.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52417..53850
                     /locus_tag="ACG9BB_000334"
     CDS             52417..53850
                     /locus_tag="ACG9BB_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFN2836312.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            53995..54921
                     /gene="trxB"
                     /locus_tag="ACG9BB_000335"
     CDS             53995..54921
                     /gene="trxB"
                     /locus_tag="ACG9BB_000335"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFN2836313.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55135..56862
                     /locus_tag="ACG9BB_000336"
     CDS             55135..56862
                     /locus_tag="ACG9BB_000336"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFN2836314.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56877..57227
                     /locus_tag="ACG9BB_000337"
     CDS             56877..57227
                     /locus_tag="ACG9BB_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFN2836315.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
BASE COUNT        18569 a         9957 c        12351 g        16367 t
ORIGIN      
        1 agggactgcg acaagacttt tgtctcagtc cctttttttg catattttta gcatttattt
       61 cctaaaaaat gtaaggggat tcctatattt ttaagtatac ttgtttttcc ttttatacat
      121 tttatggtat tatcatttta ttagttatta taaaacaatc gttagaaagg acgtcattat
      181 gcaagtatac ggaattgatg cggtgcttcg aatcgtgagt catttggcgt ttatctactt
      241 agccttctgg tctttacgtt cgttacgcat tgaaactttt ttcagaccaa tgcataatac
      301 gcaaattaga atggcaatcg tacttttttc aattttttta gggtacactg caagtaattt
      361 tttcttagaa gttatcgctt tatgccgaaa tcttttcgtt agcctgtaaa ttttaaaaaa
      421 aggtactatt ttaaaagcaa ggatgttata atagaaaaga ttttgagtaa gtgtatgctc
      481 attggaattt tttggaggga acaacatgga agagatcatc gtaagaggtg gcaatcaatt
      541 aaatggaaca gtaagaattg aaggagctaa aaatgctgtt ttgcctattt tagctgctag
      601 tttgttagca gaagaaggta ttacgacatt agacaacgtt ccgattcttt ctgatgtctt
      661 cactatgaat caagtgattc gtcacttaaa cgtagatgta gattttgatg aacaaaagaa
      721 tcaagtaaca atcgacgctt caagacaatt agaaatcgaa gcgccttatg agtatgttag
      781 tcaaatgcgt gcttcaatcg ttgtgatggg tccgctactt gccagaaatg gacatgcaaa
      841 agtagctatg cctggtggat gtgcgattgg taaacgacca atcgatttgc atttaaaagg
      901 atttcaagct ttaggagcaa aaattattca aaaaaacggc tatatcgaag caattgcgga
      961 tgaattgatc ggtaacacga tttatttaga tttccctagt gtaggtgcta ctcaaaatat
     1021 catgatggct gccgtaaaag caaaaggaac aaccatcatt gaaaacgtag ctcgcgaacc
     1081 agaaatcgtt gatttagcaa acattttgaa taaaatgggc gctcaagtct atggagctgg
     1141 tactgaaaca atgcgtattg aaggcgtgga tcatttacat gcagtcaacc actcaatcgt
     1201 acaagatcgt atcgaagcag gtacctttat ggttgctgct gcaatgacac aaggcaatgt
     1261 gctgattgca gatgctatct ctgaacataa ccgcccattg atttctaaat tgatcgaaat
     1321 gggtgcagaa atcattgaag aagaaggcgg agtccgtgtc atcggaccaa aacatatctt
     1381 acctacagat gtaaaaacga tgcctcatcc tggtttccct acagatatgc aagctcaaat
     1441 gacagctatc caattagttg cagaaggtac aagtgtagtg actgaaacag tgtttgaaaa
     1501 tcgtttccaa catttagaag agatgcgccg tatgaacgct catgtcaaaa tcgacggaaa
     1561 tgtagcaatc atggatggaa accatgaatt gcaaggtgca gaagtatatg caacagactt
     1621 gcgggcagca gcagcattag ttttagctgg cttgaaagca aatggaatca cacgtgtgcg
     1681 taacttgaat tatctagacc gtggatatta taatttccat atcaaactac agcaattagg
     1741 tgcagacgtt gaacgtgtag acatggatca aacttctgct gaaaaaacag cacaaacgat
     1801 tgcttaagga agtggctgaa tgagtaacca acgatatatc ttattaactt tgataaagat
     1861 tttaatggtg atcttattgc ttatccttct ttttgtggcc ggaacgatga tcggatatgg
     1921 tgtcatcggt ggcggcaatc catttaaagt atttcagcca agtttgtgga ttcatattcg
     1981 agactttttt cattgataaa taagaatcac agtggttcta gtcgatatga agtgagccat
     2041 gaagcagaac atgataaaag aaaacagaag ggaacgttga cgttagtcgc gatccctttt
     2101 tttagtaaaa gaaaagaagg aatctaattg atgaagaaga tattgattta tttagttaga
     2161 ggatatcagc gctttatttc gcctgcattt ccacctagct gccgttatta cccaacctgt
     2221 tccaattata tgataaaagc aattcaaacc catggcgcgc taaaagggac ggtaatgggg
     2281 actgcacgga tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta tgtaccattg
     2341 acattccgat taacgcgaaa taagaaagat aaaaaagaac cagattataa aaaggttgtg
     2401 aaaaatacgg gtagcactga gcgataaaga aacaatttgg aaaatagctt cttatatttt
     2461 gcaaaattgt gagttattgc cgaagtgttg gattttgaac accgtcaaaa aaaaggttgt
     2521 gaaaaatacg gctagcactg agcgataaag aaaaagagga aatttattgt caaagtaaca
     2581 actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca agaaagattt
     2641 accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag tatatgtttc
     2701 cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac tgatctatgt
     2761 ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta accccaacgt atcaaggaaa
     2821 ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacaac tcgcttccag
     2881 tcaaactcag atcatcatac gaaccggcag tactagcagt gcggcgttac atctctatca
     2941 aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc attactctga
     3001 gcccattttt gaaaatggag atcaattaag ggatcaagtg attttagcaa ttcacttacc
     3061 taaaaatagc tttaactaat aaaatttcaa ttcaagaaaa tgctttcaaa tgctaaatag
     3121 atacattaca atagctttag caaacaaaaa ggagaatatt tttatgggaa tattagtttt
     3181 tgattttggt ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga ttaaaggtaa
     3241 gggacaattt gcaacgcctg aatcttggga agaaatgaaa gcgcaattat ttcaagttta
     3301 taaaaaaata ctcgtttctt ttgaagtcga aggaattgga atcagctctc ctggagtagt
     3361 gaataatgag aaacaggtga ttgagggaat cagtgcgatt ccttatattc atggatttaa
     3421 tattttccga gatttggaaa ctctgtttca acttccagta acgattgaaa atgacgccaa
     3481 ctgtgccgga atggctgaat tttatgaagg tgctgcccgc gattatcaag atgttgcttt
     3541 tgttgtggca ggaactggga ttggcggggc gttgttcacg aaaggacaaa tcaataaagg
     3601 ggcacatctt tacggtggag aatttgggtt gatgttctta aatggcgata agacattcag
     3661 taaacttggt acagcggtac agatggcttg gcgttattgt gaacgcaaag gtctcgataa
     3721 aaatgcttat accggaaaag atgtctttga attagcagaa actggagatg caatagcaaa
     3781 agaagaagtt gagagttttt atacgtatct tacaaaggga ttattcagta ttcaattttc
     3841 gtttgatcca gaagtgatcg tattaggcgg cggggtttct gcaaaagaag ggctcattga
     3901 tgagatcaaa agccgaatga agaagctgac agaacaattc gatctgcatg atttcgaacc
     3961 gcagatttta ttatgtgaat acagaaacga tgctaatctt gtaggtgcag cagcaaacta
     4021 tctcgctgta cgtcaagggg agaattaaga gctttagaga aggtgcagct tgcataaaag
     4081 aagtgaatga aatgaatagg agatttttac ctaaggaatg ttttatttgc tgcagtatga
     4141 taaaatatct gagaggtgaa aatgatgtca aaaaaaaata aagatattga agtgcgtata
     4201 gaagaagtga aaaaagatat caaaggtaag aattatgaag tcactcagtt atttataggg
     4261 aaaaaaatga ttggtgagat cttagcctat ggaccaaaag aatatgaaat tttctttggt
     4321 gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt gattcgacac
     4381 tggaatctgc acgaataaaa aaatatcaaa aaaactttca ataaagggtt gcaaaaatct
     4441 ggaaaattag gtattataac taagtcaggt tgatacaaac ctttcggagg agtagcgaag
     4501 tggctaaacg cgacggactg taaatccgtt ccttagggtt cagtggttcg aatccactct
     4561 cctccatttc attggggtat agccaagcgg taaggcaagg gactttgact ccctcatgcg
     4621 ttggttcgaa tccagctacc ccagtatacc acaatcgtaa gctctcgtta gttcgagagc
     4681 tctttttttt gctttgctac cttgttcctt gaagattctg ctgatttaag atagagttaa
     4741 gttaactaca agaattggag ggatccacat gaaatggaaa gaaaaagtag acgaagcagc
     4801 tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat attgaagtga atattccgaa
     4861 aaaaggcgga aaagcggtcc gggtaaaatc aaaacgccca acgaatcata caaaaaaatg
     4921 gatggctaaa aaccgctaat aataacaaaa gatccataag tcggcatttt catcgatttt
     4981 agggtctttt gttattttct aggttggaca tatctaaaaa attgtttctg acaataggag
     5041 taggaagaac gatgtgtgtc taaatcctct gttgatggat agaaaccttt atctttttca
     5101 gttgtgaatc aggtatatac cagttataat agaagaaacg aaaggacggg tgtacgttat
     5161 gtcgaatcaa ttacctgtat atatacagat tcatgaccaa ttaaaaaatg aaatcgaaca
     5221 aggtatatgg aaagtcggag accgtcttcc ttcagaaaga gaattagcag tgaaattcaa
     5281 tgtcagccgt atgacgttga gacaagcaat tcaaaacttg gcagatgaag gaatcttaga
     5341 aagaaagatc ggttcgggta catatgttgc acgagaaaaa gtacaagaaa aaatgtctgg
     5401 tgcaacaagt tttacagaaa ttatggaatc acaaaatcga gtaccgtcta gtcggacaat
     5461 ctcttatttc ttgacttcgc caagctcaag tgaaatggaa aaactaaact tgagtaaaga
     5521 tgacacgatc ttgcgaatgg aacgtattcg tttcgcagat gatgtcccta tttgttttga
     5581 agttgccagt gtgccacaaa agctgatcga tggctacagc aaagcagaga ttactgaatc
     5641 gttgtatcgc acgctagaag aaaaaggcgg gaaaaagatc ggtccagcga atcaaacgat
     5701 tactgctgtg ttggcttctg agaaaattgc tgactattta gaggtaaaac gaggagatgc
     5761 agttcttcgt ttgcgccaga tttcttattt tgaagacggt acgccgtttg agtatgtgcg
     5821 tacgcaatac gtaggaaatc gttttgagtt ttatttggaa aaataaaaaa caggtgatcc
     5881 ctgattccgt attagaaata aaagagaatc agaatcacct gtttttatat atgatcagtt
     5941 accagttgct cgtgataaga aacaggaccc tcttcaaatt ggacttgtcc gttcagcagc
     6001 tcaatgatgg attctttaaa gtggtcagca cttttttcat ctacagcaca gatgacgcta
     6061 acttgatcag tatattggat ttcttttata aaaaaatgtt ggagttcaag ataattctgt
     6121 aattttccta gttgcggata cgtgatcatc acagagatct cctgttgcaa ggtaccttct
     6181 acgattccaa tgtcagacaa tgcttgagag accgcatgag tgtatgctcg tattaatccg
     6241 cctgtgccga gtttcgttcc tccaaaatat cgagtgacta ctgcagctac attgatcagt
     6301 tgattttttt tgaggacttc caacatcggt atgcccgccg ttccacttgg ttctccatca
     6361 tcgctgcttc gctggatttc atttttttct ccaatgatga aggcgctgca gttatggttc
     6421 gctttccaat gttctttttt tatttcttga atgaaacttt ttgcttcgtc ctctgattct
     6481 attcgtctaa gagaacagat aaagcgtgat tttttgatct cgatctcact ttgtccattt
     6541 tcttttattg tacgatagcg ctcgatcatc ttgtcacctc ttttttcatt ttctttattc
     6601 agtataaata aagttctagg ttgtttcaac actgtaacgg ttgcgcaatt gctgtagtat
     6661 gcgataataa agacaaaggc aggtggatct ggggatgcag gaacgaatga aaaagtacga
     6721 tgcaattacg cattatttga agaacaatgg cggctctcaa gtaacgttga cctttaccca
     6781 atttgacgaa ctgctgtttc ccagtaacgg acttcccaaa acagcaaggg aaagtacaga
     6841 ttggtgggca aatgattata aacaccctga aaaaggtgcc tatggctgga taaatgcagg
     6901 ctatgaagta gttgtcatca atttggacaa agaatatgtc gtgttcaata aattagtgaa
     6961 atctagttgg ctatttgatt aaataaaccg cgtttagaaa acgttgacaa atttctttct
     7021 ttttatataa tagagtaaca gacgcgaaga aaacgtttgc gtagtttgtt ttaaaaagga
     7081 gagaaaagta tggctaaaaa aacaatcatg cttgtatgtt cggctggtat gagcacaagt
     7141 ttactagtaa caaaaatgca aaaagctgct gaagcaaaag gaatcgactc tgatattttc
     7201 gctgtgtccg cttctgacgc agataacaat cttgcaaata aagacgtgga tgtgttgtta
     7261 ttaggcccac aagttcgctt tatgaaagct gattttgaga aacgtctgga accaaaaggc
     7321 attcctttag atgtcatcaa tatggctgat tatggtatga tgaatggcga aaaagtgtta
     7381 gatcaagcaa tcacgctcat aggtgaataa atagaacagt ataactatcg aacaagcgat
     7441 ccaaaaaata gatcgcttgt tttttcgtat atttttttag ggcgaataat atgaatcatt
     7501 tttggggtag aaggcttctg acgagagata ttaaagcaat agactgtgag gaggtaaatt
     7561 taccaaatta tgagaagatc gcagcagtgg aacgtttgag caactggatt ctttgtcatc
     7621 gctgtggaat gagaacgtcc gtcattgatg gacagcttgc agattacggt tatttttgta
     7681 cacattgtct atatttaggg cgatgcgaca ctcaacaaga tttatatttg tttgatcagc
     7741 ctgaagcaaa acctcgagaa gtcgtctttt tttggacagg acagcttact gaaaaacaaa
     7801 cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt atttgggctg
     7861 taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact ttaaaagctg
     7921 gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg tttttacgct
     7981 atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca gcaacggctt
     8041 atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt tatcggtcct
     8101 ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat gcaggattag
     8161 ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta agtgccacac
     8221 ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa cttgctttac
     8281 gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat tggcgacaaa
     8341 aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa ttaattaaag
     8401 aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg aaagccacac
     8461 ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac aatcagcggg
     8521 cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc acgatgatcc
     8581 ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg gatcatccgg
     8641 tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa gggccttata
     8701 cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga aaagcttgtc
     8761 aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca gttgctgcag
     8821 taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga aatacagatt
     8881 acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa gaggctgttc
     8941 tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag taaattctat
     9001 ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct atgattttca
     9061 gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc aatacaagtt
     9121 tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga taaaaaaaga
     9181 atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa cagaaagagg
     9241 attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga tgctactcaa
     9301 aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat tggagatgcc
     9361 tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa tattgattat
     9421 tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt tgcagatcgc
     9481 acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg gaaatttgat
     9541 agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa gagggaaaga
     9601 gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg tttagatata
     9661 atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt gagaaaaaag
     9721 taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat gtgaacttaa
     9781 aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct tatcttgtat
     9841 tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt gttgacaaat
     9901 tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt gaaacgggca
     9961 tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa aatgattctg
    10021 aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt gaagaagcag
    10081 tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca gaaacaaatg
    10141 gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa acagactgat
    10201 cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc cgcaagtttt
    10261 tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct tactttaata
    10321 cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa gtctacatag
    10381 tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt tgattcgttc
    10441 acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa aaaaagttga
    10501 agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa aaactgctga
    10561 attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg aagcttttgc
    10621 agtcgtccga gaagcagcaa aacgtgtatt aggtttatat ccataccacg tacaattgat
    10681 gggtggaatc gtgcttcacg atggaaatat ccctgaaatg cgtactggtg aaggtaaaac
    10741 cttgaccgca acgatgccag tatatctaaa tgctctgact ggcgaagggg tacacgtagt
    10801 taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgaac tttataattt
    10861 cttaggatta acagtggggt tgaatatcaa ctcaaaatct tcggatgaaa aacgcgaggc
    10921 ttataattgt gatatcactt atagtacgaa taatgaatta ggatttgact acttgagaga
    10981 caatatggtc gtttaccgcc atcaaatggt acaacgtccg ctgaattatg ccattgtcga
    11041 tgaagttgac tcaatcttga ttgatgaagc gagaacgccg ctgatcattt cagggcaagc
    11101 agaaaaatcc acagctttat atacaagagc agacaacttc gtgaaacgtt tgaaagaaga
    11161 agaagattat aaaatcgatg tacaatcaaa aacaatcagt ttaaccgaag cagggatcga
    11221 aaaagcagaa gaaacattcg gtttggataa cttatacgac attgaaaata ctgcattgac
    11281 ccatcacttg gatcaagcat tgagagccaa ctatatcatg cttttagata tcgattatgt
    11341 tgtccaagac ggaaaagtac tgattgttga ccagttcact ggtcggatca tggatggacg
    11401 ccgttattca gatggacttc accaagcaat cgaagctaaa gaaggcgtag agatcgaaga
    11461 cgaaacgaaa accatggcga cgatcacttt ccagaactat ttccgtatgt acaagaaact
    11521 tgcgggaatg actggtacag ctaaaacaga agaagaagaa ttcagagaaa tttataacat
    11581 ccaagtatac cagattccga caaatcgtcc ggttatccgt gatgatcgtc cagatttgct
    11641 ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa gatatcaaag atcgctaccg
    11701 caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa acatctgagt tattatcaga
    11761 tatgctgaat caagaacgta ttcctcatga agtgctgaat gcgaaaaatc actttaaaga
    11821 agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg acgattgcta ccaacatggc
    11881 tggtcgggga acagatatca agttaggact tggggtaaga gaacttggtg gtctagcagt
    11941 tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag gacgtgcagg
    12001 acgtcaagga gaccctggta tgtcacagtt ctacttgtca ttggaagatg atttgatgaa
    12061 acgattcggt tcagaacgaa tcaaatcttt cttagatcgg atgaagatcg gtgaggaaga
    12121 tgctgttatc caaagcaaga tgctgacgaa acaagtagaa tctgcacaaa aacgtgtaga
    12181 aggaaataac tatgacacac gtaaaaacgt cttacaatac gatgatgtga tgcgtgaaca
    12241 acgtgaagta atctataaac agcgacaaga agtaatcatg gaagaaaaaa gcttatccaa
    12301 gccactaatg aacatggtca aacggacaat cagtcgtgta gtagatgcgc atacacaatt
    12361 agagaaagaa aattggaatt tggaaagtat catcgatttt gccggcagta ctcttgtcca
    12421 tgaagatagt attactcttg cagatatcga aggaaaaaca gctcaggaga tcaaagagta
    12481 tctaaacgaa cgagcacaag aagtatttaa taccaaagcg gctcaactaa atggtccaga
    12541 gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt gtggactcta aatggacaga
    12601 ccatattgat gcaatggatc aattacgtca gtcaatcggc ttacgtgctt atggacagaa
    12661 caatccatta gtcgaatatc aaacagaagg ctataagatg tttgaagata tggtaggcgc
    12721 catcgagtat gaagtcactc gaatctttat gaaagctgag atccgtcaaa acgtacagcg
    12781 tgaacaggtt tctcaaggaa cagcttctca ctcagaagac ggagatgcca aagaaaatag
    12841 taatactagc gcgaaaaaac agccaatcca tgtgaacaag gtcggaagaa atgatccttg
    12901 tccttgtgga agcgggaaaa aatataagaa ctgtcatgga aaaaatgcaa actaatttct
    12961 gacagcagga ttggtgagac aaattctttt tttaagaatt cgtctcacct tctttctgtt
    13021 atttgctgat tctgttagac tagatataat gaaaaaagta tagtgaaaga aggaaaaaac
    13081 atggaaatat ccgaaattcg aaatctcctg acagaaatgc aggaaaaggt cacaagcttc
    13141 aggaggtctc tttgacttag accagctgga agaagacatt gcggaagcag aagcaaaaat
    13201 ggctgaacct ggtttttggg atgatagcga agctgctcaa aaagtgatca gtgaaaacaa
    13261 tgcaaataaa gaaacttatg atcaattcca ttctcttgct gaagaactgg aagaaatcga
    13321 attgatggtg gagatgttgc aggaagaacc tgatgcagcc atgcaagaag aagcagaaac
    13381 acgtatcaat gcgctagatg aaaaaatgaa gacatatgaa ttatctatgt tgttagatgg
    13441 tccttatgat cgaaacaacg ccatcgttga attgcatcca ggggctggcg gaacggagtc
    13501 ccaagattgg ggcagtatgc ttttacgtat gtatacacgc tgggcagagc agcatgggta
    13561 tcaagtagag acactagatt atcaagcagg tgatgaagca ggaatcaaga gtgtgaccct
    13621 actgatcaaa ggacacaacg catttggtta tctgaaatca gaaaaaggtg ttcatcgcct
    13681 tgtacgtatc tcgccttttg actcagcgaa acgacgtcat acttcttttt gttcggtaga
    13741 tgtgatgcca gagcttgatg aaacgatcga tatagaaatc aatccagatg atttgaaaat
    13801 cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc aataaaacag aatctgctgt
    13861 acggatcact cacttgccta caggaacagt agtagctagt caggcacaac gttcacaatt
    13921 gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag ctttatcaat tagaagtgga
    13981 aaagaaagaa caagaagcag ctgcacttcg aggagaacaa aaagaaatcg gctggggttc
    14041 acagatccgt tcttatgttt tccatccata ttctatggta aaagaccatc ggacaaacgt
    14101 agagacagga aatgtccaag cagttatgga cggtgacctt gatttattta ttgacggcta
    14161 tttaaaaatg catctacaat aagaacccat tgttaaattg aaataaaatt gtaagatttt
    14221 gcaagcatgt tgaaacattg ccatgctata atagctaaag actgaaaaag atatggagag
    14281 aacgccatga ttgaaatgaa ggatgtaatg aagaagtact caaatggtac gaccgctatc
    14341 cgcaatctct caattgtcat cgaacaagga gagtttgtct atgtggttgg tccgtctgga
    14401 gccggtaaat ctacattcat caaattgatg tatcgagaag aaaaagcttc aaaaggaact
    14461 ttgaacgtcg caggtcatga tctaatagca atcaaaaacc gcgaagttcc ttatctacgc
    14521 agagaaatcg gcgtggtgtt ccaagactat aaattattac caagaaaaac agtatatgaa
    14581 aatgttgcgt acgctatgca agttatcggc agaaaacctc gggatatcaa aaaacgagta
    14641 atggaagtgc tggaccttgt tggtttgaaa cacaaagttc gagtctttcc tagcgaatta
    14701 tccggtgggg aacagcaacg tgtctctatt gctcgggcaa tagtgaatac gcctaaagta
    14761 cttatcgcgg atgaaccgac aggaaatctg gatccagaaa actcatggga aatcatgaaa
    14821 ttattagatc ggatcaatgc ccaaggaact actgtcgtga tggctacaca taacagtacg
    14881 atcgtaaata cgattcggca ccgagtaatt gctatcgaaa acggccgcat tattcgagat
    14941 caagcggaag gggaatatgg atacgatgat tagaaccttt ttccgccatt tattagaaag
    15001 tatcaagagt ttgaaacgta acggctggat gacagttgct tcagtcagtg cggtaacgat
    15061 cacattgact ttagtcggtg tattcatggc agttatcatg aacgcaacta aattagctca
    15121 agatatagaa ggaaacgttg atgtctctgt attcgtagat atcggtacaa aagacgaaga
    15181 gatgaaaaag ctagaaaaag aattgaatga cttagatcat gttaaaaaag ttgaattttc
    15241 aagcaaagaa caggaattga aaaaaatcca aaacgaaatg ggtgattcct ggaaattgtt
    15301 tgaaggtgac aataacccgt tatatgatgt gtatgtagta agtgctactg atccttctta
    15361 tacaaaacaa gtagcagaag aagcagcaga tttgaagaat gtttctcgtt ctgattacgg
    15421 tggagcttcc tcagatcgga ttttccaaat cgctggtgca gttcggacat ggggattagt
    15481 tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt tcgaacacga ttcggatcac
    15541 gattttgtct agacagcgtg aaattcaaat catgcgttta gtcggtgcaa aaaatggata
    15601 tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt ctgatcggtg cgattcttcc
    15661 gattttgatc atcacatttg gttatgcatg gtttttccaa ttgatcaatc catcattatt
    15721 acgttcccat tattcattga ttcatccaca aaatatcgtt tggaaaatca atcttttaat
    15781 ggtcggcatt ggtgtgatca ttggttcact tggctcgatc atttctatga gacgtttctt
    15841 gaaattctaa taccacaata taaataaaat gagaaagttg gacagtttcg ttccagcttt
    15901 ctttttttat tggctaggag aatagaagga agcggctttt ttccctttac tttaaaaaac
    15961 aacttgggta ttcttaagtt ggtagaagaa gaggtgaagc cgtgaaaatt atcgttgaca
    16021 atttttttac gatccttttg attatgaata ttttgctttc ctttatcatc gtttttcgag
    16081 aaagaaagga aactgctcaa acatgggcat gggtgctcgt tttgatgttc attccagttg
    16141 taggatttat tttatatatt tttttaggaa gagggatttc caaagagaaa atctttgatt
    16201 tgaagatcca agggaaaatc ggtaagaacc tggaaattga agaagacaaa caggcattga
    16261 tgcgagggct ttatcctcat ccgcctactg gacaagtaga tgtgaaacaa ttgatctata
    16321 tgctcacggt atttgaaagt acgctctata cgactaaaaa tgaaatcact ttatttacag
    16381 atggtcgtga aaaattcgat gcgctgattg aagatatcaa gcaggcagaa gaccatatcc
    16441 acttccagta ttatatttat cgcagtgatg cattaggtga agaagttcga gatgcattga
    16501 cagaggcggc aagacgaggg gtaaaagtca gagtccttct tgatgcatgg ggttccacac
    16561 aagtttcttc cagctttttt cagaatttga aaaaagcagg cggagaagtc gcatttttct
    16621 ttcctctatt tgttccgtat attaatcctc ggatcaatta tcgcaatcat cgtaaaatcg
    16681 tagttattga tgggaaaatc ggatatacag gtgggttcaa tgttggaaat gaatatctag
    16741 gattggtaaa gaaatttggt tattggcgag acaatcattt gcgtatttac ggagaagcgg
    16801 tgtatacctt gcaaaaccgt tttttgatgg actggaattc tcagcataca aaagaagtag
    16861 gctattctcc caaatttttt ccgtccattc actctactgg tgatatagct gttcaaatcg
    16921 tgacaagcgg accagatacc gaacatgaac aaatcaaaat gacttatttg aaaatgattt
    16981 cgttagccaa acgggagata ttgatccaaa cgccttatta cataccggat ggatcgattc
    17041 atgaagcact gaagttggct cttctttcag gcgtaaaagt tcatatccaa atacctaaca
    17101 aaccagatca tcttctcgtt tactgggcaa cgtattcttt cgctgcagag ctaatcgagt
    17161 atggagcaag gatcgaaaca tatgagaatg gatttattca tgcaaagacg atgattatcg
    17221 atggtggcat tgtttcagtt ggctcagcaa atatcgatgt tcgttcattt cgcttggact
    17281 ttgaggtcaa tacactggtt tatgatgaac gaatggcttc acgtgtaaga aaagcttttt
    17341 ttgaagactc gaaaatttct acgcatctca caaaagaaat gtatgaaaac cgaggaataa
    17401 tcatcaagat gaaagaagga ttagcaagat tgatttcgcc gctcctttaa aacataaaaa
    17461 ggacatgaga accatcaaca gatggagatc tcgtgtcctt ttctaacggg gccatttctg
    17521 agagtacgtc agaaatggag aaagcgtctc gcatgagcga aattaacttt ggggtatctc
    17581 ttgttttttg gggaaagcaa gaaataagct aatatatgct attaaaagag acacatttca
    17641 aagccagtat agcatagaaa cttttaatga gatagttttt tttacaatta tgagaattta
    17701 ttttgttatt taatgatttt gattcgtttt catcaagtaa tgaaaatgat aaaataaaca
    17761 tactttagga ttaaaggaga tttctaatga aaaaacttaa attattctta ccaataatag
    17821 gattagctat tcttttatca ggctgttcaa agtggattga tcgaggagaa tccattacgg
    17881 ctgttggctc ttctgcttta cagcctttag tagaaacagt tgctgaaact taccagacag
    17941 aaaatcctgg aaaattcatc aatgtacaag ggggcggaag cggaactggt ttgtctcaag
    18001 tacagtcagg agcagtcgac atcggaaact ctgatttgtt tgcggaagaa aaatcgggga
    18061 tcgatgcctc agcattagtc gatcatcgag tagcagttgt tgggatcact cctattgtga
    18121 acaaggatat tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta tttacaggcg
    18181 agatcaaaaa ctggagtgaa gtcggtggaa aagatcagcc agtagttata ttgaaccgcg
    18241 cttcaggaag cggaacccgt agcacgtttg aaaaatgggt attggacgga gaaacagctg
    18301 ttcaggcaca agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc gacacacccg
    18361 gtgcaatcag ctatgttgct ttttcttatg tttccgatga tgtagatacg ttgaatgtta
    18421 acggtgtaga accgacagat gaaaatgtga caacgaacaa atggacgatt tgggcatatg
    18481 agcatatgta cacaagagat catccaaaaa aactgacaaa agaattttta gactatatct
    18541 tatctgatga agtacaagat aacatcgttg gagaactagg ttatatccct gtttctcata
    18601 tgaaagtcga acgtgattgg caaggaaata tcgttaaaga gtaatcttta cacaaaattt
    18661 acaaacttag ctaaactttt acatgttatt aaaaccaaat tgatgttcaa tttgtacact
    18721 gagtgagtat gttaaacctg attaaggggg agtcatctgt ggaagatgtg aaaaaagtat
    18781 tattaacaaa atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc agttttttat
    18841 gtatcgcgct gatcgtgttg gttgtattat ctatttttta ttttgtagct agtaaaggat
    18901 tggccacctt ttttactgat aagatcaatg tatttgattt tctgtttggt acagattgga
    18961 atccaagcca aatcggagca aatgggaaac caatggtagg ggcattgcct atgatcacag
    19021 gttcgtttat tgtgaccttt ttatctgcta tcgttgccac gccatttgcc attggtgcag
    19081 cagtattcat gacggagatc tctcctaaaa acggaacgaa gatcttacag ccggtcatcg
    19141 agctgcttgt aggtattcct tctgtcgttt atggttttat cggtttatca gttatcgtgc
    19201 cttttgtcag aagtattttt ggcgggactg ggtttggtat cttagctggt acatttgtgt
    19261 tgttcgttat gatcttgccg actatcacaa cgatgactgt tgatgcatta aaggcagtgc
    19321 cacggcacta tcgggaagct tcattagcgc ttggtgccac aagatggcaa acaatctaca
    19381 aagtcgtgct acgagcagcc gtgcccggga ttttaacagc agtcgttttt ggtatggctc
    19441 gtgcgtttgg tgaagcctta gctatccaaa tggtcatcgg taatgcagca ttgatgccaa
    19501 caggtcttac tactcctgct tctacattga cgtccatctt aacaatggga atcggaaata
    19561 caatcatggg aacagttgaa aataatgtgt tatggtcatt agcgctgatc ttgctattga
    19621 tgtcattatt ctttaatatc gtgatccgta tgattgggaa gaaaggagag ctgaaataat
    19681 gaacgcaaaa cgctcagata aaattgctac aggtgtttta tatatagttt cggggatcat
    19741 cgtattgatt ttagcagctt tactacttta tatcttagtt cggggaatac cgcacatttc
    19801 atggtccttc ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa tcgggatcca
    19861 gctatttaac tcgatttatt tgcttttgat cacgatgatc atcagttttc ctatttcttt
    19921 aggtgcgggt atctatcttt cagaatatgc tggaaaaaac tggctaacag atgtgattcg
    19981 cacgtcgatt gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat tcggtttctt
    20041 ggttttcgtt attcaattcc agtatggttt ctcgattatt tctggtgcat tggctttgac
    20101 tgtattcaac ttgcctcttt tgactagaac agttgaggag tctttacaag cggtccatta
    20161 cacacaacga gaagcaggat tggccttagg tttgtcacgc tgggaaacag tgataaaagt
    20221 ggtggttcca gaagcgacac cagggatttt gactggtgtg atcctaagtt ctggaagaat
    20281 ttttggtgaa gcagctgcat tgatctatac ggctggacaa agtgcacctg cgttagattt
    20341 tacgaactgg aatccattaa gtgtttcaag cccgatcagc attttccgtc aagctgaaac
    20401 attagctgtc catatttgga agatcaatac agaaggcacg atgcctgatg gtgtttctgt
    20461 ttcggctggc gcttctgccg tgttgattat tgctgtttta ctgttcaact tcggcgctcg
    20521 tgcgattggc aaacgattat accgaaaaat gacttcagcc taaggagaag aagcaaaatg
    20581 aaaaaatata actggaatga aacaaatgtc atcaccatgc caaaagagaa agaagtagca
    20641 ctatatacgg atgatctaca tgtctggtat ggcgacaatg aagcaatcaa aggagtggat
    20701 ctggaatttg aaaaaaataa gatcactgca ctgattggtc cttccggctg cggaaaatct
    20761 acgtatcttc gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa aatcactggg
    20821 aaaatcatgt atcaaggcgt agatattaat gcgcctcaag tggatgttta tgagatgcgc
    20881 aaacggattg gcatggtttt tcaacgtcct aatccgttca gcaagtcgat ttatgacaat
    20941 attacatttg ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga tgaaatcgtt
    21001 gaaacaagct tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt ggacaaaagt
    21061 gccttggctt tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc gatcgcgatg
    21121 aaacctgata tcttgctgct agacgagccg gcaagtgctt tggaccctat ttcgacagga
    21181 acggttgaag aaacacttgt aaacttgaaa gaagactata cgatcatcat cgtgacgcat
    21241 aacatgcaac aagcagctcg tatcagcgac tatactgcct ttttctattt aggaaaagta
    21301 atggaatatg acaaaactaa caagatattt accagaccaa aaattcaggc aaccgaagat
    21361 tatgtgtccg gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga gattatttca
    21421 tctaaagatc ttcatctgta ttatggaaag aaagaagcac taaaagggat cgatctaagc
    21481 ttcaatcaag gggaaatcac tgcgatgatc ggtccttctg gttgcggaaa atccacttat
    21541 ctgcgttctt tgaaccggat gaatgacctg attccgaatg taacgatcac cggaagtgtt
    21601 ttatataaag acaaagacat ctatggtcca aaaacagatg ttgttgaact aagaaaagaa
    21661 atcggcatgg tattccagca gccaaaccca tttccttttt ctatctatga aaatgtcatc
    21721 tatggattga aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt agttgaagaa
    21781 agcctgaagg cagcgtctgt ttgggatgat gtgaaagaca aattgcataa aagtgcttta
    21841 tctttatccg gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc agttaatccg
    21901 gaaatcatct tattggatga accaacaagt gcgcttgatc ctgtttctac tgggaaaatc
    21961 gaaagtatgc tgttagaatt aagagagcgg tatacgatga tcatcgtgac ccataatatg
    22021 tctcaagctt cacgtatttc agataagacg gcattctttt tagatggtca cttgatcgaa
    22081 tacaatgata cgaagaagat attcatgaat ccagaaaaac aagagacaga agattatatt
    22141 tcaggtcgat ttggataagg aggaaacgga atgctacgta cacaatttga agaagaattg
    22201 ctgaatctcc ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc cgtgcacaag
    22261 tcagttcgtg catacatcaa acacgataaa atcttagccc aagaagttat cgaaaacgat
    22321 ataaatatca ataacatgga aacacgcttg gagaagaaaa gttttgaaat gatcgcattg
    22381 caacagccag ttacaactga tttgcgtatg atcattacgg tcatgaaagc tagctcggat
    22441 ttagaacgta tggccgatca tgcggtgtct gttgctaaat ccacgattcg cgtaaaaggg
    22501 cagacgagaa taccggaaat cgaaaaagag atctccgata tgtccgatta tgtgaaaaaa
    22561 atggtggaca acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc acgtacgatc
    22621 gccaatatgg atcaaagagt caatgactat ttcaatcgaa tctataatgc aacaatcaaa
    22681 aatatgcagg aaaatcctga gacagtcatc agtggaacag actacttgaa tgttgccagt
    22741 tatctagaac ggatcggaga ctatgtgaca aacatctgtg aatggatcgt ctacttggct
    22801 acagggaaaa tcactgagtt aaatacgaac cacaatgaga ttttctaaac tgaagctgtg
    22861 attatgccgt ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa aagctgttct
    22921 gcatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg
    22981 acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa
    23041 aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt
    23101 agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagcc
    23161 gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga ggatcatcaa
    23221 ttgattacag gaaaactgat gaacacggat caaaaaggag aatcgattta tttatcggtt
    23281 ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc
    23341 caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg gcttttcttc
    23401 ttcacaagta tcttcattgg cgccatgttg cttattggtg gaaaacaggg tctatttgct
    23461 ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa
    23521 acagaaggga tttctctatt aaatatatct atcctgttcg tcgttatagg gatgattatt
    23581 agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt
    23641 acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa
    23701 ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct
    23761 agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca
    23821 ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga
    23881 aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc
    23941 agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcgtt caaagaaacg
    24001 atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta
    24061 ttatcgatcc ctatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg
    24121 tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag
    24181 gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg
    24241 gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg
    24301 tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta
    24361 tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt ttagcaatcc
    24421 aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg
    24481 attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg
    24541 cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt
    24601 tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa
    24661 tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt
    24721 tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg
    24781 ctattcttac cacattgtcc gcactacttg ttcttccttt aacctgcgtg actacagcat
    24841 atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct
    24901 atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt
    24961 tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag
    25021 gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg
    25081 aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa aaagatcagg
    25141 caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc
    25201 caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa
    25261 ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata
    25321 aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag
    25381 gcgagttgac agaagaagaa ttagcacagc gtcagacgtt agaagcagaa atcaaagaat
    25441 tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag
    25501 atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc
    25561 cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag
    25621 aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag
    25681 tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga
    25741 aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag
    25801 caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga
    25861 tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga
    25921 ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc
    25981 ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca
    26041 acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca
    26101 tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg
    26161 atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat
    26221 ttggtgcgac agcagaaaac ctttctgtgt ctttggtaaa tggtgatgta cgtcttacga
    26281 ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg
    26341 ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaaca
    26401 gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc
    26461 aattccgtcg tgtttcagat ggtgaaatcg cgcaagtgca actttcaacg acaacaggaa
    26521 gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag
    26581 caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc
    26641 gatagtctat ctcttgaaca ccgtttacca agagattatg aacctgccaa ttatcgccga
    26701 gtcacaagga ataatttcag aaaaagctcg tcacgtttta tgaatatggt aatttgtgac
    26761 cgagaatttt gcagaaagac accgattaag aagaaggttg tcacaacgat tttcttcatt
    26821 taaaatagta gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat ctaataaaaa
    26881 tgttgttttg acagggactt tagcaggaat aggagaatac atcgggattg atccgactgt
    26941 tatccgtgtg ctttatgtat tcctgagctt agttgctttt ggaagtccaa ttattttata
    27001 cattttatta gctttgatta tcccatttgc gcgttctgca cgaacaaact atggacataa
    27061 caatgcatat tatgaaaaaa acaattatcg tgaacaaact cgtgaacaaa caaaaaaacg
    27121 caaagaagcg gaaaaaatcg atgatgacga atggagtgac ttttaatgtc gtactttcaa
    27181 cgtattttag ttaatacgtt aacattcata tcattggctg ttctttttcc tgcgatggtc
    27241 catgtgagca gtatttggac ggccgttctt gccagcttcg tattgtctgt gttgaatatg
    27301 tttgtcaaac cgattttgac gatactgtcc ctgcccttta cattagtcac attgggattg
    27361 tttacgtttg ttatcaatgc agcgattctt aaaatgacct ccttttttgt gggagcagaa
    27421 cattttagtt tttcttcatt cggtgcagcg ttgttagtag ctatcttgat gtcgcttgtc
    27481 aatgtaattg tcagcgaaca taacagtaga aaataaaaga aagctgtgcc gaactatcgg
    27541 cgcagttttt ttatttagaa tcaggaatgc gttttctatt acattggtaa ttaaataaaa
    27601 acgttttttt taattggtag tacttttggc tagacaatga taaaatgata gagaacactc
    27661 taatagaaat gaggtagcat gaatgtcaga agttgtaaaa gttagccaat tagtgaaaaa
    27721 attatctttg gaaatcgtga caggcgacga gaaaagctta gaccgtgtca tcgtaactgg
    27781 ggacatctca cgcccaggac tagagttgac tggttatttt aattattatt cccataatcg
    27841 tttacagcta tttggcagta aagaaatcag ttttgcggaa cggatgatcc cagaagagcg
    27901 tttgatcatc atgagaagat tatgtagtga agatatgccc gcatttatta tttcaagagg
    27961 attaccagct ccagaagaaa tgatccaggc agcaaataaa aatgggatag ctgtactgcg
    28021 ttctcccatc tcaacttctc gtttattagg agaattatcc agttatttag atggtcgcct
    28081 tgcccctcga acaagtgtcc acggtgtttt agtggatgtt tacggattag gtgtactgat
    28141 tcaaggtgac agtgggatag gaaaaagtga aacagcgttg gagttgatca aacgcggcca
    28201 tcgattgatc gcagacgatc gagtagatgt ttatcagcaa gatgaattaa cggtgatcgg
    28261 agaaccgcct aaaatcctgg aacatctgat tgaaatccga ggaatcggga tcattgatgt
    28321 aatgaatctt tttggtgcga gtgcggtacg tggctctatg caagtccagt tggctgttta
    28381 tctggaagca tgggcaaaag ataagaaata cgaccgtctg ggctctgaag acacgaccgt
    28441 tgaaatcgcg gaagtgggca ttccacaaat aaaaatccct gtcaaaacag gacgaaacgt
    28501 tgcaatcatc attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg gctttgacgc
    28561 aacgaaaaca tttgaagaaa gattaagtcg cttgattgaa gaaaactcag gcaacgacta
    28621 gcaaggagtg acaatatgtt agcacaaatc aatcgagttg cttttgagct tgtagggatt
    28681 cctatctact ggtatgcatt gatcatcgta tcaggtatca ttatcgctat gtggcttagc
    28741 agcagagagg ctgttcgggt aggattaaaa gcagatgatg tgacagattt tatgttgtgg
    28801 ggattgccta tctccattat tggcgctcgc ttatattatg ttttatttga tcttccccag
    28861 tatatagctg atccaatcca aatattcaat atccgttcag gaggcttagc tatttatggc
    28921 ggactgattg caggcgcgat cacgctgtat ttttttacta aatatcattt tatttctttg
    28981 tggacttttt tagatatcgc tgcaccaagt gtattattag cacaggcaat cggtcgttgg
    29041 ggaaatttca tgaaccacga agcatatgga ccagacacga cgcgaagctt cttagaaagt
    29101 ctgcatttac cgcaattcat cattgataat atgtatatcg aagggatcta tcgtcaacca
    29161 acatttcttt atgaatcact ttggagtttg attggattta ttttgctttt gtttttacga
    29221 aagaaaccac accttttgaa aaaaggcgaa gtccttttat gttatctaat gtggtatagt
    29281 ttcgggcgtt tctttattga aggattacgt acagacagtc tttacttttt aggtgtgatc
    29341 cgtatttctc aattattatc tgctgtgtta tttgtcggta cattgattct atttatttgg
    29401 cgcagaaaaa aaggaaattt accggattat gaccgctcat taggaaaaaa tcaacaaatt
    29461 atctgatttt tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa tgaattatag
    29521 agtagcggtt ttaggaccag gctcttgggg tactgctttg gcacaagtcc tcgccgaaaa
    29581 cggacatgac gttcggattt ggggacatcg tgcttcacaa gtagaagaaa tcaacaccca
    29641 acacacgaat caacgttatt taccagatca ttatctaccc gattccatcc aagcattcac
    29701 caatatggaa gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc caacgaaagc
    29761 aatacgttct gttgcaaaag aactagtgcc taaattgaat acaaagccag tgatcatcca
    29821 tgcaagtaaa ggattagaac agggaacaca taaaaggatc tctgacatct tgttagagga
    29881 aatcccagca gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc atgcagaaga
    29941 agtagcaaaa catgacatca ctacgatcac agctgcaagt acagatgcaa aagcagcaaa
    30001 atatgtacaa gagctgttca tgaataatta ttttcggatc tataccaatc ctgatgtgat
    30061 cggtgtagag atgggggcag ctttgaaaaa tattattgct attggtgctg gcgctattca
    30121 cggcttaggg tttggcgata atgcaaaggc agcaatcatg acgcgcggat tagctgagat
    30181 cagccgttta ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat taagcggaat
    30241 aggcgatcta gtcgttactt gcacaagtgt tcattctagg aactggcgcg caggcaactt
    30301 gttaggacaa gggcagcccc tagaagaggt gctagccaat atgggaatgg tcgttgaagg
    30361 tgtgaatact acccaagcag cgatggaact agcccaaatg atggaaatcg aaatgcccat
    30421 cactcaaaca atctatgaag tgctgtatga acaaaaagaa atcaaaacag ccgcaaaaga
    30481 aatcatgtta cgtgatggaa aaaaagaaaa tgaattctca ttaacaattt tttgaaggag
    30541 cgaatgtata tgaaagttaa aaaagcagtt attccagcag caggattagg aacaagattt
    30601 ttaccagcaa caaaagctat ggcaaaggaa atgttgccaa ttgttgataa accaacaatc
    30661 cagttcatcg ttgaagaagc gttgaaatca ggtatcgaag acatcttgat cgttacagga
    30721 aaagctaaac gaccaatcga agatcacttt gattcaaata ttgaattaga aacaaaccta
    30781 agtgaaaaag gaaagaccga attattgaaa ttagtagaag aaacgacaga tgtcaacttg
    30841 catttcatcc gtcaatctca tccaaagggt ttaggacatg ccgttcttca agcaagagcc
    30901 tttattggaa atgaaccatt tgtcgttatg ctaggcgatg atataatgga agacgaagta
    30961 ccattgacac agcaattaat cgatgattac gaacgtactc atgcttctac gatcgccgtt
    31021 atgcaagttc ctcatgatga aacttcaaaa tacggaatta tcaatccagg agaagtattg
    31081 gaagatgggt tatacaatgt caaaaacttt gttgaaaaac cagatccaag caaagcacca
    31141 agtgatttag ctattattgg acgttactta ttgactccag aaatttttga tgtattgtcc
    31201 gagcaacaac caggagccgg aaatgaaatc caactaactg acgcaatcga tacattgaat
    31261 aaaacacaac gcgtatttgc ccgtaaattc acaggtaaac gttacgatgt cggtgacaaa
    31321 tttggtttta tgaaaacaag tattgaatat ggtctaacac atccagaagt aggtgcacct
    31381 ttacgtgagt acatcctgtc tttaggtaaa acattagaag cagaaggaag caagatcgat
    31441 cataatccaa aaggaataac agaaatcaaa gaagaaaaga aaaaataacc cttttttcta
    31501 acgagcttca gctgctaatt gaataaaaag tagttgaagc tcgtttttat ttgtttaaaa
    31561 aacacgaaaa tgctatcctt atagaaaaga gagacaagaa gggagtttga tcatgtcagg
    31621 aggagaaatt gcaggattga tcgcagcaat cgcctttgcg atacttgtcg tttttatcgt
    31681 tcgtgtgttg ttgcaagttt ctaaaacttt agaaaaagta gatcagactg tagcagaagc
    31741 aaatacgacg atcgaaatcg tcacaaaaga cgtagatctt ttgtctagac aagtagaagg
    31801 cttactggtc aaaagtaatg aactgctctt ggatatcaat ggaaaagtcg caacgatcga
    31861 tccattgttc acagcagttg ctgatttaag tgaaagtgta tcagaactta atcattctag
    31921 taaaaacatc gcgacgaaag tcggcggaat cggtaaaacg acagctaaag cgacggttgc
    31981 cggaaaagtc ggtgggacag cgatgaaatt ttttaaaaac aaaaaacata ctgaaacgac
    32041 aggaggaaaa taaaaatggc taaaaaaggt ggatttttat taggtgcagt agtcggaggc
    32101 acagccgcag caatcgccgc gttattgtta gcaccaaaat ctggtaaaga acttcgggat
    32161 gaattagctg cccaagcgga tgatttgatg gatatggctt cagattacac agatatggcg
    32221 aaagaaaaat cgactgagtt aacaggtatt gcaaaagaaa aagcgcaaga tctttctcaa
    32281 caagcaggtg atctagcagg tagtgtcaaa caaaaagtaa gtgaatcagc agatgaagca
    32341 ggaagtgaat ctgacgatat cttgttaaat cttaaagaac aatcagctga cttatctaat
    32401 cgtttcaaaa aaacggcaga agatatgaaa aaacctgcag atgaattagg aagtattgct
    32461 aaagatactt cagaagatat catgattgat gtaaaagacg ctgctggtaa agcaggagat
    32521 actgtcaatg aaggaatgtc agaagcaaaa ccagttgcag atgactcaaa agaaatgatg
    32581 aaaatggcaa aagaagatgc gaaagaagct aaaaaagaaa taaaagatac aatgacagat
    32641 tcatctacaa atcactaaaa tcacaaattt atagaataaa aaggtgggag cggactagct
    32701 ctcacctttt ttactcttat ttttctaaaa caagtaattc ttttggtgta gtcgtgaatg
    32761 gcacacagcc atcgcttgtc acatatacac agtcttcgat acgtacccct actttttcag
    32821 gaatatagat tcctggttca agagagaagc acatcccttc ttcgattact aagtcatttc
    32881 cttctacaag ggaagggaat tcgtggacgg ttgttccgat cccgtgacct aaacggtgat
    32941 tgaaatattc gccgtagcca tagctgttga tcacgtttcg tgcgatttga tcaagttcac
    33001 ctgctgtcac acctggacgg acggcttctt gtgcggcaag ctgtgcttca agagtgatgt
    33061 tgtagatttt ttcttggaag tcggttggtt tttgataagc aattgtacga gtagcatcac
    33121 tgcaatagcc attccaaaca acgccaagat cgaataacac aagttcattc ggtgaaatct
    33181 tcgtatttcc tggtgtacca tgagggcttg cagcatttgc tcctgcaagg actaatgtgt
    33241 cgaaagacat ttgtgaaaca ccttgttttt tcaattggta ttcaatttct gcaatgatcg
    33301 cttgttcagt cacgccttct ttcactgctt taaagccaat ctcaaatgca acatcggccc
    33361 atttaccagc ttcgatcagt ttgtcttttt cctctagagt tttgatcagt tgtagacgtt
    33421 ggatgatgga agttagatca gtgtaagaat ccgttcccgt caagatatca gagatagcat
    33481 cgaagcgctc gacagtcaag gcgttctttt caatccctac attgccaggc tgaccatagc
    33541 gagcacgaag ttcttttccg atgatctccc aagggttttc gctgtctaaa tagccacgga
    33601 catcatacgc ccaactactg ttttgagcat cttcaacttc caaagcagga gtgaaaagga
    33661 aaggatcttg ttcaagaggg ataaaaagag ctagtacacg ctcatgagga tcactttcgt
    33721 atccggaaaa gtaagcgatg tggccagggt cgctgatata agccaattcg acttggtgtt
    33781 gtgccatcca ttgacgtagt tcattgattt ttacgttatt catttggcaa cctactttct
    33841 aaattaattt tgtattcaat aattgtacca ctgatttatt aaaagaccaa cgttctaaaa
    33901 actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa cggttgcaat tcttgagatt
    33961 atttgatatt ctattttaga aatgtaagac gtattttcag aaaacatata aaatatgaat
    34021 aggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag agaggcaaat
    34081 gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgttaa accagcaaca
    34141 agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaacgc agttgcgcgt
    34201 ggcttggcaa gtaagaaaac aacaacagtt ggtgtaatca tccctgatgt cagcaatatg
    34261 ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa atacaatatt
    34321 atcttagcaa attcagatgg caacgaccaa aaagaagtca atgtattgaa taacttactg
    34381 gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga aatccgtgga
    34441 gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc agatgaacaa
    34501 gtaggtagcg taaacattga ttatgtatcc gcaacaaaag atgcagtaaa caaattggca
    34561 aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc catcaatgga
    34621 caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc atatagcgaa
    34681 ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagt cgatcggatc
    34741 cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat cggtattttg
    34801 gatggtttat tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat cacaagtaac
    34861 aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca accattatac
    34921 gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga agaagtcgaa
    34981 gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa ataataaaag
    35041 gacaaccatt cagaggttgt gtgcctgccg tttaactctg cgttacaagg agcaatttca
    35101 aaaaacagca tctcatgttt gatgaatatt gtgactcgtg attcggagtt ggcaggagaa
    35161 caccgtttat ttgggaaaag gggttatgac aaaattttgt tataatcctt tttttgtgtc
    35221 ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa tccgtgtata
    35281 gatgctgcat tttgttcacc accatcaaaa aaataaggga ctgtgacaat actattgtca
    35341 caatccctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg attctttagt
    35401 tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat agttttcatc
    35461 ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg cccatagcga
    35521 ttcaactgtt tttcctggtg tttggatgga ttttttattg acagtaactt ttccaggtgt
    35581 ttgaccagta aatccagata cttttttctt catcacatca gatgataatt cgaatttctc
    35641 atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat tggcaagata
    35701 attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt gccctgccca
    35761 gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt attcattggt
    35821 agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc ttgagatggc
    35881 atctttgaat ggtgtagtga tttttgcatt gatcacacta cgcatcatgt cattcatgat
    35941 cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat aaattacatt
    36001 tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc cgccgtttgc
    36061 taaagcctga aatccgttgg tctgttgaag cgtcgtgaca tctactgtac ctaaaggagc
    36121 agattcaacg ccccaattgt catttgccgg gtagttcatc tttgccaagt agtgctcgta
    36181 agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat aagccgggat
    36241 attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc cttcgttagt
    36301 agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg cataatctga
    36361 tacacgggat tctgatccga taagccccat gtcgattgca ggtccataaa caagaactgg
    36421 tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt gattctgact
    36481 gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca tcaatacatt
    36541 tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat tttcaacgcc
    36601 attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat atcctttatt
    36661 ttggatcgtt tgctgtgcct tttgataata agcgtcactg accgatgaat cagacatatc
    36721 agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat tcatcacagt
    36781 ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa agtcttttgt
    36841 aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt cacgatacat
    36901 actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt ttagcgcgcc
    36961 tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga tataagcagc
    37021 ttgcggcagc gtcacatctt ttgcatctac accaaagatt ccttttgcag cttcctggac
    37081 tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca aatacgtagc
    37141 aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga tctcgtttgc
    37201 tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt gctgtgtcaa
    37261 agttgatcca ccagaagagc caataccagt agcttcagat actaatgctc gtaaaacagc
    37321 tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga taattgcagt
    37381 tttcaataaa ggtgaaatct tatcagatga aatagtcgtg cgcatcagat ctgtctggat
    37441 ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa tgtttcctag
    37501 ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat aggcaaaata
    37561 gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag taataaaaac
    37621 agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt ctttttttct
    37681 tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa ctaatggttt
    37741 tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt ttttggcagg
    37801 aatttctttt ttaaaagagt taaaacgtga gaaaatgatc tttattataa aagagttctt
    37861 aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga aaaatcaatc
    37921 attttctttg tatatcatct taccaatata taggaattgt tagtaacaat tgcctttcaa
    37981 ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag aaaatgctgt
    38041 taggagcagc agcagaagca gccagttatg gatcttcgac ttttatgatc tatacaggcg
    38101 cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc ggaaaagcgt
    38161 acatggcaga aaacggtcta acaaatatcg tcgttcatgc cccttatatc attaatttgg
    38221 ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga gaagagattg
    38281 ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct catgtagggg
    38341 caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt ttgacaaaag
    38401 aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa atcggtcgaa
    38461 catttgagga attagcgcaa atcatcgatg gagtaaaatt aaatgaaaaa ttatccgtta
    38521 cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac ttttcaggtg
    38581 taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc catatcaatg
    38641 attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt tttggaacaa
    38701 tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat atttcgaaaa
    38761 tcttggaaac tccctatgtc ggcaaagata aaaagaaagc ttacgcacca tatggaaaag
    38821 aaatcgccat gttcaaagca caaatattcg atccagaagt gtttcctgag ttattagcag
    38881 aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca ggcacattgc
    38941 cttgtccttt tttgtttaag aaacaaagga gtactttttg cagtcttttt catcatgcat
    39001 cttctccccc ctctttttaa atttagacaa acaaggtata ctgttagaaa agtaaaaaaa
    39061 cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga tgctgaacac
    39121 tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc ttggcatagc
    39181 cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg gaacagacat
    39241 gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt tggacggtaa
    39301 atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca gcttattttt
    39361 agtacttgca cataaactgc atctttctta tgctgttttg ttcggcatgt tcagtatttt
    39421 tgccagtata ttggttgcac tcgcgtttaa accgatcgtg aagaacaagt atcttctttt
    39481 aagtatctat ttttattttt tgttttctcc gattagtttt acacatgaat attccactcg
    39541 aacatatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt gtttactagc
    39601 tatttattac cgcaaatacg gttctagcag aaaattgctg ccatggtttt tagggctttc
    39661 tgtcgctttt cctttctttt ggttcattcg cgaagattct atctggcttc ttccttttac
    39721 tgtagtagct ttgggtatca gtttcgtcca gatcttgcta gggcaagact tttctttcaa
    39781 agaagtaaag aagttaaaaa aacgactctt tcttctctcg aaacgggaat ggctgaaact
    39841 ttcattgttt ttacttccta tagttggctt ttccatcgtc tatcagtgga tcgaatataa
    39901 aaatgagaca acatacggta ttgcaaccat caatgaccgt tccagtggag aatttggacg
    39961 tttgatgaaa caactgatcc gaatagatga tgaaacgaac ttgaataaag aaaacagtga
    40021 gatatgggtc tcacatgctg cactaaataa agcattagcc gcatctccta cattccatac
    40081 tgtttctacc cgtattgacg aattatatac gactcacgcg tggactcaag gtggaaaagt
    40141 aaaagaatta cccggagata tcatcttttg ggcattgcga gatgtggtaa gcgaatctgg
    40201 ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca accaagaatt
    40261 agaagaagcc tatcacaagg gaaccatcaa aaagaaaaaa gagttctatc tgatgggatc
    40321 aggagatgga aagacgatga gtgatctgcc aatcgtattc gatttccttc aatcaggata
    40381 cgcttatgca tggcgatata aagatttcca tcaaggagga aattatagtt tcggaacgca
    40441 agaacaagtc aaacaggctc aagaattatt aaaccttccg ctaatggaga attggatcga
    40501 tagtaatgaa ccgagaacac ctaatttcca attaacaaaa tcagcaaaag tcgcgaatgt
    40561 catcataaaa atttaccaag ttctggctat tcccgtgctg attgcttcaa tagcagggat
    40621 attacttatg atactaggaa ccgtatttgc aaaagctgat cgtgcctatt atagttcctc
    40681 tttgattgtg gtcaccggat taatactgac acaattggtt tttcttttcg gtgtctcgtg
    40741 gttttgttct tatgcgccag aaaaaaagga ttatttttta agtgtctaca ctggcgcagg
    40801 cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta aattaaatct
    40861 gaaaaaggct ttatcttcta aataaaaaga cccttggtag ctagattttt aacctaacta
    40921 tcaagggtct ttgtgtggaa aaatcagttt ctattcgatt tcttctaccg tttcctgcaa
    40981 acgctgttca tattttttgt ttcctttata taaatcacca atcgtccatg ccaacaggag
    41041 aaggacaaga acaatgatta cataagcgat gtgatcagcc aatgctagtt gagcagaagt
    41101 ggcttgttca ccaaagaaac tttcgatctg tcctggtaat cctcgtaagt tcaagtaagt
    41161 tagactaatc acagagaacc agccaagaag ttttacgatg aatgaatttt taaatcggct
    41221 acccatctcc gcttggctgt tggtcatcat cagtaaaggg atcatcgaaa acggcaacgc
    41281 aaaagccaag aacacctggg attcattcat caagttattc aaagcggtat gttcttcgat
    41341 cgttcctttt gtacttgtaa acaaaacaca aatcaataca ggaatcacag aaagcaatcg
    41401 tgtgaccagt cttcttagcc aaatcgggat acgcatatgg atgaagcctt ccatgatgac
    41461 ttgaccggtc aacgtaccag taatagtaga gttctggcca gaagcaagaa gtgctacagc
    41521 aaataaagtg gataagatac cagattttgc tacagcaatc aagataccat tgctcatagc
    41581 tgatggatca gataaggctt catacagacc aaaaaaggaa ggatcttgga cagaaccggt
    41641 tttgaagaca gcgacaccca tgatcaatag cagtgcattg actagaaaag ccatcgacaa
    41701 ttggatatta gagtcccaag tagaaaaacg tacagcttgt gctacatctt cttcgtcttg
    41761 gtgatcaatc ttccgagttt gagaaactgc tgaatgaaga tataagttat gcggcatgac
    41821 tgttgcacca ataatcccta aagctccagt cagcggagtt tcgccgccga tgaccggtga
    41881 gtctgagaat gtatgactgt tagggataaa gccagcaaat aaactgtgcc aatctgggtt
    41941 agacaagcca acttgataga caaaaacgaa taaaatgacg aaaattaagc agacaaccag
    42001 tgcttcgatc ttacgaaacc cgacttttgt cagtagtaag agaacgaaaa catctaatac
    42061 agtaataaag acggcaatca ccaatggaat atcaaaaagt aagtagaggg cgattgctgc
    42121 accaatgact tctgcgatgt ctgttgccat aatggctaat tcagtcaaga tccataaaat
    42181 gacacccaat gtcttacttg tgcgtgctcg gattgcttgg gccaaatcca tttgggtcac
    42241 gattcctaat tttgctgcca tatactgtaa aagcatagca atcagactag acatcaaaat
    42301 aactgacatc aataaatatt ggaaattttg tccaccagtg atcgaagtgg accagttacc
    42361 tggatccata taaccaactg ctactaatgc acctggtcca gagtaagcaa gaagtgtttt
    42421 aaaaaaactt ttcccctttg gtacatcaac tgttccattt acttcagcta aagataaacc
    42481 atttgtatgt tcaataagtc gatgcttatg aaattgtttg ttggattgat tattcaagaa
    42541 aaaaccacct ttcctattta tctacgatta cattctacac ctcaaaaaga aaaaattaaa
    42601 gaaaaatatt cggtatgcct aatttatttt cgtggcgcgt cttgttagat gacgaaagat
    42661 attttgttct cctctaaaaa aaccactatt ttttgcatga aactaaagaa tacgcctttc
    42721 ttttttcaat aaactttagt aaaataagtc attgagagca attcggaaat agaaaggaga
    42781 gatttatatg ttatcaacag gtattgtcgt acttattgct attattgctt tgctaatcgg
    42841 cgcagtaggt ggctttttcc tagcgcgtaa atatatgaaa gactatttag aaaaaaatcc
    42901 tcccgttaat gaggacatgt tgcgatcaat gatgatgtca atgggacaaa aaccatctga
    42961 gaagaaaatt cgccagatga tgcagcaaat gaagaaccaa gggaaaaaat aggttctgac
    43021 ccaacgactt tttacgggct gattttttca gtccgttttt tattttgtaa tttgttagac
    43081 tattcagatt attcagcaaa aagacaagca cagacaatcc taggctcttg ataaagacaa
    43141 aggaggagta aaatgtcgat ttttagaaag ttaggctggt tttttcgtca ggaaaagaaa
    43201 cattatttaa ttggggtttt ctcgctattc gcagtggcct tggtacagtt agttcctcca
    43261 aaagtgattg ggatcatcat tgatgagatt gccgataaaa atttatcagt aaagatcatt
    43321 ttcgggtgga tcttcctatt gattatcgct gctttcttac aatatctttt tcgttatatc
    43381 tggcggacga atatttgggg aagtgcagct cggttggaga aagaacttcg gcagcagtta
    43441 ttcaaacatt ttacaaaaat ggacaatctc ttttaccaaa aatatcggac tggggatttg
    43501 atggctcatg caacgaatga tttgaatgcg atccagaatg tagcaggagc tggtatcttg
    43561 acctttgcag attcagtgat tacgggcgga acaacgatca ttgtgatgat tttgtttatt
    43621 gattggcgct taacactgat tgcgctgatt cctctgcctt tgctggccgt cacttcacga
    43681 gtgctggggt cgaaactcca tgatgctttt cgtgattcgc aagaagcttt ttctagtatc
    43741 aatgataaga cacaagaaag tatcactggg atcaaagtca ttaaaacatt tggacaagaa
    43801 aaagaagatc tagcggattt tgaagaaaag attgaagatg caattattaa aaacaaacga
    43861 gtcaattttc tagactcctt atttgatcca ttcattacct tgattatcgg tctgtcttat
    43921 gttgcaacaa ttattttagg tggaaagtat gtcttggaag gcacgatctc gatcgggcag
    43981 atggtctcat ttattagtta tatcggtatg cttgtttggc cgatgtttgc gattggtcga
    44041 ttgttcaacg tattggaaag aggaaatgct tcttatgatc gcgtcaatga acttttgcat
    44101 gaaaaaacgc atatcgtgga acaactgggt gcagtgcaga ccccggtaaa aggcgagctg
    44161 aatgtagcta tttcctcttt taattatccg aaagaagaat caccagcatt agaacaaatc
    44221 aagtttgcaa tccaagaagg agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa
    44281 acaacgatca tcaaattact gatgcgtgaa tacgatgatt atcaagggaa aatccaattt
    44341 ggcggacgtt cgatcaaaga atattcattg aatgctttat taggatcaat cggctatgtg
    44401 cctcaggatc acttcttgtt ttcgatgacc attcgagata atatccgttt ttctaatcca
    44461 aaactttcag aagaagaagt agagcatgcg gcttctttag catttatcaa caatgaaatc
    44521 aaggaattac caaaaggtta tgatacctta gttggagaac gtggagtctc tttatctggc
    44581 ggacagaaac aaaggatttc gattgctcga gcattgatca aaaatcctga attattaata
    44641 ttagatgatg cactttctgc agtagatgcc aaaacagaag aagctatctt gcataattta
    44701 aaagaaataa gaaaagagaa aacaactatc attgcagctc accgtttaag tagcgtgatg
    44761 catgcaaaag aaatcattgt tgtcgatcaa gggaaaatca ttgaacgagg aacccatcat
    44821 gagctgcttg cactaggcgg ctggtatgaa caaatgtggg agaaacagca attagaagcg
    44881 aagatagaag gaggcgaggc ctgatggatg aaaattatca atcggaatgg acaaaatcga
    44941 ttccacttaa agaacagaaa aagatcatca aacgactgat gaagttcgcc aaacctttta
    45001 gaacaacgtt catcgttgct atcttatttg cgtttgctct gtctgtgatc aatgtcctat
    45061 tgccaaggat catccaaact tttatggatg atcatctggc taaacagtcg gctacgacac
    45121 aagttatttt gttttttgct ggtatgtact tatttggagt cctagtaaaa agtatcattt
    45181 ggtttttcca atggtatttg tattccatgg cttcattgaa gacctatcag tatattaggg
    45241 taaaattatt tgaaaagctg catacactag gtatgcgtta ttttgatcag acaccggcag
    45301 gatcaaccgt ttctcgagtg acgaatgaca cagaaacgct atttgaattt tggtacgtct
    45361 ttttgatggt attgactggg atctttgcag tcgtttcttc attcattgcc atgtttcaaa
    45421 tcaatggaga aattgccttg tggactttgg tgttcttgcc gattttagga attgtcatct
    45481 ggtattacca gaaatttagt tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc
    45541 tgaatacaaa attgaatgaa tcgatttccg gcatgcgtat tatccagcag tttcgccaag
    45601 aagctcgttt agctaaagaa tttgaacaag tgaacaatga gtatttagac acgcgctatg
    45661 cgatgatcaa aacgaattcg attctgttaa gtccgatcat caattttcta tatgcattgg
    45721 cgattgcgtt ggcgttgact ttatttggga tcgatgcgtt gcattctccc gtagaagttg
    45781 gactgatcta tgcatttacg acttatgtcc agggattttt taatccaatg tctcaaatga
    45841 tggacttttt gagcatcttc acagatggga tggttgctgg tagtcgtatc ttgaagatta
    45901 tggatacgga agaatatgca ccacaacaaa accctggagc agatcaaatg attacttcag
    45961 gtaagattga gtttcgcaat gtaagctttt cttatgatgg agagcacaat gtattaaaca
    46021 atatctcttt tgttgctaat ccgggagaaa ccgttgcatt agtgggacac accggaagtg
    46081 ggaaaagctc gatcatcaat gtgttgatgc ggttttacga attttatgaa ggtcagatat
    46141 tgattgatga tcatgacatt cgagaatatc caatggaaga gttacgtaag aaaatggggc
    46201 tggtcttgca agatgctttc ttgttttatg gggatatcgc aggaaatatt cggatgatga
    46261 atccagctat cacagacgaa cagatacgcc atgcggctga gtttgttcaa gcaaacaaat
    46321 tcatcgatga attaccaaaa ggatatcact caaaagtgat cgaacatggt gccagctatt
    46381 ctagtggaca acgacagctg atcacttttg cgcgaacaat cgtgactgat cctaaaattc
    46441 tagtattgga tgaagcaaca gcaaatatcg atactgaaac agaaggattg atccaagaag
    46501 gattggcgaa aatgcgacaa ggacggacaa cgattgcgat tgcccatcgg ctgtcaacga
    46561 ttcgagatgc agaattgatt ttagttttag acaaaggtca tatagtagaa cgcgggaatc
    46621 atgaaacact tctagcgcaa aatggtttgt atgcagatat gtacaaactg caaagtagtg
    46681 gtatataaaa gatgttgtga cagtagcatt ttgacctaag cactagagtg aaaataaaac
    46741 atagcttccc atatttttca tctgtgagag ttagtgtcgt agttaccaga tttaaaaaag
    46801 acgacgtcag ctagtcagaa gtgcctaata gcccctaaga aagcgttgac aaaatcgttt
    46861 gctttcgata atataaagaa aacgatacaa cttggatagt gattgcctga tgcaagctaa
    46921 acctgatgag tctcccatgg tttcatggac ggctttttgg gtttttcttt ttgttttgga
    46981 tacagcccta tatatcatgg aaaggagcaa aacatgctga ttgagaaagt actgaataac
    47041 aacgtcgtaa taacaaggga tgaaagagat cgggaaatga ttgtgatggg aaaaggattg
    47101 gcattcaaaa agaaggtggg tgacccgatt cttgactcat gcatcgacaa aaaatttcag
    47161 ttgacaaatg acggactttc acaacagttc caagaattgg cacaagcgat tcctcttgag
    47221 tacttagaaa tttcttgtga aatcattgaa tatacaaaag aggctctgca agtccattta
    47281 aatgattctg tgtatatttc attgacggat cacttgtata cagctatcga acgtcaaaaa
    47341 atggggatct ttgtgccaaa tgtattgctg tatgatatca aacaactgtt tcctaaagag
    47401 tatgccattg ggaaaaaaac agtgaagaaa atctcggata aataccacat caagttatca
    47461 gaagacgaag ccggatttat cgcattgcat ttagtcaatg cacaaggcat tcaaagccag
    47521 caagacaata cctacctgtt taccacaaca atacaggata ttttaaatat cgtccgctat
    47581 tactataaga ttacatttga tgaagattca gtccactttt ttcgattcgt cacacacctg
    47641 cgtttttttc tgacacgtgt cctagatgga gcgaaatcag aagatggaga agtttctgag
    47701 gaattgttcc aagttattca aaaaaaatat aaggatacgt ttggttgcgt tgagaaaata
    47761 gcagactttt taaccggggc tttcgactac cagatgtcca cggatgaaaa aatgtattta
    47821 acgatccata ctgcgcgttt agtgcatgag aatcgttgta aataaataaa aaaggaggac
    47881 aaattaacaa gaacgtatga aataagggta gtgacattca gttggctaaa ccttccacta
    47941 attaattgga gggaatagaa atgggaaaat accaggaatt agcagaaaac atcgtaaaga
    48001 atatcggagg aaaagaaaat atccgtagct tgacacactg tgtgacgcga cttcgtttca
    48061 aactaaagga ggaaaataaa gcaaaggatg atgttttgaa aaagatggaa gggatagtca
    48121 ccatcatgaa aagcggcggg caatatcaag ttgtaatcgg gaatcatgtg ccagatgtct
    48181 atgcagaagt actgacagta gccgggattt ccgatgaagc agaagaagag gtttcttcaa
    48241 gtagtaatct gttcaatcga ttaatcgaca tattaagtgg ctgtttccag ccttttttag
    48301 gtgctttggc tgcagcggga atggtcaaag gattaaatgc tttgttcttg tttctcaaat
    48361 tttatactga aggatcgggt acgtacatca tgctgaatgg gataggggat gcaatttttt
    48421 atttcatgcc agtgatctta gggtacacag cagcgaaaaa atttcatctg aatccgatgg
    48481 tggggctcgt gattggtgca gcactttgtt atccgactgt acaaggagca gcactacaaa
    48541 gtggtttccc tgatggcagt cctgcgcctt atagcttgct gggtcttccg gcttatgaaa
    48601 catttttggg tatcccatgg gtgggcagca gttatactag tagcgtcgta cctgttatct
    48661 tcattatcgc atttgccgga caagtccaaa aaattgccaa gaaatggatt cctgcagtcg
    48721 tccagacctt tttggtttct ttttctgtgt tattaatcgc acttcctgct ggctttttga
    48781 ttatcggtcc gattgtcagt atactgacag atctattaag cacgggattc caagcattga
    48841 tggacttttc tccagctgtg tatggtgcag ttctagggtt tctttggcaa gtactggtga
    48901 ttttcggact tcattggagt attatccctc ttgcgattat gcagctagcg caaaatgggt
    48961 ggagtcagat gttagtaggc gtttatggcg cgagttttgc acagacagca gtggtattgg
    49021 cgatgtattt caagttcaag gatcaaaaaa tgaaggcgct ttgcatgcca gctgtgattt
    49081 caggaatctt cggcgtaaca gaaccggcta tttatggaat caccttaccg aagaagaagc
    49141 catttattta ctcgatgatt ggtggtgctg tttcaggaat ctatttgatg gcatctggaa
    49201 caatcagcta cataatgggc ggattaggga tttttggggt ggtaaactac atcacggaaa
    49261 cagacgccag tggtatgttt caagcgttta tcggcattgc tattgcttcg gtaatcgggt
    49321 tttcgctgac ttttttcttt tggaaggatg aagcaggtga aacagatgct atacagaaaa
    49381 aatcaggtaa agaaattgta aaaacaccga tacaaggaca aatcgctcca ctaaatgcag
    49441 cgaaggatgc agcttttgca caaggaacac taggtcgagg aatcttaatc taccctgaaa
    49501 aaggagaagt acgtgcccct tttgacggaa cagttatgac cctgttcccg acgaaacatg
    49561 caatcggaat ggtttctgaa acagggctgg aattgctgat tcatgtcgga ttggatactg
    49621 tgcagttgga agggaaatat tttgaaagct tagtgcagca aggagaaaga gtgagacaag
    49681 gcgatctgtt gctacgcttc gacaaaaaga aaatcgaaaa ggaaggatac agtttagaaa
    49741 cacctgtgat cgtcaccaat tacacggatt atttggatat tttggaaaac caaggggaga
    49801 cggtcgggcc agacgatgca cttatcaccg cactgacctg aacggttagg tgaagaatgg
    49861 actaggctta actattcttt ttattccact tctttatttt caaaattgaa aggctctttc
    49921 atgatggctg agctgatagg gcgtttatcg aatttttgac cgacagaaat tagatggact
    49981 aaattctgtt gataattttc tgcaaaaaca caactatata acacgtctag aagataacaa
    50041 attgatgtat tgatcgtaaa attaccaatt tttgaataca gtcgctcgcg tgtcgtaagt
    50101 cgcaaagcgg ctgtactcat tgcagatagc gttgaatcac cgatactggt caaagctaaa
    50161 attggaacat cattttgttt caatatttct gcggtacgaa ggatcacatt gttttcaccc
    50221 gtataagaaa tcagaatccc gcacatatcg ctcgtacaat tgtacgcttc atagaatgat
    50281 tcaccaaaag tggtgacaat tgtgacatcc attttgatgc gcttcatttt cagcatgaaa
    50341 tcttgagaaa tcaaagtatt cgagttactg gcgaagatca cgattttttt tgcctggagc
    50401 agtaaatatt tagctttttg cagttggtca tgatgaatga gagaaagtgt atcatgaatc
    50461 gtcatctgtt ctaaagaagc cattttattt gcgattgtca taataccgtc tgtggtggaa
    50521 aaaggaagat tcgcatctac gtcttgaaaa ttcccttcaa gataggctgt ttcagctaaa
    50581 aaagcattac gaaaactgac ccagccatca aaacccattt tttttgctac acggatgagt
    50641 gtagacgggt ggacataaca tcgctcagct atcgcttgga tggtcatttc gccaagtgct
    50701 ggagaatcgc ttaagagaaa ctggatcact tcattttctg aaggagaaaa ggagatttct
    50761 ttgatttttt cacgtaataa catgtagata ccttcttctt atttttttac gtaaattata
    50821 acttttttga aaatgttttg taaacggatt tatactatat aacttaaaat aggctttttt
    50881 tgacaagata tttacaaaaa tccactagaa aacagtaact tttgtaaatg ttactggaaa
    50941 gaaacggatt tctattgttt gttttgttta caaaaaagat aatgaaagtg taaaaaagta
    51001 aaggagtgag aacatggcaa aaggaatcaa aatcgctacg attggtggag gcagcagtta
    51061 cacccctgag ttgatggaag gatttatcaa aagatatgag gaattaccga tacgagaaat
    51121 ctggctcgta gatgtagaag aaggaaaaga aaagcttgag atcgttagtc agatgtctca
    51181 gcgtatgtgg gatgcttctc catatgaagt caagatctat gcaactctgg atcgggaaga
    51241 agcattgaaa gatgcggatt ttgttacgac tcagtttcgt gtcggtcaat tagatgcacg
    51301 aatcaaagac gagcggatcc ctagttatta tgggatgatt gggcaagaaa caaatggtgc
    51361 tggcgggata ttcaaagctt ttcgtaccgt tccagtcgtt ttggcaatcg ttgaggacat
    51421 gaagcgttta tgtccaaatg cttggctgat caatttcgcg aacccaagtg ggatggtgac
    51481 agaagcagtt atcagatatg gcaaatggga taaagtgatt ggattatgca atgtgccagt
    51541 gatggcgatg atgacagaac cagcaatgct tggcaagaaa aaagaagagc tgatctacaa
    51601 gtttgccggt ctaaatcatt tccattggca caaagtagca gataataaag gaaatgaccg
    51661 cacggcagaa ttgattgatc gtttatacga tgaagataat ggattaccga aaaatatctt
    51721 tgatatcccg tttttcaaag aacaattaaa acaaatgaac atgatccctt gtggctacca
    51781 ccgctattac tatcgtgaag aagaaatgct ggaacatgct ttagaagaat atcaaacgat
    51841 tggtacaaga gcgcagcaag tgaaagaaac agaagcagag ctatttgaat tatacaaaga
    51901 tccagaatta gaccataaac ctgaacaatt gcaaaaacga ggtggcgctt attattcaga
    51961 cgctgcctgt gaaacgattg cttcaatcta tggaaataaa gaaacacaga tcgttgtatc
    52021 tacaaagaat gatggagctg ttcctgacct gtcggcagat tgtgttgtag aagtcactgc
    52081 ctatgttggc gcacaaggag cgagaaatgt agcgtttggt tctcttcctg ctgctgaaag
    52141 aggctggttg caagtaatga agaatatgga attgctgacg atcgaagcag ctgtgactgg
    52201 tgattatggg acagctttac aggcgtttac gatcaatcca cttatacgat caggaaagac
    52261 agcaaaacgg atcatggatg agttgtttat tgcacaccag cagtatttac cgcaattcaa
    52321 ggaaacaatc agccgattaa aagaagaagg aatcgaagtt caagatgaac tggcaagaga
    52381 attatcaggt aatacacata taatggagga agtataatgg ggttcagaaa agatttttta
    52441 tggggcggtg ctacagcagc caatcaatgt gagggcgctt atgatgaagg tggacgcgga
    52501 ctagcaaacg tagatgttgt gccaatcgga aaagaccgtt ttcctgttat caccggaaag
    52561 aaaaaaatgt ttgaatttga tgaggatcat ttttatccag caaaaattgc aatcgatatg
    52621 tatcatcgtt ataaagaaga tattgctttg tttgcagaaa tggggttcaa gacttatcga
    52681 atgtctatcg cctggagtcg tatttttcca atgggggatg agaaagagcc gaatgaagct
    52741 ggcttgaaat tttatgagga tgtctttaaa gaatgccaaa agcacggaat tgagccactt
    52801 gtgacgatta cgcattttga ttgtccaatg catttgatca aagaatatgg tgcgtggcgt
    52861 tccagagaac tcgttggttt ttatgaaaat ctgtgtcgag tcatcttcaa tcgctataaa
    52921 ggacttgtca aatattggct gacattcaat gagatcaata tgatccttca tgcaccattt
    52981 atgggcgcag ggctgtattt tgaagaagga gaaaatgaag aacaaatcaa atatcaagcg
    53041 gcacatcatg agttggttgc aagtgccatc gcaacgaaga ttgctcatga agtagatccg
    53101 gagaacaaag taggctgtat gctggcagca ggagcatatt atccatacac ttgcaatcct
    53161 gaagatgttt gggcttctag aaaatcagat cgagaaaatt atttcttcat tgatgtccag
    53221 tcacgaggag aatatcccgc ttatgcattg aaagaaatgg agcgaaatgg cattcagatc
    53281 gaaatggaac caggggatgc cgcattgctg aaagaacata ctgttgattt tatctctttt
    53341 tcttattatt cttcacgagt ttcgactacc gacaacgaac tcttggaaca gactgcagga
    53401 aatatctttg cttctgtcaa aaatccatat ctgcaagcaa gtgaatgggg gtggcaaatt
    53461 gatccattag gcttgagaat aacgatgaat gatatttatg atcgttatca aaaaccatta
    53521 tttattgtag aaaacggctt aggtgcagtc gatacgccag atgaaaatgg ttatgtggaa
    53581 gatgattacc gaatcgatta cctggcagcg catatccagg cgatgaagga tgcagtagaa
    53641 ctagacggag tcgatctatt aggctatacg acttggggat gtatcgattt agtatcagct
    53701 ggtaccggtg agatgaagaa aaggtacgga ttcatttatg tcgatcgtga taaccaagga
    53761 aatggcacat tagcccgttc aaagaaaaaa tcattcaact ggtataaaaa agtaattgat
    53821 tcaaatggag aaaatctttc gaacaattga tcaatgattt ataaaagtct attgtttttc
    53881 agcggatttt tcgagtataa taaagctatt cgagaaacgc aagcagaaca atagactttt
    53941 tttcgcattg gctgtaaagt atgcttactt gattaagtaa aggagaatct acatatgtat
    54001 gatgtaatcg ttattggcgc aggtcctgct gggatgactg ctgctttgta tgcttcacgt
    54061 tcaaacttat ccgtattgat gatcgaacaa ggtgcccctg gcggccaaat gaacaatacg
    54121 gcagaaatcg aaaattatcc tggattcgat tcgatcatgg gccccgatct agcttataag
    54181 atgtatgaaa gtgtctcaag atttgggaca gaaaatgctt atggaatcgt tcaagatatc
    54241 aaggatcacg gcagccataa agaagtcatc tgtgaagata aaagttacga aggaaaaaca
    54301 gtcattatcg caacaggctg tatccaccgt aaattaggcg tacctggcga ggaagaattt
    54361 gctggacgag gcgtttctta ctgtgcagtc tgtgatggtg cgtttttccg taacaaacgt
    54421 ctagtagtca tcgggggtgg ggactcagca gtagaagaag ccatctattt gacacaattt
    54481 gcttctgaag tagtgatcgt ccaccgtaga gatgaattac gtgcacaaaa gatcatccaa
    54541 gaccgtgcat ttgcaaatga aaaaatttcc tttatgtggg atacagtcgt tgacgaaatc
    54601 gttggaaacg aaatggtagt gacaggtgtg aaagcacgta atgtcaaaac cggcaatgaa
    54661 tttgatttag cagcagacgg agtattcatc tatgttggac tggatccatt aactgcacct
    54721 tttagagaag caggtttgac gaacgaagct ggctggattc caacagacaa cgaaatgaaa
    54781 acgaatatgc caggcgtttt tgccatcgga gatgttcgtg aaaaagattt gcgacaaatc
    54841 actacagctg ttggtgacgg cggtattgct ggacaacaag tgtataaata cttggagtct
    54901 ttagaagaaa caacagcata agtttaaaaa gagtgaaaga acggtttcaa ttgttctttt
    54961 actctttttt ttcgcgtgaa atccactgaa tccgatttat gtgaatactt gcaatcatgc
    55021 ttgataaaag aatcctttct tgatttaagc gttttttggt cctcatactt tcatatctaa
    55081 taagtgaatg ctatacttat taagaaccat atttcagaaa gaggtgttat tttcatgtct
    55141 tgggaacaag tgtatgaaca atgggtacat gaagaaaatt tagaagaaaa cttaaaaaaa
    55201 caattaaatg atctgggtga agatcctgaa aaacttgaag atgcttttta tgcaccgttg
    55261 gaattcggta ctgcaggcat gcggggaatc ttaggacctg ggatcaatcg aatgaacatc
    55321 tatacggtaa gacaagcaac agaaggacta gcacgtttca tggatacaca aggtgaagaa
    55381 acaaaacgcc gaggtgtcgc tatcgcttat gactcacgcc atcagtcacc agaatttgca
    55441 atggaagcag ccaaaacact ggcacatcac aatatcccgt cttatgtatt cgaaagtctg
    55501 cgtccaacac cagagctttc ttttgcagtt cgtcatttga agacgtttgc gggaatcatg
    55561 gtaaccgcat ctcacaaccc agcggcttac aatggatata aagtctacgg agaagatggc
    55621 ggacaaatgc ctcctgcaga cgcagatgca ttgacgaaat atgtccgtga agtcagcaat
    55681 ccgctaaaag tcgaagtatt atctgacgaa gaagctaaac atagcgggtt gatcacgatc
    55741 atcggagaag aagtggacgc tgcttatcta gaagaaatca aagcagtgac gattgatcgt
    55801 gaattggttg aaacaatggg gaaagatttg aaacttgtgt acacgccact tcatggaaca
    55861 gggaaaatgt tgggcgaacg tgcgttgaaa caagctggat tcgaacaatt cgtactagtt
    55921 cccgaacaag ctgtggcaga tccagatttc actacggtca aatcgccaaa tccagaagaa
    55981 cattcagctt ttgaatatgc tatacgactt ggagaaaaag agggagctga cttgctgatt
    56041 gcaacagatc ctgatgcaga ccgtttaggc gcagcggttc gtttgccaga tggttcttat
    56101 gaagtattga ctgggaacca aatcggagca ttgtttgctc aatatatttt ggaagcacat
    56161 caatcaagag gcactttacc agaaaacgca gcggtattga aatcaatcgt ttccagcgaa
    56221 ttgcctacag caattgcgga acactacggt gctactatgt tcaatgtgtt gacagggttt
    56281 aaattcattg ctgaaaaaat ccaacaattt gaagaagacc attcacatac ttttatgttt
    56341 ggattcgaag aaagttatgg ttatttagtt aaaccttttg ttcgcgataa agacgcgatc
    56401 caagcattgc ttttattagc agaagtagca gcttactata aaaaacaagg caaaacgttg
    56461 tatgacggct tgcaaacgat ttttgaaacc tatggctatt tcgcagaaaa aacgatttct
    56521 gtgacaatga gcggacaaga aggttctgcg aaaatctctg ctttgatgga aaaattccgt
    56581 aaagaagcac cgacagaatt tgccggagca aaagttattc aaacagaaga ctttaaagaa
    56641 ttgacgagaa cagaccgtga aggaaacgta gaaaaactaa caacaccgcc atccgacgta
    56701 ttgaagtata cattggaaga aaatggctgg atcgctatcc gcccttcagg aactgagccg
    56761 aaaatcaagt tttatattgg tgtaaaagga agctctacag aagacgcagc cgaaaaagta
    56821 gcctcttatg aagcgattat caaaaagatg acagaagaat aaaaaacttg agactgatgg
    56881 aaaaagtggt aaactttaga agtcagtctt ttttcaagtt ggaggtgaat ggattgccag
    56941 acacgcaaaa atcaaaagta gagaaagtca gtcaattatt caaaatgttg agtgacccca
    57001 ctcgtcttaa aattctgcta tatctgaaag acggggaaca aaatgtgaca gcaatcaccc
    57061 aagcagtgga aatggagcag tctgctgtat cgcatcaact acgtttatta agagaaaatc
    57121 atgtcgtcaa gtctcatcgc gagggaaaag cgattttgta tagtcttgat gattctcatg
    57181 tactggatat tttgaaccag acactcaaac atgtggaaca tcaataacga taggtgcaaa
    57241 taga
//
LOCUS       DAZAFF010000005        53977 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000005 DAZAFF010000000
VERSION     DAZAFF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.005"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..540
                     /locus_tag="ACG9BB_000338"
     CDS             19..540
                     /locus_tag="ACG9BB_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2836316.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            637..858
                     /locus_tag="ACG9BB_000339"
     CDS             637..858
                     /locus_tag="ACG9BB_000339"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN2836317.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1015..1179)
                     /locus_tag="ACG9BB_000340"
     CDS             complement(1015..1179)
                     /locus_tag="ACG9BB_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFN2836318.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1282..1803
                     /locus_tag="ACG9BB_000341"
     CDS             1282..1803
                     /locus_tag="ACG9BB_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFN2836319.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1790..2230
                     /locus_tag="ACG9BB_000342"
                     /pseudo
     CDS             1790..2230
                     /locus_tag="ACG9BB_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(2270..2428)
                     /locus_tag="ACG9BB_000343"
     CDS             complement(2270..2428)
                     /locus_tag="ACG9BB_000343"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFN2836320.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2521..3282
                     /locus_tag="ACG9BB_000344"
     CDS             2521..3282
                     /locus_tag="ACG9BB_000344"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN2836321.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3469..4821
                     /locus_tag="ACG9BB_000345"
                     /pseudo
     CDS             3469..4821
                     /locus_tag="ACG9BB_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            4837..6057
                     /locus_tag="ACG9BB_000346"
     CDS             4837..6057
                     /locus_tag="ACG9BB_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN2836322.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6092..6769
                     /locus_tag="ACG9BB_000347"
     CDS             6092..6769
                     /locus_tag="ACG9BB_000347"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFN2836323.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6766..7962
                     /locus_tag="ACG9BB_000348"
     CDS             6766..7962
                     /locus_tag="ACG9BB_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN2836324.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7977..8300
                     /locus_tag="ACG9BB_000349"
     CDS             7977..8300
                     /locus_tag="ACG9BB_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN2836325.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8304..8639
                     /locus_tag="ACG9BB_000350"
     CDS             8304..8639
                     /locus_tag="ACG9BB_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN2836326.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8636..9031
                     /locus_tag="ACG9BB_000351"
     CDS             8636..9031
                     /locus_tag="ACG9BB_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFN2836327.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9028..9399
                     /locus_tag="ACG9BB_000352"
     CDS             9028..9399
                     /locus_tag="ACG9BB_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836328.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9415..9975
                     /locus_tag="ACG9BB_000353"
     CDS             9415..9975
                     /locus_tag="ACG9BB_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFN2836329.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10102..10413
                     /locus_tag="ACG9BB_000354"
     CDS             10102..10413
                     /locus_tag="ACG9BB_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836330.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10437..10628
                     /locus_tag="ACG9BB_000355"
     CDS             10437..10628
                     /locus_tag="ACG9BB_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836331.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10628..14473
                     /locus_tag="ACG9BB_000356"
     CDS             10628..14473
                     /locus_tag="ACG9BB_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFN2836332.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14474..15346
                     /locus_tag="ACG9BB_000357"
     CDS             14474..15346
                     /locus_tag="ACG9BB_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFN2836333.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15343..16392
                     /locus_tag="ACG9BB_000358"
     CDS             15343..16392
                     /locus_tag="ACG9BB_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836334.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16396..17220
                     /locus_tag="ACG9BB_000359"
     CDS             16396..17220
                     /locus_tag="ACG9BB_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836335.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17238..19370
                     /locus_tag="ACG9BB_000360"
     CDS             17238..19370
                     /locus_tag="ACG9BB_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFN2836336.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19387..19536
                     /locus_tag="ACG9BB_000361"
     CDS             19387..19536
                     /locus_tag="ACG9BB_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836337.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            19533..19979
                     /locus_tag="ACG9BB_000362"
     CDS             19533..19979
                     /locus_tag="ACG9BB_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836338.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19981..20118
                     /locus_tag="ACG9BB_000363"
     CDS             19981..20118
                     /locus_tag="ACG9BB_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFN2836339.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20155..20448
                     /locus_tag="ACG9BB_000364"
     CDS             20155..20448
                     /locus_tag="ACG9BB_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836340.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20471..20668
                     /locus_tag="ACG9BB_000365"
     CDS             20471..20668
                     /locus_tag="ACG9BB_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFN2836341.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            20679..21698
                     /locus_tag="ACG9BB_000366"
     CDS             20679..21698
                     /locus_tag="ACG9BB_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN2836342.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            21955..22602
                     /locus_tag="ACG9BB_000367"
     CDS             21955..22602
                     /locus_tag="ACG9BB_000367"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN2836343.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(22927..23073)
                     /locus_tag="ACG9BB_000368"
                     /pseudo
     CDS             complement(22927..23073)
                     /locus_tag="ACG9BB_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            23312..24004
                     /locus_tag="ACG9BB_000369"
     CDS             23312..24004
                     /locus_tag="ACG9BB_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN2836344.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAKIDEINQKIAEINQLNTDWNNRLQTEQNKFNDAQNT
                     VNSLNQQINQLSNEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTAN
                     RSQEIVTEHTGK"
     gene            complement(24286..24358)
                     /locus_tag="ACG9BB_000370"
     tRNA            complement(24286..24358)
                     /locus_tag="ACG9BB_000370"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24323..24325),aa:Lys,seq:ctt)
     gene            24739..25308
                     /locus_tag="ACG9BB_000371"
     CDS             24739..25308
                     /locus_tag="ACG9BB_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFN2836345.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            25295..26155
                     /locus_tag="ACG9BB_000372"
     CDS             25295..26155
                     /locus_tag="ACG9BB_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN2836346.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            26345..27049
                     /gene="yycF"
                     /locus_tag="ACG9BB_000373"
     CDS             26345..27049
                     /gene="yycF"
                     /locus_tag="ACG9BB_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFN2836347.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            27054..28889
                     /gene="walK"
                     /locus_tag="ACG9BB_000374"
     CDS             27054..28889
                     /gene="walK"
                     /locus_tag="ACG9BB_000374"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFN2836348.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            28886..30202
                     /gene="yycH"
                     /locus_tag="ACG9BB_000375"
     CDS             28886..30202
                     /gene="yycH"
                     /locus_tag="ACG9BB_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFN2836349.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            30203..31072
                     /locus_tag="ACG9BB_000376"
     CDS             30203..31072
                     /locus_tag="ACG9BB_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFN2836350.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            31127..31936
                     /locus_tag="ACG9BB_000377"
     CDS             31127..31936
                     /locus_tag="ACG9BB_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN2836351.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(32039..32668)
                     /locus_tag="ACG9BB_000378"
     CDS             complement(32039..32668)
                     /locus_tag="ACG9BB_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN2836352.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            32838..34130
                     /locus_tag="ACG9BB_000379"
     CDS             32838..34130
                     /locus_tag="ACG9BB_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN2836353.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(34206..34868)
                     /locus_tag="ACG9BB_000380"
     CDS             complement(34206..34868)
                     /locus_tag="ACG9BB_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN2836354.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            35093..35377
                     /gene="groES"
                     /locus_tag="ACG9BB_000381"
     CDS             35093..35377
                     /gene="groES"
                     /locus_tag="ACG9BB_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFN2836355.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            35428..37053
                     /gene="groL"
                     /locus_tag="ACG9BB_000382"
     CDS             35428..37053
                     /gene="groL"
                     /locus_tag="ACG9BB_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFN2836356.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            37466..38653
                     /locus_tag="ACG9BB_000383"
     CDS             37466..38653
                     /locus_tag="ACG9BB_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFN2836357.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(38913..40064)
                     /gene="fucO"
                     /locus_tag="ACG9BB_000384"
     CDS             complement(38913..40064)
                     /gene="fucO"
                     /locus_tag="ACG9BB_000384"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFN2836358.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(40146..40457)
                     /gene="rhaM"
                     /locus_tag="ACG9BB_000385"
     CDS             complement(40146..40457)
                     /gene="rhaM"
                     /locus_tag="ACG9BB_000385"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFN2836359.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(40459..41304)
                     /gene="rhaD"
                     /locus_tag="ACG9BB_000386"
     CDS             complement(40459..41304)
                     /gene="rhaD"
                     /locus_tag="ACG9BB_000386"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFN2836360.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(41318..42574)
                     /gene="rhaA"
                     /locus_tag="ACG9BB_000387"
     CDS             complement(41318..42574)
                     /gene="rhaA"
                     /locus_tag="ACG9BB_000387"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFN2836361.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(42571..44031)
                     /gene="rhaB"
                     /locus_tag="ACG9BB_000388"
     CDS             complement(42571..44031)
                     /gene="rhaB"
                     /locus_tag="ACG9BB_000388"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFN2836362.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            44184..45053
                     /locus_tag="ACG9BB_000389"
     CDS             44184..45053
                     /locus_tag="ACG9BB_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN2836363.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(45179..46078)
                     /locus_tag="ACG9BB_000390"
     CDS             complement(45179..46078)
                     /locus_tag="ACG9BB_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2836364.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(46243..47067)
                     /locus_tag="ACG9BB_000391"
     CDS             complement(46243..47067)
                     /locus_tag="ACG9BB_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN2836365.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(47078..48373)
                     /locus_tag="ACG9BB_000392"
     CDS             complement(47078..48373)
                     /locus_tag="ACG9BB_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFN2836366.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(48389..48868)
                     /locus_tag="ACG9BB_000393"
     CDS             complement(48389..48868)
                     /locus_tag="ACG9BB_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFN2836367.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(48874..49902)
                     /locus_tag="ACG9BB_000394"
     CDS             complement(48874..49902)
                     /locus_tag="ACG9BB_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFN2836368.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(49919..51025)
                     /locus_tag="ACG9BB_000395"
     CDS             complement(49919..51025)
                     /locus_tag="ACG9BB_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN2836369.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(51254..53851)
                     /gene="adhE"
                     /locus_tag="ACG9BB_000396"
                     /gene_synonym="adhC"
     CDS             complement(51254..53851)
                     /gene="adhE"
                     /locus_tag="ACG9BB_000396"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFN2836370.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT        17613 a         9991 c        11014 g        15359 t
ORIGIN      
        1 gattaaggag gaagagtaat gcatatagga gataagatac ataaattgag acggcttaat
       61 aacgaaacaa tgaacgactt agttaaacaa accggactgt caaaatcatg ggtatctgat
      121 attgagtctg gaaaaaagca acgcgctgac tttaataaga tatgtgcaat agccaaacat
      181 tataaagtta gcttagaata ctttcaggat gattatttgg atagtgatga acacgaggat
      241 attattcctt tgacagacga aacaagagtt aaatcagtga aatcgcttga acaaagccta
      301 aagtatctga ataacaaaca agaacctaac cttctagaga tcaaactaaa agatactgac
      361 tcagtaccag aggtttatta caaaggtcag aagttagagg gattgataga tgtaagctat
      421 cactaccata caacaactga tgtggtagct ggttcaacaa acgacatcaa tatggagtac
      481 ctaggtgaag taactaagta tccagatata aaaacaatcg gacacaagag agatgtgtaa
      541 atgaaagaag ctagacctag agacgagata gacaaactat acaagaccaa acgatggcga
      601 gacctaaggc aagtagtaat agctagggac ttcggcatgt gccaagagtg caagcgtcga
      661 gggcggaaca caaggggcac gatcatccat cacatagtcg aggcgaggga agacctgtca
      721 ctgttctggt ccgtagataa ccttgaatgt atctgtgtag cttgtcacaa cagagagcat
      781 ccagagagat caggcgggaa gaagaaacca aaacctaaat cacatatcgt taaaatgtat
      841 tcaactcctg aaagataagt ttgcagcgaa atgaaggtag cccccctact ctaaaagatt
      901 aaagagtaag gcttgagaag aacggtgctg tccttccttc gtaaaaagac cgcttttcaa
      961 gttttttgga gaaaaaggaa aaagccgacc aatttaagcc ggctttggac gaagctattt
     1021 cttagtccat ttgtttcctt tttgagaagt aggaggtaat cggtcgcctg gatcaatttt
     1081 tacttctcgt ccgccttgga catttccacc acgaggtccc acttctttat aggttccttt
     1141 tggtttattg tcttcgccgg gtttatagag ttctcccata ggaatccctc cctaaaaaaa
     1201 tttcggcaca gcactgccga taacttaatt ataaggattg tgataacgat ttcaatatat
     1261 cttttgaaag aaggtgatat tatgccgcaa ccagcgaaga gtgcaaaatt acaattatta
     1321 aacggaaacc caaataagaa gaataccgaa gaactccgca agcgagcggc cgcagaagac
     1381 aaattaaaaa tggctactga caaaatcaaa ccgccgttat ggctagattc gctaggaaag
     1441 gatacctttg agtttatcgc tgatgaattg ctgtctgtgg atttaatcag taatccggac
     1501 gtccatacaa tggctctcta ctccaattgg tattcgcaat acgtttcttt agaaaaacag
     1561 cttcgaaaac tacaacgaga gtacaagttg aactatgcgc ttgcgaaaaa ggaggcagag
     1621 gcgagaggtg agccgtttaa tgaacctaat gaattaattg gtaatccgct ctctcggcag
     1681 atggatacag cgtcgcggaa tctccgttct tttggcgctg atttaggact atcaccagca
     1741 gccagagcta agttagctat taagatggct gatgatggtg gtgatgacga tgacgacttc
     1801 taatattttg gatatgtcct acacagaacg tgtggactat tggcaaagct atcttgagga
     1861 gcaagcttct tggggtggct ttttaaaatg tccatatccg gaattgttaa ctacttggta
     1921 tgcggaacga ttaatcgatg gaagcatacc agccagcaaa gaaaatattc aagctgctaa
     1981 acggcatatg cgtgatttgc agcgccaagg aacagatgat tttccttgga tctttgacga
     2041 agaaaaaggt caccggccta ttagatatat cgaaaaaaaa tgtaaaccaa ctgaaggcga
     2101 ctttggttcg tttgttttgc aaccttggca gcatttcata attggatcca tgtacggatg
     2161 ggtacatcgt gatacaggag agcgtcgctt ccgcgaggct cttatttttg ttggacgtaa
     2221 aaacggtaag ttttgccgtt taaaaatcgg gcaaaatcgg tgaaaacctt tatttttggc
     2281 tttcttttag taataattcg atagctttat caagcaattt agacatagga accatagttt
     2341 cttccgacat tttttttaat ccttcgtata aatctttatc aattgcgtta gaaattctaa
     2401 ttctgttttt tagtccgtat ttgtccatgt gtgacactcc tttttatttt actataacat
     2461 ccgattccac cgcttgcaac taccatcaat tgatggtagt atataaaaga ggtgagtaga
     2521 ttgaaaaaat acattattta caaacactta aataaaataa atggcaaaat ttatatcggt
     2581 gtcacaaatg atattggcag aaggtggagg agcggtggga ttgaatataa accaccaaag
     2641 aacgaaaccc aacaccacag aagtttttgg aatgcaatac aaaaatacgg gtgggaaaat
     2701 tttgatcatt tgattattga agaagactta acgatgaaag aagcttttga gaaagaaaag
     2761 ttttatattg aactttatga ctcaactaat aagaaaaaag gttataacat cgcaaaaggc
     2821 ggaaatggcg gaataatata taaagttcac cccaaaggaa tgctagggaa aaaacaatct
     2881 aaagaatttt cttcaaatca cagtaaatgg gcaaagaacc ataagaataa ttgtatgaca
     2941 aacggtgatg ttgtatgggg tgttactcac gaacacccaa aaggcatgtt gggaaagcac
     3001 cacagcaaag aatcaattat gaaaaagaaa gcatatagtg gtgaaaatgc tgtaacctca
     3061 aaaccaattg tcgcaattga acgggatgga actaaaagag aatttcacag cgctaagctt
     3121 tgcatggctt actattcgat tagtacatgt gttttttacc gacttttaaa agatggcgcc
     3181 ccttatgtga taaatcctaa agcgaactac agaaataaag aaaaaatatt agctatcgaa
     3241 ggaattatgt tcaaacataa agaagatacc gaggtaagtt agtagattgc gaaaggttac
     3301 taactaccgt aacgcgtagg agttgaataa atataatgct cccaagagtg tccgacagca
     3361 gataatttgc tgaaaatgta cgctaaactg ggccagaact gactgaccga tgaaaatgag
     3421 ggtgacctcc agagtgcgag ataaaaagct cgcagataat aacaatcgaa aacatcacta
     3481 atttcaggcc tttccacata catggtcgct tatgatgatg aacaaggcgc caacgtttac
     3541 gtattggcaa atgctcgtga tcaagcaagc ttgttgtttg ataaggccgc agaaatggtc
     3601 aaacaatcgc cggcgctctt taagaaattt ggtaagccta aacgatcaag tattaattat
     3661 gctcccgcct tttctaaaat ggaaccacgc gcctcagata gccggaaatt ggatgggcta
     3721 aacactcact ttggtatttt tgacgagatc cacgagttta cgaattacaa tctgatcaac
     3781 gttatcaaga aatcaagagg aaccagaaaa cagcctctga tagtttatat cacaactgct
     3841 ggatatgtat tagatggtcc gttgatgtct tattttgagc aaggtgtgga ctgtttggaa
     3901 catttggaag atgacatcga tgaacggact ttctattatc tggcaaaact tgacagtgcg
     3961 gaagaggctg atgacccaag attatggatc aaagccaatc cgaatatttg tctaatgaat
     4021 tttgttggca tgctagatga ctatgttaag gataaaaaag atccaaaaga atatgctgac
     4081 tggattacca agcaatttaa cttgttttcc gatatcgatg agctgtcatt tgtcgatatg
     4141 cctaccatta aacgaaacaa taaaaccatc gatattgaaa cgctcaaagg taagaagtgt
     4201 gtcggtggtt ttgacttgtc cgaaacggaa gactttaccg cagccgtttt agaatttccg
     4261 cttgaaacag gcgaggtatt cattttgcaa cacacatgga tcccacaagc tagatttgat
     4321 cgagataaca atcaagagcg tatcaaagcg tgggagaagg tgggagatct aacgattatt
     4381 cctggtgatt acgtcaatta tgaatacgtc ttaaattggt ttgtagaaaa ttcgaaaatc
     4441 tatgacattg taaaaatcaa ttatgacaag gccaaggcgc tacgattaaa taaagaacta
     4501 gaaaatgcag gatttgaaac caacgagatt cggcaagggt ttctatcatt aggtgggcca
     4561 atgcaaaact tcaaggaaat gctattggac ggtaaggtga ttttcaacaa ttccaagctt
     4621 taccgatggt atctatccaa cgtcaagctg gtgatggatc gcaactcaaa ctggatgccg
     4681 tctaagcagt ccaagagtag aaaaatagat ggttttgcag caagtttgaa cagccacgcc
     4741 gaagtgttga atatgttggt taatcctgtc ggaaccggga aagtaaccta ttactcgatt
     4801 tccgatttaa tgaatatgta agaaaggtgt ggaggaatga gtattttaga tcgtttgcgt
     4861 tcttttggcc gagcgaagcc gaaagcgagc aaacaagagt attttttgaa tgacccggga
     4921 ttgataccgt atttagtcgg aaaagatgaa atatcagaag ggattttttc cgtaattagc
     4981 cgtgtatcga acgtttttgc gtctctccct ctcaaaatga tagatgcgga gtttggccaa
     5041 ccggacgact gtcctgcata caacttgttg agcgaaggcc ctcgatattt tacaaagttt
     5101 gattttttcc gggacgtgga agttttgaga aactaccaag ggaatgcgta tgtgcagatt
     5161 ttccgaaata tcaatggaga agtagcagat atggcgttaa taaaacctgg tgcttgccat
     5221 ccagtgattg atatggatag cggggagctt tactaccaag taactgcgac tgacaaaggc
     5281 agttataagc aagttattta tgtacattac atggaaatgc tccactttaa acaaccgagg
     5341 tttggtggct tggaaggtac agaccccaca aaagtattaa cgaataccct cggatatgat
     5401 cgagaagtcc gaaaaatctc tttaagtcag cttaaaggag gtaatgaagg gctaaaagtt
     5461 aagtttgcta gcaatgtgga tgaagaagct aaaaaagcta cagttaaaaa cattgctgat
     5521 ttttatcgac aaaacggtgg actacttgtg gaagaaaacg gtgtagaaat cgaacgttta
     5581 caacgagagc tggtagacag caagctttta gatactgata aagtatctcg ctccagaatc
     5641 gcgatggtct acaacgtgcc ggaacatttc attgggagta accagtcgag ttactcttca
     5701 caggaacagc tcaatatgga gtttttgaca tacaatctag taccgaccgt taatcaatat
     5761 gaagcggaac taaataagaa aacactatcg agaaccgaaa aagcaaaagg ttatcgatat
     5821 aagtttaatg tcgcaagttt gctaagagct gacactcagg caagggggca attctatcaa
     5881 atcatgcgac gaggcggagc atactcagcg aatgatgtcc gaagatttga ggacttgcaa
     5941 ccgataaata aaaccggtat ggatgattat catatttccg gagacctata tccaatcgat
     6001 atggatccaa cattaagaaa aacaaactcg tctaaaagcg tagccgaaaa cggttaggct
     6061 tttttagttt gcaccgaagg gaggtgaaag gatgaaaaaa gtgacgttaa gcggcgatgt
     6121 cgtggataac gataccgcgt ggttatatga ctggtttggg attgattgta tctcaccagg
     6181 gaaaatttct gccgctctta cagaagcagc gggggatgaa gtagaacttg atatctcatc
     6241 taacggtggg gatgtcctag cggcaagcga aatatatacc gctatccgtg cctatccggg
     6301 aaaggtatcc ggaaatgttg tgagtattgc agcaagcgct gcaagtgtaa tcgcttgtgc
     6361 ttgcgaaccg cttagaatct cgccaacagc acacatcatg attcacaacg catgggtgac
     6421 cactagcggc aacgccgagg aattaaaagc caatgcggaa atgttaagca gtgtggatga
     6481 gtctatcgtt aatgcttacg agatcaagac aggactcgac cggaaaaagc ttgctgattt
     6541 aatggcaaaa gatacttggt taaacgcaca aacagcagtg gcggaaggtt ttgcggatga
     6601 aattatgttt gcagaagcac cagtaacggt actcaatgcc tctcaaccgg ttattccaaa
     6661 aaacgcggta actaagttga aaaatctaat actcaaagcg gaaacaccgc aagaagaaac
     6721 gctcttgcag aaaaaactaa aagccttaaa tggagggaaa aacgaatgaa tttagaacaa
     6781 ttaaaaaatg cgtgggtcga ggcgggaagt aaagtctctg atttaaacgc gcaactcaat
     6841 gcagcattgg ttgacgatga aaaaacagaa gaagatgtag taagtttgca agcacaagta
     6901 aaagcagcac gggctaaacg ggacggattg aaagagcaag tggcaaatat ggaagccgaa
     6961 caagtcttaa acgtcaaaaa agaaccatta gataaaaaag atgaaaactt gaaaaacaag
     7021 tttatcaaag actttaaagc gatggtcaat ggtgatcctg ctattatggc tactttgaca
     7081 tctaatacgg atgaatctgg taatgctatc ggattgacta ttcctgtaga tgtgcaaacg
     7141 actattcata ctttggttcg tcagtttgac tctttgcaag aatacgtaaa cgttgaaaaa
     7201 gtgaccacta ctagcggttc tcgggtttat gaaaaatggt ctgatattaa cccactgacc
     7261 gctttggata ctgaagacgg tgaaatccca gcaaatgatg atcctgcact ttacttgatc
     7321 aaatacttga tcaaacgcta cgcaggtatt tctacagtaa ctaacagctt gctaaaagat
     7381 actgccgaaa acattttggc atggttgtct aactggatcg cgaaaaaagt agttgttact
     7441 cgcaatacaa aaatcttggc agctattgat ggaatcaaag cagcgcaaaa gaaagatgtt
     7501 acagatgttg atgggattaa agatatcata aacgtccaac ttgatccagc tatcgaagct
     7561 acatctatgt ttattacaaa ccaagatggc ttcaatgttt tagataaagt gaaacgtgct
     7621 gatggatctt acttgttaca aaaagacgta gcttctgcaa ctggatatac cttcttaggt
     7681 aaaccaatca agaaaattgc ttctcgtttc ttgccaaaca aaggtacgca agctactcct
     7741 aaatatccac tgtatattgg tgatctgaaa gaagctgtta cattgtatga tcgcgaaaac
     7801 atgagcttgc tgacaacgaa tattggcggg ggggcttttg aaacagacac caccaaagta
     7861 cgggttatcg atcgatttga tgtgcagtta gtcgatgatg aagcagttgt tttagctaca
     7921 tttacatcta tcgcaaacga agtgccagta acaaacggtt aaggagctga tttcttatga
     7981 ttcttgatcc taaaatggat ttagacgaaa tcaaaaacgc actaaagatt gataccgatg
     8041 atgacgatgt ggaagtaaga cgtgcggtgc aagctgcaat tgcatacatt agaggggcta
     8101 tcgggaatga taagccctct ttttataagc aagaaaatga cacggttgat ctgattaatt
     8161 tagctattct gcaattagca gatcattatt acaacgctcg atcagcaacc gtgagtggga
     8221 acttgcgaga gtacgattta ggttttacaa gcctaatctt gcaactcaaa gcaagatatt
     8281 tgctttttgt ggaggagtag cgtatgcccc ttattcaaac aggaaattta aatcaacgca
     8341 tcaagtttgt ccgagatacg acggtcaagg acgaggacgg gcaagttgtc cggacttcta
     8401 caaccattct tacttgctgg gcaagtgtgc agacacaacg cctgaacgat attaagacgt
     8461 ccattggtac ggctttggaa ggaacactga cgttcattat ccgctatcaa caaaaatcag
     8521 agctaaccaa tgatatgaaa gtgcgttgga atggaaaaac gtttgaaatc attacgatta
     8581 cgaaaggcga gtttgcgaag gacttcacga caatcattgc aaaagaggtt tcaaaatgag
     8641 tgtagaagtc gatgcaaccg aagtgtacaa agcgcttagg gaagtaaaag caaacgttca
     8701 acgagtggaa agcccagcac ttagaaaagc tggggagtac gctcaagaaa agttacaaca
     8761 aaacacacct tactgggatg gaacgaagtc aaacggtaaa cgtggttcgt atatgcaaga
     8821 acatgctaag gaccatgtgg ttacaagctc ggtaaaaaac ggattgatag aagtcggcta
     8881 tgataaagat gtttcttggc ggatgcactt tatcgagttc ggaacaatca aacaacgtcc
     8941 aaaaggtttc gtacaaaaaa cacaaaagca aatcgaaaaa caagtaacac aaatcattgc
     9001 tgacgaagta aaaaggaggc taggactttg aaaacggcag tatcacaagt ctattcaatt
     9061 ctgaatagca atgaaaaaac aaagaacatt gatttttaca ccaatagtgt tccggaatcg
     9121 gctcaaacag tacctagcct tccagttggc agaattacag agatattcgg caactatgaa
     9181 gatttcgcaa gcaacaatcc tttgaccatt caatttaacg tacaggtaga tgtatgggta
     9241 tcgaccttaa aagaggttga tgccttttat tttgcccttg atgagattat gagggggaat
     9301 ggttggcaat gcgcatacac ggaacaaaca gatgatgagg atttagaagg tgcaaagcgg
     9361 attatcaaac gatatgtagc aaatatttca ctaaattaaa aggagagaaa atagatggca
     9421 acagtaggat ttgagagcgt catttttggc gtaaaaacag gtgcaggcgg cactctaaaa
     9481 gaattagtag cagataagtc gaaaggcgga gcgatcgaag ctaaaattac tggattaggc
     9541 gcaacttcta acacaacata cgcttcaaac gtaccattct tcattgcaag taaaggggtt
     9601 tcgtcgccaa aagttacgct tgacgtggca gacttaatgg ataacggcat ttacagcgaa
     9661 atcattggcg ctaaaaccgt ggatggtgta aatgtaattg gttcagaaac tgaagcgcct
     9721 tacgtgtcgg tagtcatggt tacagcgaac aaagaaggaa aacgcttatt catgggattg
     9781 gcaaaaggaa aattcagtca tccagatatc gacatgaaaa cagctgaaga caaaggagtt
     9841 gaattgcaaa ccgattctat cgaaggggaa ttcatttctg atgaacgtgg ctatgtatac
     9901 ttaacagccg tagaatcaga agaaatgacc ttacaaaaat tcaaggactt ggtaaataac
     9961 aaagcggggg agtagttaac cctgcatcta caccaacgac agatacaggg acaccaaaag
    10021 aaccagaacc aaaaattgat acacaaggtt agccattttt tggctagcct tatttttttg
    10081 taaaaacaag gaggaaaaca aatgattgaa ttgcaattga aacttgacgg aaagaaaaaa
    10141 acattcaaac aacaagatat ttccgcacgt gcaatgcgtg agtgtatcaa attttacgag
    10201 aaagcggaaa aagcagacct aactgattta gaagcaattg attcaatgat tgcaattaca
    10261 gcagatattt tccaagatcc agcagttaca tttgatgcta ttttagacgg tttgactgcg
    10321 agcgagttag taccggcatt agaaagtgtt tttgaacaaa tcaatgaact gggaaacaat
    10381 gaaaaaaagc agacggcgag caaaaagaga taagtttttc tgaagctagg aaagcaatgg
    10441 atcaaatcta caaagattta atcgaatcag gttggacgat gagagatgtg gacgaagccg
    10501 actatcatta tttgttacac ctttttggag aagtggagag tggcgaagaa tatgtagatg
    10561 gtgctgattt catcaaacaa tttttatcgg ctgaagactt ggtaaaactt gaggaaggag
    10621 gtaaataatg gcaggaaaag gacaaccggc aggaaatatc aagctaggga ttagtttaga
    10681 tagcactaat tttggtaaca cgctggacga aatcaatgcg aaagtcaaac aagctgagtc
    10741 gaatatgcgt gccaatctaa aggcttatga ttcagcagga cgttcatacg aagcacttag
    10801 tcaaaagacg aaagacttgt ctacggttat ggaagggcaa aacgccaaag taagagaatt
    10861 aacaaagcgc cgtgatgaag cgattagcaa gtatggcgag gaatcgaaac aagttgctaa
    10921 ccttaacaca cagataaaca atgctaccgc aaaatataat gcttacagtc gccagttgaa
    10981 tgacacaaaa aaagaattgg tgtattccaa aacagccgtc aatgatttat ctaacgaaat
    11041 caaagaaaat gaacgacaaa tgaacgccga agtaaaagcg ttgaaagccg ctggtgatga
    11101 atctggtgcg tttgaagcaa aacaaaaagg gctagccaaa caaacggaat tatccgagaa
    11161 agctatcgaa gaacagcgca aagttgtgaa actgatggct gatgagtttg gcgattcagc
    11221 aaatgaaacc gaagatgcaa aaagggcatt agaaaagtta gaacgacaaa gccaaatatc
    11281 tagcaggcaa ttagaagcac tcaaaagctc cagcgatcaa tcaggaaaaa aaatagaaga
    11341 ttttggcgac aagtccacaa ggtcagctag gaaactggat gggctaaaag acaaattagg
    11401 ctcgctaaaa agcgcatttt cgtttggtgc agttgctgga ttagcgcata acgctattag
    11461 cagtgtagta agtggcgtgc aaggcttggt tggcgaagca gtaaacgcat cggattcatt
    11521 gatgaagttt tccaaaacca tggagtttgc taactttggg aagtcacaga tagaaagctc
    11581 gaaaaaagaa atgaaagact acgccgataa gacggtttat ggtttagaag aaattctgaa
    11641 cacaaccgca caattggcat ctaacgggat tcctaactat acagaactaa ccaaggcggc
    11701 aggtaacttg aatgccgttg caggcggttc tagtgataca ttcaaatccg ttgccatgat
    11761 gctaacgcag acggcaggag ctgggaaact aacaactgaa aactggaatc aattagcaga
    11821 tgcgataccg ggcgcttcag ggctgttaca agacgctatg ttgaaaaacg gagcttatac
    11881 aggaaacttc cgtgatgcaa tggcgcaagg tcaaatcact tctgatgagt ttaaccaagc
    11941 gattgtacag ttaggtatga atgacggagc agttaaagca gctacttcca cagatacatt
    12001 gagtggttct tgggaacaga tgaaatctac tgtaataaat gggttacaaa gtatcataga
    12061 caaagtaggc gttgaaaata tcactggttt catcaacaga gtaacaaaag ggattgaaaa
    12121 ttctattcct aaaattactc aatttatagg ttggttgaga gatattggaa cgtggatcgt
    12181 tgaaaataga gagccactaa catggattgt cggaatcata ggcggaatta cattagcagt
    12241 aaaagcattg aacgtagcaa gtatgttgct ggcaattact ggcggaacat tggcagcccc
    12301 ttttgtggcg attggtgtag cattaggcgc actagcaggt gctttggtga tagcttatac
    12361 aaaatctgaa acatttagaa atatagtcaa tgcggctttt acagctgtga aaaacgtagt
    12421 tatgagcgtt atcaataatt tggtggaata ctacaaaatg ttgtggagcg tgttgcagtg
    12481 gctttgggaa aaaataaaag aatgggcttc atggattggc aataaattca ttgaaatgaa
    12541 gaacagcgtt gtgaatacgg ttcagaacct atggaatggt gtgaaaaact tcttcagtaa
    12601 tggcgttgga gacacttgga ataaggtagt tggctgggta aaaaacattt tcaacaaagc
    12661 aactgaattg aagaacaaag tttctgatgc aattggtaac ctgtggaacg gtatcaaaga
    12721 cacattccgt agaggcattg atacggtatt caattggttt tcagaactac cgacgaagat
    12781 gaagaatgcc attattggcg gtaaaaatgc cattgttgat gcgttcaaaa gtattttcaa
    12841 cgcagcactt aaagcgatag gtaaaccagt taacgcaatc atccatggag cttcatgggt
    12901 actagaaaaa ctgggtgctg acaaactcga agaatggaaa gtgccacaat acgcaaaagg
    12961 aacaccaaac ggaggtcatc cgggcgggcc tatgatggta aatgacggta gaggtgctga
    13021 agcggtaatc acacctaacg gacaagcatt tatcccacga gggcgaaatg tagtgttgaa
    13081 tgcaccaaaa ggcacacacg ttctaacagc tgaagaaaca gcttatatga ctggaaacaa
    13141 agcaccaaga tatagatacg ccaaaggtac aggctttttc ggaaatctat ggaacaacgt
    13201 caaaggattt gctggagatg ttggaaacaa gctgaaagat gtagtcggcg atgtatggga
    13261 ttttgtaaca gacccgggag cgttggctag gaaagtgtta aatggtcttg gcgtactgga
    13321 agggcttgtc aaatatcctt tagatgttgg taaaggtatt ctaagcaagg ctaccgaagc
    13381 attgacgaac aaaatcacag aactattcag cagtggcagt ttagacactt caatgggcat
    13441 gcaaggcgtt tacaaatact tggcggacgt tgcagttgca gtaatgaaga agtttccagg
    13501 ctttgtggca actagtgggt atagaccagg tgacccctat tcgcatggta aacgtaatgc
    13561 cattgatatt gcactacctg gtgtcacagg aggctcgcca cgctacacag aagcagccaa
    13621 ttacgcattt gagaagtttg caaacaaaat cggctatgtt atcacaaatg gcaaggttcg
    13681 tgaccgttca ggacaatcag gtacaggtat tcacaatgat tggagaccat ggcccgatgg
    13741 agatcactat gatcatgtgc atttaaacgg tgtgaaagac ccacaaaaca ctcaaatttc
    13801 aggagatagc gtgggaggca gtggggtaga aagatggcgc aatgtagcaa ttagagcgtt
    13861 gaaaatgacc ggtcaataca gtactgcaaa cttaaatgca ttactaaatc aaatgcgtac
    13921 agagtcaaat ggtaatccta atgcagttaa caattgggat attaacgcca aaaatggaac
    13981 accatcaaaa gggttgctcc aagtgattga cccaacattc agacagtatg caatgccagg
    14041 attcaacagc aatatttttg acccactatc taacatctta gcttcaatca gatacgcact
    14101 atcaagatat ggctcactaa caaatgccta tcgtggagtt ggttacgcaa acggtggaat
    14161 tgtaaaccaa catcaaattg cggaaatcgc agaaggaaac aagccagaaa ttattattcc
    14221 tttagataag gctaaacgat caagagcgat gcaattattg tctatcgcaa tggacaaact
    14281 aggagtttct cctaaaactt atggaaatgc gacaactgtt tccagtgatt acgaaatcct
    14341 agaagccatt gaaaaacagg cggcaacaac gaatcagctg ttatcgttat tgctggcatt
    14401 tttcaaagga aatagccgaa ctgatagaga tttagcttta gatattcaga agatcttggt
    14461 taggaggatg tagatgcgaa ctgtattact gagaaataga ataaacgaag aaattgattt
    14521 gtctacggag gattattttg ctactgggtt gagtaatatg gggtttgaag taaaaaaaga
    14581 acatgtggga cagtggggga atttcagaga aagcagtgag aacgttgaaa tatctgaatt
    14641 tcagtcatct gtaatcattt ctgtgcatgg gtttcgagaa aaagaactgt acaattcact
    14701 tgtgcagttc ctatcggaag gtccgtttga actggaattt gcttttgacg gcgaaacaat
    14761 ggtaagaaga tgcagtctaa aatctttaag taagactgaa atcgatccga aaacatcgtt
    14821 actaacagac actttagaac tatatttcac atcaaactgg tattccgtaa aacgcgaaaa
    14881 attaattcag cgaccgaatg tagtgaaaac acgtggtaag gtttttccgt ataaaagatc
    14941 ttatatctac actcaaaact tgtgggagaa aaaaggtgta ttcaaattca ataataattc
    15001 tgtataccta acgaatagca aggaacggat gtccccgcta aaaattcgtg tgattgggaa
    15061 gtgttcaaat ccgtattggg aagtaatcca aaactcacaa atcatcgcaa cggatggata
    15121 cttcatagat atgactgaaa cacagactct agaagtatca agcctttttg aagatacaac
    15181 ggcgatttta aaagatattg caggggtaga atcttctgtc taccaacaac aggattatac
    15241 aaaaactaat tttgttcagg ccccgactgg agaattcagc attgtgtttc atgttggggg
    15301 ggcagacgta gagattgagt tatacgagga gcgtgatctg ttttgatttt agcagtcacg
    15361 ctttttcatc gtgatttaat gctttataac gaacatttat tctctaacag ttttgaattt
    15421 ggtgcggacg agatcaacga agaggctagt agctttacga tggataagta cgttcctgta
    15481 aaaacaggcg attttctact agcaaaatat attcctagtg gaaaatttgc atattttggt
    15541 gtaattacgt cgcaggaaga cgaaaaaatc agttgtaaaa gcttacttag tttagctgat
    15601 agtgagatac cgactgcacg tgtgtcagga gataactacg aagagcatat ccggcggttg
    15661 attgaatact atttgctgaa tgatccaacg aaacaactaa aagatatttt agacgtcaaa
    15721 gcagaaagtg cgacctctca ttcgtatcaa gctaccgata cgaataaaca caagttaagt
    15781 gcgtatattc tcaatggttt caaaaaatac aacgtaaaat ggtatttcaa gggaattcaa
    15841 aacagaaaaa tctatacagg catacgtgct gtaaatgaat caatttacat taaagacaat
    15901 tcttctgaat ttagcgattg ggatgtgttt gttcaggcgc cgggcgctgg aaacgagaac
    15961 aagctattaa tcgttgataa agcaatgaaa gatatagaga aaccgataat actgtcaaca
    16021 tggtatttgg acgaagaaaa caatttgaca caagatggat cgaaagaaaa tatcacgaaa
    16081 ccaactgtca atttggttaa tatctacgat caaaccgcag aagataaagc atcctacgaa
    16141 gatgtggcaa aatcagagtt gaaaggcaat acgtattcgc atgaaatcaa agtgaacgtt
    16201 gtaagaaatg caaaaaattt gaatgtcgaa acgattgaaa cagggatgtt tgccacgatt
    16261 tcttataaag gaaagatata caagtcggtt ttgacagctt ggcgaatatc aagtgataag
    16321 gagtttgtgg aattgacttt cggaaacata agaagtcgtt ttatggatta ttttgaagat
    16381 aatgggggat aaaaaatggt tagcaatgtg gatggatatc aatttgaaaa cgtgaaagta
    16441 agcgcagaaa atgatgctag actttatcac gttttatata atcggaaaaa tcaggttatt
    16501 gatggttacg atcagtctat gaatttatct tcaagcggat taacagtaaa agttgctgca
    16561 ggagcagcga ttattcaagg tcgtatggtc gttgttcgac aagaagaaag tataacagtt
    16621 ccagcaaact caagtggtta tatagcatta acagtggatt tgacacaaga agttatacct
    16681 ggatctattc ttccagaatc ggaagaatat gaatggacta ataatcaagt caagctagaa
    16741 tttataacaa aagttataaa aggtaattta aacaatggtg ataaggtcta taatttacca
    16801 ctatgctcag ttacttctac tggatcaact gtttcaatct caaagatatc ggatagttac
    16861 gagctgactc tctctaaagg agaaattttg tggagtggga ctgcgttaat gcatgatact
    16921 caaactgtcc aaccttcaaa aaaaatgtgg cagacagttt caggtttttt attagtatgg
    16981 ctcccatacg aaaacgggca agcaattggg gatagatatg taactacgcc tttttataaa
    17041 gagcgtgtaa cttttactaa tgttttagga gaaattgttt caggatttga tgactatcac
    17101 aaaaagtggt ttagtaaacg aataaactat aattcgaata ctaatatatt tacaggtgca
    17161 gcaagcaatg caagtggaga taacgcaaat atggtgctta ggtatatagt ttctttttag
    17221 ttaggaggta tggaagagtg gcaaatttag aaattaaatt atctgcaaat aagagacagc
    17281 cttatctacg tcaccgtgtt gttggtagag ttggtgatgg ggggctcaca acaatcaatg
    17341 tacaacttct tgaggaagat gaaattacac cttttgtaat taatctaaac ggtactttga
    17401 aatttgtggg cgaagtttca aacggtaact ataccgaggg agaaccagaa ataatcgatt
    17461 cgactaatgg gttaattagt tacacgttca ctaagtcaaa tttcagcacg agtcatcaat
    17521 tcaaacaagc atattttgaa tatgtagatc ctaacggcaa aaaagtaact tttcagaact
    17581 tcatcataga cgtgttagaa cgagtagata ttaattcgga gcaagcgaaa tactatattt
    17641 cttcattgga aaaattacag agtgaaatgc aaaccacttt caatcagttc attagtgata
    17701 aacaggtcca atacgatcaa atctactcga aatataacga attagtaaga ttgataaatg
    17761 aatcagataa gcaagtaaat gatcgtattg ataagacaaa tgatcgtatt gatcaaacca
    17821 atcagcaaat cggcgatctc ggcaagctga aaaagatgta cagtaacagc atcgacttcg
    17881 gggactatga ttatagtggg aatccgaacc ttagcgctaa acttaatgct tcaagttttt
    17941 ctttaggtac tggcgcaaca gtcgcggatg ataatgatga gattgtgttt accttagatg
    18001 gcacaaatca actatcgaag tacgctagca aagcgcaagt gccggtagca gaagggaaac
    18061 aatatacgat tagttgtgaa attatgttag aagatgggtt cactggcgac ccttcaggaa
    18121 tacgtctgca acacgcatat ttgccaggcg gggttgttgt tttacagaca gagacagtac
    18181 ctaaaaatga attgaatacg tggcagacgc ttatcggaac gcgaacggta aaatacacct
    18241 ctgacgtacc aaatgaatgg taccctttgt ttagagacat ccgaacactt aaaccgaccg
    18301 gtaaagttag actaagaaaa attaagattg aagaaggctc aacagccacc ccatatcagc
    18361 caaacttact tgatgaccct tactggctag gtaaagcgcc tttgggtgag aatattgcta
    18421 ataagtctgt tacgtttcca atcaaatcta gcgcctacag cctataccaa gctaacatgg
    18481 aagaagaatt tgttttaggt caaacctata caattaccat gaaggcaacc aaaccaccta
    18541 tccaaacgtt cattgtttac aacgaggata gtggagatta tagatatggt aacttagagc
    18601 cagtagaagg gttagttgat acatggggtc tgacttttac accgcagaag gttggagtta
    18661 actaccccaa acggttaact attatccagt atccccaatc aacaacaggt gcatgtcaaa
    18721 ttgactggct caagatcgaa aaaggcaata ctcgaacccc gaatattagt gagtataaat
    18781 atcgtggtac tggtatgcgt gattcaaaca atccaaaaga ttatgtttgg gatctagcac
    18841 cagaatatgt cgaagataat ttggccacag atattaaaat ttctgaaatt actggtaaag
    18901 caaacaatta taccgatggg aaagtatcgg agattaattc gcagttgact gcttcaatta
    18961 atgaagtaga caccacagct aaggatgctc aaacaaaagc gaatgctaat gcgactgcta
    19021 tagatgaatt agacaataag atcgatgaac gcattaatga tacagctact accacattaa
    19081 cagttacaaa cgggaatacc ggatcagcaa agctttatcg tgaaggaaaa acagtttcta
    19141 tatattttgt ggctttaaac ggaaaaagca gtggtggaaa tgattcaacg atactaacaa
    19201 ttccagaagg ctatcggcca ccaattagtt ttgagcaact ggttggctcg atagaccgtt
    19261 ctactttgaa cagtgctcag ttatctattg gtgcagatgg agccattaaa tggcgaagaa
    19321 actcaagtta tggatcggat tatacctttg caattactta cacgatttag aaaagcgtga
    19381 atcgatatga aggcagcata tagaccaatt gaaccttacg gattcgagca aatcattgtg
    19441 aatgatgaag aacatttacc ggaagaatgc acagaagtcg aaccaccgat tctaaattgg
    19501 aaaccgaaat tcaattactg ggagggaaat aaatgaaaaa catttggaaa tatggacgta
    19561 ctggcggaga gtacgcagga aaagtattgg acgacatgct tgtatccgtt ccttacacag
    19621 atcagccacc gcttgaagga attcgtgctg atggcgaacc gttaacaatc gctgatcaaa
    19681 tgtttgatcc taaattgaat caatggatta ttttagcgaa cgcactagat cacaacgatt
    19741 taaacaatct caaagcaatg tatgagtcgt tagaaaatga gaacggcgat ttaaaacaga
    19801 tcaatgccaa actcatgcta agcgatgtag caattaaaca ggaaaatact gcattgaaag
    19861 aaaaagcgga tagtttagca caaatcaatt caaaaatgat gcttgcttcg ttacaaaata
    19921 gcaaagacat ttcagaaatt aaagagcaac taaatccagc ttcaaaggga ggtgagtagt
    19981 atgtttagtt ttagcgatgt gaaaatgatg tatgattggg gctgttttac tgacgatcaa
    20041 gttcgactat tcgttccact atgcattaca gacgaagaag cagataaaat cattagcaaa
    20101 gaagaaagcg catcttaagt gatgcgtttt tatttaaggt aaaggagttg tcacatgatc
    20161 aatttagggg aatggggaac aatcgcagga tcaattactg cgattgtttc tttgatttta
    20221 ttagtaataa gaccagtcac tgcatctttc tcgaagatta ctgaaactct ttcaaaagta
    20281 agtcacaatt tagatttgct gactaaagat ttagaatcga gcaaatcaga tcgattgatg
    20341 attcatgaag aactaaagaa acacgatgaa agattagata cacatgcaga aaaattggta
    20401 gaacacacac aacaaattaa aactttattt agagaaaggt ctcggtaaaa aagaaaggag
    20461 ttaagaagaa atgattttac ccgataagta ttatcaagtc attaaatgga cagttttaac
    20521 agtattgcca gctttatctg tattagtagc cacattaggc aaagcatatg gatggaatgg
    20581 aacagatatg gcagttctga ctatcaatgc agcagcaaca tttttaggtg ttatcactgg
    20641 tgtgtcggct tataatttga aaaaatagga ggaaacaaat gaaaaagaaa attactatta
    20701 ctgcgatgag cctattaacg gctctttttt tattgccgat taatgggttt gcctatacga
    20761 ttaacaatga atttaattta ggtgtaaacg aaggtagttc tcaagtagca aataatcagt
    20821 acattttact gcatgaaacg gctaatgaaa cagcaacagg acgcaatgaa gcgcagtata
    20881 tgcaacgttc atggactagc gcttatactg cttatattgt gggagacggc ggaattgttt
    20941 atcaagtcgg tcaacctggt tatgtacagt acggtgctgg ttcgtatgct aatgcaaata
    21001 gtcctgtgca gattgagtta caacacacac atgataaagc aacgtttgag aaaaactaca
    21061 aggcatacgt tgaattagct agagattcag caatgaaata tggtattcca cttacactag
    21121 acacaccgta taatcaacca gggatcaaat cgcatttatg ggtaacacaa aatatttggg
    21181 gcgatcatac agatccttac ggttatcttt ctgaaatggg cgtaagtaaa gaaaaattag
    21241 cctatgattt ggctcatgga tttaccgatg aaaatccgac aacttcagat gataaaccag
    21301 tcattgatcc aactcgagca ggtgctgcaa atcctaccct gacagatgga acaaattacg
    21361 cccacattga tcagttcgga gaaatcgaaa acgcaaactt acatgtggct ggttggcaca
    21421 tcgctaacta taaatacgag tatatcttca ttatggatta caatactggg aaagaattag
    21481 ctcgagtaaa agctgatgga atatatagac cagatgtaaa tcaagcttat aatactttag
    21541 gaaatgttgg ctatcatgta tcttttaaca tgcgcaactt ccctaataag aaagtctatg
    21601 taatgatgcg ggcaacgaat gatccagaag ggaacactaa aggcggagca caagattttc
    21661 atgataaacg ctggtattta aatattccgc aacgataaaa atagcccctc gtttttgagg
    21721 ggaagtacat aatgcaaatt tgtccataac ttattgatta tcttgtaaaa atgcggtaat
    21781 ttaatagtta gtgagaagag gtgcttaatt atagttaaaa aagaggaaat agaagcagca
    21841 tgtaaccaag caaatgttag gtttattgga gaagctttat ctggatatcc taagtataaa
    21901 tacttacaca actggttaca aagtaaaagt ttttatctta ataaagaaag atgcatgcct
    21961 aataaaaatt atagggttta taaaagagga actattgtat atgttgattt tggggtgaac
    22021 gtgggttatg agttatctgg taatcatttt gctattgttt taaataataa agataattcg
    22081 aaaaatggtg ttgtatgtgt agtaccaatc tcttcaaaaa agaaaagtaa ttatgtatct
    22141 gtaggaaaaa tattagaagt tgctagtatt aaacaattta taagccaagc tgataaatta
    22201 aaagagaaac ttaggataat tattttactt tcattaaata gaggcatcat tgatgaaagc
    22261 gatttgaata gttttattaa agatattgaa gctaaaggtg aaaagctttc agtggaagaa
    22321 gtagaaaaac gagctgtaaa acataatttg gtttgcgaga caagggaaca agtaattaaa
    22381 gaaataaaat cattatctga agatatggta aaatatcaga aggtgtttaa ttgctacaag
    22441 aaatatacac aagaatccta tgtaatgcca cttaatatcc aaacgattag taaaaataga
    22501 attagaagaa ttaacaagtt tgatccttct ggaaatatga aggcacctgg aaatgtcctc
    22561 aatgaagtag atgaaagttt aaaaaaatat tttacaaagt aaacgatatt tttgttgaca
    22621 tttttctgtt cgttagttac catgtagtta tagaaggacg taagcgccta tttttaatgt
    22681 acacatcggg acgtatgcgc ccacatagaa acatagttga gagacatcta tatggtgtct
    22741 ctttttttat taatatgcaa aaaagtctag ttttttcaat aatatttatg taataataaa
    22801 ttagccacca caacaaagaa tgaaatccat tattatctag tctagtccat tctttttgtt
    22861 tgcagtattt gtgatggctt tccgtaccct tagctcagtt ggtcagagca gacggctcat
    22921 aaccgtccgg tcgtacttct cgtaatccag gaaggccgtc agctccgtcc gcagtagcgt
    22981 gtttacggcc agctccagat gggagcggaa taactcgttc aaatcacctt tggtggctag
    23041 agtctgcagg agttctgtaa taaaatcgtt catagggaag tcctcttttc tgtgaatggg
    23101 tgtggtaact ttattctaca gaagggactt ccttttttct atttacacaa aatattttat
    23161 actctcaaat gtttgtatgc agttacatct gactttattc gaattaagga aaagatttat
    23221 aaaaataaaa taaaaaaata tatatttaaa ttgacttatt gtgaaatcat taactataat
    23281 tagttcagtt tatgttaagg agaatgatga tatgaataag aaaattgtag taagtggatt
    23341 aattataggt ggattagtgt tagcagggat cgcagggaaa aactttgccg atgacatttt
    23401 gttatggggt ggagaagata atattgaaca aatcaatcat aatttggagt cattagataa
    23461 agcactagaa aataaagagc aaaatattag tagtttaaat tcacgattat cttcaaataa
    23521 tcaagaactt gagcaagcaa aggctaacgt tgaagaatat aaacaaaaag tgttgacttt
    23581 ggaaaatgat aaaaatcaat taactataga gaaaacaaat ttagagaatc aattatcagg
    23641 aaaaaatgct gaattgcaag gtaaacaaaa tgaaatcaat gctaaaattg atgaaataaa
    23701 tcaaaaaata gctgaaataa atcaattaaa cactgattgg aataatcgtt tacaaacaga
    23761 acaaaataaa tttaatgatg ctcaaaatac tgttaatagt ttaaatcaac aaattaatca
    23821 attgtcaaat gaaaaaaata ctattaaaaa ccaattgcaa agtactgaac aaaaactaga
    23881 aaaagcgcaa tcagaaaaca gtaatttgaa taattatatt gaaaaactgg aaaaagccaa
    23941 aagcgatgta gaagacacag caaacagatc acaagaaatc gtgactgaac atacaggaaa
    24001 atagatgcat tttttagttt aaatttcttt aacaaaatag tattttgttg ctaaataatt
    24061 tttatttgaa tattctacaa tgatatctcc cctcaaaatg gaggggaagt acatactgtg
    24121 ataatctaca ggtttattga ctgattagga tgttattaaa aaataaaata ctaaaatgca
    24181 tacccgaatt tcatacccga aaaataaaaa atgccaccac gtacactctt ttcgaaacta
    24241 cctaagtagc caagaatgta ctggtgacgt gggtttggca cccaatgggc cgtgaggggc
    24301 tcgaacccgc gacccgctga ttaagagtca gctgctctac caactgagct aacggcccgt
    24361 acaaaaagta cttaataata gtagcactaa gaattttaaa atgcaagact acatttaaag
    24421 aaaatcagaa aaatctttac ttccttatag gaaatcattc atagattatt cacatttgct
    24481 cgctattata taagcatacc aacaaacaaa gttcttgcaa gagaacaaac cctaacactt
    24541 ttttcatttt ttactccttt cccgccgtct ccccggcggg tctcctgttc ttaacgataa
    24601 aagacaagaa ggcttagcct tcttgttttt tgttttcatg gaatcccgct aatcaaatca
    24661 cctagttaaa gcaactagat tgaatgttta tcataagtct gatatcatgt ttatgaaatg
    24721 aaaaaaggag cggttatgat ggaaaaaatg cgtcagcaat taatggagtg ggggaaggaa
    24781 ctagaaacat tccgtctgcc tcactgggaa gaactccctg acttagaact ttatatggac
    24841 caagtcagaa cgttagttga tcgttatctt tcacctgtga tacaaggaga aaagcatcca
    24901 ttgcttacct cttctatggt aaacaattac gttaaactag gtttgatacc agcaccagtt
    24961 aaaaaaagat ataacaaaga acacgtagca tttttacttg caatcactac gttgaaacaa
    25021 gtattgacta ttcctgaaat caaagaaggg atattgttcc aaggaaaaac agtaggaatt
    25081 cgtgaagcct ataatctatt ttgcgatgaa caagaagcag ctgtgtggat ggtcagccaa
    25141 ttagcacaag gtaaatctca tccgcaaaaa ttcgaccagg caacccctgt agaatatatt
    25201 gctgtaagag cggcaacttt atcgtttgca atgaaacttc ttgcggaaaa aacgattgtt
    25261 ctagaaaccg aatatttaaa agaggagaaa acaaatgaaa aacaataaaa tcgcaatttt
    25321 agtagattca ggtacggatg tgccagaaga attgataaaa aaatacgaga tgtatatgat
    25381 cccattgaaa attatttata aagatcaggt atatacagat aaaattgaca ttacctctga
    25441 agagatctat caacgtttgc cagaggaaat ccctggtaca tctttgccgg atggtgagtc
    25501 gatcacagaa atctttgagc aaatcaaaaa agacggttat gaaaaggtac ttgcggtaac
    25561 aatttccagt ggattgagcg ggacatataa tgtggttcgt ttaattgcac aacagcaaga
    25621 agaattggaa gcatatgtac tagatacgaa aaatattggt attggtgcag gtttttctgc
    25681 gatccaagca gcaaaatggc tggaagaagg agtagaatgg gatcaactga tcagtaatct
    25741 gaatgagtta gtcgaaagaa caaaagtttt ctttaatgtt gcgacattgg aatatcttca
    25801 aaaaggtgga cggatcggtt tagttgcttc tattgtagga accgctttaa agcttaaccc
    25861 aatcatctca tgtaatgaag aagggattta ttacacagta ggaaaagcta ggggacgtaa
    25921 aaaaagtttg gataaaacgg ttgcttacgt caaagaaaaa atcggtaatg caaaggtctt
    25981 caacttagct gttgcccatg gggatgcgaa agaagaagca attgaaatgg aagcgagact
    26041 taaaagagag ttcccacaag caaaccaaat ctactttggc cagatttctc cggcattagt
    26101 agtccacact ggtccgggat tattgggtgt cggtgtacaa gttttagaag gataaatcaa
    26161 aaaaaggaaa cactgaggtg aaaatcagtg ttttcctttt ttattgaagt tagaacaaaa
    26221 aataataaag tattttgcac tttcatttta attttctctt ttgttttata ggcaaagatg
    26281 aaacaaaagc agaaatacgg tataatgaga aggtatgaca ataatatata agaggtggct
    26341 ctttatgaaa aaagttttag ttgtcgatga tgagaagccg atttcggata tcgtcaaatt
    26401 caatttagcc aaagaaggct atgatgttta tactgcatat gatggggaag aagccttgga
    26461 aaaagttgct gaggtagaac ctgatttgat tttacttgat ttgatgctac ctaaaatgga
    26521 cggcttagaa gttgctcgtg aggtacgcaa aacttatgat atgccgatca ttatggtaac
    26581 agccaaagat tctgaaattg ataaagtcct aggcttagag ttaggcgccg atgattacgt
    26641 gaccaagcca ttttccaacc gagaattagt tgcacgtgtc aaagcaaatc ttcgtcgcgg
    26701 tgccacggct gcgaaggaac cagaagaggc agcacctgcg gaattgacga ttggtgattt
    26761 gacgatccat ccagaagctt acatggtgac gaaacgagga gaaacaatcg agttaacaca
    26821 tcgtgaattc gaactactct tttacttggc gaagcacctt ggacaagtga tgacaagaga
    26881 acatttatta caaacggttt ggggttatga ttattttgga gatgtacgaa cagtggatgt
    26941 aactgtacgt cgtttgcgtg aaaagatcga agataatcca agccatccaa attatttagt
    27001 gacacgtcgt ggtgtcggtt actatttgcg taacccagaa caggagtaat tggatgaaaa
    27061 gaaaagtccg cttttttcga tcagtcaatt ttaagatcgc tattaccttt attctgattt
    27121 tattgatctc gatcgaaatt attggtgcct actttatacg tggtttagaa cgttccacga
    27181 tcaatacatt tattaaagat atgaatcaaa cggtggaatc attagcaaca acaatcagcc
    27241 cggaattgaa tcgaaaagat aatgcagatg atgaagaggt aaatgcgaat atcaaaagat
    27301 ttattgaaaa cagtgcgact agcgatatca tcgaaatacg ggtcgttgac gaaaaaggga
    27361 tcatccgagg aacaaccgat gtcaatgaac aaagcgctgt gggaaagaaa aatgattatg
    27421 ttgatatcaa tgatttcacg actaaacgtt atgttgcttt agataacgac gataaacggg
    27481 tgaatatcaa tgttcaaccg atcctgtccc cgaccggcga tgccgttatc ggggctcttt
    27541 acgtcaaaag taatatcgaa caaaaatatt cggaaatcaa taacacagca gtcattttct
    27601 ttactgcctc actaattgcg gcatttattt caatgatcgt atctgtttta gttgctcgtt
    27661 cgattacaca gccgatcggt gaaatgagag aacaagccat tcgaatcgct agaggagact
    27721 acagtcggaa agtcaaagtg catggacaag atgaacttgg acagctggct gataccttca
    27781 atcagttagc tgaacgtatt gaagaagctc aagatacaat ggaagctgaa cgcaatcgtt
    27841 tggatagcgt gctatcccat atgacagacg gcgtcattgc gacagatcgc aggggtaaag
    27901 tcattacgat caatgatatg gctgtttcct tgctagatat caaaaacgag gaagcaatcg
    27961 gacaatcgat tttgacactt ttagatatcg aagaagaata tacgttgcgg aaattattag
    28021 aagatccaaa cgaaatggta cttgaccgtt caaccagtac attggaaagt gatcagatga
    28081 tcttgcgagt agacttttct atgatccgtc gcgaatcagg ttttatcagc ggattagtag
    28141 ctgtcttgca tgatgtgaca gaacaagaaa aaacagaacg ggaacgtcgt gaattcgtct
    28201 ctaatgtttc ccatgaactt cgtacaccgt tgacaagtat gcgtagttat atcgaagcat
    28261 tgagcgaagg tgcgtggaaa gacgaggaag tagcaccaaa ttttttgaag gtaacgctag
    28321 atgaaacaga ccgaatgatt cgaatgatca atgacctctt aaatctatct cgcatggata
    28381 caggtaatac ccagcttcaa ttagaatatg tcaatttcaa cgagttagtg aattttgttc
    28441 ttgaccggtt tgatatgatg gtgggaaatc aggaaaaaaa ttataagatc cgtcgtgaat
    28501 ttacgcaaag agatctttgg gtcgaagtag atacagataa aatcatccaa gtagtcgata
    28561 atatcatgaa taacgcgatc aagtattcac cagatggggg aacgattact tgtcgcttat
    28621 tagaaacgca taataacgtg gttttaagta tcaccgatca aggattgggg attcctaaaa
    28681 aagatctgaa ccgagtgttc gagcggttct atcgagtaga taaagcacgt gcaagagcac
    28741 aaggtgggac aggacttgga ttagctattt ctagagaagt gattaaggcg catcgaggcg
    28801 ctatttgggc agagagcaag gaaggaaaag ggtcgacttt ttatatctcg ctgccatatg
    28861 aaccttatga ggaggaatgg tgggaatgag aatctctgaa aaagtcgttc gtataggact
    28921 cattttgatg gttgcgttaa gcatatactt ctcttatgca atctggctga gcccagcagg
    28981 aaaaagttca atcaacgtag atgagacaaa cactcaaatg atcgagtctc aaagcaatcg
    29041 aaaaacttcc gaagtctttc ttccgcttca tgttacatgg caccattcgg gaatgatcca
    29101 agaaaccaac agtgaaaatc tcgtgtaccg attgcaattg atcatcgata aagcaagatt
    29161 tggaaagctt tctgaagtag tttcaggaga caaatcacag tttgaacagg tgaaaaatct
    29221 gaactctggt tttgagttgg cctataatgc gccttttttg ctaagagaat acaaggaagc
    29281 atttgattta gatttagatg tgtcgaactt ggcaaaagaa agcgaaaata tgtattttac
    29341 tagaatacaa ttcgatcaag gaaataaaaa aatccgattt ttgaattatt cgaaccatct
    29401 cgtatatgaa gcaaatattt cgttgaattg gacagatatc aataaagaat taacagcaaa
    29461 agacacaaaa tggacagaaa tgacaacaga accatcgacg atcgacacgc aatatgctac
    29521 gaaacattcg atcaaactga aaaagtatag ttatatcctg tcacagcaac cctatacact
    29581 ttttagaaat gcttttttcc agaaaccaga tagcgtgaaa aataatgatg aaagcaatga
    29641 attgattttt tacggtggga atgaaacaat gtctatccat tcggaaacgc aagtagttga
    29701 ttttcgtgga gagctgccag agaaaaaaga aaatgctagt gtattttctg aaagtttccc
    29761 ctatattagc aaattaggca gttctttagg gaacctcaga tattttgacc gcagtgacaa
    29821 tacgatcgat tatcggatat tcgtggaagg cttcccggtt tttgggacag acagcagagg
    29881 acagattggt gtggaagttg gcggtgaaac gaatggttct gtgcaagtga atatccaaac
    29941 cagtttgaat acgattcaag tgccgatacc ttcagacgaa gaagtagaac ttgcaagtac
    30001 gaatgaaatc atccagcagt taaaagctaa gggagcagat agcaacaaga ttggttcagt
    30061 gatcgttggc tatacatggc aaaatgtcaa agaaacgaat cgtgtcgttg atctcttacc
    30121 tgaatggtat gtgaaatata aagataactg gtattcagct aacgaccttc tggaaaaatt
    30181 acctggattg gaggcgaatt aattggattt caaaaaaatc gaatggatat ttttcgtcgc
    30241 ttttttaggg ttgaatgtgt ttttattcag cagttatcat gacgcggtat accaagaaaa
    30301 caatgtcatt cgctctaatc agactgaatc aatccaacaa cgtctggcta gcgacgatat
    30361 cacttacacg gggaagattt cgtctgagaa caagcaaggc tattatctta gcgcggagca
    30421 gaccaatctt agcacttcat tagcgaatta tcgcaaggct ggtaaaagcg gcttgctggc
    30481 aagtggtgtg tttttagacg gtgatgtttt gacgaaatct ttatccagtt cagatgctga
    30541 aaagaacgtg atcgatccag ataaaattag tagttccttg gaagattttt tatctaacaa
    30601 agacaatatt ttattcggta atgattatgt gtacacgaaa aacttttctc atttagaaga
    30661 agaaccacta gaaatccaag caagccaaga gtatgaaggc atcccattca atgatgatac
    30721 agcaaaaatc gttctttctt tagaaaaaaa cgataatgat tatagcatca cgaaatacat
    30781 gcagacgcat ttatctgata tcgagcagct gcgcgaaaag acggaactgt atacggaaga
    30841 ggaagcaatc aatacattgt acatcaataa caaaatacca agaggatcaa aaattttatg
    30901 gcgtcagcta gcttattcct gtattctgaa agtccgggaa aaaaatgtgt atgttcctgt
    30961 ttggcatgta gcgatcgaaa caagcgataa agtggtccaa attgagagtg tcaacgcttt
    31021 cagcaatacg atcgtgacaa acaatactgt accaaaagtg gaagatcaat aaaaaagcga
    31081 gtataatgaa aaaagtttac aaagtaagaa gggaagtagg agtcaaatgg ctgaagaaaa
    31141 accagctttt aaaatcagta tcctagcaag tggaagttcg ggtaattcac tttatatcga
    31201 aagtgaaaaa aaacggcttt tggtcgatgc aggacttagc ggtaaaaaaa tcacttcctt
    31261 gatggctcaa attgatcgga caccggacca attagatgga atacttgtga cgcatgaaca
    31321 tcgagatcat atacatggcg tgggtgtctt ggctcgtaag tatcatttgg atgtttttgc
    31381 caatcagcag acttgggaag cgatggaacc actgattgga aatgtaccag tggaacaaaa
    31441 acatctattt gagatgggga aagtcagaac attcggcgat atcgatattg agagtttcgg
    31501 cgtctcccat gacgcagcgg ctccgcaatt ttatcgtttc cataaagaaa atcggtcatt
    31561 cgtcatgttg acggacaccg gatattgcag tgattatatg cgaggtgtca ttgaaaatgc
    31621 cgatggttat ttgatggaaa gtaatcatga tcttgagatg ttacgcatgg gaccttatcc
    31681 gtggaattta aaacagcgta ttttaggaga tagaggacat ctttccaacg aagacggtgc
    31741 acttgtcatg actgatgtga tcggtaatcg gaccaaacga atctatcttg gccatcttag
    31801 caaggaaaac aatatgaaag aacttgctca tttaacaatg gaaaatgttt tgaaagaaaa
    31861 agattttggc gttggacatg attttgaaat ctatgataca gatccagatg aagcaactga
    31921 gctattttcg atttaaacag ttcaatcaat aaaaatataa aaagcagaag ataaaatcaa
    31981 tcagggttgg ttttatcttc tatttttatt tttttagcat attaaaaaaa taaccgtatt
    32041 atcgactgac tacactgatg tgctgctttt catgtttctc atccagtatc tcatctacta
    32101 atgcaatcgc gtaatctgcc atgctgatcg tactgtcgcc agcagcgttt ctttgtaaat
    32161 aatcatcact taacaaataa gtacctgtac gtttgccatg tggatcaaat aaagaagctg
    32221 gactgatata agtccaatgt agatttttag attgtttcaa ttttaacaat ccttcgtaca
    32281 tattgaaagc tgttggatag aagggtgcat ttttaggtac ttggtcaatc atacgagtcg
    32341 tcttttctgt atctaagaat aacgaagagg cacccccaac gacaattaag cgtgtatcgg
    32401 ttccttcaag catctcaatc aaatgggaca aagaagtttg atgcatttct tcttttcctt
    32461 tgggagcgtt gaaagcatca acgacaacat caaaagatgc aagatcagta gttgtcaact
    32521 caaataaatc cttttgtata aacggaacat ctgatggcaa tttttctgga tgacgcacga
    32581 ttgccgtcac gtctaaatct ctggacaatg cttcgtctaa aataaatgaa cctgcatggc
    32641 cagtagctcc aatgagtgca attttcataa aaaacctcct tttttattcc tgtccattgt
    32701 aagcagttgc tgaatattcg tcaaagaatc cgatttttga aaagctcagt gcgcattcct
    32761 ttcatggttc gtgatcattg ggtatagtta aattaagaaa actgattgat aaaaatagaa
    32821 gttgaaggag ataagaaatg aaagagtggt tagatcgtgc ctttttacca atgataacat
    32881 ctgttgtgaa tcttagaccc ttaactgctt tgcgtaatgg aatgacttac acgatcccaa
    32941 taattattgt tggctcgctg tttttcttat ttgctcattt tcctgtggat tacatcgctc
    33001 agtggataga caaaacagga ttttctatgt attttacaca agtataccag gctacatttg
    33061 atgtgatagc cttttggatc gtttttgcca ttgcatacgc ttatgcaaaa gaagaagaca
    33121 tcgatcgctt accagtcggc ataacagcct tgtctgctta tctactgctt attcagccac
    33181 taaatactca gatagatact gtctggacag gtgctgaagg gatactagga gcagttacaa
    33241 ctgggctcat catcggatgg ggctatgcac gtttagtaag acttgcgaaa gggtggaagc
    33301 aaagagagaa gatacctgaa aatgtttggc agtcttttac ttcattgatt ccaatcattg
    33361 ttatagtcac ttttacgctt atcggttcag cggtttttca acagttgttc agaatgactg
    33421 ttgttcaagg agtatggaca tatgtccagc ttcccattca atcgttcata gacacgttgt
    33481 ttggtgtgct tttattaggt ttttttgttc cgtttttatg gttatttggt cttcatggat
    33541 ctgcgatggt caatgggttg gttagtcccg tcttgcaagc gaattcatta gcgaatgcag
    33601 aaatcttagc ctcaggacaa gagttgacgg tggcaaatgg cggacatatc gttactcagc
    33661 aatttctcga tcagttcatg acggttacgg gtgctggatt gacgttaggg gctgtatttt
    33721 ttatgacggt ttttgcgaaa tcgaagaaat acagagaatt aggaaatctt gctcttttac
    33781 cagccttttt caacatcaat gaatcaatta ttttttccac accgatcgtg atgaacccga
    33841 tgatggctgt tccttttatt ttcgcaccgg tcgtttcagg attgattacc tattccgctt
    33901 tatattttgg attcgttcct ttatttacag cagtccaagt cccttggaca acaccgccga
    33961 tcctctcggg tttcttagta ggcggagtgc ctgcagcgat tttacaaggt atcgtgttgt
    34021 caattagttt tttcatttat ttcccatttt tgaaaaaaat agattctgca aactgcaaaa
    34081 aggaaagaca gacaaaaaat gatggagcag cagaaaagat gaacgactaa aaaacaaaaa
    34141 tactcctgtc gctttagatt gggaaaatca ccaatctagg atgacaggag tatttatagt
    34201 catgtttata ctagcccttt ttggacagct aattgaacaa gaacaactaa tgtgttcatt
    34261 cctacgtgtg tgatcatcga tgtccagatc cgaccagttg ctttgtactc taggctgaag
    34321 aagaaaccta ggaaaaagta gattagcaga tgaccatcat tatgagcaaa cgcaaacagc
    34381 aaagagctga tcactgctcc gacccaaaaa tttgaatatc gactgatgat ccccagtata
    34441 ctgcggcgga atacgaactc ttccatgatg ggtccagcaa cagttgtcgc caaaataaaa
    34501 gcgggtgctt ccatgatcat ctggatgata ttttgtgtat tctcggaagg ggttgctttg
    34561 ccaaacaata cagattctaa catgacagcg atgctttgca gaatcaatgc gacaaagatt
    34621 ccaaccaatc cataaataac aatctttgga gcagaagctt tttttaccgc tgattcgatc
    34681 gctaagggtt cacgttgctt aaaataaaaa atagctagga caacggcgcc tagtatatac
    34741 atgactgcag ttgctgtgat caattcagac gtagatttta caagtcctat cgaagctagg
    34801 atcaagggtg agataaaaat tagaccatag caaaaaatgc tgaggaagct atattttttt
    34861 aaagacatgt aattcctcct tttaagggat aacaagcatg caaggcaagc ttatatcgat
    34921 tgatttcaat tatatcataa tttcaataag cagctgtcag aataaaattt cttttctttg
    34981 atttaaaagc ttgcaaaaaa gagggagaat gagtattatt aaaaatgtag gttagcactc
    35041 gaaggttaag agtgctaaca gattaaaaaa agagttatcg gagggattta tcgtgttaaa
    35101 accattaggt gatcgcgtca ttatcgaagt cgcaaaagaa gaagaaaaaa ctgtcggagg
    35161 aatcgtttta gcttctgctg ctaaagagaa accccaaact gggacagttg ttgcagtcgg
    35221 tgaaggccgt ttgttagaaa acggtgaaaa agttcctgcc gctgtaaaag caggcgacca
    35281 agttatgttc gaaaaatacg ccggtacaga agtaaaatac gaaggaaaag aatatctgat
    35341 cgtcgcagga aaagatatca tggcgattgt tgagtaaatt tggaagataa aaaaataatc
    35401 aaaaataatc aatgaggtga atcaatcatg gcaaaagaat tgaaatttgc agaagacgca
    35461 cgcgcagcta tgttacgcgg ggtagataaa ttagcagata cagtgaaagt aacattagga
    35521 cctaaaggcc gaaatgtcgt actagaaaaa tcttacggct ctccattgat cacaaatgat
    35581 ggtgtgacga tcgcaaaaga aatcgaatta gaagatcatt ttgaaaacat gggtgcaaaa
    35641 cttgtatcag aagtagcatc aaaaacaaat gatattgcag gagacggtac aacgacagca
    35701 actgttctga cacaagcaat cgttcgcgaa ggtttaaaaa acgtaactgc aggtgctaat
    35761 cctctaggca ttcgtcgcgg gatcgaattg gcaacaaaag cagcagtcga agaattgcat
    35821 aatatctcaa ctgttgttga ttcaaaagaa gccattgcac aagtagcagc tgtttcttcg
    35881 ggttctgata aagttggaca tttgattgct gatgcaatgg aaaaagttgg aaacgacggt
    35941 gtcatcacaa tcgaagaatc aaaagggatc gaaacagaac tagatgttgt agaaggtatg
    36001 cagtttgatc gtggttactt atcacaatat atggtaacag ataacgacaa aatggaagct
    36061 gttctagaaa acccatatat cttgatcaca gacaaaaaaa tctctaacat ccaagatatt
    36121 ctgccattgt tagaacaaat cttgcaacaa tcacgtccat tgttgattat tgctgacgat
    36181 gtagatggtg aagcattgcc aactcttgta ttgaacaaaa tccgtggaac attcaacgta
    36241 gtagctgtaa aagctcctgg tttcggcgat cgtcgtaaag caatgcttga agatatcgct
    36301 attttgacag gtggtacagt aattactgac gatctaggtt tagaattgaa agatgtaaca
    36361 atcgaaaact taggaaatgc ttctaaagtt gttgtagata aagacaatac aacgatcgta
    36421 gaaggttctg gagaaaaaga agcaatcgaa gctcgtgtgc aattgatcaa aaaccaaatt
    36481 gctgaaacaa cttctgattt tgaccgcgaa aaattacaag aacgcttagc taaattagca
    36541 ggtggtgtag ctgttgttaa agttggtgca gctactgaaa cagaattgaa agaattaaaa
    36601 ttacgcattg aagatgcatt gaatgctaca cgtgccgcag tagaagaagg aatggtctca
    36661 ggtggtggta cagctctagt gaacgttatc agcaaagttt ctgctgtaga agcagaagga
    36721 gacgtggcaa caggtatcaa gatcgttgtt cgtgctttag aagaaccaat tcgtcaaatc
    36781 gctgaaaacg ctggttatga aggatcagtg attgttgata aattgaagaa cgtggaacta
    36841 ggcactggtt tcaatgctgc tactggagaa tgggtaaaca tggttgaagc aggtatcgta
    36901 gacccaacta aagtaacacg ttctgccttg caaaatgccg cttccgtttc tgccttgtta
    36961 ttaacgacag aagcagtcgt tgcagataaa ccagaaccag cagcgccagc agctcctgca
    37021 atggatccat caatgatggg cggtatgatg taagaaaaag caacttgctt atctacaact
    37081 gatcattagt gaaagacaga taaaaagagg ctggaacaga agcgtttaac tccaagaaat
    37141 aagaagaaat tcacggaaat tgcttttcaa atttttgtga atttcagctt atttccgaag
    37201 gagttgcttc tgcttccgct gtttatacgt gtttagagcg tgagacaaaa gtgttcttta
    37261 cttttgtccc acgctctttt ttgtgtgtaa ttataatgaa aatagttatt tttaatagtt
    37321 ataatatttt atctattaaa agtaaatatc cattcacgat tacgtccata taattgtgta
    37381 aaagtgaagc atcaaaaaag ccattgatct cagttaggag tacaatgttt tttgcagaaa
    37441 acatactcga ataggactga tatcaatgac tcaagtacat tttacttttg aaagcgaaga
    37501 aattcaagca attataaacg aaagcggtgc gaatgatacc gcaaaaacac ttatgactat
    37561 catgtttaat cagctgatgg aagaacaacg aaatcaatat attcaggcaa atgcctatga
    37621 acgttcagaa gaacggcaat cacaaagaaa tggttactac gatcgttcgt ttacaactcg
    37681 aattggtacg ttagaacttc atgttccacg aaccagagac ggtaagttta gtcctactat
    37741 ctttgaaaga tatcagcgaa gcgaaaaagc tttgatcgct gccatgattg aaatggtcat
    37801 ttctggtgtc tccactcgca aagtaactaa aacagttgaa ctgctaacgg atggtgcgac
    37861 tgtttctaaa tcattcgttt caaatctgat gaaacagttg gatccatttg tttttgagtg
    37921 gagaaaccgg agcctagaag gctcggaata tccatttttc atgtgtgatg ccctgtatat
    37981 gaaagtaagg gaaaatcatc gtattgtctc aaaaggtgta tatattggta tcggcattga
    38041 ttctgatgga cgacgtacga ttcttggttt tgatgttcaa gacggcgaat cggaagataa
    38101 ttgggatacc gtttttcaat cgttcgttca acgaggtttg ttcggtgtaa aactggtcat
    38161 ttctgatgca cacaaagggt tagttaaggc tgttcgcaag aactttttag gtgcgagctg
    38221 gcaaagatgc caagcccact ttttgagaaa tatttttgat aagctgccaa agaaagtttc
    38281 ttctgatgta aaagacgagt tgaagagtat ctttaaagcc tctgaacttg aattgacacg
    38341 tgaaagaaaa gagcacttct tggaaaaata tggttgtgat tcaaaattaa gtgctgcttg
    38401 cgacatttta gaaaacggct ttgaagatgc tattcaaatt ttgtcttttc ctgaaaatat
    38461 ccgccgaagg attcgtacaa ccaatgtgtt ggaacggttg aacgaagaga ttcgccggag
    38521 agaacgagtg attcggattt ttccaaatat caattccatc actcggataa ttggaacact
    38581 tttgatggaa aaagacactg agtggctggc ttctccacga aaatatttag aatttaattc
    38641 gaataacatt tagcaccatg aacctgtgcg agtctcttct ttctgaggag agacatcaag
    38701 agtcgctgcg ctactgctcc tgacctctct cctcagaaag aaaggcatgg cagttaggtt
    38761 cacaacgcaa aacattgtgc ttttgatgag gttgaacttt tacacaagat tgtggacttg
    38821 actcggaaat accatcaatt taaaagagat atccagatat aaactaccat ttctctttac
    38881 aggcaattta atgttgaaga tccgcttcgt ttttacatca aactttgata taaagcaatg
    38941 atttcttcta ctgtcgtttc tcttggattt cctggtgtgc atacatctcg caaagcatct
    39001 tctgcaattg ctggaatatc tgcttctttt acacctaatt cagaaatggt ttctgggatt
    39061 ccaaccgctt tgctcaaccg atcgatctca ccacaagccg cttctcttac atcttctaaa
    39121 gacatttcag ctgctccttt aatccctaga acaagagcga tctcacggta cttttctcca
    39181 gtatattctt tgttatattt catgacagtt ggcaaaagtg ctgcacacgc tacgccatga
    39241 ggaatgttgt accatgcact taacgggtga gccatcccgt gtaccagtcc taaaccgaca
    39301 tttgagaagc ccattcccgc aatatactgt cctaatgcca ttttttctcg cccattttga
    39361 tcgccatcta cagaagcttc caaagaatgg ccgatgattt cgattgcttt taaatgcagc
    39421 atatcagtca tttcccaagc gccttttgtg ataaaccctt caatagcatg cgtcatagca
    39481 tccatacctg ttgatgcagc caattttttc ggcattccca tcatcatatc gctgtcgatg
    39541 aacgcgacga ttgggatatc atgaggatca acacaaacaa attttcggtg atttacttca
    39601 tctgtgatga cgtagttgat cgttacttct gctgctgttc cagaagtcgt tggtatagct
    39661 aatataggta aacatggatt tttcgtatca gcaacacctt ctaaactaat gacgtctgaa
    39721 aactctggat tcgtcaaaat aatcccaatg gcttttgccg tatcgattgg cgaacctcca
    39781 ccgattgcga tcaaacagtc tgcctctgcc tctttaacaa aagccactcc atcttttaca
    39841 ttttggatcg ttggattagg tacgatcccg tcgtataaag cataagcgat tgctgcttta
    39901 tctagaagtt ctgttacttt tgttgctata ccgtgctcga tcaatccttt gtcagtaatg
    39961 acagctgctt tgccgaatcc tcgctgctga aattcaggga cgatcgactc aatcgctcct
    40021 tttccaaaat acgatgtttc atttaaaatc attcgattac tcattttcta ttcctcctaa
    40081 tttgtattat ttttcacaat aaaaacgaaa gaaccccaag caaccggttg cccagttgct
    40141 tgttgctaca gttcaaagac tggcatcaat tcccaactga ctggagaggc atctggattt
    40201 gtctccatga tatcttccat gtacttccac catttttgat tgatagatgt atttgccatt
    40261 ttttcccata acttttcatc ttcgatcact aagtaaccaa ataacaaact tgtactcgga
    40321 tcaagataga tcgaatagtc gatgaccccg tgttcacgaa gtgctgtacg catttctggc
    40381 caaagttcat catgtctttt tttgtattct tcttgttttt ctgggtaaac tttcatgcaa
    40441 aaagcttttt tcaccatgtc agacacctgc tttcgtatga acttcctcca aataacctgc
    40501 ttttggagtg acaccaaacg catctgctaa atgccacagg ttttcgtccg tgattgtttg
    40561 caagattggt ccttgcgctt tgacgtacgt atagacttct gctgcctttt ctgctgtctc
    40621 gatcaaacca aagacttcat ccatgtcttt tcctgacccg tagattccat gttgtggcca
    40681 taaaaccaat cgagtttctt tcattttttc agctgttgct tccccaatct catttgttcc
    40741 cggaaccatc cacggaatga tggaaattcc ttctggaaaa acaactaagc attccgtaca
    40801 catttgccat agcgtacgac taaaggaacg ttcgtctaat tcatgcgtaa aactcatcgc
    40861 taatagatga ctagcatggc aatgcataac gatccgatta tctgggtcta cttctaaacg
    40921 ggcgatatga ctcatgaaat gacttggcaa ctcgcttgta ggaaggcatt cgttttccag
    40981 cccccacaaa agttccagtg ttttgccatc ttcagcaact cgaacgattc caagattctc
    41041 ggcaggttcg ctcgctacat ttttaaaata tcttcctgaa ccagttacaa taaaatagca
    41101 tcctgctaaa cggctggcat caaaaatcat cgagatgcgg cggatcactt tttcaggatc
    41161 caagaacggt gtgatctctt cttcttttag taaataagaa atattcccgc catttctttc
    41221 atcccatccc aagcgataaa gattcgacgt cgtttcgatc atttctttca cataaggcgc
    41281 ttgtaaaata tctatttttt tcaatatagc cacactccta ttttcttaat tccaacacgt
    41341 ctttttgata ttcctggact tcttccagcc aatccaaacc aactggaact tgatttttgt
    41401 aacagaaata attccataca tcggcatacg ggaaatcttt caattcttcc gtataagcca
    41461 ttcttgaagt aaagtcaaaa gccagctcca tttctttcaa tcgctcaact ggttccagca
    41521 tagctttcag cagtgctttt tgtgtatttc tcgtaccgat gacccaagca gcgatacggt
    41581 tgatcgttgc atcaaagaaa tccagaccga tatttgtttt ttccaacaat tcgttacgga
    41641 ctaattcttt agcgatttcc tgcaattcat catccatcaa aaccacatgg tctgaatccc
    41701 aacgcactgg acgggaaaca tgcaacatga ttcctttgct gaataaagct aatgaagata
    41761 atttattgga aatcacttct gttggatgaa aatgtccagc atctagacaa acaagtttgt
    41821 cacgagtcaa cccgtaaccc atataaaatt catgggaacc gactgtataa gcttcagcgc
    41881 ctaaaccaaa tagtttgctt tctactgcat cttgtacata ccgttcatct aactcttctg
    41941 taaaaatttc atcgagtgat tccattaaac gttcccgtgg ggtaaaacga tcaatcggat
    42001 tatctttcat cccatcaggt acccaaaagt tattgatact tgtttgccca gtttctttcc
    42061 caaagtactc cgcgattttg cggcttcttt tcccgtgttc gatccagtat tcacgaactt
    42121 ctttatctgg atgagccaat gtaaagccgt ctttcatcat tggatgcgaa aagaaagtcg
    42181 gattgaagtc aagaccgatt tcttcttttt ttgcccactc tacccatttt tcaaaatgtt
    42241 tcggctcaat ttcatttaga tcgacagatt cttctgtatc caaatagata gcatgaaggt
    42301 tcaatttgtg tttgcccggt atcaacgaat aagccttctc cagatcttgt ctaagctctt
    42361 caggtgttcg tgcggctcct ggaaaattcc ctgtcgccat gattccaccg gtcaattcgc
    42421 catccggtgt caagaaacct ttgacatcat ctccttgcca gcaatgcata gaaattttga
    42481 tgtcctgtat cttttctagt acagcatctg tatctacacc gatagttgca tatttttctt
    42541 ttgcttgttc gtaatttctt tcaatcgtgt tcatacgatt actcctttga ttttattcac
    42601 gtaccattcc atcgtgtttt gcttattttc atctgggtaa aaagtttcac atggaaacga
    42661 ctgtttgatt acatttcttg cttgttctat attcgaaaac tgacctgtac tgagcatttg
    42721 catggcaaca ttcccgattg ctgtggcttc actaggtcct gcttctacaa tgattccggc
    42781 ataatctgct gtcaattgat tcagtagacg attattcgat ccgcccccta cgatgtgcag
    42841 cttttccaat ttcgttccag ttaagtgttc tactttgatc agctcttgtg cgtaacaaag
    42901 agcaagatta tcatacacac aacgagcgat agctcccgat gagacaggga ctggctgctc
    42961 tgtttcaaaa cagtattgct gaatctcttc tatcatattt gggggattca aaaatcgagg
    43021 gtcattgatt tccatcactt gctgcagcgg tttttctttt gcagcaagct ctgccaattc
    43081 cgcataactg tactcgtact cttgcattcg ggcaacttcc tggatcaacc acatccccat
    43141 gatgtttttc aaaaaacgca ccgtgttatg ggcaccccat tcattcgtat aattttcaga
    43201 aaaagcattg cgagaaacat tggcgatctt tgtttcaatc cctaaaagcg accatgtccc
    43261 actgctgata taaccccaac gttctccttg acctggcgtt cctaatacag ctgacgccgt
    43321 atcatgactt gctactgtga taaaggtcac atctggcaaa tcatattttg aaaattgttc
    43381 tttttgcaga tctcctagta tcgttcccgg ctctgtcaat ggtggaaata attccttcga
    43441 gacaccgatt ttttcaagaa gttctatttc ccattctttt gtccctgcat tcagcagctg
    43501 catagtagat gcattagttt tctccgtcac catgttacca gtaaagacat accctaaata
    43561 atcaggtatc agtaacaatt tatgtgcttt tgccaaattt tctttttctt cagcaaacaa
    43621 ttggaacaag gtattgaaag gctggatctg aatccctgtc tgctcataca acttatctag
    43681 agaatagatt tcagaaaact gttctactgc tccattggta cgcttgtccc gataagcaat
    43741 cggatcattt atccgatccc cttgttcatc aatcagacag taatcgactg cccatgtatc
    43801 gatccccgca aaaaggcgat caatccctgt ctgctttaat ttttcaaatc caatcaaaat
    43861 ctctttgata agataatcga tcttccagcg atcgtgaccg tctttgttcc gaaagccgtt
    43921 tttgaaccgg tgtacttctt ccaagatcaa agaacctttt gcatccctcg tacttttcat
    43981 tagtcgtccg cttgatgcac cgatatcgat ggcaacaaat gttggattca tttctccacc
    44041 tcttttactt tattgttgat tagcacttta ttaataaagc gttatcattt attgacaatg
    44101 ctatcataat acagaatctt atattttgct tcacactatt ttggtatata attgcattat
    44161 cttgacaagt tgggagtgtt tacatggatt actgggaaaa agccaaccac tcatggtcgg
    44221 aagactcaaa aagagtgatc ttgacgccta cacaaagaag caggaattta tttttttata
    44281 tccaagaaat cggttacttc aaagcaccaa tcccttacta tactgaacga gcacatttac
    44341 cttcttactt gataaagttt actttgtctg gcaaaggttc gcttacatat aatgataatc
    44401 agcaacagct aaaagctgga gacttatttt ttattgactg ccaagaatat cagcgttatc
    44461 aaacgatcag cgaagaacca tgggaaatgg attggatcca tttgaatggt cagaacgtcc
    44521 aatcatttta tcaggagttc acaaaagacg gccataacac cttccattca acaggagatc
    44581 catcagaaaa caagatccac catttgatca cagaattgat ccacatgcaa caaggcacga
    44641 atgcccgtac cgatttccag tcctctgtag ctatccacga gttgctgaat gaactgattt
    44701 tgcaaaagta tcagctagat ttcgaagaag aggatattcc cgcctacgtt tacaaactga
    44761 agcaatactt agacgaacat ttcaaagaaa cgattacact tgatcaatta gaacaacaat
    44821 tcttattgaa taaatatcag ctgagcaaag atttttccaa atttatcggt tctccaccaa
    44881 tcgattattt gatcagcaaa aaactttctt acgctaaaga ccttcttcgg tatacagaat
    44941 ttacggttcg aatgatttcc gaagaaatag ggattgagaa ttttgcttac tttagccgcc
    45001 tgttcaaagc aaaaacagga ctatcgccga gagactttcg taaattcagc tgattcatat
    45061 aatatgtacg tcattttttc aaaatatttt gaaatatata gataaaaata aaataaccgc
    45121 agtttctgct tctatgtaaa caacagcagc tactgcggtt attttttagt gatcattctc
    45181 attgttcatc ttcaaaatgt tcataatgaa ccaactgttt ctgtgtatca cgtttcaaaa
    45241 attctttagg cgttatgccg aactctctct tgaatgcttt cagaaaatgc ggataagaag
    45301 aaaaaccact tttactatat acagcactag agctgcggta ttctcgcaac aagtgcttgg
    45361 catagatcag ctttttcttc attacatact gatgaaacgt aaatccagtg tactttttaa
    45421 attctcgtgt gatatggtat ttgctgacaa aaaactgctt ttccatttcc tctaaagaaa
    45481 gcggatgaga taaatttttt gagatatatt gtaacatttg ttccacacgc tcattaggaa
    45541 ctacagcatt ctgttcttgc gcactctctt ctttttgtac aacttgattg agaaaaatca
    45601 agtattctac cagagcttgt tcatatttta tgtcttcacc gtaaattttt ttatcaggag
    45661 gatggtcgat aaatgataat ttttgcttca atacttcctg ttctatttgc aatacacgac
    45721 tcctcttctc tcccattggt tgaaaacaag ccgatagatt agaccatggc gtggaacgac
    45781 tttctagaaa agaaggggtg acaaagatat agacacgctc gaatacatct gaagattggc
    45841 ggacgatacg atgcaaatca ttggaatgga tgagcagcac acttcctgct ttgatcgtaa
    45901 attgtcgtcc gttcaaataa aaagtcgctt ctccctttaa tgtagcatgg atctcataga
    45961 aatcatgatg atgatagatc gttttatttt ggctgacgac atctttgaca tgatagattt
    46021 catactgatc agaaaccatc gtattgactt gttcaccgct gattttcttt tggagcatgt
    46081 gccgcacctc cttttgttca cttatgcaca attttattat accacgtttt ttgtaatcaa
    46141 aaagagctgc ttttatatac tcttataaaa aagaagagac gtggcatctt ctagccccgc
    46201 cccctccatt ctaaatatta gttaaccaac gtcttcttta cattattcaa gaagacttct
    46261 tccataacga atcccatcat cttttgtttc ttcaagaacc acatacaggt acggacgatt
    46321 cttcatgatg atttctttga tttttgtgta ctcgatcaca ccgtctccca aatttaccgg
    46381 cacgattttt tcctgttcga cacgaaaatc tttcaaatga accgcacata tcttttcacc
    46441 aaaacgttca aaagcttctt ctaccaattg gacttgttct tgataatcac tacttgtgat
    46501 caaattggtc ggatctaaaa tgattcctag atagtccgaa tctactgctt ggatcagctg
    46561 ctctaccctt gctaaagaat acaccggatg attcaaacct ggttctatcc ccaccatcat
    46621 ttggtatttt tctccagctt ttaccaagcg acggataacg gacacagctt cagcaaatgc
    46681 ttcatctgtg aaattttctt ccgtatattg gatttccggc aaaacacatc cagtttccga
    46741 agcaaccatc gacgcaccaa aggaagaagc atagcgaaga tactgctcga actttgtcag
    46801 caactcttgt cgtatcgtta aatctggatg aatcatattg atatagcaac ttaaaattcc
    46861 tacggtcact tcttgctttt ctaaaactcg tttgacataa tttcccatcc cagaattgat
    46921 ctcatttgcg ccagaaggta tttcaggaaa cgaaagacct aaggcgagct gcagtgtatg
    46981 aatcccttgt tcttttgttt tttctgcaag atcttctatc gtttggactt cggaaagatc
    47041 atgtcctcta attgtaatgt tcaacataac tattctccta actcagcata ttgggtagcc
    47101 acatgacgat ttgcgggaag taagtaatca aaaacaagac gactaagata accccataaa
    47161 aaggaagaag cggtttcagc attgcctctg cttttacgcc tcctacactt gcaccgacat
    47221 atagtcctgt acctactggc ggcgtgatgg agcctacaca cagattcatg attgaaaaca
    47281 acccgtaatg tacaggatca acccctacac ttgtgacgat tggtaaaaat attggtgtaa
    47341 aaatcaagat agcaggcgcc acatccataa acatacccac cagtaataac acaagattga
    47401 ctaataacag aataataaat ttattatccg ttacgcccat aatcaacgaa ctaattgcct
    47461 gaggaatacc ggtaaacgcc atggcgaaag acatcatgga agaagtagcc aataagaaca
    47521 tgatcgtccc tgacattgct actgattgaa acaacatatt aggtatatct tttagtttta
    47581 ccgttttata gtaaaacata gcaatcaaca gggagtaaat gacagctatc gctgaagctt
    47641 ctactgctgt aaatatcccc gtcaagatac caccgataat gatgacaatc aatagaaggc
    47701 taggaatcgc ctcttttatt atttttccag catgagccat ctgtcctttt tcgacaacag
    47761 ggtatttatt ctttttagca acgatgaatg ctactagcat gattcctagc gcccacaaag
    47821 cgcctacgag ataacctccc ataaacaaag cagagatcga agcaccacca gcaacaagtg
    47881 aatacatgat aaaagcagta cttggaggaa tgaccatacc tgtcggagct gacgcgatat
    47941 tgaccgctgt cgcaaacttc cggtcatatc cttcttccac ttgctgaggg atcaacactc
    48001 cgccaatcgc ggtagaagcg gcaatggccg agctagagat ggaaccaaat aaagcattcc
    48061 ctaacacatt cgtatgagcc aaagcccccg gcacgcgccc gacgaacaac atcgcaaaat
    48121 ccactagttt cttagctatc cctccattat tcatgataat gcccgagaga acgaaaaacg
    48181 gaatagcgat cagcgaaaaa ctattgatac ttcccaccat tttttgagct gaactaaaag
    48241 cagaaacatc aaacggtata actaacaata aagtcatcat cgatgagaat gcaatactta
    48301 tagcaatcgg catccctaat acaagcaaca caacaaatac aaaaaataac actaatccgg
    48361 cttctattgc cattttattc ctccattttt atattttttc ttgaattgct ggcttgttgc
    48421 ttaatgtatc gattagattg atcaggctat agataatgat gagtaccccc gatacaggta
    48481 ctgacagata aacatatccc ataggaatac ttaaagaagg cgagatctgc cccattgtca
    48541 atgtgacagc attccatccg ccatacacca tgatcatcgc agcaaatacc agaaataaac
    48601 tttgaacaac aacttctaaa attttacttt ttttgccagt taatttttca cttaaaaccg
    48661 ttacggctaa atgtgctttt tgtcccacaa catacgcgga agacaacatg gcaaaccaga
    48721 caagaccgaa tcgaacaagc tcttccgtga ccgtatttgg atttcctaaa accgttctgg
    48781 aaaatacttg ccataaaaca acagcgacca tcccaacaag caaaacagct cccaggatct
    48841 ctaatacctt gtttaaaatc attttcatca cagctacgct ccttgctctt ggatcttttg
    48901 ataaaggtct ttgtaggtag gatcgttttt gaatatatca tgaagtgggg caactgcctc
    48961 tcgaaacggt gtattatcta catcattgaa ttggactcca tgttcttctt gtgctaaagc
    49021 tttttcttct tcaattgctt ttgcaaaagc ttcaatttca aaaaccgttg attctttcgc
    49081 tgcttcttca atggcttttt cctgctcatc agttagctgt tctttgatgg catcattcat
    49141 gatgatgata tctggcacac gtgtatgctc atcataggaa tagtatttag ccacaccacc
    49201 atgtccagct gtatacaaga caaattcatt gttttctgat ccgttgatca aattggattg
    49261 aagagacgtg tacacttcat cacttcccat aggtacgggc gaaccgccta aaagctctac
    49321 cattttgata gcggtttcgc tttgcatcac acggatcttc tttcccttca aatcttccgg
    49381 cgtatggatc ggaccgtcta ccatataaaa gctgcgctga ccagaatcat aataggtcaa
    49441 tccggtaaat cctaaatttt tggtcgattg gtagattggc tccattattt cttcattatc
    49501 catcacctta aagaaatgtt cttcatcatt gaatagataa ggaactgaaa aaatcgaata
    49561 atctttagag aaactttcca acgctgaggc acttactttt gtgaaatcta ctgctcctga
    49621 ctgtgtcaat tcgatcagtt cacgttctcc acccagttgt ccatctggga aatactggat
    49681 ctgtacggaa ccatctgttt tttcttttac taaacgccca aattcattca ttgcatcaat
    49741 tactggctgg ctgttgcttg cataagcaaa gcgcaaggta actatctcat ttacatctgc
    49801 atctgcgaca gttgccacac tagaattatt tccgcagccg cttagaccta gcagacctag
    49861 gctaagcgaa cttgctgcaa acagcagtga tgtttttttc atatgggttt cctttctttt
    49921 aacaataaga gacgagcaat tctgtcaaac aaagcaccgt taacgactgt ccataaggca
    49981 ttgctgtgat tccgatattc cgataaaaat ctaaactgtc ccccattcct gtcccgattg
    50041 aaacgttttg aacttctcct ttttcatcga tttgttcaag caatccttgg atagcacgat
    50101 aagcgacttc tttatattct tgtggtaaat aacgtttatg gaccgctttc aaaataccgt
    50161 aagcaaatcc tgcagttgca gaagattcta cgtaagaaga aggatcatcc aacaacgtat
    50221 gccataaacc gctttcagac tgataagttt ttaatgcacg cacttgtgct tcaagtgttt
    50281 cgatcaataa tttgcgcaat gcatcttctt tcgataattc taagatttca atgatctctg
    50341 gaatagcaat cgtcaaccag cagtttccac gagcccaaaa tgcttcggca tagttatgat
    50401 ttccttcaaa cgtccagccg tgataccaca atcctgttcg tttatccatc aagtatttga
    50461 tatggatcat aaactggtaa cgagcttctt ctaaatattc tgggcgattc aacagtttcc
    50521 cgatttttgc caaaggaagc actgtcatca tcaatgtatc gtcccacaac tgattcttat
    50581 tttccggtcc atatgtggca tgctgtaacc cgtcttcatt tgtccgaggc atgtcattca
    50641 tgacccattc tgcccattgt tctaaataag gcaaatagcg tgaatctttc gtgtcttcat
    50701 acaagtaagc catagtcaat agcggtgcca ttgtattgac gtttttgggc ggagcgcctt
    50761 cttccatacg atcctcaaac cattcattga ccacttgata agcgcgagga ttttttgtca
    50821 tctggtaatt tttataaata ccatatagac cgacaccttg tggccaattc cacactgtcc
    50881 agcttttgtc atctactttc agtccatcaa agtccaacaa aaattcacca ttatcgtctt
    50941 tgatcgttgt caggttttca acgagcaggt caattttttc tttcactaat tctttaggta
    51001 caaaaatctc ttctttaata atcatcttta cgccaccttt ttaaacgctt tcattttata
    51061 tccttaatat agcagtaata ccgtccacta taaatatcgc tttttgaaca aaaactgtct
    51121 aaaattgacc taaatgttaa ataacaaaag taactttcag ttacttggaa attaaaaaaa
    51181 gaacttggag aaacaacgtt ttccaagttc tttttcatct attttcttat cttttttcta
    51241 cgctttcttt tgcttatccg ttgtaagcat caatgatgat ctgtttcaat tcagagatca
    51301 atggttcttt cggattagct gtcgtacatt ggtcttcgta agctaattca gccatacggt
    51361 ctacggttgt atctagtgtt tcttgtgtga ctccttgagc ttttagactc atgttgatgc
    51421 cgacagattt tcctaattca taaacagccg ttgctaaagc ttcgactaat tcctctgttg
    51481 tttcgccttt cagacctaag aattttgcga tatctgcgta atctgtatct gcacggaaat
    51541 agtcatattt agggaacatc gcatgttttt gtggatcttt tgcattatag cggataatat
    51601 gcggtaaaag aatcgcattt gtccgtccat gagggatccc gtattccccg ccgattttat
    51661 gtgcgactga gtgacaaata cctaggaatg cgttagcaaa cgccatacca gccattgtag
    51721 aagcgttgtg cattttttca cgtgattcca tatcaggtgt ttttactgat ttttctaaat
    51781 agtcaaagac taacttgatt gcttgtaggc tcaatccacg tgtataatct gaagccatga
    51841 ctgaaacata ggattcgatt gcatgtgtta acacatccat cccggtatct gctgtgacag
    51901 atgctggaac tgacatgacg aattgaggat cgacgattgc gacatctggc gtcaatgcat
    51961 aatctgctaa cggatatttc acatgtgttt cactgtcagt gatgaccgca aatggcgtta
    52021 cttcagaacc agtccctgat gttgtcggga tacatacgaa ttgtgttttt tctggtttgt
    52081 cgatcttata tgtccgttta cggatatcca agaatttttg ttttgcacca aagaaagatg
    52141 tgtctgggtg ttcatagaac atccacattc ccttagctgc gtccattgca gaaccgcctc
    52201 caagagcgat tactgtatct ggcttgaagt ctgccatcag tttcgttcct gcgtatacgg
    52261 tatttgtcga tgggttcggt tcaacatctg agaagacttc gatcttcaca tcatttttac
    52321 gtcgtgccaa tacttcacga actgtatcgc agtaaccgaa ttggaccata ccagggtcac
    52381 aaacgatcat gacacgttca acgttttcca ttttctctaa gtacaataga gagttctttt
    52441 caaagaagat ttttggcggt aatttgaacc attgcatgtt atttctccgt ttcgctacag
    52501 ttttgacatt gattaagttt acagcagaga cgttacgaga aacagagttc ttaccataag
    52561 aaccgcatcc caaagtcaat gacggaatca tttcgttata gatatcaccg atgccgcctt
    52621 ctgcagatgg tgtattcact aaaatacgac aagctttcat tcgaagaccg aatttgacat
    52681 gcaactcttc gttttctgaa tggataactg ctgtatgtcc taatcctcca agattcaaca
    52741 taccttcaca caaatcaaac gcatggtccg tgttgttagc tttgatcatg gcaagaactg
    52801 gagaaagttt ttctcttgat agcggataat ctgggcctac gccttccaat tcagcaatca
    52861 gcattttcgt tccttttggt acactgattc ctgccaattt tgcgatttcc atcgcagaat
    52921 gtccaacgat tgatggattg accgcatatt ttccttcgtt catcactgca tcttctaatt
    52981 tttgcaattc atctggtttt acgatataaa cttggtgtgc ttggaattct gcttttactg
    53041 ctgcatagat ttctttatcc acgatcacag cttgttcaga agcacaaatc attccattat
    53101 caaatgtttt cgaaacgatc aaatcgttca ctgcacgttt aatttttgct gttttttcaa
    53161 tataagctgg tacgttacct ggacctacac ctaaagctgg ttttcctgta gagtaagcag
    53221 acttgaccat tccagctcca cctgtcgcta agacgatcgc gatacctgga tggttcatta
    53281 gcatagaagt cgcttcgatc gaaggatgtt cgatccattg gatacaattt tcaggagctc
    53341 cggcagcgat agcagcatca cgtaccactc gtgctgcttc tgcagaagat ttttgggcac
    53401 ttggatggaa agcaaaaacg attggatttc ttgttttcaa cgcgatcatt gatttgaaaa
    53461 tcgttgtgga agtcggattc gttgttggag ttactccgca aaccacaccg actggttcag
    53521 caatttcaat caaccctttt tggacatctt cattgatcac accaactgtt ttatcatgtt
    53581 tgatattatg ccaaatatat tcggacgcat acatattttt gattgcttta tcttcgtaaa
    53641 caccgcggcc tgtttcctct acagccatct tcgctaatgg catatgttga tccaaagctg
    53701 ccattgccat ttcatgcacg atatggtcta ctttttcttg atcaaagctt tccatttctt
    53761 tcaatgctgc atttgctttt tctgccaaat catcaatcat tgcttgtacg tcaattgtct
    53821 tcgttttttc tttttctatt actttagcca tcattatcct cctaatgggt catggttatt
    53881 tgttaaataa ttcacatgta tatcatacaa tatttatttt tctatgtaaa cacttttttg
    53941 tgattttttt cacagttatt tttaaatata aaattgc
//
LOCUS       DAZAFF010000006        50072 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000006 DAZAFF010000000
VERSION     DAZAFF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.006"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..635)
                     /locus_tag="ACG9BB_000397"
     CDS             complement(111..635)
                     /locus_tag="ACG9BB_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFN2836371.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(628..1101)
                     /gene="tsaE"
                     /locus_tag="ACG9BB_000398"
     CDS             complement(628..1101)
                     /gene="tsaE"
                     /locus_tag="ACG9BB_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFN2836372.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1224..2204)
                     /gene="pta"
                     /locus_tag="ACG9BB_000399"
     CDS             complement(1224..2204)
                     /gene="pta"
                     /locus_tag="ACG9BB_000399"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFN2836373.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2357..3037)
                     /locus_tag="ACG9BB_000400"
     CDS             complement(2357..3037)
                     /locus_tag="ACG9BB_000400"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFN2836374.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3163..4032
                     /locus_tag="ACG9BB_000401"
     CDS             3163..4032
                     /locus_tag="ACG9BB_000401"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN2836375.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4058..4573
                     /locus_tag="ACG9BB_000402"
     CDS             4058..4573
                     /locus_tag="ACG9BB_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFN2836376.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4620..5183)
                     /locus_tag="ACG9BB_000403"
     CDS             complement(4620..5183)
                     /locus_tag="ACG9BB_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2836377.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5238..5417)
                     /locus_tag="ACG9BB_000404"
     CDS             complement(5238..5417)
                     /locus_tag="ACG9BB_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836378.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5494..6003)
                     /locus_tag="ACG9BB_000405"
     CDS             complement(5494..6003)
                     /locus_tag="ACG9BB_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836379.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6099..6479)
                     /locus_tag="ACG9BB_000406"
     CDS             complement(6099..6479)
                     /locus_tag="ACG9BB_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN2836380.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6809..7558
                     /gene="pnuC"
                     /locus_tag="ACG9BB_000407"
     CDS             6809..7558
                     /gene="pnuC"
                     /locus_tag="ACG9BB_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFN2836381.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7742..9019)
                     /locus_tag="ACG9BB_000408"
     CDS             complement(7742..9019)
                     /locus_tag="ACG9BB_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836382.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9098..9742)
                     /gene="pcp"
                     /locus_tag="ACG9BB_000409"
     CDS             complement(9098..9742)
                     /gene="pcp"
                     /locus_tag="ACG9BB_000409"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFN2836383.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9941..10885)
                     /locus_tag="ACG9BB_000410"
     CDS             complement(9941..10885)
                     /locus_tag="ACG9BB_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFN2836384.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10887..11573)
                     /locus_tag="ACG9BB_000411"
     CDS             complement(10887..11573)
                     /locus_tag="ACG9BB_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFN2836385.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12047..12367
                     /locus_tag="ACG9BB_000412"
     CDS             12047..12367
                     /locus_tag="ACG9BB_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFN2836386.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12521..13426
                     /locus_tag="ACG9BB_000413"
     CDS             12521..13426
                     /locus_tag="ACG9BB_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2836387.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13423..14196
                     /locus_tag="ACG9BB_000414"
     CDS             13423..14196
                     /locus_tag="ACG9BB_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN2836388.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14377..15801)
                     /locus_tag="ACG9BB_000415"
     CDS             complement(14377..15801)
                     /locus_tag="ACG9BB_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFN2836389.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16012..16458
                     /gene="nrdI"
                     /locus_tag="ACG9BB_000416"
     CDS             16012..16458
                     /gene="nrdI"
                     /locus_tag="ACG9BB_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFN2836390.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16523..18505)
                     /locus_tag="ACG9BB_000417"
     CDS             complement(16523..18505)
                     /locus_tag="ACG9BB_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFN2836391.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18519..19415)
                     /gene="murQ"
                     /locus_tag="ACG9BB_000418"
     CDS             complement(18519..19415)
                     /gene="murQ"
                     /locus_tag="ACG9BB_000418"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFN2836392.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19418..20479)
                     /locus_tag="ACG9BB_000419"
     CDS             complement(19418..20479)
                     /locus_tag="ACG9BB_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFN2836393.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20690..21217)
                     /locus_tag="ACG9BB_000420"
     CDS             complement(20690..21217)
                     /locus_tag="ACG9BB_000420"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN2836394.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21284..22786)
                     /gene="eat(A)"
                     /locus_tag="ACG9BB_000421"
     CDS             complement(21284..22786)
                     /gene="eat(A)"
                     /locus_tag="ACG9BB_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN2836395.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23044..24165)
                     /locus_tag="ACG9BB_000422"
     CDS             complement(23044..24165)
                     /locus_tag="ACG9BB_000422"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN2836396.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24291..25232
                     /locus_tag="ACG9BB_000423"
     CDS             24291..25232
                     /locus_tag="ACG9BB_000423"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN2836397.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25382..26278)
                     /locus_tag="ACG9BB_000424"
     CDS             complement(25382..26278)
                     /locus_tag="ACG9BB_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFN2836398.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26547..27887
                     /locus_tag="ACG9BB_000425"
     CDS             26547..27887
                     /locus_tag="ACG9BB_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN2836399.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28105..28548
                     /locus_tag="ACG9BB_000426"
     CDS             28105..28548
                     /locus_tag="ACG9BB_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFN2836400.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29052..30470)
                     /gene="nhaC"
                     /locus_tag="ACG9BB_000427"
     CDS             complement(29052..30470)
                     /gene="nhaC"
                     /locus_tag="ACG9BB_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFN2836401.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30873..32096)
                     /locus_tag="ACG9BB_000428"
     CDS             complement(30873..32096)
                     /locus_tag="ACG9BB_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN2836402.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32127..32966)
                     /locus_tag="ACG9BB_000429"
     CDS             complement(32127..32966)
                     /locus_tag="ACG9BB_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN2836403.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32983..33663)
                     /locus_tag="ACG9BB_000430"
     CDS             complement(32983..33663)
                     /locus_tag="ACG9BB_000430"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFN2836404.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33665..34738)
                     /locus_tag="ACG9BB_000431"
     CDS             complement(33665..34738)
                     /locus_tag="ACG9BB_000431"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFN2836405.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(34794..35032)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(35075..35308)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(35385..36056)
                     /locus_tag="ACG9BB_000432"
     CDS             complement(35385..36056)
                     /locus_tag="ACG9BB_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN2836406.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36143..38791)
                     /gene="mgtA"
                     /locus_tag="ACG9BB_000433"
     CDS             complement(36143..38791)
                     /gene="mgtA"
                     /locus_tag="ACG9BB_000433"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFN2836407.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(39133..39307)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            39480..39917
                     /gene="ndk"
                     /locus_tag="ACG9BB_000434"
     CDS             39480..39917
                     /gene="ndk"
                     /locus_tag="ACG9BB_000434"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFN2836408.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39939..40805)
                     /locus_tag="ACG9BB_000435"
     CDS             complement(39939..40805)
                     /locus_tag="ACG9BB_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFN2836409.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40965..43058)
                     /locus_tag="ACG9BB_000436"
     CDS             complement(40965..43058)
                     /locus_tag="ACG9BB_000436"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN2836410.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(43807..44430)
                     /locus_tag="ACG9BB_000437"
     CDS             complement(43807..44430)
                     /locus_tag="ACG9BB_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836411.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(44589..45986)
                     /locus_tag="ACG9BB_000438"
     CDS             complement(44589..45986)
                     /locus_tag="ACG9BB_000438"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN2836412.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(46018..46989)
                     /locus_tag="ACG9BB_000439"
     CDS             complement(46018..46989)
                     /locus_tag="ACG9BB_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2836413.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(46996..47124)
                     /locus_tag="ACG9BB_000440"
     CDS             complement(46996..47124)
                     /locus_tag="ACG9BB_000440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836414.1"
                     /translation="MRGSCSTEDFSYMDIDDKQHYAKNAVIIIEQQEREIKCNWQE"
     gene            47363..47788
                     /locus_tag="ACG9BB_000441"
     CDS             47363..47788
                     /locus_tag="ACG9BB_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836415.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(48276..48977)
                     /locus_tag="ACG9BB_000442"
     CDS             complement(48276..48977)
                     /locus_tag="ACG9BB_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN2836416.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(48993..49433)
                     /locus_tag="ACG9BB_000443"
     CDS             complement(48993..49433)
                     /locus_tag="ACG9BB_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFN2836417.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(49433..50008)
                     /locus_tag="ACG9BB_000444"
     CDS             complement(49433..50008)
                     /locus_tag="ACG9BB_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFN2836418.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT        14431 a        10482 c         8734 g        16425 t
ORIGIN      
        1 accgtttatt caaaaaaaat ggactgcgac acgacttttg tcacagtccc ttcgtgtgtt
       61 tttctgcctt ctcaaaagga tgacagtttc tcactctttt cctatttctt ctattcaatc
      121 aaataactca ttttatatac atctaaaaat tcacctagat ctgttcttgc ccctctaggc
      181 atgaccgctt cgatttggaa gcccattttt cggtaaaggt gtacggctct ttcattacga
      241 acttgaacgt caagctccaa tcgaaaaagt acacccattt ccttcgccca actgatgatt
      301 tcttctagca taagagtgcc tagtcccatt ccccaatatt cctgcagaat actgataccg
      361 acttctccca cgtgagacaa acgaaattga ctgtctgcct tgacagatgc tgttccaata
      421 atcgtccctt catagattgc aagcagcaat aaattattat tgctttcgta tatataatcc
      481 aactcttctt ctaaggtttc tggtggtaac aggagttctg cttcatccaa aactaaaaat
      541 tctgtctcct tattaacttg tcccatcatc aaaagaatgt cttttgcatc tcctggcact
      601 gcttcccgca agatgatccc aacttcttca ctcatgattt tcagcccatt tttctagaat
      661 tcgctgtttg aaggtttcac tttgctcccc gtatgagatt agtttcacat accgcttttc
      721 taaatctgta tcactttttt ctaaaatcag ttccaaataa tcctccggca gtgcttcttc
      781 taatagattt ccccattcaa caacggaaag cccatcacct tcaaaatatt catctaaccc
      841 taaatctgct ccgctagctg ctacccgata aatatccata tgatacaacg gaatccgacc
      901 ttgactatat tctctaataa tcgtgtatgt cggacttttg atcatttgcg ttatccctaa
      961 tccttgggca attcctttcg tcaacgttgt tttacccgca cctagatcac cagtcaatat
     1021 cagattgtca cctggcattg cggaatgacc aattgcctct gcgaattgtt ctgtcattgt
     1081 taatcctgat agttcgatca tttttcatct tctcccgctc ttctttcttt ttgccagtat
     1141 atcgcgtaat gaagtttttt taaagaaaaa atgaactagt caagaacagc agctgctgtt
     1201 tttggctagt tcgcgtgtat ttgttaatca atcaatgttt gtgctgctgt gataatcgat
     1261 aatttatata catcttcttc atttgctcca cgagacaaat cagaaaccgg tttgttcaag
     1321 ccttgtagaa ttggtccgat tgcttcaaag ttaccgaaac gctgagcaat tttatagccg
     1381 atatttcctg actgcaattc tggaaataca aagactgttg cttgaccagc tacttctgaa
     1441 tctggcgctt tcagttgtgc aactgaagga acataagaag catcaaattg caattcgccg
     1501 tcgatcatgt attgtggcgc tagtttttta gcgattttag ttgcttcaac tactttgtct
     1561 acttctggcg atttcgctga gccttttgtc gagaagctca tcattgctac ttttggttcg
     1621 atatcgaaca attctgctgt ttttgcagaa tctacggcga tttcagccaa ttcttgcgca
     1681 gttggattca cgttgatcgc acaatccgaa aagacatatt tttcttgatc tcttccacgt
     1741 accatgatca tcgctccgct tgtccgactg atgccaggct ttgttttgat gatctgaagt
     1801 gctgggcgaa cagtgtcgcc agtcgaatgg atcgctccag agaccattcc gtctgcaatc
     1861 cccatatacg taagcatcgt accaaagtaa ttcacatctt ttaaaatctt ttctgcttgt
     1921 tctttggatg cctttccatt tctacgttca acaaaagtat ccaccatttc atcccactta
     1981 tcgtaattat cagggctgat gatcgtaaag ctagatgttt tgattcctct ggcatgcgct
     2041 gcatttctta tttcttcttc gttccctact aaaatgggtt ccacaagttc ttctgctttc
     2101 aaccgggcag ctgcgcctaa aatacgagca tcagtagctt ccgggaaaac gattttgatc
     2161 ccacggcgaa caattttaaa tcttaaacta tcaaacaatt ccacaagatg acctccagac
     2221 gtttttatat gtattatcat acactattcc agaaaaaaat aacagtttat tttatctgta
     2281 tcagtcaaaa atgctggaaa aattaagttt ttcacataca aagcgaactg cactataaca
     2341 ggaaagcgct gttttattac actgtttcag gcagttgcca atcaatcggt tcttctccta
     2401 acgcaaggag cgcatcattc gttttcgaga agggtttgga accaaaaaag ccacgacttg
     2461 cagataacgg actgggatgt ggagatgtta tgatgatatg gcgactctta tcgatcattt
     2521 tgattttttc ttgtgccggt tttccccaca agataaacac gattggtttt tcccgttcat
     2581 ttaatttttc aatgatcgtg tctgttaatc tttcccaacc tttcccgcga tgagaatacg
     2641 cttgcccttc ccgaacggta agaacggtgt tgagtaaaag gactccctgt ttagcccatg
     2701 acactagatt tccatgttga acaggtgcga ttccaagatc atcataaagc tctttataga
     2761 tatttcttaa cgatggcggg atctttacgc caggttgcac agaaaaagat aagccatgag
     2821 cttgattttc tccatgataa ggatcttgtc ctaaaatcac cactttaact tcttcataag
     2881 gagtcaattc caatgcttca tagatatgat acatatctgg atggatcttt tgggacgcat
     2941 attctttttt taaaaattct cttagatgca gataataatc ttttgaaaat tctgaggaca
     3001 aaacttcttg ccaactgtta tgaatgattt ctttcaaaaa ctccacctcc taataaaaag
     3061 aataactcac attttcttca atcacaaggg acaatcttca ccctcagcac ttgattaata
     3121 tggtaaacta ggaacaagaa gcagaaagga acgataatca ttatgatcaa attaattgca
     3181 tcagatatgg acggcacatt attagatgcc catatgagtg tttctcctga aaatgccgag
     3241 gcgatacgtt ttgcaaatga tgccggtgtt gagttcatgg tcgctactgg tcgaaactat
     3301 caagaagctc gcgcagcttt agatgaggta ggaatcgatt gtgcgatgat cactttgaac
     3361 ggggcacagg tatttgataa agaaggaaat tcattgttta ccgtgtcgat tccaaataat
     3421 caagcaatct ctgttttaga tattctagat gctaacggta tttactatga agtagcaacc
     3481 aacgatggtc tttattctga aaatcaagcg aaacgaattg aaagttttgc ttccatggtg
     3541 gctacccatt tacctcacct tacgtataaa atggcgattg ccatggcctc agctaagctg
     3601 gagttattgc acatcacata cgtggacagt attcgtgcta ttttggaaga aaagaatctg
     3661 gaagttttaa aaattatttg ttttcataca gaagggccag ctgtattagg tccagttgga
     3721 aagcagattt ctcttttagg cgatctagcc gttacttcct ctggtcaaaa caatatcgaa
     3781 gtgaaccata aaaacgctca aaaaggaatt gctgttgccc atgtcgctca cgagcgtggg
     3841 atttctttag aagaagtcat gacgattggc gataacttca atgatgtcag catgctgcaa
     3901 gtagcaggag tcagtttcgc aatgggaaat gcagaaatcg aagtaaaaga ttatgccaaa
     3961 tatacaacgg acacaaacct tgaatctggc gtaggaaaag ccatattgcg cgcgattgca
     4021 gaagacttgt agttttatct agaaaagagg gatatagatg tcagaatttt tcattcagga
     4081 aaagcagctt ggacgtgcaa ctcggacaat tgtcaaaaac gaaaaaggac attccctgtt
     4141 tttgatggtt ggtcgctggg gaacaaaggg cgatgcattg tctctttatg ccatgaatgg
     4201 cgatctcgtt gctcacatca agcagataag cctgacattc ggtacgcgtt ttgagctgta
     4261 taaagagttc gaaaaggtgg gaaccatgca gaaaatattc aattggcccg gtgactttta
     4321 ttatattcgt agacttcact ggacagtcca aggtgatatc tataatcatc aatatcaaat
     4381 ccatcatttc aatcagcaaa tcatgaaaat ggataaagca acactttttt caggagatta
     4441 ctatgtattg gatgtctcaa atgatgaaga tgctccagtt tgtatttgta ttgctgcggt
     4501 tttagattac tggctctacc gtaaagacaa aagtcgcgat aagcctgttt cttaccgact
     4561 taacttaagt taaaaagatg tacctgaaat gcggaaatac gcatttcagg tacatctttt
     4621 tagatattct tttgcatatg atcataaaga tgaccactga tcatccgctg accgacaact
     4681 ttgaagtcat ggcgttcgta taatttttta gcctttggat ttttttcatc cacacttaga
     4741 ccgatgacgg agcgattagc tttcttcgcc aatttaggta atgcttctaa taatcgcgaa
     4801 ccgatgcctt gtcctctaaa atcttctgat acactgatag agtccaaata ccattcgttg
     4861 ggaaacgttt ctttatcaat gaacaaacgt gtagacgcat ctagctgata tttctctaag
     4921 ataggtacca gtggctgatc gatgattgat tcttcttcat ctgtataacc gtaagcaact
     4981 cctgcaactt taccgtctat ttcttctacg atacctcgtg aataactgta acgataattt
     5041 ggatcagtga ttgcttcttc taaaatctct aaagtctttt gttcccctgt ctgcttcaaa
     5101 aatggcagtt ccatatcttt tagaatcact aagatcaatg gggcgatttc tggagcatct
     5161 tcttttctcg caaaacgaat catagtttcc tctccttttc tccattctaa catgcagaaa
     5221 aagaaatcgc aaaatcgtta ttttaaaaaa tcttcaatct tttcagtaat gaaataacca
     5281 tgagtaacga ttcttgccgc ttcagttcgg caataagggc agacgactgg catttcttta
     5341 tcattatgtt cgactacatt attgacttct tcgtatgcaa atttttttcc gcaattaggg
     5401 catttcattt tttccatgtt atctcaactt cctttcatct ttactctctt agcttactct
     5461 tatattgtaa aaaagagaag caatatcctt cttctatgca gatatccata cctgacggtc
     5521 ctgttctcca tctattaatc ggactttata ataaggacgt tcatctttga ctagaagtgt
     5581 ccactctttc acttttgctt ttttcccgta gtagctgact gcttccttca tcgctgcttg
     5641 cggttgtttg aactcttccg ttttttctat atcaaaaaga tgttctgcgg ccttttccgt
     5701 gtcatttcgt acggttgttt tcccagtgga tggattgact gctatgactt gtccttcaca
     5761 aaaaaactgg tattcatatg ttgcattcgt aggattctcc aaatcttttt caactgcaaa
     5821 atgaatagca gatatgttat gttcttttgt ttcgtttttt acgattgctg ctgctttttt
     5881 caatgagatc cgatattcct ggtcactaat atcatccatg tgaaaagcgg cattgagcag
     5941 tcccgtcata ccggtaatcc ctattacgat gagggcagca acaccaaaca tcattttttt
     6001 cattcttcat tctcctgttt ctttttttta aagaatatca agaaatgaac gaatgaaaaa
     6061 tcgaatgaaa cgaaaaaaaa gtgacaattt tgttatcttt attcgtgcag ttctaatggc
     6121 aatccgtctg gatcaaagaa aaacgtcatt ttttttcctg taaaggtatc ttctctaatc
     6181 ggttctgatt cgatccctaa ggactgcaat tctgcaattg tttcttcaat atcgtctact
     6241 ttgaaagcaa gatgacgcaa gccacaagct tctggatagt ttggtcgttg tggcgcatta
     6301 gcaattccaa aaatctccaa ttcagcactt cctagtttca gatctaattt atagtcatct
     6361 cgttctttgc ggtaattttc tctgattact tcaaatccta atttattaac ataaaaatca
     6421 cgtgatttct gataatctga aacaatgatg gctatatgat ggatcgttgt aaggttcata
     6481 tgagcactcc tattcttcgt ttaagctcgg atctatatta atctccagtt ccattgtaaa
     6541 tgtgtcagat tgaaaaaaat agctgttttt cctttttata agaaaaaagg caaaaaaaac
     6601 agaaagtagg tcctccaaga cactcttcct gttcccaata taataaatga aagtaagaaa
     6661 ttatattttc aagaaaacaa acagaaaatt atcataacac aaattattta taattgaaag
     6721 actagttttt tttcaagaaa agcatacaat agattgtgtt agtaaataca gaacacacta
     6781 catttagtgt cttaaggagt cctcatacat gaagtcatat tttcagtttt tagctcatca
     6841 gctaaaaggc tggccgcaac aaaattatta tcttttcttt ttcagtcttg gctgccaggt
     6901 catgactctt gtcaatagtc ccatcacttg gctgtccgtt attacattta tcggtaccac
     6961 gcttggtgta ttgtgtattt tatccatcaa tgcggcaaaa tcagtcaacg gtattttagg
     7021 tattttgtct gcgatttgtt ttattatcgt tgggttttca gcaaagaact atttaagtat
     7081 cggtgaacag attgcttata tgatcacatt ggatatcccc gtattgttaa gtaaagaatg
     7141 gaatcataat atggcttcaa aaatccgaaa attcactgga aaaagctggt ttatcgcaat
     7201 catcagtact ttccttgtct atcttgtttc aggattcctg attggtcgtt atacagatga
     7261 tcctcggccg tgggtagatg ccattgcttt ttctattagc ttgactgctg gtatcatcag
     7321 tttcttgcgt tacaacaacc aatattactg gtggttagct tcagggcttg ctcaaatggt
     7381 cctttggtat atttcattta cgcatggatc tgcaagtttg gcgatgttcg tgaacagctc
     7441 tgtttacttg ctgaatgatg tccttgcatt taccgtttca ccttggtata accaaaaaga
     7501 acgtgaacgt atgcaaaaaa tggaagaaga atactacaac gcacaacaac acgcataaaa
     7561 aaactgagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
     7621 cggcggaatc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc
     7681 aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca gcctctattt
     7741 tttatagtaa ttccttcaga tactttcttg cctcatcatt gaaacgcagt gattcttcta
     7801 acttctgata atattccaac gcttgtttct gagcttcagt aaaacgataa tgttttcctt
     7861 tccatttgtc gattgtctct tgcacatttc cagattggat atctttcact actagttgcc
     7921 atttttcttt tgagactgta cggtctttct tatcagcaga ttgtatagct tctcgttctt
     7981 tacgcaatac ttttaaaaaa ccatttctga ctatccgagg aatgctgtcg aagaaccctt
     8041 ctgtaaaacc atagcgacaa tattgcttcc aaataaatag atagattttt ggttggatga
     8101 atcgttggtt tttcgcaaaa tattccatct gagcagtgac ttctggtgtt cgttgacgtt
     8161 ttgtctttct caacacggaa ataaatgttt caaaataagc attaggatgt actggcactt
     8221 cttcatatac gatggattgc caatctttac gtaaaaatgc gcgatagttg atcaagaatt
     8281 cttctacatc ctttactaat tcttctgcaa agtcgtcaaa tagttctttt tttatctgtc
     8341 gcttttttga ctgcccattg atgtgttcac ttattttctt gatttcattt tccaatgctt
     8401 gataatccgc tatgtaaatc ggcatttcta cttggcgaaa ttctgcgagc cgctgagcta
     8461 attttatatc cggtaaatat ggctgtttca tccgacgatt ttggtacaag acaaaacagt
     8521 atttttgcca tagtccttca ggaaaatcga tattcttctg cttaagccat aataattcgt
     8581 ctaaggccag atcaactttt gtcattccct gatggatcga agctgccact gtcggagaga
     8641 catgcaggtg atcggcaaag tggttgatgc ctagttttag gatctttccg gtttttggcg
     8701 atttaacgat gtattgcact tttaattctc taccacactc acaatacaat ttttgctggc
     8761 ttcgttttgt tcgaaaaaac ggattgatct tgaagtcaga aaacacccaa tctgttgcag
     8821 ctaaataatg ttcttccata aaacgagagc tgattagata tcggcggtgc ttatcgacta
     8881 gttcccgctg ttttttcgtt aattttgcat agatctccca acgttgactg cttgtgtatt
     8941 gtttagaaat tttgatggga tagcttttgc tgatttcttt tggaaaccat tcttcaaaga
     9001 cggattgcca ttcttccacg gctgtttctt tcctttctaa ttacaaaaag ctggctacta
     9061 acataacgtt agcaaccagc cagtgtttct tgctaaatta gtgagttgtt ccggcatttc
     9121 cggaaatatc ttctttattt tctactgctg cttcaactgc ataagtcaaa gaagaagcaa
     9181 tgtcttctaa agacatagaa ggcgtaccca ctggacgatc gatcacttga tcgggtgaga
     9241 atggcacgtg gatgaaccca cctcgaattg ccgggaattg tgtttcgatc aaatgaagca
     9301 aacgatacat gatatcattg cagacaaatg ttcctgctgt ataagagatg tacgctggta
     9361 gaccatgatc ttgtacattt tttgtcattg ctttgattgg caatgaggta aagtaagcgg
     9421 ctggaccgtt ttcttctaaa gccgtatcga atggctgatt tccttcatta tcagggattc
     9481 ttgcttctgc tagattgatt gcgacacgtt cgaacgagac tgctgaccgc ccacctgctt
     9541 gtccgacaca gatcacgaca tctggttggt gctgttggat ggcttctttg actacttgac
     9601 tgcttttttc gaatacggtc ggtacttcca ctttgatgat ttcagcaccg ctgatttcat
     9661 ctggcatttt ttttactgct tcataggctg gattcacttt gtccccacca aatggatcaa
     9721 aacctgttac tagtactttc atctgttgtt cctccgtccg attgatttaa catatggtta
     9781 tcttttatca taccattttc cttgaacagc gtaaataaag gaaacaatag tttaaataac
     9841 ataacagatc aaacttaata tattcttata aaaaaatagt gttccactac aatgttagaa
     9901 gaggttgcga caattattct gtcacaaccc ctctcttcta ttatttaaat agaatcatat
     9961 aagcaatttg gaaaactagc ataaataacg caataaacaa ctggtttttg atcactccgt
    10021 atttgtcttt catctccagc atcgctacag gcacgatatt gaagttagca gccatcggtg
    10081 tcagcagggt cccacaaaaa ccgcaagtca atgcaaccat tcctattaac gctggattcg
    10141 ctccatgact aaatacgaat ggtgcaccga tcccaaccgt catcacggtg atggccgcaa
    10201 atgcattccc catgattacc gtaaatatca ccatgccaag agcgtaaatg atgatgccga
    10261 tgttcacatt tccttctgga acgatcgtac ctactgcatt ggaaatcact gtcccaactc
    10321 cagcgcttgt aaataccgca cctagagaag ctaaaagcat tggcaacatg cttaacggcc
    10381 ctactgtgcc cagcatatct gctgcatcat ctaaaaatgt acttggtcga ttatctcgag
    10441 aaaagaacat caagattagc atggctgcaa aaacaccgac gcccacgccg actaaagcac
    10501 ctaaattggt aaatagagca aacaagatcg caaagacacc gatactcaaa gctggaataa
    10561 aaattttcat tcctagtttg tctgacattt tttccatata ttcttttgat ggaagccggc
    10621 tttctccttt gctgactttc ttagcaattg ctggtatggt catcgcaaag atcaagccac
    10681 cgttcacgat tgccggaagc catcttcctc ctgcaataac tacccctaag accgtccaga
    10741 aaaaggctga cccataacga tgaggattgg ttttatctgt taaatttctt actccagtgt
    10801 aaatcaatac gagtcccatc acgatataaa ggatctccag taatttatcg cctaataaaa
    10861 tatcaggatt tgtaaaaaag tccatcttat tgcccctttc ctggataata tttcttcgct
    10921 aatttctttt ctttaagata gaagtagaca cttgccacaa ctaaagccgt gatagccact
    10981 ggaacttcca ctaaagcaag ctgtcccaat gttacgtcat atcctagatc tttcagtgtt
    11041 gattggacta gcaacgcccc tgctcctccg atgaatagta cttggccaaa gaaccagcag
    11101 acattttcca tagctgaagc catacctttt agttcttctt catgcttagg atggacttct
    11161 aacccttttg attcgatggt ccccaatgcc atcggtaaaa tgattggacg aacaaatcca
    11221 gcaactccac caaaactcac gttaaaagcc gcaaagatca ttcgaaagat cccataaata
    11281 tctacaatca taccagcaga tactttcttg aatcgcttga tcaaggaagc cgctgattct
    11341 ttcagaccat ttctttcaag cgttcctgtt gccaacataa tgatgataaa aatcgccatc
    11401 ccgcgattgt ttacaaaact tgtacctaaa agttctaaca ttccttgaat gccaagaccg
    11461 ccagtcaaag ctgtaacaac taaagcaacc attacgatca atatcgagtc taatttaaat
    11521 gcaaacccga gaataacaat caagacgcct aaaaggctaa gataattttc catatatttt
    11581 cctccataaa atgacaatat ttttttaatt ataaagcaaa acacataaaa aatatagttg
    11641 ttttctcacg aaatagacac tatttttaaa tatttttcgt tattttttta tcacaaaaat
    11701 tatcttactt attgtaaggt aaagcttttt ttaatttatt gcccgataat atatagaaat
    11761 aaggtctagt tactgttttt tttaaatgat taataaataa caacttcttt ctatgaaatt
    11821 gcaaagcatt catcttcttt tccgctaacg atatatccgt cagtgcttgg tacactttac
    11881 taaaaagtat gagattgccg atatctggat aggtgtgcta tactacagaa aaagatatac
    11941 aatctgccac cgggtaggat aaagaggatg atcattccat taggtctttg aagggatgat
    12001 tttcctcttt ttgtgtattt ttttatagtt aaggaggaat gtaagtatgg catggatgga
    12061 attaatcata gcaggaattt tggaagtatt ctggtcaacg atgatgaaat ggagtgatgg
    12121 tttttctaaa atcaattatt ctttttacac agtgattggc atgattgcta gtttttactt
    12181 tttgtcaaag gcaatcaaaa gtttgccgat gagtctctct tatcctatct ggacagggat
    12241 tggcgcagtt ggttcggtca taattggtgt ggtcttgttt catgataagc tgaatctttc
    12301 cacttggttc tttgtgggga tgctactcat cagtatcatt ggcatcaaga ttactagcgg
    12361 ccactagaca cttggaataa gtactgatca gaaaaaataa gcctttttat ttaatattca
    12421 atttttttat gaaaaaataa gaaataagtc caatgaccta gcgacttctc cactcatcag
    12481 gtgtatgctt gtactatcta tagaaaagga gtgaatattt ttgaaaagtc tttcggtcca
    12541 agaattatcc aagaactttg gtcacaaaca agccctcagc gatgtcagtt ttagcttatc
    12601 ccctggcaaa atcgttggat tagtaggtcc taatggcgct ggaaaaacaa cgatcatgaa
    12661 agctatttta ggactgatcc attactcaga tggaacaatc tgtattggtg aaaatgagat
    12721 ttctccctct agtcataaag ggttggaaca agtaggagca ttgatcgaat acccgggtat
    12781 ctatccattc ttaacaggct atgatcatct gaagttattc tctgaaacca acgatgtatc
    12841 tgctatcgac actatcgtga atcagctcaa gatggaaaag tacattcaca aaaaagcgaa
    12901 atcctactcg ttgggaatga aacaaaaatt gggaattgct cttgccttac tgaaccatcc
    12961 gtctatcgtt attttagatg agccgatgaa cggtttggat ccacaagcaa caagagatgt
    13021 gcgaaaaatc attaccgact tggctactca gggcacctct ttcttgatct ccagtcactt
    13081 actcagcgag cttgaaaaaa tggtcgatga gctgatcctg atcaatcaag gaaagatcat
    13141 caaacaatgt acaatggctg aattgaacca aacagcgcaa gatttcatcg ttatctctac
    13201 aacaaatgat ctagctgctc gaacgatctt gacagaagct ggatatactt tggaagatcc
    13261 tgataaaatc aagcttttaa aagaaaacga agaattatta ggaaccgtga tccagctatt
    13321 gtctgcacaa aatatccatg tgacagacgt ccagcatctg acgaatgatt tggaatcttc
    13381 tctattggaa attctggatt cagtaaaggg ggaagaagaa aaatgacatt gcttgtaaaa
    13441 caagaattat ttaaattgat caagaaaaag tcaactgctg ttttatctgt tctattagtt
    13501 gttttgctca taggaacagc acttctagcc aaaaaataca cgacgatcat tgatccagtt
    13561 gaaatgactg ctcagctttt tagtgccaca tcttggattg tctttatcat gattgcagct
    13621 gcaagcacga tcatttcgat ggaagcacaa tatggtacat tgaaaaatct cctttatcga
    13681 aagtattctc gaggagaaat attagtaagc aaatggatca cattagttat ttattctgtt
    13741 tatttatacc ttttagctat tattgtcact gtgttgatga aactgattct tttcccttcg
    13801 atttccttca cggagaaggt ttctactggt caaacactga tccaaagctt gttcacttat
    13861 actttgggta gttatatcgg tttatggctg attttaagtt tagtgttgat gattgcttgc
    13921 tttatcaata gttccggcgc atccatctca gcaggaatcg tcttttattt tgcttcgtca
    13981 atcatatctg gtatccttat tgcactcatc caaaaatggg agtggatcaa atggaatccg
    14041 atcagtatgt tgaatctgca aaaccaaatt ggaaacgaag agatcatgaa gcaattgact
    14101 catctttcta ctaatcaaat gctattcggt aatttagctt acatcgtttt gttcttagca
    14161 ttaggatatc tcgtatttaa gcgaaaaaat gtatgatgac tcatcaaaaa agaggctgtg
    14221 aaagagacgt tcagccttat tgccgattgg ctgtctctta aacaccgttt attctgaaaa
    14281 aggagctgtg acaacatttg tgtcacagct cctttcctag tatgtttttg aataaacgac
    14341 aggctcacaa cctctcttta tacttctttt atacttctat acgtgaagca tcccagtggc
    14401 aaataaaacc attgcaatca ctccgaccat cgtcacacct ataagtaaaa tcttatctaa
    14461 gtggatatgt tgtacaggct cgttttgctc cttacgtgct ttgaggtaaa aataaatgcc
    14521 tggcaagaaa agaacaagaa caagcattac ttcattaaag gctacgacta gcatagccca
    14581 tacttgaaag gcacttgcta aaagccctac taaaaattgg acccattctt tccgctgata
    14641 agaaagtttc atttggtaca atcctaagaa aagccacgtg aagaaaatag ctgctgtacc
    14701 aagtgtgtaa gcaaagttat aagcactgtc agtgaataat aatgaaagta aaaagacatt
    14761 cgtacataac gtcgtcaata cgatagcata agttggtgcc ccatttttgt tgagttttcc
    14821 ccatgcttta ggcaaaagtc cgtcttcagc catgtttccc attgtttcaa tcggtaagat
    14881 cgtccatgag agccaaacac caactaaaga aatgatcaaa ccgccgttga tcacgactgc
    14941 tccccattta ccaaccatca tttcaaacac atagcccata gcaggctgcg ccttgatcgt
    15001 ttgcagctga ttcatcgtca ttgtcccata tggaagcatg gaaaccaaga cgtaaataat
    15061 aatcaagccg aatatcccta gaatcgttgc tttcccaaca tctgattgtt tttttgctcg
    15121 tgaacctacg acagaggctc cttcaatacc aacaaatacc caaagcatca ccatgatgca
    15181 actcaatact tgactggaaa acgggatcgg ctgtccattc gcattcaata taatgtcgct
    15241 agaaaagtta tcaatgaatt gttggatcga aaacgtgctg atagctacta caataaataa
    15301 aaataatggc actaatttac agaatgtgac gatagcattg acgaaactag cattttcgac
    15361 tccccgattc actagccatg catatcccca taaaaagact gtactcatca cgatcgatgc
    15421 cagattttgc ccaccttcaa atactgggaa gaaatatcct aatgcgctca tcaacaatgt
    15481 tgcaaaagct acgttgccta accagcatgt gagccagtaa ccccaaccgc tgataaaacc
    15541 agcgaaacgg ccaaaacctt cttctgcgta actaaagatc ccgcctttta aatctggacg
    15601 ttttttattg atattattga tcgacaagga gagcatcaac attgctgttc ctacgagtac
    15661 ccaactgaaa atagctgggc cgccagccga tccggagatg tccgtcataa taccaaatac
    15721 gccacctcca atcgctgaac caattactaa ggcagcaagt ggaattaaac caattccttt
    15781 tttctgttgc tctgtatcca tgtaagcatc ctccaaaata aaaccgcttt tatcaaaaag
    15841 tataacaaac cttttttatt tttacttaat taataaaaac tttaatagaa atataattct
    15901 accggctttt ttctcattat acaagcaaag cgcctagtcc ctttccaaat tacaccacca
    15961 aaaaacttaa aaaagatgct atactaaaag gaaagaattg gaggatgatc catgaacatt
    16021 ttatatatat cgatttcagg aaatacgcgt gcctttgcca aacatctagc agaatatgct
    16081 gaaaaaatgc atgcagaaga tcctgtcaat cctgaagtta ctttaaaaga aatccatgaa
    16141 aatagtgatt ttacgaaaga aacagaacca ttttttactt tcgttcctac ttatttagac
    16201 ggcggaaatg gtttagataa cggtgataca gaaatcttga ccgaaacgat gcgtgaatat
    16261 ctggaacatg aagacaatca taaactttgt cttggtgtag tcggtagcgg aaataaaaac
    16321 ttcaataatc agtattgtct aacagccaaa caatatgcac aaacatttgg ctttcctttc
    16381 ttggcagatt atgaattacg gggaacacca tctgatgtga cacgaattta tcagatttta
    16441 gcagaaaaca ataaataata aaaaagaaga agctgaaaaa tagatttcag tttcttcttt
    16501 cgtttctatt gtttctatca tattaagctt tgacatccat cacaggtgct tttgcttttg
    16561 ctttacctgt tttttccaag acaaagtttt ttacacgatc catattggtt accaagacga
    16621 tcatggatgt ttctttgcct gctgctttga tcgcttctaa gtcagcttct gctacaagcg
    16681 tatcaggtgt cacttgctgc ccttctgtta ctttcacatc aaatggcgta ccgcctaagt
    16741 caactgtgtt gattcccata tgaagcaaga tttctaaacc atttggcgtt gtgatcccaa
    16801 tagcgtgttt cgttgggaag acagagctga ctaccccagt aactggagaa tagatatgtc
    16861 cgttttctgg ttctacaaag tagccttcac ccatcatctt ttgtgcaaaa actggatcac
    16921 ttgctttatc taaagaaaca actgttccgt cagcaactga tgagaggata gtcacacttt
    16981 cagctgaagt ttgaggtact actttttcag gcacttcatt tactgtggct gtttcagtcg
    17041 ctaattcttc tttttctaaa cggtctttag gaataccgaa gaagaatgta gcaacgaagc
    17101 caccagcata agctgccaat aatccaagga tgtagcctaa ccatttaccg tcagagatca
    17161 atgggatcaa agcggctcct gaaggtccaa tagcgatagc cccgacattt ccgatcattc
    17221 cgatcactgc cccgccgata cctccaccga tacaagcagt aataaatggg cgtcctagag
    17281 gtaatgttac tccgtaaatc aatggttcgc cgattcctag gatcccgact ggtaaagctc
    17341 ctttgatcat ttctgttaat tctttatctt ttttgcagcg aatccataat gccaatgctg
    17401 cacccacttg tcctgcacca gccatcgcaa gaattggaag caatagcgta cgaccagttt
    17461 catcaatcat ttgcacatgg attggagtca aaatttgatg cagtccgaac ataaccatcg
    17521 gcaagaataa tgtgcctaga acgaagccgg caaatgctcc accaacgcca agtacccagt
    17581 tgatcccacc gaccatgcca tcagaaatga aacctgctaa aggcatgatg aagaaaattt
    17641 caatcagacc gatggcaatc aatgaaagag ttggtgtaac gatgatatct accgcatcag
    17701 gtactacttt atgcagtttt ttctcaacga ttgacaatag ccaaacagcg aagatgacac
    17761 ctaggatccc accttgtcca gctgcaagag cttcaccagt aaataagttt ttcagtggtg
    17821 cttccggatt catccctgtc agcatcacaa cggccccgat aactccacca agagttggtg
    17881 ttgcaccaaa aacttgtgca gcattgatac ctgtatagat aactaggtag ctaaacatcc
    17941 cgtttttcaa gatgttcatc acatcgatat attgttgcca tgttgctgca tcaagtgttc
    18001 cggctgtgac taagtttgct aaaactgcgg caatccctgc cacgatccca gtaccgacaa
    18061 atgcaggaat cattggcaca aagatattag aaatgtcttt taataccgat ttgattttag
    18121 atggtttttg tttcgatttt tgtgccgctt tcatttcttg tgcccgttcg tttacacggt
    18181 cttttccaga tgctgatgaa tttgctgtag ctgtacttac agaagaaagt ttttctccta
    18241 atttcacgcc tgccaaatca accatttgct gtgctacttt gttcactttc ccaggtccga
    18301 tgatgacttg aagctgttca tcttctacta caccaagcac tcctggaatc gcttttaatt
    18361 cagagacatt tacaagatca tcattgatga tcttcatacg cacacgagtc atgcagtgga
    18421 caatgctttc tacattgccc atgccgcctg cccctttata aatttgtttt gccagttctt
    18481 ccaaagtcag ttcgtttttt gccatggtct tttccccctc ataaggtccc ttttacaaat
    18541 ccgtttgctt cagctaattt tgcttgagct tcctctttat cactatttgt caaaatcatg
    18601 acgatagcca gcttaacatt ttgatctgct tcttcaaagt acttttcagc tgtttcgtaa
    18661 tcagcttctg tcgcttgcat gatgattcgt tttgagcgtt cgattagttt ttcatttgtt
    18721 ggtttgacat cgaccatcaa gttattgaat actttaccta taccaatcat actgatagta
    18781 gaaatcatat tcaagataag tttttgtgct gttcctgact tcaatcgagt agagcctgtc
    18841 aaaaattctg gacctgcatc gacttcgatt gggaaatccg cgtgtttact gatttcggct
    18901 tgtttattgc aagaaatcgt tcctgtgaca gcaccgatcg attttgcgta atctaatcca
    18961 ccgatcacat atggtgtacg accgctagct gcgataccaa taactatatc attttttgtc
    19021 aaattcagct tgattagatc ttcttgccct agctcataag aatcttcggc cccttcgaca
    19081 gctacagtca ttgcttcttc tccaccagca attagaccta tgaccatttc cggttctaca
    19141 ccgaatgtcg gtacgcattc agcagcatct aacacaccta gtcttccact tgttccggct
    19201 cccatataaa tcagacgtcc acctttttta aaggactcaa tcgtgttagc aatcactggt
    19261 tcgatacttg tcagttcttt ttcaaccgca gcggccactt tctgatcttc tttgttcatt
    19321 agttgaacgg cttgagaaat actcatctca tctaatccaa aagtttcttg gtttctgcgt
    19381 tccgttgtta ggttctctaa attgattttg ctcattttta tcttccttct tttcttataa
    19441 attgatagtt cataccagct ttgatttggt tgatcaatgg tagctcttct ttgataactt
    19501 tagctactac attcactttt tcatccgctg gcaaatcata tttggttaat tggatctcac
    19561 ccatatagcg caaatacttt tcgttatcta aagtaataga ccctttcata cgcgtagctg
    19621 tatttcttgc tgggataggc ggaatctctc gaaaacgtgc atctacgctt cgtatgacat
    19681 ctcttgcatc gtcttgccga ttcgtatgct tccctaatat atattcgtaa aattcttcat
    19741 ctacaggttc cgtatggagc aagactgtct gttctttttg gaaagaagca aattgctcct
    19801 gaacgttttc tgacagacct gcatctccga tgtaaacaat atccgtgtta cttgctaata
    19861 gatctagagc gcctgcaaga ggatggatcc ctcgatgttc ttccaaagtt ggcaatcctt
    19921 gatatagtgg gcctctcaac tctgtatctc ctggtacaaa accttggaca aggagacctt
    19981 gaattttgag ccattgattt tttttctgat accattcttt gtccagccct gtttcgggac
    20041 gtggataata attatgccaa gcttcaatct gtgagaaatc agcttctgct tctttcaatt
    20101 catcgatatc tttaggcgtg atcgtgctag cattcaaact gattttcaat tgatgtgacc
    20161 agtcggcaat ctgctgattg gaaatatgat aatccattct taaacctgtt acaccaattg
    20221 ctttcaaagg cgttagatct tctaatgaaa aacccgctcg ttccaatgcc tctcctgaaa
    20281 tatcgaccat caattctagt ttattctttt tcgcttcgct tcctagttcg atcaatcgct
    20341 tttgatatgc cgaaacatca tcttctggta tatgcatgga agtgaaaata ccgacaaagc
    20401 cattggatgc catttggcgg atatactgtt tcttctcttc tgacaaagaa tcgttcataa
    20461 atattgaaaa gccaaacata ttatccctct ttttctgtat ccgtcttcat ttctatgact
    20521 agagtatata tctaaagaaa caaaatttca atacttttat taaaataatt aaatttcatt
    20581 tctaaaggtt gagtttatat tatcaaaaga attgtcataa cctaaaaaag gcactgctaa
    20641 gcaaaagcag tgccttgaaa tattttattt aaataacaca aaaacataat tactcataag
    20701 aaaccaattc tgtattatct accatgaatg taaagaaccc ctcattaggc atcggatgaa
    20761 aataagcatt gatcactctt atcaaggcat tatggcaaac taacagatag acattttctt
    20821 cgtcttcgat gcattctttc aataaaggaa cgatccgtgc atacacatct aagactgact
    20881 ccccatttgg gaatcgtaca gcgaacgcta aacgtgcttt ttgaaagttc tcatctttgc
    20941 ttggcagtcc atcataatcc ccaaaatcca tttctaccaa tcgttcatca acggtcagcg
    21001 gcaagctgag acgatcagca actgcttgtg ctgtatctct tgctcgttga agaggggaat
    21061 ggatgatttt cgtgattgga acaggcaaat ctactacctt ttctgctagt ttctgtgcct
    21121 gcatctgtcc tttttcagtc agcggcacat ctgcccgtcc gcaaactaat ccttgtgcgt
    21181 tccaagttgt ttctccgtgt ctcgttacat aaagcatgtg attctctcct tcgttggcaa
    21241 agttcccctt aaccatacct tgttgttctt gagattacaa gggttagatt ctctcaagag
    21301 aaataatctc agtagatact ttatccagga aggtttgatc atgttccact agtaacatgg
    21361 caggtttcac gttcaagatc agttgttcta attgttcttg attgaagaca tccaaataat
    21421 tcaatggttc atcccatgta tatagttcag ctggctgtga taaagattta gccaattcca
    21481 cttttttccg ttggcccata ctcatctgct cgatcttgtt atgaaaaaca tctctttcca
    21541 tcccaagctt tcggaggttg ttcaaaaatg cttggtagtc tacttggttt ttctctgcaa
    21601 attccgccaa cgttcctcga ttgtcttcat aattttggct tgcataacta atgctcagat
    21661 gttttggctg gtatttttct cctataacct tgccgttgaa tgcccccaga agataatgga
    21721 taatggatga ctttcctgca ccgttaggac ctgaaatcgc cacccgctga tgaggctcga
    21781 ttgtaaaatg aattggctcg aataacagat tttcataccc taattgaaga tcttctacgc
    21841 ttaaaagtcg cttatggtga gacgcttggc tattcatcgt caaagaatcg atatattcga
    21901 tatcttttaa tagtttttct ttctcgctga tctgggtctc catccgattc acgattgctt
    21961 tggatcgttt catcgtccgt gcagcatcag cacccactgc tcctttattc actcgtctag
    22021 attcagtatc gatgaatccg acttgtttct ttgttttatc tccttctcgg gaacgagacc
    22081 attcggcttt ttcagctgct gttttcttta gcctactgac ttcttttttc aatttttcat
    22141 tttgagccat ttcaaattca tcacgaagtt ttttctgttc ttcatagata gagaaattcc
    22201 cttgataaag ttccagttga cttttttcga ttgctaaaac atggtccact acttcatcga
    22261 taaatccccg gtcatggctg accacgatga agccttgttt tttctttttc aaataagccg
    22321 ctacttgttt tctaccagag atatccaaat gattcgttgg ttcatcgatt aacgggaaat
    22381 gagtgtcatc cacaaataaa agtgccagta ggacttttgt cttctctccc cccgatagtg
    22441 tgctgaattc tctccataag atttcaggat ctgtttgcat caattggagc tctctttcga
    22501 tttcccatat ctcaaaatcc gtaatatcat ttaacacgta ataggttaaa cgttctttat
    22561 cttttgtctg ttgcgggaaa taggcaaatt cttgctgatg gattacttgc ccttgataag
    22621 gtagtttgtt ttgtagaata ttcagtaaag ttgtctttcc tcgaccgttt cgtccgataa
    22681 gtcctagttt ccattgtgtg tcaaaattta gatttgcttg ttcaaataat aatgtgcctt
    22741 ggctgtcgta gccgaatgtc agatttttta tttcgatttt agacattttc catcacctcg
    22801 taaaaaaaga ccagtcctat tttcagaact ggctaaagag gatgcaaggg ttggatacac
    22861 acaaaaagac ccatacatcg aaaaaaccaa gccaatcccg aaaatctgca cactgcttcc
    22921 ttgtctccac aaggtacagt ctgaatgcag ctttcatcaa tcgacttaat ttttcaatgc
    22981 acggatccct cactttgtcc tatatttgaa attacgatag cataaagaaa atgattttgt
    23041 caactacttc gggaggatgg tgaaaaatct gctaagtgtc tgtcctaaga tttttgtttt
    23101 ttctaatgtt tgcctgtgtt ccattgcttc ttctaaacct attggaccca aatgaatact
    23161 taagacaatt gggagaaaat cagaaagtcc ttctagctct tggtctctac ttccgcaaat
    23221 agcgattatt ggttttcgat atttttttgc aagcactgct acaccgtatg gaacttttcc
    23281 gtggatcgtt tgctgatcga tccgcccttc tcctgtaatg atgacatctg cttgttcgat
    23341 ctttttttcc aatccagtga tttggctgac taaatcgaaa ccagaaacca tcttcgctcc
    23401 caacaaaaaa agggctccac caataccgcc tgctgctcca cttcctggaa tatcatctaa
    23461 atccacctcc cagaaatctt ttatttgctg agcaatcttt cctgcttgtt tatctaagaa
    23521 agctagcgtc gtatctattc ctcctttttg tcttccaaat tgccaagcag ctccttgttg
    23581 acctgtatat ggattggtaa catctgcagc gataatcaat tttactttag aaacaagaac
    23641 gtttcttcga tcaaaatgaa gttttttagt agataataaa ggatttcctt ctgtgtaccc
    23701 ttctaaagaa gcacccaagg attggagtaa tcctagacca ccatcgctac acccgcttcc
    23761 tcctaaagtg acaataattt gttctacttc ttgaagcagt gcatctttaa taagttctcc
    23821 taaggcataa ctggaagcaa gtcggatagt ttgatccgtt ggtttatcaa gtagatctaa
    23881 ccccaatgct tgtgcagatt cgatgactgc ggtgtttttt ccttgccaag ttgtcaataa
    23941 atagctgatt tttcgctgac ggaataagag atccatccca tcaatggtac gccatgttcc
    24001 cctgagcgcg taatagattg cttctgcagt attttctccg ccatctgcaa tcggcacatt
    24061 gatttttctt cctttacgat cttcccagcc atttagaact gcctgattta gttcaaatga
    24121 agaagcactt cctttaaatg aatcaattgc cgccaaaata tccatctgta tccctcactt
    24181 tcttcttaag taagacactt tttttcattt ttttcaaaca aaacaatgct attttcttcc
    24241 atttttctat atactaaaag ggaactcatt gaataaagga gagaaaaaac atgaaaatcg
    24301 caatagctgg ggccggagca atgggcagcc gaatcggatt gatgctacat caaagcggaa
    24361 atgaggtcct cttgatcgat cgttggcctg ctcacattga agccattcgt acaaatgggt
    24421 tgattgctga ctttaacggt aaagaagtgg tcgcaaaact gccaatctat tcaccagaag
    24481 aaatcatcga aagcaacgaa cacgtggact tgatcgtagc attaacaaaa gccaatcaat
    24541 tagatgatat gttttgttcg atccaatcca ttattactga caatacttat gttttgtgtt
    24601 tactaaatgg attaggtcat gaagatgtac tggaaaaata tgtgccaaag aaaaatatct
    24661 tatttgggat cacgatgtgg acagctggtc tagctggtcc cggaaaagtc acattgttag
    24721 gtgatggaga aatcgaacta gagaacttgg aaccagaagg cgaagcattt acaaaaaaag
    24781 ttgtagaagt ctttcaagaa gcaaatttaa atccgatcta tagccataac gttcgttatt
    24841 ccatttggcg aaaagcttgt gtaaatggaa cattgaatgg tttatgcacg attttggatt
    24901 gtaatattgc tgaactaggc gcgcaaaaag cggcagagag catggttcga acaatcgtat
    24961 ctgaatttgc ttctatcgca gcaaaagaag gaatcatact agatcaagaa gaagtgtacc
    25021 aacatattgc ttctacttat gatcctgaca acatcgggtt gcattaccca tctatgtacc
    25081 aagacctgat caaaaaccat cgtttgactg aaatcgacta catcaatggt gccatttggc
    25141 gaaaaggaca aaaatatgat attgctacac catattgtgc ctttttgacc caattagtcc
    25201 atgcaaaaga aggaatcctt ggcgcagaat aaaaggctct ctgatgagaa tcagctgaat
    25261 cagcagctgg ttctcttttt ttaagcccaa taaaagatca aaaagaaaac acaactctca
    25321 tgatgccata agaattgtgc ttgtttgttt cagtacataa tctcttcaaa atgtaggttt
    25381 tttatttgtt ttcaacagag gagaggtcat attcgtattg ctcataaata gtggaatcgt
    25441 tagtgataaa tcgttttgcc caattttctg catcataaaa ttccaatagc tctgatagga
    25501 ttggcttaaa tggtgtatgc agaaattccg gtaattcatc taaatcacgt tctcgaagca
    25561 aaaaaatatt ttcaggacga taaaaatgat aatgagcaat cgcttgatta tccgtcacta
    25621 gtttcttttg aaaatacatg gattcaaatg tccgcaaagt cactccttgc tgaccctccc
    25681 gcaacagttc cagaatcccg cgcgattccc ctgttttttt caggtactct tcataagtaa
    25741 gatatccagg ttgttcattc cctcgatgtg gaagaatgaa atagttggtc gtaacatctc
    25801 tttttataaa ttcctgctta tagctttctg ctcgttcttt tctcccatta ttcgttgcac
    25861 caaaaaatag atccgtcttt ggttcgctag aagaaaaatt catatgtttc gaataaaatg
    25921 aactattatg tttcagccca agttcttttg cttccaaaaa atcaaaatga taaatctcat
    25981 cgattgccgg ttcatcttgt gcctgcttca gcatcgcctc gtgttctgct ttgaagtgat
    26041 tccaaaaaaa taacacaatt tttgcattgg taaatgggcg cacgcgtcga gccatctcca
    26101 tatcaaatcc ataatctaaa aaaacgatca gctgataatt tgccaactgt tgtctagctg
    26161 atttaatgaa ttccgactgt agtaaatctt ttccggacca aacatctaaa gagcggatgg
    26221 aaggcaaaaa ataaagtgat ttattttttg caactagcag tacgtcattt ttgttcatct
    26281 tttccgcctc actttctctg tcactttttc ctatgatagc ctgtttaaat agtaaaaagc
    26341 cagtaaaacg tttcctatat ttgtatgaaa atttaacatt tgtatgaaaa tatgatgaaa
    26401 taaatcattc ttctattaaa attcgcctaa aagttttctg aatagttgta attgtctgaa
    26461 aatttgttat cattagtcat tgttaaatat tgctatttat tcttaatata aattttgatt
    26521 gtgaatttaa aagaaagagg tcatttatgg aagataaaaa attgaaaaaa caacttagct
    26581 ccagacatat caccatgtta gctttaggtg gagcaatcgg tgcaggtcta tttaaaggaa
    26641 gtggcgaagc gattgggatc gccggccctt ctgtattgat tgcttttttg attggtgggc
    26701 tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt tttaagcggt ggcgacacgc
    26761 atcacggatt atctggtttg atacgccctt atctaggtgc tcattctgct gattttactg
    26821 attgggtcta ctggtctatg tggattatca atatcatcgc tgaagctgtt gcagctgctt
    26881 cctttttgca gttgtggttc ccgcacattc cagcctgggt atttgttttt atgttggctg
    26941 ttttaactac gatcatcaat ctttattcgg tacggttatt cgcagaaaca gaatattggc
    27001 tggcttttgc taaaatcagt gtcattatct tactgatcat ttttgctact tatcttgtcg
    27061 gtcagcaaat gttgggcaca ggcgttttcc ctacccttca gcaattgact gaccatggtg
    27121 ggtttactcc tcatggtatg aagggaatca tcagttcttt gcttgtagtt atctattctt
    27181 acggcggttc tgaactgatt gctatcacgg taagcgaagc agatgatcct aaacgtgcga
    27241 ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc cttttacatc attcctttgt
    27301 ttttgctttt gattcttttt ccttggaata ctttagcagg cacgaatgtc agtccttttg
    27361 tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga tatcgtcaat tttgtgattg
    27421 tccttgcttt gttctcatct atcaattcgg gtgtgtatgc atcttcaaga attttgttct
    27481 tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc tgttttaaac aagcatcaag
    27541 taccgcaacg tgctgtttta ttttgtacca gtgttcttta tcttggtgta gctctttctt
    27601 attttgtcgg tgacaaatta tttggctatc tggcaggttc cctttcgtat acagtattat
    27661 tgatctggat cattatcagt gcggcatcct ttgtactggc actaagaaaa ggaacattat
    27721 gggataaagg aatcagcttt tttgcattag ctgtcttagg attgattttc cttggtatcc
    27781 tgttcacgaa ctccattggg gtaactgtat tgactggcct gctttacctg ttcatttatt
    27841 tcagttatca aaaaaagaat gatgcgttcg tattgacgaa tgaatgaaat cgatagaaaa
    27901 ttttatttta tttatgtgga atagatggag ctattgaaga aaggagttgt gacaaaagtc
    27961 ttgttgcaac tcctttttcc gaataaacag tctcatgacc tcttggtaaa cggtgttcaa
    28021 aaaccctctg tgcaaaataa ttgattgttc tccatgctca tgacatgata gaagaaagga
    28081 atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga agtttttgtt gatgatcggt
    28141 gtttcaacgg tagttatgtt tggtatgatg tacttgaatg tctatgcgct cgatcatatc
    28201 ttcttcagtc gtacacgagt attcatggcg ttgatgatgg gtgcagtcat ggcagtcatc
    28261 atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga taaatgttat gatcttagcc
    28321 gctagtcttc ttgtttttgc cggttccttg tttatggtac gaagccaaac tactgtcaac
    28381 gacacagctt ggatgaaggc catgattcct catcattcta tcgctatcct ggcaagtaaa
    28441 cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg aagaaatcat tcaggcacaa
    28501 gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa tggaatgacc attatccgtc
    28561 caaataaaaa aagaggatgt tccagaagtt atcagcttca agaaataaga agaagctact
    28621 tctggaacgc cgtgaataag gggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    28681 gccaaacaaa aatagcttct cctatttttc gtttggttag gcttattacc gcagatgcaa
    28741 gcttttgaac accgtttatt aagaggttgt aaaatcagcg ttctgacctg agtattagag
    28801 agacaaatgg aaaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca
    28861 gctgattgaa caccgtttat cgagaggttg tgaaaaagtc gcttagtttc gcgtattaag
    28921 aaaaattttg tgaaatggct ttccacattt ttgtataaat ttggcttaat accgagaaac
    28981 tggcttttga ataccgttta ttcgtaaaag ggattgtgac aaaagtcttg tcacaatccc
    29041 tttttatgtt tttattgttc taattgcgct gtcttgctta ctgcttcttg cggtttcaca
    29101 aatttttttg agattcccca acctgtgaag gcactgacca tcgaaaacac aggagaaagc
    29161 aaactgaaaa atacgaatgg caagtaagtg agtgtaggaa taccaaatgt acttgctacg
    29221 aaacttccgg caattcccca aggcactaag tagttgatta ctgttccacc atcttctagc
    29281 gttctaccta atacagttgg atcaaatcct gctttttcat aagtttcttt aaatgcatta
    29341 ccaggcagaa taacagatag gaattgttca ccaataaaaa tatttactcc gatacttgtc
    29401 atcaatgttg taaaaatcaa atttggtgtg ttcgtcatct ttcttgatac tcgttccatc
    29461 acggttgaga tcaagccaaa ttcgatcaac aatccaccta atgacaaagt tagaataatc
    29521 aatgacacag taggcatcat actttcgatt cctccgcggc taagcagtga atcgatttgt
    29581 tggtttccag tatttgaaac aaaccctgat tcgatgaccc cagccaactt cgttaaggaa
    29641 gtttgtgggt tttgaataaa aatcattcct actgctacag cgatattcaa caaaatcgtt
    29701 aaaattgccg gcaccttttt ccatgcacac agtaacatta aaaataaagg aatcagtgac
    29761 cagattgaaa tagtaaagtt tgcttctaga acatcagtca cttcacgaat agatgaaaga
    29821 cttgcggctt tacttgtgtg tcctaaaatc atgaataaaa agaaagaaac aacaaatgcc
    29881 gggacagttg accacatcat atgcttgatg tgtttgaaca aatcggcatc aacaatagct
    29941 gctgctagat ttgttgattc ggataatgga gacatcttat ctccaaaaac ggcacctgaa
    30001 acaatagctc caacaaccag cgctggattt aagcctagtg ttgtcccaat accgaaaaat
    30061 gcaatcccaa tcgttgacat aacagtaaag gcactcccta cagcgctacc tacgattgca
    30121 cacacaacaa atacagaagg gacaaaccac tggacactga tcaatttgaa cccaatgacc
    30181 attagagttg gaatgatccc tgcttgtatc catacagcaa tcaatgaacc gactaaaata
    30241 aaaataaaga ttgggatgat tcctggtttg atgccattga tgattccttc gtgcagccaa
    30301 tctgtcgggt atctttttat cactgcatac aacatgacca ttccgatcgc tgctaagatc
    30361 gtgatattag gtgagacctt taatccgaca acgccaatcg caatgcctaa aacaataacc
    30421 attaatacaa gtgttgcttc taaaaaactt ggtgttcctt tttttcccat gataatctct
    30481 ccttttttag ttgttcttat gttctaaaaa agttaagata tcggattact ccgccgcaat
    30541 tcatccgcga ttcctccttt ataaaaataa aaagacctgt tttaaactaa ggacgaatga
    30601 ctccgcggta ccaccttaat taaaaacaga tctgtttttc actttcaaac ttttattcaa
    30661 atgtatggac tttccatgta attcgtcaat taggcttgat cggttcacag cgaccaccga
    30721 ctttctgaac aaaacaccta accgctactg cgtgaaaagt tgacttgtac gaagtttacc
    30781 ttccataaga tcatttgtca atgaaaaaga aaatattttt tcatttttct gaaaattatt
    30841 tttataactt tcacacacaa gtttattttt tactagaaag tagcgccctc ttgttttttt
    30901 cttgctacta tttcttgatg ttcttgcttg attctttcta atagctttgg ctctttcaac
    30961 agatcaagag ctgtattggc gagtagttct gctgcaatcg caatagaagc aaggcctttg
    31021 tcacttttag cagcagcttt gaattcttct gtgtgtccgg caatatatgt ctcagagatt
    31081 gctaccgtag gctggattgt tggtatgact tggctgacat ttcctacatc agaagaaccg
    31141 agacttgttc tttgttccgt ttctatttct tcatctggaa cacctgcttc ttttgcatgc
    31201 tggaagaata agtcatcaaa agaaggagta acgatgacat catcaactgc attttggaac
    31261 aatccaaact caaaagtcgt gcccgtactt atcgcagcac ctcgtacgat attttccact
    31321 ttttcatata ctttatctaa tgtttgccga tttcctgcac gtaaatagaa acgtccagca
    31381 gcgtaatctg gaatgacgtt ggcagctact cctccgtctg taatgatccc atggatattg
    31441 acatcttttg gcaattgcaa gcgtagagcg ttgattccat taaacacttg gatcaatgct
    31501 tccaaagcat ttactccttt ttcaggtgct gctgctgcat gggaagcgac accgtaaaat
    31561 ttgatgtcga ctggatcatt cgctaaagat tctgtcgttt tcccataacg aaaagctgga
    31621 tggacgcata acgctacatc gacatcctca aaaaaacctt ctcgaacaaa gctgccttta
    31681 gctgatccgt tctcgccccc ttcttcccct ggggtgccat atacacggat ttctccacca
    31741 gtttcatcaa tgaattgctt cactatgctg gcggctaata cggagtaagt accaaacaaa
    31801 ttatgcccgc atgcatgacc aatacctggt aaagcatcga attctgccaa aaaggcaatt
    31861 gtgggaccgg gtttattgga aatataacgt gcatcaaagg ctgttcggtg tccagctact
    31921 tctttttcta cccaaaacct ttctttttct aattgtttaa tcaacgctgc tgaggaataa
    31981 aactcatagt tacttacttc aggatgttcg tggatatcta gagcaagtgc ttgatattct
    32041 ggtaaacgat attggattag atccttcaat tggtttgttg agattgttgt tgtcataaaa
    32101 aattcctcct atgctagtca tttttcttat tttctcccga atgtttccca tgcaggcacg
    32161 cttgatcctt ttgatgtttt ctcgattact ttgactgtgt cttctgtttg ataagcgtct
    32221 actatttttt gatacgtttt attgttttca tcttcttttc ttgcaacgat gatattcaca
    32281 taaggacgtg catggtcatc cactggttct aagaagatgg catcagaagc aggattaaat
    32341 ccagcatcta cagctacacc gctattgatc actgaaatgt ctacatctga caaagcacgt
    32401 gctgtttggg aagcatctaa ttcagaaatg tctagatttt ttctattctc ggtaatatca
    32461 gaaacagtgg gcgcttgtcc ttttgcctta tccactttga tcaatccagc agtttgcaat
    32521 aacagaagag ctctgccgcc atttgtaaca tcattaggga ttgctacttt tgctccgtct
    32581 ttgatatcag ctacatcctt caaagtgtta gaataaattc ccagcggtgc attgacagta
    32641 tttccgatcg atactaaatc agttccgtgt tctttattgt aattgtctaa aaagaattga
    32701 tgttggaaag aattcaaatc aatcgatcca tctgctaaag ccacatttgg ttgattgtaa
    32761 tctgtaaatt tcacgtattc caaattaatg ttctcctttt tcaaacggtc tttcacgttg
    32821 tcccatacat ctgtatcttc tccaataatc cctagtttta cgatagtttc ttttttgtca
    32881 gttgtatctg cttttccttg gctacaccct gccaaccaaa taattgctac aaatgtaatg
    32941 actacaaaac gaataatttt tttcattttt tctcctccac tttcaatgtg taattttttt
    33001 aatcagttga ttactgataa attgacttaa aaaaactaag attagaatca ataatgtagc
    33061 cacaatcgtt acatctgttt ggaatcgatt ataccctctc gatatagcaa gattccctaa
    33121 tccgcctgca cctactgctc cagccatggt agtcagtccg atcacgttga tgatagtcaa
    33181 cgccgagacc cgaatgatac caggcaaacc ttctaacaaa tacacacgta aaatgatacc
    33241 gattggactg gttcccatcg cttctgccgc ttcgatcacc ccaggatcaa cttctagcag
    33301 tgcgttctcg atttgacgag caaaaaacgg aacaactcca aaaatcaatg gcactaaagc
    33361 tgctttcgca ccaatcgtcg ttccaactaa gaaacgagta gtcaatgcta aaagagaaag
    33421 caagataatg aaagggatgg aacgaatgat attgatcagc ttatcaagta tttcaaatag
    33481 cagacggtta gctagtaatc cattagggcg tgtcactact aacgtcacac ctaagatgat
    33541 tccaatcatc ccgccaatga atgctgtcca aaaagacata tatagtgttt gcttcgtcgc
    33601 ttcgacaaat tcatccggaa tcaaataaac attgggcaaa tatgttttaa agaaatcagt
    33661 cattttatct tcctccttcg atcactttga tacgactatc taaatcgttt ttttgtaaaa
    33721 tatttatttt tacaccagat ttgatcaaat aatccaatgc ttttttccgc tgtttggttt
    33781 ctccagctaa agtaacgatc aaactcccta acggcgtttc ttgtaataac tctacattgc
    33841 catataaaat attcgctgtt acttgatatt tgctgtacag atgagcgatt agaggttcgt
    33901 tcgtctgatt tccaatataa cttaattcca ccagccattt attggcgatc tgatcggcaa
    33961 aagcagaatg ggcaatgatc gtttctaatg cttggtcaag atgagtagct gttcgaataa
    34021 aatctttggt caattcttct tcaggattgc tgaagatctg gatgctgcta cctttttcaa
    34081 cgattcttcc atcttccata accgctacct tgttgcagat ttctttgaca acttgcatct
    34141 catgagtgat caggacgatt gttaaaccta actggcgatt caattttttt aacaatgcaa
    34201 ggatctgaag ggtcgttttc ggatcaagtg cactggttgc ttcatcacat aataatacct
    34261 taggttcatt cgccaaagct cgtgcgattg ctactcgttg tttttgtccc cccgacagtt
    34321 gtttaggata agcagatgct ttttcttcca aaccgacaag ttccagtagt tcattcactt
    34381 tttgacgacg ttcctgtttc gattttccag aatactttaa agaaaaatca acgttgtcaa
    34441 aaatagttcg gctttccatt agattgaaat gctgaaagat cataccgatg gcctttcttt
    34501 cttttcgcaa ttctttggga gagagctgac tcagattcgt ctgattgaca atgacttctc
    34561 ctgctgttgg tttctgcagc aaattcatca cacgaactaa cgtacttttc cctgcacctg
    34621 agtaaccgac gattccataa acatcgcctt tttcaatcgt taaatctaca gaatctactg
    34681 cctgtatctc tttttctttt tgtttgaaac atacagaaac atctcgcaat tcaatcatta
    34741 aaattcctcc ttatctaaaa aaagcgtcct caagaaaatg aaaatgtcat tttcttgagg
    34801 acgctttttt gcgtgttacc acctcatgtt caagctgtgt tcacacacaa ctcctcttca
    34861 agtacgatca tactctagca agatatcggt cgcagccgtc atcgccttac atctatgctc
    34921 gggatgaatt gctcgatggc cattttccaa tagtcgttcc ttgcttcttc tcagctaccg
    34981 aagctctctg tcaaaggttc ttctatcgta cttaccattt caatgcaaat aatattcaaa
    35041 aaaaaagtcc ttttactaac ataaactatg tcagtaaaag gacgacgtat cgcggtacca
    35101 ccttttttcg gaagtttcct tccctcaagc ccaacatctc aaaaagagag attggcgttc
    35161 gttttaacgg tcgaacagtt ccgaatttgc ctacttatcg acaaattatt ctccaaggcc
    35221 attttcagct tttcttactt gactcttctc accagccaga gcctctctgc aaagtttcca
    35281 aacttacttt ccttttcaac gaaatcgata ttttgttgtt gggattaatc tacatgagga
    35341 aaactgaaaa gtcaagtacg aaattcaaat tattttttct atatttattt ttctgttttt
    35401 ttatttaaca tttcaataac agttcgaaga tcttttcctt ccgcaagaag tttcaatttt
    35461 tccttttctt cgacggtata atttcggaca aaactggaat caggtatcaa aacaacgcct
    35521 aatcctgctc gtgaagcagc tgttgctcct actaaagaat cttccactgc aaaggctgaa
    35581 ctagcttcta atcctgttcg ttccaaagct gtcagataag gaatcggtga aggcttgttc
    35641 tccgacactt cgtctccgaa tgtaaacgtg tccaagtaat cagctaattt aaagtgatcc
    35701 aagatatctg ttgctctttt cgtaacagtg gtcgtagcaa tgcctagttt catatgacgt
    35761 tcttttaagt ctgccaatat ttcttttgca tatggtttta agtggatggc tccgcttgtc
    35821 agctgttgat aaaaatattc ttcacgcttc gctcggactt cctgtacttt ttcagggcta
    35881 cctgctgctt tcactaaaat atcaaaactt tgtttccagt tttttccgcc ccaagcgtca
    35941 acgatttctt ttgggatatg gatatcaaat tgctgaaatc cccaaagcca gccatcacga
    36001 tacgtttttt ctgtgtcaac taacaaacca tctaaatcaa atattgcacc tttcatctat
    36061 acattaatcc tttccaaaac aatacacata gtttagcacg actggtccaa aaagaacaga
    36121 agcaaagaac cagccttctc gtttataaga gttcttggaa tcgacggata tagattttct
    36181 tcacagctgt cacaagcgcc agataagcga tgactgtagc aaataaccat aaccagtaat
    36241 tgccaggaag tggcatcaag cctaattcag caccaaaact tgtaaaagga aggacagaac
    36301 cgacaataat tcctaatgta gtgataacaa acatggcaaa tgttgcgttg ctttggataa
    36361 atggtacttt cggtgtacgc agtgcatgga gcactaaggt ttgtgtccat agtgattcga
    36421 caaaccatcc tgcatggaat aatgcgatga aaatcactcg ctgtgaacta tccaatgtat
    36481 ggaaacttcc gcccactaca gcaggacaaa taacaaaata catcaacaag taagtagtaa
    36541 tatcaaatat cgaacttgtt ggaccaaagt aaaccatgaa ttccccaata ctagatgcat
    36601 tccatttttt tggctcgtcc aaatattctt tatccatccg atcccatggc aaggaaatgc
    36661 aggaaacatc atagatcaga ttcaaaaaca aaagttgtaa aggcagcatt ggtaaaaatg
    36721 gcaggaacgt acttgcaatc agcacggaaa acatattgcc aaaattagaa cttgctgtag
    36781 ccttgatata tttcatgata ttcccaaaag tttctcgtcc agaaagaagg cctctttcca
    36841 aaatcgtcaa atctttttct aaaaggatca cgtctgcaga ttctttcgca atatccacag
    36901 ctgtatctac agaaataccg atatcagcca ctttcattgc aggagcgtca ttgataccat
    36961 ctcctaaaaa tccgacagta tgaccatttt tacgtaaagc tgtcgtcaaa cgtgcttttt
    37021 gctgaggatt caattttacg aatacttcat ggtcttcagc tacttgtgct aattcatgat
    37081 cactcatttc cgagattttc tcaccggtaa ccaattcatt gatctctaga cctacttgac
    37141 ggcacactga acgtgtaact aattcgttgt ccccagtcag aacttttaca gctacaccat
    37201 gttctttcag agctttcaac gcttgtttcg ttgtttcttt tggtggatca aggaaagcaa
    37261 gataaccaat caaaaccatg tctgactcat ctttgactga aaattctcca acgacgcttg
    37321 gattggtctt ttgtgcgaca ccaatcactc gtaagccatc ttcattaagt gactgtactt
    37381 ttgcaagtac ggcttctttt cgttcttttg ttaatggtgt aacagtaccg ccaagatcaa
    37441 taaatttaga aatgttcagc atttcttcaa tcgcgccttt tgtgatcatt tgtgttttac
    37501 cagctggatc agcaaccaca acactcatac gacgacgttc aaaatcaaaa ggaatttcat
    37561 ccactttttg ataattgatc ttatctgtat ctaaagtttc tgttgcttca tcaatgatag
    37621 ccacatctaa caaatttttc agccctgttt gatagtagct atttagatac gcatggcgca
    37681 aaacacgatc atcttcattt ccttcacaat ccatatgata ttcaagaatg attttatctt
    37741 gtgtcaatgt ccccgtttta tccgtacata acacatcgat tgcaccaaag ttctggatgg
    37801 cattcaaatt tttgatcact gttccttttt tggccatcac acttgctcct ttgaccaaat
    37861 tcgtcgtaac gatcattgga agcatttcag gtgttagacc aactgctaca gatagtccaa
    37921 aaagaaaagc ttctagccaa tccccttttg taagcccatt gatgacgata actgctggag
    37981 ccatcaatga cataaatttg atcagtagca tggatgtttt atggatgcct aattcaaagc
    38041 ttgattgaat tggtttttca gatagcgttt ctgctacatg accaaatagt gtttcatttc
    38101 caacagaaac aatgattcct tccgcacttc cactgatgac attacttccc ataaagacaa
    38161 gattttcgta acttgtttcg ctttgctgat cgtcttcaca tactttcgca tgcttttcta
    38221 ctggatagct ttcccctgtc aatgctgctt gggaaatgaa taaatccttt gcggacaata
    38281 aacgcatatc cgctggtatc atatcccctg ctgaaagccg aacaagatct ccacagacga
    38341 tttcttccat aggaatctct gtgtactttc cattacgttt tacagctgcc gttactttca
    38401 caagtgattt cagtttttca gccgcttgat tggaacgtac ggactgaatc aacgtcattg
    38461 tcccgctgac gataaccata gtgaagataa tggctacacc gaaaagatct ttatctccag
    38521 gagctgctaa tacatattcc gtgatgaatg aaataattcc taatgcgatc aacactaatg
    38581 taaatggcgt gatgtatgct ttcaatattt ctacaatgaa tggtgttttt ttgccatatg
    38641 aaatcgcatt ttcaccgtaa ttttctcttg aacgttcgat ttgttcttct gtcaatccat
    38701 ttgctgatgc atggaatagt tcaaataatt cttgtttcgt ttttccagca aaagctgcat
    38761 agattttttg tgtatctttc tttttttcca tctcaatctc tcctttgtgt ccaaaagaca
    38821 gctgttctcg ctgcttttgg tctttagcta ttcatcggat cgatagagaa aaaactgaaa
    38881 ggggctgaga acagatagat aatagccctg gcctttcctc tagatccttg tttttcgaat
    38941 gactgtcatc catttgttct catctccttt actaagtaat tttaattcat gacttccaat
    39001 catttttcga atagttattt ttccaattag accacctcct ttttattttt caccgatggg
    39061 gatttgcggc aacaaaaaaa cctctcgcca aatactcagc gaaaggtttg aaaaagcata
    39121 ttaaaaaaag cttctcagat ctcatcgttg agttttgaca ctgtacaacg taaaaagcag
    39181 atacctgctt ttagctacct tatgaaaagc cttatctcga caattcctgt tctacccatt
    39241 ggcgtctctc gacgtttttg ggcagtagcc tatgtttgta taggagcctc acctaacaaa
    39301 tcttattcgg ttttccttcg ttaatataac aactaaaaaa gaggatgtca aatcaatttt
    39361 ccatgttttt catacttttt ttaaaataca cacgttatat tcctgtctca cgcggtttta
    39421 tttgtctcat tttccacctc ccgctataat agaaatatag taggaaacga ggtgctgtta
    39481 tggaaagaac acttgttatt atcaagccag acggcgtgag acgccattta gtcggaagca
    39541 tcatccaacg ttttgaagca aagggactgg ccattgctga aatgaagttt gaaactatga
    39601 ctcctgaatt agcaaaagaa cattatcagc acttgactga gcgcagtttt tttgatgaac
    39661 tgatcgatta catgacttcc ggtcctgtcg tttatcttgt gctggtcggc gaggaagtga
    39721 tcgatatcgt ccgtaagatg gttggagcaa caaaagctgc cgatgctgtt ccaggtacga
    39781 tccgaggaga ctacgcgctg ccggggactg aaaacatcat tcacgcttct gattcccgtg
    39841 atgctgcggt aaaagagatc gcccgctttt tcccgccatt cgatataaac aaaccgtctg
    39901 aaaaagcggc gaagtgatac aaattctaaa ctgctggtct agttgctggg ttcagcaatg
    39961 gccagcagtt ttttctctaa ggacctccgt tgattctttc ctaacatata ttcgagcact
    40021 gtgaagaatt cttggtagtc tttcgtcttt tcttccccta aagcagcaga aatcaatctt
    40081 tgataataat ctgccatcaa tgtttcatag atcactgtac ttctttcttt atgtgataag
    40141 ctccgtttca tatcttcatt cacactacgt gcctttttca accgaccatt ttctatataa
    40201 aacgaagtca ggttcattaa aaaagtaaag agatactgct catgattgtt caactgtgct
    40261 aatcgtcgca ttcttttttt gattttcaaa taattgttct caatgaaccg atcactgaat
    40321 atccaaggaa aatcagctag aatagatagt tcaaattgac catagtctgg tatctgcatc
    40381 aagtattctt caatgattcg gcattcataa cgtaaaaatt caaattctgt ctcagatact
    40441 tctgtttttt cgaccatgtg aagattcaaa accaaataat acagatcttc tgttttctta
    40501 aaatgcctac ggttattgct gatctcttta taaggatcaa agctgtcttc ttgtacccga
    40561 tctagcattc gtcttttttt ctttaacttt attggttcat ccagctggtt tcgcatccat
    40621 tcatattctg caagtgtcat gttcattcgc tctaaaaatg atctcaatgt tacaaatgac
    40681 atttttttca ctgaatttgt ctcaatcccg actagagaag tcttaaaaca gattccttcc
    40741 gctaattggg actgtgtcaa cttcttgttg actcgaagtt tcttgatcaa ttcaccatca
    40801 tacataatag ttctcctttt taatttaaat ttttccacat tcaataaata gatacgttaa
    40861 aaaggaaaaa tacacgaaaa aaataaaaaa atgttttttt cataaaaaat accaaccttc
    40921 aagcattaga atgttgtcta actactgaag gttggtttga ttgtttattg attatttttt
    40981 tgctgttcaa cgtctgttct tgttactgga tataagttgg ttgttgtttt attaccattt
    41041 tttgaaaaga cacttgtcga atgttcgcct ttttgctttt cgattgcttc attacgttcc
    41101 gcttcatttt tatagtcata ttttttcgga tcaatcgtct caaatccgtt tggcacataa
    41161 aaccgcaaga gattttcctg attcagctta tctgaaagac ttaacgcagt cttgactttt
    41221 tcctgatctt tggagatttc ctcatcgatt cctgctgctt tcgtatcgat gatttctcct
    41281 gtctgattat tgtaagcttt tcctcctagg acggtgtatt tgggtgtcac aaagttccca
    41341 tttcgaaaag caactacttg atcatgctga gaagataata agtctgtacc aaaatgaaga
    41401 taatctttat catcgattcc caatagatgt agtaatgtcg gcaaaacatc gatttctcca
    41461 ccgtattcat gattgactgt ccctttagta taaccaggca tatggatcat caatggcact
    41521 cgttgcatct gtgcgttgtc aaattcatcc catgtatctg gatctttacc aagagcgctt
    41581 gctaaatctt tattatctgt attcgagatc ccaaaatgat ccccgtaaat cacaaagatt
    41641 gaattttggt aaataccaga ttttttcaaa tacgtaaaaa attgttccaa tgattgatcc
    41701 agataatgtg ccgttcgaac ataattatct actgtgctat tgccagtatt caaagatgga
    41761 aagtcgaaat tcttgtcatc cgtataataa ggcgtgtggt tggttacgga aagaaatttc
    41821 gtgtaaaacg gctgttgtag atgctcaaga tattgggcag attctctgaa taaatcttta
    41881 tctaatatcc cataacctaa cgtttcgtcg gattcatcaa aataagatcg atcaaagaaa
    41941 ttgtcataac ctaagttttt atatacatga tctctattcc aaaaactagc tacattccca
    42001 tggaaaacag cgcttgtata gccttgcttt tgtccaagga tcgctggtgc ggcttgaaag
    42061 acattgtctg acccaagttc tgtgaataat gagccttgag gtaaaccaaa tgtgccagtt
    42121 tccaacatat tttcagcatc acttgtcttt ccctgtccta cttcatgaaa gaaattgtca
    42181 aagctgatcg ttgcttgatt ctggaaaata ctatttaaaa acggtgtgac ttcttgtccg
    42241 tctaccttca tattaatcaa aaactgctgg aaactttcta aatggagaac gatcacattt
    42301 ttccctttag cgatcccaaa tgtttcggga tttggctctg cataatgttt ctttgtataa
    42361 tctaaaactt catcaatccc attactgctt gcatgcgcgc gaacactgct agtcattcct
    42421 gttttgattc cgtcataaat agtatacgca tcgattccta gatatttgac aatatatgaa
    42481 cggtcaaatg ttcgctgcag caattgtgga cggttggctt cgctcagcgc tagatttcct
    42541 gaaaaaataa gccctgaaag acaggtaaca gcgatagcca tcggtttgcc gacagggttg
    42601 cttttgatag gtattttctt gaccttcaac caaaccagta gcccaatgaa cacagcgata
    42661 tccagccagt agatgacatc atggggcttc atcaaagaaa aggagcttcc tgacaatcct
    42721 tgcgacactt tcgaaaagcc taatacagat ttgactgtaa tgaaatctgt gaattcccga
    42781 taaaaaataa tattcaagta caacaaaagc gtatttaaaa tatcaatgat caaaagaata
    42841 gctaacgctg gctttggctt tttaatatac aaagaaaggc caaaaaacaa taaggtagtc
    42901 gcaattgggt tgatccacaa gataaagtac tggatcgtac cttccacacc taatgaaaaa
    42961 tctgcatagt aggcaatgat cgtcttcagc cagaaaaaga aaacagctgt caaaacgatt
    43021 gctaaacgat tttggcacaa ttttttaagt tgtgtcacgt atatcacttc tctctattca
    43081 cattttttct tattttgctc ctgaaaaatg aaaatttcct aaaaaagttg ttatcttaag
    43141 atttttttat tctttctaaa gattattccg tccacttttc acagcataaa accgctgcga
    43201 tgtaagcgat taaactaatg aagagataaa agtcttgtgg tgaccgcttc actccacggt
    43261 attcaaaagc cagtttctcg gtattcgtac aaaaaaagag gctgtgacaa aattcttgtc
    43321 gcagctcctt atttcttgat gcaggacagc tttttcacaa cttcttcctt aaacggtgtt
    43381 ctcctgccaa ctcctcggcc acaagtcaca atatttataa aacatgagaa gctgtttttt
    43441 gaaattgttc cttgtggctc agagttaaac ggcaggctca agacctcttg atacacggtg
    43501 ttcaatcagc tgaccctcgg tattagatta tgtcaggacg ctaattttac aacctcttgg
    43561 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga aaaatataag
    43621 gagctatttt ttgaaattgc tccttgtggc tcagagttaa acggcaggct caagacctct
    43681 tgatacacgg tgttcaatca gctgaccttc ggtattagct caaaaaacgg caaaatatga
    43741 ggaactattt tgccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc
    43801 tgtttttcat tgtccatagg attgatattg tccttgtgat gactcattcg taataattga
    43861 ggcttgattt tcttcacttc ccgttatcgt ttgtccactg ccagaatctg aaggacttgt
    43921 tcctgtcgac tcgttttttt gagaagagac agttgttttc ttgacttgtt catttgtctt
    43981 tttccattcc gctaaacgat cggcgaccac ttttttgtct actttttcag aataagcggt
    44041 gatcactttt ccatttttca aacgggctac tttgtcgcca tcatgagtca aaagcaaaac
    44101 ggtcaaatgc ttgtctttaa attgatattg tctgagagaa atcgcactaa gtacgtcatc
    44161 gatattaata aattgatttt cttcttttcc ttttggcaaa taaatgtcca attcgccttt
    44221 ttcgtaattg gcagttattt tatactccgc ccaccaataa gttgagaatt cttttttgaa
    44281 attatctact gattctttgc ttgctacgat tttttctgtt tctttctgag caagatctgc
    44341 tgtcattttt cctgaaaatg ctgcggataa aataagcagt aagaaaaaac cgaataacca
    44401 aaagagaaga aacggtcttt ttttcctcat gctttcctcc actcctattt aattttgact
    44461 attctattat atacaaaaaa tgaaatagaa agacaaaaaa gttgaaaaag atcgtaaata
    44521 attgaacttc tttttcacaa aatggtaaga atggagaatt tcacgcattt gctctcaact
    44581 attctttctt aagcctcaaa tccgttttta tctgatacgt ctttgaacca atgacccgat
    44641 tttttgattg ttctttttcc atcttgtgct aagtctactg aaataaagcc atatcgattc
    44701 ttatatgcat tggtccacga ccagttatcc atacatgtcc acatatgata gcctaaacaa
    44761 ttggcacctt cttgaatggc ttgatggaca tatttcagat gagattggac aaattcgatc
    44821 cgataatcat cgtggatcat gccatcttct ttgataaatc gtgcttcgtt ttctacaccc
    44881 ataccgtttt cagaaataaa gcagtcgata tttccataat gatctcgtac attgatcaaa
    44941 atatcataga ttccttgttc atagatctcc catccgcgat aaggattcat tttacgacca
    45001 ggcatttcat aattgtcgaa ataatcctca ggcattggac cgtttttagt atcgattggt
    45061 gtttcttttg cttttacccg gcgtggctgg taataattga tacctaaaag atctactgta
    45121 ttttctttga taaccgtcaa atcctctgca tggatcacag gcatgagatc aagttctttt
    45181 actatctcaa tcaattcact tggaaactcg ccttttacag ctggatcaag gaatgaacga
    45241 ttgaagaacg catctgctat ttgcgctgct ttaacatctt cagggtttga ttcgtctctt
    45301 ggatagcttg gtgtcagatt caagatgata ccaataacac caccttgctc catttcatgg
    45361 taagatttga tggctaacgc tgaagccaac gcttcatgat agccaacttg gatcgctttt
    45421 ttcatatctg actcttctgg ataatgccat cgatataaat aacctgcttc gactggaacg
    45481 attggttcat tatgagtaaa ccattttttt actttcgaac caaacaattc aaaacaactt
    45541 ttggcatatt tttcataggc ttcaaccgtt tctcggttca accatccgcc tttttcttgc
    45601 agatacatcg gcatatcgaa atgatacaga ttcacaaagg gttcgatacc gttttcaatc
    45661 aactcatcaa aataacgatg atagaaacga acggcttctt cgttgattgt tccagattca
    45721 tcaggtatca aacggctcca ttggatagat gttcgaaatg aattatgccc agtctgtttc
    45781 atcaactgta catcctcttg atacttcgta taaacttgcg aggttttttc aggtcctact
    45841 tgatcaaaga acttttgcgg ttcttttttg taccaataat caaaaatgtt ctctcctttt
    45901 ccatcgcctg aaatccttcc ttctgtttgt gggccacttg ctgcagaacc ccaccaaaaa
    45961 tttgctggaa attgatattt tttcatcaaa gcacctcttt ttctttttta gttcgtttta
    46021 gttgattcca tgtatgatat tttccatcac aatcactttt tcattttctt cctcttcttt
    46081 tttgattcgt tttaacagct gttctgctgc tcgttccccg attttatctg ttggtggcgt
    46141 gatcgtgatt ttcggaagga tactcagatc gtttttatct aaataatcat agtgactgaa
    46201 cgcaatatcc ttcccgattc tgacaccttc ttgattgaaa cattcgatgg caccaatcga
    46261 catattgtaa ttgctgacaa aaatagcagt tgcccctttt tctaataatg cttttgcccc
    46321 gtaatagccg ctttttctag aatagtctcc tgtaaagacc tctaattgga tcgatggatc
    46381 attttggacc gcttctttgg ctcctgctaa acgttctctt gctacgtagt ctgtttgagt
    46441 ggcagcaata aatccgattt ttttatgtcc ttgttcacca tgatgagcga tcacttttgc
    46501 tacactttct cgatcattca ctaatattgt atcaatacct gctggtcctt ctggacaatt
    46561 caatgcgata actggaatac tcatttttgt gagcatttca aatccagacc actcttcttc
    46621 ggataagaag acaatcagcc catcgatcac atgcttcatc aggtattcaa ttttttgttc
    46681 aattttttct ggctgatcgg caaacccgct taataataag ctatacccat tttcttcagc
    46741 tactctttcg atccctgaga caacttccga cccaaaacgg ctggaaatcc cgtttatcaa
    46801 aagtccaata gagaagcttc gattattctt caacccttta gcaatgatgt tttctttata
    46861 gtccaacgct gaaatcgctt ctgctatttt tttcatgttc ttttccttca gttgctgacc
    46921 attcaagtaa cgggaaactg ttcccactga aacaccagcc atcttagcca catcacggat
    46981 cgatgccata cattctcact cctgccagtt acatttgatt tctctttctt gttgttcgat
    47041 tatgatgacc gcattctttg catagtgttg cttgtcatca atatccatat aactaaagtc
    47101 ttcagtagag caacttcctc tcactattaa ttatacttaa acaagtattt gaaccggttc
    47161 atttttaata tagcattttt ttaaaagatt gcaacacttt tttaaaacgc tttctgaaag
    47221 tggcggattt tttgcctttt taaaatttta ttctaaaatt tactctattt gtgggtgttt
    47281 tgtgcgtttt tctcttccat ccgtttgaag ttttacattc caatggtata atttctttat
    47341 cctatcaaat gggggaagta ttttgaagaa attaatcgta ctatttattt tgtctgcggg
    47401 acttttgaca ttgactggct gtgatttgtt tggcggctct gaccaatcct cggacgaaac
    47461 aaccgattct tctgtcttaa aagcggaaaa agcagatacc accaacagtg tatatacgct
    47521 aaaagtacgt aaagatatgg atttatccgc gggacagttg acatttacag ataaaaaaat
    47581 ggaatggaca cgcacgtatc agacacaagg aactgattct tctgaatcat ctgaagaaaa
    47641 aacagtgctg acagatatca agatcactac caaagatgac gaatatatta tttcaggaaa
    47701 agaaaataaa aaagaaacaa cagtcatatt tacaaaaatc ggtaattacc gtttgaagga
    47761 tgaggatggc aacatctaca gtctatagtg ccgtctgttg gatcttcttt tgtttctccc
    47821 cttcttttct gctatagtaa agaagcaaga tgattgtaaa ggagacgaaa tggatgatcg
    47881 atagtcctga ttattgtaca tgtaataaca acaaaaatat ccatacagaa tttaatgacc
    47941 aaggaagctt gagcatatgc gacaactgtc acaaactagt caaagattca tttaaaacat
    48001 ttgaatctta tcctgttcaa aattatgtaa aaagtagaag aagataaaca aatccaagca
    48061 aaactcacta tggctgattt ttatgaactt caaactcagc ttcttccttt gatcaagctt
    48121 catcagtaaa acaaacagtg gatcatttca gtttacaaat caaagagtaa agatttattt
    48181 atttcaaata tttgaatata aaataaccaa acgatagaaa ggcttattct acatcacgta
    48241 gtaatttttc tttcccgtcg tttggttttt tatttttagc ctaattcttg accttttgca
    48301 tgctggttat ccgtaaaata ttttccagca ggctttcctt ccaattcagc taatttttct
    48361 tggatctgac ggatcacctt ttttgcatct tctggatttt ctacaaaatc gaatttatcc
    48421 ccatccactt gtattttagg acatacatca aaatcttcat accattgatt atagcgggtg
    48481 ttcaacgttt cataataggt atataacgtc tcatcgcttt ctagctgttc atagtcacgc
    48541 ccacgttttt tgatacgttc tagcatcgtg tccaaagaaa ctttgatatg gaccatcaaa
    48601 tcaggccgtt tttttggagc aacatcatct aactcgttta gcatcgtatc taataagcta
    48661 tcgtactgct gtacttcgat atccgttacc cgacctaaat cagcgtttag atggaacaac
    48721 aggctatctt catagatgga tcggtctagc acattgtcat cgttcactaa agcatttttc
    48781 attgctaaaa atcgcttgtt caaaaagaag atttgcaata aaaaagcgta ctgctcagga
    48841 tttgaataaa agagcggcaa tacttcgtta tcttcgacat tttcataaaa tggtttagaa
    48901 tcaatctctt ttgcaagcat atccgtcaaa ctgctcttac ctgcaccaat cgttcctgaa
    48961 atcaaaatca ctgacataca acccttcctc ttctataagt actcgcctac gtaagtttca
    49021 acaggcaatg tttcaaaatc atattcacgt acagcttggt ctgattttaa atacgtatgg
    49081 agactttgtg taatcatcaa gttcgtaggt accgtctctt cttgtacaat gatcatcggt
    49141 tttttcaaca tagttgccac acctaattcg taagccgttc ctggatcgat tgtttgatgc
    49201 tcaaagtcaa ggatagcaac aacaaattct gcatttgtca gttcttccat atctttttcg
    49261 tatacttcct gcgcccatcc agcgctgaat agttcataat tgctttcttg gtgatgacgt
    49321 ggagagtaga aacttgtgac tgttttgttt tcctccaacg ctttttcgat acgacttact
    49381 cgatcgattt gctcctctga aaaaaatggt cctgctaaat atatttgact cattattttg
    49441 cctccttcaa taataactga tttttaatag atactgctcg taattcattg accagttcca
    49501 atggattgtt ggaaaagatt acttgatcca accatttttt atccataaaa tcctcttttg
    49561 caaaagtttc taataattgt tgcaagccgc tgtaataacc gccaatattc aatattccga
    49621 ttggcttgtc gggcgtcaga ccgattgcta tccaagaaag aagttgtgag aattcctcca
    49681 atgtacctag acctccaggg aaaacgagga cagcatctcc cttttcaaat aataatacct
    49741 ttctttcatc gatcgtttct gtaggaataa atgtagtaac atcttttcca ggcaattcag
    49801 tttcaaaaag cccattcgga tagatacctg tgacttttcc gttattttcc agtactgttt
    49861 tactaatcac tcccataatc ccggagtatg aaccaccgta aaccagttcg atttcttctt
    49921 gtgccatgta ttttcctaac gtttgcgcaa taaatttgta actttcttta ttaccaaaac
    49981 gggaaccgca aaaaacagta agtttcataa aaaaacactc cctaactcaa aagtctatct
    50041 tcattctaca cgtgtttttt taaaataaaa ga
//
LOCUS       DAZAFF010000007        50001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000007 DAZAFF010000000
VERSION     DAZAFF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.007"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..1012)
                     /locus_tag="ACG9BB_000445"
     CDS             complement(2..1012)
                     /locus_tag="ACG9BB_000445"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN2836419.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1192..1536)
                     /locus_tag="ACG9BB_000446"
     CDS             complement(1192..1536)
                     /locus_tag="ACG9BB_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFN2836420.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1529..1963)
                     /locus_tag="ACG9BB_000447"
     CDS             complement(1529..1963)
                     /locus_tag="ACG9BB_000447"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFN2836421.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2101..2838
                     /locus_tag="ACG9BB_000448"
     CDS             2101..2838
                     /locus_tag="ACG9BB_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2836422.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2831..4042
                     /locus_tag="ACG9BB_000449"
     CDS             2831..4042
                     /locus_tag="ACG9BB_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2836423.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4246..5028
                     /locus_tag="ACG9BB_000450"
     CDS             4246..5028
                     /locus_tag="ACG9BB_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFN2836424.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5098..5760
                     /gene="trmB"
                     /locus_tag="ACG9BB_000451"
     CDS             5098..5760
                     /gene="trmB"
                     /locus_tag="ACG9BB_000451"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFN2836425.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5858..7612)
                     /gene="ade"
                     /locus_tag="ACG9BB_000452"
     CDS             complement(5858..7612)
                     /gene="ade"
                     /locus_tag="ACG9BB_000452"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFN2836426.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7744..8673
                     /gene="comGA"
                     /locus_tag="ACG9BB_000453"
     CDS             7744..8673
                     /gene="comGA"
                     /locus_tag="ACG9BB_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFN2836427.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8630..9679
                     /gene="comGB"
                     /locus_tag="ACG9BB_000454"
     CDS             8630..9679
                     /gene="comGB"
                     /locus_tag="ACG9BB_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFN2836428.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9676..9954
                     /gene="comGC"
                     /locus_tag="ACG9BB_000455"
     CDS             9676..9954
                     /gene="comGC"
                     /locus_tag="ACG9BB_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFN2836429.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9929..10399
                     /gene="comGD"
                     /locus_tag="ACG9BB_000456"
     CDS             9929..10399
                     /gene="comGD"
                     /locus_tag="ACG9BB_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFN2836430.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10371..10718
                     /locus_tag="ACG9BB_000457"
     CDS             10371..10718
                     /locus_tag="ACG9BB_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836431.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            10681..11106
                     /gene="comGF"
                     /locus_tag="ACG9BB_000458"
     CDS             10681..11106
                     /gene="comGF"
                     /locus_tag="ACG9BB_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFN2836432.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            11078..11437
                     /gene="comGG"
                     /locus_tag="ACG9BB_000459"
     CDS             11078..11437
                     /gene="comGG"
                     /locus_tag="ACG9BB_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFN2836433.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11609..12616
                     /locus_tag="ACG9BB_000460"
     CDS             11609..12616
                     /locus_tag="ACG9BB_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN2836434.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12641..13825
                     /locus_tag="ACG9BB_000461"
     CDS             12641..13825
                     /locus_tag="ACG9BB_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFN2836435.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14262..15845)
                     /gene="abc-f"
                     /locus_tag="ACG9BB_000462"
     CDS             complement(14262..15845)
                     /gene="abc-f"
                     /locus_tag="ACG9BB_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFN2836436.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15950..16078)
                     /locus_tag="ACG9BB_000463"
     CDS             complement(15950..16078)
                     /locus_tag="ACG9BB_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836437.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(16423..16566)
                     /locus_tag="ACG9BB_000464"
     CDS             complement(16423..16566)
                     /locus_tag="ACG9BB_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFN2836438.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16789..17268)
                     /locus_tag="ACG9BB_000465"
     CDS             complement(16789..17268)
                     /locus_tag="ACG9BB_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN2836439.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17341..17625)
                     /locus_tag="ACG9BB_000466"
     CDS             complement(17341..17625)
                     /locus_tag="ACG9BB_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836440.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17703..17897)
                     /gene="ssrS"
                     /locus_tag="ACG9BB_000467"
     ncRNA           complement(17703..17897)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG9BB_000467"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17969..19255)
                     /locus_tag="ACG9BB_000468"
     CDS             complement(17969..19255)
                     /locus_tag="ACG9BB_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFN2836441.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19393..20064
                     /locus_tag="ACG9BB_000469"
     CDS             19393..20064
                     /locus_tag="ACG9BB_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFN2836442.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20030..20518
                     /locus_tag="ACG9BB_000470"
     CDS             20030..20518
                     /locus_tag="ACG9BB_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFN2836443.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20531..21478
                     /gene="prmA"
                     /locus_tag="ACG9BB_000471"
     CDS             20531..21478
                     /gene="prmA"
                     /locus_tag="ACG9BB_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFN2836444.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21479..22243
                     /locus_tag="ACG9BB_000472"
     CDS             21479..22243
                     /locus_tag="ACG9BB_000472"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFN2836445.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22476..24689
                     /locus_tag="ACG9BB_000473"
     CDS             22476..24689
                     /locus_tag="ACG9BB_000473"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFN2836446.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24700..25146
                     /gene="dtd"
                     /locus_tag="ACG9BB_000474"
     CDS             24700..25146
                     /gene="dtd"
                     /locus_tag="ACG9BB_000474"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFN2836447.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25317..26576
                     /locus_tag="ACG9BB_000475"
     CDS             25317..26576
                     /locus_tag="ACG9BB_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFN2836448.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26665..27612)
                     /locus_tag="ACG9BB_000476"
     CDS             complement(26665..27612)
                     /locus_tag="ACG9BB_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN2836449.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28006..29298
                     /gene="hisS"
                     /locus_tag="ACG9BB_000477"
     CDS             28006..29298
                     /gene="hisS"
                     /locus_tag="ACG9BB_000477"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFN2836450.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29313..31082
                     /gene="aspS"
                     /locus_tag="ACG9BB_000478"
     CDS             29313..31082
                     /gene="aspS"
                     /locus_tag="ACG9BB_000478"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFN2836451.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31191..32066
                     /locus_tag="ACG9BB_000479"
     CDS             31191..32066
                     /locus_tag="ACG9BB_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN2836452.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32332..34215
                     /locus_tag="ACG9BB_000480"
     CDS             32332..34215
                     /locus_tag="ACG9BB_000480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN2836453.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34290..34574)
                     /locus_tag="ACG9BB_000481"
     CDS             complement(34290..34574)
                     /locus_tag="ACG9BB_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN2836454.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34564..35298)
                     /locus_tag="ACG9BB_000482"
     CDS             complement(34564..35298)
                     /locus_tag="ACG9BB_000482"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFN2836455.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35427..36050
                     /locus_tag="ACG9BB_000483"
     CDS             35427..36050
                     /locus_tag="ACG9BB_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFN2836456.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36242..37363
                     /locus_tag="ACG9BB_000484"
     CDS             36242..37363
                     /locus_tag="ACG9BB_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFN2836457.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37370..40498
                     /locus_tag="ACG9BB_000485"
     CDS             37370..40498
                     /locus_tag="ACG9BB_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2836458.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40517..43711
                     /locus_tag="ACG9BB_000486"
     CDS             40517..43711
                     /locus_tag="ACG9BB_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFN2836459.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43857..44564)
                     /locus_tag="ACG9BB_000487"
     CDS             complement(43857..44564)
                     /locus_tag="ACG9BB_000487"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN2836460.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            45329..45727
                     /gene="spxA"
                     /locus_tag="ACG9BB_000488"
     CDS             45329..45727
                     /gene="spxA"
                     /locus_tag="ACG9BB_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFN2836461.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            46059..46712
                     /locus_tag="ACG9BB_000489"
     CDS             46059..46712
                     /locus_tag="ACG9BB_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFN2836462.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            46938..47936
                     /locus_tag="ACG9BB_000490"
     CDS             46938..47936
                     /locus_tag="ACG9BB_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFN2836463.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            48112..49920
                     /gene="pepF"
                     /locus_tag="ACG9BB_000491"
     CDS             48112..49920
                     /gene="pepF"
                     /locus_tag="ACG9BB_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFN2836464.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        16179 a         8741 c        10453 g        14628 t
ORIGIN      
        1 tttattttga tgaatcagtt gtttcttcag ttgaagattc ttttgttgaa tcagttgatt
       61 ttgtgtcaga actctttgtt gaagctgtag tttcacttga gtctttcgta cttgatgaag
      121 ttgttgtgaa tgcactcaac acattttcaa aggcatcatc tttgattttt acatttgcat
      181 cttttaattc ttcaccgatt actttagttg tgaatgcatt gtcacttagt ttagtttcag
      241 tagcgatatc ttttagttga tctttgtatt tatccatgtc attgccttta ttttggtttt
      301 tcaccatttt gacaacgtag tattctgtcg cataagaagt aggatttgtt gtagtaataa
      361 catcggagat ctcaccgtct ttcaatttga aagctgcttc ttttacttct gcaggaatag
      421 ttgttgtcgt tgagtcgaat ttaactttac cgccatcttc ttttgtctct gtatcagttg
      481 atttgtcttt ggctaattta gagaaatcat ctccatcatc cgctgatttt ttcacatctt
      541 ttgcttcatc ttcacttgat aatttgatga tttgtgcttc tacttctgga tggaaagatt
      601 tccaagcagt tttcaaatcg tcatctgtaa tatctacgtg agctttcaag ccagcttcaa
      661 aagctaaatt gttacgaata tattctttat aagtatcttt cgtatatcca gcagcttcta
      721 attgtgattc aaaagtatca cctaatgatt tcgcttgttt gtcgtattct gcatccactt
      781 gcttatcggt tactttatcg ccatatttgt tattgaatac tttataaatg atcatccgtt
      841 gtactaagga ttggttcgat gattcaagtt ttgcttcatc atagaaatca gatacagtga
      901 tcgttccgcc tttcatcgtt gcgatatctt tattagtatc gcctgaacaa gctgctagag
      961 ttaaaacagc caacgcacta gtagctgcca aaataattga tttttttttc attaaaattt
     1021 gcactcctgt aattagttat tatttttata tattaactat accatatccc atttccttta
     1081 aaaacgttgt cgtggtagtt tcaaacaaaa ttcaaaattt catcacattc ctaggaaata
     1141 cgcaaacttt tctcacttat atagttaaat ctgttgagaa aataatcatt attatatttg
     1201 ttcaggatac tctggcagat cagcttttag tttttctact tgttcattga tttgtgtgat
     1261 acgtggttcg gcttggaatt taaaatcttc gatatctttt ttgattgctt cttggaaagc
     1321 aggaatcaca gattcagctg tttcttttgt ttcgatcaat gcgcttttaa aatgattcag
     1381 gctgttattt aaatcatttg tcagagttgt tgcttcttcc aattcttcga tcagtttctt
     1441 tcttgtttca ttcccgctcc tgggcgccaa taacagacct ccgacagtgc cagcagcagc
     1501 tccaaataac agtcctttga aaaagttttt aatcatcgca ggacacctgc tttacgattg
     1561 tttcttgaat agcttttagt tcttccgatc cataactatc ttgatgattg acaaagtgaa
     1621 tagaaaaatc atcctctttg ctgtagcgag ggactaaatg gatatgtgaa tgaaaaacag
     1681 attgataggc tagttcacgg ttgttattca agatattaag acctttcatt tctggaaaag
     1741 ctttttccaa tgcttgagca atcttaggca cacgcgaaaa aacctctcct gcaagttcgg
     1801 gatcatattc aaaaatatct gctacatgac gtttaggcac aactaatgtg tgtcctttcg
     1861 ttgcttgaga gatgtctaaa aaagcataga ccttgtcgtc ttcatatatt ttatagcttg
     1921 ggatttcttg attgatgatt ttgcaaaaaa tacagttttc catataaaac atcctttcta
     1981 taaagaataa atacttcagc ttaactttac cataagttaa agctattgta aggaaaaaaa
     2041 caaaataccg tcattttctt cgtggtataa taaataaaaa taaatggcgg aggaaaaaga
     2101 atgagtttaa ttatcgatca tgtcacaggg gggtacggac atctccctgt gttaaaagat
     2161 atcagtttcg atgtacagcc tggagagata gtagggttga ttggactgaa cggagcaggg
     2221 aaaagtacga ccatcaagca cattatcggt ctgctggaag cttcaaaagg aacgatagaa
     2281 atcaatcatc tatccttaaa gaaaaatacg gaagaatatc gcaaacaaat tgggtatatt
     2341 ccagagactc cttctttata tgaagaattg acattgagag aacatatcga agtcactgcg
     2401 atggcctacg atatacctat ggatatagca atgaaaagag cagaaaagtt gttggagact
     2461 tttcggttat ctaatcgtct tgactggttc ccagctaatt tttcaaaggg aatgaaacag
     2521 aaagtgatga tcttatgcgc ctttttgatc gagccttctt tatatatcat cgacgaacca
     2581 tttttaggct tggatccact agctatcaat gcattgcttg aattgatggt agaaatgaaa
     2641 aaagaaggag ccggtatttt gatgtctacg catattttag ctactgcaga aaaatattgt
     2701 gatcgttttg tggtcttaca taacggagag atacgagcag acggttctct tgagatgttg
     2761 agaaaagaat ttcagctgcc ggattcttct ttggacgaaa tctatattgc tttaacaaag
     2821 gagcaggcac atgagtagtt tttttcaaaa acgtttagca agacatcaaa agaaaatgat
     2881 gaaatatatg cgttatgtat tgaacgatca ttttgtttta gtttgtcttt tcttgattgg
     2941 cggtgtggga ttatattatt ctaattggtt gaaaaccctg cagccaccgt ttgcaattgg
     3001 agggctcata atctctgtat tttggatgct ctgtttattc gtgggtaaga tggctacttt
     3061 tgcagaggca gcagatattg tttttttact tcctaaagaa aaagaaatgc aaacctattt
     3121 ggaacgagct tttcgctatt cttgcatttt tccgttgatc tttttgatat tggcatgcgg
     3181 attggccatg ccattagtgg tggttagtac ggagcaatcc ttttcgatgt ttttcgtcta
     3241 tctgatcata ttatggagtt tgaaattcag tcatctgcaa atcaagagag cagaattgtt
     3301 tcgactagat caacaagtta ttcgttattg gaaattaggc tggttttttt cttctctgat
     3361 ctttttgttt ctatcatttt attggctaat atgggcagga atagcaggtg cattagtaca
     3421 ggcaagtcta tattactggt ttttgtggaa gcaacaagaa aaaggattgg attgggagaa
     3481 aatggtgcat gtagaagaac ataggttgca tcagatctat caatttatca atttattcac
     3541 agatgttcct gaaattacgg caaagatcaa aagaagacgc tatttggatc ctatattgac
     3601 tcatattaaa agagaatcaa aaaacactta tctttacttg tttgcaagaa gattggcaag
     3661 aggttctgat tttagtggtc tttatttccg attagtaatt ataggtgggt tattgattgc
     3721 cggagtagtc gacttttatt ttagtctgat tatcggtttc ttatttttat atttgatcgg
     3781 tttccaactg ttacctttag cgaaccagtt ccgctatatg gttctcgttc agctttatcc
     3841 aatccataag aatcagaaga agcaggcgat tcagtatcta ctcacatggg tattaattat
     3901 cgcgtcggtt atttttggtt tgattgctgc agtcgtatta gatgggcagc agcgatgggt
     3961 tccgctagcg gcttatttga ttgttactat gctctttgtt aaactatatg taccaactcg
     4021 tttgaagaag atgtcagact gaaaacaaag ctgtgtaggg taaaaatgag aatgaaaaat
     4081 agagcgttga cagtaagaat gctgttcgtt ctattttttt tgagaaaagc tggttttttg
     4141 aatggcaatt aaatgaaaaa gaacgtatat gtgcttgacg tgtagtgttc aggcgactag
     4201 aataataagt actgcaaaac aaagagtaag aggacgggag aatgcatgat ggagtttcag
     4261 ttagaccaag agtggcggtt acgtcccatc aaaggcgaca caggaaaaac atacataggc
     4321 atgcgtgatg aggacaaagt ctttattaaa cgaaacacga ctccgatgtt ggctgctctt
     4381 tcaagggaag gaattacacc aaaacttgtt tggacaaaac gaacggggaa tggcgatact
     4441 ttgacagcac aagagtggct ggaaggtcgc ttgctgactc ctaatgagat tggtcaaaga
     4501 aacgacgtga ttgatgtttt gtaccattta catcattcaa attctcttaa aaccatgctg
     4561 acccgtatcg gtggtcgtgt catgacgccg gagaagatgc tgcaaaaata tgaaaaagaa
     4621 ttaccggaag aactccagaa aaatcaattt ctgcaaattg tccatcgttt tcttcgtgag
     4681 cagatcccgt caatagataa ggaaaactta tcagtagtac acggagatgt aaaccatcgt
     4741 aattggttag tatgtaaaaa ttatttatat ctagtcgatt gggattcaat catgtttgca
     4801 gaccctgcgg tggatattgc aacgatttta ggaaattatg tgccactgtc tagctggaat
     4861 cagtggctga tcagttatgg catacgcccg actgacgaaa tgcttgaaaa atttcattgg
     4921 tatgcggcca tgaatttgct ccaagaaatc accagatatt gcaaacgtga agatcatcga
     4981 cggatgaacg aagaaatttt acaattaaaa cgaattttca gtggctgagg tttggcccaa
     5041 actctgcgga gatttggcca gaccttttta attagaggaa aggaatggag ataaataatg
     5101 cgtgtccgaa atcgtccggg agcagcggaa atgttagcgg ctcacccgaa ttttgtaatt
     5161 agtgatccaa cattgtggaa gggaaaatgg aatgaattgt ttgaaaacga tcatccaatc
     5221 cacattgaga ttggtatggg gaaaggccag ttcatcactg gaatggcaaa agcccatcct
     5281 gaaatcaatt atattggagt agaaatgcag gtcagtgttg tttcgattgc actagacaag
     5341 ttgatcgaac aaccgcttcc taacctgaaa cttctacatg tggatggatc agcgttgaca
     5401 gagtatttcg ctgacagcga agtagatcaa atctatctga atttttccga tccatggcca
     5461 aaaaaacgtc atgaaaaacg gcgattgaca tataagactt ttcttgctgt agatgaacag
     5521 attcttcgtc caaatggaga aatccatttc aaaacagata atcaaggatt gtttgagtat
     5581 tctctggcaa gtttttctca atatggcatg atcctcaaac aggtatggtt agatttacat
     5641 caaagtcaat ttgaaggcaa tattatgacg gagtatgaag aaaaattttc ttcaaaaggt
     5701 caacgtattt atcgagtaga agctcaattt caagataaga ccaaaaagaa aagtctgtga
     5761 aactaacttt ctgctttcat aaaaacatta atatccataa ataaatccca gaaaatgaac
     5821 attacagatg taatgtattt tctgggattt atttgattta aaatgtattt atttcatcgg
     5881 tttttccaca aggtggaatc aattggaagc gagaaaatga aaataatcct ttgtcggtga
     5941 tcttcaattc gggtatgaca gataatgtta agaaagaaag tgtcaaaaaa ggatcaaagg
     6001 ttttagctgc tcctagttga tgtgctttag ttattaattc attcaactgt tcatagacga
     6061 tcgaaaaagg ttcctgtgtc atgattccgc ctataggaag agaaacacaa gctatttcct
     6121 gctgattgtt gacaacggtc atcccgccac ctttttgaat caaaagatta gcagcataca
     6181 gcatatcctc gtcgtttgtc ccaacgacaa ctagattatg cgagtcatgg gcaatcgttg
     6241 tcgctatcgc tccttgtttc agctgaaatc ctttcactat gcctaatcca atgtttcccg
     6301 tttgatggtg acgctcgatc accgcaatct ttaattggtc tttttcgatt gaaggaatga
     6361 atcgttcgcc ttgtcggtcc actttttcta ccaaatcttt agtcactaga ctattcggga
     6421 taatttcgat cacatgggct tgtgaagaag aaagaagcag ctctaacgaa tgtggattta
     6481 atggttgagc gtttagtttt ggcagacaac acgtatatga atttgaatga tcagcaaata
     6541 aatggtcttg gagttgccca gattgaacaa ctaatgttcc cttatagaaa acttcatgaa
     6601 ttggcagctt ggtaagatcg tctgttaaga agaaatcagc ttgataccca ggggcaatcg
     6661 cacctagtgt tttcaaatca aaacattctg ctgcattcaa tgttgccatc tgaatggctt
     6721 gtaatggatc gattccaagt gcaatagctt gacggacgat atgatcgatt gatccttctt
     6781 ttactaaatc attgatcagt ttatcatcgg tgacaaagaa gcatcttctt gaattttcag
     6841 gtgtgatcgc cggtagtagc gatgccagat cttttgcaac ggtcccttct cgaaccatta
     6901 aatacatacc aagatccaaa cgttctttag cttcttttgc agttgtagct tcatgatccg
     6961 tccgaatacc tgctgcagga taaacattta actcttccat gccgatacca gctgcatgcc
     7021 cgtcaatttt tttcttatgc tggtgcatca attggatctt ttctaggatg tctgtttcat
     7081 tatttgctac tgcttgatag ttcatgactt cagctaagcc taaaacttcg ggtttttgta
     7141 aaaaaggggc taattttttt gcatttaaat ttgcaccatt ggtctcaaaa ggggttacag
     7201 gtacacaaga aggtagcata aaaaagatat tcatgagtgt ttgtcttgca tcttctatca
     7261 taaactcaag acctgtactc ccagcaatgt ttcctatttc atggggatct gtcactacag
     7321 tagtaactcc atgaagcaag gatacttttg aaaattcgct tggtgataaa agtgagcttt
     7381 cgatatgcac atggccatcg atgaatcctg gcactagata ttgcccatta gcattatgta
     7441 cgctttttcc ttcgtattca ccgattccta cgatcattcc ttcgcagatt gctacatctt
     7501 tttccatgat ttctttcgta aagacgttga cgatcttagc atgcttgatc actaggtcag
     7561 caggcttttt tttacttgct acttcgatat atttccttaa tgttgtttcc aatggagtgc
     7621 ctcccaagat aaatttttat aagtataaca taaattcggt ttttgccaat atttttttgt
     7681 aaattcattt tatttgaaac aaaaagatca aaaagacgta tgttttttgt ggaggggaga
     7741 ataatggaaa taaaacgatt atcggatcaa atgataagtg aaagttttgc caaacatgcc
     7801 caagacgtat atatattgcc agcttcagaa gtattttaca tctattttcg ctatggaaaa
     7861 aaacgaacat tctggaagaa agtctcttcg gcagatggac agcaactgat ctcaagattt
     7921 aagtatttgg gacagatgga tatcggagaa aaaagaaaag ctcagttagg tgcatttact
     7981 tattctttag aaaaacagga agttcgtctg cggttgtctt ctgtaggaga ttaccttcaa
     8041 cgagagagtt tagtgattcg gctattatac ggtacttttg aaacacccca tcgttgcttt
     8101 agttctcgag attttgcaac tattttacag aaaaccgtca gacgtggatt ataccttttc
     8161 agcggaccag tcggatcagg caagacttct ctaatgtatc ggttggcttt ggaagaaaaa
     8221 ttgcaagtga ttactatcga agatcctgta gagatagaag aaccgttttt tctgcagcta
     8281 cagatcaatc cgaaaatagc acagacttat gatacacttt tgagactcgc attacggcat
     8341 cgtccagact tgctaatcat tggcgagata agagatgaat caactgcaca tgctgcggta
     8401 agagcagcac taacagggca ccgggttttt gcaaccgtac atgcacgcaa tcttgaaggt
     8461 acattggtca gaatccatga actgacaaag aaaaaagaag agctatctga atgtttagct
     8521 ggtgtcattt atcaagaact tttggaagat gccaatcacc atccttctgt tttatggagt
     8581 tatcaattta tggaggaaga atcagtaaaa aaatcatggg aggaaagtta tgaagaagct
     8641 caggaaaata tttggcagtc agaactattc taaaggatta acaagaagcc agcaaaacca
     8701 gtttgcacaa accatgggag aactgctttt atctggtttt agtctgcaag aatcccttta
     8761 tttattactc acgatccgtt cgtttccacc tgttattttg gcaaatatcc aacatcagct
     8821 aaaggaaggg caagcgttta gtgaggtact ttcgcaaata ggatatggag cagagcaact
     8881 ggtacagatc gagttagctg aacgtcatgg gaatctgccg acaactttgt tggaaattgc
     8941 caaacagttt cggcttgttg aaggatttcg caatgatttg aaaaagctgc ttgcatatcc
     9001 gatgctattg ctcacctttt tgctaggtat cttgtttagt atgcggttgt tcgttttgcc
     9061 tcagcttatt ggttcaggaa tggtcatgca agagcattgg ggtattcagc tgatacaagt
     9121 ctttcattgg tatgttctac tattcatcct agcggttact ctattggctt tactagtaag
     9181 gtatcgtctg atgaagttgt ctcctgttga tcagagtgaa tggataagca gacaattatt
     9241 gattggaccg atatattcct tgtatcaatc ggcttatttg tcactagaga ttggaaagct
     9301 ttttcatgaa ggtttcgaat taaggcaaat catagtctgc ttaaaggaaa cgaaacaaga
     9361 aagtctgatt cagcatcttg cctctaagtt gattcaagga ttggaggcag gtatgtcatt
     9421 ggctgagcag ttccagaatt atcgttttct atcagaagaa ttcagtaaaa ttgttttaca
     9481 gggagaggca aagggaagtt taggtaaaga actgattttt tacagcgaat ggacaagaaa
     9541 acagcttttc aaacgtcttc atcactggat gcattttatt cagccaatca tttttttggg
     9601 cgtagcggct ttgattttat tagtttatgc cgctgtttta ttacctgttt acggaagcat
     9661 agaggaggtt ttgccatgaa aaagagaaaa atatcttatt atagcggttt tacattgatc
     9721 gaaatgctaa tcgtcttact gatcatcagt gtattagtgt tgttgttcgt ccctaatttg
     9781 tcgcgttata ggaatcatgt agaccaagaa agtcgtgaag cgatcatcca gttggtagac
     9841 acacaaaaag agctctatgc gcttcaaaat aatggaagag ttcctactgt ggaggaattg
     9901 ttgaatgaag gctatatcaa acgagaacat gcagaaatct atcagcggcc ttgaaggtaa
     9961 ggttttagca tcaggatata cgttgtttga atcactctta ctgattttat tacttacaac
    10021 gatcctttct tttccagttc tcgcattttc tgcatggaag aatgaattgg cagtctatca
    10081 atttttcggc caatttgaaa aacgaatcta ttctacgcaa aaaatcgcta ttgtgaatca
    10141 gatccaaaca ggcttttatt ggaatagtga agagaatcaa atcatttttc atgttccgaa
    10201 tccagataaa ataaattggg aagtattaga tattcctgat gagattacct tgaaacgcca
    10261 tgcttctatt acttttgctg ctagaaccgg gaatgaaagt tcactaaaag cttatcaatt
    10321 ttattgggaa gaaaaagagc ggacgattac gtatcaattc cagataggga gtggtcgcta
    10381 tacaaaaaga attgaataaa acaggatttg ttttgttgga gtcccttaca gcgcttatga
    10441 tcgcttctgt ctgtgtctac ttagtgagct ttggccagct gtttttgctt caggcagaaa
    10501 agaaaagtgg ggaggaactg ctatttattc gaatgattta tgaagatatc aagacgcagc
    10561 ggatgtatca caaaagacca gagcgaaggg aagaaatgat cccatacgat atccaaattg
    10621 tcgataataa tggagtaatc gaaaaagtgt ttgtcaatat ggagaaagaa gcgatcagta
    10681 ttgaaaaaac aagtaattcc agcatttacg ctgattgaat gtctaattgg actgatgata
    10741 ttgagcagtc tattgctcac attcaatctt ttgattcagc aaaccatcca agtaagtgaa
    10801 tttttgcagc gtaaagatca aaaagagtgg ctaatatttt tagcacaatt agaagaagaa
    10861 ctcaagagca gtcacaatgt ctctgtgaaa aatcagcaac tgcactacac gatcgaggat
    10921 aaacagtata tcatcgaaag atatcaggca atgatacgta aaagaagaac ttccggcgga
    10981 catcaaccta tgttaacaag tatcactgag cttcaattat tggaaaaaga acacacaatt
    11041 tctttttatg tacactttga aaatggagaa gatggatatg ccatatggac aaaaaacgat
    11101 cagtagaaaa tgttataagg gctctatttt attttctgtc ttgcttttat ttcttttatg
    11161 tacgttttta ttgatggcag cattagaaag ctaccgtttg tcaatggatc ttaatatacg
    11221 aatcaaaaat tattatatag caaaaataat gaagcagatg gctttatcgg aacagtctac
    11281 gcttgccgaa agttcggaag gagtttttta ctacacaact ggatctgtaa catatcagtg
    11341 ggtacaacag tccctattct tagaggtgga agtctcaccg tttatctttc ggttcacaga
    11401 agaagtaaaa aatgatacag atactagtac agaatgatgg aatgttcgtg aaaacgccct
    11461 cttttctgtt ataacactgt ttttaataaa aaaatgtttg ccgtttcaca aataaaaaac
    11521 agtgaaaacg attgaaattt tgtcaattgt tcgatagaat taaacaggtg aaaaaattgt
    11581 tataatatat ctagaaatga ggtgcaccat gttaccagaa aaaatggaac aagggtttaa
    11641 tcaaaacctg gaagctattc agcttttaca gaatgcattg ggaacttcct ttttagaagc
    11701 ctatgtcgaa aatgcagaaa atttaataga tgattatcaa gtacgtgtgg tggatggagt
    11761 gccgacaaaa gagacaacgc aacggatcac agctctttat gaagagttga agaaactttc
    11821 tttcgagcca gaagaatggc gcagactgtc tcaattactt ttattgaagg gcagccagac
    11881 agaacatttg caggcgaacc atcaactcac tcctgatagt atcggttttt tatttgtttt
    11941 tcttattgaa caactataca caaataaaaa agcaccggtt aagattctgg atattgcggc
    12001 aggaatgggc aacttgttat taacagttct tttaaaccta tcgaatgcgg ggtaccaaac
    12061 agaaggaatt ggtgtggata ttgatgatac attgttggct gtcgcagctt ctacaagtga
    12121 tttgacacaa gcaaatgttc aatactttca tcaagacggg ttgcaggaat tattaataga
    12181 tcctgtcgat tttgcaatca gcgatttgcc aatcggttat tatccgaatg acgaaaaagc
    12241 aaaagagttt ttaacaagta ccgaagaagg ccatagttat gcacatcatt tattgctaga
    12301 gcaatcaatg aaatatgtga agccggatgg ttttggtttg tttttgatgc cgagtggatt
    12361 tttagaaacg gatcaaagtg aagaaatcaa aaaatggttt aaagaagaag gttatcttca
    12421 aggaatgatt caattgccag atgaattgtt ccgtaataaa caatcgcaaa aaagtatttt
    12481 gatcttacag aaaaaaggac cacaagcaca acaagtaaaa gaagtcttgc ttgtgaaact
    12541 tgcttcatta aaagaaccag agaaggtgac ggaatttttc aacgagttta aaaactggaa
    12601 atcatctgct ctgtaatcat tcaacgagaa ggagagtcat atgtctaaaa caattgcaat
    12661 caatgctggg agttcaagtt tgaaatggca attatatcaa atgccagaag aaaccgtagt
    12721 tgcgaaaggg atcgtcgaac gtatcggttt acaagattct atttttacca tcaagtacgg
    12781 cgataatcaa aaattcgaac aaatcatgga tatcgaaaac cacgaagtag cagtaaaaat
    12841 gctgttagat cagttgattg aactgcaaat tttaagctct tatgatgaaa tcaccggagt
    12901 agggcaccgt gttgttcacg gaggagaaac ttatggggat tcagtagtga tcgatgagaa
    12961 agtaatggac cgcattgctg aattagcaga atttgctcct ttgcataatc ctgcaaactt
    13021 aatggggatc aaagcgttta aaaagatttt acctgacatt ttgagtgtgg ctgtattcga
    13081 tacatctttc cactcaacaa tgccaaagca aaattattta tatagcttac cggtgtctta
    13141 ttatgaagat ttcaaagtaa gaaaatatgg cttccatggc acaagtcatc gttatgtgtc
    13201 ggaacgtgcg gcaaaattat tgaacaaacc gatcgaagaa ctaaaaatca tcacttgcca
    13261 cttaggaaat ggtgtatcga tcacagcagt cgatggaggt aaatccatgg atacttctat
    13321 gggattcaca ccattagctg gggtgacaat gggtacacgt tcaggtgata tcgatccagc
    13381 tgttctccca tatttaatgg aaaaattaga gattgatatt gatgaaatga tcaatgtttt
    13441 gaacaagaaa tctggtctgc taggcctaac aggattgtct agtgatatgc gtgatcttga
    13501 aaagaactat gaaaaagaac atattcgttt agcttatgac attttcgtgg accgcatccg
    13561 aaaatacatc ggcggttatg tgacaacgat gaatggtgtt gatgcaattg tctttactgc
    13621 tggtatcggc gagaatgacg gacatgtacg tagtgaaatc atcaaaggta tgtcttggtt
    13681 tggttgtgaa atcgatccag cgttgaatga attgcgtggt gaagaagtag atatctctac
    13741 aaaagaatca aaagtaagag tattagtgat cccaacagat gaggaactaa tgattgcccg
    13801 tgatgtggag cgtctgcgcg gctaagccaa agcattcgtt aggataaaag atgaaacgca
    13861 tgcaagaggg aattcttgca tgtgtttttt ttgcggagaa gaccgcaaaa tggtaattta
    13921 acttcaattt cccttctttt ttacaggatc ttcttaggga attttgcttg gaaaagtggt
    13981 caaaagggaa aagactggca ttttatttat tgattttcag gaaaatccca atattttatt
    14041 ttcattcatc tttataaggg acattacata tgtaaaaaga aaaaacattt ttagatcagc
    14101 ctcgtgggct gtcgacttta ttcttcgctt tatgtttagc tagaaaaccg atccgaaaca
    14161 ttcattgatc gaataataaa aacttgattt tccacctgta ctttgcctga atcgtgaaag
    14221 cacaggtatg aaaatcaagt tttttgttgc gaaaaaacgt cctagtcaat cagttggccg
    14281 agcggctggc ggctgatctg ctcctgatca ataacgagtg cttcttggaa gtgttgctgc
    14341 aaaaagtagc ggtcatggct gactgctagg agcgttcctt gatagtcttg cagcatttct
    14401 tcaatttctt ctcgagcata gctgtcgagg tgattggttg gttcatccaa aatcaggacg
    14461 ttgatttttt ctaaaaagag tttcagcaac gccaaacgga ttttttcacc accggataaa
    14521 tctttgatcc gtttacccac atcgctttga taaaagccac ttttggctaa ttgccgtcga
    14581 gcgacttctt cgtttccagt caatgtgatg gcgtaagcca gcaaacggct atcgggatcg
    14641 gcaaactgca gctgctgcgg caaataaccg atttgcaagc tactacctaa tttgatggtg
    14701 ccaccgtaag gcttttcctc accgagtaat aatttgagta aggttgattt tcctgcacca
    14761 ttgtcgccga tgatggctaa atgatctcca cgatagactg caaaactgct gtctttgaat
    14821 agtacttgat cagcgtactg ctggtgcaag ttttgaatga tgaaaacttc tttgccagat
    14881 tttccaccat tagacaagct ttgcagccga tttttcgggg gaggtggcgg tttgacgagt
    14941 gtcagtttcg ccaggcggcg ttccagttct ttggcttttt tgaagaagga ctcattgtct
    15001 ccttcatgtg cccactgacg aaaacgacga atcatgactt tcaaccgctg gacttctttt
    15061 tgctgcagtt cgtaattttt tgtcagctca gccaagcgag cttctttcaa gactcgaaaa
    15121 cggctgtagt ttccgggata ctcaatcaat gcaccttctt ccagttccca tgtttttgtg
    15181 gtgacttgat cgagaaattg ccgatcatga gaaacgacca gaaccgattt ttttgtattc
    15241 tgtaaatagt tttccaacca gcgaatactg ttaagatcta aatgattggt cggttcatcg
    15301 agtaaaagta aatcggcatc ggctgctaaa attttggcta aagcgacttt aactcgttcg
    15361 cctccgctaa gttctgttaa ccgtgcttcc tgacggtgtg gaatccctaa gccttgcaag
    15421 atcgctgcaa tccggtcttc taatacgtaa ccgcctagtt cttcaaagcg atgctgcagc
    15481 tcaccgtaac gggccagaca cttctctaag tttgtatctg gttctgtcat caatgcttcg
    15541 tagcgactca tttgctgttt gaggtcctgt atctcagcgg cagaatggag caagtagtgg
    15601 ttgacggtcg cttcggaaac tgtaagttct tgttcgaccg tcccgatctt taagcctttt
    15661 ttgcgactga taacaccgct gtcgacacct tctctgccgg tcaagatttg taaaaaggtt
    15721 gatttgccag ttccgttgat accgactaag acaatttttt caccagcttc aatttgacag
    15781 gataaatcct caaataatgg attcccgccg attgtttttt taatagaaat tgcttgtatg
    15841 atcatgatct ctctccttga gaccactcaa aaaggccatg gttatccatg gcctgtgtta
    15901 tttaaactca gattatcatg agaaaaatac cttgagatgg ccattgattt tacgtgaatt
    15961 caactgtttt ggacaaactt gtcccgtcaa cagcaactgt aaaaccaatt gcatggctaa
    16021 atgcgatatg gtatcgttgg taattttcat tacttgttat tttagtcatg cgcatcatag
    16081 tattttccct ccttcgtgta gttgtctttt ccagtgtacc tgaaagtgtc agaaagttca
    16141 actagcgctg acatttactt aatatttatc gatgtgtaat tctgtcagag agcttttttt
    16201 atctgactga gggtaatgtc tcgtggacca catcattgta ttctgagcta aaccagcgtt
    16261 caggcgaaat catatggtgt ttgataaaaa actttgtcta catgacttcc ttttgccatt
    16321 tgcttaatac cgttgcatta aacagctctt ctctttggaa atctcaccaa catcgcgttc
    16381 aggagtatcg ttgtggttgc aatggcaagc ggtagccttt catcaattct tgaatgattt
    16441 accgaatgta gcacttgcct tctcgcggta cccgaacccg aaataaaatt ttagtttggc
    16501 gataaaatcg gtatccgcca tcgatacttc atattttttc gtatcagatt catcggaaac
    16561 ttccacattg acgaaccaat aagctttggc atgcttaggt cgttttaaac cacaccaggg
    16621 cgactaatcg ccctggtgtt cacttttttt tattttactt gaactcattg actgttttcc
    16681 ctttatttta aacattataa agaccgatga gtgcttgccg cgtcttttgt ttttatcacg
    16741 gttttttgtg caaaaaaacc tgccgatagt tggcaggtcg tctacgtgtt aagctttggt
    16801 gtggtcttta gctggcaatt ggttttctaa atcagttcgg acaacaagca catcgcaagc
    16861 tgcgtttcta ataacatatt cagaaacaga accaataaat aaacgttcta cagcattcaa
    16921 tcctgtggct cctaacataa tcaaatctac ttgattatct tctgggattt ctctggcaat
    16981 aatttgtttt ggtgagccat attctacgac agaggttaca ttattcaagc cagctttctt
    17041 cgcattgctt tcataatctg ccaatgtttg ctttgccatt tcagtagctt gttctgcaag
    17101 cattccatca aaggatgaaa cggtttggaa agaacgtgtg tcaatgacat gtgctaatag
    17161 aagttctccg ttatttcgga tagctacatt gaccgccttt ttaaaagcta attcagcttc
    17221 atcagaacca tctacggcaa ccatgatttt tttgtattgt tgtaacataa gcattcactc
    17281 ctcaagtcaa tcgtattgta ctcttttatt gtagaacact ttagggaaaa agagaattat
    17341 ttactgcttt ggttcagcaa ttttttttgc aaatcggata ctgaaggtgg cagaaacaat
    17401 aattacgatc agtaaaaaga ccaaggcaat aaagcgttga ttgaaaaaag ctgttaaaat
    17461 acccattacg cctaaaacag catatgcagc tccgtaaaat tgtaggaatc cttgattttt
    17521 atcatttttg ttgatcaata caaaaaagat atcctgttta ttaaataaat aatagctaac
    17581 gaataataat actaaagtgg cgataatcat taaaatccta atcatgaaca aatcctccaa
    17641 aataagcaac aaatagaaaa agcaatttgc caagagcaaa ttgctttatt ctttaaaaga
    17701 tggtaatctc cgttaatgcc gttgtttgtc ctgtagtatt gatcccgcga attcaagcag
    17761 gtgggtccca tggtcgtagc ttcgaactac caaatgataa ggcatgttac cagcagtgac
    17821 acaagacggg tcccaagata tcaataaaaa tgttcggtca acacacaaag aggacaattc
    17881 gaacatcaca gatttcccac ctctatagta gcatatgaat ttaaaaagag caatgagaga
    17941 gctcgcagaa acagaaccgt tttcttgtct agttttttgg aggatgcttt ttccactctt
    18001 ttatacggta atactgttga cccagagcct gttcatattt ccctgtatct tttggctgat
    18061 aatactgagc attcttcagt ttatctggta aatattgctg attgacccaa gcctgatcaa
    18121 aatgatgagg atactgataa cccacgccac ggttgagtga tttcgcacct ttgtaatggc
    18181 tatctctcaa atgatcagga acatctccag ctttgccctc tcgaatatca gcaagtgctg
    18241 catctaatgc catgtaggca gaattggatt ttggcgacaa gcaaagatcg acgacgacgt
    18301 ctgctagtgg gatacgtgcc tcaggcaatc ccaatttttc agcagctaat acagcgttca
    18361 ctgtacgagc cgcagcagca ggattaccta aacctatatc ttcataaccg ataaccatca
    18421 gacgccggca aatacttgcc agatcacctg cctcaactaa acgggcgaga taatgaagtg
    18481 cagcatccac atcacttcca cgaatcgatt tttgaaaagc cgaaataacg tcataatgag
    18541 catctccatt tttgtcatgg gtaagagctt ttctttggac acattcttca atgatcgaca
    18601 gtgtaagatg aatccttcct tctttatctg aaggagtcga tagtgtagcc agttccagcc
    18661 cattcaatgc actccgcaaa tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt
    18721 cgtctaactg gattgcttgt tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga
    18781 ctgccaactg gatgtcttgt tctgttaatg gtttgacttc aaagatctgt gtacggctgc
    18841 ggatcgctgg attgatggta atataaggat tctcagtagt cgctccgatc aagatgattc
    18901 ggccactttc taaatgagga agcagaaaat cctgttttgt tttatctaat cggtgtactt
    18961 catccaataa gaggatcact gtaccactca tttttgcttc ttccgctaca acttgtagat
    19021 ccttttttga atctgtagcg gcgtttagca tgcgaaatgc ataattagta gagccggcga
    19081 tcgcacttgc gatactcgtt tttccagtac caggaggtcc atatagaatc atggaagaga
    19141 gcatgcgagc atcgaccatg cggcgtatga tttttccttc cccgactaga tgctgctgac
    19201 caacgacttc atcgatcgtt cgaggtctca tgcgataagc caatggctgt tgcataggat
    19261 tttcctcctt cttgttttta cgtcccttaa ttatatcata tttagcaaga tgcctagaaa
    19321 gaacagcctt ctgttgtttt gttttcatag cttatccaac taatattgtg tatgataaag
    19381 taggtgagaa aaatgacgga taaacaaaag atagatgaat ttttttggaa taaaacgacg
    19441 ccagaagtgg cacgggattt attggggatg tatttggagc atgacacacc agaaggaaga
    19501 ttagctggat atattgtaga cgcggaagca tacctaggtc cagaagacga ggccgcccat
    19561 agctatggtt taagaagaac accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc
    19621 tacctttaca cgatgcatac tcatcggatt ttgaacatca ttactcagcc agaagggatt
    19681 cctcaaggag taatgatacg tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa
    19741 aatcgtggcg gaaaaactgg tccagatatc agcaacggtc cgggaaaatt agtggaagca
    19801 ttagcaattc ctcaagaact ttatggtcag tctatagcag atagttcgtt acgcttagtt
    19861 tttgagaaaa aaaagacacc aaaaaagatc aatgctttgc ctagaatcgg tatcccaaac
    19921 aaaggtgtat ggacagaaaa gccgttgcgt tttgttgtgt cgggaaatcc ttatatctct
    19981 ttacaaagaa aaaaccaaat cgagaaagat tggggttgga ggaaagaaaa tgaaaaagaa
    20041 ggatctatta acatatttag atgaaataat tgaaaaacaa gtgacagact atgatgtagc
    20101 tttagattgg gatacaaaaa atcatacgat cgagatcgtt gttcgcttgt ttgcagaaaa
    20161 taaagcacaa tttgagattg atgatgcaga agggatcgtt tcgcaagaac caattatcga
    20221 atttgaagac ggcgtacttc tttttaatcc tcagaaatct gtttttgatg aacgcaatta
    20281 tctagcagta atcccttatg aaggaaaaaa agggttggca aaatcagtag ctgattcttt
    20341 agtggaatat ctaaatgaag tattagctca agggcaaagc gatttgcttg attttttgga
    20401 tgaagacgat gacgaagctg tattcgagct tcactgggat aatcagcaac tagcagaatt
    20461 agtagaagta aaacagcaaa atgaagcaga tacttattta ccgtacccaa gttattaagg
    20521 agtgacagat atgaaatgga atgaagtaaa agtagaaact gaaagtgaag cagtcgaagc
    20581 agttgctaat attttgatgg aggcaggggc gagtggtgtc gcgattgaag attctttaga
    20641 tgttgaacat tttcagtcgg atcaatatgg tgagatttta gataaaaatg attttacatc
    20701 gctgaaagaa ggcgcactag tcatggctta ttttccggaa acgactttct taccggaaat
    20761 cttgcctttt atcaaagatc gtatcacaca attacctgag tttggtctag ctattggaaa
    20821 aaataaagta acagttagtg aagtagagga aagcaactgg gcaacagcct ggaagaaata
    20881 ctatcatccg gtgcgtatta cgcgaatgct gacggttgcg ccaagctggg aaaactacca
    20941 gacagaagat cctctagaaa aaattatcac actagatcct ggtatggcat ttggcacagg
    21001 cactcatccc acgacttcct tgacattgac agcactagaa acagtcttgc gtggtggtga
    21061 aacattgcta gatgtgggaa ctgggtcggg cgtgctaagt attgcagcaa aatatcttgg
    21121 tgcaaaagat gtctatgcct ttgatttaga cgaagtagcc gttcgttcgg cgaaagaaaa
    21181 tatggatatg aatgaaatag ccaaagatgt ccatgtatca gctaacgatc ttttgacagg
    21241 aatcgagatt gaaagtgatg taatcgttgc gaacatttta gcagatatca tcttattgat
    21301 gatcccagat gcttggcgtt tactgaagca aacaggcacg ctgatcgttt caggaatcat
    21361 tgaagagaag aagcaaatgg ttctggatgc catgttagaa caagggttta tcgtggatca
    21421 aattttccag caaaaagact ggtatgcaat tattttgaaa aaaccagagg tggaatagat
    21481 gcagcggtat tttttaaaag aatcatatga atcaaaagac tattttgttt tagaagggga
    21541 aaactatcac cacattgtac gggttatgag gatggaaccc gaggatgaag tttttttggc
    21601 attcaatgat caaatagcca ttgttgcaga aatcacggag attactgaag aggctgttta
    21661 tctgaaagaa attaaaaaag aagaaaatca aaaggaactg ccaagttcga ttaccatagc
    21721 gagtggctat cctaaagggg aaaagctgga ttggatcgtt cagaaatcca ctgaattagg
    21781 agcgcaagct tttcttggtt tcccggcatc agcctctgtc gttaaatggg atgcaaaaaa
    21841 acgtatgaaa cgacaacaac ggcttgaaaa gattgctcaa gaggcagcgg aacaatccca
    21901 tcggcagatc gtgccatcag tagagttatt ggaaacgaaa caagatttat ttgagcggct
    21961 agatgattat gacgtggtct tggttgctta tgaggaatca gcaaagcaag gcgaaaaatc
    22021 aaatgcagcc aaaattctta gtgaaacaaa accagatagt cgaatcttag ctgtctttgg
    22081 accagaaggt ggattatccc gcgatgaagt cgattatttt gtagaacaca aagggatact
    22141 ttgtggatta ggaccaagaa tccttcgtac agaaacagca cctctttatt tattaagtgc
    22201 tgctagctat caatgggaat taatgaatcc ttctttcagc taacgttctt cattaataaa
    22261 agagtatcga cagaaagaat tgaaaaaata gtcaacaatt tgtataatag aaacaattat
    22321 ttatacaatg gatacgaaaa atcgtttgaa aaagctttta aaaagcgaac atttccgtaa
    22381 aaaaacgtat aactgcacgg attttgggac aattatcaaa cagatatttg tcccgattcc
    22441 cgttttaccg aaaaaagaaa gggggatcga ttcctgtgac aaaagaagaa attttaacgg
    22501 gacccggtgt cataaagctc gttagtcaat acatgggtcc cgaacatgta gcatttgttg
    22561 agaaagcatg cgaatacgcg actgctgcac atgacggaca gtttagaaaa tcaggcgaac
    22621 cctacattat tcatcctatc caagtcgcag gtatattagc agatttaaaa atggatcccc
    22681 atacagtggc tacaggcttc ttacatgatg ttgttgaaga tacagaaatc actttagaag
    22741 atctgagaga agaatttggc gatgacgttg ctatgttagt ggacggcgta accaaattag
    22801 ggaaaataaa atataaatcc cacgaagagc agctagcaga aaaccaccga aagatgctgc
    22861 tagcaatggc tcaagattta cgagttatca tggtcaaatt agctgataga ctacataaca
    22921 tgcgtacgtt gaagcacctg cgagaagata agcaaagaag gatcgctcag gaaactttag
    22981 aaatctatgc gcctcttgca catcgtctag ggatcagccg gatcaaatgg gaattagaag
    23041 atacagcact tcgttatcta aatccaaaac agtattaccg tatcgtccac ttgatgcaga
    23101 cgaagagaga agaacgtgaa aaatacgtaa gtggtactgt tgaagatatt cgaatagcga
    23161 cagaagagct ggggattttt gcagaaatct atggacggcc aaaacacatt tattcaatct
    23221 atcgtaaaat gaaagatcag aaaaagcagt tcaacgaaat ttatgacttg ctagctattc
    23281 gagtgatcgt agattcaatc aaggattgtt atgctgtatt aggagcaatc cacacaaaat
    23341 ggaaaccaat gcccggcaga tttaaggatt atattgccat gccaaaagct aatatgtacc
    23401 aatctttgca tactaccgtg atcggtccgg caggaaatcc ggtagaaatt caaatccgaa
    23461 cacaggaaat gcatgaaatc gctgaattcg gggttgctgc acactgggcc tataaagaag
    23521 gaaaaaatga aaaagtagaa ccagatggta tgacgaaaca attaagctgg ttccatgaga
    23581 tactcgaact tcaagacgaa agctatgatg cttctgaatt tatggaaggc gtaaaaggag
    23641 atatctttag tgataaagtc tacgtcttca caccaaaagg agacgttact gagttaccaa
    23701 aaggatccgg accattggac tttgcataca gtatccatac agatatcggt aacaaaacca
    23761 ctggtgcaaa agtaaatggc aaaatggtgc agcttgatta caaattgaaa aacggagata
    23821 tcattgagat catgacttct ccaaattcat ttggcccaag tcgcgactgg ttgaaattag
    23881 ttgctactag caaagcaaga aataagatca aacgtttctt caaagcccaa gatcgagaag
    23941 aaaatgtgat caaaggccac gaatccgtgg tcaaatgtat tacagatcta ggatttacgc
    24001 ctaaagatat tttgacgaag aacaaactgc aagaagcact cgatcgtttt aattatcaaa
    24061 cagaagatga tctctatgca gctgtagggt atggagaagt tagccccttg acgatggcca
    24121 atcgtctgac tgaaaaagaa cgtaaagaac aaaaaatcga gcagcaaaag caagaagcag
    24181 aagaaatcat gaatcagccg aaaaaagaac ctgacaaaat gaaagtacgt catgaaggtg
    24241 gcgttgtcat tcaaggggta gaaaacttac taattcgtat cagtcgctgc tgtaatccga
    24301 ttcctggtga tgatatcgtt ggttatatca ctaaaggcag agggatatcc attcatcgtc
    24361 gagattgtcc gaatgttcag cctgacaaac caaatgtagc agaacgtttg attgaagtcg
    24421 aatgggaaga tacatcgaat acacgaaaag agtatgatgc agatttggaa atttacggct
    24481 ataatcgttc aggcttattg aatgatgtac ttcaaacagt caatgcgcta acgaaaaatc
    24541 tcaacagcgt tgaagcacgg acgaataaag ataaaatggc gacgatccat ttgacggttg
    24601 gtatccagaa tttatcccac ctaaagagta tcgtggataa aatcaaagca gtacctgatg
    24661 tctacagtgt acgccggacg aatggatagg aggacagata tgagagcagt tgttcaacgg
    24721 gtaagtaaag ctgctgtgtc agtcgatcag caagaaattg gaaaaattgg ctacggccta
    24781 cttgttttat taggcattca tgaaaccgat acgcaaaaag atgtggacta tctggtaaaa
    24841 aaaattgcac aaatgcgtat ttttgaagat gaacaaggaa aaatgaatat tagtgtagaa
    24901 gatgtgggag gacaaatttt aagtatctct caattcacac tgtttgcgga tacgaaaaaa
    24961 ggaaatcgac caagctttgt ggaagccgca cgaccagaaa cagctattcc tttatacgaa
    25021 tcattcaatg aaggaatcag aagtcgcggc atcacagtcg ctaccggaca attcggagca
    25081 gatatgtcgg tttctctcgt caatgatgga cctgtaacga tcattattga ttctaaaaac
    25141 aaataaagta atctggtttt cactagtaaa aactatgaaa cacgtcgctt aagttagact
    25201 gataaactct aacttaagtg gcttttttgt ctgcaaaaag cgcttctttt gtcagcagtc
    25261 ggaaaaaaag ttaagcttaa attatacaag ttatttttta taggaggttg ctgaagatga
    25321 aagaagtacc agtagtagat atcaaaatca cagatttaaa aaaattgtat caaaaaggat
    25381 acaacatgtt ggaggagtta cgccatgaag cggacgctcc cgtcgtcaaa gcaaagattt
    25441 tcaataaaga agctatcacg atctacggat catcagcagc aaaagtattt tatgatccaa
    25501 ggaatttcaa acgaaaaggt gctatgccaa aattagtatt gaagacactt tttggtcaag
    25561 gtggagtaca gactttggat ggagctgctc atcaccatcg gaaaaatatt tttatggatc
    25621 taatgacacc ggaaagaatg gaagattatc atcgtatctt agataagaat ctgacacaag
    25681 cacttgaagc acagcatggt caatttgaac tatttgattt aagtaagatg gttttcttta
    25741 catctatttg cgagtgggca ggtatcaatt tatcagcgat ttctaaagat gaggtcgaaa
    25801 aattagcaga atatcaaatc tctatgatca gcggaacgtt tacttctccg attgatcaca
    25861 taaaaggtgt cgaaaatcgc aaaaaatcag aaaaatgggc gcaaggcttg attgaagaag
    25921 ctcgacaaaa tcctgtggct ggaaaagaaa atgtggcatt atatgctttt gccaatgcca
    25981 cagatttaga tggtcagctt ttacctctgg aagtggcagc tgttgaatta ttgaacatca
    26041 tccgtccaac ggttgctcta actgtgtggg cggcattgat gggacatgca cttttcagta
    26101 gaccagattt gtatcagcaa ttaaaaaatg acttttcgac gttacaagat ccgtttattc
    26161 aagaaatgag acgctattat cctttcttcc caatgctacc tgcgatttcg ctgaaagaag
    26221 tagaagtaga tggttatcgt ataccagaag gcagttgggt tattttagat ttatatggaa
    26281 cagaccatga tgaacgaaca gttgaagcgc cagattcctt tatgatcaaa cggtatgtag
    26341 ggaaagcaaa agatatttcc tataaagaag aatatgagat gattgcacaa ggcgggggaa
    26401 attttcgaca aatgcatcgc tgtgcaggag aatggatcac tcttcatagt ctacgtgtgt
    26461 tttccgatca gctagtaaat aagtttgagt tctctgtacc tgaacaagat tggacgattc
    26521 cattcaatca gtttccgact tatccgaata gccgagcgct actttacaag aattagatta
    26581 gcataaactt atcgaaaaaa aggaagatca aacagtgctg atcttccttt tttcatatta
    26641 ctataaattg ataaggtcaa ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata
    26701 atcttcattg gataaatgca aatctaaagc ttggacattg tcagatactt gctctggacg
    26761 cttagctcca ggtataacta cagacactcg tggattcttc atgtaccaag ctaaaacaag
    26821 ttgcgcagga gtggcttggt atcttttagc aatggggcga agtacatcga cagcggttaa
    26881 aatttctctg aaacgttctc cttggaaatc tggatttctt tttcgaggat ctccttcacc
    26941 aaattgtttt tcctcaccaa gttcatattt tcctgtcagc aaaccggaag caagaggaaa
    27001 atagggaaca aaactgattt tgtttttttc aagataaggg aataattctt tttcagcctg
    27061 ccgatggatc aaactgtatt tatcttctac tacatcgaca tatccatctg cattcgcttc
    27121 tttcaattgt tctaacgtga agtttgacac accgactgct cggatttttc ctgcttcttt
    27181 taattcgtgc aatgtagcaa ctgattcatt ttttggtgtg ctttcatccg gaaagtggat
    27241 ataaaaaatg tcgatgtaat cagtttgcag acgttttagt gcatcttcca ccgattgttt
    27301 gagaaactca ggagaattat caaatgttcg ccctttattt ggtacatgag cagcttttgt
    27361 agcgatgacg acacgtgagc gatcatattc tttttccttc agtacctcgc caatcagttc
    27421 ttcggaacgg ccgtttccgt aagcataagc ggtatcgatc aaattgatcc cgctattcaa
    27481 agctgtacga actacttgtt ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc
    27541 tccaacggca tttgctccta atccaagctg ttctgcgtag acttgtgaat gtccgattct
    27601 tatttctgcc atagctgatt cctccttttt gtctagtcta aaacaaaata ccaaaaagct
    27661 gaactaaaac gtttttgtga ggggaaatag tcagatatct cttgactttg agactggttt
    27721 ttagtaggtt ataagtaata tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt
    27781 acaagagagg atcatcgagg ctgaaaatga tctaaagttt cattagccta aagacactct
    27841 ggagatggac gagtgaaaag ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa
    27901 atctttcaaa attaggtggt accgtgtatg aatttcattc acccttgttc atgatgaaca
    27961 agggtgattt ttttatttac atagctaaga aagggagaga aaactatgag tttccaaaga
    28021 ccaaaaggaa caaatgatat tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa
    28081 acagcacgtt tattattcag agattatcag tataatgaaa tgcgtacacc gatttttgaa
    28141 cactatgaag tgatatcccg aagtgtaggg gatacaacgg atatcgtttc aaaagagatg
    28201 tatgattttt atgacaaagg ggaacgccat gtgacgcttc gaccagaagg tacagcacca
    28261 atcgttcgtt cttttgttga gcataaattg ttcggcccag agtatgcgaa accgttcaaa
    28321 actttttata tgggaccaat gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa
    28381 ttccatcaaa tcggagtaga agcatttgga tcagaaaacc ctgcaactga tgtggagagc
    28441 atggtgatgg cacttgattt cttcaaacag ttggggatca cgcaaatccg gctagtcatc
    28501 aattcattag gtgataaaga aacgcgtcaa gcctatcgtc aagcattgat cgactatctt
    28561 ttaccttttg aatcagagct gagtgaagac tctaaacgcc gccttcatga aaatccatta
    28621 cgcgtattgg acagtaaaga caaacgagat caaaaatttg tggcagaagc tccatctatc
    28681 ttagattatc taagcgagcc tgctcaaaaa cattttgata ccgttgtttc catgctggat
    28741 gcattggctg ttccttatga aatcgacagt aatatggttc gcggactaga ttattacaca
    28801 catacgattt ttgaaatcat gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc
    28861 gcaggcggac gttatgacaa tctagtggaa gaactaggtg gtccttcaac acctggcttc
    28921 ggatttgcaa tgggtatcga gcgattactc ttgactatgg aggcagagga agtagttatc
    28981 cctgcattta atgagttgga tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa
    29041 gcattgaaag tggtccaagc gattcgaaac tttggttttt cagctgaccg cgacttcatg
    29101 aaccgtaaag caaaagcgca atttaagaca gcagataaag cgaatgccaa attagtattg
    29161 gtgatcggtg aagatgagct tgctagtcaa acgattaatg tcaaagcaat ggcaagccga
    29221 aaagaaaaaa gttttccatt agaagagata tatgaacatt ttgatgaagt ctatgatgaa
    29281 atgactttat tcgagtagga ggaaaaaaat aaatggcaca aagaacgatc tattgcggat
    29341 tagtttccga acaacaatta gaacaaacag ttacattaaa aggatgggta caaaaaagac
    29401 gagaccttgg gggagtaatc tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg
    29461 tctttaatcc tgaaatcaac aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat
    29521 acgtgatcga agtgactggg gaagttagaa gacgcggaga aaatgcagta aatcctaaaa
    29581 tggccactgg tgaattcgaa gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa
    29641 ccccgccatt tcaaattgaa gatgatcaag caatcagtga cgaaatacgt ttgaaatatc
    29701 gttatttgga tctgcgtcgt ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa
    29761 caaaagcgat tcgtcattat ttagatgata atgactttct cgatatcgaa acaccttacc
    29821 tagggaaatc tacaccagaa ggcgcaagag actacctagt tccttcacgt gtccatgctg
    29881 gacatttcta tgcgttgccg caatctcctc aattgtttaa acaattgctg atgaatgctg
    29941 gattcgaccg ttattatcaa atcgttcgtt gtttccgcga tgaagattta cgtggagacc
    30001 gtcagcctga atttacgcaa gtagatattg aaacaacgtt cttaacagca gaagaaatcc
    30061 aaacttacac agagggattg attgcgaagg taatgaaaga agttcgcggt atcgaagtca
    30121 cattgccatt tcctcgtatg acctatgatg aagcaatggc tcgctacggc agcgacaaac
    30181 cggatacacg ttttgatatg gaattgatcg atctaagcga tacagtcaaa gaagtagaat
    30241 tcaaagtctt ccaaatggct cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag
    30301 gcgctgcaga tcgctactca cgaaaagaca tggatcagtt aggtcaatat gtaggacaat
    30361 tcggagctaa ggggcttgca tggctgaaag tagaagaaga cggattgaaa ggtccaattg
    30421 ctaaattcat gggagaagca acagaagcaa tcattaaagc aacagatgca aaaccaggtg
    30481 acttgctgat gtttggcgca gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc
    30541 ggacacgttt aggaaaagaa ttaggactaa ttgatgaatc gaaattcaat ttcttatggg
    30601 taactgattg gccacaattt gaattttcag aagaagaagg tcgatatgtc tctgctcacc
    30661 atccattcac gatgccaaaa gaagaagata tcccgttatt ggctgaagac cctgcaaaag
    30721 tctatgcaga agcttatgat atcgtcttga acggttatga actaggcgga ggttctatcc
    30781 gtattcatac tcgtgagctt caagaaaaag tactagaaac tctaggcttt acaaaagaac
    30841 aaatggaaga acaatttggt ttcttgttaa cagctttaga ttacggattc cctccacatg
    30901 gcggtattgc attaggattg gaccgttttg caatgttatt agccggagaa gataatattc
    30961 gtgaagtcat tgcctttcct aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa
    31021 gtatggtttc gcctttacaa ttgttcgaat tgaatattga tgtaacgaat atagaagaat
    31081 agccgataaa aaggtaaaaa ggatttgtcg aatacgcttc ttttgacaac cttgatttaa
    31141 ttgaaaaaca ctttataatg aagccataac atataaagcg gggcttttcc atgaaaaaaa
    31201 cattcgaaaa ttgggcttat catgattaca cgaccaagct ttcggtatct atcgtttatg
    31261 cgttgatcgc atcagtcgca ttgaatttct tttatcagcc aggacatatt tattcaagtg
    31321 gtattactgg tctagctcag attttgacga ctttgtcaaa atcactgata ggattcgaag
    31381 taccggtatc aacaactttg tatttactga atataccttt atttgtattg gcgtggttaa
    31441 aaatcgggaa gaagtttaca attttcactt ttatcacagt cactttgacc tccctttgta
    31501 tccatatttt accggaaaca gtcctatcta ccgatccaat catctgtgcg ttgtttgggg
    31561 gggctgtgat gggactaggt attgggctag cgttgagaaa cggcctatca tcgggcggtc
    31621 tagatatcgt gagtatcacg attcgtaaaa aaacaggaaa gtcgattggt tctatttcga
    31681 tctacttcaa tgcattgatc gtttttgttg ccggaattct atttggctgg caatatatgt
    31741 tttacagtac gttgtctatt ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac
    31801 aaaagaagat gcaggtaatg atcgtaacga aacatccaga aaaagtagta aatgaaatcc
    31861 aaaactgtat gcgtagaggg atcacgatca tcaatgaagc agaaggtgct tataagcatg
    31921 ataagcagac agtattgatg acgatcgtta cacgatacga actgcctcta cttagagaag
    31981 caatgaaagc ttctgatcca caagcttttg taagtattgc agaaaacgtc cagatacttg
    32041 gtcgtttcta tgaggatggc atttagatcg agtttgacaa aaattcatga aatactgctg
    32101 tagacagacg ttcgtttgtc tgcagttttt ttctaccata gtagaatcag caaagaaaaa
    32161 ctgctttttt atttgaaatt ttcttaaagt tgaaaaaaag catgtttatt tttttaaatt
    32221 tatgggatga tagaaaaaaa tagtagagaa aggacacgat tctacttgct attttcactt
    32281 aatacccgta tattacgaat gaaaaaagtt tatgaaaagt tggaatattg aatggaaaaa
    32341 caaaaaaggt taaaaaacag ccgctacatc actgggtttg atgggctccg ttcattagct
    32401 gtggtgggtg ttatcttcta tcaccttctg ccaactagta tgaaaggcgg ctatttgggc
    32461 gttccgattt ttttcgtcgt atctggttat ttgatcacgg atctgctgcg gcaggaatgg
    32521 gaacaaaatg gaaaaatcaa tatttggcaa ttttatgtca gaagaatgaa gagactctat
    32581 ccggcacttg tttttatgct tgtgacagct tcggcttata tcactttgtt tcagcgaggg
    32641 ctgttaaaca atcttcgagg agttgtggtc agcagcttac tgtatgtcaa caactggtgg
    32701 caaatcaata acggtttatc ttatttcgat cgatttgcta atgagtcacc gtttacgcat
    32761 atctggtctt tagcagtaga aggtcaaaac tatttggttt ggccgatctt gtttgtattg
    32821 ttgatggtat ttgttcgtaa aagaaaatgg atcttttata ctgtgttagg tacatccttc
    32881 ctctccgcca tcttgatgat gatcttgtat acaccaggag gcgatccaac ccgtgtttat
    32941 tacggtaccg acactcgtct gttttctata tggttgggaa gtgcgcttgc gtttgtttgg
    33001 ccaagtacgc gtttaaagaa aaacatcccg aaacaagcga aaaatattct gaatatcgct
    33061 ggaggaatct cgcttttggc actgattctt tgcttcttct ttttagatga tcattacaat
    33121 ttcatctatt acggaggaat gctcctagtg agtctgttat gcgtagtgct agttgcagtg
    33181 acagcacacc ccggagcaag tttgaatcga tggctgacaa atccagtatt tacttggatt
    33241 ggcaaacgaa gctatggcat ttatctgtat cagtttccag ttatgatttt ttatgaagca
    33301 aaggtgactg atattgccga tcatgttttg ttgcatactt tagtggaaat cacattgatt
    33361 ctaatcatca gtgaactttc ttatcgatat gtggagcgtc cgctagcacg ttttgattat
    33421 ggtcgtacat tccaagtgat gaaaggatgg ttcagcaaac cgatcatcag taagagcaaa
    33481 ccatgggttg ttccaggcac attacttgta gcaattgcac tcgttggttt cgttacggca
    33541 ccgaaaaacg ctgtaacagc cgatcaaaaa caactgcagg caaaaatcgc tgaaagtaaa
    33601 aaattagcag aagaaaccag aaacaattca gaaaacacca ctagttcggt tgaccaagcg
    33661 atccttgata agtacgagtt gtccaaggca caagggaata aggcacaaaa attagaacta
    33721 acggcatttg gtgattcagt tatgttggat gctgcaacgg atctacaaga actttatcca
    33781 aaagtagtgg tagatggaga tgtcggacga caattatatg ccagtgtacc gtacattgaa
    33841 aaattaaagg aacaagattt gttgaaagac actgttctga tcggactagg aacgaatggc
    33901 tcattctcgg aagctcagtt tgatagtgtg atggatgcaa tcggtgatcg aaaagtatac
    33961 tgggtcaatg tccgagtacc gacgaagcgc tggcaaaatg acgtgaatgc gatgcttgaa
    34021 cagatgacga aaaaatacga caatatgacg ctgattgatt ggtacagcta cagtaatgac
    34081 catagtgact ggttttatga tgatcaagtc catccaaatc ctgaagggat gacacattac
    34141 attcacttag tcagcgaagc cttgctagga ggaagttcaa gtacgaacag cagtgagaca
    34201 aacgtgacag aatgatagct acaaaaaact tcaggcaaaa acatttgttt ttgcctgaag
    34261 tttttttata tttaccgtgt ttttaacatt catatagatt caggaagcaa aacggttgag
    34321 tgtgtctgta aataacgttc tttttgtttt cgggtgagtt ttttgaatgc agcttctgct
    34381 tttgttgctt cgcttcttga agaaaatact tctgcatgga tcatcttaac aggtcttctt
    34441 ttttgaagac gagtatattt agcaccagta ccattgttat gttcctctaa tcgtctagaa
    34501 agatcggttg tatatccacc ataaaatgtc tgatctttac agaataatac gtaaaaataa
    34561 tgttcatttg ccatagagca tctcacgaat ctctgcagtg tattctccat ccatattgta
    34621 agtgatcaat ggtggagcga ctttgaatcc gtctaatttg ccttctttga ttccttcaac
    34681 taacaaaata tttgcctctt ttccaggttt tgggtaaaca aattggatcc ttttaggtgc
    34741 aattcgatag tcttccatga cctgaatgat ttctaagaac cgatcaggac gatggaccat
    34801 agtaaagcgt ccatttgttt ttagtagctt acttgaaata tcgatcactt cttctaacgt
    34861 cgtatgaatc tcatgacggg cgattgccag gtgtttatta gggttcgttt tatttgttgg
    34921 cagccctttg aaataaggtg gattacagac gactaagtca caagaattat gtcgaacgac
    34981 agataagctg tctttcagat cgatggtgtg gacagtcatt tgttcattta aatggttaag
    35041 cgcgatacta cgtcttgcca tgtctgctag acgaggttga agttcgatag cgtcgatagc
    35101 cgcctgggtt cgtttactta aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac
    35161 aatcttccct tttttaggta cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa
    35221 agaaaacact ttggaacttt ggataatctt gatatcttct gcgtataatt ggtcgattcg
    35281 ttcgtcttcg tgaagcatgg attccctcca aaatgaaaaa aatagtcgtc tgtattatac
    35341 tatttttatt aaaaagaacc aaggtggctt gtcaaacctc tattttttat gcatactatg
    35401 accataagaa gggaaaggaa gagactatgt tttatcgatt tatgcgcggg gtagttcgag
    35461 tactattagc cttagttaat ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag
    35521 atgagaatta catactagtt gctccccatc gtacttggtg ggacccatta tatctggcgg
    35581 tagctgcagc acccaaggaa ttcagcttta tggcaaaaga agaactgttc aaaaatccag
    35641 ttctgcgatt tatattaact cgatccaatg cttttccagt taaaagagaa aatccaggac
    35701 caagcgtcat taaaacacct gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt
    35761 ttcccagtgg gacaagacat gctacagaac ttaaaggcgg aatggcattg atttccagaa
    35821 tgtctaaagc gaaaatcgtg ccagctgtat atcaaggtcc gttgacactt ggggatctgt
    35881 tcaagcgtca gagagtgaca gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga
    35941 aaacggataa agaaggtatg gcggagatcg aacgacgaac acaagcagct tttgacaaat
    36001 tggacaaaga agtgaaccct gatttcaaat atgaagtcaa aaaaaaataa aatgataagt
    36061 ttatgcgtgg gcggtctttt gaatctactg tcctgttcag ttcagacttt ttcaatgaaa
    36121 acgagggaga gacgtcaagt ctgccctctt tttgcgttcc aatatgtaaa atagaagaca
    36181 ggtgtcatgt aatggatcaa agtgttataa taattagaag gacatttttt gaggtgattg
    36241 aatgcgtttt ttacatacag ccgattggca tatcggaaaa aaactacaag gttatgactt
    36301 gattgaagac cagcgtcatg tactatcttc tatactagat atcgcgaaag aagaaaaagt
    36361 agaggcaatc gtgattgccg gagacttata tgataggagt gtcccagctg ttgatgccgt
    36421 agaattatac aatgaattga tggtcgattg gaatttaaag gaaaagatcc cgatttttgc
    36481 tatatcagga aatcacgata gcagtacgag actttcagcg ggtacgccat ggttcagtta
    36541 ttcgaactat tttttgcgaa cacagctagc agaagcattt gaaccagtcg agtttaaaaa
    36601 cgtacaattt ttctttcttc cttactttga accaattgca gcgagattat attttgaaga
    36661 tgacaatatc cgaacgatca agcaagcggt agccaaggtg atagataaaa tgcaggagct
    36721 atttgattca gaaaaaaaac aagtattgat tagccatttt tttgtaatgg gaagtgtaaa
    36781 atctgattcg gaaacgaaaa tcgaagtagg cgggttggat tcgatccctg gtgaactact
    36841 tacaccgttt gattatgtag cactaggaca tttgcataat caagctgctt tgagacagga
    36901 taatgctcgc tatagcggtt ccccattgaa attctcttta tctgagatca accaaaaaaa
    36961 aggcgtatgg atcgttgacc tgacaacagg cctggagttg aattttaggg agatcgaacc
    37021 cctgcatgat attgaagaaa taacagcgag tttcaaagaa ttgctggatc ctgcctttta
    37081 tcagtccgtc aatcgggaga attacatcta tatctatctc actgatagaa cgatcatccc
    37141 gaatatgatg aaccagcttc ggcaaatata tcctcgtatt ttggggattg aacgggcaaa
    37201 cggtcgagca gaacaaacaa agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa
    37261 attagcagaa gcatttttta gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa
    37321 atggctcgga gaaacgttgc aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa
    37381 aaaattacaa cttaaaaatt ttggtccttt tatcaatgag accgtagatt tttcacgttt
    37441 gacagaagca ccgctttttt tgatcagcgg gaagacagga gctggcaaaa cgacgatttt
    37501 tgatgggatc accttcgcac tttttggtga aacttccgga agacttcgct ctggaaaaga
    37561 gatgcgttct ttgtttgcta caccagacga agaaacaagc gtgacttttt cttttgaaca
    37621 tcagcagatg agctatgaaa tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg
    37681 aaacggaaca aaaaaacagg cagccaaagt aacgttgacc atttttgatg ggaaaggaaa
    37741 agaaataaaa caaatcacaa aacgtacaga agcagatcag ttgatcagag aactgatgaa
    37801 tctcgatgcg aaacagtttt ctcaaatcgt tttactaccc caaggagaat tccgtaattt
    37861 tttggtttca tcaagcagtg ataaagagac tgttttacgt catttattcg gtactcaatt
    37921 tttccaacag ttcaatgagc gattaaaaga aaaagcaaaa caacagcaac aaaaattgga
    37981 tcatttagag caagagctgc atcttctgca aaaaaggttt gttttagtca aagaagagga
    38041 accagcagca gggtttgaag ccgttttagc ccaatggaca aaccatcagg caatactcga
    38101 aaaacaaatc acggaagaaa aagaagctct aacggaactt caggaaaaac aaaagcaact
    38161 agaaaattcc tactatcgtt ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt
    38221 aataaacaag caaaaggaat tagctgctca aaaagacgaa atagaggaaa agaaacgatg
    38281 gattgggtat tatgaattta cgcaacgatt agtcgaacca atccagcgcc tacaagaaat
    38341 aaaagttgaa aaaatagaac tgtcacaaaa aagtagtgag caaacaaaga aactagcgat
    38401 gactacgcag aattatgaag agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac
    38461 aaaaatcaaa gaagctgctc aaaaactgca aaacaaacag atgatggtgc caattgtcga
    38521 atccttacgt agtaaaaaac aagacgcaga acaattaaca atttcattgg ataaagaaga
    38581 attgaatgcc gagaagctgt atcaaaatct cgctatctat caggaaagaa ctcgaattct
    38641 tcaagaaaat ttactggatc aggaaaaact gcaagaagat cgtcttgctt ttggacagtt
    38701 aaaatatcta gatgaaaagc agcaagaaat agaaaaacaa aaagcaaaag acactgggtc
    38761 gctcgcacag ataagagaag aactcaaaaa agcaactgaa gaaataacaa aaaatagtca
    38821 gctattgaaa gaacagacag aacaagcaga acagctgaaa agccaatggg caaagcagca
    38881 aattgctcgt cttcagcttt ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac
    38941 ggaacacccc atacaacaat taaaacacga acagccaaat aaggaagata tcttaaaagt
    39001 agaacaacaa ctggcagaag ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa
    39061 aagtgaagca catgaaaata atcttcaaca acaattatca gatttagaac aaaaaatcat
    39121 ggcaacgaat atacaattgg ctgaacaaga agaacacttg caacaggctt tgaaaaaggc
    39181 atttcctgag ataacagaac aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt
    39241 agatagacga aaaagcaaga tggccaatgc aagaatagag ctggaagaaa taagacaaca
    39301 agcagaaaag ctcgaacaac aattagctgt ctacaaacag cagcagacag aaaaaaataa
    39361 tcagctccag caactgcaag gagaaatcaa aagtttagaa caacaattgc aagatacgga
    39421 tagtaactgt ttagaagagg agatacgacg acttgaaaaa cagttagaag aagactcgaa
    39481 tcaactagct gttgagcaga aagcaggagt gcaattgttg caagatcttc atacgcttcg
    39541 gacacagctt gatcttcaac atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga
    39601 acaagaaaga atagctaaaa agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat
    39661 ctgtgcattt gcacaacagc aagcacaata taaagaaaac caacgtacag ttacttctta
    39721 tcatgaagag caattagtga cagctgaccg gctgcgtcaa ttagaaaata tccagctaac
    39781 tgagacattc ccagatacag aagagatcag ccaacagtta gaagaaataa gacgtatgat
    39841 cgaaataaag caagaaaaga tatacggttt acaagaacaa aaaattagta atcaaaaaat
    39901 ctacgatgaa tgtctttcga tttatcacac aagccagacg aaattagatg agcttacgca
    39961 attacagcag ctttcgcaaa cattcaacgg agaaaatcct aagaagacta gtttggaacg
    40021 gtatgtgctt caggtttatc ttcaagaagt gttacaagta gcaaatgatc atctccaacg
    40081 tttaacgaaa aatcgttatc aatttgagct agcggatacc attggcagtt atcgagggaa
    40141 aaccggatta gaaatcaata tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac
    40201 cctgtctggc ggtgagagtt ttattgcagc actttcttta gcattagctc ttgccgatgt
    40261 gatccagaca caagctggtg gtgtagcaat cgatgcattg tttattgatg aaggatttgg
    40321 gtcattagat gaagaagctt tggaaatggc aatggaagct ttagaaacga tcgaaagtga
    40381 aggaagaatg atcggcatca ttagtcatgt aagagaattg aaagaacgag tattacaaca
    40441 aataagaatt gatacagaag gtagcggaca aagcaaaata cgctatcaac ttggataaaa
    40501 agggagattg gtaactatga aatggagtat tccagaaaaa gtcattgcac gtggacgtca
    40561 atacttgcaa gaaggccgag tgttatcggt tgtccctgac caagaagaaa acgtgtggca
    40621 tgcagaagtc ttgggcagtg aattgtatat ggttgatttg gatggtacag caaaagagat
    40681 agattactgc caatgtccct attgggagga acataaatat tgtaagcata cagttgctgt
    40741 ggaactttat ttacggtcga aaaatctatc tcgtatcatg aaaaaagacc agccaatgct
    40801 agaaccggta aaagcaacaa gtgagggaga aatcattgca aaaggttttt ctcgtctaaa
    40861 aacacaaaac aatcctcaag cggtgcaacc tttgattatt gaataccaag tagaaacgat
    40921 tgagactaat cagtatcacc cagagctttc tattttggca ctttacttgc gtatcggttc
    40981 attggcatca ccgaaaaaaa catatatcgt aaaaaacatc tatgattttt tacaagctta
    41041 cacagaaaaa gaaacttaca ctgttaataa acagtaccag tttcgtctag ataaacgggc
    41101 gtttcaaaaa gaagatcaaa aaatactcac gcatcttgca ggaagtgctc agacgcatca
    41161 attattaggt acgaacggct tacaagtcaa aggaaaacta gataaaagat atctgcttct
    41221 cccagtagaa caagcccgct tcctgttaga gcagatgaat cagactacac gtttgcatct
    41281 gcagataaat gaacaaaacg atcaaggaat acaattttcc gatcaaaaca aacctttatc
    41341 attcaaagtg gaaaaatttg gagaagatta tttgctgcaa gcattgaatg attttgattt
    41401 tttctttatg cattatcagt ggggagttat ccaagggaca ttttattctc tgacgaaata
    41461 tcaacaaacg gtttatcaaa cttggaaaca attgattcgc cgattggaga aaccagaagt
    41521 agtgttcaag aaaaaagaat taccgtcttt gtttaaagag attatccctc tgctgtcaga
    41581 gatcggcgac gtagctattg cccctgcagt agaagaggaa gtagcagata tccccgtcga
    41641 atttattttc tttttccgaa aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta
    41701 tggcgaagtc atctattcga ctgacccgaa gcatgaggtc tcgacagaac aggcttttca
    41761 ggtgttacgt gacacagtag aagagaaacg agtacttgat ctattcagaa tgtatcgcta
    41821 ccagaaaaat gaaactggtt atgaacgtcc attgccaagc ggggaagaac tatatgcctt
    41881 tttccgcaca gaactatctg tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt
    41941 gcgagagttg tatttagatg cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg
    42001 ttcatggctc gatattcgat tcgatgtgac agggatagag gagcaagaaa tcgatcatgt
    42061 cctgcaaagc ttgctgagaa atgatgcttt ttatacatta gaaaatggtc aggttctttc
    42121 ttttgattca gaagaatttc aacaaacaag ccaagtgttg caacaattac gggaatcgat
    42181 acgtacagaa gaaggaacga tccatgtacc taaaaatcaa ggattgatca ttcaaaatca
    42241 attggaaaaa agcaatgcta cttttagtga atcgttccaa acgatggtac aagatctcat
    42301 tcatccagaa agataccagg cacagcttcc caaaggattg aatgcaacga tgagagatta
    42361 tcaaaagcaa ggttttcgat ggttgaagat gttaggtcat tatcagtttg gcggtatttt
    42421 agccgatgaa atgggacttg ggaaaacact acagacaatt gcctttttac tttcggaaaa
    42481 agaagaacgg aaaagcttct ctgctttgat cgtagcgcct gcgagcttaa tttacaactg
    42541 gcaggcagag gtgagaaaat tcgccccttc tttaagtatc caagtaatca atgggaataa
    42601 aaaagaacgt gaagaattac tggctaaaga cacagatatc cgagtgactt cttatgccag
    42661 cttaagacag gatctagcta attatcagtc gcaaaaaatt gattatttga ttcttgatga
    42721 ggcacaaatg gtcaaaaata gcagtacgaa aactgcacaa gcattgagag aattagctgt
    42781 acctcagcgg tttgcactga gtgggacacc aatcgagaat aatttagaag aattgtggtc
    42841 attgtttgca acgattatgc caggattttt cccgactaaa acaaaattca gggaacttgc
    42901 cgcagaagaa attgcgcaaa tgattcgacc gtttatttta cggcgagata aacaaactgt
    42961 gttgaaagac ttgccggaaa aaacagagat gaatctatat tctgcactta cagaagaaca
    43021 aaaaactgtt tatttagctt acttgcgtca aatgcgtgaa gaaatcacat caatggattc
    43081 tgaagcattc aagaaaaacc ggatcgggat acttgctggt ctgacaagac tgcgacaaat
    43141 ttgctgcgat cctcgtctgt ttattgaaga ttaccaaggc ggttccggca agctcgaaca
    43201 agtaaaagat ctactggtag ctgccaaaga gaacaaacga agagttttgc tgttttctca
    43261 atttacaggg atgctgacta tcttgcaaga agagttagcc gagctaggga tctcgacatt
    43321 ttatttaaga ggcagtacga aaccacaaga tcggttaagt atggtagacg catttaatgc
    43381 tggcgaaaaa gatgtttttc tgatttcctt aaaggctggg ggaactggat tgaatctcac
    43441 gggagcagat acggtcatct tgtatgacct ttggtggaat cctgccattg aagaacaagc
    43501 tgctggacgt gcacatcgga tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc
    43561 tgaaggaacg attgaggaac gcatggattc actccagcaa gaaaaacgcg aactgttcca
    43621 aaaagtgatc caaggtaatg aagaacagtt gacgaaactg actgaagaag acatccgcat
    43681 gattttaagt gtaggagaaa tcgaggagta agagactcga aaaagcgttc agtaatgggt
    43741 aagaaagcct ttctaaagaa gcgattgttt atttgattta aaaaatagca aggaggctgg
    43801 gattccattt tatcccagct tccttgctat cattttttta tttctatttt taaaggttat
    43861 acaaagattg tcttcattgt ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa
    43921 attggagtct gttcaatttt gcgttctcga tggttgaacc aaagcgcttg ccagttagca
    43981 ctttttgcac ctagaacatc attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga
    44041 agcatatgaa attctttctc agctagctga aaaatctctt tttctggttt ttggaaacct
    44101 gttgcttgag aaatgagcat attgcgggaa ggaatccaat tgttcaattg aagttgcttc
    44161 agtttttttg tctgatggtc agtcggccca tttgtgataa ttcctaatgg gacattcttt
    44221 tccttcaaga aatcgagggt ttttttgacc tcctcatgca agcagatatt atcaagttct
    44281 tcctcgtaaa ttttttgaaa taagaggcca tcttcttccg taatatgagg gtagtctaaa
    44341 tcttttagtg attggctgat ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc
    44401 attacttttg ggaagttttc atcactatga tggcggaacc ggatatatag tggatgcata
    44461 tctgcatctg aaacaagcgg aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga
    44521 tcatacatcg tatcatcaac atcaaacaca actgatttaa tcaaaatttt acacgccttt
    44581 cggttttcat tgattttttc acaaatcctt gactattgta acagaaaaaa ttgtctaaaa
    44641 aagctgataa ataaaggttt ttcttactat atttctattt ccttgacaga ataaggtttt
    44701 tatatttcag tttttttcac taaaaatcgt gaaacacgtc aataagtttg atgaacaaat
    44761 taatcattat ggtagaagtc gattgattta ttttaatgca atctctgtgg aattttcgaa
    44821 agatttattt ttttaataca gtctcaatat aactattcaa acataaaata acgttatttg
    44881 atttatttgt ttatagtaaa ttttatttag taatcattgc tttaaaatac tacgcttata
    44941 taacagagaa aataagcgtt ataataaaga acagtattat ttaaaatgac agaaaacatt
    45001 actattaaag aaataaaaaa cataaaaaat cttaagaaat cggtttcatt tagcttttga
    45061 gataaaataa tagtacagtt ttatacgctg tttcatatgt ttagaaaatt tgaattagat
    45121 ttgaaaactc taatttttaa atgataaaaa aagcgagaat gctggtttta ctgtatatta
    45181 accttagata actttcattt ttttaaaaaa attatgaaaa aaaggtagct atttaaaagt
    45241 tgattatgta taatagaaac agatgggaga ttattatgat cttctgttac aaaaaagtga
    45301 ttcttatcac gaaaaggagt ggtgcacgtt gttaacatta tatacttccc caagttgtac
    45361 ttcctgcaga aaggctcgtg cgtggttgca agaacatcag attccatttg ttgaacgtaa
    45421 tattttttca gaaccattga acagttctga attaaaagcg attttacaaa tgacagaaga
    45481 cggaacagaa gaaatcatct cgacacgttc caaagtattc caaaaactga acatggattt
    45541 agatgatctg ccgttacaag aattattgga acttgtgcaa aacaatcctg gattgcttcg
    45601 ccgtccaatc atgattgatg ataaacgtct gcaagttggt tttaatgaag atgaaattcg
    45661 tcggttccta ccgcgcgatg tacgacaatt agaattacgt caagcacaat tgatggctgg
    45721 attatagaag gataatacga aacttggaaa agactattac aaagatagtt tttccaagtt
    45781 tttttgtttt tacaaaaagt attcttgatt agatgtataa aaaggtagta gaagatgatg
    45841 attgcacgaa aaatagagag ggaaaattag ctgaaaagtc ggctatatag tattcgtctg
    45901 ccaaaaatgt tatagttaaa taagatagct tgaaaggttg aaaaatccgc tggttttatt
    45961 aaattcgctt aacagcgaga ttttttttct ttacatttaa gctgatttcg ctacaatgaa
    46021 tctatattaa acattgtggc aaagtgaggt gtagcaaaat ggaaatggaa catattaatg
    46081 aaaatacgat ccgtgtacta attggtcatg aagatttaga ggaacggggc gtttcatttt
    46141 tggatttact tggcaatcac aaagaaatcg aaaatttctt ttatagtatt ttggaagaag
    46201 ttgatatcga agaggagttc cgaggaagcg aagctgtcac tttccaagtt ttgccaaaag
    46261 gagacggatt agaactgttt atcagtaaaa atcttccgga tgaagaaatt tctcaatcag
    46321 aagattctac tgttgattca gaagatgtga gtgactttct tcgtcagcag attattggca
    46381 gcgatccgga ggaagaatta tctggaagcg ccctagaaga acaggaaaga caagcaactt
    46441 ttgcttttga agattttgaa caagtgatcc aacttgcaac agatgtcgaa ctagagtctg
    46501 cttggactga tttgtattta ttcgaagagc gctattacct agtcgttcac ttctggatgg
    46561 aaaatctcaa ccaagcggat gtagagaatc agattgctcg aattttggag tttgctgaaa
    46621 agagcgatcg tacaccagaa acattgaatg aatatggaca atgtctgatg gcgcgtaatg
    46681 ctatcgagcg aacgaaattt tattttgtat aaatcagcat tatagtaaga gactgtgaca
    46741 aaaattgtgt cgcagtcttt ttttcgtata aatgtgttca aaagcttgca tctgcggtaa
    46801 caagcccaac caaacgaaaa atattgggaa ctatttttgt ttagctgttc ttattacttg
    46861 atgcagaata gctttttcac gccctctttt ttcgtgtaaa taaattctta gacgtaaata
    46921 agttcagagg gagaaaaatg ctgcttgcaa tgaatgaata taataagtgc tgttttgcta
    46981 aacatgcaga aaaaggaaag aaatattatt gtcgtggctg tcgaaaaaga gtgattttga
    47041 aaaagggaaa gaaaaaatgt gctcattttg cccatcaaaa atcagataac tgttcggtct
    47101 ttagtgaaag agaatcaaaa gagcacttac agttaaagga atgcttcatg gattggctgg
    47161 gacagtcagt agaaccagta tttttggaag cctatttgcc gaggttgcgg caaaggccgg
    47221 atattttgtt ggcgaatcta gcaatagaaa tccaatgttc tagactgtct caccagagat
    47281 ttattgaaag aacccaaaat tatttgaata acagttacca agtatggtgg atacttggta
    47341 attcttttct tggacaatcg caattctcat tgatagaaaa aagttgctgc tactataata
    47401 gaaaaagagg agtgcattgc tggaaagctg atctcaagaa acaaaagctt tatttgtatc
    47461 atcatattac agagactgta tcagggcata tttccttttg cagtagctgc tggacttttt
    47521 ctagtaggga ccttaaagaa atattcacta gtaacgaaat taaaatgaac caaatgaaaa
    47581 aaatagaaaa agtgagtgaa gatgggaaga aatggctgac acgccaactt attcacaagc
    47641 aaaagaatac agtatccata caagaacaat gctacttgcg tcataaacat cttttgcatc
    47701 tatcatcgtg gatctatcaa aaaagtcggt ttttctttta tttacaagaa caggtttttc
    47761 tttatcgaat gttgtacgaa gaagccttgg aaaaccagaa agttcctgat ttcaactcat
    47821 ggctttgcca aataaaagaa cataaaaggg aatggctttt tcctatgatt gatgaagaga
    47881 tggtctatca gcagtttttt aacgaatgca tacacttgtc ttctttgaaa aaatagtaag
    47941 tataaaaata ggctaggaat cggttttcta tgtaaaacaa gatttagtta tgctaaaatt
    48001 gatggaagca gacacatgaa tgagtagaaa agttagatgc tgtgacatgt ggatgttaga
    48061 ctatgatcta ttttgataga tagagctaag ggaagaaaag gagtggattt catggaagta
    48121 aagcagttgc caaaacgaga agaattgcct gaaaatttaa cttgggactt gaccaagatc
    48181 ttttcaagcg accaagagtt tgatgagaaa tatttggaat tatcagaaga gttaaaacaa
    48241 tctgaaaaac acaaaggaac acttgatcaa ggcgcttctc aatttttaaa tgcgattgaa
    48301 ttcgtattga gggtttatcg ccaaactgaa gtcatttatg tatatgcgca ccttaaaaac
    48361 gatcaagaca ctggaaatac agattaccaa gcgctttatg caagagcaag cagtctgttt
    48421 tcgaaagtta gtgaagccgt ttcctggttt gaaccagaaa tattgcaatt gtcagatgac
    48481 cagatttggc aatatttcaa agaagaacca aaattggaag tctatcgcca ttatatccag
    48541 caaatagtag ataatcgagc ccatgtctta tctgctgagc aggaatctct tcttgctgga
    48601 gcaggtgaaa tctttgatgc ttcaagtgat acatttgctg ttttgaataa tgcagatcta
    48661 gtttttccaa cgattgaagg agaaaatggt gaaatagtcc aattatctca tggcgtgtat
    48721 ggtcagttgc tagaaagcac ggatcgaagg gtgcgcgaag cagcatttaa ggggttgtac
    48781 agtgtttacg aacaatttag aaatacattt gcttctactt taggcacaca tataaaagga
    48841 cataatttta aagcgaaagt ccgtaattac agctctgcca gagaagcgtc tttgagcaat
    48901 aatcatattc ctgaaagtgt atacgatact ttggtagacg tggtaaacaa gcatttgcct
    48961 ttgttacatc gatacatgga attacggaaa cgtttattag aagtggaaaa actgcacatg
    49021 tatgatcttt atacaccggt cttaggggaa gctccaatta cctttacgta cgaagaagca
    49081 aaagaaaaag ctttagaagc actgaaacca atgggtgaag aatacatggc catcgtagaa
    49141 aaagcattct ctgaacgttg gatcgatgtt gtcgaaaata aagggaaacg aagcggtgct
    49201 tattcttcgg gaagctatga cacaaatcca tatattttat tgaattggca tgatacgctg
    49261 gatcagctat ttacgcttgt ccacgaaatg ggacatagtg ttcatagtta tttcactcgt
    49321 tcgaaccagc cttatgtgta cggcgactac tccatctttt tagcagaaat tgcttcgaca
    49381 acgaatgaaa atatcctaac ggagtattta ttggaaacag aaaaagaccc tcgtgtacgg
    49441 gcttatgtac tcaaccatta cttggatggg tttaaaggaa cagttttccg ccagacacaa
    49501 ttcgctgaat tcgagcattt catgcatacg gaagatgaaa aaggtgtgcc attgactagt
    49561 gaatacctaa gtgatagtta tggtaaattg aatgcaaaat attatggtcc agcagtcgaa
    49621 gaagacccag aaattaaatt cgaatggtca cggataccgc atttttatta taattactat
    49681 gttttccaat actcgactgg cttttctgct gcttcagcac tggcgaagaa aatactaaac
    49741 caggaaccag aggcactaga gaactacttg gcttacctaa aagcaggtaa cagcgattat
    49801 cctgtggaag taatgaaaaa agcaggagtt gatatgacac aggctgcata tattgaagat
    49861 gcaatgtcga tgtttgaaca acgtctaaat gaattagaag aattgatcga tcgtttataa
    49921 ggctttctat ttttgatttt ctcgaataga aacgaaaaaa atacgtcggc tctcttgtaa
    49981 gggagtcgac gtattttttt a
//
LOCUS       DAZAFF010000008        49986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000008 DAZAFF010000000
VERSION     DAZAFF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.008"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..612)
                     /locus_tag="ACG9BB_000492"
     CDS             complement(34..612)
                     /locus_tag="ACG9BB_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN2836465.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(689..1219)
                     /locus_tag="ACG9BB_000493"
     CDS             complement(689..1219)
                     /locus_tag="ACG9BB_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFN2836466.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1223..2083)
                     /gene="rlmB"
                     /locus_tag="ACG9BB_000494"
     CDS             complement(1223..2083)
                     /gene="rlmB"
                     /locus_tag="ACG9BB_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFN2836467.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2076..2471)
                     /locus_tag="ACG9BB_000495"
     CDS             complement(2076..2471)
                     /locus_tag="ACG9BB_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFN2836468.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2468..3880)
                     /gene="cysS"
                     /locus_tag="ACG9BB_000496"
     CDS             complement(2468..3880)
                     /gene="cysS"
                     /locus_tag="ACG9BB_000496"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFN2836469.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3877..4413)
                     /gene="epsC"
                     /locus_tag="ACG9BB_000497"
     CDS             complement(3877..4413)
                     /gene="epsC"
                     /locus_tag="ACG9BB_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFN2836470.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4499..4738)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4735..6195)
                     /gene="gltX"
                     /locus_tag="ACG9BB_000498"
     CDS             complement(4735..6195)
                     /gene="gltX"
                     /locus_tag="ACG9BB_000498"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFN2836471.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6365..7522)
                     /locus_tag="ACG9BB_000499"
     CDS             complement(6365..7522)
                     /locus_tag="ACG9BB_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFN2836472.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7556..8926)
                     /gene="radA"
                     /locus_tag="ACG9BB_000500"
     CDS             complement(7556..8926)
                     /gene="radA"
                     /locus_tag="ACG9BB_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFN2836473.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8989..9516)
                     /locus_tag="ACG9BB_000501"
     CDS             complement(8989..9516)
                     /locus_tag="ACG9BB_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFN2836474.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9746..10552
                     /gene="proB"
                     /locus_tag="ACG9BB_000502"
     CDS             9746..10552
                     /gene="proB"
                     /locus_tag="ACG9BB_000502"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFN2836475.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10549..11796
                     /locus_tag="ACG9BB_000503"
     CDS             10549..11796
                     /locus_tag="ACG9BB_000503"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFN2836476.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11872..12216
                     /locus_tag="ACG9BB_000504"
     CDS             11872..12216
                     /locus_tag="ACG9BB_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836477.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12333..12659)
                     /locus_tag="ACG9BB_000505"
     CDS             complement(12333..12659)
                     /locus_tag="ACG9BB_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836478.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13274..14476)
                     /locus_tag="ACG9BB_000506"
     CDS             complement(13274..14476)
                     /locus_tag="ACG9BB_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2836479.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14593..15108
                     /locus_tag="ACG9BB_000507"
     CDS             14593..15108
                     /locus_tag="ACG9BB_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN2836480.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15307..15753)
                     /locus_tag="ACG9BB_000508"
     CDS             complement(15307..15753)
                     /locus_tag="ACG9BB_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFN2836481.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15800..17278)
                     /gene="msr(C)"
                     /locus_tag="ACG9BB_000509"
     CDS             complement(15800..17278)
                     /gene="msr(C)"
                     /locus_tag="ACG9BB_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFN2836482.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17632..18261)
                     /locus_tag="ACG9BB_000510"
     CDS             complement(17632..18261)
                     /locus_tag="ACG9BB_000510"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN2836483.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18245..18709)
                     /locus_tag="ACG9BB_000511"
     CDS             complement(18245..18709)
                     /locus_tag="ACG9BB_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN2836484.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19196..20464)
                     /locus_tag="ACG9BB_000512"
     CDS             complement(19196..20464)
                     /locus_tag="ACG9BB_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN2836485.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20695..21432)
                     /locus_tag="ACG9BB_000513"
     CDS             complement(20695..21432)
                     /locus_tag="ACG9BB_000513"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN2836486.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21499..22068)
                     /locus_tag="ACG9BB_000514"
     CDS             complement(21499..22068)
                     /locus_tag="ACG9BB_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFN2836487.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22196..23068
                     /locus_tag="ACG9BB_000515"
     CDS             22196..23068
                     /locus_tag="ACG9BB_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN2836488.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23410..24282
                     /locus_tag="ACG9BB_000516"
     CDS             23410..24282
                     /locus_tag="ACG9BB_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN2836489.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24279..26237
                     /gene="nagE"
                     /locus_tag="ACG9BB_000517"
     CDS             24279..26237
                     /gene="nagE"
                     /locus_tag="ACG9BB_000517"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFN2836490.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26569..27420)
                     /locus_tag="ACG9BB_000518"
     CDS             complement(26569..27420)
                     /locus_tag="ACG9BB_000518"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN2836491.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27543..28010
                     /locus_tag="ACG9BB_000519"
     CDS             27543..28010
                     /locus_tag="ACG9BB_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFN2836492.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28273..28755)
                     /locus_tag="ACG9BB_000520"
     CDS             complement(28273..28755)
                     /locus_tag="ACG9BB_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN2836493.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28855..29718)
                     /locus_tag="ACG9BB_000521"
     CDS             complement(28855..29718)
                     /locus_tag="ACG9BB_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN2836494.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            30316..31191
                     /locus_tag="ACG9BB_000522"
     CDS             30316..31191
                     /locus_tag="ACG9BB_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFN2836495.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(31336..31488)
                     /locus_tag="ACG9BB_000523"
                     /pseudo
     CDS             complement(31336..31488)
                     /locus_tag="ACG9BB_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(31756..33087)
                     /locus_tag="ACG9BB_000524"
     CDS             complement(31756..33087)
                     /locus_tag="ACG9BB_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN2836496.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(33236..33949)
                     /locus_tag="ACG9BB_000525"
     CDS             complement(33236..33949)
                     /locus_tag="ACG9BB_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2836497.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            34124..35548
                     /locus_tag="ACG9BB_000526"
     CDS             34124..35548
                     /locus_tag="ACG9BB_000526"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN2836498.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            35847..36554
                     /locus_tag="ACG9BB_000527"
     CDS             35847..36554
                     /locus_tag="ACG9BB_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFN2836499.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            36541..36864
                     /locus_tag="ACG9BB_000528"
     CDS             36541..36864
                     /locus_tag="ACG9BB_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFN2836500.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(36953..37990)
                     /locus_tag="ACG9BB_000529"
     CDS             complement(36953..37990)
                     /locus_tag="ACG9BB_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN2836501.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(38311..39294)
                     /locus_tag="ACG9BB_000530"
     CDS             complement(38311..39294)
                     /locus_tag="ACG9BB_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFN2836502.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(39709..40479)
                     /locus_tag="ACG9BB_000531"
     CDS             complement(39709..40479)
                     /locus_tag="ACG9BB_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2836503.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(40472..41407)
                     /locus_tag="ACG9BB_000532"
     CDS             complement(40472..41407)
                     /locus_tag="ACG9BB_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2836504.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(41723..43372)
                     /locus_tag="ACG9BB_000533"
     CDS             complement(41723..43372)
                     /locus_tag="ACG9BB_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFN2836505.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(43489..46092)
                     /locus_tag="ACG9BB_000534"
     CDS             complement(43489..46092)
                     /locus_tag="ACG9BB_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFN2836506.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(46104..47153)
                     /locus_tag="ACG9BB_000535"
     CDS             complement(46104..47153)
                     /locus_tag="ACG9BB_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN2836507.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(47179..47493)
                     /locus_tag="ACG9BB_000536"
     CDS             complement(47179..47493)
                     /locus_tag="ACG9BB_000536"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFN2836508.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(47509..47955)
                     /locus_tag="ACG9BB_000537"
     CDS             complement(47509..47955)
                     /locus_tag="ACG9BB_000537"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFN2836509.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(47956..49878)
                     /locus_tag="ACG9BB_000538"
     CDS             complement(47956..49878)
                     /locus_tag="ACG9BB_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN2836510.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT        14665 a        10339 c         8685 g        16297 t
ORIGIN      
        1 aataaataag aagctcatta agatttttct aaattattcc tcattataaa atttcaagtg
       61 tttttttatt ttgagtttta ctctcccgta agcgctcatt acttgcttgt gatccattgc
      121 taaacattca gccatgtcta caaaatcaac gccttgcata taataatgaa aaattttagc
      181 ttctattttt gaaaactgga tttcgcaatt ttcgattgct tcttttaata aaagatgctg
      241 aattgctaaa ggttcttgca atgtgatctc tgctaaaaag tctggtcctt ctgtctctat
      301 tttttggtcg attgagactt catctttttg atcacgacgc ttcagcgcgt tctgcttcct
      361 caacagagaa tagatttgat tgcttataac gcttttataa taacttccaa agctaacacc
      421 tttgcttttt tcgtaagttc tcaatgcctg atataaagcg atccttgctt cttgttccca
      481 atcctcagaa acataatttc ttaaataata ggtgtttttc aaacgtagta aaagtggaaa
      541 atatttttga tacagggcag caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa
      601 ctcctcagac ataaaataat tcctccataa attcgtttat ggaggaatct tatcatcaat
      661 gattcatcga actgattaga acgatatgtt atttattttt tctcattaaa tcgtccaatt
      721 tttgggacaa tttttttaac tgctcttcat tccatggaga attacgtcgg aaatcaataa
      781 aatgcatatc tttattttct tgggctattg ttttttccgt tttttttatc gtcttatata
      841 attcataagc agaagttcgt aatgctcctt gggaaaagat catccactgt tctgctaaat
      901 cgcttgtcgc cactgtcact tgtgtcaaac ggtcattcag ctgaccagcg attctctcga
      961 tataactgtc agccgtttcg tcttctttcg taaaaacaac atccaactgg tatttcttat
     1021 aattttgttg gatgcccgga actagttggg catcgaagac aacgatgact tccattcctt
     1081 catatttcgc ataatttgat aaacgatgta aaagagcttc tcttgcatcc gccattttgt
     1141 cctgcttttt aagctggaca agttccggcc acgaaccaat catgttgtag ccgtctacaa
     1201 tcaacagctg ttttttcatg gtctactccc cctgacgttg ggaaaaagcc tgatacataa
     1261 gcaaaccagc agcaacactg gcatttaagc tctggacgtg cccggtcatc gggatcgtca
     1321 gcaattcatc catttctttt ttcaatcctg gactcattcc tttgccttca ttaccaatga
     1381 ctaaagcaat tgatcctttg gcattccatt tttggaagtt tgtgccggtc atatctgtgc
     1441 caaataccca aaatcctgtt tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc
     1501 gtgcaacagg gacatgttcg actgccccag tggaagtctt tgttacgata ggtgtgattc
     1561 caactgctcg gtgttttggt atgatcacac catcgacgcc acttgcgtct gctgtccgta
     1621 aaatcgaccc aaaattgtgt ggatcttcga tgctatccaa aatcaaaaag aaggggtctt
     1681 cttttttctc ttttgttcgt tcaatcagtt ggtcaaggga taaatattca taaggtgtaa
     1741 tcgcaagaac gactccttga tgcacgccgt gatcgctcaa agtttctaat ttttgtttag
     1801 gaacccattt gactggcaca gcttgttcac gtgcgatttc tttcagttct tctacttttt
     1861 ctcctcgtga atcttcttga agaaagagtt tattccctcg cccttgttgt agcgcttcta
     1921 ctgcggcatg gtgcccaaag acgaaattat cttctaatgc ttcttcattt tgatcagaaa
     1981 ctgactgatt tcgtccagtt tgctttttat ttgaatatct attttgtttt ttgtcttcaa
     2041 atccacgttt cgattgatgt ttgtttgttc tttctttacc catttttttc accaactttc
     2101 tgaatacacc agcggatcaa ttcttccatc cgctcggttt gttttgtcaa atgcagatag
     2161 cccatgagcg cttcgaaccc tgttgcgacc cgatacgtcg taacatcagc attttttgcg
     2221 ctcgtatggc ttttagcatt tcttcctctg cgataataaa gttcttcttc ttctgtcaaa
     2281 agatgttctt ccagcatttg ctgaatcaaa aaggcttgtg cattcgcaga aacataattc
     2341 gttgccgcac gatgcagctt attgggtttt gtttctcctt gatcaatcag gtggtcacga
     2401 acatagattt cgtaaatcgc atctccaaca taagccaagg ctaaaccgtt caattgtgtg
     2461 taatctctca tgcttctctt ctccatcggg ttccttgcgg tgtatcttct aataagatcc
     2521 ctttttcttt caacaaatca cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg
     2581 cttgatttcg ttcttcgatc aattgttcaa tctcttcatc taataactct tctgctgtaa
     2641 ataaaatacc gaagatcgaa agccattgcg agaataaagc ttctccttgt gccaagacag
     2701 cggcagaaac tttcggctgg tctagataag tgttcagcca cttagcaagc tcatagacca
     2761 ctgtgatccc atttgctgca ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta
     2821 aattttctaa caaagcgatt ttatccgcat catctgccaa attctctgag gcagtttctt
     2881 ggcggaattt caaattgtca aacgtattct tcagtcgttg taaattggtg gctgcttctt
     2941 tcatcgtcgt ttcactgtag cggatcggac gacggtattg tgtggtcgcc atgaagaaac
     3001 gcaagacttg tggatcaacg tttttgatca tttcatgtac agtgatgaaa ttccctagag
     3061 atttactcat tttttcatcg tcttcgccaa tcgttacata gccattgtgc atccagtaat
     3121 tagcgaattt tttccctgtt ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa
     3181 attctaaatc ttgcccgcca ccatggatat cgatcgtttc tcctaaatgt tttgttgcca
     3241 taacggaaca ttcaatatgc caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa
     3301 tctcgccagg ttttgcttgt ttccataaag cgaaatctaa tgggtcttct ttttgctgct
     3361 gttcttcccc tgtacgctga cttgctccga cttccagttc atcgatggat tggtcactta
     3421 atttcccata attttcaaat ttacgggtac ggtaatacac atcacctgaa gattcataag
     3481 catagccttt ttcaaccaac acttgaataa atgctaaaat atctggcata tgatccatga
     3541 ctcttggatg cagtgtagca ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc
     3601 ggatgaaacg atcagcaact tctggtgctg taatacctaa ttcgtttgct gtacggatga
     3661 ttttatcgtc tacatccgtg aaattcgata cgaaatcgac tttatagcct cgatattcaa
     3721 aatagcgtcg aattgtatcg aaagcaatgg tgctacgagc atttccgata tggatataat
     3781 tatacacagt cggtccgcag acatacatac cgacatgtcc cttttgtata gggatgaatt
     3841 cttctttttt tctggtcaat gtattgtaga tttttatcat tctgatctcc tcactttttc
     3901 tcctttgatt cgaacgactt ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc
     3961 tttcaacaca acggcacctg caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa
     4021 tatctgcgca ttcgcagaga gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt
     4081 ttcttttcct gtgccgccta atgttacgcc atggaataaa atcacatcgt cctcgatttc
     4141 tgcggtttcg ccaatgacga ttcccattcc atggtcgatg aacacgccag ttccaatcgt
     4201 tgctcctgga tggatctcaa caccagttag gaaacgccag aattgagcat tgatcttcgc
     4261 caataaaaag agccggtgct gatataaaaa atgagagaaa cgatgccaaa acaaggcatg
     4321 aaaaccaggg tatgtcaaaa cgatctcagc tgtcgaacgt gcagccggat cgtttctctt
     4381 tgctgcatca atcgctcggg taaaccaact catgactttc cactcctttc gtaagaattt
     4441 tatgtaggaa atcatcttct tataaagaaa agaagcgcct tttaaataag tttccttatc
     4501 aaaagacgct ttccgcgtgg ttccactttt cttcataatc aatgattatc tctgacagct
     4561 aacgcctgtt atacgttctt ggctactttg tttcctataa aacagttttt cctgttttct
     4621 gtgtttcgcc aagaccactc ccagaggcat ttcggcaacc attcattgat accttgcacc
     4681 acccggtatc tctcttgtaa tgatcagctg cttacttttt tctgttcatc gtgtttagtt
     4741 cagtacttga tttaaatgtg ctaatgattt ttcacgacca agcaattcga tcgtttcagg
     4801 caattctgga ccatgcattt gtcctgatac agctacacgg attggcatga aaagattttt
     4861 tccttttacg ccagtttctt tttgaacagc tttgatggca gctttgacag ttggagcatc
     4921 tactacttcc atttgttcta gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc
     4981 accagcaagt acttcttttg ctgcttcatt caatacagga tgttcattga aaaatagcgt
     5041 agacagatca acgatttctg cagcatagct catttgaggt tgatacaggc taacgatctt
     5101 cttcacccat tcgattgtct tttcatctgg gttttctgct acacgtccat cagcgattag
     5161 ataaggcaga cacatgtcag taaatgcatc tagatccatt gctttgatgt aatggttgtt
     5221 tacccattgc agttttttcg catcgaaagc tgcgggtgat ttacttaggc gttttggatc
     5281 gaacattttg atcaattctt ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc
     5341 caataatgca ataaagttga acattgcatc tggtagatag cctaattcgc gatattgttc
     5401 gataaattgc aagattgatt cgtcacgttt gcttagtttt ttacctgttt cactgttgat
     5461 gatcaatgtc atatgtccga aggttggtgg tgtccagcca aatgcttcat agatcatcag
     5521 ttgttttggt gtattggcaa tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa
     5581 atgatcatcg accgcaacag caaaattata agttggcata ccatcgcgtt tttggatgac
     5641 aaagtcgcca ccgatattat cggattcgaa cgaaatagcg cctttcacca tatctgtaaa
     5701 tgcgtattct gtattgcgag gcacacggaa acgtacaaca gattctagac cttgtgcttc
     5761 tttttctgct tgttcttcag gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc
     5821 tcctcttgcg cgttgtgctt cccgttctgc ttctaattct tcttctgtgc aatagcattt
     5881 gtatgctcga ttgctagcaa gcagctgatc aatcagcggt tgatagattt ctttacgttc
     5941 agattgacgg taagggccgt attctccagg attttcagga gattcatccc aattcattcc
     6001 caaccaagca aggttttcca actggctttt ttcgccatct tcgatattac gtttttggtc
     6061 agtatcctca atacgaataa taaagtctcc atcattgtgg cgggcaaaca aataattgaa
     6121 taatgcagtt cttgcatttc caatatgcag gtgcccagtt ggacttggtg catagcgtac
     6181 gcgtactttc gtcattttct tcctcttctt tcaaactaga ctttgactaa cagcaaaaat
     6241 tccgtcagtt acggcttctc aaatatcatt tatttaaagc cactcagcac aaattttacc
     6301 ttttctaagg taaatagtaa agccaactgg ttgaatttcc ttaccagttg gctgaatttc
     6361 ttttttattt tgattgttta gtctttttct ctgtctcttt tttattgtcg attcctcgtc
     6421 ctgcatgtgc aggtttagca aagatcatgc gtccagcagc tgtttgaagg gcacttgtta
     6481 caacgacttc gatacgttca ttcatgtaat gctgtccatc ttctacgaca accatcgtgc
     6541 cgtcatcaag gtaggctact ccttgttggc gttctgtccc ggcttttact actagaacat
     6601 tcatcgtttc accagggatg acgactggtt ttacggcatt tgccaacgca ttgatattaa
     6661 gaacaggtac attttggaat tcagagactt tattcaagtt atagtcattg gttacaatga
     6721 caccatctaa aagcttagcc aatttgatca atttgctgtc tacttcactg atatcttcaa
     6781 agtctccatc gtacatttcg acagatatac cctcttcttt ttgcaaggca tttaggatat
     6841 ctaatccgcg acgaccacgc acacgtttca aactatcccc tgagtccgcg atgtattgta
     6901 gttcgtaaag aacaaaatta gggatcatca aggttccttc taagaatcca gtctttgtaa
     6961 tatcataaat ccgaccgtca atgatcacgc ttgtatccaa gattttatat ttatggaaat
     7021 tttctcctga ttttcgttct aacagttgac tctcaacttg actagctgtt tcttgctcga
     7081 tttttttgct tcgtgaacca aaaactttct tccattcttc gattcgtgtc gttcccattc
     7141 tcaatcccag atagcctaag atgatcatca gtaaaatcgg caatacacta ttgacgaatg
     7201 gaatttccat attgtaaaga ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg
     7261 caccgatcgc accgaagaga agataagtca agctgacctc acttagtttt tgttctactt
     7321 gtttgataat agctgagata tgtttggcca acagcaaaga taataaaaag aaaataagtg
     7381 cgcctaacag gctgttggta aatgtattat tcagccatcc gtttgactgt tgaccgattg
     7441 cctgccaagc tgagggtaaa aatgaaatac ccaagcttgc gccgacaatg atcatcagca
     7501 aagtaatgac acgtttttgc atcgtttttc ctccgtttcg acaatcattt attagttagc
     7561 ggaacacacg cttcaacgtt tcagccaaag ttgctactcc aactatctcg attccttttg
     7621 gcgcttccca gttacctaag ttgtttttag gtaggtatac ttttgtgaag ccgagttttt
     7681 ggacttcttt gactcgttgt tcaatatgac tgacacgtct gatttctcca gtcagtccaa
     7741 tttcaccgat gaaacattcc gttggctgtg ttcctttttc tttatagctt gaagcaatac
     7801 tgacagcgat agctaaatca atcgctggtt catttaattt taccccacct gcagctttta
     7861 agtaagcatc ttgattctga agaagcaacc cagctctttt ttccagcact gccatgatca
     7921 gagatacccg attgaaatca agacctgttg ttgttcgttt ggcatttccg aacatagtag
     7981 gtgtgactaa tgcttggatc tctaccaaaa ttggacgtgt cccttccatt gccacaacga
     8041 tagcagagcc agttgcaccg tctaggcgtt cttctaagaa aatctgtgaa ggattagcga
     8101 cttcttccaa tccatgctca tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc
     8161 ggttttttac tgctcgcaaa atccgaaaac tatgatgttg ttcaccttca aaatataaca
     8221 ccgtatccac catatgttct aacattcttg ggccggcgat cgagccttct ttggtaacat
     8281 gaccgacgat aaaaatggct atcccatttg ttttagcggt tttgagtagt tctgcagtgg
     8341 tttctcttac ttggctgaca cttcctgcca cacttgtgat gtctggctgc gtcatcgttt
     8401 ggatggaatc aatgataaca taatcagggg agatatgttc gatggcacga ctgatttcgt
     8461 tcatatctgt ttcagcgtat agataaaatt ctgtgttgat acttccgagg cgttccgcgc
     8521 gtaattttat ctgctctgcg ctttcttcgc cggatacata taagactttt ccgcctatgg
     8581 ctgctaattg ctgagaaact tgtaacagca aagtggattt cccgatacct ggatcgccac
     8641 caatcaagac catagaccct ggcaccacac ctccgcctaa cacacggttc agttcttcta
     8701 attttgtttt gatccgaggt tcttttttag ggacgacgtc tgcgattttt gttggttttg
     8761 ctttttctcc tgtcaaactt gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt
     8821 cgaccatcgt attccatttt ccgcaatttg ggcagcgtcc caagaactta ggcgacacat
     8881 acccgcaatt ttggcaaaca aattgtactt ttgatttttt tgccattgta ccgcctcctt
     8941 ttatcctaat catgagacat taaatcaagc ttaagtatct ttaacgtttt atttactgga
     9001 agaaccaaat ccgccttgac gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg
     9061 caaaaagatc ccttggccga tacgctcgcc ttttttgatc actacatcct tgaaaccaaa
     9121 gttggtgaat tggaacatga tatggccttc atttgcctca ttattgtaat aatcactgtc
     9181 aatcacacct actccgtttg tcaacactaa aaaccgcttc aacggattgc ttgaacggtt
     9241 agcaagctgc aaaaattcat cttctcccat gtaagctttg attccagtag gcacgagggt
     9301 tggtttcaat tgtttttcat tttctgatac atcttttttc aatccatctg cgactgtttc
     9361 caaccccttt ttccagcctg ctttccagat acttggaatc acgacctctt ctgctgcttc
     9421 aaaatcatag ccagcagcat gatgtgtagc gcgctgcggt agattgatat tttgatcttt
     9481 atatttagaa ataacttcaa atcctctttt tctcaccggt tttcctccct aaataaacta
     9541 ctcctattct aacatatccg aggctgtaaa agactttctt tacaaattct tcaagaaatt
     9601 ctgctatctt aaagtaaacc attaattcgt tctttttcca aacgactgca agttgtacaa
     9661 aaaaactatt ttttacatga ctttactcgt caaaacaagt agaatagaac gaaattaatg
     9721 agtggatatt tgattgaggt gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat
     9781 tgtcaaagta ggaacaagta cattgattta tcccaatgga aatatcaatc ttgcggcaat
     9841 cgatcagtta gcttttgtcc tttctgatct tagaaatcgg ggaaaggaag tgatcttagt
     9901 ctcatcagga gctgtaggcg ttggtatgca taaattacat ttgtcagaac gtccgacttt
     9961 gattcctgaa cagcaagctg cggcggctgt tggtcaagcc gaactgatga atatctataa
    10021 tcaaagattc agtgtatata atcaacagac ggcacagatc ctcgtgacaa gagacgtggt
    10081 agattatcct gaaagtcgta aaaatgtgag gaatacattg gaacagcttt tgattatgga
    10141 cacgatccct atcgtgaacg aaaacgatac tgtagcagtg gatgaattag atcatctgac
    10201 aaaatttggc gataatgatc agctgtcagc catcatggca aaacttgtcg cggcagacct
    10261 tttgatcgtc ctttctgata tcgacgggtt ttattcagat aatccgacta caaatcccga
    10321 ggctcagatg tatcgctata tcgataaaat cgatgaaaag attctggcac aagcaggtgg
    10381 tgctggtagc acatttggaa caggcggtat gcagagtaaa ttaaaagccg cttctaggat
    10441 atttgaagca aacagcgcaa tgattttagc aaatggaaaa aatccggcaa ttatttttga
    10501 tattcttgca ggaaaagaag taggtacttt atttaaggag gacttgaaat gactgacttg
    10561 atgcaattag gacaacaggc aaaagatagc gcgcaacaac tagcacttat gcctacaaac
    10621 cgcaaaaatg agctacttgt tcaaatggca caaaccatca aaaagaatca acaagcgatc
    10681 attgaagcaa atcataaaga cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg
    10741 caagaccgct tgcagctgac acctgagcga atcaatgcca tggctacaga gatcgaaaaa
    10801 attgcttcgt tagacgatcc tattggaaaa gtagatgaga tgtggacgaa caccgacgga
    10861 ttgagaatcg gcaaaaagcg cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt
    10921 ccaaatgtca cgacagatgc agctagtcta gcattcaaat cagggaatgc cgttatcctt
    10981 cgcggtggca aagaagcatt cttctcgaat caattattag tccaattatt gcagcaagta
    11041 ctggtcagtg ctggcgaatc tccttatgcc atccagtttg tcgatgatac ttctcatgaa
    11101 actgcaggac agttgatgcg attgacagaa tacttagatg tgctgattcc tcgaggcgga
    11161 gcaaaattaa tccaacgagt caaagaacag gcaacggtac ctatcattga aacaggtaca
    11221 ggaaataatc acgtatatat cgataaagaa gcacagttat ctatggcagt gaacatcatc
    11281 gtcaatgcaa aagcttcccg tccatctgtc tgtaacgcgg cagaaacatt gctgatccat
    11341 tctgagatcg cacctttttt cttgccagct attgaaaaag aactggtaga acacggggtc
    11401 tctttacgtg cggatgctcg tgcgttagaa tatctagaga cggctgcttt agcagaagac
    11461 acagattggg atactgaata tctagattat atccttgctg tcaaagtggt cgattctttg
    11521 gatgaagcaa ttgcacatat caatcggcat aatactaaac attcagaaac gatcgtgaca
    11581 gatagttatg cggctagtca acgtttctta aatgaagtgg acgcggcggc tgtctatgtc
    11641 aatgcctcca ctcgttttac agacggatcg gtatttggtt ttggtgcaga gattggtatc
    11701 tctactcaaa agttgcatgc ccgtggtcca atggggttaa atgaattaac ttccacaaaa
    11761 tatatcattt atggcgatgg acaaatccgc gaataatgtt tcgtgtgaaa cattattccc
    11821 gccttttgcc ttttttctgt aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa
    11881 attttaatga ttttttttat ttaccttatt tctctgaata tcgctgcttt tctgggagtc
    11941 ggtattttat cccttttctt tcaattcaaa aaacgaagca tagggagtca acgggaaaaa
    12001 tggtcgcagt attttgacaa aatcgggcaa aaagggctgg ttacacgctt gcacattagt
    12061 tacatggttg cattatgctt attagcgaca atcaattact attctttttt cgatcattca
    12121 atcgcttata cgattacgct actgattgct gggatttttc acttatctta taaatatcag
    12181 ctgaataaaa accacttgaa tcggactttc cgctaaaaaa tatccgtacg ataagtagag
    12241 tgccttgaag atcggcaaaa aactgccgga ctttttagag gctgtgacaa agattttgtc
    12301 acagcctctt tttccatata aacggcgttc gcctaccagc tgcctggtaa atctgtttcc
    12361 tcttcgttaa gaagcgaaaa tccgtcaaat gtttcgccat cataataacg aagcagctca
    12421 tccatggaca agttcatcgt tttttctttt tcttcttccg ctgattcagc cagtagctgt
    12481 tcccgataca cctgatacat accagctatt ttttgctgca tcgcttttct agtttcacct
    12541 tccgcattga ttattggcaa ttctttgata tagccacgat aaatcaaccg atcatctgaa
    12601 aattcaatgg gtaattcaac gagtgtcaac ccgacttcct gaaaatattc ttccagcatc
    12661 tattatcccc ccattctttt tcttaaatga attttagctt acctactgaa aattttccag
    12721 tttcattatt ttgagaaaaa aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc
    12781 gaataaatgg tgttcttctg ccaactcctc aatcataagt cacaatattc aacaaacatg
    12841 agaaactgtt ttttgaaatt gttccttgtg attcagagtt aaacgacagt ctcacaacct
    12901 cttggtaaac ggcgttcaat cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat
    12961 gagaagctat ttttccgttt tgtttctcta atacttagga taaaacgctg attttacaac
    13021 ctctgttaaa cggtgttcaa tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata
    13081 tgaggaacta tttttccgtt tgtttctcta atactcagga caaaacgctg attttacaac
    13141 ctcctgataa acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
    13201 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
    13261 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
    13321 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
    13381 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
    13441 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
    13501 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
    13561 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
    13621 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
    13681 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
    13741 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
    13801 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
    13861 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
    13921 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
    13981 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
    14041 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
    14101 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
    14161 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
    14221 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
    14281 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
    14341 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
    14401 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
    14461 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgtc
    14521 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
    14581 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
    14641 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
    14701 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
    14761 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
    14821 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
    14881 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
    14941 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
    15001 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
    15061 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
    15121 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
    15181 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
    15241 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
    15301 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
    15361 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
    15421 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
    15481 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
    15541 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
    15601 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
    15661 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
    15721 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
    15781 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
    15841 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
    15901 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
    15961 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
    16021 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
    16081 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
    16141 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
    16201 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
    16261 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
    16321 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
    16381 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
    16441 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
    16501 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatcca
    16561 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
    16621 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
    16681 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
    16741 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
    16801 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
    16861 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
    16921 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
    16981 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
    17041 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
    17101 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
    17161 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
    17221 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
    17281 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
    17341 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
    17401 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
    17461 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
    17521 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
    17581 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
    17641 catagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
    17701 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
    17761 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
    17821 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
    17881 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
    17941 catcttccct gattcgtaag cttggttgta gatttcttcg ccatattcgt aacattgcat
    18001 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
    18061 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttt
    18121 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
    18181 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
    18241 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
    18301 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
    18361 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgcttg attttcttct
    18421 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
    18481 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
    18541 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
    18601 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
    18661 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
    18721 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
    18781 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
    18841 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
    18901 ttgattctta tcaaactata gaagatagct cttctgcagt agataatttt acaccgccaa
    18961 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
    19021 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
    19081 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
    19141 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
    19201 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
    19261 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
    19321 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
    19381 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
    19441 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
    19501 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
    19561 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
    19621 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
    19681 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
    19741 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    19801 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    19861 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    19921 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    19981 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    20041 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    20101 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    20161 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    20221 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    20281 ttgtattttc tgagacagta gctgactgca aagtactttc tgtatcgttt tcaattcctg
    20341 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    20401 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    20461 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    20521 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttaata aatttgcttt
    20581 ttctgcataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    20641 tcgtttgaat aactatttaa caagtaattc tctctggata ttatccgtta tggattatac
    20701 ttcctgtgtc gtcggacgga taatcatgtt gctgacagac atccggtctg gcgtttctat
    20761 cgcaaatgct acagcagaag ctatatcttc agatgttaat ccccattctt tttgcgcttc
    20821 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    20881 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    20941 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    21001 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    21061 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    21121 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    21181 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    21241 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    21301 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    21361 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    21421 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    21481 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    21541 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    21601 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    21661 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
    21721 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    21781 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    21841 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    21901 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    21961 tgctaaaggc tcttcgtttt ccgcttggat ctgatggatc acttcaaaga gcggatccgt
    22021 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    22081 ttttatcctt actttataac ggacggctta tgatgtctaa tacttataaa acatacttaa
    22141 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    22201 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    22261 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    22321 acttggtgtg acacttttca ctcgaggctc ccgagagata cagttgacgg attctggtcg
    22381 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    22441 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    22501 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    22561 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    22621 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    22681 agatcgttgg ggacttttga tacatcggaa tcatccttta gcaaagcaaa cagtcatcca
    22741 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    22801 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    22861 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    22921 gatcatcaat acttatggga cggaaaaagt attcatccca ctcgatccgc cattacaagc
    22981 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    23041 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    23101 aacaacgaat aagttaagca aaagatccaa tgattaaata acataagttt cttaatttgt
    23161 tccttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    23221 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    23281 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    23341 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    23401 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    23461 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    23521 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    23581 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    23641 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    23701 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    23761 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    23821 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    23881 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    23941 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    24001 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    24061 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    24121 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    24181 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    24241 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    24301 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    24361 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    24421 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    24481 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    24541 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    24601 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    24661 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    24721 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    24781 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    24841 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    24901 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    24961 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    25021 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    25081 acaaagcaaa aacagcttcc ttgatgttag cagcagcttt tgcttcattc tttactggtg
    25141 taacagaacc acttgaattc tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    25201 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    25261 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    25321 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    25381 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    25441 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    25501 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    25561 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    25621 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    25681 ttgcagatga gatggcaaaa ctacatggtg gtgctgcaac acgacctgca caaacaaaca
    25741 ccgtggttaa aacagaaaca tttgccggtg gaacgatcga acaagatatt tacgctatcg
    25801 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    25861 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    25921 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    25981 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    26041 atgtaaaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    26101 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    26161 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    26221 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    26281 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    26341 actccgcggt aataagtcca gccaaacgta acatatgaag agctattttt tatttgactc
    26401 ttcttattac ttgatgcaga acagcttttt tacaacctct gataaacggt gttcaaaaac
    26461 cagtttatcg gtatgaagtc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt
    26521 tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc
    26581 caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa
    26641 atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat
    26701 gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact
    26761 tgcttcaata atttctaact ttttacctgt cgcacgctct aatgccttgg ctaattcttc
    26821 atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac
    26881 ttttgctgca gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc
    26941 agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac
    27001 ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt
    27061 gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata
    27121 agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg
    27181 acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt
    27241 cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc
    27301 ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt
    27361 taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat
    27421 atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac
    27481 acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac
    27541 tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt
    27601 ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag
    27661 caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg
    27721 gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc
    27781 gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg
    27841 tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg
    27901 cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg
    27961 aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac
    28021 tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca
    28081 aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga
    28141 cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa
    28201 tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg
    28261 acctctatat tatcatggtt tattgactac cggtatccag atttctccca tatatttccc
    28321 gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac
    28381 ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga
    28441 ttctgctaaa gcgattacaa caagatacgt tccttttggg aatgggatga ttcgggtagt
    28501 gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg
    28561 ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc
    28621 attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt
    28681 attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat
    28741 cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt
    28801 aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt
    28861 ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt
    28921 tgtaaaagag atctcgggag cttcgactaa ctcataatct gaagaaggaa gccattctgc
    28981 atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat
    29041 ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt
    29101 cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggg ctccatcaaa
    29161 tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc
    29221 cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa
    29281 tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg
    29341 aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt
    29401 ttgtatgccg tatctgctga tttctgaagg cagatatcct gaccaatcac gaaaagctct
    29461 tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc
    29521 atctaggaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga
    29581 catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc
    29641 ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg
    29701 gttcagatct ttaagcatat ttatcttcct ccttgcttgc ctttttatca tattatcaaa
    29761 gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg
    29821 gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc
    29881 tatgttttac ctttagaaaa ataaattttg gggaagacac attctaagta aacaaagcgg
    29941 taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg
    30001 ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca tcatacctct
    30061 tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg tcagataaat
    30121 aaaaaacaaa aaaagttcca tctttaaaac acgctattct tttacttttt ctcacattct
    30181 ctaagaaaag aaaaggagaa tgaatttttg acaattcgag caaaagtgat tatcaaatat
    30241 gtaaaatcag ctactttttt cttaaacgtt tctttatact aaatataaat aagtgtttca
    30301 tgaaaggaga actagatgaa taattggctt gagtattttc ctgaaaacgt tttggaacgc
    30361 ggttacagct atcatctgca cggttttgtc cgtcatttga actatacaag caagtacttg
    30421 tccgcaacag tttctggcac ggaagattat aaggtagtga tcacttggga tgaaaagacc
    30481 aacatgactt gtgattgtct atatgccata gaaggaaaaa aatgcaaaca catggctgct
    30541 gtcttattcg cttacgaaga acgtccaata aaaaaatcca actactcact ttccgaactc
    30601 tcgtcccttg tttcctccgc tagttcctct cttgtacggg agcttttaac agagattctt
    30661 atagaacatc cgcaatttat cgaacgcttc aaagttaaga tgccatttca tgcaataaat
    30721 tattcagata aattaacaac tatcatccat aaatacgatc atattatcaa gaaaaacaaa
    30781 aacaggaaaa cagcgaagtt catcatggag atgcgaaagt tcatccaaga agcagttgaa
    30841 tcgctcattc agcaaaatgc ctatcttccc gcattcgaat tgatcaatga agtgattgct
    30901 actctcgaaa cattctattg ggagcctgag gatgaacgaa cccttcttct gatagaggac
    30961 tgttattatt tatggaaaga attattagca gaagcacctc atgcagaaaa aaggcaaatg
    31021 ttctcttggt tcgtctgcca agtcgatcat actgacgcct cttattcaaa acgctactcc
    31081 ataaaaatcc tgaaagaaga ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata
    31141 gataaaaaaa ctaaagaaac ggtaaagaaa gacgatttca atgaaaaata gcaagtaaac
    31201 gataacaacc atcctaaatc cacaagaaat acagcagacc ttgctttaaa aacacgaatc
    31261 atgcaaagga tgaagtaaag aagacttaac gcgtacccaa tattagtcat ttgactatta
    31321 ggtacgcgtt ttgttttata agtcatgaca aatttccttc gttgctgggt agattttccg
    31381 gcattgttga tagattttat gatacttttc tacattttgt aagagaggtt ccactgcatc
    31441 tgtatattgg ataaaattct gacaacaact ttcaggtgtg tcgaaccact ctaatcttta
    31501 aagataaata ttaaaataac gattaataat attaacagtc tttatttttc accaccaaaa
    31561 acctatcttt gcatatacta aatagacgta tatacacaga taggtttgtc ttgtgaaaaa
    31621 taatgattat ccttaccaaa aaatgtttgt ttttctacat gttcgtactt atcaaaaaat
    31681 atgatcctta ttgaaattac tggctattta tattccaacg acttcttgtg ttaatttttg
    31741 ttatctcata gtaaattatg tttcattttc acttaattta gttgtcacgt gttcgtcaat
    31801 tcgtaaaaac ggtaaatata taaacacact tgcggctata attaatattt gtaaaacagc
    31861 tgctcgccag tctccggctg ttgctaaaaa tcccgatata actggtggcg tagtccaagg
    31921 aatttgcact actacatgat taattaatcc cacagcagtt gcaaaatatg ctactgtcaa
    31981 agaaaaaatc ggtgcaagca caaagggaat aatcagactg atattaaata caataggcaa
    32041 tccaaaaata ataggttcgc ttatattgaa tatagcggga gttaccgata atttagcaaa
    32101 gtccttataa tcttttctac gactgaagat aaatattgca atcaataaag caatcgtatt
    32161 tcccgatcca cccattaccg caaaggtatc tttaaaggcc atattgataa tatgtggaat
    32221 ttgctctttg tttgcataag ctgccatatt ttcttgcata ttctgaatca aaaacggatc
    32281 taacaatgaa cctgatatta ctgcttgatg gataccaaat ccaaaaaata gattagcaat
    32341 agatgtaatt aacaaaaatc ctggtaaacc agttgtaatt ttacttaatg gctctgtaac
    32401 tatcgtggta attagcttat ttatatccat attaaacaat tgaatgatta taaatgacaa
    32461 taaagcaaac gaggtaatcg taataatgat tgggattaat acattaaaag actttaaaac
    32521 agcgggagga atatttccgg atagctgaat ctgtagtttc ttattattag aaagtttaat
    32581 aaataattct gtagccgcta atccaacaaa tatacctaca aacattcctg aagctcctgt
    32641 ataggatata ggtatcacac ccgtcacttc aacagcttta tccgcatttt caggagtaaa
    32701 catcatctgc ataggtgtta atacaacaaa tgtcgaaata gcaactaata ccgctgcaat
    32761 atgatttgta taatttctgc ttgcacacat atgataagat actgctgcta caactaataa
    32821 tgtagttatg cttaatgtac cattggcaat tgaagtacca atttgttgta acgtggtcaa
    32881 tgtactagta tcgacaattt cccccatgaa tcctgttggt tctaagatca ctgaattaat
    32941 aagtgtcaca aatcctgcta acatcataaa tggcataaat gtagcaaacg catctcgcaa
    33001 agttcttaaa tggatctgat tccccaactt agtcgcaact ataccaaatt tttcaaatac
    33061 aatgtccttc ctgctacttt tgcccatatt ttttctcctt tatgcttatt gtaatcgatt
    33121 actttttcca cccaaataat acatcactta tcaaaaaaag taaatacttt taattaaata
    33181 tatatatctt ttttagtata aaaaaataag ctctgaaaaa ataattttcc cgagcttatt
    33241 tttcaacaaa aacagcttca aaaacaaagt cttgatataa atgtctcgat accgaatact
    33301 cgaaaggttt accagacgat agagcaacaa ctttgtctat ctcaatcata aattcacctg
    33361 gatctatttt taatattttt gcgtcaaatt cattcgcagt atctcctttg atacgtatgt
    33421 gcgaagtctt tatttccaga cccagttcat ttgtgatata agcatataca gatttcttta
    33481 aatgttttgg ttccaagcct ggtatgactg ctaatggcat gaatgtttgc tcaatcgagt
    33541 atggttgaca atcaataaaa cggcttcgta caatattata tacatagtct tttacactga
    33601 tacttaattt atcagctact tttttcgatg gaagttcaat tgagaaagta tggatttctg
    33661 aatctacgtg ttcttcacca accacgctaa acaaaccttc gtttggacca agaactcgtg
    33721 agttatcaat tggtgttctg acgacttcag tcccataccc acttctactt cgcaaaactc
    33781 cttcggaaac aagtaagtct acgcctttct ttactgttaa cttgcttgta tgataaataa
    33841 ctgccaattc atctcctgta ggaagtttct caccaatttt atactctcca tttacaattt
    33901 ttttctttat atctaagtaa atttccatat acttcgcaat ttttttcaat ggtgaccctc
    33961 caatcaaaaa catatcttca tctatacttt aacataaata aaccgataaa atccacatca
    34021 aaattataca cacaataaaa aagatatata tatttaaaca aaactattta ctttaagttg
    34081 taagcggtgt tatcattaag tggaaacagg aggtaaaaaa attatgacaa ctatgaaaaa
    34141 gctaactttt ccagatgatt tttggtgggg ttccgcttgg tcagctgagc aagcagaagg
    34201 aaaaggagaa acaggaaaag cagagactat ctgggatcgc tggtttaaag aacaacctta
    34261 ccggttttat caacgaatcg attcctctat tacaaccgat cacatccatc aatataaaga
    34321 agatatagaa ctaatgaaaa aaactgggca taactccttt cgcgtttcta tttcttgggc
    34381 acgaatgttt ccaaatggcg gtaccggaaa agtaaatccc aaagccattt ctttctatcg
    34441 aaaattattt tccgaaatga ataataacgg aatcaaagta tttgccaact tatatcattt
    34501 tgacatgcct gctactcttc aagataaagg aggatgggaa tcacgtgaag tagttgatgc
    34561 ttatgttcat tacgctgaaa cttgttttaa agaatttggt gacttagttt gtcattggtt
    34621 taccttcaat gaaccacttg ggcctatttt aggtacttat cttgaagatt ttcattatcc
    34681 aaacctcatt gactttaaac gcggagcgca agcagcattc ttcactatac ttgcacacgc
    34741 aaaagcaata caagtattta aagaactagc tcttcctagt aaaataggcg ttattctaaa
    34801 tttgagtcct acttttcctc gtagtcaaaa tcctgctgat gtgaaagcag cagaaatcgc
    34861 tgatttattt tatacgcgga gctttttaga tcctatggtt aaagggaatt tttctaaaaa
    34921 gcttatcctg ttattacaag aatataatca gcttcctgaa gattatacga aagaagatct
    34981 tgtatgtatc tacgaaaata ccgctcaaat tcttggacta aactattacg agccacgtcg
    35041 cgtcaaatct cgttcttcta tggtggatac atctagtcca tttttaccag agtggttttt
    35101 cgaaccacat atcatgcctg gacgacgaat gaacgaatac cggggatggg aaatttacga
    35161 aaaaggaatt tatgatttgt gcatggatat caagaaccat tatggaaaca tcgaagcatt
    35221 tatctcggag aacggaatgg gcgttgcgga tgaagaacgt ttcatggatg atacagggca
    35281 aataattgat gattatcgga ttgagtatat caaagatcat cttgcttact tatggaaggc
    35341 aataaatgaa ggatgtaaca ttaaggggta tcatctttgg acatttattg attgctggtc
    35401 atggatcaac gcctataaaa accgatatgg gctggtttca ctcgacctta ctactcaaaa
    35461 aagaacaatt aaaaaaagtg gcgaatttta caagcaatta agtgatacaa atggatttag
    35521 atacgatgta gacttgttag ttgaataata ttctaaaatt ccacttgaag tgaacacatt
    35581 agaaaacaag aataatgaga aataccaata acaaatactt tttcttcata cttcatttat
    35641 acttgtcagt ataagagact gtgacaaatg tagtgatcct cagaagttag gtctggaaat
    35701 ctaacttgag aagatttttt taaactacgt ccagtctttt ttatttgttt aaaattgaag
    35761 ttgacaagtc ttcattaaaa atttagaata ttcacaagtt tgacgacaat tatgaaaatt
    35821 tatgtcgtgc taaaggagtt ttgttattgg atgaaaagtt agatataaaa actgcgatca
    35881 aagacacgtt acctaccgtt tttggttata tcggtattgg acttgcattt ggtatcgttg
    35941 ggaaagctgc cgggtttcat ccgctggtcg tcacgttgat gtccctgctg atctatgctg
    36001 gttctgccca atttatcacg gtcagcatgc ttgctagcca tagtccactg ctctccatcg
    36061 ttctctcgac ctttctagtc aattcccgaa tgattctgat gagtatgacg attgctcctt
    36121 atttcaagaa aaatcggctg cttcaaaatc tgctgattgg cacattgctg acagatgaaa
    36181 gtttcgcact gggaatgaat aaattgaact atactggaca aaagttaaac tttcgttgga
    36241 tgaatacagc taattggatt tcttatttga cgtgggtggg ttcttctctt gttggcgcac
    36301 ttttagggaa ttttattact gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta
    36361 tgtttatcgg gttactttat cttcaagtca tttctgatag aaacacctct aaacgattac
    36421 agttgatcct gattggtctc actttgattc tggtatatgt cggactgatt tttatcccaa
    36481 gtaacttagt gattttagtt gtaacactga ttgggtgcgg attgggggtg tggatcaaac
    36541 atgccttctt ttgagtttac attgctgact attttaggtt gtactttagc aacttggctt
    36601 tctaggatcc taccatttgt cttactgaaa aaattcgatt tgccgcaacc gttgcttgag
    36661 tatttaagtt ttgtcccgat cgtcattatg tctgctttat ggttcagcag tctatttacc
    36721 caaaatatcg gacatctgcc gcagatcaat ctcgaaaatg ctttggcttc agttcctaca
    36781 cttttagctg ctattctttc aaaaagctta ttggtgatcg tcttggtagg aattctttcc
    36841 ctttcgttga ttcgcttatt ataaataaaa aaacaagagc ttatggcaaa ttactgtcat
    36901 gcgctcttgt ttttatcaaa gtcaaataag aaaaaaatcg gcgtattctt gcttatccgt
    36961 tgaacggacc actaacaacg acttttccta atatatgtcc tgtttctacg tctttggttg
    37021 cttgtttcaa gttcttcgca ttcatcccat ctgaatagac cttgctcaat gtactgcgga
    37081 taatcccttc atttgctaaa gcagcaatgt gcgctaaagc ttctccttgc gtttcgattt
    37141 catagttata gtctgtttta gcgaacatat actcccagtc gaagctgata gattggtttt
    37201 tccaaacacc tatatctaac ggctctttga tccctacgat cgttcccaca tgtccaaatg
    37261 gtcggacaag gtgtttgatt tgatcaaagt agcgggtaat atcgaataat acagcgacat
    37321 actctacttg atcgattcct aattcacgaa aacttttact taagtcgtga tgataatcga
    37381 ttggatagtc tacacctgtt tttttcagcc attcaaaatt cttcggactt gcagtcgcgt
    37441 agacttctag gccagcccaa tgcgctagtt gattcaaaat cgaaccgacg cccccagctc
    37501 cgttgatcac taagattttt ttcccgcggt tggcattttc ttctggagtc agaccaaatt
    37561 tttcaaataa caattcataa gcggtcaatg aggtcaacgg caaggctgcg gcttcttcat
    37621 ctgaaagatt ttttggtgca agagcgacaa tacgttcgtc aaccagttgg tattcttgat
    37681 tgcttcctgc tcttgtcgtt gtacctgcgt aaaaaacgcg atcgccgaca gaaaattttt
    37741 gcacttcatc acctacagca acaactttcc cgactccatc aaaccctaag atacgtaaag
    37801 catttcttat cccgttatct tggcgaagtt tcgtatcgac aggattaaca gatactgcgt
    37861 tgacttttac cagaagatcc cgtgaactgg gtacaggtat ctgttctttt tcatcaataa
    37921 agctttttgg atcatcgatc gacaaccctt cataaaaacc gactaccttc attaattcct
    37981 tttctgacat acgaattcct ccttagcttc atcaagttgg agcatccata cagttttcat
    38041 ttcaatatat atagtatgct atttgttcca cttttattca tcataattat tctcttactt
    38101 aaagtaaact atttgcactt ttttggttaa aaaagaggtt gcgacaagac ttttgtcaca
    38161 acctctttta aacggtgttc aatcaactga ccctcggtat tcgctcaaaa aacgacaaaa
    38221 tatgaggagc tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcacg
    38281 acctctaatt cacggtgttc aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc
    38341 tttttcaaag gtgtctaaga tctctcgtac ttcatcagta gatttcgtat tcatcaactg
    38401 aactcgcaag tcatttgctc ctggaaatcc tttgatatag attttaaaga aacgatgtag
    38461 tcctgtgatg gaacgcggga tgatttctga ataatgatct tgcagatcta gttgcagacg
    38521 aagtagtccg atcagttctt tttctgtatg ttccctcggt tctttttcaa atgcataagg
    38581 attcttgaaa atcccccggc caatcatgat gccatctacg ccatattttt cggccaattc
    38641 ttctcctgtc tggcgatctg ggatatctcc gttgatcgtg atcagtgttt gcggagcgat
    38701 ttggtcacgt aaagcaatga ttttagggat gacttcccaa tgtgcatcta ctttactcat
    38761 ctctttacgc gtacgcaaat ggacagaaag attggctata tcttgatgaa gtaggtgact
    38821 gatccattct tccatctcag acatttccgt atagccgata cgcgtcttga cgctgacagg
    38881 caaaccgcca gctttagccg cttcaatcaa ttgtgccgct acttctgggc gaaggatcaa
    38941 gccactgcct tttccacgtt ctgctacatt agggactggg cagcccatgt tgatatcgat
    39001 tccttggaag cccatttctg ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc
    39061 tccccaaata tgggcaacca tcggctgttc atcttctgta aaggtcaatc ttcctcgtac
    39121 actgtctttt ccatctggat gacagaaact gtctgagttt gtaaactcag taaagaagac
    39181 atccggtgct gctgcatgct ttacgacatg gcgaaataca acatctgtta catcttccat
    39241 tggtgctaaa acaaaaaaag gctttggcag ctctttccaa aaattagtag tcatttattt
    39301 gttactctcc attctatcaa gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat
    39361 actaggaaac aaaaaaagaa gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa
    39421 aaaagactgt gacaaaaatc ttatcacaat tccttttccg gaataaatgg tgttcaaaag
    39481 cttgcatctg cggtaataag tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg
    39541 ctgttcttat tacttgatgc agaacagctt tttcacaacc tctccactaa acggtgttca
    39601 atcagctgac cctcggtatt agctcaacaa acgacaaaat atgaggaact attttcccgt
    39661 ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttttct aacttctcaa
    39721 tcctcttcct ttattgatca aataccaagt aacggcgtac aaaacaacga tgaatagcac
    39781 aagtacgatc atcgatgttg ctactggcac atcgattgtt ccaaggaatc cgtaacggaa
    39841 accggagatc atataaacga tcggattgac tttcgaaaca gcttgccaaa atggcggcaa
    39901 catagagatg gcatagaaaa cgccacctag ataagttaac ggctgcaaga caaatgtagg
    39961 cacgatcgat acatcgtcat aagactgagc aaaaatcccg ttgagcagtc ctgctaacga
    40021 aaacaagatt gccgtcatca ataatgtaat gatgaccatg aaccatgaat acacatgaag
    40081 tggtacaaag aacaaggaaa tgatcgttac gagagagccg acgagaatac tgcgacccac
    40141 tccgccaatc acaaagcccc aaatgatcac atgcgtagga acaggcgcta ccaagatttc
    40201 ttcgatgttt ttctggaatt tttgagagaa gaaggacgaa gaaacgttgg catacgaact
    40261 agtgatagca gacatcataa tcaaaccagg cacgatgaat tccatatatg aaaaaccacc
    40321 catgtcaccg atccgaccac cgatcatttt tccgaaaata acaaagtaca aggatgtcgt
    40381 gatcacaggc ggaaccagtg tttggaccca gatacgcaaa tagcggttcg tttccttgat
    40441 agctaaactt tttaatgcag taaaataaag attaaacatt tttctcctcc gtatgattat
    40501 tttcttctgt gattttcaaa aacagttctt ctaaacgatt cgacttattc ctcatagaaa
    40561 gaactttgat tccttgtctt gtcagctgat cgaagattcc gttcacccct tgatttcgtt
    40621 caacttcgac agcgattgtc tgttcatctt caaatgtaca agcgtatcct tcaatcacag
    40681 gtttggtctc atatggcgct aaatcaaaga taaatgtttc aaattgcagt ttggatagaa
    40741 gctctttcat actagtattc tcgatcagtt cacctgattg gatgatcccg atgttgcgac
    40801 aaagcatttc agcttcttct aaataatgcg tcgtcaaaat gatcgttgta ccattttcgt
    40861 tcaattctcg gagaaactcc cacatctcgc gtctcaattc aatatcgact cctgctgttg
    40921 gttcatccaa gatcagcaaa cgtggttcat gcatcaatgc cctagcaatc atcagtcttc
    40981 gcttcattcc cccggaaagc atacgtgcac ggacatttct cttttcccaa agattggact
    41041 gcttcaaata tttttcgctg cgtttcatcg cttctttgcg agagacaccg tagtagcctg
    41101 cttgattcac gacgatttgc tggaccgttt caaagggatt gaaattgaac tcttgcggga
    41161 ctaatccaat ctgttgcttt gctcggacta gatccgtatc cagatcataa ccgaagacat
    41221 tcacttttcc agatgtttta ttgactaaag aagtaatgat tccgatcgtt gtagacttcc
    41281 cagctccatt tggtcctaat agcgcataga aatctccttc tttcacggaa agatcgattc
    41341 ctcgcaatgc ttcgacccca cttccataaa cctttttcaa attatttatt tcaagcgcat
    41401 ttgccattta tttcctatct ccttatagac atgattcttt attgtatcat ggaatacagc
    41461 agatgaaaaa acaacttggt tccagcgaaa aatcaggttg ctgggctcag gaaaatctcc
    41521 taatgaaaga catcaatcgg acgaagtcag acaacctttt cagaaaaaac aaatcgaaga
    41581 taaaggatta ctattcatat gtgcaacaaa aggagcgtga gacaaaagta aagaacactt
    41641 ttgtctcacg ctctaaacac gtataaacgg cggaagcaaa agccaagatt tttgatccaa
    41701 taaatacttt cgatcacttc gcctatccca atacatgcca ctgccgcttt tcatcaaata
    41761 acgcggcttc cgtttttgcg gtttctacta aagtctctgg gtaatccaaa actttcagca
    41821 aggcaatcgc atttcgggtc cttgaaggtc cttcttttaa tttgaaatca aaactgattc
    41881 cttttccttc tgttacttgt tcttcaaagt ggacattctc acaataattc tttaaaatct
    41941 ctgtcaattc gatatcatgt gtggcgacaa atgctaaaga tggatactcc gcaagccacc
    42001 gaacgactga agcggaagaa gcaatccgtt cgatcgtatt tgtccctttt aaaatctcat
    42061 ctacgaagca gtagcagcgt tctttggaag ccacttggtc caacaaacgt ttgatcgatt
    42121 tgatttctga aacaaaataa ctgtcgcctt caaataaatc atcttcgatt gccatggaag
    42181 tcaacacatg tccaggttgc agggtaaact gttctgcaat cgctgtttgg atcgtctgtg
    42241 ctagaataca gctgatggct atacttttga catacgtaga ttttcctgat gcattggaac
    42301 ccgtgactaa tgtattcttc tgccagttga ccggattcac taccgcttct tttaacaatg
    42361 gatgatagat atctttcgct gtcacaccgc cctcagtaaa ctctggtcgg caagtgatgg
    42421 gcatataagt acggtaattc aatattgcag cggctacttc taattctccc agcaattccc
    42481 acagcttgat ggcttcagtt gaatgctttt ctaatttagt gagaacgaaa tgataggaga
    42541 taaatggcag catgaacatg atgttgacat attcaaaaat caactctgct tcactcccac
    42601 tcttcatacg aaatgaaaaa cctaatttag ggatgtctct taatggttta gtttgctgtt
    42661 tcagctcttc ttgaagtggt gtcggcagtt ttgctagctg attggcggct gaaatcgttg
    42721 aaaccaaata actcatgcta ttcagctctg tttctagtgt ttgtttcttc actagataat
    42781 agaccgtatt gaatactaac ccaccgattg ccaaatagat tcctagtact tgtcctaaaa
    42841 gaagcaaacc caaacccacc agcggaagca agccaagaaa aataaataaa cctacgcgtc
    42901 caatcttttt actttttcca tccgctagat attgttttgc aaaattatga tctttttttc
    42961 ctaaacgagc gaattgatat tggatttttt ctctcagttg aggattttcc tgataaaaag
    43021 caatcagttt ttctaactgc tgatcttcac caaaatcaaa gctgcgtaaa cgttgataca
    43081 acgcttctga acctacacta gaataggtac tgttgattcc ttcgaatact tcgaacatat
    43141 ccaggtcata ccaagtaaga tcatcgattt cgctatccca tgaacggaac ttcttctccg
    43201 tgagccatgc ttccttcaag ctttcttctt tatcaaatct cggttgataa ggaatttttt
    43261 cccatttgct acgaactgtt tcctttaatt tcaaacgtga aaaaatgtct aaagcaacta
    43321 ataaaataac agctgcaata ataattaaaa cgataaattc tctttgaccc atatccctct
    43381 ccgttccttt atttgttagc tttagtatag cactgttgcg ctatttgaaa agagggtaaa
    43441 aaaacacctt tcaaaataaa aggtgttttt ttcttgtata ttacatattt atctctcatt
    43501 tatcaaaatt gacaaataat catagggagg aattgttgtt aattcttgtt tattctccag
    43561 tgcattgaca actgtcccaa gggaaaggat agcctcaggt actatttttt cttgttctgt
    43621 tggattagct aagcgaagaa caaatccttg ttcatgaaaa taagaaggat aaatcgatga
    43681 tacaattaag tcttcaggta attcaatcaa tgaaaattta cgaggaacat caagttcatt
    43741 ttgtaatggc caaatcttgt tatctaacct gtgaataaat acattcagtt tctgcttttg
    43801 ataagaaata gattgttcta accgtttttc tgcctgtttt gccactttga actcattaaa
    43861 tcttccttct tctacattaa atccaaaagt gactttatac tcacctatct cttgagctag
    43921 cggtgtttcc atcatgacat gcccttcatt tgtcgtatct cctgaagctc gcccaggtcg
    43981 ccaagcaaga ttaggttttc caagttcccc tgttgttgaa aataaggtta tgattaattg
    44041 attttctata cgttcatact cttttaaacc atctgcataa aacgtaaaat aatgatcatt
    44101 ttctgccaca gagacactct tatcgaatat ttctaaatta acaggtttct ctaccatagt
    44161 ttctgaccaa ttcggaggaa tgacatcagg ggtattttca ataaatccgt tttgaatttg
    44221 tgcaattgat ttttcagatt tatctaaaac attaattgct aagcgcatac gatgcgagta
    44281 aacagtatta tgaattttga gtgtgccttc aacgatttga ctttctccca gactgaacga
    44341 tatttcataa gatatttctt ccatttcggg tttttcagat aaacgatctt ttaaatcttt
    44401 aggaagttga gcttttccgt aaacaactaa tgtttcttgt aaagaatctt tatatacttt
    44461 agctgtttct aattttagag atagttctgt atctccttct aatggtgaat aatcatacgt
    44521 atcgccatca tttgctgaat cgatcaaatg aacgaaatca agatattgtc tatctccaac
    44581 tatcaaattc aattgcccat ctttaaaaga aagtttatat ttattgttac ttatctcagt
    44641 tttgtctatc tcgattgctg tttctaattt ttcattattt ttctcaaatt caacaacagt
    44701 gtatcctagt gctggcacat ttacatttat ttttatgtca agttcataat aagctggttc
    44761 atcaaaatat tcgaaacctg tagcaatcat ccgataagca tttttccgtt ctggaaaata
    44821 aacttctttt tcaatgatat tatagggact acctttgaag tgtattttct ttgaatcagc
    44881 tactacatgc acgattttcg atccactata attctcagtc gttgtgttga aaacaaggac
    44941 ttcattcgat tttagcttta atgcatcagc catccgtttt acaataaggt tttcgattcc
    45001 gtcagctatc tcattagctt ctttgattcg atggaaaata tcttccgcaa cattatctga
    45061 aacacagcca ccaatactat catgagcctg gttttgcaaa atctttttcc ataaccgacg
    45121 aagtaggcct ttactgatat ggataccatt tctttctgca atcagcatga gcggctcaac
    45181 tcgttttaga attttttgtt ccaatttaaa attttctatt ttcatttttg ttcttactga
    45241 gccaatagaa cgatgaacac gagagtaaac cggctgacga agttcacctt tatattcagg
    45301 taattctttt ttgtttctga taacttctac ataagaagga taatctgaaa taatattgtg
    45361 gaatttactg tatttattca acttctctac gacttgatca aaatttaaaa tcattgactt
    45421 ttgatctaca cccgaaggta ctaaaatatc tttgtttttt ccttgcatat gaagcatttc
    45481 aactgcttca tccaactttt cttttgcaaa ttcaggaata tctttaattg cgtctaaggt
    45541 catcattcca gtcatataac caaatggaaa attcatggca taaacaaatt tatctcccaa
    45601 tcctttccag ataaaataag gagatggaac catcgtttca aagtttaatc ctcgccaaca
    45661 catgaaatta tcgatttcta cttgatgcaa aagtgtaggc aaattcgcat tgaacccaaa
    45721 cgtgtcaggc aaatacccta ccatcattgg atctccatat ttaaggatcg tatcactgat
    45781 tccaatcatt aagtttctta aaattgattc agcatccact aataaggcat ctgtttgtgc
    45841 ataccacggt cccacgaata gttgtcctgt tttcatcagt cgttggataa ccttaagctt
    45901 ttgcggattg atctccaaat actcatcgac gattgaagtt tgtccatcta aacaaaagtt
    45961 tactttaggg ttcctttcca attcatctag aacttcagta aagacctgat cagacaatac
    46021 gatggcatct tcatttgtaa aataccattc tctgtcccag tgcgtgtgtt gaattatatg
    46081 tgcatttacc atatcagatt ctcctacttt ccttcttcta ctgaatttac ttgttgttct
    46141 tttttatact tgatggattt tagtgcattg accattaacg ctgtaaccac tcccccaata
    46201 acaattgata ctaaatataa aggtatattc gtaacaaaag gaattgccat aaaggttgaa
    46261 agcattgttt cttgagtaac accaaaaaat ccatttagac cacatgctac tgcagtgccc
    46321 accacgatgc ttggtattac tcgtaaagga tctttcacgg caaacgggat cgtcccttca
    46381 gtaatcccca caaggcccat aatcatagct gtctttgcat tatctcgttc cgattcatca
    46441 taaagttttt tgtttaaaat tgttgctaaa aacattccaa atggcggaat agcaattcct
    46501 aataacatgg ctgtactagg tccaaagctt ccgcttgaaa aagaagcaac cccaaaagca
    46561 tatgcgattt tattgatagg tcctcccata tcaaacgcca tcattcctcc taaaatagcc
    46621 gacatcaaag cagcatttgc tcctgacatg ccttctagcc aattagtcag tcctgttgtc
    46681 attgcttgaa taggcgtagc tagtacatac cataatatga ttccaacaac taatgtagta
    46741 acaaaaggaa tcagcataat tgattttaga ctaataactt gcatcggtac ttttactttt
    46801 ttcaatgcat tagctaaata gccacaagct aatccagcaa ccattcctcc aataaatccc
    46861 ataccaagat tttgacacat catcccagtg ataaaagctg gtgctagacc gggacggtca
    46921 gcaattgaat atgcgatata ggctgaaatc atcggtacga taaactgtaa tcccgtctga
    46981 ccaatcattc gcaggactcc ccagaatgta tccgtctggt cccaaacacc tgtgccacca
    47041 aaaatgactg caatagaagt tagtaaccct gctgctacga caagcgggat catataggaa
    47101 accccacttt ggaaatgatt tagaatttct cttccttgtt tcttgaattc cattagtaac
    47161 tcccccacga tatatttttt atttgttaat taatgcaatt gcttcatcaa tcgtctcttc
    47221 accatctttt actacctctg aggtccctac tgttaaaatc ggtttaccgt caaagcgacc
    47281 tcgttctgaa acatttgtat ctactgcaaa tatgacaaca tctgcctcgg aaacatccga
    47341 aattgaaagt ttgttttcta tccccatagc cccctgtgtt tctactttaa tatctaaccc
    47401 tttcttcttc gcaaactttt ctaaattagc ttttgccata taagtatggg caatccctgc
    47461 gatacatgct gttactgcta ctatttttgt catatgaaat tcctcctttt aaaatgatag
    47521 agcttttacc aattcttttt tatttctcga atcttttatt cttttaacaa actcgtcatc
    47581 catcaatctt ccagataatt ctgctaaaat acgtaaatga ttagtacctt tatcagtgtc
    47641 ttttattgct aacaaaaaga cgagatcaac tggttgatca tctaatgcgc cccattcaac
    47701 agaccgcttt aacttggcta cagccactgt tgattcaatt actgctaggc ttttgccatg
    47761 aggaatggcg atatttttcc caattcctgt ggtcatatgg ttttctctgt caaatacgtc
    47821 tttttgaaat tgtttgtggt cagacacaaa cccattagtc tctaacaatt tcgataattt
    47881 ttcgactaca ctttttttat cagatgcatt taaatctatc actatattat ttatattgat
    47941 tttttcactg atcatttata agccttcttt caatatataa actaattttt caaacgacga
    48001 actcgttttt aaatttgcta ataactcatg attcgaagcg atttcattga atgtttcata
    48061 aattttattc aattcaggtt ttactttttc attaagtgca aaaaagaaaa cagaatgaac
    48121 aagtgtccca tcccaatcaa ttccttctgg attaacgtag acacagatta ctggcttttt
    48181 tataaaatct acttcgatat gcggcatagc aaaatcattc atcgctgttg aagatagttt
    48241 ttctcttttg attgctccct ctagtatccc ttctttcgcg taattttctt ttaccaattt
    48301 tttggtaatt atatttaaaa cgttcacttg attatccttt atttggtggg taaaaaagca
    48361 aaatcgttta tctaataaat ttagaaaagc atcttctgtt tgttcattta actgtagata
    48421 ctgttttact tttctgacat ctgcccctaa catcaaagga ctaacaacaa taacaggaaa
    48481 atttgtattt tcaatcggaa tcgtgcttat aacaagttca tcagtgactt ttctatcttg
    48541 tagttcattg atgcttagta catcttctat ctcaaccata tctgcaaaac cgttatttat
    48601 tctttgctct aataattttg atgttccata tccgctacta caaactaata tcactttcgt
    48661 tttgtcaggt ttgtaacgat ccaaaagtga ttgaatatgg agtgttacgt aagcgatttc
    48721 atcttcatta aaatgtatac agaatctctc ttcgatttcc tctaataaat ctacacttat
    48781 cataaatgac cttttaaaag aatatttgat tttgtccgta taagggttat agatatttac
    48841 tcccaatttg agtcttttaa ctgctgcatt gagatgtaat actagattat tgatcaattc
    48901 ttcatcagga taaaattcat ttaatttaat tcttactaac tcttgtagct caacacccat
    48961 atctaaatag gaaagcttcg tttgatctaa gtcgtgattc atagcatttt tttcaatcga
    49021 agtaatgtga atgtcaacat aagcttgttc atctattggc acaataattg aaaatgcttt
    49081 ttcaatcatt ttgattaaaa taagtgtatt ctttgagatt tcttcttcag aattattact
    49141 acgtgataag aaattgcttg tttcaatcct ttctatcgca atagcaaggt ggatagctaa
    49201 agtttgaaat tcataatctg taaatttcaa ttgtgtctta ttcatgtatc gattaagcaa
    49261 ttcggctact tttttcaata tgtccttatt taagaaatta ttcgcttcac caatgaaatt
    49321 gatattcaag tctaagagat ctgttttact gttagatgct gtaaagccac cccaataata
    49381 gttcacaact tctgacatca accttctttt acttttttca tcagctttga tgcgcacgcc
    49441 atttcgatct gaaactaagg acatcccatg cttgtttagt ctttttctaa catctttcat
    49501 tgtgctttct accgtcgacc tgctcacgta taattcgtca gcaagatttt gaatctttag
    49561 agaatcatta gtattcaata atttagcaaa cacataagtt agacggtcct ccggagagtc
    49621 tgcgatattg attttatgac tggataataa atttcgaagt gtttcttttt ttccaataag
    49681 cgaaacgcct tttttaggtt gaaccaacat ttgtacatct ggcatttttt ggattatttc
    49741 tttcaaaatt aaaatatctt tgctaattgt tttcgtagat acatataatt gttctgataa
    49801 ttctttgtaa gttgtgtgct cattttcaat caaaaagtat agtattttca tttgccgttc
    49861 gctaaattcg atgcccatta tcttcacctc ctgaatacag cttaagtgta caattattat
    49921 ctctattttc atacagaaga aagttcttga ttaatgtaga agtttctaat attcttgatt
    49981 atttca
//
LOCUS       DAZAFF010000009        45550 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000009 DAZAFF010000000
VERSION     DAZAFF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.009"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..730
                     /locus_tag="ACG9BB_000539"
     CDS             <1..730
                     /locus_tag="ACG9BB_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2836511.1"
                     /translation="LFSSDQAFLITKNTILYNVAFILLNLLISVVFAIIMSELRNKRL
                     VKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFI
                     LVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTIL
                     TILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQS
                     VVGFVLLMITNTIARRFDSDSALF"
     gene            742..1662
                     /locus_tag="ACG9BB_000540"
     CDS             742..1662
                     /locus_tag="ACG9BB_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2836512.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1703..3160
                     /locus_tag="ACG9BB_000541"
     CDS             1703..3160
                     /locus_tag="ACG9BB_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2836513.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3505..4119
                     /locus_tag="ACG9BB_000542"
     CDS             3505..4119
                     /locus_tag="ACG9BB_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN2836514.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4112..5836
                     /locus_tag="ACG9BB_000543"
     CDS             4112..5836
                     /locus_tag="ACG9BB_000543"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN2836515.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5833..7284
                     /locus_tag="ACG9BB_000544"
     CDS             5833..7284
                     /locus_tag="ACG9BB_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN2836516.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7757..9910)
                     /locus_tag="ACG9BB_000545"
     CDS             complement(7757..9910)
                     /locus_tag="ACG9BB_000545"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFN2836517.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9912..10958)
                     /locus_tag="ACG9BB_000546"
     CDS             complement(9912..10958)
                     /locus_tag="ACG9BB_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2836518.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11108..12397
                     /locus_tag="ACG9BB_000547"
     CDS             11108..12397
                     /locus_tag="ACG9BB_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFN2836519.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12410..15112
                     /locus_tag="ACG9BB_000548"
     CDS             12410..15112
                     /locus_tag="ACG9BB_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFN2836520.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            15097..15954
                     /locus_tag="ACG9BB_000549"
     CDS             15097..15954
                     /locus_tag="ACG9BB_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN2836521.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(16025..16882)
                     /locus_tag="ACG9BB_000550"
     CDS             complement(16025..16882)
                     /locus_tag="ACG9BB_000550"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN2836522.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            17093..19264
                     /locus_tag="ACG9BB_000551"
     CDS             17093..19264
                     /locus_tag="ACG9BB_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFN2836523.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19332..19673
                     /locus_tag="ACG9BB_000552"
     CDS             19332..19673
                     /locus_tag="ACG9BB_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN2836524.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19949..21703)
                     /locus_tag="ACG9BB_000553"
     CDS             complement(19949..21703)
                     /locus_tag="ACG9BB_000553"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2836525.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(21714..22550)
                     /locus_tag="ACG9BB_000554"
     CDS             complement(21714..22550)
                     /locus_tag="ACG9BB_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN2836526.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22552..23235)
                     /locus_tag="ACG9BB_000555"
     CDS             complement(22552..23235)
                     /locus_tag="ACG9BB_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFN2836527.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(23394..24788)
                     /gene="uxaC"
                     /locus_tag="ACG9BB_000556"
     CDS             complement(23394..24788)
                     /gene="uxaC"
                     /locus_tag="ACG9BB_000556"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFN2836528.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(24827..26326)
                     /locus_tag="ACG9BB_000557"
     CDS             complement(24827..26326)
                     /locus_tag="ACG9BB_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFN2836529.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(26319..27740)
                     /locus_tag="ACG9BB_000558"
     CDS             complement(26319..27740)
                     /locus_tag="ACG9BB_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFN2836530.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(27800..28792)
                     /locus_tag="ACG9BB_000559"
     CDS             complement(27800..28792)
                     /locus_tag="ACG9BB_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2836531.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            29024..31222
                     /locus_tag="ACG9BB_000560"
     CDS             29024..31222
                     /locus_tag="ACG9BB_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2836532.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(31680..32552)
                     /locus_tag="ACG9BB_000561"
     CDS             complement(31680..32552)
                     /locus_tag="ACG9BB_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2836533.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            32852..33826
                     /locus_tag="ACG9BB_000562"
     CDS             32852..33826
                     /locus_tag="ACG9BB_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2836534.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            33848..34540
                     /locus_tag="ACG9BB_000563"
     CDS             33848..34540
                     /locus_tag="ACG9BB_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFN2836535.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            34506..36014
                     /locus_tag="ACG9BB_000564"
     CDS             34506..36014
                     /locus_tag="ACG9BB_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN2836536.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            36088..37191
                     /locus_tag="ACG9BB_000565"
     CDS             36088..37191
                     /locus_tag="ACG9BB_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFN2836537.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            37194..38531
                     /locus_tag="ACG9BB_000566"
     CDS             37194..38531
                     /locus_tag="ACG9BB_000566"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFN2836538.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            38518..39396
                     /locus_tag="ACG9BB_000567"
     CDS             38518..39396
                     /locus_tag="ACG9BB_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFN2836539.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            39393..40316
                     /locus_tag="ACG9BB_000568"
     CDS             39393..40316
                     /locus_tag="ACG9BB_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFN2836540.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            40313..40723
                     /locus_tag="ACG9BB_000569"
     CDS             40313..40723
                     /locus_tag="ACG9BB_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFN2836541.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            40727..42295
                     /locus_tag="ACG9BB_000570"
     CDS             40727..42295
                     /locus_tag="ACG9BB_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFN2836542.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            42535..43953
                     /locus_tag="ACG9BB_000571"
     CDS             42535..43953
                     /locus_tag="ACG9BB_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2836543.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            43965..45275
                     /locus_tag="ACG9BB_000572"
     CDS             43965..45275
                     /locus_tag="ACG9BB_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFN2836544.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT        14782 a         8019 c         9310 g        13439 t
ORIGIN      
        1 tttattttct tcggatcaag catttctcat cactaaaaat acaattttat ataatgtagc
       61 atttatcctt ttgaatctgc tgatctcagt cgtatttgca atcattatga gcgagttgag
      121 aaataaacga ctcgttaaag tttatcaaac gatgtcctta ctaccttatt ttctatcttg
      181 ggtcattatc agttactttg tttacgcttt tttaagtcct gacaaaggaa tattcaatca
      241 gtggatcact ggacatggag gagaagctgt caattggtat aatgaaccga aatattggcc
      301 gtttatcttg gtgttcattg gtacatggaa agggattggt tataacagca ttatttactt
      361 tgcatctgtt atgggaatcg atccgactta ttatgaagct gctatggttg atggagctag
      421 caaatggcag cagatcaaac atgtgacgat accacaactt attccactaa tgacgatttt
      481 aacgatttta gccgtaggaa acattttccg agctgatttt ggcctgtttt ataacattcc
      541 taggaattcc ggtgcattat atgatgtgac tcaagttttg gatacgtata tctacaatgg
      601 cctgacatcc acaggagatt ttggtatgac ggctgctgca ggactttacc aatcagttgt
      661 cggctttgtc ttgttgatga tcacaaatac gatcgcccga cgttttgata gcgattctgc
      721 attattctaa ggaggaaaaa aatgaaaaaa aagaaagtgg agagagtcca agtccgctct
      781 ttcaataaga cggctaattt tttctctaat ataatgattg cactctttgc tatttcctgt
      841 attttgccat ttatatttgt tatcgccatc tcttttaccg atgagtccat attgtcgact
      901 cacgggtaca gtttttggcc agaaaagttc agtatatttg gttatacgta cttgttcgac
      961 cagatgcagg ataaaatttt ccaagcgctt ttcgtcactg tacttgttac tgttttaggt
     1021 acactgatca atagcacggc tacttcttta tatgcttatg cgatttcacg gtcaaacttt
     1081 ccattccgtc gtttttttac cgttttttgt ttaattacga tgttgttctc accagggatg
     1141 gtggccaatt atcttgttat gacaaatctt cttcagttaa aagatacgat ttgggcattg
     1201 atcttgccaa tggctgtcag tcccttcaat attattgtga tgcgcacatt ctttaaacgt
     1261 tcggtctctg attcgattat tgaatcggca cggatcgatg gagctagcga actgcgaata
     1321 tttgttcaga tcgttttacc gttagctgtt ccaggtattg cgacaatcag tttatttgcc
     1381 gcattgggtt actggaatga ctggttcaat gctttactat acatccaaga tgataagcta
     1441 gttccactac agtatctttt gatgaaaatc caaagtaata ttcaatatct gacacaaaat
     1501 tcaggagcag gaagccaatt gacaggcgga ttagcctcag ttccaggaga atctgccaga
     1561 atggcgatcg tagtcatctc gacgttgcct attgcgatca gctatccatt tttccagaag
     1621 cattttgtca aaggtctaac aatcggcgga gtaaaagaat aaagaaacaa aaatataaat
     1681 cataaaatta ggaggaagaa aaatgaagtt atggaagaaa ctagccttta caggtgtttc
     1741 agcgttgttg ttgggaacat tagcagcatg cggaggttct ggttctaaag accaggcaga
     1801 agctagcaat tcggacactt tgcagatgta tcaaatcgga gataaaccag ataatttcga
     1861 tcaattgatg gaagtagcaa acaaacgaat cgaaaaagag atcggtgtaa aagtcaatat
     1921 caattacatt ggctggggcg attacgaaaa gaaaatgaac gtcatcattt cttccgggga
     1981 aaattatgat atcgcctttg cgaacaacta tgtttccaat gcccaaaaag gagcctttgc
     2041 tgatttgact gaattagcgc ctaagtacgc tgaaaaagct tataatgatt tagatgaagc
     2101 atatattaaa ggaaatctgg tcaatggaaa attatatgct ttcccagtca atggaaatgt
     2161 ctttgctcaa caagtactta cttttaacaa agctttacta gataaatacg atctttcaat
     2221 tgatggaatc caatcttacg ctgatgcaga aaaagtcttg aaggaattcc atgaaaaaga
     2281 accaaacacg gcagcttttg ccattggaca aggcttcaaa gtccaaggga actttgatta
     2341 tcctttaggg aatacgttac catttgctat tgatctggat ggagatgatt cgaaaatcat
     2401 caaccaatac gataatgaaa cgtatatcgg actgttacgt acaatgcata gctggtacaa
     2461 acaaggactc attccaagtg acgctgcaac gagcaataag gattatccac tagaaggaaa
     2521 tacttggcta atgcgtggag aaacacaagg accgtatgat tatggcgata caattttgac
     2581 aaatgcagca aaacaagaac ttgtctcaaa agcaattact gttccactaa aatctacagc
     2641 tcaagcacaa atggcaaatt ttgtcgtatc aaatacttca aaaaataaag aaaaatcagt
     2701 agaacttcta gggctgctta acagtgatcc agaattattg aatgggcttg tttgggggat
     2761 cgagggagaa gcatgggaaa aagtcgatgg ttcagatgat aaaatcaaat tgctagatgg
     2821 ttatcagcca aatacacata tgtctgcttg gaatacagga aacaacaaga ttctatatac
     2881 acaagagtct attacggatg acatgattgc caaacgtgat caatcgattg ctgatgcaga
     2941 aacttcaccg attttaggat tcagcttcaa cactgacagt gtcaaaacag aattaagtaa
     3001 tatttctaat gtcatgaatc aatatttaga tggattgaat acagggactg ttgatccaga
     3061 tgaaacatta ccgaaattga aagacgcttt ggacaaggct ggctatgata aggtattgaa
     3121 agaaatgcaa aaacaatatg atgagttccg acaagaataa taatgaataa agacaaaggg
     3181 gttgtgacaa aagacgtgtc acaacctctt gttccgaata aacggtgttc aaaagccagt
     3241 ttctcggtat taagctaaat atatgtaaaa atgtggaaag ccatattcac aaaatttttc
     3301 ttactactcg aaactaagcg gctatttcac aaactcttgg taaacggtgt tcaatcagct
     3361 aaccctcggt attcgctcac aaaacgacaa aatatgagga actatttttc cgtttgtttc
     3421 tctaataatc aggtcaaggt gctgattgcc caaccttttt ttgtataatg agaaagtata
     3481 aacttaatgt ttggaggaga agatatgtta gggaaggttt tggaagagtt atttatccgg
     3541 atctgggtca ttataaaact aaccttgtat ttctggatat atacatttgc cggcgggatc
     3601 atctttggat taggtgcggc atggaaaaca gtcaatgaac tattttattt gtacggattc
     3661 gagtataaag aaatcacaat aaaaagaggc tggaatatat ataaaagaaa ttttttaaga
     3721 ggcaatctgc tatttagttt atttttatct ggtactgctt tattgagtta caatttatat
     3781 ttatctgttc aaatcaaagg gctcattttt ttagctattg attttatttt actttttgcc
     3841 ttgctctgcc tatttgctac ttatcaatac agtatgattt tagatagtga atatacgatc
     3901 tctgttccta atctgctaaa gctaagtttt atttcggttt tttccagctt ttcgtctttt
     3961 ctaaaaataa ttattggtag cggtataatt ttaggcgtga cttggcaatt caaaggattg
     4021 atcctgtttg gtgtcattgg tcttttgacg gtttggaatg gtacgatgac aacacattgg
     4081 cgtgaagaat tagataaaca gttggaaagt tatgaataag tttcggattc tattttcaaa
     4141 aggaacctta ctaaatcgtc tgttgcggaa atacgcggtt atcatgatcg gtgtaacgat
     4201 gactgctacc gttattttta gtatccatac ttgggaacaa aatcaaaaac aggcagaaaa
     4261 catgacaagt gatgccgttc aaagcacaag cagaatgctg aatgataaaa caaccttgag
     4321 taggatcatc aaaaaccagc ttgtagggaa ttccgaaaag atcgaaaatg tgacgaccta
     4381 cttaacaaaa cccatagatc agtatctgat gtatgtatac gaacagcaaa acagtaccga
     4441 tgaattagtt tctttcccaa atcaaatcaa agatctctac gcaaattatg aagaactaag
     4501 tgcgatatat atcgtgttga atcaactacc ggagtattat gaatcttcta gagaaaacaa
     4561 aggtggggaa ataaaaaagg ggacaccaaa attactagac gccttttata tcaagttgcc
     4621 tatcacacaa ggaggagctg agtcaggaag tatttttatt ggttttcaaa agaaagaact
     4681 agatatgatt ttagaaaatc taacgtcatt caatggactc tctttataca tgatttctgg
     4741 aacgacaaat cgactataca cattccatga taataaaata gctaaagaaa cgtttgaaag
     4801 acaagaaatg atcatcacag agagtctgaa gaaaaattca caattgccga ttgacgatct
     4861 ggaaaaaaac aattttgttc aacaccaaga actgtctgat gattatcgta tattagctgt
     4921 actagataaa aaaagtgtaa aaatcgaaac gggaaaaaat cttgccccgc tgatcattgg
     4981 tatcattatt ttagacaata tactgcttat ctttctttat aaaacattca aacgatattc
     5041 acagcaagta tctatcgtca tgcaggccat ggatcaaaca gcagcaggga atttagagac
     5101 gcggatcgac ataacaaaca cggaatatga actgaaggaa ctttcaatcg gtatcaacga
     5161 aatgttggat agcatcaatc agtttgtaga agacatctac aaattagaaa tcaaacagca
     5221 agacgcacat atgcgggcgc ttcaagcaca gatcaatcca cactttttat ataatacatt
     5281 agaatatatc cgaatgtatg caatcagcga aggaagtgaa gaattagcag atgttgtgta
     5341 tgcgttttcc gctttgcttc gaaacaatac gaatcaagag aaaacgatca ctttaaaaga
     5401 agaactggac ttttgcgaaa aatatgtcta tttgtatcaa atgcgttatc ctaatcgagt
     5461 agcctatcat tttatgatcg atcctgattt ggaaaagatc gaagtaccta aatttgtgat
     5521 ccagccatta gttgaaaatt attttaaaca cggtattgat tttactcgat tcgataatgc
     5581 gctaagtgtc aaagtcttgc aggaaggcaa acgggtaagg atcatcatca aagataatgg
     5641 aaaagggatg actgaaaaac ggctaaaaca gattgaagaa aaactgtctc atcctaaagt
     5701 ggaacttcac ggttctatcg gtctgcaaaa cgtgaatgaa cgtttacgtg ccagcttcgg
     5761 cagcagttat tatatgtcgc ttgagaataa tgagacaggt ggactgaccg tttctattac
     5821 attcaaggag ggatgagcgt gtatcaagtg ttattggtag atgatgaata catgattttg
     5881 aatgggttaa aaaagataat tgattggcaa agtttaggct ttcaaatagt agcaacagca
     5941 gagaatgcca aagaaggact agctgtactt gaacagcgac agattgattt agtagtaacg
     6001 gatgtgacaa tgccagaaat aaatggactg gaatttattg aagcggctca aaaagaacgt
     6061 cataattttg aatttatgat tttatcagga taccaagaat tcgattattt gaaaggaggt
     6121 atgcaattag gcgccgtgaa ttatttgatg aaacccgtga ataagttcga attggttgaa
     6181 agcctcaaga aaatcaaaac acggttagat cagcaaaatg aacaaaaaaa tcaacaagaa
     6241 atctatcaag aactgttatt cagtcaatgg ttgaatgaag aattggatga aataagtgaa
     6301 gaagaaataa taggacaaat aagtgagaag cagcatagga tagtattgat tcaactggca
     6361 agaactcatg atactcttat caatagatgg ttgcaagaac ggcaacaaat cttttactat
     6421 caacgaaatt acggagaccg tgtattgatc acgttgttgt tggaggcaga tgaagttgat
     6481 ggcttctgtt gttttgttca agaaaacttt cctgatcagg aatggctgat cagtatcggg
     6541 gaagagacag attctttgga caaaatccct gaaacattcc aacaagcgaa agacaatcta
     6601 cagttgcatc aattttatgg agataaagac gagcatgttt tttacgcagt acaagcagaa
     6661 acaaaagatc aaaccatcga tttttcaagt ttcagacgcg tgctgcaaaa taaacaattg
     6721 gaagtcgctc aaaaaatgat cactgatttt tttgaacagt ttcagttagc agccatgatg
     6781 ccggaagata tacgatattc cagtttttta ctgtttatgg agattcaacg ggaattgatt
     6841 gatctggaag atgaggaata tttacaagga atcgaaaaaa tccatcaggc aaaaacggtg
     6901 caagaacttc agcagctact tctgtcattc atcaaaaagc atcagagaca gaaaaagtac
     6961 agtgacaatg tggaaaaagt aatcgtaatc ctgcaccagc attatcaaga accacttaca
     7021 ctcaaagaag tgtcagaaaa cctgcattta aatgtcatgt atctaggaca gctgttcaaa
     7081 aaagaaacga aaaaaagttt ttcagcttat ttgaaccatt tacgtatgga aaaagcgaaa
     7141 caactgcttt tgcacagtaa tcaaaacatc aatgagatag caagtgagat tggctacaac
     7201 aacaccacgt atttctctaa attattcaag aaaatcgtcg gcagatcccc taaagagtac
     7261 cgagaaaaca gtggagaatc ataaaaaaaa aagccaatgt aatgaaataa cttataaaaa
     7321 attgtctaga aatctaattt tgggctgttc ttattacttg aggcaaggca gctttttcat
     7381 gacctctcga taaacggagt tcaaaagcca gtttctcggt attaagccaa atttatgcaa
     7441 aaatgtggaa agccattttc acaaaatttt tcttaatact cgaaactaaa cagctttttc
     7501 acgacctctc cactaaatgg tgttcaatca gctgaccctc ggtattagct caaaaaatgg
     7561 caaaatatga ggaactattt tgccatttgt ctctctaata ctcaggtcag aacgctgatt
     7621 tcacaacctc tcaataaacg gtgttcaaaa gcttgcatct gcggtaataa gaccaaccaa
     7681 acgaaaaata tgaggatcta tttttgcttg gctgttctta ttacttgatg caggacagct
     7741 ttttcacaac ctcttgttaa ttatagtttg ataaggaaaa aggcaaatct tttgcttgat
     7801 atttcttagg gtgagggatg atccctaatt ggtaagagat cgtgccgcct ttcagcaaat
     7861 cttgatgggt aaaatataat ttcgtatatt ctctgtccat ccgtttgatt ttatggataa
     7921 attgttgctg cggccgatta ggcgttgctt caatcgtcaa ttcctttcca tttgacaaaa
     7981 gcaaaacagc cttgtcaatt aatggcatac cgataacata ttcaccggtt ccaggcgtga
     8041 caggataaaa tcccagacta ttgaagatat accaagcggc catacttcca ttgtcctcat
     8101 ctccagggta acccattgga gaatcgtcga aggtctgagt catcaactgt ttaagcagtg
     8161 gctgtgccat ttcaggttgt cctatataac tgaacaagta aggatagtga aaacttggct
     8221 ggtttgaaat agctaattgt ccaaattcta tcgcggccat ctcactcatt tcgtggatct
     8281 caaaaccata tcctcctggg tcaaacgttg ggggctgatt gcacagctca atgagttttt
     8341 ctttatatct ctcactgcca ccatattttc cgatcagtcc tgaaaaatcg tgataaacag
     8401 caaagctgct ttgccaagca cttccttctg cataatcttg tccccaacga ttagctgaaa
     8461 atggttctct gaacttgcca tctttatctt ttgaacgcat aaatcctgac tcatagtcaa
     8521 agatattttg atagttcaat gattgctgct gatatttctt cgtcatttcc tcttcttcta
     8581 gtaccttggc aacttggcta atacaaaaat cgctataagc atagtctaat gtgtgattga
     8641 ctgattcgtg gtagtgatta ggaacatagc catatgtcaa gtaatcttct gttccttggc
     8701 gtccataatt tggatgtttg ctttgaaccg tagcaccttt ttccattgcc gttagaaatt
     8761 caggcatcaa gtctcgtcgg atatctttta cagcagcatc tgcaatgacc gcatcgatca
     8821 atgtcccagg cataagtcca cgttcatcag gagacagcca ttttggcaaa tagccacttt
     8881 ctctatagct gttcaaaaaa ccttccagca tttcttcgta tttttctaca gcaatcaaag
     8941 aataaagagg ataaaccgtt ttatacgtat cccaaaaccc attatttgta tagagaacac
     9001 caggttttat agatcgacta gtcgtatcat agtggatttt ttgcccacta ggatcaattt
     9061 cataaaatgt ttgtggaaat aaaaacaccc gatataaatt atgataaaac gtccgcaatt
     9121 gttgttgatc atggtgtgtg agctgtatac gatcaaaata ggactgccat tgttcttgac
     9181 tgtgagcaag gtaagtctct ttgctttggt tttgttctag ttccaggtta taagctgctt
     9241 gttccgagct gataaaagat gtcccaaatc ggatagtttg tttttttgtc tgttcaaaat
     9301 acaacgggat cgagtcgcta tttttgttgg aaacagctaa ctcatcaatt gttaaaggat
     9361 attcaaatct caatataaaa taaaaagaaa aatcaggatc ttctgaacca gcaaaatgtg
     9421 tgatttttcc ttccactgtg taaggatcta taacaaagag ctgatggcgt cccggaaagg
     9481 ataaaagtaa accgctgtca tttttgctgt aatcaataga taagattccg ccatacatgc
     9541 taggtatcaa tgtagaacag atgccataac gtaattgaca gatcgacaga tgagtcgggc
     9601 aaaatatact ttcttctggg cggtaagaac tttgagcatg gaaaagggtg ttctcctgta
     9661 gttttccatt gatcggcgta aacagcatat gactgaaatc acccatccaa ggacttggtt
     9721 gatgggtaag acggtagcct tgaaaaacgc ggtcatcagg atgaaaccac cagctgccgt
     9781 tttgatccgt agtttgcggt gcaaaataat tcatcccaaa aggaacgcca gtataaggct
     9841 ggcagttccc attagagaaa tttggctgat tcgctgttgc atgtctagta tctatttgac
     9901 cgatattcat tttatttctc ctttgttgat tgtcttttga tgaatgaaac tggaaattta
     9961 ctatcggatg gtcgatcttt tgtctctaac caagtgacca aagcatctcc cgcaagctta
    10021 cctaactgtt caaaatcttg tgcgatggtt gtcagtggcg ggtcaacaag agctgctgct
    10081 tggatatcat caaatccgat aattgaaaaa tcttcaggga tatgataacc atgctgtctc
    10141 aagagattca tggtctggat agcgacaaca tcgttttcac aaacaaaagc agttacttca
    10201 tacttagtga tataagaaag caattcatct aatgtcgcgc ttttgtcatc cagtgaagtg
    10261 tggaaagtca aaccagcttt gtccaatgct tcaaggtaac caaggtaacg ctgtcgaacg
    10321 gattgtggat gagtctgcgt accaaacaaa taagcgattt ttgaatgtcc ctgttcgatc
    10381 aaataagagg cagcaagcgc accgccttgg aaattatcgg aaagaacggt aggaaaaggt
    10441 aactcataga tttttttatc taatatgacg acagggaatt ctttcaatga aagctcaaaa
    10501 agtaagtcaa gatgttgttc tgtatcaaga gcataataaa tcaatccgtc aaattcattg
    10561 attagctctt ttgctgtttt attttgtaga aagtcaagcg tcgtcatcat gacatcaatc
    10621 tgtttatctt gcatcacagg gttcaatccc ttgctaaaat caccaacaga caaatcagcg
    10681 acatatggaa gaagaaacag aatccgtcgt gtttttgtag gagaagcaag aatctgttcc
    10741 ggagaagcgg atttaacgaa actgcctttc ccgcgaacgc gataaataag tcctaattgc
    10801 tctaattcag tcaatgcacg tttggaagtg attctgctta ctttataggt acgtgaaagt
    10861 tctttttcag ttggtagttg gctgtctaca ggtaattcac cagttaaaat agccgtttgc
    10921 aaatcattaa atattgtctg gtacaatggt tgagtcatat aaacacctct taatgatata
    10981 tcatattcaa ataataccat aatggatgaa aaagtaaata aaaagatatt ttttgaaagc
    11041 gtagacataa ttggctgtat gaagtataat ctttaaagat atatcaaata gaaagaaggt
    11101 tttattagtg gtttataaag atataccaaa atcggtgcag aaatttatgg aagatatcac
    11161 acaaaaatgt ggggatatcc atgcggactg ggcaaagaat tttacagcag catttgccaa
    11221 tactctgttg acaacagtca aaaagcaaaa tgacggaacc acctttttat tgacgggaga
    11281 cattccagcg atgtggttaa gagactctac tgcacaagtt cgtccttatt tagtgattgc
    11341 aaaagaagat gaggaattag ctgacatgat cagcgggtta gtcaaaaagc aattttttta
    11401 tatcaatatc gatccatatg cgaatgcctt caacgaagaa gctaacggtg ctggccacca
    11461 aacagatcat acaaaaatga acgattggat ctgggaaaga aaatatgaaa ttgattcttt
    11521 atgctaccca atacagttgg cctatcttct ttacaaaaat acaggaaaaa ctgatcaatt
    11581 caatcaatct tttattgaag gtatcaaaaa aatcttggac gtgttcaaaa cagagcaaga
    11641 tcatgaacac tctccttata catttgtacg agacacgaca agaaaagaag atacgctgat
    11701 caatgacggg cgaggtgcaa aaacagcttt tacaggaatg acatggtctg gtttccgtcc
    11761 tagcgatgat gcttgccgtt atggatatct ggtaccatcc aatatgtttg cggtggtcgt
    11821 gttaggttat gtggaagaaa tttttcaaac gatactcgat gataaagaaa ttgtacagca
    11881 aacacagaaa ttgaagaaag agatccaaga ggggattcag aatcacggac gaacggttaa
    11941 tcaaaaaggg caaacgattt atgcatatga agtagatgga atgggaaacg ccacagtcat
    12001 ggatgacagt aatgtgccca atctgatcgc cgcaccttac ttgggatatt gttctgaaca
    12061 agaggaagat tatctgcaaa caagaagaac attgttgagt aaagaaaatc catatttcta
    12121 tgaaggaaag tacgcaaaag gaattggatc ctcccatacg ccagaaaaat acgtttggcc
    12181 gattgcatta gctatggaag gaatgacaac gtcagacaaa tctgaaaaag agcggatatt
    12241 gaatttactt gtagcaactg acgcaggaac acaccttatg cacgaaggat tcgatgtgaa
    12301 caatcctgaa aattatacga gagaatggtt ttcttgggca aatatgatgt tttgcgaatt
    12361 agtcatggat tactttgata ttagagtaga aaagtaggag gtatcgtaaa tgaaaaagaa
    12421 agtatatatc atttctcata gccactggga tcgtgaatgg tacatggctt acgaacagca
    12481 tcacatgcga ctgattgaat tgatagacga tctactagag ctatttgaag ttgatccgtc
    12541 cttcaacagt tttcatttgg atgggcaaac gattatttta gatgactatc ttcaagtaag
    12601 gccagaaaaa agacaggcaa tccaacaagc aatcaatgaa ggaaaattga gaattggacc
    12661 attttatatc ttgcaagatg attttcttat cagctcggaa tcgaacgtga gaaatatgct
    12721 gattggcatg gaagaaagcc gtaaatgggg aacccctgtc atgttaggct attttcccga
    12781 tacatttgga aatatgggac agacgcctca attaatgaaa caagctggta tttcagcagc
    12841 agcgtttggc cgtggtgtaa aaccaattgg ctttgataat caagttttgg aagcagaaaa
    12901 ctattcttcc caatactcag agatgtggtg gaaaggccct gatcaaacag caatctttgg
    12961 tctgctattt gccaattggt atagcaacgg gaatgagatt ccagtagaaa aagaagcagc
    13021 tcttgccttt tggaagcaaa aattagcgga tgcagaacaa tatgcctcta cgaaccactt
    13081 attgatgatg aatggtgttg atcatcaacc ggtacaaaaa gatatatcaa aagcaatcca
    13141 tctcgcaaat gaactgtttc cagattacga gtttattcat tctaatttta ctgactatct
    13201 ggaagcagtt caaaaagatg tgccagaaga cttaggctca gtagaaggag aattgacaag
    13261 tcaagaaacg gatggctggt atactttagc caatacagca tctgctcggg tttacttaaa
    13321 acaatggaat accaaggtcc agcgacagct agaaaatatt acagaaccac ttgcgacgat
    13381 ggcttatgaa gttagtggaa actatcccca tgatcaatta gattatgctt ggaagacatt
    13441 aatgcaaaat catcctcacg acagtatttg cggttgttca gtcgattctg tccatcggga
    13501 aatgatacct cgttttgaaa aagcagatga agttggaaag tatcttgcac aagattcctt
    13561 agaacagctg accgcagcaa tcgatacgac cggattccca aaagacagct ttccatttgt
    13621 cattgtaaat actgcaggaa tggataaaac tggtgaagca gaaataacca tcgaattaga
    13681 aagaaaaaga ttcgctgaag gaatacctga acaactatat caagagttag aaaatttgcc
    13741 taaacgtaag tatcatgtag aaacaaaaag tggggccact ataccagcta ttttatctga
    13801 agaaactgtc caatttggtt atgatttgcc gaaagaccgt ttccgtgtgc catatatggc
    13861 aagaatgatc aaagtgactt taccattaga aaacatgcca gcattttctt gggaaacatt
    13921 cgcgcttgta gaaggggaag cgaaagcaga agaaaaggaa acaatgattc atcagagtgg
    13981 acgaatcatc gaaaacggcc ctctacattt gacgattgaa aaaaatggta ccattacaat
    14041 ggaagatcgc aaaaacaaac gaaagttgaa taatctgcat atttttgaag atatcggcga
    14101 tataggaaat gagtatatct ttaaacaacc tttctgtgat cagccaatcc tatccagtaa
    14161 caaagaaaac tctgaagtaa aagtattagt agatacaccg gaaatagcaa aaataagtat
    14221 tctacaggaa atggaaatcc ctgtttctgc agacgagcgt ttagaaaaag aacagcagat
    14281 ggttgtggaa tttcgttatc gaaaagcaga acgatccaaa gaaaaacgaa tccttcaaat
    14341 aaaaacaatc atgacaatca gaaaagatag taaaaaaatc gactttgaaa cgactctcga
    14401 caatcagatg aaagatcatc gcttacgtgt cttgtttcct acaaagttac atgtggaaca
    14461 tcacgaagca gatagtattt ttgaagtggt gaagcgccca aaccatgtgt ctaaaagttg
    14521 ggaaaacccc acgaatccgc aacatcagca agcatttgtc aatattcatg atgaagagta
    14581 cggtgtaaca gtcggaaact ttggtttgaa tgaatatgaa gtaacagaag acggacaaat
    14641 cgcagtgacg ctacttcgca gtgtgggaga actaggcgat tggggatatt ttccaacacc
    14701 agaagcacag tgtctaggtg agcatcggtt caattacagc attgaactac atggaccaga
    14761 agaaaaattc tctacttatt tgcatgcata cgcagcacag attccttttt ccactcaaca
    14821 aatcaaacat catgaaggaa cattgatttc aaaacaacaa tatttaacga taaaaagtga
    14881 aacgtttgca attacagctt tgaaacgcag caagtttagt gataaagtag tagtaagagg
    14941 tttcaatatg tctagtcatt tagaaaaatt ggaaataact aaagacaatg gaaaaactgt
    15001 catattgaat ctgcttgaag aaccaacgaa gcaagcggtt gtcccaataa tccaacctta
    15061 tgagattcgg acaatcggat tcgaggagga aaacgaatgt atggcggaat agaagcaggc
    15121 ggaacaaaat ttgtgtgtgc ggtttcagat ggtggagaaa ttttagaaaa attaagtatc
    15181 cctaccacaa caccagaaga aacattggat caggtatttg aattttttga tcgttatgaa
    15241 ttgaagggaa tcgggattgg ctcattcggc ccgattggta tcgacaaaac aaaagataat
    15301 tacggatacg ttttggcaac tcccaaaaaa gggtgggcac aattcgattt tcttggcaca
    15361 atcaaggaac gctacaatat tcctgctgct tggacaacgg atgtcaatgc tgctgcatat
    15421 ggggaattga agcaaggagc agcacaagga aaaaatagtt gtatttacct taccgtggga
    15481 acaggaatcg gtgcaggtgt cgtgatcaat gaagaaattt tttcagggat cgcacatcct
    15541 gaaatgggac atatttgggt gaaacgccat ccagatgacc attatgaagg tacttgtcct
    15601 tatcataaag attgtttaga aggattagca gcaggaccgt ctattgaggc acgcacaggg
    15661 atcaaagggc aagatctgcc gcaagatcat cctgtttggg ctattcaggc ttattatatt
    15721 gctcaagcac tgatcaacta tacgttgacg ttagcacctg aaaagattat tttaggcggt
    15781 ggggtaatga atcaggatca tctccttcaa aaaatccgtc agcaatttgt tgaattgatg
    15841 ggtggttata tggaaacacc tcaagtaagt gaatatatcg ttcgttgggg actgccgaat
    15901 gaaagtggga ttatcggtag tttgctgtta gcagaacacg aacatcaaaa gtaagcagac
    15961 aaattacaca aaaagaacag agtcttatca ttatctgctg ataagactct gttctttttt
    16021 atttctattc ccataatcca ggtaaagctt taccgactat tttttcttga gtaaatggat
    16081 ggatcatgtg cagttctaat gcatggagca tcagtcgttc agctgttcta ttttggtaga
    16141 gagggtctcc aacgatcgga tgtcccttat gagctaaatg aactctgatc tggtgtgtac
    16201 gtccggtttc caaaacacaa tagacttgtg tttgttcaga aagtactttg ttgacagtga
    16261 catgtgtcaa ggcgctctga ccatttttat catctacgac cctttttctc cgatcatggc
    16321 gatctcgtcc aattttattg gtgatggtca tatctttggc aatttctccc catacgatgg
    16381 cttgatatct ccgatagatc attttttgtt ccaacattct tccaagtatc ggcaagacaa
    16441 atggattttt tgcaaataaa acggcaccgc ttgtttcttt gtccaagcgg tggacaacgt
    16501 aaggtcgttg ctgtttagtg tcgagataag cagctaaatg attgagtaag gtactgtttt
    16561 ctgtcggttc atttggatga gtcttcattc ctgcaggctt atccactata atcaaatgct
    16621 catcttcata taaaacacgg atgtgcttcg catctcctaa ttgaacttct tgataattat
    16681 aatctgtttc ttcgaataac aatgtgatct ggtcattgcc ctttacttca tgatggaaca
    16741 aagcaggttg ttgattgatc caaacgtttt tacgtatacg caaaaagtgt cgaacctttc
    16801 taggaactaa ccattccttt tcaagcaagt cgcgaattgt tgtttcagga tggttcatag
    16861 gtaaagtgat cgtgattttc acaagtgtac tcctttcagt gcaatcgcag actaattgta
    16921 ccatttttaa aacatattta agaaaaaact tacatttttc tgttcattct tatgttactc
    16981 tacttgcact aacgtaaaaa aacatattga actacaactt aagcaggaag aaaacactct
    17041 ttcactatgg taaaataaga cgattgaatc aaatagacag gagaagaagc aaatggattt
    17101 tcaaagcatt ttagaaaaga taaaagctgc tttgatcagt ttttggcgtt ggatcagacc
    17161 atatctcgga caattccatc gatggcgaaa gcgcatctgg aaaaaatatc atgtgaacaa
    17221 attgattttg ctgattggtt tgatctgtgt cttagtgaca agtatctacc tctttatttt
    17281 agcaaaacaa gcaaatgtcg aaacgttaaa atcaggatta agccaatcga cagtcattta
    17341 tgataagaaa aacgaagaag ctggcacact gtacgcccaa aaaggaacat atgtagaatt
    17401 aaacgcgatt tctccctata tccaagatgc agtaatctcg acagaagata ggaattttta
    17461 cgagcatcac gggtttgata tcaaagggat tgcccgtgca gcagtccgaa tggtcttgtc
    17521 tggcggaaca tcagggggcg gaggaagtac gatcactcag cagcttgcca aaaatgcgta
    17581 tctgacatta gaccaaacgt ttgatcgaaa agcgaaggaa ttatttttgg caatcgagat
    17641 cgaaaagaag tacagcaaag aagaaatctt gaccatgtat cttaacaatg cctattttgg
    17701 caacggggta tggggtgtcc aagacgcttc cagaaagtat tttggagtag atgcaacgaa
    17761 tgttacgctg tctgaggctg ctactttggc aggaatgctg aaaggtcctg gtatttataa
    17821 tccgatcgat cacccaaaaa atgcgaatga tcggaaaaac acagtactaa gtgtaatgga
    17881 agaaaacgga aaaatcacaa aagaacaaga acaagcagaa agccagacag atatcagcgc
    17941 gtatcttaat gacacatacg ataattcaga ttctggttat cgttatcctt attattttga
    18001 tgcagtgatc gatgaagcag ttaacgagta tggtcttgat gaagaagatg tcatgaataa
    18061 aggctataaa atctatacag ctttagatca ggattatcaa aagcaattag aagctactta
    18121 tcaaaacagt gcattattcc cagcaaatgc ttcagatggt gccatggtcc aatctgcttc
    18181 agtagcttta gatccgaata caggtggtgt acaagcacta gtaggacgaa gaggagatca
    18241 tgtctttaga ggattcagct ttgctacaca gatgcgtcgt tcaccaggat caacgatcaa
    18301 accattaagc gtatatacac cagcactgga agctggatac aagccttcca gtatcttgaa
    18361 agacgaaccg caaagttatt acgatgcgca taattttgac gggacatacc aaggagaagt
    18421 accgatgtat caagctgttg cacaaagttt gaatttgcct actgtatggc ttttgcatga
    18481 gatcggttta caaaaaggat atgataaagc agaagaattc ggcctgcctt tagcaaaatc
    18541 tgataagtat tatggcttag cccttggtgg actggaaaaa ggagtatctc cattgatcat
    18601 ggcgggtgct tacagtgcat ttgccaatga tggtcagatg tatacgccgc atcttatcac
    18661 gaaaatcgtt gattcgactg gagcggtgat cgtcgataac acaaacaaaa aaccaaaaca
    18721 ggtcatatca aaggaaacag ccgatgaaat gaccagcatg cttcttggta cattttcaaa
    18781 tggaacagcg gctgctgcga atcctgcagg ttatacgata gcagggaaaa caggaaccac
    18841 agaaactaat ttcgatgcca caaaagcaaa tgaccaatgg atgatcggtt acacgccaga
    18901 cgtagtgatt tcgacatgga tgggttacga agaatccagc aagctgcact atttggaagg
    18961 gacaagtgga aatgttgtcg ggaaagtctt caaatcagcg gcagaaggca tccttccgta
    19021 tacttcaaaa acaggattta gtgtagcaga tgcctatgcc acaggaggac aagtggtagc
    19081 tgcagatcaa gttggaaaca caggaaattc taatggagag tctggaaatt ggcaagataa
    19141 cttgaatcaa tatggtgaac aagcaaaaga agggctgaaa aacttcggcg atatggtgaa
    19201 agaaggcgtt cagggaattg gtgaagcggc taaagacctc tggcggaaat atcaaggaga
    19261 gtagcccctt tgaaaacgaa taaccccatg ttacaataga gagtaactac taaaagaaaa
    19321 gagggcataa catgtcaaac atctacgata gcgcgaatca aatcgaacgt gagatccgtg
    19381 aattaccaga attcaaagcg ttgaaagaag cgtacgagaa agtacaagca aacgaagaag
    19441 cacacaaact gttcaaagac ttccaagcga tgcaaatcga attacaagaa aaacaaatga
    19501 gtggacaaga gttcagcgac gaagatgcaa tgaaagcaca agaaatggct gtgaaaatcc
    19561 aatcagaaga agtaatcaat gacttgatgc aaaaagagca aggcttcagt acaatcatca
    19621 atgacttgaa ccgtatcatc atgacaccag ttcgcgacct ttacagcgaa taaaactatt
    19681 ttatgaaaca acagagggtg tgaaagagac gttcggcctt gagcgatgac aaaattgctt
    19741 cttattgccg ataggctgtc tcttgaacac catttaccaa gaggtcgtga gcctaccgtt
    19801 taactctgag ttacaaggaa caatttcaaa aaacagcttc tcatgtttga tgactattgt
    19861 gacttgtgac cgaggagttg gcaggagaac accgtttatt cagaaatagg agctacgaca
    19921 aaagtcttgt catagctcct attttttgtc attcattttt caagcggtag aaatagcctt
    19981 tttgagctaa cagtttttcg taagtaccta tttcaacgat ttttccttca tcgatcacaa
    20041 cgattttatc tgcctctttg atcgttgaaa gccgatgagc cacaataaaa gtagtgggtg
    20101 tttctgtcag gtaattcagg gcttgctgga tttttttctc tgactggtta tctaaagctg
    20161 aggttgcttc atctagcaaa atgatttttg gctgacgtat caacgctcgt gcaatggaga
    20221 tacgttgctt ttggccacca gacagcttgt tgccatgttc gcctactaag gtatccagac
    20281 catcaggaag ctggtcaatc agtgaagaaa gttgcgcagc ttcgatgact tcatctaaac
    20341 gttcttttga tacgtttttc aagccataag tgatgttctc tttgatcgaa gcggagaaca
    20401 acaaagtggt ttgaggaaca acggctaaat aatttcgcac acttcgcata tccagttcat
    20461 ccaaaggctg atcatctaga aaaatttttc ctgaagtcgg ctggataaat ccaatcaata
    20521 agttaaggag tgtagatttt ccagagccag atggcccaac aaaagcaatt gtttctccag
    20581 ctttcacctc tagagaaaaa tgatcgataa tcggtttgag tgagtccgga taaagaaaag
    20641 aaacatcctc aaaacgaaaa tttccatcca aatgttggat ttttttctta ccgtgataag
    20701 tttctacatc gccagcaatc aacagttcgc caatagaagt gatagattcc atgccttttg
    20761 aaatcgtagg aatcagcgtg atcattccag aaacggaatt gacgatcgtc gtgaaatacg
    20821 tctgatacat cacgacatcg ccgggttgga tgcgcccttg taaagctaat atcccagtga
    20881 aaatcaaaca aatcagctga aatatctgaa aactagccca gctgactgat ccgaaaacag
    20941 attgcaggat atctaagtgg aatccttttt catagatttg agtaaaatgc ttatttaagc
    21001 gagttgtttc gtgtttttct aacccatgag ctcgagcaat cggtgctaat tcgatcactt
    21061 cgatcacttt tgcagaagtc tcttccattt cttgcctgaa gtctgagttg gactgtctga
    21121 tccttttacg gaaaaagaga gtaacgaatg cggcgagagg aacagtcaat aagaaaaaca
    21181 tgaaaacaac aggactttta taaagcgtga tacctaaagc tacgaataaa ttgacagcga
    21241 tatttaatcc gctgacaaat acttgagagg acaacgtttg gatagcttct acatcacgga
    21301 tgatctttga ctggattcgt ccagattgca attctttttg ataaggaata gatagttctt
    21361 ggattttctt gataagggtc gctcttagtc cagcttctac ttgacggatc gaagtgctat
    21421 ggtattttac atgaaaataa ttcgtcaata tattttgacc aatcagacaa gccatcaaaa
    21481 aagcgttgaa ataaatggcc tgcaactgag aagaatcttt cgatgaagct gcattgatta
    21541 cattggcaat agcgatcggc attaaccaag atggaagatg tttgataata aaaaagagtc
    21601 ctgacatggc aaaacgccaa taatttccct gataaagatg cagcaatgtc tgaaaagcat
    21661 ttgcatgata tttttgataa cttttctgta ttcgttcctc cataatcttc tccttaacaa
    21721 tcttggtaaa taaactgcaa gtgttctatt gcttcaggaa cataaatctc aggagtagct
    21781 tcaagagtgg cgtacattag aggacgctca tatttgatat aatgcagtac cgggcggaaa
    21841 tctaaacagc ccttgccaac aggtaccatg cggatctttc cattttccat gatatagtct
    21901 ttgagatgaa gcgctgtaat ataaccatgt aaattgtcca aggcacctcg gatcactttt
    21961 tcctgatcag catgattttc tttatgcatc aaattcgcac aatctaaaat gatttttaaa
    22021 tttggtgaag ctacttcatc gattaaacgt ttagctaact gatagttata gattggatga
    22081 ttgatccctg cttcaacagc aacagttacg ccaaaatttt ctgcatgagc gactaattca
    22141 atgatagaat ttctggcgat ctgataagct tcttcagtaa aattttcttt tgtgtaaccg
    22201 acagagacac taccggtttc cgtagcaatc atcttggctt gataggcaga gcacagagca
    22261 atttgctgtt tgaaagttgc caatgcttgt gcacgaactt cttgattttt gctggatagg
    22321 ttgatatagc atcctaaaat ggaaagttcg atattgtttt tttggaagta ttcaccaaaa
    22381 taggcagcac tcccaagtgt catgtcagtt aatttaagtt gttcaggaaa tgctttttgt
    22441 ggtgcaaatt ggatatggga caagtgaagc tgtgatactt tttgaacgat ttcttcacga
    22501 tttcgaacat ctaaatcgtg ggcacggatg cctaaattta atggaaacat ctcaaactcc
    22561 tttttgtaaa taattagcaa gaggcagacg ggattctttt atcaattcaa caattattcg
    22621 ggcaacttta tcagcgcctt ctggactaaa gtgggtattg tcttttagtc cttctgggta
    22681 atttttacaa gtatcaggta taagatgcag gtagaatttt tttgtctctt cttttgagaa
    22741 ggtctggaac aattcttgtg atcttgagaa cacatctaaa agaggaatat ggtgtttctc
    22801 agcaaatgca cgcatcgctt ttggatagtc tccaagtata tctggattca atgttccgtt
    22861 ttctaagtaa tctcgtcgcg tcacagatgt cagtaagacc atttggacgt tcgctttttt
    22921 tgcgcattca aaaaatatct gcagattttt taaatagcta ccgaagggaa gcgtacctct
    22981 ttcttcttgg attttctggt cgttatgtcc aaattgtaca aataagaaat cattcggctg
    23041 aaactgtgaa gctagacggg ctaaccttcc ttcctggata aatgatttgg tgctgcgtcc
    23101 attctttgct tggttattga tctggatctc gggtgaaaaa taagcagaga ttttttctcc
    23161 ccaacctgtc tcgggacgct tgttttcatc tttttctgcc gcagtagaat caccggcaat
    23221 atggatagta ggcatgttat ctcttctttc ataaaggttt ataggcaatg aagcagaaca
    23281 gcaagagaag aagacggagc aaatttggtc cgtcttcttc tctaaaacgt ggataaacgg
    23341 attccgttat ttcactatcc tgtttttgat tcgttactgt tactattaat caattaggca
    23401 aaaaagccaa agtaattttt agcatttgta taactaatcg cttgaactag acttcctaaa
    23461 agttgttcat cttcaggtgc ttctcctcgc atgaccattt cgcctaaaaa ttcacaaaga
    23521 acacgacgga aatattcatg tctagtgtag gacaggaaac ttcttgaatc tgtcagcatg
    23581 ccgacaaaat tccccaaagc actaccatca gcaaattgtg tcaattgatc gcgcatacca
    23641 gctcgagtat cgttgtacca ccatccagaa cctaactgca gttttccatt ctgttctttt
    23701 tggaaacagc ccatcaaagc taaaatcgca ggatagtcat tcggattcaa tgaataaagg
    23761 atcgtctttg gcaactgatc cgtttcttct gcacggttca ataatttttg cagaggaata
    23821 gtcaaagaaa gatcattcat tccatcatag ccggtatcgg gccctaattt ttccaacatt
    23881 tgtgtattac agttacgata agcatggatg tgcagctgca tcgtccaatt atgagaatga
    23941 tagaagtggg tcaaatgtat cagtgtttct gtacgataag catcgatttc atctgaagat
    24001 aatagtttgt tagctcgtgc tttttgaaaa atctcttcta atactgtttc atccgcttct
    24061 ttatagcgta agatgtctaa agcgtggtcg gaaacacgac atccatgttg atggaagtat
    24121 tctatccgct gccctaagac ttctatcagt tcttgatagg tggtgatcgt ttggtcagct
    24181 gcttcttcca gtagtgtgac ccatgcatta aatccttctt tttcaatatt gagtgcttta
    24241 tctggacgga atgaaggtag aaccttcaaa tctggttctt catgagcaag caactgatga
    24301 tagtgcaagt cgtctgcggg atcatctgtt gtacaaatca cttctacatt cgattttttt
    24361 gcgatttccc gacggcgaaa atcaggagct gccaaaattt catttgcttg tgtccaaatt
    24421 ttctctgcat tttcttcatt tatcaacaag tcaataccga agaaacgctt caattcaaga
    24481 tgtgaccaac tgtaaagcgg atttccgata atttttggta cggtttgaca ccaagctaaa
    24541 aatttttcaa aatcagaagc atttcctgtg attttttctt caggcacgcc gcaagctcgc
    24601 atcaagcgcc atttgtaatg atcgcctcct aaccaagctt ctgtcagatt gctgaaattg
    24661 ttgttttcgt aaatttcttt tggatctaag tgacagtgat aatcaataat gggcatgttt
    24721 tttgcgtagg aatggtaaag ctttttcgcc caatcgtctt ttaataaaaa atcatcagat
    24781 aaaaacataa atcttccttc tttcttttta tactgtttta tgaaaatcaa agtgttactc
    24841 cgtttttaaa aatggcaatt tctcgaacgt cattttgttc atttttcgtt tcttctccac
    24901 tagctacagc gatgattttt tgaacaaacg attctaagat tacttccatt ggttgttcta
    24961 ataagacgcc tgcattaaaa tccatccaat gacctttgcg ttcaaaaaga gttgtattcg
    25021 tagatacctt gaccgttggg acatatgagc caaacggagt ccctcttcct gtggtaaaca
    25081 agaccaactg gcaatcggat gaagcaagag cagaggcagc tacaagatca ttcccgggag
    25141 cttgcaataa actaagccct ggttttctga ttttttcgcc atattgtagc acatcaacga
    25201 caggagagct tcctgatttt tgcgtgcagc ctaaagactt atcttctaat gtagtgatcc
    25261 cacctgcttt attaccagga gatgggtttt cgtaaactgg ttccccgtaa gacaagaaat
    25321 actgtttgaa gtcattgatc aagtcaacga ttgagtcaaa aacttcttca ttttccgctc
    25381 gtgccatcaa tacttgttct gcgccaaaca tttctggcac ttctgttaag atcgtgctgc
    25441 ctccttgagc aatcaagtag tcagaaaatg caccaagtaa aggattagca gttattcccg
    25501 atagaccatc agaaccgccg catttcaaac cgacattcaa cttagagagc gggactgata
    25561 tgcgctggtc attttccgcc gctgttaaaa gctcttctaa tagagccaca ccttgttcga
    25621 tctcatcgta aacatcttga gccactagaa atttcactcg ttcttcatca tattcaccta
    25681 aaattttttt gaattcagga actgtattgt tctcacaacc taatccaaat actaaaactc
    25741 ctccggcatt gggatgattt actgcgtccg ctaaaatttc tcgagtattt tgatgatctt
    25801 ttccaagctg cgaacagcca taaggatgct ttaaaatcgt aatatggtca aactgtccca
    25861 gatctggatg tttttccttg aactgtttga cgatcagttc agcaatgcca ttcacacacc
    25921 caacggtagg tactaaaaac aaatcattac gaatccctac ttttccattt tttcgcaagt
    25981 aaccttgaaa tgttcggttc tcaaaaaggt aatgacttgg atggacatct ttttcatatc
    26041 gataatccaa ttcaccagaa agattggttt ttacattatg agtgtgcagc cattgaccgg
    26101 cagttacgtc tgtcgtcaca tgaccgattg gatagccata tttgatcacg ttgtctccag
    26161 ctttcaaatc acccaatgca atcttatgac cttgcgggat atcttcaaga gtggtgatcg
    26221 tttgctgatc aatcgtcagt tgtgtttgtt ttggaatagg ggttaatgcg acgaccacac
    26281 tgtctcgtcg atttaatttc attgttggtt gcagggtttc aagcatggac catcgttcct
    26341 ttcatttctt gtaatactga ccggacacct ttttgatcga tctttgcagc tatttcttga
    26401 actgtatctg tcacattcgg tatttgagac aaatcttctc cccaaagcgc agcgttttgc
    26461 aataatcgag gaatagctgt tccattatca gaccaagcat cagtaaattc gcttatcgtc
    26521 ttttcatcat catgtggctt tacttgatcg cctcgataca taagaagtag cgcagctagt
    26581 gaaacagtga ttaacggtgg tacctgattt tgttcttcga tatacttctt gaacactgga
    26641 agcagacggg tgctgaactt tgaaacacta tttaatgaaa tcgcttctaa tttgtgatgg
    26701 gcaaaaggat tcaagaaacg ttctttgatt tggtcagcat aaatggctaa ttcatcttta
    26761 ggtaaagaaa gcataggaat caattctaga ttgaataact gatcaacata tgcggagaat
    26821 aaaggatctt tcattacttc ttctacagtt tccaattccg ccaacaaagc aagagggacc
    26881 atcgctgtat gcggtccatt aagcaaatgc accttacgtt cacgataagg agtcatatca
    26941 tctgtgatga tgacattcaa gccagcctgt ttgaatggta gttgattctc caattcttta
    27001 ggcccttcga tcacccaaag catgaatggt tctgctttga ccatcaattg atcttcatag
    27061 ttatgccgtt gattgaaaac acctgcatct tcgcgaggat aacctgggac gatgcgatca
    27121 actaaactac aacagaaaac attttctttt tctaaccact caacaaaagc aggttctaaa
    27181 tcccattttt ctgcgtagcg taaaatgatt tcttttagac gttcgccgtt tctgtcaatc
    27241 agttcgcaag gaatgatggt aaagccagct ttgtttaatt tgaaacgtcg gtataaaaag
    27301 gctgtcagtt tgcccgggaa gctctgtggg ggagtatctg ttaaattatc agtctccacg
    27361 aattggattc ctgcctctgt tgtattggag acgatatagg ccaagtcgtc attttctgct
    27421 agagatagat aagcatccca ttcatcataa ggattgatca tttgttctac tacggtgata
    27481 atctcggaag tatcaacgat ttcgccattg ctgagtcctt gtaaaatgac cgtatataaa
    27541 ttatcttgct ggttcatcat atttccaaga ccttgttcta aaggttgaat gattgcaaca
    27601 ttacctttaa acaattgttg gttattcagc tgctgaatct gccaatcaat aaatccacgc
    27661 ataaaattgc cttcaccaaa ctgaagaact tttacaggtg cttcgttttt tttcactaat
    27721 tcttttgtca gttttttcat ataaacttcc tttctgtgtt atgcacacgt taacattttt
    27781 atataaaatt acttgtagtt tacaagtaat tgatcgactc acgaaggatc aacaaggtgg
    27841 agagctcaat tttagattgt tcgatcgtcc tgccttcgat caaatctagt aaaagctttg
    27901 ttccttcttg acacatttct ttcaaaggct tacgtacggt ggtaagcggc ggattgagat
    27961 aggagacgac tggagaatca tcgaacccga tcagagaaat gttctgggga acacggaggc
    28021 cagctaaatg tacagctctc aatgcaccaa tcgctatgtc gtcgttgccg caaaaaacca
    28081 gagtaggcag gtcagaaagt gataataggt attccatgtt ttggaggccg ctttctaagc
    28141 tgtaatctcc tgttaaaaaa taggctggat tgacagggat gctcgctttt tttagacttt
    28201 tcaatacccc tttttcccgt tcttttgtcg aacgaaaatt tttctttccg ccaatataag
    28261 cgatcttcgt atgtccgcat ccaataccgt aatctactgc ctgcataaca ccggccgcat
    28321 catcggcagt gacgttgata accgctggat cattgatgtg acgattgaca acaacgaaag
    28381 gaaattcttt ttctttgaga tagtcgatga aaggctgatc atcatcactt tgactcataa
    28441 cgatcagtcc atcgtagcgt tgcaggttga tttctgttaa atcaacaaca tcgatacttt
    28501 caacagataa actgtaattt gtgtctaata ctgaatggat gcctgcaatg gaatctgcta
    28561 ggaaatttga tgaagttcct ttatgcaaac ttgagaaaaa tagtccgatt agatattttt
    28621 tttgattcac taaacctttt gcactatagt ttggcgaata attcatttcc ttagctagtt
    28681 gtcgtatttt ttgttttgtt tcatttttta ttaaactgct gttattcaaa gcacgagaaa
    28741 ctgtcgtatg agaaacgcca gctgctttag cgatatcttt gatcgttacc attccatcat
    28801 tttccttact ttttcacttt ttgttaacgc attcatcata ttacgataac tacgataagt
    28861 caatagatct ttgatatgaa aagattgcac acgttaaaaa ttacttggat ttttattttt
    28921 aattatgcta ttcttgaaag cggggaatgc tcatttaatt agatgagtat caattttaca
    28981 gaaaaatgaa agccttttat tttaaaagga ggagaggaag aaaatgaaga aaagtacagt
    29041 attttataaa atatttttac cgatgttttt attaggagcc gtacttgtca taggatttag
    29101 tttattcatt taccagaata cctatgaatc gatcgaatcg agttatttaa cagacaaaaa
    29161 gaatttgctc aggcaagtta aaactaacgt tgaatggaaa attagaacga ttgaatattc
    29221 gttttctacg tatggttcga ccaaaaattt ctcggacata ttcaagcatc cgctgacata
    29281 tacagattac tccacttatt ctgaaatcag gaaagaacta aattttatag aaacgattgt
    29341 tatggatgat aataattatg atttagtcag tttggctggt aaatggggag taatcaatgg
    29401 atcgttgagt caactgaccg aagaggaagt tgaaaagtat caacagaagt acataaataa
    29461 caaaaataat ttattttggc aaaagaaaaa caaaggaatc gaaatggtga tttctttgcc
    29521 aatgtttgaa aaagaaaaat atgcattggg aattgctacg atagagaaac atacaataga
    29581 acaagttgtt ggtgaaagtg gagaagacat actttctatt gaaaacgaag ttggcaacct
    29641 ttttactact cagacagtaa aaaataaaga gcaaccaact gtattaacgg acgtggatct
    29701 agattacaat cagctgaaag tgataagaga aaaagataaa acgtatatag tactgaaatc
    29761 agattataat aattggattt acagattaga agttgactct agtgcaattg gcgcaatgat
    29821 ccgcaatctg agaatcgggc tagttgcagt gtctttaaca ctggtaggac tcattggtct
    29881 tctttcctac ttattttcgg acagatttgc ccgtccgatt tctcaaatac aggaacgtct
    29941 gaatcttaaa tcaaaaggat tcagtgggaa agaactagat ttggtcgctc aatcagtcag
    30001 tcggatcata ggagaaaaag aagcgttaag tgcacactta gttacccaaa agccacagct
    30061 ggaaacgcta tttgtactta gtttatttag aaatcgagta gaaaggcgag aactagatca
    30121 gcggttgcag caatttggtt atagttctca aaatgactgc tattatacag ctttggttca
    30181 aatcgatctg ttagaagata gtcacggtgg ggagcgtgat ttgctacttt tagcgatcaa
    30241 caacatgatc acggaaattg tgccaaagga aaatcgaatg ctgccgatcg ttttgaatga
    30301 agaaatgcaa gccactattt atcgaatgga taaagatgat gcagaagcaa gcaaaaaagt
    30361 catggagtat tgccgactca ttcaaaaacg tgtaaaagaa tacttgaatt taactatcag
    30421 tataggcatc agtaaccggt tcgaaacgct gaatgaaagc aagcaatcgg ttgatcgggc
    30481 aaaagaagcc ctgtattatc gtgtaaatac tggtccaagt tcaattattt tttatcagga
    30541 aatcgttcct gctgctcatg aaaaaacact tattagatat ccgatagaag aacaaaatcg
    30601 attgtttgaa gccattcgtt ctggaaatca ggaagtgaag aatttagtac atgaattgat
    30661 tgatgcattg tttgcacaaa ataaaaatcc tttgagtcgg gaagtagttt cggtccgctt
    30721 gatcaatgaa ctcgttcaat taggacagtt attgggagta gattcaaaaa actttgataa
    30781 catgaaacaa atttatgtaa aagcactgaa caattatcat cccaaagaac tagaaaaact
    30841 tgttttagaa caattagttc tgccaatcac agaatgctct caagcaatca ctgatcatga
    30901 atttaaaaat ttgtcggaga atattatgca tatcgtctac acagaatatg atcgtgatct
    30961 gtctttagat gtgatcgctg ataggttgca ttataaccct aactatttaa gtaatgtatt
    31021 taaaaaggaa acaggagaaa aatttggtga ttttgttcaa aatcatcgtt tagaggtagc
    31081 aaaaaaatgg ttgaaagaga ctaacctctc cgtaaaagag attgcagaaa gattgcaata
    31141 tcgaaatccg caaaatttta ttcgtttctt taaaaagaag gaatcgatta cgccgggaga
    31201 atatcgaaaa aaatatttgt gaatcaagag gctgtttttc ttgtggactg attcaagcaa
    31261 taaaagaagt tagaataacc ctagtatttg tatttatgga tagttaaatt tcaaggctaa
    31321 gcacgcaaaa agaactgtga caaaagtctt gtcacagttc ttttttacga ataaatggcg
    31381 ttcaagagac aggctatcga caataaggca taattttgtg aaatatggga agctattttt
    31441 caaaatcgct tcttattgct cctgtgggtt actcgtcgca atgtttgtga gagtttttat
    31501 catcgctcaa ggctgaacgt ctctttcaca acctctgata cacggtgttc caggagtaga
    31561 ttcttcgaaa aataagccga aatttcacaa aaatttaaga agcaattttc gtaaatgcct
    31621 tcttatttct tgaagctgac cacttctgac acaactacca ttttgcgttc agtacataat
    31681 caccctttga cagaacctac gagtgctcct ttgacaaaat atttctgaac aaaaggataa
    31741 acaatcagca taggcactgt tgcaataacg ataacagcca ttttaactgt ttgtgctggt
    31801 ggaatcacat caacggcact actttctgct tgcatcccac ttgatacaat gacgatttgg
    31861 cgtaaaagaa tctgtatcgg ccatttagat gaatcgttga tatacaaaat agcagtcata
    31921 taagtgttcc agtaagtaac agcataaaaa agcgagatag tagcaataga cggtaacgct
    31981 aatggcagca taattttgaa aaatacacca aaatctgtgc aaccatccat ttttgctgat
    32041 tcttccaaac tatccggcaa ggcttggaaa aagtttctca taatgatcat attcattgca
    32101 ttaacagcca caggcaggat catggcccag taagaattga ccagccctag agaacgaaca
    32161 actaagaacg ttggaatcat ccccccgctg aacaacatag aaaatacaac aagaaagttg
    32221 acgaaacctc gaccaaacaa atactttctg gaaagaccgt aggccatcag tgaagttgca
    32281 cacatactga cgatcgttcc gacacctgta acaccaatcg aaacagcaag tgctctaaaa
    32341 atggtcggcg tagaaagaat gtagcgatac gcgtctaaag aaaaggtagt aggaaaaagg
    32401 ataaactttt tcgcgacgac ttcttgtgta gaagcaaaag atgtagcgat gatatttaaa
    32461 aaaggaacga tacagatcaa agctagcaaa attagaaacg tattgttgaa aagtgtaaaa
    32521 cttctttctc ctatagagac tttctttttc atatagatag gctcctcctc aaaataaatt
    32581 aaatgtttct gtttctatat gttcaattta tcatcggtga aagattacgt atataatcat
    32641 ttcctatgct aagtgtcaga tagaaaaaaa tagaaggttt gctatgcgta gtttatttgt
    32701 gaaaaaatac actttatcgt tttgtatagc acaaaaatca ttgctatgta agcgttttta
    32761 cttacggtaa aaatgattat atacgtaatt cagatagtta tctatcatga agtcataagc
    32821 agcaaacatt cacaaaacta aggaggggct tatggaaagt gtaaagaaac aagaaccatt
    32881 tcaactgacg ctatcgaaaa aagaagaaaa gctcagaaga cgaaaaaaaa taattgcagg
    32941 aatcaaaacg aataaatttt tatatgtgat gattctgccg ggagtccttt atttcttgat
    33001 ttttaggtat cttcctatgg ggggactcgt tattgctttt caagattatc aaccattttt
    33061 aggaataatg ggaagtcctt gggtcggttt caaacatttc attcgtttgt ttacagaacc
    33121 gacattcttt acattattaa gaaatacgtt gatcttgttc ggattaaata tcttcgtgtt
    33181 ttttccgatg ccaatcattt tgtctttact attaaatgaa gtaacgaaca aacgattgaa
    33241 aaacgggatt caaacgatca tttacatccc tcacttcatg tcttgggtaa tcattgtttc
    33301 gattacttat gtatttttga atgttgacgg aggggtattg aatgaattcc ttgcaacaat
    33361 gggatttgaa aaaatcagtt ttctaacatc ccctgaatgg acacgcacag tatatatcgg
    33421 acaagtgatc tggaaagagt tgggctggtc tacgatcatc tacctttcag cgataactgc
    33481 agtcgataca cagttgtatg aagctgcaga aatggatgga gcaggacgct tacgcaaaac
    33541 atggcatgtt acattgccgg caattcgtcc agtaatcatt accttgctga ttctaaaaat
    33601 cggtcagaca ttagacttag ggttcgagca tatgtacttg ttattgaatt cattgaatcg
    33661 aagcgttgca gaaattttcg acacctatat ttatacagct ggattgaaga atggacagtt
    33721 aagtttcagt acagcaatgg gattgtttaa aggggtcgta ggattgttta tggtaatttt
    33781 agctaataag attgccaaaa agctaggaga agacggcgtt tactaagata gaaagagagg
    33841 gaaagatatg ctgcagtcat tagataaaat aaacggaagg ctaaatcgta ttttacagtt
    33901 ggtttatttg aattttctgt ggggcggttt agtcatcgca ggtttgggag tttttacttt
    33961 cggtcctgca tcgtatgcga tggttgcagt gattagaaaa tggctgcgct caaaagaaac
    34021 gtttcctttg acgaaaacgt acttcacata ttttaaagaa aactatcgtg aaaccttgct
    34081 gatgagctgg atatatggcg ccatcgggat tgttttactt gtggatcttt ctttcataca
    34141 caattggtat cttaggatcg ctttgtatat tgctgcgttc atatatgtgc tctcagcact
    34201 ctatattttc ccaatcatgg ctcattatga ttggaaagga tattttttca aaatgaaaat
    34261 ggcattttta ttcggattct cacatcttca ttactcatta gtgttgatat taagtatggt
    34321 agggatttat ctgatgatca tccaaacaat gccaggtgtt ttaaccttta tcggtggcag
    34381 tctcttcttt tccgcagtca cgtggacagc aaattctata ttttgccatt tggaatggaa
    34441 gaactcatat acagaaaaaa cagaagaaaa caaaaatatt attaaaacag tgaaggagaa
    34501 aatttatgaa aaaagcaacg ctcgtaaaat taggtcttag ttttgcagga attctagcac
    34561 ttgccgcatg cggcagcagt tcaagtaaca gttcttcaga gacagaagat ggaaaagcta
    34621 aaatcacttg gttaaatatt ttgcatcatg cttcaccgcc tacagacacg attgtgaact
    34681 tgatcgaaga aaaaaccgat acggaactga cattcaactg gattcctgat gcttcaaaag
    34741 aagaaagatt aacaactgca ttggcttctg atgaactagg agatatcgtt acacttcaaa
    34801 ttgcgatgtt gaaaaactct tctgtccgca atgcgttaaa atcaggcgta ttctgggatg
    34861 tatcagaata tttaaaagac tataaaaatt taagtcaaat ttctgaagat agaatcgaag
    34921 ctgcgagtat cgacggtgcc ttgtacggcg taccgataca aaaagactac gctcgtgctg
    34981 gcttagtcat tcgaaaagac tggttggata atctaggact agatgttcca actaccatgg
    35041 atgagcttta tgaagtagct aaacaattta cagaaaacga tcctgatgga aatggtgtgg
    35101 acgacacagt tggttttggc gatcgtgcgc ctagtgatat tcgttatacc agctttaaac
    35161 ttttcacttc ttattttgga gcaccaaatg gctgggctgt agacgataat ggcaaattca
    35221 ctccagaatt tgatacagaa gagtatatga aagctttgaa tttttcaaga gatctatttg
    35281 agaatggcta tttggcacaa gactttgcag ttacccaaaa gactgatcag caagaacaat
    35341 ttgcacaagg aagaactgga atctacactg gaatggtgga tatcaagaat ctgaagacga
    35401 tgggtcaagg tattcaagat gatatggaat tagtgcctgt aaacaagatc tcagatggtg
    35461 aaggcggcgg ttatcatgtt tggtcagaag gaaatggtat tggcggcttg ctagcatttc
    35521 ccaaatctca agtaaaagac gaagcacaat tgaagaaatt acttcaattt gttgatgatc
    35581 ttttagaaga agatgtgtat aaattgatga ctggcggtat cgaaggaacg cattatgaat
    35641 tgaatgatga tggttctatc gaatttatcg atatggatct ttggcaacaa gaggtgcaac
    35701 cgttatcaag ctctcgtcct agtgaagtga cgttctcttt caaagataca gaccctgaaa
    35761 aagaattctc taatcaattg atcaaagaaa atgaagaatt tgctgtaatg gatccttctg
    35821 tgccattgga ttcaccgacg aacaatgaag taggatcaga aattgaaaaa atcgtcattg
    35881 atgctacggt gcaatacatc atgggacagc ttgacgaaga tggattcaaa aaagctgtag
    35941 aagactggaa agcacaagga ggcgatcgaa tcacacaaga gtacgaggaa gcatacaaag
    36001 cagcggcaca gtaatataga cgattgggac aaatacttct tggtatttgt cccaaatcct
    36061 attttaattt ttaaaggaga gcaaataatg gaaaactggt caaagaaatt agcaaaaagt
    36121 atcatggaaa gaacacctcg cttatatgaa gaaaagtggt ataaaggaaa atggtcttat
    36181 gactatggcg tcgtacttaa agggttccaa ttactttggg aacgaaccca agaaaaaatt
    36241 tattttgatt tcataaaaga caatatcgac tatttcgtgc aagaagatgg aacgatccgt
    36301 ggctacagcg ttgaagaata caatatcgac catgtaaata caggaaagct tttcttttta
    36361 ttgtacaaag aaacaggcga agaaaaatat aaaaaagcag cagaactcct ttcccagcag
    36421 ttggcaaacc atccacgcac atccgaagga gctttttggc ataaagaaat ttatccttat
    36481 caaatttggt tggatggctt atatatgggt tctccttttt atgctgaatt tatccagaat
    36541 tttagtgaag aaaaagatta ccaagatgtt ttgagacagt ttgaaatcag ttataaacat
    36601 ctattcgatc caaaaacaaa tttgctgatt catgcatggg atgaaaagaa ggtccaacca
    36661 tgggctgatc caaaaacagg tctatcccat catttttgga gccgatcgat tggctggtat
    36721 ttgatggctg cagcagatac ctatgaatta cttcatgaag aaacagattg ttctttgctg
    36781 aaagaaattc tagaaaagac actggctgca ttacttccat atcaagaaga aaacagcggg
    36841 acatggtatc aagtaacaac agaaacaaaa cggaaaggaa attatctgga agcttccggc
    36901 agtagtatgt ttgtttacgc aatggctaaa ggaatacgtt tgggactgct gaacaaagaa
    36961 gaatggcttc cacaagtgaa gaaatcacat caaggattac ttgatgaatt tgttctggaa
    37021 acaaaagaag gctggctgaa cttgaataaa aactgtcaag tagcagggtt aggcggagca
    37081 gatcagcgag atggttcata tgcttactac atcagtgaac caattatttg taatgatcaa
    37141 aaaggcgttg gtgcattctt gcaagcattg attgaagtgg aggcattata aatatgtatg
    37201 atattttgac atttggcgca tcaattgatg aattgaatac tgaagcaata caacaagcca
    37261 ttgatgctgc agcaagtgac ggaggcggga ccgtagttgt tccagcaggt gaatttctaa
    37321 caggagcttt atttttaaaa agcaatgtcg aacttcattt aagcgcagga gcggttctga
    37381 aattctctga cgatccaaaa gattatcctg ttgttcattc acgttgggaa ggagtccatc
    37441 gaaaagttta tgcttcctgt atctatgctc aaaatgtaga gaacatctct gtcactggtt
    37501 tcggtacatt agatggaaat ggaaaaaaat ggtggcatac ttttagaaat gaaccagata
    37561 atctcgctta tcctcgtcct aaattgatga gtttccataa ctgtcatcga attactgtga
    37621 aagatattaa attgatccag tcaccaagtt ggacgatcaa cccgattctt tgcagtaatg
    37681 caacgtttga taacttgacg atcttgaacc cagcggattc accaaataca gacggaattg
    37741 atccagaatc atgcaaaaat gtgcgtatta gtaattgcca tattgacgtg ggagatgact
    37801 gtattgcaat caaggcaggg actgaagata cgtacgaaag gatcgcttgc gaaaatatca
    37861 cgatcacaaa ttgcacgatg gtccatggac atggcggtgt cgtattagga agtgaaatga
    37921 gtggtagtat tcgtaatatt accatttcaa attgtatctt ccaagaaact gatcgaggaa
    37981 tacgattgaa atctagacga ggtcgaggcg ggatcgttga agatattcga gtatccaata
    38041 tagtcatgga taatgtgatg tgtccattta tactgaatct ttattatttc tgtggtccgc
    38101 gagggaaaga accgtatgta tgggaaaaaa aggcttatcc gatagatgaa cggacaccag
    38161 cattcagaag gatccatttt tcgaatatca cggcaaggaa tgtccatgct tctgcaggtt
    38221 ttatttatgg attagcggaa caattcatac aagaaatcac atttgacaat atcgatgtct
    38281 cgatggccca agatgcagtt cccggtgttc ctgctatgat gacaggaata gaggaaatga
    38341 gcagacaagg attttatatt ggctttgcgg gtaaggtttc ctttaaccag atgtcgattg
    38401 aaaatcacga gggaccagca tttctattag aaaatactaa agaaatcagt attgagaaat
    38461 tccaatcaaa tcaatcagaa aatatggatg aactggttgt tcgtaaaaac tttgcttatg
    38521 cagttgaatg attttttagg tgctccatct agcagacata cccctagtgg aaaacgatta
    38581 taccgagaaa aacaaaacgg ttttatgctt gaatactatc aattgtactt aaatgaattg
    38641 gaaacagtac cagccctcta tgcttatcca gaaaaagaag gaccgtttcc gccggtcctt
    38701 tacctgcatt ctcacggcgg agatttttca gtcgggaaaa gtgaattgat ccatggtgct
    38761 ccttacttag cttctccatc ttttgcagaa gtactgacag gaatgggata tgctgtttgg
    38821 tcgattgatg cttggggatt tgaagaacgc ggaggtattt ctgaaagcga gttgttcaaa
    38881 caatttttat taacaggaaa aacgttatgg ggaatgcgta tttatgatgt gatcagcttg
    38941 atcgattatt tggagacaag ggaagacaca gatactcaac ggctcgcaac gattggtctt
    39001 tctatgggag gcttattaag ctggtgggtg acagcattgg attctcgtgt caaagtttgt
    39061 attgatctag cggcacaggt ggacatcgaa atacttttgc tacatcgccg cctagatcat
    39121 catgggtttt attactatgt gccaaatcta ttgacagctt atacaacgtt agcaatccaa
    39181 gaaaaaatcg ctccgcgccc tcgtcttagc ttagttggaa aaaacgatac gatgtgcttg
    39241 gacgaaggcg tactgaagct gagaaaaggg ctggacaaaa agtatcaatc aatgggtagt
    39301 ccagaaaact tttcttcttt ttctttaaca ggcggtcata tggaaacaca agaaatgaga
    39361 aatatttgga aacaattcat aagggagcat ttatgaggat gaaagacaaa ctgataattg
    39421 gaaagaatgc tatagatacc ccgacggatt ttgaaactat ccaagaagga atcgattatt
    39481 tggcaggcct gccggatagc catccaaagg tcttgatagt aaaagaagga agatatgatg
    39541 aatatataga aagcagatta tccaatttta aacttgtggg agcaggaaca gtagttatct
    39601 ccggtaatcg ttttgcaaaa caggagtatc ttgacggtag ccagttaggg actttccgaa
    39661 gtgctacttt tttcttagaa ggatcgaatg tcgtattaga aaatcttcac atcgaaaata
    39721 cagcaggtcc aggagaaaag gtaggacagg ctgttgcttt attcaattca ggagatcgcg
    39781 tatctttaaa aaactgtcga ctatctggtt atcaggatac cttatgcacg ggtccgttgc
    39841 ctgatttgca aaaagatggt actccttttt gtacaccggc ttctgccaat cctgagtatt
    39901 gtcgtcagac ctatgaatat tgctggattg aaggaacggt ggatttcata tttggtgggg
    39961 cagatgccgt ttttcatcag tgtcatatcc atagcttagc cagtaaaaat cctggataca
    40021 tcacagcagc atctacgccg aaaaatcaaa aaacagggta tcgttttgaa agctgtacct
    40081 tgactgcaga acctgatact agaaacgtct atttaggaag accgtggcgt ccgtatgcaa
    40141 atgtcttatt tgaagcgtgt aagataggtg ggcatgttca cccaactggt tgggataatt
    40201 gggatgacaa gaaaaatgaa cagactgtca aattctctga acgtaataac aattatgaaa
    40261 catcgatcaa aagacctgaa tggatacatt tagaaaagca gggggttcag aaatgaaaag
    40321 aaaatgggaa ctattgctag gaatggtcgg tggcagtcta tctttgattt tctttggcgg
    40381 gttagcagtt actttaagta atatgtctgc gtcagagttt aaaaaaagtt atcagtcgct
    40441 tgctgtagat cattcaactt tgtctttaga aaatacattt ggattattac aggacatgac
    40501 tggtctgttt gccgttgtgt tgtttatttc acttgctttt ttagcagtag cattgttttt
    40561 gactgctaaa ggaaaatact taacaactgc cactggtttg tattttataa caggattcat
    40621 attgttgatt ggcactcagt ttattgcgtt tccttttgca tttttttatt tcgcagctgg
    40681 ggcattcagt ttatatcgag taagaatgag aaaaggagcc tgaatcatgc agactatcaa
    40741 agtgaaaaat cctattttaa gaggattcaa tccagatcct aatatattaa gagtgaaaga
    40801 gcggtattac attattgtct cctcttttga atggcttcca ggaatccgag tatatgagtc
    40861 gaaaaattta gtcaactggt cacatacaac ggatattttg accactcaag tatctttgca
    40921 aggaaatcct acaaatggaa gtatatgggc gccacaaatc agttatgctg atggattgtt
    40981 ttatcttgtt tacacagatg tcaaaagtgc aggccgtccg tttaaagatg tccacaatta
    41041 tttgatcact gcatctgaga tcacaggtcc ttggtccgaa ccaatttatt taaatagtaa
    41101 cggctttgat ccttctttgt tccatgatct tgaaacggga aaaaaatggc ttgtcaatga
    41161 actatgggat tatcgtctgg acacaccgaa taaatccagc ggtatcgtat tacaagaata
    41221 tgatgcagag aagaagaagc ttgatggtcc catctataaa atatttgcag gtacagaatt
    41281 agcgaaaacg gaggcaccac atttgtattt tcgtaacggg tattattatt tgatgacggc
    41341 agaaggcggt acaggaagag gacattcagt tacagtttgc cgagcaaaga atattactgg
    41401 tccttacgag cttgatcctg gttttcctat gctgacagct agtgacaaac ctgcttctac
    41461 attgcaatgt acaggacatg ggagcttagt acaaaccccg tcaggaagat ggtacatgac
    41521 atatctttgt accagaccat taaacggtgc ggctattttg ggaagagaaa ccgctattca
    41581 agaagtgtat tggacgtccg acgattggtt aagacttaaa gatagccaga caactccgat
    41641 ggaagaaata atgattgaga caaatgaaga ggtacaacaa gaaaaagatc atcagtttat
    41701 ggatacattt gaaggagagc taaagaagaa ctggaatgcc agacgcattt tgccaaatgc
    41761 tgattggtgt gatacaaaat gtagaccagg ttttttacgt ctgatttccg gtgaatctct
    41821 tcaatctacc ttcgaacatc atttgttagc tattcggcaa acggatttta cttttgatgc
    41881 ggagacagca tttgaataca tgcctaataa ttttaatcag atggctggac tggtactgta
    41941 cttaaatgaa tccaattata tttattgtta tgttacttgg aatgaggaca agggaaaatg
    42001 cctacgtatg atagaatcag aaaacggagt agcacaaata gaagattgga cgattccact
    42061 cacagaaaag aagacttttt tgaaagtcac tgtaaagaaa gagacagcac agtttcattt
    42121 gaaagacgta tcaactgaag aatggaagaa agtaggtccg caaaaaaaca tgtgtatact
    42181 ctcaggaggg tttactggaa attttatcgg gatagcagtg catgatctaa ataaaaaaag
    42241 aggaagttat gctgattttg agtttttcaa gtatgatgga aaagatcatc tgtaatttaa
    42301 ttctttaata aatgaggttc actaacttat tttcactagt tagtgaattt ttttgtttgt
    42361 atatattgtt atgtaaaaaa agttatcaat cttatgcatc aatgttttta gattattttt
    42421 tgttttttat ctgtcaagat aatgcaataa aaagccaaca ttgtgaatat aaaaacaaga
    42481 aaaaaagagt tataattttg aaaacgttta ctagaagagg taggaggaat tcaaatgctt
    42541 tcacaaaatg ctgttacagc aaatacaata aaaaaagaaa aaaaggaatt tgggatttcc
    42601 gataagatag gctacatgtt cggtgatcta ggtaactgtt ttattttagg tttagtcaat
    42661 agtttcttga tgatttatta tacgaatgtt cttggcgttt caggggcaat cgtaggtacg
    42721 ttattcttga tttcgagaat tttggatgcg tttgctgatg tgatggttgg aagattatgt
    42781 gatgtctcag agttaacaga aactggccga tttattcctt ggatcagaaa aatgaagtat
    42841 ccattttgct tagtgactgt cattttgttt ttgccattcg tccaacatct tccaatgacg
    42901 gtgaagattg cttatatttt tgtttcatac attgtttatg ggatttttct ctcaacaatc
    42961 aatatcccgt atggagcaat ggcagcagca attagctcaa atcctaatga ccgcacctcg
    43021 ttgtccacgt tccgaagcat tggttctgcg attggcggat caactacagg attcttcatt
    43081 ccgattttaa tgtataccca taacaagagt ggacaacaag tcgtttcagg gaatcatttc
    43141 ttttggattt caatcggatg tgctgcaata gcatttgtcg catatatgtt aacttgtaaa
    43201 ttgactactg aaagagtaag agttgtcaaa aacgaaaaag tatctgcttc tcagttggta
    43261 aaaggtttgt tagctaatcg agcactgatt attttagtca ttgttgacat tttcattgtg
    43321 atcaatcaga ttttagctgg gacgaatatg acttatttat tcaatgatta cttccataat
    43381 aaacaagcaa tgtctgttgc tcttttattt acttacggaa cagtagtagt tttagcacca
    43441 tttgctacaa agctgacaaa attatttggt aaaaaggaag caagcgtatt tgcgttactt
    43501 gcttcttcat gtatgtactt gctgatgtat ttccttcata taacaaatgc atgggtatat
    43561 ctgattcttt tatttatcgc tactttagga tctggtttat tcaatttgat ggtctgggca
    43621 ttcattactg acgtgattga ttatcatcaa tataagacag gcttaagaga agatggaaca
    43681 gtatatggag taaattcttt tgcgagaaaa gtaggacaag catgtgcagg agcggtaggc
    43741 ggatttatgc tggcactcat tggttaccaa tcgtctacaa caggtggagc catccaatca
    43801 gttttagtac aagaaaggat ttatacgatg gcaaacttgt taccagcatt ctgcttattt
    43861 gtatccgcaa tcattttgct agtaggttac ccactgaatc gttcaaaaac tgtatttatt
    43921 ggtgaagaat tgaaaaaaat caatgaaaga tagggagaaa cgtattgcgg acaaaaaaag
    43981 ggattatcaa tgctttgaac tttaaaacaa acgacactca acaagatgat acaataatac
    44041 tgcaaaaagc aatcgatcaa ggtgcgaaag aacacttacc tgtatttatt ccaaaaggca
    44101 tttatttagt cggagccctt tttttaaaag ataagagtca tcttatcttt gaagaaggtg
    44161 cagtcctcaa ggggagaaca gatatcgaag catttccaga aatcgataca cgagtagccg
    44221 gcgtagaaat gaaatggcca gcagcgattt tgaatgttct ctcggcgaaa gatattctta
    44281 tagaaggaaa aggaataatc gacggtcaag gagaccattg gtgggagctg tattggggaa
    44341 aagatcaaaa aagcgggaca agagctgaat acgatcaaaa gggacttcgt tggattgcgg
    44401 attatgctat taaacgtcca cgcgcttgtt tactgtatca tgcagaacat gttgttgttc
    44461 gtgatttgat ttttcagaaa tcaggttttt ggaacttgca aatcacttat tctaacgatg
    44521 tattagtaga aaaagtaatc atccgcaaca atgatggacc aagtacagat gggattgata
    44581 ttgattcttc aacaaatgtg cgtgtctatg aatgtgattt ggcctgtggt gatgattgta
    44641 ttgctattaa gtctggcaga gatggcaatg gtgcaagagt caatcgaaaa tcatcaagaa
    44701 tcgaggttgc tagatgtaaa atccgttcag gatacggtgt aactattggt agcgaagtta
    44761 gtgctggagt tagtgacgta tatattcacg atatcgattt ttttcagtca gactgtggta
    44821 ttcgaatgaa atcttcaaaa gaaagagggg gagtgatcga aaatatcagg gtagaaaatc
    44881 tgaatatgat tgatgtacaa tttccttttt cttggataat ggactggcat aatgaataca
    44941 atagaaaaaa tagcaatgac cttgctcaaa tgccagaaag ttggcaagca gtggctgaag
    45001 aaatacctga atcaaagcaa atgacgaaag tcaaagacat acacatcaaa aacgtacagg
    45061 catgcctttc tccaggttac tcacttcctt cccgagcttt tgatttagta gcatttcctg
    45121 aaaagcctat agaagatgtc tgcttttctc attgccaaat aactgcaaaa gaattcgggc
    45181 gaatcgaagc agtacaaaac ttatgtttca aaagctgtga tttatctata gaaactggca
    45241 acacagctgc aaacaatact tttgacaatc gttgaaatta taataaggtc aagcttaaat
    45301 aatattttag cttgaccttt atttttttta atgatatatt aaacaacgtt gtcgcgcttc
    45361 attgaaacat tttatgaaaa ttccaaaaaa gagttgacac atgtttttag acgtgataag
    45421 ataactaagt cgttaaaaag aaccacttgc gaagcttgat tctatcgaaa aaaacttcaa
    45481 aaaagttctt gacaaataac aaacgataag ttatgataaa tgagttgctg aaagatttcg
    45541 aaaaacgaaa
//
LOCUS       DAZAFF010000010        42805 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000010 DAZAFF010000000
VERSION     DAZAFF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.010"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..306
                     /locus_tag="ACG9BB_000573"
     CDS             61..306
                     /locus_tag="ACG9BB_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFN2836545.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            399..845
                     /locus_tag="ACG9BB_000574"
     CDS             399..845
                     /locus_tag="ACG9BB_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2836546.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(904..1365)
                     /gene="mscL"
                     /locus_tag="ACG9BB_000575"
     CDS             complement(904..1365)
                     /gene="mscL"
                     /locus_tag="ACG9BB_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFN2836547.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            1617..2885
                     /locus_tag="ACG9BB_000576"
     CDS             1617..2885
                     /locus_tag="ACG9BB_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFN2836548.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2878..4164
                     /locus_tag="ACG9BB_000577"
     CDS             2878..4164
                     /locus_tag="ACG9BB_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFN2836549.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            4903..5826
                     /locus_tag="ACG9BB_000578"
     CDS             4903..5826
                     /locus_tag="ACG9BB_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFN2836550.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            6035..6613
                     /gene="pgsA"
                     /locus_tag="ACG9BB_000579"
     CDS             6035..6613
                     /gene="pgsA"
                     /locus_tag="ACG9BB_000579"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFN2836551.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            6853..8094
                     /locus_tag="ACG9BB_000580"
     CDS             6853..8094
                     /locus_tag="ACG9BB_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFN2836552.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            8203..9252
                     /gene="recA"
                     /locus_tag="ACG9BB_000581"
     CDS             8203..9252
                     /gene="recA"
                     /locus_tag="ACG9BB_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFN2836553.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            9663..11219
                     /gene="rny"
                     /locus_tag="ACG9BB_000582"
     CDS             9663..11219
                     /gene="rny"
                     /locus_tag="ACG9BB_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFN2836554.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(11298..12104)
                     /gene="proC"
                     /locus_tag="ACG9BB_000583"
     CDS             complement(11298..12104)
                     /gene="proC"
                     /locus_tag="ACG9BB_000583"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFN2836555.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            12245..14818
                     /gene="mprF"
                     /locus_tag="ACG9BB_000584"
     CDS             12245..14818
                     /gene="mprF"
                     /locus_tag="ACG9BB_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFN2836556.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            14972..15823
                     /gene="ispE"
                     /locus_tag="ACG9BB_000585"
     CDS             14972..15823
                     /gene="ispE"
                     /locus_tag="ACG9BB_000585"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFN2836557.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            16068..17006
                     /locus_tag="ACG9BB_000586"
     CDS             16068..17006
                     /locus_tag="ACG9BB_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFN2836558.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            17029..17709
                     /locus_tag="ACG9BB_000587"
     CDS             17029..17709
                     /locus_tag="ACG9BB_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN2836559.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            17682..18506
                     /locus_tag="ACG9BB_000588"
     CDS             17682..18506
                     /locus_tag="ACG9BB_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFN2836560.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            18673..19491
                     /gene="purR"
                     /locus_tag="ACG9BB_000589"
     CDS             18673..19491
                     /gene="purR"
                     /locus_tag="ACG9BB_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFN2836561.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            19994..21367
                     /gene="glmU"
                     /locus_tag="ACG9BB_000590"
     CDS             19994..21367
                     /gene="glmU"
                     /locus_tag="ACG9BB_000590"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFN2836562.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            21461..22432
                     /locus_tag="ACG9BB_000591"
     CDS             21461..22432
                     /locus_tag="ACG9BB_000591"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFN2836563.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      22438..22540
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22606..23175
                     /gene="thiT"
                     /locus_tag="ACG9BB_000592"
     CDS             22606..23175
                     /gene="thiT"
                     /locus_tag="ACG9BB_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFN2836564.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(23256..24029)
                     /locus_tag="ACG9BB_000593"
     CDS             complement(23256..24029)
                     /locus_tag="ACG9BB_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836565.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            24296..25996
                     /locus_tag="ACG9BB_000594"
     CDS             24296..25996
                     /locus_tag="ACG9BB_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFN2836566.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            25966..26184
                     /locus_tag="ACG9BB_000595"
     CDS             25966..26184
                     /locus_tag="ACG9BB_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFN2836567.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            26204..27094
                     /locus_tag="ACG9BB_000596"
     CDS             26204..27094
                     /locus_tag="ACG9BB_000596"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFN2836568.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            27112..27396
                     /locus_tag="ACG9BB_000597"
     CDS             27112..27396
                     /locus_tag="ACG9BB_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836569.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            27883..28497
                     /gene="gmk"
                     /locus_tag="ACG9BB_000598"
     CDS             27883..28497
                     /gene="gmk"
                     /locus_tag="ACG9BB_000598"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFN2836570.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            28502..28807
                     /gene="rpoZ"
                     /locus_tag="ACG9BB_000599"
     CDS             28502..28807
                     /gene="rpoZ"
                     /locus_tag="ACG9BB_000599"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN2836571.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            29060..31468
                     /gene="priA"
                     /locus_tag="ACG9BB_000600"
     CDS             29060..31468
                     /gene="priA"
                     /locus_tag="ACG9BB_000600"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFN2836572.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            31483..31974
                     /gene="def"
                     /locus_tag="ACG9BB_000601"
     CDS             31483..31974
                     /gene="def"
                     /locus_tag="ACG9BB_000601"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFN2836573.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            31967..32905
                     /gene="fmt"
                     /locus_tag="ACG9BB_000602"
     CDS             31967..32905
                     /gene="fmt"
                     /locus_tag="ACG9BB_000602"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFN2836574.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            32905..34263
                     /gene="rsmB"
                     /locus_tag="ACG9BB_000603"
     CDS             32905..34263
                     /gene="rsmB"
                     /locus_tag="ACG9BB_000603"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFN2836575.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            34276..35016
                     /locus_tag="ACG9BB_000604"
     CDS             34276..35016
                     /locus_tag="ACG9BB_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFN2836576.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            35013..37082
                     /gene="pknB"
                     /locus_tag="ACG9BB_000605"
     CDS             35013..37082
                     /gene="pknB"
                     /locus_tag="ACG9BB_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFN2836577.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            37247..38146
                     /gene="rsgA"
                     /locus_tag="ACG9BB_000606"
     CDS             37247..38146
                     /gene="rsgA"
                     /locus_tag="ACG9BB_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFN2836578.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            38159..38809
                     /gene="rpe"
                     /locus_tag="ACG9BB_000607"
     CDS             38159..38809
                     /gene="rpe"
                     /locus_tag="ACG9BB_000607"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFN2836579.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            38810..39448
                     /locus_tag="ACG9BB_000608"
     CDS             38810..39448
                     /locus_tag="ACG9BB_000608"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFN2836580.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            39603..40457
                     /gene="mreC"
                     /locus_tag="ACG9BB_000609"
     CDS             39603..40457
                     /gene="mreC"
                     /locus_tag="ACG9BB_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFN2836581.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            40461..40970
                     /gene="mreD"
                     /locus_tag="ACG9BB_000610"
     CDS             40461..40970
                     /gene="mreD"
                     /locus_tag="ACG9BB_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFN2836582.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            41225..42799
                     /locus_tag="ACG9BB_000611"
     CDS             41225..42799
                     /locus_tag="ACG9BB_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFN2836583.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT        14118 a         7636 c         9258 g        11793 t
ORIGIN      
        1 ttatggtaaa cttgaaaatt ttaaaaatat gtggtatagt tgatagtaga ggtgaactgt
       61 atgccaacaa ctttagcttc aatcaagaaa gatttagaag gtcgtatcgg cagcccaatc
      121 atgttggttg ctcagacagg aagaaagcgt caaacagaac gccgcggcgt tttaacagaa
      181 acctatcctt cagtattcgt tgttgatttg gatcctgaag aaaattcttt tgaacgagta
      241 tcttacagct attcagatgt acttactcgg acagttgaga tcgaattttt aactgaagca
      301 gtttaaaaag caaacgagcc tgagattcgt aaggttcgtt tgcttttttg tgtgcgaatc
      361 ggtatgctaa gaataaaaaa gcagggggcg tttgcttagt ggaatatcag gtaaagaaat
      421 ttgaagaatt gtcaacagca gagttttacg cgatcgtcaa agaacgaatc gcagtttttg
      481 ttgtggaaca acagtgtcct tatcaagaag ttgatgacat tgatggagaa gcttggcata
      541 cgttttttca agatactgat gggaagatcc ttgcctacac gcgagtatat gaagaagcgg
      601 agagtatcca ttttggacgt gtccttgttc atcaggacaa ccgtaaagat ggtttaggaa
      661 gaaagattgt tcaagaaacg attaacatga tccagaaaaa ttttccagaa aaaccaatcg
      721 tgatcggcgc acaagaatac ctacaagctt tttatgagtc gtttggattc aaggcaatct
      781 cagatgtgta tttggaagat gatattccac atatcgacat gaaaaaggaa caagtagcaa
      841 gttaagaatc taaaaaaaga aagccgacac tgaaaatcgg ctttcttttt tttagattct
      901 ttattattta gaagtatcgg aaagatttgt ttgctctact ggatgtgtat tgtcttctgc
      961 cacttgtttg gcaagaagat cgcgtatgtc actaagataa tcttctgcag taggagtttc
     1021 ttccacttcc acttgctctt ctttggagcg taaattcttc gcacggtttg ccgctttgac
     1081 aactaagaat aagacaaaac cagtgatcag aaatgtaatg atagcactaa taacgttacc
     1141 aaacgcaaat gtgacgcctt ttgtcggcga ccagtcaaga attgaaagtg ctccttctaa
     1201 atcagaagta cctgtgatca ttgcgacaaa ccaacctatt agtggtgtga tcaagccttg
     1261 caccacttgg tttacgatgc ttgtaaacgc actaccaata acaacgccga cagcaagatc
     1321 caagacatct ccacgcatga tgaactcttt gaattctttg atcatgaatg accctccctt
     1381 cttttatttg tctttagtat atcgaaaaat aaaaaataaa aaaacaatat acgcttttag
     1441 ccgtctgttt cttttgataa aaaacatcta tgctatacta taaagtgtaa catcatttag
     1501 aaaaaacggc gaattgcctg aacttataaa tttcaagcag aggaataagt aaaaaaggca
     1561 ggttgaccca ttaaaagagt aagcgggcaa tcaactgtga aaaggagcat ataaatatgg
     1621 tgtatcaatt ggcagaaggc gtgaatcttc atgtcttacc aacaaaacaa tacaaaacga
     1681 tccggatttt tatccgtttt actgcgcgtc tgcaacaaga agtcatcacg aaacgttcac
     1741 ttctcagcag catgttggaa acaaatagtt tgaattattc ggatcagaca aagttaagtg
     1801 cgcaacttgc agagctttat ggcgcaagct ttggattatc agttagaaaa aaaggaaatt
     1861 tacattggct aaacgcaggg atcagttttg tgaatggtga atatgtcaat gatccaaacc
     1921 ttttttctca agcggtagat tttttaaaag aggttttatt ttatccaaat attaaaaatc
     1981 atcagtttga tcagttgacg tttgacttgg aaaagaacaa tctgcgtcta tatttggaaa
     2041 gtttgaagga agataaacaa acttttgctt cctatgcctt gcaagaactt tattttgaaa
     2101 acagcccgga acaaaagatc ccagggttgg gcgtagtcga agatttggac aagatcactg
     2161 ctagttcgct agcgtcctac tatcaagaaa tgatggcaaa tgatcaaatc gacatttttg
     2221 ttgtcggcga tgttgatccg gacaaagcag ctgaagcagt tgggcaatta ccatttgagt
     2281 cgcgggaaac agtttatcca gatctgtttt atacccaacc tcaagtgaat atcgtgaagg
     2341 aacgacaagt acgtgagcca atcgttcaag caaagctgaa cctagcttat cagacaaatg
     2401 tgtattacga tgaaccagaa cgttttgcgc taatggtctt taacggttta tttggcggtt
     2461 ttcctcactc taaacttttc atgaatgtca gagagaaaga aagtctagct tattatgcct
     2521 ctagtagcgt ggatacgttc cgaggattca tgagtgtcca aaccggtatc gatgaaaaga
     2581 accgaaatca agtacttcat ctgattcacg agcaattaga aagtttgcga aatggtgaaa
     2641 taacagagtt ggaactgtct cagacaaaag caatgctgag aaaccaatat ctgctttctc
     2701 tagactctcc gcaagcggca atcgaagctt cttttttgga cagctggctg ccggaaacaa
     2761 aattatctga tgaagaatgg ttgaaacgta tggagagcgt aacgatcaaa gagatacaac
     2821 aagtagctga gcaaatcgaa ttgcaggcaa tcttcttttt agcaggagga aatgcaaatg
     2881 aataaaacag aatatgagca aatcaatgaa acactttatc atgaagtact gccaaacggt
     2941 ttgaccgtgt atttgttgcc taaaaatgac tatcacaaga catatggttt gttcagtacg
     3001 aattatggtt cgatcgataa cgaatttatt ccctatggtg aaaaagaaaa ggtgaaagtt
     3061 ccagatggaa ttgcccattt tttagaacat aagttgtttg aaaaagaaga tggtgatgtc
     3121 tttcaattat ttgggaaaca aggagcttcc gccaatgctt tcacaagttt tacgaaaacc
     3181 agctatctgt tttctacgac ggatcaggta gaaaagaatc tgacgactct gatcgatttt
     3241 gtacaagcac cttacttcac agaagaaact gtaaacaagg aaaaaggaat catcggtcag
     3301 gaaatccaaa tgtatgaaga cgaccctaac tggcggatgt tttttggcat actaaataac
     3361 ttgtatccta cacacccatt gcatatcgat attgcgggca cggttgaaag tatcgacaaa
     3421 atcacagctc aagacttgta tacgtgctat cggacgtttt atcagccaag caatatggtt
     3481 ttatttgtag ttggtaagat ggagcctgag aaattgatga aacttattcg ggaaaaccaa
     3541 gaagcaaaaa atttccctcc aaagcaagaa attgtacgtt atttccctga aaacacaaaa
     3601 gaaatcatca aacaaagtgc attggaagca gccattacac gagataaatt tgtacttggt
     3661 attaaaggat tagacacatt gccgcaagaa ggaacagaac tgttacgcta taaaacagca
     3721 atcaatctgt tgttccaaat gattttaggc aatacttctc gaaactatct agcgatgtac
     3781 aatcaaggaa ttatcgatga cagctttggt tttgaattca gcttggatcg tgaatttcac
     3841 tttgcggact tcagcggaga tacggacgaa ccagagaaag cagcagaaaa agtcaaagaa
     3901 atcattcttg gattcgctga tgatccggaa gtgtcagaaa caaatctcga tttgctgaag
     3961 aagaaaatgc ttggacaata tttccaatca ttaaattcga tcgaatatat tgccaatcaa
     4021 tttacgcaaa gtttatttgg cgatcgaact ttatttgatc ttcctgaaat catcgattcg
     4081 atccaaatga aagacgtact ggcagctggg gaagcattta taagtgaaga ggccttcagc
     4141 gagttttaca tgagaccaaa ataaaccagt attttatgag gaaaagtctt aaatttcgtt
     4201 ttagacttgt agtaggaacc tcctccaaaa tcagctttta tggtattctt agttgtgttg
     4261 gaaaaattag tgagaaaata aaacaaatca tgctctcttt taatcaactt atattaaaat
     4321 aaaatcaaac gaagtaatca aagaggttgt gacagaagtg gtcagcttca agaaataaga
     4381 aggaatttac gaaaattgct tctcaaattt ttgtgatatt tcggcttatt gccgaagaag
     4441 ctacttctgg aacaccgtgt accaagaggt tgtgaaaaag ctgccctgca tcaagtaata
     4501 agaacagcca agtaaaaaca gcttcttaat gtttttaact tggttggact tattaccgca
     4561 gatgcaagct tttgaacacc gtttaaggaa gaggttgtga aatcagcgtt ttgacctgag
     4621 tattagagaa acatacggaa aaatatctcc ttatattttg ccggtttttg agctaatacc
     4681 gaggaacagc tgattgaaca ccgtttaaga aagaggtcgt gagcctgccg tttaactccg
     4741 agtcacaagg aacaagttca aaaaacagct tcttatattt attggatatt gtgacttgtg
     4801 accgaggagt tggcaggaga acaatgttta ttcggaaaaa aggagctgtg acatgacttt
     4861 tgtcacagca cccaaacata acgtctttcg ttgcaacagc agatgcttcg gagaatcaaa
     4921 cagactggag aagatgaacg agtggctttt gaaaccatcg gagaaaaatt acggcaagca
     4981 cgcctgaata aaaaaatatc tttagatgaa ttacagcaga ttacaaaaat tcaaaaacgt
     5041 tatttggaag caatcgacaa tgatgatttt gaccaattgc ctggtaaatt ttacgtgcgg
     5101 gcatttattc ggcaatacgc tgaagctgta ggagaagatg gagaccattt agtcgatgtt
     5161 tttgatggga aaagcaaatt gacaggatcg gtccctgaac gtccgcagcc tgaaacagta
     5221 agtacttcta gaaaagcaat gcatcaggaa gaatcgcatc caagtcgctt ttggacaagt
     5281 cttcctgtca ttttattagg attggttgct ttggcgattg tcgttgtcgt gggctatatg
     5341 acttggcaag atcgtcaagc tgatccaata atcggagaca cagcttcttc gcttaaagta
     5401 gaaggatcag tcgaggagtc ctcttcctca gagcaaagca cgcagaccaa agagtcttct
     5461 acagtagaat caacaacaaa atcaagtgaa gaaaagaaaa tggcaatagc tacaggagaa
     5521 gattctggca gtgccatcgc agtaaatatc acagacgcta ccaagcctgt aacccttgaa
     5581 tttactgctg tcaatcgtgt atggttaggt gtgctagtag acaatgccta tgtttatcaa
     5641 gggacactgg cagcaaatga aacacagagt accgtgttac cggaaacagc aacaaatgcg
     5701 accatcacga ttggtgcagc aagcaatgca acgatcaaag cgaatggaga atccgtacca
     5761 gttaatccag gggaaaacaa tcagagtcca aaaaatgtga accttactct tcaatacgca
     5821 gaatgatcgt ataaattagt ttacgcatct gtttacttgt aggtataaaa aagaaggatc
     5881 gtgacaaatc tgtgtcgcgg tccttcttcg ttaattcaaa aagatcaatt tcaataaaaa
     5941 tactcctttg aataaattca tttggaaaag taaataatat cggtggaaac ttcgctgata
     6001 gtcacgtaga atagggaaga aaggagtcga tagtgtgaat ttaccaaata aattaactgt
     6061 acttagaatt tgtatgatcc cgattttcat tttggttgta ggtttacctt tagattgggg
     6121 acaatttaac gtaggagaca ccacactggc tgtcacacag ctagtaggtg cagttatttt
     6181 tgctttagct agtatcacag actggttaga tggaaaaatt gcccgtgcgc gaggccttgt
     6241 cactaatttt ggcaagtttg cggacccgtt ggcagataaa atgctagtta tgactgcttt
     6301 tatcatgttg gttgaccaag gaaaggcccc tggttgggta gtagcgatca ttgtctgtcg
     6361 tgaattagcc gtcacaggct tgcgtttact acttgtcgaa catggagaag tcatggctgc
     6421 agcttggccg ggaaaagtca agacggctac acaaatggtt gctattatct tgctgtttat
     6481 caataatatc ccattcagcc tgatcggtat tccagtagat caaatcatgc tttatgcttg
     6541 tctgatcttt actatctatt caggtgtgga ttacttcatc aaaaacagcg gtgtgttcaa
     6601 aggatcaatg taagtatcgt ttagtggaga ggttgtgagc ctgccgtcta actctgagtc
     6661 acaagggata atttcaaaaa acagcttttc atgttttatg tatattgtga cttatgaccg
     6721 aggttttggc aggagaacac tgtttatttg gaacaaggag ctgtgacaag atttttgtca
     6781 cagctccttg ccctcttttt aaataaaaaa atataaaaat acagcgtata ctatataagg
     6841 gagcgggaag taatgaaagc agaaataata gctgtcggaa cagaactttt actaggacaa
     6901 gtcgtgaata cgaatgccac tttcttgtca gaacagctag ctgatctagg tatcgaagtt
     6961 tattatcaaa cagttgtagg agataatcag cagcgtttag aagagttgat tgctctagct
     7021 gaaacaagaa gtgaactcat actcttatgt ggcggattag gaccgacaga agatgacctg
     7081 acaaaagaag caactgcggc tcacctcggt aagtctctca tacaaaatac ggaaggatac
     7141 aaaaaattat tggcatattt tgaaacaacc catcgaaaaa tgaccaaaaa caatctgcaa
     7201 cagtcccaga ttatcgaagg cggggttcct ctaccaaatc gtacagggct tgctttaggg
     7261 actttttatc aaacagatac acatgcgtat atccttcttc ccggtccgcc taatgagttg
     7321 aaaccaatgt ttgtggaaca agtacgtcct ttactagagg aacgttttcc ttctgaagaa
     7381 aaactaattt ctaaagtttt gcgcttttat gggattggtg aatcgcgact ggtcactgag
     7441 ctaaaagatc tgattgagac acagatcaat ccaacgatcg caccatatgc caaaccaaat
     7501 gaagtgacat tacgactgac agtgaaaaca aatgatgttc aggcagggaa tcaagctctt
     7561 ttagcattag aagaaaaaat ccaagaacga gtaggggaat atttttatgg atacggcgat
     7621 gataattcgc tagcaaaagt cgtagtggaa ctactgaaag aaaacaagca gactgtaaca
     7681 gcagcggaaa gtttgacagc cggcgccttc caagccgcat taggcgacat tgctggtgta
     7741 tctgaagtat ttccaggcgg attcgttacc tactcgctcc aaacaaaagc tggttttctg
     7801 gagattgatc cggagctatt agcagaatat ggaacagtca gcaaagaatg tgtggagcag
     7861 atggccattc aagcacgtga gatagcaaga acagattaca gtgtagcttt caccggagtg
     7921 gctggaccgg acacgttaga aggtcaatct gcaggaacag tatggatcgc tatttcatct
     7981 aaagacggcg tggtttcgga aaaatatcac tttgccagag atcgtgctgc tatcagatat
     8041 aatgcggtta tgcaaggatt tgatcttttg agaaaacagc ttttaagaaa atgataaatg
     8101 cgaatatttg ttcggttttt tcttgctttt tttcactaaa aaagatatga taaggataat
     8161 gaaatgttgc tctgatttgt aaatataagg aggattatcg tattggcaga tgatcgtaaa
     8221 gcagccttag atgctgcact gaaaaaaatt gaaaagagct atggtaaagg ctctattatg
     8281 aaattgggcg aaaaaatcga ccaacaaatc tctacaattc caagtggctc tcttgcgtta
     8341 gatgtcgctt taggtgtcgg cggttacccc cgtggacgta tcatcgaagt atatggacct
     8401 gaaagttcag gtaaaacaac agttgcacta cacgctattg cagaagtaca aaaaaatggc
     8461 ggaacggccg ctttcattga tgctgagcat gcgttagatc cgcaatatgc acaaaaatta
     8521 ggtgtgaata tcgatgaact acttctttca cagcctgaca caggagaaca aggtctagag
     8581 atcgctgatg ctttagtatc aagtggggct gtagatatcg tagtagtcga ttcagttgct
     8641 gctttagttc cacgagcaga aatcgacggc gaaatgggtg actcacatgt cgggttacaa
     8701 gcacgtttga tgtctcaagc attgcgtaaa ctctctggtt cgatcaacaa aacaaaaaca
     8761 atcgctattt tcatcaacca aatccgtgaa aaagtaggcg tgatgtttgg gaatccagaa
     8821 atcactcctg gaggacgcgc attgaaattc tacgcaacga tccgtttgga agtacgtcgt
     8881 gcagaacaat tgaaacaagg tacagatatt gttggtaacc gtacaaagat caaagtcgta
     8941 aaaaacaaag tagcacctcc tttcaaaatc gctgaagtag atgtcatgta tggtttaggg
     9001 atctcacaag aaggagaact tctagacatg gcagtagaaa aagacattgt tgataaaagc
     9061 ggtgcttggt attcttataa agaagatcgg attggtcaag gacgggaaaa cgctaaaatc
     9121 tatatggcta atcatcctga aatgatggca gaagtttctg ctctagttag agcagcttat
     9181 ggtattggtg aagaagtcgc tgtcccagaa gatgaaaaag gacaagaaga attgccttta
     9241 gtagaagaat aaaaattatg ataaacaaaa acggctggtc tgaagagtag gtactctcct
     9301 tgacctgccg tttttttatt gaataaagaa atcgtgagtt atttaattat acgagtgggt
     9361 acataaaata aaaatatctg ttcatctaca aagaataatt taatagaaaa gtttctcaat
     9421 ttaaaggttt acctatatga aactagtgtc atttatttta aaaaccatcg tttaatgaat
     9481 aagtagaaag ccgtttcttt gtttatttaa agttgacact acggggtaca aacattagaa
     9541 taaagttgtg catgtttttt gtacacatat atgcgaagat gcatattgac gagtttagaa
     9601 taatttatca tagaagctgt atagatgata aaaatgaaaa agatagtatg gaggtggatt
     9661 ttatggtatt caacattctc ctcgctatca tcggtttaat tgtcggtctt ggtttagggt
     9721 ttatggtcgc aaagtcacgt cacgagaaag aaatagctgg tgcacaatca tcagctgctg
     9781 gaatcatcga tagtgcaaaa aaagaagcag aaactctaaa aaaagaagca ttgttagaag
     9841 ctaaggaaga aaatcagaaa taccgttctg aagttgaaag tgagctgaga gaaagtaagt
     9901 tagaattaaa atctcaagag aatcgtttga ttcaacgcga acaaacgctg aatcgtaaag
     9961 atgactcgtt agaaaaacgc gagaactcac ttgaaagtaa agaagaagca cttagttcaa
    10021 aacagcaatt aattgaagag cgagaaaaag acgttgaaaa aatgattgaa gatcaacaaa
    10081 aagaactaga aaaaattgct ggtttatcaa aagaggacgc acgagatgtc atcatgaaat
    10141 cgactgaaga agagctgaat catgagttaa ctctaatggt aaaagaatct gaacagcgtg
    10201 ccaaagaaga agcagatcgc aaagcgaaaa acttattatc attagcgatt caacgctgcg
    10261 cagcagatca agtttcagaa acgactgttt cagtcgttac ccttccgaat gatgaaatga
    10321 aaggtcgtat cattggacga gaaggtcgta atattcgaac gcttgagacg ctgactggta
    10381 tcgatttaat catcgatgat acaccggaag cagttgttct ttccggattc gatccaatcc
    10441 gaagagaaat cgcacggatg accttggaaa aactgataca agatggacga atccatccag
    10501 cgcgtatcga agaaatggtc gagaaatctc gtaaagaaat ggatgaacgt attcgcgaat
    10561 acggggaaga ggcagcgttt gaagtcggtg ctcatacact tcatccagat ctaatcaaga
    10621 tcatcggacg tctgcgattc agaactagtt atggacaaaa cgtcttgaag cattcggtcg
    10681 aggttgcaaa attagctggt atcttagcag cagagcttgg cgaagatatc caattagcaa
    10741 aacgtgccgg gttgctccat gatattggga aagcacttga ccatgagatt gaaggatctc
    10801 acgttgagat cggtgcggaa ttagctgcca agtacaaaga aaacccagta gtaatcaacg
    10861 cgatcgcttc tcatcatgga gatgtggaag ctacttctgt tatctctgtc cttgtagctg
    10921 cagcagatgc gctctcagca gcaagacctg gtgcgcgaag tgaatcatta gaaaactaca
    10981 tccgtcgttt aagaagtctg gaaaatattt cgaacagctt cgccggtgta gaatcaagtt
    11041 acgcagttca agctggacgc gaagttcggg taatggtcaa gccagaagaa atctcggatt
    11101 tagacgctgt tcgtttagta cgtgatattc gtaaacgaat cgaagaagaa ctggattatc
    11161 ctggacacat caaagtaatc gttattcggg aaactcgtgc aactgattat gctaaataat
    11221 aaaaaataca aaagaagagc cgcggcaaaa gccacagctc ttcttttttt gtaaggcacg
    11281 aaaaaacatt gctatcatta ttttttattc aacatctcaa gatctttctc gatcgtcgta
    11341 ttgattcctt tgatgattgc tgggatgaat cctgcttctt ctaaggcaac tacacctgca
    11401 acagttgtac cgccaggaga agaaacttgg tctacaagtt cccatggatt tttatcactt
    11461 gaaaggaatt tttgacaaga acccataatt gcttttgcag caatctcagt ggctgtctgt
    11521 ttggacaaac cattcagtac accagtccgt cccatcgcat cgatcagcat gtaaacgaat
    11581 gcgggtgagc tgccagcaag gccaatgaat gtactgaaat ctgattctgc taaaggatag
    11641 acgctgccga ttgcttcaaa aatcatcgta gcttgctgaa gctgttcctc cgtggcagct
    11701 tcatttttgc aaatcgcaga aacgcctgct cctacgctga cattgacatt cggcatcaca
    11761 cgtacgatgg aggcctgagg atcttcaagc gctgtttcta aatctattaa tgattttcct
    11821 gaagcaatag aaatgaataa gactttctgg ctatattctc ttagttcttc caatactttt
    11881 tcaatgattt ttggtttcac ggctaaaaga acgatatctg attgtgatgc aacttcctga
    11941 ttacttttgc atgcatggac gccagtttca gcagcaaatt tttgtgcatt ttcaaaatgt
    12001 ccactggaaa cgaacacctc ttctttcgag actgttcctt tttttaataa accatcaata
    12061 atagcttgtg ccatattccc tgtgccgata aatcctactt tcatgttatc ctctccttta
    12121 gcattaggat atctttcttt ttctatcatc gcaagcaaag aacaggtcta aagtggaaaa
    12181 attaaaataa ataagataac attggggtat gacacaaaag tatacaagtt aggaatggga
    12241 aaatgtgaaa aataaacgat acttgatgct gcaatggata aaggaacata gtctgatttt
    12301 aaaactgatt tttttcggct ccgtgttagt ctttgtggca aatcaagtcg caaatattgc
    12361 taatggcatg agttggcaag atatttggca aaggatggga caacaaagcc gcatgacgat
    12421 cctgctcatg attctggctg gactgctggg tgtcactccg atgttgttgt atgattgggt
    12481 gacgatcaga gtcttagaaa aacaaggaaa accaaaaatg gaaagaaaag agtttcttct
    12541 tgctgcgtgg gtaacaaaca cgatcaacaa tctcgccggt tttggtggtg tggtaggagc
    12601 aagtctgcgg gctagttttt atggaaagca gacaaataga aaaatggttt tagccactgt
    12661 atcaaaagtc gcactattta tgctgactgg tttgtctgta tggtcgtttc ttacatttat
    12721 tgatgtcttt tttattcagt cagaaagtat ttttcgaagc tattggatct ggcttttcgg
    12781 cggtagtttt ttagcaccag tcattttctt ttttgcttat ctaaagagaa agacattgtt
    12841 cttcgagttt tatccaaaag gcttgcttgg tctgctactg gcatcctttg gacaatggac
    12901 gggagccctt gcggtatttt tgatcatcgg caagttgatg agaatagata tttcgatgct
    12961 ttcgatctat cctatgttca tgattgctac attgatagga atgctgacga tggtcccagg
    13021 tggaatgggg acttttgacg ttttgatgat cttaggaatg agtcaattgt ctgttcggca
    13081 agatgtagca gtagtttggc tattatatta tcggttgttt tattatgttc taccttttat
    13141 gacgggactt ctattgttca tcacacatac aggagtcaaa atcaatcgat ttttagataa
    13201 cattccgcga ctgtttttcc aaaaggttgc gcatatcgtg ctcgtaagtg ctgtctattt
    13261 tgcggggatc atgatggtac ttctatcaac aatcactaat ctgtctgcaa tcagtcggct
    13321 atttcaagta ttattgccgt tctcattcaa ttttttagat cagacagtga atctattggt
    13381 tggcttttta ttactcggcc ttgctagagc actttcagta aaagtcaaaa aggcatttct
    13441 gcctacgatt gctttactat cttttggtat tttaaataca atcacacgta cgttatcttg
    13501 gcagttgata ttggtatatg tcttgattct gttagctgtg tggttatcca gaaaagaatt
    13561 ttatcgaaca aaattcgttt attcttgggg agctataatc tttgatgcta gcttgttcgg
    13621 ctttttgttt atcgtttacg caattgcagg ctatcacagc ggacgctttt tagtcggtcg
    13681 aataacagga aatcatttta ttttgtttcc ttcagatgat gtgtggttct cagggcttat
    13741 tggtttgggc atctcattgc tagctttagt ggcgctttat caatatttag ctgaagatac
    13801 tacgaagctt ggcagtgagt acgaaaaaga ccgttttgac cgtttattgg aaacatacgg
    13861 aggcacagct acaagccatc agcttcgctt gtcaggttac agttattttt attaccaaga
    13921 agacgaccaa gatcaagtcg tttttggtta tcagatcaaa ggaaacaaat gctttgtttt
    13981 aggagatcca gttggcaatc ctaaaatgat gcgggcagca acgaatcagt ttatgaaaca
    14041 agcagattgt ttaggctatc agcttgcgtt ttataagata tcagaaagct acgtagtgat
    14101 gcttcatgaa ttgggttttc acttcacgaa agtaggtgaa gcagggattg ttgatctagc
    14161 aacagaagac ttgtctcggc tgagtcagca acttgaatat caacgtttga tgaaagaagg
    14221 ataccatttt acttggtacg atcaactgcc ggaagaatta catcaagctg taaatgaagt
    14281 ttcacaaaaa tggcttcaag gtgcacgaga aaaatacttc tctgtgggac gatttgataa
    14341 atcatatgta ttatctagcg gtgtcggtat tgtccgtaaa gatcaaaaag tcgttggctt
    14401 catcaccgct cagccgatga cgaagaagca ggcgggatat gatttgttgc gggtcttacc
    14461 taaagaatct gaggaattag ctgactatct gttagccaat ctgtttattg tatacgagaa
    14521 aatgggctac tcggaagcca atctaggctt agctccatta gctaatgttg gagaaacaga
    14581 tttttctttc tttcaagaaa aagtcatgca tatcgtatat aattatggta attttttcta
    14641 ttccttccag tctgcttatg aaaagaaatt aaaatatgtc gatcattggg aaggacgtta
    14701 ttttgcttat aggaaaggaa gtaatttcgg atttgctacc atgcagctac tcagtctgat
    14761 aggaaaagaa aaaggaaatg ttccgagttt ggcagaagaa gtgctcacag aattataaga
    14821 atcactagaa cttgtttgga ttatgaagag aaaattctgt gaaagctgtt gaaacgatga
    14881 caaaatcaac cactaggcag acataagcta tacttttatt tttccctatg atagaatact
    14941 tgcgacttag acttttcgaa tgggtgagag gatggaattg attgaaaaag cgccagccaa
    15001 gataaattta ggactggatg tattatatag acggcaagat gggtatcatg agttggaaat
    15061 ggtgatgtcg agcgtagact tggcagatca tctatttttt gaagaaataa cagaagacaa
    15121 aatcattgta gaaacaaata aagcattttt accggtagat gaacggaaca atgtttatca
    15181 agctgcttta ttgctaaaaa ataaatacaa tatcaaaacc ggtgtcaaga tctttgttaa
    15241 gaagaatatt ccagtagcag ctggattagg cggaggaagt acagactgtg cagctgctct
    15301 aagagggatc aaccaattat ggaatctggg ctgttcgtta gaagaactgg cagaaatcgg
    15361 tttggaagtc ggtacagatg ttccttactg tgtctacgga agtacggcat ttgtcggtgg
    15421 caaaggggaa gaaatccaag tattaccttc tatgccacag tgctgggttg tgttggtcaa
    15481 accaagaatg agtgtctcta cacgtacagt gtttagagaa gtagatacgc aagccatcca
    15541 acatcaagac atccacggac ttgctgaagc catccgagag caagattatc agcagatgat
    15601 caaaaaaatg ggaaacagtt tagaagatat aacgggaaag cggcatccag tcatccatca
    15661 aatcaaagag cgtatgatca agtacggagc agatgctgca ttgatgagtg gaagcggacc
    15721 aaccgttttt gccttgtgtc aacattattc aagagcgcag agagtatata atgggttaaa
    15781 aggtttttgt gaggaagttt atttagtacg aaccctgcaa taaaatataa gaagtaaaat
    15841 aggtgatgaa aaactgagga atcctaaaat ggtattcttc agttttttat tgtagtgaaa
    15901 tggctagata tgagcagcaa ctcatccatt ttacgattta atcaagaata tacatgctta
    15961 aatcagaaat tgatggattt ctattgactg tgttttttta ttttgataca ttagttcacg
    16021 ttaataaatc gtaaaattcc gatttgttca ttgaaaggag catataaatg aaaaaatatc
    16081 ttttgacagc tacggtaatg ataggtgctt tactgtttgc agcatgtgga aatacgaaca
    16141 aggaagcaga caaaaaagag gatttgacga tcgttacgac tttttatccg atgtatgatt
    16201 ttacaaaaga gatcgtaggg gatgaaggaa acgtgaaact tttgatccct gctggaacgg
    16261 agccgcatga tttcgaacca agtgcaaaag aaagagcaga aatttccgat gcagatgttt
    16321 tcgtgtataa tagttcagac atggaatttt ttgtggattc gttaaaggat tcagtggata
    16381 gcaaacagac cttgatgatc gaagcagcta aaggaatcga ccgtttagaa tcgcaagaag
    16441 cagacgaaca tgaagaatca gaagaagggc atggtcattc acatgaatac gatccacatg
    16501 tttggctcga tccggtatta gctataaaag aagtcagaac gattgctgaa gaattaggcg
    16561 aaaaatatcc agataaaaag gaaactttta cgaagaatgc cgatgcttat atcaaaaagc
    16621 ttgaagcact ggatcaaaaa tactccgatg agctaaaaga tgcaacaaac cgcacatttg
    16681 tcactcagca tgctgctttt gcttatttag cgaatcaata cgacttaaaa caagtagcga
    16741 tttcgggcgt ttcaccagat caggaaccca caccaagtcg cttagctgag ctaaaagaat
    16801 ttgtgaagaa aaacaacatc aaagtgattt attttgaaga aaatgcctct tcgaaggtag
    16861 ctgagacatt gtcgaatgaa acaggagtga agctagaagt gttgaatcct ttagaaagct
    16921 tgactaatga acaaatcaaa gcaggcgaaa attatatttc tgtcatggaa aaaaatctga
    16981 aagctttaaa agaaagtatc aattaagttt tagaatggag gttgtaaggt gcgttatatc
    17041 gaaatatctg atttgacctt ttactatgat gatgaacctg tactagaaga tgtctcttat
    17101 catgtagatg cgggtgaatt tgtcatttta actggagaaa atggagcagc caagtcaacc
    17161 ttggtgaaag catcgttagg attactgaaa ccttcaaaag gaatgataaa gattgctgag
    17221 aaaaatcaag aaggaaaaaa attaagtatc ggttatattc ctcaacaagt tgcatctttc
    17281 aatgcaggtt ttcctagtac ggtgatcgaa ttagtccgtt caggaagata tccaagaggc
    17341 cgctggttca agcgtctctc tgaaaaagat catcaacatg tacagcgtgc tttagaagct
    17401 gtagggatgt gggatatgcg aaatcggcga ataggtgaat tgtctggcgg acaaaagcag
    17461 cgaatcagtt tagcgcgtat ttttgctact gaccctgact tatttgttct tgatgaacca
    17521 acaacaggta tggatgaaga atccagaaat gaattttatc gtcttctccg acatagtgcg
    17581 catgtccatg ggaaatcagt tctaatgatc acccatgatc atgaagatat caaacagtat
    17641 gcggacagac agatccgact ggtgagaaag gaggattctc aatggcgttg ctttcatatg
    17701 aattcatgag aagagcgttt cttgccgcta tcttcattgc cggaatcgcc ccgatgctag
    17761 gtgtgttttt agtgatcaga agacaatcgc tgatggctga tacgctttct catgtctctt
    17821 tagcaggggt agcgttagga ttctttttga accttaatcc tacacttacg accatgctag
    17881 tcgttgtatt agcagctgtg atattggagt atctgcgaac gatctaccga acctattcag
    17941 aaatctccat tgccatttta atggctggtg gtttggcgct agcgttagta ttgatgaatt
    18001 tgagcggagg gaattcagca acaagtatcc aatcctatct gtttggttcg atcgtgacca
    18061 ttacatggga gcaagtcatc tttttaggaa tcttattcgt tgccatcggt atattgttct
    18121 ttttattcaa acggccgatg tatgttctga cttttgatga agataccgcc cacgtggatg
    18181 ggctacccgt tcatttgatg tcgatgatgt tcaatgtgat tactggtgtg gctattgctg
    18241 tgatgatccc gattgccggt gcacttttga tatccgcaat catggttctt cccgcagcaa
    18301 tcggtatgcg attaggcaaa agcttcaatg tcgtgattgc tatcagtgtt tgtgtaggat
    18361 ttctagggat gctgtcagga ctaactagct cgttttatct agatacacct ccaggagcaa
    18421 caatcacatt agtctttatt gctttgttcc tgattgtaaa tatcatcaaa cgaatattgg
    18481 ttgccttcag aagaaaaaat aactaaatta taaagaaaag aagccttata aaaacgaaaa
    18541 attttttata ggcttctttt tattttaata aataccgaac tattttctta ttttttcata
    18601 aaatgctcga atttttgaag aaaaacttat ataatgtaaa acgataaaat ttcttaaaag
    18661 gagttggaat aggtgaaagt acgtcgcagt gaacgtctag ttgacatgac acagtattta
    18721 ttgaatcacc cgcatgaatt gatttcctta acaagctttg ccgaacgtta tgaatctgct
    18781 aaatcttcta tcagtgaaga tttgggaatc atcaagaaaa catttaaaga acgagggtat
    18841 ggaaccttgg aaacaatccc gggtgctgca ggtggtgtat tgttcatccc tgaaatttca
    18901 tatgaagagg cgaaaaaata tattgaatct ctagcagaac gtctatcaga acaagaccgt
    18961 ctgcttccag gaggatatgt ttatctctct gatttgttag gagaacctga actgctgcgt
    19021 caagttggaa gaatcattgc ttctaaatac cttggtgaaa aaatcgatgc tgtaatgact
    19081 gtagccacaa aaggcgtacc tattgcccaa gctgtgtcct attatctgaa tgcgccattc
    19141 gttattgtac gccgggactc aaaaataaca gaaggatcaa cagtaagtgt caactatgtg
    19201 tctggatctt ctgaaagaat tgaaaagatg gaactgtcta agagaagttt gaaacgtggt
    19261 tcacgtgtcc ttgtagtgga tgatttcatg aaaggcggcg gaacagtcaa cggaatgaag
    19321 agcttgattg aagaattcga atcagaactt gttggcatca ctgtatttgc cgaatcaaaa
    19381 ttcaacggtc atcgcgccat cgatgattat acgtcactgc tttatgtcaa agatgtagat
    19441 actcaaacga aaaatattac ggttgtgcca ggaaattatt tttcagaata agaggttgtg
    19501 aaaaagccgc ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt
    19561 ttcataaatt tggcttaata ccgagaaact ggcttttgaa caccgtttat ctagaggttg
    19621 tgaaatcagc gtcctgtcct gagtattagc tcaataaacg gaaaaattgc tcctcatatt
    19681 ttgccgttta ttgagctaat accgaaggtc agctgattga aaaacgttta atgaagaggt
    19741 tgtaagccta ccgtttaact ctaagtcaca agaaacgatt tcaaaaaaca gcttttcatg
    19801 ttttatgagt agtgtgactt gtgaccgagg agttggcagg agagcaccgg ttaccaaata
    19861 gataggcccg ttatggtcta tctatttttt tatccagaaa acgctgggat aaaagaaaat
    19921 cacttgtttt gttgaaaaaa tgggtatact gggataaact gaaatcagca aactatattt
    19981 aggagtgacg cagttggacg cacgttatgc aattattttg gcggcaggga aaggtacccg
    20041 catgaagtca aaattatata aagttttaca tcccgtttct ggtaaaccta tggtagaaca
    20101 tatcattaat cgggtcagtg aaacgaaacc agatgaagtg attacgatcg ttggacacgg
    20161 agcagaacaa gttaaagcac aactaggaga acgcagtaaa tatgcgttac aagcagaaca
    20221 attgggaaca ggacatgctg ttttgcaagc tgcttctttc ttggaaggaa aaaaaggaac
    20281 aacgttagtt atcagtggcg atacaccatt attgactact gaaacgctga acaatctttt
    20341 tgaatatcac caaggaaaaa atgcaagtgc aacgatcttg acggcacaag cagaaaaccc
    20401 aacaggatat ggacgaatca tccgtgacca tattgggatc gtagaaaaaa tcgtggaaca
    20461 aaaagatgca acaccagaag aagctcttgt acaagaaatc aatacaggaa cttactgttt
    20521 tgacaacgaa gctttgttcg atgcgttaag caaagttgga acaaacaatg ctcaaggtga
    20581 atactatcta acagatatca tcgaaatctt aaaagaagaa ggccatacag ttgctgctta
    20641 tcaaacggat gattttgaag aatcaatggg agtcaatgat cggatcgcgt tagctaaagc
    20701 aaatgagatc atgagaaagc gaattaacca gatgcatatg gtcaatggcg taagctttgt
    20761 cgattcagca acgacataca tcgatgcagg cgtagaaatc ggtccagaca cattgatcga
    20821 agcaggtgta caaatccaag gaaatacagt cattggttct gattgtgtca tcgggtcaca
    20881 ttcaaaaatc gtagacagta ggattgagga tcacgtcgtc attgaaaact cagtcattga
    20941 aagcagccat gtcaagaaac atgcagatgt gggaccttat gcacatttac gaccaaaagc
    21001 tgaaattggc gaaaacgtcc atatcggaaa ctttgtggaa gtgaaaaatg ctcaaatcgg
    21061 caaaggaact aaagtcggtc acttgactta tgttggtgat gctacgctgg gagaagaaat
    21121 caatgtcgga tgtggtgttg tcttcgtcaa ctatgatgga aagaacaaac atcatacgac
    21181 tgtcggcgac cacagcttta ttggttctag cacgaatatc atcggacctg tcgaggtagc
    21241 taaaaacagt tcgattgctg ccgggtcaac aattacagac aatattccag aatatgcgct
    21301 ggcaattgcg agagcaagac aagtcaataa agaaggatat gcaaaaaaac tcccttatct
    21361 aaactaagac ttttgcaaaa tcacttgatt tatttaaaaa atatgactac tatttattag
    21421 gtagaattct agagttgtga aaaagaaagt ggaggtcccc atgtcaaaac attactttga
    21481 cccaagatta aaaatttttg cgttaaattc caatcgccca ttagcagaga aaattgcagc
    21541 agcagtcggc gtcgagttag gaaaatcatc tgttactcaa tttagtgatg gagaaattca
    21601 agtaaacatt gaagaaagta ttcgtggatc gcatgtctat gttattcaat caacgagcag
    21661 cccagtcaat gataacttga tggagttgct gatcatgatt gacgcactga aacgagcaag
    21721 tgcaaaaaca atcaacgtag tcatgcctta ttatggctat gcgcgtcaag accgtaaagc
    21781 acgtgcacgg gaaccaatca cagcgaaatt agtagcaaac atgattcaaa aagctggtgc
    21841 aacaagaatg ctgactttgg accttcatgc agtccaaatc caaggtttct ttgatattcc
    21901 agtcgatcat ttgatgggtg cgccacttat tgccaattat ttcttagaaa aaggaatcaa
    21961 aggcgatgat gttgtcgttg tttcaccaga ccatggtggt gtcacacggg cacgtaaatt
    22021 agcggaattt ttgaaatctc cgattgcgat catagacaaa cgccgtccaa aagcaaatgt
    22081 tgcagaagta atgaatatca ttggacaagt caaaggcaaa acttgtgtac tgatcgatga
    22141 tatgatcgat acagcaggta cgatcacatt ggctgccaat gctttgaaag aagctggtgc
    22201 aactagtgtg tatgcttcat gtacacatcc agtattatcc ggccctgctt tacaaagaat
    22261 agaagattca gctatcgagc gtctagttgt tacagactcg atctatcttc cagaagatcg
    22321 taaaatcgac aaaatcgatg aagtaagtgt tggcggacta atgggagatg caatcaaacg
    22381 tatccatgaa aacaaaccag ttagtccatt gtttgaaaca aaaaataaat aataaataaa
    22441 ctcacaaagg ggagtccaat tgggctgaga ttgaatctat ttctaaaccc ttcgtacctg
    22501 tatcggttat gcgagcgtag gaattgtgaa taaacagctg tttgactatt ttcgaaagct
    22561 gcttcctcct ttgtgacttt tttattcaaa ggaggaagtt tttttatggg aaaaaataga
    22621 gtttgggtag aaggaacgat tgttgcagcg ttagcgatgg tgttgtcgtt gattccgatc
    22681 cagatcggca gcagtttttc tatttcatta gggcagattc cattgactct ttttgcgtta
    22741 agaagaggga caaaggcagg gatgctggca ggtttcattt ggggcatcct acattttccg
    22801 ttgggacaag tttattattt aagtgtcgtt caagtgttga ttgagtaccc gattgcctat
    22861 acttttgcag gcgcagcagg gctcatggct tcaaatgtcc aaaaaggcta tgtagacgat
    22921 aatacgaaac aaattagaaa aagcatcgtg tttggtgcat tgattggcgc actttccaga
    22981 tatttttggc attttattgc aggagtggtt ttctggggag cgtatgcact atgggggatg
    23041 aatccttggc tcttttcttt cgtaatgaat ggtgccagcg ggttgagtac agctattgta
    23101 acgatcatcg ttttattagg gatccaacaa acgactcctt cgatttttgt gccagataaa
    23161 cgacaggagc tgtaaataaa aaagaagcaa gaaacttgag acagaatcat gtcctagttt
    23221 cttgtttcct ttttattttt tgataagaaa tagatctact tcaatggcag ataagaaaga
    23281 tcggcatctt ctaccaattg ccagtgtcga tcggtgacag agtagattaa agtagcgatg
    23341 atttcctctt ctcgataccg ggtgcctttt aatttccaaa tataatcttc aatcacatag
    23401 attgggatac gatatttacc atcgatcaaa ccaacttgat caaaagaaaa aatgatccct
    23461 tcctgcatca aattacgcat ttctttcaat aattccacac tgattttttc aacaggtgtt
    23521 tcttttttga aaaaagattt ttttacattg atcttatctg acaagatcga acttaattta
    23581 ctagcaaaaa tcctataaaa ttcatctaaa tagcgataat aaatccgatt cagctgatca
    23641 ttaagatcga agtatacaaa attattctgt agtttataaa aaaacggcga atgaagatgt
    23701 gttttcatat gaccaaaata taataattct gaaatttcta aaggtgtcaa ttctttcagc
    23761 atcgttaaat cagtaaagtc gatccacttc aattcatcga atccacgcct gttacgagaa
    23821 gaggaaaaga aacgctgaat attttctgta ccacggatta ctttcagccc tgtatgggat
    23881 tcatattctc cagcagaagc agatggatca agcaagagca agttttgagg aaaatgcacg
    23941 atactgtttg caaagtcagt agcagtcagc ccttttgtta aaacagcatt acttgtggta
    24001 tcaatatgta cataaactga ctcagtcatc atgttcctcc tcttatttcc tcttaatctc
    24061 ttttatttta caacatttgc attaaaaaag cttgtttctt ttattttaaa attccattac
    24121 aaaatcacgt tttcattgaa aacactctca ttttcaatta atttttgtga tttttgtatt
    24181 tttttattta aaggaggttt tatgaattgt tttcttatgg aatctacaaa gtcgaatacg
    24241 agactcatga cctttttatg acgtactggt atacttgaag aaaaaggaga gcgcgatgga
    24301 gataaaaaaa gtttactttt cacagaaaat gtcgctgtat tcgcctatcc agcaagcaaa
    24361 acaacccctt ccattttttc aaagtcatca agaaagtaga gacgcttttt ggcgttctgc
    24421 aaaaaagcaa tgtgctttta aagcagtatc gaaaaaatat accgtctatt ttactattgc
    24481 taaaagtaga ggagatgtta tttttaataa tctgttgatt gaaggtgaaa cttttgaatg
    24541 gaaaaaattt tttcggtata tggaagcttg cgctcgtttt tttattaaag aaaactgttg
    24601 ttttttattt caatctcctc tatcagaaga atggttgcgt atttttcata aaaacggcta
    24661 tcaaggaacc acacaattaa ataaaaagct ggtctatcat acagcacttg ttttaggtag
    24721 aggaggagcg cacggtgctt atcagattgg tgtgtggcag gcgctgaagg aacatgatat
    24781 caactttgag attatcacag gtacctcggt aggtgcgttg aacggcgcat tgattttaca
    24841 aggagatatg aaaaaagctg tgaatttatg gaaaaagctg tcgacaagac aagtgcttgc
    24901 gttaccagaa atggctgcag ttgaagacct tcgtgaacgc ttctatcgag aaagacgcca
    24961 aatgacgaaa acagcactaa ttgaaggcgg ggtttcttct gctccattgg aaaagatggt
    25021 caaagacaat ttggatttgt ccttactcaa acataatccc aaaatacgtt tatatacggt
    25081 gtctactaaa ctgccagaac tagcggaagt agtgactgat atccaacaga caaaaacaga
    25141 agaaatacca gattggatac tcgcctctgc ttcattttat cccgctatgg cttatcgaaa
    25201 gatcggaaaa aataaatatg cagacggtgg gtatcgcaac aaaataccta ttgatattgc
    25261 gattaacaaa ggagcaacag aggcatttgt cgtagatgtg caagggccag gcccagcgaa
    25321 aaaaattcgt atgccagaca tttttatcca ttggaaatgt caaacattgt ggacactagg
    25381 cagctttctg ctttttgata gccaaaggaa ccaattaaat ttacaattag gctatctgga
    25441 aatgaaaaaa cgattaggct attactatgg gaactggtat acatttcatt ctgttaaacc
    25501 tgcaagaaca tactggcgag gatttttgtc ttacttaaca aaagaaattc agttagaatt
    25561 atcttttttc aaaagcataa aattttggca aaaactgcgt aatttatata agaacagggt
    25621 cgtaccagag acatgcggat tggcgatgct agaactttta gcaaaaaaac attttttact
    25681 gcccaatgaa atctatcaag tagacatgat gattcaaatg atctgccaac aagatataaa
    25741 aagtatgccg gatgatttat tggcacaaat tggtcaatta agtactgaag aatggcgaag
    25801 gtatcgaaaa taccagcaaa aaatacaaaa tgagcgcacg aaaactgttt attttgatta
    25861 tctgctacaa ggaaaacgca aggatcgttt gcagcatgaa ttgcttaagc aaccagttga
    25921 cacattactg atcctatatt tatattatct caaggaggaa caaccatggc acaagaattt
    25981 tcatatgaaa tcgtagaaga aatcgctata ttgtcagaaa acaacaaggg gtggcgtaaa
    26041 gaactgaacc tagtcagctg gaatggtcgc ccgccaaaat tcgatcttcg agattgggcg
    26101 ccagaccatg aaaaaatggg gaaaggcctg acattgacga acgaagagtt tgaacaactg
    26161 caaaaagcaa tagaaaatat gtaatgagga aggaccctgt cgaatgaaaa gtatgactgg
    26221 ttttggcaaa gccatccgcg aaacaaaagc gtatcagtta gatattgaaa taaaaagtgt
    26281 caaccagcgt tttctagatg ttcaaatacg aagtcctaaa atcctgaact ttgttgaaaa
    26341 cgattttcgt cagcttatta aacagcagtt aagccgcggt cgaatcgaag tatttgtgaa
    26401 tctagcttat attggcaaaa acgaaaaaca gatcgtcatc aactgggaat tgattgattc
    26461 acttatcact caattgacag acggaattca acaacgatac ggtagtcaaa atcaagtgtc
    26521 aattagtcgg atggcggaac gcttcgtgct ggacgaaaac ttcgtaacgg tagaagaaaa
    26581 accggaagag agcatggaag aattggcaca gttagctcta gatacattga aaagcgcact
    26641 tgattctatt gaggcgagtc gagaaaaaga aggtcaagct ttagctttag tgttgaaaaa
    26701 gaatactgaa gaattcaaag aagtactaaa aaaactcgat agttttgtag aaatctatga
    26761 aaaggaattt caaataaggt atcaaaaaaa gctggaggaa tttcttggag cgaaagtcga
    26821 tgagcaacgg ttgttgacgg agctagccat cttgttggaa cgcggagata ttcacgagga
    26881 actggatcgt ctggcgatcc atgtacaaaa actagatgaa cttatcgaag cacaatgtcc
    26941 tgtaggtcgg gaattagatt tcctgatcca agaaatgaat cgggaaatca acacgatcgg
    27001 ttccaaatca aatgcaatcg aaattaaaaa tcaagtcgtc cagtctaaga cgatcttaga
    27061 aaaaatcaga gaacaagtac aaaatattga atgacgaaag aggtagagat aatgaaatat
    27121 tatgacgtaa cgttccacga attaagtgga aaaactgtgg ttaaacggga tatcccttct
    27181 gaaaaaaacg gctttgacgt atggaaagat gcctgtgcag actataatga gaacgaactg
    27241 tttattttga tcaacgacgg tgcttacgtg acgatgaatc ggaaatttat tgttcgaatt
    27301 gacacagaag aggtagaaga tccgacagaa aaagcacgtt ctcgaaaaga tgagatcatg
    27361 ggtgtagtga atactttgtc caatatgggc ttttagcaag gatgctgaac aaagaaatac
    27421 aataaataaa cagaattgga tttcttcgct tagcttcttt gctctaggaa tacatttaaa
    27481 ggttgtgaca aaattcgtgt cacaatcttt ttttattttg aataagtgat gttcaaaaac
    27541 cattttctcg gtattaagcc aaatttatgc aaaaatgtga gaaactattt tcacaaaatt
    27601 tttcttcata ctcgaaacta atcggcttct tcacgacctc ttatacacgg cgttcaaaag
    27661 cttgcatctg cgataataac cccaaccaaa cgaaaaacat gagaagctgt ttatgtttgg
    27721 ctgttcttat tacttgatgc aggacagctt ttttacgacc tcttggtaca gggtagcttt
    27781 tttaccaatt tctagctata attaaagata accttgaaag ttgcagaaaa aaagtgcaaa
    27841 ataaacgata agtacaaaaa gactagttga aaggacgtcg tcatgtcaga gcgtggatta
    27901 ttaatcgtgt tgtctggtcc atccggtgta ggaaaaggca cagtacgaaa agcaattttt
    27961 gagagtgagg ataatgattt tcagtattcg atctcaatga ctacccgtaa aatgcgtgaa
    28021 ggggaggtcg aaggcgttga ttatttcttc cgaagcaaag aagagtttga atcaatgatc
    28081 gaagctggag agatgctgga atacgcagaa tatgtcggga attattatgg aactcctttg
    28141 tcatatgtca acaagacatt ggatgaagga aaagatgttt tcttggagat tgaagtccaa
    28201 ggagcaaaac aagtcaaaga aaaagtgccc gatggtgtat tcatcttttt gactccgcca
    28261 gatttagcgg aattaaaatc acggatcgta ggccgaggaa cagatgcaga tgaagtaatc
    28321 gaaaaacgaa tgaaagttgc aaaagaagaa atcgaaatga tggctcttta cgactatgct
    28381 gttgtaaatg atgaagtgcc gctagcagtt gaacgaatca aaaacattat tgccagtgaa
    28441 cattttcgag tagatcgcgt aatcggtaaa tatattaaaa tgttaaagga gatgtagtct
    28501 tatgatgtta aaaccttcaa ttgactcact attagatcgt gtaaattcaa aatattctct
    28561 agtgatttta gcaagtaaac gcgcgcatga attggatgca agtgcacagc ctactctaga
    28621 ttcttttgat tcagtaaaaa gcgtcggtca agctttagaa gaaatcgatg caggaaatgt
    28681 agtcaatgat ccacatcctg aattaaaacg tgaacgtttg aagatggaag aagaggaacg
    28741 tcaagcacaa aaagagcgtg aacaacacga attggaatca cgtatccgtg aagagcaaaa
    28801 aatgtaataa agaaaaatag agcgaacatc taaaaagaaa agcaaaccca gctgtttcct
    28861 tttcaaacat tcgccactta ttgtctggta gaaaaggctg tgtctgaact ttttgacaca
    28921 gttctttttt ttcttatctg ctgttaattt tttgttagga tttggcagaa gataagacaa
    28981 atccatcgat ctctagtaaa ataggcaagg aactttttgt cgcttgataa gaaaagacaa
    29041 aaaaaggagg aaacgaacca tgacattaac agctgaagtc attgtggacg tacctactat
    29101 gcagaccgat cagccattca cttatttggt tccctctgaa atggaaacag ctttgcaagt
    29161 cgggatgcga gtagaagtcc cttttggtaa tggcaatcgt catgtccaag gatttgttat
    29221 ggctatccaa aaatcagaag aatcagctaa tccatctttg aaggcagtta ttcgtttgct
    29281 agaccttgct ccggtggtca atgaagaatt actttcatta gctgattata tgaaaaaaat
    29341 tacttacgca ttcaaaatca cttgtctaca aacgatgctt ccgagtgtga tgaaagcaga
    29401 atatgataaa ttgatctatc ctttggcaga cacaccagaa gttcaggtat tgtttgatca
    29461 gcgagaagtg atttcctgga aggaagcaga agaagaaggc agcctttctc aattgattcg
    29521 ctggcgccaa gagcaattag tagatatcaa atatgaagtc catacacgaa ataaagtaaa
    29581 aacgattcgt ttggtccgtt ctttgttaac tgaaaagcaa atcgaagaag aatgggcaaa
    29641 attacggcaa aatgccaaga aacaaaaaga acttcttttg tgtttatctg aactgagcca
    29701 agaagaacca attgcttatt tcaaaaataa aggaatcagt actgctgtct tgaatcaagg
    29761 aaaagaaaag ggctggctgg agtttataga atcagaacgt tatcgcgacc cttataaaga
    29821 ccgagtattt gaccaaacga cggcattgga actgaatgca gaacaaaaaa atgctgtcga
    29881 acaaatcatc acagcaggtc agcagcaaaa agatcaggtc tttttgctgg agggaatcac
    29941 tggcagcggg aaaacagaag tatatcttca ggcaatcgct gatgttttgt cagaaaataa
    30001 aacggctatt atgttagtgc ctgaaatcgc attgactccg caaatggtcg agcgattcaa
    30061 aggacgcttc ggagaatcag ttgcggtctt acatagcgga ctttcccaag gtgaaaaata
    30121 tgatgaatgg cgcaagattg agcgggaaga agcgcaagta gtagtaggcg cacgatctgc
    30181 tatttttgcc ccgctgaaaa atatcgggct gatcattatc gatgaagaac atgaatcaag
    30241 ttacaaacaa gatgagacac cgcgttatca tgcgagagat ctagcaattt ggcgcagtaa
    30301 atatcatcat tgcccaatag ttctaggaag cgcgacgccc tctttagaat caagagcacg
    30361 agctcaaaaa ggcgtttatc agttattgca gttaaaccac cgagccaaag cagctgcaca
    30421 gcttccagct attgagatcg tagatatgcg tgaagagttt cagaatcatc ggacctctac
    30481 tttttctgct aatttgcagg aaaaaataca aaatcgatta gacaaaaaag aacaaactgt
    30541 cctactgctc aatcgtcgag gatattcgtc ttttgtgatg tgtagggact gtggatttgt
    30601 tcttccatgt cctaattgcg atatctctct tacactgcat atggatacac gttcaatgcg
    30661 ctgccattat tgcggacatg aagaacctat tcccaatcgc tgtcctaatt gtggcggcaa
    30721 taaaatccgc tactatggaa caggtactca aaaagtagaa gaggaactcc gagaacttta
    30781 tccgcaagct aggattttgc gtatggatgt agataccact cgaagaaaag gggcacacga
    30841 acagatttta caaaaattcg gtgcaaaaga agcagatata ttattaggga cacaaatgat
    30901 tgctaaaggt ttggactttc cagaagtgac attagttggg gtgttgaatg ctgatacctc
    30961 cctgaattta ccggattttc gttctagtga gcatacattc caattgctta cccaagtttc
    31021 aggacgagca gggcgagcgg aaaaagcagg cgaagtcgtg atccaaacat tcaatcctca
    31081 acattacgcc atagaattgg ctaaaaaaca aaattatgag cagttttatc agcaggaaat
    31141 gcatgtacgg catcgtggag gctatcctcc ttattacttt actgtgaaaa taaccgccag
    31201 ccatcctgaa gaacaagtag cggcaaagaa aatctttcag attgccaatc agctaaaaga
    31261 agtgttaagc cctcaaagct tgctattagg gccaacgccc agcatgattt tgagagtgaa
    31321 aaaccgatat tattatcaac taatcatcaa atataaacat gaaccgaatc ttcctcatgt
    31381 tttggatgaa attttgaatg gaagccagaa agaacagcga caaggtcttt ttgtggcaat
    31441 tgacaacgaa ccattaaact ttatttaaga aagggccaaa ttatgcgcta tcctatattg
    31501 atccacccta atgataaatt aaaaagaact gctcagccaa tcgatgtaat cacggacgag
    31561 acaatcgctt tattagacaa tttatatgag acaatgattg cgaatgacgg aatagggatc
    31621 gctgctcctc aagtaggaca aaacaagaga attgctgtga tcgaagtaga tgaaggcgag
    31681 aaatttgaac tgattaaccc agaaatcatc gaagcaaaag gtgaaagcct tgacgtagaa
    31741 ggttgtctca gtattccgca tgtctatgga acggtcaagc gagcagatga agtgaccgtg
    31801 cgttattatg atcgagacgg agaagagata gaagtgacgg cttttggcta tttagcccgt
    31861 gccttccagc atgaaatcga tcacttagac ggtatcttgt tcattgagaa aatgatacag
    31921 cagatcccgg aagaagaact tgaagaatat atggaggaac atcttgatga ctaagctagt
    31981 atttatgggc acacctgctt tttctgtgcc gattcttgaa gggctgatag aatcaggtta
    32041 cgagatccag gcagtggtaa cacagcccga ccgtccagtt gggcggaaac gagtaattac
    32101 gccgacaccg gtaaaaaaag cggctctgaa gcacggtatc cgtgtgctgc agccagagaa
    32161 aatctccggt tcgccagaaa tggaagaaat cattgaattg gtgcctgatg tgatcgtcac
    32221 tgcggcattt ggccaattct tgcctgaaaa gttattgcaa gtaccaaaat taggggcaat
    32281 caatgtccat gcttctttac ttcctaaata tcggggaggt gcgcctgttc attatgccat
    32341 catgaatggg gagaaagaaa ctggtgtaac gatcatggaa atgatcaaaa aaatggatgc
    32401 aggcggaatc tatgcgcaag aaagcatacc aatcactaaa caagatgatg tagggacgat
    32461 gtttgaaaag cttagccttt taggaagaaa gctattgcta gaaacattgc ccaatatttt
    32521 ggatggacaa aaaccagtac ctcaagacga gtcagaagca actttttcgc caaacatcac
    32581 acgggaacaa gaagcaatcg attggcataa aactgcagaa gaaattgaca accaagtacg
    32641 cggaatgcgt ccttggccaa ttgcattcac gacctatgag ggcatacgct ggaaattatt
    32701 atcggttgaa gtattggcag aaactacaga caaagcacct ggtacgatca tccggaaaga
    32761 caaaaaaaat ctatggattg cttgcggtaa acagaccgtc ttagccatca atgaattaca
    32821 gccagctggt aaaggaaaac aggccgtaca tgatttcctt aacggaagcg gtcagcaagt
    32881 tcagattggt cagcaggtga aataatgaaa aaaatcccta aaaaaatgaa aaaatcagta
    32941 cgatatatgg cattactgac attagagcga gtgaacaagg gtggcgccta ctcgaatctt
    33001 ttgttgaatg agatgatcaa acaaagcgag ctaaatcaaa aagatgtggg tttatttaca
    33061 gaattgatct atggtacgat tagccatcag cgactattgg aattttacgt tgcaccattg
    33121 atcgctaaag caaaaaaagt ggatgactgg gtcaagatgc ttctttatct ttctgtatac
    33181 caattagaat ttttagataa agtccctgca catgctattt taaacgaggc ggtcgaaatc
    33241 gcaaaagtaa aaggaaatcc tgggacaggg aaatttgtaa atggtgtctt gaggaattat
    33301 cagcgtcaag gagcaccaga tctgcaaggc attgctgatc ctatcgaaag actttctgtc
    33361 aaaatcagcc tgcctctatg gatgacgaaa cgactggtgg atcaaatcgg atacgaagaa
    33421 actgaaaagc tcggtttatc gctatatcag cctagccatg ctagtgcccg agtagataca
    33481 cgccgacttt ccagagatca ggccatcact gttttgcaag aagaagagat cacagctgaa
    33541 aaaagccaag tcagtccata cggtatagta gcgaaaaaag ggcatttagc ttctagctca
    33601 ttgttccacg atggcgtcat gacgatccaa gatgaaagtt cgatgcttgt agcgccgtct
    33661 atgcaaatcg agcctgaaca tcatgtctta gatgcctgtg cagcacccgg aggtaagacg
    33721 acgcatattg cttctttttt agatccgtca tgcggtggag aagtggtcgc tttagatgta
    33781 cacgaacaca aagtcaaact cattgaagat aatgcagcac gtctaggtgt ctcttctgct
    33841 gtctacgctc aaaagatgga tgctagagaa gtcagtaaac agttcgaagc agagcagttt
    33901 gatcggattt tagtagacgc gccttgttca ggtcttgggc tgcttcggcg aaaaccagat
    33961 attcgttata aaaaacagcc ggatgaatta gcgaatttac caaaaatcca attggaaatt
    34021 ttagaaagct gcgcaccaac tttaaagtct tccggtattc tgacttatag cacttgtacg
    34081 atcttaaaag aagaaaacca agacgttgtt tctgctttct tggaaagaca ccctgatttt
    34141 gagaggattg atgttttgac caatcctatg ctacaatcat caatcgaaga gaaaatgttg
    34201 actttgtatc cgcatcagtt ttacacagat ggatttttca tttgttgttt gcgcaaaaag
    34261 taatgaggtg aaaaaatgca gattgaatat caatcagatg taggaagacg acgtaacaca
    34321 aatcaagact atgccagtgt atttaccagc cgttcaggtc tcaagcttgc tgtattagca
    34381 gacgggatgg gtggtcatcg tgccggagac attgccagtc agatggcggt aacgaatcta
    34441 ggcgaagctt gggaaaaaca agaagctgtt gacgatgaaa aaatcgccca atggttcatt
    34501 caatcgattc aagaagaaaa tatgctgatt tatcagcgtg gacaagaaca gccggaatat
    34561 aacggtatgg gaacaacaat cgtagcggcc gctctgtcag atgactggtt cacgattgcc
    34621 aatgttgggg atagcagagc ttatttgatt cgtgaagata aaatcatgca attgacggag
    34681 gatcattctt tagtcaatga actagtcaaa tcaggagaaa tcagcgaaga aatggcagtg
    34741 aaccatccaa ggaaaaatat tttaacacgt tctgtcggca tgcctggaac agtcgaagta
    34801 gatgtttcta ctcatctttg gcaactaaaa gatcgactgc tgttatgttc agatggcttg
    34861 acgaacatgc tgtctgaaga agaaatagga acgatcatca atcaagaagg cccattgtct
    34921 cataaagtaa ctgagctgat tgatcgtgcc aatgaagcag gcggagcgga taatatcacc
    34981 gttcttctta tcgaatataa ggaggacagt gtatgatcga gttgggcaaa aaattaaatg
    35041 gccgttatca aatcactgga aatatcggta gcggtggtat ggcaaatgta tttttggcgc
    35101 acgatttaat cttggatcgt gatgtagcag ttaaagtctt acgatttgac tttcaaaatg
    35161 accagactgc gattcgacga tttcagagag aagctttagc agcaacagaa ctcgttcatc
    35221 caaacattgt tagtgtttac gatgttggtg aagaagacaa catgcagtat ctggtgatgg
    35281 aatacgtcaa aggaatggac ttgaaacgat atatccaaac acattatcct gttccttatg
    35341 aaacagcagt aaatattatg cagcagatcc tttctgctat ttctttagct cataaccatc
    35401 aaatcatcca ccgagatttg aaaccccaaa acgtactgat cgataatgaa ggtgttgtaa
    35461 aaatcacgga tttcgggatt gctatcgctt tatctgaaac atcgatcacg cagacgaata
    35521 cgatgctagg atctgttcat tatctgtccc ctgagcaggc cagaggaagt atggcaacga
    35581 agcagtcaga tatttatgct ttagggatca tcctttatga gatgttgacg ggtagcgtac
    35641 cgtttgatgg tgaatctgct gtaacaattg ctttaaaaca ttttcaagat gatcttccat
    35701 ctataaaagc acttgatcca aatgtgccac aagcactgga aaacgtgatt ttacgggcaa
    35761 cggctaaaga acctgcagac cgttataagt cagccgagga aatgtctgat gatctttcta
    35821 cagtattatc accagctcgt gcaaacgaag aaaaatggca gcctcatgtg atggataatg
    35881 aaacaaaagt tatcacaccc cttacggaag atgcaccaat gcctgattca ttcaaatcta
    35941 tgcctctgcc aaaagacaag ccagaaaacg aaaccttacc agaggaggaa gaagttctgc
    36001 ctgatcctaa aaagaaagga cagaaaaaat ggtggctgat cctattagct attcttgctg
    36061 ttttaggaat cggaacagct gtattctttg cttctggcgg gcgaggagaa gttgttgttc
    36121 ctgatgtttc tgatctttct gaagcttctg ctagagaaac actcgcaaaa gcgaaattag
    36181 aagtagcaac aaaaacagaa gaaattgcag atgacaaaat cgaagaaggt aatgtagtga
    36241 agacagaccc ggcagcggga acgacagtca agcaaaaacg cgaagtcact ctgtatatta
    36301 gtagcggcac gaaaaaaatc aaattagatg attacagtgg taaaagttat gaagatgccg
    36361 aagcaagttt gaaagaatta gggttttctg ctgacttgat tcaaaaagaa gaggaattca
    36421 gttcagaggt agaaagcgga atgatcattt cgcaatctcc atcagaagat acagaagtcg
    36481 atcctaaaac agacacgatt actttcacag tcagcaaagg accagaaaca gtgactttga
    36541 gtagtcatgt aggagaaaac tacgaaactg ttcgtaattg gcttgttaca caaggattca
    36601 atatcaataa tattagtgct acttatgact atagcgacac tgtaccatca ggttcgatca
    36661 tcagtcagga tccagcctcg ggtgaagttg tagcagaaga aacttacatc aattttgtcg
    36721 taagccaagg aacagagcct aaattgaatg atatctccgg ctatacaaaa agcgaagcac
    36781 aaagttattt agctagtgtc ggtgcagaat atatcgggca tgagacttcc gaatattcaa
    36841 acacggtgga taaagataaa gtgattcgta cagtaccagg ggcaggaacg acaattacca
    36901 aaggaatggt tgtgaacgta atttactcta aaggaccaga tccagcgtct tctagttctg
    36961 aaaaagactc ttcagacagt tctagcagct caagtagctc cagtagcaca agcaactctt
    37021 caaaagaaga atcttccagc tccacaagtt catcagctag cacctcagaa agttcaaaat
    37081 aagtgctgaa taaaatatat cagaagggaa tgcgacattg atttgttgca ttctcttttt
    37141 tgtgtttcta tctcttttga tcaattttca agccatatac tttgagaagt ttgcatggaa
    37201 taacacctac tttcctcttc gtatggtaaa ataaacaaaa acggaggtgg cacttatcaa
    37261 aggacaaatc agaaaagcaa tcagcggttt ttactatgta tacgcaaacg gagagaccta
    37321 tcaaacaaga ggaagaggaa atttccggaa tcggaaaatc acgccgcttg tcggagatca
    37381 ggtgatcttt gagagtgaaa atcaaacaga cggctatctt ctggaaatct tgccgagaaa
    37441 aaatcaatta gttcgcccac cagtcacaaa tgttgatctt ggaatcattg tcacaagtat
    37501 cgtagagcct gcattttctt ataatctact tgaccgcttt ctagttacct tggaatacga
    37561 gtcgatcgaa ccgattattt ttttgacaaa gaccgacttg tcagacgacc cagaaaaaat
    37621 cgaagagatc aagcaaacct atactaaaat aggctatccg gtgattgtac ccaatcatac
    37681 gggtgatacg acagaactga tccgttactt cccagaacgg ctatcggtct ttatgggtca
    37741 gtctggagca ggaaaatcga cgctactgaa tcaaatatca ccggaactga atttagcaac
    37801 aggagagatt tctgatgcac ttggaagagg acggcataca acacgtcatg tggaactgat
    37861 tcctatctat ggcgggttag tagcagatac accaggattt agctcgattg actttttgac
    37921 aattgaagca ggagaactgc cgagacaatt tcctgaattt gtagaagctt ctgtccattg
    37981 caaatttaga gagtgcatgc atgctaaaga acctggctgc gaagtaaaac atagagtaga
    38041 aacaggtggt atcgcacaga cacgttacga caattatttg caattcttac aagaaatcga
    38101 aaaaagaaaa ccaatgtatg caaaaaaaga aggtaaacga aaataggagg aaacaagaat
    38161 gaagatagca ccatccattt tgagtgcaga ttttgccaat ttacaaagag acattgagtt
    38221 agtggaaaaa ggaggcgcag attacatcca cgtagatgta atggatggcc gatttgtccc
    38281 aaatatcaca ttagggccaa atattgttca ggcaatccgt cctgttacta aattaccgtt
    38341 agacgtccat ctgatgatcg ttgatccaga aaactacatt ccagcatttg caaaagctgg
    38401 agcagatatc attactgtcc acgtcgaagc aactcctcat attcatcggg cacttcaaat
    38461 gacaaaaagc ttaggagtta aagctggagt agttatcaat ccaggaacac cagtctccat
    38521 gatcaaacat gttttgcctt tggctgatca agtactagtc atgactgtca atcctgggtt
    38581 cggcggacaa agctttattg aagaaacagt agaaaaaatt acggaactcg ctgatctacg
    38641 tagagagaac aattggactt atgcaatcga agtagatggc ggaatcgttc cagaaacagc
    38701 aaaaatctgt aaagatgcag gggcggatgt cttcgttgca ggatcatata tctatggagc
    38761 cgaaaatcca gtcggacaaa tcgaacgatt aaaagaggca ttagactaaa tgaaaatttt
    38821 attagctgct ggaggtaatc cgcaaaactg gccgcagtct ctgctgtcca aatacgatgc
    38881 ctatgtcggg atagaccgtg gtgcgctgta cctgcttcaa gcaggctacc cagtagatct
    38941 ggcagtgggt gattttgatt cattgtcgaa cgaagagcag aaggaagtat ttgcaaaagc
    39001 caaagagtat acccgttccc ctgcagaaaa agacgataca gatacacagt taggtatctt
    39061 aaaaacattt gaacgttacc cgcaagcgga agtcactttg attggagcta cgggcggaag
    39121 attggatcat cttttagcca atctctggtt aggtatggaa cctcgcttca aaccctttgc
    39181 ccatcagctt attatcagag attgtcaaaa ttatttgtct tattatttgc ctggagagca
    39241 tatagtaaga cagataccag atatgaagta tttagcttat tgctgcttga ctcccgtctc
    39301 gaatcttaca ttaaaaggca gcaagtatgt attggaaaat aaagaagtag aatatccgac
    39361 atcttatgca agtaacgaat ttctttcaga taccgcagaa ttttcttttt cagatggtat
    39421 gattgcagtt attcaaagca aagattgatc cataaacaaa taaaaaaatt tgtctttcag
    39481 gctaaaaatg acagaatcta ttatttgcta aaaaaaagct taagaggagc aacttacctt
    39541 ttgtttcacg gcatttagtg ataaaattga acacaagagt ctaattaatt cgaggtgtct
    39601 ttgtgaagaa gttcaatcct aataaaaata ttattattac cttggttatt gttatcatcg
    39661 tggtgacgat actaagtgtg accgctgcgc agcgagcgaa tgaaggaaag cccaatttct
    39721 tccaatccat cgttaatgac agtgttgcaa tggttgataa aacaatctct gccccagtac
    39781 ggtgggtacg aaatggagta gattctgtcc ataatctttt ttcaacttat gccgaaaatg
    39841 agcggctaaa agaaaaaatc gatagctatg atgacgtttc acagcaaaac aagaattacg
    39901 agagagaaat cgaagcgttg aaaaaagaac ttgagttgaa tcagacgctg actagttacg
    39961 aaaaagtaac ggcaaatgtc attactcgtt cgcctgacag ttggcaggac atgctgatcg
    40021 tagacaaggg ttctaaagac ggtatcgaag caaatatggc agtcatgtcg caaaaaggat
    40081 tgattggccg agtgatcgag gtcaatacgg cttcgtctaa aatcgaatta ctgtcatcct
    40141 ctaatgaaag ctccaatcat tttccagtac gggtatcttc ggctaatggc gaagcgtttg
    40201 gtttgcttaa aaactatgat gaaaagctcc atgccttagt ggtgacccaa ttaactggtg
    40261 atacggatat caaagaaggg gatgttgtcc agacatccgg tcttggaggg aattctccag
    40321 ctaacttgcc gatcggtacg gttattaaaa cgaaaccaga tagttatggg ctggatcggg
    40381 aagtttatgt gaaaccttat gcagaaatgt atgacgtgtc agttgtgacg attgtccaac
    40441 ggctagcgga ggattagtcc atgttgaaga aagaaacaat gaagtactat ctgccaatcg
    40501 ttttgttctt tttgatgttg atagatggtc atttaacaag aatgctaggg gagtggtcga
    40561 aaggcaccta tatgtcaaat gcccactttc tgatattggc attattatgt tgcagtatgg
    40621 cgtttgaaaa acgttattta ctgattacca cgattgttct cggggctatc tatgacgctt
    40681 actatattgg cgttatcggt atctatgcag tagctctccc tttaattgta tggttgatgt
    40741 atgtaatgaa agacgttatc catgtcaaca tctttactga atttttcagt atgatcatct
    40801 ttgtcacggg ttatgaattg tttacgatgg tggtccagtt gatttttaaa ttagcagtag
    40861 taaataacac gtattttatt acaaggtttt taggacctac actgctgttg aacatgatta
    40921 tatttgtatt attcattttt ccctttaaga aattattcag tgatgaataa tttctttttt
    40981 tatttttagg tcgaaagcga aaaagaatcg ttgttatctt aagaaatgct agacttaacg
    41041 cggctttttc atgatcattt tctgaaacga acagttttgt catttttaaa agttgttttg
    41101 aaacattttt gtaacataac tattatatcg ttgacacact gctatggtac aatttttata
    41161 tcgttaagaa agacttaaat atatttagat atttattaaa ttaagccagt cggaggaatg
    41221 aagagtgaaa aagagtttaa tatcagcagt aatggtatgt tcaatgacct tgactgccgt
    41281 agcatcaccg atcgctgccg cggcggacga ttttgattct cagatacaac agcaagatca
    41341 aaagattgca gacttgaaaa atcaacaagc agatgcacaa tcacaaatcg atgccttaga
    41401 aagccaagtt tctgaaatca acacacaagc acaagaccta ttagcaaaac aagatacatt
    41461 gcgtcaagaa tctgcacaat tagtaaaaga cattgcagat ttacaagaac gtattgaaaa
    41521 acgtgaagat acaatccaaa aacaagcacg cgaagcacaa gtaagcaaca caagctcaaa
    41581 ctacatcgat gctgtattga atgctgattc attagcagat gctattggac gcgttcaagc
    41641 aatgacaacg atggtaaaag caaacaacga cttgatggaa caacaaaaac aagataaaaa
    41701 agctgtagaa gacaaaaaag ctgaaaacga tgctaaacta aaagaattag ctgaaaacca
    41761 agctgctttg gaatctcaaa aaggcgatct tttatctaaa caagctgatt tgaacgtact
    41821 gaaaacttca ttagctgctg aacaagcaac tgctgaagac aagaaagctg acttgaaccg
    41881 tcaaaaagca gaagctgaag cagagcaagc tcgtattcgt gaacaacaac gtctagcaga
    41941 acaagctcgt caacaagctg cacaagaaaa agctgaaaaa gaagcacgcg aacaagcaga
    42001 ggctgaagca caagctactc aagcttcatc tactgcacaa agctcagcaa cagaagaatc
    42061 atcagctaca caatcttcaa tgactgaaga atcatcatca gctacacaat cttcagcaac
    42121 agaagaatca acaacacctg aatcttcaac agaagaatca acagcgcctg aatcttcagc
    42181 aacagaagaa tcaacaacag cgcctgaatc ttcagcaaca gaagaatcaa caacagtgcc
    42241 tgaatcttca gcaacagaag aatcaacaac agtgcctgaa tcttcaacaa cagaagaatc
    42301 aacaacacct gcgcctacaa caccatcaac agatcaaagt gttgatacag gaaacggcac
    42361 aggaagtagt actccggctc caacgccaac accaacacct gaacaaccaa aacctgtaac
    42421 acctgctcca gctccttctg gaagtgtgaa tggtgcagca atcgtagcag aagcttacaa
    42481 atatattggt actccatatg tttggggcgg taaagatcca agtggctttg actgctcagg
    42541 gttcacacgc tatgtttaca tgcaagtaac tggtcgtgac atcggtggtt ggacagttcc
    42601 tcaagaatca gctggaacaa aaatttcagt ttctcaagca aaagctggag acttgttatt
    42661 ctgggggtca caaggcggaa cttaccacgt agcgattgct ttaggcggag gacaatatat
    42721 ccatgctcct caaccaggcg aaagcgtaaa agttggttca gttcaatggt ttgcccctga
    42781 ctttgctgtc agcatgtaag aatga
//
LOCUS       DAZAFF010000011        42784 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000011 DAZAFF010000000
VERSION     DAZAFF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.011"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1252)
                     /locus_tag="ACG9BB_000612"
     CDS             complement(44..1252)
                     /locus_tag="ACG9BB_000612"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFN2836584.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(1364..1696)
                     /locus_tag="ACG9BB_000613"
     CDS             complement(1364..1696)
                     /locus_tag="ACG9BB_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFN2836585.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1844..2719
                     /locus_tag="ACG9BB_000614"
     CDS             1844..2719
                     /locus_tag="ACG9BB_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN2836586.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(2801..3382)
                     /locus_tag="ACG9BB_000615"
     CDS             complement(2801..3382)
                     /locus_tag="ACG9BB_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFN2836587.1"
                     /translation="MLIDVREKKIFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(3388..4647)
                     /locus_tag="ACG9BB_000616"
     CDS             complement(3388..4647)
                     /locus_tag="ACG9BB_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN2836588.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(4649..5695)
                     /locus_tag="ACG9BB_000617"
     CDS             complement(4649..5695)
                     /locus_tag="ACG9BB_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836589.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(5930..6205)
                     /locus_tag="ACG9BB_000618"
     CDS             complement(5930..6205)
                     /locus_tag="ACG9BB_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFN2836590.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(6448..7758)
                     /gene="der"
                     /locus_tag="ACG9BB_000619"
     CDS             complement(6448..7758)
                     /gene="der"
                     /locus_tag="ACG9BB_000619"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFN2836591.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(7940..9169)
                     /gene="rpsA"
                     /locus_tag="ACG9BB_000620"
     CDS             complement(7940..9169)
                     /gene="rpsA"
                     /locus_tag="ACG9BB_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFN2836592.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9304..9984)
                     /gene="cmk"
                     /locus_tag="ACG9BB_000621"
     CDS             complement(9304..9984)
                     /gene="cmk"
                     /locus_tag="ACG9BB_000621"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFN2836593.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10033..10668)
                     /locus_tag="ACG9BB_000622"
     CDS             complement(10033..10668)
                     /locus_tag="ACG9BB_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFN2836594.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(10734..12137)
                     /locus_tag="ACG9BB_000623"
     CDS             complement(10734..12137)
                     /locus_tag="ACG9BB_000623"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFN2836595.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(12124..13155)
                     /locus_tag="ACG9BB_000624"
     CDS             complement(12124..13155)
                     /locus_tag="ACG9BB_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2836596.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13186..13407
                     /locus_tag="ACG9BB_000625"
     CDS             13186..13407
                     /locus_tag="ACG9BB_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFN2836597.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(13572..14309)
                     /locus_tag="ACG9BB_000626"
     CDS             complement(13572..14309)
                     /locus_tag="ACG9BB_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFN2836598.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(14462..15802)
                     /locus_tag="ACG9BB_000627"
     CDS             complement(14462..15802)
                     /locus_tag="ACG9BB_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN2836599.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16039..17562)
                     /locus_tag="ACG9BB_000628"
     CDS             complement(16039..17562)
                     /locus_tag="ACG9BB_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFN2836600.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(17940..18551)
                     /locus_tag="ACG9BB_000629"
     CDS             complement(17940..18551)
                     /locus_tag="ACG9BB_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN2836601.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(18674..18799)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(18881..19597)
                     /locus_tag="ACG9BB_000630"
     CDS             complement(18881..19597)
                     /locus_tag="ACG9BB_000630"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFN2836602.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(19603..20172)
                     /gene="scpB"
                     /locus_tag="ACG9BB_000631"
     CDS             complement(19603..20172)
                     /gene="scpB"
                     /locus_tag="ACG9BB_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFN2836603.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20156..20926)
                     /locus_tag="ACG9BB_000632"
     CDS             complement(20156..20926)
                     /locus_tag="ACG9BB_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFN2836604.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(20923..21294)
                     /locus_tag="ACG9BB_000633"
     CDS             complement(20923..21294)
                     /locus_tag="ACG9BB_000633"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2836605.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21313..22200)
                     /gene="xerD"
                     /locus_tag="ACG9BB_000634"
     CDS             complement(21313..22200)
                     /gene="xerD"
                     /locus_tag="ACG9BB_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFN2836606.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(22296..22769)
                     /locus_tag="ACG9BB_000635"
     CDS             complement(22296..22769)
                     /locus_tag="ACG9BB_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN2836607.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(22874..23737)
                     /locus_tag="ACG9BB_000636"
     CDS             complement(22874..23737)
                     /locus_tag="ACG9BB_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFN2836608.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(23933..26032)
                     /locus_tag="ACG9BB_000637"
     CDS             complement(23933..26032)
                     /locus_tag="ACG9BB_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFN2836609.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(26252..27361)
                     /gene="rpoD"
                     /locus_tag="ACG9BB_000638"
     CDS             complement(26252..27361)
                     /gene="rpoD"
                     /locus_tag="ACG9BB_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFN2836610.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(27385..29226)
                     /gene="dnaG"
                     /locus_tag="ACG9BB_000639"
     CDS             complement(27385..29226)
                     /gene="dnaG"
                     /locus_tag="ACG9BB_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFN2836611.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(29408..31723)
                     /locus_tag="ACG9BB_000640"
     CDS             complement(29408..31723)
                     /locus_tag="ACG9BB_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN2836612.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(31908..32768)
                     /locus_tag="ACG9BB_000641"
     CDS             complement(31908..32768)
                     /locus_tag="ACG9BB_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFN2836613.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(32827..33459)
                     /locus_tag="ACG9BB_000642"
                     /pseudo
     CDS             complement(32827..33459)
                     /locus_tag="ACG9BB_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            33626..34387
                     /locus_tag="ACG9BB_000643"
     CDS             33626..34387
                     /locus_tag="ACG9BB_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN2836614.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            34413..37004
                     /gene="mprF"
                     /locus_tag="ACG9BB_000644"
     CDS             34413..37004
                     /gene="mprF"
                     /locus_tag="ACG9BB_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFN2836615.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(37192..38379)
                     /gene="tuf"
                     /locus_tag="ACG9BB_000645"
     CDS             complement(37192..38379)
                     /gene="tuf"
                     /locus_tag="ACG9BB_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFN2836616.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            38747..39103
                     /locus_tag="ACG9BB_000646"
     CDS             38747..39103
                     /locus_tag="ACG9BB_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN2836617.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(39344..40348)
                     /locus_tag="ACG9BB_000647"
     CDS             complement(39344..40348)
                     /locus_tag="ACG9BB_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFN2836618.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            40514..41365
                     /locus_tag="ACG9BB_000648"
     CDS             40514..41365
                     /locus_tag="ACG9BB_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN2836619.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            41382..41627
                     /locus_tag="ACG9BB_000649"
     CDS             41382..41627
                     /locus_tag="ACG9BB_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836620.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            41814..42518
                     /locus_tag="ACG9BB_000650"
     CDS             41814..42518
                     /locus_tag="ACG9BB_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836621.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT        12069 a         8969 c         7363 g        14383 t
ORIGIN      
        1 tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc aatttttctt
       61 tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct acgacggctt
      121 tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca gtttcttcta
      181 gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa cgatcatagg
      241 attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat tcttctactt
      301 gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct ccagtttctc
      361 gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta gaacacttcc
      421 acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg atcaataatg
      481 cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa cgtaaaagtg
      541 cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca ataaatgcat
      601 ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca tttatacgtt
      661 ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag gtttcttcct
      721 ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt aaggcatctt
      781 catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct agatcttgca
      841 gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca ttgatcgtaa
      901 aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga tctggtctac
      961 ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt tcatcatttt
     1021 ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga aaaagctgtt
     1081 tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc tggttcagca
     1141 agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc tctaattttt
     1201 tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc atttttccaa
     1261 cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga ctgtttgaca
     1321 agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg tccttacgct
     1381 caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa acttcagtta
     1441 agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca ccaagttctt
     1501 ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa gtgtggttga
     1561 cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct aatggagaga
     1621 aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata ctgtataaag
     1681 aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt aacgcaaatc
     1741 ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag gaatttctaa
     1801 atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca aacgaaaaac
     1861 agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg gtcttgttta
     1921 cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct tgatcttaag
     1981 agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc cattgatttt
     2041 actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag gtacactttc
     2101 tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt tacagcatga
     2161 tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg ggatcgtcta
     2221 ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag aacaaaatta
     2281 cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac ttgcttcatt
     2341 aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg ttttcagtcg
     2401 tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg tcgtctccaa
     2461 caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg tgactttcct
     2521 ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg tcgttagctc
     2581 tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag ctttcctttc
     2641 aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc cacagacaag
     2701 attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag attccacatc
     2761 cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta tgttcactta
     2821 cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca tcagataatt
     2881 tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca taaagcattt
     2941 ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca gtatcttctt
     3001 cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat agatattctc
     3061 ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc caattcaaac
     3121 ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa aacaatttca
     3181 tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca aaattcgccc
     3241 cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa ttcaagatcc
     3301 aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa aaaatcttct
     3361 tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta ataatgacag
     3421 tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt gcgctctttt
     3481 cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg gttcatgtga
     3541 tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt ctaattcatt
     3601 ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca tctgattgat
     3661 cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta aagtaagtaa
     3721 tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt ctgctcgctg
     3781 tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga cttggtaagg
     3841 attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt cttcttcttg
     3901 aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt ctcgaagttc
     3961 ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa aagctagctg
     4021 gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta cgatagctgc
     4081 ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg agattcctga
     4141 aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct ctttgaaacg
     4201 atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat caggaagcaa
     4261 cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa cttggtacaa
     4321 atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca ctttttccag
     4381 gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta acgggatgtt
     4441 cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt ttgcttcgat
     4501 caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg ctgggtcttc
     4561 ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg ataaagcttg
     4621 caacattttt tcgctatttg tattcatttc aattccctct tatctcactg acgatttgtt
     4681 gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat ccgtctatta
     4741 tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa agaacttttt
     4801 ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca agcaagtcca
     4861 gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga ttccacacga
     4921 aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg ccattttcgg
     4981 tcagataaaa agcttgttca aacagtttac cataatttga caggttttct gcctctaatt
     5041 gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt aattgctttt
     5101 tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc attccgcaac
     5161 agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg attgtttgat
     5221 cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt ggtagctgca
     5281 aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca gggatcatcc
     5341 atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc atgatccctt
     5401 catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg acgaaatcaa
     5461 gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata taccagtcat
     5521 tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca taatagcgct
     5581 gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga ctgactagcg
     5641 tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt tccatacgca
     5701 tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa agataagagc
     5761 aaagctcacc tcataacttg caaataaata aaaattaaat aaaatacatg tgttttctat
     5821 gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc taggaaataa
     5881 aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct tatttaacag
     5941 cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga atttcgattt
     6001 cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact tcaaagttac
     6061 caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata gttgaaaata
     6121 cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa gcaacttttt
     6181 cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga gatatgatag
     6241 aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa atattgatta
     6301 acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg tttttaccaa
     6361 ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt tttcttttga
     6421 aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa ttcggatgga
     6481 cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt cataagaaaa
     6541 atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga cagcgacttg
     6601 tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat tgattgctac
     6661 agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct ggttcatact
     6721 tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg cagaaacaaa
     6781 aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt cgaaatcacg
     6841 cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa taattccacg
     6901 accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc cttcttcagc
     6961 attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac gcatcacgct
     7021 gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg tatctatcat
     7081 taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc gggtcgttcc
     7141 ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca aagaggattt
     7201 accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct cttcttcagt
     7261 ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta gtccatggct
     7321 tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga tatcgtttct
     7381 catttctgga ttatctactt tattgactgc taaaatcact ggtttattac ttctgtataa
     7441 aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga ctacacaaat
     7501 gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat ccataaaagg
     7561 ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac ctaaccattc
     7621 accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa tcgagatacg
     7681 ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc caacgattgc
     7741 aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac ttagtttaac
     7801 gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt ttattcctta
     7861 ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc tttcatctca
     7921 gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct tctacttctt
     7981 ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct tctggtaatt
     8041 cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct aaagctttga
     8101 tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact ttcacttcgt
     8161 caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt tgagagatat
     8221 ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt gtcaaacgtt
     8281 taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct gaccaaggtc
     8341 caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg atagacaaca
     8401 ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct acatgactat
     8461 gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca aatgcgccaa
     8521 aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata gttgaaagaa
     8581 tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat aagatcaaac
     8641 gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct ttatagtctg
     8701 aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca cgtacaccga
     8761 cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg accttgccct
     8821 cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg cgttttgaaa
     8881 gtagatagct gccgttttct ttatctttgc cgatcgtact gataacgact aaatctaata
     8941 catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat aattcttttg
     9001 ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt ttgtcttcaa
     9061 tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg cttttcattg
     9121 cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata acacttgtcc
     9181 tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga ttattcaaaa
     9241 aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat tttttttctt
     9301 aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg caacgacttc
     9361 tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct tcagcggaga
     9421 gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca gtgtttcaag
     9481 atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac gctcacttac
     9541 gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc cgatgtctct
     9601 tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa ctaattcttc
     9661 tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg gttgacggat
     9721 ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt gttgcgaaaa
     9781 agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa aagatacatt
     9841 gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg tataaatata
     9901 attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag caggcccgtc
     9961 gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa aagccgcaaa
    10021 gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa agttgtttgg
    10081 atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct cagccacttg
    10141 ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg atgaagaagg
    10201 tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct cacttgagga
    10261 agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat cttgagacac
    10321 agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg actcctcttt
    10381 tgttgtactt gtagaacttt ctactactga tgtcgaagtt tcagaagaag ttgctgctgt
    10441 tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa tagcgataca
    10501 taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct tttgttgtct
    10561 ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt cccatggttc
    10621 ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg ttttacctcc
    10681 taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa aaatcaatgt
    10741 tccatgtttt ttttaaataa atttagtaaa atcgttcccc aatgaagttg ttcagtctta
    10801 gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc acaacaattc
    10861 tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct gcggcagttt
    10921 tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt ttcctgacgt
    10981 ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc catccatttt
    11041 ttttgcagct catcaaaggg agcttgctct gtggcatatt ccaaataaag ctcaaaactt
    11101 tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc cccactttga
    11161 taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat ttcttgtaca
    11221 tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc agatttatcg
    11281 atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt tacaaactgc
    11341 tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc aactgtaaaa
    11401 cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca ataaatgatt
    11461 gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg ctgcgttttc
    11521 cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt tggtggtcga
    11581 tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc cgttagtgcc
    11641 agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata ttctggacgg
    11701 aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa cgcaatattt
    11761 tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact caaaaacaaa
    11821 aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga aagtaaacta
    11881 ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg atcttccatt
    11941 agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc cgtcaattga
    12001 tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc tccatcaagc
    12061 aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa atattttttt
    12121 aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt gatacaaacg
    12181 aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc actctcgcac
    12241 ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc actcgtatgg
    12301 aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc ctctttttaa
    12361 atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct gcttggtgat
    12421 catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt gccaaagcga
    12481 atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt gttgcagata
    12541 aaccgctgac tcatcattgt tttttgccag ctgataagga agcagccctg ttcgctcatt
    12601 tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg aaaacagctg
    12661 caccaaactc tcatacgcag tatcaatcaa aatttcacgt ggcaattggc tttcactaag
    12721 ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat agtcttgaat
    12781 acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc aactagtttc
    12841 ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat gttcggacaa
    12901 catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga tccaaccttg
    12961 ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg gaaaactgtt
    13021 atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg ttcgttttcc
    13081 cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa atagcgataa
    13141 aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc actactatgt
    13201 aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc gccagatatt
    13261 ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc tcatcaacag
    13321 ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg tccagttcga
    13381 gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg tacactaagt
    13441 tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat cccgacttat
    13501 tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg accctatagg
    13561 acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac gatcacagtt
    13621 tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc atagctgcca
    13681 aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg taaatactgc
    13741 ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt gatcgataaa
    13801 acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc catgaagaat
    13861 acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc cattacgcca
    13921 cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt gaccttatcc
    13981 gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc gactgaaaag
    14041 ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac gagagcacca
    14101 ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc aaaaaataag
    14161 taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga tggttcgtac
    14221 gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat gatgactaag
    14281 ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct gagtgacccc
    14341 tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc aaactttcca
    14401 gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg tatgtttatt
    14461 tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct ttatacatac
    14521 tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca gggaacatcg
    14581 caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct gatacaaacg
    14641 gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc actaactcac
    14701 tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc aacccatatt
    14761 tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta acagggattc
    14821 tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca aagttcatcg
    14881 ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca gttgcatttt
    14941 cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct gaaagagcaa
    15001 ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg atacttttac
    15061 caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt ttcaatgctt
    15121 cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca acattcaacg
    15181 ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa gcttgcaata
    15241 agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct tgcgtaattc
    15301 cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca tctgtaccaa
    15361 agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct ttgacggcat
    15421 acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact aaccagccgt
    15481 cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct gctggaacaa
    15541 cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct gaataaccgt
    15601 aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt tccaatactt
    15661 tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa tctgcttcat
    15721 ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt tcatgatgtt
    15781 gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag ggtcatgtca
    15841 aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg ctccatcagc
    15901 aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg agctattgac
    15961 ttatccacgt gcttatcgat agccgtttat cggtttcttc attcttatga tagaccttgt
    16021 acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac agatggctac
    16081 aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat cgaaacgagc
    16141 aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta ctaaaccaga
    16201 tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt cacccatagg
    16261 tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca agaatgacat
    16321 agggatatag aaaataaatg taataatgat gaatacactt gaagataatg aacttaatcc
    16381 ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa ccacttggat
    16441 accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac cagctaaaaa
    16501 ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga tggacattac
    16561 taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca tgcttttacc
    16621 tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt caaaaatcgt
    16681 gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac caaaagtaat
    16741 aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac gaccgattgc
    16801 ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg attttgttgg
    16861 atctttttcg atctttgatg cataacgata cacatataca atagaaatag ctaacaaaat
    16921 aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa cagcttgtga
    16981 agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt gagaccctac
    17041 taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga ctgggatcag
    17101 taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc ctaatgcaaa
    17161 taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct tcacaactga
    17221 agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc caccaacaac
    17281 caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct cccccgctgg
    17341 tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt gaggattttg
    17401 ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac tatagtgccc
    17461 agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata agaccgtata
    17521 cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct tcctttcttt
    17581 tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt atcagaacaa
    17641 aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc cctattccct
    17701 cctctttttt caggttttta aagctttctt ctttttcatt gtagcgtttc ctttttttta
    17761 taaacaaaca ttttcgtctt ttaaaaggag aagaatttca catttgcaaa gaattgattg
    17821 atttttatgc ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt tacattattg
    17881 catgtcttgt atttttttcc aaaaaaaaag accaagaaat ttcttgatct ttttgatatt
    17941 tatttgactg ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa ccatggaaga
    18001 agttgcgcat gcagcactaa aaagacagca ctcacgatga atcctttgat caagttgaaa
    18061 ggaacgatcc caatcagtac atagtttgct aaagacatgc ccagcatctg tgttgcgttc
    18121 agccctaaaa acattaagta aagaggcgta ataacgaaat aatttgcgat actcataaaa
    18181 atagttagag cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc tttatgctgt
    18241 ttctttacac taaagaaata ataaatcggg aatgtaaaga tcgttgttga taagaaacta
    18301 gcagtgtctc ctaccaaatt atccggagaa aaacctgtag tcagcagatg taatactgaa
    18361 cgcaagaacg ctgttgcaac tccggccgct ggaccgaata agaacatgct gatcaatacc
    18421 gagatatcac taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg aaaagcaaaa
    18481 tactgtaaaa ctaatgcgat cgcagcaaac atcgctactg caaccatttt ctgtacacgg
    18541 gtgttcttca tttgaaatcc tcctgttagg actcctcttc agcgaacttt ttgacgtttc
    18601 tctccaaatg aaaaaactct attttccagg gaaaatagag ttagagttat ttgatcaagc
    18661 gtcgtcaaat aagctctatc ttctttatcc agactatact gtcggtatcg gaatttcacc
    18721 gattcagcta tttttttgca aaataggtcg tggactataa ccaccggtcg ggaatttcac
    18781 cctgcccctg aagacgaacc ttatataatt tttgtttaca tattcattat acatatctat
    18841 tcaaaaaacg caacatctaa cacttataaa aagtaagtgt tcattttgag tggttcagca
    18901 aactgctgac ttcttttttg ttcagttcac ggtaatcccc aggacgtagg ccttgtaaag
    18961 tcagatctcc gtatttttca cgttttagtt tttgcacagg aagaccaact gcttgaagca
    19021 tatttttcac ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata ctgtggtttg
    19081 tcttaggatc cactgataaa atttggaatt tagctggaga tgtttttcgt ccttcgatct
    19141 ttattccttt tgtcaaaggt gcaagcatcg attttgtagc gatccctttt acctttgcaa
    19201 cgtaaacttt atccacttca tgttttggat gtgtcaggcg ctgagcaaaa tctccatcat
    19261 ttgtcagtag caagatcccc gaagtatcgt aatccagtcg acctacagga tagatacgtt
    19321 cttttaccat cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct gcaacagcag
    19381 aaataactcc tttaggtttg tacaataaat agtaaccgta ttcttcttga taaattggga
    19441 cttcatccac ttccacagtg tcatgtttcc caacttttat ccctaattcc cttacaactt
    19501 gtccgtttac ttttacacgt cctgcaagga tgaattcttc tgcttttcga cgagaagtga
    19561 tgccagcgtg ggcaattact ttctgcaatc tctccattac aattattctc ctttattcat
    19621 tccatcaaag aatagatctg gtgacacgtc ttcttcaagt gaaatttcca tttcttgtgt
    19681 atcaggcagt tcttctaatg atttcaaacc aaaataatct aagaagtagc cagttgttcc
    19741 atataatatc ggccgacctg gtccttctac tctacctttt tcttcaatca gtttatgggc
    19801 agctagacgc tgtaccgctc ctgaaacctg aactcctcga atctgctcga tttcagctct
    19861 tgtgattggc tgtttataag cgatgattgc taaggtttcg acggcggctt gagataatgt
    19921 gtttgatgtc ccttgcgcat atcgcttaag taagggagcc agctcttttt tagtcgttaa
    19981 tacataatgg ctttccgttt caaaaatatg caaagctgag tgcgagtttt gagtgtaaga
    20041 ttcttttaat tctttcacta aacttgtgac ttctgcttcg ctgagattta acagtccgct
    20101 taattcttca ttactgatcc cttcctcgcc aacaataaaa agaagggctt caagttcact
    20161 taatttcgtc atgttcggta tctcctactg ctggataaag cataattgat tcatagtttt
    20221 ctttttgttg cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc ataaaagtag
    20281 tgacaacttc acttttagaa ggtgactcaa aaaaagaatc gaaagtacag ccatgcgcat
    20341 ttctttttat acgctcttcg ataaatacca ttcgttcttc gatcgttgtg tcatcgggcg
    20401 tgatcgtttt ttccactttt tgagaaaatt tcttacgttc taagattttc tgcatggcac
    20461 ggatcaaatc gatttttttg acttgtcctc gcttcaacgg cttttcagca tccgctaacc
    20521 catcgatgtt cataggttct tttgtgtaat aaagacttcg ttcctctgct ttttctgtca
    20581 gtctttcagc tgcatattta tatttacggt attctaaaag ctgagtgacc aattcttctc
    20641 gaggatcttc ttcccataat tcttcatcgt ctgtgacgaa ttcttggaca ggaagcaatg
    20701 tcttgctttt gattgccatc aatgtagcag ccatcactaa gtattctcct gcaacagcta
    20761 gctcgagtgt tttcattgta tggatataat tcatatattg ctccgtgatc gtagcgatcg
    20821 gtatatcata tatatcgatt tccatttgct gaatcagatg aagcagcaga tcgagaggac
    20881 cctcgaatac atctaattta atattgatct ccgtcaactc attcactctt tcttcttttt
    20941 ctttgctagc cattttccaa tgtaaggctc tgtgatttca gttgcacgga tggagacatc
    21001 cgggtacttt tcctgtaatt catctaacat ttgaaagccc atattctcac cgcgataaga
    21061 cggattcaaa gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt aaataccaat
    21121 aatcccttga atgttggttg aaccttctgg cgtaaagaaa aaaagttcat aatcgtcttc
    21181 agattcgtac gtatcaatct ccttcaacaa agaagtttta cctgtcagtt tcttatggaa
    21241 actcaatagt cccatagcaa ttttacggtt ttctttccgg taatgtgtta acatcttctc
    21301 accttcctat gactaagccc ttggaaaatg ctgcttataa acatccgtca ttcttttctt
    21361 cgtgatatgt gtataaatct gagtcgtaga gatatccgcg tgacctaaca attcctgtac
    21421 tgtccgcaaa tcagctccat tttctagcag atgtgtggca aagctatgcc gcagcgtatg
    21481 tggtgttact tctttgtaaa tcccagcctc tcgtactaac tgcttcagat ttttccagat
    21541 tccttgccga gaaagacctt tcccatgatt attgacaaat acatgcattt cagatgcgtc
    21601 ctttacaagt aatggacgtg cttcgtctaa gtagcgttcc agccattgga tcgcataatc
    21661 acctaaagga atgatccgct ctttatctcc ttttccaact gtctgtacca gtccaagaga
    21721 aagatgaaga tctcccaatt tcaatccaat caattcactg acgcgcatcc cagtagcata
    21781 catgacttcc aagattgcac gatctcttat ccctaacgtt tttgtcgtat caggtgcttc
    21841 aatcagcttt tctacttctg tcagagtcaa tgtacttggt aatttctgtg ctttcttcgg
    21901 cgtatcgata tgttgcatcg gatcatgatc agtgatacgc tcttgccgta aaaactgatg
    21961 gaatcttctg agacttgaga tcatccgagt aacagttgct gtcgcttttt tttcttcatg
    22021 caacttctcc agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg aagcaatttt
    22081 ctgctcctgt agaaaatgca agtactgttc caaatcacgt tcatagcttt gtcgtgtatt
    22141 ggcggacaat ccccgttcaa tattcaagta gtggagataa tcgttgattt gctctttcat
    22201 aataatcccc ctcatgcctc tctatcatat cagataccgc ttcattctga tagtcttttt
    22261 ccataatctg agcaaagaaa aagccgtcta gacactcaga cggactggtt tcttttttct
    22321 ttttcatagc attcctgaca gattccatgg aacgttagcc gatgatcttt tactaaaaaa
    22381 tgataacgtt cttctactac ttgctccact tcaagtaaaa gatcctcttc tacttcctcg
    22441 atcgtgccgc agttcaagca tagtaaatga tgatggaaat gtttggcgcc ttcttttctc
    22501 aaatcataac gggctaatcc atcattaaaa cttactttat cgacaatttt caattctgtc
    22561 aagatttcaa gggttcgata aacagtagca agtccaattt ccggactctt ttttttcact
    22621 aaaaagaaga tttcttctgc agataaatgc tctttctcat tttccaaaag cacaagcaga
    22681 gtagcttctc tttgcggtgt cagcttaaac cccgcttcat gtagctgctg ttttgttttt
    22741 ttcatcgccg aaatagcatt catgaccatt tactcaaccc caacaattta taataattac
    22801 aaataaaaaa agatataaca ctatgttaat taaaattatt ataagctagt taagaaaaac
    22861 atgcaaggga ttctcaatta gattttttga ctagttttgt cacaccgttt tcttgtgtaa
    22921 tcaatccttg tttcaaaaga ttacctaatg cgcgcttaaa tgctcctttg ctgataccaa
    22981 aagctttcat gatttcttct ggatccgatt tatctgtaaa gttgatttgc tgatctgcag
    23041 aacgttctaa aatggttaaa atcatttgtg catcatctga tatagcttca tatgctcttg
    23101 gcttcaatga aagattcagt acaccgtctg gtcggacacc aatcactcga ccttttatct
    23161 gctcgcctag acgaggttca ttatatcgtt cagaaggatg gataaaccca agatagaaat
    23221 cttccgttaa aacgtacgta cctgttaatt tcaagcggta tgctgttcct atcacatctt
    23281 tgtttttcat ttcttctgtt ccatatttgc ttaaagattt gaatattttt tcatctgcta
    23341 aactgcccca aatacggtct tttgcatcaa ctctaaggga aatcatcaag cgatcccctt
    23401 ttttaggcca tagctctgtc attgtcggca attcatcaag agatacgaca aagtctttat
    23461 ctggaagacc gatatcgaca aacgcaccta aatcttttcg tgtagcagta acgattccaa
    23521 aagcgtaatg accgatacgg cttttaggaa tttgtgtagt gatccgattt tcatgttttt
    23581 gattttggta accgaatcct tcaactgctt caccgatttt gtgttcacct tctgatttgc
    23641 ttaagccaaa ggtttgtccg tttttttgga caaagtagtt tttttcattt tcatcaataa
    23701 taatacctgt aaatatagtt gctaataaat cgttcatttt tttctccctc tatagtgaaa
    23761 ggttttctta atacatttct ttgctatcat aacataatgt tatctacttg aatagctgag
    23821 ccttggtttg gaaaggtgaa aaaaggagta tgacaacagt tgtgtcatac tccccttttt
    23881 cgaataaacg gtgttaaacg gcaggctcac agcctctttc atttgcctaa aattaattgc
    23941 tcccatttcc tccgaaccat ccagttttac agttgatggt tacgatgtaa aatggcaaag
    24001 cactttctcc aatgcctttc atcaatgcag attttgttga agtggcatat agattataat
    24061 agccttcccc attttcgatt ctggcttttt ctaatctgta acctccttca acgatatatc
    24121 cacgttggat acatgtagca atcactttgc tttggacacc tggtgcccac gtccatgctg
    24181 gattagatgc atcggcaata tcacttgctc tggcagattt gattggctga ttcgctgagt
    24241 ttgaagagga aggggtcgag gatgccgctt cttgctgtgc tgcttgtgcg accgcagctt
    24301 gttgagcggc ttgagctgca gctgctgctt gagcggcttg ttttgcttgt tcttcagctg
    24361 cttttttcgc ttcttctgct tttttctttt cctcttcttc ttttttctct ttatcagtga
    24421 gcatcttatc tacttgtttc aattgttcat tcaactgagc agatagatct gtatttttca
    24481 ctttttctac tgcttctttt gccgtcttgt attggtcgcg tgttgctgaa tccactactt
    24541 ttccgtcttt aaagactata gctgtttttt ctttagctgt ttgcaattca gcatattgat
    24601 ttttagcttc tgtttgtgct tgttgaatca attttccaaa atctgtgtta tccgtttcga
    24661 ttgcttggat ctctttatct gctttcaact tgatgtcatc tgctagatga tcatcgacga
    24721 tggcaggtgc tatgaaccac tcattcactt tttggatatc tcggatctta gattggatat
    24781 cttggtaagt tgtttctaac tctgaatatc ctttttcatt cttaagactg gataaatcat
    24841 tttttagttt tgataaatct tgatgaatca tatctgcacg aataaattga tggtgatcat
    24901 ctgtataaaa ggcagcaagt ttctcatgaa gttcttccat ttgttgctgt ctttttgctt
    24961 ctgctgctac ttcggcttct actttttgat gatggtcata ataagcccag cttcctccac
    25021 cgagtagaag tactgctgct aaaacgagat aaggtacttt ttttgatttt ttcttttgcg
    25081 ctttatctgg atctgttttt tctaagtttg attcttgatg tgttttttca gtatctggca
    25141 cgattgttat tgatttcgct tctgcttcaa acagcttagg ctggttctct tctttttcta
    25201 tttttgcttc aagatcagta ccctctttgc ttacagattg attttcagca tcggattcac
    25261 tatttatttc atcgggctct gttgactttt tgtctgtgtt ttcgagtatt tcttttttct
    25321 ctgcttccgg cagtttattc gttttttcct tttctaattg tttttcagta aatacttcag
    25381 ttttttctgt gatatccgtt tttacgtctt ttatttttac tgtttcttta cctgtggcgt
    25441 tttctctatc agagtctttt tcagtttctt gttccttttc tggctctact ttgttttcct
    25501 gtgcaatttt ttcactgatc attttgagca gctctttcgc gtgctcttct tttgtatctt
    25561 cacgatgtgc tttgatataa gcggatagta tcgaattttc ttcctcatta tcgacgattt
    25621 ctttcagttc agctactctt cttttttgtt ttgattcaag cgtttcttct tgattctttc
    25681 ctgattcttc cgaagtctca tgaacagttg ctttcttctg ttcatttttc tctatattag
    25741 ttacttgatc gtgagcaggt ggttcttcgt cttctggttc tgctttttcg aataattcca
    25801 ttacagattc tagaggtaaa tctttcaatt ccgaccattc gattggatcg tttaattctg
    25861 tatcaggaaa gactgcatct tcagttgatt tatcctgcat agctgcatct gtatttgttt
    25921 cttcaattga ttcttttatt tgctgatctt ctgcagttat ttgatgaccg cagtctggac
    25981 agatagtatt ctgctccatt tttttcgatc cgcaatttgg acattttttc atcacttagc
    26041 gatcggctcc tttactaggt ttacgctacc atttaatcta tcatattcta tacgtcacgc
    26101 caatagctta tttttaggat ttacttaaaa ataatgaatg aatttccttc ctatagaaaa
    26161 aaaagcttga gactgcgtag tctcaagctt tttttctttt aaaataaacc aaacagtttt
    26221 actgatttct cttctggtca atagtccacg cttattctaa gaaatctttt aattgttttg
    26281 aacgagaagg atggcgcaat tttcttaatg ctttagcctc gatctgacga atacgttccc
    26341 gagtaacacc aaatactttc cctacttctt ccaatgttct cgtccggcca tcatctaaac
    26401 caaaacgtaa gcgaaggaca ttctcttccc gatcagtcaa tgtatctagt acatcttcca
    26461 attgttcttt tagtaactca taagctgcgt gttctgctgg acttgttgct tcctggtctt
    26521 ctatgaaatc acctaagtga gagtcatctt cttcaccgat tggcgtttca agggaaacag
    26581 gttcttgcgc gattttcaag atttctcgga ctttttctgt tggaagatcc atttccgcac
    26641 cgatttcttc tggcgtaggt tctcttccaa ggtcttgcag caattgacgt tggatacgta
    26701 tcaatttatt aattgtttca accatatgaa ctggaatacg gatcgtacgt gcttggtcag
    26761 cgattgctcg agtgatggct tgacgaatcc accatgtagc ataggttgaa aacttaaatc
    26821 ctttgcggta gtcaaatttt tcaactgcct tcatcagtcc catgtttcct tcttggatca
    26881 agtcaaggaa ctgcatgcct cgtcctacat aacgtttagc gatacttact actaaacgaa
    26941 ggtttgcttc tgccagacgc tgtttcgctt cttggtcgcc ttcttcgatt ttcaatgcta
    27001 acgctacttc ttcttccgca gttaaaaggg agacacggcc aatttctttc agatacatac
    27061 gaaccgggtc attgattttt acaccggttg gcgcagaaag atcttctgtt tttgcttgtt
    27121 cagctttttt ctcagcactc ttcaagctgt gaattgatgg atcaccattt tcatccacca
    27181 cactgatacc tgcgtcttca atcttttgga tcaatttgtc catttcatct gcattcaaag
    27241 tgaatggtgt agcaagctga ttggataaat catcatatac aacttgtcgt ttgggtttgt
    27301 tttctttgat gaaagctgct actgctgctt catacgtttt tttatctgtt ccatttgcca
    27361 taaaagaagg ccccccttga gtagctattt ggcttgtttt aactgtttcg ttaaattgat
    27421 gatttcaatc gtcaattcaa gctcaagctg tttgtttcca gttcgtctgg cttcttgctg
    27481 catttgtttt tttaatgtta tttcatcagc taagccagat ttgctgatca catctagcaa
    27541 gtcttggatt tctcgtggcg aactttcttc tgccaccggt aaatattcga tttccaccac
    27601 caaccgcctt aaattttcat cttgcaaaaa gtctaaaaac tctgcaaggt tgaattcata
    27661 atgttgtgag acatatgcat cgaataaaaa ataaacttct gcatatgcat catgaataaa
    27721 ctgaaattct cgttctttta aaatattacg gacagaaact tctttaaatg cacgatataa
    27781 tagaatctgc tctgcctttt ctacttgcgt caaaggtctc tttttccgtg cagtttcttg
    27841 tctcgtaacc tgagtgtgtt gactttcctg aggttcttga cgattttgct gatttgtcct
    27901 tcgaagatcg cgcagttgtt tttgtatcgt ctctctgccg atctgaaact ctgttgacag
    27961 ttgagataga tacatatctt gttcgattgg tgaaacaact cggctaagtt caactagtaa
    28021 ttcttcgagg tattcgatct tgtctttttc attatctaaa tttttccctt gttttaaata
    28081 acgtgattta aaagtaaaga ctgtttcgcg cccatgagcc actaattctt ggaaagatgc
    28141 ttctccatac ttacggagat attcatcagg atcaagcttt tctggaacga ctacgacaga
    28201 aagtgctaat cgactgtttt gccgaagcaa ttcaatccct cgattggttg cttcaaaacc
    28261 tgcgttgtct ccgtcatagc aaaagaccaa ttctttcgtc agcttttcca tttgttggat
    28321 ctgttgagct gttagacttg tccccataga agctaatcca gttttgatac cagcctgcca
    28381 tgcagcaatc acatccataa acccttcgaa caacaaaatt tctccgtttt tccggatatc
    28441 gcttcttgct ttatcaagat taaataaaac ttgtctttta ttgaacagtt ctgtttctgg
    28501 actattcaag tatttgggct gatctttttc ctgccctttt tcaggtgcaa gccagcgtcc
    28561 tgaaaaaccg atcgtctggc cttgagaatt acgaagtgga aacatcactc tttgataaaa
    28621 acgatcagac atacggccat catcatgcgt gacaaacaat ccagagcgtt ctaacaattc
    28681 atttgaaatt gattcattct tgaatacttg ctgaagaaaa gaacgctcat taggtgcaaa
    28741 acctatgttg aattcttcaa ttaattctaa agacaaccca cgactttgca aatattctaa
    28801 agctggctgt ccagcttttg tatgcaatag catgtgatga tagatttccg ctgctttttc
    28861 atgagcagca atcagttttc ccgtagcaga ttcaggattt tggacagcta tagtacgctg
    28921 acgccattga tcttctaacg gtatctgttc gaagtctgcc acttttacca ctgcttctgg
    28981 aaaacttaga ccttctagtt cttgaagaaa agtaaacacg tttccgcctt taccacaacc
    29041 aaagcaatga aaaatctgct tgtcttctgc aacagaaaaa gacggtgtac gttcttcatg
    29101 aaaaggacac agccccaagt aatttttacc agactttttc aactggacat attgtccaat
    29161 cacctcaacg atattcgttt gactgcgtac tgtttcgata acctcttgag gaatccgttg
    29221 agccattttt tcaccccctg ctctattgat atcttttggg tttaacagaa gatatttcag
    29281 gagaataacc ggtaaaaaat cgcactaact gaaatcagtg cgattttaaa ggacacaatt
    29341 atacatgttt gattgtatca tatttcattg ctttttcaag tataacacat ttctatctca
    29401 ttaagcatca atttgctact cttttcagta actgattttg aatatcagcc ttccagtaca
    29461 atactcgaag aacaatattt acgagcatga atacaaagaa gtatcctacg ctctctccca
    29521 accctaagtt tacttccaaa attccatgga acagcattgc agctacatat atccctacga
    29581 tcgtcgttac tgctaggaag atgcgcaatg ggttgaaggg ataacaagct tttacaactg
    29641 ccatgcaact gatcccaatc agcaagtaat acatcaaagt agtagtttca gcatttcccc
    29701 atgcgaatgt ttttccaagt agatacaccg caatgatatt cactactaca agcaaggcat
    29761 ttggtaaagc attaataagt gctgtaggca aataacggta agtgattttt cttttatctt
    29821 gttcaaaaga taaaaagaag gaaggatacc cttcaatcgc taaatcaatc agcgtgattt
    29881 ggatagggat aaacggaaat ccaattgctg taacggcaca tataatcgat aaaataaatg
    29941 aataaatcgt tttgataaaa aagatgcctg agactttcgt cacattgttg accactcggc
    30001 gtccttcaaa caggacttct ggcaatgtag taaaatcaga gtccaacaat accagattcg
    30061 caatctgacg tgttgctcca tcaccttctg ccatagcaat gctgcaatct gcttctctta
    30121 acgccaaaac atcattcaca ccgtcgcctg tcattgcaac cgttctgccg ctttctttca
    30181 attcatttac cagcaatttc ttttgctgcg gactcactcg tccaaaaacc gtatagcgat
    30241 gagcagcttc tcgcacttcc atttcttcag taatttgaga aagatcgaca tacgcatcat
    30301 aattcggtaa tcctgcacgt ctagcgatat tggagacagt aaaaggattg tctccagaaa
    30361 tcactttcag atccacacct tcctcatgaa gataagctag tgtttcttga gcgttttttc
    30421 ggattggatc atcgatttca aacagagcaa ccggctcaac aaatgctggt ttttcttcgt
    30481 tcaattgctg atttggagcg atccccaaca taagtacgcg atagccattc gcttgggctg
    30541 tttttagact gtccggaagc cgatcttctt ccaccaaacg ttcaggcgct cctacgtaca
    30601 cgactcccag ttctggaaat tccatcgcgc cccatttgcg atcagaagaa aatgcgactt
    30661 tgtttgtcac atcataccga ttagaaagct caaaataatc tctcatagcc cgcatagtga
    30721 tgttgttatc actgctttct gccagatacg tccccataat ttctgggatg gctttctcgt
    30781 acagcggatg tagtgcttct acccgctgga tactcatttt cccttcagta atggtccctg
    30841 ttttatccaa gcataatgta tcgacatggg ccagcgtttc aattgaatac atgtcttgaa
    30901 caaggattcg tttttttgcc agtttggtta cccctgtcgt taatgcaata cttattagca
    30961 ataccaaacc ctttggaagc atgcctaaca aagctgctgc agatgcaata acagaaattt
    31021 tgatagaagc atcgcggatg aacagtgctt ccaaaaatag tatgatcccc aaaggaataa
    31081 tcacgaaact agtgaatttg gatactttgc gaatcgaatc tacaagttca gaacgaatag
    31141 ctttgtgagt ctttgcctcg ctagtcaagc ggacagcata attatctgca cctactcgga
    31201 taacttcagc catgacagaa ccgcttgtca aaaagcttcc agaaagcaat tcagctccag
    31261 tttctttgac aatcaaatca gattcgcctg tcaaaagagc ttcatttgct tctgcccgtc
    31321 cagatataac ctgtaaatcg gctggtatct gttctcctgc tccgattttt accaaatctc
    31381 ctaataccag ctctttagta gaaatcgatt gttctttacc gtcccggatc actagtactt
    31441 ctgattggga aacaatcgat aatttccgta caagatttct tgcatgtatt tcttgataaa
    31501 ttccaataac gatattgacg acaataattg ctaaatacgc catgttggaa tacgcaccta
    31561 caaataacag acaaatccca ataccaaagt tcagaaaatt gaataacgtc aatatattat
    31621 cgctgaatat ttgttttgta ctttttgatg catcttcctg gtagtcattt tgctggccag
    31681 aagccatttt ctgaactacc tcttgctgtg taagtccatt cattttcttt ttctccattt
    31741 tccgccaact ttcaaaaata atcacgtgtt taagtctagc gtgttccaag attaaaaaca
    31801 acttgttttt ataaaattta tagaaaaaca cacgaatgat tttacaaaaa agatgccaac
    31861 ctttttgttt gaatcacccg tgtgctgttt ttattatcaa tctatcttta cttatattgt
    31921 tcctccaaac ggctcatcca ccaacctagt gctgtaccgg ctataaaaag aacaggacaa
    31981 gctagtaccc cagcaactcc caatcccgcg taatcggtag gaatgatcat aatcgttgaa
    32041 gcaaaaacta cacctaaaat gaaatggaat agcttgctat atgcctttga gaaaatgtaa
    32101 tccatgattt ttgacaatcc aagaacacaa agaatcccac caattccgat cggcacaatc
    32161 acttcaagtc gtacttcttt aaatccgtca gccatcgctt tatacaatcc catatacacc
    32221 aaaaaattag aagggctcaa tcctgggacg atcataccca aaccaatcaa tccgccggca
    32281 atcagccatg ttccaaaatt ctgatcgact tgcccgtcaa ataattgagc gccgtataat
    32341 aaaaacaaat aagccagaac aaaagtaaca accatgatga tcacatcaga agtatctctt
    32401 ccttttttcc ctgcttcttt ccataacgca ggaacagttc cagcgataca tccgacaaaa
    32461 aaccataata tcgttgtttc atacgcacct aataaaaaac tgactaaaaa agaaagtaga
    32521 aagatgcctg tcactccgcc tagaccaaca ggcagaaaaa acaaaacatt ttctttgaaa
    32581 ttttttgtaa ggtgggctaa aaaagaaatc agccgctcat acatgccaaa gacagctgct
    32641 aatgcaccac cgctgactcc tggcaagata aatcctgaac cgataaacat tcccttcaca
    32701 aaccgtaaca tccagtcttt ggttgtgtac cccacttctt gattttccat tttttgcctt
    32761 ttttccacta tttgcgtact ccttctttca tttaatttta ttcatgattg aacatttcct
    32821 gctattttac ttaaagactt tttcataggt ctctattatt ttttcattca ttttgacaat
    32881 atcaggataa aacatgttac ctccattatg atagagatag ccgccattgt aaagcaagga
    32941 ctggatccgc caatatcggt attgtttccc ttcctcattt cctaaaagtg ggctcagcac
    33001 atctttagaa tacgcttctg ctaattctac tgtgttttct ccgccatgat tttttacata
    33061 ggcaatataa tctttcccaa aattatatgc ttggacagct gtccataaat cgcatccttg
    33121 tttctttgct tctactatca tttccgccaa atatttgaca ccttgatcaa tactgtcagc
    33181 tggattatca atgatctcag cttctccatg cagactttcc gagctctgca tgagatctgt
    33241 cccttttcct tttgattcgg tcaatattat cgccttgatc aaattctcgt agttttccat
    33301 atgataattt tttgcagctg ttgttatttc tatttgatag ctttccactc gttttaccga
    33361 acgataagtt cttcctcata aaaacaacac cgcaccaata accgtcagaa tgatcatgta
    33421 aaaaagaata cggataatag ataatcgttt tcgcttcatt tcatcaccta gttattttga
    33481 catctttttt tatcttacta tttctttatc ttcttggcta tttcttcctt gatttctgtg
    33541 tacgccttaa gaaaaaatga attttcctgt attttttgac atcttacgaa aatgatcgtt
    33601 gtttttcagt aaaaacaatt gcccaatgac tgatataatc gttatacttt acacagtagg
    33661 tgattatgtg aaaaaagaaa agaaatatgc aaaaatgcca gatgggagcg aaatttacta
    33721 tgaaaagagc ggtcaaggct tccctctctt tttactgcat ggcaatgacg gcagcggccg
    33781 ctttttttca gaacaggtcc ctgtactcga acgctactat accgtgtatc tagtagatag
    33841 ccgaggacat ggtcgttcga caaacgaagc atctatgttg aattttcagt taatggccga
    33901 ggatctgaat acgatcatgt tgctggaaaa aatcgatcag gcagattttc ttggatttag
    33961 tgatggagca aatctagcac tggtatttgc cagcagcttc cctaaaaaag ttcatcgatt
    34021 gattttaaat tctggaaaca cgcttgttaa aggcgttcgt ttttctgcca gagtcatcag
    34081 taatattcat tatgcatggg tatggttact ctctttattt cgcccaagtc ttcgcaaaaa
    34141 tcttttagtt atcaaacttt tgctgcatga catcgggctt acagaaaatg atttaaaaaa
    34201 aatcaactcc cccactttga tcatcgtggg aaaaaaagat gtaatcaagc taaaacattc
    34261 tttgtatata gctaaaacta taccaaaagc ttcctttgtg cttgtgaaag aacaaggaca
    34321 tgaattagct agaaaagatc ctgaacgctt caatcgagaa gttcttcaat ttctttctga
    34381 aacataacta ttaggaatag aaggtgattt atttgttaaa aaataccata caatggctga
    34441 aaaaccacct aggactgttc aaaacaatct ttttgatttc tgtcattgtc attattgtcg
    34501 gtgaattgat gtccatcggt aaaactctat ctatccagca attaggagaa acgtttgcca
    34561 ctatacctgt atggaaaagc ggactaatgc tattgattgg tcttatctct gtgttaccta
    34621 tggttggtta tgatattatt ttgaatcgga tgcttgaaca aaagcagaat cctcgttacc
    34681 tgtttgagac aagctggctg atcaatacga tcaacaatat tgccggtttc ggcgggtttg
    34741 ttagtgtggg gcttcgctct gaactatatg gacaaaagaa agagagcaga aacgtgatcc
    34801 aagcattatc gaaagttttc ttgtttttga tggctggact ttctatttac agtctgcttt
    34861 cttttttact tgtagtcttc acgccagttg ctcctttttt gaagcaatat tggatatggt
    34921 tgatcggcgg cggtctatat tttcctgtcg tctttttatt tactactttc aaaaagaaag
    34981 gattccttgg tggtacctca cttaaaactc aaggacagct attgctagtt tcattgttgg
    35041 aatggaccgg tgtcctgttt agtttccttt ctgttggtta tttaatggaa atccggattg
    35101 atctgtggca aacaattcca ttattcattg ctgcttcagt gataggaatc gtgtctatga
    35161 ttccaggaga aattgggagt ttcgatgtaa tgatgattat cgggttgtcc gctattggtg
    35221 tgccaagaga aatagtcgtt gtgtggatct tgctctatcg cctgttctac tacatcattc
    35281 cgtttttgat tggaatcgtc tttttcttca aaaatatcgg ttccactttt gatcagcgtt
    35341 attctggtat ccctaaacaa ttagcaacag aaattgcgca taaaatcgtg gttgtcttat
    35401 tatacttttc tggaatcatg ctagtacttt ctgccactat cccgcaagct tttacagagt
    35461 ttcgctggct tcatagtttg aacccattga aatttcattt tattatccaa tttcctagta
    35521 tactattggg atttctttta atcattatgg gcagagggat cgcagcacgt gtgaaacgag
    35581 catatttgcc tacaatcttc ttgattgttc tagctttact ttatgtttta ttaagtgatt
    35641 ttagtttcac accggtggtt ttcttgtcta ttctgcttct gatcattctt gcttctaaaa
    35701 acgaattatt tcgggaacaa ttgatctatt catgggagtg gcgtacgatc gatggaatca
    35761 ttattggtgc actgtccttg ctttacatcg ttattggggt atacaatctg ccggatttcc
    35821 ctcatcggcg tcaccatttt atttcctttt tcttatttcc ttctgaaaag atttggtttt
    35881 cgggattgtt ggccatcatc gctgttagtt tcatgatcgt tctatttgtt catttccttc
    35941 aaggagaaaa gaaacagatt ggcgaagcct ttaatgaaga aaaggccctg aagatcctta
    36001 caacttatgg aggaaactcc gatagtcaat tgatcttctt aaaagataag cggatgtttg
    36061 cctatgaaaa agacggtgaa ccaactgttc ttctccaatt cgcttgcttc aataataaat
    36121 gtattgtaat gggtgatcct tctggtaaga aagaagattt tccagaagca atcgaagctt
    36181 ttatcgaaga aacagatcga ctatgctatt taccagtttt ctacgaaaca agcgaagaga
    36241 ttgttatgat tttgcatgaa ttcggctacg atttcattaa gatgggggaa gaagcttatg
    36301 tagacctgaa tagttttaca acttcaggga aaaaaatgaa gggcactcgt gctgtcttga
    36361 atcggatcga acgagaagga tttacatttg atgtcttaca accaccattt tctactgagc
    36421 aaatgagtac tttcaaaaat atctctgata attggttggg ttcacgaaag gaaaaagggt
    36481 tttctttagg ctttttctct gaagattatc tacaaagagc gcctattgca gttgtcaaaa
    36541 atacccaaga agaaattgtc gcttttgcaa cgatcatgcc tacttacacg aacaataaag
    36601 taggaacgat tgatctgatg cgatatgatt ctaaaaaagc accttctggc agtatggatt
    36661 tcttgttctt gcatttgttt gcctatatga aagaagaaca tatccaatgg ttcaatttag
    36721 ggatggcccc tctttcaaat gtcggaactt ccagaaagag ttttttacag gaaaggattg
    36781 catacttggt ttacgagttt ggttctcatt tttattcttt ccatggatta agagaatata
    36841 aaagcaagta cgctacaaac tgggtctcac gttatactct atattctaga gatagttgga
    36901 ttgcatatgt tatgattgct ttgctgatta ttgataatgc gccagttgag cagacacaag
    36961 gtagaataag tggtttgaaa aaatggatac gaagaaagta ttaaaaactg cataccgata
    37021 gatgaacact ctcatgatga ataaaagaga ctgaagtaaa acgaaagttc atgcttctac
    37081 aatctcttct tatacaattg aagaacaaag agcggtccaa acagaaatcc tgtttggacc
    37141 gctcttttat tttaaacatt tttcattacc aaaatattcg agtcgcgttt cttatgcttc
    37201 gatctctgta acgatacctg aacctactgt ccgtccgcct tcacgaattg agaatgtcgt
    37261 tccgttttca acagcgattg gatggatcaa gtcaacatca atcgtcacgt tgtcaccagg
    37321 catgaccatt tctgtatctt ctggcaatgt gattgttcct gtaacatcag ttgtgcggaa
    37381 gtagaactgt ggacgatagt tgttgaagaa tggagtatga cgtccacctt cttcttttgt
    37441 caacacgtac acttctgctt tgaattttgt atgtggagta atggaacctg gtttagcaag
    37501 tacttgtcca cgttcgatat cgtcacgttg gatcccccgt aacaacacgc ctacgttatc
    37561 cccagcttcc ccgtaatcta acgttttacg gaacatttct acacctgtta caactgcttt
    37621 ttgtgtttca ggtttgatcc ctacgatctc tacctcatca ccgacacgaa cagcaccacg
    37681 atcaatacga cctgaggcaa cagttcctcg acctgtaatt gagaagacgt cttccactgg
    37741 caatagtaat ggtttatctg tatcacgttc tggtgttggg atatattcat ctactgtatc
    37801 catcaattcc ataatagcag cttcagcatc tggatcgcct tgcaatgctt ttaatgcgga
    37861 acctttgatc acaggagtgt catcgcctgg gaaaccatat tcgctcaata actcgcgaac
    37921 ttccatttct accaaatcga tcaattcttc atcatcgact aaatcaactt tgttcaagaa
    37981 aacaatcagg tattttacac caacttggcg tgacaataaa atgtgttcac gtgtttgagg
    38041 catcggaccg tcagttgcag aaacaactaa gattgcacca tccatttgtg ctgcccctgt
    38101 aatcatgttt ttcacatagt ccgcgtgtcc tggtgcatca atatgcgcgt agtggcgttt
    38161 ttctgtttca tattctacgt gtgcggtatt gatcgtaatc ccacgttcac gttcttctgg
    38221 agctgcatca atactagcat agtcttgagg atttgctagg ccttttttgc ctaatactgt
    38281 agtgatcgca gcagttaaag tagttttccc atggtcaacg tgtccaatcg taccaatgtt
    38341 aacgtgtggt ttacttctgt cataatgttc ttttgccata atttacaaac ctcctaaata
    38401 atatagatca ctgaaaaatt tcagctctca cctttttatt atagtctttt tttgactaaa
    38461 tcaaaaagaa tacgtccgac ttactttttg taagattcga actaagctta ttatacgtca
    38521 atattggtca aagtcaacta agagcactcg tctgtcttat acgcgaaaaa agtacctttt
    38581 ataaataaaa aaatgtgttt tttgagaaaa acttcaagat agtccttaac tttcttttga
    38641 agcaataaga acaaaatgag gaaaagttta aaattcagct gttgccaaca actttcaata
    38701 gacgaaatct tctatttcgt taataataag gaaagaggtg aagtacatga aaaaattaat
    38761 tggcgtactt gtcgttttag gcatatttct cggcggaagt tttgcagctt atcattattt
    38821 ttacggcggc gaagcttatt atacaaaaat cacgacgaat ggcgaaatgt cagctaccca
    38881 agcagataac ggagagaaat tcacgactta tacctacaag caagcggctt ataataaaga
    38941 tggtgaaaaa aaacaggtga cgcttcgcga agaaagagaa catccattaa agatgaatgc
    39001 gtatcttaaa ctcaaagtca atcctcgtaa aggtgtatta agctgggaag aaataaaggc
    39061 ttctgaagtg cctaaaaatg cggcagaaaa aatcgataat tagtaaatgg tcttaaaatt
    39121 ttattagata aaaagaggct gggacagaag cgtttaactc caagaaataa gaaggagttg
    39181 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    39241 tctcacgctc tttttttgtt atattcgtct tttccgatcc ttctcttgac acaattcatt
    39301 taactgatca ctggtacttg aacacctaat attctagtca tttttataaa cgattcaata
    39361 cttcatccac atttttgatc atcacggata tcgtaccatc cggattattc acaaattcaa
    39421 tcaaatctgg gttgcgatat acatctactg gtacgatcag ttcgatcccg tttgatagct
    39481 tgaatttttg ttttccaaat tttttctcag aaatttcttg tacttctttt acaggaggtg
    39541 cttctgggac aaatccttgc tcttgtactt cttctttaaa agccattttt gccgtgatgt
    39601 tttccttgaa gactttttcc gcaatcatct catgatccaa ccgacctttt tcttctatcg
    39661 tatcatagac agcttctttc aaatcagcca tgatatcaaa ttcttctgtc tcaaattttt
    39721 ttccaagatg cttggctact ttttggatca cttttacatt ttcttctaaa gaaggtgcag
    39781 gaacactttc gatcactctt ccagaaaaat aaagctcttt tttgccagaa aactcatatt
    39841 ttttttcaat taattcgtag gtccaatcag tcaaattcac agcgaatgct tcatctgctt
    39901 tttgtgtttt cgcccctaga attgctcgat tcaaaatcaa gcggttgtcg atccctgctt
    39961 cgtctgcttc aacaaagtga gtgtaagcct cacgataatt gactttcaat aaagataagt
    40021 acatcactgt atctaattca tacaagacaa caaatacatc tgcactaggt gcttcttcac
    40081 tttcttggta gatatcgaac caccgctgaa cgattgcttc acttgcttcc acaaaatttt
    40141 cttgagcgat tttaagtgat tcaacgatca tgctttcttc tgctagttgt ccagtttttg
    40201 tttgagcaga agagattttt tggatcttct tttgaagaaa atcacgaata tattcttttg
    40261 tcagatccag ctctttttgt gaaaagactg gagaaccagt ttctctgtca ataatatgca
    40321 gaattgcttt ttttaaatag atatccattt tctcattccc ttctgtctta gtttacggaa
    40381 aaaaaatcaa aaataccaga atcaaaatca ttttctttta aacaaaaatt tcaagagtag
    40441 aaacaaaatg gattcttttt ttcgaaaaat gcgctacact tatagttgaa cgaataaaaa
    40501 aggagctgaa agtatgaatt cattaacaag cacttatcgt ctatcaaatg gttacgaaat
    40561 cccggtagta ggattcggca catggcaaac acctgatggg gatgtcgctg tttcctctgt
    40621 taaagaagcg ctggctgcag gttatcgcca tatcgatact gcccaaggat acaagaatga
    40681 agaaagcgtc ggccaagcga ttaaggaaag tggaattcct agagaagaga tattcttgac
    40741 aacaaaatta tggaatgcaa accacagcta tgaacttgtg atgtcttctt ttgaagaatc
    40801 attgaagaag ctccaaacag attatttaga tctgtttttg atccactggc caaaccctgt
    40861 tgcttttcgt gataattggg aacaagcaaa tgcagataca tggcgagcat ttgaagaact
    40921 gtatgaagca ggtaaaatca aagcaatcgg tgtgagcaat ttcttacccc accatctaga
    40981 tacactagca aaaacagcaa aaattatgcc gatggtcaat caagttttcc ttgcaccagg
    41041 cgaattacag acaactgtag ttgagtatgc aaaaaaacat gatatgatcc tcgaagcata
    41101 tagtccatta ggcactggta aaatcttcga tgtgcctgaa atgaaacaaa tcgctgaagc
    41161 acatgacaag acgattgcac aagttgcttt gcgttggtcg ctgcaacacg aattccttcc
    41221 tcttcctaag tcagtgacac caagcagaat caaagaaaat actgaattat ttgattttga
    41281 attaacagaa gaagaaatga aacaaatcga tcagttagat ggtgtagtcg gcaaagcaaa
    41341 agacccagat actacccaat tttaagtcga aaggtgattt tatggatcca atcgaacgct
    41401 tgaattctct ctcggaagaa gttacccaga cttttcattc cgattttgtc tttttgatcg
    41461 atgcagagaa aatccaacat tttcctgcaa gaaactggac acatgatcaa atcatcgaag
    41521 aactgaaaaa acggttcgat cattcgctaa tggtcaaacc ctggcacgaa catgaagtca
    41581 tttattcacc tgaactacct gtttttgcct tgataccgaa aaaatagaaa gaatggaacg
    41641 gattatgtat atatacataa tccgttccat tttttatttt tctttcaaaa aacgggaaaa
    41701 tacgcaaatt tttctgttgt aatttgcgta tacgcaagtt tttttgcatt actccttttc
    41761 atttggctgt ttttttctta taatcgaaag ggtatgagcg aaggagagaa catatgaaga
    41821 aaaaacagaa acaaaaatta ttaaatcaat tccgtccttc attggatggt gtaagaggac
    41881 aattattccg ttctctagaa gaagagagct tgcgacgtta tggtttgccg ttacaagtca
    41941 tgcttgatcc caaaaaccgt caaatcttaa cgatcggatt agctgggaaa acaaatgaag
    42001 atacacggat caacgtgcca atcgatgaca acttcaatac tgtattaaag agaattcgtt
    42061 caggagaaaa cgggattttc gaacgattcc gtgacaatct tcttattgaa atcgtgtctt
    42121 actggaatga tcaacgattg aaaaacgatg aaccaactgc gcaagctgtt gcgacaccag
    42181 ttacagaaga aacagtcagt gtgccagaag aaaaaacgga aaaaccaaaa gcggacacaa
    42241 ctgaaaatac acgctcaact gatacgaatg ccgctttaag ttttgctgat ttttcaaaag
    42301 ccgttgctga gtatcctaaa ttttacacag aacaaaatga acatgaagta gttattaatg
    42361 aaaaaaatgc tgatgaacct cgtaaattag caactatttc tatgactact gaaaatgagt
    42421 tcgttatcga aaaagcactc gaacggaaat acaaagtcaa attgactttg attcctttga
    42481 tcgaacaatt tgcagcgaca gcgattactg atcgataaaa attctatttg tcataaaagg
    42541 ctgggattta aaatcccagc ctttttagtc atataataat ttctacagca agtaaaaaga
    42601 aattccttct aacatcaggc tcaaatatca gcttgacctg tccaacaaat cacctatgat
    42661 cctacaatgt taggcgactt ctcttctacc tgaagatatc ttggagttgc ctgatttctt
    42721 aagatgccta accttaacta taaaaaattc taataataaa catattttct gatagagaaa
    42781 aaag
//
LOCUS       DAZAFF010000012        42293 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000012 DAZAFF010000000
VERSION     DAZAFF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.012"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..653)
                     /locus_tag="ACG9BB_000651"
     CDS             complement(<1..653)
                     /locus_tag="ACG9BB_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2836622.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVE"
     gene            855..1154
                     /locus_tag="ACG9BB_000652"
     CDS             855..1154
                     /locus_tag="ACG9BB_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2836623.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            1419..2294
                     /locus_tag="ACG9BB_000653"
     CDS             1419..2294
                     /locus_tag="ACG9BB_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836624.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            2291..2509
                     /locus_tag="ACG9BB_000654"
     CDS             2291..2509
                     /locus_tag="ACG9BB_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836625.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            2496..5123
                     /locus_tag="ACG9BB_000655"
     CDS             2496..5123
                     /locus_tag="ACG9BB_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFN2836626.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            5138..5356
                     /locus_tag="ACG9BB_000656"
     CDS             5138..5356
                     /locus_tag="ACG9BB_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836627.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            6875..7189
                     /locus_tag="ACG9BB_000657"
     CDS             6875..7189
                     /locus_tag="ACG9BB_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836628.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(7242..8015)
                     /locus_tag="ACG9BB_000658"
     CDS             complement(7242..8015)
                     /locus_tag="ACG9BB_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836629.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            8120..8365
                     /locus_tag="ACG9BB_000659"
     CDS             8120..8365
                     /locus_tag="ACG9BB_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2836630.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            8521..9372
                     /locus_tag="ACG9BB_000660"
     CDS             8521..9372
                     /locus_tag="ACG9BB_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFN2836631.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            9405..10082
                     /locus_tag="ACG9BB_000661"
     CDS             9405..10082
                     /locus_tag="ACG9BB_000661"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFN2836632.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            10570..11535
                     /locus_tag="ACG9BB_000662"
     CDS             10570..11535
                     /locus_tag="ACG9BB_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFN2836633.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            11557..11769
                     /locus_tag="ACG9BB_000663"
     CDS             11557..11769
                     /locus_tag="ACG9BB_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFN2836634.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            11844..12320
                     /locus_tag="ACG9BB_000664"
     CDS             11844..12320
                     /locus_tag="ACG9BB_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFN2836635.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            12317..14125
                     /locus_tag="ACG9BB_000665"
     CDS             12317..14125
                     /locus_tag="ACG9BB_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFN2836636.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            14202..14417
                     /locus_tag="ACG9BB_000666"
     CDS             14202..14417
                     /locus_tag="ACG9BB_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFN2836637.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            14509..15372
                     /locus_tag="ACG9BB_000667"
     CDS             14509..15372
                     /locus_tag="ACG9BB_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFN2836638.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            15409..15945
                     /locus_tag="ACG9BB_000668"
     CDS             15409..15945
                     /locus_tag="ACG9BB_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFN2836639.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            15959..16381
                     /locus_tag="ACG9BB_000669"
     CDS             15959..16381
                     /locus_tag="ACG9BB_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFN2836640.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            16311..18710
                     /locus_tag="ACG9BB_000670"
     CDS             16311..18710
                     /locus_tag="ACG9BB_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFN2836641.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            18742..20733
                     /locus_tag="ACG9BB_000671"
     CDS             18742..20733
                     /locus_tag="ACG9BB_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFN2836642.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            20756..21007
                     /locus_tag="ACG9BB_000672"
     CDS             20756..21007
                     /locus_tag="ACG9BB_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFN2836643.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            20997..22226
                     /locus_tag="ACG9BB_000673"
     CDS             20997..22226
                     /locus_tag="ACG9BB_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFN2836644.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            22223..24304
                     /locus_tag="ACG9BB_000674"
     CDS             22223..24304
                     /locus_tag="ACG9BB_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFN2836645.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            24453..28373
                     /locus_tag="ACG9BB_000675"
     CDS             24453..28373
                     /locus_tag="ACG9BB_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFN2836646.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            28374..29318
                     /locus_tag="ACG9BB_000676"
     CDS             28374..29318
                     /locus_tag="ACG9BB_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836647.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            29322..29525
                     /locus_tag="ACG9BB_000677"
     CDS             29322..29525
                     /locus_tag="ACG9BB_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFN2836648.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(29825..30061)
                     /locus_tag="ACG9BB_000678"
     CDS             complement(29825..30061)
                     /locus_tag="ACG9BB_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2836649.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(30244..30615)
                     /locus_tag="ACG9BB_000679"
     CDS             complement(30244..30615)
                     /locus_tag="ACG9BB_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFN2836650.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(30612..32171)
                     /locus_tag="ACG9BB_000680"
     CDS             complement(30612..32171)
                     /locus_tag="ACG9BB_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN2836651.1"
                     /translation="MIEQKMALLHTKNFSAYARKMLIDGYIVNMDTSDIRAQTAEIQK
                     IGVNVNQIAKRLNGMGPVYAQGHRGYKGGAGADMAVTKIHPIKSTLKKALDYIENPDK
                     TDGKLLVASFACSYETADIEFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGK
                     QLAEEVLQGKYPYVLTTHIDKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRL
                     CKENGLSVVKPGQSKGKSYAEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQA
                     QGYEIKPGKFISFRAPGQERFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLR
                     IDLENNIKAQQSAGYERWAKLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDE
                     AAATLKAMEHRLADMAVLIKNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAA
                     AKALKGQGVKKLPDLYALKAEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILR
                     MTPGKEWTQEL"
     gene            complement(32489..32859)
                     /locus_tag="ACG9BB_000681"
                     /pseudo
     CDS             complement(32489..32859)
                     /locus_tag="ACG9BB_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            33004..33189
                     /locus_tag="ACG9BB_000682"
     CDS             33004..33189
                     /locus_tag="ACG9BB_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFN2836652.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            33347..33514
                     /locus_tag="ACG9BB_000683"
     CDS             33347..33514
                     /locus_tag="ACG9BB_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836653.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            33746..33919
                     /locus_tag="ACG9BB_000684"
     CDS             33746..33919
                     /locus_tag="ACG9BB_000684"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836654.1"
                     /translation="MKSHTARCIRKKEAAHFAEAWGVPNKQNENAAGGQVVDQNIRKG
                     YSDPLLASIIGMF"
     gene            34132..34791
                     /gene="vanR-B"
                     /locus_tag="ACG9BB_000685"
     CDS             34132..34791
                     /gene="vanR-B"
                     /locus_tag="ACG9BB_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFN2836655.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            34794..36155
                     /gene="vanS-B"
                     /locus_tag="ACG9BB_000686"
     CDS             34794..36155
                     /gene="vanS-B"
                     /locus_tag="ACG9BB_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFN2836656.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(36246..37547)
                     /locus_tag="ACG9BB_000687"
     CDS             complement(36246..37547)
                     /locus_tag="ACG9BB_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN2836657.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            37826..38632
                     /locus_tag="ACG9BB_000688"
     CDS             37826..38632
                     /locus_tag="ACG9BB_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFN2836658.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            38650..39357
                     /locus_tag="ACG9BB_000689"
     CDS             38650..39357
                     /locus_tag="ACG9BB_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFN2836659.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            39354..40325
                     /locus_tag="ACG9BB_000690"
     CDS             39354..40325
                     /locus_tag="ACG9BB_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFN2836660.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            40318..41346
                     /gene="vanB"
                     /locus_tag="ACG9BB_000691"
     CDS             40318..41346
                     /gene="vanB"
                     /locus_tag="ACG9BB_000691"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFN2836661.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            41352..41960
                     /locus_tag="ACG9BB_000692"
     CDS             41352..41960
                     /locus_tag="ACG9BB_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFN2836662.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(42026..42199)
                     /locus_tag="ACG9BB_000693"
     CDS             complement(42026..42199)
                     /locus_tag="ACG9BB_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836663.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT        11988 a         9501 c        10525 g        10279 t
ORIGIN      
        1 tcctcgacag atgaatactt tgaaatatct ttataggcta agtccatttc gtctaatagc
       61 tgttttaaag aatcaatcat tctttctttt tcaccgtatt tgaaatcacc atttcttatc
      121 ttgtcttgaa gtttactgtt ggctcttttg aaaaatagct ctttagagat agagtgtctt
      181 tggttagcgt actcttcaat cagaggattg atattattag caagtatctc atttagataa
      241 atttcaaaat cttttgagta atattttgta aatatatttt ttattatagg attcaataac
      301 tcatagtctt tatctagcga aaacgtatca ctatatcttt cttgaatgtc tttaaagact
      361 ttgtgaggta tttctgggaa gtccttaata tatagctcat accctatatc gataaggtat
      421 gaagttatat attcttctaa agttccccat ttaatccagt caattgttga attagtgata
      481 gttgataata atttcagctt ttgtggattt ggtaaattgg tcccacgttc ccagttggat
      541 atggtcgcac gtggtgaatt atctattttc cttccaagtt cttccatagt aaaaccacat
      601 gaaaggcgta attgtttgat tcgttttcct gcttctgaag gatttggctt catctttttt
      661 ctccttttta tttttaaaga tagtatatca tatttataaa ataaaagtat ttaaaagtat
      721 ttacttttta tctggagtat gatactttat tcttataaat acaaataaat acattttttt
      781 acgcaatttg tatttatttg tacttataaa ggtagaggag ttatagaata attaattaaa
      841 aaggagaggt ttttgtggaa gcaataaaat taatgatttc agaagaacag gaggaaatgt
      901 ttctaaagca gttttatcaa attgctacaa aagctattga aaaggctcaa gaaaatgcgg
      961 ggatgacaag ggaatatctc aatcaaaaag aaatggcgga atacttaaat gtgagtgaaa
     1021 actttttaaa aaatgctgtg agaaatgagg gattgcccgt agttgaatta ggggcaagaa
     1081 aattttattc gaaaaaggca gttaatgagt ttatgtttgc tcgaatgaaa agaggggaaa
     1141 atattggaag atgatgaagt agaaattata aaaaaatgct ttaaaaaaag aaaaaaagtg
     1201 ttcccaaaag tttgcagact gggggaacac tcaataataa agtgctgaga aaactttatt
     1261 tatagctatt atatcaaaaa agaaggtgaa aaggtgtttt cttctggaaa tgttgtagtt
     1321 gacaaactta gagaaataaa ttttgacgga aatgtaatcc acacacatgg tacgatcaat
     1381 taagaaaaaa aacgaaaaaa ggtgttgaga agccctattt gaatgcaata gttatcttag
     1441 cagaaattac ctattggtat agacctaaaa aaatttttaa cgatgatggt cagctacttg
     1501 gttatcaaaa aaagtttctt gaagatattt tgcaaaaatc atataagcaa ttaagcaaaa
     1561 aaacaggatt atcagaacga gctataaaag atgcaattgt gtacttagag aaaaagggga
     1621 ttatcaaaag ggtttttaga aatatttatt atgagaaaag agcagttaca ttatcaaatg
     1681 ttatgtacat tgttttaaat gtacataaat tgatagaaat tacttttcct actttggatt
     1741 gcgaaaaaaa ggagtatatg ccgaaaattc gtcagagggg tatcgaaaaa aagagatatg
     1801 tctctttaaa aaaaggtaga ggtatgtaca aatttgtata tacgtataca aagactactt
     1861 cacagaacat atcaaagact actctcgtta tggaagaaga ggatattaat ataaagagga
     1921 tttacagtaa gattagtgtt ctttatgacg caaataacga agatggtcga aaattatttc
     1981 aagtgtatca ctatttgaga aacgctaatg tagatacaga agatatcaga aaaatcattg
     2041 aatatttatc tgaaaatact cagtatctta ttgctgagtg tattataaaa caacatgaag
     2101 catgcttttt tcaaagaaaa actgaagaag ggttatacga ttatgtaaca tattttttga
     2161 atggacttca gaaaagatat aaaagtcaat ttttagaaaa tgtagagcct atagatcagt
     2221 tcaaaaaagc tttcgaaatc aaagagttac ctgaaattac tatgcataat tggttgaaag
     2281 gagaagaatc atgattaaaa atcgcttaat agatcaaatg tggtgtcttg atcaaaatac
     2341 agatactctc gaattaatcg agagtatctg cttcaacaca gtagttctcg atttaagagc
     2401 tgaaaatgac aattgtgtaa ctcatatcat aattaatcaa aaaatggctc agcagctctc
     2461 tgagagccta agaaaatggg ctgaaggaga aaactatgaa agcaattaaa gaaaaaatcg
     2521 ttagtagaaa aataaactgg ttagcactca gtgtacttgt tttaatgatt agcgttgttt
     2581 ttacaagtaa tattgttgaa gctagcattt tcaattggtt tgatgacaca aaaagtcaaa
     2641 cagagttttt gaataattct ctttatagac cttacctaaa aaaatacaat ggagatttcc
     2701 ttagtcagtt cgggatttgg cttggatggg ctgtggtaaa aggcatgttt actgtgactg
     2761 attcgattca aaatatgatt ccagatgtgt tggatttgtt caatttcatt gaaagtacag
     2821 ggctaaataa tgtttatcaa tcagtcataa atacaattgt cgttggatta atgatccttt
     2881 cattgatgtt tgttggctac aagatgatta ctggaaaagg aacaatagat ttaagatctg
     2941 ttggaatgaa tatagtgatg tctgtagcgt taattctttt aatgccaaca atgatttcta
     3001 gcggtattca atttgccaaa atattttaca atgatgctac tacgattaca aattcagatg
     3061 acggagtcgc ttggtcattg ataaaacaag gagtaacaga tctggcatat attaataaaa
     3121 cagatcaata ctcaagcatt gataaaacgg aggatcgaaa taagttaaca cgaaaaaatt
     3181 ttcaacagac agatttaaca caagtgttga cggataaagt gattgataaa ttggaaaaag
     3241 aaaatccagc tgcggataat cttcgctatg aattagttga aaattctaat aatgaatttg
     3301 ttgccacaaa gttttctgat aacttcttat caacattttc tgatagcttg aaatcaggtt
     3361 attatcgata tcaagcaaat ctttggggga tttcgattgg gttgacagcg ttggcaatcg
     3421 cctatgtgtt cagcgcgttt gtgatcatta cagcaatatt agaacttgca tttaaacgtg
     3481 tgttaggtgt tcttgttttt gcgacagata ttgaaacagg acaacgttca aaagttgttt
     3541 tatccgatat tttacaatgt tacttaacag ttggatttca agggtttgga ctatctatgt
     3601 ttgctatgtt tatcaatttt ttaaatgctg gacaaggtat ctccacaaat atttttataa
     3661 aaacaattgc ttatatctgt gctgtgtttg tgctgataaa aggcagtgga acagtcatga
     3721 gatactttgg tgttgatatt ggactcaaag aaggctatgg gcaacttgct tctgcttttg
     3781 gtatgggcgc aatgctgttt agaaaaggat caactggatt taatcgtgct aaaggaagcg
     3841 gaaatggaaa tggatcagac agtggagaag gagaagatcg aaaaccagaa aagaatttcg
     3901 gcgaaactct atcaaaaaaa gctggcaaaa ctggaagagc tttgggatat gcccatgaac
     3961 gtggattatc aggtttagct tcagatggag ctacaatggc tagtgaaaga gcaataaaac
     4021 catttaagag tatgcgtgat atggctaatg ataccaagaa taagttcaaa gaaggtttag
     4081 atgatggtac agtgtccgct attaataaaa atagcaaacc tatgttagct aaaaataaag
     4141 aagatgaaac tggaaaatat gctgattcca tgccaacacg tctttcggat agaaaagatg
     4201 gtgctaaggt tgaaaatgcc gatagaatta tgtctagttc tgaacgtatg cgtgaagcaa
     4261 tgaaaaataa tgctgaatcg aataataatc ctgtaagtgc aattcagcaa aaagttcaac
     4321 aagatattga agaacgtaaa aatgctatgc acggtcaagc taagtctgct gaagaattaa
     4381 ttaatcagaa gcgacaagaa gctaaataca cgcctgaagc aatgaatcgt gaggaaatgt
     4441 taaggaaacg tgtagaaggg cgtacaggag ctaatacgga cgaaaaagaa gcgttgacca
     4501 aagaaagaat ccaagaagct aaaaactcaa ataccggatt agaaaaattg gtcaaagaaa
     4561 atctacaaaa ttctacaagt agtcaaggtg gccgatcagt agatgtccga gaaaatattc
     4621 aaggttcatt gaatggtcat gtcggacgga atgttgatgt aagagagaat ttacaaagct
     4681 cttcaaatgg tcaaggtact aagacaattg atgttcgaga aaatgtacaa aattctacaa
     4741 gtagtcaagg gtttaaatca atggatgtaa gagagaatgt acaaaattct acaagtagtc
     4801 aagggtttaa atcaatggat gtaagagaga atgtacaaag ttctacaagt agtcaaggat
     4861 tcaaaacagt agatgttcgg gagaacgttc aaaaagatag tggagaaatg aaagaaaaaa
     4921 cacaaaaaat caatatcgtt gaagacagaa agtcagcgaa aaaatttgat tcaaatcatg
     4981 aaacagtgat tattgatagt gaaatccgtg aaaatgataa gggatcaaaa ccaagacgaa
     5041 gatttaccta tgaagacaac gagttattca gagatacact taatgatcct aatccgcttt
     5101 tcgataatct attaaaaaaa taaaaggggg aattgtcatg aaattaattc taaagattag
     5161 tacgcatgat ttcttagaac ttgacgaatt atgtgaatca gtaaagaacg caacaaatga
     5221 aaaaatcacg gtcactaatt atattgaact gatgattcaa gaagaattaa agatgaaaca
     5281 tgagataaaa aacaacttga aatattgtta tctagaaaaa agtgaagaga taatccatga
     5341 attaaatggc ttttaaaaat tgtatttgac atgaatttac gacaaggaat tagtggggat
     5401 taagcgatta ttctcttcca ctttagagaa ataatgggtt aaaaagccag aatacgtaga
     5461 gcaatattga caacctataa aggagctatt gttgaaaatt gaagtaacaa aaagataaag
     5521 ggaggtttac atctatcaga ttaaaagtga tagtacaccc atgaaacctg caaaagagtt
     5581 gaacctaatg tatatcggtt taactctttt tatttgcaaa ttaaaaaaat atcaccgaag
     5641 tgacgaatga aactatgacc aaattaagca agaaaatata tcttgcaaat aaagtagtat
     5701 aaaaagatat tgataaagcc aatcaaaact ttgaatttca aacgttagat aatcaaacag
     5761 atacaaaaaa tgaaatcagt tctacgcgta cgattattaa aaataataaa ttttcactag
     5821 aagaaaatgg aaataaaatc aaataattag gaagaaaaaa caaagcaatt agaaaagcct
     5881 gcaagcaaat ttgagtaatc gagatccaat ttttttaagg aagtttaaca aaaatatgta
     5941 attctattat cactattaaa gagaaagttg gagcggaata taaagaaaag agaaaagtaa
     6001 ttaaacgcaa aaaaatgtag aacttaaaaa atataaagag gaacaaccgt taaaattagg
     6061 aatgatgttg tgtaaacatt ataattgcta tgatataaat gaagtgttaa agaaagcaaa
     6121 tttaaaccaa ttttgtgaac agaaaagcct tagtcactat gaccaaaact acggacgaaa
     6181 ctatgaccaa aagtaggtac taaattatga ccgaaaatag gtccaaaatg gattttttta
     6241 tgatatcaaa aattatgaaa tgattttgaa agaaaaccag ttggcacagc caacttagcc
     6301 accttcctgt aggaaggtgc tatcagtttt cccttatgct cttttctttc ttttttgttt
     6361 catatgtcaa atgccatttt gaaatgtacc atcttaagaa atttatcctt aaaaacaatt
     6421 gcgttattgg ataaaaagat gctacattat cttaaaaaag gaatcaaata tgatgaattt
     6481 agtggctagc atatcgtgtg attaagatac tttatagtgt aagttgaagg agaaaaaata
     6541 tttaaaatat acttcccatt tcagtgggaa gtaaaattaa tcttgttgtt tctactagta
     6601 atgtatcttt ttggaaacta tgtaacgatg taaacaagat ttttttcaga agaaaaaacg
     6661 ataaaagttg tgataaacga tttaaaaaat aggagaaaat atatcaagcg catggtatga
     6721 aacgcacatt taaaaagatt cgactaacta aaaaaataag tttacaaagc attgttgtgt
     6781 attacaagtg atctatacac tgaaaaatga tagagaaaat ctgatacgtc cgcaacaatg
     6841 gacaattgaa aatgaaagtg aggaaattgg aagaatggct gatgaaaata aagttgttcc
     6901 gcttgaaaca ctagtgataa ataaggaaga gtttgagcaa ctttttcttg atttttttgt
     6961 tcaatatgac aggatttatg attttagttc gtcactagat ttatttgaat tagaggcaga
     7021 aataatagaa aacagtcgag aggagaatgg taaaatcatc atttcagtag aaaaattcaa
     7081 tgaattattc tcagaaacat ttgaaataga tacagattat tatcaaccac aagaagtttt
     7141 ccaatcattt agaaatcaga ttattcgctc aggtaagacc gtaaactgaa aaacaataaa
     7201 agaaacgttg aaagaataat tattccttca acgtttcttt tttagaatgt tttaatgacc
     7261 aaagcagttt cattgtcagg cttaggtaat tcatgaaatc ccgtatttat agtgctcaat
     7321 tgatcaacta accatgtttt actttttgaa ttgtagataa atctaaaatt aatatcgtta
     7381 ttatcgtgtt gaaggggcgt ctccaaataa tctgctgtat attcatagtc aacgtaacct
     7441 gctaatacaa aagagagtat ccctttattt aatgttagat ttaatggata atcatacgac
     7501 tttatttttt tcaacttcat attgtacata tcttcagcca taaaagttga gttcgtgatt
     7561 tcatcttggg tctcattaaa aaagttatcc gaataatcgc ttatttttga aaagtcttta
     7621 ttggcaccag cctcaaaaaa ttgttcaact ttttcagtat aaagttgtgc taacgctcct
     7681 ttttcaatat taactttaat attttcatgt ctatcaattt gtgtgctgtt cagtaaggga
     7741 agatcagctt ttagttttat atcgtcttga tttctttcaa tttcaattgg caaaatcttt
     7801 tcagtagatt cagtttctga taaaggaaca tctagttttt tatcatgata ataagcttca
     7861 aatccatctg gaaccggaaa aaaatgtatt gggtaattct tttctgaggt tgttgtacta
     7921 gtttccgtac ttgtagaact tgtgtcagaa gtggtacacc cgcttatagt tccgatgagt
     7981 aaagcgcaag ttagaatgat tgcaaatttc ttcaaaaaaa ttcctcctta aaattgaata
     8041 tggactaata ctagtcccat tgtagcatac cgaatcttta agattaataa gaaaaaggac
     8101 taattttagt ccaaaaggtg tgctattctt gaaagaggaa aaaattattg ataaagaaaa
     8161 tgtaatcggt agcaatatta gacgagtccg attagaaaaa ggaattggtc aaaccgagct
     8221 gattagagac ctacagcttc gtaaaattgc tataactagg gaaacgttgg ttaagattga
     8281 aggtggccgc caacacatta agttggatca attgaaagcc atcaaagata ttctacaggt
     8341 gtcttatgaa gatttgcttc aatgactact tgtagtcatt tcgtttgaaa ttattatgtt
     8401 acacccttat gtgtcacgaa gaaatactgg tataataatg aatgggatat gtaacgatag
     8461 ggtatactgt tatgttgcct aataaattgt ggatcaaaat acaagaatgg agggatatcc
     8521 ttgtataagg catatgaatt ggattttaca aatgctgact taaatgcatt ctcctcaagc
     8581 tcgcaatcat atacacatgt agtaaatcaa atagatcaca accaaaaatc tattaacaag
     8641 agaatagaaa atctatttga aagtgaaaat gatttagttg attattattc taaggacagc
     8701 aaaattctag atgcagacga aattattaaa gattggtttc caactataaa agcagatatt
     8761 tttatatctc actctcatgc tgatgaaaaa ttagctatac gttttgcatc atggctattt
     8821 gaaaattttg gtcttactgc atttattgat tcttcggttt ggggatacag ttctgattta
     8881 ttaaaaaaaa tagaccaaaa atattgctat aaggaacaaa caaaaacata tgattatgat
     8941 aaaagaaatg taacaactag tcatgttcat atgatgcttt ctacagcttt aaataatatg
     9001 attgatagta cagaatgtct gttttttctt aatacaccta attcaattag tttgagcaat
     9061 gaaatcacta atgaacagaa atttacgtat tcgccatggc tatatagcga actgacgaca
     9121 gcttcaatag tggaaaaaaa gaatccacga ttggaaagta atcctcaaat gagcaccgag
     9181 gatgttagaa gcattataaa gcactattct tcgcaagaaa atctgcaaat aaaatattct
     9241 ttaaatttaa gtgaattaac ttatttacga aaagctgatt tgaaaaaatg ggctttagat
     9301 gctgtggaat gcactaaagg aagtaataca ttagatattt tatataaaaa caccattttc
     9361 actaatctct aatcaattta ctgaatcgaa aggaaccggg tagaatggat aagaaagttt
     9421 taataaagag gttctctgaa gctattgaaa aaggaaatgc agcaatattt gcaggtgcag
     9481 gattatcgat gtcacaagga tatgtaagtt ggccggaact acttaatgat cctgcaacag
     9541 aaatcgggtt ggattctaaa aaagaaacag acttagttac tctggctcaa tattataaaa
     9601 atgaaaatgg tggatcaaga ggaattttga atcagatact tatggataat tttggtgaag
     9661 aattagaaat atctgaaaat catcgaatac tagcaagttt acctattgaa acttattgga
     9721 caactaatta tgatcatcta atagaaaaat caatccgaga agcttataaa aaccctcagg
     9781 ttaagaaaaa ttatactcag ttagcaacaa ctaatccaaa tgtggataca attgtttata
     9841 aaatgcatgg tgatatagat gatgtcagtt cgacagtaat aacaagagat gattatgaaa
     9901 aatatgatga ttcgtacgcg ttatttaaag aaactttaaa aggagatttg ttaacaaaaa
     9961 cttttctctt tttaggattt agttttacag accctaattt agaaagaatt ttatcagata
    10021 taagatgggt tttgagagaa aaccaaagac ctcattattg tataatgaga aaaatattat
    10081 agaaaaaatt ttaggttatc gctgaaagcc ccggaaatac gggcatttcg caggatatga
    10141 gaactcaatt tactaccaat ttactacttt cggattgagc cgcatatatg ctgaaccata
    10201 ctgctaatac ccgccacccc aaatacggca catcataaat tgaatacgac accgtaaacg
    10261 cggcgttcct ctttcccgtc atgggagaac aggacgccgc ttttttatgc ccaaatagag
    10321 aaaggaggcg acgccatgct atttacttcc ggccctgacc gggcgtttga acggctgatg
    10381 caggaaaagc ccggtttcga tcactacgac ggcggcgggg cggacgcgcc ggaggattgc
    10441 ggcacctgcc gattttaccg cccggattgg aaatatcaat tctgccagtt tgcagagtgt
    10501 ccctatcagc cgggcaagct gaccgactat gcagcggtca aattcacagt gaaaggagcg
    10561 aacaacgcta tggcagtttt ccgtgtggaa aagaacaaag gctatacggt gatgtcaaat
    10621 caccacctgc ggaataagaa cctgacctta aaggccaagg gcctgctgtc gcagatgttg
    10681 tccttgccgg aggattggga ctatacccta aaaggactgt cctatatcaa ccgggaaagc
    10741 atcgacgcga tcaggacagc ggtatgggag cttgaaaaag ctggatatat ccggcgtgag
    10801 cagggccgtg acgcaaaagg caaaatggcc gatatggtct ataccatcta cgaacagccg
    10861 gtattggaaa acccggtatt ggaaaatcca acatcggata acccggtgtt ggaaaatccg
    10921 acatcggata atccggcgtc ggaaaatcca acgcaattaa ataaagaagt acaaagaact
    10981 gacttatcaa aaaaagaaaa aataattaca gatggacaaa gtacccattc cattcctatc
    11041 ccatccccga cccctgcccc tatggagggc gaggcagcgg ctacgccgcc ggaacggaaa
    11101 aggacaggaa aggatgaggc tgtacggatt tatcgggaaa tcattttgga gaacatcgag
    11161 tatgcctatc tcattcagga caggagcatt gaccgtgaac agcttgacga aatcgtagac
    11221 ctgatgctgg aaaccgtctg caccgcccga aagacaatcc gtgtcgccgg ggacgactac
    11281 cctgccgagc tggtgaaatc caagtacatg aagctgaccg gcgaacatat ccgctttgtc
    11341 cttgactgtc tgcgggagaa cacaacgaaa gtccgcaata tcaaacaata cctgcgggct
    11401 atgctgttca acgcccccag caccatttcc aactactaca cggcccttgt cgcgcacgac
    11461 atggcacagc ccgattgggg aaagcctaaa tccggcctgc cggactattc ctgcggcgag
    11521 ggcgaaagcc tgtaacgaga aaaggaggac gaccctatga agcagggcgc attgattttt
    11581 gacgagcgaa cagaccgtta cgacatccgc tttgatctgg ccgactatta cggcggcctg
    11641 cattgcgggg agtgtttcga cgtgatgatc ggcggcaaat ggaggcccac ccgcattgaa
    11701 atggccgaaa actggtatct tgtcggcatc cgggccgacg acctttccgg cctgcgggtg
    11761 cggatttgac ctatatctac ctgcggcgtc tggtggttcc gccttagacg ccgcttttga
    11821 ctttctgaca aggagggatg cgattgcagg aagaagttac tcaaaagacc attgccctgt
    11881 ccatgaaaac gggcaagctc acggctcaag tgctgcaagc ggcgctgaaa aagtacctgc
    11941 aacaccgggc caagggcaaa aacacgctgc accacggcca gcagagccta aagcagctta
    12001 aaaagcatgg ggctgatctc tccaacattg agatcaccga ggccaacatt ggagcgttca
    12061 agccatgcgc caagaaatac ggcgtggatt ttaccttacg aaaagataag accacccagc
    12121 cgccccacta cgttgtgatt ttcaaggcca aggatgcaga taaccttgat ctggcgtttc
    12181 gggagtttac cgccaagacg ctttccaagg agcagcggcc ctctatccgc aaggttttgt
    12241 ccgctgtgaa gcaaaaggcg gcggcccaga ccaagcagcg ggccaaggag aaaatcaagg
    12301 aaagggggct ggaactgtga agcctgacgt aaagaaattg ctgatcctga acctcccgta
    12361 tctgattttt gtgtacctgt ttgcgaaatg cggggaggcg taccggctgg ccgctggtgc
    12421 tgacctgtcg gggaagctcc tgcattttgc ggatggtttt gcggcggctt ttgccaaccc
    12481 tctgccaagc ctgcacctgt ttgacctgtg tatcggtatc gccggtgccg tgatcgtgcg
    12541 gctggtggtg tactgcaaga gcaagaacgt gaagaaatac cgcaaaggca tggagtatgg
    12601 cagcgcccgc tgggggacgg ccaaagacat agcgccttac atcgacccca agtttgaaaa
    12661 taatatcctg ctcacccaaa cggaacggct taccatgacc ggcaggccca aagaccccaa
    12721 gacagcgcgg aataaaaacg tgctggtgat cggtggcagc ggttcaggga aaacgagatt
    12781 tttcgtgaaa cccaacctga tgcaatgctt tccgaccacg gactatccga cttcgtttgt
    12841 ggtgacagat ccgaaaggca ctttggttct ggaaaccggc aggatgttcc agcaggcggg
    12901 ctaccgcatt aaaatcctga ataccattaa cttttccaag tccatgaagt acaacccctt
    12961 tgtctacatc cattcggaaa aggacgtgct aaagctggta aataccctga tttccaacac
    13021 caagggcgag ggggaaaagt cagcggagga tttttgggtg aagtcggaac ggttgtttta
    13081 ttcggccctg atcggctata tctggtacga ggccccagcg gaggaaatga acttctcaac
    13141 gctgctggaa atgataaacg ccagtgaagc ccgcgaggac gacccggagt ttcaaagccc
    13201 cgtcgatctc atgtttgagc gtttggagca gaaagacccc gaccattttg cggtgcgcca
    13261 gtacaagaaa ttcctgctgt ccgcaggcaa gacccgttct tccatcctga tttcatgcgg
    13321 tgccagactt gcgccctttg acatccggga ggtacgcgag ctgatggagg acgacgaaat
    13381 ggagctggac accatcgggg acgaaaagac ggtgctgtat ctcattatga gcgatacgga
    13441 cacgaccttt aatttcattc tggctatgct ccaaagccag ctaatcaacc tgctttgtga
    13501 ccgggctgat gatgtgtacg gcgggcggct gcctgtccat gtacgcctga ttttggatga
    13561 atttgccaac attggccaaa tacccaattt cgataaactc attgccacca ttcgaagccg
    13621 tgaaatatcg gcctctatta ttttgcagag ccagtcgcag ctaaaagcga tttacaagga
    13681 cgccgccgag atcatttcgg ataactgcga tagtgtgctt ttccttagtg ggcgcggcaa
    13741 aaatgccaag gagatttccg atgcgctggg gaaagaaacg attgacagtt tcaacaccag
    13801 cgagaaccgg ggaagccaaa cgtcccacgg attgaactat caaaaattag gaaaggagtt
    13861 gatgtcccaa gacgaaattg ccgtgatgga cggcggcaag tgtatcttgc agctacgggg
    13921 tgtgcgcccg tttttatcgg agaaattcga tattaccaag catccccggt acaaatacct
    13981 tgccgacgcc gacaagaaga atacctttga cgtggacaga tttttgacta tcacgcgccg
    14041 gaaacggcag caagtggtaa cgcaggatga agtattcgac ctgtatgaaa tcgacctgtc
    14101 ggacgaggac gccgccgctg aataaacagc ggcttttttc attccaacaa caaaatcaaa
    14161 aaatcattat gagccggaaa ccggcagaaa gaggtaaatt tatggcattt tttaattccg
    14221 cagttactac tttgcagact atcgtgatcg gactgggcgg cgctctgtgc gtatggggcg
    14281 gcatcaatct gttggaggga tacgggcagg acaatccggg ggcaaaatcg cagggtgtca
    14341 agcagcttgt cgctggcggc ggcgtggccc tgatcggtgt cactctggtt cccctgctgt
    14401 ccgggctgct gggctgatcc tggcctttgt ccctgtccaa acccttaact aatcgcctcc
    14461 gcgtcctccc ggccagaccg ggagggcgcg ggaaaggagg tgctacccgt gattagtgaa
    14521 atcattgaag aatggataaa aggcatcctc atggacggta tcacgggcaa cctgtccggg
    14581 ctgttcgata cggtaaatgc caaagtcgga gaaatcgccg ccgacgtggg acgaaccccg
    14641 caggcatgga acagcggtat tttcaatatg ctgcacagcc tgtcggaaac cgtgatcgtc
    14701 cccatagccg gggccatact cgcccttgtc atgtgctatg agcttatcca gatgataacg
    14761 gaaaagaaca atatgcacga ctttgacagc tccatgttct tccgctggat attcaaatcc
    14821 gcgtttgcaa tcctgatcgt aacgaacaca tggaacatcg tcatgggcgt attcgacgcc
    14881 acccaaagcg tcgtcaatca aagcgccggt gtgattatcg ggcagacatc cattgatttt
    14941 aacaccctga taccagacct tgaaagccaa ttggaggcta tgagcgtggg cgctcttttg
    15001 gggctgtggt tccaaaccct tgttgtgggc ctcaccatga atatcctttc catctgcatt
    15061 ttccttgtga cctatggccg catggtggaa atctatgttg tcacgtcgct ggggcctatc
    15121 ccccttgcca caatgggcaa cgcggaatgg cgcggcatgg gccaagggta tctgaaatcc
    15181 ctgctggcgc tgggcttcca agccttttta atcatggttg tggtgggtat ctacgcggtg
    15241 ctgatacaga atatcggttc cgcttccgac atatccggcg caatctgggg ctgtatgggc
    15301 tatacggtgc tgctgtgctt ctgcctgttc aagaccggca gtatatcaaa ggccgtattc
    15361 ggggcgcatt gatcccaaaa tccaagcata cacaggagga acttgtttat ttcaacgtac
    15421 aacatcattt acgctgaccc gccgtggcgg tatgactgca aaaacgtaca gggcgcagcg
    15481 gaaaatcact attccaccat gagcattgac gagctgtgcg ccctgccggt ggaacggctg
    15541 gcgtccaaag actgcctgct gtttttgtgg gccacattcc ccatgctgcc ggaagctctg
    15601 cggttaatca aagcgtgggg cttttcattc aagaccgtgg cttttgtatg gctgaaacag
    15661 aaccgtaaaa gccttacatg gttttacggg ctgggccgct ggacaagggg aaacgcggaa
    15721 atctgcctgc tggcaacaca cggcaagccg aagcgtcgtt ctgcctctgt ccatcagttt
    15781 attatcagcc cgattgaaca acacagcaaa aagccggatg tgacccgtga aaaaatcgta
    15841 gagctggcgg gcgatttgcc ccgcgtggag ctgtttgcca gacagaaaac ccccggctgg
    15901 gacgtgtggg ggaacgaggt ggaatgtgac cttatcctga cgtgaaagga ggtgccaaat
    15961 ggcttatgta accgtcccca aggacttaac ccatgtgaaa agcaaggttg tgtttgggct
    16021 taccaaacgg caactgatct gtttttccgg ggcgctggtg atcggcgtcc ccttatattt
    16081 tctcacccgt gactatctta ccaacagcgc ggcggccctg ctcatggtat ttgccatgct
    16141 gccgggcttt ttgcttgcca tgtacgagcg tcacggccag ccccttgaag tggtgattat
    16201 gcagatcatc cagtgctgct tcatccagcc aaaggagcgt ccctatcaga ccgacaacac
    16261 ctacaccgcc cttgtgcggc aataccagat ggaacaggag gtaaaggcca ttgtccaaaa
    16321 aggaaatccc aagagaaacc gaaaaaacaa agctgacccg cgcccagcga aaagaaattg
    16381 acgcggtaat ccgtaaatat aagggcgacg gcaggcccca cacggcgcag cagagcatcc
    16441 cctacgaggt catgtacccg gacggcgtgt gccgggtatc gcccggcgtc ttttccaagt
    16501 gcattgagtt tgcggacatc agctatcagc ttgcccagcc ggacacgcag acggctatct
    16561 ttgaaaaact gtgcgacctg tataactatg tggacgcctc cattcatatc cagttttctt
    16621 tcctcaatcg gaaagttgac ccggtgcagt acgccaagag ctttgagatc gcgccgcagg
    16681 gggacgactt cgacgacatc cgggccgagt acaccggcat tctgcaaaag cagcttgcaa
    16741 acggcaacaa cggcatggta aagaccaaat acctgacatt caccatcgag gccgaaagcg
    16801 tgaaagcggc gcgagccaga ttgaagcgca tcggctttga cctgttaggc tatttcaaga
    16861 gcatgggggc cgtagcccat gtgatggacg gctgggaacg gctgaacctg ctgcatggcg
    16921 tgtaccaccc ggacggcgag atattcaatt ttgactggaa gtggctggcc ccgtccggcc
    16981 tctccaccaa ggattttatt gccccgtcct ccctgtgctt cggcaatgca aagacattcg
    17041 gcatgggcgg caaatacggc gcggtaagtt tcctgcaaat cctgtccccg gagctgtcgg
    17101 acgatatgct ggccgacttc ctcaatacgg aaagcggtgt tttagtcaat ctccatgtgc
    17161 aggccattga acagaccaag gcgatcaaga ccatcaagcg caaaattacc gaccttgacg
    17221 ctatgaagat tgcggagcag aaaaaggccg tccgcagcgg ttacgatatg gacattcttc
    17281 ccagcgacct tgccacctac ggcgaggacg ccaagaagct gctgacaaag ttgcagaccc
    17341 gcaatgaacg gctgttccag ttgacgttcc ttgtcctcaa cgtggccgac accaagcaaa
    17401 agctgaataa cgacgtgttc caagccgcag gcgtggccca aaagcataac tgtccccttg
    17461 tccggctgga ctaccagcag gaacagggcc ttgcaagcag cctgccgctg ggcgtcaatc
    17521 agatcaagat acagcgcagc cttacgactt ccagcgtggc cgtgttcgtc ccctttgtga
    17581 cgcaggagct ttttcagggc ggcgcggcca tgtattacgg catcaacgcc aagtcccgca
    17641 acatgattat gctggacagg aagcaggccc gttgccccaa tgccttgaag ctcggcaccc
    17701 ccggcagcgg caaatccatg agctgtaaat ccgagatcgt cagtgtgttc ttaacgacgc
    17761 ctgacgatat ttttatttca gacccggagg ccgaatatta cccccttgtc aaacggctcc
    17821 acggacaggt aatccggctg tcgcctacca gcaaggactt tgtgaacccg ctggacatca
    17881 acctgaatta ttcggaggac gataacccgc tggccctgaa atccgacttt gtgctgtcgt
    17941 tctgtgagct tgtcatgggc ggcaagaacg ggctggaagc cattgaaaag accgtgatag
    18001 accgggctgt gcgcgtgatc taccggccct atctggcaga cccccgcccg gagaatatgc
    18061 cgatcctgtc cgacctccac aaagcgttgt tagatcagca tgtcccggag gctgaccggg
    18121 tggcgcaggc ccttgacctg tatgtgtccg gctccctgaa cgtatttaac caccgtacca
    18181 acgtggacat cggcaaccgg cttgtgtcct ttgacattaa ggagctgggc aagcagctca
    18241 aaaagctggg gatgctcatt gtccaagacc agatatgggg gcgcgtcacg gccaaccgaa
    18301 gccaaggaaa ggccacatgg tattttgccg acgagttcca tttgcttttg aaagaggaac
    18361 agacggcggc ctattctgcg gagatttgga agcggtttag gaaatggggc ggcattccaa
    18421 caggtgccac ccagaacgtc aaagacctgc tttcctcccc ggaaattgaa aatatccttg
    18481 aaaacagcga cttcattacg ctgctcaatc aggcgtccgg ggatagaaaa atccttgcgg
    18541 aacgcttgaa tttgtccacc gaacagcaga agtacatcga caattccgaa cccggcgagg
    18601 gccttttgat ctttgaaaac gtggtgctgc cctttaccaa ccccatcccg cacaacaccc
    18661 agctttacaa gattatgacg acccgcttaa acgaggtggc gggagtatga cccggcgacg
    18721 ccacaaagga ggtgaaccac catgaaagag ctgaaagcca aggcgaaagt gacgcaggcc
    18781 atgacccgcg acgggctgac gctggaaaat caggccaccg gcgaggtgga gaacatttcc
    18841 agccgggagg ccgaacagga tttgtccgcg ccggggccgg gcggcaccgc cgataagctg
    18901 ctggaacggg ccgacgccat ccatgacggc cataaggcca agaaagccgc gaagcaggcc
    18961 ggggctgtgg ccgccgaggg ggacgctgcc ctgcaccggc catccgcccg gctccaactg
    19021 tcggaggaag aacgggccga cccggattta cagccctata tccgaaaatc ggacaaacgg
    19081 gccgataagc tggataaggc ccgcgccgcc atccccaaga agcgcgtccc ggtcaaggaa
    19141 accgtctttg acgaggcaag cggcaaagca aagaccgtcc tgcactttga caaacaggac
    19201 aaggggcctc cctccctgaa acccaatccg gcaggccgtc ctgtttccga ggtttttcta
    19261 ttcggccacg ggaaaatcca tgaagtcgag catgaaaacg tgggtgtgga gggcggccat
    19321 aagggggagg aacttgccga gcggcaggcc ggaaaagcca tccgcagcac catccggcac
    19381 cacaggctga aaccctactg ggacgcggaa aaggccgagc gcaggctatt gtctgccaac
    19441 gcggagtatt tctaccagaa atctttgcgg gataacccgg agatcgcaca ggcggccagc
    19501 aaccccattt cccggttctg gcagaaacag cagatcaagc gccagtacgc caaggccgcc
    19561 cggacagcgg gccagaccgc aggcgcggcg gcacagggcg cggccaagac agccggggcc
    19621 tccacgaaag cggccaaggc cgcagccaaa gaaagtgaac gggcggcctc ctttgtggcc
    19681 cgccactgga aaggggcgct ggccgtcctt gtgttggggc ttttgatgct gcttatcatc
    19741 ggcggcttac agtcctgcac cgccatgttc ggcagcgccg gaagcggcat cacggcgtcc
    19801 tcttactttt ccgaggatag cgatatgttg gccgccgagg acgcctacgc ggagctggaa
    19861 gccgagctgc aagactatct ggacaactac gaggccaccc atgactatga cgaataccat
    19921 tttgaccttg acgagatcag ccatgacccc tatgtgctga tttccattct gtcggcgctc
    19981 catgacggga tatttaccat cggggaggta cagggtgatt tgtccatgct ctttgacaag
    20041 caatatatcc tgacagaaaa cgtcgtggta gaaacccgtt accgcaccga aaccgataca
    20101 tggacggacg cggagggcaa cacccataca gacacctacc aagtgccgta tgactattac
    20161 atctgctacg tcacgctgga aaacttcgac ctgtcccatg tgccagtcta tatcatggac
    20221 gaggaaacgc taagtttcta ttccctgtat atgtccacgc taggaaaccg gcctgatctg
    20281 ttttccggca gccagtaccc caacgcctcc accctgacgc cgccgaccta ctacgacgta
    20341 cccccggagg ctttggagga tgccaccttt gccgccatgc tcaaagaggc ggaaaaatat
    20401 atcggttatc cctatgtgtg gggcggcagc tctcccagca ccagctttga ttgcagcggc
    20461 ttcatttcgt gggtaatcaa tcatagtgga tggaacgtgg gacggctggg ggcgcagggc
    20521 ctatataaca tctgcacccc tgtttccagt tcgcaggcca agcccggcga ccttgtattt
    20581 ttcaagggaa cctacgacac ccccggcgtg tcccacgtcg gcctgtatgt gggcaacaat
    20641 gtgatgctgc attgcgggaa cccgatttcc tacacgaacc ttacttcaaa ctactggcaa
    20701 tcccattttt acagttacgg gcgtttacct taacgccaga aaggagcttt caattatggc
    20761 aaccaaactt gaccgcatcg aaaaggacat ccagaaaacc aagtctaaga tcgcggagtt
    20821 tcagaaacag cttcgggagc tggaaacgca gaaaaccgaa caggagaatt tgcagatcat
    20881 ccagcttgtg cgcggcatga acatgaagcc ggaggaattt gccgcgttct tacgcagcgg
    20941 cgcaatgcag gccgcccctg ccgcaacccc ttaccatgaa caggaggaca atgcccatga
    21001 agaataagac cagacggatt tcccgtattc tccccgcact tctggccgtg atgctctgcg
    21061 tcacggcctt tcccgttacc gcccttgcag gcggcaatga cccggcaccg gccccgctgc
    21121 cggaggctac aaccgaaccc gctacgggcg gtttggagcc tgaaacagac gaaaccggca
    21181 ccgccacgga cgggacagaa accgaggccg aaaaagatac cggccctctg accggcaagg
    21241 acatcaccga cctgttttcc gcgctgttcg gttccaaggt gagcattgcc gccacggacg
    21301 acggcatcca gatcaccacc ggcaccgaca aaaaagaacc tgaacagacc ggcaccgtca
    21361 ccaccaacgg cggcaggctg aatgtccgca ccggcgcggg gcttgacaag accgcgttca
    21421 cccagcttcc caatgggacg acggttgagg tagtcggcac agacggcgac tggattaaaa
    21481 ttctcttgcc ggagcgcatc ggctatgtcc attccgacta tatgaccgtc agcgaaaagg
    21541 aagtagcggc taccggcgag ggcggttttt ccctctccat tgacccggag gaaattgctt
    21601 ctctgctgga actgttcaat ggcggcgggg ccagcgccgc cctgtccccg gacgggaacc
    21661 tgtcactgat tgacgacatc ggcagcgccg cgaaaaatgg caagcagttt atcaccgtgg
    21721 aaaccaaaaa cggcaatgtg ttttatctga ttattgaccg ggacgacgag ggaaaagaaa
    21781 ctgtccattt cttaaatcag gtggatgaag ccgatctgct gacgctgatg gaggacgggg
    21841 aaaccgtcac ccccgctgct gtctgctcct gtaccacgaa atgcaaggcc ggggctgtga
    21901 acacgaattg cccggtatgt aaaaccaacc tgaccgagtg cagcggcccg gagccgcagg
    21961 aaccccagcc ggaggaaccc gaagccccgg aggaagaacc caagggcggc gcgggcggcc
    22021 tgatcgtgtt cctgctggtg gcgctggcgg gcggcggtgc ggccctttac tatttcaagt
    22081 tcaaaaagcc caaggctgaa accaccggca gcgacgattt gggcgagtat gattttgggg
    22141 aggacgagga tttggaggac gaggaacccg aaaccgagtt tgacccggaa gctgattttg
    22201 tgagtgaaca ggaggacgag gaatgacata taaacttgta atcgcggaaa aacccagcgt
    22261 ggcccgcagc attgcgggcg tgatcggggc cacggagaaa cacgacggct atttggaggg
    22321 gaacggctat ctggtgagct ggtgcatcgg acatctggtg tcctttgccg acgccggacg
    22381 ctatgacgag cgtttcaaaa aatggcgcta tgaggatttg cccattctcc cggaaacatg
    22441 gcagtacatc gtccccgacg acaagaaaca gcagtttgac gttctctgtt cgctgatgca
    22501 gcgccccgac gtgaccggcc ttgtgtgcgc caccgacgcc gggcgcgagg gggaattgat
    22561 tttccgtttc gtctaccaga tggcgggatg ccaaaagccc tttgagcgcc tttggatttc
    22621 ctctatggag gacagtgcta ttaaggacgg ctttgcccac ctgaaaccgg gcggcgacta
    22681 tgatccgctg tatcaatcgg ccctgtgccg ggccaaggcc gactggctga tcggcattaa
    22741 cgccacccgg cttttttccg tcctgtacca caagaacctg actgtgggcc gcgtccagac
    22801 gccgacctta aaaatgctgg tggacaggga cgccaagatt accgatttca agaaagagaa
    22861 atatcacatc gtccatatca cggcgggcgg cgcggacgcg gtaagcagcc gcttttccga
    22921 cgctgccgag gcaaacaccg tcaaggcggc ttgtgcaaaa gcgcaggccg tttgcgtttc
    22981 cgtgacgcgg gagaaaaaga cggaacagcc gcccaagctc tatgacctta ccaccttgca
    23041 gcgggaggct aaccggcttt tcggctatac cgccaagcag acccttgact atgcccaggc
    23101 actgtatcta aagaagctgc tgacctatcc gagaacggat agtcaatatc ttaccgacga
    23161 tatgctgccc gccgtggaaa gccttgtgtc cggcctgtgg gagctggtgc cgtttgccaa
    23221 gggcctgaac ctttccccgc agtttgaccg catcctgaac agcaaaaagg tgtccgacca
    23281 ccacgccatt atccccaccg ctgaatttgt gaagcagggc tttggcactc tggccgagag
    23341 tgaaaaaaag ctgctgtccc tgatctgctg caagctctta tgtgccgtgg ccgggccgca
    23401 tatctatgag gccgtcaccg ccacatttac ctgtgccggg caggagttta ccgccaaggg
    23461 caagaccgtc ctttcccccg gctggaaaga gattgaacgc cggttccgtt cttccttaaa
    23521 gaccgacgcc gacgaggacg ccgaggcggt gcgggagctg ccggagcttt gcgagggcca
    23581 gacctttgca gacgtggccg cctctgtcac cgagcatttc accacgccgc ccaagcccta
    23641 caccgaagat acgcttttgt ccgctatgga acgggccggg gccgaggata tgccggagga
    23701 tgccgagcgc aagggattag gcacaccggc cacccgcgcc gccattctgg aaaagctggt
    23761 gcagatgggc tttgtccaga gaaaaggcaa gcagctcatt cccaccaagg acggcatcaa
    23821 tctggctgtg gtgctgccgg aggcgcttac ttctccccag cttactgccg aatgggaaaa
    23881 ccggctgacc gagatcgcca aggggcaggc cgatcctgac gaatttatgg acggcatcga
    23941 gacgcaggcg cgggagctgg tgcagaccta ttcctgtatc agtgaggaaa agcagaaatt
    24001 gtttcaggcc gagcgtgttc ctatcgggac gtgtccccgc tgtggtgagg ctgtctatga
    24061 gggcaagaaa aactactact gcggaaaccg ggcctgccag ttcgtcatgt ggaagaatga
    24121 ccgatttttt gaggaacgca agaaagcgtt caccccgaag atcgccgctg cgctgctcaa
    24181 aaccggcaaa gtcaaggtaa agggcctcta ttccgtaaag accggcaaaa cctacgacgg
    24241 gacggtgctt ttggctgata ccgggggcaa gtacgtcaac taccgcattg aacacaggag
    24301 ctaaaggaca gtaacacagc aagcataggg agtgtggcta cactcccggt aaaatccccc
    24361 ggccgcgtcc ctgccgggcc ggggattttt gcttgttggg gaagtcccca accccctatt
    24421 ttttgaaaca gaacagagaa aggagtgacc gcgtgaacga agtattttct atccaagtcc
    24481 ataaccggca gtttttggaa cagggcaaac aaggcgtctg gctggatttg cccaccacaa
    24541 ccgaaacgct gcaagccgcc ctgcgggatg ttggcattac tgccgacaat ccgcaggatt
    24601 tttttatcaa cgggtattcc tgcccggagg acagacacct tgccattccc tatgacatgg
    24661 tgctggccgc cgacgtggac gagctgaatt tccttgccgc ccggctgggc cagcttgacg
    24721 ccgccgagat cgcagagctg aacgccgccc tgcaaaatcc ccgtggcggc tttgagaata
    24781 tcggccagat catcgactac accgaaaatg tggattacta tgtgcatctt cccgacgtta
    24841 ggggggatgg acagttgggc gactactacc tgaaccgttc cggcatggtg gatatgccgg
    24901 aggaatggaa agccgggatt gatacggcgc agtttggacg ccacattgcc cagcaggaac
    24961 aaggcgtttt tacccagtac ggctatctgg tgagaagcgg cgacgaatgg cagcgcgtcc
    25021 atgagggcca gccggtgccg gaggaatacc gggtgatgca ctacccccag ccggaagctg
    25081 accgggagga accgcaggcc cagcagccgg aggcatccga aagccccgac aaagcgccgc
    25141 ccccggttat cccgatcatg ctggacggca tgaacagcaa agaacgcatg aaagtgatta
    25201 ctgaccgtct ggaaaccggc gtacaggagc tttttgaaag tagccgctat atggcttact
    25261 tatccgctat gtccaagatg cacgattaca gtctgaacaa tacgctgctg atcgccatgc
    25321 agggcggttc gattgtggcg ggctataaca ggtggcagga tgaatttgac cgccatgtga
    25381 agcggggcga gaaaggcatt aagatactgg ccccggcccc gttcaaggtt aagcagcaga
    25441 tggagaaaac cgacccggcc acccagcagc ccgtgatcgg cgcggacggc aagcccgtga
    25501 ccgaagaaca ggaagtgacg gttccggcct ttaaggtggt gacagtcttt gacatcagcc
    25561 agaccgaggg caaggatttt cccatgccct atatcagtga acttaccggc gacgtgcagc
    25621 agtatcagga tttctttgcc gcgctggaac ggacatcacc ctatcccata agcattgagc
    25681 cgatttcaga cggggccaag ggccgctgct tctacgcaaa acagcgcatt gccatcaacg
    25741 agggcatgag cgaattgcag accctaaaga ccgccatcca tgagattgcc catgccaagc
    25801 tccacgccat tgaccgggag gcccccgccg aggaacaggc ggcccggcca gaccgcagca
    25861 cccgcgaggt acaggccgaa agcgtggcct ataccgtctg ccagtattac gggctggaca
    25921 cgtcggacta ttcttttggc tatgtggccg attggagcag cggcagggag ttatccgagc
    25981 tgaaagcctc ccttgaaacc atccgggcca cggccagcga attgataacc gagatcgacg
    26041 gccattttgc ccagcttcag caggagcgcg aggcccggca ggaacaggag gccgtcacgg
    26101 ttgacgttac cccggaggca gcggccccgg cgcaggccga ggaagaacag gccccggccc
    26161 ttgatccggc agccgagccg cttgtgactg tgctatggag cgaaagcccc cagctaaagg
    26221 acggccagca aatgccgttg catgaggccg acgcgctgtt tgcccgtctg gatgcagcat
    26281 atccggcaga ggcaggctat gacaaaaccg cgttccgcat tgactgtatc tttcagggcg
    26341 agccggacag ctacgagggg cggcaggatt ttggagatag ggacggttcc ctgatcgaac
    26401 atatccagag ctaccaccaa ttttacgcgc aggacgaaaa ctggaaaaag catgtaatcg
    26461 ctacaaccgg catagagggc tgggagcagg acaaggccca tcgggatatg ctgttaggcg
    26521 agtttgtccc ctatatgaag ctgcattgca gccttgccca aatggaacag acggcgcagg
    26581 gccgcttaca gtccggggaa accctgacgc cggaggaaac ggcctatttg ggggcgcttc
    26641 tctcttatgt gcaggagtgc cgccccatgc tcaatcaggg ccagtatcag ttgccggagc
    26701 cgccgaaact ggctgacttc gaccagagct tgcaggacta caaagcacag gtacaggccg
    26761 agatcgcgca ggaggccgcc gcagccggta tgaccgtgga ggaatacgcg gcggctggct
    26821 atgaagcacc ccaacaggac accttttcca tctaccagct tcgaggcggg gaggaaaccc
    26881 gcgactatcg gtttgagcca tacgagcgtt tacaggccgc agggctggcc gtggacagtg
    26941 ccaactatga ccttgtttat acggcaccgc tggataaaag cacgtctttg gaggatattt
    27001 accgcacatt caacatcgac cacccggcag attttagggg acacagcctt tccgtttccg
    27061 acgtggtggt gctgcaccag aatggacagg acaccgccca ctatgtagac agcttcggct
    27121 atatggaagt gccggagttt ttgcgggaaa atcccctgcg taccgccgaa ctgtctacgg
    27181 agcagaacga aaacatgatt gacggcatta tcaacaatac gccgcccgaa ccgcagccgg
    27241 aggaacccga acagctatct tttgaggatg tggcagttgc ttccggccag acacaggagg
    27301 aacccccgga ggctgcgccc ccgcaaacgg ctgtccgcta ttatcccatc aatgagggag
    27361 ccgcccgccg cgccaaggaa gccatgagct tttctgacta ccggcccggc agcgccacgg
    27421 cggaataccg gcactatgtc gatcaggccg tggaaattgc ggagcgccag aaaaagcgtg
    27481 tggagccaga gtatcacgaa aagatagaca gcctccttga cacctacgcc cgcaagctgg
    27541 ccgagaacat gaataagggc tatgagatta ccgcccgcgt cccctctgtc ctgattgccg
    27601 ggccgtccaa tttcccggta cgggcgaaag aaaagcagaa cgccgccagt gaccgcaaca
    27661 tggaggaatt tcagtacatt cagggcttgc ttgacaaaat ccgcagcacc ggcatgggtg
    27721 gtatcagcgc cgacgatccg caggccgttt ccaagctgga aaagaaactg gaaaagctgg
    27781 aagcgtcgca ggagttgatg aaggccgtca atgcctacta ccgcaagcat gggacgctgg
    27841 acggatgccc ccatctgatc gaaagaggca ttgaaaacct gaaagcggat atggcgtcgg
    27901 gctggcacta tgagaaaaaa ccgttccagt cctggcagct ctccaacaat aacgccgaga
    27961 tacgccggtt aaagggccgc attgaggaat tgacccgcca aaaggaggcg gcctatgtgg
    28021 gatgggagtt tgacggcggc actgtggaga tcaaccgggt ggccaaccgc ctgcaaatct
    28081 tctttgaggg gaagccggat gctgctgtac gcgacgagct gaaaagcaac ggtttccgct
    28141 ggtcgcccaa ggccggggca tggcagcggc agttaaacga caccacaatc cgcgtcgctg
    28201 accgtatcaa gtgcatccag cccctttccg gcgaaaagcc cagcgcctta cagcttgcgg
    28261 cccgcaggga gaaagaaaag ccctctatcc gggcgcagct caacgcggcc aagaccgaac
    28321 aggccaagca gccaccggcg cgggaaaaat caaagggatt ggaggtagag taaatgcaga
    28381 agtttgaaaa tgtggacgtt ctggccgcgc tggaacagat catgcggcag aacaccgcgt
    28441 tttatcaaag tgactttggc attgacaaag acattatcag gcgggatgcc gccagcgatc
    28501 aggccgcaga caagaccctg ctctggatgt cccggccctc cgggacgtac tgtttccggg
    28561 aacgtgatgt tttcctgaaa gacacccgac agtacaacac atggaaattt tacggggagc
    28621 agacccgtga caagatactg gcctacgcgg tggagctgac cggcacacag ggcggcacta
    28681 tccggggcaa tctctatgag ctggactatc cgcagcattt ccggcacgtc attgaaaccg
    28741 cccagccggt gagtgtgaac cggctctttt atgagcatgg cacccgtgac atcccggaga
    28801 gccagcgttt tgacggaagc cctgaccggg tattgggaaa ctttttgtac tatgaggccc
    28861 agccccatga cccggctgtg ctgcaggagg cgctacggac ggagcagcac agccgggggc
    28921 gggccgtccc cggcgatttc aaagtccacg tcaccgcttt gcatgacagc cggattgaaa
    28981 ccgaggcccg gcgtattgtg gacagcctga aagatctgtc cgcgcctaac agccccaata
    29041 aaacgcattt catggtggag atctccccgc actttgcggc gattgcttcc accaaggata
    29101 atgaccggct ctttgccatg ctgccctata aatccctgtg ttttaccggg atgaaaggca
    29161 ggcatggcgt gtatgccgtg attaaccggg acgagcgccg ggatgtgagt attcgcaggc
    29221 cccggccctc tatccgcaag cagctatccg acagcaaaca ggccgccagc ccaaaaaagg
    29281 cggcggccag aaccaaaaag aatgaattgg aggtataagg aatgatgcac tttaccgtgg
    29341 aggaagaaaa cctgatctgt atgtaccaca atgcagaccg gcgcagaacg ataaacaggc
    29401 tcaccgccgt catgccggtt atggatgggg atatgcgggc gcaggcccag cagacccttt
    29461 ccaagctgga acgcatgacg gatgccgact atgacggcca gaagttccac ttcacagacg
    29521 aataacgaac agcagacgcc gggcgcggcg gccatgtggt cgcttgcgtc cggcgtcttt
    29581 tctttttgcc tattcctacg catttagaac ccggtattgt cggggatagg atgaagtatt
    29641 cgcttgcccc tgcaaacatg cgggagaagc cttgcagcgg gcggttttca atcccttaaa
    29701 tgcgtaggtt gcagctataa ttagacccct gaaacagata tagccgcgca gcgggtgtat
    29761 ctgtttcagg ggtgtgcagg tgatagccgc aaagcggcgt aagagggtgc agccctcttg
    29821 ccgtctatct ctgttccagc gggaagctcg gcaatgcttc cagcagtttc agggaaatgc
    29881 gctgccgtat atcttcgtca atgactttct tctttttgcc gtttgcctgt ctgatctcca
    29941 ccgtggaaag ctcgttgatg tagtcagcat aatgggccaa cactttttcc gtggcccacc
    30001 tctcaccggc aacggcggct ttgatcgttt catagtccag cagcttttct tgtccgtcca
    30061 tcctgatacc cccgtcagac tgattttttc tatgatacca tattcggaag catggaacca
    30121 gagaaaacgg cgcttttctg ctggtaaatt ctacgcattt agaacagcac ttcggcgggg
    30181 ataggataaa acacccacat accccgctga ccatgccgga aaagccttga aatataaggg
    30241 cttttacagc tctaagtgcg taggtttcca gtatcttttc cgctggcgct cggcctcttt
    30301 gcggcggcgc atccgtttgg agcaggccgc gcagtatttg gcccggttag acttcggcgt
    30361 gaacgtggcc ccgcactcac agcagcgttt cacttcgtcc cggtctttgg tgatctcggc
    30421 atacagggcc gcatccagcg ggaggacagc ggccttaaac cagcggcaca gaagcgaata
    30481 ggaaatgctc tgtacgcaaa tacattcttc gccgtcgtcc agcaacaggc agttgccgtc
    30541 acaatagttg cagcactcat gcgtcagctt ccgggccttg cgatattgag ggtaattcat
    30601 tcgtggaata ctcatagctc ttgcgtccat tcctttcccg gcgtcatacg caaaatgccg
    30661 tccacattct gcttgatgat gccgtattcc tgcatctgct ttttcgcttc gccgtacttc
    30721 tcatacagcc gttccttttc ctcggccaat cgcttgtact cggctttcag ggcgtacaga
    30781 tcggggagct ttttcacacc ctgtcccttt agggcctttg ccgcagcttc atacaaaatc
    30841 aacgtgcttt catgctcacg ccggaatttt gccttatccg ctgcattgcg gtattccata
    30901 gccacgggcc agagctgctt gtaggtagaa atattcttta tcagcaccgc catatccgca
    30961 agccggtgtt ccatagcttt gagggtagcg gcggcctcgt cgctggcggc ggtgatctcg
    31021 gccactttgc tttccaaatc cgggtaggtg tctatcccat gttcagtcag gaaattcaaa
    31081 gtctttgcgg cctgcttcaa attgtggagc ttcgcccacc tctcatagcc tgccgactgc
    31141 tgggccttaa tgttgttttc cagatcaatc cgcaaagaaa tccccttgcg ctctttgggc
    31201 gctctggtga taacgcggcc tttgatacgc tctttgatgg cttcctcggt gtaggcttcc
    31261 cccaatgtct tgcagcgggt aaagcgttcc tgtccgggag cgcggaaaga aataaacttt
    31321 cccggcttga tctcatagcc ctgcgcctgc atgagccgca ggaaatcatc aaaatctttt
    31381 gcctgcggga tagcggcgtc aatggctgct ttgagcttcg ctttccagct cgtccctttc
    31441 cgctgggcgt cccactcggc atagctcttg cccttactct ggccgggctt caccacggac
    31501 aatccgtttt ccttgcacag cctgtcgctg gtgcgccgga tataggcata gctctgtttg
    31561 ttggaaatgt acttgcgctg atttaccata tccacggcac agaaaataat gtgattgtgg
    31621 acgtggccct tatcaatatg ggtagtcagc acatagggat atttcccttg cagcacttcc
    31681 tcggcaagct gcttcccgat ctcatgggcc tgttccggcg tggcttcgcc gggggcaaag
    31741 gattgtatca ggtggtggcc cagattattg cccttttggt atgcctgcgc cagcagcatt
    31801 tcaaattcaa tgtccgctgt ttcataagag caggcaaagg aggccaccag cagcttgccg
    31861 tcagttttgt ccgggttctc gatgtagtca agggcttttt tcagcgttga cttgatagga
    31921 tgtatctttg taactgccat atctgcgcca gcgccccctt tatatcctcg atgtccttgt
    31981 gcgtacaccg gccccatacc gttcagcctc tttgcaatct gattgacgtt gacgccgatc
    32041 ttctgtatct cggcggtctg cgcccggatg tcgctggtgt ccatgttgac gatatagccg
    32101 tcaatgagca tcttccgggc gtaggctgaa aagttcttcg tatgcagcag cgccattttc
    32161 tgttcaatca tagcccgttc ctccggcgtc acagggacgc gcagcacgat ttttcgcttt
    32221 ctgtttgcca tgcttcaccg tcctttccat tcaggggttt ggggatttcc ccaacaagca
    32281 aaacggacgc ggcccggcag ggacggggca ggccgttttt ccgggagtgg ctacactccc
    32341 tatgcttgct acgttactgt ttctccacta acaagacgtt tccaaagccc caaactaccc
    32401 gcaaaatagc cctctggaaa gaaaaaagtg caaaaaaata gaagctacgc tgtgcagctc
    32461 ccgctaaaag taaaacagag gatgccagtt acacatcctc cgtttcattc ttcttcggct
    32521 tttcaattag tatggcgctg tcaatcgcgc cctcgatgat cggcaggtat tcttcggggc
    32581 agagtttcag cttgtggctg accctttgcc gcagttcgct ttcctcccgc atgatctccg
    32641 ggttaaagta gcgttccaca ggaagcccgc aggctttaat aagctgtatg acaaccggca
    32701 gactgggaat ggcccgtcgt tttcgatgtt ggcaagatag cgccagtcaa tatttatttt
    32761 ctctgccatt gctttgcgcg aaacgcgctt tgcttgccgg gccgctttca catccgcgcc
    32821 gaaagtttca aagccgggac aatcttcaat attcgccata tcgcacctcc cacttacatt
    32881 gtatatttca tactctgccc gtggaatgtt ggtatatgca cctttcttaa agtttatatt
    32941 tcacattttg tttcttttat tcttcgagca aaagaattat aatgtaccct gtggaggtgt
    33001 actatggact acatgacgtt gaaagaggca ggcgagaaat ggggtgtgcc cccacgatgg
    33061 ataaactact attgtgctgc gggacgcatc cccggcgctg taaagatggc gacgatctgg
    33121 ctcattccca aaactgcgga aaagccgatt gacaggcgta caaagaaagg aaaggcggtt
    33181 actgaatagt ataatgaaac cggcgcattt tggatatggt tttgaagaga gagaaagcat
    33241 ggtattttgt cacggaaaaa ctataagatg atagatggca ggctgctcca gaccgataaa
    33301 aagtattctc atctgaaaca gaaagaaaag atttacacat ggatgtatga agaagcgaaa
    33361 ctttatcatg acagaaatgg aaaatgcccg aaacaaagac gaggaagctg ctgctgtatc
    33421 agccgtatat ggtagaattg aaaaggccgg catctggatt ctttatggtg aagtctccag
    33481 acattggata actgctggtc taatgcgcct ttgagtatgg ggtgattgat tttaataccg
    33541 gggaggtacg gctttttgag gaaaatattc tgggatttac atttggcgat tattacattt
    33601 cctgtaactg caaggacacg gatgatgaag atatgaggaa tatagacttg ctgattttct
    33661 actgtcttga acggtattag gactgaatct gctgcgcgga gtgcggcaag agcgaccgga
    33721 gggagtacac acaggccctg cctgtgtgaa aagccatacc gcaaggtgca ttcgcaaaaa
    33781 agaggccgcg cattttgctg aggcttgggg tgtccccaac aagcaaaacg agaacgccgc
    33841 aggcgggcaa gtggtggatc agaatatccg taaaggatat tctgatccat tgcttgccag
    33901 tattatcggg atgttttgac aaccgctgta atcgaaacat cccaaacatt aagaaggtgt
    33961 taattattat tatcttgatt taattcgttt aaaccaataa tatagataca tacaccatga
    34021 aaagaagcga gtatcccaat gacggatgat taaaatccga aaagaaatcg ctttgaaact
    34081 acagggaaac tacagactgt tatgttatct tcttaaaatg gagggatttt tatgtcgata
    34141 cgaattctac ttgtcgagga tgatgatcat atctgtaata cagtaagggc gtttctggct
    34201 gaggcaggat atcaggtgga tgcctgcaca gatggaaatg aggcatacac caagttttac
    34261 gaaaacactt atcaactggt tattcttgat attatgctgc ccggtatgaa cgggcatgaa
    34321 cttttgcgtg aatttcgtgc gaaaaatgat actcccattc tgatgatgac agccctgtcg
    34381 gatgacgaaa accaaatccg ggcgtttgat gcagaggcag acgactatgt aacaaagcca
    34441 ttcaagatgc agattttact aaagcgggtg gaagccctgc tgcggcgcag cggtgcgctg
    34501 gcaaaggaaa tccgtgtcgg caggctgaca cttctgccgg aggatttcac ggtactttgt
    34561 gacggtacag agctgcccct gacacgaaaa gaatttgaaa tccttttgct gctggtgcag
    34621 aacaaaggca gaaccttaac gcatgaaatc attttgtcac gtatatgggg atatgacttt
    34681 gagggtgatg gcagcacagt ccacactcat atcaaaaatt tgcgggccaa gctgccggaa
    34741 aatatcatca aaaccattcg cggtgtagga taccgattgg aggaatcata ataatggaaa
    34801 gaaaagggat tttcattaag gttttttcct atacgatcat tgtcctgcta ctgcttgtcg
    34861 gtgtaacagc aacactgttt gcacagcaat ttgtgtctta tttcagagtg atggaattac
    34921 agcaaacagt aaaatcctat cagccattgg tggaactgat tcagaatagc gataggcttg
    34981 atattcaaga ggtggcaggg ctgtttcact acaataacca atcctttgag ttttatattg
    35041 aagataaaga gggaagcgta ctctatgcca caccaaatgc caatacatca aatagtttta
    35101 gacccaactt tctttatgtg gtacatagag atgataatat ttcgattgtt gctcaaagca
    35161 aggcaggtgt gggattgctt tatcaagggc tgacaattcg gggaattgtt atgattgcga
    35221 taatggttgt attcagcctt ttatgcgcgt atatctttgc gcggcaaatg acaacgccga
    35281 tcaaagcctt agcggacagt gcgaataaaa tggcaaacct gaaagatgta ccgccgccgc
    35341 tggagcgaaa ggatgagctt ggcgcactgg ctcatgatat gcattccatg tatgtcaggc
    35401 tgaaagaaac catcgcaagg ctggaggatg aaatcgcaag ggaacatgag ttggaggaaa
    35461 cacagcgata tttctttgcg gcagcttctc atgagctaaa aacgcccatc gcggctacaa
    35521 gcgttctgtt ggagggaatg cttgaaaata tcggtgacta caaagatcat tctaagtatc
    35581 tgcgcgaatg catcaaaatg atggataggc agggcaaaat catttccgaa atactggagc
    35641 ttgtcagcct gaatgatggg agaatcgtac ccatagctga accgttggac atagggcgca
    35701 cggttgccga gttgctgccc gattttcaaa ccttggcaga ggcaaacaac cagcggttcg
    35761 tcacagatat tccagccggg caaattgtcc tgtccgatcc gaggctgctc caaaaggcac
    35821 tatccaatgt catattgaat gcagttcaga acacgccgca gggaggcgag gtacggatat
    35881 ggagtgagcc tggtgctgaa aaatgccgcc tttttgtttt gaacatgggc gttcacattg
    35941 atgatactgc gcttccaagg ctgttcaccc cattctatcg cattgatcag gcgcgaagca
    36001 gaaaaagtgg gcgaagcaga aaaagtgggc gaagcggttt aggacttgcc atcgtacaaa
    36061 aaacgctgga tgccatgagc ctccagtatg cgctggaaaa cacctcggat ggcgttttgt
    36121 tctggctgga tttaccgctc acatcaacat tgtaaatatt taaaacttaa atgattttgg
    36181 gctctttgtc aaataggact gatagagtga gataagaaca tctaaggtag aattcctttt
    36241 ctactttaga tgttttttga ttcataggta atcagattat ttagtaagaa aattctggtc
    36301 ttcattttcc gaaagcttcg aaagccataa gcgactcgtt tcaatgcttt gatgtgggta
    36361 tgcagatttt ctattttggc atttgaataa ggcaattcga gggctaaacg aatgccggat
    36421 tcatagttta taaaagcttt ttttatagaa tgcagttctt ttggaacgga attgggcatc
    36481 gttcttacaa gtttaaagaa agtgtccgcc tctttgtcgt gaaacgcttg aagaatttct
    36541 tgatagtaaa aataggcgat tcgtaattcg tcagagatcg aaagcagacg atccaccact
    36601 tcactttcgg tgagatacgc ccaaccaaaa ttgcgccatt tggtccattt gtcataaaca
    36661 agcttgtcct gtcgtttgat cagaagccga gacaacgcct ttaattgtcg ataatatttg
    36721 gcttgtgttg gatcgtttcg atcaaagctt ttcatgattc gtacgcgaat atcctcaaat
    36781 ttacgaatga gatgtttgac aatgtgaaaa cgatccacaa cgagttttgc ctttggaaaa
    36841 cattccttta tcagagaggc ataagaaaca ttcatgtccg tcacgatgac ctttaccttc
    36901 aatcgtgcgc gtcgttcaaa ttgcataaaa taagaaacga gagccttttt cttccgtgaa
    36961 ggaagaatat ccagtaattt tccagtatcg ccatccatac aagaaaagga gtagggaccg
    37021 acttgatttt tcagtgaacg aaattcatcc attaataaac aacgcggtaa ccaatttctt
    37081 tttacttttg tcatggggat cagagaagcg agtgtccgag aaacggtcga ccaagaaacg
    37141 ccgcaatgct tagcaattgt cgtcattgct tgtttttcag ttagctcttc taaaatttta
    37201 tagcgcactt gtttagcgat aaagcagttt ctatcgacaa tgtaggtatt cgccgtaaaa
    37261 taggtgtggc agccacgaca gtggtagcgt tgtttcgcta aagcaagata agtcagttgg
    37321 ttcgcacatt tattaagttg aatgagcgat tttttctttc cgttcttaac gatcatgcct
    37381 tcatgattca tctgtttaca caaaggacag cgacgaattt ctttacttaa cgtcgcagaa
    37441 acaacaagac tcttgatatt cttaatgctc ttcgattcca catttgtgat gaaaatgttt
    37501 ttatctttga ttccaagtag tagtttgata tgatgaagat caggcataca gaacgctcct
    37561 ttgattgtgt tgtggtgatt caattctaac ggagcttctg tatgttttgt tttttcatgc
    37621 tcaaaaggga ccgatagaat gattttctat cagtcctaga aattatagag ccgattttga
    37681 ccgacaggta taaccctgcc ggtctttttt tgctgctaca ggaaaactac agactggcta
    37741 cagggaaagt acagatgcgc ttgccataat aacaatcgta ccagccacaa atcgtagttt
    37801 tattgcaaag gaggcattcg atcaaatgga aaaaagcaac tatcattcca atgcggatca
    37861 tcacaaacgg catatgaaac aatccgtgga aaagcgggct tttctatgtg cgttcattat
    37921 ttcgttcaca gtctgcacgc tgtttttggg gtggagactg gcttccgtat tggaggcaac
    37981 acagataccg cccatccctg caactcatac aggcagcagc actgacgtag tggagaattt
    38041 ggaggaaaac gctcttgcca ccgccaaaga acagggagat gaacaggaat ggagcctgat
    38101 tttagtgaac aggcagaacc ccatccccgc acagtacgat gtggagcttg agcaactatc
    38161 aaatggtgag cggatagata ttcggatttc tccctatctt caagatttgt ttgatgccgc
    38221 aagaactgat ggagtttacc cgattgtcgc atccggatac cgaacaacag aaaaacagca
    38281 agaaattatg gatgaaaaaa ttgccgaata taaggcgaaa ggctacacct ctgcacaggc
    38341 taaagcggaa gcagaaactt gggtggccgt gccgggaacg agcgagcatc agcttggtct
    38401 tgctgtggat atcaatgcgg acggaattca ttcaacaggc aacgaggttt atagatggct
    38461 ggatgaaaac agctatcgtt ttggttttat tcgtcgctac ccaccagaca agacagagat
    38521 aaccggtgtg agcaacgagc cgtggcatta ccgatatgtt ggcatcgaag ctgccacaga
    38581 gatgtacaac caaggggttt gccttgagga atatttaaaa ccagaaaaat gagaaaagga
    38641 tgttatgtta tggacagaaa aagattgaca cagcgtttcc cgttcctgct tccaatgaga
    38701 cgggcgcaga gaaaaatgtg cttttatgcg ggaatgagat ttgacggctg tcgctatgcg
    38761 cagacgatag gggaaaaatc gctttcccat ttgctctttg aaacggattg cgcattatac
    38821 aaccacaata ccggatttga catgatatac caggaaaaca aggtgttcaa cttaaagctg
    38881 gcagcaaaga ccttaaacgg cttattgata agaccggggg aaaccttttc tttctggttg
    38941 ttcctgcata cgccattgac aattatccag cgcagaggtc acgaagtaaa ggagtttcca
    39001 gagccaaaca gtgacgagat taaaggggtg gatgcaacca tttcagaggg ctggattgat
    39061 ttaaaagtgc gaaacgatac cgactgcacc taccaaatat gggtgacctt agatgatgag
    39121 aaaatcatcg gtcaggtgtt cgccgacaaa gagccccaag cattatacaa aattacaaat
    39181 ggcagtatcc agtatgtccg tgaaagtggc gggatttatg aatatgccca ggttaaacgg
    39241 atgcaagttg ccttaggtac cggggaaata atagattgca agctgcttta tacaaacaaa
    39301 tgcaaaatct gttatcccct cccggaaagt gtggatattc aggaggagaa ccaatgagaa
    39361 aaagtgtggg cattactgtt tttggatgcg aacaggatga ggcaaatgct ttccgcgctt
    39421 tatcgccgga ttttcatatt atccctacgc tgattagcga tgcgatatcg gcagacaacg
    39481 caaaattggc cgctggcaat caatgcgtta gcgtaagcca taagtccgag gtttccgagg
    39541 cgacaattct tgcgctgaga aaggtcgggg taaaatacat ttctacccgc agcatcggct
    39601 gcgatcacat tgatacgact gccgccgaga gaatgggaat ctcggttggc acggttgcgt
    39661 attcgccgga cagcgttgcg gattatgctt tgatgctgat gctgatggcc atacggggtg
    39721 caaaacccac catgcacgcc gtggcgcaac aagatttcag attggatcgt atccggggga
    39781 aagaactggg ggatatgact gtgggagtta ttggaaccgg ccatatcggg caagcggtcg
    39841 tcaaaaggct gcggggattt ggatgccatg tgctggccta tgataacagc cgaaaaatgg
    39901 atgcagatta tgtccagctt gatgagcttc taaaaaacag cgatattgtt acgctccatg
    39961 tgccgctttg tgcggatacc cgccatctga tcggtcagaa gcaaattgga gagatgaagc
    40021 aaggcgcatt tttaatcaac accgggcgcg gagcgcttgt cgataccggg gcgctggtgg
    40081 aggcgttgga aagcggaaag ctgggtggtg cggcactgga tgtgttggag ggcgaggatc
    40141 agtttgttta taccgattgc tcgcagaaag tgcttgacca tccctttttg tcgcagcttt
    40201 taaggatgcc gaatgtgatc atcacacccc atacggcgta ctacactgag cgtgtgctgc
    40261 aggatactac agaaaaaaca atcaggaatt gtctcaattt tgaaaggagt ttacagcatg
    40321 aatagaataa aagtcgcaat catcttcggc ggttgctcgg aggaacatga tgtgtcggta
    40381 aaagccgcaa tagaaattgc tgcgaacatt gatacggaaa aattcgatcc gcactacatc
    40441 ggaattacaa aaaacggtgt atggaagcta tgcaagaagc catgtacgga atgggaagcc
    40501 gacagtctcc ccgccatact ctccccggat aggaaaacgc atgggctgct tgtcatgaaa
    40561 gaaagcgaat acgaaacacg gcgtattgat gtggctttcc cggttttgca tggcaaatgc
    40621 ggggaggatg gtgcgataca ggggctgttt gtattgtctg gtatccccta tgtgggctgt
    40681 gatattcaaa gctccgcagc ttgcatggac aaatcactgg cctacattct tacaaaaaat
    40741 gcgggcatcg ccgttcccga atttcaaatg attgataaag gtgacaagcc ggaggcgggt
    40801 gcgcttacct accctgtctt tgtgaagccg gcacggtcag gttcgtcctt tggcgtaacc
    40861 aaagtaaacg gtacggaaga acttaacgct gcgatagaag cggcaggaca atatgatgga
    40921 aaaatcttaa ttgagcaagc gatttcgggc tgtgaggtcg ggtgtgcggt catggggaac
    40981 gaggatgatt tgattgtcgg cgaagtggat caaatccggc tgagccacgg tatcttccgc
    41041 atccatcagg aaaacgagcc ggaaaaaggc tcagaaaatg cgatgattac agttcccgca
    41101 gacattccgg tcgaggaacg aaatcgggtg caggaaacgg caaagaaagt atatcgggtg
    41161 cttggatgca gagggcttgc ccgtgttgat ctttttttgc aggaggatgg cggcatcgtt
    41221 ctaaatgagg tcaataccct gcccggtttt acatcgtaca gccgctaccc acgtatgatg
    41281 gccgccgcag gaatcacgct tcctgcactg attgacagcc tgattacatt ggcgttaaag
    41341 aggtgataag tatggaaaat ggttttttgt ttttagatga aatgttgcat ggcgtccgtt
    41401 gggatgccaa gtacgccaca tgggataact tcacaggaaa accggtggat gggtatgagg
    41461 tgaatcgcat catcggcaca aaggccgtgg cgtttgctct gcgcgaggca caaatccatg
    41521 cggctgcgct tggctatggc ttgcttttat gggatggata tcggccaaga actgcggtgg
    41581 actgcttcct gcgttgggca gcgcaaccgg aggacaatct cacaaaagaa aaattttacc
    41641 ccaatataga gcgagccgag ttgattacaa agggttatgt ggcctcacaa tccagccata
    41701 gccgtggaag cgcaattgat cttacgctct accacctgga tacaggggaa cttgtttcaa
    41761 tgggaagtaa cttcgatttt atggacgaac ggtcgcatca tacagcaaaa gggatagggg
    41821 atgcagaggc acaaaatcga agatgcttgc gtaaaatcat ggaaagcagc ggatttcagt
    41881 cttatcgctt tgaatggtgg cactataagt tgattgatga gccatacccc gatacctatt
    41941 ttaattttgc cgtttcataa tgaaagtatt ggttttgtat cagattttgg atttgataaa
    42001 atctatgctg gtcatggaca atcttttact ggaaagtggt taaagcatct tttgtaatac
    42061 attgcaaatt atagtattca atctgcccag cggcagaaaa gaaaaaaacc gctgttgggc
    42121 agacctttcc cacgcccatt ttgtattttg ctggcggcgg ttttgagaaa tcaaggccgc
    42181 cgttcttttc tggcagcaaa aggtgtggcg gcccatagga ggatgccgct atgctgtttt
    42241 gctattgcca tgctctaatc cacttccacc actcaaaaaa agccttgccc ctc
//
LOCUS       DAZAFF010000013        39834 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000013 DAZAFF010000000
VERSION     DAZAFF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.013"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..1162
                     /locus_tag="ACG9BB_000694"
     CDS             146..1162
                     /locus_tag="ACG9BB_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2836664.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            1303..2604
                     /locus_tag="ACG9BB_000695"
     CDS             1303..2604
                     /locus_tag="ACG9BB_000695"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFN2836665.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            2650..3312
                     /gene="deoC"
                     /locus_tag="ACG9BB_000696"
     CDS             2650..3312
                     /gene="deoC"
                     /locus_tag="ACG9BB_000696"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFN2836666.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            3333..3731
                     /locus_tag="ACG9BB_000697"
     CDS             3333..3731
                     /locus_tag="ACG9BB_000697"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFN2836667.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            3797..4879
                     /locus_tag="ACG9BB_000698"
     CDS             3797..4879
                     /locus_tag="ACG9BB_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN2836668.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            5091..6653
                     /locus_tag="ACG9BB_000699"
     CDS             5091..6653
                     /locus_tag="ACG9BB_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2836669.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            6646..7779
                     /locus_tag="ACG9BB_000700"
     CDS             6646..7779
                     /locus_tag="ACG9BB_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2836670.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            7779..8735
                     /locus_tag="ACG9BB_000701"
     CDS             7779..8735
                     /locus_tag="ACG9BB_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2836671.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            8834..10000
                     /gene="deoB"
                     /locus_tag="ACG9BB_000702"
     CDS             8834..10000
                     /gene="deoB"
                     /locus_tag="ACG9BB_000702"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFN2836672.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            10024..10842
                     /locus_tag="ACG9BB_000703"
     CDS             10024..10842
                     /locus_tag="ACG9BB_000703"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFN2836673.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            10859..11566
                     /gene="deoD"
                     /locus_tag="ACG9BB_000704"
     CDS             10859..11566
                     /gene="deoD"
                     /locus_tag="ACG9BB_000704"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFN2836674.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(11864..12550)
                     /gene="gpmA"
                     /locus_tag="ACG9BB_000705"
     CDS             complement(11864..12550)
                     /gene="gpmA"
                     /locus_tag="ACG9BB_000705"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFN2836675.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(12722..13303)
                     /locus_tag="ACG9BB_000706"
     CDS             complement(12722..13303)
                     /locus_tag="ACG9BB_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2836676.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(13436..14119)
                     /gene="rpiA"
                     /locus_tag="ACG9BB_000707"
     CDS             complement(13436..14119)
                     /gene="rpiA"
                     /locus_tag="ACG9BB_000707"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFN2836677.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            14438..14851
                     /gene="rpsL"
                     /locus_tag="ACG9BB_000708"
     CDS             14438..14851
                     /gene="rpsL"
                     /locus_tag="ACG9BB_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFN2836678.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            14935..15405
                     /gene="rpsG"
                     /locus_tag="ACG9BB_000709"
     CDS             14935..15405
                     /gene="rpsG"
                     /locus_tag="ACG9BB_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFN2836679.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            15499..17586
                     /gene="fusA"
                     /locus_tag="ACG9BB_000710"
     CDS             15499..17586
                     /gene="fusA"
                     /locus_tag="ACG9BB_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFN2836680.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            17758..18945
                     /gene="tuf"
                     /locus_tag="ACG9BB_000711"
     CDS             17758..18945
                     /gene="tuf"
                     /locus_tag="ACG9BB_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFN2836681.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            19352..19660
                     /gene="rpsJ"
                     /locus_tag="ACG9BB_000712"
     CDS             19352..19660
                     /gene="rpsJ"
                     /locus_tag="ACG9BB_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFN2836682.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            19691..20320
                     /gene="rplC"
                     /locus_tag="ACG9BB_000713"
     CDS             19691..20320
                     /gene="rplC"
                     /locus_tag="ACG9BB_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFN2836683.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            20348..20971
                     /gene="rplD"
                     /locus_tag="ACG9BB_000714"
     CDS             20348..20971
                     /gene="rplD"
                     /locus_tag="ACG9BB_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFN2836684.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            20971..21261
                     /gene="rplW"
                     /locus_tag="ACG9BB_000715"
     CDS             20971..21261
                     /gene="rplW"
                     /locus_tag="ACG9BB_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFN2836685.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            21300..22133
                     /gene="rplB"
                     /locus_tag="ACG9BB_000716"
     CDS             21300..22133
                     /gene="rplB"
                     /locus_tag="ACG9BB_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFN2836686.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            22176..22454
                     /gene="rpsS"
                     /locus_tag="ACG9BB_000717"
     CDS             22176..22454
                     /gene="rpsS"
                     /locus_tag="ACG9BB_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFN2836687.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            22477..22824
                     /gene="rplV"
                     /locus_tag="ACG9BB_000718"
     CDS             22477..22824
                     /gene="rplV"
                     /locus_tag="ACG9BB_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFN2836688.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            22838..23494
                     /gene="rpsC"
                     /locus_tag="ACG9BB_000719"
     CDS             22838..23494
                     /gene="rpsC"
                     /locus_tag="ACG9BB_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFN2836689.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            23497..23931
                     /gene="rplP"
                     /locus_tag="ACG9BB_000720"
     CDS             23497..23931
                     /gene="rplP"
                     /locus_tag="ACG9BB_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFN2836690.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            23921..24109
                     /gene="rpmC"
                     /locus_tag="ACG9BB_000721"
     CDS             23921..24109
                     /gene="rpmC"
                     /locus_tag="ACG9BB_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFN2836691.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            24134..24400
                     /gene="rpsQ"
                     /locus_tag="ACG9BB_000722"
     CDS             24134..24400
                     /gene="rpsQ"
                     /locus_tag="ACG9BB_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFN2836692.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            24458..24826
                     /gene="rplN"
                     /locus_tag="ACG9BB_000723"
     CDS             24458..24826
                     /gene="rplN"
                     /locus_tag="ACG9BB_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFN2836693.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            24863..25171
                     /gene="rplX"
                     /locus_tag="ACG9BB_000724"
     CDS             24863..25171
                     /gene="rplX"
                     /locus_tag="ACG9BB_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFN2836694.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            25199..25738
                     /gene="rplE"
                     /locus_tag="ACG9BB_000725"
     CDS             25199..25738
                     /gene="rplE"
                     /locus_tag="ACG9BB_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFN2836695.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            25757..25942
                     /locus_tag="ACG9BB_000726"
     CDS             25757..25942
                     /locus_tag="ACG9BB_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFN2836696.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            25978..26376
                     /gene="rpsH"
                     /locus_tag="ACG9BB_000727"
     CDS             25978..26376
                     /gene="rpsH"
                     /locus_tag="ACG9BB_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFN2836697.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            26408..26944
                     /gene="rplF"
                     /locus_tag="ACG9BB_000728"
     CDS             26408..26944
                     /gene="rplF"
                     /locus_tag="ACG9BB_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFN2836698.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            27105..27461
                     /gene="rplR"
                     /locus_tag="ACG9BB_000729"
     CDS             27105..27461
                     /gene="rplR"
                     /locus_tag="ACG9BB_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFN2836699.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            27482..27982
                     /gene="rpsE"
                     /locus_tag="ACG9BB_000730"
     CDS             27482..27982
                     /gene="rpsE"
                     /locus_tag="ACG9BB_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFN2836700.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            27997..28176
                     /gene="rpmD"
                     /locus_tag="ACG9BB_000731"
     CDS             27997..28176
                     /gene="rpmD"
                     /locus_tag="ACG9BB_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFN2836701.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            28220..28660
                     /gene="rplO"
                     /locus_tag="ACG9BB_000732"
     CDS             28220..28660
                     /gene="rplO"
                     /locus_tag="ACG9BB_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFN2836702.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            28660..29955
                     /gene="secY"
                     /locus_tag="ACG9BB_000733"
     CDS             28660..29955
                     /gene="secY"
                     /locus_tag="ACG9BB_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFN2836703.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            30017..30664
                     /locus_tag="ACG9BB_000734"
     CDS             30017..30664
                     /locus_tag="ACG9BB_000734"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFN2836704.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            30855..31073
                     /gene="infA"
                     /locus_tag="ACG9BB_000735"
     CDS             30855..31073
                     /gene="infA"
                     /locus_tag="ACG9BB_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFN2836705.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            31106..31222
                     /gene="rpmJ"
                     /locus_tag="ACG9BB_000736"
     CDS             31106..31222
                     /gene="rpmJ"
                     /locus_tag="ACG9BB_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFN2836706.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            31240..31605
                     /gene="rpsM"
                     /locus_tag="ACG9BB_000737"
     CDS             31240..31605
                     /gene="rpsM"
                     /locus_tag="ACG9BB_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFN2836707.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            31633..32022
                     /gene="rpsK"
                     /locus_tag="ACG9BB_000738"
     CDS             31633..32022
                     /gene="rpsK"
                     /locus_tag="ACG9BB_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFN2836708.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            32102..33040
                     /locus_tag="ACG9BB_000739"
     CDS             32102..33040
                     /locus_tag="ACG9BB_000739"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFN2836709.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            33068..33448
                     /gene="rplQ"
                     /locus_tag="ACG9BB_000740"
     CDS             33068..33448
                     /gene="rplQ"
                     /locus_tag="ACG9BB_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFN2836710.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            33720..34559
                     /locus_tag="ACG9BB_000741"
     CDS             33720..34559
                     /locus_tag="ACG9BB_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN2836711.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            34535..35404
                     /locus_tag="ACG9BB_000742"
     CDS             34535..35404
                     /locus_tag="ACG9BB_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN2836712.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            35394..36191
                     /locus_tag="ACG9BB_000743"
     CDS             35394..36191
                     /locus_tag="ACG9BB_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFN2836713.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            36394..37140
                     /gene="truA"
                     /locus_tag="ACG9BB_000744"
     CDS             36394..37140
                     /gene="truA"
                     /locus_tag="ACG9BB_000744"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFN2836714.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            37804..37998
                     /locus_tag="ACG9BB_000745"
                     /pseudo
     CDS             37804..37998
                     /locus_tag="ACG9BB_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(38186..38962)
                     /locus_tag="ACG9BB_000746"
     CDS             complement(38186..38962)
                     /locus_tag="ACG9BB_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN2836715.1"
                     /translation="MNTHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            39085..39492
                     /locus_tag="ACG9BB_000747"
     CDS             39085..39492
                     /locus_tag="ACG9BB_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFN2836716.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT        12876 a         7224 c         8635 g        11099 t
ORIGIN      
        1 taagatacag aacttgtata gaaggctgcc aagtcggtag ataaaggagg tttttatcaa
       61 aaaacatact ttcctttttt ctctattctt ttcttggggg ttttggtaca ctgataggga
      121 atttagatag aagggagcca ataggatgaa gctgaccatc agagatatcg cagaaatggc
      181 aggcgtatca gtaacaactg tctcacagat tttgaataac aaaggaagca gatttagtga
      241 taagacaaga aaaaaagtgt tgtcgatcgt gaatgaacat cattataagc ccgattattt
      301 cgcttcaaat gtgatcaatc gacattcaaa gaccatcgga atgatcgtcc ctgatgtcac
      361 tgatttcttc ttctccaaac tgatcgaagg tgtagagagc tatttgaatc ctttgggcta
      421 tgtgatcatg ttatgcaatt ctcgtcatag ccaagaaaat gagatcaagt atttacaaga
      481 gctttcccac cgctcagtgg acggaatctt gctggccaca ccaaatattt tacctgagaa
      541 atatgcatta gacagcggtt tttatcaaaa aatgccaatc attttaatcg atcgcggatt
      601 gaatcgtcgg gataacggac ggcttatcgt aaaggaatat gagggcgcct atcaagctgt
      661 ttcgctgttt attgaaaatg gccatacgaa gatcggcatg ctgaaggaaa ccactggata
      721 ctatcaatta gaagaacgtt tcaacggata tcgtcatgcg ctgaaagatc atggcctgcc
      781 ttttaatggg aaatttgttg aacatggaga gttgactgtc caaggtggat atactgcatc
      841 aaaaaaatta ctcaaacata aagatatcac ggctattttt tgcgggaatg atgccatggc
      901 gatcggatgc tatcaggcga tcgatgaatt gggcaaaaaa atcccagaag atatctcagt
      961 cataggattc gatggattaa agctgtcaga atacatgatt ccgcaattga cgacagtgca
     1021 gcagccaagt tttgatattg gattttatgc tgcacgtttc ctgatcgata cgatcgaatt
     1081 tcctcaaaga aaagttccga acaaagtgtt cgaaacaaaa ttaattattc gtgagagcgt
     1141 aaaagtattg acaaagggct aactcggcgg tatattgctt atgtaagcat ttacaaaggc
     1201 tagttagtgc tttttttatt tttagtaaac cagtttacta aagtggttta cctttcctaa
     1261 aaataatgta taataccgaa tgaagacaag gaggatcagg caatgcgaat ggtggatctg
     1321 attgaaaaaa agagggatgg aaacgagctg tcgaaagagg aaatcgagta catcgtcacg
     1381 aactatacga atggaaagat tccagattat caagtcagtg cattattgat ggcaattttt
     1441 tatcaagaca tgacaaatga agaaatcacg aacttaacat tggcgattgc taattctggt
     1501 gatgtcatcg atttgagttc tcttgaagga ataaaggtag ataagcattc gacaggtggc
     1561 gttggtgaca cgaccacatt gattttagcc cccctagtag ctagtgtcgg agtgacagtg
     1621 gctaaaatgt ctggacgcgg tctaggttac acaggtggta cactagacaa gctagaagca
     1681 attcccggat ttcagatcga gctttcggat gaagaatttg tacgcattgt taatgaaagt
     1741 aaagtagcgg taattggtca atcaggaaac ttggcaccgg cggacaaaaa attgtatgcc
     1801 ttgcgtgacg tcacagcgac cgttgattct cttccgctga ttgccagttc gattatgagt
     1861 aaaaaaatcg cggcaggagc agatgcaatc gttttggatg tgaccactgg agacggtgct
     1921 ttcatgaaga acatcgaaga tgcaagacgt ctggcaaaga ccatgacgag cattgggaaa
     1981 ctggctaacc gagaaacagt ggcggttatc tcagatatgt cagaacctct tggcgaagct
     2041 atcggaaata gtctagaagt tgtcgaggca atcgaaacat tacaaggaaa tggtccagaa
     2101 gatctagtag aaatgtgcta cgccttagga agccaaatgg ttgttcttgc tggcaaagct
     2161 aagacaaccg atgaagcaag aacgttgctt caagaagctc tggaatcagg taaagcgtta
     2221 gcaaaattca aagaaatgat ccaaaatcaa gggggagacc cagcgatcgt ggaacatcca
     2281 gaacgaattt tgactgcacg ctacacaatg gagcttcctg ctaaacaaag cggagttgtg
     2341 tcaaaaatcg tggcaaatga attaggaatt gctgcaatga tgctaggtgc tgggcgtaaa
     2401 accaaagaag aagatattga tcacgctgtt ggattgaaac tgcataaaaa aattggtgac
     2461 actgtgacaa aaggtgagtc actattgacg atctatagta acgacgaaga aatcacttct
     2521 gtcattgaat tattatataa aaatattgaa attggtgaat cggcaatgaa gccaacgttg
     2581 attcatgaca tcatcacaga ataaaggaaa aaagcggact ttaccgtttt aaagaaaagg
     2641 ggaaaagaaa tggaactaaa ccgaatgatc gaccatacta ttttgaaagc agacgcatct
     2701 aaagaagctg tgatgcaaat tatcgaagaa gcaaaaaaat accattttta ttctgtctgc
     2761 atcaatccag catgggttgc gctagcgaaa gaacaattac aaggtgagcc agtagccgta
     2821 tgtacagtaa tcgggtttcc tctaggagca aacacacccg agacaaaagc atttgaaaca
     2881 acagatgcca tcaataacgg tgcagatgaa gtcgatatgg tcatcaatat cggtgctttg
     2941 aaatcaaagg atgatcaaca agtccaaaaa gatatcgaag cagttgttga agcagcaaaa
     3001 gatcgtgcgc ttgtcaaagt tattattgaa acatcgttat tagatagaga agaaatcatc
     3061 cgagcttgcg aaatagcaaa agctgcaggc gctgattttg tcaaaacatc aactggtttc
     3121 tcaacaggcg gagcaaaggt agaggatgtc cgtttgatgc gtgaaactgt aggaccagaa
     3181 atgggtgtca aagcttccgg cggtatccat aacgaagaag aagcaatggc aatgatcgaa
     3241 gctggtgcta caagaatcgg aacaagcgca ggagtagcaa tcgtatctgg ttcaactggt
     3301 gaaggctatt aattttttca aaggagaagc tcatggcaga actcaaacaa gaatggatca
     3361 acacagctat tgaagcgtta gataaagcct atgttcctta ttcccatttt ccggtaggcg
     3421 cttgtctcgt aacggaatca ggcaagattt atcaaggcat caacattgag aatgcatcat
     3481 ttggattaac gaattgtgcc gagcgcacag cttttttcaa agcagtatct gaaggagaaa
     3541 aaacatttac gcatctagtc gtagcggggc acacccccga tcctatctct ccttgtggtg
     3601 catgtcgaca agtaatggca gaattctgtt cccctgatat gccggttacg ctagtaggag
     3661 acaatggtgt gacgaaggca acgaccgttc gtgcactgct gccttatgca tttactgaaa
     3721 aagatctata actcatgttc attcagtcag ctgactgttg aaaaataaaa caattatttt
     3781 aggaggcttt acagaaatga aaaaagcaaa attatttggt ttaggtgctg tcgcactagc
     3841 agcagggtta tttttaggtg catgcggcaa caacggctct actgattcat ccggtggcaa
     3901 aagctcatca gacacaacaa ctgcagcatt gatcacagac acaggtggtg tagacgaccg
     3961 ttcattcaac cagtcagctt gggaaggact agaaaaatgg gggaaagatc aaggactttc
     4021 acgaggaaat gatggcttcc aatatttcca atcttcaaat gaatcagatt acatcccaaa
     4081 tattgaccaa gcattgaatg ctggattcaa aacgattttt ggtattggtt ataaactaaa
     4141 accagcgatc caagaacaag caacaaacaa tacaggtacg aactttgtta ttatcgatga
     4201 tgtcatcgat ggattagata acgttgtatc tgcaactttc aaagacaacg aagcttctta
     4261 tcttgcaggt gttgcagcag cttatacgac tgaaacaaac gtagtcggct ttatcggcgg
     4321 tgtaaaaggt gaagtaatcg atcgttttga tgctggattc aaagccggtg ttgatgcagg
     4381 tgcgaaagaa ttaggaaaag aaatcaaagt attgaatcaa tatgctggtg acttctctgc
     4441 tccagataaa ggacgttcaa ttgctcaagg tatgtatgca caaaacgcag atatcatctt
     4501 ccatgcttca ggcggtacag gtaacggtgt attccaagaa gcaaaatcat tgaacgaatc
     4561 aggcgacaaa aaagtttggg ttatcggtgt tgaccgcgac caatcagacg aaggcgaata
     4621 cacattaaac ggcgaaaaga aaaacttcac attgacatct acactgaaag cagttggaac
     4681 agtagtagaa gatttggcac aaaaatcagc ggacggtaaa ttccctggcg gcgaacacac
     4741 tgtttatgga ttgaaagaag acggtgttgg tctaacagaa ggacaacttt ctgacgaagc
     4801 gaaaaaagca gttgatgaag caaaagaaaa aatcatttca ggtgacgtaa aagttccaga
     4861 aactcctgaa gaaaactaaa accatttcta tagccttgtt agacacatgt ttgacaaggc
     4921 tatggtttga ttagaacaag aattgttcat ttatccaagc tgccttgtga cagtttggat
     4981 aagggaacaa ctccctgaag taatgtttgt ttcaaaagaa gaacgctcac taagcgtgaa
     5041 tcttagtcaa tcgttgccct agatagaaat cgttaggatg tgaaaaaaag gtggttcaag
     5101 aaaatattgt catagaaatg cagaatatta ccaaagaatt tggaacattc aaggcaaatg
     5161 acaacatcaa tttacaagta aaggcaggag aaattcatgc gttgcttgga gaaaacggtg
     5221 ctggcaaatc tacattgatg aacgtgcttt ccggattatt agagccgaca tcagggaaaa
     5281 ttttgatgcg tgggaaagaa gtaaagatca caagtccgac caaagccaac caattaggga
     5341 ttgggatggt ccatcagcac tttatgcttg ttgatgcctt tactgtaaca gaaaacatcg
     5401 tgttgggaag cgagcctagt cgcgcaggga tgcttgacca taaaaaagca cgaaaagaga
     5461 tccaaaaagt ttctgaacag tatggattgt cagtcaaccc agatgcttac gttcgtgata
     5521 tttcagttgg aatggaacaa cgggtagaaa ttttaaaaac actttaccga ggagcagatg
     5581 tactgatttt tgatgagccg acagctgtat tgacccctca agaaattgat gaattaatcg
     5641 tgatcatgaa ggaattagtc aaagaaggca agtcgatcat tttgattacg cataagttag
     5701 atgaaatcaa agcagtagct gaccgctgta cagttatccg ccgtggaaaa ggaatcggta
     5761 cagtcaacgt taaagacgta acctcacaac aattagccga tatgatggtt ggaagaacgg
     5821 tttcattcaa aacgatgaaa aaagaagcga agcctcaaga agtcgttttg tctattgaaa
     5881 atctagtggt gaaagaaaat cgtggattag aagccgtgaa aaacttgaac ttagaggttc
     5941 gtgctggcga agtacttggt atcgctggaa tcgatggaaa cgggcagtcg gagttgatcc
     6001 aagctttgac tggtttgcga aaggcagaaa gcggacatat caagctaaaa ggggaagaca
     6061 tcactaataa aaaaccccga aaaatcactg aacatggtgt aggacacgtg ccagaagacc
     6121 gtcataaata cgggttagtc ctagatatga cattatctga aaatatcgca ctacaaacct
     6181 atcatcaaaa accttacagt aaaaacggca tgctgaatta ttcagtaatg aatgaacatg
     6241 ccagagaatt gatcgaagaa tatgatgttc gaacaacgaa tgaacttgtt cccgcaaaag
     6301 ctttatcagg cggaaatcag caaaaagcaa ttatcgctcg tgaagtcgac cgagatcctg
     6361 atctgttgat tgttgcaaat ccaactcgtg gactggatgt aggtgcgatc gaatttattc
     6421 ataaacgtct gatcgaacaa agggacaaat acaaagcagt gttactgatc agtttcgaat
     6481 tagaagaaat tttaaatgtt tcggatcgta ttgctgttat ccatgaagga gaaattgtcg
     6541 gtatcgttga tccgaaagaa acatctgaaa atgaattagg tctattgatg gcgggctata
     6601 ccttagaaga agcacgaaaa gagttagaaa agggggctgg cgcacatgaa tgatcgcagc
     6661 gaaaaattcc gtaatttatt agtgcctgta ctttcagttg ttttaggatt gatcttaggc
     6721 gcgatattga tggctgcttt tggttttcag cccatccaag gttattcttc tatgctagat
     6781 gcagcccttg gttcgcaaag agggataggt gaaacgttaa ggcaagcaac gccgttgatt
     6841 tttacagcat taggtttttc tgtagctaac tcagctgggt ttttcaatat tgggctttct
     6901 ggacaagcac tttgcgggtg gatcgtcagt gtctgggtgg cacttgcgtt tccagatctg
     6961 ccaaaagttg ttttgcttcc gttatgcgtg atcttaggtg ccttagctgg tgcgatggca
     7021 gcggctgttc caggtttact tcgagcattt tttggaacaa gtgaagtgat cgtaacaatc
     7081 atgatgaact atatcttatt atacgtaagc acatttgctt tacatgaagt gatgcctgaa
     7141 agctatcgct caagtttgga ctcttccaat atgatcagtc aaaatgcctc actgcggtta
     7201 ccatggttaa cacaagcttt tggcggctct cgcgtaaact caggattatt tttagcaatc
     7261 atcgcgctta ttttcgtttg gctgctgatg aagaaaacga ctttaggatt tgaaatccgt
     7321 tctgttggat tgaatccttt tgcttctgag tatgcaggaa tgagtagtaa acgtacgatc
     7381 gttttgtcga tggtcatttc tggcgctttg gcagggcttg gcggtgtggt tgaaggatta
     7441 ggaacgtatc aaaacttctt tgttcaaacg acttcgctat ctattggatt tgacggaatg
     7501 gctgtttcct tgcttggttc tggatcagca atcggtattc ttttatcagc gatcttgttc
     7561 agtattttga agatcggtgg acttggcatg cagacaggag caggtgttcc gtttgagatc
     7621 gtcaatgtat cgattgcttt gattatcttc tttgtcggga tcaacttcct gattcgtttc
     7681 gtcatggcta aagtcttcca agggaaaaaa caagaagaga tcgttgcgac aatagaatcg
     7741 aaaccaacga atcaaaacca tgaagggggc gaactataat gtcgactatc gaattaattg
     7801 ccttgatcct tacttctaca ctggtttatt caacaccgct aattctaacg tcactaggcg
     7861 gaactttttc tgaacgaagc gggatcgtaa acgtagggct agagggaatc atggtcatgg
     7921 gtgcttttag ttccatcgtt ttcaacttga cgatgagtcc atcacttggt tctatgacac
     7981 catggatcgc tattttagtg ggtggcgtcg taggtatcct attttctctg attcacgcag
     8041 tagcaacagt cagtttgcgt gctgaccata ttatcagcgg gacagtaatt aatctgatgg
     8101 cacctgcttt aggtgttttc ttgatcaaag catggtatgg taaaggtcaa actgataaca
     8161 tcacgcagaa tctggggtat ttttctttcc caggattagc agatattccg gtcattggac
     8221 caattttctt taaaaataca ttcttacctg cttattgtgc tattttgatt gctgtcctag
     8281 cttgggtgat catcttcaag acgaaattcg gcttgcgcct ccgttcagtt ggtgaaaatc
     8341 ctcaagctgc cgacacatta ggtatcaatg tatatgctat gcgttattca ggcgtactga
     8401 tttctggctt gttaggcggt atgggcggtg ctgtttttgc tcagtcgatt tccggtaact
     8461 tttcagctgg tacgatcgtt ggacaagggt tcatctccat ggctgctatg atctttggta
     8521 agtggaatcc tttaggggca atgggtgcct cattgttctt tggttttgct caaagcttga
     8581 gtatcattgg cgcacaatta ccagttattt cttcgattcc gccagtattg ctgcaaattg
     8641 ctccatattt gctgacaatc attgtgctgg ttgttttctt agggaaagct tctggtccaa
     8701 aagcaaacgg gaagaactat atcaaatcaa aataatcttt tagcgaataa aaagggtggg
     8761 ttacgggaag tgtttttcgt ctcgcccttt ttgttttaaa ggcaataaaa ataaaaagaa
     8821 agaaggaata atcatgttta aacgcgtaca tctgatcgtc atggattcag taggaattgg
     8881 agaagcacca gatgccgata agttcggtga tgttggttct gatacattag gacacattgc
     8941 gaaagaagca ggattgaacg ttccacattt agagcagctt ggtttaggaa cgatccgtcc
     9001 tcttgaaggt gtggaatcag tgaaagatca tcaaggctat gcgacgaaat tagaagaagt
     9061 ttctgtaggg aaagatacga tgacaggcca ctgggaaatc atgggattga atatcaaaac
     9121 tccatttcgt gtgtttccta atggattccc tgatgatctg atcaaacaaa tcgaagaatt
     9181 ttctggccgt aaagtcgtat gtaacaaacc ttatagcgga acagctgtta ttgacgatta
     9241 cggtgaacac caaatgaaaa caggcgattt aattgtatat acttctgccg atcctgtcct
     9301 tcagatcgcg gcacatgaag aaatcattcc tttagaagaa ttatatcgga tttgccaatt
     9361 cgttcgtgat ataacaaaag atgatccgta tatgattggc cgaatcattg cacgtccgta
     9421 cgtgggtgaa ccaggagact tcacacgaac aagcaatcgc catgattatg cgttgaatcc
     9481 attcggacac acagtccttg attctttgaa agaagcagga aaggacgtga ttgctgttgg
     9541 aaagatcaac gatattttca acggacaagg aatcactgaa tctgtccgca cgaaaagcaa
     9601 tatggatggc gttgaccagc tgctgaaagt tatgaaaaac gatttttcag gaatcagctt
     9661 taccaattta gtagattttg atgcattata cggtcatcgt cgggatgtag tcggctatgc
     9721 gcatgcgatt gaagaatttg acttacgaat tccagaattg cttgaagcga tggaagaaga
     9781 cgacttgcta atgattacag ccgatcacgg aaacgatccg acgtttactg gtaccgatca
     9841 tacgcgagaa tatgtaccgt tattggttta cagtaaaaaa atgaaagaac aaggggcatt
     9901 gcctcaagga tattattcag atatttcagc aacgattgct gagaacttta gtgtttcttc
     9961 gactgaaaat ggcgaaagct tcttgaaatt attaaaataa tctttttaaa taggagaaag
    10021 caaatgacca agttaagcga gatgttacaa gaaacgaccc agtttatcct tgataaagga
    10081 gtaaaagaaa ttgattttgg tttgattcta ggttcaggat taggtgaatt agcagatgaa
    10141 atcgaagatg ctgtaatgat ttcttatgat gagatcccgt tctttcctgt ttcgacagta
    10201 gttgggcatg caggaaagct agtttatggg tctctggcag gcaagaaagt gttagccatg
    10261 caagggagat tccactacta tgaaggtcat tccatgcaaa cagtgaccta tccagtacgt
    10321 gtgatggccg gactaggggc ccattcagtc attgtgacta atgcatgtgg tggtgtcaac
    10381 gaatcatttg taccaggaga tttgatgctg attaccgatc atatcaattt tactggtgaa
    10441 aatcctttga tcggtccgaa tgaagatgca atgggcccta gatttccaga tatgagtcaa
    10501 gcttatacgc ctgactatca agcaacagca aaagcagcag cagaaaaact aggtttggat
    10561 cttaaagaag gcgtatatat gggctattct ggaccaactt atgaaacgcc tgcggagatc
    10621 cgaatgtctc ggacaatagg agccgatgca gtagggatgt cgactgttcc agaagtcatc
    10681 gtagcagctc acagcggatt aaaagtgtta ggaatctctt gtattaccaa cttagcagca
    10741 ggtatgcaag cgaatctgag ccatgaagaa gttgtagaaa caactcagag agtcaaacaa
    10801 tcttttaaag cattggttaa agaaacattg acattattgt aataggagga acaataaaat
    10861 gagtgtgcat attgaagcga aagaaggcga gatcgccgat aagattttac ttccgggaga
    10921 tcctttgcga gctaaatata ttgcggagac ttttttggaa gatccagttt gttacaatca
    10981 ggtacgcggt atgttaggct ttaccggcaa atataaagga gaacgtattt ctgttcaagg
    11041 aacaggaatg ggaatgccat cagccaccat ctatgcccat gaattgatcc agtcgtatgg
    11101 tgtaaagaaa ttgattcgag taggtacatg cggtgcattg tcaaaagacg tccatgttcg
    11161 tgatttagta ctagcacaag gtgcagcgac cagttcttcg atgatcgaaa aaaatttcca
    11221 agcctttcat tttcctccaa tcagtgattt caatctatta ctgaaagcat atgagatagc
    11281 taaagaaaag ggctatacgg ttcatgtagg aaatgttctt tcggaagatt ctttctacaa
    11341 agatgatttg acagagacgt tccaattagc tgaacttggc gttttaggag tggaaatgga
    11401 agcagcggct ctttattact taggcgcgaa ataccatgtg cagacattaa gtttgatgac
    11461 cgttagcgac catttgatca ccggtgaaga aacaacagct gcagaacgtc aatctacgtt
    11521 caatgaaatg atcgaagtag gattggaaac agcgatagca gaataagaag gaaatgaaaa
    11581 agctgccctg cattaagtaa taagaacatc caagcaaaaa tagctcctca tgtttttgac
    11641 ttggctggac ttattattgc aaatgtaagc ttttgaacac cgtttaccaa gaggttgtga
    11701 atctgccgtt taactctgag tcacaaggaa caatttcaaa aaacagcttt tcatgtttga
    11761 tgaatattgt gacttgtgac cgagaagttg gcaggagaaa accgtttata cgaaaaaagg
    11821 gactgtgaca aatcgtgtca cagtcccttt tttatagtgt tttttatagg tagtattttt
    11881 tagcaactgt taagtcatca tttaattcat aaacaagtgg ttgacctgtt ggaatttcaa
    11941 gatccatgat gtcttcgtca gagatacctt cgatatgttt agctagggcg cgtaatgagt
    12001 taccatgtgc agctactaaa acagttttgt tgtctagtaa tgctggagca atttcatctt
    12061 gccagaatgg caaagcgcgt tctaatgtca ctttcaagtt ttctccacca ggaatatcgc
    12121 gtttgtctaa cattgcgtaa cgacggtcat ttgctgcaga accttcgtca gtttcttcca
    12181 ttaatggagg aagagtatcg taagaacgac gccagatgtg cacttgttcg tcgccgtatt
    12241 tttcagctgt ttcttttttg ttcaaacctt gtaatttacc atagtgacgt tcgtttaagc
    12301 gccatgattt gatttgtggt acccaaagtt gatctgaata ttctaagatc aagttacatg
    12361 ttttgattgc acgagttaaa acagaagtgt aagcaacatc aaattcgatt ccagcttctt
    12421 tgattttgcg tccgccttct tttgcttctt cgattccttc tggtgctaga tttacatcag
    12481 cccaaccagt aaattggttc aatgcattcc attcactaag accgtgacga gaaaaaacta
    12541 attttggcat gtttagctct ccttttaaga taggtttata tttctgaaat tttcagttct
    12601 gttttattgt acaatttttt ggaagagatt tccatcgaaa aattagataa gattctatga
    12661 gaaaacaagt agagaatata aaaaaacgaa cagtgcccac gcaactgttc gtttttaggt
    12721 attagatgca taattgctcg atttcttttt caggtaaatt gatatattta taacatgttc
    12781 caactgaaac accgcatttt gaagaaataa attggatcgt ttcttttttt tcataataga
    12841 gacgtcggat ttttttgatc gtttttgcat cgatttttgg tcttccgccg atttttcctt
    12901 ttttacgagc ttcatctaat ccaactaatg tacgttcttt gatcaatgcg cactccatgt
    12961 ttgctaggtt ttccattaat tggaaataga tttttcccat ttcgcgcgta tcgattcctt
    13021 catcaagact aatcaagtgc agcccgcgat ctttgaataa ttgcgtgagt tctgttaatt
    13081 gccgcgtaga tttgcctaat cgatgaagtt ggcaaatgac tagtgcatcg ccttgttgca
    13141 tgtcagcgat cactgactcc aaatgaacag cagtagaatc attttgttcc acttcatatg
    13201 tctcatggaa aatttcttga caaccaaagt cagataattg atcaacctga gttgttaaat
    13261 caacgtcatt tatggttgtg cgtgcataac caataatttt catggtgatt cctttctagc
    13321 agtgattttc taactggata taacagtaaa aatgtagcat aaagtacgtc aaaaagatag
    13381 ttttttcgca aattttccgc tgtctttttt tattggatga tcaagcagtt tttctttatt
    13441 tacgacggat tgcgtcaaga atctcaacgc ctttttctga gccgacaact actttagaaa
    13501 ccatgtttag gaaaagccca tgttctacta ccccaacaag ttcatctaaa taggtagcta
    13561 agctttctgg ctgttcgatg acttctagat gcagatcaat gatgtaatga ccaccatctg
    13621 tggttaagac ttctccgttt tcgtctgtac gcaagacggg ttggaatctt ttttcttcaa
    13681 aaatatgaag tagctgttgg gagccataag ggatcacttc aactgggagt gggaacttcc
    13741 caagtttatc gactaattta gaagaatcaa cgatccaaat cgtttctttt gagtacgtag
    13801 cgactatttt ctcgaaaagc aaagcagcgc cgccgccttt gatcccttga aaatcagcac
    13861 tgatttcatc agcaccgtct attgtcaaat cgactacggg gacttcatca atactcttta
    13921 acggtattcc taatttttcc gcttgttctt ttgtcgcatt tgaagtagta actcctgtga
    13981 tacgtaaacc ttcttcacgc atacggcgac caatctcttc taccatataa taggcggttg
    14041 aaccggtccc gagacctacg atcatcccgt cctttacgaa tttcgcggct tcaatgccag
    14101 ctaattgttt taaattcatc tttgcgtcca ccttttttta gtttgagcat cgtgctttcc
    14161 tcactatttt agcgtgattt acggtaaaaa gataccattc ttttttattc caataaagct
    14221 ttttcaaaaa aaattgcatc tcttgttgac atgttttttc attcgtgata ttctaacaaa
    14281 ggtgctttat ctacaggttt ctgtgaagaa acgtgctgac tgtttatcaa agcatatttg
    14341 ataaggatgc agacattgca tttatttttt atcacaaaaa aagaaccacc tggatgtgtg
    14401 gaactagacg caaatcgtgg gaaggaggaa acaaggaatg cctacaatta accaattagt
    14461 acgtaaacct cgtaaatcaa aggtggaaaa atctaattca cctgctttga acaaaggata
    14521 taacagcttt aagaaaactc aaactaacgt aaactcacct caaaaacgtg gggtagcaac
    14581 tcgtgtgggt acaatgacac ctaaaaaacc gaactcagct ttacgtaaat atgcacgtgt
    14641 tcgtttgtca aacttaattg aagttactgc ttatatccca gggattggtc ataacttaca
    14701 agaacacagt gttgtattat tacgcggtgg tcgtgtaaaa gacttaccag gggtacgtta
    14761 ccatatcgtt cgcggtgctt tagatactgc tggtgttaac gaccgtaaac aaagccgttc
    14821 taaatacgga actaaaaaac ctaaagctta attttaatta ttaaacaaat gaaacttgct
    14881 tctactatat agaagtaatt actatgaaca atatttcgga aggaggagtt acggatgcca
    14941 cgtaaaggtc ctgttgcaaa acgtgatgtt ttaccagatc ctatttataa ctcaaaatta
    15001 gtaactcgct tgatcaaccg tgtaatggtt gacggaaaac gcggtattgc agctaacatt
    15061 atctataatg cctttgactt gatcaaagaa tctacaggta acgatccatt agaagtgttt
    15121 gaacaagcaa tgaaaaacgt tcaacctgtt cttgaagtta aagcacgtcg tgttgggggt
    15181 tcaaactacc aagtaccagt tgaagttcgc cctgaacgcc gcgttactct aggtcttcgt
    15241 tgggtagtaa actacgctcg cttgcgcggt gaacacacaa tggaagaacg cttagcaaaa
    15301 gaaatcatgg atgctgcaaa caacacaggt gcttctgtta aaaaacgtga agacactcat
    15361 aaaatggctg atgccaaccg tgcgtttgct cactatcgct ggtaagattc tctctgtttg
    15421 gtcggctttt ataagtcgac ctcagatagt gtccttattt ttagcaacta gatagtaaag
    15481 agaggagaaa caaggagaat gaccagagaa ttttctttac aaaacactcg taatatcggt
    15541 atcatggccc acatcgatgc tggtaaaacg actgctactg aacgtatctt gtactatact
    15601 ggtaaaatcc ataaaattgg tgaaactcat gaaggagctt cacaaatgga ctggatggaa
    15661 caagaacaag aacgtggtat tacgatcact tctgctgcta ctacagcaca atggaagggt
    15721 caccgaatca acatcatcga cacaccagga cacgtagact tcacagttga agttgaacgt
    15781 tcattgcgtg tacttgatgg tgctgtaact gttcttgatg cacaatcagg tgttgaacct
    15841 caaactgaaa cagtttggcg tcaagcaaca acttatggtg tacctcgtat cgtatttgca
    15901 aacaaaatgg acaagatcgg tgctgacttc ttatattcag tatcaacttt acatgaccgt
    15961 ttgcaagcaa acgctcaccc aatccaatta ccaattggtg cggaagacga cttcacagga
    16021 attatcgacc ttgtaacaat gaaggctgaa atgtatacaa acgatcttgg aacagagatc
    16081 gaagaaacag aaattcctga agaataccgc gaattagctg aagaatggcg cgaaaaattg
    16141 gttgaagctg tagctgaaac tgatgaagaa cttactttga aatacttaga aggtgaagaa
    16201 atcactgaag ctgagttaaa agaaggtatc cgccgcgcaa cagttaacgt agaattctac
    16261 ccagtactat gtggttcagc atttaagaac aaaggggttc aattactatt ggacgctgtt
    16321 cttgattact tgccatcacc gttagatatc ccagctatca aagggattga ccctaaaact
    16381 gatgaagaag tagaacgtcc tgcagacgac agcgctcctt tctcagcttt agcatttaaa
    16441 gttatgactg acccattcgt aggtcgttta actttcttcc gtgtgtactc tggtgtttta
    16501 caatcaggtt catacgtaca aaacgcaaca aaaggcaaac gtgaacgtgt tggtcgtatc
    16561 ctacagatgc atgccaactc tcgttcagaa atctctgaag tttacgctgg agatatcgct
    16621 gctgctgttg gtttgaaaga cacaactact ggggacacac tatgtgatga aaagagtcta
    16681 gttattcttg aatccatgga attcccagaa ccagttatcc aagtcgctat tgaacctaaa
    16741 tcaaaagctg accaagataa aatgggtgtg gctttacaaa aactatctga agaagatcct
    16801 actttccgtg ctgaaacaaa cgtggaaaca ggcgaaacaa tcatcgctgg tatgggtgaa
    16861 cttcacttag atatcatcgt tgaccgtatg cgtcgtgaat tcaaagttga agctaacgta
    16921 ggtgcgcctc aagtatctta ccgtgaaact ttccgtgctg gcactcaagc cgaaggtaag
    16981 tttgtacgtc agtctggtgg taaaggtcaa tacggtcacg tatggattga atttacacca
    17041 aatgaagaag gtgctggttt cgaatttgaa aatgcgatcg tcggtggggt tgttcctcgt
    17101 gaatacattc cagctgttga aactggtttg aaagatgcta tggaaaatgg tgtcttagct
    17161 ggctatccat tagtagatat caaagctaaa ctttacgatg gttcatacca tgatgtcgat
    17221 tcaaatgaaa ctgccttccg tgttgctgct tctatggcat tacgtgcagc agctaagaaa
    17281 gctaatccag taatccttga accaatcatg gctgtagaag ttgttatccc tgaagattat
    17341 ttaggtgatg ttatgggtca cgtaactgct cgtcgtggac gtgttgaagg tatggaagct
    17401 cgcgctggcg gccaacaagt tgttcgtgcg atggttccac ttgctgaaat gtttggttat
    17461 gctacaactc ttcgttctgc tactcaaggt cgtggtacat ttacaatgac atttgaccac
    17521 tacgaagatg taccaaaatc agtacaagaa gaaatcatta agaaaaacgg cggaaaagct
    17581 gaataatttc ttgaaaccgg atagtcctgc aaagagatta tccaaagaga ctatattttt
    17641 aatatagttt cgtgtaaaat aattatcgat gatatgggca tgtggtgtac cttgtgtaca
    17701 tcatatacct atcagacaaa taaaaaatat attgtatttt taggaggaca ttttaaaatg
    17761 gcaaaagaaa aatttgaccg ttctaaacca cacgttaaca ttggtactat cggacacgtt
    17821 gaccatggta aaactacatt aacagctgca atcacaactg tactatctaa gaaaaacggc
    17881 ggccaagcta tggcttacga tcaaatcgat ggtgctccag aagaacgcga acgcggaatc
    17941 acaatctcaa cagctcacgt tgagtatgaa acagatacac gccactacgc tcacgttgac
    18001 tgcccaggac acgcggatta cgttaaaaac atgatcactg gtgctgctca aatggacgga
    18061 gctatcttgg tagtttctgc tgctgacggc ccaatgcctc aaactcgtga acacatccta
    18121 ttgtctcgtc aagttggtgt tccttacatc gttgtattct tgaacaaagt agacatggtt
    18181 gatgacgaag aattactaga attagttgaa atggaagttc gtgacctatt aacagaatac
    18241 gaattccctg gtgacgatgt tcctgtagtt gctggatcag ctttgaaagc tctagaaggc
    18301 gacgcttcat acgaagaaaa aattcttgaa ttaatggctg cagttgacga atacatccca
    18361 actccagaac gtgacaacga caaaccattc atgatgccag ttgaagacgt gttctcaatt
    18421 actggacgtg gtactgttgc tacaggtcgt gttgaacgtg gacaagttcg cgttggtgac
    18481 gaagttgaag ttgttggtat tgctgaagaa acttcaaaaa caacagttac tggtgttgaa
    18541 atgttccgta aattgttaga ctacgctgaa gctggagaca acattggtgc tttactacgt
    18601 ggtgttgcac gtgaagacat ccaacgtgga caagttttag ctaaaccagg tacaatcaca
    18661 cctcatacaa aattctctgc agaagtatac gtgttgacaa aagaagaagg tggacgtcat
    18721 actccattct tcactaacta ccgtccacaa ttctacttcc gtacaactga cgtaacaggt
    18781 gttgttgaat taccagaagg aactgaaatg gttatgcctg gcgacaacgt aacaatggaa
    18841 gttgaattaa tccacccaat cgctatcgaa aacggtacta aattctcaat ccgtgaaggc
    18901 ggacgtacag ttggtgccgg tgtcgttaca gaaatcaaag cttaattcac ttaagtttta
    18961 aagtaaagca acatttattc ggacgtaatc gctcggaacg aaggtcttag gatctttgtt
    19021 ccgagctttt ttatttattg caggatcagg aaaaaagaca ggagtattat aaatagaaga
    19081 aacacgaaat aaaagagtca gtattttcta aatgaaactt tatgaaaaca aaatgttttt
    19141 tacaagaaaa agccttgctt tgtgagttta tatatattat aatgacaaag gtgcgtttac
    19201 tttagtgaat tgcacagacg taatcagaat gatttcgtcg gctaagaaac gagaggttgc
    19261 gacacgcccg gacgctttgc cacgaacgag cgtgacggaa attttcgtgg agctatgtct
    19321 acttttcaaa ataggcgaag gagggaacaa gatggcaaaa caaaaaattc gtatccgttt
    19381 aaaagcgtat gaacaccgta ttttagatca atcagcggat aaaatcgtgg aaactgcaaa
    19441 aagaactgga gctagcgtgt ctggtccaat tccattacca actgaacgca gtctttacac
    19501 aatcatccgt gcgactcata aatacaaaga ttcacgcgaa caattcgaaa tgcgtacaca
    19561 caaacgtctt atcgacattg tgaacccaac accaaaaact gttgatgctt tgatgaagct
    19621 agacttacca tctggtgtaa acatcgaaat caaattataa aactaaaaca aaaataatgg
    19681 aggtgtactc atgaccaaag gaatcttagg gaaaaaagtg ggaatgacac aaatcttcac
    19741 tgaatctggt gaattaattc cagtaactgt tgtagaagct actccaaacg tagtactaca
    19801 agtgaaaaca atggaaacgg atggctacga agctattcaa gttggttacc aagatatgcg
    19861 tgaagtttta tcaaacaaac ctgcgaaagg tcatgttgca aaagcaaaca cggctcctaa
    19921 gcgcttcatt cgtgaattca aaaatgttga gctaggagaa tatgaagtag gaaaagaaat
    19981 tacagtagat gttttccaag caggagacat tattgatgta acaggtacta cgaaaggtaa
    20041 aggattccaa ggggttatca aacgccacgg acaaagccgc ggaccaatgg ctcacggttc
    20101 tcgttaccat cgtcgtcctg ggtcaatggg tccagttgca cctaaccgtg tatttaaaaa
    20161 caaacgtctt gctggccgta tgggtggcaa ccgcgtaact atccaaaact tggaagttgt
    20221 acgtgtcgat gcagaaagaa atgttatctt gatcaaaggt aatgttcctg gagctaaaaa
    20281 atctttaatt actatcaaat cagctgtgaa agctaaataa ttgtaggaag aaaggaggaa
    20341 ctaaggaatg ccgaatgtag cattattcaa acaagatgga agccaaaacg gtgaaatcac
    20401 acttaacgaa gaaatcttcg gaattgaacc taatgaaagt gttgtttacg atgcaatcgt
    20461 aatgcaacgt gcgtcattaa gacaaggaac acacgcggtt aaaaaccgta gcgctgttcg
    20521 cggcggcgga cgtaaaccat ggcgtcaaaa aggaacaggt cgtgcacgtc aaggatctat
    20581 ccgctcacca caatggcgtg gaggtggcgt agtctttgga ccaacaccac gttcttacag
    20641 ctacaaactt cctaaaaaag ttcgtcgttt agcaatgaaa tctgtattgt cagaaaaagt
    20701 tgctgaaaac aacttggtag caatcgaagg cttgaacttc gacgcaccaa aaacaaaaga
    20761 attcaaacaa gttcttgcta acttatctat cgactctaaa gtattggttg ttctagaaaa
    20821 aggtaacgac tttgctgctt tatcagcacg taacttacca aacgtttctg tagtaacttc
    20881 tgataatgtg agtgtgctag acgttgtatc aaatacaaaa gtgttagcaa cacaaactgc
    20941 tcttactcaa attgaggagg tgcttgcata atgaacttac tagacgtaat caaacgccca
    21001 gtgatcactg aaaaatcaat gcttgccatg gatgacaaga aatacacttt tgaagtggac
    21061 actcgcgcaa acaaaacttt agtaaaacaa gcggtagaag ctgcttttga cgttaaagtc
    21121 aaaaacgtga acatcgtgaa cgtacgtgcg aaattcaaac gtatgggcaa atatgcagga
    21181 tatacaaaaa aacgtcgcaa agcgatcgtt acattgactg aagattcaaa agaaatccaa
    21241 ttattcgaag ctgctgaata agcactaata gataactcat taaattagga gggaaaaacg
    21301 tggcgattaa aaagtacaaa cctacctcaa atggtcgtcg taacatgaca ggttctgatt
    21361 ttgctgaaat cacaacatca acacctgaaa aatcattgtt acaaccattg aaaaaccatg
    21421 ctggacgtaa caacaacggc cgcatcactg tacgtcatca aggtggcgga cacaaacgcc
    21481 aatatcgcgt gatcgacttc aaacgtaaca aagataacgt tgttgcaact gttcaaacga
    21541 tcgaatatga tccaaaccgt tcagctaaca tcgcgttagt acactatgaa gatggagtga
    21601 aagcttacat cttagcacct aaaggactag aagtgggtat gaaacttgtt tcaggtccag
    21661 aagcagatat caaagtcgga aatgctcttc cattagaaaa cattccagtt ggtactgtta
    21721 tccacaacat tgaaatgaaa cctggtaaag gtggacaatt gatccgttct gctggtacaa
    21781 gtgctcaagt acttggtaaa gaaggcaaat acgtattgat ccgcttgaac tctggtgaag
    21841 ttcgtatgat cttagctgca tgccgtgcta caatcggttc tgtaggtaac gaacaacacg
    21901 aattaatcaa catcggtaaa gctggacgct ctcgttggat gcgtaaacgc ccaactgtac
    21961 gtggtagcgt aatgaaccct aatgatcacc cacacggtgg tggtgaaggt aaagctccta
    22021 tcggacgtaa agcaccagtt tctccttggg gtcaaccagc tcttggttac aaaactcgta
    22081 acaaaaaagc taaatcagac aaacttattg ttcgtcgtcg caaaaataaa taattacttt
    22141 gaccatgtgt cgcacatttt gagaggaggt tcaccatggg tcgtagttta aagaaaggac
    22201 cttttgtcga tgagcattta atgaaaaaag ttgaagctca agcaggggtt gaaaagaaaa
    22261 aagttatcaa aacttggtca cgccgttcaa cagttttccc aagttttgtt ggatatacca
    22321 tcgcagttta tgatggacgt aaacatgtac cagtttacat ccaagaagac atggtaggac
    22381 acaaattagg tgaatttgca cctacaagaa cttatcgtgg acatgctgct gacgataaga
    22441 aaacgaaacg ctaatttgag gggaggataa atccaaatgg cagaacaaat cacatcagct
    22501 aaagcaactg caaaaacagt tcgcacttca cctcgaaaag cccgtttagt aattgacctt
    22561 atcagaggta aaagtgtcgc agatgcaatt tcaatcttga aattcacacc aaacaaatct
    22621 gctggaatca ttgaaaaagt attgatgtca gcagttgcga atgcagaaaa taactttgac
    22681 ttagatgttg aaaacttggt agtaagcgaa gcttttgtta acgaaggacc tacaatgaaa
    22741 cgtttccgtc ctcgtgcaaa aggatcagct tctccaatca acaaacgtac aagtcatatc
    22801 acggtagtcg tatcagaaaa ataaggaggg ataatcagtg ggtcaaaaag tacatccaat
    22861 tggaatgcgt gtaggcatca tccgcgactg ggatgctaaa tggtatgctg aaaaagagta
    22921 tgctgagttc ttacacgaag atttaagaat ccgtaaattt atttcaacta aacttgctga
    22981 tgctgctgta tcaacagttg aaatcgaacg cgcaacaggt cgcgtaaaca tttcaattca
    23041 cacagctaaa ccaggtatgg ttattggtaa aggcggttct gaagtcgaaa gcctaagaaa
    23101 agaattaaac aaattaactg gtaaaagaat ccatatcaat atcgtggaaa tcaaaaaacc
    23161 agatctagat gcaaaattag taggtgaagg aatcgcacgt caattagaaa accgtgttgc
    23221 tttccgtcgc gctcaaaaac aagccatcca acgctcaatg cgtgctggcg ctcaagggat
    23281 caaaactcaa gtatctggtc gtctaaacgg agcagatatc gctcgttcag aaggatactc
    23341 agaaggaact gttcctcttc atactttacg tgcggacatt gattacgcat gggaagaagc
    23401 agatacaaca tacggaaaac taggagttaa agtgtggatc tatcgtggag aaattcttcc
    23461 aactaaaaaa aacactgaga aaggagggaa ataatcatgt tagtacctaa acgtgtaaaa
    23521 caccgtcgtg aattccgcgg aaaaatgcgc ggggaagcta aaggcggaaa agaagtagca
    23581 ttcggtgaat acggtttgca agctgttgat tcacattgga tcacaaaccg ccaaatcgaa
    23641 gctgctcgta tcgcaatgac tcgttacatg aaacgtggtg ggaaagtatg gattaaaatt
    23701 ttccctcaca aatcttatac tgccaaagca attggggtac gtatgggttc tggtaaaggg
    23761 gcacctgaag gatgggttgc accagtaaaa cgtggtaaaa tcatgtttga aatcgcaggc
    23821 gttcctgaag aagtagctcg tgaagcgtta cgtctagctt ctcacaaatt accaatgaaa
    23881 actaagatcg taaaacgtga ggaaatgggt ggtgaatcga atgaaggtta aagaaatcag
    23941 agaattaacc actgccgaaa tgcttgatca agaaaaacaa ttaaaagaag aattgtttaa
    24001 tcttagattc caattagcaa caggtcaatt agaaaacact gcacgtatta aagaagtacg
    24061 taaatcgatt gcacgcatca aaactgtgtt gcgtgaacaa gctaagtaat tatggaagga
    24121 ggccattaag cgtatgactg aaggaagaaa tcaacgcaaa gtttatcaag gtcgcgtggt
    24181 ttcagacaaa atggataaaa caattaccgt tgctgtcgaa acaaagaaaa accaccctat
    24241 ctatggcaaa cgcatgaact attctaagaa atacaaagtg catgatgaaa acaacacagc
    24301 aaaagttggc gacatcgtga gaattatgga aactcgtcca ttatcagcta caaaacgttt
    24361 ccgtttgttg gaagtagtcg aagaagcagt tattatctaa taacacgaga ttttcctata
    24421 tagattgaat gcaaaatctg aaaggaggat acacaacgtg atccaagcag aaagtcgttt
    24481 aaaagtcgct gataactcag gcgcacgtga aattttgacg atcaaagtcc ttggtggatc
    24541 tggtcgtaaa actgctaaca tcggtgatgt gatcgttgct acggttaaac aagcaacgcc
    24601 aggtggggtt gtcaaaaaag gtgacgtcgt taaagccgtt atcgttcgaa caaaatcagg
    24661 agctcgtcgt acagacggtt catatattaa atttgatgaa aatgctgcag tgattattcg
    24721 tgatgataaa agccctcgtg gaacacgtat ctttgggcca gttgcacgtg aactacgtga
    24781 aaacaacttc atgaagatcg tttcactagc accagaagtt ttataatctt gagacactta
    24841 tcaaaggagg tgcgaaacag ttatgtttgt taaaaaaggc gataaagtta aagttatcac
    24901 tggaaaagac aaaaacaaag aaggcgttgt actagcagcg ttcccgaaaa aagataaagt
    24961 tattgtcgaa ggtgtaaatg tcgttaaaaa acaccaaaaa ccttctcaag ctgctcctca
    25021 aggtggaatt gttgagatgg aagcgccaat tcatgtttct aacgtgatgg tggttgactc
    25081 tactggtgta gctggccgag ttggctacaa agaagtagac ggcaaaaaag tccgtgtttc
    25141 taaaaaaacc ggtgaagttt tagataaata aaagtttaag gaaggagggt cttactgaat
    25201 gaaccgccta aaagaaaaat atcttaaaga agtaactcca tcattgatgg aaaaatttaa
    25261 ctatagctct gttatgcaaa cacctaaagt tgaaaaaatc gtcatcaaca tgggtgttgg
    25321 tgatgcggta tcaaacgcta aaaacttaga taaagcagtt gaagaattaa ctttgatcac
    25381 tggtcaaaaa ccaatgatta caaaagctaa aaaatcaatc gctggattcc gtttacgtga
    25441 aggaatgcca attggtgcaa aagttacatt gcgcggagaa agaatgtacg aattcttaga
    25501 caaattggtt tctgtttcac taccacgtgt acgtgacttc catggtgtaa gtaaaaaagc
    25561 ctttgacggt cgtggaaact atactttagg tattaaagaa caattgatct tcccagaagt
    25621 agattatgat ttagtagata aagtacgtgg tatggacatc gttattgtaa caacagcaaa
    25681 ctctgatgaa gaatctcgtg agttgttgac acaattaggc atgccattcc aaaaataatt
    25741 aaaggaggcg aactacgtgg ctaaaaaatc aatgattgct aaaaacaaac gtcctgcaaa
    25801 attttcaaca caagcttata ctcgttgcga acgttgcgga cgtccacatt cagtttatcg
    25861 taagttccat ctttgccgta tttgcttccg cgaacttgcc tataaaggtc aaattcccgg
    25921 cgtgaagaaa gctagctggt aaaaagtaaa ttcgcataag aaggaggtaa aagatcaatg
    25981 gtcatgacag atccaattgc agattttcta actcgtatcc gtaacgcaaa catggttaaa
    26041 catgagtctt tagaagtacc tgcatcaaaa attaaacgtg acatcgctga aatcttgaaa
    26101 cgtgaaggtt tcgttcgtga tgttgaatac atcgaagatg acaaacaagg cgtgatccgt
    26161 gttttcctaa aatttggtaa aaacggcgaa cgtgttatca caaacttaaa acgtatctct
    26221 aaaccaggtt tgcgtgctta tgtcaaagct gacgaagtac caaaagtatt gaacggttta
    26281 ggtatcgcaa tcatttcgac ttctgaaggt gttgtcactg ataaagaagc tcgtgaaaaa
    26341 aatatcggcg gcgaagttat cgcttacgta tggtaatcaa gtagaatcta caaggaggtg
    26401 tctttaagtg agccgtatcg gaaataaagt cgttgttatc cctgaaggag taacagttac
    26461 tcaagacgga aacaacgtta cagttaaagg accaaaaggt gaattgactc gtactttttc
    26521 agcagatatc aaaatgaata tcgaaggaaa cgaagtaact ttcactcgtc caaacgacag
    26581 taaagaaatg aaaacaattc atggaacaac tcgtgcgaac ttcaacaaca tggttgtagg
    26641 tgtcagcgaa ggtttccaaa aagcgttaga actaatcggg gttgggtacc gtgcacaatt
    26701 acaaggatca aaacttgtat tgaacgttgg ttactcacat ccagtagaaa tcacaccacc
    26761 agctggagta actgttgaag taccagctaa cacacaagta attgttaaag gtgcgaacaa
    26821 agaagaagtt ggcgaattag ctgcaaacat tcgtggaact cgtcctccag aaccttataa
    26881 aggtaaaggt attcgttatg ttggcgaata tgtacgccgt aaagaaggta aaactggtaa
    26941 ataatcgttt agaaaaattg catagaagcc agcagcagga aaaaggcatt tggctgaagt
    27001 ttaccttcaa aaaaatacat ttttacttgt gccgcagctt ctatttttcg aaacgtattt
    27061 actgcttgaa atagtagtac aataaaaata aagaggtgac aattgtgatt acaaaaccag
    27121 ataagaacaa aacacgtcaa aagagacatc gtcgtgtacg taacaatatc tctggtactg
    27181 ctgagcgccc acgcttgaac gtttttcgtt ctaacaaaaa catctacgct caaattattg
    27241 atgacgtagc gggtgtaacg ctagcaagtg cctctacctt ggataaagaa atttcaggtg
    27301 gaacaaaaac agaagcagca caagctgtcg gtaaactagt tgctgaacgt gcagctgaaa
    27361 aaggcattaa agtagtagtc tttgaccgtg gtggatacct ttaccatggc cgtgtgcaag
    27421 ctttagctga agctgctcgc gaaaatggac tagaatttta gaagaaggag gaataccatt
    27481 catggtttat atcgatccaa aacatttgga attagaagac cgcgttgttg caattaaccg
    27541 tgtaacaaaa gttgttaaag gtggacgtcg tctacgtttt gctgctttag ttgttgtcgg
    27601 tgacaaaaac ggacacgtag ggtttggtac aggtaaagct caagaagttc ctgaagcaat
    27661 ccgtaaagca attgaagatg cgaagaaaaa cttagttgaa gtacctatgg ttggttcaac
    27721 aatcccacac gaagtaatcg gtgcttttag tggtggtaga atcctaatga aacctgctgt
    27781 agaaggttct ggggttgctg ctggtggacc agttcgtgcc gtattagaat tagctggggt
    27841 agcagatatc acttctaaat ctttaggctc aaatacacca atcaacgttg ttcgcgcaac
    27901 agttgaaggt ttgaaacaat tgaaacgtgc agaagaagtt gcagaacttc gcggcaaatc
    27961 agttgaagaa ttaatcggat aaggaggaca acaataatgg ctgaattaaa agttacttta
    28021 aaacgcagta ttatcggacg tcctcaaaac caacgcgata cagttaaagc gttaggtctt
    28081 acaaagatta acagttcagt ggttaagcct tctaatgaag caattaaagg catgatcaat
    28141 actgtttctc acctagtgga cgttgaagaa atttaattta gaaaaatcat aactagaaca
    28201 ttaaggaggt gccaaaccta tgaaacttca tgaattaaaa cctgctgaag gttcacgcca
    28261 agtacgtaac cgtgttggtc gtggtacttc atctggtaac ggaaaaactg ccggacgcgg
    28321 acaaaaagga caaaaagctc gttcaggcgg cggtgtgcgt ttaggatttg aagggggaca
    28381 aacaccattg ttccgtcgtt taccaaaacg aggattcaca aacgtgaacc gtaaagacta
    28441 tgcagtcatt aatcttgatg tattgaaccg ctttgaagac ggagctgaag taactcctgt
    28501 tgctttagtt gaagctggaa tcgtgaaaaa cgaaaaagct ggaatcaaag tattagctaa
    28561 tggggaacta aacaagaaat taactgtgaa agcagcaaaa ttctctaaag cagctcaaga
    28621 agcaattgaa gcggctggtg gatcaatcga ggtgatctaa tgtttagact attaaaagat
    28681 gcttttaaag taaaagacat tagatcgaaa atcctattta ctgtatttgt gttgtttgta
    28741 tttcgcctag gtgcacatat tactgtacca ggcgtaaatg ctgcttcatt gcaaaacctt
    28801 tcttctttgc catttttcaa catgttgaac ttggtaagtg gtagtgcgat gcaaaacttt
    28861 tccattttct caatgggggt ttcaccatac attactgctt ctatcgtcat tcagcttctt
    28921 caaatggaca tcgtgcctcg atttgttgaa tggtcgaaac aaggggaagt tggtcgtaaa
    28981 aaactgaatc aagcgactcg atatctgaca ttagtattgg catttatcca atctgtcggt
    29041 atcactgctg gattcaatca atacgctcaa ttaggtttcg tgaataatcc taacgtagca
    29101 acttacgtaa cgatcggttt gattctaaca gccggtacga tgtttgttac ttggctagga
    29161 gaacagatca cagaaaaagg tatcggaaat ggcgtatcaa tgatcatttt tgcaggtatc
    29221 atttctagat tgcctgaaag tatcagagaa atttatgaag attactttat aaatattgat
    29281 ccttcagaca tatggaaatc tgctattttt gtggccattt tactagtagc ggtcttgttg
    29341 atcgtgacat tcgtaacatt tttccaacaa gctgaacgca agatcccgat ccaatatacg
    29401 aaacgcgtag ctggcgcgcc tacaagcagc tatctgccgt tgaaagtaaa tgcagctgga
    29461 gttattccag ttatctttgc tagttcgttt attgctacgc caaatgctgt tttacaagca
    29521 ttgagcggta gattcggttc tgaaaactgg tacaaagtga tgatggagat ctttaactat
    29581 aatacagcac ctggtgctac gatttatacg attctgatcg ttgccttcac tttcttctac
    29641 gcctttgttc aagtaaaccc tgagaaatta gcggaaaact tgcaaaagca aggaagttat
    29701 ataccaagcg tacgccctgg taaggggact gaagagtatg tctcaagatt actgatgcgt
    29761 ttaagtacag ttggtgcagt cttccttggt ttagtggcat tgcttccaat cattgctcaa
    29821 atggtttggg atcttccaca atcaatcgga ttaggcggta caagcttact gatcgttatc
    29881 ggtgtagctt tagaaactgc gaaacagtta gaaggcttaa tgctgaaacg taaatatgtt
    29941 ggattcattg agtaagaaag cgtgttgagg tttcccctca acacacgttc tcacacattt
    30001 attaggaggg aaaacaatga acctcatttt aatggggctg ccaggcgcag gtaaaggaac
    30061 acaagctgaa aaaatcattg atgcttatgg tattccgcac atctcaacag gagatatgtt
    30121 ccgcgcagcg atgcaaaacg aaacagctct tggcctggaa gcaaagtctt atatggataa
    30181 aggtgcatta gttcctgatg aagtaacaaa tggaatcgtg aaagagcgac tagctgaacc
    30241 agatacagag aaaggcttct tattagatgg ctttccacgt acgctagatc aagcaaaagc
    30301 tctggacgca atgctaaaag atttgaataa aaaaattgat gctgtcatcg atatccatgt
    30361 tggtgaagaa atcttggtcg aacgtctagc cggccgattt atctgcagca attgtggagc
    30421 aacttaccat aaaattttca accctacaaa agttgaagac acatgtgatc gttgcggtgg
    30481 gcatgaattc tatcaaagag aagatgataa acctgagacg gttaaaaatc gtctggctat
    30541 caacatcaaa aatagtgaac caatcttggc ttattataaa gaacaaggtt tgctaaacac
    30601 tattgatggt gctcgtgaaa tcgatgctgt atttgcagat gtacaaaaaa tcattgaaaa
    30661 atagcacgat tgcccgtagc cgacttgcat tatgcagtta ctcttgtcaa gttcaataaa
    30721 ttatgataga atatgtcagt ccgacttcta gagaatcctc tagatgatcc attttgagaa
    30781 ttttgaggtg ggaactgcag tttccgccat tttatcgtgt gaaaatgcga aacaagtaca
    30841 aggaggtact gtgcgtggca aaagaagata tgattgaagt cgaaggtaca gtcgtcgaaa
    30901 ctttgccgaa tgcaatgttt aaagttgaac tagaaaacgg acaccaagtt cttgctactg
    30961 tttcaggtaa aattcgtatg cattacatcc gtatcttacc tggtgacaaa gtaacggttg
    31021 aattgtctcc atacgatctt aaccgtggcc gtattactta tcgctttaaa taattgtact
    31081 ccgtaaaact acaggaggta ttatcatgaa agtaagacca tcagtaaaac caatgtgcga
    31141 acattgtaaa gtaattcgtc gtaaaggacg cgttatggtg atttgcccag caaatccaaa
    31201 acataaacaa cgtcaaggat aattggaggt gctataaata tggctcgtat tgcaggagta
    31261 gatattcctc gtgataaacg tgtagtagtt tcacttactt atatctatgg tattggtaac
    31321 actactgcta aaaagatctt agctgatgct ggtgtatctg aagatgttcg cgttcgtgaa
    31381 ttaacgaatg aacaaacaga tgctatccgt gcagaagtag ataaattaaa agttgaaggg
    31441 gatcttcgtc gtgaagtgaa cttgaacatc aaacgtttga tggaaatcgg ttcttaccgt
    31501 ggaatccgtc atcgccgtgg attgcctgtt cgtggacaaa acacgaaaaa caatgcacgt
    31561 actcgtaaag gcccagctcg cgctatcgct ggcaagaaaa aataattctt aagtgaagga
    31621 ggttaaaact tcatggcagc aaaaaaagtg aatcgtaaac gccgtgtgaa gaagaatata
    31681 gaagcaggta ttgcacatat ccattctaca ttcaataaca ctatcatcat gatcactgat
    31741 actcacggaa atgcattagc gtggtcatca gctggttcat taggtttcaa aggaagcaaa
    31801 aaatcaactc cttttgccgc tcaaatggca gcagaaactg ctacaaaagc agcaatggaa
    31861 cacggattaa agactgttga agtaacagtt aaaggacctg gttctggacg tgaagcagca
    31921 attcgttcat tacaagctac aggtttagaa gtgactgcaa ttcgtgacgt gactccagtt
    31981 cctcataatg gtgcccgccc tccaaaacgc cgtcgtgttt aatgagtgcc gctcattttg
    32041 agttttatca gtaacgtcat tgaacaagac acttttcgtt ttgaaagggg taaagataag
    32101 aatgattgag ttcgaaaaac caagaatcgc aaaaattgat gaagaaaaag attatggcaa
    32161 gttcatcgtt gaacctcttg aaagagggta tgggactact ttagggaatt ccctacgtcg
    32221 tattttatta tcttctttgc caggtgctgc catcactaat attcaaatcg atggtgtact
    32281 gcatgaattc tctaccatca aaggtgttag agaagacgtc actcaaatca tcttgaacat
    32341 taaaggctta gcgctgaaaa tgtatggcca agaagaaaaa acccttgaaa tcgatattac
    32401 cggacctgct acagtaactg ctggcgatat tatcgttgat agtgatgtag aaattcttaa
    32461 caaagatatg tacatctgta gtgtctctga aggagctacg ttccatgctc gcttaacagt
    32521 gaaacctgga cgtggttatg ttcaagcaga tgaaaataaa aaagaagata tgccaattgg
    32581 cgttcttcca gtcgattcaa tctacacacc tgttcgtcgt gtgaactatc aagttgagaa
    32641 cacacgtgtt ggacaccgtg aagattttga taaattgacg atggagattt ggacagatgg
    32701 ttcaattgaa ccgttggaag caatgagttt gtctgcgaaa atcatgactg aacatttgga
    32761 tatttttgtg aacttaactg atgaagcaaa aaatgctgaa atcatggtcg aaaaagaaga
    32821 aacacaaaaa gaaaaaatgt tggaaatgac gatcgaagag ttagacttgt ctgttcgctc
    32881 ttacaattgt ttgaaacgtg caggaatcaa tactgtacaa gaacttacaa ataaatctga
    32941 gcctgaaatg atcaaagtcc gtaacttagg acgtaaatca ttagaagaag ttaaagcaaa
    33001 attgcacgac ttaggtttag gtttacgtaa agaagattaa aaccttttac gaaggaggga
    33061 aaccaacgtg agttatcgta aactaggacg cacatctagc caacgtaaag cgatgttgcg
    33121 tgatttaaca actgatttat taatcaacga acgtattgtg actactgaag ctcgtgcgaa
    33181 agaagttcgc tcaactgcag aaaaaatgat cactttaggt aaacgcggag atttgcatgc
    33241 acgtcgtcaa gctgcggctt tcgtacgtaa tgaagttgcc agcgtgcgtg aagaaaatga
    33301 ggacatcgtc attgaatcag ctttacaaaa actgttcaac gatattgcac ctcgttacgc
    33361 agaacgtcaa ggcggataca cacgcatttt gaagacagaa ccaagacgcg gtgatggtgc
    33421 accaatggtt gtcttagaac ttgtctaaaa tcacactaat aacatcactt tatagatgat
    33481 tcttttagag tgttatgatg ttggaagata tttttccgag tctagctcag cgctgctcct
    33541 caagcgaata tcgcttgggg agtaagcact catcataaag tgttttttta taccttcaat
    33601 tacctgtcat gacagatatg tttgaaggca taaaaaaaca ttttaagcga gaaataattg
    33661 aaaattttgt ctttttttga tagtgttagt aggaacttta ggatgaagaa ggagtaatca
    33721 tggagccaat tattgaatta gaaaagataa attataaata ccagccagat gatcttcgcc
    33781 ccgcattgaa agatgtctcc tttacaattg ataaaggtga gtggatcgct attattggac
    33841 acaatggttc aggaaaatcg acacttgcca aaacgatcaa tggattgctt ctgccagaat
    33901 cagggatcgt gaaagtggga aatcaaatat tggatgaaga aaatatttgg acgatccgac
    33961 agatggttgg aatggttttt caaaatccag acaatcagtt tgttggttcg acagtagaag
    34021 atgacgttgc ttttggtctt gaaaatcaag ggattcccag agaagaaatg ctagtgcgtg
    34081 tgaaagatgc acttgagaaa gttagaatgg ctgagtttgc ttcacgcgag cctgctcgtt
    34141 tatcaggagg gcaaaaacaa cgtgttgcca ttgccggcgt ggttgcctta cgacctgata
    34201 tcattatttt agatgaagca accagtatgc tagatccaga aggtcgtgaa gaagtgatct
    34261 ctacgatcaa gaagatcaaa gaagaaagtc aattgaccgt tatatctatc actcatgata
    34321 ttgatgaagc agctaatgcc aatcggatac tagtgatgag acaaggcgaa cttgtccgtg
    34381 aaggtacacc aaaagaaatt ttttctgctg gtcctgaact gatcgactta ggtctagatc
    34441 taccatttcc tgaaaaactg aagagcgcac tgaaagaacg tggggttgat gtgcccagtg
    34501 aatatatgac agaagaaagg atggtggatt ggttatggac atccgttttg aacaagtaga
    34561 cttcacttat cagccgaata caccatttga acaaagagcc ttatttgaca tcaatatgac
    34621 gatcaaagaa aacagttata ctgcattagt agggcatacc ggaagcggga aatcaacctt
    34681 acttcagcat ttgaatgcgc ttgttaagcc aacaagcggt acagtccata ttggagaacg
    34741 agatattcag ccggatacgg ataataagaa tttaaagccc atccgaaaaa aagttggcat
    34801 cgtctttcag tttccagaag cacagctatt cgaagaaacg gtagcaaaag acattgcttt
    34861 cggtcctaaa aactttggtg tcagcgaaga agaagcacta gtcctagcaa aagaaacatt
    34921 agaacaagtt gggctggatg aaagctattt ggaacgttcg ccatttgaac tttcaggagg
    34981 gcaaatgcgt agagtagcaa tcgctggtgt gcttgccatg aggccggaag tacttgtatt
    35041 ggatgaacca acggcaggac ttgatcccca gggtagaaaa gaaatgatgg agatgttttg
    35101 gcgtcttcat aaggaacagc agatcactat tgtattagtt acgcatttaa tggatgatgt
    35161 agccaatttt gcagattatg tctatgtgtt ggaaaaggga cgaatcgtta atagcgggga
    35221 gccgcaagag gtctttcaaa acattgaatg gctaaaagaa aaacaactag gcgtacctac
    35281 tgccaccgag tttgcagaag aattaatggc taaagggatg aactttgcta ccttgccttt
    35341 gacagctgaa gaattagcag acgctatcgt ccattatgct ggaggaaatg cccatgatga
    35401 ataaattgat atttggtcgt tatatgcctg gagattcctt gatccatcga ttggatcccc
    35461 gagctaaatt attagccagt ttttatttta tcggaatcat ttttttagca aataattggg
    35521 aaacgtacct tttgttggca tttttcacat tactttctgt cttcttgtca aaagtagatt
    35581 tgaaattttt tatccgaggc attcgcccat tgatctggct aatcctcttc actgtagctt
    35641 tgcaggtctt gtttacaagt ggcggagaaa cttattggag ttggggtgtt ttccatatca
    35701 ctgaatttgg tgtgatgaac ggcctcttta ttttctgtcg attcgtgttg atcattttta
    35761 tgtcgactct actcacgctt actacaccgc ctttggaatt atcggatgcg attgagtact
    35821 tgcttcgtcc attgaaagcc gtacggttcc cagtccacga aatttcattg atgctgtcga
    35881 ttgctcttcg ttttgtgcct acattgatgg acgaaacaga aaagatcatg aacgctcagc
    35941 gcgctcgcgg tgttgatttt ggtgaaggaa acctagttca gaaaatgaaa gcagtcattc
    36001 ctttgctgat tcctttattt gtcagcagct tcaaccgtgc ggaagatctt gcgactgcta
    36061 tggaagcaag aggctacaaa ggtggggaag gaagaacgaa atatcgtgta ttgcattggc
    36121 ataatagaga cacgatggtt atcattgctt ttgtattatt aactgtgcta ctgatctttt
    36181 tgagaggata aacgttcagt gggccgctat ctattctttt cttgctgatc tgtttgcaag
    36241 aaaggatgcg agcagtgtat gacaagaaat attagggtct gtggcagact aaggtcacag
    36301 gcctctttct tttatctgaa aagaaagaac gaaaaaattt tataaaaaaa tcattattcc
    36361 agataacctt ttaggaacaa aggagagaaa aacatggtac gttacaaagc aatcattgca
    36421 tacgacggaa caaattttaa tggattccaa aaacagccaa atgggcgtac tgttcaagaa
    36481 gaagtagaaa aaacattaca aaaaatggca aatggaaaag aaatcacggt ctttggttcg
    36541 ggaaggacag atgccggagt acatgcgatt gggcaagtaa tccattttga ttatccagag
    36601 gagcgtccgt tagaacgaat gcgtttcgcg ctagataccc agtcaccaga ggatattgct
    36661 gtaagacaag tagaaattgt ttcagaagac ttccacgcaa gatatttggt caaagaaaag
    36721 acgtatcaat ttcgagtgga tatagggaaa ccacgcagtc cttttcgacg ccattatgca
    36781 agttattttc cttaccctct tgatttggaa aaaattcagc gggcgcttcc tgatttatta
    36841 gggactcacg atttcacctc tttttgtgca tcaggaagct cgatagagga taaagtacgt
    36901 acgatctacg aggctaagat ggaggtaaat gaaacaaaag acgaattgtt gtttacattt
    36961 aggggaaatg gcttcttgta taagatgata cggatcttag taggaacgct tttgaaaatt
    37021 ggcaatggcc gattgcctga agatagtatc ccagatatta ttgcgaaaaa agatcgtaat
    37081 gcagcaggac ctacagcaca tcctgaagga ctttatttgt atgaagtgaa atatgaataa
    37141 gaggttgtga cagaagtggt cagcttcaag aaataagaag gaatttacga aaattacttc
    37201 tcaaaatttt gcaaaatttc ggcttatttt cgaaggagct acttctggaa caccgtgaat
    37261 cagaggttgt gaaaaagctg ccctgcatca agtaataaga acaaccaaac aaaaatagct
    37321 cgtcatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt
    37381 aaggaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa acggaaaaat
    37441 agttcctcat attttgccgt ttgttgagct aataccgaag gtcagctgat tgaacaccgt
    37501 ttaagaaaga ggtcgtgagc ctgtcattta actctgagtc acaagtaaca atttcaaaaa
    37561 acagcttctc atctttgatg aatatcgtga cttgtggacg aggcgttgat aagtgaatca
    37621 tctttttgtt acctattttt tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc
    37681 ttaatgagga cagtcggaac aggtttgtca tttttatatt taaatggcac aaatttttct
    37741 aattatttgt catttagcgc tgaaaatttt tgctatttat ctaaaaaagt gataactact
    37801 taagatgaag cagaatattt acgccatacg gcatacaacc gcgaaaaata taaaaaaaag
    37861 aaaacgattg aacgcgtttt tgcagtcgca aaggaaaagc atggaatgcg ctacatcaag
    37921 tatcgagact tggaaaacaa tcaggtgtat acgctgattg tttttgctgc aatgaatctg
    37981 aaaaagttgt ccacttggca gttggaacat atgtcttcat atcttttatt atacaaaaat
    38041 agcaaaagca ccagtctaca ctcttagtaa agaggaaact gatccttttg tctacattct
    38101 taaagtactt aaactaagta ctttatttta ctctttagga ttgatacagt agcatctatc
    38161 cctttattaa taatctttta tttttttaaa taaagatgtt gtctccatct tcaggcacta
    38221 aaactttttc ggaaattcct ttttctttat tgaaagactt taattcttct cttgacagtc
    38281 cccaatggtt tacagcttcc atatgaacgg caataattgt ggaatctggt attgctttat
    38341 gcaattcata taaatcgtct ttccccataa taagagattc tccaactaaa aaatgattat
    38401 ctccagcatt tgctactatg atttctggat tatactgatc aatagtttgt ttaacagctt
    38461 cataccaaac tgtatctcct gtaaagtata gtgtcttctc agtcgaatgt ttgaaaacaa
    38521 taccgcaaac attacctaca taattcaaga tttctcctct gccatgtttg ccttttgttt
    38581 taaccaattg tatagattca aaaactgtgt cttctcgtag tacctccaca tttttaaaac
    38641 catatagtcg aatttcattc gcatcatttt catcttgcac aaataatttt atttctttag
    38701 gtaggacctc tttcgctata tcgtcccaat gatctagatg taaatgtgtt acaataacag
    38761 catcaatacc gttgataata tcttcgactg gcattggcaa atcaactaga ggattcattt
    38821 gatcttttct tggtgatgga aaaggacctt tcgaaataaa tggtggaagt gttccttttt
    38881 ccgctaacat tggatcaatt aaaaattttt tacctccata gtctaagata gctgttgcat
    38941 tacgaatgtg atgtgtattc ataaacgttg tctccttttt gttgatatga aatattttta
    39001 gtataattca attccataca ataaataaac cgaacaaaaa aagtctttta ctatttttac
    39061 ttaatttatg aaaggttgtt taccatgtta gaccaaacag atataaatat cattgaggaa
    39121 ctatcaaaaa atagtcgaat cacaatgaaa gaacttggag aaaaagtaca tttgactgga
    39181 cccgctgtct cggcgagagt gacaaaacta gaggaaagtg gagttattga aaattataca
    39241 gtaaaattaa atatggaaaa gctagggttt tcgattcacg cttttttaat tataattacg
    39301 caaaatttaa atcataaacc ctatttatca tttattaaaa atcatgaaaa attcttgatt
    39361 agaaattata agattagtgg agggggctgt tatcttttgg aatgtagatt tacttcaaaa
    39421 aaatcaatga acagcttttt ggaacagctt aatgaatatg cgaattataa attatctatt
    39481 gttatagatt aataataaaa ctagcagatg gtaaaaatac taatatgcga gacacacatt
    39541 caaccataac tatataactt atcaatttaa aactaaactg atggaaacaa gtaaggcaaa
    39601 aacatttata ttttgcccta cttatttttt taattaaatt cattgatgat tcatatttag
    39661 aaaattctat gaaggccaaa tgagtatcct gtcacttttt tagataaata gcaaaaattt
    39721 tcagcgttaa atgacaaata attagaaata tttgtgccat ttaaatataa aaatgacacc
    39781 aagtatatca tgaacgcaca aaaagaccaa cagggtatac cctgttggtc taaa
//
LOCUS       DAZAFF010000014        39288 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000014 DAZAFF010000000
VERSION     DAZAFF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.014"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..765
                     /locus_tag="ACG9BB_000748"
     CDS             64..765
                     /locus_tag="ACG9BB_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFN2836717.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            762..1883
                     /locus_tag="ACG9BB_000749"
     CDS             762..1883
                     /locus_tag="ACG9BB_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFN2836718.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1898..3130
                     /gene="pepT"
                     /locus_tag="ACG9BB_000750"
     CDS             1898..3130
                     /gene="pepT"
                     /locus_tag="ACG9BB_000750"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFN2836719.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3262..4170
                     /locus_tag="ACG9BB_000751"
     CDS             3262..4170
                     /locus_tag="ACG9BB_000751"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN2836720.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4205..4477
                     /locus_tag="ACG9BB_000752"
     CDS             4205..4477
                     /locus_tag="ACG9BB_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFN2836721.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4488..5534
                     /locus_tag="ACG9BB_000753"
     CDS             4488..5534
                     /locus_tag="ACG9BB_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFN2836722.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5785..8394
                     /gene="clpB"
                     /locus_tag="ACG9BB_000754"
     CDS             5785..8394
                     /gene="clpB"
                     /locus_tag="ACG9BB_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFN2836723.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8545..9231)
                     /locus_tag="ACG9BB_000755"
     CDS             complement(8545..9231)
                     /locus_tag="ACG9BB_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836724.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9209..9703)
                     /locus_tag="ACG9BB_000756"
     CDS             complement(9209..9703)
                     /locus_tag="ACG9BB_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFN2836725.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9723..10943)
                     /locus_tag="ACG9BB_000757"
     CDS             complement(9723..10943)
                     /locus_tag="ACG9BB_000757"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2836726.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11090..12925
                     /gene="lepA"
                     /locus_tag="ACG9BB_000758"
     CDS             11090..12925
                     /gene="lepA"
                     /locus_tag="ACG9BB_000758"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFN2836727.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13121..14032
                     /locus_tag="ACG9BB_000759"
     CDS             13121..14032
                     /locus_tag="ACG9BB_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2836728.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            15043..15279
                     /locus_tag="ACG9BB_000760"
     CDS             15043..15279
                     /locus_tag="ACG9BB_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836729.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            15392..15634
                     /locus_tag="ACG9BB_000761"
                     /pseudo
     CDS             15392..15634
                     /locus_tag="ACG9BB_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15724..15963
                     /locus_tag="ACG9BB_000762"
     CDS             15724..15963
                     /locus_tag="ACG9BB_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836730.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            16110..20393
                     /locus_tag="ACG9BB_000763"
     CDS             16110..20393
                     /locus_tag="ACG9BB_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFN2836731.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            20492..20857
                     /locus_tag="ACG9BB_000764"
     CDS             20492..20857
                     /locus_tag="ACG9BB_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836732.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            20859..21311
                     /locus_tag="ACG9BB_000765"
     CDS             20859..21311
                     /locus_tag="ACG9BB_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836733.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            21760..23235
                     /locus_tag="ACG9BB_000766"
     CDS             21760..23235
                     /locus_tag="ACG9BB_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFN2836734.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            23258..23569
                     /locus_tag="ACG9BB_000767"
     CDS             23258..23569
                     /locus_tag="ACG9BB_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836735.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            23929..25185
                     /locus_tag="ACG9BB_000768"
     CDS             23929..25185
                     /locus_tag="ACG9BB_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN2836736.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            25182..25727
                     /locus_tag="ACG9BB_000769"
     CDS             25182..25727
                     /locus_tag="ACG9BB_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836737.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            25728..26228
                     /locus_tag="ACG9BB_000770"
     CDS             25728..26228
                     /locus_tag="ACG9BB_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836738.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            26587..26865
                     /locus_tag="ACG9BB_000771"
     CDS             26587..26865
                     /locus_tag="ACG9BB_000771"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836739.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            26885..27229
                     /locus_tag="ACG9BB_000772"
     CDS             26885..27229
                     /locus_tag="ACG9BB_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836740.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            27248..28159
                     /locus_tag="ACG9BB_000773"
     CDS             27248..28159
                     /locus_tag="ACG9BB_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN2836741.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            28238..29230
                     /locus_tag="ACG9BB_000774"
     CDS             28238..29230
                     /locus_tag="ACG9BB_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN2836742.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            29227..29445
                     /locus_tag="ACG9BB_000775"
     CDS             29227..29445
                     /locus_tag="ACG9BB_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFN2836743.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            29458..29868
                     /locus_tag="ACG9BB_000776"
     CDS             29458..29868
                     /locus_tag="ACG9BB_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFN2836744.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            29920..32448
                     /locus_tag="ACG9BB_000777"
     CDS             29920..32448
                     /locus_tag="ACG9BB_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN2836745.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            32464..34674
                     /locus_tag="ACG9BB_000778"
     CDS             32464..34674
                     /locus_tag="ACG9BB_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN2836746.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            34682..35773
                     /locus_tag="ACG9BB_000779"
     CDS             34682..35773
                     /locus_tag="ACG9BB_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN2836747.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            35794..36336
                     /locus_tag="ACG9BB_000780"
     CDS             35794..36336
                     /locus_tag="ACG9BB_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFN2836748.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            36531..36788
                     /locus_tag="ACG9BB_000781"
     CDS             36531..36788
                     /locus_tag="ACG9BB_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836749.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            36802..37134
                     /locus_tag="ACG9BB_000782"
     CDS             36802..37134
                     /locus_tag="ACG9BB_000782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836750.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            37179..37727
                     /locus_tag="ACG9BB_000783"
     CDS             37179..37727
                     /locus_tag="ACG9BB_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFN2836751.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            37734..38186
                     /locus_tag="ACG9BB_000784"
     CDS             37734..38186
                     /locus_tag="ACG9BB_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836752.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            38283..38624
                     /locus_tag="ACG9BB_000785"
     CDS             38283..38624
                     /locus_tag="ACG9BB_000785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836753.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            38992..39183
                     /locus_tag="ACG9BB_000786"
                     /pseudo
     CDS             38992..39183
                     /locus_tag="ACG9BB_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT        13377 a         6117 c         8304 g        11490 t
ORIGIN      
        1 ttttattcct agtctcctta ttttatttgg aaaatgctat aattatctag aaggagtgaa
       61 gctatgaacc atttagattt atctcagcgc ttagccgcag tgggtgactt tgtccctgaa
      121 tccgctcgtt tagcagacat tggatcagat cacgcttact tacccgttgc attgatatta
      181 agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc aaggtcctta tgaatcagca
      241 cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca ccgtacgcct tgcaaatggt
      301 ctagatgcta tccagccaca agatggtatt acagctgtca cgattgcggg tatgggaggt
      361 tcactgatcc gagatatcct agaagctggc aagttgaatc aacgcttaag cggacaagaa
      421 cgtttgatcc ttcagccaaa catcggagag cgtacattac gatcttggct gcaagaaaat
      481 cagtataaaa tccaatcaga aaaaatactt gaagaaaata aaaaaatata tgaaatcatt
      541 gtagccgaaa aagaacaaaa accagttact tacaatgaaa tggagctgat gttcgggcca
      601 tttctgcttg aagagaaaag tgagatcttc aaatcaaaat ggacaagaga gcttcaacaa
      661 agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc aagaggcacg tcaaaaacaa
      721 atcgagcagg aaatccaaat gattgaggag gttttaaagc catgacaatc agcggacgca
      781 ctttcatcca aaagtttgaa agctattgtc ctcagtggct agctgaagaa ggagacccag
      841 tcggactgca tatcggaaca ttggataagc caatccaacg agtaatgatg acattagacg
      901 ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat cgatttgctg attgctaaac
      961 atcctccaat ctttcgtcct gtaaaacggc tggttactga tcagccacaa gaaaaaatgt
     1021 atgcagattt gttgaaacat gatattgccg tctatgcagc acacacgaac atggatatta
     1081 tttgggatgg actgaatgac tggttttgtg aacttctcgg tattgaagtg gagagttatt
     1141 tagtaaagac tcatgagatt cattataaaa aactggccgt ttatgtacct gtggatcatg
     1201 cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg tacacaaggt gattataccg
     1261 gtacaagttt tacctcgatt ggacacggtc gatttactcc ggaagctggt gcacaaccag
     1321 cgattgggaa agtagggaag acagaacaag ttcaagaggc aaaagttgag gttattttgc
     1381 cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc agctcatcct tatgaggaac
     1441 ctgcttatga tcttttcgct atagatgaac cagtcgaaat gtttgggtta ggacgagtag
     1501 gtgaattgcc gcaagaaatt tctatagagg catttgtaga acaagtaaaa gaagcgttcc
     1561 aactagatgg actgagaata gtccagccaa aaaatgcaaa atcctctgtg aagcgcattg
     1621 cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc aatcgcccaa agagcagatg
     1681 tctatatcac gggagatatc tattatcata cggctcatga tatgcaaagc gctgggttga
     1741 ttgcaattga tcccggtcat tatattgaat cgttatgcaa gcaacgattt atcgagaaat
     1801 ttgaatcttg gaaacaagaa gaaaattggg atattaactt tttcgtttct gaaacaaata
     1861 caaacccatt tcaatttaat taaaaggaga gaatactatg tacgaaaatc ttttgccacg
     1921 ttttttacga tatgtcaaaa cagaaacacg ctccgatgca acaagcaaaa cgactccatc
     1981 gactcagaca caagttgctt ttgcgcaaga attgaaaaaa gaattagaag agttaggctt
     2041 agctgatgtt ttctataatg aagataacgg attcgttatg ggaacattac caagcaatgt
     2101 tgatcatgaa gtgcgttcca ttggatttat ttcccacatg gatacagcag attttaatgc
     2161 agtaggagtc aatccacaaa tcattgaaaa ctatgatggc gagtctacga tcgtactaga
     2221 tgcggaagaa cgctatacat tgaatacaaa agattttcca aatttgaaaa attatgaagg
     2281 ccacacattg atcacaacag atggcagtac attactggga gcagatgaca aatcagggat
     2341 tgcggaaatc atgactgcaa tggagatctt gatcaataat ccttctatcc ctcacggtac
     2401 catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc ggagcggata aattcgatgt
     2461 tgagcaattc gatgtagatt ttgcttatac agtcgatggt ggtcctttag gtgagttgca
     2521 gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc caagggaaaa atgtacatcc
     2581 agggacggca aaagatacaa tgatcaatgc tctacagtta gcaatcgatt ttcataatca
     2641 gcttccagcc gatgaagttc cagaaaaaac ggaaggatac gaaggtttct tccatttgat
     2701 ggcattggat gggaatcctg aagaagcttc tatgagttat atcattcgcg atcatgatcg
     2761 tgaaaaattc gaagcacgca aagcacaaat cacacatatc caagaagagt tgaatcagcg
     2821 atttgatcaa gaacgaatca aagtagatat gtatgatcaa tattacaaca tgaaagaaat
     2881 cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa gcaatgatcg agcttggtat
     2941 ctctccagtg atcgaaccag ttcgtggagg aacagatggt tcaaaaatct cttacatggg
     3001 tattccgacg ccaaatctat ttgccggagg agaaaatatg cacggacgat tcgaatttgt
     3061 ttctttacaa gtaatggaaa aagcaacaga cgtgattgta aaaattgcag aattgaacac
     3121 aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg ttcgtcccaa tgatggaatg
     3181 tcatctgtct aaggctaaag ggcaggacaa tggaattggt tgtcccgccc tcttttttga
     3241 agagtaaata ggaggtaaag aatggaaaag ctgctagttt tttataacaa aagttcaggg
     3301 aaagatgagg gtgagaaatt agcacgctgg tttcaagatt atgtaaaaga aaagcagcca
     3361 gaattatatg tagaactagc tgaaactggc ccatcgattg atgatgcgga attagtcaag
     3421 aaagcagaag ccatagaagc tgatacttta gtaattatag gtggagacgg cacgattcat
     3481 catatcgtcc aagcgttcca aaaaacatta tctcaatacc atgtcggatt acttccaggt
     3541 ggaactgtca acaaccttgc acgtgttctt gagattcccc tcaaaaaaga agaagctgct
     3601 gacgtgatcc tggaaggcaa tgtacggaaa atagactatg gtcaagtcaa tgatgaagtc
     3661 attatctcta cactgacaat cggaatctta gcagatacag cagtttggat cagtcaagaa
     3721 gaaaaacagc gttacggctc gtggatcttt atcaagaagt tttttaagtt attgctgaag
     3781 aagaaaaaat atcatttgga tttagaaaca gacgaggagc attggaaagg aaaatcccaa
     3841 ctgttgacag tcacgatgtc taactccgtg ggcggtttta caaattttga cgactcagcg
     3901 accctagatg atggaaaatt tcatgtaacg atcgtacctt ccttagatat tttccgattt
     3961 attttttatt tgcctagaat catgagaggc aaattctata cgatccccgg cattacttat
     4021 ttcacagcca aacgcatgaa aatccaagcg caggaaaaat ctgtgggcac tcgaacggat
     4081 ggagacacga cagatgatct tccagttgaa ttatctgttg tatcaaaagg attaacagtt
     4141 tattcaccaa agaaagaaga aaaagcttag aaatagtagt taacagaaag ttggaagaag
     4201 agtaatggat gtattgttac acaatccttt gtggttttac cttttcattg tgaattttta
     4261 cttattttgt ctgatggggt atgataaaca tcaggctaga actagcggct ggcgtgttcc
     4321 tgagaataat ttgatatttg tcagcctgtt aggcggtggt ttgggaggat tgatctctcg
     4381 aaaagttttc catcataaaa ctcggaaaaa gaagtttact ataggattcg ctgcaggtgt
     4441 acttgttgat ttgattctca tttatttttt ccattaagga gcaggagatg tcgtctaaag
     4501 gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat tgtgattttt gccattattt
     4561 attatttaat caatcaatca tttgcagata tctttgccga attgctgtct accagtgttg
     4621 ttgttttggc agcggctgtt atattaggga cagtttacca aatcgctgaa gggcgctcaa
     4681 tcaaagaaat tgctgaacct ttcgcgccga attttacaac gaaagatggg ttcttgacct
     4741 ctagctatat tgcgttttac cgaatcgttt cgtttggtac aggaacgctg atctcggaga
     4801 tttattttta taacaaaaaa ggaatgaagc tatcccaggg agcaggggtg acagcactcc
     4861 atatgattat gtacaaactg gcagtgatca cttatgcatt agctggcttg gtcatccaat
     4921 tctctctttt ttattctaat ggtccatcaa tgatctggtt tatcatcgct ggtatccttt
     4981 tgacgatcgt gattgttgcc tttttattgg cgatgtccgt cagcttgaat gttcaaatat
     5041 tttttgtcag cttatcgaat aagttattta aaagtgagcg tttgcggaaa tgggtggata
     5101 catgcaatca gcagatctac tctttaaggg aaacggtcca aaccattatc caagacagga
     5161 ctgcgctatt gcggatttat ctatggaata tgttaaaact tgtattttgg tatgtacttc
     5221 cttatttgat tctgattgaa aaccatccta aaatagattt tctactgacc atttcgttta
     5281 tcagctttgc agtagtgctt tcaggggtta ttcctacgcc tgctggtatt ggttcctttg
     5341 aatttgtcta tttgttatta ttcagaccag ttgttggaac agttgacgca gtttcttcgc
     5401 ttttactgta tcgtttcagt tcctttatct tgccatttct ctatggattt gtgtatgtga
     5461 ttgctgaacg gcgaagagtg atcaaagaag aaataataga agtaaaaaaa gagaataaag
     5521 aaaatcaagg gtaggtcttt tataagatct acccttttta tcaaatcaaa gaaaaacgtt
     5581 atggataaga taattttacg tttgtttggc tcttttttta tatacataca atcaattttg
     5641 tttttttaag atttatataa gtcgagaaaa aaagtcaaaa aactatgtgt atattttgac
     5701 caaaattgac caaagcgata taatagtttt tgtaaagatc aaagaaataa gtgatcttac
     5761 ctttaattag gagggtatac ctgtatgaat attgagaaaa tgactacgac gcttcaagaa
     5821 gcgattgctg aagctcaaca gattgcggtc actcgaaaac atcaagatat cgacattgcc
     5881 catgtatgga aaatcttttt acaatctaat cattttggtc gaaatttcta tacggatgcc
     5941 ggattggatg tcgaatcttt tgaacatgaa attgatcgtc tactggatga gtatccagtt
     6001 gtttctggtg gaaacgtcca atatggacaa aacctcagcc agaatttctt tcgattattg
     6061 aatgaagcag accaaatacg tgaaagcttc ggcgacgagt ttctttcaac ggaagtcgtt
     6121 attttagcgt tgatgaaatt aaaaaattat ccgttaactg tttatttgaa taaaaacgga
     6181 ttatctgaaa aagaattgcg taaaaacatc gaagagatga gaggaggaga aagagtgacc
     6241 tcacaaaacc aagaagaaca atacaaagca ttagaaaaat acggcgttga tttagtacag
     6301 caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg atgaagaaat ccgtgatgtc
     6361 atccgtattc tttcacgaaa aacaaaaaat aatcctgttc tgataggaga acccggtgta
     6421 gggaaaacgg caatcgttga agggcttgct caacgtatcg tacgcaaaga tgtgcctgaa
     6481 aatttaaaag ataaaacgat tttttcatta gatatgggtg cattgatcgc tggagcaaaa
     6541 ttccgtggtg aatttgaaga aagactaaaa gcagtcttga aagaagtcaa aaaaagtgat
     6601 ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg taggggcagg aaaaacagaa
     6661 ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg ctcgcggtga attacatttg
     6721 atcggtgcca caactttaga tgagtaccgt caatatatgg aaaaagacaa agcgttggaa
     6781 cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg aagatacgat cagtattctg
     6841 cgtggattaa aagaacgttt tgagatccac catggtgtta acatccatga caacgcatta
     6901 gtagcagcag ctactttatc tgaccgttat attactgatc ggttcttacc ggacaaagca
     6961 atcgatttga tcgatgaagc cagtgcaacg attcgggtag aaatgaactc tatgccaacg
     7021 gaattagatc aagttactag acgtttgatg cagttggaaa tcgaagaagc tgcactgaaa
     7081 aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt tgcaagaaga attagctgaa
     7141 cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa cagaaaaaga agaagtcaat
     7201 tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac acgaattaga agatgcagaa
     7261 aataactatg atttagaacg cgctgctgta ttgcgtcatg gtacgatacc tcaattagaa
     7321 aaagaattga aggaattaga agctaaagca aaagacagcg agattaagat ggttcaagag
     7381 tccgttaccg aaaatgagat tgctcaagtc gttggcagat taacgggtat ccccgtaacg
     7441 aaacttgtag aaggtgaacg agaaaaacta attaaattga atgaaacact tcataaacga
     7501 gtgattggtc aagatgaagc agttgatgcg gtcagtgacg cggtaattcg ttcacgggct
     7561 ggattgcaag acccaaatcg tcctctaggt tctttccttt tcctaggacc aactggtgtc
     7621 ggaaaaaccg aattagcgaa agcgttagct gagaacttgt ttgactcaga agatcatatg
     7681 gtacggatcg atatgagtga atatatggaa aaacatgctg tgtctcgtct agtcggagct
     7741 cctccaggct atgtaggtta cgaagaaggt ggtcaattaa cggaagctgt cagacgaaat
     7801 ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc atcccgatgt cttcaatatc
     7861 ttgttacaag tcctagatga cggacgcttg actgattcaa aagggcgagt agtagatttc
     7921 aaaaatacgg ttctgatcat gaccagcaat atcggttcac agttactgct ggaaggcgtg
     7981 actgctgatg gaacgattcc agaagcagtg gctgaacaag tcaacacgct attacgtgga
     8041 aacttcaaac cagaattctt gaaccgaatc gacgacacga ttttatttac accattgagc
     8101 ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc aattggcaca acgtctggaa
     8161 catcaagaaa tcctgctgac aatcagtgat gaagccaaaa cgtggattgc tgaaaatgct
     8221 tatgaaccag catatggtgc aagaccattg aaacgattta tcactaagga agtagaaaca
     8281 ccactagcta aagaaatcgt tgcaggacat gttatgccta aatcaaaagt aacgattact
     8341 ttacttgatg gacaacttca tttcaaaaca gaagagctag aagaaatcgt ctaatcttcc
     8401 attgagacta taaaagaaaa aaggggtctg acacaactag tgtcagacct ccttttttca
     8461 aataaacagt gttccagaag tagtttcttc gaaaaataag ccgaaatttc ttgaagctgc
     8521 ccgcttctgt cacaacctct tttattaggt atttttcact ttttctaatt tataactttc
     8581 aaagtaacgc tcatcttttt tactttgaga ccatgcaaac aatcccatta aggcagcacc
     8641 tatcgtattt gtcagcaagt cgcccatcgt atccatcaat gctgctcgtc cgacaaacag
     8701 tgtgccatca gaagcagcaa aacgttgaag attcatgcct aagaactggt cgcataagaa
     8761 ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac gcaaagccga ataagaggaa
     8821 caaccaagga ctggtttttg atatctcagc atctttcatc aagtaaccaa tcaatccata
     8881 gccaagagct gtcaggacca ttggactcac cgcatgtaag attttgtccc aaaaagagat
     8941 gatactgatc aaatgcatcc ctgtgcctaa aaagacagag atgaacaaga aaaaccagta
     9001 aaacaaaacg attgctgctg gtattttaag tttgaaaact ttcaaaatga cttgtggcat
     9061 gaaaatcaat gcaatcccta ataaagattc cacgataaat gtgattcctt gagaagtcga
     9121 atatttttgt gtgataaatt cataaaaatt atagcagaca acgaatacac caaaagcaag
     9181 taaagctatt tgcacctttt tgatttcttt aatcactgaa agtccctcca tctaaaaatc
     9241 tttttacttc tgaaagcaac tcaatttctg aagaaaccac ttttccgttc aatttgatca
     9301 aacctattgt atataagttc aggtagtgaa attgattttc agctgtttct tgtagtgcag
     9361 cgattttttt cgggtgggtt ccgccttgct gtcgagtatc agtatataag ccgatcactg
     9421 ggatgccttt cgcataagcc acaccgattt ccgatgctac cccggcatcg atcgtcagcc
     9481 catctagtaa agcaatcatt aaatcactag ccaacacttt ttctgtgtca gcaagagcaa
     9541 tcattcgact gtctgcataa gcagttttgt cattgattgc ttcattttct tgaggaagat
     9601 agatcgaaag ctcagaagag agctctctga tttctttaac taactgttgg ttatataaca
     9661 aatcactttt agcaaacatt ggtgcggcaa aataaatttt catctaaaca tcatcctttt
     9721 tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa tgatacgctc tagtgacttc
     9781 agtgtttctt catctgtttc taaccgttcg attcttgcca aataagaagc acggcgataa
     9841 ttcatcatca agcaagtcaa tgtttggata tccagtcgaa cggattttcc tataggttca
     9901 tttaagacac ggacttgtcc ctgttcatcc catgttaaag cgaagacacc attattccaa
     9961 ggagcgacag gatcttcaat gatgaaatga aaagcatcag ctgttccaac aaacggaaac
    10021 cgttgaagga attcttttac atcaacgatc cgagccataa aatatggttc gatctgctct
    10081 ttgatctgac tatcttcaat caaaaatgct aaaggctcat ttttataaat atctccatgt
    10141 acccagtaga tcatggaaaa atgtgcggaa ataaagttcc ataagccatt tcgagcttct
    10201 tgatcaagat agaacatttc ttttacgtga aatatttctt cagctaccca gtagaacaga
    10261 acacctcttg gttcatggtt gctatcgtag tagacagcgg ctgtgcgctc atcttcattt
    10321 tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga ataaagcacc atggttttgt
    10381 tgggcaaatt tgtcataaac ggaaaatacg tctggatgat caactggtaa tcgctcaacc
    10441 ataccactta ctgggatttg tttaggtagc tgtgtatctc tgattttaaa ggaaagttta
    10501 tcagacatga tttcccatcc tttacgccga tagtagggga tactgtaagg atacaaataa
    10561 gagatccatt gcttattctt gcgcatatta tccaatgcaa cgataattag atcttgcatc
    10621 aatccatggt tagcatattc tggataagtt ccaactccag tcacaccacc cattttataa
    10681 cgtgcaccgt gaatattgac ttcacaagga tagatagcga tttgagagat cagtttattt
    10741 tcgtggaacc atccgaacac tttcgatact tccaaaatcg gttttttgga acggataaaa
    10801 gctcgtttat tctcaaaccc gctttcctcg atgtctgctt cagtcacttg gaacacatag
    10861 gaaagcaatt cattgaattg atcgatatgt tcttgagtga ccggttttaa ttcaagattt
    10921 tctctaaaat ctttttgatc cattttttca tctccattta ttagcttgat aacagtatag
    10981 caaaattcta caaaaaaaac ttctatagct gactaattaa aaactcttta aattactgct
    11041 tttcgttggt ataatggaaa gtgctgatta tatgaaaaga ggagacgcta tggatataaa
    11101 gaaaatgaaa gaacgccaag agcagattcg taatttttcg atcatcgctc atatcgacca
    11161 tgggaaatct acattggccg atcgtatttt agaaatgacg cacactgtga cgtctcgcga
    11221 aatgcaagat cagctgttag actcgatgga tttggaacgt gaacgtggaa tcacgattaa
    11281 attaaatgct gtagaactca attacacagc gaaaaatgga gaaacatata tctttcactt
    11341 gattgacacc ccagggcacg tcgacttcac ctatgaagtc tctcgaagcc ttgctgcctg
    11401 tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc gaagcacaaa cattggccaa
    11461 tgtttattta gcgttagaca atgatttgga gatcctgcca gtcatcaata aaatcgacct
    11521 acccgcagca gatcccgaac gtgtccgtca agaaatcgaa gacgtaatcg gaatcgatgc
    11581 tagtgaagct gtattagcca gtgccaaatc agggattggg attgaagata ttctcgagca
    11641 gatcgtggaa tatgttcctg caccaagtgg agacttagat gcaccgctaa aagctttgat
    11701 ctttgactct atttatgata gttaccgcgg tgttgttcta aatgtccgga tcacggacgg
    11761 tatggtcaaa cccggagaca aaatcaaact gatgagtaat ggcaaaacat ttgatgttac
    11821 agaggtcggg gtcttctcgc caaaagcagt tgctagagac tttttgatgg tcggtgatgt
    11881 cggttatatc acagcaagta tcaaaactgt ccaagataca cgagtagggg atacagtaac
    11941 gttagcagac aatcctgccg ctgaagcttt accaggttac cgaaaaatga atccgatggt
    12001 ttattgtggt ctttatccaa ttgatacttc tcgttacaac gatttgcggg aagctttaga
    12061 aaaactgcaa ttgaatgatg ctgctttaca attcgaacca gaaacctctc aagcattagg
    12121 ttttgggttc cgctgtggat tcctcggact tcttcatatg gatgtcgtcc aagaacgctt
    12181 ggaacgggaa ttcaatctag aattgatcac gactgcacca tccgttattt accatgttaa
    12241 taaaacggat ggtacgactg ctacagtaga taacccggca gattttcctg aacctgtaac
    12301 gattcaggat gtagaagaac cattcgtcaa agcacagatc atggtcccaa atgattttgt
    12361 cggtgcagta atggaacttt ctcaacgaaa acggggagaa ttcattacga tggattattt
    12421 agatgattac cgagtaaatg tcgtctataa tattccgctt tctgaaattg tctttgactt
    12481 tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca ttggattatg aaatgtctgg
    12541 ttatcaaaaa agcaagttag tgaagatgga catcttgctg aatggagaaa aagtcgatgc
    12601 attaagcttt atcgttcacc gagactttgc ctatgaacgt ggaaaagcaa tcgttgaaaa
    12661 actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca atccaagcag ctattggaca
    12721 aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa aacgtcttgg ctaaatgtta
    12781 tggtggagat gtttctcgta aacgtaaact cttagagaaa caaaaagaag ggaagaagcg
    12841 gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc ttcatggccg ttctgaaaat
    12901 ggatgagcaa gatagtgtga aataacttat aatcaatcga aaaacctatt aaataaacct
    12961 ttttgagtag atgcaacact gaggattatc tgtataaaag attttaaact gtcatttggt
    13021 tatcacggcg atactaaaat taacaaaatt ggaatatttt tcttgaagat ttttagctgt
    13081 cagtatatga tgaaagcatc tcataggagg tgcttttttc atggttgata attcaagtgg
    13141 aagggttctt aaatctctaa gaaaagagaa aaaactatct caaaaaaagt tagctgattt
    13201 agcaggtatt tcacaaagta ctttagttaa atatgaaaaa gggtcaagaa aaatccctaa
    13261 agacgtagat aatactctaa gcaagattct gaatatagaa actctaataa aagatgagga
    13321 agataagatt gagattctca tcggtaaact aattgcatac agagatatga ataagttact
    13381 taacaaagag ttagctgata atataggaat tagtgaagta ttactcagtt atgtgttaaa
    13441 tagaaagaga aatccgtcaa aagaaatgca aaaaaaaatc gatatttttc tattaagtaa
    13501 tgaaaaagaa attttgaagg agattaatcg tgatagtgag atatttagtt tatcaaaaga
    13561 cgataaaatt gttatgggaa aaagaatacg agaagtccga aagaaccggg aagagacgct
    13621 tgaaaaattt ggtaaaaact ttaccatcta tacaggcaaa aatgttataa gtagatggga
    13681 aaaaggtatt aatattccag atattgaaaa actaatgaat attgcctatc ttggtaaggt
    13741 cactgttcct taccttatgt acggagaaga ttataagaat atacttccaa aagatgaaag
    13801 agttagcgat tttaaaaaga taaattcttt tagtatggga ttaaggatga ggaaaattag
    13861 gaaagattat tatctagaaa gggaggagtt tggaaagctt ttttctcctt caatttcaaa
    13921 atggtccata gataggtatg agaatggtag agatataccc aatactaatc gaattatcca
    13981 atatgcttat ataggaaatc tttctttaga atttttgatt tacgggatat aaaaatagtt
    14041 tacgtcgata cttattaagt tatcgacgtt ttttattata gaaaaaattt ataaaaggag
    14101 gtttcttgat atgataagaa acaaaaaggg tttgttgaaa gttgctttac tggcagcttt
    14161 tttctaagta actttttgac aagctcaatg gcaacacaag cggaagaagt aatacaagaa
    14221 ctaactgtta tgaataacag ctagataaaa tgggctatgt atgttatcca acaatttagt
    14281 aatcactaga agcctagcga aagttcgctt gataagttat agatgagtat tgatttgtag
    14341 tgtttccaaa tcaaaagggt acagagccac atgaaatatg ttgtcctctg cagcgtcagg
    14401 atgggaatcc ataccatgga gattcggaga tctgtgaggg aatacttacg tggttaatat
    14461 gtcagttgtg ttggacgtag taatggtcat agtgggtaga ttgactgaat tcttatatga
    14521 gaaaatgaaa ccaacaagaa gatagggtta tttatacaga attggggatc ttttctggaa
    14581 aagtaatcgg ataatgcgag attcgtacgg tcttttttaa atttctctaa aaaaaacggc
    14641 atcgaatggg ttgggtgatt gataagcaat gaatcataga ctgatataga ataaaaaatg
    14701 attctattta tctttttaca tctatcgttt tttcaagtag ccgcaaatta tatcagcatt
    14761 caacaatttt tgtatacaag ttctagtaaa aggatctgaa gaaaaagttg gttgttgtga
    14821 agataaaaaa agaatcaggt tttactgagt ttttcttcaa aatttgtgtt attaggttag
    14881 gacaaagata agaagaagta tgaagattta aaaaaggaat gaggaaaaga tagtaatttc
    14941 cgattattgg agttttttta tttataaaga agaagcgaat aagttgtgat tcctagattt
    15001 cgcgcgtgat taattaatga gcaaaaaatg cggaaagttt tagtgtttaa tacacgagga
    15061 caaagtttct taggtattga aggcacacgt gtaacggttg atgggtggtg tgattgtttc
    15121 gaggttggaa aacatgttga acttatggca tttactgagc taaaagataa gaacaacgtg
    15181 gaatttttcg agagtgatat tggttgggac gatcatgaag aagtttatgg aaaatttatt
    15241 ttgcggaagg gaaattctta tacgaatggg gaagcataag tagcgatttg ttagagatca
    15301 ttgataggat tgaaattgtc gggaatattt atgagaatct agagttattt gaggtgatag
    15361 aatgagtgat ggttttgaac aagtatcaat aattaaaaat caagttcggg agattcttag
    15421 aaaaaaaggc tatttggtag atagttattt tgaaggagat tacgaaacat gggtaggtgt
    15481 ctatgctcgt ccagagaata aacctaccta tttagatcct acgacgtcag aagatgccta
    15541 tttacaaaat aggtatcgag tagatggttt taagcaagac tttgcggagt ggtttgaatg
    15601 ggaaattgaa aacggagaag taaaagaaga ataagagtac ttgttgtatc aaaataagcc
    15661 agtagaacaa ccaaaccagt agctagtaat gacaataata ctgaggaatc ggatcattat
    15721 ggtatgattg atatttctct tccagaagat cttaagccag atcctgtaac aattggcaat
    15781 agtgtcatga tttttgattc attagatgaa gctatgtcat atggttggaa agaaattaag
    15841 gatgaaggta gtaattggta tggcatgaga tttggtgatg gtgaagttgc caaaaaagct
    15901 ggtattccaa ttgggaaatg gacagttgat ttccacgaag caggttcttt ttttgttctg
    15961 taattatagt gggagggtaa aatcattgat gaaatgtgtt cagataagag cgccagataa
    16021 aaatagaaga gacagttcac gtcgttatca cttttggtaa cggcgttttg ttttattata
    16081 taaaattctc aggaaaagga ggaattcttg tgtatgcaaa aacgaaaatg aattggttga
    16141 aagcactcgc attggtggct ttttctcaat tctgtttctt ggtgggaact gctcaagcac
    16201 atgcagaaga aacgacggct atacaaccta cgatggaagc cattgcccaa gtcgcttctg
    16261 aagtgacaga agtccaacca gcagaagccg gggtcccagc tggtgaagcc actgttgaac
    16321 aggcccctgt gacagaagcg ccaacggtca attccaaaac gactctcgat cagacgattg
    16381 gggaaacaca acacccaggg aatagtcctg gtacttcaca accagttgaa gaaactgcct
    16441 caattacaac ccctgcttca gaaacgtcca ctctagaagg agaaaaagct gccgaagcaa
    16501 cgccttctgt gcagccagag tcttctgaga aacaagcgga agaaaagaaa cgaatagaag
    16561 aaacagtgga acaaccagcg gctactgctg acaaagaagg aaaaaatgag gaaaacgttg
    16621 aaaaaacaat ctcttcaacc gcagctagtc aaaatcaacc aacgaaacca aacaaagaat
    16681 ctacggctag tattgaaaat caaaagaaaa aagagcagaa cattccaaaa acaggtacac
    16741 ctacactgtt agtaacagta ccaaccaatc gttcggttcg tgttcgccgt gcaaccaatg
    16801 cagctgtttt aggtggtcgg aaaattgatt ggggcgaaaa tattggtgtt acttctagtc
    16861 cgaacaatct aaacttcact ttaaattata tggttcgttt tgagattaat ggacaagtgg
    16921 cgttttgtat tgagcctggt gtctcagccg cagaaggtca agtttataca gctactgctt
    16981 tgaaaaacta tttgaaaaat tcaaatgtac ggaaaaaaac aagcttgatc tcttatttag
    17041 gatatgtcac aaataaagac aaatccgatg aacaatacat tgcggcacaa tttatgattt
    17101 gggaagcttt gggcacaaaa atcccaatca aaaatatccg tgttgattat gatcgtcgaa
    17161 aagcagaaat tcaacgtttg attaataact ttggtgaaaa agcaactttt aataatcaaa
    17221 cacatacggt aaaaattggc gaaacgttga aagtaaagaa taaaaatgat tttttgaaat
    17281 atgtgaaaga cattcaaaaa ccaaaaggtg tcaatgtatc tattcacggc aatgaagtat
    17341 ggattacagt cacaaaagag gcaccggata aagcaaaaat tcaattgaat aaaattaaag
    17401 ttcaaggtgc gccattagcc tatcgtcgtc caggagctca aacaattggt gtgttacatc
    17461 caacagaacc taatgcgtct gttattaatt tacaaattct aaaacaagga aacattcgtg
    17521 tgtttaaaga agatgcggaa actggtgtga aagcacaagg tgcagcttct ttagaaaatg
    17581 cggtctatgg tttgtatcaa gcagatggga aaaagattaa agaaattacc ttgaaaaaaa
    17641 ttaacggcaa agtacaggca gaaattaaag atcttgatcc tggcaaatat gttttaaaag
    17701 aaatcaaagc gccaaaaggt tatgtgcttt ctgatgaagc aattgaaatt gttgttgaac
    17761 ctggaaaaac tgtcaatgcg accgcaaaag atcaagtgat caaaggaacc attgatttag
    17821 tgaaagttgc taataaagat ttagtcgatt caacgaatcc agataacaaa ccaaaattag
    17881 ctggcattga aatcactttg acgtcaaaaa caactggcaa agtggtgaaa accgttgtga
    17941 cagacaatga tggttatgct acttttggta aaaataccgt tgtctttgat acgtacattc
    18001 tttctgaaac caaaggaaaa gaagggtata aactatttga accacttgaa gtcacaattt
    18061 ctgaaaatgg gcaaactttc cattatgttt tagaagataa agtaattgaa caacgtctta
    18121 aagtggtgaa agtggatcaa gaaacaggaa aagtgattcc acttgctaat acagaattca
    18181 agatttttga tactttagcc aatcgttatg tcacaatgga gattccaaat gatacagaaa
    18241 caacggatat ctttaaaacc aatgaaaaag gctttttcct aactaacggt acacttactt
    18301 atggaaaaga tcgctatcgt ttggaagaaa tcaaagcccc cgtaaattac gttttgaatc
    18361 aaaacccatt agtgttctcg attaccaatg atacagaacc gatcaaagtg atccattttg
    18421 cgaaccgtca agcacggaaa agtgtaaaat tattcaagta tgaagaatgg aacaagaaga
    18481 aaacgccgct cgcaggtgtg aactttacct tgtataacgg taaaaatcaa gcaattggtg
    18541 aatataccac tgatcaaaac ggggaaattt tagtgaaagg tctagttgcc gatgactatt
    18601 acttcttgga aaatgcccca ttagaaagtt tccaaccaaa tacagataaa caagcattca
    18661 cggttgcatt caaacaaaat gcgccagatg atggggaaac gttagtggtt tctgtacgta
    18721 attacttgat tccaccaaaa ctagaaacac aagcgtcaaa taaagccgat ggtgaaaaag
    18781 tggttgatcc attagaaacg atcgtcatta aagacagtgt ttcttatacc aacttgtttg
    18841 tcggaaaaga atacaccatt tcaggagtat tgatgaacaa ggcaacgaat gcgccaattt
    18901 tacaagatgg caaagaaatc acaagtacct tggcctttat tgcagacaaa gtcaatggca
    18961 caaaagacct tgaatttgtg gttaatgcta gtgttctgaa aggtcaaacg atcgttgttt
    19021 ttgaaaaact tttccgtgat ggcattgaag ttgcagcaca tgcggatatc aacgatgaag
    19081 cccaaagtgt gaaagtgaat aatccaaccg ttcataccac ggctgtcttt gaagacggat
    19141 tgacagtcgc agacccaaca ggattgttta ccttaaacga taccgttgaa attaccgaga
    19201 ttcttccagg aaaagcatac ttggtcaaag ggatcttaat ggataaagca accggtgaac
    19261 ccttgcttgt gcatggaaat acggtagaaa cgagtgttgg ctttattgcc aaagcagaaa
    19321 atgaaacggt gatcgtacca tttacctttg atttgacagg tataaatggt cgtgaaatcg
    19381 tcgtgtttga aagtcttttt agaaatgatg aagaactagc aaaccataaa gacatcaacg
    19441 acaaagggca aacggtacga gtaactaacc caagtgtacg tacaacggct acacataaac
    19501 ccactggctt gaaagaggtt aatccattaa cgaaagtcac cattacagac gctgtgaaat
    19561 accacgattt ggtcgttgga aaaacataca cagtcaacgg tgttctaatg gacaaagtaa
    19621 cggaaaaacc agtgctagta gatggaaaag aagtcacaag taccttaacg tttgtggcag
    19681 aaacaacgga tggtacagtc aatctagact ttattctaga cgcacgtgcg ttgcgaggca
    19741 aagaagtcgt ggtctttgaa gatctgttta gagaaggcgt attattggct tctcacgcag
    19801 atattaccga caatgaccaa accatcaaaa tcagtaatcc aaccattacg acgaaagcga
    19861 cgaataaaaa tggcggaaaa gagatcttgg ccttgccaaa tcaaactgtg cttgaatggg
    19921 tcaaagtaga cggtcttgcg attggtcaaa tctatcgctt aattgttcaa ggctacttaa
    19981 caccagacgg cacaccattt gaaggcactc atgcggaaaa agtctttgcg gccgataaag
    20041 aaatgatgga attcttattt gaattcttag tggacggtcg caatcttgca ggaaaaggct
    20101 tgagttttgc ggaatggctc caagcgaaaa cggaagatga gaaagaaacg tttgaagaag
    20161 tggcgaaaca caatgtagat ctaacaaaca aagatcaaac ggttcaaatt gtggaagcgc
    20221 caaaaccacc gcaaccagtg aaaccagaac aacgggttaa accagctact gttgtaccaa
    20281 tgttgccaaa aacaggtgaa caaacaagta gtatacttat ggttcttgga ttagtagtat
    20341 tagtcattgc tttaggtata acactttata aagtacgtaa agccaaagat tagtcatgag
    20401 gaacaagcgg ttaggatttt taaccgcttg tttgattgta atgtagaaat atttagaatt
    20461 tattttggtt tttaagagga ggaaaaactt tatggcagaa aaagcattgc gtatgaagaa
    20521 agagttttta ggtcaagtat ttttattggg ttcacgggaa cctaatccac caaaggaaat
    20581 taatggtgtg tctgggcgat atgttcaatt acaagcagag tgttcgcgag aagaacagcc
    20641 tgcttttttt gtttatctca cagctgaaga ggataaaaga ttacgattac cagaaaaaga
    20701 ggtgcgtatt tcatttgaag aagcttattt tgtttttcgt aaagaacgag tgaatagtcg
    20761 tgaaacaaat gatatgggac aaacagagcg tcgaacaact ggctatcaag atgttggtgt
    20821 ctttttaact ggtgtaaaaa ttggggaggt tttgtaagat gggattaaaa tttccaaata
    20881 attatgacat tacagaagca ttagatgaaa cttgtttagg agccgtacgc tttggcgcat
    20941 tagaagagtc tgatcgtgta tttgataatg aaacaggcga ttatacggga gaaattcgat
    21001 cacagcgagc acaattagtt tttggaaatc cagatgaacc aaagattgca cgacgagtta
    21061 aatttccagc agatgtagat ttgactggtt ttaaacgacg tgatcctgtg aaattaattg
    21121 atccagaata tcatgtggta tatattccag cacaagatgg caatcgtgaa tattcagatt
    21181 tagtgattta tgcgaaagga ttagagaaag tagaagctcc aacaaataat cctaatatga
    21241 aacaatcaca agaaaaaggt ttagatcaaa aacaaccaag tgttgattct aaggaaaaag
    21301 agaaaaaata aggttttttt gaaagaatag gaaggagtct tcctattctt tttatctaag
    21361 atagaaggaa ggggagattt tgaattcaga taaaaccaat caaatacttt gagagattgt
    21421 gcttgttatc aaaatatcca acaattgatt gagtattttg gagacaatcc aacgattttt
    21481 gtacaaacaa atgcatatat ttgtgcggtt attgggcagt gtatgttttt acgtgatctt
    21541 gttcgttttt cattaccgta ccagtggaaa caagatcatc aggaagtgat ttggattcgt
    21601 atagtacgaa tatgtaatat gtgtgcacac ccttataaaa atcgattaaa tctttttcga
    21661 ttatgttttt ttactaaatg tgatattcct gtattagttg ctttttgtca aaagttttta
    21721 gcacaggaaa agtaaaaaca gaaaggagga atgtgacaat tgaaagctca ttttttcaag
    21781 tatcgtggtc ggcgaattcg ctatttcaat cgttatttat ggttttgcta ttatggtttc
    21841 tttttgtttc catttttgat tctgttaggt tggtttagtt ggaaagtatt ttatccacgt
    21901 ttaagtacgt tttcgaattg gcaaatgtat cttattcctg gaataagtat tgtgttgtat
    21961 ttgttgctgg ttagtgggtt aattttaggg ttgatgcatt ggtcgcgact aaaagaagga
    22021 tattttgcct ctgtttattg gcgtcagtta cttgttcgaa tgttaattga caatcggttt
    22081 gtttatacaa agaaacaagt tagtgagaaa caaacaaggg aaaaaatgag attaccaaaa
    22141 gtttattttt atcaaaagaa acaagaatta gtggtttctt ttcctttaga tggtgggcgt
    22201 tttcacgaac gatttttaaa aatgggtcat ttgttatctg aagtattttt gcgtgattta
    22261 attcgtgaag aacgagaaaa agcccgaatc aattatgtat tcctttcaga ttctggtcgg
    22321 attcctattg atcaatgtat agcagaaaat ggacgtattc atttaatgaa aactttggat
    22381 tgggtatatg atcaatcacc aaatatgttg attgctggtg caatcggtgg tgggaaaacc
    22441 tatttattgt attctttaat tcaagcttgt ttaagtgttg ggacagtaga tatttgcgat
    22501 ggaaaagcgg cagatttagc agcattaggt gacgtcgatg tattcaaagg acatgtgttt
    22561 tacaaaataa atgaagaaat gattatttgt ttacgaaatg cattaaaaga aatgaatcaa
    22621 cggtatgcct atatgaaaac catgaaagat cctaaatatg aaccagggaa aaattatgct
    22681 tattatgggt taccaccgca ttttatcttt tttgatgaat gggcagcttt ttatggttcg
    22741 ttggatatgg aagcgagaaa tagagtcgat aaattgattc agccattagt attaaagggt
    22801 cgtcaagcag ggatgtacat tattttagca atgcaacgac cagatgcaga atattttcca
    22861 agtggtgttc gggataattt aacattccgt atttctgttg gtcgattgtc tcccattggt
    22921 tatttaatga cttttggaga agattttaaa aataagccat tttacaacaa agatcaacct
    22981 ggtcgtggat atgcttttcc aggatttgat gtacctgtag agttttttgc gccatttgtt
    23041 tacaaagatt atcactttat caaagaattt gagaaattta ctgaaatgat tgtctatgat
    23101 tttacagtaa aagaggatag tcaagatcaa acgatgggag atgataatca gttgttacaa
    23161 gaacaactag agcgccaaat gtatttagca aaagatccaa aaggaacaag tttttctgat
    23221 gcgcatattt tgtaatcaac taaggaggtg aagttaaatg gcagagagtt gttattatag
    23281 agtagctaca gcaatacgaa tgattaatcc ttctctttct tccagaacgt tttatgattg
    23341 gttgaatcgt attgaacaag taactgatta ccgctttttg cgcaaagaac gtgtttttac
    23401 aggaaaagtt attaatcaag tattgctaac gaaaaaagat atagaacgat tgactcgatt
    23461 gtatcattat cgagtagatt tagaagagga tttaactttg tcaatttatc gcgttttttc
    23521 tcctgaaaaa tatagtgaaa taacaaaatt agatcattta attttgtagt tattatctat
    23581 cgggagggct ctagacgtag tctggtagcc gcactctgcc tggcggtgcg cattaaggct
    23641 gcgaagcatg ggcctataat gcgcactcgc tagcagcgtg tgcagctcac aaagaggtcg
    23701 tgcgcttgcg cacggcctct ttgtgtaaag ccccccgata tataatatgg ggggcagaaa
    23761 taacataaca catagaaaaa agttttataa acccttttgt atcaagtgat tatctaaggt
    23821 cagcagttgt actacttttc ccaaaaaacg agggggacta gttgatatat cggtgtttct
    23881 aaagggttaa aaaaatcgat tttgttattt tataaaagga gcgagaaaat gacaccgatt
    23941 gagctaaaaa gctacagaaa gaagttaagt gcaattcata atataagtgg agataaaaag
    24001 ccgctatcac aagcaagatt tgcgcaattg atgggctgct caaaagatta cattagaaag
    24061 atggaacaag ggaaaagaaa aatcccagag gattttgaac aacgaatcaa ggaacgcttt
    24121 cgattgtata tgaaaagcgg aatagatctt cgagcaatga ttgattattt gcgtctttcc
    24181 ttttttggga cagactacaa aacagtgatt caacgtgtat tacgaatgga tctgacagaa
    24241 tttgaaacac gtccaacggg cttgtatctt tttgactgtc tttctgttcg tgggaatatc
    24301 tgggtgttct ggcattcaaa acgaactgta acagaaaatg tacttgtgca attttcagga
    24361 caaggttgcc gggaatatga ggaagtcatg cgtgaacaac aaacagattg gcgaacatgg
    24421 ctatattcca tttggaaaaa tcaaggaaag ttggaagctg gttatacacg tgtgcaatgt
    24481 agccgattcg atattgcgtt agatgaactt tggggattgg aaacggaaga acatttcgat
    24541 ctctttgaat tactagaaaa acaaaaagca ggtttattag aaatggactt taagagattc
    24601 cagaatattg gcggttgttt tttagatgat ggctattttc gaagtgaagg attatctttg
    24661 tattttggat cgcgaaagag tcctctcttt tttaattttt atgagaaacg ttttgaaatt
    24721 gcgaaccgag aaaaaatttc agaattagaa gcattaacga aatatggtat ttataatcga
    24781 tatgagttac ggttagccaa tgagaaagca acacaagcag tagaagcatt tattttaggt
    24841 gattcaccga aacgtttagg agagattggc gttggtttaa ttaatgcaaa acttattgtt
    24901 tgggatagac tggaaagtga tgcacggatt gttgataaac gatgggcaaa attatttcgt
    24961 tcgatggaag aattggattt tacagtaaag ccagaaaaat tctcattaga tcgttcagaa
    25021 cgctggtttt tgaaacaagt agctccgaca ttaaagttgt tacaagaagc agatcgtatt
    25081 ttaggaagag actttgtaca agaagggatt gataatgccg aactaaatga cgagcaacaa
    25141 aactatctag aatggatggg aacgtcagga gggaaaattg catgaatgca tatacgacaa
    25201 cagaatggct gcgcctgttg gatttaaagg ctgcaatcga agcattaaat gaaaagatgg
    25261 ttgatttgtc ttattttcgt tttcgagttc cttatattga acaagcggta aaagctggac
    25321 gttatcagga aaaagagaac tggcaagaaa ttgcacgctt attagaagtt cgtaagggat
    25381 acgaacaaga actcgaagag ttggaattta gccgaaggaa aggaaggcta gaattcattc
    25441 gcttttatcg attttcattg ccaatacctg cgattttagc ggttaaaaaa ggctgcgata
    25501 aaatgaagat atatgaaaac tgtgtggcag cacttagcag cgaaaaacct cttatggaag
    25561 aaatttcatt ggtgactgtc acttgggaaa tgagtcgcca gccaacagaa gaacagacat
    25621 atctttcttt agaagagatt gagatagaat tagaggaaat tggtcgctat gccacttgtt
    25681 ctacctattg tggatcggtc attagtattg ctggtgtgat tgtctaaatg aaaaaaatta
    25741 tgatttatgt tggtgcgtat tggtcacgtg atccaacagt attagaaaat ccagaggcgg
    25801 tacactattg cttgcgccaa ttattctatc tgtataaaga acgattagaa tcgttgattc
    25861 gtcagttacc atatactgac agacgtttag atgaacttct tctaaggtat ccagcaatgt
    25921 ataaacgacg aaaaaataga ttgttaccag aagagtatcc cattgaaaaa catgaacttg
    25981 aagggcgatt tgtggcttat ttctatgatg atgttcgtat gcgcttagta gaacaacgaa
    26041 tggaaataga aaatgatcgc tactatttta tcaattattg taagaagcgt aagtatcaag
    26101 taactgatga tttttatgat tgtattttac aagatgggga agtgattcta tccaaaattg
    26161 ctccaagttt tcgtgaactt gtgccgattg attttttgaa aaaatgtcat ttgcggattc
    26221 ttccgtaaaa aagagctata aaaggaaaga aaagtagaag ccaaatcggt ttctgctttt
    26281 ttattttttt taggaaggag aagatggtat gtcactggaa tttttaggag agccaattga
    26341 aaaggaagag ttcgtttcgc attatatgga tttgtttatt caggtgataa aaaactttgt
    26401 gcgattgatg aattttatcc acgcgaacaa gactatctag ccgctgaacg aataaaaatc
    26461 agtttattat gggaaaaatt cacagatgaa aataaccgac cgccagatta tcgctattta
    26521 tcagattgtt taacgaataa tgtgctttct aaagaatacc tatttcacaa acgtgagaaa
    26581 aatcaaatgt cggttgaaca tttttgtgat tgttatattg cattattgcg tagtcaacag
    26641 ttattaacag tagaacgata tggtgcagag tttggttatg tcttgcaagc tgagcgtcat
    26701 agagctacaa gatgctatga aaaagaagat caagtgattg atggctatga agaattatgt
    26761 attcgaaaca atcgctattt gcaacaacgt ttatatgaac aattgatgat ggatttttca
    26821 aatctctata gagggtatga atgttcgatg attcaagaaa ggtagaagaa aagggggaat
    26881 ttttatgtgg cgattattat ttttggtggt atgtggtatg gcattgttct atgtgtatcg
    26941 tgtctatctc tatccacgaa tggaacgatg gacaagttgg tatagagatc ctttaaaaca
    27001 acgaaaagga ttgaagcggt atcgttatga attacttgtt gaattttcgg atgggcgaca
    27061 agcacagtat tatttttttg caaactatcc aaaatacaac accagtgaac tattgattca
    27121 attattcaaa aataatccat atgttgggtt agaagaaaac ggacgggagt tcttttatcg
    27181 ttcagataat attagtgcgg cgtctatgaa caaaacacga attgcataaa aaattggagg
    27241 aataatcatg aaaaaaacaa cattagcagt cgtaacaaca ctaggaattt cattattttc
    27301 gtttcaatta gggagttcat tggtttatgc agatgaaact gtagaaacac ctaagtcaga
    27361 aattgtagaa acattgccag gggaaggatt accagatgtt gagacagagc taaatggagg
    27421 aacaacggaa aaaccagcag aacctgaaca attgcagtca gagaaacctg aaaaagagcc
    27481 agtgacacca gaaccagagt ttcctgaatc aactttaccg actgattcga tcgaagaaga
    27541 aggtgtgcat agttcaacaa caacggattc aagtatagtc acaactccgg aacaatcagg
    27601 aacaaccagt tcttctgaaa agaatactga ttcgtcaagt acggaacagc caacgatgtc
    27661 gtctaccagt gaagaacaaa aagaagaacc gaaacaacct gttgaaaaat cagaagcaat
    27721 tccaaaagaa aataaaccta ttgtttcagc aaaagaagtc acagtatcgg taaccccaag
    27781 cggtgaaatt acgacgaata cttctcaagg aatgagtgta ccgattgtca ctagtaatgt
    27841 agaggaattg acccatattc caacaccaac caccccatta aaagtagcag gtgatcaaac
    27901 aattgttggt gtaaaagatg gtattccatt aatccaagat agtgaaggga atttggtaaa
    27961 ggatttgtcg attcctgtaa agaaacttcc aagtggcaat attgaaatca aaacagcaga
    28021 tggaaaaaca aaagtcttac caaagactgg tgaagaaata catgttgcac tttctgttct
    28081 aggaggagtg ttgacaagta ttgctggatt tgttggatat aaaagaaaga gaaaaggaat
    28141 agaaaatgag ggtaattgaa tggactattt ttcagaaaaa caagcttatc gttgtcaatc
    28201 gtacgtacta gtaaaaacat actagaagga gtagcttatg aaaaaacgac aactacggcg
    28261 gaaagaaaaa gtttatacag agaaagtacc gaagcaaaag aaaatccatt caaaaaaagc
    28321 agtgaaatgg atttggtgtt tggttttatt tctttgtttt tcagggggct tggcattttt
    28381 tcaatccatg aaaacgaagg ttcaagtgtc gtttttgaaa gatgaaatcc aacaattaca
    28441 aaagaaagaa gtacaaagtc ggtcagaatt aacgtacaca cctgaaattg aatcctttat
    28501 gaatcgtttt gttgctttgt atatgaatac attcgataat catgaacaag aacaacaacg
    28561 tcaagaaaca ttaaaaaaag agttttatgc acaaaacttt attcaagaac caggaagtgt
    28621 ttcaggtgtt cgaaaattaa ctgctgcttc gtttattgga ttaaaagaaa ttgaaaccat
    28681 tccaactgct agttataaag taacttatga tattcgctcg atgaatccag agagtaagaa
    28741 agaaacaagt gtgacaatca cacaagtact caatcttccc ttttctattt caaacaaggg
    28801 ctgtcgtatt attgcttatc cttattttac ggcactacca aagaatacag aaaaacaacg
    28861 aatgattact tatgatgaca cattatacga aacggttgat gcgtcggaag cgaaagatgt
    28921 tgaaggattt gtggaagatt tcttagataa gtatgcgaaa aacaatgcaa aagatatgtc
    28981 atacatgatg aaagaacctg aaggactcaa tcaaacagct aaagttgatc aagtagagat
    29041 gaaagtattg cgttataaag aaacgaaaaa accttttatt gtaaaagcaa cggtcgtctt
    29101 tcgagatggt gatacgtcac ttttacaaaa agaacaaatg acgattgtca ttacgaaaaa
    29161 aacaaatcag tattatgttg aaaaattaac acataattgg ttggatagga aaggagaaga
    29221 acggaaatga cactgaaggg aattgtagat tttatattaa cgcaaggtgg ctatttttta
    29281 ataatggtgg ttgtttatct gggattgcga aattttatga aatcaaaagt aggaaatgca
    29341 ttaatatcta tcgcagcagg tgccgcagtt tatttcttct taaatgatcc aacacgtgta
    29401 tttgatgcgt ttggtaaaat cattgaaaaa ttatttatgg attaggagga tagtgaagtg
    29461 gattgggaac cttatgatta ttcgattgtt tatgcggaac cttatcgcgt acaacagatt
    29521 acaagcaatt ggcgtcttcc atttccagtt gcattagtca atgcagcaac ttttttgttg
    29581 gtcgtaatct ttgaatattt atttttacgt tcatttattg gttgggtcat ttcttttttt
    29641 ccaccagcag gagttgtttt ttatatagga ctcccctatt tatgtgtgtt agcactggca
    29701 aaactaaaac ctgatggaaa aaaagtgcat ttttatttat gggattggtt ttgttatatg
    29761 attcgtatga tgaatggtga ttgtgtgtat tataactggg acaaggtatc aaacaatgca
    29821 ttggaagaat tactttttgt gaataagtat gtttatttag aggattagaa aaagggacaa
    29881 tcaactggtt gttccttttt tattgggagg tgaaattcat tggtgcttgt aaaagaacca
    29941 gtaaaagcat tcaagaaaaa ttgtttgtta aatggagagg attgtatctg ggcctactat
    30001 catgtttttc cacgaacaat ttcaaaaaac acaccaaaaa agacacagga aataaaagaa
    30061 atgatggctc gtttactgtt gcttttagca gattatgaag atgtggagtt ggtgatgtat
    30121 ccacgaacaa tgaatttacg gacgaagttt gacacattag gaaagggatt ttctcccgat
    30181 agtcaagcaa ttggtgcgta ttatgcaaaa gaaacagtga cattgttgga acgagaaggt
    30241 caagtctatc gtccttcatt tattttagga atcaaagtca aagaaaaagc aacggtggag
    30301 agttttcatg atgtaataaa tctaatctgg ttagatttag caggaagttt agcacgctta
    30361 tttggattag aagtagatga tttggaaact ttgttttctg aaagtgatcc ttatgtaaaa
    30421 gaagtgacac aattacttta tagcttttct attcgtccct tagtaaaaaa agagctgttg
    30481 tatctcaatc gtctgcaata tattcgagga atggctcaca aagtagaaga acaaaataat
    30541 tgggaagtac tgaaagacaa tcaaggaacg tatgtacgta taccgaagga acataaaggc
    30601 cttcttcaat tagaagatga gttagggagt agttatttag cattgttgcc tattggggaa
    30661 acacctgaaa atatgagaaa ttgtgatcta tttcaatttg ctcaggatct ttctttccca
    30721 gcagaattgc gaatgaaaat tcactatcca cgcttgaaag gacagggagg tattggttta
    30781 aaactgaatt ggctatcgga acggttcaaa acagagcata aggaattggt tcaaaatgat
    30841 gaagaaccta gtgaacgctt attaactgtt cgaggattag taaaacattt aaagcaacga
    30901 gtagataatg ggcaacctgt cgtcgattgg atcgcatgtt ttattgtata cggtcaaacg
    30961 gttgaagaag ttcgtcaaaa tagtgcaacg gttattcaac aacttcatac gattatccat
    31021 atccaccgtg caaaaaatga tcaacgagaa ttgttctata aatgtttatt agggcaacca
    31081 ttgggacggc agttacattg gaagcaccga acaactgtag aagcagttgg cgaatggttg
    31141 ttttcagcag tatcagaatt aggtatggaa tccggctggt atattggtcg gcaagataca
    31201 ttaggggatg gtggttctcg aggagaaaaa acaccattag aagaattaat ttatgcgtca
    31261 aatcgttttg tctttttgaa tcttctagcc attgcggaag gaatcaaagg tgcgttgttt
    31321 gatgcgcctc atgtagcagt aactggaaaa accggtaaag ggaagacctt tttagttggc
    31381 ttgctctttt tgtatagttc gtttatgaat gtacaaagtt tatttgtgga tcccaaagga
    31441 gaaaagcgca aatggtttac gaaactcatt caagacccgt attatcagaa acattaccct
    31501 ttgtttgtag aacatttgca aaaaatgagt tatgtcacgt tagatgctac aaatccccgt
    31561 aattatggtg tattagatcc aattgtttgt ttatcccgag tagatgcgaa acaagtagcc
    31621 caggatatga ttgatgagtt aagtccgtta ggtaccaatc accttttaaa aggggcggta
    31681 ttaaagggaa tccaagaagt tttagttcaa aaagaagccg gacaacaagt tggcttgatg
    31741 catgtggtcg actatttaga acaaatggaa caaaaagagg ttcgagaata tggtgagttt
    31801 ttacgcttga cggtagaaga ttcgatttta cgtttaggat tttcttatgg agaaaacccg
    31861 ggattagact ttaatgccaa aacaacgatt ttagagatcc aagatttgaa attaccaaat
    31921 gacaatgtcc gaccagaact atatacagat gcggatcgaa agtctttgtg tttgatgatt
    31981 agtttaggtc gtttttgtga aatgtttgga aaacgagata gtagtaagaa aacagcgatt
    32041 tattttacag aagcatgggt atttaataat tcgaacgcag gacgtagtat tattgcagca
    32101 atggcacgtg tggggcgttc ccagatgaat caattagtgt tagatacaca atttattggt
    32161 gacttaggtt cagataaaga aaaagggaat tttggtgtcg tctttgcttt tgatgaagac
    32221 agtgagcgag aaaagatttt gaatcattta cgtttggaag taaatgaagc gaacttagct
    32281 gaaatgagcc acatgattaa aggacagtgt tggatgttag atccctatgg acgtgtagga
    32341 aaactcaatg tacacagttt gtttgaagaa atgaccgctg cattacaaac aacggaaaaa
    32401 acaagtcact ctcaagtaga agaacaggat acgttaatca tgcaatagaa atgaggtgaa
    32461 acaatggctt atataaagca taaggaaaag aaatggtgcg ttttgttttt tttaacgatt
    32521 ggattcggac tgctttttgt tggtggattg gtgtatgcgg cagaaaagca ggtggatcaa
    32581 tcgatctatg agattgggaa atttcatatt gatacctttt cttcttattt atctgatagt
    32641 gaattttcat taggcaaaat gttatttggg gcaaatggca cagattttct aaaaatgatg
    32701 acgaatatgt tctttagttt aagtaaattg atttggcaag gccttgattt tgtcattgag
    32761 aagctctatg aaggagctgc aatgaacgag ttaattcacc agtttttcac tttttcggat
    32821 actatttatt caaaaatata cgatcctatt ggtgcgggga ttgtcgtctt gtatgtgtta
    32881 tatctattta ctcttcgtat atttagaggg aaacattacg caaaaatggt gtttattcga
    32941 tttctcttcg ttgtcatttt ttcaatggtt tggtttggct atggtagtaa cgtacctagt
    33001 aaaggagaag cttttaccaa agggttaaat gatttgtccg tggaggtaga aggctttatt
    33061 ttttctgcta caaatagtct agatgaattg gcgacaaaag atgtcaatgg tgttttagtg
    33121 ccaacgacac aaaaggaagc cattcaaaaa gtgcgccaac tttattataa agcggctgtt
    33181 gttgatccgt atatattgtt aaattatggg acaacaaatt tagaagaatt gtatcaagca
    33241 gagattgaac cacaagaatt tctagccaag gaagcgtcaa gtgaagaatt atctgatatt
    33301 gagacaaaag tgaccactgc tgcaggagaa gatagaggga aaaagaaggc ttatcgtgtc
    33361 tatattcaac caactaaagc tgtttataaa gcagtcgttg gttctatggt gccattgctt
    33421 aatctaagta ttggattacc acttgtagtg attggcatga ttcgttttat ctttcaaatg
    33481 ttggtgttgt gtttattact tgtgattcct ttttcattac tcgcttcttt tgttccagta
    33541 tttgattata tgctcatgaa taccttaaag tcctttttgg gcttgatgtt tcaaaaatct
    33601 ttttacagtg tgattatttt ggtggcattt ttgatctata acatagtaga cgatttaatt
    33661 ccaatgaata ctgtttttgg ttttttagga aatatgtttg tcaaagcact cctatcaatt
    33721 ttactcattg taaaacggaa agatttatta aagaaactag ggttaggagc tgcagatcaa
    33781 actataaaca acacgaaaaa catagcaagt cattctttgt ctcaaatgaa acaactgcct
    33841 ggtcaactat caactgctgg acaaaatttg gcaatcaaag gtgcaggatt agcagggaat
    33901 gtgtatcgag caacgaatcc actcgcttct gcagcgatac atggatttca aaagtttcaa
    33961 caaaaacgta caccacaaga atcggtaaaa gggaggcaag tggttaatcc ctttgaacta
    34021 ggtgggcgtt caaatcaagc cttgccttat agtggtcgtc atgttcgtta tcatggggta
    34081 gagcctgaaa tgtcttctac caaatcgaaa acagagtgga attctggtct ttcaaatggt
    34141 ttgattccaa attcgtccat gaattcagta agaccaaaac aacaggtaca atcattggca
    34201 caattgagta agcaacaagg gaataatcaa agaaaaccat tgttaaataa aggtatcgct
    34261 tttgttggtc aatcgattcc gttgccttct ggaaaagtac ccgtgccaca attaacagct
    34321 gctggttctc tttcacaaca gattcgccaa gtgaaaaatc aggcgaaaaa tgggagttat
    34381 ccagcaatgc agcagttatt gaaccaatac tctgttccta attcgtcggt agccactcta
    34441 tcaggaaatc gttctgtgca acaaccgggg caacgttcgt ctgaaagtaa accgtcgatt
    34501 tccgcaccaa aatcgttaca acctgcattt tcttctttag ataagccaac agagcaatcg
    34561 gaaaaaatag aaaaggatcc taaaacactg ccgattttgg acgcaaaacc aggaactgat
    34621 tttactttac caaaaggacg aacagtccaa aaaccattat cgaagaaagg atgaggaaga
    34681 aatgaagaaa gggtgtctag gttgtcttct tttctttatt cttccctttt ttctattgcc
    34741 attctttttt tcaggagaac tttctgagca tggaggagaa gctacaggtc tagctgtcgc
    34801 ttttaatggg gaatataccg aaggattacc gattttttca gagattaaag gacgtggtcc
    34861 aatcacagat gaacatgcgc aagcagcagt tggtgcagct gtaaagtatc agttattacc
    34921 gagtgtgatt ctttctcaat taggctatga atcagcttat ggacagtcac aatctggaag
    34981 aaacgatacg aacttttttg ggattacctg gtttcctggt tgtccctacc cacaaggttc
    35041 tgcacgtgga attggtggtt ctgaaggtgg tttctatatg aagtttccga acttaaaagc
    35101 ttgttatgac tattatggct ttatggtggc gagccaaagc aactttaatc aatgtgtggg
    35161 caataaagat cctggaagtt gtttattgat tctaggtcgt ggaggctatg cggcagcagg
    35221 tattaccgaa gggagtccgt attatcgagg agcaatgagt atcataacgt cctataactt
    35281 aacggaatat gatacttttg cgatcaagca ttggaatagc ctaccaacaa aaaaaacttc
    35341 gcttattgca ggaaaaagta aaggcaatgc gaaggttttg gatcggatca ttggacgatc
    35401 cgtaaataat ggggaatgtt atggtggtac ggcatggtat gtatctcaat taggtggccc
    35461 acagttaatg ggttctggac acatgtttgc gatgttaata ggggaagatt ataactggac
    35521 aacctatggt tggcaagtaa tcatgaaccc aaaaccaagt gatttacaag caggagatgt
    35581 ggtgaattgg caagcaggtg gtgcattgtc tcctggaatc tatggtcaca ccggaattat
    35641 aacagatgtt tcatatggcg gacaacattt ctctagtgtg gaacagaatg ccgagcaagg
    35701 acggattatg gctcggtatc aacgcacgta caatcaaaca aaaattcgta gtctggttcg
    35761 taaggtgaaa taagaaaggc agtgactaga aacgtgaaaa aagggataat tttagtattt
    35821 agttggttat gtttgatagg tcttactggt tgtcattggt tttcttctga aaaacaaaaa
    35881 acacaattga gaacgacaga agaaacaatc gaagcagtaa aagaaacaag tggaactctt
    35941 caaagtactg aaagtaacag tcaaattggt tcattagaag acgagacagc gcaacaaatg
    36001 caaatagatg aaatgacaaa aaatttctta aaagattttg ctagaaaatg gagtaatttc
    36061 acagatgttt ataaacgcaa tcaagctgtg agggactata tgacagatcg ttgtattaaa
    36121 gacaatagta ttgatgtaga cccgcatgta caaatgaaaa caactgggaa actttaccag
    36181 gtgacacaag atatagagaa tccggatcaa tatttgttat ttggagaaga aacaatgaat
    36241 aaggttactc aatttgtatt acttcaattg aaagtaggtc aagaagctca aaagattgat
    36301 gcgatcaaga tttattatgt acggagtgcg tattaaaata aaaaatgaac actcatattt
    36361 attatacacc gaagaaatag aaaaaaacag actgtttttt taagaaaaac tatgtcattc
    36421 tttacttgaa aaaagttgtt ataggacaga aaggataaaa caaaaacgga aaaaatatgt
    36481 cttatgaata actataagaa ctattctatt ttttaggaga tggatacgag atgaaaaaac
    36541 taattggcta tagtgtgatg tgtattggat ttgttggtgt gggattgttt gtatcgactg
    36601 aatctgcttc tgcggctgaa tggtataatc ctatctggta taatccgaac attgaatgtc
    36661 aaaaaggcta tccgtatgga ccgacaaatc tgaaaaatgg aaaatgtaga gtaaattgga
    36721 atggcgttgt agatagtgta attactggtt caatggactc tgctctacat ggctttacgg
    36781 gtcactagaa ggaggaagaa aatgtctgaa gagaaaaaag aaatcgttga atcattagta
    36841 gctttaaaag attctctttc taagattgaa tatgttgata ggcaagaaat tcaaaaacta
    36901 attgatgata ctattattga aatccaagat gcgcgttgtg aaggtataaa aatttctgta
    36961 gctttatcta aagtaataga aaaaatgaac cgttcattag cgtttaatgg attgaagcta
    37021 gatagacaaa ctagtctgat atgggatcat ttaaaagact tgtatgacaa gtctaaaaga
    37081 agtgaacgga ctgctgtatc aatcttaaaa ggcctttggg gaatgaattc ataaacaaga
    37141 tatttaaaaa gtaacttatt ttttatggag gagaaattat gaaaagaata attaaaatat
    37201 ttggtgtttt aggtgttttc ttattaggtt taagtacttt tgttagtact ggaacaagtg
    37261 tgtatgccac agaaaaagtg aatacagttg atttagaaat aaagaataaa ttagtagatg
    37321 aattaacttt ttattttgat acagttggtt ttttagacga acatggtgta taccatataa
    37381 aggattttgg tgctattcgt gaacgaatca gtcaagataa cgaacaagga agagcagcaa
    37441 gagtctttta tgaacatatt attgttccta gaatggagaa agttgattgg aaacgatatg
    37501 ctggatgtgt agccattaat tctcttcctg gaggaggagt ggcttgggct ataggtacgc
    37561 agggagattt tattgaagca ttaaaagctt ttaattttga aaaagctgcg gatttattat
    37621 taaaaattgc agaaaaacaa ttaggaaaag ctgcagctaa agagttagct aaggtaaata
    37681 ttgtagcagg aattgcagca gctgctgcta gctgtgcatt agagtaaaag tttatgaaaa
    37741 gtttagaaca agagcatatg gcttttaaac aagcaatgtt taaagaaaat atctatttaa
    37801 accataacta tattcgtgtt tctaaggcat gttcaccagt attgaatatg cttggaggtg
    37861 gaaatggatt atatcattta ctgtttgtcg atgtttgttg gttaattttt ttaccagatg
    37921 aactagttat agtcaatgaa aaaataacaa gtaaaaatga agtgtttaat tattctttaa
    37981 caagaattaa ttacaaagag attaccaagt tttcactaga aaaagttcct ttttggggag
    38041 aattttgttt aaaaattaaa tgtaactgga aaagaatgta tttctatatt gatggtgatg
    38101 atgctcttac attcggtaaa actacttttt ctagttttaa ttttcagttt ctattaaaaa
    38161 ataattttta tggtttattg aaataaggtt gcgaaggaag atgtagagtt atttgcactg
    38221 ttgtgttggt atatctctta atgctgtgtc tttttgggga gattaattag tggagtgatt
    38281 atatgagtat agatgattgt ttagaagaac aaataagatt atttttaaag tttttaaaag
    38341 ttaaagttca aaataactac ttcacatgtt atagttatgc acaccctgtt ttaaatagtc
    38401 ttccttttaa ttctttagtg caaggattga ttacaaatat tgttttatta gtctgtacac
    38461 caaatgaaat atttgtaaaa aagataggca gtggtatttc ttcagtaaga ttaaaagaga
    38521 aagaatatac tcaaaagatt attcatattc ctaagcctga aattaagcgt tttgagataa
    38581 aagactattc tgctttaggg ctgttctcaa ttggttactg gtgataaaaa ataagaatac
    38641 taaaactata cgtagaatat aagtaatcgg catcaatcct aattatgtga ttgaatgccg
    38701 atttttgtta cgcgctattt ttaattatct gtaagaagaa taattttatt ttactcatat
    38761 tcgttttcac tttgaagaag taaattgcaa tttcttataa gttaagcagc tatgcaaaag
    38821 gctatttatt ctattgtgaa atataagatg tagttgaaac aggatatata gaatcaaaat
    38881 acgaatctta ttttgatttt tctagttttt tgaaaaaagc accctttgat cgttgtatta
    38941 gtggaaaaga gaaaaaggag catacttatc atgtcacaaa actacaacta aatatctaaa
    39001 caacaccaat ttgatgccta ttgaaaaaaa gtattgcgaa atgaagccaa gtctattaga
    39061 aaatgaaata atagaataaa agaaaatggg aaaccgctga atgatgtaaa tgaaggtaag
    39121 tatccacgaa ctaattttga tgaacaagat atctattttt tatttgtatt ggagatacta
    39181 gtttaatatc aaaagttatc agcagtcata attagttatc caatgtaaaa agtacaaaca
    39241 atagttgtta tgcagaatag tttatgaaat atattacata ataagaaa
//
LOCUS       DAZAFF010000015        36803 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000015 DAZAFF010000000
VERSION     DAZAFF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.015"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..570
                     /locus_tag="ACG9BB_000787"
     CDS             115..570
                     /locus_tag="ACG9BB_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836754.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            932..1207
                     /locus_tag="ACG9BB_000788"
     CDS             932..1207
                     /locus_tag="ACG9BB_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836755.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            1220..3298
                     /locus_tag="ACG9BB_000789"
     CDS             1220..3298
                     /locus_tag="ACG9BB_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN2836756.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            3461..5344
                     /locus_tag="ACG9BB_000790"
     CDS             3461..5344
                     /locus_tag="ACG9BB_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN2836757.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            5356..6303
                     /locus_tag="ACG9BB_000791"
     CDS             5356..6303
                     /locus_tag="ACG9BB_000791"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFN2836758.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKRPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            6323..6850
                     /locus_tag="ACG9BB_000792"
     CDS             6323..6850
                     /locus_tag="ACG9BB_000792"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFN2836759.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6913..7566)
                     /locus_tag="ACG9BB_000793"
     CDS             complement(6913..7566)
                     /locus_tag="ACG9BB_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFN2836760.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7698..8540
                     /locus_tag="ACG9BB_000794"
     CDS             7698..8540
                     /locus_tag="ACG9BB_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN2836761.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8698..9594
                     /locus_tag="ACG9BB_000795"
     CDS             8698..9594
                     /locus_tag="ACG9BB_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN2836762.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            9597..10310
                     /locus_tag="ACG9BB_000796"
     CDS             9597..10310
                     /locus_tag="ACG9BB_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFN2836763.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            10330..10848
                     /gene="msrA"
                     /locus_tag="ACG9BB_000797"
     CDS             10330..10848
                     /gene="msrA"
                     /locus_tag="ACG9BB_000797"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN2836764.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10845..11072
                     /locus_tag="ACG9BB_000798"
     CDS             10845..11072
                     /locus_tag="ACG9BB_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFN2836765.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            11224..11775
                     /gene="lepB"
                     /locus_tag="ACG9BB_000799"
     CDS             11224..11775
                     /gene="lepB"
                     /locus_tag="ACG9BB_000799"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN2836766.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11858..12400)
                     /locus_tag="ACG9BB_000800"
     CDS             complement(11858..12400)
                     /locus_tag="ACG9BB_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFN2836767.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(12637..13035)
                     /locus_tag="ACG9BB_000801"
     CDS             complement(12637..13035)
                     /locus_tag="ACG9BB_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN2836768.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            13295..14155
                     /gene="ylqF"
                     /locus_tag="ACG9BB_000802"
     CDS             13295..14155
                     /gene="ylqF"
                     /locus_tag="ACG9BB_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFN2836769.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            14148..14915
                     /locus_tag="ACG9BB_000803"
     CDS             14148..14915
                     /locus_tag="ACG9BB_000803"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFN2836770.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14971..15828
                     /gene="dprA"
                     /locus_tag="ACG9BB_000804"
     CDS             14971..15828
                     /gene="dprA"
                     /locus_tag="ACG9BB_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFN2836771.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKIKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15950..18028
                     /gene="topA"
                     /locus_tag="ACG9BB_000805"
     CDS             15950..18028
                     /gene="topA"
                     /locus_tag="ACG9BB_000805"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFN2836772.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17997..19373
                     /gene="trmFO"
                     /locus_tag="ACG9BB_000806"
     CDS             17997..19373
                     /gene="trmFO"
                     /locus_tag="ACG9BB_000806"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFN2836773.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            19571..20476
                     /gene="xerC"
                     /locus_tag="ACG9BB_000807"
     CDS             19571..20476
                     /gene="xerC"
                     /locus_tag="ACG9BB_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFN2836774.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            20513..21061
                     /gene="hslV"
                     /locus_tag="ACG9BB_000808"
     CDS             20513..21061
                     /gene="hslV"
                     /locus_tag="ACG9BB_000808"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFN2836775.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            21075..22475
                     /gene="hslU"
                     /locus_tag="ACG9BB_000809"
     CDS             21075..22475
                     /gene="hslU"
                     /locus_tag="ACG9BB_000809"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFN2836776.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            22493..23284
                     /gene="codY"
                     /locus_tag="ACG9BB_000810"
     CDS             22493..23284
                     /gene="codY"
                     /locus_tag="ACG9BB_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFN2836777.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            23366..24241
                     /locus_tag="ACG9BB_000811"
     CDS             23366..24241
                     /locus_tag="ACG9BB_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFN2836778.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(24373..25011)
                     /gene="plsY"
                     /locus_tag="ACG9BB_000812"
     CDS             complement(24373..25011)
                     /gene="plsY"
                     /locus_tag="ACG9BB_000812"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFN2836779.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            25138..25572
                     /locus_tag="ACG9BB_000813"
     CDS             25138..25572
                     /locus_tag="ACG9BB_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFN2836780.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25738..27783
                     /gene="parE"
                     /locus_tag="ACG9BB_000814"
     CDS             25738..27783
                     /gene="parE"
                     /locus_tag="ACG9BB_000814"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFN2836781.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            27795..30245
                     /gene="parC"
                     /locus_tag="ACG9BB_000815"
     CDS             27795..30245
                     /gene="parC"
                     /locus_tag="ACG9BB_000815"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFN2836782.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            30522..32753
                     /gene="pflB"
                     /locus_tag="ACG9BB_000816"
     CDS             30522..32753
                     /gene="pflB"
                     /locus_tag="ACG9BB_000816"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFN2836783.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            32997..33758
                     /gene="pflA"
                     /locus_tag="ACG9BB_000817"
     CDS             32997..33758
                     /gene="pflA"
                     /locus_tag="ACG9BB_000817"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFN2836784.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            33843..34769
                     /locus_tag="ACG9BB_000818"
     CDS             33843..34769
                     /locus_tag="ACG9BB_000818"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFN2836785.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            34951..35844
                     /locus_tag="ACG9BB_000819"
     CDS             34951..35844
                     /locus_tag="ACG9BB_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFN2836786.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            35978..36103
                     /locus_tag="ACG9BB_000820"
     CDS             35978..36103
                     /locus_tag="ACG9BB_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFN2836787.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(36237..>36803)
                     /locus_tag="ACG9BB_000821"
     CDS             complement(36237..>36803)
                     /locus_tag="ACG9BB_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836788.1"
                     /translation="LFFLCYSYLKTSHAPSSALGWLLLDHSDITEAEENPFFLILSSL
                     CGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFLAILFYSFNHNTLFFFIMNGLV
                     VILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFSFFVLALMPLTNPSTIQVAGN
                     LLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT        12910 a         6308 c         7745 g         9840 t
ORIGIN      
        1 taaagtgggc agatacgtca ttagaagaaa aaacataaga tgtgaccact tacataaatt
       61 tgtgctaaac tgaggtagta caagaataaa ttacatcata aaggagtctt tttaatgagt
      121 aaagaaatga cagcgctaaa attttatttt agaaatggag aaacctggac gatcaaccgc
      181 cgtcatatcg gcgatctttg gatcaaacaa atcacaacaa gttttggacg aatcaatggc
      241 agtgaatttg ttgaaatcca tccgtgtgct ggatttaaaa tcgaaatctt ccatgaaggg
      301 gacgcagttg cgacacatga tatcaaccta ggcggactgg aaatgggcat gtttaatcgc
      361 gcattgaaat acgaagatat cgagcgaatg gaaatccttt accgaaatgg cacacctgat
      421 cttgtgtact tcccatatct agataaagga acagaaggat tagacaatca atatcaatca
      481 acaaaaatca gcgaaaaaac aggtaacttg tatatcgtga tcaatccaga tcaacgtgta
      541 gaagatgtat acggggaatt tttcgaataa atcgattttt acgcaaaaat gaggctgtga
      601 aagagatgtg tcttattact actgataggc tgtctcttcc acaccgttta ttcagaataa
      661 gggactgtga caagattttt gtcgcagccc catttttttg ttgtgataaa agaacaagaa
      721 aggcaggaat gggaaagtaa acatcctttt gtcagaaact tttatctttc ttaagtttta
      781 cttgatatct gatactattt ggaatagaat gaggcgttga aaaatttatg gaaatttcta
      841 actagcaata aacgatgaaa caatgaaata tttttcatcc aaaaagaact ttgctataat
      901 gatggaagtt tgtgtatagg agatctggaa aatgagagag agttttgaac aacaaaaaaa
      961 actgttgcat gatcgctatg gcgccctttc gatggatgac cgccgtcaaa tactttgtaa
     1021 gttgcgtaaa agaaatatat tgatgtatcg tcagttggaa cgtctgaaac atgatctgct
     1081 tcgtttggag tcaaaacgtg tacagtgtga attagagggg aaccaaacac aggttgaagt
     1141 agtcgaaacc aaaattttaa agaaaaaaga acaattttta aaaatgctta cacaaaataa
     1201 aaaataaagg ggatggacga tggaatttgc tgagttagtt attgtatttg cagtaacgat
     1261 caccttatcg aacatagcta gccgcatttt gccaatgatt ccagctccac tgatccaaat
     1321 tttccttgga gtggtattag gtctgacaga gtggggacaa tcaatcgatt ttgaacctga
     1381 attgttctta gttatgatta ttgcaccatt gttatttaga gaaggagaaa aagcagatat
     1441 ttcttcgatt ctcaaaaatt tcgggacaat tttgttcttg gcatttggag gagtcatttt
     1501 gacccttgtc ggggtaggtg cgacgttatc attcctgttg ccaagtgtcc cacttgctgc
     1561 ctgctttgct tttggagcag cgttaggtcc gacagatgcg gtagcagtca gttctctttc
     1621 tggacgagtg aatatcccta aaaaggcgat gcatattttg gaaggagaag gtcttttgaa
     1681 tgatgcatct ggtgttactg ccttccagtt tgctcttggt gcgctgatca ccggatcttt
     1741 ttcagcagta aacgcgggga tgagtctagt agtttctagt attgggggag ctttgatcgg
     1801 ctttttgctt gtctggttca aacaaaaaat aattcatttg atcgaaaaag catcagcaca
     1861 agatgtcaca gggtatctgc tgatcgaact tttgcttcct tttctagcct atgtattggc
     1921 agaggcattt gatgtatctg gaatcattgc tgcagtggca gcaggtattc tccaagcttc
     1981 tggttttagg aaaatctctg tgtttgatgc agaattgtcg agcttgtccc acagtacatg
     2041 gacaactatt gcttttacac tgaatgcatt agtatttata tttttaggaa tcgaattgac
     2101 gcaagtgttc tcgccagtat ggggagacgg tttatatcca aacggtcttt tattagcgat
     2161 tatcgtatta atctctgtca tgctttttgt gatcagattc atttctatca gtctttttta
     2221 tgtattcaaa gatggcagta aaaagttcaa aaaacagttg aacgagatct tgatcttaac
     2281 atttggcggt gtaaagggaa cagtcagttt ggctacgatt tttattttgc cattttcaat
     2341 caataatatg atgttttacc agcggtcttt gcttttgttt ctgacggcgg gtgtcatctt
     2401 agtgactttg gtcataggga tcattgtctt gcctatgctg acggaaacag aggaagcgaa
     2461 aagcacggat ttgaatgctt tgatgatttt agaagaggtc gttgaagttt tgcgaaaaga
     2521 aataaaggaa attgatcgta atacaaaaga atttttagcc actgaagcag tgatagaaaa
     2581 ttatcaagaa cggatacgtg atctgtactt ggaagattta accgatgatg aaaagcaaga
     2641 agtacaagaa atacaagcac tgattttgtc gatcgaacgg gatggattgg atgaaagtta
     2701 tcgttcgggc aagctttctt ctaatggtta tcgtttttac tcccgttttc tttctcgatt
     2761 cgaacattct atcacaagcc agattttgtc ttttatcggc ttttggttca ttgttgtccg
     2821 taggttaatg cgaatcgttc tccatccgaa aatgttttgg caaagaagat atgctgatcg
     2881 tcagacattt attagtgatg aagatatcca agaaatcaga gagacatatc aaaaaaatac
     2941 tcagttgatt attgaaagtt tagataactt gactgatatt tatgatgaaa cgatggttca
     3001 attttttatc cagcaacgca aacttgaagg aataaaaatg aactcgggga atttgatttc
     3061 ttcgtggatg atccagcaag atagtctgtt tacgaaaaaa atgctaagag gttattatct
     3121 tgaaagaaaa atcattgatg aatacgaagt tgctgaaaaa atcacgacgt tttctgcgaa
     3181 cagctatcgc aggaatatca atttgttaga atcttatacg atgaacaaac catctgataa
     3241 cttttctttc cgatttgcgt ttcgagcaaa gtcaaaaaaa acgagtccaa taaaataaaa
     3301 aggtaaaaat tttcaagttt tataaaaaac aaaacatatc agaacaggat cgaatcggtc
     3361 ctgttctttt tttgagaaca caaaaagaac aaataccaaa aaaattttcc tgaagttcac
     3421 tatttttatg atacaatatc caagataagg agggaacatg ttgaaagaat taaaagtaac
     3481 tgacctcaaa aaaacttatg gtgaaaaaga tttgtttgac cagatttctt ttttggtcca
     3541 cgaaaaagac cgtatcgggt tgattgggac gaacgggacg ggtaaaacaa gtctcttaaa
     3601 tattattgcc ggtatcgata gcagcgacgg tgatcggcaa acagtgtttt atccaactga
     3661 ttaccgtatt ggttatcttt ctcaagcagc tgaattttcc gatgaattaa ctgttctcca
     3721 agcagtcttt caaggaaaca gccccttgat ccaaactgtt cgagcatatg aggaagcatt
     3781 gatcgaacta ggggaaaacg gagaagaccc tgatgtgcaa aaacgttatg ctaaagcaga
     3841 agaacaaatg aataaagaag atgcttggac tacagatacc aacgcaaaga ttattttgca
     3901 aaaattagga atcagtgaat tagataaaaa aatcagtact ttatcaggtg gtcagaaaaa
     3961 acgagttagt ctggcgcaag tgttgattga tgaacccgat ttactattac tagacgaacc
     4021 aacaaaccat ttagattatg aagcgatcga gtggttggaa aactatttga aacaataccg
     4081 tggcgcacta ttgatggtta cccacgatcg ttattttcta gatagagtag caaaccgaat
     4141 cttcgaactt tcatttggaa aattatacga gtataaagga aattatgaag cttacgtctt
     4201 agaaaaagca gaacgtgatc gtgtagaagt agaacaagaa gaaaagcgca agcggcttta
     4261 taaacaggaa ttagcttgga tgagggctgg cgtacaggca cgtggcacca agcagcaagc
     4321 acggatcaac cgtttcgaag atctaaagga gaacttgtac caagtaaacc aagaagatga
     4381 tttagagctg aacctagcga cacaacgttt agggaaaaaa gtactggaaa tcaaagatgg
     4441 cagttaccgt ataaatgacc aaacactgct agaacattta gatttattga ttcaatctcg
     4501 ggaaagacta ggtattactg gtaaaaatgg tgcgggaaaa tcgacattgc ttaatattct
     4561 agctggcaga attcccctgg atagcggtac aatgtctatc ggtgaaacgg ttcatcttgc
     4621 gtattataca caagaaaatg aagaaatggc accagataaa cggatgattg cttacttaca
     4681 agaagctgct gaagaagcaa aaacagcaga tggcagtcaa atcggagtag cagaattact
     4741 tgaacgattt ttatttccaa gatttatgca tgggacactg ataggcaaac tttcaggcgg
     4801 agaaaaacgc cggttatttt tgttgaaact gctgattcag caacctaatg ttttattatt
     4861 ggatgaaccg accaatgatt tggacatagc aacattgacg attttggaag attattttag
     4921 aagttttcca ggtgctgtga tcactgtttc ccatgaccgt tatttcttgg acaaggtagc
     4981 agataagtta ttagtattcc aaggaaacgg aaaacaagaa ctttattacg gaaacatgtc
     5041 cagttattta ctgaagcaaa aagagaccca gcagcctgct gaaaaagcga aaccaaaaat
     5101 acagagcaag gaacctgctg aaaagaagaa attatcttac atggaacaaa aagaatggga
     5161 aacgattgaa gatgagattg cagaacttga agaaaaaatc tcattgctcc aagaagagat
     5221 gaatcatcaa ggagataatt tcactcgatt acaagaattg caaaacgacg tatctgaaac
     5281 agaagcacaa cttgaagaaa aaatggcacg gtgggaatac ttgagtgaat gggtagaaga
     5341 ttaggaggaa aaaggatgga acaagcgtat cttgacttag gaagaaaatt gttggaagaa
     5401 gggcatcgta aagaagacag aacaggcaca ggcacattaa gcttattcgg ataccaaatg
     5461 cggtttgatc tgcaaaaagg gtttcctttg ttaacaacga aacgagtacc ttttggattg
     5521 atcaaaagcg aattgctttg gtttttaaaa ggagatacga atatccgcta tctattacaa
     5581 aataaaaacc atatttggga tgaatgggca tttgaacgct acatcaaaag gccagactat
     5641 aatggaccag atatgactga ttttggccat agatgtttga cagatcctga attcaatgaa
     5701 gaatataaaa agcagagcaa gcttttttgc gagaagatcc taaccgatga ttcctttgct
     5761 gaaacatacg gcgatcttgg acacatctac ggctatcaat ggcgtcactg ggaaacaaaa
     5821 gacggtggct ttatcgatca aatcaaacaa gtgatcgaag caatcaaaaa gactcctgat
     5881 tctagaagat tgatcgtttc tgcatggaat ccggaagatg ttcctagcat ggctttgcct
     5941 ccttgtcata cgatgtttca attttatgtt caagagggac ggctaagctg ccaattgtat
     6001 caacgcagtg gggatgtgtt tctaggcgtt ccgttcaaca ttgcaagtta tgcgttgctg
     6061 actcacctaa ttgcacatga aacaggttta gcggttggcg aatttgttca cacattagga
     6121 gatgctcatc tttatatcaa tcatatcgaa caaatgaaag aacaattgag ccgagaaata
     6181 cgcgcatttc cgactttagt tttgaatcag gaaaagcagt cagtatttga ttttgagatg
     6241 gaagatatcc aattagaagg atatgaccca catccggcga tcaaagctcc tatcgctgtt
     6301 tagaatcaga aaggatgaga aaatgttagc tgcaatttgg gcacaggatg aaaacggatt
     6361 gattggaaaa gaagaccgtc ttccttggcg tttgccaaat gatctgaaat tcttcaaaca
     6421 aatgacagaa gcaaatacac tagtgatggg acgaaaaaca ttcgaaggga tgggtagccg
     6481 tccgttgccg aatcgtcaga cgattgtttt gacgagagat tcttcctata aagcagaagg
     6541 cgtgcatgtc atgcatagtg tggaagacgt gcttgctctg gaaaaagaaa cagacggtat
     6601 ctttttcatc gctggagggt cagctgttta tcaagaattt ctgccatttt gtacgatatt
     6661 ataccgtaca gtcatccatc atgtattcga tggagatgct tattttccac ctgttgactg
     6721 gtctgattgg tcactgatca atactagtca aggagaaatg gatgaaaaaa atcgttatcc
     6781 gcatcaattt gaaacgtatc agcgtaatga gaatcctgta aaaagcggga caggcgaatt
     6841 gacgatataa aaaagctatg gatagactag ctgttattta gagctgtccc atccatagct
     6901 ttattttttt tattaagtaa agaataaaac agagaagtac ataaaacctg cacctaacat
     6961 aacaaacaaa tgccaaacaa catgcatata gcggatagaa ttcaagctat aaaagaatgc
     7021 gcctatcgtg taagaaagcc cgcctgctac catcaaaata acaccagtca ttccaagaga
     7081 gtgataaaga ggaacagcag ccacgacaca gagccacccc ataaaaatat agatgatcgt
     7141 agatactttt gatactctct cccgtttatg caacgtcaac gctttatata taatgccaag
     7201 aatcgcaaac aaccagatta atacaaataa agtccaacct agccatcctc ggatactcag
     7261 caaacaaaaa ggagtataac ttcctgcaat caaaagaaaa atcgaactat gatcgaatac
     7321 ttggaacacc ttttttgctt ttgtaaaaat caatgaatga aacaaagtag acgttaaaaa
     7381 taaaagaatc atcatcgatc catagatagc ataagagaca atatggaggg aggaatgcaa
     7441 gtgggcacct tttactaaaa gaaccactag tcccgcgata ctcaatccag tcccaattcc
     7501 atgggtgact gcattaaaca cttcatttaa tatttcatac gttcgattaa atttcgtttt
     7561 ttccatgaaa acggctcctt tactaggtgt aattaatgaa aaatcattat ttgtctgtta
     7621 tactaaaaat agaaattgtc ttacatgtag tttaacatga ataaggcatc ttgttaaaga
     7681 aagagagaga gaaaataatg acaaatgtaa aaatcgtaac agattcttca tgtacgatgg
     7741 aaaaaaatgt acgcgatgct ttaggcattc gtgtcatgcc gctatccatc atgatcgacg
     7801 gaacagtata tcccgatgat gaccaactag acggcgaaag atttatggaa atgatggcga
     7861 aagcaaaaaa tctgcccaaa acaagccaac caccaattgg agaattcatc tcgatgtatg
     7921 atgagctagg agaagacggc agtgaagtga tttcgatcca tatgacaaaa ggcttgagtg
     7981 gaacagtaga agcagcaaga caagccagta atctaacaaa aacgaaagta acggttatcg
     8041 acagtgatac cactgaccaa ggattatctt ttcaggtaat tcgagcagct gaattagcaa
     8101 aagaaggtaa aacagtagag aaaatccttg aagaagtcaa aaaagtacaa gacaatacaa
     8161 aattatacat cggaatatcc acactagata acttggtaaa aggtggaaga atcagccgaa
     8221 caactggttt gctgtctagt gttttgaaca tacgcgtggt gatgaatttc gcccattctg
     8281 aattaatccc tgtaactaag gggcgaggaa agaaaacgtt cgataaatgg tttaataatt
     8341 tgaaaaatga actgaaagaa ttgccaaatg tgcgacaaat cggtatttca catgcggatg
     8401 caaaagaatt ggcgcaagaa ttcaaagagg gtttgcaaga attattcccg gacatgcata
     8461 tccctgtact gcatacgaat ccggttatcg ctacgcatac tggtaaagac gcgtttgcaa
     8521 tcatgtatta tgtagattag tcgaaaagag gataagccct cagcctgaaa ggcttgcagc
     8581 ttattctctt tttctttata aaattaggaa aataattgtt ttcttttttc ataaagacta
     8641 cctttttgat aaaatttttg ataacattag aaccaatcga atacaagagg tgaaaaaatg
     8701 aaattaacga aacgacattt gatcctcgtt ttgttcccat tattaattgc gatcatcatc
     8761 tatgcaggtt tagctgcttc tataccgaaa gctggcccac taatgaaacc agtaactact
     8821 gtgagagaag atccaaataa aaaagaaaaa cttcactaca tagctatcgg agattcgctg
     8881 actgaaggag taggagatca aaccaagcaa ggaggattcg ttccccttgt cgcaaatgat
     8941 ctaaaagacc gttataattt gaccgcggtt gaaacagaaa actatggtgt aaatggtgaa
     9001 cgaagtgacc agatcttaaa gcgagtaaaa acaaaagaag aaattcgaaa caatatcaaa
     9061 acagctgatt ttatcacact gactgttggc ggtaatgatt tgatgaaagt tatccaaaat
     9121 aatttattcg ggttaacggt caattccttt aaaaagccac tgaaaaacta tcaggaaaat
     9181 gtcacagaac tattggaaga atttcgatcc ctcaataaaa ccgcacctat ttacatttta
     9241 ggcatctaca atccatttta tttgaacttt cctgagatca ctgacatgca gacaattgtt
     9301 aacaattgga acaaaggaac agaaacggtg acaaaaaaaa tgccgaactg ctactttatc
     9361 ccaatcaatg atttgcttta tcaaggattg aatcaggaag taggtgtcac agaagaagga
     9421 acagatcaaa caactagcac gacagaaaat ctaaatggca tcaaaaataa tgtgctctat
     9481 gaagaagatc atttccatcc aaataattta ggttatcaat tgatggcaaa tgcagtgaga
     9541 gatcagctga tcgagacaca aaaccaatgg ttagagaagg aggatgatca ataaacatga
     9601 aaacaagatc tgacgtatct tcaaacaaaa caaagaaaac aaattactgg aaattcgcct
     9661 tccttatcct tcttggggta gtacttggtt cgaccgtatt tttaggcagc cgtatttttg
     9721 ctaatcggga accagaactc ccagaaatac cagctcttac ggaaagacaa ggagatcctg
     9781 tcttaacgat caattcaaat aaagaaaaag tcaatcaaat catcagcttt ttccttagtg
     9841 agtatcaaaa agatagtgat attgaatata aattttattt ggaaaatgaa gcactactca
     9901 atggtacttt cgaagtacta ggttttccta tcgatttcta tttatatttt gatccgtatg
     9961 tgatggaaaa cggaaatgtc caactaaaag ctagaagtct ttctatcgga acattgagtt
    10021 tgccaatcaa agatgtgatg aatatgatca agagaaacta taaattacca gattggatcg
    10081 aagtcaatac agatgatctg actatcatgc ttaggttgga tcaattccga atgcaaaacg
    10141 ggatgtatat taaagcagat aaaatcgact tagtaaatga tgacattcgg tttagtctat
    10201 atttgccaaa agatacctct gatgcttcaa acaactcaaa taccaacaaa actagcacca
    10261 gcagtactga aagcaagaca aaagaaaata aaaaatcatc ggatgagtag tattagaggg
    10321 ggaatcataa tggaacgagc aatatttgca ggaggctgct tctggtgtat gatccagcct
    10381 ttcgacacgc ttcctggtat ccatacagtg atgtcaggat atactggagg gcatgtacca
    10441 gatccgactt atgaacaagt aaaaagccaa acgactggac atacagaagc agtggagatt
    10501 atctatgatc caacattgat ttcatatgaa caattagtag aactttactg gcagcaaact
    10561 gatccaactg acgctttcgg acaattcgag gatcgcggtg acaattatcg cccggtgatc
    10621 ttttatttga cagaagaaca gcggatcatt gctgaaaaaa gtaagaaaca gttagctgaa
    10681 agtggacgtt tcgaacagcc aatcgttaca aaaatcgaac cagcccaacc attttatcta
    10741 gcagaagaag accatcaaga gtattataaa aagcatcctg aaaattttaa aagaaaccat
    10801 gcacgccggg ctgcctttat cgaagaaaat tggaggaacg aagggtgaat cgaagctttt
    10861 ttcattattt aatgacactg cgaggcggtg gcacacatga tgctttatct gttttcgcta
    10921 cagaagccgg taaagacatc caatttccta agcacagctc tagctacgaa gaaatttcta
    10981 attatctgga actgaatgtc gactatttgc cgtccatgga tatctttgac gaagcatggg
    11041 aaaaatattt agaaaataat catgacatat aagagatagt tttgtacttt atgatcaaaa
    11101 aagttttttg tccaatagcc taattgaaag aattattttt atttcagcta tctagtcgca
    11161 tttttttgat atttttctta taataaagaa agaatgaaaa aaagagggat ggattgatag
    11221 agattgacaa aaaaacaacg ctatattaat cgcttctggc ttgtattcaa atacttactt
    11281 gtttctgtat tccttgcgtt catgttgcgg ggatttttat tcatcccggt accagtggaa
    11341 ggcaattcaa tggaaaacgt gttgaagcaa ggtgatatgg ttgtgatgga aaaattttcg
    11401 gagatcagac gatttgatat tgttgttttt caattagcag atggcacgat ttatatcaaa
    11461 cgagtaatcg gtcttccagg agaaaatgtc agctaccaaa atgatcaatt gaagatcaat
    11521 gggaaagtag ttaaggaacc ttatctaact aaaaacctga aatcagatca tgcaaatgca
    11581 tcctatacga cggactttac actacaagag ctaacgggac aatccaagct tccggaagat
    11641 aattattttg tacttggaga taatcgaaga gtttcaaaag atagtcgttc gtttggcacg
    11701 atcaataaaa ctgatattct aggtaaagca agatttgtct attatccact tgatgaaata
    11761 aaatggatcc agtgagaaac aataaagcgt gcagtctgtt gagactgcac gctttattgt
    11821 ttaatccaaa atcagtttac taccttagta cgtaaaatca aacagctcgt ttaaacactt
    11881 ttctagcctc taaagttttg ataaatgaat aaacaaccat tatttgaata gggatttgga
    11941 tcatattcct gatgaatcta cctagaaaaa ttactttgaa atctgcacca aacaaaacag
    12001 acatcagcca agaattgata aaaaggctga ctgggatcat aatcaataat tgggcagcga
    12061 tgacggaagg caggtttatc tttttatgat ataaaaataa gccattaata aatccaccaa
    12121 ggaaagccgt gataagaaaa gcagcttgaa acgttcctga accaaatatc gtgaaattta
    12181 aagtgttcga caacatttca agcaaaccag cccagatcgg tccatataga taacccataa
    12241 gcgccatact taaaaatccg aatgtgattt gaaaataagc aggaccaatc gctatcttgt
    12301 ataagatgac atcaagggct aataaaagag cagaggtcga caacaatctt gcattcattt
    12361 cgggcaatct aaaaacgtct tttgcaatca aagtgttcaa aatattctct cctttgtcag
    12421 ttaataataa aaggatacca taaaatcacc aatgtgtcat gacacattgg tgatttacat
    12481 acactggcat ctaacaatta aaaaaagggt gagaagactg cttttataaa ctgtaaaaaa
    12541 gagattgtac gaataaaaaa aatagaggcg gagaatactt atcgtatcat ccgcctttgt
    12601 ttttttgtta taaaaaacgt cagattagtt atctgcttac atcttcatcc atacgggttc
    12661 taatgtattt ccgtctagat cttggacttc taatgaaaac atttcttctt caggcattcc
    12721 catatctact tcgtggaaag taccaccatt ttcttttgca atttgagcaa atttttttac
    12781 ttcatccgca ttttcaacac taaacgaaat caaagcacca cttgtttttt tattatcagc
    12841 tatttcttta tcttttataa attttttata aaaatcatga gagagaagca ttatccaaaa
    12901 agtgtcatcc cataccattg aaccagcttc ttcagtagaa aattcttcat tttttttaaa
    12961 acccagcttt tcataaaaag caacggaacg attgacatca gcaacaggaa aattaacaaa
    13021 aaccatacta ttcatttgtt tcgtctcctt tctgttttgt tcgtctatca tctcttattt
    13081 aggaaacgct gtcaaaaatt tagattcata tatagaaacc aaaataaaaa tttgcatcat
    13141 caaaaaagtg aagatgtgat agaatgagga ctggttcact ttcacttaac taatagatgg
    13201 aatttcttta taataaaaaa gtgaatttat attgaaaaag aaaaaaacaa gtaaagccgg
    13261 ggaatattcc ggtatgaaag gaatggagag aacaatgacc atccaatggt ttccgggaca
    13321 tatggcaaaa gcccgacgag aagtttctga aaaaatgaaa tatgtggaca tcgtatttga
    13381 attggtagac gcacgattac ctttatcttc tcgtaatcca atgctagatc agatcattca
    13441 gcaaaaacct cgccttgttt tattgaataa agcggatctg gcagatccac aacagacaaa
    13501 gctgtggcaa gaatattttc gtaaacaagg ccatcttgca ttagctatca cggcacaaga
    13561 aagtaaagga attaaaggtc tagtacctgc ggctaaagaa gcattgaaag aaaaacgggc
    13621 aagagatgct gctcggggga tcaagcctcg cgctattcgg gcaatggtct taggtattcc
    13681 aaatgttggg aaatctacgt tgatgaatcg actagtaggc aagaaaattg cacagacagg
    13741 aaataagcct ggcgtaacaa aaggtcagca gtggcttcgt ttaggtaatg agttggaatt
    13801 gttagacaca ccagggattt tatggcccaa atttgaagat gaagaaatcg gcaaaaaact
    13861 agcgctgaca ggcgcgatca aagaccagct tgtccattta gatgatttag cgatttacgg
    13921 attagacttt tttgcccgat attattcagg aagaatcagc gatcgttatg gtttatcaat
    13981 cgagcaagaa gaaaaattgg caccggaatt actgatggat attacagcaa aacgtgggta
    14041 taaagaagac tatgatcgtg gcagcgaatt agtcgtgcat gatattcgtc aaggaaaact
    14101 tggacgatac acactggatc gttgcgaaga aatggagcaa aaaagtgatg actgaatcca
    14161 tccaaagtat caaagaacga ttgaaaagca tacagagaat agatgatcct acactagaat
    14221 cattaagaaa tgatccacgc aaaggcgtgc agcaagcact ggcttctttt gaacgacgat
    14281 tgaaaaagga agcattaatt aaggaaaaat atgaagaaat gttttgcttt gaaaagcaag
    14341 ggtacgcaca aggtcaccaa tggattgccg gcattgatga agtaggaaga ggaccattag
    14401 ctggacctgt cgtggcagct gcagttatct tgccgaaaaa tgagatcata ttagggctga
    14461 atgactccaa acaattatca gagaaaaagc gagaacttct ttatcaacaa gttcaagaaa
    14521 aagccctggc aatcgggatc gggatcgttg atcagcagac aatcgatgat gtgaatattt
    14581 atgaagcaag taaattagcg atgatccaag cagtagaaaa attgtccatt gtaccaagct
    14641 atttgttgat cgatgcgatg accttggatt tgcctattca gcaggaaaaa ataatcaaag
    14701 gggatgcacg atctgtgtcg attgctgctg caagtatcat tgctaaagta tatcgagacc
    14761 aattgatgaa agaatatgac gtgctttatc ctggatatgg tttctcgcat aatgcaggtt
    14821 atggaacaaa ggaacactta gaaggacttg aacgacaagg aattacgcca atccatcgta
    14881 aaagttttgc acctgtaaaa aaatatttac aataaaaaac agccgctttt acgtatctta
    14941 ttagaaagag agacgaaagg cggttttatg atgtatcaaa tagaagaaaa tttattgaaa
    15001 cttgcttata caaaaggaat cagtagtcaa ggaaaatggg cacttgctaa ctggatgatg
    15061 cagtatcctt ataaagaaat cggatttgaa gacatcatta agataggtaa aataaccaaa
    15121 caccgggagc tgtttcgcaa aagttggcag gaaattcacg ctaactgggg caaaatccaa
    15181 tcgaaacagt cgtttatcac ttgctttgat cccaaatatc cccctcagct gcttcacttg
    15241 acctatccgc caattgtact gttttataat ggtgatctat ctttactttc ttgtaacatg
    15301 ttaagcgttg ttggtggaag acacacttct gctttagcca aaaaaacagt ttattattta
    15361 ctaaaaccag tgatagaagc tggatatgtg atcgtcagtg gctgcgcgaa agggatagat
    15421 acatacggtc accaagcagc aatctctcat ggtggacgga caatcgcagt cattggaaca
    15481 gggatagagc aagtctatcc tgctgaaaat cgtgacttgc aagaggtgat tgcaaatgat
    15541 catcttcttt tgagtgaata tcctcctcaa tccggcccga aaaagcattg ttttccaatg
    15601 agaaatcgga ttatcgcagc tttgtctaaa ggaacatgtg tctttgaagc caagataaaa
    15661 agcggctctc tgataacagc acagcaagca ttggatcttg gaagaacagt tttttctgtt
    15721 ccaggaaata ttttaacaga acattctacc ggatgtcatc atttgatcca agacggtgca
    15781 gtttgcacgg tttcgggtga agatatcttg gcagaattta aagaataaca ccatactttt
    15841 catttgcttt tcttgacaaa cgttttctga atggatatga ttagctacga tttgtatgaa
    15901 tcctctttta atagtaggaa cacgacaatg gaaaggagtc cattcagtaa tggcgtataa
    15961 atatttagtg attgtagaat cacctgcaaa agccaagaca attgagaaat atcttggacg
    16021 aaactataaa gtaatggcca gtgttggcca tataagagat ttaccgaaaa gtaaaatggg
    16081 tatcgatttt gaaaacaatt atgaacccca ttatatttct atacgcggaa aaggcgatgt
    16141 catcaaaagc ctgaaagccg cagcaaaaaa agctcaaaaa gtttacttgg caagtgaccc
    16201 ggatagagaa ggagaagcga ttgcttggca tttagcgtac ctacttgggt tggatctgaa
    16261 agaaaaaaat cgggtggtct tcaatgaaat cacaaaagac gcagtcaaag cagcttttaa
    16321 ggaaccaaga acgatcgatg tagatttagt agatgcacag caagctcgtc gtaccttaga
    16381 cagaatcgtt ggttattcga tcagtcctat tctctggcgt aaggtcaaga aagggttaag
    16441 tgcaggacgt gtccaatctg tcgctttaaa aattattatt gaccgtgaaa aagagatccg
    16501 agaatttgtt ccagaagaat attggagcat cgacggtaat tttaaaaaag ctcgcaagaa
    16561 attcaaagca aatttctggg gaatcgacgg taagaaaaag aaattaccag atgcacaaag
    16621 tgtaaaagaa gtcactgcta gaattgatgg aacagacttc gaagttaaaa aagtagaaaa
    16681 gaaagagcgc aaaagaaacc ctgcgaatcc tttcactaca agtagcctgc aacaagaagc
    16741 tgcccgtaag ttaaactttc gaacaagaaa aaccatgatg gtagcacagc aattatatga
    16801 aggaatcact ttgggtaaac aaggaacagt cggcttgatt acatacatgc gtactgattc
    16861 tacccggatt gccgatacag caaaagcaga ggccgcagca tttattgaag atacgtatgg
    16921 aaaagaattt tctagacatg atcaccgaaa gatcaaaaat gctcaaggcg cacaagatgc
    16981 ccatgaagct gtgcgtccaa cgagtgtgct tcgtacacca gatgaactca aacaatattt
    17041 agataaagat caattgaaac tttatacatt gatctggtca cgttttgtgg ctagtcagat
    17101 gacaccagct attttagata caatgaaagt gacattagaa caaaatggtg tcatcttcat
    17161 tgcgaacggt tcaaaaatca aattcaaagg attcatgcaa gtgtatgtgg aaggtagaga
    17221 tgacggcaaa gaagagaaag aaaatattct tccagaactt gaagaaggcg atgtagtcca
    17281 atctgttgat attgaaccaa aacaacattt tactcaacca ccagcacgtt ttagtgaggc
    17341 tacgttgatt cgtgcactag aagaaaacgg ggtagggcga ccttccactt atgcaccaac
    17401 acttgatacg atccaaagac gctactatgt aaaattgaca caaaaaagat ttgagccgac
    17461 tgaactagga gaaattgtca attcattaat ctgtgaattc ttccctcaaa tcgtcgatat
    17521 ccatttcaca gcagaaatgg aaggagattt ggataaaatt gaagaaggca cggaagcgtg
    17581 ggtcaaagtg gttgatcgtt tctataaacc gtttgaaaaa gaactgacga atgcggaaga
    17641 aaaaatcgaa aaaattcaaa tcaaagatga accagcagga ttcgattgtg atgtctgtgg
    17701 acatccgatg gtgataaaat tagggaaata tgggaagttt tatgcttgca gtaattttcc
    17761 tgattgtcgg aatacgaaac caatcgtaaa agaaatcggt gtgacttgcc cagtatgtca
    17821 tgaaggacaa gtcattgaac gaaaatctaa gaaaaacaga ctattctatg gttgttcccg
    17881 tttcccagaa tgtgaattca cttcttggga taaaccagtg ggcagaaatt gtccaaaatg
    17941 tgacggatat ctggtcgaga agaaagtcaa aggcggtaaa caagtagtct gtgtaaatgg
    18001 agattacgaa gaagacatcc aaaaataata gaaaagaggt tctttcattg aaaatggtaa
    18061 atgttgttgg agcagggctt gccggaagtg aagctgcttg gcagatcgca caagcaggag
    18121 tccctgtccg tttatatgaa atgcgtccag tcaaatcgac tgaggcgcat cacacaagta
    18181 attttgctga actagtatgt tccaattcat taagaggaaa cagtttggct aatgcagtag
    18241 ggttattgaa agaagaaatg cgccgattga attcggtagt aatcacttcg gcagatgaaa
    18301 cagcagtgcc agctggtggt gccttagctg ttgaccgcga ttctttttct gaaacaatta
    18361 cccgaaaagt caaagagcat ccattagtaa cagtgataaa cgaagaaatt actgaaatac
    18421 cggaaggaat caccgtagta gcaacaggac ctttgacttc tcatccatta gcagaaagca
    18481 tcaaagcatt caatggttca gatggttttt atttctatga tgcagctgca ccaatcgtag
    18541 acaaagctac gatcgatatg gataaagtat atttgaagtc ccgctatgac aaaggagaag
    18601 cagcttactt gaactgtccg atgacagaag aggaattcaa agctttccat gaggcactaa
    18661 tcaatgccga agtggcagaa cttaaatctt ttgagaaaga aaaatatttt gaaggatgta
    18721 tgccaattga agtaatggca cagcgaggat tcaaaacgat gcttttcgga cctatgaaac
    18781 cagttggatt ggaagatcct aaaactggca aacgtcctta tgctgttatc cagctgcgtc
    18841 aagacaatgc tgcagcttct ttatacaaca tcgtgggctt tcagactcac ttgaaatggg
    18901 gcgagcaaaa acgtgttttc cggatgattc ctgggttaga aaatgctgaa ttcgttcgct
    18961 atggtgtgat gcaccgaaac agctttatga attcgccaga attgctgaaa ccgacttacc
    19021 aatcaacgaa acgcgaagat ctgttctttg caggtcagat gactggtgta gaaggctatg
    19081 ttgaaagtgc agcgagcgga ctacttgctg gaatcaacgc cgcacgctta gcaaaagaaa
    19141 tggaaccagt cgagtttcca caagaaacag ctattggaag tatggcttac tacattactc
    19201 atgcggaagg aaaacatttc caaccgatga atgccaacta tggtctgttt cctgaactgc
    19261 cagagcgcat tcgagacaaa aaagcaagat atgaagcaat tgcacaacga gctttaaaag
    19321 ctaatgaggc aataaaaaca gaattagaaa aaactgcaat cccaaatgag taaggaaact
    19381 ccatgaagag ggtgagacgg atagtctcgc tctttttctt gcgtttttct taagaatttc
    19441 tcaaacaaaa tgtgaggaat tgcgccaatt gtgaacaaac ttagaaatat tggttaaatt
    19501 ctgacaattt atttgtcttt ttataaacgc tgtgctaaat tattctctgg gacaaaagga
    19561 aggtgagaaa atgacagaag ccacgtggga acaaaaattt ttgcgttatt tgattgtcga
    19621 aagaggatat tcagaaaaaa cgaaagaagc atatgaagaa gacattctcc atttcaaaca
    19681 ttttttggag gaatctggag acgctgatct gctgaaggcg gaatatttta cggttcgtgt
    19741 ctatttgagt gcattagctg atcatcgtcc tagctatagc agaaattcta tcagtcgcaa
    19801 gatagccagc ctacgttcgt tttatcagta tcttctcaaa gaagaagtga taaaagaaaa
    19861 tccattttct tacgttcatc tcaaaaagaa aaatttgaga ctgccacgtt ttttttatga
    19921 aaatgaaatg caagcacttt ttgacagcgt agcaggagat agtcctttgg atttgaggaa
    19981 ccgcgcttta ctggaagtcc tttacgggtc aggcatccgt ttaagtgagt gtagcagttt
    20041 gtcggtatca gacattgatt ttgattcaga ggtcatgttg atccatggta aagggaacaa
    20101 agagagatat gctcctctag gttcttttgc tcaagatgct ctgcaagaat atttcaggga
    20161 aggccgccag gtattgatga caaaatatca cgaagagcat gatatcgttt ttgtgaatca
    20221 tcatggaaaa ccaatcacgc ctactggaat tgaatatgtc ttgaatcaaa tgatcaaaaa
    20281 aagcagtttg gatagtgata tccatcctca catgctgcgc catacatttg ctactcattt
    20341 attaaataat ggggcagatt tgcggacagt ccaagaattg ctaggacatg ccaatttatc
    20401 gactacccag atctatgccc atgtaacaaa agaaagttta caaaaaaact atcgttcttt
    20461 ccatccaaga gcgtaaaaat aaggaaaaga acaaatttag gaggaaacaa acatggtcga
    20521 atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat ttgctatggc
    20581 tggagatggc caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg caaagaaagt
    20641 acgacgtatc tacaatggag aagttgttgt aggtttcgcc ggaagtgttg cagatgcgtt
    20701 tactttagaa gaaaaatttg aaggcaaact aaacgaatat aacgggaatc tacaacgcgc
    20761 agctgtcgaa ttagctcaag aatggcggac acagcaatct atgcaaaaac ttgaagcgat
    20821 gctgatcgta atgaatgata aagaaatgct tcttgtttca ggtacaggag aagttatcac
    20881 accagatgat ggtatcttag ctatcggatc aggagggaat tttgcgttgg ctgcagcccg
    20941 agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa gaaattgcag aaaatgcgtt
    21001 aaatattgcc gcagatatct gtatctttac taatcataat attatcgtag aagaactgta
    21061 agaggtgagt agatatgtat gaattaaaac aaacaccaag acaaatcgta agtgaattag
    21121 atgaatatat cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct ttgcgtaacc
    21181 gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga tattacacca aaaaatatgc
    21241 tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg gcaaaaatcg
    21301 tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt tatgtcggaa
    21361 gagatgtaga atcaatggtt cgggatttag tagaaaatgc gatccaaatc gtagaaaaag
    21421 agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt ttggtaaaaa
    21481 tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt gaacaaatga
    21541 tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa gtgacagaag
    21601 atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta ctggatagtc
    21661 gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg aacaatggat
    21721 tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg aagaaaaaag
    21781 tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa gaatcagcaa
    21841 aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa gaaagtggaa
    21901 ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct ggagaagtat
    21961 ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa gtaaacacaa
    22021 aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct ttccatttgt
    22081 ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg gtagaattag
    22141 atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat gctttagtaa
    22201 aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca aaagaagcaa
    22261 ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat atcggcgcac
    22321 gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa gcaccagata
    22381 tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt ggctcaattg
    22441 cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga caatgacaga
    22501 cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata cttttgacat
    22561 gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc ttgacagcaa
    22621 cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc acgatgtgaa
    22681 caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt atactgatac
    22741 agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata ttactgcatt
    22801 tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc cgatctttgg
    22861 agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt ttgcagatga
    22921 cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc tttatcagaa
    22981 gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg caatcaacac
    23041 attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg atggagatga
    23101 aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt ctgtgatcgt
    23161 caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt taggcatgaa
    23221 gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa aacacagtta
    23281 ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc attttaaaaa
    23341 gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc agctgatcgc
    23401 cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac atggtttaga
    23461 gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt tattcccttt
    23521 tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc aaatggggca
    23581 acacggattt gctcgggata tggattttga agtcattgaa catggacaag aaatggctag
    23641 tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt ttgaattagt
    23701 gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg caaataccgg
    23761 tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac ctcttgaaga
    23821 aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa gattacgcct
    23881 accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga caaatacgaa
    23941 tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa caaggggtct
    24001 aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa gctataaaga
    24061 tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg tttgtatcga
    24121 accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg aaaaagtagg
    24181 gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca cagtgaagta
    24241 agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt aaaaaagcta
    24301 tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt atctctttgg
    24361 aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga ttcgactttc
    24421 agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa aggaagaaac
    24481 taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag gagctacata
    24541 aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc tcaaactcac
    24601 catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa aaaatacagg
    24661 attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt tgaacccagc
    24721 aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag ggttaatccc
    24781 agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa tatccataag
    24841 caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg ttccagtatt
    24901 accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc agacaccgga
    24961 aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca ttttttaccc
    25021 tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga acaattgaaa
    25081 aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt ggcagaaatg
    25141 tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc aaaaaatatt
    25201 gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc acaggtattg
    25261 caagacaatg gatataaaat cataccagta aatccattat tggctggtga agaaattcta
    25321 ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat cgtagatgta
    25381 tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac tgatgctaaa
    25441 gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct aagaaaagca
    25501 ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa gttgaaagaa
    25561 aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat gtcgaaaaag
    25621 cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca cgaccttttc
    25681 tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc gtttgccttg
    25741 gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga aggattagag
    25801 gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg cttgcatcat
    25861 ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta tggcagtgag
    25921 attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg acgtgggatg
    25981 cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac tgtgctgcat
    26041 gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca cggtgtagga
    26101 gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag agatggcgta
    26161 gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa aaagatagga
    26221 aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac aatattcagt
    26281 acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc ctttcttcta
    26341 aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa ggaaattttt
    26401 cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa agatacattg
    26461 acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt cgcttatcaa
    26521 tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg gacaaaagat
    26581 gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa tgaatatgcg
    26641 agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga tttcagagaa
    26701 gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt tgaagggcaa
    26761 acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt catcagtgaa
    26821 caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt ccgtaaagca
    26881 atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag ccgaaatggg
    26941 aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc acaatcccga
    27001 aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg atctgctaaa
    27061 caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt catcaatacc
    27121 gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat gatctatacg
    27181 attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga caaagtaatc
    27241 atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct tactttcttt
    27301 tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt accgccttta
    27361 tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg gacagatgac
    27421 gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg atataaagga
    27481 cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac ttcccgtacg
    27541 ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac cacactgatg
    27601 ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt tacgctcgaa
    27661 gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc agtttctaat
    27721 gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt tgaagtagaa
    27781 taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta gaagaagtga
    27841 tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca ctgcctgata
    27901 tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac aaagatggca
    27961 atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc atggggaatt
    28021 atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc caagactgga
    28081 agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac ggtgatccac
    28141 ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag atgctagcag
    28201 atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa aaagaaccaa
    28261 cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt atatctgctg
    28321 gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc acaatctata
    28381 tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct ggacctgact
    28441 ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat gaaacaggac
    28501 gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt aataaacagc
    28561 aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg aaaaaaatgg
    28621 atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat gaaagtgata
    28681 gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa ggaattttga
    28741 attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg gtcgctatcg
    28801 accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat atcgagcatc
    28861 gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa aaaagacagc
    28921 atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc gcaacgattc
    28981 gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt caatttacag
    29041 aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac acggatatca
    29101 cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg aataaaatcc
    29161 tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa gtgaaaaaaa
    29221 attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc aaaattgata
    29281 cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa ggctatgtga
    29341 agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg atgcgagaag
    29401 gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt ctgatcacaa
    29461 ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa tggaaagatg
    29521 ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg attttagctg
    29581 ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc aagaatggat
    29641 tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag agccgtccgc
    29701 tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat ctgaaagaag
    29761 atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg cgctatcctt
    29821 taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg aatttgaaag
    29881 atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg ccgatcgtaa
    29941 tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc caaacttcac
    30001 gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac cgtattatct
    30061 atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg cagcagttaa
    30121 cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca tttgcgatgg
    30181 atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt ttcatcgagt
    30241 cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag gtggcgtctc
    30301 tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa agtgttcaca
    30361 atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa cataaaaatt
    30421 gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata ctaatcacgt
    30481 aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa tggaatggat
    30541 ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa aataactaca
    30601 ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca gaaaaattat
    30661 gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc tatgatatgg
    30721 acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat aaagatttgg
    30781 aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg ccgttcggcg
    30841 gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac gatgaattga
    30901 cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac gcttatactg
    30961 ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac gcttacggtc
    31021 gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac tatttgatgg
    31081 aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa gaagtgattc
    31141 gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa gaaatggctg
    31201 cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct gtccaatggc
    31261 tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg tcaatcggac
    31321 gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga gtaatcacag
    31381 aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg gtcaaattcg
    31441 cgcgtacacc tgaatataac caattattct caggattccc tatctgggct actttatcca
    31501 ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc cgtctgcttc
    31561 atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg tattctgaac
    31621 accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt tcttcgattc
    31681 aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt gcgattgctt
    31741 gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct cgtgctaact
    31801 tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact ggcgcccaag
    31861 ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa gtgaaacata
    31921 acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg aacatcattc
    31981 attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg gattctgact
    32041 tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac agcttgtcag
    32101 cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca gttgattttg
    32161 aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat gaattagttt
    32221 catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac cgcaactcaa
    32281 aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa aatacaggaa
    32341 atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat ccaagttaca
    32401 atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg aattacggct
    32461 gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt ggaaaagaac
    32521 aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca aaaggagctt
    32581 atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg gaaaatccaa
    32641 tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc cgtgacttga
    32701 caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg taaaaagttc
    32761 gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg catcaaaatc
    32821 gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt gtatgttccg
    32881 tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt cagaaagctt
    32941 cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga acttaaatgg
    33001 aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta gacggacctg
    33061 gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt tgtcacaatc
    33121 cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt ttagataaag
    33181 cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt ggtggagaac
    33241 ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag cggaagatgc
    33301 atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc ttttcacgtt
    33361 ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac atcgataatg
    33421 aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg gctcaatatt
    33481 tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag cgaagtgatt
    33541 atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat gtagacagag
    33601 tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt ggtttgaaat
    33661 atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct aaacgattgc
    33721 ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa agaagataag
    33781 tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa ggagcgaatg
    33841 acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca atcggggcag
    33901 ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca gttgctctag
    33961 gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct gcaccacgag
    34021 tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat aatgaattcc
    34081 aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac caccatcgta
    34141 ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt ggatgtacaa
    34201 gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa gaaatcgcag
    34261 gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca acatgtacgc
    34321 ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat ctaaacactt
    34381 atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct gcggttcttc
    34441 ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc gctcaagtca
    34501 atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca gcaatggaag
    34561 atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac atcttagaca
    34621 gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa gctttccaaa
    34681 caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa aaacaagtcg
    34741 tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat tcaaaatgaa
    34801 tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa gaaatggagg
    34861 agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag ccttttaagt
    34921 aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa taaggaaaac
    34981 ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta tctatatgaa
    35041 catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca aaataaattt
    35101 tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga acgaagatat
    35161 catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac ttcggctgct
    35221 gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt agcgatggga
    35281 gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt ttatggagta
    35341 agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa gtaccgtttt
    35401 atcacacttt cctctataga acacttcccg cttactttgg ctaattactt ttttgtacag
    35461 aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat tggagacgta
    35521 aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg taagaataaa
    35581 tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa tactataaag
    35641 gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt tgagattgaa
    35701 ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa acgacaaatc
    35761 ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa agaatatgga
    35821 acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg actcttgcag
    35881 gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg cctatctacc
    35941 acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg gtgagaaatg
    36001 tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc aatattatga
    36061 ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga agtccatttg
    36121 ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc aatcttgggt
    36181 tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg taatagtcag
    36241 gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatac acctagactg
    36301 acgatgaaga gcatatccaa cagatttcct gcgacttgga tcgtcgaagg gttggtcagt
    36361 ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt gacaggaact
    36421 aatcgaaaga agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat aaaataagta
    36481 aaaatggata aaatcacgac taaaccattc atgataaaga agaaaagcgt attgtgatta
    36541 aacgaataaa aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa tacgcttgaa
    36601 gcttcgttta acgtaccagg atgtcgatga aaaaaatagg caccgaaata aacagaaaac
    36661 agtaagccgc aaagagaaga caaaatcaaa aaaaacggat tttcttcggc ttctgtgata
    36721 tcagaatgat ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga ggtcttaagg
    36781 tatgaataac atagaaaaaa taa
//
LOCUS       DAZAFF010000016        35423 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000016 DAZAFF010000000
VERSION     DAZAFF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.016"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..1021
                     /locus_tag="ACG9BB_000822"
     CDS             56..1021
                     /locus_tag="ACG9BB_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFN2836789.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(1078..1308)
                     /locus_tag="ACG9BB_000823"
     CDS             complement(1078..1308)
                     /locus_tag="ACG9BB_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFN2836790.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1394..2281)
                     /locus_tag="ACG9BB_000824"
     CDS             complement(1394..2281)
                     /locus_tag="ACG9BB_000824"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN2836791.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(2370..3593)
                     /locus_tag="ACG9BB_000825"
     CDS             complement(2370..3593)
                     /locus_tag="ACG9BB_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN2836792.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(3610..4656)
                     /locus_tag="ACG9BB_000826"
     CDS             complement(3610..4656)
                     /locus_tag="ACG9BB_000826"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFN2836793.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(4820..6547)
                     /gene="ptsP"
                     /locus_tag="ACG9BB_000827"
     CDS             complement(4820..6547)
                     /gene="ptsP"
                     /locus_tag="ACG9BB_000827"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFN2836794.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(6547..6813)
                     /locus_tag="ACG9BB_000828"
     CDS             complement(6547..6813)
                     /locus_tag="ACG9BB_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFN2836795.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            7292..9526
                     /locus_tag="ACG9BB_000829"
     CDS             7292..9526
                     /locus_tag="ACG9BB_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2836796.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            9674..9994
                     /locus_tag="ACG9BB_000830"
     CDS             9674..9994
                     /locus_tag="ACG9BB_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFN2836797.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(10199..10423)
                     /locus_tag="ACG9BB_000831"
     CDS             complement(10199..10423)
                     /locus_tag="ACG9BB_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFN2836798.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(10621..12201)
                     /locus_tag="ACG9BB_000832"
     CDS             complement(10621..12201)
                     /locus_tag="ACG9BB_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFN2836799.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(12602..13978)
                     /locus_tag="ACG9BB_000833"
     CDS             complement(12602..13978)
                     /locus_tag="ACG9BB_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFN2836800.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(14106..15194)
                     /locus_tag="ACG9BB_000834"
     CDS             complement(14106..15194)
                     /locus_tag="ACG9BB_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN2836801.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            15366..15800
                     /locus_tag="ACG9BB_000835"
     CDS             15366..15800
                     /locus_tag="ACG9BB_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2836802.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(15878..16411)
                     /locus_tag="ACG9BB_000836"
     CDS             complement(15878..16411)
                     /locus_tag="ACG9BB_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFN2836803.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(16505..17683)
                     /gene="mutY"
                     /locus_tag="ACG9BB_000837"
     CDS             complement(16505..17683)
                     /gene="mutY"
                     /locus_tag="ACG9BB_000837"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFN2836804.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(17676..18473)
                     /gene="recX"
                     /locus_tag="ACG9BB_000838"
     CDS             complement(17676..18473)
                     /gene="recX"
                     /locus_tag="ACG9BB_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFN2836805.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            18580..19962
                     /gene="rlmD"
                     /locus_tag="ACG9BB_000839"
     CDS             18580..19962
                     /gene="rlmD"
                     /locus_tag="ACG9BB_000839"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFN2836806.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(20136..20714)
                     /locus_tag="ACG9BB_000840"
     CDS             complement(20136..20714)
                     /locus_tag="ACG9BB_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFN2836807.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            21036..21506
                     /locus_tag="ACG9BB_000841"
     CDS             21036..21506
                     /locus_tag="ACG9BB_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFN2836808.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(21614..22840)
                     /locus_tag="ACG9BB_000842"
     CDS             complement(21614..22840)
                     /locus_tag="ACG9BB_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN2836809.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(23594..23794)
                     /locus_tag="ACG9BB_000843"
     CDS             complement(23594..23794)
                     /locus_tag="ACG9BB_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN2836810.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(23928..24536)
                     /locus_tag="ACG9BB_000844"
     CDS             complement(23928..24536)
                     /locus_tag="ACG9BB_000844"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFN2836811.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(24621..25091)
                     /locus_tag="ACG9BB_000845"
     CDS             complement(24621..25091)
                     /locus_tag="ACG9BB_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFN2836812.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(25139..26053)
                     /locus_tag="ACG9BB_000846"
     CDS             complement(25139..26053)
                     /locus_tag="ACG9BB_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN2836813.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            26199..27002
                     /locus_tag="ACG9BB_000847"
     CDS             26199..27002
                     /locus_tag="ACG9BB_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFN2836814.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            27180..28127
                     /locus_tag="ACG9BB_000848"
     CDS             27180..28127
                     /locus_tag="ACG9BB_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFN2836815.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(28195..28488)
                     /locus_tag="ACG9BB_000849"
     CDS             complement(28195..28488)
                     /locus_tag="ACG9BB_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFN2836816.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(28516..29181)
                     /locus_tag="ACG9BB_000850"
     CDS             complement(28516..29181)
                     /locus_tag="ACG9BB_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFN2836817.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(29321..29953)
                     /locus_tag="ACG9BB_000851"
     CDS             complement(29321..29953)
                     /locus_tag="ACG9BB_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN2836818.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(29960..31027)
                     /gene="liaS"
                     /locus_tag="ACG9BB_000852"
     CDS             complement(29960..31027)
                     /gene="liaS"
                     /locus_tag="ACG9BB_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFN2836819.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(31024..31755)
                     /gene="liaF"
                     /locus_tag="ACG9BB_000853"
     CDS             complement(31024..31755)
                     /gene="liaF"
                     /locus_tag="ACG9BB_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFN2836820.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(31924..32403)
                     /gene="greA"
                     /locus_tag="ACG9BB_000854"
     CDS             complement(31924..32403)
                     /gene="greA"
                     /locus_tag="ACG9BB_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFN2836821.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(32539..33714)
                     /gene="mltG"
                     /locus_tag="ACG9BB_000855"
     CDS             complement(32539..33714)
                     /gene="mltG"
                     /locus_tag="ACG9BB_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFN2836822.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            33918..35087
                     /locus_tag="ACG9BB_000856"
     CDS             33918..35087
                     /locus_tag="ACG9BB_000856"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN2836823.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT        10673 a         7139 c         5860 g        11751 t
ORIGIN      
        1 ccattatttc aaaatttgtt atagtctttt tattcaaagc gtaaaaggag atcctatgtt
       61 aaaagaaaaa atgaaaactc tccccatgct gccgggtgtc tatcttatga aagaccgaga
      121 tggtaaggtc atctatgttg gtaaagcaaa acggctcaaa aatagagtaa gcagctattt
      181 tcatcagaat aaacagcatt caaaaaaagt attacgtatg attcatcata taacagattt
      241 tgatttcgtc gtagtagaca cggaattaga tgctttattg ctcgagtgtc aattgatcca
      301 acattaccgt cctttttata accgacaaat gaattatttc agtaattata attatgtaca
      361 tattaccaat aagggatttg tattaacaga tactcctacg gctcgaacat acgggccttt
      421 tcgtctctat aaaaaaatgc cgtctatttt gcgtataatg gaagaaactt atcaaatgcc
      481 ttggttgtct gaaattagtt tgttagcttt gcgagtccag cttcctgacc tacaggaaat
      541 gacttttgaa cagaaaaaaa aggaactcca agggctgttt caaggccgaa ataaaaagct
      601 actgacttac ttgaaaaaaa gacaacaaca tttcatctat cagttgaatt tcgaaaaagc
      661 cggtatgctt caaaaagata tcgaattagt tacttatttt attagacgga tccaggaaca
      721 aaagcagttt ttaagaacac catcgctcac cttcagcatg ccattagcag cagatgagag
      781 tcagaaaaag cactacctcg tttgttatgg tcaacttgca gaaacaatga ttacctctgg
      841 tgacgtttct cctgacttct attatgaaaa gaaagaagct catttatctt tgaaacgtca
      901 attgtcaaaa gaagaaatcg atcctgtaca aatcttgatc agttaccgca aaaaattaga
      961 aaaagaacag tttgaaattg aactgttaaa caaaaaagaa gctgaaaagc aactaaatta
     1021 gttgcttctc ggcttctttt aggcatcttc aacttcaaat gtcaattatc ctaaaattta
     1081 ttggattaag tcatagattt ccatcgctac gatatcgata ttatcaaatt cgtattgctg
     1141 atgagtatct gtttcagtta attcaaatgt atcggtcgct ttatcataag ttactgtaca
     1201 cttctctacg ccttcttttt caaaacggcg aacttgcagt tcattatctg ctgactcgac
     1261 catagcctcc agtcgtttta tgattgcgac taattgtgaa tgtgtcatcg gaggtcctcc
     1321 ttttcttatt tctgttatat tgtatcaaaa gcatagccta cttgcattga ttttctctaa
     1381 aaaaactcgt tttttatgat tttttttctg gtatttttcc ttcaggccac caaagtttga
     1441 tcaaagcttg ggttccgtca ttctcaaaac ctttatctgc caactcttca tatagtttca
     1501 atgcttgggt agttgctggc aatacaagat ccattttttc tgcttcttct aatgcaatct
     1561 ttaaatcttt gataaaatgt ttgacaaaaa aaccaggtgt gtaatcttct tttaaaatac
     1621 gaggagaata attgcttaaa gaccagttag ctgcacttcc acctgaaagc gtctcaatca
     1681 ctttctttaa atcaagtccg tttttttgtg catagacaag catttccgtc attcctgtca
     1741 tcgtacctgc aatcatgatc tgattagcca tttttgtatg ctgacctttc ccagcagaac
     1801 catgcaatgt aaatgtcgta ccaaattctt tgaataaagg cagtacttga tcataaacgg
     1861 cttcttctcc accgaccatg atcgtcagtg taccattctt tgctcctaag tcaccaccgg
     1921 ataccggtgc atctaacgca tctgcgcctt tttcttttgc tgtttttgca attttttctg
     1981 ccaggcttgg tgtacttgta gtcaaatcaa ctaaaatatg tcctgagata tctgcagaaa
     2041 aaatccccga ctgtccatag tagatttctt ctacatcttt ggggtaacct accattgtaa
     2101 agagaatatt actttgttct gctaccgctt ttggtgtatc tgcccagata gctccttctg
     2161 ttactaaagg atcagctttt gcttttgtcc gattatagac agctacttcg tgtcctgctt
     2221 ctagtagatg gcgtgcgaca gcacttccca tgacgcctgt tccgataaaa ccaagtttca
     2281 ttttttcgac ctcctaaaaa tcaaaaatcc ttgcttctat gctagtatac gctagaaaca
     2341 aggatttttt cataaaaaat gtttgaattt tatcttttaa atgaatttaa ttttattttg
     2401 attcgttcaa tcgagtcttc ttcttctttt cttcgcatct cttcttcatg ataaacaacc
     2461 atgtcttgat aaaattcgat catagcttcg ccgaaatgtg tagatgaaat atcataaagt
     2521 ttagcttcta aaacttttgg atctttagga acttcactgg aaatataatc aataagtgct
     2581 ggtgcaaagt cttcgtcggt ttcaaaagtt ttccctaatg aaggatgatc gaacaatcgg
     2641 ttcaaataag catttccttc cacgacacaa ggtgttccgg ctgccatcgc ttctgtataa
     2701 gtaagccctt gtgtttctga agtagaagca ctgacaaaat aatctgccgc tttataataa
     2761 agtgctacat cattgttgtc cacttctcct gtgaactgca caaattcttc taactgcagt
     2821 tctgagatca attcctctaa ctcttctaca taaggtcctt ttcctacgat aactaatctg
     2881 gcgttaggat aagcggagat gacctgagga aacccattaa tcaaggcttg gatatttttc
     2941 tcataagaaa tcctgcttaa agaaagaatc atcaaatggt tttcctgaag acctagtgac
     3001 tcccttaatt tgctgatatc ctgctgagtg atatcagaac gtttgaattt ttcaatgtcg
     3061 ataccagtag gaatcacgcg aagcggtgct attacaccat agctgcgtaa tgtatcaata
     3121 acacgttcac ttggacaaac tacacctgtc gtatgatttg caaataatct agacaagtat
     3181 tttacatggg attgcctgat cacttttcct ttaccgatat aatgaaggta gtcttcatac
     3241 atcgtatggt aagtatggat cagaggtatt ttcaacttct tagctaccat cttcccaagt
     3301 atccctgctc cgaattctgt atgggtatga ataagatcta gttctagttc tttggcaacc
     3361 aaataagcat accacatgcc tctaacaaca actcttcgat ccttgaaaga aataaatggt
     3421 acacttggca ttcgaatcac atctttttcc aacttatccg cattaggatc ggtcgttgta
     3481 aaaatataga cttcatgccc atgtctttct aattcttgtt ttaatgtttt gatcgatgta
     3541 gcaactccgc tgacttgcgg aaagtaggta tcggtaaaaa agccgatttt catgtttttc
     3601 gcctccaaac tagtccttga cctgatttac tttttttaat ttgctagaga ctttttcatt
     3661 cagtacactt tcgtatacat ttttaagttc ttcaccgatt ttattgatac ttcgctcttt
     3721 tgctgtttga taaccggctt gtgtcgtact cggtagttct tgattggtaa tctgttggat
     3781 cagtcggata aattcttcgt ttgtcttccc catatagcag ttcttgctat cttctaacca
     3841 gccattatac actggaatat ctcgaacaag tacgttttgt ttgcttgcta aagcttctaa
     3901 aacaacgatc ccttctgtct cttcataaga agggaaaaag aatagatctg cacctgaata
     3961 cgcaccttct atgatctctc cacgaatgta accaggaaac aggacatttt caggatgatc
     4021 ttcttttaca attttccgga tgttccgagg aatcgagtac atgggaacat gaccaaacca
     4081 aataaatgtg tattcgggca tcttcttagc aatttctacg aaatcaatga ttcctttacg
     4141 ttcgaaaaac aaaccgacac aaatgattac tttttgatcc ggagacagtt tgaaatattc
     4201 tctgaacttt tgttctttca ttggatcagg gtgatatttc tctaagtcga tcccattgga
     4261 aatagcttga atcggcacac ggataccata gctttccaac aactgttttg aataaggtgt
     4321 tggcgtgatc agatagtccg ctttttggta caatcctacc agatattttt ttacgatagg
     4381 agaaagctgg ttcgatctga taaaggaatt tcggaaatct tcctctgtag aatgcgcatg
     4441 atagacaact ttttttcctt ctcttctggc tttattgacc atattatgac tattgattcc
     4501 gtatgtattg atgtgcagga tgtcataatc ttctttttca tctagtgtat aagagatacc
     4561 gacttccgtc agagcacgtt tttgatgatc aagcgcacgc ccgattccag attttgcaag
     4621 catcttttca ctttcaaaat ataataatac cttcacctta cgtccccctt cacttatata
     4681 gtataacata gcctacaaac tgtcactcaa acttaagccc tatttttgtc tattcttact
     4741 gacaaaaacg ttttcttaaa aaagaacaaa aaaactggcg tagctctttt acaagccaca
     4801 ccagcttttg atacgttgtt tattttacat attcatgaac aagttcaacg acttcttcca
     4861 tcatatcgca ctctttcaat gcacgatcag caagttcagc cattttcgct gtgtccaagc
     4921 gtttcattaa actacgcgtt ttaagaatag atgtcgcact cattgagaac tcatctagac
     4981 ccatacctac tagcaatgga acagctgttt gatctccagc catttctcca cacatacctg
     5041 cccattttcc ttcagcgtgt gctgcatcaa ttacattttt gatcaaacgt aaaattgatg
     5101 ggttatatgg ttggtacaag taagaaacgc gttcgttcat acggtctgct gccattgtgt
     5161 attgaatcaa atcgttcgta cctacagaga agaaatctac ttcttttgca aacttgtctg
     5221 ctaagactgc tgctgctgga atctcgatca tgatgccgac ttggatcgta tcagatactt
     5281 ccttgccttc acttatcaat ttttgttttt cttcttcaaa gatcgctttt gctgcgcgga
     5341 attctttgag tgtagctacc atagggaaca tgatacgtaa attaccatga acagatgcac
     5401 gaagcaatgc acgcatttgt gtacggaaca tttcgtcgcc ttgttcagat aaactgatac
     5461 gcaatgcacg atatcctaag aatggattca tttcatgagg taattgcaga tatggtagtt
     5521 ctttatctcc accgatgtcc attgtacgaa caactacagg ttttccttcc ataccttcta
     5581 atacagcttt gtaagcttca tattggtcat cttctgttgg gaagtctgga gaatccatat
     5641 acaggaattc agtacggtac agaccaactg cttctccacc attattatgg acaccaacta
     5701 gatcttttgg tgttccgatg tttgcagcta attcaaaatg cttgccgtca gctgtgacag
     5761 tttctgcatt ttttaatttt tcccattctg ctttttgtgc tgcgtaatct gcacctgctt
     5821 tttcaaattc agctttttgt tcatctgtag gatcgatgat tacatcgcct tcgatcccgt
     5881 tgacagccaa aatatcgcct tctttgactt tagcagtaat ttcttttgtc ccaacgattg
     5941 ctggaatttc taaagaacgt gccatgatag cagaatgtga agtacgtcca ccaatgtctg
     6001 ttacaaacgc ttttacaaaa ttgcgatcta attgtgctgt atcacttgga gtcaagtcat
     6061 gtgcaacaac gaccacttct tcgttgatca ttgatggatt tggtaaagta acgcctaata
     6121 agtgcgctaa aatacgttta gcaacgtcac gaatatctgc agcacgctct tgcatataag
     6181 cattatcttc cattgcttcg aacataccga tatacatatc cgttacttct tttagcgctg
     6241 attcagcatt tacgctgttg tctttaatat tttgcttgat ttgacctacc atttctggat
     6301 ctgaaagaac catcaaatgg gcatcaaata cttgcgcttc tgcttcacct aagctttgcg
     6361 ctgctttttc acgaatttgc tgcaattctt cagtagattt agctaatgca gcatccaaac
     6421 gagcttcttc agccgcagta tcttctactg ttgccttact gaatgttaaa tccggttgaa
     6481 ctagcaggta agcttttgca acggctacac cgtcactagc ggcaatccct tttagcattt
     6541 caaccattat tcagataagc cttctttctt cattgtttca acgattgctg ccattgcatc
     6601 agcttcgtca gcaccttctg cagtaatagt aacatcagaa ccttgaccta cacctaaaga
     6661 cataacgccc atgattgatt tcaagtttac tgatttacct ttatactcta agtttacatc
     6721 agagttaaat ttgcttgctg tttgtactaa tagtgtagct ggacgagcgt ggatcccagt
     6781 ttctgctact acgtgaaatt ctttcttttc catattaatc ggtctccttt aagtgagtgt
     6841 tgtttttatg tgagggtttt cttttttagt aattcagaga atcctaacaa atgccaccct
     6901 ttcatctgta aagattaaca ttttttttcg ttagatacaa cagttttcat tcaatttcag
     6961 ataaaagtat tcaaattatt ccggattctt tttcatcctt ttcttcacat tttataacaa
     7021 acgtgtttat ttcctttttc ttgtcgcttt ctcttctcct gacgtttatc attctttcct
     7081 ttgaccctct ttgttttcat atgaaacgaa ttacttgctt ccctttttat ttttagtata
     7141 ataagagtca aaggtcagga atggtcaata gagatttcac cttttctacc tcataaatct
     7201 cttaaaaaac taggggttat tcttttacct catgaaaaac gagatgtaaa atatggatgt
     7261 acccattttt gtgaaaggac gtgaatgatt catgctttgt caaaactgcg gcaagaatga
     7321 ggcaacgatt cacttgtatg ccactgtcaa tggtcagcgg acacaactcg attactgtca
     7381 gagctgttat caaaaaatca aaaatcaaca aaatggaggt ctgactaata tggcccaaaa
     7441 tgatccattt ggatttggca gtttggacga tcttttccgt tccatgtctc gccaaatgca
     7501 acagcaagga aattatgagc aaacaccacc aacacaattt ggcggaaaca acaacttcaa
     7561 tggtggtcag ccacctcaag gcaatacaga aggtttattg ggagaatacg gaatcaatat
     7621 cacagaacaa gcacgacaag gtgatatcga tcctgtgatc ggacgcgatg acgaaatcaa
     7681 acgagtcatt gaaattttaa atcgccgaac caagaacaat cctgtcttga ttggagaacc
     7741 aggtgttggt aaaacagcag ttgttgaagg tttagcacaa aaaatcgtag atggtgatgt
     7801 accgcaaaaa ttaatggaca aagaagtcat tcgtttagat gtcgtatctt tagtccaagg
     7861 tactggtata cgaggacaat ttgaagaacg tatgcaaaaa ttgattgaag agatccgtca
     7921 agcagaaaat gtgatcctat tcatagatga agttcacgaa atcgtcggtg ctggttctgc
     7981 tggtgatgga aatatggatg ctggtaatat tttaaaacca gctttagctc gaggagaatt
     8041 gcaaatggtt ggtgctacta ccttaaatga gtaccgcatc atcgaaaaag acgctgcttt
     8101 agaacgtcgt atgcaacctg tacgggtaga tgaaccaact gtcgaagaaa cgatcagtat
     8161 tttgaaaggt ttgcaaaaac gttatgaaga ctatcatcat gtaaaatata ctgatgaggc
     8221 catcgaagca gccgcaactt tatccaatcg ttacgtccaa gatcgcttct tgccggataa
     8281 agccatcgat ttgctagatg aaacaggttc taagaaaaac ttgacgatcc aaatcgttga
     8341 tcctaaaaca atcgagaaaa aactgcaaga agcagaagaa caaaaagttt tagcttctcg
     8401 agaagaagat ttcgaaaaag cagcttatta tcgcgatcag atcaataaac ttcaaaaaat
     8461 gaaagaacgt cagctcactg aagaggaaac acctgtgatt accgaaaaag atatggaaaa
     8521 aatcgttgaa caacggacag gtattccagt gggtgaatta aaagaaaaag aacagacaca
     8581 actgaaaaac ttagcagatg acttgaaagc tcatgtcatc ggacaagata acgctgtaga
     8641 tcgagtagcg aaagcgattc gccgaaaccg tgttggatta aacaaacaga atcgtccaat
     8701 cggctccttc ttatttgttg gaccaactgg tgtaggtaaa acagagcttg cgaaacaact
     8761 tgcctacgaa ctattcggtt cacaagattc aatgattcgt ttcgatatgt ctgaatatat
     8821 ggaaaaacat agtgtctcta aattgattgg ttctcctcca ggttatgtcg gatacgaaga
     8881 agctggtcaa ttaacagaaa aagtacggag aaatccttac agtcttgtct tgttagatga
     8941 agtagaaaaa gctcatccag atgttttgca tatgttcttg caaatcttag atgatggtcg
     9001 attgacagat gcacaaggaa gaacggtcag ctttaaagat acgatcatca ttatgacaag
     9061 taatgccgga actggtaaag tagaagcaaa cgttggtttt ggtgcggcac gcgaaggtgt
     9121 tactcgttct gtattgaacc aattaaacaa ctactttaca ccagaatttc tgaaccgttt
     9181 tgacgggatc atcgaattca gtgcattatc aaaagaaaac ttgatgacaa tcgtcacact
     9241 aatgctagac gatgtcaacc aaatgctggc agcacagcaa cttcacatcg aagttcctac
     9301 gaatgtcaaa gaaaaattag ttgacttagg ctatgatcca tctatgggtg ctcgtcctct
     9361 tcgacgcacg atccaagaac aaatcgaaga tggtatcgct gaattttatc ttgatcaccc
     9421 ttctatccat gagttgaaag caaaactgga taaagatgga aaaatcatcg ttacttctaa
     9481 accagaacgt ttggctaaag aatcagccga agaaacggct gaataaatcc agttttatag
     9541 cgtgagacgg tatttctttg agaaatgccg tctcattctt ttttcagaaa agtcaaaata
     9601 ctgttcattt tataaaaaag aaattataat aagagaaaca agcaattaaa aatacataaa
     9661 agaggtgagc gccatgaaat tggtaaatgt aacaaacagc cattcccgtc ttgtattaaa
     9721 ccagcttgaa aacactgatg cccatctagt caaggtctac acagccggaa ataccactat
     9781 tgtctacact gaagcccctg aacataatga aattctgctg atcaatgaca aacgtaaaat
     9841 ccagccgaaa gaaatcgaag atgctaaaaa ttatttctta gataaaatca aagacgccgt
     9901 ttaccacgag gatgaaatca aagtaatcga attggcaggc cttgttgaaa tctcgattcc
     9961 aaaacatgaa ttaggcgcta cattagccgt gtaaacaaat tttgaacata ataattaaag
    10021 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcga
    10081 aagcagaagc aactccttcg gaaataagct gaaattcacg aaaattgctt ttcaaatttt
    10141 tgtgaattcc agcttatttc ttggagttaa acgcttctgt ctcagcctct ttttctttct
    10201 agctttttga tttttctctt ttcttttgta cttttctctt ttcattttca gttctacgcg
    10261 tgtctttatt tttaactttt tcctctttat ggatgattga tcgtactttt tgtgcaattt
    10321 gctcgttttt gtcccagtct tttacatcta ataatcgaca taatcgtaac atctcttctt
    10381 ttttctgttt ttttctttca cgtctttgcc attctgtttc catgtgtcac actctccttt
    10441 ctttaatcat atgtacgcaa aataattttt atattttttt gattagacat ttctatgatt
    10501 ttaatatagt aaaaagagcc gtcacggaaa atccgtcacg gctctaaaca aaatatgaga
    10561 ggctgttttt gtttggctat tcttattact tgatgcaggg cagcttgtgt caagacttct
    10621 ttacatcaaa ctttttaatt ttacattggg atatttatcc gcaaaccagc gttctgcaaa
    10681 ttggttctcg aataagaaca acggttggtc gaaacggtca cgagccaaga tattgcgact
    10741 tgaactcatc ttctcatcca aatcttctgg atcgatccag cgagcgattt tgtgtcccat
    10801 tggagacatg attacttcgg cgttatactc atttaacata cgatattgga atacttcgaa
    10861 ttgtagctga ccaacagctc caatgatata ttcttccgtt agatatgttt tataaagctg
    10921 aatcgctcct tcttgaacta attgatagat tcctttgtgg aaggattttt gtttcatgac
    10981 attttttgca gtcactttca taaacagctc tggagtaaat gaagggagtt cttcataagc
    11041 aacttttagt tttccttcga acaatgtatc cccaatctga taattgcctg tatcatatac
    11101 accgatgata tcgcctgcta ctgctttttc gacgttttct ctcgaatctg ccataaattg
    11161 tgtgacattg cttaatttca gttttttatt tgtacgttcc aaccagacat ccattcctcg
    11221 ttcgaatgtt ccagaacaga ttcggacaaa agcgattcgg tcacgatggg ctggattcat
    11281 atttgcttgg atcttgaata cgaaacctga aaattcttcc tcataaggac ttaccatctg
    11341 atcatcttgt gttttgtgtc cataaggagc tggtgccaat tcaacgaaag tctctaaaaa
    11401 agtctgtaca ccgaagttag tcaatgcaga accaaaaaat actggtgtct gatcgccacg
    11461 agcaattttt tcttgattaa atgcatctcc agcctctttg attagttcta cttcttctaa
    11521 gacttgttca tataaggaat tttcagttag aggatgattt tcgggaagca gtccatcttc
    11581 atccaattct actaatcgtt catcctgata attttccgga cgataaaact ctacacgatt
    11641 atggtagata tcataaagac cttctagccc tttacccata ccgattggcc aattcatcgg
    11701 ataagattcg atatctaata gctcttctaa ttcttctaac aattccaatg gttcacgacc
    11761 atcacgatct agtttgttga taaaagtaaa tatcgggatg ccacgttttt ttacaacttg
    11821 gaataatttc tttgtctgtg cttcgatccc ttttgcacta tcgattacca tgaccgcact
    11881 atccacggcc atcaaagtac gataagtatc ttcagaaaaa tcttcatgtc ctggtgtatc
    11941 caagatattt actcgtttcc cttgataatc aaactgcata acagaactag taacagaaat
    12001 cccacgttgt ttctcgattt ccatccagtc tgatttggca aaattacctg tttttttgcc
    12061 ttttacggtc ccggcttgac ggatagcccc accaaacaat agtaattgtt ctgtaatcgt
    12121 tgttttccca gcatccggat gggaaatgat cgcaaaagtt ctgcgattgt ccacttgttg
    12181 ctttaatttt ggattattca taacctaact ccatttcttc cattcaatta tttatatttt
    12241 ctctaggcac agattgtccg atctcttaaa gaaagacaat ttcatatagg gctgtgacaa
    12301 aagccgtgta gcagtctctt tttccgagta aacggcgttc tagagacagc ctatcagcaa
    12361 taagcccacc caagccaaaa atacgaaaag ctgtttttgc ttggctgctc ttattactta
    12421 atgcaggata gctttttcac aacctttgtc aactactaca actgaatcag tatagcatgg
    12481 attcagcgaa tgaaaaagcc accaaaccat aagagtcaaa tatttttttg tcagctgact
    12541 aaaagaaaag acgtgttgct tgtttattca agcaacacgt ctttcataac caagtttcca
    12601 attatcttct tggctcgtca tcttcgaatt cttttctgaa gtatcttcgt tcttcttcag
    12661 gttccgcttc tgtttcttcc ggatcatgaa aaatgacacg gattttaagg attcgagaac
    12721 cttccatttc ttcagaaacc agcgtaatat tatcgacatc aaacgataac ttttcgcctt
    12781 catccggaat catgcctaat gctgtaatca aataaccagc catcgtatct acatcactca
    12841 tatgcagatc actgtcaaaa gcttcattga actcatcaat cagcatacgt ccttggatga
    12901 tatattcgtg ttcatcgatt ttttcataaa gattctcaac ttcatctgat tcatcatcga
    12961 tttctccgac gatttcttct aaaaggtctt ctagtgttgc taacccaacg acgccgccat
    13021 attcatctaa taaaatagcc atctgattct gcgtcttttt catttcatat aacagatcat
    13081 caataaagac cgtttcagga acgaataaag gctcctgcat gattttcttg atatctaaat
    13141 tatcaaagcc gaatttatgg gcagctttta gtaaattctt tgtatgaagg attccgacta
    13201 ctttatcttt gtcttcgtta taaacaggga ttcttgaata gttttcggat aaaacttcat
    13261 tcacattttc ttccacagtg tcgttgatat caatcataaa tgcatccgtt cgtgggacca
    13321 tgacttctcg ggcaactttc gtatcaagag aaaaaacccc ttggagcatt tctaattctt
    13381 cattattcaa tacgccttcg ttttcaagca tataacgcat ttcatctctg gtcatttttg
    13441 aatcttcatc atcaaaagtc atcggcgtga tacgcgccaa taaacttgtg gaagcagaaa
    13501 gcagccagac gaagggctta gctactacac caatcaagcg tatcagccct gaagtgaact
    13561 ttgcgacttc ttctgattta tttaaagcaa tacgtttagg gtacaattct ccaaaaacaa
    13621 tcgatacata ggtcaaaata gccagcacaa tgatatttgc aatatttttt gccgctgtgc
    13681 cgccacctaa taccggagct aaccgttccg ataaagtatt agctagtgaa gcaccagaca
    13741 aaatgttgac caaagtgatt cctacttgga ttgtcgatag gaaattattt ggattctgca
    13801 atatttttaa aagcttttga gatttcacat ctccttcatc agctttttgc tcgattcgat
    13861 tcttattgat cgaaacaaca gcaatttcgg aagctgctaa aaaagcattg atcaatgtca
    13921 aaacgatcaa taataaaatc tgcgctaaca gcgactgact ctcagggtca gcattcataa
    13981 tcgcattctc tccttataaa attaattttt agtttacgat agaaaaaaac gtggcactat
    14041 agccacgtta aaatggtatc acaaagcaat tttctttgca ataaaaacaa gtgtactaaa
    14101 actacctacg aggtcgtagc aattttcttc ttgttttcat tttgcttgtc ttctttgacg
    14161 attttgatta tataggaaat gatcgtcttg cagattgcgt aaaaaggaac acctaagaag
    14221 ataccaagca atccggcaat atttccagct actagaagaa cgataatgat cgttaatgga
    14281 tggattttca gtgatttccc aattacatta ggataaatga tattcccatc caactgttgg
    14341 acgattagca caaccacaca acaaagtaat gcttttaccg gatcattgaa gactgttact
    14401 agaaatgcag gtgctagtcc tagataaggt cctaaatatg gaattaggtt tgtcaatccc
    14461 gcaatgaccc caaataagaa agcataatcc acaccaatta ccatatatcc taaaaatgtg
    14521 aaggtaccaa cgaataaaca ttcgattgct tgtccgctaa tgtaattagc caatgtttta
    14581 ttcagttgcc ccaataactc cacaatttgg tttcttcttt tttctgggaa aaactgttgg
    14641 atgtttggca ctagtttttc tccgtctttc aacatataga acaagatgaa tggtgctgta
    14701 atgattacta cggttgccga agcgatcgtt ccaacgatcg aacctaaact attggatact
    14761 ccactaagga attgctgaat gattgtcccg taagaaatgt ctaattgctc aaaatattta
    14821 ttgatatcta cttccttgaa caaaggcaat tcggctacct cttttaacca gctttctacc
    14881 tgtttgatga aatcaggcat attagatgcc aatgaggaaa tttgctgtac tagacttggg
    14941 atgacactaa gtaaaatcca aataattgct gcaactaaaa ggagcagcac aattgcgaca
    15001 gcccatattc ttttgatttt tgctttcatc aacagcttga caataggatt caataaatag
    15061 taaagaaagc ctgcaatcag tacaggtgca aataacgtgg aaaaaaatgt cccgatcggt
    15121 tcgaaaataa agtttatttc tgatgaaatc atgatcaatg cagctatgat caataattca
    15181 agcgaccaaa acattagttt agactgtcta agtttttcaa acaatagatt tcctcctttc
    15241 tttcttacta gtatatatta tattcgttaa aaattctttt attctttttt tatttttgaa
    15301 aacttgccat actttttatt tgttttattt tccagtatac tatacgaaag gaaggtggct
    15361 agaccatgaa agttgtacaa acaaaagata caatgagcaa tatttactta gatgctgtac
    15421 ggatcaggca tcaagttttt gtagtcgaac agggtgtgcc tttgtctcga gaaatagaca
    15481 aagatgaagc ccattgtatc cacttcgtcc tgtactcaga taaaaaagaa ccgcaaggaa
    15541 ctgtacgtct tttgccttta gaaaacggaa aaatgaaatt acagcggatg gcgattcttt
    15601 ctgaatacag acaccaaggg ttaggaaaaa tattgattga agaagcagaa aactttgcta
    15661 agaatcaagg ctataatacg attttattag gggcacagtc cacagctgaa actttttacg
    15721 aaaaactagg atatacagct tacggcgatc cttttgaaga tgctggcatg cctcatattt
    15781 atatgaaaaa gactttataa aaatttattt cgaaaaaagc aaaaaacagc aagcttttga
    15841 gaaaaatctc tcactggctt actgttcttt tttgtgttta cttacgcgac aattgcttgt
    15901 aacggttata ccaaatatca atatagcctt ccgagaacgg gccatctcca ttattgatcc
    15961 agtcaaccag aatcttcaca ttttctttta ggatgaagtc aatatcgttc gggtaattca
    16021 tcattttgct gtgcatctca tattcatcga catccaatag tcgtttctcg ccgtcaggaa
    16081 ataccttgat atccaagtca tagtcgatat atttcaaggc ctcatcgtcc agcaaaaaag
    16141 gtgaagctag attacaataa taggaaaccc ccttttctct gatcattgct attacattga
    16201 accagtattt tttatgaaaa taaacaatcg ctggttcgcg cgttacccat cggcgtccat
    16261 cagattcagt gactaatgtg tgatcattca cgccgatcat agagtgttcg cttgttttta
    16321 ataccatggt atctcgccac gttcgatgca atttgccgtc gtgcttgtaa ctttggatcg
    16381 tcacaaactc tccttctttt ggaactccca tagcaaccct tccttaagca gcattttgtg
    16441 ctttgtgttt gggtgttcat tacaccgaca tcataattat tatatcaata aaaatgagct
    16501 ttgcctagaa aggattccta ttgtttcgat ttttctctaa ctgatctacc agtttcattt
    16561 ggaccttagg aaaaacgacc gaatcaaatg catcaggctt caaccacttc gaagttttgt
    16621 tctcggtaaa ttcttgttct gctccctcgg ttgcccgtcc gtaaaaaagg agaacatgcc
    16681 atttcagatg actaaaaata tgagtaactt ctcctaaatg tcgtgtctgc caaacaaaga
    16741 gttcctgctt ttcaaatggg agattttgct cttcttctgc tactaggtca tcaaacagat
    16801 cgatttctgg ctttgtttcc cattcttttt tcaatcgctc atactcctct tgtgtcactt
    16861 ccatcattgg aaatgtccac atattcgcca gcaatttttg gctatctctt tcttcaaaat
    16921 aatacgctcc agaatgattt tgaagagctg cactgatata ataaacgtct tttggctttg
    16981 ctttttttgt ttttacagga aaactcgttt ggattcctcg tttgttagct aaacaaaagg
    17041 cttggatcgg acaagtctca cactttggag aagttggtgt acagatagct gaacctagat
    17101 ccatcattgc ttggttgaat tcacctggat gcttttcatc aatgattttc cgcatcgctt
    17161 catcaaagat tttcctgcta gaagcttttg cgatgtctgc ttcaatgcaa aacaatctgc
    17221 tgactactcg catcacgttg ccgtcaactg caggttctgg aagtccaaat gcgatacttg
    17281 caatcgcgcc tgtcgtatat ggtcctattc ccttcaatga gctaatttct tcaggcgttt
    17341 gcggcatttc cccatcaaat tctgacatga tctgtttagc agcagcttgg atgttccggg
    17401 ctcttgagta ataaccaagg ccctcccatg cttttaaaag cttttcttct ggagcatttg
    17461 ctagctcttc aattgtagga aaccattcca tgaaacgata aaaataatcg atcactgtgt
    17521 ctactctggt ttgctgaagc atgatctcag agatccagat acgataagga tcacgattat
    17581 agcgccaagg caggtttctt ttttcttgtt cataccattg aatgaactga tcttgaaatt
    17641 cttttgtttc tttatctgtc catttttccc attctttatt cactttcctc ttccttttcc
    17701 gtaatgaatc gctggatcat atccaaatca aatccttttt gatataagct ttgctttact
    17761 tttagatttc tttttttagg ttcaaacctt aaatttcttc gccatagttt atctccttgt
    17821 ttgactaaat aatcatattc tatttcttga tctgcttctt gcggaagatc agctatcgct
    17881 tgttggatca cttcttgaga aaagcctttc gtcatcaatg tttgttggat ttttctcaat
    17941 aattcttttt ggctttttcc gtgatttttt ccaaacgctt tttcggctgt ctgatgtgca
    18001 ttttgaactt ggtcttcaaa tgtatattgg ctgagcgctt gttcaatgat ttcaggtttc
    18061 aaaccttttt gctgcatctg ctgcgctaat ttctttggtc ctttgtcact taagcgcata
    18121 ttcgtgcgca cataactttc cccatagacc aaatcatcga caaggtccaa ttcttttaag
    18181 cgggctacga ttttatttcg atcttcttga aggatttcct tttcttttaa gtatgttcgg
    18241 atttcttttt ccgatcgcaa ctgataactt aagtaattca ttgctaggtg aaaaccgaaa
    18301 tcagctccgc tactcttctt gatctcttgg atagtttctt cgtctaattc ccgtcctttt
    18361 aataatcgat agcgtactaa tacatcttca gatacttgta aagcttctcc ttcagataaa
    18421 tctacgcgat agaaagggcc tttgccttta ctgattctcg taacggttaa cataaacatc
    18481 ctcctcgaac atatattctc tatcatacca cagaaaatcc ccgtaatcag gtattatatg
    18541 ttagaataaa ccacagaaaa aagaaaaagg gagatactta tggcagaaac aattgtcaaa
    18601 actggacaaa cggttccttt gaaaatcaag cgtttaggca taaatggtga aggaatcggt
    18661 tatttcaaac ggttgatcat atttgttcca tatgcactac caaaagaaga agtactagtg
    18721 aaaatcacga aagcaactcc tcgctatgca gaaggagaat taataaaagt caaaaaaaca
    18781 agcaaagatc gcgtagtggc accatgtcct gtttactatg aatgcggtgg ctgtcaatta
    18841 caacacttag cttatcaagc tcaattggat tttaaaaagg atctgctttt gcaagcattg
    18901 gaaaaattca aaccagcagg ctttcgatct tatcaacttt tgccgacgat cgggatggat
    18961 gagccttggc attatagaaa caaagcacaa tttcagttac gaaaaaataa gcagactcaa
    19021 aaaattgaag ctggtttata cgaagcaaac tctcatcagt tagtccctat aacagactgc
    19081 ttggttcaag aacctcgaac acaaaaagta atgaatactg ttgttcaact attgaataaa
    19141 tttgatgcac ccatctatga tgaacggaca aacagtggaa tcttccgcac aatcatggtt
    19201 agaatcggtg ttaaaactgg ggagttacaa gttgttttta ttactcgcag taaaaaattc
    19261 ccgcaaaaaa acgcaatgat ccgtgagatc aatgaacgac tgcccgaagt tgtttcaatc
    19321 atgcaaaatg tccaggcaca aaaaacgtct ctcgtgatgg gggatgaaac gatccactta
    19381 tggggaaaag aagccattga agaacaaatc aatgaagtga ctttcgatct ttctcctcgt
    19441 gccttctttc aactcaatcc tcagcagacc gaattgcttt atggcgaagg aatcaaagca
    19501 ttagatgtcc agcctaatga aacgatcgtg gatgcttatt gcggagttgg gacgatcggc
    19561 ttaagtgtag caaaacaagc caaagaagta cggggaatgg ataccgttcc tcaagcaatc
    19621 gctgatgcaa aaaaaaatgc ggaaagatta ggatatcaca acactcgata tgaagtcggg
    19681 acagcagagc aagttctgcc taaatggttg aatgaaggtt ttcagcctga tggtatcatc
    19741 gtagatccac cacgtacggg tttggataac gcattgatcc aatcattatt gaaataccct
    19801 ccaaaaaaac ttgtttatat ctcctgtaat gtttctacac ttgccagaga tctggttaat
    19861 ttaagtaaaa agttcaagat tgaatattta cagtccgtcg acatgtttcc tcagactgca
    19921 cgttgtgaag ttgtcgtgaa gatgacgaga ctcgctgact aaatgaaaga ggttgtgaaa
    19981 aagtctgccc tgtatcaagt aataagaaca gccaaacaaa aatagctctt catgttttat
    20041 gaatactgta cttgtgaccg aagagttaac aggaggacac tatttattta aaaaaagggt
    20101 tgtgacatag tttttgtcac agcccttttt gattattatt ggatattcac gtctatcact
    20161 tgataaaatg catttgtcgt atcgctgata ttccatactg ctaaaatcac attatatcct
    20221 tttcgatcct gcggaagtgt gatcgtttgg ttgactgttt ttggtggaac agttgctttg
    20281 tcgtctattt ttgtgatcaa ttcaaaatca ctaaatttca aaggttgatt aggattccaa
    20341 gttggtttgg taatgtagta atcccaagta cttgtccgat gttgtgctgt taaattccaa
    20401 gtaatattta atgttcctgg attaaccatg tttttatgcc atctgttaga tgtctgttca
    20461 tctaaaggct caaagccgct gactcctgca ctagcaattt ttccatcaat gaatgtattt
    20521 ttggtagctt cgatactttg cggttcccat tgtgctcttc cgacattttg atttaagttt
    20581 cctgctgctg atgttcctag gtatgcacgg cttcctggag aagtaatata accatgcgca
    20641 aaacctggaa tgctaaaacc gaacaaccct aaactaacta acccaataaa caataacgta
    20701 ctaatctttt tcatgtagaa acctccttca tttgataacc acattgtaaa cgctttattg
    20761 tattaacaca aacattatgc ttttataatt ttatgttgaa gtaaaaaagg tgaaaaaagt
    20821 aagatgcaga ttataacaga agtagacgag aaaagtacaa atagaaaaca tgagaatcaa
    20881 atcatatgat ctttttcaaa aacatcattc atataaacaa atgttcaact ttttaataac
    20941 aacaattatc aatttttttc ttacttcaca aactaaatac aggtcttgct gtgagccttc
    21001 atggtaaact aatattatag aaaggagtta gtaaaatgaa gatgataaaa gatattcgtt
    21061 tttgggtttg tgttatagga attgtcatat taggcttttt atcagggttg cttagcggaa
    21121 atcccggaaa atattactat tctttgcaac ttccaccatt tgcaccacct agctggatat
    21181 ttgggccgat gtggacctta ctgtatatat tgatgggtat ttctctttat ttgttactta
    21241 atcacaacaa caaaaaacag cgaaacaatc ttgtcggtct ttttgtgatt cagttcattt
    21301 ttaattttat atggtctgct ctatttttta atttaagaaa tattttcatt gcagcaatcg
    21361 atattacact tttagttatt ttcttgtcag tgcttatgta tcagttgtgg ctgcatcatc
    21421 gcctggcaat gtggctcatg attccttatt atttatgggt actattcgca actctattga
    21481 attattcaat ttatttttta aactaagcgc taaaaagaga cgtcctgtaa tttaacacat
    21541 agaaaaagtg ccacctaagt tagatttttt gtctaattta ggtggcactc tttattaacc
    21601 aaactatttt tttctagtac ccacttgttg ttccatactc attattctgg tcataaccat
    21661 aaccattttg ttcctgtcca gcgtaaggat cataactttg agtatcttga cttggtgtat
    21721 agtactgact tgtatcttct gtttgctgat aataggtatt cggatcttgg tactgatcat
    21781 atgtgttttc tgaataggaa gaataatcgg tgtacccttc actattcgat ccatcaccgt
    21841 ttgtcgaatc gccatcttcc gaatcatcaa agaacatcat tgaagcattc tcatttttca
    21901 aattaggtaa tgtatcgctt gtttttatat ttaattgttt cttcagttca ttttggatcg
    21961 aaagaagatc attttttcct aaaatttgat agtagatatc attgatcatt tgtccttcgc
    22021 cttgcaactg gtcttgtttg attgtttcaa acgccggggt gtagttcgtc gctacatcaa
    22081 gcatgtcatc ccaatctaag tccgtcgtca cattgttctc aactgctttt aaaatttttc
    22141 tataattgct tacgccatct aaacttaaaa ctttattgac gattttcgtc actacttccc
    22201 gctgacgacg ttgtcgaccg atatcgcctt ctggatcttc gtgtcgcatt cgagaatagg
    22261 ctaaaccttt ttctccattc aatgtttgtt cacctactgg tacatggaca ccatccaaag
    22321 taaagtcaat tttattattc actttgatgc caccgaccgc attgatcaaa tcttccatgc
    22381 catccaaatt gatcgttaca taatgatcaa taggaatatc gagcagttgc tcaattgaat
    22441 ccatggccat ttcgacacca ccaaatgcgt aggcatgatt taatttatct accgttccat
    22501 aacctacgat attcgtataa atatctcggt ccaaactgat gatcgttgat tttttttgtt
    22561 tcggattaac tgttactacc atcatcgtat cagaacgtcc ttgttccgtt cttcctaatc
    22621 caccggtatc taaacctaat agcaaaaccg aaaaaggttc tttgtcatca atgcttactc
    22681 tctcagaacg tttggaggat attcgattag agtttttaga aattttgttt actgtttggc
    22741 tcgcttctcc cataaatctg attccgtatg ctgataatcc ggcaatcagc aaaacgaata
    22801 cccctaacaa ggttaataga actttttgcc atcgcttcat aatacacctc tgtttcacat
    22861 tttctaaaat attgtctcat ataactgttc aatcagcaaa ttcacttttt aatcataaga
    22921 aaactttaaa aaacattttt gtgtaacgat tattattttt taaaatttct caaaattctt
    22981 aactatcata ccataccgtg aaaaagatcg aaagaccgaa tacacgcagt tcgcaatcgt
    23041 tttatctttt tttatgtatt ttttccagaa aaagcgaaca acaaggtatt ttccaaataa
    23101 tcgtgcagta aaatgatcgc acataccttt cccatctttt taacagtctt tgcctaccgt
    23161 tcagattatc attcgatttc acgacaattt cttcctatga ctggtccttt tgttaaaccc
    23221 atcttctatt cgttttattt ccagctcact gcatggctct taaaatgact cgaactagtg
    23281 attgcattac catcatcttt taatggatag atatgctcct cttcaaagta aaacgatttt
    23341 cttttaaagt aatagaattg acatggtaat gagtatgtat actagtggtt tcacaaatgg
    23401 tcttttattc aacatccaaa aactgaaatt tctttaaaat ccattactaa cgaagaaaat
    23461 gttccttgct tgataattag ttaggacatg tcaagtgaac aaatgcattt acttagccaa
    23521 aaaatctaaa aacttcataa atagacaaaa agacacgact ctctaagaag tcgtgtcttt
    23581 attcaacaaa ttattataat ttagtcactt cagcagcttg aggtccgcgt gcaccttcaa
    23641 caatcgtgaa ttctacttct tgaccttctt ctaggctctt gaagccgtct ccttgaattg
    23701 ctgagaaatg aacaaatacg tcatctccgc cgtctactgt aataaaccca aatccttttt
    23761 cattgttgaa ccatttaacc gttccgtgtt ccataaatgt atatcctcca aaaaagttct
    23821 tgcaagtaac cttgcaagaa cattataccc aatttagaaa acgctagcaa gctttcgctc
    23881 ttttattttc caaattattt ttaagactga tatgacggag gaaatggtta ttttgctgct
    23941 tcgaaacgtt tgtttacttc atcccaatta ataacattcc agaatgctgc aatataatct
    24001 ggtcggacat ttttatattt caaataataa gcatgttccc aaacgtccaa acctagaatt
    24061 ggtgtctttc cttccatcaa tggagaatct tgatttgcag tagaggtaat agctaatttt
    24121 ccattttcca ttacaagcca agcccatcca gaaccaaatc gtccagcggc tgcttttttg
    24181 aattcttctt taaaagaaga aaaatcacca aaagcttcat taatcgcttc ttttatttct
    24241 cctgtaggtt cgccaccagc atttggtgcc atgatttccc agaaaaatga atggttagca
    24301 tgtccgccac cattgttacg tacagctgtc ttgatatctg ttggaatagc gtccatatca
    24361 gataataatt cttctattgt tttttcgcct aattctgggt atttctcaat tgctgaattt
    24421 aaattcgtca cataagtatt gtgatgctta tcatgatgca gatgcatcgt ttcttcgtca
    24481 atataaggtt ccaatgcgtc atatgcataa ggtagatctg gtaatgtgta agtcataaaa
    24541 aaattcctcc tcaatgtttt cttaataatg tctacattac aaagagtaac aaagagaagg
    24601 aaattcttca attgttatgc ctagtcgtct ttttcacttt gacctacttc ttcttcaaaa
    24661 acctcagttt ctttaaaaat aaacaacttg ctgaaaatat agttcgcaag gataacaata
    24721 atttgagtga tgattttcgc cacatactca ttgctgttca taacattgat caacaatacc
    24781 attgccccca tgtccaatcc gtaagaaagg atacggtaga agaagaactt accgcattca
    24841 ataagtgcat gtgtaaaatt cggtgatttt gaatggaata cccaaagttt gttcgttaca
    24901 aaagcaaaca atacagaaca aaaccacgaa atagaattgg ataaggccat acttaaatca
    24961 aaatattggc gtgcgagagc aaaactaaca aaatttacaa tcgtagccaa accaccaaaa
    25021 aagagataga ctagtatctc ccaatatcct tttgcttcta aatatccttt taactgtcga
    25081 tactgcttca tgagccaacc taccttatta aaaattctta tttatcatac tctatatttc
    25141 aataactttc atctttattt aaaaaaatta attttttttg atcctttgcc tttgtctctg
    25201 cttggatcgt cacatgtgta ataccatatt ttaattttaa taaagaaatt atacgaggat
    25261 atatcttttc aatctcgctt agtagctgat cttcaaaatc aatatgtccg tcgaaaataa
    25321 ttatcttttc gctatactgc caaatatgga catgatgaac atcctttacc ccatcgattg
    25381 cttcaatttc ttgttccatt ttttcatagt cgatagcagg tgcactttgt atcaaaatag
    25441 cggcagcttt tttgatcacc attatcgctt ctatcaatat atatatcgaa atgatcagtg
    25501 taataactgg atcgatccag tagatatcga aaaaacggat caaaattgct ccgataatca
    25561 cacttaccga agacagtgta tcacttaaca aatgcaaata actagatttt atattcaaac
    25621 tttcatctgc ttcttgtcgt aataataata ctgagaacaa attcgcgagc aatccaataa
    25681 ctgctactgt tagcatcaaa tgactattta tcttttctgg tgaagtgaac cgcttgaatg
    25741 cttcaacggt taaaaacaag gataatatga tcaatatgaa tgagttgatg aaagcactca
    25801 atatttctaa acgctggtag ccatacgtac gttttttatt tgcttctttt cgggccattt
    25861 tttgtgcccc gtaagaaaaa ataattgcca atgtatcact aaggttatgg aaagcatctg
    25921 aaacaagaga caaactccct gacataatcc ctccaataaa ttctgataaa gtgatcaccg
    25981 cattcaaaac cactactcca aatagttttt ttgatgatct ctgtggctgt gtaggcacta
    26041 ttccttcctc cattttattt atattggata gtctttagac aagaataaca tttttttgat
    26101 atttcaccaa tcattcagct gtttccttaa agcacttgta tttcacttga tacttcgcta
    26161 gaattgcaag tgatgacact actagatgag gagtacttat gacaacaaaa caattaatta
    26221 tcagttcctt tatacgattc aaagacctaa aggaggcgag aaatgtccca ttttggaaag
    26281 ccattgtcta tcttttagtt ttgagtataa ttatggcttt gccaatctct tttcaagttt
    26341 ttcaagtctt aaacaacatt caattggatg ggcaaaagat tgctactcgc attcctgatt
    26401 ttacaatcca aaacgggcag cttcaaaccg aagaaaaaga aggattcatt taccaaacaa
    26461 actccattat cttcacattt gatccagagg gaaaacgaac agaacaggac atctctactg
    26521 atttgatggg gaattttgtt agtgtcggga tgttaaaaaa caaacttatc atagctcttc
    26581 ctaatacagg cacgacttct gctttgttga acaataacca attagaactt ccctacacca
    26641 atgaatcttt aaaaaactta acaggtaagc agctccgttc ttttttaagt gaagcatcta
    26701 taccactttg gataaaagtc ctgacattta tattttctat ttatcctagt tttctgaatc
    26761 tgctgatcac tttgctattt accaacattg ctgcttttct ctatgcacgc tttcgactaa
    26821 caaaagctac ttttcttgat tgtttaaaaa cattgatcta ttcaatttca ttgcctacta
    26881 taattgcaac aatcttgatg gtctttcttc cttcttttga cactagcgcc ttcattgctt
    26941 ttgcaggaat ctttattttt gctcaagctg tcaaaggatg ggcaaaaata tcgatcaatt
    27001 aagaaataaa gacaactcgt tatttctact tcaatagacc cttttagagg ttgtgacatt
    27061 tttttgtcac agcccctttt ttatttgaaa aatatttctt tctcaaaaaa caagcaaggc
    27121 gcttcaaagt tcttcttgta tgcgctacac ttagagtaaa gaaaggcggg aaattaacta
    27181 tgtcagaaag aatcatttat gcagatcgtc catttcgtga tgaatttatc gaagaaatcc
    27241 atactatagc gcctggttat atctttaaaa cagaattaca accagaggat ctacaaaatg
    27301 tagagatcag tttgggctgg aactcagact accaagaaaa actattagcc tcttctacct
    27361 tgaaatgggt ccaatctatc tcagctggtg ttgatcagct accccttgag actttttcaa
    27421 agaaacatat tttactttca aatggtagtg gcatccacag tgaatcgatt acggagcatg
    27481 tcttaggaat catccttggg tattctcgtg gtctttttca agcacaaaag gcacagagtg
    27541 acatgaaatg gttaggttca tcggtccatt accaatctat ccaagggaaa aaaatactaa
    27601 tcattggaac tggccatatt ggaaaaatga ttgcaaaaaa atgcaaagca ttagacatga
    27661 actgctatgg aatcaataca tccggtcatc cagcagaagg aatggaaaag actttttctt
    27721 taaatcaact aaaggaagtt cttcctgaag tagatttcgt agtcaatatc ttacctctaa
    27781 cggaggaaac aactgggctc tttgatcagc atatgtttga acactttgat ccaaaatctg
    27841 tattcatcaa tgttggccgt ggtgcttctg ttaagacaca agatctggtg caggctttaa
    27901 ataaccagca actttccttt gcagcattag acgtttttga ggaagagcct cttccacaag
    27961 atcatcctct ttggaaaatg gacaatgtac tgattacttc ccacattgca ggtttgacac
    28021 ctgattttca aaagaaatta atggctattt ttttaaccaa tctgaaaagc tactttgcca
    28081 cccatgagct tcaaaccaat caagtaaaat tgtctgcagg atattaatag ctgaaaacaa
    28141 tattttcaaa agaaaaaaga gaaggtgaca aaaattgtgt caccctctct ttttttattg
    28201 ttttttaaag tgataggttg gctcctccag gttgggatta taatcaacaa gtaaatcata
    28261 tcctttaaag aaccattcat ctgcttcctc gataaaaaaa agtgtcccat caatttccac
    28321 tttctttaca ggatgttctg gctgttcaac tgacattcca atcgaaaaac catcatgaac
    28381 ctctgttttg ccgtagactt ttccaaaaaa acgtattccg taagtttctg gcagatcaat
    28441 ttctgagtca aaccactttt gtgcttttgg cgttaccgtt aatttcatcc caacaaccta
    28501 ctttctcttt tctttctatt gattcatctt atcgtctaga atatccacat cgcctgtctc
    28561 tccaataacc agtagattat cattttcacg aacatattca tctgctgcag gagaagcgat
    28621 aaccgtttga tctactcttc gtatggcgac tactgttaag tcaaaacgtt gcctgaaatt
    28681 taattcagct aatgttttgt tgaaaaattt aggattggag actcggattt ctgctaaaga
    28741 atatttatct gacaactcta ggtaatctaa aatattcttt gacactaaat tatgcgcaat
    28801 ccttattccc atgtctcgct cgggatggac gacgcgatca gctccgatct tttcaagtac
    28861 tctggcatga tactcatttt gggcttttgc taaaatattt ggtacgccca tttcctttac
    28921 catcagagta actaagatac tggcttgaat atcttctcca atagcgacaa ttacatgatc
    28981 gaaatttctt atgcctaatg atcgaagtgt catttcatcc tgtgcatttg ccacaacagc
    29041 atgagtggct atattcatat attcattgac ccgatcttca ttactatcaa tagccagtac
    29101 ttcttgacca gcttcgatca gtgttcggca gatacttccg ccaaatcgtc caagacctac
    29161 aattgcaaaa ttttgtttca ccgttttcct cctttagtat cttcttttct cagtataaca
    29221 aagcctttac aaaaacaaaa gcaagcatca ttattgtaaa aaagccaaga aaaataatag
    29281 ttattttctc agcttttgat tatcctcttt atcatcgttt ttatttcacc aaaccatgtt
    29341 tgaatgcata gatagcggct tgtgtacggt cttccacttc tagcttagcc aatatattgg
    29401 aaacatgtgt ttttactgtt ttcagtgtaa taaacaattc gtccgctatt tcctgattac
    29461 tttttccttc agaaatcagc attaaaattt cattttcccg attcgttagt tcttcatgta
    29521 atacaggctc atttcgccga ctcatttttt ccatcatttt tgttgtcact tctggttcta
    29581 atactcgctc gccacgttgc gttgccctta tagcatcagc aatttcatgt gcggtagagg
    29641 tcttcaacaa ataccctgct gcacctgctt caatggcagg ataaactttt tcgtcatcaa
    29701 taaaactggt tacgataatg attttagctt ctggccaatc ttttaagatt gcttttgttg
    29761 attcgatacc atccatttct tccatgacta agtccattaa aatgacgtcc ggtcgaagtg
    29821 tcatcgcctt ctcatagcct tgtcgtccat tttcagcttc tccaattact tcaatgtctt
    29881 cttgaataga taaataaaaa gagacgccta agcgcaccat ttcatggtca tctactaata
    29941 aaacttttat catccttatc tatgcctcct tcatcaaagg gaccttgatt tctacacttg
    30001 taccttgacc tttaaaacta atgattttta ctgttccacc aataccagct actcgttctt
    30061 tgatattgtt caatccataa cttcccgttt tcgtttcatt catattaaac cctgttccgt
    30121 cgtcaataat acgtaacaaa agattattgt ctattttgtg caaatatacc tctaattcat
    30181 tggctttagc atgtcttaat gtatttgata acaattcttg aacgattcgg aacaagtgat
    30241 cctcaatgga actagatagt tttacatctt ctacatccca tttcagcgaa atctggattt
    30301 tattttgcaa ttccttcaat aactgctcga taccttgctt taaacttttc tcttctaaat
    30361 tgactggacg tagatgaaga agcagcgcac gcatctcaga ctgggaagcg ttgatgatct
    30421 ctgctaccat tttcaactgc ttgcgaaaca tttctggtgt ctcgcttttt tctgcttgct
    30481 ccgtcaatgc cgacatcatc atcattgctg caaacaattg ttgcgaaact gaatcatgca
    30541 gctcacgggc taatcgatgt ctttctaatt ctaaaatttc ttctttggtt tgtccatcta
    30601 catattgggg acggctcgtt acaatttgta attcactaga tacttcaatc attttttctt
    30661 ttatcttagt gatttcctta tcaatatctt tgatgtataa atcgtccgat gcacttggga
    30721 tcggaagaag caacaatttg gattcataat tgccagaaga aagtgcacgc agcttttctt
    30781 cgattttccc gtattttgtc ttttgaataa gtgataataa tagaaagaca attaatccta
    30841 cacctaagga aatggctaac aaatggaaga taagcggaac atataataaa cgtgcacgca
    30901 gtaattctcc ccaccaatga ctttgattgc tggcataaaa ataagtgaaa agtgaaaata
    30961 gaataataag gaaagccgca atccccgaat aaacagctag catcgctctg gatatttttc
    31021 ccatcatatt cgtatcacct cgacatctcc aatcaaggta ttagtaataa tcttcaatct
    31081 acgagggttt tcatcataat cactgctgta gattttgatt tgttcatttt tcaaagaaaa
    31141 ttgctcttct tcaaacaaga catttcctac taatgtcgca tgctctaatc ggatggctac
    31201 gcccaaaggt actaagatcc gtgttctgcc tatccctttt cttacaatca caatattatc
    31261 atcttttggc aaaagcgtat tgcccaaatc tatgatagta tcaccagaaa tgacggcgat
    31321 gttgatatcg tcccattcat agacgtcgtt tccaatacgt tggttaccga ataattgctg
    31381 tcttttccgc tcattattat gtgtttttaa ttgctctgtt tggatcatca tgatctgttt
    31441 ctttcgccaa aaagcatttt tggttaaatc tatccctgaa atctctacac ctttcagacc
    31501 aataaataaa acggcaaata cagccatcat ccataaagca ggactgttga ctaagctaaa
    31561 aaaaatgacc aaacttccta atatcaattg aaaattttga aatttagtac gaggatgttt
    31621 gcgcatagct agatatatat tgaaaatacc gaatatcact aaaagcaaaa gtccagtatt
    31681 gtttacgatt tgccaaacag caaataacag cagtagtgct tcaactacca caaaaaaacg
    31741 ccaagaacta ttcatcgctc tttctccctc ctgacacatt ctcatttcat catacctatt
    31801 tttttaactg ttggctatag gactttagat gtaagatcgt ttgttgaaat ctaaaataca
    31861 caaagagaaa aagatctaga gtgtatcatc agtaagatga actctctaga tctttcagtc
    31921 agactacgct tgttctactt tgacgatttt aactaacata tcgccacctg gagtagcaat
    31981 agttacttca tcatttttac gtttgccaat caatgcttgc gcaataggag aatcattgga
    32041 aattttgcca gaaaacggat ctgcttcagc gcttccgaca atcgtgtact cttcttcttc
    32101 tccatctggt agttcgatga atgtaactgt tttaccaatt gatacctcat ctgcatctac
    32161 accgtcgtta tcgatgattt gagcaaaacg aatcatattt tcaattgttg tgattcttcc
    32221 ttcaacaaat gcttgttcat ctttcgctga ttcatattca gagttttcgg aaagatctcc
    32281 gaaaccgcgg gcaattttga ttcgttcaat gatttctccg cgtttgactg tttttagttc
    32341 ttctaattct ttttctaatt tttctttacc ttctagagtc attggataaa ctttctctac
    32401 catgtgtaac gtctcctttt ttcgataaaa taccgcaaaa ggtaatcttt tgaggaatat
    32461 agtccaaaaa agattaccac gcttttcttc aaatgtaaat gtttttctaa aaatgatcaa
    32521 tgtctagagc ctgtttttct aagaattatt tacatatttt tccactaatt ccatatgttc
    32581 ttcataagtt ttagcgaaat aaacatttcc agtgctcgta tctgctacga aataataata
    32641 gtcattttct gctggttcta atactgcctt gattgcctct tcgcttggat tgtcaaaagg
    32701 tccaggtcca tagccggtat tggtatacaa attatatggt gaatctacag ctgtatcttc
    32761 atacgtcact aattctttgt gttcacctaa tgcgtataaa acagagatat ctgactgaag
    32821 tggcatattt gctttgattc ggttaaagaa gacttgtgca atgtttttac gatcattttc
    32881 ttttacgcct tctttttcaa ctagagaagc tagagttaaa acttcttgta cagtcaaatt
    32941 tttctgcttg atagctgaat aatagttttg catgaccgta ttcgtcttgt tcaccatttc
    33001 aatcactaga tccttaagcg aagtactcgt gtaataatca tatgtggctg ggaataagta
    33061 accttccaaa cggtattttg tgttctcagc tttagatgca ctttccaaca gttctgggaa
    33121 tttttggtgc aactcgttaa agaaagtctc atcattcact aaatctaaaa attctttctt
    33181 gtctttacca gttaccttcg ctacccgttc agcaatctga tcgatatcgt acccttctgg
    33241 aatggcaatt ttcccatctg caacttttgt tggctcagaa gtaccgcctt cttgcaactg
    33301 tttgccaatc tcgtcaagag tcatattagg cgctaactga taatagccag cttgaaagcc
    33361 agttaaattt ttaaattttg tataataatt gaaaacaatc ccgcttttaa taatattgtc
    33421 cttctccaaa atctcaccaa tctgcttgtt agaagagcca ctcgggatct cgacttggac
    33481 taattggtca tctgatttat ctaaaggctt caacccgcta tctacataac gatacacggt
    33541 aaatccaaga aatccgccaa taatcaataa tgtaagagca atcactaaaa tgatcttcct
    33601 cacaatctta tcttcttttt tacgcagatt tctccgcttg tcgattatat ctgctgtagc
    33661 tgccttactg gcttcttttt tatctttact tgggtgttgt ttgtttcctg acaaaacaac
    33721 tcctccttac tatcaagttc gtaactttat tcattataca tgatatttga attggcgtga
    33781 atgattttat ttaaatattc tatcaaaaat gtaagtagta aatccattac tataagatta
    33841 aaataaatgt tatcaagata caagctattt tagctttatt ctttctgtaa aaaagaaagg
    33901 agactaaata aactattatg aaaaaaggaa aacatgctaa aaaaagaaaa tattcatttt
    33961 taatgatcgt tgtcgtgttg tttagcctta gtgcgttctc tacaggaact attcttggat
    34021 ggcaatttta caataatgaa cactctcaga tagttgccca tgctcaatca gctcaaaaaa
    34081 agttgatact gactaccaat gaacaacagc tcgttgaaaa aatcgatcaa ctattaagaa
    34141 aaaatgatta tattggctca atttacgtaa agaaaaataa tcgcatcatc atggaaaaag
    34201 gctatggcta tgcgaataaa ctcacgcaac aaccaaatga tccttctctt tattatcaga
    34261 ttggttcggt tcaaaaagca ttgactactc ttttgatttt aaaacaagtc gagcgtgggc
    34321 acctttcttt agatactaag ctggctcaat tctatccaca aattgctggc agccagcaaa
    34381 ttactatccg tgacttgctg tatatgcgct caggcttgca ccgaactgct tctccaacca
    34441 taccgatgac tgatgaagaa gtcgttcaat ttgcgattca gcacttaaaa tttacagatt
    34501 atcagacctc tcattatgat tcattgaact ttaccatatt gactggaatt ttgatcaaat
    34561 taacaaatca accgtatgaa aaattattga ccgatgatat catcgaacct ttaggattac
    34621 agcatacaaa attttatgac caagtcaaaa attcatccaa tcatgcacta tcctatcaaa
    34681 tgtctaaaga ggatgattac tttaaaccac tgacagaatc tgaaacagag atccataacg
    34741 aattagggac tggtaatatc agtatgtccg tctatgattt aaatacgctg tttacacaag
    34801 ttttatctgg aaaactagtt tccaaagact tactcttctc attatggcgt gaaaatgcga
    34861 atggacatcc atatagtggt ggggtatata gcggagataa ttatatcctg tcgcaaggaa
    34921 atatcgactc ttttgataca tctgctatat ttagcaaaga ttcacaggat gctatcatca
    34981 tggaaagcaa tataaaagct gacaaaacta tcaaactgcc tactgctgat ctaagaaacc
    35041 aaatctatga cctgatggaa ggaactacta agttggcaaa tagctgatat gatgaaaaga
    35101 tagcaaatac tacttctttg aatagcattt gcttaaaaaa agtactcgac taaacgtagg
    35161 aaagagtgtg ggacaaatct aaaaatcaat ttttgctaca gactttaatt ccaaataaac
    35221 agaacagaaa gaggtgttct tctttctgtt ctgtccaaat tattctaaga tttctcttta
    35281 ccaacattat ttgacaaagg gcctattaaa tctaaaatag tatctttaga ttgctaaaat
    35341 atgtgctagc aaatcattca tggcttgttc ttatttttaa ttttatgcaa taaaaaagaa
    35401 gaacaattag cgtgattata gaa
//
LOCUS       DAZAFF010000017        34714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000017 DAZAFF010000000
VERSION     DAZAFF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.017"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..203
                     /locus_tag="ACG9BB_000857"
     CDS             <1..203
                     /locus_tag="ACG9BB_000857"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836824.1"
                     /translation="LHKFGLIPRNWLLNTVYQEVVSLPFNSESQGAISKNSFSGLLTI
                     VTCDRGVGRRTPFKEEVVKSAV"
     gene            complement(366..2258)
                     /locus_tag="ACG9BB_000858"
     CDS             complement(366..2258)
                     /locus_tag="ACG9BB_000858"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2836825.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2255..3982)
                     /locus_tag="ACG9BB_000859"
     CDS             complement(2255..3982)
                     /locus_tag="ACG9BB_000859"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2836826.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3998..4648)
                     /locus_tag="ACG9BB_000860"
     CDS             complement(3998..4648)
                     /locus_tag="ACG9BB_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFN2836827.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4852..5148
                     /locus_tag="ACG9BB_000861"
     CDS             4852..5148
                     /locus_tag="ACG9BB_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN2836828.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5340..5960
                     /locus_tag="ACG9BB_000862"
     CDS             5340..5960
                     /locus_tag="ACG9BB_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN2836829.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6052..7218)
                     /locus_tag="ACG9BB_000863"
     CDS             complement(6052..7218)
                     /locus_tag="ACG9BB_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN2836830.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7230..7583)
                     /locus_tag="ACG9BB_000864"
     CDS             complement(7230..7583)
                     /locus_tag="ACG9BB_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFN2836831.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7711..8733
                     /locus_tag="ACG9BB_000865"
     CDS             7711..8733
                     /locus_tag="ACG9BB_000865"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFN2836832.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8752..8913
                     /locus_tag="ACG9BB_000866"
     CDS             8752..8913
                     /locus_tag="ACG9BB_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836833.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9172..9606)
                     /locus_tag="ACG9BB_000867"
     CDS             complement(9172..9606)
                     /locus_tag="ACG9BB_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836834.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9716..11575)
                     /locus_tag="ACG9BB_000868"
     CDS             complement(9716..11575)
                     /locus_tag="ACG9BB_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN2836835.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11576..11716
                     /locus_tag="ACG9BB_000869"
     CDS             11576..11716
                     /locus_tag="ACG9BB_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFN2836836.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11693..12853)
                     /locus_tag="ACG9BB_000870"
     CDS             complement(11693..12853)
                     /locus_tag="ACG9BB_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN2836837.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13160..14584)
                     /gene="araA"
                     /locus_tag="ACG9BB_000871"
     CDS             complement(13160..14584)
                     /gene="araA"
                     /locus_tag="ACG9BB_000871"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFN2836838.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14600..15298)
                     /locus_tag="ACG9BB_000872"
     CDS             complement(14600..15298)
                     /locus_tag="ACG9BB_000872"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFN2836839.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15255..16916)
                     /locus_tag="ACG9BB_000873"
     CDS             complement(15255..16916)
                     /locus_tag="ACG9BB_000873"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFN2836840.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17134..18534
                     /locus_tag="ACG9BB_000874"
     CDS             17134..18534
                     /locus_tag="ACG9BB_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFN2836841.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18786..19865)
                     /locus_tag="ACG9BB_000875"
     CDS             complement(18786..19865)
                     /locus_tag="ACG9BB_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2836842.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19951..20910)
                     /locus_tag="ACG9BB_000876"
     CDS             complement(19951..20910)
                     /locus_tag="ACG9BB_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFN2836843.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20922..22433)
                     /locus_tag="ACG9BB_000877"
     CDS             complement(20922..22433)
                     /locus_tag="ACG9BB_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFN2836844.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22455..23018)
                     /locus_tag="ACG9BB_000878"
     CDS             complement(22455..23018)
                     /locus_tag="ACG9BB_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN2836845.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(23024..23887)
                     /locus_tag="ACG9BB_000879"
     CDS             complement(23024..23887)
                     /locus_tag="ACG9BB_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2836846.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23901..24797)
                     /locus_tag="ACG9BB_000880"
     CDS             complement(23901..24797)
                     /locus_tag="ACG9BB_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2836847.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24972..26261)
                     /locus_tag="ACG9BB_000881"
     CDS             complement(24972..26261)
                     /locus_tag="ACG9BB_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2836848.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26233..27750)
                     /locus_tag="ACG9BB_000882"
     CDS             complement(26233..27750)
                     /locus_tag="ACG9BB_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFN2836849.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27948..28838
                     /locus_tag="ACG9BB_000883"
     CDS             27948..28838
                     /locus_tag="ACG9BB_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFN2836850.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28911..29735)
                     /gene="nadE"
                     /locus_tag="ACG9BB_000884"
     CDS             complement(28911..29735)
                     /gene="nadE"
                     /locus_tag="ACG9BB_000884"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFN2836851.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29747..31219)
                     /locus_tag="ACG9BB_000885"
     CDS             complement(29747..31219)
                     /locus_tag="ACG9BB_000885"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFN2836852.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31856..32218)
                     /locus_tag="ACG9BB_000886"
     CDS             complement(31856..32218)
                     /locus_tag="ACG9BB_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFN2836853.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32441..33193
                     /locus_tag="ACG9BB_000887"
     CDS             32441..33193
                     /locus_tag="ACG9BB_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN2836854.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33333..34256)
                     /locus_tag="ACG9BB_000888"
     CDS             complement(33333..34256)
                     /locus_tag="ACG9BB_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2836855.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34271..34543)
                     /locus_tag="ACG9BB_000889"
     CDS             complement(34271..34543)
                     /locus_tag="ACG9BB_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836856.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10245 a         7222 c         5988 g        11259 t
ORIGIN      
        1 ttttgcataa atttggctta ataccgagaa actggctttt gaacaccgtt taccaagagg
       61 ttgtgagcct gccgtttaac tccgagtcac aaggagcaat ttcaaaaaac agcttctcag
      121 gtttgttgac tattgtgact tgtgaccgag gagttggcag gagaacaccg tttaaggaag
      181 aggttgtaaa atcagcggtt tgacctgagt attagagata caaaacggaa aaatagcttc
      241 tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca ccgtttattc
      301 ggaaaaaggg actgtgacac gacttttgtc acagccccta aaaaaaataa taaatttgta
      361 ctattctatg aagcttcttc ttcagaaaac tgactgttat aaagatctgc atagaatccg
      421 tctttttcca tcaatgtatc gtggtttcct gtttctacga tcgaaccatg attcatcaca
      481 ataatgttgt ctgcatcgcg tattgtagac aagcggtgag caacaacgaa acttgtccgg
      541 ttttccaata ggcggttcat cgctttttga atcagtattt ctgttcgtgt atctacacta
      601 gatgttgctt catcaaggat caggacatct ggatttgcta agaatgcccg cgcaattgtg
      661 attagctgac gctgcccttg cgaaatattg cttgcatcct cgtttaatac agtgttatat
      721 ccttctggca atttacgaac aaaatcgtct acgtgagcag ctttagcagc ctcaatgatt
      781 tgttcgtctg ttgcatcgtc gttcccatat ttgatattgt catagatact tcctgtaaac
      841 agccaagtat cttgcagtac cattgaaaac tgtgcgcgca attcatcacg tgaaagatca
      901 cgggtatcta caccatcgta acgaatacta cctccagaaa catcgtagaa ccgttcaaga
      961 aggttgatca gtgtcgtctt ccctgcacca gtcggaccta caatagcgac catctcacca
     1021 ggtttgacat ccaagttaaa atcagtcatt agcaagttat ctggtgtgta gccgaattga
     1081 acattttcaa aacgaacttt gtatggtgta tctttttctt ctggaaggcc agatttttca
     1141 ttggacatct cttcttcatc caacacttca aatacccgtt cggctgaagc aactgttgat
     1201 tggatagtgt tcatcaaact tgcaagttga gtgattggtt gagagaactg tgttgtatat
     1261 tgcaagaacg cttgaacatc ccctaatgtc atgttcccat ttgcaacttt tactccgcct
     1321 agtacagcga cgaacacata gcctagattt ttaacgaaat tcatcaacgg catgatgatt
     1381 gccgaaataa attgagcttt ccaaccagcg gcatacagat tctgattttc tttttcaaat
     1441 acttcctggt ctttttcagt atgattgaat gttttaacaa tggtatgacc accatatgtt
     1501 tcttctactt ggttattcaa taaacctaaa cttttttgct gatctgcgaa aaacacttgt
     1561 gatttcggcg ctacgatcat cacgatgatc aagcttaaag ggatcatcaa taacgcgact
     1621 aacgtcaatt gccagctgat tgaaagcatc atcacgatga ccccaatcaa tgtgactgca
     1681 ctagttacaa attgagtcaa attttgctgc agcgtgcttg cgatgttatc catatcattg
     1741 attgctcgag acatgatatc cccatttgta tgtgtatcat aataggaaat cggtacacga
     1801 ttcattttgg cttccaaatc atgacgtaat tcataaactg ttcgttggga aacacgtgtc
     1861 ataataaatt gctgtaaaaa gttgaataca gcagaaatca aatacatcac gattactgtc
     1921 gcaatgatct gcgcgatttt atcaaagtct atcggaaatt ttgttacttg ttggccttgt
     1981 tgcatctgtt gagcgccagc cattacacct ttgaatattt ctgtggttgc tttccccaag
     2041 acctttggtg tttggatttg gaagatcacg gctgctattg ctagtacaaa tacagcgatg
     2101 atcgcaacca tacgtttaga catatagcca aacaatcggc gtaccgttcc ccagaaattt
     2161 ttaggctttt caccttttac accgagattt ctgccaggtc caccatgtcc tggaccgcct
     2221 gctggtccat gagagggacg tgtgtttgtt ttactcatgc taagtcctcc tctcttagtt
     2281 gtgaatgaac gatttcttga taagtttcgt ttgttgctaa tagttcttca tgtgttcctt
     2341 tgccaaccag ttttccttca tctagcacaa ggatcatatc tgcatccatt actgtactta
     2401 ctcgttgagc aactaaaacg gtgatacttt ctttcatatt cgttttcaat gcttggcgaa
     2461 gattggcatc cgttttgaaa tccaacgctg agaaggaatc atcaaacaca tagatatctg
     2521 ctgttttgac taatgcccgt gcaattgcca gacgctgtct ttgtccacca gagaagtttc
     2581 ctcctccttg ttccacatgg ctatctaagc catcttccat ctctgaaaca aaatctttag
     2641 cttgtgcgat ttctaatgct ttccaaatca tttcatctgt tgcatccggc ttaccatact
     2701 gcatattgtc tcggatcgtt ccagagaaca gaactgcttt ttgaggaaca aagcccatta
     2761 attctcgcaa attatattga gtcatgtcac taatgtccgt tccatttatt tggatcgaac
     2821 cagattcgat gtcatataat cgagggatca aattgactaa ggtcgtttta cctgaaccag
     2881 tcccccctat gattgcgacg atctcgcccg acttggcttg gaaatcaata tcttccaatg
     2941 caagtttttc tgctccatga tatcgataat tgacatgatt gaaggccaat gttgcgtttt
     3001 ttccagcaaa agaaactgtt ttaggatttt cagggtcatg gataccgatt ttttcatcca
     3061 acacttcatt aatacggtca gctgaagctt gtgcacgagg caccatgata aaaatagcgg
     3121 aaagcatcat aaagctgatc aagatctgca tcgcataagt cataaatgca atcagattac
     3181 cgacttccaa agtcatatct gctatgtagt gtccaccaaa ccaagtgatt gctacgtttg
     3241 taccgctcat gatcaatgtc atcattggca tcatcaatgc gacgatcgta ttaactttga
     3301 ttgccgtatt tgtataatct ttattcgcta aatcaaaacg attttcttca aagcgtgtct
     3361 tatcgaatgc tcgaatcact ctgacccctg tcaatccttc tcgaaatacc aggtttaaac
     3421 ggtcagtttt cttttgcata cttttgaata atggaacagc aaagtacatg attccgccga
     3481 tcaaaataat cattactggg atgacaacta agaagatttt tgtcaactga tgatcttttt
     3541 gataagcaag tatgctcgcg ccaatcaacg tgattggtgc attgatcatc atacgtaaaa
     3601 acatttgtgt aaccatttgg atttgagtca catcattggt cgttcttgtg atcaaagagg
     3661 ccgttccata tttatcaaac gcatcttttg tcagactctc tgatttttta tagacatccg
     3721 tacgcaattg cttacccagt ttctgtgatt cacgagtagc aaaaaatgta tttgcgatag
     3781 cagctaagat actgatcaaa gaaaagccga tcatcacaaa acctgtatgc caaatataat
     3841 cgacatctcc ttgcgctacc cctttatcaa taatattgga ggtaagtgtc ggcaaatata
     3901 aatctgccag tacttggatc accatgaaaa ctgcagctgc aattgctgaa gtcaaggaga
     3961 tccgttttac gatttttagc atctagttcc cttctttcta tttatatact ccgtttttca
     4021 gccaagaaag tttcaaattg aattcactga ttgcttgctc tactgagtaa tcttcagatg
     4081 ttgaaaataa gcgatacgaa tgcgcaattg ttgtaaatac agtattcatc actaattgga
     4141 tcagtaattc caattcatga tcatttttta ctttcagctt cgtccgatcg atcaattcaa
     4201 gcaactcttt tgcatcttgt ttgtgttctt tatgtggatc gtgaggcgtt ttttggcgac
     4261 tcgtatgagg agcgactttt cttgaggcac ggtaatccat gttcataaac aagttacgat
     4321 aaaaagaagc attctcgcct tccaaaatct cataaatcat tttagaaaaa taggattcga
     4381 atcctttgaa tagatcacca tcggattctt tcattagttg taacatatct ttagagctgt
     4441 cacgtctcat tgtctcaaag taataaaaat aaagatcctc tttatcttca aaatactgat
     4501 agaaactgcc tctagggata tccgctaatt tgacgatatt agcaatcgaa gcttctttta
     4561 agggtactct agaaaactcg attctcgcag cctcgattag tcttttttgt ttttctttag
     4621 gcaaatggaa aaatgtctgt gtcggcatat ggattctcct ttctttcaag atgacacatt
     4681 gtcatatgac gctatgtcat tataacacct cctacctgtt ttgcaagaca tttgcatagt
     4741 ttttttcatt atcatccgat acattaaaaa agtgataaga ggaatccttc catttgaatt
     4801 aatgagagtt gcccattaaa atagccacat actaaacaaa ggatgagata aatggcccaa
     4861 tcaattagtg tccaagactt caataaattg cctctcgatg ctgacagcat cgttttagat
     4921 acaagaaata ccttagatta cacaaaaaac cacttaagcc actcgctttc tatccccgct
     4981 gctatcttgc caagacaatt aaaaaatcta gatccaaaga aactttatta tattctaagc
     5041 tatacaggca ggcgatcaga agcattagcc gatcaactta ctgctaatgg ttttcgtgct
     5101 gtttatatca ttggtgggat ggaagctctt cagtcgagtg ttgcttgaat attgcttgtt
     5161 agattcaaaa gaggctgtga caaaaaatcg tgtcacagcc tcttttatac cctgtctttt
     5221 tttagttatt tattcttcaa ccttatcaat atgacttgat agccttttct ttttcgagta
     5281 aactgatttt gttagtcata gttataacaa tcatagcaat aaaaggagaa ggtaagcata
     5341 tggcatctgt tttagtagtc aaaggtcatc ccctaactgc agaagaatcg cgcacagtta
     5401 aggcattgac gtctttttta acaagttata aagaaaatca tccagatgat gaagtgactg
     5461 ttttggaact ttatcgcgat gatattccgg aaatcgatga agaattatta tcaggttggg
     5521 aagcattacg tgctggtgca gaattcactt ccttatcaga aagtcaacaa caaaaaattg
     5581 ctcgtttcaa tgaattgact gatcagttcc ttgctgctga taaaatcgtt atcgcaaacg
     5641 ccttatggaa tctaaatatc ccaacaaaat taaaagcttg gttcgatacc gtcaatgtag
     5701 ctggaaaaac gttccgctat acagaaaacg ggccagaagg attagtgaca ggtaaaaaag
     5761 cactgcacat ccaatctaat ggcggtgtat acaacggaca agactttgcc tctcaatatg
     5821 taaaaggtat tttaaacttt gtaggcatcg atcaagtaga tcagctattc atcgaaggaa
     5881 tcgattatga ccctgaccgt gcagatgagt tgatgcaaaa tgcattagat aaagctgctg
     5941 ctttaggtaa atcattttaa aaattgagat aaatgcatac aaaagagaca gaggaaaaaa
     6001 tcttcaaagc agcatttgtg ccttggatat tttcccatct gtctcttttt gttattcttt
     6061 ctgctttttc aacggatttt cttcataaag agaagagatt gttgtgtacc attcaaaaac
     6121 atgagtaatc actactttag ctgctgcata aacaggaatc cccaagatca caccgatgat
     6181 tccgaacaat ttcccagaag tcagcagaac taataaaatc gttactggat ggattgctaa
     6241 ctgattccct agtactaatg gagagatcaa gcgtccttct attgtctgct ctactgcaaa
     6301 aacgatcagt actttgatga tcatgaatgg acctgctaca atggctaaaa agatagccgg
     6361 aatcatcgct aaaaatgatc ctaagtaagg gatcaggttt aaaaaacccg caataatccc
     6421 caacgtgaca gcatactcta aaccaatgac cgcaaaacca atcataaaca tgattgccac
     6481 agcaaaagcc acagttagtt gtcctcttat ataagaagaa acttgactat tcatttcact
     6541 cagcacattc aacgtcggtt tttgcatgcg tgtcggtaaa aatttgacaa aataaggtgc
     6601 taattgttta ccgtctttca atagataaaa taagataaac ggcatcgtca gcagagccac
     6661 taaaatagtc gctaccgcgc cgacaaaact tccaagactc tgaactgtac tcttagatat
     6721 atcttggatc atatcgcctg ccgaattcat gaacttatca cctgctgttt ccaactgttc
     6781 gcgaaattgg ttgaacagcg ggtcccgcaa aatctcggtt aatttattat caatcgtatc
     6841 tacatattga gggaagttac tgatgaagct cactgtttgt tcctgaatct tagggatgat
     6901 cactacgctc ccccatgcga ttagtgcaac aaccaaaaca aacagcccaa tgatactgta
     6961 aagccttggt acttttcgct tttcaaagta atcaactacc ggattcatca agtaatacaa
     7021 gattcctgct aaaatgatag gcagacctac gattgctaaa aattgccaga ttggtgcaaa
     7081 caaatatgaa acttttgtaa ataaaaagac gatcagcaat attaacaata caacaagtaa
     7141 ggctgtgact acttgattat ttaaaaacca gcgccaaaac cacgataccg gtctgttctt
     7201 cttatttttt ccttccataa ttcctcctgt tagttataaa aaatttggct tcccaaacca
     7261 atagcaggca gtctatgcgg atgaagatag attccatttg caatttgtcc ttcttctgga
     7321 caagcatcaa aaatcaatga tacgtgagct actgtcttta aattcccatt ggccatcgga
     7381 ccgacataat cgatcgtata tacctgatca tcaaaagcaa ttgttccgcc tgctttcaaa
     7441 gaaaaaggtt cgtcttttga tacttcttga atcaccgaat attttcgtaa ggcagctgtt
     7501 gctgttttat cgaacaaaat cagcatgggt tcttccttgt ttattgcttc tgtcccaatt
     7561 tctgtaatct ttgcctgaat cattgcactc tctcctttaa ttgctttcaa aaacagtata
     7621 acacattctc attctttagc cgtgtggaat aattatgtga tagaataaaa acgaaaacta
     7681 tttcattaca attaggagga atcagctcat atgatcgaaa agataatttt aggaacctat
     7741 acgcgtcgcg aaagtaaagg aatctatacg atcgaccttg atacagaaaa agaagaatta
     7801 gccaaccttt cattagctgt tgaagaaaat agccctacct atgtagcaaa aagtaaagct
     7861 ggcaatcttt acacagttac tagtgtggat ggattaggtg gtgccggtgc gtacgataag
     7921 gattttcatt ttttgaatgc ggtaacagag tctggcgctc ctttgtgtta tgtagcggtt
     7981 gatgaagcac gtcaattact atacggggcg aactatcata aaggtgaagt aaatgtctac
     8041 catatattag gagatggcga actgaaagca gcagattcaa tattccatca agaagcgaca
     8101 ggtcctcata aaaaccaaga tcatgcgcat gtccactata cggatctgac tcctgaccaa
     8161 cgattagtcg tatgtgatct tggtacagat cgtgtgtata cttatgatgt gtccgaaaac
     8221 ggcaaattga atcttgtaac taccttcact gcagagccag gaacaggccc tcgccatctt
     8281 gtctttcatc caaatggaca attcgcttat ttatttggtg aattagacag cacagtccgc
     8341 gtgctttctt ataataaaga aacaggtgct tttgaagaat tacagaaaat atcgaccttg
     8401 ccagaagaat tcgacggtga aaatggaggg gcagctatcc gcatttcaaa tgatggcaag
     8461 ttcctttatg cttctaaccg aggacataac tcgattgctg tattcgcagt ttttgaagat
     8521 ggttcccaca ttcaaaacaa gcaaatcatc tcaacagaag gggattttcc ccgcgatttc
     8581 gcactagatc caagcaataa ttttgtcgtg tgtgccaatc agaacaccga taatctgaca
     8641 ttatatcgcc gtcatcctga aacaggatta ctggaattac tgaaaaaaga tgtctatgta
     8701 ccagaatgtg tctgtgttta ttttgatcgt tagtaagaaa aggagaatga aatgaatcag
     8761 caagacgtat tagacagact acgggaagaa ctaaatattc ctttctttga tgggaaaatc
     8821 gaagataaag agtatagcga agaggaatat cagaaactca aagccgattt acagaactac
     8881 tttgaacaat atgtacagaa tgtagaaaac taatcagctt tccatactat ctgaaaagaa
     8941 catcaggaca agacttttcc gtctatcctg atgttctttt ctacaaataa aaaaccgccg
     9001 agatggttag actcgacgga aaaggagtat ttactcgatt ttgagtaagt acaaggttgt
     9061 tgttggttat aggtatgtca ttatcataaa ggattttgat cactctatca taaaggtaat
     9121 attacattta tattagataa ctctagtttt tattgttatc aaagatttct tttatttttt
     9181 caacaacaaa ttagtcattt tttggatggc agctgtcaat tggtctgtcg cttggtcttg
     9241 ttttgcttca gatgtattga atagatcttc atctacccct gattcaagat cgatactttg
     9301 gatcatttct tcacagcctg ctacatatga aagataagct ttttcaaaat ttttatgaat
     9361 gcccattgct tttgctggtg gacgcaaagt actgactttc ataagcattg cccgatattt
     9421 ttttgttcct tcttcaaata acaggatgat ctcttcaaga gtcgatttgt ccagttcact
     9481 tgtttttcct tcatcaattg cgtttctgat cacttcatat ttcggatgca ttccttctcc
     9541 gattgcttct gtttccttga cgatttgatc gatggactgc acataatagc caatattcgg
     9601 taacatgttc gttttcctac tttctccatt gttgttatta tttattctac ttgtttttat
     9661 cctcaaatga caagtacttg aaaaaattta atggtttcta tacagctcgc cattcttatt
     9721 cgtgctcaaa gaaaccatct gctaaactat attgtttccc tgcgacaatg tcatattgga
     9781 tcaattgata ttcgtcgtaa atctcttgtt gcgagcgatt gaattgcgct tctttatacc
     9841 actgtccgtt ttgatagtac agttctcgtt caaacgccgg aagttcctgt tctaatgata
     9901 aaagcagctg ataaaagcca tttgttgtct gattcgtctg ttccattaga ttcgctgcaa
     9961 aataaaacgg actggtgatt gcatcgtgag tcgtttgctt ttctaattct cctttactgt
    10021 cgaacataag aaattgtgtt tcatgcaatg tttgtttgtc attttttgct tggatcgtat
    10081 ctgaatagat ccctggaaga tgatcccccc aaaaaacaac taaagtcctt ctagaaagat
    10141 tcttcaattc ttccgtgaac tgcttcagtg ccgtgctgct ataggaaata tcttgcagat
    10201 aattctctaa gtcaagtgtc ccacttccat ctgaagtttt tgcaggatag tctagattat
    10261 catactttcc atcgtagggc atatgtgtct gcatcgtgac taaatgaatg aactgaggtg
    10321 ttttttcttc cttgagaagt gtcattactt ctttgtaagc tgactcatca gaaatatatg
    10381 gattattctc tatcgtatcc gtataggtca tcgtacgctc gctgatgaac tggtcaaatc
    10441 ctaatgtctg ataaacatcc tctcttttat acatcgaagt attgtatgga tggatcgctg
    10501 tcgtctggta tgattgtgcg tttaacgctg aaacgataga aggaagctga tccatttttg
    10561 gtacaagcat cgtgtaaggg gtggtgagtt gcgcattaaa taacgccata gaaaaaccag
    10621 tcaatgcttc aaattcgata tttgctgttc caccgccata gttttgagaa agcatattgc
    10681 cactatatgt ttgatcagct acttcataat aatctgccaa tggctcacca gatacttcta
    10741 cgccgtttaa tcgactagga tctgagaaac tttcactcat cacgaacacg atgttcggcg
    10801 actcttcctc tacagtttta tttttttcat cggctagctt ctggtatttt tcagtgattt
    10861 ctttgatctt ttctttcgaa tatccttcag gttcatccat tggttctacc ttcagattgt
    10921 acaaaaaccc accgataaaa ccagtgttat agtagttcat tttctggctg taaggtatcc
    10981 atagtgccgt tttattatat gcttttcgca acaaattatc gggattgttg aaattactga
    11041 tataccctag cagtccaatt gccacaagca gtgtcgttaa tcggatcaat tggaatcgcc
    11101 ttcctttttt caggcttcga aacatgtacc agcaaaacaa tcctatcacg gttcctgcgg
    11161 cagctaagat cactgtaaac agcatagtgc cggtcatatc tttcagcaat ccgatttcag
    11221 tgatcatctt caaatcgtct ggataaatcg gttcttgccg ataactcatt ttcatataat
    11281 ttgcaaaacc taatacaccg atggctacga catacgtaag ggaacctaaa gggacagatc
    11341 ctgctagtgc aatcagaaaa ataagaataa tcagcaagac gagacaggct aagaagaatt
    11401 tttccgtatg ccaagaaaaa gcaaatttca acgcaaggtc tacggataga tcattttgac
    11461 accactgcaa aaataaatta ctaaaaacaa caagaaaaaa gatactgatg attgctgctg
    11521 ttccttttaa aatctttttc tgtttggtcg aaagtctgct tatatatctt ttcaaatgct
    11581 tgctccttca tttctatcaa taaaagaatg ttgcttctct tattttagtt gtacgaaatt
    11641 cgaaagtaaa gaacggaaag aagaattaat gaaatccaaa tattctcttt ctttatggcc
    11701 tttctggttt ctttaataag cggacctttc gtactccaga aattttttct agctgttgtt
    11761 tcacttgatt gagctgttca aaatcagtac tgtctatgtc aatcaatatg tagacatgct
    11821 cattcttatg gttacgcgat atatctgcga tcgctagctc gttctcattc agcagagaaa
    11881 atatctctgc taatatattg gttttctttt gataaaatat agtgaatcga aacggcgctt
    11941 ggaaaaacat tcgtgcggat ggataattga ccgcttctct aaccgtaccg aacaaaagat
    12001 aatcttttaa ggcttcaact gccaaacgtc cgctgtctgc caaagcttcc tgagtcgatc
    12061 cccctagatg aggcaacata agtatacggt cattttctaa gaactcttct tctggaaaat
    12121 cagtcaagta tttagccaga tgttgttttt ccaatgcttg cataacagca tatttatcaa
    12181 caatttcact tcttcctaca ttgataagta cagcatcttt tttcattttt tctatattcg
    12241 cctgatcgat caatcctttt gtatcttctg tcaacggcag catgactacg atgtaatccg
    12301 aggtagaaag aactgtttct aaatctgctt gccgaacata atccaactgg gcatcgcgga
    12361 tagaataacc caagacatcc atccctaagc tatagcaact tagcgctact tttgtgccta
    12421 tcgctccaag accaagaaga ccgatggttt tgccttgcaa ttctcgtccc acataagcac
    12481 ttcttttgtt ttctgcctgt tctaaaatat taggacctgt tagtgtttgt accatacgac
    12541 ttgcttcgat tatcggacgg gaagataaga gcaaacagca caaaacaagc tcttttactg
    12601 cattagcatt tacacctggt gtattcatga cgatcgtccc gttttcagaa gctttttcaa
    12661 cgttgatcgt gttcacccca acaccagctc ttgaaattgc tagcaaatct ggtgtaagcc
    12721 attcatcttt cactttcgta ctacgaataa ctattccttc ggctggcaaa tcatctatta
    12781 ttttgaaata ttccccttgt aacttttcta tctcttgttc tggaatagca tcaatcaaat
    12841 gaacgtgata cataaaaccc cctccttact aacaagtatc tcgaaatttc tgcaattaga
    12901 caactgttta ccatttgact tcctagaagc aaaaagagca gttagagcag agtaggctga
    12961 gtgataaaaa taaggctatg acaagttact gccataagcc ttattttcga gagcaaaata
    13021 ttgagggtga gatatcttga taaatggtgt tccgttagct gacattgata ataagactaa
    13081 atttcaaaaa agttgaaaag caaatttcgt caatctcttc ttatttctta aaactaagtg
    13141 gctttttcac aacctctttt tatctaataa ccactgtttc caactcaact aattttgccc
    13201 acgtttcaat ttgatctgtt gttagattta gcgagacaac ggtatgatga ccgccacctg
    13261 cctgaatcca tgaatggaca ccttcatgga aattcggttt agttttccat aacacacgtg
    13321 caacaggtaa attaggtgct tcagtcgttg gttcaaaggc ttctacttca ttgatcagca
    13381 gtttataatg cgtaccgaaa tctgccatcg atacgactac cccttctcct gctttcccat
    13441 caaatacaag acgcgccgga tcttctctat ctcccattga taaaggagaa acaacgattc
    13501 ttggtttcgt cgcagcaagc gtcggatcta cttccatcat atgggattcc aagattgctt
    13561 cttggccact agctagctcg taagtataat cttccatgaa tcctgtatct ttccctcgag
    13621 ccatgatttt cagcaaccga tcaatcgctg cagttttcca gtcgccttcc ccagcaaaac
    13681 cgtatccttc tgccatcaaa cgttgtacag ctagtccagg tagttgtttc attccataca
    13741 ggtcttcaaa attagtcgta aaagcgttat agcctccttc ttctaagaaa cggcgaatac
    13801 cgatttcctg ctgagcttgt acttttacat gttcctccca tgtcttttct tcataatcac
    13861 catagtcgaa ggtatacaat tccttgtact cttcaaataa ttgatcgact tcttcgtctg
    13921 atacgcgatc gattacttgt acaagatctc cgataccaaa ataatcaaca gtccaaccaa
    13981 attgaatctg tgcttccact ttgtctcctt ctgtgacacc cacgtttctc atgttgtcac
    14041 caaaacgagc cacttttatt ccaaaacttt cattataggc tactgcaaca tccatccaat
    14101 cagcaatctc tttttggatc tcaggtcgtt tccagtagcc aacgacgatt ttattttgtt
    14161 tatttaatcg agcattgatg aacccatatt cgcggtcacc atgagcggat tgatttaaat
    14221 tcatgaaatc catatcgatc gttttccaag gaatgctttc gttatattgc gtagctaaat
    14281 gaagcaatgg tttttgtaga agctttgtcc cgcggatcca catttttgct ggtgagaaag
    14341 tatgcatcca agtgatcaca cctgctactt cttctcgata attgacctct ttcatgattt
    14401 ttgtgatcgt atcagcagta actgctaatt cttgaaggcg gattggataa ggcaattggc
    14461 cattttcatt cagttcatcg accatttctt gtgcatgttt ctttacttct cgcagtgctt
    14521 cttccccata taaatgctgt gatcctacaa caaaccaaaa ttctttttct ccaatattca
    14581 acataattag ccactccttt tatttgtttt gtccgtaata cgcatttttt ccgtgttttc
    14641 gtaaataatg tttatctaaa atccgttgag gcagctcttc tgaaaatgaa ttgagctgtc
    14701 tggtaaataa attcatcttg catacttcat ctagtacgac tgcgttcatt accgcacttt
    14761 gcgcatcttt cccccaagta aatggaccgt gtccgtgtaa aagtacccct ggaattgcta
    14821 agggatcaat tttgcgttct ttgaatgttt ctatgataac ttttcctgtt tcatattcgt
    14881 acccgctgtc gatttcctgt tgtgtcaaaa atcgagcaca aggtaccgat ccataaaaag
    14941 tatctgcatg cgtcgtcccc atcgcaggaa catccaatcc tgcttgtgcc cagatcgtcg
    15001 cccatgtcga gtgagtatgg acgattcctc cgatttgagg aaatgaacga tataaaacag
    15061 catgagtagg cgtatccgaa gagggattga gtttaccttc aatgatatga ttgtccaagt
    15121 ccacaacgac catgtcgtct gctgtcatct cttcataact gactccgcta ggtttgataa
    15181 cgaaatatcg tgtttcagga tcaaaagcgc tcacatttcc ccatgtatat ttgacgagtc
    15241 cgtgtttagg caagtcaaga tttgcttggt atacttcttc tttcagttgt tccagcattt
    15301 tctatcactc ctttttcttg caattgatga atcgctgttt tttcaatgtc gatcccctca
    15361 cggtaacgtc tcataaatat ctgatagcct gctatttctt ctgctgaagg tgtgatggtg
    15421 attccttggt cttgtccaaa cacgtcttct gccaagaagt tttctaaaga cttcccatgt
    15481 cgatccatca tataggctgc taataacgcg attccccacg ctccaccttc tccggctgtg
    15541 tccatcactg taactggtgc ctccatagca gatgctagaa tccgctgtcc aacttctggt
    15601 gttttaaaaa tccccccatg ccccactaaa cgatcaattt ggacattctc agatttcaat
    15661 atatccattc cgattctcat tgcaccaaaa gcactggcta aatggatccg catgaaattc
    15721 gctaaatcaa aacggctacc tggtgtacgt acaaataatg gccttccttc atttactcct
    15781 gtaatgtttt caccggaaaa ataaccataa gaaagtaatc ccccgcaatc cgtatcgcct
    15841 tctagcgctt tgttgaataa taccgtgaat aatttatctc tgcttatctc catacccaaa
    15901 gaattggtaa attcttcaaa aatactgacc caagcattga tatccgaaga gcagttattc
    15961 gtatgaacca tgcctactag ttctcctgaa ggtgtagtga ccatgtcgat ttccggatgg
    16021 acattttcta gttctttttc taatacgatc atagcgaagg cggaagtacc agccgaaatg
    16081 ttccctgtcc ctttggctac actgttcgta gcgaccatac cggttcctgc atccccttct
    16141 ggcggacaaa ccgggattcc tggtctcaaa tttcccgtag ggtcgatcag ataagcccct
    16201 gattcgctta acttgccagc ttcttctcct gctactagaa ctttaggcaa aatgtgatcc
    16261 aagtcaatcg ctacgtgcgc tttttcgatt aactgaccaa aaatattttt catttcttga
    16321 tggtaggttt tcgttgaagg atcgatagga aacatgcctg atgcatctcc tacgcctagt
    16381 actttctcac cagtaagctg ccaatggaca taacctgcaa gcgttgtcaa aaaattaatt
    16441 tctttcacat gttcttcttt attcaaaata gattgataca aatgagcgat actccatctc
    16501 tgcggaatat tgtattgaaa tttttccgtt aatattcgtt ctgcttcttc agtgattgta
    16561 tttcgccaag tacgaaatgg tactagtaac tgttccttgc tatcaaatgc tagataaccg
    16621 tgcatcattg cactgaaccc aatcgacccg attgtcgtaa gattctcgcc atagatttcg
    16681 aatacttctg ctgccatttt tcggtaactg acgcgcagtc ctttccaaat atcttctaat
    16741 gaataggtcc agataccatt ttctagtttg ttttcccact cataactacc agcggcaata
    16801 ggagtaaaag atgaatcaat caatacagct ttgatcctag ttgatccaaa ctcaatccct
    16861 aaagatgtac gaccatctat gacatctaac atactttgcg catttacttc cttcataatg
    16921 atttaactcc ctccttctcg tcaaacctga tcaaaccgtc tttttagtag cgcttacaat
    16981 gattattgta tacttttgta cgtacatgtc aagcagttat ttacaagaat acaataaaaa
    17041 acacttgttt tgtacgtaca taagttataa aatgaaattg cttacaaaat ctgatcaacc
    17101 gccttctcga aattctagaa ggagagaata cttatgaaaa aagaaaaaaa agtttcctca
    17161 tcgtttattt atttttttgg cgcatttggc ggcattctat ttggatatga tattggtgtt
    17221 atgaccggcg ccttaccttt tttacagcat gattggggtt tggcaggtaa agcttctttg
    17281 ataggatgga tcacgtcttc ggtcatgtta ggtgcgatcc ttggcggctc tttatctggt
    17341 cttctttctg ataagcttgg acgacgaaaa atgattcttc tttctgcact tatttttatg
    17401 gctggttctg tcctttctgc atctgcccct cacaatggtt cttattttct gattgcagca
    17461 aggattcttc taggtttagc tgttggcgct gcatctgccc tagttcctgc ttatatgtct
    17521 gaaatggctc ctgctagact aaggggaaga ttgtcaggga tcaatcaagt gatgattgct
    17581 tctggcatgc tcctttccta tgttgcagat tatttgctaa aagggttgcc tgaaacgatg
    17641 gcttggcgcg tgatgttagg gctcgctgct gtccccgcgc tgattttatt tttcggtgtt
    17701 ttagctctac cagagtcacc gcgttttctt atgcaatctg gccgcttgga agaggcaaaa
    17761 agagtattga actatattcg gacaccaaaa gaagctgaac aggaattcga acaaatccag
    17821 ctgaatgtca aacaagaaaa gacaaccgga acttcttggc atactttgtt tttagaaaaa
    17881 tatcgttcct tagtcattgc cggtattggt gtagcagtat tccagcaatt tcaaggagct
    17941 aatgctattt tttactatat tccgctgatc gtagaaaaag cgactgggca tgcagcgagc
    18001 gatgcattga tgtggccgat catccaagga atcattttag ttgctggttc tttgctcttt
    18061 ttggttattg cagacaagtt caatcggcga actttattga aaataggcgg tagcgtgatg
    18121 ggactttctt tcatcctacc agctgtttta ggaactgtac tggatgctca tacaaattcg
    18181 ttgttgattc tcttgttttt atgtatttat gtcgcatttt attcttgcac atgggctcca
    18241 ctgacatggg taatcgtagg cgaaatattc ccactagctg tccgcgggcg agcatctggc
    18301 ctagcctctt cttttaattg gattggttct ttcctagttg gcttgctttt tcctgtgatg
    18361 actgcttcta tgtctcaaga aatcgttttc ggcatttttg ggatcatctg ctttttaggg
    18421 gttttattta ttcaagagat cgtaccagaa actcgtggga aatctttaga ggaaatcgaa
    18481 caaagtgcat ctaaaaaaac ttatcctaaa cgtatttcta tccatcattc ttaaagaggt
    18541 tgtgacagaa gtggtcagct tcaagaaata agaaggaacc gcttagtttc cagtcacaag
    18601 gaacaatttc aaaaaacagc ttctcatgtt tgatgaatat tgtgacttgt gaccagttgg
    18661 taggagaaca ccgtttagtg gagaggtggt gaaagaaacg ttcagccttg agcgatgaca
    18721 aaagctctca caaacattgc gacgagtaac cgtaggagca ataagaggcg gtaaaaaccc
    18781 gtctcttatt tttcgttttc tttcactgtt tcccgttcaa ctagtacagg ttcatagaga
    18841 atatttggct gtgcctgtcc tttttcaatc gttgtgacga tccaccgtgc ggcatcttgt
    18901 cccaactttt cttttggatg cgtcaatgtt gtcaattttt tattccctaa atggcttaaa
    18961 gaagaatcgt ctgttcctac gatcgatagg tcatctggaa tatgatagcc attttctgtc
    19021 aacttatcta gcattaaact agctatttga tcgttatagc aaatgatgcc atctaaagct
    19081 tgttcttctt tcaaccgttg tactgttttt tcagcgattg tttctcgtgt ttccgtggtg
    19141 aaagtaatga tatcttccgt caaaaaacgc acattatact gttcacacgc gctgataaaa
    19201 cctttcatcc tgtattttcc ttgaagatcg tcgatttttg tgatgaacaa cagacgctga
    19261 tgtccttttt ttatcaaata ttctgctgcc ataaaaccga ctttcacatc atctaaacaa
    19321 atataagagg catttaactc ttcataagca gcattgatca tcaccatcgg aatgccttgt
    19381 tctctcaaag caacataaag tgctaagttt gggttatact gattactttt cgtaggctca
    19441 acgatcaaac catctacccc ataagcaatc attttcttca aacactcttt ctccaattcg
    19501 tgatcgttat tcgtactggc taaaagcaat gaatacccag attctcgtaa ttcattttcg
    19561 attcctcgga taatcgacgg aaaaatataa tcagagagat atgtggtgat gacaccgatc
    19621 gtttttgttc tgttcaatgg tgttttttct ttcttatagc tgtcatctac atatgtgccc
    19681 gacccttttt ctttacgcag atagccctca tttaccaaca cagcgattgc ttgccgaatc
    19741 gtatgtctgc tgacgtcata tatttcttgc agcttgaatt ctgagggaat ggttgtattt
    19801 acttcgtatt cctcacgaag gatcttttgt tttatctctt ctgcaatatc ttgatatttt
    19861 gccatttgcc ttgccccttt ttatttgtac gtacatatat cataacgagt ctgcaaggtt
    19921 tattcaagca aaaatcaagg tttcttgtgc tcataaggca tacggaacag gttcaccaaa
    19981 ttctggaaat ccttgctcat tccacgtaaa gcattgcgcg ttcgcgtgcc gattaggatt
    20041 gtctaacgga tccccttttt ggttcttctc tggtcgagca tgatagatca aaatatcaaa
    20101 gcacccgtct tctgaaaccg taaacgagtt gtgaccagga ccaaattgct tatttttttt
    20161 agaagaaacg aatactggct ccgataattt atgccatgat tcttttaata aaagattctt
    20221 gtgctcatca gcccacagca ggcccatggc atagttttca tctgttgcac taccagaata
    20281 cgtaatgaat attttcccat tgcgagtaag gactgctgga ccttcattta ctgaaaaacc
    20341 ccgtttttcc cattcatact caggtataga aaggaggatc tgcttcccct ttaacgtcca
    20401 aggattatcc atttcggata gataaagatt agaattccca gtaatttcag gatcttgttg
    20461 ggcccacaca taatatagct ttccttgaga agagaaagtt gttccgtcta aactgaaagt
    20521 ttcccttttc gtttttattt gtcccttttc tatccaatct gtattcattg gatctatatg
    20581 ctgattttca agtacgaaca tgcggtgatg atgagattca tcacgtatag ttttatgatt
    20641 gcttgcagca aagtaaatat accattttcc attgatataa tggacttcag gtgcccagat
    20701 caattcactc atcggtcctt cttcatgagc ctgccataca atcaggcttg cagcatgttc
    20761 taattcattc agtgactttg cccttcttaa ttcaatcact tggtagcctg gaaccgatcc
    20821 tgtaaaataa taataaccat ctgtatgttt atatacccaa ggatctgccc gttgccgaac
    20881 gataggatta ttataaagtg tcttcatcat aacaaatttc ctcacatttt ctttattcta
    20941 attacattcc aagagtattt ttctatccct acatttaatt cgtcatgttt ttctattgaa
    21001 aaatagttta tttcaggttt tattttttct ggttcttcac gtgtattttt gtccaacaga
    21061 tcgctactgg ttaatgtgta gtgctctcct tgatctagca aattgaaatc atgttcaatc
    21121 gttatctttt gttgttcatt tgtgcgattg atcaagaaaa tagtcagctg attgccgtta
    21181 tctacgatca cactgtccac ataaggaaca ttttttatct tttcacaatc ataaacaggg
    21241 caaatgattt gttgtttcaa tacttgacct ttagcatatt tcgataagtg ttgcatcaca
    21301 taataagttg gattcctcca tgcttgtcct ttagcatctg tcagtattaa aggtattgtg
    21361 tttactaaca atgattgaca actaatcttt acgcgatctg cgtgtcgtaa aatagccagc
    21421 cctaatgaac caaataataa tgtatcttcc atagtaaagt aacaccaatc gatgggacta
    21481 ccgacttcaa aatccctatg tcttttttcc ggttgggcat gtacattcca ttcatcaaac
    21541 gctagataca ttgttttctc acttctcaaa tatcctttta cataatcaca agtagcaacg
    21601 atttcttcaa tttgacgatc gaatcgataa gcttttgcaa ggtatgtcgc tgtatcatca
    21661 cgctcatttt taggaggttt agtcaagtct tcttcatcat acttatcaat atagttatgc
    21721 aaagctaaat aatctacatt ctcataagct tcttctaatg cgatacgatc ccattcaggg
    21781 tacgatggta aacgtggcgt agagcttcca actaagattg tttcaattga atcatccact
    21841 aacttcatga cttttgaggc ttcatttgct aacctaccat attcataagc actcttagcc
    21901 cccacttgcc atggaccgtc tagctcattc cctaaacacc ataatttaac gccaaaaggc
    21961 tttttttgcc cattttttat tcttaaatca ctccagaagg ttccttttgg aaaattacaa
    22021 tattctacta aattttttgc ttcatcaatt ccttttgttc ctaaattcac cgtgaatatc
    22081 ggtttcgctt gtacatgact gatccattta aaaaattcgt ggagtccaaa agaatttggt
    22141 tctatacctc gccatgctaa atcaattttt tttggtcgtt gttctttcct acctacagta
    22201 tccacccaat cataaccaga tgtaaagttt cctccaggat agcgaatgac acctaattct
    22261 aattccttta cataagagag gacatcttgt ctaaacccgt caccatcagc tgtttcatgg
    22321 cccggttcat aaatccctgt atagacgaca cttcccatat gctcaacaaa tgagccaaac
    22381 agccgagaac tgatctctcc taccgtaaaa tcctctctga tttttacttt cacttcttcg
    22441 tccaacagct ttccctacct ttcatttcct atagtcaaga taatagatac aagtttccat
    22501 acgccaccag caaagataaa aataaaagga gggaaaagaa atatgagcag tacaaatcct
    22561 aataacaata cgcaacaaag aatatttctc aaaaaatcaa aaaatacttt ccatgcactt
    22621 tgttcaaaaa tttgtaaaaa tgtgatatct ttgttttcag aaatcacata tatccaggat
    22681 aaaagaaaca tggaataaat aagtaaaact acatatccac taattgaaag tacgattgtc
    22741 tgttcttcta ataattgatt aacaagaaaa taaataatga tcgttaacag actaaggaac
    22801 aagttcttac caaaatgttg ctttattcct tgaaagaaaa aataaatgat atttcctgat
    22861 ccctcacctt tccattgcgc cattacataa aaaacagcac atgtagcagg cacgatagta
    22921 ataacaggca aacacacgat tacccagaga atacctaata agatgtaatc agaaaccaca
    22981 atcaactgtc cataaagttt agtctcaact attttcatct tcctcactct ttcattccag
    23041 acatagctgt tccttgaaca aaatatttct gtgcaaaaat ataaaccact agtaatggca
    23101 gcattgaaat aaccgttcca gccattacag gtccataaaa agaaatgttc tgccctgtaa
    23161 ataaagctaa ccctgctggt aaagttctca tttttgaact acttgtcaat atcaatggat
    23221 aaagcaagtc attccaacta ttcatcagcg tgaaaatccc taatgataaa agagtaggtt
    23281 tgctcaatgg caacatgatt ttcaaaaaaa tattaaactc acttaatcca tcaatgcgtg
    23341 ctgcctcttc caaagaatct ggtaaagtaa taaaaaagga acgcatcatg aagataccaa
    23401 atgcagttgt catacgagga atgatcaaac ctgcataagt atcaagcatg ccaagcaaat
    23461 tgacttcaat aaatagagga atcatgaaga cttggaatgg gatcatcatt gtcagcaaaa
    23521 cgaaatagaa caaaactgat tttcctttga aacgcatacg agcaaatgca taccccgcaa
    23581 gagaatcaaa gaatacagaa gtgatcactg tccctccagc aaaaatcact gtatttttga
    23641 tgtaatctaa taaagggatc gttgtccaaa ccttaatata gttatctagg gtatagctat
    23701 ctgcaaatat tctaggcgga aatgagataa tgtctttttc gtgtttaaaa gaagaaataa
    23761 tcagccacaa aaaaggaaac gcaataacca acaatataac tagcatacta atcgttcgaa
    23821 tgattttttt accactaact gcttttcgac tattcacagt aactgtaatc gagctttctg
    23881 gtgccataca ggtttccctc ctatttcatt tgtttttctt tccgtgacat gtacacatta
    23941 gtggagatag ctatagttgc aacaatgata aacaaaaccg aagacagtgc tgatgcatag
    24001 cccaattcat aaggtgctgt aaatcctcga ctgtaaatat attgaacagc tgtttctgtc
    24061 ttaaattgtg ggccaccagc ggttgcaaca tatacttggt caaacacttg catcgaacca
    24121 atcagattcg ttaaaataca aaaacctaag gagggaacga tctgaggtag cgtaatgtta
    24181 aaaaactgct gtcgatttgt tgcaccatcc atttgagcag attcatacaa agagggagag
    24241 attccctgaa tagcagtcaa gagaatcgtc atagtaacac caaaattctt ccaaacagtc
    24301 atgatggcta ccgtcggcat agcaagggtt ggatcgcgaa aaaattgagg gatcggcaag
    24361 ccgacttgtg aaaacaagta aggaatcgcc cctacattcg gatcaagcaa catagaaaaa
    24421 ataatcgcaa ttgacgtcaa tgaacaaata actggaatgt aaaatgtcga acgacaaaaa
    24481 cgattgaatc tggtattttt agataatgct actgctgcta ataaaccaac gataatttgt
    24541 gttggcgttt cgagtacggt gaaatataaa gaatgaatca acgaattaac tgctcgttca
    24601 tcactaaata tacgtgtgaa attttcaaaa cttacaaaat ttcttgatgt aaagaaaata
    24661 ttcaaatctg taaaactaat agcaaatgtt cctaaaagcg gtatgacagt aaataataat
    24721 aaaactaaaa aagatggagt taaaaacaaa tacgcagcac gatcaggacg attccaatat
    24781 ttggtgaatc cgttcatata gtccacctca tctttcattt tgataaaata tacaatcttg
    24841 gtttaaacga atctgaataa gatatccgaa caatcgctgt gatttttgca ataacaccta
    24901 tctgcaaata ctaggtcgat cattcagata tctgtatctt gccaacagtc tatcctgtat
    24961 aaagttctgt actatttttg cagtaattga ttgatggcat catctgcatc gttcatgagt
    25021 gtctcaggat cttctcctgc taataatttt tcaatcagtg gattgatgat atcgttattg
    25081 atcgcagaga tttgggtaaa acctggtaaa tatggctctg catagtccat ttgtttcgat
    25141 aattcagata caataggatc attttttact tcttcatctt ttgctactga atccaagtag
    25201 gcaggaaaac cattttcgat actccatttt ttaccaattt cagttgtatt ccaatatttc
    25261 aaaaactcat agacagcttc ttttttatta ttatctgtac ttgccggaat ggcatatcct
    25321 acgccatcta aaatagctgt ttttttgtct gaatcactcc cttgtggtaa ggtagtcaca
    25381 ttataatcaa tctcgtttgc tttcaatccg ttatttaacc atggcccatt gatttcaatg
    25441 gctagttgac cagcattcat taaattgtcc atttcggcgc cagaaatcga ttctggacca
    25501 gtcttgtcta catggatcat tttttggatc gcttttaacg ttttaaggtt ctcaggagac
    25561 gcaaattcag acttagtata atcatcgttc actaatttac cgccatttgc taacatccag
    25621 ttatataacg gtgcagttcc atccttattg aagacaaaac ctgatacatt tttactacta
    25681 tcagttaatt ttggtgccat ttctgtcaat tcttcccaag tagtaggtgg ttgttcagta
    25741 tctaatccag cttgtttaaa caaatcatta ttccaataca tatacatgcc ttgtacctgc
    25801 atggggacaa aatactgttt accttctact tcacctaatt caatagccgt ttctttaaaa
    25861 tcagatttat ctactccatc atattcccag aaatcgtcta atggttgtac cgctccgttt
    25921 tctacatact gtgcaaaatc tggataattt aatgcaataa agtcaggtgc ttttttagaa
    25981 gaaatcgctg taggaagttt ttcattcaaa ttggcccaag tcatgatatc catttcaatt
    26041 ttgatgtttt tatcatttgt tttattaaat tcattaacga tttcttgcaa tatttcacca
    26101 tcagatgcag taaacccatt ccaaaaagtc aaagtcactt ctttatcaga atcagattct
    26161 gctgaacttc cgccaccgca agctcctaaa atacctgttg cagctaccaa caaagagcca
    26221 attaaccatt ttttaccttt cattttttgt tcctccttca tctgataaac gacatctgac
    26281 cacattccaa gatagcggtt tcaataaaca attgattttc ccatcttcag cttttacatt
    26341 tgttgaatga cttggtttta cttgttcact tttaggaaga ttagttgctt ttgtatcaaa
    26401 tccactcatt tcagaaaaat caatcactcc ctctaatgat aattcagaca agtgaacagt
    26461 tagattcatt tcttcttctc cacggttaac tgcaaatatg acaagttctt tgtcatttat
    26521 tacggctata ctttctaaat acggcacttc ttcaaaatct tccgtcgcat agtttgctga
    26581 acgaatttgt gggcttaaca cctctccttg tccatacaca gcagcttgca taaatggata
    26641 atagatcgtc tgtctccaag cttctccgtt tgtttctgtc ctgattggtg caataacatt
    26701 taccaattga gcaagacaag caatctttac tctatctgca tgtttcaata aagtgattaa
    26761 tagacagcct aatagtaatg catcttcaaa attatagtta tcttctagta aaggaggagc
    26821 aatctgccaa ggcgctgctt gctcatcttg ttccgctgaa tgataccaaa tattccattc
    26881 atcaaaggac aggttgatct gcttcttact atgtttcttc gccttgactg tatcgcacat
    26941 cgctgtcact tcagaaatga accgatccat gtctaaggaa cgtgcaagat aatgagaaag
    27001 atcatttttt tggttgccat aatattgatg cagagacaaa taatctattt gatcgtacgc
    27061 ttgatcaaga actgttaact cccaatcacc aaatgtaggc atttgccgat tcgaacttcc
    27121 gcataaaacc acctcaattg aatcatctac gagtttcatc gccttagccg tttcctctgc
    27181 taaccgtcca tattcttccg ctgttttatg tccaatctgc caaggaccgt ccatttcatt
    27241 ccctaaacac catgtcttaa ttccaaaagg gctctcttga ccattcgaac gccttaaatc
    27301 actccaataa gttcctttag gaaaattaca atattcgact aaattacgag ctgcatctac
    27361 tccacgagta cccagattga cagccatatt gatttcggcc cccacatgct gacaccagtt
    27421 ataaaattca tgaatcccaa cttcattcgt ttcaattgtc cgccaagcta aatccaacct
    27481 tctaggtcta tttgctatgg gcccaatact gtcttcccaa cgaaatcctg acacaaaatt
    27541 tccaccagga tatctaatca tgggaatgtt caattctttt accaagtgaa tgacgtcttg
    27601 gcgaaatcca cttttattag ctgatggatg atctggctga taaatccctt catatactgc
    27661 tcgtcccata tgttcaataa atgaactgta caaccgcgga tcaactttgc ctacacgctt
    27721 gacactacta atcaacattg tggcttccat aactgacctc ctttaattta tacatgaata
    27781 atacacctcg ttgaaagcgt tgtcaataat ataaatgtta aacttactta ttttttatcg
    27841 attttaatta accaaaatat taataatgat taaaacaaat aatttaatat aacttttatt
    27901 aacaaatatg ttaatatata agaaaaacct tttaagaggt gatatatatg caactacaaa
    27961 taaacgaaga atccttaccg gtatatgaag cattagctag taaaacaagg atcaagatca
    28021 tccagctact ttccaaaaaa aagatgaatg tcaaagattt ggcaaaagaa cttggagtga
    28081 gcagtgctat cacgacgatg cacgtgaaaa agctagagga agcaaacatc atcaaaacag
    28141 aaaaagtagg acaacaaaaa atttcgagcc ttcgtgtcga taaaatcgat atcagttttc
    28201 cagaaaaaat attcaatgct tttgatacga aagaaacatc gatacctatt ggtcactata
    28261 caaattatgc aattgaacca acatgcggtt tagctacgat ccatgatttt atcgggaaag
    28321 tggatgaacc acgctatttt atggatcctc gaaggatgga tgcccgcatt ctctggttta
    28381 ccagcggatt tgtcgagtat caggcaccta attttttaaa tacagaggat acattggaga
    28441 tgttggaagt ttctgtggaa atttcttctg aatttccttt ttcaaatgac aattggcctt
    28501 cagatatcac tttcagccta aatggtgtgg agctaggaac atggacgagc cctggagatt
    28561 ttgctgatat ccgcggtaaa tatacgcctg attggtatcc agataatctt aatcaatacg
    28621 gcttgctgaa gacgatccga attaccaaac acttgacgaa tatgaatgga gaacccttgt
    28681 caaacatcac gatcaacgac atccctaaag agcaagacac ttggcatttg cgaatcgaag
    28741 tgaaagacga tgccaaacat gtggggggct gtactttatt tggtaaagga ttcggtaatt
    28801 acgatcagga tatcaaatta aaagtttact acagctgata tttttctttt ctattttata
    28861 aaacaaaaac agccaatccc tatgaaggga cggctgtttt tgctttattg ttatttccag
    28921 aaatcatcca aaacggaaat tggcaaatgt cgtttatgtt gtgtcttgtt ccaccatgct
    28981 tcgatttttt cttgtgcttg agcgggaact tcttttcctt ctagataatc atcaatttca
    29041 ttatatgtga cacctagtgc gacttcatct gcaatcattg gtttcccatc ttcaagatct
    29101 gcagtcggaa tttttgtgta taatttttcc ggtgcattca atgcttgaag caattgtttc
    29161 ccttgtcgtt tgtccaaacg gaataatggc aaaatgtcgg ctccaccatc accaaatttc
    29221 gtgaagaatc cagtaatgtt ttctgcagca tgatctgtcc ctaatacggc tcctgctctt
    29281 tctccagcta cggcatattg cgtaatcata cgttgacgtg ctttgatatt tcctttgtta
    29341 aaatcgctga ttcccacacc tgcttcgctt aatacttgta cttgtgcatc aacagctggt
    29401 ttgatattta ctcgcagact aacatcgggc tggataaagt caagagcagc ttttgcatct
    29461 tcttcatctg cttgttcacc ataaggaaga cgaacagcga taaattgata gttttcatct
    29521 tttgtttctt cacgcatttc ttccatcgtt agttgcgcca aacggcctgc taaagtagaa
    29581 tcttggccgc cgctgatacc tagtacaaaa gttttaagaa acggatattt tttcaaatat
    29641 gctttcataa agtcaatact tttacgtatt tcttcttttg gatcaattgt tggtttgaca
    29701 cctaattcat tgataatttc tgtctgcagg ctcatttatt ctcctcctat gcttctgtat
    29761 gaagttctgc tacgcttttt ttcattcgtt ccatgatagc cattttatgg ttccagcatt
    29821 cagtagacag atcgactggg tatttttgcg gattcaagtc tcgtttatac tcttcccata
    29881 gtgaatctag attttctttt gtatattctt tgatttcgtt tagtgcaggt aattcgtaaa
    29941 ctctttttcc ttcgataaag atatcttgta aaactgggcg agctgtaaag tctcgaacag
    30001 ttttattgat aaacgtatgc actggatgga acatgaagat agcttcttct tctcgagggt
    30061 cttcatccca taatgtcacg tagtctcctt cagatttccc atcaaaattt cgagtgatgc
    30121 gccacacttg ttttttccct ggagtagtta ctttttctgc attgcttgat aacttgatcg
    30181 tatccatcat ttttccttca tcatcttcga tcgatactag cttgaatact gcaccaaggg
    30241 ctggctgatc ataggctgtg atcaattttg tcccaacacc ccatacatca attttagctt
    30301 tttgcatctt taaattcaaa atcgttgctt catccaagtc attcgaagca tagatttttg
    30361 cttccgtaaa tccggctgca tctaattgtt cccgaacacg cttagatata taggccatat
    30421 ctccactgtc gatgcgtacg ccttggaagt tgattttgtc acctaattct ttcgccacgc
    30481 gaatcgcact tggcacacct gatttcaacg tatcataagt gtcgactaag aacacgcagt
    30541 ctttatgggt tttagcgtag gccataaatg cttcataatc atttccataa gattggacta
    30601 acgaatgagc atgggtcccg ctagctggta tcccaaatat cttcccagca cgtacattgc
    30661 ttgttgcatc tgctccgccg atatatgccg ctctagttcc ccagactgct gcatccagtt
    30721 cttgtgcccg tctagttcca aattccaata gtggatcttc tccgatgaca gatttgatac
    30781 gagctgcttt tgtcgcgatc aacgtttgga aattgaccat attcaacagt gctgtttcta
    30841 ctaattggca gtgcgctaaa ggcccttcca cttggattaa tggttcattt gcaaaaacta
    30901 gttctccttc acgagcagaa cgaactgttg ctttaaattc aaaattttgt aaatactcca
    30961 aaaaaccttc aggatagact tctaatccac gcaagtaagc aatatcggaa tcattgaacg
    31021 tcagattctc taaataattg actaaccgtt ccaatcctgc aaaaaccgcg tatccgtggt
    31081 taaaaggcat ttctcgaaag taacattcaa aaacagcatg taaatcagct cgacctaatt
    31141 cccagtatgt ctgcatcata ttgatttgat atagatccgt atgcagcgtc aaactgtcgt
    31201 ctgggtaagt attattcatc ttgtattact cctcttggca agttttcatt tcctacccat
    31261 tataacaaaa aaaattaata ggaaaagaaa tcttccattt tttctgaaaa agcacatgtt
    31321 ttacggatat ttcttgctaa aaatgaactc catctgctaa aactatcctt tcttattggt
    31381 atttaaccta aacgagaaag aatacttatt ttattcaatg tatccgagac acgaagaaat
    31441 tagcgttttc tatttttctt acttgataaa ataagtagat tttcctacta caatcgtagc
    31501 cgttaagata ccgatgattg aacaaatata accaaatgtc tctacaacat aatcaatggc
    31561 aaaagttttt gaagcaataa tacgtctttc cttttttgct tcttatcccc aaagaaacgg
    31621 atataactta ttatgtattg aatttttttg gaaaggcgaa ttatgacaag atataccttg
    31681 cgttgaaaaa actcgcggaa aaaatatcac acgtttttaa aaagatacaa agtaagaaag
    31741 cagctgtcaa tagaatggaa acaaaaatgg cgagacatca atcagtctcg ccttgttttt
    31801 tattgtatac attagatatg gagcaggtta ccataaagat gattattagt cgttattaaa
    31861 tagaatcctt cgtatttttt tgttctatca ctacaatatc tttatttgga cgtttgaatt
    31921 gtattccggc acgttctagc tcctgtttca ctcctacatg gactttgaca tcttctcgtg
    31981 tcatttttaa aacgtgatga atcgcatagt cgatttcttg ccttgtgatt tttttcttcg
    32041 tattgatcat cacgacttgc tttttatcat atgtgtctac caatagcagt tgtgttcgat
    32101 ctaacgaaaa taatttgtaa taattaatag ccgtattccc aaaaaagaat tccactactt
    32161 taggataaaa tgtcttaata tttaaattac tattcacagg tgtttcgatt agcttcatct
    32221 cattcctctt ttctatatag tcgatttaga tccaatctta agttatgtta ccatctttcc
    32281 tagcattaag gaatcggtta caattgaacc tttaaagaca aaaggattta ccttttatca
    32341 cttaatagta taacataatt tcgtgagaaa aaaatacaat tttttcttga ccatctcgtt
    32401 acgtaaagga ttatgcttac atttgtcagg aggaaaaata atggagtaca caataaagaa
    32461 aatgtcagag atatcaggag tcagtccgag aaccttgcgc ttttatgatg agattggact
    32521 gctaaaacca gctaggatca attcttcagg ttatcggatt tatggcaaaa aagaagtcga
    32581 tcgtctgcaa cacatcttat tttatcgaac aatggcattc aagttggatg atatccaaga
    32641 ggtcttagac aatccatctt ttgaccacca gaaagcttta atcaagcatc agcaaatgtt
    32701 gctggaaaaa cgggcacaaa tcgataccct cttgacaact gtccaacaga cacttgatat
    32761 gtacgaagga ggaagaaaaa tgagtgacaa agaaaaattt gaagggttta aacaacaaaa
    32821 gttgaaagaa aatgaagaat cctatggaca agagattcga caaaaatacg gaaaaaaaaa
    32881 ggttacagca tccaatgaaa aatggatgaa tatggataaa gaaacgtatg aagaaatgca
    32941 aactgttgaa aagcagatgt tatttgatct cactctttac ttaaaagaac cagaagatga
    33001 aagcttagca gaccgtatct tccaagctca taagaagtgg ttctcttaca gctggcctga
    33061 atatcagcca aaagcgcata aaggactagg aatgatgtac gaagccgatg aacggttcac
    33121 tgcatattat gacgagcgaa gtggtcatgg tgcagcaaaa gctttaaatg aaattatcca
    33181 aaaaaaggcg taatctttta catgatccaa caaaaaagta cacgtaaaat gacctaataa
    33241 caaaacagca gacacccaag ttagattttt atgctaactt ttggtgtctg ctccattctt
    33301 tcacaagttt tttcatccta ctttaaatat attcatcgtt gaataaacgt ataaggtatt
    33361 ttcactttat cctttttttc tgtaagaaac aattgaaatg ccatttctcc gcaagcttcc
    33421 agatggtggt cgatggttgg gaaaccaaga agctcgctga tcagcaaatt atcgcgtcca
    33481 atcaccactg gtgtcttatc ttgcagatag ctttgcaaga tgcctgctgc aaccatatcc
    33541 gaatttgtaa agacaccatc aactccaagc tctttaaaaa agaagcctgc ttgttttcct
    33601 ttctctgcat ctatacaatc ccaaaaaaca agtgattcat caaaagatgg aaaaagttga
    33661 tgacatagct gcaaagtgat ttttgagtta taacttagcc tgccacttcg ccccaacgtg
    33721 atgcccaatt gctttatacc ttgctgcttc aaataagtaa gaccttctgt aattgattgt
    33781 tctcgatcaa taaagacaca gcttgcctgt tttcccttga tttcttcaca aaaaactaga
    33841 ggtccatatt tttgatatgc caataaatca tctagtgtat tggcacgaga cgtaatgatc
    33901 aatccatcga aagctttagc tgcaaattct tcaagatacc ttcgctcttt ttctttatga
    33961 tagcccgtcg gtaaaagtgt cactttgtaa tcataatcaa acgctgcttc aatgatccca
    34021 ctgatcaact gatcaaaata cggatgattg gtataaggca gaatcactcc aatgcttttc
    34081 gtttttccat aacttaactg tctggcattt gcattaggga tataatcgac ttctttcatg
    34141 accgcaagta tttctttcct tttttcctca tctacatatg gatgtccatt gattacacgt
    34201 gaaatagtag aaactgaata acctgtgagc tttgcgatat ctcgaatgtt tgccactgct
    34261 gcttcgccac ctatacaaaa agattttctt gtaaatagtc taccacagct ggaccgcatt
    34321 caaaaaactg atacattttt ccttcatgcg aaaaactgat ccgctgcccg ccccaatata
    34381 tatcttttgt caaattttcg atttcattca ttggtatttc ttttactata ttcctattca
    34441 acccttgaaa actgtacaac gttaatacgg atcccttgaa ctcgacaaac gcataaaaat
    34501 aattgttcgt tgtgccatct gttatctcta cataagtatt cattgcaagc cctccttttg
    34561 atactttgag tatagtgtat gaaacgcgtc tcatagcaag gagtttgttt tgccaaagaa
    34621 aagttttatt cctctttcat tattaccaaa gtctcttatt ttaaattgaa tacattataa
    34681 tgatactgta aatatatctt tattattagt gaat
//
LOCUS       DAZAFF010000018        34530 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000018 DAZAFF010000000
VERSION     DAZAFF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.018"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG9BB_000890"
     CDS             212..604
                     /locus_tag="ACG9BB_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN2836857.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG9BB_000891"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG9BB_000891"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFN2836858.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG9BB_000892"
     CDS             2698..2943
                     /locus_tag="ACG9BB_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836859.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG9BB_000893"
     CDS             3165..4775
                     /locus_tag="ACG9BB_000893"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFN2836860.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG9BB_000894"
     CDS             complement(4752..4964)
                     /locus_tag="ACG9BB_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836861.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG9BB_000895"
     CDS             5067..5936
                     /locus_tag="ACG9BB_000895"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFN2836862.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG9BB_000896"
     CDS             6101..7372
                     /locus_tag="ACG9BB_000896"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFN2836863.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG9BB_000897"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG9BB_000897"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFN2836864.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG9BB_000898"
     CDS             8763..9026
                     /locus_tag="ACG9BB_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFN2836865.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG9BB_000899"
     CDS             9471..9920
                     /locus_tag="ACG9BB_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2836866.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG9BB_000900"
     CDS             9977..11395
                     /locus_tag="ACG9BB_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFN2836867.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG9BB_000901"
     CDS             11395..12255
                     /locus_tag="ACG9BB_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFN2836868.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG9BB_000902"
     CDS             12429..13202
                     /locus_tag="ACG9BB_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN2836869.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG9BB_000903"
     CDS             13349..13714
                     /locus_tag="ACG9BB_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFN2836870.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG9BB_000904"
     CDS             complement(13862..15070)
                     /locus_tag="ACG9BB_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2836871.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG9BB_000905"
     CDS             complement(15089..15367)
                     /locus_tag="ACG9BB_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836872.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG9BB_000906"
     CDS             15560..16216
                     /locus_tag="ACG9BB_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN2836873.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG9BB_000907"
     CDS             complement(16658..17455)
                     /locus_tag="ACG9BB_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFN2836874.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG9BB_000908"
     CDS             complement(17452..18636)
                     /locus_tag="ACG9BB_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFN2836875.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG9BB_000909"
     CDS             complement(18661..19908)
                     /locus_tag="ACG9BB_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFN2836876.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG9BB_000910"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG9BB_000910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFN2836877.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG9BB_000911"
     CDS             21888..22202
                     /locus_tag="ACG9BB_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFN2836878.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG9BB_000912"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG9BB_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFN2836879.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG9BB_000913"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG9BB_000913"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFN2836880.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG9BB_000914"
     CDS             23546..23875
                     /locus_tag="ACG9BB_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFN2836881.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG9BB_000915"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG9BB_000915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFN2836882.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG9BB_000916"
     CDS             24863..25687
                     /locus_tag="ACG9BB_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFN2836883.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG9BB_000917"
     CDS             25680..26027
                     /locus_tag="ACG9BB_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFN2836884.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG9BB_000918"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG9BB_000918"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFN2836885.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG9BB_000919"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG9BB_000919"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFN2836886.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG9BB_000920"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG9BB_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFN2836887.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG9BB_000921"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG9BB_000921"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFN2836888.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG9BB_000922"
     CDS             31684..32451
                     /locus_tag="ACG9BB_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2836889.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG9BB_000923"
     CDS             32441..34483
                     /locus_tag="ACG9BB_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2836890.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11104 a         6123 c         7250 g        10053 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc
//
LOCUS       DAZAFF010000019        34469 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000019 DAZAFF010000000
VERSION     DAZAFF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.019"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACG9BB_000924"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACG9BB_000924"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFN2836891.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACG9BB_000925"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACG9BB_000925"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFN2836892.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACG9BB_000926"
     CDS             complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACG9BB_000926"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFN2836893.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="ACG9BB_000927"
     CDS             2179..2499
                     /locus_tag="ACG9BB_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFN2836894.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3468)
                     /locus_tag="ACG9BB_000928"
     CDS             complement(2611..3468)
                     /locus_tag="ACG9BB_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN2836895.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="ACG9BB_000929"
     CDS             complement(3576..4880)
                     /locus_tag="ACG9BB_000929"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN2836896.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="ACG9BB_000930"
     CDS             complement(4877..5386)
                     /locus_tag="ACG9BB_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836897.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="ACG9BB_000931"
     CDS             complement(5561..7696)
                     /locus_tag="ACG9BB_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFN2836898.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="ACG9BB_000932"
     CDS             complement(7779..9434)
                     /locus_tag="ACG9BB_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN2836899.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="ACG9BB_000933"
     CDS             complement(9436..9750)
                     /locus_tag="ACG9BB_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN2836900.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="ACG9BB_000934"
     CDS             9920..10180
                     /locus_tag="ACG9BB_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFN2836901.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="ACG9BB_000935"
     CDS             10194..10508
                     /locus_tag="ACG9BB_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFN2836902.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="ACG9BB_000936"
     CDS             complement(11238..11792)
                     /locus_tag="ACG9BB_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFN2836903.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="ACG9BB_000937"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="ACG9BB_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="ACG9BB_000938"
     CDS             complement(14153..14869)
                     /locus_tag="ACG9BB_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFN2836904.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACG9BB_000939"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACG9BB_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFN2836905.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACG9BB_000940"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACG9BB_000940"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFN2836906.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="ACG9BB_000941"
     CDS             complement(18339..19799)
                     /locus_tag="ACG9BB_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2836907.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="ACG9BB_000942"
     CDS             complement(19789..21084)
                     /locus_tag="ACG9BB_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN2836908.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="ACG9BB_000943"
     CDS             complement(21416..22093)
                     /locus_tag="ACG9BB_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFN2836909.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="ACG9BB_000944"
     CDS             complement(22123..23214)
                     /locus_tag="ACG9BB_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFN2836910.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="ACG9BB_000945"
     CDS             complement(23315..23749)
                     /locus_tag="ACG9BB_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN2836911.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /locus_tag="ACG9BB_000946"
     CDS             complement(24333..25790)
                     /locus_tag="ACG9BB_000946"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN2836912.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="ACG9BB_000947"
     CDS             complement(25787..27682)
                     /locus_tag="ACG9BB_000947"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN2836913.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="ACG9BB_000948"
     CDS             complement(27740..29515)
                     /locus_tag="ACG9BB_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFN2836914.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="ACG9BB_000949"
     CDS             complement(29565..31061)
                     /locus_tag="ACG9BB_000949"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN2836915.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="ACG9BB_000950"
     CDS             complement(31255..32877)
                     /locus_tag="ACG9BB_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN2836916.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..>34469)
                     /locus_tag="ACG9BB_000951"
     CDS             complement(33205..>34469)
                     /locus_tag="ACG9BB_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFN2836917.1"
                     /translation="VKREVLAGFTTFISMAYILFVNPTVLGASGMDEGAVFTATALAS
                     AIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWETALAGVFVASLIFILITI
                     FKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSNESTLVGLGSLNVGSTWL
                     TIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPSEIVAAAPSLKPTFLVAL
                     KHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMKDNKMPRVGKALASDSTA
                     MLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLLGLFFSPLLSVVTSQVTA
                     PALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSISDGIALGFIFYPITMLA
                     AKRGKEVSPIMYGLFFVFVGFMWILNVK"
BASE COUNT         9777 a         7458 c         5827 g        11407 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccg
//
LOCUS       DAZAFF010000020        33678 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000020 DAZAFF010000000
VERSION     DAZAFF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.020"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            214..498
                     /locus_tag="ACG9BB_000952"
     CDS             214..498
                     /locus_tag="ACG9BB_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFN2836918.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(815..1198)
                     /locus_tag="ACG9BB_000953"
     CDS             complement(815..1198)
                     /locus_tag="ACG9BB_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFN2836919.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(1200..1784)
                     /locus_tag="ACG9BB_000954"
     CDS             complement(1200..1784)
                     /locus_tag="ACG9BB_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFN2836920.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="ACG9BB_000955"
     CDS             complement(1831..3306)
                     /locus_tag="ACG9BB_000955"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFN2836921.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="ACG9BB_000956"
     CDS             3487..3798
                     /locus_tag="ACG9BB_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836922.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="ACG9BB_000957"
     CDS             complement(3913..6249)
                     /locus_tag="ACG9BB_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFN2836923.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="ACG9BB_000958"
     CDS             6391..7059
                     /locus_tag="ACG9BB_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN2836924.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="ACG9BB_000959"
     CDS             7062..8105
                     /locus_tag="ACG9BB_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN2836925.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="ACG9BB_000960"
     CDS             complement(8342..9223)
                     /locus_tag="ACG9BB_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN2836926.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="ACG9BB_000961"
     CDS             9382..10110
                     /locus_tag="ACG9BB_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFN2836927.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="ACG9BB_000962"
     CDS             10266..10583
                     /locus_tag="ACG9BB_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN2836928.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="ACG9BB_000963"
     CDS             10576..11184
                     /locus_tag="ACG9BB_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFN2836929.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="ACG9BB_000964"
     CDS             11239..12030
                     /locus_tag="ACG9BB_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFN2836930.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="ACG9BB_000965"
     CDS             12168..12656
                     /locus_tag="ACG9BB_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFN2836931.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="ACG9BB_000966"
     CDS             12707..13579
                     /locus_tag="ACG9BB_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFN2836932.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="ACG9BB_000967"
     CDS             13755..14117
                     /locus_tag="ACG9BB_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN2836933.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="ACG9BB_000968"
     CDS             14126..14878
                     /locus_tag="ACG9BB_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2836934.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="ACG9BB_000969"
     CDS             14892..16694
                     /locus_tag="ACG9BB_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2836935.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="ACG9BB_000970"
     CDS             16971..18011
                     /locus_tag="ACG9BB_000970"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN2836936.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="ACG9BB_000971"
     CDS             complement(18063..20207)
                     /locus_tag="ACG9BB_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN2836937.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="ACG9BB_000972"
     CDS             complement(20564..21937)
                     /locus_tag="ACG9BB_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN2836938.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="ACG9BB_000973"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="ACG9BB_000973"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFN2836939.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="ACG9BB_000974"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="ACG9BB_000974"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFN2836940.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="ACG9BB_000975"
     CDS             complement(23385..24398)
                     /locus_tag="ACG9BB_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFN2836941.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="ACG9BB_000976"
     CDS             complement(24479..25078)
                     /locus_tag="ACG9BB_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFN2836942.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="ACG9BB_000977"
     CDS             25264..25965
                     /locus_tag="ACG9BB_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2836943.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="ACG9BB_000978"
     CDS             25975..28686
                     /locus_tag="ACG9BB_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFN2836944.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29137..30765
                     /gene="treC"
                     /locus_tag="ACG9BB_000979"
     CDS             29137..30765
                     /gene="treC"
                     /locus_tag="ACG9BB_000979"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFN2836945.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30825..32006)
                     /locus_tag="ACG9BB_000980"
     CDS             complement(30825..32006)
                     /locus_tag="ACG9BB_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN2836946.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32258..>33678
                     /locus_tag="ACG9BB_000981"
     CDS             32258..>33678
                     /locus_tag="ACG9BB_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFN2836947.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
BASE COUNT        10663 a         6038 c         6851 g        10126 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttattt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaccttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attatttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggac cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat aaagataaat
    29041 tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat ataagttgtg
    29101 gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga caaagtcatt
    29161 taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg agatttaaaa
    29221 ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat ttggctaaac
    29281 cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta cacagccatc
    29341 gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc aaagaaatac
    29401 cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca tgaatggttc
    29461 aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag agaagagcca
    29521 acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga tacggggaaa
    29581 tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa ccaagcagta
    29641 agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa aggattccgt
    29701 ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa agaaaatgat
    29761 ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga attgaaccaa
    29821 gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc tacgacgatc
    29881 gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt ctttaatttc
    29941 catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc atttgatttt
    30001 gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg taacggctgg
    30061 aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc
    30121 gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt gaaccgtggg
    30181 acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta ttcatcgatt
    30241 tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga acaaggattc
    30301 acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc
    30361 atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg gttaaaagtt
    30421 ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga ttctattttg
    30481 tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc acaaggagat
    30541 tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca atttgaagga
    30601 cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc catcccagaa
    30661 ggattcacag aagccaatat tcttatcgat aattattccg atactgttct tcatcaaagt
    30721 ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa
    30781 cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt aaaataatct
    30841 tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt attgtagcgt
    30901 tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata gataggaata
    30961 ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac gattaagatc
    31021 gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa gtcaccgtgg
    31081 agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt agagtcttgg
    31141 aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg aatgatacat
    31201 aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag taagaaacaa
    31261 acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag aaatgaaccc
    31321 aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca
    31381 atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa agcgacaatt
    31441 ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt
    31501 tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt cacgtcgtca
    31561 atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg
    31621 agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt
    31681 tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa aataatcaca
    31741 taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt
    31801 tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt agcgataatg
    31861 attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat cagcgcaagt
    31921 aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg gatcaaacga
    31981 ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataac ttcattatag
    32041 acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc gtcaatcatt
    32101 catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt taaccatatg
    32161 ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac
    32221 attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga taggtatatt
    32281 ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga aagccgagga
    32341 cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt ttacaaaagc
    32401 aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg attcccacga
    32461 ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct ttgtttataa
    32521 tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag aaggcaatgt
    32581 ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag aaggacatga
    32641 tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac atgtatggct
    32701 agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga ttgaagctta
    32761 cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc
    32821 actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca
    32881 acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat cgattgctgg
    32941 tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac actttgttaa
    33001 agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta ttgccagaac
    33061 gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag gattgacgaa
    33121 tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt
    33181 gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca aaaaagaaaa
    33241 gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc tttcagattg
    33301 ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg atcctgtttt
    33361 tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag aatattatac
    33421 gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga tggcatttac
    33481 gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag aaatcttgac
    33541 ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata aggaaagtgg
    33601 cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact ccgaacattt
    33661 tcatttatat gcaggaaa
//
LOCUS       DAZAFF010000021        33341 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000021 DAZAFF010000000
VERSION     DAZAFF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.021"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG9BB_000982"
     CDS             <1..1152
                     /locus_tag="ACG9BB_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFN2836948.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG9BB_000983"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG9BB_000983"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFN2836949.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG9BB_000984"
     CDS             2728..3003
                     /locus_tag="ACG9BB_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFN2836950.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG9BB_000985"
     CDS             3009..3776
                     /locus_tag="ACG9BB_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFN2836951.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG9BB_000986"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG9BB_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFN2836952.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG9BB_000987"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG9BB_000987"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFN2836953.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG9BB_000988"
     CDS             7920..8222
                     /locus_tag="ACG9BB_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFN2836954.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG9BB_000989"
     CDS             8225..9388
                     /locus_tag="ACG9BB_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFN2836955.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG9BB_000990"
     CDS             9414..12842
                     /locus_tag="ACG9BB_000990"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFN2836956.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG9BB_000991"
     CDS             12863..13987
                     /locus_tag="ACG9BB_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFN2836957.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG9BB_000992"
     CDS             13968..14414
                     /locus_tag="ACG9BB_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN2836958.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG9BB_000993"
     CDS             14398..14736
                     /locus_tag="ACG9BB_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFN2836959.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG9BB_000994"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG9BB_000994"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFN2836960.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG9BB_000995"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG9BB_000995"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFN2836961.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG9BB_000996"
     CDS             15893..16936
                     /locus_tag="ACG9BB_000996"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFN2836962.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ACG9BB_000997"
     CDS             17095..17823
                     /locus_tag="ACG9BB_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFN2836963.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ACG9BB_000998"
     CDS             17939..18184
                     /locus_tag="ACG9BB_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN2836964.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ACG9BB_000999"
     CDS             18181..18399
                     /locus_tag="ACG9BB_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFN2836965.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ACG9BB_001000"
     CDS             18535..19074
                     /locus_tag="ACG9BB_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFN2836966.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ACG9BB_001001"
     CDS             19067..21340
                     /locus_tag="ACG9BB_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFN2836967.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ACG9BB_001002"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ACG9BB_001002"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFN2836968.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG9BB_001003"
     CDS             complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG9BB_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFN2836969.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="ACG9BB_001004"
     CDS             complement(23167..23436)
                     /locus_tag="ACG9BB_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836970.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23698..23826)
                     /locus_tag="ACG9BB_001005"
     CDS             complement(23698..23826)
                     /locus_tag="ACG9BB_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2836971.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23906..24361)
                     /locus_tag="ACG9BB_001006"
     CDS             complement(23906..24361)
                     /locus_tag="ACG9BB_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFN2836972.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="ACG9BB_001007"
     CDS             complement(24499..26427)
                     /locus_tag="ACG9BB_001007"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN2836973.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="ACG9BB_001008"
     CDS             complement(26624..27457)
                     /locus_tag="ACG9BB_001008"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFN2836974.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="ACG9BB_001009"
     CDS             complement(27649..28353)
                     /locus_tag="ACG9BB_001009"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN2836975.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="ACG9BB_001010"
     CDS             complement(28376..29002)
                     /locus_tag="ACG9BB_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFN2836976.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="ACG9BB_001011"
     CDS             29328..31703
                     /locus_tag="ACG9BB_001011"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFN2836977.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="ACG9BB_001012"
     CDS             complement(31727..32218)
                     /locus_tag="ACG9BB_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFN2836978.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="ACG9BB_001013"
     CDS             32405..33325
                     /locus_tag="ACG9BB_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN2836979.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
BASE COUNT        11021 a         5845 c         6925 g         9550 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt t
//
LOCUS       DAZAFF010000022        32556 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000022 DAZAFF010000000
VERSION     DAZAFF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.022"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACG9BB_001014"
     CDS             complement(42..230)
                     /gene="rpmB"
                     /locus_tag="ACG9BB_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFN2836980.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="ACG9BB_001015"
     CDS             500..862
                     /locus_tag="ACG9BB_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN2836981.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="ACG9BB_001016"
     CDS             914..2596
                     /locus_tag="ACG9BB_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFN2836982.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="ACG9BB_001017"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="ACG9BB_001017"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFN2836983.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="ACG9BB_001018"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="ACG9BB_001018"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFN2836984.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="ACG9BB_001019"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="ACG9BB_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFN2836985.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6431..7435
                     /locus_tag="ACG9BB_001020"
     CDS             6431..7435
                     /locus_tag="ACG9BB_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2836986.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7436..8380
                     /locus_tag="ACG9BB_001021"
     CDS             7436..8380
                     /locus_tag="ACG9BB_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2836987.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8380..9342
                     /gene="opp4B"
                     /locus_tag="ACG9BB_001022"
     CDS             8380..9342
                     /gene="opp4B"
                     /locus_tag="ACG9BB_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFN2836988.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9369..10283
                     /locus_tag="ACG9BB_001023"
     CDS             9369..10283
                     /locus_tag="ACG9BB_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2836989.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10309..12090
                     /locus_tag="ACG9BB_001024"
     CDS             10309..12090
                     /locus_tag="ACG9BB_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN2836990.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12438..13124
                     /gene="rnc"
                     /locus_tag="ACG9BB_001025"
     CDS             12438..13124
                     /gene="rnc"
                     /locus_tag="ACG9BB_001025"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFN2836991.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13144..16725
                     /gene="smc"
                     /locus_tag="ACG9BB_001026"
     CDS             13144..16725
                     /gene="smc"
                     /locus_tag="ACG9BB_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFN2836992.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16734..17543
                     /locus_tag="ACG9BB_001027"
     CDS             16734..17543
                     /locus_tag="ACG9BB_001027"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN2836993.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17554..18552
                     /gene="ftsY"
                     /locus_tag="ACG9BB_001028"
     CDS             17554..18552
                     /gene="ftsY"
                     /locus_tag="ACG9BB_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFN2836994.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18739..21006
                     /gene="gshAB"
                     /locus_tag="ACG9BB_001029"
     CDS             18739..21006
                     /gene="gshAB"
                     /locus_tag="ACG9BB_001029"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFN2836995.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21095..21547)
                     /locus_tag="ACG9BB_001030"
     CDS             complement(21095..21547)
                     /locus_tag="ACG9BB_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFN2836996.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21697..22647
                     /locus_tag="ACG9BB_001031"
     CDS             21697..22647
                     /locus_tag="ACG9BB_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN2836997.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22640..23599
                     /locus_tag="ACG9BB_001032"
     CDS             22640..23599
                     /locus_tag="ACG9BB_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN2836998.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23596..24351
                     /locus_tag="ACG9BB_001033"
     CDS             23596..24351
                     /locus_tag="ACG9BB_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2836999.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24374..25327
                     /locus_tag="ACG9BB_001034"
     CDS             24374..25327
                     /locus_tag="ACG9BB_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN2837000.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25971..26267)
                     /locus_tag="ACG9BB_001035"
     CDS             complement(25971..26267)
                     /locus_tag="ACG9BB_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFN2837001.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26622..26978
                     /locus_tag="ACG9BB_001036"
     CDS             26622..26978
                     /locus_tag="ACG9BB_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN2837002.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26988..27887
                     /locus_tag="ACG9BB_001037"
     CDS             26988..27887
                     /locus_tag="ACG9BB_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN2837003.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28407..29135
                     /locus_tag="ACG9BB_001038"
     CDS             28407..29135
                     /locus_tag="ACG9BB_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN2837004.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSRWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29280..31307
                     /locus_tag="ACG9BB_001039"
     CDS             29280..31307
                     /locus_tag="ACG9BB_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFN2837005.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31415..32512
                     /locus_tag="ACG9BB_001040"
     CDS             31415..32512
                     /locus_tag="ACG9BB_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFN2837006.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT        10861 a         5667 c         7002 g         9026 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaaggatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcgat
     6301 tttctatata attattaata attcattaca caaagataag aataaccttt gttataaaac
     6361 gctttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag
     6421 gggtattcga ttgtccgata accaattatt agaagttcag aacctacata caggattccg
     6481 actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga
     6541 gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat
     6601 gggattactt gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa
     6661 tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat
     6721 tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga
     6781 aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt
     6841 gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact
     6901 atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat
     6961 cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga
     7021 tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt
     7081 aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga
     7141 agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact
     7201 gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt
     7261 tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat
     7321 accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt
     7381 acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac
     7441 agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa
     7501 tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa
     7561 gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt
     7621 gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg
     7681 aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat
     7741 gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt
     7801 tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg
     7861 gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg
     7921 tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt
     7981 atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca
     8041 agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg
     8101 cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat
     8161 ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga
     8221 tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca
     8281 aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac
     8341 tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga
     8401 cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc
     8461 caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg
     8521 atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt
     8581 tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt
     8641 gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc
     8701 cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc
     8761 ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc
     8821 ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca
     8881 caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt
     8941 ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga
     9001 agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta
     9061 ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct
     9121 caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt
     9181 ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg
     9241 atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat
     9301 atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga
     9361 atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga
     9421 tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc
     9481 tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga
     9541 tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa
     9601 agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg
     9661 ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg
     9721 gattagggat catctctggc tactacggag ggatcattga caacatcttg atgcggatcg
     9781 ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt
     9841 ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag
     9901 cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa
     9961 aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt
    10021 cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt
    10081 tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt
    10141 atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc
    10201 ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag
    10261 atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg
    10321 atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt
    10381 acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt
    10441 ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc
    10501 gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat
    10561 gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc
    10621 acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa
    10681 gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc
    10741 ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt
    10801 aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa
    10861 aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa
    10921 tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc
    10981 aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac
    11041 atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat
    11101 ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa
    11161 gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc
    11221 tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta
    11281 ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca
    11341 aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta
    11401 ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc
    11461 cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa
    11521 ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat
    11581 ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg
    11641 gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt
    11701 cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat
    11761 gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca
    11821 aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat
    11881 atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa
    11941 caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca
    12001 gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat
    12061 gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt
    12121 cctttttcgg ttttttgata tttttctgtt tatttctcta atattcaggt caaggcgctg
    12181 atttcatgac ttctccaata aacggtgttc aaagtccagt ttctcggtat taagtcaaat
    12241 ttatgcaaaa atatggaaag ccattttcac aaaatttttt cttcatactc gaaactaaac
    12301 gaatttttca caacttctcg tcaatcacta ggattattaa agctttactt accttttgac
    12361 cgaaccattt caaatttgac aaaaaaccag tataataagg aagtatcgaa aaaatatcca
    12421 taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa aactataaca
    12481 tcgtttttca cgatctaaat ttgcttgaac aagcttttac ccattcatcc tatgtgaatg
    12541 agcatcgcaa tctgcaatta tcggataatg agcgacttga atttttagga gatgccgttt
    12601 tagaactgat ggtttctcag tatttattcc gtttatatcc tcatttgcca gaaggaaaat
    12661 tgacaaaaac acgagctgcg atcgttcgcg aagacagctt gtcgaaattt gccaaagaat
    12721 gtcactttga tcaatatgtt ttgttgggca agggagaaga gaattcagga ggacgtacgc
    12781 gaccagcatt gctttgcgac ctattcgaag cttttttagg cgcgttatat cttgaccagg
    12841 gattcgacac gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg
    12901 ctttttcaca tgagatggat cataaaacaa aactacaaga agtcttgcaa aaatcaggtg
    12961 atgtatcaat cgaataccga ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt
    13021 ggatcgaagt ttatgtagat gatcaattga ttggcactgg acaagggaaa tcgaaaaaat
    13081 tagcagaaca agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa
    13141 gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat catttgcaga caagacagtc
    13201 attgattttg aaaatagcgt cacagcagtg gttggaccaa atggcagcgg aaaaagcaat
    13261 attacagagg ctatccgctg ggtattagga gaacaatctg ctaaaagtct gcgaggcgga
    13321 aaaatgccgg atatcatctt tgccggttcc gatacccgta agcagctgaa tattgcagaa
    13381 gtcacagtga ttttagacaa tagtgatcat tatctgccat tggagtacaa tgagatcagt
    13441 gtaacccgcc gttatcgacg gactggggaa agcgagtttt tcatcaataa acaaagctgc
    13501 agattaaaag atatccaaga attgttctta gattctggat taggaaaaga atcgttctca
    13561 atcatttctc aaggaaaagt agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg
    13621 atctttgaag aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag
    13681 aagctttttg aaacagaaga caacctcagc cgcgtccaag acatcatcca cgaattggaa
    13741 gaacagttaa ctcctttggc cgcccagagt gaggcagcaa aggagttttt acggctaaaa
    13801 gaaacactga ctcaaaccga tgtctctttg atggttgctg aaatcaagac agcaaaaaaa
    13861 gattgggaca acaaacaagc acagcttgcg aaattcaatc tagaattagg caaactatct
    13921 gagtcgatcc aagaacaaga atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc
    13981 gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa actgaagcag
    14041 acagaaggac aaaaagatgt tttgcaggag cggacaaagc atacacaaaa aagctcgcaa
    14101 gaatatcaaa cttcattagc agaagcacaa aaaaaggtta aacattttga aaagcttcaa
    14161 gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat
    14221 ctcataaaaa cacagcaaga attagaaaaa taccaaaaat caacgaaaga attattggca
    14281 gaattaagag atcaatatgt tgatttgatg caagaacaag cagctgtagg aaacgaactg
    14341 aagtatctag aacgtcaata tatacaagaa acagcaaaaa gcaagcagac gttagccaag
    14401 caatcggaag tagaagcatc agtagaccga ttgatcttac aaaaagaaga attgacacaa
    14461 aagcaggcgc agctaaaatc atctttaact gaaacaaaag agaagttaga aatgatccaa
    14521 caaaacggga aaaagttcca agaagcatta gcaaaagaac agcctaaaat gtatcaatta
    14581 atgaatcaag tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac
    14641 tattttggtt tttaccaagg ggttcgattg gtcttgcagc ataaacagca gctttcgggt
    14701 attgtcggag cagtggctga actgatcgat gttcctgcag atttcacgtt agctatcgaa
    14761 gcagctttag gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa
    14821 gccatcacgt atttgaaaca gcaaagagga ggaagagcaa cattccttcc actgacgaca
    14881 atcaaaccaa gacaattacc ggctcatata ttgacacaag cagcagctgt agaagggttt
    14941 atagggattg ccagtgaaca agtcagttat cccgatcaaa tccaaactgt ggttcaaaac
    15001 ctgctaggaa cgatcctttt agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg
    15061 atccgctatc aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc
    15121 atgacaggtg gagcgaataa acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa
    15181 ttgaaacagt tgactagtga atttgaagaa gcagataaac aactccaagc acaagaaaaa
    15241 aaagttcagg agctgcaaca agaaacagct cgtttagccg aagaacaaga agttttgcgt
    15301 acacgaggcg aacaattacg ttttgaagaa caagaagcga caaatcagct acaaaacatc
    15361 ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct caacttttga gacacgtgaa
    15421 ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga caaacaaaca
    15481 gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc
    15541 gaccagatgg aagccagaag agcacaagta caaagtcaaa aagcacaaga acaagcagat
    15601 cttgctgtct tgaaagagca gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc
    15661 caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa agcagttggc gacattgaca
    15721 gcagactttt ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat taacgaatta
    15781 tctgctcagc gagaaacatt aaaagccgag ttggcaaaag ccaaagagca gcgagataga
    15841 aggcaaaagg agatcgataa gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa
    15901 gcacgactgt cggaacaatc caaattagaa gtccaaaaag accgagctga aatgatttta
    15961 gataatcatt tgacctatct gcagtcagaa tatcagctga catttgagaa agcttcccaa
    16021 gactatcaag aaacaaccga tatcgaagac agtagaacaa aggtttcatc cttaaaagaa
    16081 cagattgaaa aactgggacc agtaaactta aattcaatcg aacaatacga acaagtcagt
    16141 gaaagacata cattcttagc aacacagcga gatgatctgt tagcagcgaa aaaccaattg
    16201 tttgaaacga tggatgagat ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa
    16261 gctatccgtc aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa
    16321 ttagtactga cggaaccaac tgatttgctg aaaacaggta ttgagattga agtacaacct
    16381 cctggcaaaa aactgcaaag tctaagtttg ctttctggag gagaacgagc acttacagcc
    16441 atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc cgttttgtat cttagatgag
    16501 gtagaagcag cgttagatga agccaacgtg aaacgttttg gtcgttactt gagcgatttc
    16561 caagatgaca cacaatttat cgtagtcaca caccgcaaag gaacgatggt cgcagcagat
    16621 gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg
    16681 gaagatatca atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca
    16741 aattagtagc aatcgatttg gacggtacat tattaaatga acaaaagctg atttcagatg
    16801 agaataaaca agcattagca caagcaaaac agcaaggggt caagatcgtt ctatgtacag
    16861 gacgcccctt agctgcgatg gcgcattatc tgcaagaatt aggactggta gatgaaggtg
    16921 actacagcat cactttcaac ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg
    16981 aaaagaaagt gatggaagta tcagatatcc aacgcttata caaactggct cagcaattgg
    17041 atctgccact agatgttttg tctgacaatg tggttttaca gctgcctagt gccccaagga
    17101 agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg gatataagcg
    17161 atattacaga tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa gaagaattag
    17221 atcctaaaat caaagaaata ccagcagaat tccatgaaat gtatgagatc atcaaaacaa
    17281 gaagtatgct tttggaattt atgccaaaag gtgttacaaa agcttatggt atttctttat
    17341 tggcaaaaga tcttggtttg aaacaagaag aaatcatggc gatcggtgat gaggaaaatg
    17401 atttaccgat gatccaatat gctggactag gcgtagcgat ggagaatgct gtgcctttcg
    17461 tcaaagaagc agcagatttc gtgacagcca gcaatgaaga aaatggcgta gccaaagtga
    17521 tccaaaaatt tgtattagag tagggggagt caaatgggat tttttgataa aatcaagcgt
    17581 gcttttactg gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt
    17641 ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga
    17701 acagtcgatg aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt
    17761 tttgaaacag cgatacgaat caccgaagca ttacgtcaag aagtgaaact cagaaatgcg
    17821 aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg
    17881 gaaggaatca atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg
    17941 tttgtcggtg ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc
    18001 aaaaacgaag gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc
    18061 gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga
    18121 ggcgatccag cagctgtcgt attcgatgct ttggaaagag caaagacaga gcaggcagat
    18181 gttttactag ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta
    18241 gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt
    18301 gtattagatg caaccactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca
    18361 acagatgtga ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta
    18421 cttgctatcc gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc
    18481 gatgacttgg agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta
    18541 aaagaagaat aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt
    18601 tgaatgattg tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta
    18661 agcggttctc aaacaatttc gttctttttc ttagactttt tgattataat tagattcata
    18721 tgaattggag gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc
    18781 ccttttatcg accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca
    18841 ggaaatctag caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat
    18901 atccaaacag attttagcga aacacaaaca gagatgatca cccctgttac cgattctatt
    18961 cccgaattat ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa
    19021 gaagagatga tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagagatc
    19081 attattgcaa aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaaaaa
    19141 tacgggaagc gaaaacagat ggtaagcggt attcatttca attttgaatt tggcgatgag
    19201 ttgctaagaa cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca
    19261 gaactttatt tgaaaacagc gagaaacttt atgcgctatc gctggatgat cacttattta
    19321 ttcggtgctt caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa
    19381 cctgttcgca gtattcgcaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt
    19441 tcctatgctt ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa
    19501 ctttcggagg agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca
    19561 gatttagcaa caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat
    19621 gcaagattgg gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg
    19681 ttatggacag aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat
    19741 aacaaagtag cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa
    19801 attcttgaag gaatgaagca aatgctggtt gaattggatt ggttggattc tctttattta
    19861 gtagaggaag cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat
    19921 caagaagcac agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat
    19981 aaagaaagcc atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact
    20041 cagatattta tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg
    20101 gatcaatttt tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg
    20161 acaagtaagg acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa
    20221 gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa
    20281 gcggtaaaag cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc
    20341 aattacgggt taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca
    20401 ggtttagcca ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga
    20461 acggaatatc gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct
    20521 gctaatgtta ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt
    20581 gaccctttgc gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta
    20641 gaacaattga tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa
    20701 attgtttatc taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact
    20761 gatgaatttt ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct
    20821 aagattagtg gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa
    20881 aaagcatatg ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatcct
    20941 tttgcaggaa aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta
    21001 ttttaaaaga aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt
    21061 tttttgcatg taaaaattta tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt
    21121 ttttcttttt tggtttttct ttttcagctg gcgaagagtt ttcttctggg tatttttttt
    21181 cgatatcaat cacaggttca tttactgctt cttttgctgt taagagcaat ccgaattcct
    21241 caggatttgc agtgacgtaa tcgttgacta cagtgaaacg gctattgttt ttcgttgcta
    21301 gctggatata ttggttttgg acagaaaggg gtagagcccc attcaagagt atggaagcat
    21361 ctggatgttt tttgagttct tttaaaaagt tttctttgtt ttttggttgg atcatttcag
    21421 caacagtcat actaagatag cagcgttccc tgaatgtccc taaatattta tgctgttctt
    21481 cagggttcac taaaggtgta ccataaagtc ccttatccaa atggcttttt acatcatctt
    21541 gtgccataaa aaataccctc cttcttctct ttgtctttct tacgattata gcatattccc
    21601 aataatacct aaaacatatg aaaaagcggt tgcttttgtt caacgattca cttaaaataa
    21661 gaatgaaaat cattctcaat tagaaaggtg gattggatgc gctacggacg actagtagca
    21721 ttattagtta ttttaagtag tatttcattg ttcatcggag tatcgaatgt acaattgtca
    21781 gaactgctcc agttagatgg gcaaagctta caaatcttgc tagtgactcg tttgccacga
    21841 acgatttgtt tgatccttgt gggagcgacc agtagtattt gtgggttgat catgcaacat
    21901 ttgacccaaa ataaatttgt ttcaccaacg actgcgggca cgatggatag tgcgcgttta
    21961 ggtattttag tggctatgat tttcctacct ggtgcatcct tgattgtccg atccttgaca
    22021 gcattttgct ttgcttttgc aggaacattg ctttttttat cccttacgcg attgttcctc
    22081 cagaaaaatc aattgatgct tccgttgatt ggagtcatgt tagggaatat cattggttca
    22141 gttgctactt tttttgctta tcaattccag ttggtacaaa atatgacttc atggcttcaa
    22201 gggaattttg cgactgtgat acgaggaaat tatgagttat tgtatgctgt tgttccattg
    22261 ttgctcttgg tttggctatt tgcttaccga tttacgatca ttggtctggg cgaatcgttt
    22321 gcagtaaatc tgggaataga ttaccagaag atgcagtttt taggcattgg cttagtatca
    22381 ttggctagtg cagtcatgtt gatcatggta ggaagcattc cttttatcgg agtgatcatt
    22441 ccgaatcttg tctctagaat atatggagat caagtgcaca aaacaattgg cgtaactgct
    22501 ttagcaggta gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct
    22561 tatgaaatac cagtcagtgt gatcgttgga atcatcggtg gggctatctt cctttatctt
    22621 ctgataaggg gacgacgata tgcttaaaaa aaagccatct atttttattg gtatgcttgt
    22681 tcttgtttta tttgttacag gaatgtactt gacgatccat acatatggaa actggcattt
    22741 tgcatgtgca ttgaggagta agaaaatttt tgcatttatc cttgtctcgc tagctgtcag
    22801 cagtgccaca atcagctttc agacaattac gcataaccag tttttgactc cttcagtatt
    22861 gggacttgag tcgttatatg tccttgtcca aacggtcctt ttcttcttta tcggaggagt
    22921 gacgatgctt cagcagacag gtacagtttt tttcttattg aacgttgggc tgatggtcgg
    22981 gctgagtcta ttgttatttt tctttctttt gaaaaaaatg agagcgaatc tttttttatt
    23041 actgatgtca gggatgattt taggtacgct ttttggtagt gtcagtacct ttttacaagt
    23101 catcatggat ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa gctttggcaa
    23161 tatccagagt cagtatcttt tgcctgcttc tttgatcatt atcggctgct ggtacctgtt
    23221 atttcgatat cgacatgtgc tagacgtttt ccatctcgga aatgaacaag cagtaaacct
    23281 cgggatagac acagatcgtt ttgaattttt gattttatgt tgtgtcagtc ttttgacagg
    23341 gacatcaacg gctcttgtgg gtccagttac ttttttaggg tttattgtcg caaatatcag
    23401 ttatcgtgtc ttttctactt atcgtcatgg tcagctattt atcggaagct ttctggtagc
    23461 tatgctgttt ctgacaggag gacagctcgc agtagagcag attttcggct ggaatacaac
    23521 agtgagtgtc gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat tgattcgcga
    23581 aaggaagagt atcctatgat tgatttacaa aaggtcaata aactgtacca tggaaaatca
    23641 gtggttaaag atgtacagtt aacgattcct gaaaagaagt tgactgcttt tatcggtcca
    23701 aatggtgctg gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc aaaagataca
    23761 ggtgaaatct atttagacca taacgaagta aaggcttgga aatccaatga attagccaaa
    23821 aaattatcga tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta
    23881 gtagaatttg gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc
    23941 gttcaggaat cactggaaca ccttgggtta gaagaaatag cggatcaatc gattgacact
    24001 ctttcaggcg gtcaactcca acgtgcgtat attgcaatgg tgctagcgca agacacggat
    24061 tatattttac tggatgaacc tttgaacaat ctagatatga attttgcggt tcaaatcatg
    24121 cagattttaa aaaatttagt acaagaaatc gggaaaacaa ttgttattgt tcttcatgat
    24181 atcaactttg ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg acagttattt
    24241 acgcaaggaa cgacccaaga aatgataaaa aaagatgtat tggatgaatt atacgaaatg
    24301 gacatacgga tttgtgagat tgaaggccgc agattctgtc tttatttcta aaactaacat
    24361 taggagagaa gagatgaaaa aattagtagt aggtatcttg attctagcat catttggttt
    24421 agcagcatgt ggaaatagca acaacaccag tcaggcagac acaaaaaaaa gcagcacaca
    24481 aactgaaact acattgacga taacagatag taatggagac caaatcgagg tgccaaataa
    24541 tccagaaaaa gtcgtcgtat tcgacaatgg ttctttagat acgatggatg ctcttggtgt
    24601 tggagataag gtagttggag cagctacaag cagccttcct gagtatcttt catcctataa
    24661 aaaagtagaa tcagcaggtg gaataaaaga accagactta gaaaaaatta atcagcttca
    24721 gccagatcta atcatcattt ctggccgaca aagagatttt caaaaagatc taagtcaaat
    24781 tgctccgacc attttccttt ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa
    24841 tgtcactgct ttaggggaga tctttggtaa gcaagaagaa gctaaaactc aattagagga
    24901 attatctagt gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca aaaaagcact
    24961 agtcacactt gtgaatgaag ggcaattatc agcctacgga tctggttcta gattcggttt
    25021 catccatgat ttatttggct ttgaacaagc agatgatcaa atcgaagcat ccactcacgg
    25081 tcaaagtgtt tcttatgaat atgtgctgga aaaaaatccg gatatcttat ttgtagtaga
    25141 cagaacaaaa gctattggtg gagacgacag caaagatgat atttctgcca atgaactggt
    25201 agcacaaacg aatgctggta aaaatcagca gatcatctcc cttgaaccag atgtctggta
    25261 tttaagcggg ggcggtttag aatcaatgaa actgatgatc gaagatgtga atcaagcatt
    25321 taaatagcat aaataacaga ggattaaact ctcatactat gtaacaataa tgtttcttac
    25381 attcttaaat agcaagagcc tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt
    25441 attgatgatt ttacggatgg tttttattta tttgagaaat cttttttcaa cgtgaattca
    25501 ttatctacat catacgggtt gattctagaa tggggagtgg caaaaaatag tgtgatccat
    25561 tttttcagca tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga
    25621 taaaacttaa atttagatat ttttattttg taccgatctc ttctttaaac gatgttccat
    25681 ctgctgcttc tcggcaataa gtccactaga ctagaaaata tgagaagcta ttttctgtcc
    25741 agcgttctta ttactcaaaa caaatcgcag ttgtcacaat ctcttctaaa cgatgttcca
    25801 tctgctgctt ctcggcaata agcccactag actagaaaat atgagaagct attttctgtc
    25861 cagcgttctt attactcaaa gcaaatcgca gttgtcacaa tctcttctaa acaatgttca
    25921 atcagctgac cttcggtatt agctcaaagc actgatttca caacctctat ttaggcatgt
    25981 gtctcagcaa gcggttgata gttgatgatc tgcatttttc gataaaaatc ggcgggaagc
    26041 ttactccacc gttttagtaa ttgatagaaa taactctctt ctgctttgat tttatactcg
    26101 cttaatatca atccaaaact attcgttttt tttgaagcta attttttata aatatccaat
    26161 gctttggttt tatcttcatt gacaaagagt gtttttactt caatataaga accctcgata
    26221 ttttgtgttt cttcgataaa atagatgttt tttatatatt ccttcatagt gattcctcca
    26281 aagaagtctt ttatttgata agtttactct accgaagagt tgttaataat ccgtgaacaa
    26341 aaagtaattc tttttccaat agttgttaaa ggttttttat tttttatagt attttttgtt
    26401 atttaagaaa ggaataaggt taaacaaata acaggttgct ttttgggtct ttttccgttc
    26461 aagaaactga acgaagaaaa gggaaagttg tctcttttgt gataagagaa atcataataa
    26521 acgaatgact tactttccaa taaattgaga acagtcagaa ccagacaaat tagttgcatc
    26581 tgatgaataa agtgattatt ctatgacatg aggtgataca gatgaaatta gaagtgacaa
    26641 aagaagcaca agaagtattg aagaataaat tagaagcaga cgatcaactt ttattagata
    26701 tcgaaaacgg tgacggtcca tttgcagaaa gccaagtcag ttgccaattg gatacggcat
    26761 ttcgattgat cattgtcaaa aaagatacac aaacagatct aagtctttat tctgtagtgg
    26821 ctgatacacc agttggacca atcaaaatca aagacagtgc aatactttac atggatgatc
    26881 catccacact tgctttagaa ccaacttata atagtttaca attaaaagga ccaagcggct
    26941 tgagaaaagg caacttgcaa gttgttcgaa aagactagag ggataaaatg ggaaaattgg
    27001 cgatcatcag cgacctacat gtggacatca ataaattcgg cgaatcagag ctgatgcagc
    27061 tttggcaagt tttgcaaaag cacaagatca ctcggcttca tctagccgga gatacggcta
    27121 ataaagtaga ccgttgccta gctgttgtcg aatttttcgt caataaagga ttatctacga
    27181 catttcattt cggcaatcac gagttggcag atgtgcccaa tgaacagatg atggaccatt
    27241 ttccagaccc acatttttta aatgaacggt atattgcttt aaatgagcag accgttcttt
    27301 tagggatgaa tggctggtac gactatcagt tttccgaatt gaaagattac gaggcaatcg
    27361 tacgaatgaa gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa acagatctcg
    27421 aggtcaatgc agaaatgctt gcagctgcaa aaaatttgct agaccagctg gaagcaaaaa
    27481 aacttgaggt gattttatcc actcatttcg ttcctaaacg ggcatttatc atgtatcaat
    27541 ctgctcctta tgagcgttgg aacaaattaa atgcattttt aggatcagaa tcattcggag
    27601 aattacttga ccgttattca aatatcaaac aagtagtttt tggccatacg catcgtcgct
    27661 ttgaagataa atccatcggt cacacgattt acagctgcag accttttggt tattattatg
    27721 aatggcagct gacccgagat tttgtcttga aagaacagtt gctagacagc tatgatccta
    27781 tgaaattacg tgtcctcttg cgtgcccatc acccagcttt tcaggcctat caaacacaac
    27841 atttgcaaga ggaatttgaa caagcgatga caatcgttcc gtattgagac agcattagta
    27901 atgtgtaaaa gaaaatacct tgtaaaacat aaggataagt aagagcagta gatccattta
    27961 tcgtggatat gctgcttttt ttgcggaaaa taatcagcga atgtttattt gatcggatga
    28021 tttgtaactc gcagagtact agatgcaagc gtgtattctt tttttgaacg ataatcacaa
    28081 aaaaaaatat aatatacatt ttttatttaa ttataattaa tattgattga agtctaggca
    28141 gtcaagtcac attttgatag ataggagggg gacttacgaa ttattttaaa ggctatgaca
    28201 gctgtatttc tagattgtgt ttgcatcaag ttgcaataaa aactagaaat caaatcgggc
    28261 tgataatcat ttataactaa tcgatacata ggaccaaata taggtctatg ttcttttatt
    28321 tcataagtat cgtaaaacag aattttgcta tggattaatc caatcgactg atatgtttag
    28381 taaataaaga aagaggaatg tcaatgatga aaaaagaaat cgtattaaca gcagcaatga
    28441 tgttattcag catggtagca agtacgactt ttgtttctgc aactgaagta tacccaaagg
    28501 aatataacac agaaggaacg attacatttg aagcaggaga tgagggtgta actcctcctg
    28561 ttgatccaga gaatcctgat cccaataaac ctgtcgatcc atcagatcct ccaagccctg
    28621 gaacaggagg ggcattatcg attgattatg gatcaaaatt caaatttggg actcaaaaaa
    28681 tttctactgc ggataaaacg tattatgcag ctgctgatgt gatgaacgat gggtctagaa
    28741 aaccgacata tgtccaagtg acagatagga gaagtacctt atctaggtgg aaattaagtg
    28801 tctctcaacc agaacagttt aaaactgcca gtggggatga actagttgga gcacagctga
    28861 aatttacgaa agggcaggct gtttctttgg ttgatccaac atatacgccg caaacggtga
    28921 attctgaatt aacgttgaca ccgggaggaa acaatacgtt agcaattaat gctaaatcag
    28981 gaacaggtgt ggggacttgg gtctatcgat ttggtgcaaa tgaaaatgaa aatcaagatg
    29041 cggtacagct ttcggtaccc ggtaaatctg tcaagttagc gcaacaatat tccactaaat
    29101 tagtttggac attagaagat acacctaaca actagtttac tgaaaagaac tgttaaaaat
    29161 aaatacgtag tcatattgtc ttgcgaggta atcgagcaaa aattgtcaaa ggctaggcta
    29221 aatttgtttg gatacgaata gttcatctag gaatttaacg atcaggagga aaagatatat
    29281 tgaaaaagag gaggatcgtg ctcattttaa gcagtctctt agtgactaat atctttccga
    29341 tcatagggta ctctttggaa ttgaatcagt tggagcatgg aaatagaaga gacgaagaaa
    29401 gcaaactcac ttcgacattc gacagtttaa tagaatccaa ccagacacaa gaaagcagtg
    29461 aggatttaag tatttcgagt agtggagaga ccactgacaa cgtagaagct gagggttcgg
    29521 acaaacaagg actagaagat gcaccaattt ctgcggttga gctgcctgac gatcccaata
    29581 ttatttctat cgataaaatt tttcaaacac ctatcggtac tagcacgtcc atattggaaa
    29641 atgggaagtt actgcagttg aacccagcag aaaggtctca aagaggagct atttggtcaa
    29701 ataagaagat tagtttactc tcggatttta catttaaatc ttatttatac ttaggaaatc
    29761 aacgagatag agcgggagat ggcatgacct ttacattgac tggcgatcct agaatggcta
    29821 gtaatcccag tcaagttatt ggatcgccag gtatgggaat tggctcatat tctactcgtt
    29881 ctggccaacc ttacattgcg aatgcgctct cgattgagtt tgatacgtat aaaaatagtg
    29941 gttcttctga tcgaatggat cgagagataa ctgctgataa tagagggcat ggacatgtcg
    30001 cttttgtcac gcctaaacca aataataata attattctgg agagcatact ggcgtgacgg
    30061 tagctcctac atttctttct aatggcacat ggcgcttgtt aataattaaa tgggacgcgg
    30121 agcaaagaag tcttacttat gatttggaag gtgtcggtcg ctcttctcgg attatacctg
    30181 atctcattag gcagtttggt tctacagaag taaattgggg gtttacttct tcaacaggta
    30241 ctttttttca agaaaatgct gtcgctgtca cgcgacttcc atcgaatgtc acatcaacag
    30301 ctgatgtaaa agtcaacgat agtgattatg gggccatcgc tgaagctgga ggaaacgata
    30361 agataactat cagaaataca ttgaaggtcg agggggattt tcttgaatat aagaaaatgc
    30421 agactagtat cgacctccca caagaattaa ctattcccga gcatatcttg atcaatggaa
    30481 atcaaattga tgccaaagat atcttgataa ccggaaatcg agtcacgcta aatctgacag
    30541 attattttat tgctggagat acgttaataa tcgaattgga gacaactttg caggaagtaa
    30601 ttcctgaaaa agtactcact atgcattttg aataccttga aaacgggaat ttgattgagc
    30661 aatcgaatga aataaaccta aagatcgcaa agctgaagga gatagatatc catgtttatt
    30721 ataaagataa agacagtcag attgatattg ctgaaatgaa aacaatcact gggaaaatag
    30781 gagagaagta taaagaaact cctattgata ttgaagggta tgtttttgac tcggattcag
    30841 cgaatacaga aggcatcttc tccgaaaata cagaggatat ctatttttat tacagaaaag
    30901 gcaaacttta tctctcaact gctcctcaat atgtcaattt tggaaaacat aaaatatcca
    30961 gtagaccatt aagtgaattc ggacagtttt caggtgggtt gaaaattata gatgaacggg
    31021 cttttggaag ttggaaatta caattgaaac aagagaaagt tcttacaaat ggagattttg
    31081 agcttccaga agctctttcc tttgttacct ctgaaaatac aacggttata gggacatccg
    31141 ctgttacgat tttcgaaagt gatcaaaagg gtgaaagcga tttgtcctct cttttagatt
    31201 ctacagctca tcgtggaatc agaataaata ttcctgtaga aaaccaaaga atcggaacct
    31261 ttgaggggaa actatcttgg atacttgcag atgtcccagg aaattaacag gaagagaaga
    31321 atataacaaa attattgagt ttcaagaagg gcacaggtac cataggctct tgtgtcccac
    31381 agagacaaga ttttgttata gtcttctctt acatatgatt aaaggagcga ttttcttgag
    31441 accaaaacaa caattattta taacactcct agttatttta ggggcacttt ttaacaatat
    31501 atcagtgtca gctagtgcat ttaatttttc agtgacgccg ataacttcag agaatcaaat
    31561 cgacaagaga aaaacttact tcgatcttca acttgatccg gatcaggaag tagaagtaaa
    31621 agcggaattg agaaatgata cagaaaaaga agtgaaaatc gatatttctg tgaatagcgc
    31681 aaccaccaat tcaaatgtga tggttgaata cggaaaaaac gagattgaaa aagacatgag
    31741 cctgatcttt gatctggtag attatgtgag ctatccacag atagtgacgc taaaacctaa
    31801 aagtgtccaa accgttgctt ttcatgtcag gatgccgaat gagcgatttg atggtgtatt
    31861 ggctggtggg atcacattcc aagaaatcca gacggaaaag gaccaaacag aaactaagga
    31921 tcaaagctta tcgattgaga atgaatacac ctatattatt gctttgttga tgcaacagac
    31981 actgaacgaa gttgctccga accttactct tcatgaagtt aaaccagatc aaatcaatgc
    32041 aagaaatgtc atcttagcaa acgttcaaaa tgatcaaaaa acctatatca atcaagtagt
    32101 tattgaaaca aaaattacaa aaaaaggaca ttctgaagtt ttatatcaag aggaaaaaga
    32161 aggattgcag attgcgccga atacaaattt ttcttttcca actgctttaa atggtcaacc
    32221 gctaacccca ggtgaatatc accttacaat gactgtttta ggaaatgaaa atgaaaatgg
    32281 aaaattttcg agaaaaaaag gaaatactac gatcaactat accaatcaat gggtatttga
    32341 aaaagatttt acaatagacg gaaaagtagc caaggaattg aacacgacgg atgttacgat
    32401 taaaaaagac aataattgga tttatatctt agtagcttca cttttcttat tgtttatttt
    32461 attatgcata cttttgtttg ttttgagaaa aaaagaagag aaagaaaagt aaggttaaga
    32521 taacaaatag taaattttta ttattgtagg atggtt
//
LOCUS       DAZAFF010000023        32531 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000023 DAZAFF010000000
VERSION     DAZAFF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.023"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            272..997
                     /locus_tag="ACG9BB_001041"
     CDS             272..997
                     /locus_tag="ACG9BB_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFN2837007.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            987..1661
                     /locus_tag="ACG9BB_001042"
     CDS             987..1661
                     /locus_tag="ACG9BB_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFN2837008.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(1949..2233)
                     /locus_tag="ACG9BB_001043"
     CDS             complement(1949..2233)
                     /locus_tag="ACG9BB_001043"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFN2837009.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(2527..3834)
                     /locus_tag="ACG9BB_001044"
     CDS             complement(2527..3834)
                     /locus_tag="ACG9BB_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFN2837010.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            4323..5429
                     /locus_tag="ACG9BB_001045"
     CDS             4323..5429
                     /locus_tag="ACG9BB_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837011.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
     gene            6020..6220
                     /locus_tag="ACG9BB_001046"
     CDS             6020..6220
                     /locus_tag="ACG9BB_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN2837012.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            6367..9156
                     /locus_tag="ACG9BB_001047"
     CDS             6367..9156
                     /locus_tag="ACG9BB_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFN2837013.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            9203..9730
                     /locus_tag="ACG9BB_001048"
     CDS             9203..9730
                     /locus_tag="ACG9BB_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFN2837014.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            9751..10941
                     /locus_tag="ACG9BB_001049"
     CDS             9751..10941
                     /locus_tag="ACG9BB_001049"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN2837015.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            10981..12279
                     /gene="asnS"
                     /locus_tag="ACG9BB_001050"
     CDS             10981..12279
                     /gene="asnS"
                     /locus_tag="ACG9BB_001050"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFN2837016.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            13213..13887
                     /locus_tag="ACG9BB_001051"
     CDS             13213..13887
                     /locus_tag="ACG9BB_001051"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFN2837017.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            13884..14225
                     /locus_tag="ACG9BB_001052"
     CDS             13884..14225
                     /locus_tag="ACG9BB_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837018.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            14255..14614
                     /locus_tag="ACG9BB_001053"
     CDS             14255..14614
                     /locus_tag="ACG9BB_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFN2837019.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            14874..14996
                     /locus_tag="ACG9BB_001054"
     CDS             14874..14996
                     /locus_tag="ACG9BB_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837020.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            15138..17219
                     /locus_tag="ACG9BB_001055"
     CDS             15138..17219
                     /locus_tag="ACG9BB_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN2837021.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            17530..18996
                     /locus_tag="ACG9BB_001056"
     CDS             17530..18996
                     /locus_tag="ACG9BB_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN2837022.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            19281..19779
                     /locus_tag="ACG9BB_001057"
                     /pseudo
     CDS             19281..19779
                     /locus_tag="ACG9BB_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            19853..20293
                     /locus_tag="ACG9BB_001058"
     CDS             19853..20293
                     /locus_tag="ACG9BB_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFN2837023.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            20306..20515
                     /gene="copZ"
                     /locus_tag="ACG9BB_001059"
     CDS             20306..20515
                     /gene="copZ"
                     /locus_tag="ACG9BB_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFN2837024.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            20515..22701
                     /locus_tag="ACG9BB_001060"
     CDS             20515..22701
                     /locus_tag="ACG9BB_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN2837025.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            22721..24892
                     /gene="copB"
                     /locus_tag="ACG9BB_001061"
     CDS             22721..24892
                     /gene="copB"
                     /locus_tag="ACG9BB_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFN2837026.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(25004..27175)
                     /locus_tag="ACG9BB_001062"
                     /pseudo
     CDS             complement(25004..27175)
                     /locus_tag="ACG9BB_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(27165..27917)
                     /locus_tag="ACG9BB_001063"
     CDS             complement(27165..27917)
                     /locus_tag="ACG9BB_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFN2837027.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            complement(27914..28237)
                     /locus_tag="ACG9BB_001064"
     CDS             complement(27914..28237)
                     /locus_tag="ACG9BB_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837028.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            complement(28251..29126)
                     /locus_tag="ACG9BB_001065"
     CDS             complement(28251..29126)
                     /locus_tag="ACG9BB_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2837029.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            29453..30742
                     /locus_tag="ACG9BB_001066"
     CDS             29453..30742
                     /locus_tag="ACG9BB_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837030.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            30816..31385
                     /locus_tag="ACG9BB_001067"
     CDS             30816..31385
                     /locus_tag="ACG9BB_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN2837031.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(31561..31737)
                     /locus_tag="ACG9BB_001068"
                     /pseudo
     CDS             complement(31561..31737)
                     /locus_tag="ACG9BB_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            31816..32514
                     /locus_tag="ACG9BB_001069"
                     /pseudo
     CDS             31816..32514
                     /locus_tag="ACG9BB_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT        10771 a         5689 c         6773 g         9298 t
ORIGIN      
        1 aacgatattt ttttttattg gaaaatatcg gtcaagcaat aaaccagttg cataataaaa
       61 tgttaacggc aacaaaatct gtggaaggaa tgaaccggat gatttgatgg aaaaaacaag
      121 atagacaaga agtatacata gtaaaacgtg gaacaagtag gccagaatga aaaatcagtt
      181 tttatccact cgcttttcta tacattcact gtcacaaata taatcgaaag taattctatt
      241 ttgagataaa gtattttata ggagatagta tatgaacaaa aaaagattta aacgtattga
      301 aagaaaattt gcattaactt atgaccaagc taagtatttg aaaacactat ttccaaaaca
      361 ggtagttcgg gataaatata gtgatcattt tatttcttcc ttgtattttg atacaaacga
      421 tcatcacttt ttaaaagaaa tcactacaga aaagtcctat cgagaaaaat ttagaatcag
      481 aaaatatgga gatcctcagg acatatcgcc tatctactta gagataaaaa gaaagtttca
      541 aggaatgaca gataaacgta gaataaaatt agcgcatcat gagctcgatg atatgttgga
      601 aaatatcgaa ttggcagaaa ccacattcag taatttagaa gaagagtata tttttaatga
      661 aatacagtgg ttgttcagaa ggttggcatt ggagcccaaa gtatatatct attatttaag
      721 agaagcattt gtaaatagag attcagaaga tttgcgaatc acttttgatc aaaatatcca
      781 ttaccgtcta atgaacttaa cgacctggga acactgtaca gaagacaatc tggtagcgcc
      841 agaaatcgat gtgctgatgg aagtaaaaac gacaggagaa atacctgaat ggttacaggt
      901 aggattggat aggatgggta aaaaagagat aaaattttct aaatataaac aagtctataa
      961 aagatatatc caagaggacc cgctctatgt tcaatagtgt cttaaaaaat gagccttctc
     1021 aactcacact agatgatttc tttttatgta tggtggtgtc gattttgcta ggcattctga
     1081 ttgcttacac ccatatgtat caaaacaact gtacaaaaaa cttcatgttt gccttgattg
     1141 ggttacctac tttagtacat accgtcattt tgttgatcaa cggtaactta ggcacgggga
     1201 tcgctgttct aggcgcattt aacttagtgc gatttcggtc tgctcaaggg aattcaagag
     1261 atattttcag tattttttgg gcaacgtcga ttggtttagc cacagggacg ggatatattg
     1321 tctatgcact attcgtgtct agtatagttg ctgctatcca agtcatcatc ttcacgcttc
     1381 caatcaaaaa taaacataca gtttttaata ctgaaaaaac gatcaagcta aaagtttcta
     1441 aatccttgtt tctttcgagt attattgaag aaaaaattgc agaatataca gaaaactgca
     1501 atttaaaaaa agtaaaaatc aataaagaaa cttgttcctt acactatata gtaaaagtaa
     1561 aaacgagtat gcttgaaaaa gaattggttc gaacgatcat ggatcatcca gaagtcattt
     1621 cgatcactgt aggtatggat aaatcgaata aattattgtg ataaccaaga aagtacttaa
     1681 caatgaagta agaccccagt tccaaagttt agcggggaca cttcaataaa gtactttctt
     1741 ttttgtcttc agcaactact ggtaaaactg agagcaagct ttcgtgagag tacagcgata
     1801 aagatcaacc tcccttttta tctcgagcaa tttccttgca aggctgtttc gttgatctta
     1861 aacgagttag caacagtcaa agatgcgatg gccataaaaa aattgcatta agttggctgg
     1921 tatctgatag ctgggaatct aaggaagact atccattctg tttcttaaat tccgctagtt
     1981 gatagatatc agccaattgt tccagatgat caacatcaaa gataaccgaa agatgtttcc
     2041 ttttattccc atttgcttga agagttttta tcttctcttt ggttccttca ctcaatgaga
     2101 gtgtgactaa tttctccctc gttttttctg ggaaatgaaa ggatactttg aaaaatccgt
     2161 cccaaataga aagccagaag atcggtttta ctttctgaga gtgggacaaa agtaagaaac
     2221 acttttgtct cattctttaa aaacgaataa acggtgggag cagaagcaac tccttcggaa
     2281 ataagccaaa attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttctcg
     2341 gagttaaaca cttctgtccc aacctcttgt attcaccttt tgatagccaa gcttttccat
     2401 cattataaaa ccgcaaattt caaaaaaaaa cgaacaatac aaaaagcgta ttgctcgttt
     2461 ttctcttcag tttctcacac ctaaacgact tttgtctcga cctctttttg gtgactattc
     2521 atgatgttaa aataaattac tgaaaaaatc tgtgacatgg cgccaaccaa tgacaaaaat
     2581 attagctttt tcaacagtag aagtggtaat gatgtctgtc gaaggctctt ggtttgcttc
     2641 caaatagcct aatttgtcat cagctaattg gatcgttgct gagccaactg tagtgccttt
     2701 gttgatagga gcggtcactt ggtcatccgt tactttcttt ttatcaattg ttggagtaat
     2761 cgttaatttt ccagtatcca ttccattttc gacccagact tttacagggc tttttagcgc
     2821 aacattgacg gaggtttctt ttccatcttt cactttgatg tcttttaggc ttgggatgct
     2881 tgcatttgct tttccaagtt ctttttctga ccagttgtca taacaataat ccatcaatcg
     2941 tgatgtttca ttgaatcggg cacttggatt ttcttcatgg ttatctgcat ttaaaacgac
     3001 tgtgatgatt cgtcggccat ctttgttgat cgttccgaca aagcatgcac ccgcaaaatc
     3061 agttgtgcca gtttttaatc catcgacacc ttcgtgatag tttacaaaac caggtagcat
     3121 ccagttccaa ttgaccattt caaccggtga ctgtgtgttt tcgccgaatg tttgatttgt
     3181 ggtactcgtg atttttaaga cttcaggata atctaagatg aggtgacgag caacaatagc
     3241 gacatctttc gcagacagtt gattttcatc tgtttcacca gtattctcag gtcgattctc
     3301 acctaggtaa gaattgttta aacctgatgc attgacgatc gttgcatctt tgatacccca
     3361 atcttttagt tgtttcttca ttagctcaag aaatttactt tcactgcctg caactttttc
     3421 agctaaagca accatggaag cattggctga ttggatgaaa gctgagtcaa caagttcttt
     3481 cactgtataa tcattttctt tatgcaaagg aacattcgat agatccggca cgacacttaa
     3541 tttttctgca taatccgaaa tggctacctt atcttcccaa gataatttac cctctttgat
     3601 ttgttcaagt acgatatata gaccaatgat ctttgtgatg gaagcaattc ccatcgtttt
     3661 ttcgccatcc tgatcataaa ggatttttcc actttctgca tctacggcaa aggcagcttt
     3721 ggcatttatt ttaaatgcgt cgtctgcttg aacagttgcg ctaggcaaga aagaagtgat
     3781 acataagata agacttgcta atagtacaat aaaacgggta ttttttttga acatctttgt
     3841 tctcctcatt tttaaattga ttcttacaac ttagaatatt atcagagaga acaaaaggtt
     3901 tcaatggttc tgaaaaaaac cttataaaga acaagtctta ttaatgaaga atcatagaaa
     3961 gttatctttt gggttcattg acacgaactt tgactggctg aacttcgggt tcggttaggc
     4021 gtgatgctgc aaaaagggcg cctgctgaga gtgcgaccaa actaaaaatc atgaataatt
     4081 gcataagatc acctcgttct tttttgtttt tttatagtat agcaagagag tttgaagcta
     4141 ctgttaagga aatgagcata taattgatga aagtatgaaa aaaatatgaa gtaggacaac
     4201 acaactaaaa atcgatcagg tatttctctg gtagggattc gttttattgt attgataaaa
     4261 tgggatgtgg tagactttat ttacagatac aaagaaatga tttttttagg aggggtacgt
     4321 ttatggcaaa aaaagaaaaa gaattattat cacaattgac ttcagaaggt accaaaggac
     4381 cgtttgtcac gattatgtta aacacccatg ttgcgcatca agacgtagaa aaagatcaaa
     4441 tcaaattcaa gaattttgca aaggaagcga aaaagcgctt tgaaaagaaa tactctgagc
     4501 atgattggtc tccattccaa gaaaaaatta ctagtttgtt ggacgatccg tctttctggc
     4561 gtagtgccac aacgagtgta gctgtcatag tatcaccaga acaagtgttt atccaccgtt
     4621 tgggtattcc tgtagatgac cagtattatg tgagtgatct accttattta ttagcgatca
     4681 tcaaaaatgg acatttcaac tattcatact atttattagg attgaatcgt gattcgatga
     4741 aattgtacga agtaacgaat aaacaagtca aagaagtggc tttgcctgaa gatgcaccaa
     4801 gagatgtcgt cactgcttta ggatctgaac taacaggagg cgggttgaac tacgcatcaa
     4861 atggtagtga aggtacgacg ttccatggag tcaatcctaa agatgaagaa gtcgaaattg
     4921 attgggtcaa ttattatcaa gcagtcgatg actatttgaa agatacattt gaaaatccag
     4981 agaaatggcc tatcttctta tttgcattac cagaaaatca gacgatgttc aaaaaagtgg
     5041 cgaagaaccc ttattatcgt tccgatgttg cgatctcagc ttcacctgca caactttcat
     5101 taaaagaaat tgaagcaggc gcaaaaaaac tatcgcaaga attagctgtc aaagaagcaa
     5161 gtgactacaa taagttgctt gataaaaagt tcattgatca actcgttgat attataccgg
     5221 cagcaaaaga agggaaaata tcccatttat tcattgctac atctaattta gcagatggtt
     5281 ttggcgaaga tccagaaaca gaatatgacc gccgtcaggt gctgaatgaa ctagcagata
     5341 atgtgatcac gaacggtggt caggtcttta tccttgaaca aaaagatgca ccagacgaaa
     5401 agagtttagt tgcgatctta cgttattgag tgaaaactac caaagtagat gaatggtcag
     5461 gcgttgatag aagtgtatag ctccgagaga aggagtttcc tctgtttcag ccgttggatt
     5521 catcttgagc gagagacaaa agctagcttt tgtctctcgc tcattatttt tgtgctttag
     5581 tcccattggc tttgcgaggg gaagatactt ttctcaaaaa aacgaacctt gcagttgaga
     5641 gggggataga tgcgcatact aatttttatc agttaaaaaa tgatgtggat cattagagaa
     5701 ttccaaagtt cgttcatcct ttttcagata ataaattgaa attttttgca aaagtgaaaa
     5761 tagcaaatag aaattttctg aatcttatag gggtgatttc gagaaaacgt tcgtttattt
     5821 gcaaatatat cttctaaata agagaataaa tacaaaaaaa taaggaaaaa ctctggcaat
     5881 tttgtaaacg gtatgatata gtaagaatgt agtttttgtt cggtaatctg tggtagcttg
     5941 tttcaaataa acatcttcca gatatacacc attcaagtaa ttgcaaaata tgtgttgata
     6001 cacaaggagg atttttatta tggaaacagg tacagtaaaa tggtttaact cagaaaaagg
     6061 atttggattc atcactgcag aaaacggaaa cgatgtattc gttcatttct cagctatcca
     6121 aggcgacgga ttcaaaactt tagaagaagg ccaagcagta actttcgacg ttgaagaagg
     6181 tcaacgcgga cctcaagcaa caaacgttac taaagcttaa tttacctata ttaaaaataa
     6241 aagtccagac atttatgtgt ctgggctttt ttgtcttttt ttccttaaaa agcgatgaga
     6301 gtatttttct gcactttgta gtataatttc aaaagaacat tagaggcagg agaagaggaa
     6361 gatattttga aaccaagaac ttatgcagtc gttgatatcg agacgactgg gacgaatcca
     6421 aaagaagatc ggatcatcca atttggctgc gtcatgattg aagctggtcg gatcacttca
     6481 cgtttttcga tcgatattca tccagggcgg aagatttcaa aacaaatcca gcatttaaca
     6541 gggatcacga atcaacgcgt ccaaaaagcg ccttattttg aagatgtcgc ccaaacgatt
     6601 tacaatcttt tggcagacac catatttgtc gcgcataata tttattttga ttataatttt
     6661 ttgaaccatg aactcatgcg atgcggactt cctagtttga agataccggg catcgatacc
     6721 gtagaacttg cacaaatttt cttgccgacg gaaccaagtt ttcgcttagc tgatttgtca
     6781 gaaagccttg gctttattca tgagaatcct catcaggcgg atagtgatgc cgaagtgaca
     6841 ggtcagcttc ttttactgat cgaagaacga atgaggaaat tgccgatcat cacattagag
     6901 cagattgcac gattaagtag ccatacagga atggatacga gtcgtttcat ttaccacgtg
     6961 cttgaagaaa tgaaagaaaa cccggagccc cttgatctct cattagaaat catcgatggg
     7021 ctagctttgc agaaaaaaga agtggagcta ttcacatccg tccattatgg cgagcggaca
     7081 tatccaagaa aaaaacaagc aaaagaaaaa atgtttggta aaacactgat gtatcgaaaa
     7141 gaacagaaca ggctgatgaa tgctgtctac gaccatttca caaaagatga atcaaaagat
     7201 ttgatgatcg aagcagctac aggaatgggc aaaacgattg gttatctgtt gcctttgagt
     7261 tatttggcta cacctgaaaa accagctgtc atcagcaccg tttcgcttgt tttgcagcag
     7321 cagatcatgg aaaaagacat tccattactg aatcaattgc tagatcagcc gatacaacct
     7381 gtgattatca aaagctatcg ccactatatt gatttacagc gttttaaagg tacacttgtg
     7441 gaaccgccgg gacaaaaaca atatgctttg taccaaatgg ctgtcttagt ttggctaaca
     7501 caaacgaaga ccggtgattt ggatgaatta catttaacca atctgaacca tgcattcttt
     7561 gcagatgtcg cgcacagagg cactgggttt ttagcaagga atcagtcatt ttacaaacaa
     7621 gactttgtgc gtttcttgca aaagaaaatc aaacaaagca attttttgat agtcaaccat
     7681 gcatttttag tacaagaaac acagcgaaaa gaaccattgc ttccagctag tccttatttg
     7741 ttgattgatg aagtgcatca tttgccggaa attgctgaaa aagtaaatga caaacgacta
     7801 agtattaact acttccataa acaaattcag caactggaag agccgaatca gttatttgac
     7861 cgcatacaaa gttatttgcc gaaggaacat gaagccttgc gctggttgtc gatttatcga
     7921 gaagaactct ctgcactatg cgaagcgcag atttgcgtgg cagagggatt aatgaactta
     7981 gtaagcagca agcaacctaa cggacaatat ccagaagaag tcatgatgac aaaagaaatg
     8041 ttggatcagc tgccactgga atctggaaca gcaatggatc atctgcaact tttttatcaa
     8101 gagatccaac aactacaatc acagatgcgc ttgacttttg agattatcca agaaagttgg
     8161 accaaccaga tgcggatcga tttagcaagt ttatatagct tatttgatca attagacaag
     8221 caatacttgc tgatggacca atggctagaa gactggcgag atcatttgat ccattggttc
     8281 gttcccggga atacagcaca gcagatgatt tttcatctcc ttgattttca agcagctatg
     8341 ttgccgtcaa ctgtgtggta tcctcgatat gaccatattc tttatattgg aggaacactg
     8401 aagatcggac ctaatcgaca gtatctctct aaacgacttg gcattgaagc agcacctctt
     8461 aaagtgattg cttctcctta tgattatgag aaacaggcaa gaatctatgt gccgaaagaa
     8521 gcacctgcag tccatcaagt cagcaatcaa gcatatatcg attatttaga agcagcactc
     8581 acaggattga ttgaacaaga agatcggccg atccttgttt tattcacttc tcatgatgtt
     8641 ttgcaaaagg tatatcagaa aatgcatttg tctatgctag aaaaaggacg tgaaattctt
     8701 gctcaaggat ttggcgggag tcgggaaaaa cttttgaaac gcttcatttt gtcagaaaaa
     8761 agcgtcttat ttggtgctga cagcttttgg gaaggcgtag atttacctgg agatgctttg
     8821 cagattgttg tagtcacacg tttacccttc gacaacccgt tacgtccgat ggtcaaagct
     8881 aggaatgctt atctggaatc agaggggatc aatccttttt atcaggaatc cgtgcctaaa
     8941 gcagctttga agttacgcca agcattaggt cgtttgataa ggacggagac agatcgtggc
     9001 gtattactta ttttggatcg gagattagtc actgcaaaat atggtgcacg aatcatccat
     9061 gcattaccga aaaaagtacc gataaaagag ctagctatag atgaaatcta tcaggatatc
     9121 catgaattct taaagaaaaa tgaagaagga agttaaaggt gccgtctttt ttcatcatta
     9181 aaatctgcta taatgaaaag gaatgaagat aaaggagcga cgacccttga agaaaaaaca
     9241 gacaaaggaa aaaacgatta atcgcgtgtt gcttggcttg atcgtatttt tattagcggt
     9301 catcgtgatc agctcgattt tttacttgcg ttccagtcgt ccggcggtcc aggcgaaaaa
     9361 agaagcagta gaaatcgcaa agaaatatgc aaatctcgag gaagccgatc atttttactg
     9421 gtttactcga aagaaaacgt acttttcatt gacgggtacg aatgataaag gaaaagaaat
     9481 cgccgttatc attcctaaat ctggtgaaaa agtaaaagta ttggatcaaa caaagggact
     9541 gactgaaaac gaagcaaaac agacaattgc tgcggcccat cctgaaatcc aaatcgaaaa
     9601 agctgcttta gggatgtatg atgatcagcc tgtgtgggaa gtagtaggaa aagaaacaaa
     9661 tgggaaactg aattattacc tgattggctt tgaatctgga gatgaagtca agacaatcaa
     9721 gggaatttaa aaggtaaagg aatgaatggg atggagttat caaaaagaac aaaacgatta
     9781 gaaccgtctg tcactttggc agctgcggca aaggccaagg cattaaaagc aaaaggcaga
     9841 gatgtgctga gtttgactgt gggagaaccg gattttgcga caccagaaaa catccaagaa
     9901 gcagcaatcg aagcaatccg caatgggaaa gctagctatt atacaccaac agcaggaatc
     9961 ccagagcttc gacaggcaat cgttgattat ctgaagaaaa actatggact tgtctatgaa
    10021 ccttcacaaa cagtcgtaac agacggagcg aaatttgctt tgtataccct tttccaaacg
    10081 atccttgacc cgcaagatga agtcatcatt cctgtgcctt actgggtcag ttacggtgag
    10141 caagtaaaac tggcagaagg acttcctgtc tttgtgaaag gggaagaatc caatagctgg
    10201 aaagttactg ttgagcagct agaacaagca cgttcagaaa aaacaaaagc cgtaatcatc
    10261 aattctccat cgaatccgac tggaatgatt tatagcaaga atgaactgca ggcaatcggt
    10321 gaatgggcag taaaaaatga tattttgata gttgctgatg atatttatgg ccgtttagtt
    10381 tataatggca atgagtttac tccaattgca acgatttcag aagcaatcaa aaatcagacc
    10441 atcatcatca atggcgtgtc taaaacatat gcgatgacag gctggcgcat tggttatgcg
    10501 gtggggaatt ctgagatcat caatggaatg attgctgttg cttcccagtc aaccagtaat
    10561 ccgacagcag tcagtcaata tgcagctgtt gaagcattga aaggcgagca agatacagtg
    10621 gaagaaatgc ggaacgcctt tgaagaacgc ctgaatacgt tatatccgct tgtggcagaa
    10681 ctgccgggtg tttctcttga aaagccacaa ggagcttttt atctttttcc taatgtaaaa
    10741 gaaacgctac gcatgtgtaa atatgaaaat gtcacacaat gggtagaaga tcttttagaa
    10801 gaaacagggg tagcgcttgt gactggcgaa ggctttgggg caccagaaaa tgtccgcatg
    10861 agttatgcca ctgatcgcgc tacgttagaa gaagcggtac gcagaatcaa gcaatttatt
    10921 gagagtaaaa gtcaaaatta atcctgaaga aattcaagga atataaaagg agagtcatac
    10981 gtggaacaaa ttcaaatcat cgattcgaaa aaccatgtgg gagaaacagt aacaatcggc
    11041 gcttgggtcg caaataagcg atcaagcgga aaaatcgcat ttttgcaatt aagagacgga
    11101 acagcctttt tccaaggtgt agttgtaaaa agcgaagtgc cggaagaagt tttccaaaca
    11161 gctaaaaatc tacaccaaga aacatccgtg ttagtaactg gagaaatccg tgaagacagt
    11221 cgttcaaaat ttggttatga aatcggaatt acaaatgtgg aagtggtaag cgagagccac
    11281 gattatccga tcacaccaaa agaacacgga acagacttct tgatggatca tcgtcattta
    11341 tggctgcgtt cttcaagaca acatgcgatt atgcagatca gaaatgaaat cattcgtgca
    11401 acgtatgaat tctttaatca acatcatttt gtcaaaatcg atccgccgat cttgactggt
    11461 tctgcaccag aaggcactac ggatctattc gaaacaaatt actttgatca aaaagcttat
    11521 ttatctcaaa gtggccaatt atacatggaa gctgctgcta tggctttcgg aaaagtcttt
    11581 tcattcggtc cgactttccg tgctgaaaaa tcaaaaacac gacgtcattt gatcgaattc
    11641 tggatgatcg aaccagaaat ggctttcact catcaagaag aaagtttaga agtacaggaa
    11701 caatatgtag cattcttagt acaaaatgtt ttagatcatt gcgaatacgc attagatgta
    11761 ctaggacgag acaaagaagt cttaaaaact tatacaaaat tgccattccc tcgtattact
    11821 tatgatgatg cgatcgaatt actgaagaaa aatggatttg atgacatcga atggggcgaa
    11881 gactttggtt cacctcatga aacgttcatt gcgaattcat tcgaacagcc tgtctttatc
    11941 ctaaactatc caaaagccat caagccattc tacatgaaac cacatccaac aagagaagac
    12001 gtggtgatct gtgcagatat gatcgcacca gaaggttacg gtgaaattat tggtggaagc
    12061 gaacgggcaa cagactatga tttcttgcta gaacaaatcc gtaatcacgg tttggatgaa
    12121 aaagaatatt catggtatct tgatctacgt aaatacggtt ctgttccgca ctctggtttt
    12181 ggtctaggcc ttgaacgaac agtcacttgg attgctggta tcgaccatgt gcgtgaagct
    12241 attccattcc cacgtttgtt gaaccgaatt tatccataag aggttgtgaa atcagtgcct
    12301 tgacctgagt attagagaaa caaaacggaa aaatagcttc tcatatttta ctgttttttg
    12361 agctaatatc gagggtcagc tgattgaaca ccgtttatat caagaggttg tgcgtctgcc
    12421 gttcagctcc gagtcacaag gagtaatttc aaaaaaacat tttttcatgt ttgatgacta
    12481 ttgtgacttg tgaccaagga gttggccgga gagcaacgtt tatttaaaaa aaagaactgc
    12541 gaactctcgc agttcttttt ttttaaatac gggataaagg aagtttccag cagataagag
    12601 aagaaagcaa aagaaattgc tatttgattc acaaggttct ataacgaaat cgcaatcaat
    12661 caaacatata caaagaaggt tattcaaatg gaaatctgat ttgatgatgc ttctgctgtt
    12721 gaaataaagc agcatctagc acctgaaaac aaactaatag taacattcga agatggagta
    12781 ggtccgtatt ttcagcatgc aatgatccat atgtaagttc aattcaccct tgaaatcatc
    12841 ggaccagata aagttatggc caatctatcc caagcaatct agaagagata ttagtaaagg
    12901 aacatttact ggaagtctta gatgagccta taaatatatg ttctgatggc gtcatcaagt
    12961 ggtctgattg acggaagctt aggtgtgatc gatctctctg gaccaaacaa tgaaggaatc
    13021 aaggggaatc cagcacgtta agaaaaaaat agcataaaaa actagacact tgatgagcgt
    13081 tcattcaatt gtttgaaggg actaggctgg tgtcattttt tatacatata ttttctcttt
    13141 cgtcttgata gggcttatgg gataatataa ggaagaaaat catcgtttta ttcaaaaagg
    13201 agtttttgac aaatgaaaaa acgtatatta tttatcggtg acagcattac agacgcagga
    13261 agagaccgag cagaagcaac tgatctaggt aaaggttatc cacttttgat tgccggaaaa
    13321 ctaatctccc aatatccaac gatggaattt gaatgcatga accgtgggat aggaggagac
    13381 aaaattgcag atctgcaagt acgctggcga gaagattgtt tagctttaca acctgactat
    13441 gtatcgattt tgatcggaat caatgatacc tggcataata ctagcgatca ggctatattc
    13501 ggaactgaga aatctgctca gcaatttgaa tccgtttatc gaaaattact aaatgaactt
    13561 caaaaagcgg gaatctgcaa catcatcttg atggagccgt ttgtacttcc tataaccgac
    13621 gaccgtcatg gctggcgtaa agacttagac cctaaaatcc aaattgttcg taaacttgct
    13681 aaggaatatg gcgcattgct ggttccttta gatggcatat tcaatgcttt agggattgct
    13741 tatggttttt cgaaatatac aagagaagat ggggtccacc caacgattgg cggacatgag
    13801 atcattgcac aaagctggat agatgcaatt gccgcagtcg atttttttag tggagataaa
    13861 aatgagcgaa agaaaagtga gaaatgatgg aaatacggtg ggcagtaaaa gaagatctac
    13921 ctcaaatcaa tcagctgatt actgatcatc cagaatcatt cgtaaaagca aaaacggcag
    13981 ctgacctaaa tgataagaat aaactattgc tcgttgctgt aacaaaagaa aaacggatta
    14041 tcggttttgc gcaagcggac gtgcaacagt cagaagaacg ggtgggtcag cgaccatcag
    14101 gaaaactaat ccaacacaca ttagtaacaa aagaaaatac ggaagaaatc caaaaaaaat
    14161 ttgcagaata tttgatgctg gaagctaaaa aaagaagaat aaaatcaatt tttgcccagt
    14221 tttaatcaaa aacagtaata gaaggagaaa tacaatgagt tgtttaggaa atgtcatctg
    14281 gtttatattc ggcggactaa tcggtggtct tagctggtta gcagcgggga tactttggtg
    14341 tatcacgatc ataggtatcc caatcggtat gcaatgtttt aagatcgcct tcctttcttt
    14401 tgcgcctttt ggcaaagaag taatttatca ccagtctggc atgtcattga tcgttaatat
    14461 catctggctg atcgtttcag gcctaccaat tgctctcgca catgtcgtca gcggcatcct
    14521 tctatgtatc acgatcatag ggataccttt tgccaagcaa tctttcaaac ttgctcgatt
    14581 ggcgttgatg ccattcggtg cagaaattgt ataaaagaga ataataaagg ttagataaag
    14641 catagagtca ggtgggatac atgtatttca cctgactcta tgcgttttcc tgtataagtt
    14701 cttccatgtg acttttacgt cttttattta tatggggtta ctgtttatgg aaagttgaaa
    14761 taattttcat taaagaactc tttctatatt tttgaaagcg tagtggagct tcgtatatct
    14821 gggaaggaca ttttgcaggg attgaaaaaa gatattgtcc tatcgaaaaa ttaatgaatt
    14881 tagacaaatt gccagcaaca ggcttcaaac ttagttgcta tcctgtcaag atcaagaagg
    14941 caagtgcggg ttggataaga gcagtggcaa tgatcgaaga gaaaaagaaa gaataagcca
    15001 aaacatctgt atggaagaaa ttttccgatg cagatgtttt tcattttttc tttcggatac
    15061 cttgacatat gaataaccat tcatttataa ttagttatga agagatattc atatgttgag
    15121 gcgaggaggt cattaaaatg aaaagttaca gattagaagg tttagattgt gcgaactgtg
    15181 caatgaaaat cgaaaaagga gttcagaaaa tcaatggagt caaggaagca acggtaaact
    15241 tcacttctgg aaaactaacg attgacgcgg aggaagatca tttggctacg attgaacaag
    15301 aaacaaaaaa agtagtgaag gaattagaac cagatgtcaa agtaacagaa atagataagg
    15361 aaaaagtatc tgaacatgga aatgaaaaag agagaaatac gctgttccgg attttatttt
    15421 cccttgcagg tatcgcttta ttgctattat ttgattttaa tgaactaatt cgcttgatag
    15481 ggtatttatt gatttatctg ctgataggat atgatgtagt gaagaaggca gtgatgaata
    15541 tcgtcaaagg aaaaatcttt gatgagaatt ttcttatgtc tgttgctacg attggcgcta
    15601 tgttgatcgg tgaatatcca gaagccgtag cagtgatgct attttatcaa atcggcgagt
    15661 atttccaagg attggcagtc agtcattcaa gaaaatcgat tcgcgaattg atggcgatcc
    15721 gtccagaaac agctcacgtg cagacggcgg aaggattgat gaccgtgaac cctgaagacg
    15781 tgctgatcgg tcagtttgta cttgtgaagc ctggtgaacg tgtaccgtta gatggaacga
    15841 tcatcgaagg agaatcatta gtcgatacgt cagcacttac aggagaatca gtccctaaat
    15901 ctgtatatgt tggtgaaaca gtattaagcg gatttatcaa taaaaataaa ccatttttag
    15961 ttcaagtaga aaaatcttat gaaaattcta ctatcagcaa attattagaa ttagtagaaa
    16021 atgccagcag taaaaaagca ccagctgaaa atttcattac taaattcgcg cgctattaca
    16081 caccagtcgt tgtaggattg gccgttttat tagctgtttt acctccactt gttgtttctg
    16141 gagcggcgtt ttctgaatgg atctatcgag cattgacatt tttagtgatc tcatgtccat
    16201 gtgcattagt gatttccgtg ccacttagtt tctttggcgg tatcggagga gcaagtaaaa
    16261 tcggtgtatt agtaaaaggt tcaaattact tggagttgct tgcgcaaacc gagacagtgg
    16321 tattcgataa aaccggcaca ttaacgaaag gcgacttttc gatccagacg atcgatacaa
    16381 aaatcgatcc aaagatattc atgcaatata ttgcgagtgc agaacagttt tctacacatc
    16441 cgattgctca atctgtttta gaaggctacg caggtccgct tttaccaaca gctaatatcc
    16501 aggaatttgc aggtgaaggg atcttagctg aagttgatgg caaacaagta ttagtaggta
    16561 atcacaaatt gatggaaaga tttgaaatct cttttccttc aagtcaagaa atcggtacat
    16621 tgctttatct agcaatcgat caaagttaca gtggttatct tgttattgca gataccttga
    16681 aagaagatgc cgtagacgca ttggttcaat taaaacaggc tggtgtgaaa aacacagtca
    16741 tgttgacggg cgactcgaaa aaaattgctg atcatatcgg gaagcaagta ggcgtggaca
    16801 agatatatag cgaattgctt ccagaagata aagtgcagcg gttagaggaa atcttacaaa
    16861 gtaacaataa aaagaccgca tttgtcggag atggaatcaa cgatgcaccg gtgctagcaa
    16921 gagcagatgt aggaatcgca atgggtggat tgggaagcga tgctgcaatt gaagcagcag
    16981 atgtagtcat catgaatgat caaccatcga agattgcaga agcaatccat ttagcaaaga
    17041 aaacattgaa aatcgtcaaa caaaacattg tctttgctat tgggataaaa attttggtac
    17101 tttccttagg tgcttttggt tttgcatcaa tgggagatgc agtatttgca gatgttgggg
    17161 tgacagtatt agctgttttg aatgccatgc gaagcttgca tgtaaaaaat aaacgttgat
    17221 caatgaagag gacgaatgaa cgtcctcttt tttttatatc cttttcttaa tgaaaaatgt
    17281 aaagaaaata gataggatta taaaataact aatgttttta attttgcaga aaattcaaaa
    17341 tagcttgaaa ttcaataagg tcaatgaaat aaaagaaaat aacttatttt tttgatggaa
    17401 aaaatatgaa aaaacgatgt gtattaaata aaataataca cacataaaat aacactgtct
    17461 tgtaagtttg ttaaaaattg aatatgatta catctggata acaaattttt atcataaagg
    17521 agagaacgaa tgaacaacaa acaattacgc tggtttactg ttgcgttaat tgcatttaac
    17581 atggtttggg gcatggggaa tgtagttaat aactatgctc agcaaggagt atcagttgtc
    17641 acctcttggc tattgatttt ggccatttat tttattccat acgcattgat cgttggacaa
    17701 ttaggatcag ccttcaagga tagtaaagga ggcgtcagtt catgggtcga aaatacgaca
    17761 tctaataaac gactggcata ctatgccgct tggacttact gggtcgtcca tattccttat
    17821 ttggcacaaa agcctcaagc aattctgatt gctggtggtt gggcagtcca aggaaatgga
    17881 aatatcgtaa atacgatgtc agtacaaatg gtagcagtta tttcacttat tatatttctt
    17941 gcctttttat atttatcaac aaaaggactt tctactttga aagtgattgg cggactggcc
    18001 ggaacagcga tgttcgtcat gtcgatctta tttattcttt tagctgtaac ggcaccgttc
    18061 atcaatccaa cgatggaatt tgccacacca catatgaatc aagtgaaaac atatattcca
    18121 acatttgatt tcagttattt cacaaccgtt tcgatgctag tatttgcagt tggaggggcg
    18181 gaaaaaatat ccccttatgt caactctaca agaaatcctg caaaggaatt tcctaaaggg
    18241 atgatcttct tagcagcaat ggtagggatt tgtgctgtat taggctcagt cgcaatgggt
    18301 atgctgtttg ctagcgataa tatccctaaa gacttgatgg caaatggggc atacagtgct
    18361 tttcaaattt taggtaatta ctatggtatc ggtaactcat tgatgatcat ttacgcattg
    18421 acaaacatga ttggacaaat ctccgcatta gccttttcaa ttgatgcacc actaaaaatc
    18481 ttgttagcag atgcggatcc agaatttgta ccaaactggc tacgtaaacg taatcgtaaa
    18541 ggtacattgg tgaatggtta tcttctgaca ggtatactag taggaatcat tattttgttg
    18601 ccgatctttg gtatcaaaga tatgaatgaa ttagtaaaat ggttgacgaa cttgaactca
    18661 gttgtcatgc caatgcgtta cctatgggtg ttcttggcat atatgatgtt gaataaagct
    18721 tataagaaat tcaattctga atataaattt gttaaaaatc caaaaatcgg ttttatcttt
    18781 ggtgcttggt gctttgcctt cactgcgttt gcatgtatcc taggaatggt gccaaaagta
    18841 gattatactg ctgatcctaa agcatggtgg ttccaattat tcacgaatat catcacacca
    18901 attgtcttga tcttattagg tatgatcttg ccagcaattg ctcgtcgaga caaaaacaaa
    18961 gaaattactg aaccagaaac agtggttcag ccttaaaata gaaatacttg ttacaagagg
    19021 ttgtgacaga agctacttct ggaacaccgt gaatcagagg ttgtgaaaaa gctgtcctgc
    19081 atcaagtaat aagaacagcc aaacaaaaat agctcctcat attttgccgt tttttgagct
    19141 aatgctgaag gtcagctgat tgaacaccat ttattcgaaa caagggactt tgacacgact
    19201 tttgtcacag tcccttgttt ttttgcggcg ttataagtgg tatgatcgaa ccaaattcca
    19261 aagagaagtg agggagaaga atgaagatca tcgtgattgg acaaagtggg tcgggaaaat
    19321 cgtccctcgc aaggaagatc aaagaaatca ccggctttcc gcattcaggc aaaaaagtgg
    19381 tttttgcaag aacagcaatc gtttatagta tctaatccgt catggatcgt tgaaggaaat
    19441 tatacttcta cgttagagga aaggattaaa gaagcagata agatcatttg gcttaaagtg
    19501 ccgcgttatc tcgccatcta ccgagtagtt tggcgttctc taaaaagaaa gataaacaaa
    19561 aaatctcggc cagatatgcc tgaatcattt tcggagaaac taaacaggaa atacttagaa
    19621 tttttatctt ttatctggca ttttgaagga aacaatgaac caaaaataca gcagctgatc
    19681 caagtagcta atgcaagaaa taaactgatc atccttaaaa caagacgaga gaaacaagaa
    19741 tttttaacta aattgaaaat agagagcaaa aataattgac agtttttgtt tacaagcgta
    19801 atctgtattc ataagctaat gattacattt gtaaacgaaa ggtgtgaaat aaatggaaaa
    19861 agtcaaaacg ccagtaaaga tcagtgattc agagtgggag atcatgcgtg ttatttggac
    19921 acttggacag acgacagccc aagaaatcac gcagatctta gctgattcga tggattggaa
    19981 accagcaacg atcaaaactt tgctgggacg tttggtaaaa aaagaagtga tctggacaga
    20041 acaaaaagga aaaaaattca tttatcatcc agctgtctcg gagatggaaa cagttcgcag
    20101 tgcgacggaa aatctttttt cccatatctg tgcaaaaagg attggagaga cgattgcgga
    20161 tctagtagaa gaagctactt tgactcaaga agatatcaat gagattttga aacaactgga
    20221 acaaaagcaa ccagtagata cgatcgaatg taactgtatc ccaggtcaat gtgaatgtaa
    20281 acaacatcaa taggaggaag aaaaaatgaa acaacaattt tcaatcgaag gtatgagctg
    20341 caatcattgt gcagcaagag tagaagaaac agtaagcgca cttgacggcg tgcaaaaagt
    20401 gaaagtaaat ttgaaaaaag caaacggtac ggtcaagttt gatgaaacaa aagttcaatc
    20461 cgaaaagatt tgtcaagcaa tcaatggatt gggttataaa gcagaggtga tttgatggca
    20521 acagcagaaa ccaaaatgga tacgttcgtc attacaggta tgacttgtgc aaattgttct
    20581 gctaggatcg aaaaagagct gaacggacaa cctggtgtgg tcaatgccac agtcaacttg
    20641 gccactgaaa aagcaagtgt caaatatgaa gggacgacca ctgaaaaatt aatccaaagc
    20701 gtagaaaaca tcggttatgg tgcaattttg tacgatgaag cccacaaaca aaaaattgca
    20761 gaagaaaagc aagcgtattt gaaaaaaatg ctattcgatc ttatattgag tacggttttg
    20821 actttgccgt tgatgttgtc aatgattgct atgatgttag gaagccatgc ggcgattgtt
    20881 cacttttttc attttccaat cgtccagtta gtgctgtctg ctcctgtcca attttatgtc
    20941 ggtgctcgtt tttataaagg tgcgtaccat gcaatcaaga cgaaagcacc aaatatggat
    21001 gtattggtag caattggaac atcggctgcc tttgcattga gcatttacaa cggatttttc
    21061 cgtggacacc cccaagatct ttattttgaa agcagcagta tgatcatcac gctgattttg
    21121 ctcggcaaat atttggaaca tacagcaaaa acgaaaacag gtaatgcaat caaacaattg
    21181 atgtctcttc aaacaaaaac cgcccaagtg ataagaaacg ggaaagaaga aacgttagcg
    21241 attgaagaag tagtcgttgg tgatcaacta gtcatccgtc ctggtgagca gattccagca
    21301 gatggtcgaa ttatttcagg aagcagtgcg atagatgaaa gtatgcttac tggtgaaaat
    21361 cttcctgtag aaaagaatcc ggatgacacg ctttttggcg ggacaatcaa tacgaatggt
    21421 ctgctacaca tggaagtcac acaagtcggc aagcagacag tattagcgca aatcattcaa
    21481 atggtggaag atgctcaagg gagcaaagcc ccaatccaaa aaatcgcaga tcggatctca
    21541 gggatttttg ttccgatcgt attagtcatc gcgtttatca cgctcatagc tacaggtttg
    21601 atcactggtg attggcaact cgcattgatc catagtgtct ctgtattagt cattgcgtgt
    21661 ccatgtgcat taggtttagc gaccccaaca gctattatgg taggaacggg tgttggtgca
    21721 cgaaatggaa ttttaattaa aggcggagaa gcattagaag cagctgctca cttagacagc
    21781 attgttttgg ataaaactgg aacgattaca gaaggaaagc ccaaagttac agatctagtc
    21841 ggttcaaaag aagtcctatc tattttttat actttagaac aagcttctga acatccacta
    21901 ggtaaagcaa tcgtggaata cggaaaactg caagaagcag caacatacga tatgattgat
    21961 tttacagccc acccaggtgc tgggatcagc ggtacgatta atggagtacg ctattttgcg
    22021 ggtacacgta aacgattaat tgaattgaac ctatcatttg atgaatacca agaacacgcg
    22081 ttagaattag aacagcaggg aaaaacagtc atgtttttag ctgatgaaaa gcaagtgatc
    22141 gggtggattg ccgtggctga tcaaatcaaa ctagaagcca aacaagcaat caagcagctc
    22201 cagaataaag gattagatgt ttttatgctg acaggagaca acaaactggc agccgaaacg
    22261 attggtaaac aagttggtat tgatccaaag catatctttg ctgaagtact tccagaggat
    22321 aaagcagcct acgtagaaaa acttcagaaa gatggcaaaa aagtcggtat ggcaggcgat
    22381 ggaatcaatg atgcaccggc cttagcatta gctgatgtcg gtatggcaat gggaagtgga
    22441 acggacattg cgatggaaac agccgatgtg acgctgatga atagcagttt ggcatccatt
    22501 gcacaaacaa tcgagttgtc taggttgaca ttgagaaaaa tcaaacaaaa tcttttctgg
    22561 gcttttgtct ataatacgat tggtattcct tttgccgcac taggcttttt gaatccaatc
    22621 attgcaggag gagcgatggc gtttagttcc gtcagtgtat tgttgaactc cctgagctta
    22681 aatcgacaca tgaaaaagta agagaaggga tggagtaaaa atgaatggga acgatatgga
    22741 gaaaaaacat gagcataaaa aagaagtagt aaacaaagaa agtacagaat ctcatgaaaa
    22801 acagatggag atgaaacatg accatagtca gatggatcat tccatgcaca tgggacataa
    22861 ccatggagat atagcaaaca gcaaacaaat ggaccattcg atgcacatgg atcacgaaca
    22921 cggcggcatg gatcattcca tgcacatggg gaatttcaaa caaaaatttt ggctatcgct
    22981 tatcctagct atcccaatca ttcttttttc accaatgatg ggaatggaat ttccatttca
    23041 agtaacgttt cctggttctg attggctcgt attgatctta gcaactatac tatttatcta
    23101 tggcggtcag ccatttttaa gtggagctaa gatggaattg aaacaaaaaa gtcctgctat
    23161 gatgacactg atcgcgatgg ggattacagt tgcatatatt tatagtgtct attcgtttat
    23221 cgcaaatctg ctcaatccac atacacacgt catggatttc ttctgggaat tggctacttt
    23281 gatcgttatt atgctactag gtcactggat tgaaatgaat gctgtgtcga atgccagcaa
    23341 tgctttacaa aaactagcag aattactgcc agaatcagtg aaacgcttga cgaaagatgg
    23401 aaaagaagag acagtttcat tgaaagaagt aaacgaaggt gatcgtttga tcgtccggtc
    23461 tggtgataaa atgccaacag atggtgtaat cttgaaaggt gaaacaattg ttgacgaatc
    23521 agctgtgact ggcgaatcaa gaggaatcaa aaaacaagcc aatgacaaag tgattggcgg
    23581 ttctatcaat ggggatggaa cgatcgaaat cgaagtaact ggaactggtg aaaatggtta
    23641 tctagcgaag gttatggaaa tggtgcgaaa agcacaagga gaaaaatcca agctagagtc
    23701 attatctgac aaagtggcaa aatggctgtt ttatgtcgca ttaattgtgg gtatcctggc
    23761 attcatcgcc tggttgttct tgacagatct tcctaatgca ttagaacgta tggtaacagt
    23821 ctttatcatc gcctgtcctc atgctctagg tttagctatc ccattagttg ttgctcgctc
    23881 cacatcaata gcagctaaaa acggattact tttgaaaaac cgcaatgcac tggagcaagc
    23941 caatgatcta gatgtgatca tgctggacaa aacaggaaca ctaacagaag gaaaatttac
    24001 tgtaacgggt gtcgaagtac ttgatgatgc gttcaataaa aatgaaatcc ttcaatatct
    24061 aggagccttg gaagcgaatg ccaatcatcc attggctgtt ggcattatga attatttgaa
    24121 agaacatgaa atcaagcctt atcaagctga aaatctaaaa aatctttcag gagtcgggct
    24181 agaggcaaca gtcaaaaaac aacaagtgaa aatagttaat gaaaaagaag ttgagcgtct
    24241 cggattaaac gtagaacaag cattattgaa accttaccaa gaacaaggaa ataccatcag
    24301 cttcctgatt ttagaaaatc atctagctgc aatcgttgct ttaggtgacg tagtaaaaac
    24361 agaagcaaaa gaatttatcc gaacattgaa agaaagaaaa atcactcctg tcatgcttac
    24421 tggtgacaat aaaaacgctg cccaagcagt agcagattat ttagggatcg aagaatatta
    24481 tggcggttta ttaccagatg ataaagaagc tgttgtccag aaataccttg accaagggaa
    24541 aaaagtaatc atggtgggtg atgggatcaa tgatgcgcca agtttagccc gtgcttcaat
    24601 cgggatggca atcggtgcag ggacagatat cgcaatcgat tctgcggatg tcgtattgac
    24661 aaacagcgat ccaaaagata ttttgcattt tcttgatcta gccaagcaaa cgcgaaagaa
    24721 aatgatccag aacctctggt ggggggcagg ttacaatatt cttgcaattc cgctagcagc
    24781 cggaattctt gcgccaattg gtatcatatt aagtccagca gttggtgctg tcttgatgtc
    24841 gttaagtacg gtagttgtag ctctaaatgc tttgacattg aaaatcaaat aataatgaaa
    24901 aaaggttgtg acaaaaaacg tgtcacaacc tttttttcga gtaatcgata ctcaatcagc
    24961 tggttctcgg cattccagaa gtagattctc cagaaatttt atcttatttt ttgaagcggc
    25021 tcccttttgt cagaattttt ttgtataccg tttttcttat tatacgcgtt atttctaaat
    25081 aggttttttt atcgaatttt accggatctg cttgtcgtcc atatatttct gcttcatatt
    25141 gagtagttaa tcgaatgaaa cgcagatcta gcttgaattc ttgttctatt cgcaggctat
    25201 attcctgcaa agattctgcg ggtttgcgcg gtaccaatgt ttccaaaggg ttaagaagtt
    25261 tttgatatgc aatcgtatta gtagaagcaa aaaaatgaag atagataaga taacttgtcc
    25321 agcgcagaga ttttctgaat aacaatagaa agaacaacaa gacggataaa cacactccaa
    25381 ggctgaataa gacgatttta taagctggtg tgagtgttgt ttcttctgat ggaatgacct
    25441 cagatctctc actggaaata gtttctgcca tattttcgct ttcatttggt tgttcgatag
    25501 caggctggac ggtttcggtt gtttcagcaa ctggttgtga acccattgga ttggcaaatg
    25561 aaggggttgg ttcaaatgga agccagccaa atccaggaaa gtatacctct ggccaagaat
    25621 gagcatgact attgagaatg ctgtattcct cattcccttg attatctgtg cctgttctga
    25681 gcccatttgt aaaacctttt gtccagcgag ctgggatacc aagtgttcga agaaggacga
    25741 ccatagaagt tgaaaagttg tcacaatagc cgacttttga atcaaagagg aaataatcca
    25801 cataatcgtg ttctttcgga gtatgcccgg tatccgtttt ggaatagcgg aaatttccgc
    25861 ttgtttttaa atactgttag acagcttcga ctttacgaat taaagttgct tcatttttgg
    25921 tcaattcgac cgccaagtcc tcaatacgtt taggcaaact ttttggcaat tgaagatttg
    25981 tctgctgatt ttctaaatca tcagctttta actgactgct ttttaattct tctctcgtaa
    26041 aaactaacct ttctgtttgt aaagttgcag aacgtgtcga ttcattcggg acaggaatga
    26101 atcgctggct ttcaggcaaa tagtaaagcg tttgtccact agctaatgaa aaatcagtca
    26161 aggtagtatt tccataagga taaggtaaga aagcttgttt ttttagggtc aatgtaatgt
    26221 tttctttgtc ttctataacg gtttggtagc tcgtgatgtc aacagggaat tcttggattg
    26281 gttggatggg tgcgtcctga tcttctatcc atccagaacc ggtgtaatga tttttatttt
    26341 cgatcttcca atagccattt cctttttggt tcgctgtaaa aacaacttcg ccattatcat
    26401 ataaaggtcc accaagctgt tcatcattct cactgaagcc tgttcttgca cttccggtta
    26461 attgatactc atggagtgtg tcataaagcc cttcatcatt aagatattct cggatcggtt
    26521 cactcgtttc ctcgagagat tggtgtactt tcggaagata taaaggcaag atagcgctaa
    26581 aagttaagaa acaaataagc agaactgtag tcaatgactg gaatcgagtg tttcccgaca
    26641 acaaccggat ggagaaaaat tgccttgctg ccagatacac gaacaaaaca gttactaccg
    26701 caatcatttc gtacagtaaa tcttttttcc gaaaaacggt aagcatcatg aagtaagcca
    26761 tcaaagaaag aaagggtgcg taccaatagt cgtaatccac ggtcaatgta ataaataaat
    26821 agatcagcaa cattatgaga aaaaaagctg tcttttctgg caaataataa agtttacctt
    26881 gggatagatg gaagaattcc tctgtaactg acgaaaaaaa ctgtaagatc cataatatgg
    26941 aaaatgtctt cgaaggaaaa tataaaaaca aacaacctat cattactggc aaagaaagca
    27001 gcaaacgtac ccatttttta ggaatacagc agacaataag gcataggaca ccaatagtta
    27061 ggataagaag gtagctgttt tgcaattgat tagctactac aaatatcgga gcacctacac
    27121 ttagtatcac aaaggagtaa acgccacgca gccatttttc ccgtttaatc gtcaatccat
    27181 tcacctcctg gcaatgaaca tatcttgtct ttttcttgga ccatgagttg tttttctacg
    27241 aaataaacaa gataagccgt tcgatctttt gacaatgttt caatagcttc tgagacttca
    27301 ggagaattct caggaacaaa gaggacgatt tgtttttttg catatttttt caagtgtgcg
    27361 aaaaaagctt tctcatcgcc agcttccaaa gaagcaaaat aggtatgacc ggggtctttg
    27421 ccatgaaagg cgcggttgcc taatatcaga agttccggct gcttttcttt tctcaaatgc
    27481 cataaagaaa aatacaagtc tagcagaggt tcaaattttg gactgggcga accccaaaag
    27541 caaaaaagag gagaacattc ttcttcttga taatcgtatt cacggtataa ccactcttga
    27601 gtatgagcag acaatttcca atcgattcgg ttgaaagaat cacctggacg ataagcgcga
    27661 tgttcccgaa aatcaagacc tctttggtac gcgttcagct gctgtttttt ttccagaact
    27721 ctctgtaatt tttctgcttg aatcttacaa aaagcaggca gaacagtcac aggaaaagag
    27781 atctctcttt ttgcactttt tctgaaaagt cctagactat cataggctgt aaaaatgaca
    27841 gttaatgttt catggcatcc tctttctttt ggctgccaaa gcaattggac ttcttgcggt
    27901 ttatttgtaa gaatcaaaat ctcttgtttt tttctgcaaa gattgctggg aagccgattt
    27961 gtaaacgagg gagaggtaaa gaaaaagttc tcttttttgc tatggaaaaa ataacctgca
    28021 tacttttttt gcgataaacg agtggctgca atgtacaagt aaccgaaagt ttctttagaa
    28081 agctgcttaa tgaaatcaag ctccatgata cataaagagt aaaaaaggcc aacaagaacc
    28141 agcctaaaga attgttgaat acaagagcaa aaagcacaat gatgaaatat accagaaata
    28201 acacaaaatt aatgaaataa tctttgattt tttccattgt ctacctcaat ctacctcaca
    28261 ggaataggaa tttgtgcaac aagagtatga atgatttgtt tcttttcgtc ttccttcaat
    28321 gaacgatcgt aaaaaatcag gcgatgggaa aacacgtaag gaactaaatc ctgcaaatct
    28381 ttgggcgtga cgtaatttct cccatggatt aaagcataag cttttgctgc ctgaataaag
    28441 gaaaggcttc ctcgtggact gatgccaagc tggatggcag catgacttct tgttgctgac
    28501 actaactgca aggcataaga tgccacttcg ggactgacaa agacattcgc cacatttgtt
    28561 tttatttctt ctatttcata actgttcaat acttcattta agttatccaa cactttttcg
    28621 aatttatcca aaagtagcaa aagttcgtca tcaaaagaag gataaccgat ttgtagtcgg
    28681 aataaaaaac gatcgagctg tgcttccggc aaaggatatg ttccttcgta ctcgattgga
    28741 ttttgcgtag ctaagacaaa aaaatgattg tctagagggt atgtattgtt gtcgatcgtt
    28801 gcatgattct cggccattgc ttctaaaagt gctgactgag tacgaggagt cgtacgattg
    28861 atctcatctg ccaacaagat cgtggtaaag atcggtcccg gacgaaattc aaactctttt
    28921 gattgtgaat tgtatacgga aaaaccaaga atatcactag gcagcagatc aggagtacat
    28981 tggactctgc tgaaggttcc ctgtacagct tttgctagtg ctttgaccag taacgtttta
    29041 cctacaccgg gaatatcttc gaataagaca tgaccacctg ctaatagggc cacgatcatc
    29101 aattggatag ttgaacgttt gccgatgacc gctttttcta attcttatat caactgatcg
    29161 attttttgtt tggattcttc tattttttct tccattttca catcaccttc tcataaaatc
    29221 gctatttatt tcattgtaat ggaagggaat acatcctaca agcaaagtga tcttattatg
    29281 tttttttaaa gtacattcgt tttgaaatga gaatacgatt aaattgagta aaataaaaaa
    29341 taatgaggaa gatttgcgta tttaaataaa tagaaaggga gttttttatc tttttaggag
    29401 tgaactcatt ttgatcaaac aatatgttta gaaatcaggt gagaaatgat cgatgagatg
    29461 gcaaaaacta ttatcaaaaa atgcacagaa tttattttca ctaatcaatc taatgaaaga
    29521 tttgatctgg cgaaaagctg atttagctga agaactgaaa atcgatgtta aaacagtgga
    29581 tcggcatctg aaacgtttgg cggaaagcga ctgtcccatt cagctgattc ttcataagaa
    29641 aaccgttcag ttagtttatg atcaaggata tagcgaacct tatctcttgt ttgtttttct
    29701 gatccaatca cccccgtttt gtttactgaa aaatctgcta ttaaatgaac cttacgagca
    29761 gaagtacgat cgttctacgc aagagcgatt gagaaagtgg tttggcgagt accacctttc
    29821 tttttcttat aaaaaagcac agatctttgg ttcagaaaga aaagtaagat atctgctgtt
    29881 ttactttttg aaagattttc cgcatttttt tgcagatgat ggagagcagt ccttttctga
    29941 gatgttcatc caattattga agcaaagaaa ggatccggca gaaatcgaga ttttaggaga
    30001 cagtcgaaca ctattctttg aattgtttcc tgaattgcta tttagagagg agcagatttt
    30061 gccaataaaa gaaaaaaatt atctagcaat tttgcgcttg agctgtaacc agattactga
    30121 aaagcggtgg aaacggatag aagagacaca agactatcgg aaattgacga ttgtgactga
    30181 aaagcttgat tcgctgtttt ggctgaatag cgaatttgag agcgaaagca aatcaaagct
    30241 gataaaagta ctttaccaag aatggcttcg ttacgtaatt ggtatccatg accggctgat
    30301 agaagaagtt cgttattatc aggaaacatt agaaaaattt cccaattcca aagataaact
    30361 atatcaattt cggctagcga tccaacaagc acttgcacac cccgcagtag agtgggggat
    30421 gcttttactg aaaacactcc aggaacgagg gtatttagat gtccaccctc ctgaagtgaa
    30481 aatcgtcttt ttttttcgct atgaacacca agaagcaaga agacttgctg gggtactgaa
    30541 caaaacgcta agacatcgaa aacggatctc tatacatgta tgtactcgaa ataagccgcc
    30601 tcggtatgca gatctagtga tcaccgatca tttttttcca ctagaccata acgaagatca
    30661 aaaaacgtat ctgtaccatc cttcatttgg tatcgaaaga cttcttaccg atatcaatta
    30721 ctggttaggc aaaaaccgat aaaacctttg gaaaaaagat caaaacaagt cagtctgtgc
    30781 tataataaaa agcgattagt aaaggaggaa atccattgga attaaagaaa ttagaagttc
    30841 ccacatcttc gatcacagaa gtgaaacgat cgccgatgga tgtatttgct caagcgcgag
    30901 acgcgggaac tggcgtatat atatttaatc gtgaaaaagt agctggcgtg atgctgaccc
    30961 aagaacaata caaaacgctc ttgcaagaac tagatgtcct tcgtcaaaca gtgaaagtgg
    31021 agaggaaaga accaaaagaa ataaacgaag aacctattaa caaaacagct tccgtcgtat
    31081 ccaatgctga agggatggaa gagctggccc aaacattaca gcactcgctc attactggtg
    31141 tgacgattac agcaaaaaat ctagatgaac gaatggtagc actaggtttt attacgaaaa
    31201 agaccggatt tggcggagtc gtggatatgc ttagcgagtt gatggaaact gggaaaatca
    31261 actaccagtt aagaagaaaa ccgcaaaatc ggacaatcat agctgaaatc ataggagagc
    31321 aaaacagtca atctcaattg atggataaaa tcatcatcaa gaaaattttt ctacatgagc
    31381 tataagaaaa ccatgttcgc acttctaggc aagccaggct ttgaagacct tgccaaagaa
    31441 ttgaacgaac gcctttaaga taagggtttt tcgctagatt taaaaaatca gtgatagaaa
    31501 catgttcgct tataaataaa attagtgata gttttagtga tagtgagctt aaaaaagcac
    31561 tcaatttgag tgctttttta tttgctcttc tatatagttt tctagttgat tcattgatct
    31621 ttttttcact tcgggagaaa tatgggcata gacttgtgta gtggaaacat ctttatgacc
    31681 taaaaaatct ttgatatctt ctaaaggtat tcctgcttgt ctcgttctag ctgaaaaagc
    31741 aaaacataat gttggtgttt tcctttcagc atatctatca gcaagatgta gagtaggaca
    31801 acctcgtgag ccaaaagtcc tgtgcaaatc gagttacaac acacacatga taaagaaact
    31861 tttgaaaaga actataaagt atacgttgaa ttggcaagag attcagcaat gaaatatggt
    31921 attccattaa cattagacac tccttataac caaccaggaa tcaaatcgca tttatgggta
    31981 acacaaaata tttggggcga tcatacagat ccttacggtt atctttctga aatgggtgta
    32041 agtaaagaaa aactagccta tgatttggct catggtttta cggatgataa tccaactact
    32101 tcggaggata aaccagtaat tgatccaact cgagcaggtg ctgcaaattc tacgctgaca
    32161 gatggaacaa attacgccca cattgatcag ttcggagaaa tcgaaaacgc aaacttgcat
    32221 gtagctggat ggcacattgc taattataaa tacgagtata ttttcattat ggactacaat
    32281 actggaaaag aactagctag agtaagagct gatgggattt atagaccaga cgtaaatcaa
    32341 gcttataata ctttaggaaa tgttggctat catgtatctt tcaatatgcg tgatttccct
    32401 aataagaaag tatacgtcat gatgcgtgcg acaaacgatc cagaaggaaa tactaagggt
    32461 ggagcacaag attttcatga caagcgttgg tatttaaata ttccacaacg ataaaaaatc
    32521 cccccctctt t
//
LOCUS       DAZAFF010000024        32197 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000024 DAZAFF010000000
VERSION     DAZAFF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.024"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..513)
                     /gene="yajC"
                     /locus_tag="ACG9BB_001070"
     CDS             complement(133..513)
                     /gene="yajC"
                     /locus_tag="ACG9BB_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFN2837032.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(610..1755)
                     /gene="tgt"
                     /locus_tag="ACG9BB_001071"
     CDS             complement(610..1755)
                     /gene="tgt"
                     /locus_tag="ACG9BB_001071"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFN2837033.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1973..2671
                     /locus_tag="ACG9BB_001072"
     CDS             1973..2671
                     /locus_tag="ACG9BB_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFN2837034.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2839..5478)
                     /locus_tag="ACG9BB_001073"
     CDS             complement(2839..5478)
                     /locus_tag="ACG9BB_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN2837035.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6097..7128)
                     /gene="queA"
                     /locus_tag="ACG9BB_001074"
     CDS             complement(6097..7128)
                     /gene="queA"
                     /locus_tag="ACG9BB_001074"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFN2837036.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7321..8496
                     /locus_tag="ACG9BB_001075"
     CDS             7321..8496
                     /locus_tag="ACG9BB_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN2837037.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8500..9141
                     /locus_tag="ACG9BB_001076"
     CDS             8500..9141
                     /locus_tag="ACG9BB_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2837038.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9138..10055
                     /locus_tag="ACG9BB_001077"
     CDS             9138..10055
                     /locus_tag="ACG9BB_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN2837039.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10052..10723
                     /locus_tag="ACG9BB_001078"
     CDS             10052..10723
                     /locus_tag="ACG9BB_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2837040.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11264..11752)
                     /locus_tag="ACG9BB_001079"
     CDS             complement(11264..11752)
                     /locus_tag="ACG9BB_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFN2837041.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11953..12219)
                     /locus_tag="ACG9BB_001080"
     CDS             complement(11953..12219)
                     /locus_tag="ACG9BB_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN2837042.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12219..12482)
                     /locus_tag="ACG9BB_001081"
     CDS             complement(12219..12482)
                     /locus_tag="ACG9BB_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN2837043.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            13003..13143
                     /locus_tag="ACG9BB_001082"
                     /pseudo
     CDS             13003..13143
                     /locus_tag="ACG9BB_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13285..15108)
                     /locus_tag="ACG9BB_001083"
     CDS             complement(13285..15108)
                     /locus_tag="ACG9BB_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN2837044.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15706..18411)
                     /locus_tag="ACG9BB_001084"
     CDS             complement(15706..18411)
                     /locus_tag="ACG9BB_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFN2837045.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18717..19664)
                     /locus_tag="ACG9BB_001085"
     CDS             complement(18717..19664)
                     /locus_tag="ACG9BB_001085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFN2837046.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19854..20123
                     /gene="rpsN"
                     /locus_tag="ACG9BB_001086"
     CDS             19854..20123
                     /gene="rpsN"
                     /locus_tag="ACG9BB_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFN2837047.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20299..20662)
                     /locus_tag="ACG9BB_001087"
                     /pseudo
     CDS             complement(20299..20662)
                     /locus_tag="ACG9BB_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(20673..21794)
                     /gene="alr"
                     /locus_tag="ACG9BB_001088"
     CDS             complement(20673..21794)
                     /gene="alr"
                     /locus_tag="ACG9BB_001088"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFN2837048.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21810..22160)
                     /gene="acpS"
                     /locus_tag="ACG9BB_001089"
     CDS             complement(21810..22160)
                     /gene="acpS"
                     /locus_tag="ACG9BB_001089"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFN2837049.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22290..23801)
                     /locus_tag="ACG9BB_001090"
     CDS             complement(22290..23801)
                     /locus_tag="ACG9BB_001090"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN2837050.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24123..25475)
                     /gene="murF"
                     /locus_tag="ACG9BB_001091"
     CDS             complement(24123..25475)
                     /gene="murF"
                     /locus_tag="ACG9BB_001091"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFN2837051.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25628..26704)
                     /locus_tag="ACG9BB_001092"
     CDS             complement(25628..26704)
                     /locus_tag="ACG9BB_001092"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFN2837052.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26900..28645
                     /locus_tag="ACG9BB_001093"
     CDS             26900..28645
                     /locus_tag="ACG9BB_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFN2837053.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28925..29362)
                     /locus_tag="ACG9BB_001094"
     CDS             complement(28925..29362)
                     /locus_tag="ACG9BB_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFN2837054.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29346..30719)
                     /locus_tag="ACG9BB_001095"
     CDS             complement(29346..30719)
                     /locus_tag="ACG9BB_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837055.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31042..31530)
                     /locus_tag="ACG9BB_001096"
     CDS             complement(31042..31530)
                     /locus_tag="ACG9BB_001096"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2837056.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31552..32154)
                     /locus_tag="ACG9BB_001097"
     CDS             complement(31552..32154)
                     /locus_tag="ACG9BB_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN2837057.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9206 a         6884 c         5942 g        10165 t
ORIGIN      
        1 attatttata tgacacaccc gattattcaa tattgagcaa tatatcccat gctcttatct
       61 ctttcttttt tgaataaaaa atgaggtagg acatctttct gccctacctc gtctttaaat
      121 aagctttgat acttattttt cttccttctc ttctgtctct tccagcgttt ctgttgtttc
      181 agtttttact tcaactgtct cagctggttc ttctgttgtt tcaacagtag tcgttgcatc
      241 atttgttacg acagtccctg gtttgaccgt tttgattgct gcacgatcgt actcaaggaa
      301 gatcccttcg cagtctaaaa caactgtccg tttgtcattg tcgatttctg aaacaactcc
      361 atgtaatccg ccgatcgtta cgactgaatc acctggtttc atgctatcta acaagttttg
      421 acgttcttgc tgttgtttct tttgcgaacg tgacataaaa aaccacattg ctaatagcgc
      481 gataaacatc acaagcattg gtaatgttcc catttttttc acctcactaa ttctgtatct
      541 acaaaaaaca ctttagcaga aaaatgctga taacactagt tttttactta atttttaaaa
      601 gattttcctt tagaagcttt ttgcgttttc tttattgaac ccgtattctt caaaaaaggc
      661 ttctctaaat tcaagcaaat tatcatccat gattgcttga cgtacttgtt tcattagatt
      721 caacaagaag tataaattgt ggtaagaagt caaacgaatc ccaaatgttt catcacattt
      781 gatcaaatgg cggatatagg cacgtgtata gtttttacat gtataacaat cacatttctc
      841 atcaattgga cggaaatcat gggcatattt cgcatttttc actactagac ggccttgaga
      901 agtcatacat gtgccatttc gagcgattcg agtaggcaag acacagtcaa acatgtctac
      961 tccacggata acaccatcga tcaatgaatc agcagcaccg acacccatca agtaacgtgg
     1021 tttgttttcg gggatcaacg gtgtagtgaa ttctaataca cgattcattt ctgatttagg
     1081 ttccccgaca gataacccgc cgattgagta accagggaaa tccatcccga ctagatcttt
     1141 cgcgctttga cgacgaagat cttcaaaccc tgctccttgg atgattccaa ataatccttg
     1201 acggtctgga ttagcatgag ctttcaatcc tcgttctgcc catcgagacg tacgttctac
     1261 tgattttttc acgtagtcgt aactttcatc gaatggtgga cattcgtcaa aactcatcat
     1321 gatatcagag cccaatttat tttggatgtt gattgctttt tctggtgata aaaacatacg
     1381 cgaaccgttc aagtgatttt tgaaatgcac gccttcttct tcgatattcc gcatatcact
     1441 taatgaaaaa acttgaaagc cgcctgaatc tgttaaaatc ggttgatccc agttcataaa
     1501 tttatgcagt ccgccagctt cttctactaa gtcttcacca gggcgtagcc ataaatgata
     1561 tgtattactt aaaataacgc cagcacccat ttctttcaac tcttctgggg acattgtttt
     1621 tactgtagct aatgttccta ctggcatgaa cattggtgtg gggaaagttc cgtgtggcgt
     1681 gatcaattcc cctaaacgcg cgccagtatg tttttctttc ttgattaaac gatagcgaat
     1741 ggcaggttgc gtcattcatt tccctacttt ctatataaaa tatcttgaca actcccacca
     1801 tcataatcgt ttcaagtgct ttttgcaagt tccctcggct tccttctact ttatttaata
     1861 ttcttttttt tatccttttt ttatgaataa caaaaaaaag aaaaacatta tgacagaaat
     1921 attttttgat acatggtata ttggtgttat taaagaaaag gagggaaaaa tcatgcaatc
     1981 ttctgcagaa ttaaaacgcc aagcaaaaga gagtctaaaa ggacgttggg gacaagctgt
     2041 tttactaaat ctaatcccta cattgattgg tattgctact attcttctta ttatgattcc
     2101 tattatcttg tttgccacca ccatatatga tggatcagcg agcgtctcag aagcgacaac
     2161 ttcaggaggc ggtagttcag gaggcggtgg cggaattatt tccagcatca ttagcgcttt
     2221 gttcatgagc ggtatttctt ggacttatct tgatatttta cgtggacaaa aagatcagat
     2281 cgaaccattc aaagatgcat ttcgtggatt caaaggtttg tttatcggcg gagtgatcag
     2341 cttagctgtg ctgatctcga tctttacaac attatggggt ttgctattag ttattcctgg
     2401 aatcattaaa agctattctt attctcaaag ctattttatt tactacgata tcgtccaaga
     2461 aacaggacaa acacctaaag tactggatac gatcactgca agtcgaaaat tgatggatgg
     2521 ttataaagga caacttttct ggttagatct ttcatttatc ggctggcata tcttagcatt
     2581 agcaactgct ggtattggtt atctatggct cagcccatac atttctgcaa caaaagcagc
     2641 attttatgaa caattgccaa aagatattta aaagagagtg tgacttgtga ccgagggggt
     2701 ggcaggagaa caccgtttat cgagaggcag taaaagaaac attcagccca ttagcaaaaa
     2761 ggaggtgtga caacattcat gtcacacctc ctttttgtat agttccagta aaaagaaaat
     2821 caacttagaa acagttaatc attcctcttc ttctgcttcc ggtaatcgat ggaacttgat
     2881 cagttcattc accgcgattg gtacgataga taaaatccct accaagaacc agtgattcaa
     2941 actgatgtgt actaggccga atagttcttg cgtaaatggc agcaaagcaa cagccgttgt
     3001 aatagctaat gccagtaacg ccatttctac caaagcttta ttggtcgcta aattgacttt
     3061 gaagatcgat tggctgctgc gtacattgaa cacatgcaga atcgatgaat aagctaagat
     3121 caagaatgca acagtttgac cgacatgctg acttgcgtct acccatgggc tgatagtatc
     3181 gacataacta cctaaataat aaccaaacaa ggttacgatg gtgaagaccc ctgcatttat
     3241 ggcaattttt tgcaataaac ctcgagcaaa gatcccttca tttttcggaa tgggtggctg
     3301 atccatgatg ccgtgttccg ctggctctct tcctaaagca aatcctggaa gtccatcagc
     3361 aactacattg acaaacagta gttgcacagc tgtaaatggt gctccccatc ctaacagcat
     3421 cgcaatcaaa acaataaaga tctccgaaat attacagctt agcaaaaagt tgatagcttt
     3481 acgaatattc tgataaaccg aacgtccttg actgacagcg tctacgatcg tcgcaaagtt
     3541 gtcatccgtc aaaatcatat ctgcggcact ttgcgccaca tctgttcctg tgatccccat
     3601 ggcacaccca acgttacttg ctttcaacgc tggtgcatca ttcacaccat ccccagtcat
     3661 cgctacgact gcacccgtac gttgccaaga tttgacgatc cggatcttgt cttctggcgt
     3721 tacccgagca tagacagaga gagctttgac acgcgcatct aattcttcgt ctgataattg
     3781 atgcagttct gatcctgaaa gtgcttctga cggatcattc aagataccta attctttcgc
     3841 tatggcactg gcagtcacca catgatctcc agtgatcatg actgttttga tgccagcctt
     3901 tttcgctcgc ttgattgctc ctttactttc tggacgtggt ggatcgatca ttccgatcag
     3961 accaatcaaa cgtaagtctt tttccagtgc ttcagaagaa atatcttgcg gttcttcgtc
     4021 ataagtagca taaccaacag caatcactct cagtgcccgt ttaccaaagc gatcattaac
     4081 gatcgccgct tgttctacat cgcctgaact aaagcgcgga gcaagaacat cgaatgctcc
     4141 tttggtgatt gagatgtatt tttcgcccat ttggtgaact gttgtcatca tttttcgttc
     4201 agaatcaaaa ggaagctcgt ttactcgtgg atatttttct tctaattctg cctttgtatg
     4261 ataattctct tcaacagccc ttacgatggc tacttctgtt ggattacctt tgataacaag
     4321 gtcgtcatct tcttgctcga ccgtcacatc tgtacatagt gcagccattt tcaatatttc
     4381 catcgcgctg ttcgtcattg aatcttcgat attcgttact tcatcttctt tgctccatac
     4441 ccggcggacg cgcatttcat tttgtgtcag cgttcctgtt ttgtctgagc agataacatt
     4501 agcggttcct agcgtttcaa ctgctggcaa ctgtcggatg atcgcatgtt tcctcgccat
     4561 cttctgcaca ccgtatgcaa gtgtcaacgt aacaataaca gtcaaggttt ccggtacagc
     4621 tgcaacagcc aaggaaatcg aagtcatgaa catctcaagg agtggttctc cttgcagttc
     4681 accaatcaca aatactaaag ccgcagccgc taaagcgatc atactgattc gtttgcctag
     4741 ctgattcaat cggacctgta aaggtgtttt ttgcatagta ttttcgttca aaagatccgc
     4801 gatcttaccc atttctgtag ccatgccaat ggcagtgacg atcgctttcc cacgacctgc
     4861 agcaaccgta catcctttaa ataccatatt aagttggtca cctaatgact catcttcttc
     4921 agcaataaat gctgcatctt tttcaactgc ttcactttct ccagtcaacg ccgattcttc
     4981 tacacgtaat tgtgatgctt cgatcaaacg ggcatcggca gaaatagcat caccggactc
     5041 taaaacaaga atatcaccca ctaccagttg ctctgactca acagtggtca ctttgccgtc
     5101 tcgtatgaca gttgccgttt gtttattcat atcttgcaag gattccagtg ctttttctgc
     5161 gtttccttct tggacaatcg ccaataccga gttgatcaca acgatagcga tgatcagcag
     5221 tccttcaaat aaatccccat gttctgtagc aaatgcggta taaaaggaaa tcaccgctgc
     5281 tacaagaagg atgatcgttg taaaatcgga caagcttcga aagaattttt tgatcatcga
     5341 ttctttcggc gcctcatcga atttattcaa tccctgcttt tttcgccgtt catccacttc
     5401 ttgtgaagac aacccagtat ctggatctgc ttctgttacc ttctttactt cttctattgt
     5461 ctgcttgtat tcttccatcc tattcctcct ttaaaatctt cgtacaaaaa aaggtactta
     5521 ttttactata acatcctttt tttatttact tcaaataaag ggcttatcga ttatttgtta
     5581 agaaagcgta aaatttaaag tgcttatatt tgcaaacgct accaaaggat gctattatat
     5641 agatgtaagg aaggaggata ccatcaaata gacagaagaa tttctcgata gaacagtgta
     5701 agcaaacaaa gttgttagaa gtacaggttt cacccagaca tgcgtgtaga gtacaaacct
     5761 ataaatagtg aatgcgtatg gatgtttgga tcacgaagta tccgaaacaa gaagtaatgc
     5821 gatacgtaac aattgaataa cgtaaacgtc tgaaaattga ataggaacaa gagatgtttc
     5881 aaatgttgaa gatgtaacga aaacaaaatt tttgtttaac atgaagaaat tcaacaccgt
     5941 gctttgcatg tatgatggaa agttacaatc atttctgttt atttgaatcc ttccgaggta
     6001 caagaaagcc gagcttatcg agagctcggc tttttctttg tcataaaatc tttgtaaagc
     6061 ttttttgtta ttcaaaacta gcggcttttc ataaccttat ttcacgaaca tcgcatctcc
     6121 aaaactaaag aaacgatagc gctcttcaat cgcatgctgg taagcagata aagtcaattc
     6181 tcttcctgca aaggcactaa ctaacatgac taaagtcgat tttggcaaat ggaaattagt
     6241 tgagaaagct tctaccactt tgaattcata gccaggcgta atgaaaatat cagtccatcc
     6301 gctgtctgct ttgatttctc cattaaattt cgttccaatc gtttctaatg ttcgaattga
     6361 agttgttccg acagccacga ttttcccgcc atttttacgt acttggttca atcgctcagc
     6421 cgcttcttcc gtcaaacggt agaattcact atgcatatgg tgttcttcaa ttttatctac
     6481 gctgacaggt cggaatgtac ctaaccctac atgtagtgtc agatagacta attcaacacc
     6541 ctttgcttcg atctcatcaa gtaattcttt cgtaaaatgc aatccagctg ttggcgctgc
     6601 agcagaacca ttttctttcg catagacagt ttgatagcga tctgggtctt ccagacgttc
     6661 tttgatatat ggaggcaatg gcatttctcc caatgattca agaatctcta gaaaaattcc
     6721 ttcatactca aagtcaatga tccgtccgcc gtgttccaat tcttctttca ctactgcttg
     6781 caatctgcca tcaccaaaag agattcttgt cccaactttg gcacgtttcg caggtttgat
     6841 caatgtctcc cacgtatctc cttgtgtatt cgttagaaga aggacttcta aatgcgcacc
     6901 agtatctggt ttttctccat ataaacgagc aggcagaact cgtgtattat tcataactag
     6961 cgcatcacca ggctgtaact cttcaatgat ttcatggaaa tgtttatctt ccatctctcc
     7021 agttttccga tccaaaacaa gtaaacgaga atgatcgcgg tcttttaatg gtgtttgtgc
     7081 gattaattct tccggcaagt caaaatcaaa atcttctgtt gttaacatat cgtcactcac
     7141 tttctatcat gtgcttcatc attctatcat gtttttcgtt ctgttggcaa aatcagcact
     7201 ttttcacttc tattttttgt tttcctaaat aaaaaaacta ccctatttgt attttttcat
     7261 tgttttaggc atactagtaa tagatagttc ttcattgaaa atagaaaaag aggtggcacc
     7321 atgattgaat ttcaaaatgt gtcaaaaatt tataaaggtg gaaaagtcgc tgtagaggac
     7381 gtcaatcttt catttgaaaa gggtgagttc atttgtttta tcggtacgag cggaagcgga
     7441 aaaacgacga cgatgcggat gatcaaccga atgactgatc caacacaagg gaaaattttg
     7501 attgatggaa aatccatcca agaaattgat ccagtggaat tgcgacgtca gatcggctat
     7561 gttatccaaa gtattggatt gatgcctcac atgacgatcc gtgaaaatat cactcttgtc
     7621 caaaaattgt tgaaagtcag tcaagaagaa cggaataaaa cagctgaaaa aatgattgat
     7681 cttgtggaat tgccccgtga gatgttagat cgctacccac atgaattatc tggtggtcag
     7741 cagcagcgta ttggagtcgt tcgtgccctt gcagcgaatc aagatatcat cttgatggac
     7801 gagccattcg gtgcgcttga tccaatcact cgtgattcct tgcaagacct tgtcaaagat
     7861 ttacaagagc gtttgggaaa aacgatcgta tttgtaaccc atgacatgga tgaagcgatt
     7921 aaactagcaa gccgtatcgc catcatgagt gaaggccgtg tgatccaatt tgatacacca
     7981 gaaaatattt tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg agaagatcgc
     8041 ctactacaag ccaagccaaa tgcgacaaga gtcggcgaag ttatgctaaa tacagcaatc
     8101 acgattacac cggaaaaatc attacaagaa gcaatcaaac tgatgaggga aaaacgtgta
     8161 gatacgttgt tagttaccga taattcgaat gtattaaaag gatttatcga tgtagaaacg
     8221 ttgaataaaa aacgaggaaa agtatccagt gtcggcgaca ttttgaataa acatgttttt
     8281 tacgtaaaag aatccgccta tcttcgtgat accttgcaac gcattttaaa acgcgggctg
     8341 aaatacgtgc cggtcgttga tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca
     8401 ttagtggata tcgtctacga tgtgatttgg ggagaagatc ccgcaactac tgatgttgca
     8461 gagtcagcta ctcctgaaga atctaaggag gtctgaccta tgcagcagtt ttttcatcag
     8521 cacggttcag aaattctctc aaaaggtttt gaacatcttt atatttcagg acttgctttg
     8581 atcattggta tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct
     8641 gccacgattg tgattggctt aaccagtgcc ttgcagacaa tcccttctct tgctttattg
     8701 gcattgatga tcccgctatt tggcgtaggt aaattacctg cgattatcgc actctttatt
     8761 tattcattat tgccgatttt acgtaatacg tacattggga tgaaaaatgt cgattggaat
     8821 tatcgggatg tggcaaaagg catggggatg actgagttcc aatccatttt ttccgttgaa
     8881 ttgcccattg ctatgccaac gatcatggct ggtattcgtt tagctgccgt ttacgtgatt
     8941 gcatgggcta cacttgcctc ttatatcggc gctggtggtt taggcgattt gatttttagt
     9001 ggactgaata attaccaacc agatctcatt ttggcaggta cgatccctgt aacaatttta
     9061 gcactattgg ctgattggct tctaggtctc ttggaacacc ggctgacacc gatcgcactt
     9121 agagaggaga atgaagaatg aaacgtatac taaaaatgac catggttctt ttctctcttc
     9181 ttatacttgc gagttgttcc ttccctggtc ttgccagcaa tacagatgaa gacaccattt
     9241 cgatcacagg tggaatcact tcagaagcac agatcttagc aagtctagtt gccggaatgg
     9301 ttgaacatta tacagataaa aataccgcaa tcatcaacaa tctggctact acaactatca
     9361 accatcaagc gatgatgaac ggagatgctt caatttcagc ggctcgctac acaggaacag
     9421 atttaacgac tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg
     9481 tgaaagatga atttgagaaa agatacggtc aaacgtggtt cccgtcctat agttttgaaa
     9541 atacgtatgt ctttcttgtt cgaaaagaca ccgcacaaaa ataccatcta tctaaagtca
     9601 gcgatctgaa aaatgtagcg gatgaattag tagctggtgt agatacttct tggatcaatc
     9661 gtaaaggaga cggttatgac ggcttccaag aaacttatgg cttctccttc aattcgatat
     9721 tacctatgca gattggactt gtttatgacg cagtcgaagc aggaaaaatg gatattgttc
     9781 ttggctactc tacggatgga cggatcgcca gttatgatct agtcatgttg gaagatgatc
     9841 gtcaattctt cccaccatat gatgctgctc cagttgtaga caacaagata ttagctgata
     9901 cgcctcattt aaaagaagcc ttaaataagt taggtcatac catcagtacg gaaaaaatgc
     9961 aggaattgaa ctacgaagca gataataatc tagtggaacc atcagtagta gcacaacgtt
    10021 tcttacaaga aaaccactat tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt
    10081 tcaacaattt ttctattatt tccaggaaaa tggctcctat atctttgcac aatttatccg
    10141 tcacttcctg atttctatct atggtgtcct ctttgcagca gttgtcggga ttccggttgg
    10201 gatcatgatt tctaggagac gtaaattagc aaactgggtg attcgtttgg cgaatatcat
    10261 ccagacgatc ccttctctag ccatgatctc cattttaatc attggattag gcctgggagt
    10321 caatgtcgtg attgttactg tatttttgta ttccctctta ccgatcatca agaatacgta
    10381 taccgggatg atccaagtag acaaaaacat ccttgatgtc ggcaaaggca tggggatgac
    10441 tgctagacaa cgactattca tggtagagtt accactctct gtttctgtta tcatggcagg
    10501 cataagaaac gcgttagttg ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc
    10561 tggtggtctt ggagatatca ttatccgagg aaccaatgca acagagggca catcgattat
    10621 tttagcaggt gcattgccaa ctgcattaat ggcgatcatc acggactggc ttttaggtat
    10681 tttggaaaga cggctagatc cagccagccg tacatcacgc tgatgttatt gtaaaaaaca
    10741 aaagcttctg cgaccatttg ttggtcgcaa aggctttttt agttctgtca tttttaaggc
    10801 aataatccac tctgattctt ccctccttat gggcaaagag ttgcttttct tagctattta
    10861 tccattccgt atactccaac ttcatactgc caacgggttc atatctgcat ccaccgacta
    10921 agtttttttc ttatccgcta aggcattttg ccaatccatt ttcccatcac tcgtcatgga
    10981 attcatacca catgatctct aaatactcgt ctgcttgttc tttgaattgg gcatcaattc
    11041 ttacatcctt tggcgtggct ttcatttaag ccttttccat cagtcctcga ttgttcgatg
    11101 ccttcagctg catcatcaaa actggtgact gccgtatcgg taacttcgat catcttttat
    11161 acaatcggga acttgccatc ctcatccttg gctaaaaaca tatctagcca ttccagcttc
    11221 tcgccatggg aggtttgcgg ttcctaaaac cggtacacga ttcttatgga ctgcatccgt
    11281 cgcaactaga cttggcgata cgatgatcct tcaccagaag agcacccccg taaaccaatt
    11341 gatcgatata ttgccaataa gaaaagacat tggcgtaaaa gatatcgatc cctcgtgatg
    11401 cattgccgtt gatactgcta ttcatggccg aaagcgccac gactttaact tcttccgttt
    11461 gagtgacatt catgttcggc aatgtcgctt tggatacccg ctctgctaaa ggaacgacac
    11521 tggtattgta tttcgtatct gtatcttttc caaaagacca tgccaacgaa tcctctggga
    11581 accaataaga cgactccggt tgattgtcca ttgctttttt caacatactt ttggctgttt
    11641 ctcgataaac gacatttcca attgtcttcg cctcactttg gcgataatcg atggtcgttg
    11701 ccactgccaa cactaacaac gatgctccaa ttttactgat tttccttgtc acacttcttc
    11761 ctccttgttt tacataaacc cgtcaacatt ccattcctaa actcaaacaa tgacccttca
    11821 aaggtggaga tataggaaca aattaaagaa atcatgcaat aaatccaaga aggttggttt
    11881 tagaacaaaa aaagagtctc ctttttgggg agactctttg ttaactatct aaaaaggttg
    11941 gctcttctca cttcactcat aatgtgtcca ggcagaataa atttctacaa ctttattttc
    12001 ctcatcaact ttgtaaacca ctcgatgctg tctattcagc ctgcgtgagt atctgccttc
    12061 atgtgtaggt ctcagctttt caaaagactg cgtgggcata tatggatctt ccttcaatgt
    12121 ctgaatcact tcttcaaatt gtttctttaa attcgtttgc ttgatttttc ttaaatccac
    12181 tttcgcagag tttttgattg cgactgtata gttactcatc aaagagcttc ccaatcaata
    12241 tcatcgatat tcgtaaagct actgtcgtct ttttcgcggt ctcgtacttt atctaaagta
    12301 cctgtttgct caagaaaaag ggtttcttga atcgctctcc agtcttccag cccaatcaat
    12361 acagcattgt ttccactacg atcactaata atctcgattt cagtatgatt ttcatttacc
    12421 tctttcaata actgataaaa atttttacga gcagtgctag gattcatcgt tgtattagcc
    12481 attgtctctc ctcctttttc tgatataatt atagcgtact tgattaagta cgtcaaaaaa
    12541 atacaataaa gcacttaaat tcagcaaact tttcctcttt tttacataga gaaaaagacc
    12601 cctttttagg gaggatcttt gtccacgctc acttcttact cctttacgac tagcggtagc
    12661 caaacgtaaa gtaattggta acaacgtatc atctttagca aaggtcgaat ccaaagcctg
    12721 tgagtctatg caacaacacc aatacacgaa ggttcctcgt tgcagtgtga cttgattgat
    12781 atcttgaata acctgaggat acgcacgaaa gatttgtaac ggttattcaa aactgatttt
    12841 ggccgtcaag tcttctactg cctcaaaaat gattgcctct ttcgtctcct gtcattcatg
    12901 ttcactatcg atttgatact tgcttagacc gttgggtttg tgaatcacca attaaaaatg
    12961 gcactgagtt atggtaacaa aaaatggtca cttgacgagg ctgagattcc tacttatgaa
    13021 gacacatggc acttaagagt cgaggtaaag gaagaagctg aaaatatagg aggctgtaca
    13081 ttatttggca aaggattcgg aaattatgat caagacatca aaatcaaaat gttttacagc
    13141 taaataggaa gaataataac atttcatacg ctgttcccat ctttaatggc ttatagaggg
    13201 cacctttagc tgagaataag acaaaacaga ccctctagct tcaattaaga gctagagggt
    13261 ctgtttttga ttttaaaact tggtttattt cttcaaatgg tagctatagg tgctgacaac
    13321 gatattttca cgccaagaca atcgcaatct taaccgcaaa cttgtttggt tatggagaat
    13381 attctgccag cgtctgtttg gtacaaattg aggaatcaac acagtagttg tatagttttg
    13441 tttcgctgca tttttactta cgatatctac ataacggata attggatttt caatcgagcg
    13501 ataagaagag tggacgattg aaaaacggac atctgggaaa tgctttttaa attctgcttc
    13561 gatttctttt tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc
    13621 accgatcgaa cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat tcccgactaa
    13681 tacaagaacg gtattcccgt cataatcatg tagttttgca tcattttcta aacgaagctg
    13741 ttctgctact tttttataat gatcatggat cttataaaaa atgaacataa cgattggcat
    13801 aatgatgaag aaaggccaaa tgtcccctaa ccgatagaca aacaaaatcg caatgattgc
    13861 atatgagatg aacgcacccg taatattggc aatcgatttg cttaaccatc tttttccttc
    13921 ttttttccat ttgacgacca tccctgtttg agataaagca aatggaatga acacgccgat
    13981 agagtataac ggaatcaagc gttctgttga gccatggaaa ataaacagca atacgatcga
    14041 acctagcgct agtgtgataa tcccattgga atagcctagt cgatccccac gatcttgata
    14101 catgtgaggc atgaatttat cttttgccag attgtatgca agaactggaa aagcagaaaa
    14161 accggtgtta gcagcaactg ctaaaatcag cgctgtagca aattgaagga cgtaatacaa
    14221 aataccatgt ccaaagacag ctttaccaat ttgagacaaa acagtgactt ctttttcagg
    14281 aacgatccca taccaatagt tgataaatgt gatccccacg aagaagaaac ctaagatcaa
    14341 tgccatcatc gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa agaatggtac
    14401 agcattacta atcgcctcca ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa
    14461 aatcaaagcg atagaaacac ccggcacagc cgctcctgga agtgcagtag cttgcaatgg
    14521 cacagcacct gtcacgatat tatataaacc aacaacgatc agtaacgaga tcacaaggat
    14581 aaatgtgtac accggaaata ataggaacga tgcagattcc ctcagcccac gaagattcag
    14641 catcatcagt agcaaaacga ttgtcacaga aatagcgact tgatgaccgt agagtgctgg
    14701 caccgctgat gtgatcgctt cagcacctgc tgagacagat actgctactg tcaacatgta
    14761 gtcgatcaat aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct
    14821 gacgacatat gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt
    14881 taatgaaatc aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc
    14941 agcagacaaa gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa
    15001 cgcatcagac gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc
    15061 agcagatttt aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc
    15121 tttacacaca attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat
    15181 agtacgctca ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga
    15241 aaaatatcaa gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg
    15301 gctttttctt tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat
    15361 cctttttgat atcttaactt ttggaagaga gatgtaaggc tgctttctac ccatcattct
    15421 tactactcga agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg
    15481 catctgcggt aataagccca accaaattaa aaacataaga agctgttttt atttggtcgt
    15541 tcttattact tgatgcagag cagctttttt acaacctctt ggtacacggt gttcaatcag
    15601 ctgaccctcg gtattagctc aaaaaaacgg caaaatatga ggaactattt ttccattttt
    15661 ttctctaata ctcaggtcaa aacactgatt tcacaacctc tttttttaat gaaaaatatg
    15721 gctctcatgc gccgcttctt tgaatttcgt cattttttct tcgatgaacg gatagattgc
    15781 cgttcctacg attccaaaga caacaaacaa accgatcatg atccagattg ctttcgtagc
    15841 atttggccaa taaattcctc ctgcagattc ccgtaacaag tttaccgcat gagtaaatgg
    15901 caagaacgga ttgatcattt ggaagaattt ccctgagact tggatcggat agttcccgcc
    15961 tccgccagag atcgacagta ctaagatgat aatagcgatc cctttaccga cattatcgaa
    16021 taatgcagcc aagacataga cgatcatcat gaacgctaag gcgatcaaga cagcaaatga
    16081 cacgctgtag accggatttt ggacatctac tcctagtaag aaaatattcc ctaatgtaac
    16141 gatcaagctt tgcgcaatcg ccattgtcaa aaatgtcagc atacgagcaa cgaattgttc
    16201 tcgtttcgta aaattgtttc ggtctttctt tcctaaatag tattctgtcg tagcaacact
    16261 tgaaagaaga acagcgccga cccacagaca tagtgcagta taaaacgggg tgctagctga
    16321 accgttattt gcgattggat aaagcgcatt ggtttgtagc tcaacaggtt gtgtaaagaa
    16381 gtcactttct tttgttgcgt ctagtttcaa taatttgatg acttcgccta gatcgacttc
    16441 ttgttcgcct tttcggatcg cgtctgctgc tttttggata ccagctttga tatttggcca
    16501 gtcattgttg atcaagtccg cagcagcgtt cacagctgtt tcgacttctg gcattttttc
    16561 atttactgtt gtcaatgtac cggtgatttc attttttagt gctggccaat cattttggat
    16621 aaaatcagcg gccaatccta atttttgctg aatgatcggt aattcattgt tgtataggtc
    16681 tgccccttca ttgatgccat tgacgatggt atccatattt ccattcagca ttgtattggc
    16741 atcatggatt tcttgtccaa ttgcaggcat ttctttttga tatttttcta aaattgccac
    16801 tgcattcgtt acagttgctt tcgttgagct gagaagcgaa tcaaagtcga tctggtccgc
    16861 tttgctcagt gcgccttgtg cagtcccaat cgtatcgatg acttggtcta gtagagaagt
    16921 gacagtggaa ctgatttgac ctgtattgat accagcaatg atgccattga tattattaac
    16981 aacggcatca atttgatcta gcaattcatc aatagccttc gtgtcttcat tttgaacagc
    17041 tgtgttcaaa gcagataggc ggttttgcag attgcttagc aagggtttta attgatttag
    17101 ctgatcaata attggctgta atttcgtatt tccagcactt gtttgcaatt tagtcaggaa
    17161 ctcgaccagt ttatcgattg cattttgttg ttgagatatt ccgtcaataa agccgttcaa
    17221 tacattgctg atgtttttct tttcatcatc agtcagctta ccatcttcaa tagctgttct
    17281 tacagtatct attaacgaac ctgtcgttgt tgccacttgt ccaattgact tcaatgtaac
    17341 atctacactg tttgtgatgc ttggtatcgc atctttcagc tgagaggcac ctgattttgt
    17401 ttggtttgct agatcacttg cctgattttc taaatttttc acatctggca agatatcttg
    17461 tacttgttgg atgatcgtca agccttgttt tgcttcatcg attccttggt tcattgtctc
    17521 ttggatcgaa gcaaaatcgc cgtcgatctc tgctagttgc ttaccggcat tttggatctc
    17581 tgggattttt tcttgcaaat caaggatgat ttttgcttga tctttcaact caggcatttt
    17641 gtcattcaat gcaacgactt tttcacccat ctcatctact ttagggatgt aatccacgaa
    17701 ttcatttgct ttattcagtt tttccttgat ttcaggtagt tgactttgta attcgaggac
    17761 ttgttttgtg tagccgtcga tcgtgtccaa attttcatct gttgataaaa tcatatcttt
    17821 aactttattg atacttacta ggttcgtatc gatgtcgtaa cctatctcat tgaatacttt
    17881 caataaagtc gaactggctg ttttgataaa ttcatttgtg atttgcgatt ggatcgaact
    17941 tgctccttta tcggtgattt tcggtgcaat cgcattgatt ttttcattta ccgtatattc
    18001 gatttttggt ttcttgatat caccactagt aaaactcagc agatcttctg agaaatcctt
    18061 tggcaagtaa atacctgcgt aatactttcc ggaacggact ccgtcttcta attgttcttt
    18121 tgaatcaacg aattgccatc ctaattgttt atttttatga agcgattcga tgacttgctc
    18181 cccaatggcc acttctttac cttgaaactc tgctggcttg tctgcactgt aaacagcaat
    18241 tggcagctct ccagtatttc catatggatc ccataatgct ttgatgttaa accacgcata
    18301 tagcgaaggg atgatcatga tcgcgatgat cagaagtgtt gcaatcgggt ttttgaaaat
    18361 ccgtttccag tcaagcaaat ataatcgaag cgtatttttg attgactgca tagttctccc
    18421 ccttcttctt ttttcagctg aattacttta ccatgtattt catacttttt tcaatcggct
    18481 tttttcaaaa aagtcgaatt tttggacata tcacggcaat tgtctcattg taatctctta
    18541 catattgaga aataattaag gattacagcc tttactttat catgaaataa aagggaaaga
    18601 taattttatg ccaaagtttt tatctattat acgtatacgc aaaaaagcta aaacattttt
    18661 tgtctttgcc cctacaaaaa tgtttcagct tttttcgttt tttattcctg tatttcttat
    18721 ttttcgaatg acaggatgat tttcccagca ggaggatgtc cttcaactag ctgatgtgct
    18781 tggatgacat gcttaagtga agcagacaga ttcattgaaa taaaaacatg aaaggctcct
    18841 ttttggtagg ctttgattat gccagcaaag gcttctgcat ctagatattc ctttcttgga
    18901 acaaacgatt cataaaagcc ttcctttttt tgccgcaaat ctagatctgg gaggtcattt
    18961 aatgcaacat acctgccgcc tggtttcatg atctggatgc cggtttctcc tttgatactt
    19021 cctttcgtcg catcgattac aaggtctgcc tgatcagcaa attgcagacc tggatttgtt
    19081 ttatcataag cagcaaatgc tgaagctcct aattttttta ccttttcctc atttttactg
    19141 gatgcactgg ttaagattcg gattcccttt tcatggagaa gctggatcag acttgacccg
    19201 accgcacctg atgctcctag tatcattaca gtatctgtcg gctggatttc cgtcaaatgg
    19261 ttgattagat tataagccgt aattcctggc gtgaccattc cggctgcttc ttcccaactc
    19321 attttatctg gaatttttgc tacttttgct gatggcaaca cgacttcttc tccgtaagtc
    19381 ccgccgactg catggacagc tactcgatca cctacatgaa cagttgtgac atctgaacca
    19441 atctctgtca caataccagc gccatcatta cctggtacat aagggaattt caatgttcgt
    19501 atctctttca tttctcctaa acgcaaggca acatcataag gattcacact gaatgctttt
    19561 atttctacac gaacatgtcc agaaccgact tctctgggat gagcatcgat ttcttcaaaa
    19621 acatcttgtg cttctccgta accgttaatt gcaaatcttc gcatggtcac catctccctt
    19681 tttctttaag tttacgttta tgcctttatt ttgacaaaaa aaatgtcttt tcgttttggc
    19741 gcatgattca aaacaaaata gttgtctttt ttcgcgtaac gtgttaaatt atttatctaa
    19801 aagcgtaaag attacgattt acgctaataa aaaataattg gaggaatttg ttgatggcta
    19861 aaaaatcaaa gattgccaaa gcaaaaaaac agcaagaaac gattgaacgc tatgctgctt
    19921 tacgccagga attaaaagcg caaaaagatt acgaaggctt gcgtaaactt cctttagatg
    19981 ctcgtccaga acgattgaaa aatcgtgatt taatcgatgg acgccctcgc ggatacatgc
    20041 gtaaattcgg gatgtcccgt ataaaattcc gtgaattagc acataaaggt ttgattccag
    20101 gagtaaaaaa agccagctgg taatccaagt ctggctgtaa tgtaaaggag gagggcgcat
    20161 ggaccactcc ttggatcatc aaaaaagata cgttcgcttc ttctagcaga aatgctggta
    20221 ctagaagcag acgtatcttt ttttacgcac agctggtcct tttttatatg taaaggctgc
    20281 atggttactt cgctatattt atttagtaac aggagtcagt gcttcgatac ccacactaat
    20341 tttcaatgct cggtcaactt ctgccataat gggtcgatcc aaatggcaga ccttttcttt
    20401 caatcttgat ttatcgatcg ttctgatctg ctccaacagg ataacggagt ctctttctat
    20461 tcctgttcca gtagctttga tcccaatgtg agtcggaagc ttaggtttag ccatttttgc
    20521 cgttatggcc gcaacaatga tcgtcgggct aaaatggtta cctagattat tttgaataac
    20581 caaaactggg cgtgttccgc cttgctccga tcccacgact ggagaaagat cagcaaaata
    20641 aaatatctcc tctttttacc ataggatcct ccttattctt gatattctct tggtatccgc
    20701 tgtcctaatc cgcaagccac ttcatagtgg atcgtaccta attgatctgc tatatcctgc
    20761 atagtgattt ctttgtctgc atttttgccg ataagcgtca cttttgttcc gacaggaaat
    20821 tcttgaggca atcggatcat cagttgatcc atacatacac gcccaatcgt ttcacagtag
    20881 ttcccttcta ccaatagaga aaaaccctgc atctttcgtg gccagccatc tgcataaccg
    20941 atcggtatcg ttccgatcca ctctgcttca ggagtgatat atgtttctcc atagccgatt
    21001 ccttcacctg ctgggagttt tttgacttga atcaattcag aaaccagctc aagtgctggc
    21061 tgcaatggat aaacttccgg tagtgcgtgc ccagagggat taagtccgta cattgctact
    21121 ccataacgaa tcatattgcc gattgtttca tcatgccata aagccgttgc actattgctg
    21181 acatgtacat atcgaggaag ttcgggaagt tttttcagca cttctataaa gcgttctttt
    21241 tgcagattcc aatatgtatc atctgcctga tctgctgtag aaaaatgggt aaataaacct
    21301 tcccattcta atacacgact ttctttgatg aatgcagctg cctcttttac ttcttcaggt
    21361 gtacaaaatc cgatacgacc catcccagtg tctgctttta gatgaatcga aagaggcgtc
    21421 tctagcttac ttttctctaa aacagcagct gctgcttgca accattcccg tgtacctgca
    21481 gttactgata aatcatgagc aacagctaca gtcagtgcgt cgtaaggaac catattcaag
    21541 ataaggatgg gctgagtaaa tccagcttct ctcaactcga ctccttcatc taaatttgaa
    21601 acacaaaagc ccgaagctcc acctgcaact gctgcctcag ctgtttcaat agctccatga
    21661 ccgtatccgt tcgcttttac tactgcaaac aattcttttc cttgcggaag tcttgcaact
    21721 tcatttgcta cattttcagt tatagctttt ttatgtatca ctgctttcgt tgggcggtgc
    21781 caagcgacaa ccatcgtttt tccttctttc tagtcttcca aaataatttg tgcaaaagct
    21841 gtttcatttg tatgagtaat actgacccat acattgcctt catgaggtga acgcgtcaca
    21901 acaggctgac cattttcatt ttttaaaatc tcgacatctt gaaaggtaac ttttccgatc
    21961 ccagttcccc aagctttaga gaaagcctct ttgcaagcgt aacgcccgcc taaaaattct
    22021 acttggcgat gaaaaggcaa ggactggaat agtttcattt catcagaggt caagattcgt
    22081 gcaatgaatt ttggtttttc ttcgatcagc cgagtgatcc taggaagttc gactgcatcg
    22141 atcccaattc cttttatcat atgcttttct cttttcaata aatttagaac actctcattc
    22201 taccaaatta taaccatttt gccacaaata agtccacaaa taacaaaaat gaaagtttct
    22261 cttaaaaaag agaaactttc attttatgtt taatcgttat ttgaacggat tacaaatccg
    22321 cgttttttat cattgtgacg tttaccgcca tctttttggc tgttattatt tttgctataa
    22381 ccgccttttg atttgtttcc acgataatta ccgccgtcac gacgattacg gttgtttccg
    22441 ccaccacgtt tgccattttt attgtatcct tttttctgca ttggcaatgg gcgttctggt
    22501 gtgatcttca ctggcactgc atcagctgga tcttttgctg tcgtttttag caaaagtgca
    22561 actaaatctt gtgcagaata ttcttctaat aatttatctg ctgtttgcag gtatttatct
    22621 agaccatttt ctgcaagttt tgtttcgatt tgttcaactg cagcacctaa ttgacctttg
    22681 aatgcttctt tttcggttgg tggacgtaaa gtcgtcatcc gttttttcgt caagttttca
    22741 atcacatgca agtagtccat ttcatttggt gtaacgaaag tcacagacat accgcctttt
    22801 ccggcacggc ctgtacgtcc gatacggtga acgtagcttt ctggatcttg agggatatca
    22861 tagttgtaca catgagtgac acctgaaata tctaatccac gtgcagcaac gtctgtcgct
    22921 acaaggatat ctaaatgacc gctcttaaat gaacgcaata cgctcattcg tttttgttga
    22981 gacaaatcgc catggattcc ttctgcacgg taaccgcgtg cttctaatcc gcgtgccaat
    23041 tcgtctacac ggcgttttgt acgtccgaag acgattgtta attcaggtgt ttgaacatca
    23101 aacaagcgag tcatgatatc gaatttttcg tattcttttg cgcggacata gtattgatcg
    23161 atcaagtcag ctgtcatttc tttcgctttg atcttcacat gatgtggatt tttcatgaat
    23221 ttaacgccaa tattcttgat agctggcggc atcgttgctg agaaaagtaa tgtttgtctt
    23281 tgatcaggaa cttgtgaaat gattttttcg atatcttcca agaagcccat attcaacatt
    23341 tcatctgctt catccaaaac taatgtttga acggttccaa gtttcaatgt gtggcggtta
    23401 atgtggtcaa gcatacgtcc tggtgtaccc acaacgatat gtggacgatc tttcaagcca
    23461 cggatctgac ggccaatatc tgctccgccg taaacggctt gtacacgtac ttttttgtcg
    23521 cgccctaaac gatataattc ttcttgtgtt tggatagcta attcacgtgt tggtgcgata
    23581 actaaacctt gtaattcgtg acgatctgga tcgatttttt ctaacattgg taatccaaat
    23641 gcagctgttt tccctgtacc tgtctgtgct tgtccaataa cgtcttttcc agctaatgct
    23701 agtggaatcg tttctgcttg gataggcgtt gcttcttcaa aaccagcgcg ttcgactgat
    23761 ttcaacaatt ctggtgataa ttctagttct ttaaatttca aatgaatcct cctatatggt
    23821 tgttaactga ggatgtagct tttttgctac cccgtcgatt tgttcggtca aagtgtattg
    23881 tttctttttg acagtggcca cgcacgtttt tatccattct tagcccctgt ggcaccaccg
    23941 aaaaatcact ttgcgaagtt taggcattag aaaagccaaa cgggtcgcgt tcagctttat
    24001 cattattatg acatctggct tcaacaagtt gtttctatgg caaaaccagt ccaatttctc
    24061 ttcatctttc ggcaataggt tattctaaca tagtttttcg ttttctgcaa gtttacagat
    24121 ttttatttca tttgctgaag ttgttctacc acttcaaata agcccatgct gttgctgcct
    24181 tttagcataa tactatctgt tgcttcaaga ttttgtttga tagcatcaat caatttattc
    24241 ttttcttttt caaaatagaa cacaggtagt tgggggtatt tttggttcaa ttcttctttt
    24301 agtgcatgca tttgttcccc ataaaggaaa atcatggcga atttctcagg atcgatatgt
    24361 tcactcattt gcgcatgcat atttaaagac tctttcccta attccagcat atctgctaac
    24421 acagcgattc tgcgtccttt tgttggcaga tttttaaaag tatctaatac cagtcccatg
    24481 gctgttggat ttgcattgta gacatcgctc aaaatagctg caccatttcc tgccgttagc
    24541 cattcggttc tgttttgagt gatagaaaca tgggcgagcc cttggcgtat ctcaggtatg
    24601 gacagtccaa agaagcgccc gatcgtatac gcaatcaaag cattcgttac attataactt
    24661 ccaggcagcg ggatcgtaaa tgtttctcct tctactacaa aatcggtttg tgcttttcct
    24721 tttgctgtca cttccgcatg aacatcgcca tttccaatac cgaatgttgt gactgtttgt
    24781 actaatgttg ccgttaaagg tgtcagcaac ggttcatctg cgggcacaac gagtaatccg
    24841 tctgatacaa gtccgtcagt gatttccatt ttggctttag cgattccttc tcgcgaccca
    24901 agattttcta tgtgtgcttc accgatcatt gtgattgctg cagcttttgg ttgagccata
    24961 agtgaaagtt ccgtgatttc attggcgtga tccatgccca tttccaaaac gatcttttct
    25021 gtatcttggg gcatgtgcaa aatcgtgtaa ggaagaccaa tattattgtt gtaatttcct
    25081 tgcgtcttat atgttttgta tttttcagac aagacagacg cggttagatc tttcgtggtg
    25141 gttttaccat tgcttcctgt aattgcaacg acttctgcgc ctactttttc caagtaatag
    25201 gttgccaatt gctgcatggc tttcaaagga tctgctacag gaataactgg aaaatttttt
    25261 ggagcttgtt caacaggcaa gctccaaaat gaagcagatg ctccgtttgt gatcgcttgc
    25321 tggataaatt catgtccatc tcgcgtacca gcaagaggaa cgaataaact tccttcggtt
    25381 atcttccggc tgtcaaattc gacattggta atctttgtca gtgcagagca ttcgctgccc
    25441 acggcttgtg caatttcttg tattgttaga ttcattgaga tctctcctaa aaaaggttgt
    25501 gaaaaaagcg tttggcctaa acggcgggtt cgcatgactc tcacttccaa gtaaatttct
    25561 ctattcgtat ttagaagcta gaatgaacgc ggttacgcaa tcactccagc ctcttcttta
    25621 tttctcttta ttcatttttt tcaaaaaaag attgacgctg atggtatcga ttcattccta
    25681 actggatcag ttcttcaatc aaatcaccgt atttcaagcc catattttcc cataagagtg
    25741 ggtacatact gaactccgta aatcctggca tagagtttaa ttcattcagg aataattcat
    25801 ttttatttgt caaaaagaaa tcgcaccggc tcaatccgct tccacctaac atcgtgtaag
    25861 ctaacttcgc gtactcttgc gctttttgat aaacttcttc tggcacttcg gctggaatct
    25921 gcatttcgat tttattattg atatattttg cttcataatc atagaatgct acgtctttta
    25981 cgacttcacc aggcaaagtc gtccgaacgt cttcatttcc taatacagca acttcgattt
    26041 cgcgcgcttc aattccttgt tcaacgattg ctcgagaatc atactgatag gctgttgcta
    26101 aagcattttg cagctcttct cggttttctg cttttgtaat gccgacacta gaacccatat
    26161 tcgccggttt gacaaacatc ggataaagca aagaaccttc acattgatca aatacttttt
    26221 taggattttc tttccattga ttcttaagta ctggtacata aggaacttgc ggcacaccag
    26281 cagcttgtaa aatatacttg gtcatgattt tatccatggc acatgcactg gtcaatacgc
    26341 ctgcgccgac ataaggcata ttcaatgtct ctaagaagcc ttggatcgtt ccatcttccc
    26401 cgtttggccc atgtaaaact ggaaaaacga tggctccttc ttctttgatt tcgcccggat
    26461 tgatcacttt tcctgtaaag ccttcctctg tctgtccact tgggtcccat gaaagatgca
    26521 agacatcttt gctggcaggt ttttctgtta gtaatggacc tttgacccat tgtccttctt
    26581 ttgtaataaa tacgagttga acttggtaat aattataata aatggcattt aaaactgaaa
    26641 atgcggaaag aatggacact tcatgctctg cgctgcgtcc gccatatagt aaagtaatct
    26701 tcaaaagatt gtcctcctgt attctaaatc gttcagtgat ttactctcat attttagcac
    26761 aaaacaaaaa taaacgaata acttatttat aaaaaaacga gccagcttct aaaagaacga
    26821 gcgtcattta ttttttgtca gctagattat ttgtcccgtt ttttataaaa agttatgata
    26881 aagaaaagga gggatgatga tggcgcaaac aaatccacgc agccgcttgg cagaaatcat
    26941 tcgagtgttc atcaattaca atgtagtgcc aaactttgta cagcaaaaaa atcccgaaca
    27001 ggtaaaaaaa gcctttgaag aactaggccc caccttcatc aagataggac agatgctttc
    27061 tgttcgagaa gatttgcttt cttctgcctt cactcaaaca ttcaaaacat tacaagatag
    27121 tgtacctagc gacacatttt caacggtcaa aaagacgatc gaaacagaac tttctctttc
    27181 attatcagat atattcgatg atttttcaaa atcgcctttt gcctctgctt ctatggggca
    27241 ggctcatcgt gccaagctga agaacggtga cgcggtagtc gttaagatcc aacaccctaa
    27301 catcgccgaa gagatccgac ttgatcttca actatttgaa cgagcgatcc ctttgatcaa
    27361 atacattcct gaaactagcg tagttgatct aaaaggggta ctccaagaag taaaacgttc
    27421 cttgatcaat gaaatggact tcttaaaaga aagtcaaaat ggcgaacaat tctatcaaaa
    27481 gaacaatggc tggaaagaga tccgcagccc caagatttat gacgcttttt gttcgaaaaa
    27541 agtgatcgtg atggaagaaa tgtctggtaa aaacttgaat catttaatga acgccgaaaa
    27601 caaaactgaa acttttatca ctggaattca aaacaagcag ctgaaacaag aagttgcaaa
    27661 gcttttagtt gagaacttta tgaaacaggt gtttgatgat ggttttttcc atgctgatcc
    27721 tcatccaggg aatctgctat tccatgtact aacaaaagaa gaacagacac aagctagccg
    27781 aaaaactgaa acagtccatg aaaaagagtt cggttccttt gcttttcgag cctccacttc
    27841 tgcagaagac cctgttgcgc catataccat caactacatc gattttggta tgatggggca
    27901 tctttctgca ggacttaggc aaaaactcac acaagccgtt ttagcgctgt atacgaaaga
    27961 tgcttatcga atcgaaaaag cggtccttcg actttgtcag caagaaggct cgtttgatga
    28021 aagtcggttc caccaagaac tgacaagttt tcttgaacag tattatgatt ctcctattga
    28081 tgaaatcaac ttgcaagaag tattcacaca tgtcgtgacg atttgtcatc aaaataatct
    28141 gcagttcgat cgtgatatca cgcttttgct gaaagcattt gggacattag aaggggtgat
    28201 ccgcgtcctc gatcctgaag tttccctcat ggaagtcgct tcgccttttg ctcaacatta
    28261 ttttctcact catctggatg tagaagatac attgaaacaa agcggattag acttattgga
    28321 agggatgaag gctgcgccta agatccctca acagctccac catctattag aaatgtggac
    28381 ttcgggacaa ggaaaagtca atttggaatt gaaaaagcaa gacaaattat tgagcaggat
    28441 cgaaagtatg atcaatcggc ttgttttcgg tatgatttta gctgcattga tcgtagggtc
    28501 ctccctgctt gtccaagcag cacctgtaga aaacgcagag gtcgtttcct tattagggat
    28561 atttacgtat gctattgccg cattcgtcat tatttttttg gcaatcgatg cattgatcca
    28621 aatgtataag aagaggaaaa agtagaaaaa ggttgtgaca gaaagaatca gcttcaagaa
    28681 ataagaaggg gttttacata aattgtttct caaatttttg tgaaaattcg gcttattttt
    28741 cgaagaggct acttctgaaa caccgagaat aaaaaatgtg aaaaagctgt tctgcaccca
    28801 gtaataagaa caatcaaaaa gggaacatgg aacacattta tcctgtttcc ctgttccctt
    28861 tttgatgtat tcgagcataa ataaaatacc ttcgttacct actatcttct gtacttggta
    28921 aaccttagtc aattgccttt tttaacgctt gacgaaaaga catatgatct ttctccgtaa
    28981 agggttttgg accctttgtc cttatgccag cttttcttaa ctttccacta tgataacgtt
    29041 gttctagaag cgtgccgata ttcgcttcgt caaattcttt tccagagtga tggaacactc
    29101 gcacaccctt cggcaataca agtgaagcta aaccataatc ttgcgtgatc aaaaagtcat
    29161 tcttcttaac cataccgacg attttataat ccgcactatc agctccgcgg tctacgtaga
    29221 cgatttggac atgattagcg tattctttgg tcgtataatg gtccacactg gtcacgataa
    29281 ccacgtcaag accataggat tctcctaatt ttatgacatc ttcttttact ggagaaccat
    29341 ctccatcaat gaatagcctc attctttttc gccttcttcc ataaatgata aacagttact
    29401 aatccgctca gtaaaagacc aacattgatc cattttgcca agataagcag ttgtacactt
    29461 tctatcggca ctgattcatg gttcagcgtg atcaataata gatggattgg ctctcccatt
    29521 tcttttgcta aaacaaacaa aagcgtcatc aataaaaact ggtggatcac actcccttta
    29581 ttttgagtag tatacttgac aacgatccgg cgatccattg gaatagctgt atcagtttga
    29641 taatattcct tatttagcca gctgcctgtt actacgtgcc agaaactaag cagaaagaca
    29701 cctatcgcaa aaccatttgg aatcaagagg accaataaag aagaactcag tccaagcaat
    29761 tgcatagcca atggagacca tgttaaacgt ttcaaaatcc atggtccgac tatttttgct
    29821 gcaatcaagc ctgctgcata aggcaaatac ataaaaatcg aaagtttttc taatccgtac
    29881 agtatgccaa cataaaaact gctaaataaa aaaaggaaat tccctagcat tccatttaga
    29941 aaagtaaaca gatttagcca gcttggcaac ttccattcct tcttctggtg tcctaaaaca
    30001 aacacgaaca agatgaacag cagcaagaaa ccataaatag caagatcaaa aagcctttca
    30061 tcgaataaca atcgggcatt tctcaaaaga aacaataagc caaagaatat caaaaataaa
    30121 acaagctctc gcgtagctac cagatttctt ttgatgtctt tgaaatccag ttcgtaacgc
    30181 ggataatgac taaccgtatg gtaggccatg acgtaaaaaa gtgtataaat agtaaagctt
    30241 gctgcaatct gagtcggtgc aggcaactca atcgcttgat acaggagaaa tagccagata
    30301 gctgcaaata agtacttttt cccatccata ttgataaaac cttgcgtctt ttcgtgaaaa
    30361 ttgaccgtgg tattagcagg tacaagccat gctgcactca gccctagcaa aacacttgaa
    30421 aaataaaata aaggaaaata aaatacgcct aatattccaa gcaatgaacc agcaccacca
    30481 atcagcagag cgatcatcag caacgtgtaa ctatctagac caaatcgtag actgttgact
    30541 aaaaatagtc cagtcatccg aaaggcataa aacagcgtaa atggcaaaat cgtcagccaa
    30601 ttatgcgagt ctgttagtcc caaaaataaa agataaggaa taaaaataac cgtgttactt
    30661 aagataaagg gcgttgtttg tgtaaaatta cgcccaaatc gtttgagttt ttcgaacatg
    30721 acaatggctc cttaaacagt cttataacta atctgctctt cgattttagc attaaagggg
    30781 aaataaacca attttacaga atatattctt gtaaaaaaaa aatacagaaa gaagcataag
    30841 cttctttctg catttttacg gtacttgatt actcaagcag acaagccctt ctccacaaaa
    30901 gtcttgcccg acacccctgc atccggcagg tgataaagat agaaatcaat tatcctgtgc
    30961 ctccaaaatc aattaggagc tctgtaaacc agagtccttc tcgacccgtc gcataaattt
    31021 actttaacac agttcctatg tttatgcgag agcttttttc attttataat cgaccatttg
    31081 aaatccttga ccgatatctg aaataagttc ttctgtaatc tcaaatcctt catgcttata
    31141 aacctcgatt gcttgatgat tcccctgatt cactcgcaaa aagatctctc tttcctttgt
    31201 ttgcgcagca agttcttcat accagcgaaa aactgaagaa gtcaatcctt gaccacgtac
    31261 tttatttagc aaatataatt tacttaaata aatttttcca tttatctctt cataagcagt
    31321 gtaaccgaca gtcttttctt cgctttttaa taaaaagtag gacacacctt ctgtcagttc
    31381 tttttgaatc tgtttttttg actgataagt tttcagcatg taatccactt gtttttgacc
    31441 aatgatcggc acaaaaactt ccggccatac ttcttgtatg actgccatca gctcatccag
    31501 ttcattttct gttactttta ttttttccat acacccacgt ccttttttac attataaatg
    31561 accttgcgct tgatggaata aaaagagcat gatcatgcta tacacaccca ccgaccacgg
    31621 tttcaagaaa atcacaatct gcataaattc cctaaaagat aatttcgtca aaccagctac
    31681 tagacagatc aagtcgtctg gcgcaaaggg aagcagcaag gtaacaacta gtactttttt
    31741 aatatctttc gtttttcttg tcagcaattc atcaaatttc tgatactttt ttggcgataa
    31801 aataagctgg acaaaggcta cgccatagta acggacaagc aaaaatccga ttgcttcccc
    31861 aatcactagg ccaatacaac tatataaaag cccaaatcca ttgccaaaca tcagcatgcc
    31921 cgctactgag gagatccctc ccggcaaaat aggaatgatc acttggatca cttgcaacag
    31981 aacaaatatc atcactgcat acttgccaaa ttgctggata aacgcttgca aagacgtgct
    32041 cgatcgaaaa atcccatgat gataagcata aagaatcaac acaaagaaaa aaaacagacc
    32101 aataatgggc atgatctgaa gacatttttg taaaactgat ccttttttag acatctatat
    32161 tccccacatc ttttttcatt tattaaaact gcctcct
//
LOCUS       DAZAFF010000025        31092 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000025 DAZAFF010000000
VERSION     DAZAFF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.025"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..1625
                     /locus_tag="ACG9BB_001098"
     CDS             174..1625
                     /locus_tag="ACG9BB_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN2837058.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1974..2606
                     /locus_tag="ACG9BB_001099"
     CDS             1974..2606
                     /locus_tag="ACG9BB_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFN2837059.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3194..4633
                     /locus_tag="ACG9BB_001100"
     CDS             3194..4633
                     /locus_tag="ACG9BB_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2837060.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4928..5146
                     /locus_tag="ACG9BB_001101"
                     /pseudo
     CDS             4928..5146
                     /locus_tag="ACG9BB_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5395..5610
                     /locus_tag="ACG9BB_001102"
     CDS             5395..5610
                     /locus_tag="ACG9BB_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837061.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5827..6083)
                     /locus_tag="ACG9BB_001103"
                     /pseudo
     CDS             complement(5827..6083)
                     /locus_tag="ACG9BB_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6270..7181)
                     /locus_tag="ACG9BB_001104"
     CDS             complement(6270..7181)
                     /locus_tag="ACG9BB_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN2837062.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7200..8204)
                     /locus_tag="ACG9BB_001105"
     CDS             complement(7200..8204)
                     /locus_tag="ACG9BB_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFN2837063.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8201..10324)
                     /locus_tag="ACG9BB_001106"
     CDS             complement(8201..10324)
                     /locus_tag="ACG9BB_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFN2837064.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10329..12776)
                     /locus_tag="ACG9BB_001107"
     CDS             complement(10329..12776)
                     /locus_tag="ACG9BB_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN2837065.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12763..13152)
                     /locus_tag="ACG9BB_001108"
     CDS             complement(12763..13152)
                     /locus_tag="ACG9BB_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFN2837066.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13212..13715)
                     /locus_tag="ACG9BB_001109"
     CDS             complement(13212..13715)
                     /locus_tag="ACG9BB_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFN2837067.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13728..13952)
                     /locus_tag="ACG9BB_001110"
     CDS             complement(13728..13952)
                     /locus_tag="ACG9BB_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837068.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(14056..15966)
                     /gene="ltrA"
                     /locus_tag="ACG9BB_001111"
     CDS             complement(14056..15966)
                     /gene="ltrA"
                     /locus_tag="ACG9BB_001111"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFN2837069.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16712..16849)
                     /locus_tag="ACG9BB_001112"
     CDS             complement(16712..16849)
                     /locus_tag="ACG9BB_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFN2837070.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16846..18030)
                     /gene="mobT"
                     /locus_tag="ACG9BB_001113"
     CDS             complement(16846..18030)
                     /gene="mobT"
                     /locus_tag="ACG9BB_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFN2837071.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18289..18579)
                     /locus_tag="ACG9BB_001114"
     CDS             complement(18289..18579)
                     /locus_tag="ACG9BB_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837072.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18569..19711)
                     /gene="dcm"
                     /locus_tag="ACG9BB_001115"
                     /pseudo
     CDS             complement(18569..19711)
                     /gene="dcm"
                     /locus_tag="ACG9BB_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19787..19951)
                     /locus_tag="ACG9BB_001116"
     CDS             complement(19787..19951)
                     /locus_tag="ACG9BB_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837073.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(19944..20618)
                     /locus_tag="ACG9BB_001117"
     CDS             complement(19944..20618)
                     /locus_tag="ACG9BB_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN2837074.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20696..22036)
                     /locus_tag="ACG9BB_001118"
     CDS             complement(20696..22036)
                     /locus_tag="ACG9BB_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFN2837075.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(22119..22454)
                     /locus_tag="ACG9BB_001119"
     CDS             complement(22119..22454)
                     /locus_tag="ACG9BB_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFN2837076.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(22464..22838)
                     /locus_tag="ACG9BB_001120"
     CDS             complement(22464..22838)
                     /locus_tag="ACG9BB_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN2837077.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22851..23165)
                     /locus_tag="ACG9BB_001121"
     CDS             complement(22851..23165)
                     /locus_tag="ACG9BB_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFN2837078.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(23278..26505)
                     /gene="fss3"
                     /locus_tag="ACG9BB_001122"
     CDS             complement(23278..26505)
                     /gene="fss3"
                     /locus_tag="ACG9BB_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFN2837079.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27362..27616
                     /locus_tag="ACG9BB_001123"
     CDS             27362..27616
                     /locus_tag="ACG9BB_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837080.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            27780..28697
                     /locus_tag="ACG9BB_001124"
     CDS             27780..28697
                     /locus_tag="ACG9BB_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN2837081.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            28690..29472
                     /locus_tag="ACG9BB_001125"
     CDS             28690..29472
                     /locus_tag="ACG9BB_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN2837082.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            29473..30153
                     /locus_tag="ACG9BB_001126"
     CDS             29473..30153
                     /locus_tag="ACG9BB_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN2837083.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            30146..31042
                     /locus_tag="ACG9BB_001127"
     CDS             30146..31042
                     /locus_tag="ACG9BB_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN2837084.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
BASE COUNT         9145 a         6317 c         5598 g        10032 t
ORIGIN      
        1 gttttagcca accctataat tttattttaa aggcagtaac aggattaaac attgaaacat
       61 aatcgttact aaatgaaatc aagaaatata ctgtattact caattcattg aaaatattta
      121 tcataatttt taatagaaaa cactattaaa gagaaaaaag agaggtaaat caaatgaaaa
      181 aatctttaaa acttgcatca gcagttatat taatgtccca actccttata gcaacacctt
      241 tccatgtgtt ggcaagcgaa aattcaacat acaaaattgc aaaagaaaga accgagacga
      301 ctgaaatgag taattccgat gtaacagcag agagtagtga acttttgaca acttcatcaa
      361 gtgaaagtag tacgacttca aatgccgagt cttcaacaac ttcatcaagt gaaagtagta
      421 cgacttcaaa tgccgagtct tcaacaactt catcaagtga aagtagtacg acttcaaatg
      481 ccgagtcttc aacaacttcg tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa
      541 caacttcaag tagtacaagt gaaataactt cttcgaaagt gacaaaacca aaccatatag
      601 aatcttcaaa aaaggaagcg ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac
      661 agcaaataga atcaacagaa tctttccaat cacaaccaat actatctggt aatgaagaaa
      721 ttcattttga caaaaatgaa tctgtagaaa attttattat gaaaattggt gagtctgcga
      781 gaaaaattgg tcagaaatat gatttatatg cttctgttat gattgcacaa gcgatcttag
      841 aatctgccag tggtcaaagc cagttagctc aggcaccgaa ttacaattta tttgggataa
      901 aaggaactta taatggaaac tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa
      961 cgctttatac gacccaatca aaatttagag tgtatgaaaa ctatgaggaa agctttgaag
     1021 attatgccaa attattaact aagggaatta gtggaaataa agatttttat gcaggagcat
     1081 taaaagctaa tagtaaaaca tatcgagaag caactaaatt ccttacagga cggtatgcaa
     1141 cagatacaca atactatttg aagctaaatg aattaattaa aacctacgat ttaaccaact
     1201 atgataaaga agttaaaggt ccacaaatga gtaagactgg ataccaagtg cctattcata
     1261 attattcaat aaccagtttg tttggaaatc gtggaaatga atttcaccga ggacttgata
     1321 tggcagctac acagggtgag ccgattcatg cagttaaagc tggtaaggtt gttaaagcag
     1381 agtttcacta ctcttgggga aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt
     1441 tatatgctca ccagcaacaa tacctagtaa aaactggaga taatgttttg caaggacaaa
     1501 taattggcta tgtaggaagt actgggaata gtactggccc acaccttcat ttggaaattt
     1561 gcaaagatag tagtctttct cagagcatgt tagttgatcc gaaaacctta ttattcggag
     1621 actaaaaaag gtaatcaatc tgacaaagta acaagaaaat ggttggattc ttagaccttt
     1681 gatcaggata ttgagactgg aattgatatc tcgattacga tgggtttgac aaataaaaca
     1741 agtccattat cgaatatcga gtgtattatt gccgtcttta tgctcatatt ttgagcaggt
     1801 ttcactagat gaaaacaatt tatctatctt gaggatttcg tgtacgtatc gttcagtctt
     1861 gtattgtaaa ttagtcataa aatcagacta agaaaaattg gaaatatttt tttctgattt
     1921 attaaaagtt aattgaaatt atatggtact tctttcttgg gagggtaggt gcagtgaagg
     1981 aaagatatgg tcaccttact gttgttgagg agattactca taatggatat aaaaaatatc
     2041 gttgcctatg tgactgtggg aatgaaacga ttgtatatcg atcaaactta ctatcaggta
     2101 gaaccaaaag ttgtggatgt ggaaattata aaaataggca taaatataaa aatttgacaa
     2161 ataaaacttt tggtaagtta gttgcaatca agccaactaa actgcgaagt gaaggaactg
     2221 ttgtttggga atgtcaatgt ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc
     2281 taaaaggtga tgtaaaaagc tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata
     2341 aaaaattcgg attacttact gcaaagtatc ctttggacaa taactgttca tggaaaacaa
     2401 aatgggtttg tcattgtgag tgtggcagta tgtgtgagat aagttatagt aacttatttt
     2461 ttggacatac tcaaagttgt ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag
     2521 ggacagtaat agagtgtcta acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg
     2581 gagtttatcg ttctaaagag aaatagattg cgtatataac tttaaaaaaa aagaggtact
     2641 atttaggatc gtatgattct attaagagtg cagctcaagc tagaaaagat gctgaaaata
     2701 aattgtttca acctttgatt gacaaatatc gaggtaatca ttaattattg tggataataa
     2761 ttaacagtga tatataaaca tctataatat attattcata ctattgatat ttagtataat
     2821 cctatggaat tgaatatcat agcaatgtat ttgaaaagca aattggaaaa caaataggtg
     2881 caacaccata aatatgaaaa aattgggaaa aagtagaatg gaaatttgct tacgtctcac
     2941 gagatagctc acactattaa tctgaagaac aatgaagtcg acttttagaa attgctagaa
     3001 gaaagtaaag cgaattatca ctagttacta tagagtaagt tcaaataaag catctccaac
     3061 ttacatggtt acatgaggat atcaaatcga aattactcaa gcttcctatg gctagagtcg
     3121 cgagaagttg ttgggcagta gacataggtt atattgttct tagctacacg atcattagaa
     3181 aggaaatcct attatgtata gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg
     3241 gattttgatt gctatatcta tgtttacctt catgtccaca ttggatggca gtattgtcaa
     3301 tattgcacta cctaccatct cagatgatat gggcatacca atgaatcaag cggaatgggt
     3361 agtgtccgtt tatctgatga ttgtctgttc ttgtttgttg ctgtttggta aaattggcga
     3421 tagccgtggg aaaatcaaag tatttcggat tggtacgctg atctttgtct tgggatcgct
     3481 gttgtgtggg ttcaatcatt cgctaggtat tttattgttc gcacgggtgg tacaagccat
     3541 tggtgctagt atgactatgg ctacaaatgc cggaatcatc acggaagtct ttcccataca
     3601 tgaacgtggt cgggcattgg gagcaatagg tgcgtttgtt tcattaggct cgattgctgg
     3661 accaggtctt ggcggcttga ttttgtccca attttcttgg gcgtacattt tttggatcaa
     3721 tgtaccggtg ggaatcgtaa cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac
     3781 catgagcggc catacaatcg atggaggggg ctttgctgcc attgcggcta cgattatcag
     3841 cttttttgga gcaatctttt tggggcaaga aagtggcttt ttccatccag tcccccttgt
     3901 actgttcgta atttcagtga ttgcctttgt cagcttttta aaagttgaaa agaaagcagc
     3961 ctctccattg atttctttcg gtatttttaa gaacaagatt tttaccatga gcttgattac
     4021 agccttgttg attttttctt ccaacttctt tgtcaatgta gtgattcctt tttatctgca
     4081 aaatgctcgc gggttgagtc ctagctatgc tgggctgttg atgatggtct ttcctttgat
     4141 gatggtcgtc ggggcgcctt taagcggcta cctgaccgac cgggttggcc cggagatttt
     4201 ggtattagta ggcttgggct tattgtcggt aacccaggtc atgtatatgt ttttgtcagt
     4261 ggactctcct ttgtggctat acattgtcgc tactgccatc atgggtgccg gcaatgcctt
     4321 gttccagtcc cccaataata cgatggttat gagctcggtc actatcgaaa acttgggact
     4381 ggcaggctcg atgaattcct ttgcccgaaa tttggggatg gtcagcggga tttcgctttc
     4441 tacgacggtc ttatatcaag cgatgtctgc taagatgggc cagcaagtaa ctaactatat
     4501 cgcaaatcgg ccggatgtct ttttatatgg aatgaagatt acctttttag gcagctttgt
     4561 tttgtgtttt gttgcctttt tcctgactat ctggcgtatc aaaaaacaag gaggtattca
     4621 gaaaaatgtc tgaaaaaacc actgttgaag aatttttgga ggcctgcgta tgggtgagtg
     4681 ggatttccat ggatgatgga agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt
     4741 atcttcaaaa tactagtgct caatgagatg cttgatttga tcacaagaag actgcgaaag
     4801 ccgctggcaa ccgcaagcta ggtatcccac cgatggacta cgttttagag gctactggtt
     4861 atgaatacgg agccaacgct ctcatctgca taagaataca tcacccagat tatgtaaaga
     4921 ttttcgatac ttctgtcaaa aaatcattaa gatagataaa tgatttccgt ctagtcaaat
     4981 tggttcagaa tgtagacaca cagatggtaa gaagtcactc acaattcgag aatggacttg
     5041 tcctatttgc cgtatacatc ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa
     5101 gggctatgaa tacaagcatt gccttagaaa acatcaagaa ccttaggaac aacggggata
     5161 gcttggttaa taagagatac cgctgtttag gcttgcaagt atactctttt atcaagaatt
     5221 ccgtggcttt agtcatgaga ggcttaatac tatgaaaatt ttctttagca tttttgattt
     5281 aggttccttt tctaattata ataagatgac cactacagta acctactgaa atatataaac
     5341 agccactgta gtaccatttt caaagtagtc tatgagagga gaatcttcat tcttatgaac
     5401 gctggaattg cggatatgaa tttaataaaa aaaaccctta atgattttac ttccaattca
     5461 atatcaaaag gtactggtat caatttaagc acaataaaaa agctgaaaag tggtgaaaga
     5521 tcagtggaaa agctgaatct tttagatgct ataaaaataa cggaatttgc tatgaaaaat
     5581 ggaaaagctg agattgaaat atggagataa ttatcgtcta gtgaattact tgccacttag
     5641 ctgaatttaa cgatccttgg tgcttgaagg gggagattct tgggaataga gcatacttgc
     5701 aagcctatgt ctttactaac aacgatatct cttattaacc aagctatccc cgtagttcta
     5761 aggcttttac ttttatctaa gcaataatcg tattcaaagg aaatttttga cccttagaaa
     5821 gtctcatttt atcagacgtg agcttaggca ttgctgaatt ttgttaattc ggttttaggt
     5881 tgtgctttga gcataatatg aaagtattct ttatcacgat ttcattcatc taaggtaatg
     5941 tgatgtgatt ctgaaattct ttcaaaggca gtttttgcat aagctgacat tacatcatca
     6001 atgacatgtt tacaatgttt cactactaaa ataagggggt aaccaagaag aaaaactgaa
     6061 tgattatttg tatctaattc catggtatat caaccatttc ctatatagac tgattatgac
     6121 atggattaaa ataaaaaggc actatgtctt aattgtcggt tttcgagtta ccgaatggca
     6181 ttcatcaccc acctatagag gatgggcgac ttctgcctga aataagttac actacttata
     6241 acttctaata aaattagatt gagtcattat tatttaacat caacgatttt ccagtttcca
     6301 tttttttgta atactaattt gtactgaaag tagctagtgg tttttgatac attatcaagg
     6361 tattttatct ttgtactcac tagaaaattt tttccgtttt gtagataaac tggattctct
     6421 aactccgaaa actttaagtc aatattgatt ggtcgcatcg caccattttc aacataatac
     6481 tccaattctt tttcagaagc tgatggataa agtttaaaaa atgtttctaa aaaatcggtg
     6541 atttcttccg ttgttttggc atcaatttct gaatcgtttt ctacttgttt tgcttcatat
     6601 tttgccttgt ctggctttcc aatcattgtt ggcaaactag ttactaaatg attcctgttt
     6661 tcatcttcaa agattgtcac tcgatatgca gaagaaattg attgcgtctt ttttccctct
     6721 gtaatatgtt gctccactaa aaatgaaacg acaaattctt ccgagtgttt ttcaacatct
     6781 agaattttta cagattgaac ttcggaactc gtgggaatat ctgctcgaac catatcttgg
     6841 attaaagcga gaccgttttc tgtaaggtac tgttcgagat tactcattct cttttcaatt
     6901 gcttctttgt tatttttcca tgaaaaatat tctttcgcaa aatcctctgt aaaactttca
     6961 acgccagaag tattcactac cttctctttt ataaccactt tttcatgagt tgtatgttga
     7021 tcaatggcgg tgaaattttt ataaacgcca aaagcaagac tacaagacaa taaaagccaa
     7081 agggcaagca ccatttttcg atgcttaccc acggatacaa ctcgttcttt tttctttttt
     7141 gggctctttt ccttttgatt ccgttcaatt tttatcttca ttctttttcc tcttttctct
     7201 tatttgtatc ggccagctcc gataagatgc tgccgccaat agttttccgt tagattcgtc
     7261 caccccactg gatctcctgc atgaaacatg cgattctccc ctgcgtaaat gcccacatgg
     7321 gtcacataag taccggcatc ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca
     7381 tcttttagat caatatgagt catggcatca tattgatcct gagccacccg aggaagtttc
     7441 aggtcaactt tggcataaca ccattgggtc aagccactac aatcaaaccc cgtttctgga
     7501 cttgagccac caaacacata ggccgttcct tcatacttaa atgcctcatc aaaaatttct
     7561 tggactattt tgtcatcaaa ctgagttgta gttaggtatt gcttcactaa taaacagtaa
     7621 aacatattgc cataattata ccgccaccca ccattttcct taatcgcgat tgggttgggg
     7681 taatcgactt tttgaccacc tgatttttcc tttgaaaaag attcagctaa agcaaaacta
     7741 tgcttcttcc cgtttttccc gacatgatct agatacccgc caccatagtt ataggcctga
     7801 ataatactat cttgatcaca ccctagttct ttggaacgct taaccaaaga cgcaaaatag
     7861 agacagcctt gtttgatgga tgcctcggta cttaatgaat taggtggtaa tccggctgac
     7921 tcagaacttt gcataacgtc ttccattgtg ccaccagatt ccacttgaat aatcgccaaa
     7981 agaattggaa tatactctgt tatatccgat tctttggcat acttttccac cattgcacgg
     8041 tgtttcaata cttcttctga gacactagtt ccgccaggag aagtgccaga actaccattt
     8101 ccctcactgt cttcaccaaa caacaaaccc acacacatca aaatacccat aaaagagatg
     8161 gtcagaaaag ttaatgaaat aacacccact ttcttcaagt tcattttttc tcacctgacc
     8221 ttgattttgg acgatggttc attctttttt gtactttttg attggtctgc tttggcagag
     8281 tggacctctg tttcacttgt tggaaagaat ttttcttact tgctgattgg cttttccctt
     8341 ttgaaatgat ttcttgacta gttagttttc gtttgatttc gggattcaga tttgctctca
     8401 caatccgctt tggtgcttct tttttcagca tcatatcttt ctgaggttga gctgctttat
     8461 ctattggaat attcgccctt ctttttccct gacgatttgt tgcttcactc agtatcttgc
     8521 gtttttcgtc cattttcgca tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat
     8581 ccgctttttc ttggaacagt ccgcgcttga attcttctgg tgcttttttc gacttttcga
     8641 ttcctttatg gagattgtat ttcaaattcg ttggcgtatt ccttacctga tcctttactt
     8701 gtttagtctt atctttcacc aaagcttggg tatccaaaac tttgccgatt tttctcccag
     8761 ccaaattcat tcgggattgt ttcttattat ttgaaactgg attctcctta gttaagtcag
     8821 atagtatttc gtgatcgttc ggtaatcgtg aattctttcg tagagaagaa ccggatggct
     8881 taaatttgga ttttgattgt tcctttgcca acttatttcc gactgctgca ccaccaccaa
     8941 aagccaaagc tcgtcccaca tttcgattga gttgccgcaa ttttcgattc attaacattt
     9001 gcggttttcg catgacgcgt cttcccattg actggctatc attactctgg aggttgaaca
     9061 tactcataat gtcgcccaat ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag
     9121 caaccatgaa aaatggatag gttgccgaaa tattgaaaaa catcgtggag atgctaaatg
     9181 cagtcgtaat caccaaagta acacccgctc gcatcataat cgtattgaat aaacgaataa
     9241 tcgccttctt tcctaaactt tcataagtcg gcaacatgga caataagaaa ctaattggca
     9301 gaaacatcgc aaagataatg aagagaattt gcgagaacaa cataatccct gttagaagaa
     9361 aaacaaagaa ggaaatgcca atattgaaca ataccaaaaa gaccacggtc cctaaacggt
     9421 tcatggtctt ggtaatactg agattcgcat tgtcattgtc ttcaatctct gccttgaccg
     9481 cttcttcccg atctttgcct ttgttttcat ctggactcac agatagaatc ttgttgaccc
     9541 ggtcttcacc aatttcttct atgttggaat catcaaattg cagtagtaac caaggttgtt
     9601 ggacttgaat cgaaaacaaa ctatcccgaa tcaaatccac actatctttt ccttggcttt
     9661 ctgaattggg tacaacgatc tcggttccaa gggtaagggc cgcttcacta acatctgagc
     9721 taaagtcatt gatttttgtg atataggtag gcgcataagc aatgaacgaa cccgatagta
     9781 gaaaaatcat cacaaaatta atcacggctc gaactgcttt cgtggtttct cgttttaata
     9841 atccggtata cgccacatac acccccagag ctaaaatcat caataataga aacccaaaat
     9901 aaaatccgga ggtctggagt ccattttcag taataccagc caatgtttgg atattctttc
     9961 caatactttc agccgtatcc gaaatgaaat ctaatttgta agcctcttga accacataac
    10021 ccgtggcgtt cgacaaatac aaactcaccg tccaaatgaa attagtaatg gcataaagac
    10081 catacatcac gcttttccct aagccatcac cccaattcca aggcaaccaa tcccaagagc
    10141 tatccacaaa gaaatctagt tgatagttgt tcagtgaata tttggaatat agatttcctg
    10201 cttcaactgt atcatcaact agccctgtcg cctcggctac cgtaccaact aaactcaata
    10261 gaactatcag agtgagtatg ctaataaaaa cgattcccac aatccgaaaa agtttctttt
    10321 tcatcccctc actccattct ctcttgaaca ggtggacggg tatcaaaggc atggaagagt
    10381 tcttcaaaga ctggatgtac ctgaaccacg cctacccgac catacaaatc ctgcatcaag
    10441 cattggccat tttccaaatc tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag
    10501 ccaaagaatt ccaaggtttt cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc
    10561 aacccgatat tattcttcaa gcgttcatcc agtaaatcat cactattttg cgtgacaaag
    10621 taaaccccgg cattcatagc ccgcccagca cgaacgagct tattcgataa tgttttccct
    10681 tgcgctactt gaagaaaact ccacgcctca tctaagtcta caattttgaa gatttctcga
    10741 ttactatgga taaagtccaa ggcaaaagtg gaaatgacga ttagcatgga aacactaagc
    10801 aattccatgg tggtatattc ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct
    10861 acttgaatga tatttaactg cctatctaaa ctaatcgact gggtaatcga accatctgag
    10921 aaaagcaaat gggcaaaatc ataatcggta aaggactcga tgtgatccgc gatattttcc
    10981 gcaactggtg agccttcttt tcgaagctca ttaattactt gtaataatcc ccgttgattg
    11041 ctttgggtaa ccgtccgaat ggccttacgc aacactggaa acttctcccc atctcgacta
    11101 gaaattcctg ttaaaaaggt cagaatatca attgctagac tttcagaatc cttctttttc
    11161 tccataatca cataaggatc aagtaatcct tgattttttg gcttactagt taagttgaga
    11221 atattgattt catccgcaat ttctggcaag gtttctttcc agccaccccg ttcccctttt
    11281 ggatcgacaa tcacagcttg tccaccaaaa aggacggcgt aataaactaa gagattgtta
    11341 ctaaaggact ttccgccacc gagtgaaccg aggaatgccg ctgctagcgc attggtaact
    11401 gaatccttga ccccttgtga ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct
    11461 acgttataac caaagtaaat gccttccaac tcccctaaca tctgagtggc gccaaaacca
    11521 agtccggcga gaaaatcaga cgtaacatac tggatataat cattcatgta tcgcttacta
    11581 gaaggtaaga attcttcatg tagccccatc atatcgccaa aaggccggac tagttttaca
    11641 ttggtatcat cataaaaatc tagcacttca tcacaccggc gctttagttc atctactgat
    11701 tcggcactta ctcgaaccac atagctcagt ttgtacatgg attctttgga ctgatccaag
    11761 gtagtttcca attcatctac ggaatccaat gcatctaaga cattggaatt tgtttcattg
    11821 tcagattgat aggcatggtt gtccaagtct tttaattcct ttttcttatt gcgaacggta
    11881 gcaagtgcct ttttgttcgt cacaatctcc acattcatac tagtgtcaat tgggaatgta
    11941 aattgttgtt gctggtaata gaaaagttct gaggagggaa actccatttc ccccacaatt
    12001 gtattaattg ttaagtacgc tacatatgat tcatgattct catgttccat gcataagtac
    12061 cggggctttt cttcaatcaa acaacgactg ggacgtaata agtcgtaacg tttcaccaaa
    12121 gtttccgact tcagcttttt ctttggtagc tgaaattcat attcttcaaa tggtgtaccc
    12181 ttttcgccat agatatgttc aatcaaatac atcagatcac tcggtgtgac tcgtctgact
    12241 ttaaatcttc gagccaattt actttccatt agtttttcaa gcttggtata tcggcgaatt
    12301 tcttcattgg ataaggaaac aaaatcgccc atcaaatgat gattcactcc atagacaaat
    12361 tcttgaaagc cggagaagaa agatttcttg agattcttta gattgacctc ttcatctgtc
    12421 gcaatcagtt tgaagccaat aaaaaagcga taatcaatct gactatcgcc aatcatggat
    12481 actaatgcct ctgtctgtaa atcaattcgt tgtttcgcta cttctgttaa tcgcccggta
    12541 atttcctttt ttgatcgttc ttgcgtggca cgaatactac tttctgtggc aatttgtaag
    12601 gcatgaatct tgccttcacg attttgcgca atcaattgac gaaagttatc gtggacttga
    12661 tatttctgtt caggtgacaa aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa
    12721 cattctccct cttgattaaa gacaagatta ttttcaatat acttaatcgg gtacatagtt
    12781 cacactcctt accagagtaa tgggttcgtc ccatcgtttc ttctgttctt tgactttctt
    12841 tcctgcgaaa gtgaccttaa atcgtaagtg atagctaata aaagatcgta aaaatcccaa
    12901 tggtttctta ccgtcaaacg ttttttgcga catgaaccaa gtaactgcca ccggtatacc
    12961 taagtatttt aaaaaggccc cctctatcat agatagtgga ggcacatggg caaatacgat
    13021 gacaaacagc aaggataaaa caaaccaagc catctggtta aaagtcacag gaaaaggcag
    13081 ttgaaagtca ttaattgcat aaatgacctt ttcaactgcc caaatactgg tgtagctttt
    13141 tattttcttc atcaaatcat tcctttaaaa agaaggatag tttagaaatc aactaccctt
    13201 caataatttt gttaatatgg atactcaaaa acacctcgac tcgttaccaa ataattgcct
    13261 ccaatctcca agtctctacc acacgcatca aaatcaatat acgtcttcaa gtgttctggt
    13321 acttctccaa aatcaccagc ttctacaatc aagaaatatg ctacatcact catatcatcg
    13381 caatctggat aatagataat atcatccaca tgctctacca tctcctcaaa agaattgaag
    13441 aaagcatgtt gaatctcaga agcaacttca ccaactggcg ttccttcaag ttcctccgct
    13501 aagccacaca accgattaat ttcctcaatt ggcgtatact catcaatctc aaagggaagt
    13561 tcataatcat gaattgcata ttcctcgtat tcgtcattta acccaattcg ttctttcact
    13621 tcatcaaaat cgacaggtgg ggtaaaccac gcccccacca attcaccttc gttatatttc
    13681 ccgaggttcg caatataaac acgcatctgt tccatcgtgc ttccccctta cgcaccgaca
    13741 attcggttaa acaaactgag taagacatcc ttcaccccag aagcattaaa gaccaatccg
    13801 accgcaatca aagcaatcac caaaaagcca atcagtttgg aaaactcccg cttaaatcct
    13861 aaaaacaaac caatgaccac aattgccagt aagactaagg attgggcatt ggttaaaaac
    13921 cattgatata tggataggta agttcctgat attataaata tctcaaactt ttccccccat
    13981 tcacacggag cgtgcgactt tcaccgcact ccgcgttcca tcgtgtttat attgaaaagt
    14041 atttatgtag tagacctata tcaagtttaa ttcttgattg ccagctaata ctctatattt
    14101 atttagtctt atgagctgga ctttatttag cttcaataga gctatatatt tttgtattgt
    14161 ttcttctgtt ttagcgtgta ttagacggtg taccgcctct tttaccaaaa taagattttt
    14221 ataatcgtca tttccaccag ctccagttgg tacaatatga tgacaatgta tttcattagc
    14281 ttgttgaaat tcttccagag ttatagcaca ttttccatgt tgagcagaaa atagcgaaat
    14341 tcgattgtct gcgtattcta ttgtgcgatt ttccaaagat aatttcatta actggtacat
    14401 cagtaattta tcaacactta gattggtgtg gataaatttt cttccctcta gagtgtagca
    14461 attcgctaaa tacacctttg ccattggttt tctgtgttgg atatatccaa cagggaaaat
    14521 aggttcgccc tcgccagcaa cataccgaag cattttagat ttaccatatc ttgttgcctc
    14581 ctttgtcgta agttgtctgc ctgtttttct caatctgccc cttttgtttg tacctaatct
    14641 gttagtgaag atagccatta cagctctatt cagtttcata caatccagat ttacattcgt
    14701 tgcaatctga taataatttt gcatacctaa taccattgaa ttataaagtc ttgtttcttc
    14761 taattccgtt ttcccttttg agggagaagc aattttcttt gcctgtgata ccagcttgtc
    14821 tttttggttt ttgaattgtt tatcacttat atgtgattta acaactaact tctttcttct
    14881 cggtatcatt ttaatcttga aaccaagaaa gtcggagtat ctttttctaa catttacaat
    14941 tcttgttttt tcttgtgata cttccaattt taatcgttgt tcaatccatt gagtgattgc
    15001 tattttggct ttttcagcac tttccttgta gcggcagaaa actctaaaat catctgcata
    15061 acgaaccata tacatttcct ttaactttgt tttcttcatt tctttgtaag cgttgctttt
    15121 acatgggcta ccgcttttat tgatatgaac cacatagttt tttgtaacag gattttcctg
    15181 ccattggctt tctacccaat ggtctaactc attaagaaca atgtttgcta gtagtgggga
    15241 tattattccc ccttgcggag ttcctttctc tggatatgtt attgtaccgt tagtcatttt
    15301 gattggagct tttaatatcc tttttaaaat gaatatcaag tgcttgtccc gtattcccat
    15361 agcccatatc tgttttatca gcttggaatg gttcacgttg tcaaagaaac ccttaatatc
    15421 aaattcaatc acataatgta gctggctgat ttgtaggcgg ttataggttg cttttattgc
    15481 attttcaact gacctattag gtctaaaacc atagctgttt tcactaaatt tagcttcgca
    15541 gattggttct aatacttgtt ttatacattg ttgtataagc ctgtcccaca tacaaggaat
    15601 acctagaggt ctggttttac catttggttt tggtatttct tttcgcctca ctggcttagg
    15661 tcgataccca tgtggactgc ctgcaacaat gtatcttact tttttgacaa ctgtttctgc
    15721 tggcaatttg ccaatatctt taattattgt accgtctgtt cctgctgttt tactgcctgc
    15781 atttgctttg atatttcgat atgccagtaa tatattttct ctcgatagga taatatccat
    15841 aagatgagtg aaaatatctc cattcttact tttggcatat aaatcatcaa aggtgttttg
    15901 catttcgtaa tattcagcgt gtcttaaatc ttctacacac aatgtttcat ttttattttt
    15961 aggcataagg cttcactcct ttacttgaaa ttgagtgagc ttttttatct tactcgtaat
    16021 ccttattgaa acaacataat acttttctaa tatatacacg acttgagggc attcctccat
    16081 tctcattaca agaatttctt aggttcgccc tctgcttttc agcattagtt caactgctta
    16141 ttgttcttcg tcagtttatt ctcttgatga gaacccaata cctttcctcg ttccgataat
    16201 cttatctata catatatatc cttaggtcac tagctataag cctgctggct tgataccgcc
    16261 tgtaacgtta tatggcattt cataacctat attcttactt tgccacacca gctacgattt
    16321 ctcgtacctt tgcatttcta caaagttcaa atttagaccc gtacattcgc tgtttcgtca
    16381 gttccttata gaacattcta accatagata ttttatagac ccccgacctg ttggatacgc
    16441 tacccacctc tggatagttt tcgatagaag atatatgttc atcaactatt acggtatctt
    16501 tcagccgtct ttaccgagct tcaaacattc tgagtttcct catacaatgc ttgtcggagg
    16561 ctcagggcag gcgtttcaga gcgttacttc atcattccac ctgtcagatt atcagttctc
    16621 ctaataacca aactttttcg ttcttttatt agtgccttag cttttcacta aggaacgagt
    16681 cgcacataaa ttttgtccga aattcatctt gtcactttcc tttcttttcc attgctcgat
    16741 ccgctgctgc acttgcattc atcaagcaca tcacagtaac accaagcata ctgccaccaa
    16801 ataccaaaaa aatatctttt acaattccca tgacgattcc tcttttcata taatcaaatt
    16861 ttcaattgcc atactttgtt gctccaaaat ctttttatgt cggtctgata agcgtgcatt
    16921 ctgtaccata tcctggatat aggttgtccg attcaatcga tccaactcct tagccatttt
    16981 ccacgtaggg gcgacttgtc ggcctagcca attcaaggtt cgttcaatgg tatacggttt
    17041 tggctcggaa gttaattgaa tctctttccg attccgtcca ataaaacgcc cccattgttc
    17101 attcaagggc cagttggttc gccttttacc ttctacttta tccgcaaatc gcaagtaacg
    17161 attgataatg gaaaacgtgg tgctctcaat gctacgacct tttaacaaat cttgaatcgc
    17221 atgaaaagca cggtcatttt tcagtcgaat ctcaaaacga tttttcgtct cagtttggtc
    17281 taatggaatc cctaacttga tatattgttc atagtctttt tcatacaaac agaaataaac
    17341 ctcactcttg agactgccga tatataaggt attgcccatg ccgtcctttt catcagcctt
    17401 taaaagttcc ccggaacgat aacttttaaa ggtacgaaac aaggagatac attcttcctt
    17461 ctgacatttt ttcgttagct caggtatatc caatagcccc actcgatcat tgattgccaa
    17521 gtccaagcgt ttcatcacgc caccggcctt tagacaatcc tcgaaaaagt cgtaccagct
    17581 acgcttttga gcgagtaaaa aagtttcaaa ttgtcggcaa cctcgcccct ttaattctaa
    17641 aagcacacct ttatcttctt catgagacaa catcaccaca atatcaccca tgacatactg
    17701 ttcttgatag gagtaaaagg cataatcttc atggagcata tactcaaatt tcaaatggag
    17761 aatttcttga ataatttttc gtgcatccgt tgtgggaaag cgaatcctta catagtcaaa
    17821 cagtaacgtc aaaggcaaat cgggattaaa gctttccagt tggtcaaaaa ttttcctcat
    17881 ggtttttata gatgcggtta cgccaccatt ttctatctga tttaagtacg gccgactaag
    17941 tccacaagcc gtagccaatt tattttgtga aacgccgtat tcgttgcgtc ggtcttttaa
    18001 tagccgataa tctaaattcc gttgtgccaa gttctcacct ccaaattcaa ggtgttaact
    18061 ttttcaaatg agttaacacc tatacaaatt acttactatc tttgatacaa caagcattct
    18121 taatggttgc ttacttattt tttgtgtttt tgtacccccc tgttagatac gggggtgaga
    18181 catcatggtc ggctaacgcc gtccatgaac aggcacagca ccacggagga tttctgcttc
    18241 gcgaatcctc cgcggtcagc gcctaacagt cagttgtgtt ggtttaggtt actcatctgt
    18301 aataccattt acttccaact ctgctttcat ttctaacaag tattgacgaa tatctggttc
    18361 ttttgcttgg tccgcaatcc gctcaaattc tgcaattact tgatttattt caattcgagc
    18421 atccgtctcc tcattcatta aatatggatc gccagcgact ttgacaccat tcttgcgaat
    18481 atcaaatttt aaccgtcgca attgttcttc taattctggg tctgactgaa tctttccaat
    18541 cacccaataa atttctccaa tcaactcatc ataaaccatg tacatccaac tcttttaatc
    18601 ttctggcaat atcaaaaatg accggaactg ttacagagtt tccggcttgc ttatacaact
    18661 gactatctga attgacttga gaatctcgat caaaagccca atcgggaaag ccctgaagtc
    18721 gccaacactc tctaggtgtt agcttccgaa tttgatactg atccgtgacg atcgcttgtt
    18781 ggtctcctgt tagtaaggta tgaacactct gttttcccag acgacctcgg cgacttgtgg
    18841 aattaggaaa agccagattc acactgtctt ctggcttcac ctcttgatat tctgtttcat
    18901 tttttcgaat catgattttt gtaccacctt gtttcgttga caaggtgggg gcggttccag
    18961 taacttcata aactcgatta ccttgttcaa actttcccgg taatagtcct gctacagaga
    19021 ttccatccct tgctggcagt gcgattttga ttccttcatc acctgttaag gttggcgcta
    19081 cgccatctga accatagact tgtccaccca ttcctcgatt ggaaggattg atatttacca
    19141 aatttctgat gttttgatca gatgttgaag acggtccaat gataggaaat acttgttcgg
    19201 tacatgcgtc tcgagaatgt ccgataagga atatccgctc ccggctttgg ggaatgtagg
    19261 cctttgagtt aagcacctac cactccacgt cataccccaa tccatccagc gttcggagga
    19321 tcgtctcgaa cgtagcccct ccttcgtgat tgagcaatcc tcggacgttc tcaaggaata
    19381 aaagcttagg tcggagaaga gtggtgaacc gagcgatttc aaagaataaa gtacctcgag
    19441 tatcggaaaa tctttgccgc tttcccgcaa ttgaaaaagc ttggcacgga aatccgccgc
    19501 aaaggatgtc cactggtccg agggattgaa taaactcatc tgatacactt gtgatgtcat
    19561 gcatttccac ctccttactt gtatcatgaa ttgctttata actacgtcta gcaaatttat
    19621 caatttcaca aaaaccgata caatgatggc tcgcctgttc catacctaaa cgaaagccac
    19681 caataccggc gaataaatct aaaaatctca tacattacct cattcattgc aaagaaaaaa
    19741 gccaagaaca ttttgctctt ggctcataca ttattaattt gtcctactat tttttattta
    19801 atccaactaa tttccgttgt ttttcattgg cctgatcggc tacatattta atcaccgttt
    19861 tgaactcttt tgcagtagca ttttccgata aatccgcaat gctcttattg tatttcttta
    19921 caattttcgt tacttgttct ttctcagtca tgtaattgaa cctccaaatc tttcctacta
    19981 taccctagtg gtaaaaataa ttctagatta tccgtagcat aatgaacttc ggaaatatta
    20041 ttcgtataat cttttattat gatatgaagc aatacatctg gatgtttact ctttactttt
    20101 gcaatgttct ctcttgattt gaaaaaagcg ttaataaatg cctttttcgg cacatgacgt
    20161 ccctctgcaa tttcacgttc ttgtgtaaat ttccaagcga ttttagggtc ttgataaaca
    20221 tagaaaatcg tcactcgaaa atgttttttc aacgcccgcg agatattttt ttcagcactt
    20281 aatattgcaa aagttgcatc cagaataaaa gaatagccat gatctaagac atatctaaat
    20341 gcctgctcta ccaaccaaga agatgctttt tgaaagagac tagagttttt tccattataa
    20401 tcaggaaact gggtgcgaaa gtaatccgca tcaataatca catggttatc ataattctca
    20461 gctaatccaa tggcaacctc tgattttcca gccccaggac tccctgccat aaaaactgcg
    20521 tctcgtccaa cttcggggga tttccctttg ataatataag aaataaactt atccttattt
    20581 gcttttgcat actctaaata agaatcatta ctttccatat taattatccc ctttcttcct
    20641 actatataaa agtattatac gttacttgaa gtataaagtc agtattgaag atcactcaat
    20701 cattcatctt ttctaattta ctaattgaat ctaagaaatc ataccctttt ggtacaagtg
    20761 gcgtataaaa ctcactaatc acactgccac ctgtatccac ataacctcga cctttgatgt
    20821 gcttcataaa gaagtttttt tcgacctccc caaacatcat ggaatatccg agttcactca
    20881 tacgacccaa agccacgcga aagttgaatt gatcgcggat tccgtctcct aaatatttcg
    20941 catccggcct ttggcaagcc aaaatcagaa agaaaccaga ttgacgaccc aacatcacga
    21001 tttgtttgag cttattcaaa atcaccgcac tttctttcgt cgttaacatc tccatataag
    21061 ccacgtattc atcaaagatt aaaaagttgg gtggaagtcc aagataggca taattctctc
    21121 ctgttttgta gttagacatt tctttcattg ccttgctacg agccatcata cgttcataga
    21181 aatcttccac acaagcagaa atttcttcct tttgtgaata aacatgaggc atcaccgtac
    21241 ctaaatcagc taaatctgca tttttgggat cgaggataaa caattctgca tctgacttca
    21301 atagtgcttc gataatggtg aggaggaaat aggtcttccc tccacccgtg ccaccagcga
    21361 ttaacatatg gggtaaagaa tcataagccc atacttgatt tttcatcaat cgcagagtcc
    21421 cattttctgc cactacttcg tctatcccaa ttcgattggc gatcatatca tacagtaaag
    21481 tatactccac ataggagtct ttgagttcct tttctactaa ctcacaatac agtccgcttt
    21541 ctaacttctt ttccaatttt aaaagctgat cttggtattt cccaagtgaa atttgaacgc
    21601 gaatagacag taatccatct ttcattcgat aacagatttt ggggaagtaa ctgatagtct
    21661 ctctggttcg gctactattc aaatctttga aaaagccttc actttgagtt tgtttcactt
    21721 cataccaatg gttctctaga atcatccgag ctagcttttg tcgatgccac aagcttcgcc
    21781 aatgatcaag aaaaaagtga atataaagca atatcatagc tcctgaaaaa gcgagacttc
    21841 caagcagaca tactaaatct tttatagacc aaacgatttc aatagtagaa agtgatagct
    21901 tagaaatatc aaattgtcta aactcttgaa aataaaagac gctcatccaa ccgatgaaca
    21961 gagcgagtag ccagccaaga acaaagtgat aaaccaagtg ctgatctcct gcgcgtatgc
    22021 gatgtccttt gtacattttg atggtttcca cctccaatcg aaaaagccca ataggatttc
    22081 attaggcaat ttcaaaaagt tatgctagtc atttactgct acgaggaggg gagttgtttc
    22141 gtttcaacca attgatttaa aaagccttca atagcatccg gcatctccac accaatttct
    22201 tccaaaatgg attctgcctg ccgacataag ttttcattta cttcgacaac tataaagaaa
    22261 gactcttctt ctcctaacca gtctagtaca tcagagtctt cttcactgtc gctattttct
    22321 ccaagtaaac cttgatgtat aatcagaaac attttttcat cagaggttaa tgctttaacg
    22381 agttgttcct cagtcagctc gccttttcca tattgatcga gacaatctaa aatatgttca
    22441 acattaattt ccataatctt cacctatttc tttctttcat tttggttcgt aggatttcct
    22501 tgattcggct gccgcttcaa gactaaatca tccgccttaa tgaaccaatt aacggtagtt
    22561 ccttctcgaa aaacataatt ggcaacggta tccacgattg gattgaccaa ctctacctct
    22621 gcatcataag caaactcgcg taagggtaca ttagctggga tactaacttg aatcattcgc
    22681 ccttgtgcag aggattttaa attataggtt cgttctttta cctcattacc aattgttcca
    22741 tcctcatttt gcttccgtac ttctcggcgt aatgcggaaa aacgcaactt cccaaatgtt
    22801 aaacctgaat ctacgacaat accttctgtt aatctcatct tcttttcctc ctatgctttc
    22861 actaaatcat caacatacaa cacgtaatca gtaaacccac gattctcaat ctcatagcct
    22921 ttggcaacca aatggggatt cacggctttt agcggttggt cttgatcaaa atccttttca
    22981 ccggcttccg cagctactac cacctcaata tcatcggccc gttgcttact ggaatacaaa
    23041 tgataactgc gaccaatcac cgtagtattc cgtccatatc cttctgttat aatttctcct
    23101 tcaccggcat aacttaactt tccaaaggtt tcagccatat ctggcacgac aaatttcaat
    23161 tccatcttct tttcctcttt tcattgttag ttttggatac aaaaatagcg cgactacttt
    23221 tttaggaggc ggtcgcgcac gttggttctt aggatttact tgtatcatct catcacctta
    23281 gttccggcga ttccagaaat aatagcccgc cgtcgcaaaa atcaaagtaa aaccaatgaa
    23341 cagtaaaaca ttggaatttt tctccccggt ttgagggaag gtcttcactg tgggactaga
    23401 ttcttgagag ccacttggaa cagttggtgt ttccggttgt ttcggcgtac ttagtatggt
    23461 tggaacttct tttggtgtta aggtttgaga tttttctttc aaatcttcat tgtgttttcc
    23521 attgatattc ccatctttat cgtaattgat ttccgcaaaa gtaaacttgg ttccttctgg
    23581 atattttccg gtatctactt ttttcgcaag cacagttttg gtgaattctt tatcatttac
    23641 ttcataatca atcttgccag atttccaaat ttctttgttt gtaccatccg gtaataaagc
    23701 atacagaatt gtttcgaaag cttcttttga gccatccagt acatcatgag taatcgacac
    23761 atcatcaaac atatccacca catcaccatg agtaaaggtc tgagaaccgt tttctaggtg
    23821 ggcctttgtt tggatagaaa catggtgttc cactgtacaa ctgacagttt gggcttggtt
    23881 attcaagcta acatccttcg ctactggctt atcgcctgct tcaaaagctt ccttattttc
    23941 atacacatag ttgaataaga caatagtttt atttaaaact tgttcggcgg ttaatttatg
    24001 ctgaattttc caagttcctg ttttctcttt gttcgccaac gttgcgcttg tttctgcaat
    24061 caccactggt ttggcttttt catctttttc ttgggcagct tttgtcgcat ccacatcaat
    24121 ggcttgcgct accacatacc agtcttcttt gatttcatgt agattgtaac tcaatttatc
    24181 aactagctcg gtagaatcca gagaagttaa ttctttgttt ccgtctttcc aagtcgcaag
    24241 agaagttaaa ctatcttctt cctcaggcaa atcatagagc attaaacggt tcaaactaac
    24301 agaaaatgct ttgttcgtca gcttctcgta aggaacggtc accgttttaa ttggttgttt
    24361 ttggtcttgt tcggtaatgg taaagacata ctcactcttc acaaagtctt ctttgttttc
    24421 cttaaaagta gaacggattt ctaatggcgt aatcttttga aaaccttctg gggcttctac
    24481 ttcttcaagt aaataatccc cataaggaag attttcaaac ttgccataac catcaaaccc
    24541 taattgctcg ttataagctg tggtcgcttc atcttcggca cctgtgattt cattggtccc
    24601 ttccaatggc gacactttaa aggtcagatc attgaagcca gtttcggtag tgcccgcagc
    24661 tgatccagca aatttaaaga aatcaaagcc aaagcgaatc acttgttctt ttgccgtaac
    24721 atttcgggta atcacggcat ttttttcgct atcatccacc tttttgatgg atacaggata
    24781 ctttgtttca tcaaggctat atccttcggg cgcttttgtt tcttgccaat aatactcgtt
    24841 gatagctaaa tgtttaacgg caacttggtt cttttcgtct aaagtcaaag tgaccgtttc
    24901 atcagaacct ttcgttccct tcgctaactc tggtttaaaa gcctcactcc atttaacagc
    24961 ttttccatcc ttagcagtaa acaaggtgta ttcagcaccc tcaaagactg ccttaccttg
    25021 tgctttatcc ccagtatctt tgtcttcttt cgtcaaagtg gtttcgccag tgacttcttg
    25081 attttgccct tttacgttac tggtaacagg ggctacggtt tgattggcat acttcaattc
    25141 gacttttaca ggtttgaacg tattcacgaa cccatgacta gctttagttt cggtcacata
    25201 ataggttccc aattccaacg cattagcaat ctcttttggt gtttccgcat gaccgttttc
    25261 atccgtggtc atttcttgaa cgatttcacc ggttggactg tctttgcgaa tggcaaacgt
    25321 attaccagct aaggaataat tgtcattcca aagatcactc cctgtttcaa ctccgctttt
    25381 atccagaatg atttgccctt tttcccgtgc gttttttgaa gtcacgtaaa tggtttcgcc
    25441 tgctttaatg gtggtagtca tgggggtggt atcaatcgta taaggcgccg gtactgattt
    25501 ttcggtaatg gttacttttg taccatgagg aatctcatcc aatgtagcaa tgccttcttt
    25561 gtcagtagtc acgtcttttg caggtaaagt ttttccaaag tctaaatgga aaaccgttcc
    25621 tggtacaaca tcacccgatt ctttatcgac ttttttgatc tttaaagaac cctccgtttc
    25681 cacatcaatt ttgattgtgt aggtattagg tttatcaatc gccccggcca ttaaagtctg
    25741 ttggccagcc tttttatagg cgacaggtgt tccggtacca gcagatttct taagcgtcag
    25801 cacaccactt tcttttgaat tggcatttgg ggtgattatc aactgattac cattcacacg
    25861 ataatcaatg tttgcggcat tttccacaac ctcatcgaac tctgacaaat tcagattatt
    25921 cgtatccgtc acagtagtcg attgccctaa tactgtttta gccgtactat tatggaaggt
    25981 tggtttcttc tgataatcgt caatggcttg atttattttt gcctcaatgg ctgcgatatt
    26041 tacggcacta ccatctgatc gttttattga gtgaagggta tacccattaa cttcttgcca
    26101 gatcatcttc tgtgccacaa tatgagtatc tgcatctgta cctacttttt tccataaaac
    26161 ggaaactaat ttggctttat cggacatatc tggtaacggg tttttctcat agccaggagt
    26221 gaactcattg taaatcggga ctcctggttc aatacagaaa atatcttgtt taaagccgtt
    26281 gtacactgca taatgcggaa cagtgacttc tgacattgga cgaccatcac taaaagttcc
    26341 ttcaacagca tattgatgtg ctaaatctag ttctacggtt accgtttgtg gatgggtaat
    26401 ttcatcagca aaagtaatcg taggagcaat tgctccaaca attgcttgaa cagccaaact
    26461 caaaatagtt aatacacata atttcttttt ttgtttttta tacatgaaat gttctccttt
    26521 caaattaagc acaaaaaaat aagatgctcg cttgaacatc tttgtttata aagtcttttt
    26581 aaaattttta ttgactgaat agaatggatt tgagaatgaa tgagcctaaa aatttaatct
    26641 aaaatacgaa atatactgcc tttaaattag aacacatttt agaacacaaa catttcaaat
    26701 ttaatcaaat ataataaaat atatttacgg gaaaaccttt ataaatcaag gtttttcaaa
    26761 cttattcaat gatacgaaga aaaaaccagg tcttaagaaa gcccgtaaag cttcacagtt
    26821 ctcaaaacgt taattgcgat tatcgttcga gttcacgagt tacgagaatc gtactgggcg
    26881 aagcaatgct gagcgcagtt accttaatcc taacttttct ggcactttcc acaatttgga
    26941 aagtgccttt tttgtgtttt tagctatgtt aataggtagg attcttggtc tataatatca
    27001 ttcgatctat aaacaaacgg gaaaaagata aagtatcatc aaacaatatt atttatccgc
    27061 ttctgaggtt gtgacagaag tggtcagctt caagaaataa gaaggaattt acaaaaattg
    27121 cttctcatgt ttgataaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
    27181 tattcggaag aagggattgt gaaaagtctt ttgtcataac ctcttatttg gtagctaatt
    27241 gtaaatccca aacggcatgt attatttttc ataagtccgt ttttaaggat taatgttttc
    27301 ttttgctttt tatatggtct ggcttcagcg agaagttcca acgcatatac aaggacgact
    27361 tatgtcaacg ataacaacga ttaccatggt ggccagtcca cttggattag cactgtttgg
    27421 tgcgctcttt gattggaaga caacgcaacc acgtctgcga gacagtctaa tttttctggg
    27481 agggagtctt gctatagtta tccttataac cggtctggta aaattactca agcttgatct
    27541 gaaagaagca aaaattttca gtgaagagga atacagagaa acgactttgg tcgaaaagaa
    27601 agaatcagga aactgataca agcgtataac aaactgcctg ataataggaa agacacgtat
    27661 tgtccgattg ttttttttga cattcaattg gaaaatttac actttcttta tacatttctg
    27721 gctgctttct ttatacccgt caactatact tcactcatca ataagaaaga aggataagta
    27781 tggattttgg aaatgaaacg gtcttgaaag caactggtat aaccaaaaaa tacggtgctg
    27841 ccaaagcatt agataaagta tcaatcgaaa tcaaacgtgg aatgatctat ggattgattg
    27901 gggaaaatgg agcgggtaag tctaccttta tgagaactat tatgggtttg atctcgattg
    27961 atgaaggttc gatcgaatta tttggaacga cggatttgca ggcagcaaga cgacgtatgg
    28021 gtcaatcgat tgaaacccct gctctttatc cagaattaac tgcaagagat aacctacgta
    28081 tccaagcagc gaacggcggt gtgtcggata gagaaatcga agacctgctg aaaatgatgc
    28141 gattggaaaa tacagggaaa aagaaagcaa agaatttctc tttaggtatg cgccagcgtt
    28201 tagctattgc aaatgcactg atcacgaatc cagaattttt gattttggat gaaccgacga
    28261 atgggatgga tccagcaggg atggcagaaa tgcgagaaat catccaacgt ttggtaaaag
    28321 aacggggcat caccgtcttg ctttctagcc acttgctaga tgaattgtca cagatcgcca
    28381 ctcactatgg tattttgcat gaaggacact tgatcaaaga attatcgaaa gaagaattgg
    28441 cacaagaatc tcgtcaattt ataaaaattg acacttcggc gacggaacaa gcagtaacgg
    28501 ttctcgactc tcttggttat agggattatt ttgtacaatc tagccgtgtt atccagcttt
    28561 ttgaaggaat tgaccaagta gcagccatca atcaagcatt ggtagaagca aaagttcctg
    28621 tggatggtat tcacctagtt gggcaaaaac tggaagatta tttcttacaa ttaactggag
    28681 gcaatccaca tgtttaatct attaactgct gaaaaaataa aagtatggaa gagccgtaaa
    28741 atgtgggtct cactaggact gatggtcgta cttccgcttt tttattcttg gaacgaatgg
    28801 tatatgcata caaaatacgg caatgaatta aaccaagcaa cggatacagt catcaacggt
    28861 gcaacaggga ttttaatggt agaaaaaatg gctgcatgga ttcttctggc tttcgcagca
    28921 tttgcgtgct tttatattgg ggaagaattt caaaacggta cgattcgcaa tacgttatct
    28981 ttaggacgga atcgaatgac ttattatgtg tcgaaacttc tcgttacgct tttgatcaca
    29041 acacttggcg tggttttgat cacgggacta gccatgcttg cttacacact tgctttcgga
    29101 tttggtgaag ttgagggaat caaaaactat ggaaattatg ttctaaaagt atttcctgta
    29161 ttgcttctgc tgacattagg gacattatct gttccagtgg ctttgacatt catcactaga
    29221 agtaccagtg tcagtttgct tctttctttt ctttatatca tgggaacggc cttcattccc
    29281 ggtgttttcg caaaaatcaa aggcttggaa tttctaacgg agtggtttac ggaaacgtgg
    29341 ctcatgtata cggactttgc gcaacaagca acttattcgc aagcaccgaa aatgattctg
    29401 gtcagtctag taacaatcgt ggtatcatca gctctaggaa tgtttatttt ccaaaaatct
    29461 gacattaagt aggtggtcct gctggaaact attttgatta tcgaagacga tgaagctgtt
    29521 cattccttac tggaggaggt tcttgaacag agatacaaac ttcttgatgc ttattcaggt
    29581 acggaaggaa aattattgtt aagcacatat cctgtcgatt tgatccttct agatctcatg
    29641 cttccagggc tttcaggtga agcattgtta gcagaaatcc gacagacatc gaatgttccg
    29701 atcatcgttc tttcagcaaa aagcgatcaa agagataaag tttccttgtt ggcagcagga
    29761 gcggatgact atgtgacgaa accatttgat atcgaagaac ttttgttacg aatcggcatc
    29821 caacttcgtc atcagacttc tgtccaagta aaagatctgt cgcaacgcat ctattacaag
    29881 gaaatcttac tggacaaaga gtcgagggat gtaaatgtga atggtgtggc tgtccatttg
    29941 acaggaagag aattcgatct gctgaagctt tttttagaac atccgaaaaa agtattttcg
    30001 cgagcaaatc tttatgaaac agtttggcaa gaaccatatt ttgatagtga gaaaacagtg
    30061 aatatgcata tttccaatct tcgagggaag ctagcaaagg cacatgctgt gtatattcat
    30121 acggtgtggg gagtggggtt tagatttgat taaatggctg ttgcttctcg ttgttatctt
    30181 tatgggaagt tttattttct tcctgcaaag ggaaatcaag agattaacta agaaaatcgc
    30241 cactctttct gaacgacaag aatatggtgg tcgtctttca gtcgaatttc gagaaaagaa
    30301 tctaatggac ctagtagatg cactgaatac atggttggat gaaaatgaac aaaaaggaca
    30361 agcatttcaa gaaatggaag aaaacatcag actatcgatc gtcggccttt ctcatgatct
    30421 cagaactcct ttgactgcta ttaacggtta tgtacaatta ctggaacagg tagaggatac
    30481 ggagaaacgg gagaagtatt taacgatcat caaacaatct gttgcaagat tactcagtat
    30541 gacggatcag ttttatgatc ttgctcgaat agagacgaag caaaaagaaa tggatctcca
    30601 tacgctttct ttttctaaag aggtggaaga agtatttttc tctttttatg aacaattttc
    30661 tgagaaaaat atcgaggtag cgttcgctga acactcgata aatacaactg tattagcaga
    30721 tccgctcatg ttgctgcgag tgctgcaaaa tatcatacag aatttattgc gttatgcaga
    30781 tggacaagca aaggtgacat tttcaaaaga ggatccattc attcgattaa cagtggccaa
    30841 tcctatcaaa ccaggaacgc aaacctctgt tgaaaaagtc ttccagcgtt tttataccga
    30901 aaataccagc cgcacaaata cggaatctag cggaatcggg ctttacctgt caaaaaaatt
    30961 ggtggaagga atgggaggag agatgaccgc tagattagac gggggcatct tctccatctc
    31021 cgtcaagttg cggagggttt aattatacaa gattagtgag aactagtaga aaaatgtatt
    31081 atcttatata ta
//
LOCUS       DAZAFF010000026        31089 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000026 DAZAFF010000000
VERSION     DAZAFF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.026"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..729)
                     /locus_tag="ACG9BB_001128"
     CDS             complement(19..729)
                     /locus_tag="ACG9BB_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFN2837085.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="ACG9BB_001129"
     CDS             complement(726..2198)
                     /locus_tag="ACG9BB_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFN2837086.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="ACG9BB_001130"
     CDS             2336..2809
                     /locus_tag="ACG9BB_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN2837087.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="ACG9BB_001131"
     CDS             complement(2962..3513)
                     /locus_tag="ACG9BB_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN2837088.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="ACG9BB_001132"
     CDS             3618..4148
                     /locus_tag="ACG9BB_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFN2837089.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="ACG9BB_001133"
     CDS             complement(4631..5074)
                     /locus_tag="ACG9BB_001133"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2837090.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="ACG9BB_001134"
     CDS             complement(5090..6250)
                     /locus_tag="ACG9BB_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN2837091.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="ACG9BB_001135"
     CDS             6757..8457
                     /locus_tag="ACG9BB_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837092.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DVPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="ACG9BB_001136"
     CDS             complement(8559..9392)
                     /locus_tag="ACG9BB_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFN2837093.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="ACG9BB_001137"
     CDS             complement(9696..11861)
                     /locus_tag="ACG9BB_001137"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFN2837094.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="ACG9BB_001138"
     CDS             12121..14415
                     /locus_tag="ACG9BB_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFN2837095.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="ACG9BB_001139"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="ACG9BB_001139"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFN2837096.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="ACG9BB_001140"
     CDS             15066..16073
                     /locus_tag="ACG9BB_001140"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN2837097.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="ACG9BB_001141"
     CDS             16095..17114
                     /locus_tag="ACG9BB_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2837098.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="ACG9BB_001142"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="ACG9BB_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFN2837099.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /gene="tyrP"
                     /locus_tag="ACG9BB_001143"
     CDS             complement(18871..20292)
                     /gene="tyrP"
                     /locus_tag="ACG9BB_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFN2837100.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="ACG9BB_001144"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="ACG9BB_001144"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFN2837101.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22703..23959)
                     /gene="tyrS"
                     /locus_tag="ACG9BB_001145"
     CDS             complement(22703..23959)
                     /gene="tyrS"
                     /locus_tag="ACG9BB_001145"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFN2837102.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24071..24276)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24539..25840
                     /locus_tag="ACG9BB_001146"
     CDS             24539..25840
                     /locus_tag="ACG9BB_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN2837103.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25909..26508)
                     /locus_tag="ACG9BB_001147"
     CDS             complement(25909..26508)
                     /locus_tag="ACG9BB_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2837104.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            26762..27643
                     /locus_tag="ACG9BB_001148"
     CDS             26762..27643
                     /locus_tag="ACG9BB_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN2837105.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            27656..29266
                     /locus_tag="ACG9BB_001149"
     CDS             27656..29266
                     /locus_tag="ACG9BB_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2837106.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(29343..30002)
                     /locus_tag="ACG9BB_001150"
     CDS             complement(29343..30002)
                     /locus_tag="ACG9BB_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN2837107.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(30285..30950)
                     /locus_tag="ACG9BB_001151"
     CDS             complement(30285..30950)
                     /locus_tag="ACG9BB_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFN2837108.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         9296 a         6340 c         5644 g         9809 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgtcccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    22621 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    22681 caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    22741 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    22801 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    22861 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    22921 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    22981 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    23041 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    23101 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    23161 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    23221 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    23281 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    23341 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    23401 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    23461 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    23521 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    23581 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    23641 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    23701 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    23761 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    23821 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    23881 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    23941 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    24001 tgctagcata tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    24061 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    24121 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    24181 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    24241 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    24301 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    24361 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    24421 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    24481 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    24541 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    24601 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    24661 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    24721 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    24781 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    24841 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    24901 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    24961 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    25021 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    25081 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    25141 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    25201 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    25261 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    25321 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    25381 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    25441 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    25501 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    25561 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    25621 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    25681 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    25741 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    25801 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    25861 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    25921 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    25981 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    26041 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    26101 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    26161 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    26221 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    26281 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    26341 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    26401 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    26461 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    26521 ccgtaatttt aaacttttat aatatatata gttcaaattt attctgaggt ttattataac
    26581 tatctattga gaaagtatca agctagcaat tccttgttta tgaattcatt gacagaaatt
    26641 tataaaaaga atattatgta ctaaaataat aaaaaaagtt ttataaaaag gtataaaacc
    26701 tttttattaa actatttaat cttgtttagt atatattttt agaaaaagga gtggggtttt
    26761 tatggcagaa atcgtcaaag tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt
    26821 aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg
    26881 ttctggaaaa tccacgacga ttcggacact tttagggatt ataaaaaaag acggcggaac
    26941 ggcaacgatt tttgggcaag atgtatggga agatagttta tctatccatg aacgtctttc
    27001 ttatgttcca ggcgatatct ctttgtgggg aaatttgact ggaggagcga tcatcgatct
    27061 cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt
    27121 ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg
    27181 cttgattgct gctttggctg ttgattcgga tctctattta tttgatgagc ctacttctgg
    27241 tttagatcct ttgatggaaa cgattttcca agaagaagtt gaaaaattga aagaacaggg
    27301 caaatccatc ctactttctt ctcatattct cagtgaagtc gagcgactag cggataaagt
    27361 cgtgatcata cgcgaaggaa cactcgatga agaaggaaca ctcgatgaat tgcgtcattt
    27421 gactcgttcg accatcacta tagaaacaga agcagagaat agtcacttag cacaattgaa
    27481 cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc
    27541 tttcgatgtg attttagctg aagcgtccaa attaggtgtg aaaaaatttg aatctattcc
    27601 accgactctt gaggatttat tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac
    27661 tgataaattt tcacgctggt ccaccctttt ttctcaaaac cttagccgtg actggaaaaa
    27721 gatcctgata tggctagtcg gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga
    27781 agaattgact tccggacaag gattagctgg attatacgaa accttgcaaa acccggcaat
    27841 gattgcaatg gtcggaccaa ctccagcttc gaatgctgct gagtatactt taggagcgat
    27901 gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct gctgtgatct ctgttttaca
    27961 cgtggtgagc caaacgagaa aaaaagaaga acttggtctg attgaactta ttcgttcctt
    28021 tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct gaaagtattc tgatcaattt
    28081 gcttttagca atatcgatca gctgtacgat gatttttttc caagcggatt cgatcactgc
    28141 agaaggtgct tggctttttg ggctatctgt aggtagtatg gggattttgg ggagtgccgt
    28201 tgcactgttt gctgcacaac tgatgccaag ttcagccagt tcgaatggtc tgtctttagc
    28261 gtttttaggt ggactttacc ttctacgagc aataacagat gtagctgatt cctccctttc
    28321 gatgtggaat cctttaggct ggtcttattt gacctatcca tttacaacta acaactggat
    28381 tcctttgctt tatttgtttt tgttcagtct aatcttgata atcagttcat ttgttttaga
    28441 ggaacatcga gatttgaaca ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa
    28501 acgttctctt ttatccttgc gtggtttatt attctatcta aataaaggga tgacaatcag
    28561 ttggcttatc acttttggtt tgatgggggc ggcatacggc tctatctacg ggaatatgca
    28621 acagttttta gaaagtaacg acttaatgaa acagatgttt atccaatctg gcgtctcaat
    28681 tgaaaaaaac tttacaagca cgatcatggt agtcttgatt tgtctagctg ctattttacc
    28741 aattgctagc atcaatcgtt tatttacaga agaaacccac ggacgtatga atcagctgtt
    28801 cgcaacaaaa atttctagga accaacttta ttggaccaat atgttactag ctattctatt
    28861 tgctatattg gcaatcctga ttagttctgg tgcactaggg ctttctgctt tggcttcgat
    28921 gaaggatgcg aaagacatgg atctattgat gtttcttaaa gccgggttca atttcctgcc
    28981 tgccgtttta gtgtttatcg gaattagtgg ctttctattc ggatggcttc ctcgaatcgg
    29041 taaacttgct tatatctact tagcttactc ctttgcactc aattactttg gcggaatttt
    29101 ggatttacca gaatggtttt ccaaaacagc tgtccctagc tgggttccac ggatgccaaa
    29161 cgattctttt gatctttccg tgttccttac gttatcatgt atcagccttg cactgttcgt
    29221 ccttggttct attggttata ggcgaagaga cttactagaa ggataataaa aaagcatgta
    29281 caatttcatg ctagcctcca aatctgggtc aaaaatccaa catttggagg tcattttttt
    29341 atttacaagt cttctaactg ctctttatca atgatccaga cttctcgatt ctttcttttt
    29401 acataaccat tctcctctaa taatttaaat tttctagata aagtttcggg ggtcgtaccg
    29461 ataaatgcag caagttcttt cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct
    29521 ttagctaagt caagcagata gagagcgact cgttcttcta ctttttccat catcaaaaaa
    29581 gttgtctgct gctgtacttt tgttaatttc tcaacgttga ttgctaaaag tttcagacta
    29641 agggcgggat aatgcagtaa gagttccgag aaatcttttt gtctcaatat gcaaatttcc
    29701 gttttttcta gtgcttcgcc aaataaagac tcatttggaa cgcctagcaa ctggctctct
    29761 ccctcatatc cgcccggctc tattactcgt aaaagctgtt cctttccgct agcagataat
    29821 tgatacactt tcatactgcc ggaggccacg atcactaatt gaggttctcc attcggcgca
    29881 agaatctgtt ctcctttttc cacatagtga tgtttgacaa gtgcgtgtac tttttcttga
    29941 tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa cacataaatg atcatgactc
    30001 ataatctctc ctccttctaa acagctattt ctaataaaga aacataactt cccttattcc
    30061 aactgataat tttcttttat catagcataa acgtcaagcg aaccagatac cagcaaatag
    30121 agtggatttc tttttgtccc ttgctttatt ttccttcttt tgagacctta agtaattgat
    30181 tcctatcaat tttttaaata tttacttttt gaataatcaa aaacaaacct tcactcgcaa
    30241 gccgagagta aggtttgttt ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc
    30301 gcacctagct tttgatatag tggtgctagt tcttgtaagg tttcagcggt tatttgctca
    30361 acgtgttcga tttcttgcgg atagaagaat agtttaccca tttgaaaggt tcggtctctc
    30421 atcgcttttt tcaactcttc ttgtttcttt gtatcggaca aatagtcaga caaagctaag
    30481 tggtcgtcaa gttcatgttc tggttgcggc catatgtaat aatccttgat atcgactgtt
    30541 ttgctccact ctttcagtat cgcaagcaac tggttatacg ctgcaatgct accatatcgt
    30601 tcttcgcttt tataatgttg gtacatcaaa tagatttggt attgtttttg attcaacaaa
    30661 actgctagac aggcattctc gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga
    30721 ctgcgtaaat tccagccatt cgtccagctt tcgattttag gtttgctcat gccgtacata
    30781 tcaggcaaaa gtgcagctgt ctggagctga atctctttcc atttttgcca gtgttcttta
    30841 tattctttct tgatttgttc aatcttatct gttggatggc tctctttgag ctgtttgaat
    30901 gcgtagattg gtcgatcaaa caactgataa tctgattttc caagttccat tgtttcgcct
    30961 ctttccgtta tcttttgtat tctacctgaa ttttgcgata tggacaaata gaggttgtga
    31021 aaaagctgtt ctgcatcaag tcataagaac agccaaacaa aaaactgcga tgagcatcac
    31081 cgcagtttt
//
LOCUS       DAZAFF010000027        30899 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000027 DAZAFF010000000
VERSION     DAZAFF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.027"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG9BB_001152"
     CDS             complement(296..1024)
                     /locus_tag="ACG9BB_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2837109.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG9BB_001153"
     CDS             complement(1002..2576)
                     /locus_tag="ACG9BB_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN2837110.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG9BB_001154"
     CDS             2805..3743
                     /locus_tag="ACG9BB_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2837111.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG9BB_001155"
     CDS             3755..4666
                     /locus_tag="ACG9BB_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2837112.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG9BB_001156"
     CDS             4690..6162
                     /locus_tag="ACG9BB_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN2837113.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACG9BB_001157"
     CDS             6213..6824
                     /locus_tag="ACG9BB_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFN2837114.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACG9BB_001158"
     CDS             6854..8767
                     /locus_tag="ACG9BB_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFN2837115.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACG9BB_001159"
     CDS             8895..11876
                     /locus_tag="ACG9BB_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFN2837116.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACG9BB_001160"
     CDS             11873..13534
                     /locus_tag="ACG9BB_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFN2837117.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACG9BB_001161"
     CDS             13574..14506
                     /locus_tag="ACG9BB_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFN2837118.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14898..16445)
                     /locus_tag="ACG9BB_001162"
     CDS             complement(14898..16445)
                     /locus_tag="ACG9BB_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFN2837119.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="ACG9BB_001163"
     CDS             complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="ACG9BB_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFN2837120.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(16890..17084)
                     /locus_tag="ACG9BB_001164"
     CDS             complement(16890..17084)
                     /locus_tag="ACG9BB_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837121.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(17310..18512)
                     /locus_tag="ACG9BB_001165"
     CDS             complement(17310..18512)
                     /locus_tag="ACG9BB_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2837122.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(18515..18709)
                     /locus_tag="ACG9BB_001166"
     CDS             complement(18515..18709)
                     /locus_tag="ACG9BB_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN2837123.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(18756..19604)
                     /locus_tag="ACG9BB_001167"
     CDS             complement(18756..19604)
                     /locus_tag="ACG9BB_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFN2837124.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(19809..20159)
                     /locus_tag="ACG9BB_001168"
     CDS             complement(19809..20159)
                     /locus_tag="ACG9BB_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837125.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            20337..21200
                     /locus_tag="ACG9BB_001169"
     CDS             20337..21200
                     /locus_tag="ACG9BB_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2837126.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            21824..22816
                     /locus_tag="ACG9BB_001170"
     CDS             21824..22816
                     /locus_tag="ACG9BB_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2837127.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            22832..23716
                     /locus_tag="ACG9BB_001171"
     CDS             22832..23716
                     /locus_tag="ACG9BB_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2837128.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            23765..25372
                     /locus_tag="ACG9BB_001172"
     CDS             23765..25372
                     /locus_tag="ACG9BB_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2837129.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            25373..26857
                     /locus_tag="ACG9BB_001173"
     CDS             25373..26857
                     /locus_tag="ACG9BB_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFN2837130.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            27034..28026
                     /locus_tag="ACG9BB_001174"
     CDS             27034..28026
                     /locus_tag="ACG9BB_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2837131.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            28078..28947
                     /locus_tag="ACG9BB_001175"
     CDS             28078..28947
                     /locus_tag="ACG9BB_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN2837132.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(29367..>30899)
                     /gene="guaA"
                     /locus_tag="ACG9BB_001176"
     CDS             complement(29367..>30899)
                     /gene="guaA"
                     /locus_tag="ACG9BB_001176"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFN2837133.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         9690 a         5145 c         6234 g         9830 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
    14821 agtaagcgcc ccatagaaca gtacctgtac gtcaaataac ccgacctata gaatagaatc
    14881 gggaatgttt ggcgttttta gttacgagaa aaatgttgtt gtacatcctt gttccaaggc
    14941 aacagttgat ctaaagtttc tgctgaaagt acgggaagat ttggtatctt atcaaataga
    15001 aactctagat actttacagg attcaatccg ttaagttctg ctgttttata tacactcaga
    15061 atgattcctg aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt tttccgtcct
    15121 atcaccagct ctttgatatt tcgctccgca cgattatttg tgagttctaa acgaccatct
    15181 tcaaaaatag gttcaaaacc acttcgtagt ttacacgcat attgaacggc tgcatccaac
    15241 ttggattttg caacaggatc tatcgttgcc atccactcat aaaaagcaat aaataatgga
    15301 cgcagttctt gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt ccatttcttt
    15361 tctaggacaa accatttatc caattgtttg actgcttgag ctgcgggaat atccgtgttt
    15421 ttctttcctt ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa acaatagaca
    15481 agtcgtacac tttccacggc attatacccg ctatagccgt cacagtgtaa ataccctgta
    15541 aactccttga gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg gtgataataa
    15601 acgatgggat gctgactgtc ttttcctgta caaaactgcc agtaataggt atctgttttg
    15661 gcactttcaa tcacacgata acttgtttca tccccgtgta gaatctcttc ttgattaaga
    15721 tagactctta atcgttctcc taaagggtct aacgcgtttt gcactgcttt aatatgccaa
    15781 ttggtgatat tatctctcga aatatccaac ccatgtgttt gtttccaata gttctcttgc
    15841 cgataggcag gaactttctg accaaatttc atccggatcg tttccgccac taaactattg
    15901 gaacctaagc tattagaaat caaaggttta ggaacgatcg cttttttaaa agaagaaagt
    15961 ccttcttttt cgcagctttt acaacaatag gtatgttgat aatgggtgag acaatccaac
    16021 atagcctgat ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc tttcatctgt
    16081 gaaccgcaat gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt ctctgttata
    16141 ggtaagtgtt tgattttgtc tgctttcgtt cctacaactc gttgatgtcc ttttactgga
    16201 ataatggtgg cagctgcttc tggaacactt acagaaattt cttcatcact aaaaagattc
    16261 aattgcccat tcgtttcctc aaagagagtc tctttagagc gtccaaataa tttgttggtt
    16321 aaataattga ccttttcatt caatagttgg atttcagttg taagattctg aatggtttgt
    16381 tgttgtgctt gattggtttt atgggcttct tgaagcaact gaagcaacgt atctgtagtt
    16441 tccaaaattt ttcaccacct tgaatcgaac tgattttgtt acagaatacc acaagaaacg
    16501 cacaagaaat aggggttcaa cacatttttt gtgttaaccc cccagcttaa aaaactcctt
    16561 tttttcctgg caaaatagct cgaggttgat cgatacttaa tccttctaac aaccatcgaa
    16621 cctgttggtt cgttaaggtt cgaacttcgt ctttcgtccg aggccatttt aacttgccgt
    16681 tttctatccg cttataacag agaagaaaac catcaccgtc ccaatagaga agtttgaaac
    16741 ggtcttgacg gttcccgcaa aataaaaaaa cggcatcttc gtaaagctcg agttgatact
    16801 cttgttgaat aagactggca agaccatcaa ttccttttcg caaatctgtt tttccgcaca
    16861 caatataaat atgtttggtt tgaagtaaat cataaagcat tggacaacag ttctttcaaa
    16921 atagcttgaa gaatataatt gttaataccg ttatagatca aaacttcaga accattctct
    16981 cttttgatgc gcatcgctaa cttggcagcc gcgggtttac ctttttctgg tgcactcttt
    17041 tcgtttccta attggacagg aacaatctca tttaatttat acatagaaaa cctccagact
    17101 tcttgatagc tcaagtatat cttgaggtgc atttgttagt aaggtactgt tctatggggc
    17161 gcttacgttg aaaaacgttg atttatcggc attccccatc catattatat ttacgtctta
    17221 ttcccactca atatgagtag cctcgtaaag aagctattat atcagtgctt tttcagccta
    17281 ttcctcattt tggtagggaa atggtagggt tagattttta ctgcttgatt tccattaaag
    17341 tttgcaactt tagattttgt tgcacaggta aatttaatgt ttcctgtcat ttgttctgca
    17401 attccttcat cattacggct ccatagatga gcgtagtgct ttaaagtgat ttcagggcta
    17461 gaatggccta accgttgcga cacgaccaga atgtctgcgt taaactcatt gattaggtag
    17521 ctaacatgag aatgacgtaa tccttttcct tgtattaggg gtaccttagc tagtttggca
    17581 taacgctgaa taattcgttg aacagttgaa cgatagagtg ggaggtcagt gtagctaaga
    17641 ataaattgtt ctacaccatg atttttttga ataactttcc acttttttag cacttcaatt
    17701 gtatcatcgt ccagagaaat gatccgcaag ccgctttcag tttttgtgta aggtttccga
    17761 ataaaatctt tttgatttgt catttccaag gtgtgatgaa ccctcaactt tttacgtttg
    17821 aagtccacgt cattccaatt aagtgctaat ccttcgctta cacgaacacc agtcatgtaa
    17881 tatagccaga tcattaaaaa acacatatgt tcgtagaaat catctttata aatttttgcc
    17941 aaaacttctt caaattcttc ttttgtccag taaggaacga ttgattttcc ttttggaatt
    18001 gcttttgttc ttttggaaat attctcattc agaaattgca gggtgaccgc atagtctaat
    18061 gatttacgaa acataccgta cataagtgaa ctgtatgctt gtgagtatcc gctatcgttc
    18121 aataaccaaa ttcgataatt ttcacaatct cgcacattga tttcacgaag cttcttatca
    18181 ccgaaaaatt cagtaatctg aatcaatcca cactcacgag aactccaagt acttttttgt
    18241 acgctagctt tgtagtgtgg aacgtaagac tgctccataa attctttgta agttaaatca
    18301 taattcgcaa atccatttac aaccatgtat tcatttttta tacgcatgac ttctttgtgt
    18361 gcttcacgag ctgatgagaa tcgttttcct agagaatctt ttgttccttt cttctggatc
    18421 ctctttccag tcacttgatc tgttcctaac tcaactagat aagaaaagcg accattttta
    18481 tcttgataca cacaagggta ttttgttttc atagttattc ctcctgtgaa agaggaacac
    18541 ctataatttc ttcaattact tgtcggggta ctaccccaac tcttttatta ttataatatg
    18601 gatatccctg atttaccatt cttgtttttg cttgtcggat tattgtggta gcttgatgtg
    18661 ttttaaatcc tagagcctct aaatcttttc gacaaattgt atttttcatt gtataaactc
    18721 actttcaaag aaaacactat cattgggtaa tagtgttatt tctttatttt tgtataatga
    18781 acggtacttt cttgaaaatc catcacttgt tcaggacttc ccaactcatt taaagctaaa
    18841 gcattgtaaa aaaatggacg tctaactata tattcgcttg tatatagtat atcttgattc
    18901 gtggaatcat agggtttctt taccatcttt ttggtgacag ttggtagttt gagattccgg
    18961 gacttgaaaa aagctttttt cttatgctct ttcaattcca gctctttatc gaaatatttg
    19021 ctgatatagc gcccgcgatt ttcgatactg tctacatcga tcttgttgat cttaacgaaa
    19081 ccctgacccc aaatcgtagt taaccggtta aatgagataa aggggaaatc aaacaagata
    19141 atatggtagt ggattgcacc gcgttgctgc ttttcccacg tggcgagata ctttaaattt
    19201 gcttttttag ttttatagag gtcataattc agtcgtttga taaatttttt aaattcatta
    19261 tttgcatact caatgtcttg aatgttttct ttgaaagtga gagttaaaaa tttcgtttga
    19321 ttgtcaaaat tcgtgtctac aaggcgggca atctcaaatc gcatactttg ataatgtttc
    19381 tgtttgcgtt ttaaactgtc atattgtcct tgagcggata attcatcata gttttttctt
    19441 ttttcaagag taatatcatt ttcataaatc caagctaatt tttgttttcg ttccagattt
    19501 acttcgtttt cgatcgtttt acgtttggga gtaatgattg cttcataatc ataaatttca
    19561 atatacgtgg gagtttcaat gattttctga ttataagctt gcaaaaatca tcactccttg
    19621 ttgcgtattc gattgtcaaa gaaccagtca ttttctcctg atgttgatct ataaatcaag
    19681 ttaaggggga aggcctgcgt agcagccctt ctagcctatg gggtgttggg cggtcttaac
    19741 ggacagaaac ggctctgtgg gcgctgcccc gccgtttcgt cctaaccacc caacactcca
    19801 tagtatagtt agttttgtgt agtggtacct tgttttctgg gaatcaaagt tgccgtatca
    19861 gcggttgccg ataacccata actgacaaat tgtccattcg agcgtcccca aggtacaatc
    19921 accaaattgg taaatgtaat tatggctaaa ccttgttggg taagttcttc cggcgtgatc
    19981 acaggatgtt cagatttgac tttgacactg attttttcgt agcgaagttt tggtaggata
    20041 cattcgtagc tccaacctag aatttcgccc tccttttgcc agcgagtgac attgacgact
    20101 tggtattctt tttcgagtgt ttcttttggt aaactgagat cggtaatttt tattggcatc
    20161 acaattctcc ttttttatac gaaagtttat acgatttgat taaaaaatat agatggccat
    20221 ctacgacttg tattatatac gagaacttat acgtagtcaa gagattttct aaaaaaatat
    20281 atacgatttt ttatacgagt gttataatga aaaaaatgat aaggaggaat ttgaatatgt
    20341 ttggtttagt acaacctaat aaagtgaagg tgggccaacg aatcaaagaa ataaaagacg
    20401 gaatgtctct ctcttttaca gaattaggga atcgattagg tttaaaaaag ccgacgatca
    20461 gttcctacgt tcaaggctat gcgttggctc ctaagagtgt gatcaaccaa ttatctagta
    20521 tcagcggaaa acctgtcggc tggttttatt ttgggcatgt ggaagaatat atccgagagt
    20581 atttgtttct cattggtcag aaaaaaatag tggacgatta tcctgaaatc attgagaaaa
    20641 taaagcaaga attttatacg ggcgagttca aaaatcctgc ttgggagaat gagctaggct
    20701 atcctttgga ggaatttatc caagaatgtt ttgtagaata ttcgagtgaa atcatgaagg
    20761 actatgtcgt acagattacg aaagaggtac ttgaagaatc caaaacgcta aacaaactat
    20821 catcaaaagc aaaagaagaa gctgtcacga ttctttcatc tgggatcatt gactatatcg
    20881 agatgagtgg tgagatcaag tatggagaga aagatcgcat tgcgaaaatg gtgaaagaat
    20941 caataaaaac ctttgatgtg aaacaagacc tgacatttaa tgattcctat ttgattggaa
    21001 agttgatcaa tattcttggt acagatgaag atacggagct gtttctttct tctttatcaa
    21061 tgatatttac tgataagcat ttttccactc gatttggtgg ggaagaatta gtcgaaatat
    21121 ttcaatcgat gcgaccagcg ttaatcaaac tctatcggga gaaaacacca gatgaatttt
    21181 atgattggtt tgagaagtaa aataagaaaa gtggcttaga ttacaagttg gtgaaaaaat
    21241 ttttttcagc ataacatcag taatctataa aggggttgtt gattatactg tagaagttaa
    21301 gtactcaaga gattttttgt acgtaaacaa agcaaaaaag ggtgcagttt cactagaaaa
    21361 tgtactcttt tttgtgattt ttcttagtta tagagttttt ttgaaacttt aaaaatgaga
    21421 aaacatattg attttaaaga gtgtagacat agcttctttt gctaaataga ttaaacttac
    21481 tggtgagtgg tcttgaacat acttttaaat cacattttac tgagttttat tggattttat
    21541 aattcattgg aaacctcgta cttaatctgt ttttatctat cgactagaat ctgtatatat
    21601 tccagctatc aatgaggccg cagaaaaggg tgcttaaaaa taatctgttg tttaaaatat
    21661 tattatcaga atacttagat acaaaattct ggcacaaagt aaaaaaaata ttgacttaaa
    21721 aaaatggata tggtaatcta taaacgaaaa tagtggtaac gtttccaaaa tgcatcttcg
    21781 ttaaattttt atttttttaa acgaaaatgg aaacgtttct aaaatgtatt gggaggagat
    21841 tgaattggaa aagacattgg gaccaggtgg tgagataagg gcggtacaac gggataaaaa
    21901 aaatatttcg aagtgggaaa aattaaagaa acagttgcgg cgtgataagt ggatgtactt
    21961 gttgctctta ccaggtatca tcatgacgtt tatctttaag tatgtgccaa tgtacggtgc
    22021 tgtgattgca tttaaagact ataatcctat gctaggaatt ttggaaagtc cttgggtagg
    22081 actagatcac tttagggaat ttatcagttc accaaatttt acgcttttgc tgagcaacac
    22141 attaaaaatt agtatttatg gtttattgtt aggttttttt ccaccaatta ttttagcact
    22201 gagcttgaat caaattatca ataaaaaaat aaaacagaaa atccagttga ttctatatgc
    22261 gccgaatttt atctcagtag ttattattgt ggggatgctg tttttgttcc tatccgcaaa
    22321 tggacctatt aatgggttag tagaaaaggt tacggggcaa ccaattatgt tcatgtctaa
    22381 tccagcttat ttcagaacga tttatatttt atcgggaatt tggcaaggaa tcggctggtc
    22441 atccattctt tacaccgcaa cgctggcggg agtcagtacc gatttatatg aggcagcaaa
    22501 tatcgatggg gcaaatattt tacaaaagat ttggcatatc gatttgcctg cattgaggcc
    22561 tgtgatggtg atcagcttca tcttggccgc cggcggtatt atgaatgtag gatatgaaaa
    22621 agcctacttg atgcaaactt caatgaacat ccctgcctca gaaatcattt ccacttatgt
    22681 atacaaagtc ggcttgcaat caggtgatta tgcctactct gctgctgttg gtctgttcaa
    22741 tatgattatt aatgtcgttt tattactgat tgtcaacaat ataaccaagc ggctaaacga
    22801 tggtcaaggc ttgtaatgaa aggagaaaat tatgaatagt gctttatcaa aaatcgaccg
    22861 tcaaatctta tggttgaatc gagtgattgt aactttttta gtcgtcatca ccattgttcc
    22921 gctgatttat gtactggtag cttcttttat ggacccagct actttgatca ataaaggtat
    22981 aagcttcaat ccgaaagatt ggacaataca gggatatcaa cgggtatttg cagatgattc
    23041 cattttacgc ggttttgtaa attcgttgtt ttactctttt agcttcagct tattgactgt
    23101 atttattacg atgatcactg cgtatccttt gtctcgacct aacttggcgg ggaaaaagat
    23161 tattatgact ttttttgtca ttacaatgtt cgtgggtggt ggcttgatac ctacctattt
    23221 attggtaaag aacttgggca tgttagatag tgtatgggcg attatcgtac caggtgctat
    23281 aaatgtgtgg aatattatct tagcccgtac ctattttagt gctttgccaa aagaactttc
    23341 tgaagctgct actatcgatg gcgccaatga cttgcaaata ctgttcaaag tcatgcttcc
    23401 attagcgaaa ccaattatgt tcgtattgtt tttatatgcc tttgttgggc aatggaactc
    23461 atactttgat gccatgattt acatcaaaga tcccgactta cagccgttgc aactcgtatt
    23521 aagaaatatt ttgatccaaa accaaccttc acaagacatg gtgggagcca atactgccat
    23581 ggcagaaatg aaacaaattg ctgagttgat caaatacgca acgatcgtca tttctagtct
    23641 gccattaatc ataatgtatc ctttcttcca aaaatatttt gataaaggtg ttttagttgg
    23701 ttctgttaaa gggtaagtat tgttcactat ttgtatgaat aaaaattata aaggagattt
    23761 taagatgaaa gcaaaatgga tgctgccaat cgtttttact tccgtactaa ttttgggggc
    23821 ttgtggaggc tcaaaagaaa agagtaaatc aagccctgat tatgaactag aaaatgtctc
    23881 ttttccattg aaagaaaaag tatctttaaa aatgatgtct caaagtgccc ctttggctcc
    23941 caatgatcct aatgacaaat tgattttcca acgattagaa gaagagacca atctccatat
    24001 tgattggaca aactacagta gtgattttgc cgaaaaaaga aatttggata tcgctagtgg
    24061 tgatttacca gatgccatct tcaacgccgc tgcaggtgac tatgatttat taaactgggc
    24121 ggaaagtggc gttattgtac cgattgaaga tttgatcgaa gattatatgc caaatctgaa
    24181 gaaaattttt gatgaaaacc ccgaataccg acaaatgtct actgcacctg atggtcacat
    24241 ttactctatg ccttggattg aagaattagg cgaaggaaaa gagtcgattc atacagtaaa
    24301 tgggatggct tggatcaaca aggagtggtt ggataatttg ggtcttgaaa tgccgcaaac
    24361 cactgatcag ttgatgactg tgttggaggc tttcaagacg caagatccaa atggtaatgg
    24421 tgaagcagat gaaattcctt ttagctttat taacaatgga ggaaacgaag acttaaaatt
    24481 tttaatgggg gcattcggtc taggtgataa tgatgaccac ttagtcgttg ataacgatgg
    24541 tcaggtacaa tttactgcta acaaagaagg gtataaagaa gccatcaaat atttcaatga
    24601 aatgaataat aaaggtctta ttgataaaga ggctttcgaa caagattgga atacgtatgt
    24661 tgccaaaggg aaagaacata agtatggcgt gtactttact tgggataaag caaatatcac
    24721 gggtgcagat gatagttatg acgtcttgcc agtcttagcg ggaccagatg gaacgaaaca
    24781 tgttactcgt acgaatggca tgggtttctc aagagatcgt ttcgtaatta ctagtgcgaa
    24841 taaaaacttg gaacttacag ctaggtggat agataaaatg tatgaaccgt tacagtctgt
    24901 ccaaaataac tggggaacct acggtgatga agcacaggcg aatatttttg aattaaagga
    24961 tggtatgtta aaacatttgc ctttagaggg gacagcaccg ggggaactaa gacaaaaaac
    25021 cgaagctgga ggaccattag cagttcttga ttcttattac ggcacagtca ctactatgcc
    25081 agatgatgcg aagtggcgtt tagatttgat gcatgacacg tatttgccat atattacaga
    25141 agacaatatc tatccacgtg tctttttaga aaaagaagaa acagatcgat tagcaaaaat
    25201 cgaaacggat atgtgggatt acatctatcg caaaagagca gagtggatca ccggcggtaa
    25261 aatcgatgaa gaatgggaag aataccttgc tgaattggaa cgtgtaggtt tggaagaatg
    25321 gattcaaata aaacaaactg gctatgataa ttttttgaag ggagcacaat aagtgttagt
    25381 agaagaaacg gcgattttgg ataaagcaaa tcgatttatt gaaatgatgc gaggaaaagt
    25441 taaccctcag tatcgacaaa actatcattt ttccgcaccc atcgggtgga taaatgaccc
    25501 caatgggttt atatattaca aagggcatta ccatctcttc tatcaatact atccttacga
    25561 tagtcgatgg gggcccatgc attgggggca tgcgcgcagt agtgatttgg tccattggga
    25621 agatctacca gtagcgttag ccccagatca accttatgac agcggcgggt gtttctcagg
    25681 aagtgcaatt gaaagagacg gaaaactata tttattttat acaggctgtc aagaaattga
    25741 tggtattagc catcagcatc aatgtttggc ggtctctgaa gatggtattc attttagcaa
    25801 atacagtggt aatcctgtgg tagcagaaaa acagctaggt gaaaatgggc taatacaaga
    25861 atttcgtgat cctaaagtaa ttgttcacaa cgatggttac tatatggtag tagctactaa
    25921 atctacacag tctactggta gaatagtgat gtttcaatcg tcagatttgt atacttggca
    25981 cttttactct gtgctgctag aagggacttc agagcaagga ataatgtggg aatgtcctga
    26041 tttattccat ttagacggac gagatgtctt aatcatgtct ccgatacaaa tcaagaagaa
    26101 cggactggaa tatcataata tcagttctac catggcttgt attggtgagg taaactggga
    26161 aacaggtcag atgatagttg ataatttcca tgaaatggat tttggaatgg atttttatgc
    26221 accacaaaca acaagagatc ctaatggtgg acgggtgatg attgcttgga tgcagatgtg
    26281 ggatcgtaca ttgcctacac atgagttagg ccatctttgg gcaggctcta tgagtttgcc
    26341 ccgagagtta agagtgaaaa aaaatcgatt ggtacaaaaa cccatctcaa ctgtatatag
    26401 tcatttagtt tatcaacaag gcagtgaaaa tattgcagtg actgacacat cagtcgtatt
    26461 tcgaaacgcg ctgtctgatt cttcatattt acatctagtc gcagatttgg agaaagctga
    26521 agtttttaca attgaattac tgcggtctaa gacggattgt ctcaaactag cctatgacac
    26581 tgctacacag gtttttgaat tggatcgtca gaaactagga agagagctta caggggcgga
    26641 agaagaccct ctgaatgtac gaaaagttga agtacctttg attgataatc agctcgttct
    26701 tgagattttt agagatacct catcaattga agtctttatt aatgggttag aagttatgac
    26761 ggctactttc tatgagctgg aaaaagctcg agatatcttg tttcattcga tcggagaaag
    26821 tcgtattcaa gcttttgaaa cagcgagtgt gaaataaatg tttgattgct taggcgatga
    26881 atcaattatt taataggttt tgaaggataa tgtttttaga aaagaacaga tagctagatt
    26941 tcccatggat ttgctattag tattttgaaa aaattagtaa tttatgacaa aattcattat
    27001 actaaacttg tgcagaaatg tgagggatca actatgaaag caacaatgaa agatgtcgca
    27061 aaattagcag gtgttggagt aggaactgtt tcccgggtat tgaataacgg ttctgttaaa
    27121 gcttctacta gagaaaaagt tgaagcagca atgacagagt taaactatca accagatttt
    27181 tatgcccggg ggttaaagat gaagcatacg aacacggttg ctttgattct acccacggta
    27241 tggcatccat tttttggcga atttgcctac catgtagaaa cagcattaga aagacaaaac
    27301 tacaagcttt atttgtgtaa ctccagtggt aatagtgaaa aagaacaaga atatatccag
    27361 atggtcagcc aaaataaagt agacggcatc attggaatta cttattcaga tatcgatgaa
    27421 tacatttcga gtaatctgcc ttttgttagt attgaccgtt atttcactga agaggtgatt
    27481 tatgtgaccg ctgacaatgc ggccggtgga cgtttggctg caaatgaact ttatcgccga
    27541 ggatgtcaga atgtagcata tatcggtggg taccaagaaa caccgaatga aacgaagagt
    27601 cgtcgaaaat attttgaaat cgagtgccgt caactgggga tttcctataa agcgttagat
    27661 atgttagaac ctttagacca tctctcagaa cgtgtaacaa attttttgtt agagaatccc
    27721 gatatcgatg ggatttttac gatcaacgat ccaatggcat tagctgtcat tgatatgctt
    27781 gaaaaaatag ggaaaaacgt accagaggat gtccaagtta tcggatttga tggtcaaaag
    27841 atgacagaag gagatcctta tctagtctca acgattgccc aacctacaaa gcaaatggcg
    27901 gaagtggcag tggcaaaatt aattgctgtc attaatggag agacagtaga aaaacgtacc
    27961 gtattgccga ttgaatttgg cgaaggtgac acaacgaaac ctttagtgag aaacgatttt
    28021 gcttagtgca aatacgtttc tttttttgaa aatgagtgaa tgaaaggtgt agataaaatg
    28081 ttatatggtg gaatagaagc cggtggaaca aagtttgtct gtgggattgg taatgaacaa
    28141 ttacaagtga tagaacggaa aagttttcct accacaacac cagaagaaac gatggagcaa
    28201 gtgtttgatt tctttgataa gtacaaagga aatatcgcat caattggtgt aggagcattt
    28261 gggcctattg atattcaaat cgaatcaaga acttacggat atatcaccaa cacaccgaag
    28321 ttagcttggc aaaattatca ttttgtagga gctttggaaa acgccttgtc ggttcccatc
    28381 tattggacta ccgatgtaaa tgctgcttgt tatggagaat atatggcagg gtatggaaaa
    28441 ggtaaaaata gtgttctgta ttacaccgtt ggtacaggtg ttggtggtgg tgctgtagtg
    28501 gatgggaaaa tagtcgcagg ttttagtcat cctgaaatgg gacatatgct cttacaacgt
    28561 cataatgaag atcaatttga aggaatctgt ccttttcatc atgattgttt ggaagggtta
    28621 gcatcaggac ctgcgattga aaaacgcttt ggaagaaaag cccagactat ctcatttgat
    28681 catccttatt ggacgattga ggcagattat cttgctcagt gtgtgtataa cacaacgtta
    28741 ctatatgcac cggaagtaat aattttaggc gggggtgtaa tgaaacagcc gcaactactc
    28801 gagttgattc gccaatcgtt taaagaaaaa atgggttcct acgtggatac gcctactctc
    28861 gaagaatatc tactaactcc aaggcttgct gacaatgcgg gcttgattgg ctgcctagct
    28921 ttagcaataa agaaactcaa tcattagaat agtatttatt agaactattc ataacatacc
    28981 ggaagtttaa aagggtattc tgaaacttaa atagtctcaa ttcattcttc tttcttgaga
    29041 atctgagtag agacttttat tctgtttcaa attaatataa ttacgaaatg atattgagta
    29101 caagaaagtt ctttttacca gatcttttaa atcgaaaaat ctgatatata tatttatagt
    29161 actaaaaatc tgattagata aaaagctaat tcaataggaa aataaaggta gtgaataaaa
    29221 gcaaagccct tgttagtagt taatctaaca agggctttgg tagggaaaat ggtaggaaac
    29281 catcttattt tttacgaaaa tcaggaactt tagtaattga ataatgttga tttataggta
    29341 ttcctcatcc atattatgtt ggctctttat tcccactcca ctgttgcagg tggtttgctg
    29401 gtaatatcat acacgatacg gttaacgtgt gcgacttcat ttacgatacg gacactgatt
    29461 ttttgcaaga cttcccaagg gatacgggca aagtcagcgg tcatgccgtc aatagaagtg
    29521 acagcacgga tacctacagt gtagtcatac gtacggccat ctcccataac cccaacagag
    29581 cgaatgcctg gtaggacggt gaagtattgc cagatgtcgc gatctaaacc ggcattggcg
    29641 atttcttcgc gcaagatagc gtctgattca cggacgattt ctaatttttc ttctgtcaat
    29701 tcaccaagga cacgaatacc taagcctggt ccagggaatg gttggcgcca aacgatgctg
    29761 tcaggcatgc ctaattgggt acctaattca cgtacttcgt ctttgaacaa tgtgttcaat
    29821 ggctcgatca gcttgaattg catgtcttca ggtaatccac ctacattgtg atgtgatttg
    29881 atggtttggg cagtttcagt tcctgattcg atcacatcgg tataaagggt tccttgtgca
    29941 aggaaagaaa cgccttcttc accggcaagt ttggttgctt catcatcgaa gacatataca
    30001 aattcatttc caatgatttt acgtttttct tctggatcag acacgccagc aagttttgat
    30061 aagaaacgtt ctttggcatc gacttttata atgttcaagc caaattttcc gccaagactt
    30121 tccataactt gttctgcttc acctttacgc aacaatacgt gatcaacgaa gatacatgtt
    30181 aattgatcgc cgatcgcttt ttgtaaaaga acaccaacaa cagaggaatc tactccgcct
    30241 gaaaggccta ataaaacttt tttgtcaccg acttgttcac gaatttttgc gatctccatg
    30301 tcgatgaagt tatccattgt ccagtcacca gtacattggc agacatctag tgtaaaatgg
    30361 cgcaacaagt catttccata ttcggaatgg cgaacttctg catggaattg gattccgtac
    30421 attttgcgtt ctgcattttg gatcgatgcg atggggcaat ctttgctggt agcaacgaca
    30481 tcgaaatcag tcggtgcttg ggtaactagg tcaccatgac tcatccaaac ggtttgtttt
    30541 tcaggagtgt tttggaaaag ggccgcttct gcagaagtga tttccaattc tgcttttccg
    30601 tattcacggt ttttggctgg tttaactttt ccgcctaatt tataagtaat caactgcata
    30661 ccgtaacaga ttcctaaaac tgggatcccc aaatcaaaaa tcttttcatc aataccaaag
    30721 gccttctcat cataaacact gtttggtccg ccagaaagga tgattccttt agggttcatt
    30781 gcctttactt cttctgcggt aatgcggtgg ctcatcaact ctgagaaaac accgaactca
    30841 cgaatacggc gagtgatcag ttggttatac tgactaccgt aatcaagtac gataatttt
//
LOCUS       DAZAFF010000028        30090 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000028 DAZAFF010000000
VERSION     DAZAFF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.028"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..1261)
                     /locus_tag="ACG9BB_001177"
     CDS             complement(29..1261)
                     /locus_tag="ACG9BB_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFN2837134.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="ACG9BB_001178"
     CDS             1516..2085
                     /locus_tag="ACG9BB_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2837135.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="ACG9BB_001179"
     CDS             complement(2263..2703)
                     /locus_tag="ACG9BB_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFN2837136.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="ACG9BB_001180"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="ACG9BB_001180"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFN2837137.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="ACG9BB_001181"
     CDS             3657..4580
                     /locus_tag="ACG9BB_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFN2837138.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="ACG9BB_001182"
     CDS             complement(4805..5044)
                     /locus_tag="ACG9BB_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2837139.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="ACG9BB_001183"
     CDS             complement(5049..5468)
                     /locus_tag="ACG9BB_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN2837140.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..6933)
                     /gene="mef(H)"
                     /locus_tag="ACG9BB_001184"
                     /gene_synonym="mefH"
     CDS             complement(5695..6933)
                     /gene="mef(H)"
                     /locus_tag="ACG9BB_001184"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFN2837141.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7040..7675)
                     /locus_tag="ACG9BB_001185"
     CDS             complement(7040..7675)
                     /locus_tag="ACG9BB_001185"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2837142.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7789..7881)
                     /locus_tag="ACG9BB_001186"
                     /pseudo
     CDS             complement(7789..7881)
                     /locus_tag="ACG9BB_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8017..8652)
                     /locus_tag="ACG9BB_001187"
     CDS             complement(8017..8652)
                     /locus_tag="ACG9BB_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2837143.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9164..10306
                     /locus_tag="ACG9BB_001188"
     CDS             9164..10306
                     /locus_tag="ACG9BB_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN2837144.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10299..10505
                     /locus_tag="ACG9BB_001189"
     CDS             10299..10505
                     /locus_tag="ACG9BB_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFN2837145.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10584..11777
                     /locus_tag="ACG9BB_001190"
     CDS             10584..11777
                     /locus_tag="ACG9BB_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2837146.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11982..13121
                     /locus_tag="ACG9BB_001191"
     CDS             11982..13121
                     /locus_tag="ACG9BB_001191"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFN2837147.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13114..13914
                     /locus_tag="ACG9BB_001192"
     CDS             13114..13914
                     /locus_tag="ACG9BB_001192"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN2837148.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13914..14741
                     /locus_tag="ACG9BB_001193"
     CDS             13914..14741
                     /locus_tag="ACG9BB_001193"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN2837149.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14746..15453
                     /locus_tag="ACG9BB_001194"
     CDS             14746..15453
                     /locus_tag="ACG9BB_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN2837150.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            15553..16419
                     /gene="rfbA"
                     /locus_tag="ACG9BB_001195"
     CDS             15553..16419
                     /gene="rfbA"
                     /locus_tag="ACG9BB_001195"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFN2837151.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16433..17005
                     /gene="rfbC"
                     /locus_tag="ACG9BB_001196"
     CDS             16433..17005
                     /gene="rfbC"
                     /locus_tag="ACG9BB_001196"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFN2837152.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17027..18055
                     /gene="rfbB"
                     /locus_tag="ACG9BB_001197"
     CDS             17027..18055
                     /gene="rfbB"
                     /locus_tag="ACG9BB_001197"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFN2837153.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18153..19004
                     /gene="rfbD"
                     /locus_tag="ACG9BB_001198"
     CDS             18153..19004
                     /gene="rfbD"
                     /locus_tag="ACG9BB_001198"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFN2837154.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19038..21071
                     /locus_tag="ACG9BB_001199"
     CDS             19038..21071
                     /locus_tag="ACG9BB_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837155.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21115..22395
                     /locus_tag="ACG9BB_001200"
     CDS             21115..22395
                     /locus_tag="ACG9BB_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFN2837156.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22605..23411
                     /locus_tag="ACG9BB_001201"
     CDS             22605..23411
                     /locus_tag="ACG9BB_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2837157.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23423..24643
                     /locus_tag="ACG9BB_001202"
     CDS             23423..24643
                     /locus_tag="ACG9BB_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2837158.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24633..26219
                     /locus_tag="ACG9BB_001203"
     CDS             24633..26219
                     /locus_tag="ACG9BB_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN2837159.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26258..28396
                     /locus_tag="ACG9BB_001204"
     CDS             26258..28396
                     /locus_tag="ACG9BB_001204"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN2837160.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28764..29747)
                     /locus_tag="ACG9BB_001205"
     CDS             complement(28764..29747)
                     /locus_tag="ACG9BB_001205"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN2837161.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         9614 a         5278 c         5975 g         9223 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat tacacctact tcacgaggtc ttttttggtg
     6361 tggaatatgg ataaaaattt ccataatggc tgaaaaagta aaacatataa tactaacaat
     6421 taaaatggga ttgataccat aaaattgaaa aactactccg cttatagctg gtccgataaa
     6481 gtttgctaaa gtatttactt gatttattat agcattaccg acggtaagtt tatctgatga
     6541 taccaataga ggaatacttg cttgtaccgc tggttgatat gcaccagaaa taccataaag
     6601 taacatcaaa gcaacaataa acaaaggtat gataggaatt tttcccaatg acaaataaaa
     6661 tactaaaatt atgattgctg ttacaaaatc tagtatgacc ataatgtttc ttttatttgt
     6721 cctgtcagca ataataccac ctaccagcga caaaataacc ataggtaaaa aagagcaggc
     6781 tgtagcaata ccaaacaaag ctgatgaacc agtttctttt aacaaatagt acggtaatgc
     6841 aaatcgtagg atagcgtttc caaataaaga aattatctgt cctataacaa caagtataaa
     6901 atcacgatta aaaagttttt tttctgcttg cataaaatct ctccttttta tttaatcaaa
     6961 cagaccgtcg gtttatataa tggtttaaat aaactgctct tttatagagc agtctatcgt
     7021 atagggataa caaatacagt tatttacaaa actgtataaa tgcagaaatt gtttcttcgt
     7081 ctaataatgt gtctatgcct attccattta aaagggcatt gaatgtattg caacgttttt
     7141 ccataccctc tttatccgtc atttcaacta atgggttgag ccatacatta cttaatacca
     7201 tgataacttc tgcaatttca tgtgaatttt caatctccaa agacttatcc ttgatacctt
     7261 gttctaaaac gggttcaaca aattcaggta ctacaatttc atacatttgt tctatttgca
     7321 tagcaagaaa acgaggattg tttaatagtt gaggagaaac tgtcaataaa acgctttggt
     7381 tagaattgaa taccgctgtc ttaaatattt ctcttaactt ttctaaaccg tttaaactat
     7441 cgttatctct aattttagca aatattactg cattttcatt tccaattctt tgaaaaattg
     7501 cttttaaaat atcttccttt gaatcgaaat gatgataaat agcaccttta gaaagttttg
     7561 tttcattgat aatatcttgc aatgatgtgt tatcatatcc tttttcacaa aacatttttg
     7621 tagaaacatc taaaatgagt tttactgttt cttctggata tttatttcgt gccataactt
     7681 acctccaata caaaccgttg gtctgattaa agtatatctt agtattttat gcttgtcaat
     7741 ataaagactt gcaattttat aaaaatgcag aatgcaagcc tttttcattt attctacaat
     7801 cttccagttg ctttccttat gcggtgtcaa ttcatactgt gatacctgcg ttgcctttgc
     7861 ctgatttacc acaaatcaga ttttatccct ttcttcctct tgattgccca caacagaaaa
     7921 gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc tttgcactct tgatcgggct
     7981 ttttgtttgc tgtatcttct cgcaagagga aaaaagttac tcgtcctcat catcaaaatt
     8041 gttattcctt aatacttctc gtaacagttc catatcttct tttgagttcg ggtttatcat
     8101 tttcacgact tgataaggtg tcttatactt atgtccctct atgctttccc caaacatatc
     8161 catactgtat aaatcatcat agcggtctaa taatttttga aacttcaaat gctggataaa
     8221 ttctttgatt gggtaaaggt cggacgcttc aatgcttttc ccgttgatat agtctgtaat
     8281 atattcccgt aacttttcta actcatattc cagccattct tcctcgctgt caaagaagtt
     8341 atcatagtgt ccatgtttca gttctgcatt tagacgttct tcggctctta aaaactgaca
     8401 gacttccttt tcagattgat tgacatactt ccattctccc gacaacaatt cattgggcgt
     8461 aacgtccagc acttccatta tcttttccag cgtttcatag gcaggatagt tcaaccctct
     8521 ttcaatcttg gaaaggctct gcatattgat accgattttg tccgcaagtt cctgttgttt
     8581 cattccccta aattttctta tggtctgtat attatttcct aaatggctta ttttcattgc
     8641 ataaccctcc atatctcgtt atatcttcgc tattataagt ctatcatgct taatatgatt
     8701 tgtaaaggca aaaaaagata ttgacaaata agcatataag aattactatg ttcttgtaat
     8761 cgaataagca caatagaatg aaatataaaa acattcaaaa agaaatacct atctgattaa
     8821 atccgtagat atgaatatcg gttgaaatgg aacttgttag aaaaggcaaa acagaacttg
     8881 ctgacaaagt atttctgaca cggtggcgga agcctctcag gcttaccgcc atgacacctt
     8941 gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg cgagccttgt ctgtgccccc
     9001 gttatctaac aggggggcac tatttcacaa aagacaagtc aaaaaccata cacccccaaa
     9061 cctgtgagta gtaatgagag gttttaagaa gatagtacat atcacacttt ttgaaaggtg
     9121 gtttacaggt atttcaaggc tattgtggga ttggaggaat acaatgaaca atttagaatt
     9181 tgcacttgaa atgaagaaca aacgtcttgc cagcggtctt tcacaaggag aacttgcaag
     9241 tcttacccat gtatcaagat atagtatcaa ccgctttgag aacggaaaag ctaacgccag
     9301 caaggaaaca cagaacatta tcctgcgttg tctgaattac tatatctgcg ataagccttt
     9361 ttatctgctc gttgattatc tgtctgtccg ttttccaacg actgacgcat tggaagttat
     9421 ccgaaaggtg cttggcatga aagcagatta ttttatccat tatgactatg ggtattatgg
     9481 ctataaggag cattatgctt acggggaaat caaggtcatg gcttctgatg atgaacacat
     9541 gggcgtattt ttagaattaa aaggggcagg ctcacgcaac atggaatatg tcttacaggc
     9601 acagaacagg gactggtatt cattcttaaa ccgctgtctt gactgtggcg gtgttatccg
     9661 acgttttgac ttggcaatca atgatatgtg cggtctgctg gatattcctg tgctatctga
     9721 aaaatacaga aatggtggtg cggaatgtcg ctgtaaaaac tatgaaaatg tacaaggtgg
     9781 aaaattaagc ggaaaaaacc gtaatttagc aagtaccctt tatatcggct caaaggcaag
     9841 cacaaaatat ttctgcctgt atgaaaaaca aaaggaacag gcaacgaaaa agaaacatac
     9901 cgatattatc aaccgttttg aaattcgtct gcgtgacaaa aaggcggtac aggcagttga
     9961 agaactgtta ttgacgtata accctcatgg gctggtgttt tatctcatta ccgattttgt
    10021 ggaatttcct gactatccat tgtgggagat attcatttcc catgacagtt taccttttga
    10081 aatgaaccct gtacctgtca atatggaacg cactctgcaa tggctggaaa gacaggtcat
    10141 gccgtcaatc gtgatgatag aggaaatcga caggctgaca ggttcaaact acatgaaaat
    10201 gattgatgaa tgtacccacc tttctgaaaa acaggaaatg cttgtggaac agatgtgtac
    10261 ggacatatca gacgtaatcg aaagtgaggg ggtgttttat gagtaataca caggatattc
    10321 ccgtatggga aaaatacacc cttaccattg aagaagcgtc aaagtatttc cgcattggag
    10381 aaaacaagtt aagacgattg gcagaggaaa acaaggacgc tggctggctc attatgaatg
    10441 gcaaccgcat acagattaaa cgccgacagt ttgaaaaggt tattgataaa ttggacgcaa
    10501 tctaatgcaa atgagccttg tatgtgttat gatgaacaca agtcatatca aggctctttc
    10561 caacaaggaa aggagcagac accatgaaag aaaaaagacg ggataacaaa ggtcgtatcc
    10621 tgcatactgg agagagccaa cgaacagacg gaaaatactt atataaatat gtagacgcat
    10681 tgggaaacac aaaatatgtg tatgcttgga gattgacacc cacagacccg acaccaaagg
    10741 gaaaacggga aaaaccctca cttcgagaac tggaacagca gataagacgg gatattgagg
    10801 acggtatcga cagcacaggc aagaaaatga cgctttgcca actctatgcc aaacagaacg
    10861 cacagaggac aaatgtgaag aaaagcacac agaaacaacg ggaacaactc atgcggttat
    10921 taaaagagga caagttaggt gctaggagca ttgatacgat aaaaccctct gacgctaaag
    10981 aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa taccattaac aaccataaac
    11041 gctcgttaaa agcgtcattc tacatagcca tacaagatga ttatgtgagg aaaaaccctt
    11101 ttgatttcaa gctaagtgaa gtcatagaaa atgataccaa agagaaagtc gcattgacag
    11161 aggaacagga gcaagcctta ttgtcattta ttaagacgga caacgtgtat cacaagcatt
    11221 atgatgatgt gctgatactg ttaaagacag gacttcgtat ctcggaactg tgcggactga
    11281 cagtagccga cattgatttc aagaatgaag ttgtgattat cgaccaccag ttactaaaga
    11341 gtaaggaaca gggctattat attgaaacgc ctaagacgaa aagcgggata aggcaagtgc
    11401 cattaagcaa agaaacgata caggcatttc aacgggttat caacagccga ccaaagataa
    11461 agtcgataga ggtagacgga cacagcggtt ttgtgtttat caatccgaaa ggtaatccaa
    11521 aagttgccat tgattataac gccttattta tccgtatggt aaagaaatac aacaagcacc
    11581 acaaggacaa tcccttgcca catattacac cgcatacgct acgccataca ttctgcacaa
    11641 ggctggcaag caagaacatg aacccgaaag atttacagta tatcatgggg cattcaaaca
    11701 tcagtatcac aatgaactgg tacgctcatg cgtccataga taccgcaaaa tcagaggttc
    11761 agcgtctaat cgcatagaag tatttaccac gattttaacc acgattgaca gggaaaatat
    11821 aagaagatag acctagatat gcgaggttta ccacaaaagc aaaatgcccg tagagccgat
    11881 aaaataaggc tttgcggaca tttgagaaga tataaaaaga tagtcaaaaa gacatatata
    11941 atttataata attatgactc ttaattaaag taggtgccca tatgcttgtt tcttttagta
    12001 tggttataag attttttctg acaattttat tatcactgat attaacacct gtattcaaaa
    12061 tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata aataaagttc
    12121 caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg actttattct
    12181 tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct tctttagtaa
    12241 tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa aaaagtatag
    12301 ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt gattccttca
    12361 cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta acgatttttt
    12421 ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg ctggcggcag
    12481 ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc ctccatgctt
    12541 ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc ggtttttttc
    12601 cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta ttcctagggt
    12661 ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt tcatcgattt
    12721 ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc attaggagaa
    12781 aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac cgactaatca
    12841 gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct ttgatgtttt
    12901 ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcata ttgattattg
    12961 ccatgttgtt tgctgcaatt ttattatttg aattgattgg tctgatcaat gagaaacatc
    13021 agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg caagtaaaac
    13081 agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa ccagccgatc
    13141 cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga taattttatt
    13201 tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa ttcacttccc
    13261 gagtacaaaa aaagactaac agagtatagg aaatatccct acatcgatat ctattttcat
    13321 gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag cgattctcgc
    13381 tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac agcattggtc
    13441 ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct taatgaagat
    13501 ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt tttgcgcttt
    13561 gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt tgaatgccga
    13621 ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt tatggtagct
    13681 gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat gtactttgaa
    13741 gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta cacacccttt
    13801 tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact gtttcgcgtg
    13861 tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt ctgatgaaac
    13921 cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct ccagcttatg
    13981 gaccattaag caaagcactt agcgaaaagc aaattcagct tattatttac gataatagtc
    14041 ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat cgtttatcat catgatccgc
    14101 aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcagat gaaggaacac
    14161 gttgtctcgt cactctggat caggatacga ggctagtcga cgattatttt gaactattgc
    14221 gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt tctgggagcc
    14281 gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa gcagtagaag
    14341 ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc tggtctgtca
    14401 aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt ttggatcatt
    14461 ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct gtacgaatgg
    14521 aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac cgttctattt
    14581 tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc agtcaccgac
    14641 gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg catatctggc
    14701 ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg ttcggtttgt
    14761 atagctacct ttaatggagg cagatattta agagagcagt tagatagtat tttatctcag
    14821 ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga tacatgggat
    14881 atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg gcctcgtcaa
    14941 ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct aatctttttg
    15001 gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga ctattttgag
    15061 cagaatccgg atcagctagt agtagtttct gatttaatca ttgtggacag cgagctgcaa
    15121 gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct taataatgtg
    15181 ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa acccgatatt
    15241 ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct gggaggaaag
    15301 aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt aaacgcgagt
    15361 gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtgttca tctgattacg
    15421 gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc cttaatattt
    15481 ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa tcaaaaagga
    15541 gagcagatat ccatgaaggg aatcattctt gctgggggca gcggaacacg tctgtaccct
    15601 ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat gatttattat
    15661 ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc tgattatttc aacgccacaa
    15721 gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact gcagatcgaa
    15781 tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg agaagaattt
    15841 attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg tggcggtctg
    15901 tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg cgacagtatt tggctatcat
    15961 gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca agctttgtcg
    16021 attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct ttatttttat
    16081 gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg agaactagag
    16141 attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga acttatggga
    16201 cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc atctaccttt
    16261 atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga aattgcttac
    16321 cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt gaaaaaaaat
    16381 caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac agatgaaagt
    16441 aacagataca aaattaacag atgtcaaaat cgtagaaatg gacgtctttg gcgatcatcg
    16501 cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat tgaaatatga
    16561 ctttgttcaa gacaaccatt cactatcagc tgaagctggt gtccttcggg ggcttcattt
    16621 ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag cagttttaga
    16681 tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg gatttatctt
    16741 atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg gattcgttac
    16801 tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg cagaagcaga
    16861 tgggggcatc gcatttgatg atccagattt agcgattgat tggccaattg atccatcaag
    16921 agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg aagcagaaaa
    16981 tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga aaaacatcat
    17041 tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg taaacaatca
    17101 tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca aagagaactt
    17161 ggctggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag atgcagaatt
    17221 agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag aatcccataa
    17281 cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg gtacgtatac
    17341 acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta ctgacgaggt
    17401 ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg aaggagaaaa
    17461 attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa aagctggttc
    17521 agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca tttcaaactg
    17581 ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc aagtaacgaa
    17641 tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc gtgactggat
    17701 ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca gaattggcga
    17761 aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg aattgatctt
    17821 agaaatgatg ggtcaaccaa aagatgcgta cgaccatgta aacgaccgtg caggacacga
    17881 cttgcgttat gcaatcgatt cgacaaaatt acgggaagag ttaggatgga agcctgagtt
    17941 tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac atgaagactg
    18001 gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac aataatacag
    18061 agaaaatgtc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc ctgtctcttt
    18121 tttaaaataa aaatcaagga gcggataatc atatgttttt attaactgga ggaaatgggc
    18181 aactaggtac agaattgcgc catttattag atgaaaaaaa tctgcaatat gtttcaacag
    18241 atgcaaaaga cttggatatc acagatgccg agaaaacaat ggcttacatc acagaattaa
    18301 aacctgatgt tattttccat tgtgcagctt acacagcagt agataaagca gaagaagaag
    18361 caaaagaatt agatgagaag atcaatgttg atggtacaag aaatgtagca cttgcagcaa
    18421 aagctgtgaa tgctcaattc gtctatatca gtacagacta tgttttcgat ggaaaaaaca
    18481 aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat ggtcgtacga
    18541 aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc atacgaactt
    18601 cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc ctagcggaaa
    18661 cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg acacgtactt
    18721 tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt tatcatttgt
    18781 cgaacgaaaa tagctgttct tggtatgaat tcgcaaaaga aatattgaaa gatacagatg
    18841 tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt ccgcagtatt
    18901 cagtcatgag cctgaaaaag acagaagagt taggatttgt cataccaact tggcaagaag
    18961 cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct tttatcaaat
    19021 agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa
    19081 aagcgaacca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc cgctatctta
    19141 ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt tcacttggtg
    19201 tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg atttttggac
    19261 agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac ctctcacaag
    19321 ctgaagagga ctttccgttg gtgaacgagg attattcatt aaacggacaa aacatgatca
    19381 ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc aactggggat
    19441 tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca
    19501 tggtcttgct tggattcgaa gtactgatga tattaacgaa gagaaaaaaa gctctatcgc
    19561 taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc atgcaacatg
    19621 tgggcgattt gattttcttt actttgggac tgatgactgc tttttatcat tatttctaca
    19681 atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt ttcggtattg
    19741 ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg ctgatttttt
    19801 attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg ttggatgtag
    19861 tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc tggctaacgt
    19921 caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg gtaagtactg
    19981 gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa attccttttg
    20041 aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt tttccaactg
    20101 tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg
    20161 gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt gggttgccaa
    20221 aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc atccttactt
    20281 ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg agagaagatg
    20341 ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct tatttctatg
    20401 cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc gcagttgctg
    20461 tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg ttttacctcg
    20521 cttttgccgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta gaaggtacag
    20581 gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg
    20641 gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg ggagtgaaat
    20701 ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc gatccgaaag
    20761 aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc gctaaggaag
    20821 atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat tttgaagacg
    20881 cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat tacaaccaat
    20941 tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat atgattttta
    21001 aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa aatcagctag
    21061 tcgtacagta ataggctagc tgagaataat cctgaaatgt tagtagaaag gagagtggag
    21121 ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg
    21181 ttactgacgc tagtcttttt tgtttggaca gcagggttag gttttccagc aactttgttt
    21241 atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct cattttaaat
    21301 gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc ccttttcatt
    21361 ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt actgtttcct
    21421 ttgattattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt
    21481 atttatctga ttgtcttttc ggtcttcctc ggaatcacgt tatatcgtgt atttacagag
    21541 atccaagtac cagaaggaca aagcatctgg gctccaggca attcgctttc tgatatttgg
    21601 atcaatacga atataatcgg aagttctttg atgactcttg ctttattgat cagtggattt
    21661 gccagttctt ttgaacgatg gtatgtacga atcatcggtg cagtagcagt tgttgcagct
    21721 tttctcggga tttgggtctg ccaatctaga ggtgcattag tcgcgttgat tgtttttgcg
    21781 atattggatc ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt tattgcttac
    21841 acggttacga ttcttttagc tttgccgatt tcttatctgg cggctgtctc tgaaaaagtc
    21901 aatttgttta ccggtcgaga agatatttgg cataaattct atcagacgct aggagaaaaa
    21961 tctgaacaaa ttttactagg aatgaaaaca tttatcttcc aacgaggaaa ccaattttta
    22021 ggaaaccaca atagttataa ttctattttg aatatctatg gattgatagg atttgggatt
    22081 gctgctttgc tgcttatcct ttttattggt cgcttaacgt tgaaagcaga tttatccaac
    22141 gggcagatga cttttatctg ggcctttttt gcggtgatga tgcagtcatt tatggaggat
    22201 acattgactt catacccatg ggttccgatt atttatatcc tgttggctat ggccgttcat
    22261 cgatatgatg aaccagaagt tgcggaggaa ttctatgtta tggaagaggc atatgacgat
    22321 atgacttatg atgatacatc ttatgaggaa caaccagata gcgctcattc ttccaggatc
    22381 caacgctatc attgataggc acaaaatacg agagaggctg tgacaaccgt cgcagccttt
    22441 tttggtttat tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg
    22501 tatcatagaa aattaagaaa gaaataagaa cgcagctcgg taccaaaaat atggtaaaat
    22561 ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt
    22621 atttatttat gaaggacatt tatcagaaca gaaaattatt gctacagttt tcgatcaatg
    22681 acttcaaatc aagatatgca ggatcgtttt taggaatctt atgggcgttt atcaatccgc
    22741 tatttactgt attgatttat tggctggtat ttggtgttgg actgaaagca caaatgatcg
    22801 atgcaaaata cccatttatc gtatatttag ttaccgggat gattccctgg ttcttcttct
    22861 cagatgcatt tgcatcaact acggttgttt ttcgggaata tacttattta gtaaagaaag
    22921 tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct atccaatctg tacacccact
    22981 tattcttcat tctgattggc atagctgtcg cgcttataaa tggtatatat ccttcagcaa
    23041 tgtctttaca gctgatttat tatttatttt gcatgtgtgc tttcttgaca ggattgactt
    23101 ggctgacggc atcgatccaa ccatttttac ctgatatcat gcaattaatt acgatcgtgc
    23161 tgcagttgat catgtggaca ttaccgatat tatggagccc taatcagttt gattcaggtc
    23221 ttgtaagcat attgaaatta aatccgctgt actatgttgt tcaaggttat cgtgagtcat
    23281 ttttaagtga agcttggttc tgggagcatg gaacatatac tttgtatttc tgggttttca
    23341 cgttagtaat gttcgtaatc ggctctgcag tgttccgtag attgaaacca catttttcag
    23401 atgtgctgta aggagaatag ctatgacaga atatgcaatt agactaaaag atatcacaaa
    23461 atcttataat atgtacgcaa aaccttcgga tcgatttcgt gaagccctca atccttttaa
    23521 gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg
    23581 tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat
    23641 tacgggagta ctgacaccaa catccggcac gatggaaatc gaaggaaaaa tctcagcttt
    23701 gctagaacta ggatcaggat tcaatcctga gtattctggc tatgaaaata tctatttaaa
    23761 tggaatggtt ctaggatttt caagagaaga agtcgatgaa atggtcgatg atatcattga
    23821 atttgctgac atcggagacc atatttatca acctgtaaaa acttattcaa gcgggatgtt
    23881 cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc gatattttga ttgtggatga
    23941 agcattggca gtaggcgatc ttgaatttca actaaaatgt atggaaaaat ttacagaaat
    24001 caagaactcg ggaaagacga ttttattcgt ttctcatgat atcagttcga ttcgtcgttt
    24061 ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg atacgatgga
    24121 agtaacgacg aattatgaaa actttttaaa gaaaaaatca gttaaaacag tggatcggaa
    24181 taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc gacgtgacga aagcggaatt
    24241 gttggataaa gacaaaaatc cggtggatat gctggaacaa ggagaaaaac tatacgttaa
    24301 agtaacttat gaagtaaaag atgatacaat caaaaatccg gttttaggaa ttgcgctacg
    24361 aacagtcgac aatttttatg tatgcggact taatactcta ctagatggta tcaagattcc
    24421 ttggaaaaaa ggagagaata tcttttatct ggagtatgaa aagttaggtt tgttagctgg
    24481 tgaatattat tttgacgttg cggtctttga agaaaatgca acggttcctt tggtatataa
    24541 gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt
    24601 agaccataaa tgggaagaag ggactcatag caatgaaata tgattttgaa atggacctag
    24661 atgaacaaag cagtgtggga aaaattgccg cacagatcaa accaggaagt aaagttttag
    24721 agtttggtcc cggaaacggc cgattaacga aacatttaat cggagcaaaa caatgtgaag
    24781 tgagcattgt tgagttagat aaggaattgt tcgattttgt cagcgaattt gctcaagatg
    24841 gattttatgg tgatatcgaa agttttgagt gggcaaacta ttatgctgga cagacatttg
    24901 attacgtctt gtttgcggat gttttagaac atttggtaga tcctggaaaa acattgaaga
    24961 aagtcagaga atttttgaat gaagaaggag agatcctgat tacattccct aacttggcgc
    25021 ataattctgt catgatcgat ttgttcaata accaattgcc gtgggcttct tatggtctcc
    25081 tagatgaaac ccataattct ttttatactc atgatggctt ccaaaaagtt tttgaaaaag
    25141 cgggattatt cattaatatt gaagattatt tgtacttagc tgtaggtgat acagaattaa
    25201 aatcaactta tgaagaattg ccggaagctg tacgctatga tttcaaaatg cgaccatttg
    25261 gtgaagtcta ccaatacttt ttctcattga tgaaacatcc agtagcacaa agttccattg
    25321 cagagccaca aaattctaat tatgtaaaag tgttagaagt aacccagcaa acgaaacaag
    25381 atgaaacaat ccaacaaata ccattcaata atttcacagg ggaaaatgag acactaagtt
    25441 tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa cctagcttta
    25501 tcgaattcag cgcagaagct gccggagaaa aactaacatt tatcgattcg aatgctgtag
    25561 tcaaaacagt aaatgattgt tatttgtttg atgggaaaga attaccagag ttcgttctaa
    25621 cggatatttc tgggaaagaa gtgactatcc attgtcacta tcgctttatc ggggaattga
    25681 caccaacaat gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc acaaagcagc
    25741 tttctgagaa aaatgacgaa ttagaaagag aaaatcatca tcttcttgag gaaaatacaa
    25801 gactagacca tacgttaaaa acaaccacaa atcgctattg cacattgttg gaaagtgatg
    25861 aatggacaat aaaacataga ctaggacgac gtaagaaaga aacttcgaaa aaaatccaag
    25921 aaaaagaact ttctatatgt attgacgaga aaatctggga tgcagaaaca aaaatattaa
    25981 aaatcatcgg atggggaatc gctaattcgg atcgccagcc cttgtcctat aagttatcag
    26041 ctgaccaatc gccatttttc gaagcaattc ctgtttcacg agaagaagtc aatcaagcag
    26101 aacagctcgc aaaaggaaca gaagcgggat ttgagttacg aatcctttgc gagcaagaac
    26161 gttcattctt agtggaagca gtagctcaaa atggccaatc atggatcatt gaaatgtaaa
    26221 taatgattaa cttataaaga taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt
    26281 atttatcgat caaatcactc gtgaacgggc cactggcgat ttgcttattg tgggctgggc
    26341 aattgatgaa gtgaccaaag aaatccctac gataaaggta gaaaaagaaa atgtgattgc
    26401 tgaagcaaca catgtcgtaa gattggatat caatcattta tataatcttg atgttaagac
    26461 ccaaagcggt tttaaggtac gtttatcagg gaaaatgaga ggaaaagcca tcttagactt
    26521 ccaaacagcg agacatcaaa atggcatagc tgtgaaatta aacggtaaat acccttatga
    26581 tgacggcatt gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta
    26641 tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat tgaagccagg
    26701 atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa ccagatttga aagagcaaag
    26761 gaaagaagca caaggatttg cgtaccgtcc attgatttcc atacttatgc ctgtctataa
    26821 cgttgaaatc aaatggctcg aaaaatgcat tgattcggta ctggatcaaa cgtatgatca
    26881 ttgggaacta tgtatcagtg atgatgcttc tactgatcca gctatccgaa agtgtctgga
    26941 atcctatcaa gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa atggacacat
    27001 cagtctggca actaattctg cattggaaat agcagaagga gaatttatag cactcttaga
    27061 taatgatgat gaattaccgc catttgcatt atatgaagtg gcaaaagtgc taaacgtaca
    27121 tccagaactg gatgtgatct acagtgacga agataaaatc gatgcagatg gcaatcgctt
    27181 tgatccgcat ttcaaagcag actggtcgcc tgatacattg atgggaaata attatatctc
    27241 tcacttaggc gtatatcgaa caagcattgt taaagaactt ggcggtttcc gtaaaggcta
    27301 tgaaggttct caagattatg atttggtcct tcgtgtgaca gaacaaatcc ctgcagatca
    27361 tatttaccac attgaccgag tcttgtatca ttggcggacg atcccaggat caactgcaag
    27421 taatggcgaa gccaaaagtt atatctatga ttccggagtg aaagcattga cagatgccct
    27481 gtctagaaga aacatcaaag gaagcgtacg tcctggcaga atttctggat tttatgagat
    27541 tgcttatgac gtcttgcaag aggatttggt cagtgtgatc attccaacga agaatggcta
    27601 tgaagatcta aaaacttgtg tggattcgat catcgaaaaa acgtcttatt caaattatga
    27661 aatcattatt gcggataacg gaagtacaga tccaaaaatg caagaattat ttgcagaata
    27721 caagcatcag ctgaaagacc gctttatcgt agaattgatt gacattccat tcaattattc
    27781 tcgaattaac aatttagcag cagaaaaagc aaacggcaaa tactttttat tcttgaataa
    27841 cgatacagaa gtgattgagc cagattggat gacggtgatg gtttcttatg cacagtttga
    27901 tcgaatcggc tgtgtaggag caaaactgtt ttatccagat gatacaacgc agcatgcagg
    27961 agtattatta ggaatcggtg gtgttgcagg gcatgcattg aataactatg atcgaacaca
    28021 ttgtggttat ttcggccgat tggtcattga cgtcaattac ttagcagtca ctgcagcttg
    28081 tatgatggtc aaggcagctg acttcaatgc tgttaacggg tttgatgaaa cattagaagt
    28141 agcattcaac gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta
    28201 tgcacatcaa gcagaattgt accatttcga atctaaatca agaggttatg aagatactcc
    28261 agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg caagataaat ggccagcata
    28321 tattgcgcat gatccatttt acaatgataa tttaacaaaa gaaggaatcg gcgacttttc
    28381 gttgagacca gattaaacta taataaaaag ctgttatgtt gtgattcgct cgcacataac
    28441 ggctttttat agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa aaataagtcg
    28501 aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa aattgctttt
    28561 caaatttttg tgaatttcgg cttatttaag aagaagttgc ttctgctccc accgtttatt
    28621 cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt cccacgctct tattcatgct
    28681 cagagcgcca atagatacaa aatcatagcc aaagtttcac gaaccctagc tgtcaaagaa
    28741 tcgatatttt attttaatat aggctatttt aaaataaatg gtttaaggta ttcctctaca
    28801 aaagccactc tatcaattat catgggttta aaataagaag tgatggagat aaccagattg
    28861 ttcttagcgt caatataaag aacatttcca ctattaccaa ttgctgaata tatttcgttc
    28921 tcagaatcta atacccacca taaatagcca tatctcatat tttgtaagtc ttctaaagca
    28981 ctggttctag gtttgaacat ctcgtttatc cacatgggtg gtacgatttg ttttccttta
    29041 tacatccctt tatttaggca taatacacca atttttgcca gatcacttgc cgataaacac
    29101 aaaccatatc ctgccgtccc gatatttagt ggatcacaaa accagacatt ggttttagga
    29161 gacttatcca ttgtaaactg cttatgttct tcagctgttt cagcataata attgacatgg
    29221 tttggtatcc ctataggatc aaatagatac ttattggcaa aatcaacagt tgttagctta
    29281 cttgtctgat acaacaagcc agttaatatg tgtaaacaga cagtttgata gttgaattcg
    29341 ccggttatac cttttcttcc tcccaataaa tctagtgatg tttttgtcca atcttcactt
    29401 gaacagactt ttgtccatgg atcgccttta catttgtagg gtgctttcat tgttagcaag
    29461 tgcttgattg taatagcgta aatagttttc tccccgcgtt tcactttata ctctggaaaa
    29521 taatctaata ctttatcatc gatacttttg attaatcctt gctctaaagc aatgccaatc
    29581 agcaaagata caatactttt cgttgctgac atgatatgac aagtatcgtc ttttttataa
    29641 ccattccatt catctgtata aacttctacc ccatccttat atgcgacaat ttgacatata
    29701 tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt tattcataag atattacctc
    29761 actttcttct ataagcataa tgtcattaaa aatcgatttc aattttttcg attcttcacg
    29821 taatacaggt attcttatgg actagacagc gctttgggaa gatggattct ctgaaaaata
    29881 taaaggttca ctttatgagt ggataaaaaa agcagcaaat acttcgactt ctttaattta
    29941 ccaacattag tgaaaatcga ttcatctgga gatttgtaat ttagagtcct tgtcttgatt
    30001 agaggtgtat aaagcttttt attagacatt tttaaattcc attacaattt gcttataaac
    30061 taggttttta aaggtgacta tggtaagata
//
LOCUS       DAZAFF010000029        29128 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000029 DAZAFF010000000
VERSION     DAZAFF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.029"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACG9BB_001206"
     CDS             complement(<1..1031)
                     /locus_tag="ACG9BB_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2837162.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACG9BB_001207"
     CDS             complement(1113..1802)
                     /locus_tag="ACG9BB_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN2837163.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACG9BB_001208"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACG9BB_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFN2837164.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACG9BB_001209"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACG9BB_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFN2837165.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACG9BB_001210"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACG9BB_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFN2837166.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACG9BB_001211"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACG9BB_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFN2837167.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACG9BB_001212"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACG9BB_001212"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFN2837168.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACG9BB_001213"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACG9BB_001213"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFN2837169.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKINDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACG9BB_001214"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACG9BB_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACG9BB_001215"
     CDS             complement(7636..8523)
                     /locus_tag="ACG9BB_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN2837170.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACG9BB_001216"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACG9BB_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFN2837171.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACG9BB_001217"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACG9BB_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFN2837172.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACG9BB_001218"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACG9BB_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN2837173.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACG9BB_001219"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACG9BB_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFN2837174.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACG9BB_001220"
     CDS             complement(10454..10957)
                     /locus_tag="ACG9BB_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFN2837175.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACG9BB_001221"
     CDS             11554..12519
                     /locus_tag="ACG9BB_001221"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN2837176.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACG9BB_001222"
     CDS             complement(12618..13790)
                     /locus_tag="ACG9BB_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2837177.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACG9BB_001223"
     CDS             complement(13777..14922)
                     /locus_tag="ACG9BB_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2837178.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACG9BB_001224"
     CDS             complement(14936..15868)
                     /locus_tag="ACG9BB_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2837179.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACG9BB_001225"
     CDS             15985..17064
                     /locus_tag="ACG9BB_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN2837180.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="ACG9BB_001226"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="ACG9BB_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17789..18016)
                     /locus_tag="ACG9BB_001227"
     CDS             complement(17789..18016)
                     /locus_tag="ACG9BB_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN2837181.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="ACG9BB_001228"
     CDS             complement(18119..20707)
                     /locus_tag="ACG9BB_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFN2837182.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="ACG9BB_001229"
     CDS             complement(20727..21386)
                     /locus_tag="ACG9BB_001229"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN2837183.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACG9BB_001230"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACG9BB_001230"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFN2837184.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="ACG9BB_001231"
     CDS             complement(22159..23025)
                     /locus_tag="ACG9BB_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFN2837185.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="ACG9BB_001232"
     CDS             23134..23775
                     /locus_tag="ACG9BB_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFN2837186.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACG9BB_001233"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACG9BB_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFN2837187.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="ACG9BB_001234"
     CDS             complement(25216..26115)
                     /locus_tag="ACG9BB_001234"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN2837188.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="ACG9BB_001235"
     CDS             complement(26117..26914)
                     /locus_tag="ACG9BB_001235"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFN2837189.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="ACG9BB_001236"
     CDS             complement(26892..27596)
                     /locus_tag="ACG9BB_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFN2837190.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="ACG9BB_001237"
     CDS             27727..28302
                     /locus_tag="ACG9BB_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFN2837191.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="ACG9BB_001238"
     CDS             28420..29097
                     /locus_tag="ACG9BB_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFN2837192.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8574 a         6169 c         5236 g         9149 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactac acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaaataa
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aatgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actatttaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
    29101 aggctctttg tcaaatgatg ttggtaaa
//
LOCUS       DAZAFF010000030        27657 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000030 DAZAFF010000000
VERSION     DAZAFF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.030"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..1894
                     /gene="yfcC"
                     /locus_tag="ACG9BB_001239"
     CDS             155..1894
                     /gene="yfcC"
                     /locus_tag="ACG9BB_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFN2837193.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2051..3235
                     /gene="iadA"
                     /locus_tag="ACG9BB_001240"
     CDS             2051..3235
                     /gene="iadA"
                     /locus_tag="ACG9BB_001240"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFN2837194.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3346..3549
                     /locus_tag="ACG9BB_001241"
     CDS             3346..3549
                     /locus_tag="ACG9BB_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFN2837195.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3650..4312)
                     /locus_tag="ACG9BB_001242"
     CDS             complement(3650..4312)
                     /locus_tag="ACG9BB_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2837196.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4325..5827)
                     /locus_tag="ACG9BB_001243"
     CDS             complement(4325..5827)
                     /locus_tag="ACG9BB_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2837197.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6375..8054
                     /locus_tag="ACG9BB_001244"
     CDS             6375..8054
                     /locus_tag="ACG9BB_001244"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN2837198.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8181..8660
                     /locus_tag="ACG9BB_001245"
     CDS             8181..8660
                     /locus_tag="ACG9BB_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFN2837199.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8879..9559
                     /locus_tag="ACG9BB_001246"
     CDS             8879..9559
                     /locus_tag="ACG9BB_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFN2837200.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9567..10898
                     /locus_tag="ACG9BB_001247"
     CDS             9567..10898
                     /locus_tag="ACG9BB_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFN2837201.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10913..11875
                     /locus_tag="ACG9BB_001248"
     CDS             10913..11875
                     /locus_tag="ACG9BB_001248"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFN2837202.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11986..12324
                     /locus_tag="ACG9BB_001249"
     CDS             11986..12324
                     /locus_tag="ACG9BB_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFN2837203.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12403..13110
                     /locus_tag="ACG9BB_001250"
     CDS             12403..13110
                     /locus_tag="ACG9BB_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFN2837204.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13213..14694)
                     /locus_tag="ACG9BB_001251"
     CDS             complement(13213..14694)
                     /locus_tag="ACG9BB_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2837205.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            15003..15539
                     /locus_tag="ACG9BB_001252"
     CDS             15003..15539
                     /locus_tag="ACG9BB_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2837206.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15617..16987
                     /locus_tag="ACG9BB_001253"
     CDS             15617..16987
                     /locus_tag="ACG9BB_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFN2837207.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17054..17389
                     /locus_tag="ACG9BB_001254"
     CDS             17054..17389
                     /locus_tag="ACG9BB_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837208.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17470..18327)
                     /locus_tag="ACG9BB_001255"
     CDS             complement(17470..18327)
                     /locus_tag="ACG9BB_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2837209.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18433..18948
                     /gene="tadA"
                     /locus_tag="ACG9BB_001256"
     CDS             18433..18948
                     /gene="tadA"
                     /locus_tag="ACG9BB_001256"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFN2837210.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            18971..19055
                     /gene="ffs"
                     /locus_tag="ACG9BB_001257"
     ncRNA           18971..19055
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG9BB_001257"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19042..19296
                     /locus_tag="ACG9BB_001258"
     CDS             19042..19296
                     /locus_tag="ACG9BB_001258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837211.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            19265..20650
                     /locus_tag="ACG9BB_001259"
     CDS             19265..20650
                     /locus_tag="ACG9BB_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFN2837212.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20687..21364
                     /locus_tag="ACG9BB_001260"
     CDS             20687..21364
                     /locus_tag="ACG9BB_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFN2837213.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21372..22136
                     /locus_tag="ACG9BB_001261"
     CDS             21372..22136
                     /locus_tag="ACG9BB_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFN2837214.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22138..22797
                     /locus_tag="ACG9BB_001262"
     CDS             22138..22797
                     /locus_tag="ACG9BB_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFN2837215.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(22827..23336)
                     /locus_tag="ACG9BB_001263"
     CDS             complement(22827..23336)
                     /locus_tag="ACG9BB_001263"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFN2837216.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23448..24446)
                     /locus_tag="ACG9BB_001264"
     CDS             complement(23448..24446)
                     /locus_tag="ACG9BB_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN2837217.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            24647..25231
                     /locus_tag="ACG9BB_001265"
     CDS             24647..25231
                     /locus_tag="ACG9BB_001265"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN2837218.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25308..26483)
                     /locus_tag="ACG9BB_001266"
     CDS             complement(25308..26483)
                     /locus_tag="ACG9BB_001266"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFN2837219.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            26565..26945
                     /locus_tag="ACG9BB_001267"
     CDS             26565..26945
                     /locus_tag="ACG9BB_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFN2837220.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27019..27381
                     /locus_tag="ACG9BB_001268"
     CDS             27019..27381
                     /locus_tag="ACG9BB_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837221.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         8979 a         4676 c         5771 g         8231 t
ORIGIN      
        1 aaaataatca tacataggag aaaaaaaaca aaatagaaaa aagtgatttt ctaataaatt
       61 attcatgtgg aaatgtcatt tttactgtat aattcatttg attatccatt ttttattcat
      121 caaaattact gaaaaaagaa aggaagaaat aaatttgaag aaaacaaatt cttcaggaaa
      181 accgaaacgc gacttcacta aattatcgac accccatacg tatgtcatta tttttggtgt
      241 agtcatcttt gcatggatac tgacttttgt cgttccagca ggaaagttca gcacgcaaga
      301 tattgaatac aaagatgcaa atggcgaaac aagtactcgt actgttttac gtcaagattc
      361 cttccgttat gcttacgagc tggataagtc gtatgtgttt gatcaattag aagaactgca
      421 agatcatcca gcagaacgtg aaaaactgga tgttccggaa aaaggattgg aaaaagtcat
      481 tgctgacgga gaaaaaaatc ttacgcagga aaaactagat gaaatctcct tgacagatga
      541 tgtgctttac gatcagtacg gcgaaaatat ctatgatact tctaaaaaat tgcataaaac
      601 agccaaaata tggggaacag atgattttgg cggctttggt ttcttaaatt ttgtttttga
      661 aggactagta tctggagata agtatggatc tgctgtaggg atcgctgcat tgatccttgt
      721 tgttggaggt gcttttggta ttattatgcg taccggagcg atcgacgcag gtatctatgc
      781 gtttatcagt aaaactaagg gactggagcg tttagcttta ccgctattat tctttgcttt
      841 ttcattcggt ggcgctacat tcgggatggc agaagaggta attccattct caatggtgat
      901 ggttccattt gtcattgcgt taggatatga ctcgatcgta gcagtaaccg tgacttatgt
      961 cgcatctcaa gtcggaaatg cgacctcttg gatgagcccg tttagcgtag cggtggctca
     1021 agggattgct ggaatccccg tgttgtctgg tgcgacattc cgattgatta tgtgggtagt
     1081 tgttactgca ttagccgctg gttatatgat gatatatgca gaaaaaatcc gtaaaaaacc
     1141 agaaagttcg ttgacttaca aatcagatga ttatttccgt tctcatatca aaaagacatc
     1201 tgatgaaaat aaaaaattca tgcttgggca taaattgatc ttattagaga tgttagttgt
     1261 ccttgtctgg atcgtttggg gcgtgactca aaaaggctat tacattccag aaatcgcttc
     1321 tcaattcttt gtgatgggat tagttgctgg aatcattgct gttcttttca aattagatgg
     1381 aatgaaagtc aatgacatcg cttcttcttt ccaatctggt gcagctgatt tagctggtac
     1441 agcaatcgta gtaggtatgg ccaaagggat tcttcttgtc ttgggaggat ctgatgcatc
     1501 tgttccttca gctttgaata cgattttgca tgggatcggg acggcgttga ccggtgttcc
     1561 agctgtcatt ggtgcttggg cgatgtacat cttccaaagt ttgttcaact tggttgttac
     1621 atcaaattca ggacaagcag ctttgactat gccaatcatg gctccactgg cagatttagt
     1681 aggtgtatcc agacaagtag cagttcttgc ttatcagctt ggttcaggtt ttatggacgc
     1741 ttttacaccc gtatcagcca gcttgattgg tgtgctaggt gttgcacgga tcgaatgggc
     1801 aaaatgggca agattccaaa tcaagatgca aggattcctg tttgttttag gaactatttt
     1861 catcatgatt gccattgcaa taggtctgca gtagtactgg agacctgtaa ataaaaaagt
     1921 aatccaaaca gtagataagg actccaagaa ccgtcatttt acggtaaaat caaaggagta
     1981 aagatcctat cgtttggatt attttttaag atcctatcat cagttatact taaagaaaga
     2041 ggaaaaatgt atgaaaatca ttcgacaaat tgaagtgttt gcaccagatt acttaggaaa
     2101 aatggatgta ctgattgccg gtgggaagat tttggctgta gaagaacagc tagaaggcgg
     2161 ctacgaagga gtggaagtag aagaactttc aggtgaggga aaagtactaa ccccgggttt
     2221 cattgactgt catttccata ttttaggtgg aggaggagaa ggcggttatc aaaatcgaac
     2281 gccagaagtc acattgagcc aattgactac agctggtgta acgacagttg tcggttgttt
     2341 gggaacagac ggagaaggta gagatatgac tgcgttgatc agcaaagcga aaggtctgga
     2401 agcagaagga atctccactt atgtatacga aggttcgtac cgattgccgg taaaaacagt
     2461 aacagattct atcatcaaag actttttgac aattgataaa attatcggaa tcggagaaat
     2521 cgcagtatcc gatcaccgtt cttctcagcc aagttttgaa gagttcgctc gtgcagtagc
     2581 agatgctcgt gtaggaggca tgttatccgg aaaagctggg atcatcaatg tccacttagg
     2641 cggaggaaaa agaaagctag agctattgac acgaatcgta gaagaaacag aaattccgat
     2701 cacacaattt ttgcctactc atgcaaatcg cacaccagaa ttatttgaag cttgcgtggc
     2761 atttgccaaa cgtggtggca cgattgactt cactgctagc gaagatcctg atttttggga
     2821 gaagacagat ggtgaagttc gcttcagcaa agcactgaaa cgattgatcg aggaagatgt
     2881 ttctttagat aatttcacga tgacctcaga tggacaagga agcttgcctt attttgatga
     2941 aaacaatcat ttcctagggt taggagtagg aagtgccaag gcattgctag tagggatcaa
     3001 agaagctgtg caaaaagaaa gtatcccttt ggaaatcgcc ttacgagcta taaccagtaa
     3061 tccagcacgt atattgaaat tggataaaaa aggaaaaatc gaaattggtg ctgatgcaga
     3121 tctgtgtatc ttggataaag aaacgcttga tattgataca gttatcgcta aaggagaaat
     3181 aatggtccaa gaaaaagaag tcaaagtatg ggggactttc gaaaaaagtt tttaatcaac
     3241 agctctaact atctttttta gaaaacgagc gttctgtttg tctgtatctg ttgttgatgt
     3301 taccctgaag gtagatcaat aaacaaacta agaggaggaa gcacaatggc agatttaaaa
     3361 ggacgttcta aagatatgaa agataaagta gtcggtgaag gaaaagaagc atacggcaaa
     3421 gcaacagacg acaagagcaa agaaacagaa gggaaagcac aatcactagg tgctgacatc
     3481 aaagaaaaag ctcgaaattt aggcgacgaa gtcaaagaca agttttctcg aaaagacgaa
     3541 aaaaaataaa aaccacttat tactaattga atcaagcaaa taaaaaatcc gaaccatatc
     3601 tgcgagacag atatggttcg gattttttat ttgatcatta tcctgttttt taaacttcga
     3661 ttttttgaaa ttttttattt tttagttcaa agacttcatc acagcttttt gctacttcaa
     3721 tctcatgagt cacgatgata atacagcgat tttgttcatg ggcgatttct tggaaaagtt
     3781 tgacgatatc ttgtgaagtt gtttcatcta gatttccagt aggctcatcc gctacgatta
     3841 gttgatgttc acagcagata gcacgaacga tagcgactcg ctgctgctga cctcccgaaa
     3901 gtttggtcac ctgttttttt gctaatattt catcaattcc tacacgagcc agtcgatcta
     3961 aagcaacttg tcgataattc tcttgcttgg tcttagtgat ttccattgct gctatgacgt
     4021 tgtctagagc cgataaataa ggaagcaagt tgtaagcttg gaaaataatc gagacatcat
     4081 ttttacgata acttcttaac ccgattttgt tcagtgaacg atcttgatag tatacctttc
     4141 cttcttttgg tgcatctagt ccagaaataa gcgaaagaaa agtcgttttt ccagaaccac
     4201 ttgctcctaa aatagcgtac atttttccag cttcgaaatt cagttctaca tctttataaa
     4261 gcgcatcttg cggatcttga taccaaaatc ccaattgttc tgtccgtaac atcaaattcc
     4321 tcctttagtt taataggatt tttcttgggt ttaatcttaa tatccctata gaagctaaaa
     4381 agacagaaaa cagactgatt ccaaatgcta ctccggctaa tagtcccaat tgtgcaggtt
     4441 tgacattgat ttccaattcg ctgacttgat cagaaacagc aaatggatta ttcgtggttc
     4501 cttgttgacc atcgcgttga ccgccttgtt cttgtctgtt acctggcatt tgtccaccgc
     4561 caggagcttg ctggttttgt gtcgtggttt ctgtctgttg gtcaagtagc tgctgtccca
     4621 cggcattcgc gacgacgttt ccgctaagcg aagcaactcc taatgcaaga atcatacaaa
     4681 tagccacttc ggtaaacaat tggagaatga ttttacttct ggattctcct aaagatagca
     4741 agacgcctaa ctcatgccga cgctcccgaa tggtaatcat aatgatcaga gtaagtatga
     4801 taataccggc tacagccact agtaaaacaa catttttaga aaaacttgcc acgttgttta
     4861 aaggttccaa cattgactga tacatggaat catttgattg taagctaaat gtatctgtgt
     4921 cgatcagctt ctcagcttct ttgagaaact catccatttt atctggatca gatagagtgt
     4981 aaatagcaga atctatcgtg ttatcgtcag atgtcccatt tagttcattt gcaaaagtgt
     5041 aagaagtgta aagtgtattc gacggattca tgaagttgaa tttcataccc atactgccgc
     5101 ttgtttcgct ggattcataa attcctttga tcgtcatttc ataagtggta tcttcatctt
     5161 cagtactcgt gattgtgaaa gtatctccga ctgccaggtc atttgtttct gctagagtag
     5221 aatcaatgac tacattgttt gtgcctttgt cgctgtcatc tattccttca ccatccgtga
     5281 tttttgctgt tccatcagaa aaattactat tttgtgcagt tgctgacaca ccactgattt
     5341 gaaagtctgc ttgagtcatt tgcggattcc cgcccattcc tccagcaggt tcaccgcctg
     5401 catcagtagt ttctgaactg tcttcacttg aatcagaact agtgatggca gttattccat
     5461 cttttgccat agctgaggcg gaactttcaa aggaataact ctttacatta tctaattcag
     5521 ctattttttc cgcgtccgtt aaggaaactg gtgtaagaga aaaactgcct ggatctggac
     5581 gttcttcagt ggtagaaccg cttgaagaag tttgttcctg ttttttaaat gaagcttctc
     5641 gattggcact taaagtcact gttgctccaa cttctttttg tgcttgattc gctgcttctt
     5701 ctgcagcgct tcgaatcgtg aggccagcta gcacaaaaat aagaatggct gtgaacacgg
     5761 caaacaataa caatgaacgt ccccatttta cttttgtact tttccatgca cgtttcataa
     5821 aattcatatt ttgcatccct cctagtagat gcatttgata atagacaacc tatcttaaag
     5881 ctagcttaaa tcttatgctg tttgcacacc atcaacatga ggttctgtat atataaaaag
     5941 tcactatagt tattgcaaaa aatgtggtaa aatgaaaaaa gtatgtttca aaccgacgaa
     6001 gaagaaatga aagaggaata gcaaaagaaa tcctagatgc tacagaggga tttttacgtg
     6061 catagttgtt ctaacaattg ctgatactag cgatttagtt tatttgttgt ataataatgg
     6121 ggaacagctg aatacttata taagtattct ggctatagcg tttcgcaaaa gatccatttt
     6181 gttaatgaag tatatccaaa ataaaatgcc catccaactg tcagaacgcg gatgtgctgg
     6241 taattttcct gctgattctc cataagttga gaaatagtga ttttgcatat gcttcattgg
     6301 aatggctcat atatttagat aaagaagaaa atcgacatct ttaaacttgg atgtcataag
     6361 aagaggtgaa gtttgtgagt accattaaaa ttatcccatt aggcggcgtt cgtgaaaatg
     6421 gaaaaaacat gtatgtcgca gaagtgggcg aggatatttt tattttagat tgtgggctaa
     6481 aatatcctga aaatgagttg ttggggatcg acgtcgtaat ccctgatttt acgtatttag
     6541 aagaaaacat tgatcgtgta gctggtgtct ttttaacaca tggacatgca gatgcgatcg
     6601 gggcacttcc ttacttgttg tcaaaaattt ctgtaccagt attcgggaca gagttgacga
     6661 tcgagttagc aaaattgaat gtcagtcgta atgaagcagc caaaggattt aaggatttcc
     6721 atgtggtaga tgagcataca gaaatagact ttggtgaaac agtagtgagc tttttccgta
     6781 ccactcatac gataccagat tcagtgggga tcaatctgaa aacaaaagaa ggtagtatcg
     6841 tttatacggg agattttaaa tttgaccaaa cggctatccc aatgtatcaa acagattatg
     6901 cacgattggc ggaaatcggc aaagaaggag tcttagcctt actaagtgaa tcggcaaatg
     6961 cagaaaatcc tcagcctgta gcttctgaag cccaaatagc agatgaagta tttgatacga
     7021 tcaaatattg ggaagggcgt atcatcgtcg cttgtgtagc aagtaatttg caacgagtgc
     7081 aacaagtatt ggatgcagca gaaaaagcac aacgaaaagt tgtcttgact ggtcaagatt
     7141 tcgaacgaat tattcgcaca gcgatgcgtt tagaaaaact tcaattacca agtgaagatc
     7201 tgcttgttac gttgaaagac atgaaaaact atgatccaga agaactgatc attttagaaa
     7261 ctggccgaat gggtgaaccc atcaagtctc tgcaaaaaat ggcaaacggt acacaccgta
     7321 atgtcaagat tgaagaagga gatttggttt atatcactac tacgccaagc atcgcgatgg
     7381 aaaccatcgt ggcgaaaacg gaagatatca tttatcgagc tggtggaaca gtgaagttga
     7441 tctctgagaa tatgcgcgtt tctggccatg ccaatcctaa tgacttgcaa ttaatgatca
     7501 atttgatgaa accaacatat tttgtgccag ttcaaggaga gtatcgtgaa ttagctgctc
     7561 atgcagattt ggctcatgca gtagggatgc catacaaaaa tatctatatt actggacgcg
     7621 gagacgtgct ggaatataag aacaagcgta tgagtgttgc aggaactaca agtgcagaaa
     7681 atgtaatgat tgatggatta ggcataggtg atatcggaaa cattgtttta cgtgatcgaa
     7741 aaatcttgtc agaagacggg attttcgtag cggttgtcac tatcagtcgg cgagaaaaga
     7801 aaatcatttc aaaacctcaa attacttcac ggggattcgt ttacgtaaaa gctagccgtg
     7861 atctgatgaa agaaagcagc aatatgattg aaacaatcgt tgagaaacat ttagaaagca
     7921 atgattttga gtggagcaaa ttaaagcaag acatccgaga tcaattaggc cgttacttat
     7981 ttgaacaaac aaaacgccgc ccagtgatct tgccagtcat tatggaagct actcaacgtc
     8041 gaaataaaaa ataaacaaaa agagtaagat gaacctctct atagagtgct cgtcttgctc
     8101 tttttttgcg aaaaacaaat aatttttgaa tacagcatcc ctcgctttat aataaacgat
     8161 agaataaagg gagtgaatca atgatgagtc aaacaaaagt cgaacgttat ttagaagaac
     8221 aaaagatcag ctatcaacca cttgatttta gtcatgtggt agaagaatct ttcacagaag
     8281 aactacaaaa aagagggatt gatccagctt tgatatgtaa aactctcgta gtgaaaggaa
     8341 acaaaactgg ggtgatcatc gcattgattc ctttgaacga tcacttagat tacaaaaaaa
     8401 cgagacaagt gacgaaagat cgtaaagttg gttttcctgg aatggatttt gtattaatgc
     8461 atacaggata tccccacgga gcaaacaccc ctattggaat aaaactcgca catcctgatt
     8521 attgtttttt agcagacagt tctttaaaag aaaaagaaga agtaattgtg tcaagcggtg
     8581 aaatcgggag aagccttcgg ataaaagtag ccgatttaga aaagatcgtc catcctattt
     8641 acgctgattt aattagttaa gggacgagaa gagaagctaa gcagagcaga gacctcaaac
     8701 ttgagattat atctgcttgg tacttggtgc atcctgtatt atctgttaca attgctttgt
     8761 acatactgta ctatcaatcc aatgaaaata tatttagcag ctatattttt tcattgatgt
     8821 gcaagtgatg aaatcgtttt taataaaaaa gatacagaga aaacgcgagg tgtaaaatat
     8881 gaaaattacg tatcatggac attcctgttt aacaattgaa atggaaaatg gccaacgcgt
     8941 gatgattgac ccgtttatta caggaaatcc attgacagac ttaactgcag aagaagtaaa
     9001 gacagattgg ttgctgatta ctcatggtca tagtgaccac ataggagata tgattcctat
     9061 tgcaaaagaa aatcaagcca caattatcag tattgttgag attgctgatt atgcgaacag
     9121 cagaggagta gattcattcg gtatgaatat cggtggaaaa catgcgtttc cattcggcgt
     9181 ggtaaaattc gtacatgcac agcatagttc aagctatgaa gtagacggta tcgttcagta
     9241 tatgggcgag cctagcggaa ttattattca agcagaagga aaaacgattt atcatgcagg
     9301 ggatactgct tatttcagtg atctaggatt gcttgcagaa gaattcgata ttgatgtcgc
     9361 tttcttgccg attggcgata actatacgat ggggccagaa gatgcagcaa aggctgcgca
     9421 aacaattcgt gccaagaaag tagttccaat ccattacaat actttccctg tcattgaaca
     9481 agatcccgaa tcttttgtag agttactgga agatagtgaa ggaaaaataa tgagcgtctc
     9541 ggaaacgctg gaagtgtagg cagaaaatgg cgacaaaaca tgatttgatt ttagaatata
     9601 ttgaaagttt acctgttggc aatcgaattt ctgtaagaag catcgcaaaa gaattgaatg
     9661 tcagtgaagg aacagcgtac cgtgcaatca aagatgcaga aaatgtcgga ctagtttcaa
     9721 caattcaacg agtagggacc atccgtatcg aacgcaaatt aaaaaaacat attgaacgtt
     9781 tgacatttgg cgaggtcgtc aggatcattg aaggggacgt tttaggcggg gcggcaggat
     9841 tagacaaggt cttgaataaa ttcgtcatcg gcgcgatgac taaacatgcc atggaacgct
     9901 atattacgcc aggatcacta atgatcgtcg gcaaccgggc ggaagtccaa aaattagcat
     9961 tggaagatgg cgccgcagta ttgatcacag gaggattcaa tacgactgct gagattgcaa
    10021 agctagctga tgatcttgaa atgccgatct tacgcacctc ttatgataca tttacagtcg
    10081 caaccatgat caaccgagcg ttaagcgacc aattgatcaa aaaagatatc atgcttgtca
    10141 gtgatatcta tatgccagct gaaaagactc attatttgca gcagacagat acgatttctg
    10201 attatcagcg tttgtcagaa gaaacgcagc attcgcgttt tccagtcgtc aaccgccatc
    10261 atcgtttgat ggggattgtt acggcaaaag atgttttagg gaaatcgcca aaccagatca
    10321 tcgatcgggt gatgacgaaa gaaccaatca gtgtaaagaa aacaatgagt attgcctctg
    10381 ttagccatca aatgatctgg gatgggctag aagtcatgcc ggtcgtttcc gatgacttga
    10441 cattggaagg cttgattacc cggcaagatg taatgaaagc gatgcagctt gtccagcgac
    10501 aaccgcagat tgcagatacg atttcggatc agatatctgg aaatatccat acgattgata
    10561 ctgaccgtga aggaaatcgt ttggaaaacc ctaagttcaa attcgtagta gcaccacaaa
    10621 tggtcaatgg cgttggaacc atttcttttg gcgtacttag cgaaatcatt tctgatgtcg
    10681 cacaaaaaac aatggtgatg aaccaaaaac gaaatatctt gattgaacaa gtgaacctac
    10741 attatttgcg gctgatccaa ttggaaagcg aattagacat tcgtccacgt gtattggaaa
    10801 tcggcagacg ttccgctaaa ctggatatcg aagtgtttat tgaaaacacc atcgtttcca
    10861 aagcaatcgt tgtttgtcaa gtaatggaac gttcttagaa agcaggagtg aaatgacagt
    10921 acagcaagaa atagtagata caataaaaaa atacgataaa atcattatcc acaggcattt
    10981 gcgccctgat cctgatgcat taggatcaca agtaggatta gccgaactat tacgggaaag
    11041 tttcccagaa aaagaaatac gtcaagtagg tgaaacgatt gaagggcttc gctttttagc
    11101 agaaatgcaa gaagttgaag atgtcttcta ccaaggagcg ctagtcatcg tgactgatac
    11161 agctaattca cccagaatca gtgatcaaag atatcagttg gcagaaaaac tgatcaaaat
    11221 cgaccatcat cccaatgatg acccttatgg agatcttgtg tgggtaaata caaaagcaag
    11281 tagctgtagt gagatgattg cagaatttgc tttgatgttt cctgaatctt taaagatgaa
    11341 cgttaaggct gcacggttac tttatgcagg aatcgtcgga gatacagggc gctttttgta
    11401 tccctccacg acgtcccgta cattagagat tgcttcgata ttgagaaatt atccatttga
    11461 tgcaagtgct ttgaacagag agattgaaca aatgccgatg aaagtcgcta aactttccgg
    11521 ttatctttat caaaatatcc aggtggatga aaatggtgca ggaagaataa tccttccgca
    11581 agaaattctt gataaatatg gaatcgatga ttcagagaca gcttcgatcg tttcacttcc
    11641 tggagtgatt gacgaagtgc tggcttgggg gatttttgtt cagcagcctt cagggtatta
    11701 ccgcgtacgt ttacgttcta aggggccgat catcaatgaa ttggctaaac gccatcatgg
    11761 aggaggccat ccattagcaa gtggtgcgaa tgcaaaagat ttagcagaag tagaggaaat
    11821 ctataaagag atccaagata tatgccaatc gtataagagc agaaaaacag aatgatactt
    11881 cgttcgaaaa cgtgcctttt gtagtaaaaa tcacaaatgt atcttttcaa gatcttcttt
    11941 ttccgttaca atagtgctca ctaaagattt taaaggagaa agaagatgac attaccaaat
    12001 tgcccagaat gtggctcaga gtacacatat gaagatcgag gcttgttgat ttgcccagaa
    12061 tgcgggaacg aatggaacaa agaagaacaa gaaactgtca tagaagacgg attagtggta
    12121 ttagatgcaa atggaaaccg tctgttagag ggcgatagcg tgacagtgat taaagacttg
    12181 aaagtaaaag gtgcgtcagg tgccattaaa caaggaacaa aagtcaagaa catccgttta
    12241 gtagaaggcg atcataatat cgattgcaag gtagaaggat ttggaccgat gaaactgaaa
    12301 tcggaatttg tgaaaaagaa ttaaagaata ttcaaggtct tttcacattc ttttcctact
    12361 actaatgata aaataaagta aacgaaaagg agtgaaacga aaatgtatgg ttatggaatg
    12421 tatggttttt tagatccgac atttcttttg gtcattcttg gattagttat ttccggaatt
    12481 gcgtcagcgt acgtcaatag tactttccga aaatacgatc aagtgagaag caaaaacaat
    12541 gtgactggta cacaagcagc acaatatata ttacaaagtc aacagataaa taatgtcggt
    12601 gttcaacaaa ttgccggaga tctcacagat aactacaatt caggtaataa gatgttgagt
    12661 ttgtctcaag caactgcaca gtctacttca gttgctgcga taggtgtagc agctcatgaa
    12721 tgcggacatg cggtacaaga tgcttcgggt tatgtcccat tgaagattcg tgcagcactt
    12781 gttccagcag ctaactttgg ttcgatgatt tcgttcccct tgattatggt aggtgtattg
    12841 cttagctgga actctacgtt aatcaatatc gggattttcg ctttttcttt agcattactt
    12901 ttccaattgg tgacattacc agtcgaattt aacgcgtctc ggcgagcaat ccaaattttg
    12961 agtgaaggcg gactactgac ggaagaagaa gtaccgatgg caagacacgt attatttgca
    13021 gcagcactga cttatgttgc agcagcgctt tcgacattcc tgcagctgtt gcgcttgatc
    13081 ttattgttcg gcggcaaccg aagagattaa aataaagaag ttgtgaaatc agcgctttga
    13141 tctgagctaa taccgagagt tagctgattg aataccattt atttggaata agaggatgtg
    13201 acaagaattt tgtcacatcc tctttgtttt gttctcataa ccttttttca tatacagttc
    13261 atgaaccgct tttattaact tgccaaataa cactgtattg tgcgttgtat ccagatagaa
    13321 agtctccgtt tcatctgaaa ttttctcatg ctgatctgta atgatcaaat cagcatgttc
    13381 tgaatatgcg gtaattttaa cagcatcttt gcgaaataac tgctgcaaat ggctttgaag
    13441 atgataattt cctgtaaact gtttggttat ctgcaaatgg atgagtaact gctctttcgt
    13501 ttctgtttgt agtaagtgtt ggatcaattg actaaggtat tgactgattt ggtttctttc
    13561 cccatttttt gaaaaaattg tatcgatttt ttctcttaat ttcttttcct gtataaaaga
    13621 actagaatga ttggacgtgg cttctggaaa gcaaagtgta gaaaaactac tgctggattc
    13681 acccaacagt tcgaaaaaaa caactgccaa cacaagaaaa taaaaataaa ttggtatttt
    13741 attttctaaa tggttgagag gaaactgttg agttaagtaa gtcatcaatt gaccgacttg
    13801 tcgacaaata aggtttttgc ttgtggaaaa taaaaaacca atctcctgtt tttgtcgaaa
    13861 agagatttga tttggaaaaa aacaatagaa aaaataatta aagtaggctc tctctgtttc
    13921 tgtctcttca caagggcgac tcactgaaaa agaagtaaag tattctgaat aatcatgtgt
    13981 atgctgcaat aagtccatca attcagtgat ttcagaagaa gtttttggaa aatatgcctg
    14041 ttgtttgttt cgtaatatga ataaagttaa cagcaagtag atattttttt cttgttttga
    14101 aattttttct atttgctcat ctgtactgtt catttttagt gcatttaatt caagtgagga
    14161 aaatggccaa tcgttacctc caaatgtatc tgaaagaaaa aagtaaagat agagacggat
    14221 agataactca tttccttcaa gagtacagca attttttaca gataggatcc gtaaatggaa
    14281 ttttattaag gaatgattga gctgttttgt cagtttttcg agataggagg gcgatatata
    14341 aagttgttca agtaaatatt tttttggtac agcagtttgt ggataattga ttagtaacat
    14401 caaatagtta aaacgattgg attcttttag gtagcataat tttaacttag aaaaaacagc
    14461 taacttttta gtttgatcgg tttctttggt ttggatctct cctttatgat tagaataaat
    14521 aaattcatca atcaataaaa attgcttgag tgatacattt atttttcgga tcagttcttt
    14581 gattgtccgt tttggaatat gtaagtcatt ggataattct tgtaaagaac aattattttt
    14641 ttgaatgacc atttgtagaa caagtaactc tttttttctt gcttcaatta gcataaatac
    14701 ctccttttta ttctgttttt gtatatagtt agacaaaaac tgtgagtgga aagtcatttc
    14761 agtctgtgtt attctcacag agtgtcgcct tcttttttat ataacgttat ttaatttttt
    14821 tgttaccttg tttattctat tgtttatttc ctagggtatt ttgtcaagat attcttgttt
    14881 ttaaggcgga aaaattgatt tttaatggat ataactaaat atgttttgat aattatcaat
    14941 aatattttta gaattattta tgttatttat ttatgggtag atgaaaagag acggaggtaa
    15001 gaatgatggg gagagtaaca aattatagta aagaatattt gctgttcaaa agtatggtat
    15061 acgtggaaga atatggaatg aagtcaatta ctgcaagaga gttagcagag ttctgcggtt
    15121 gttctacata cccgatttat acacatttca aatcaatcag cgggttaaaa gagaagatat
    15181 tagaagaaat cactgtttat tttgaaaact acttagctga atgcagttcc gatgatgttt
    15241 taagcattgt ctacttattg aaagattttt tttctatcca tgaaagtatt cgtgaaatca
    15301 ttgcaaaatc tgatttagac atggattcgc tatttaagcg gccatttttc tcatatgtca
    15361 aaggtcacac gaagatcaaa gaagaaaagt cgcttgaatt ggtatggctt aatgcattgg
    15421 gaagtttgcg ctcagatgat tcttctcaag attttcttga aatgatgaac gctgtttggg
    15481 ataaaaacga ggtgacggat gaatacttgg aaaatatctt aaaggaacag tcaccttagg
    15541 cagcaacatg aaaaagtaga tcagccacct ttatttttcg taaaaaagat tgtaaaatgt
    15601 ctaacgaaac attggaatga aggaggaagc gacaacgtta ccacagcaat ttatcaaaaa
    15661 atatcgtctt cttttaggcg aagaagcatc cgattttttt tctgcgcttg aacaaggaag
    15721 cgtgaaaaaa ggatttcggt ggaatccact aaaaccggca ggattggaca tggtccaaac
    15781 ttaccatagc gaagaattac aacccgctcc ctacagcaat gagggatttc ttggcactgt
    15841 caatggaaag tctttcctcc atcaagctgg gtatgaatat agccaagaac caagtgcaat
    15901 gatcgttgga acagctgctg cagcaaaacc cggtgaaaaa gttttggatt tgtgtgcggc
    15961 tcccggtggt aaatcaaccc aattagctgc tcaaatgaag gggaaagggt tattagtgac
    16021 gaatgagatc tttccgaaac gtgcgaagat tttatcagaa aatatagaac gatggggagt
    16081 ttcgaacgca atagtgacaa atcatgcacc cgcagaactt gtccctcatt tttcgggctt
    16141 ttttgatcgg atcgtggttg atgctccttg ttcaggagaa ggaatgttcc gaaaagatcc
    16201 aaatgcaata aaagagtgga cagaagaatc tccgctatac tgtcaaaaac gtcagcaaga
    16261 gattttgtct tcagcaataa aaatgctaaa aaataaagga caactgattt attcgacttg
    16321 tacgtttgca ccagaagaaa atgaagagat cattagctgg cttgtagaaa attatccagt
    16381 aacgattgag gagattcctt tgactcaatc cgttagttca ggacgttctg aatggggaag
    16441 cgtagctggt ttggaaaaaa caatacgtat atggcctcat aaagatcagg gtgaaggaca
    16501 ttttgttgca aagctgactt ttcatggaca aaatcagatg cataaagaaa agaagacacg
    16561 aaaaaaaagt aaagtgcaaa tgacaaaaga gcaggaaaaa ctctggacgg agttttcaaa
    16621 tgattttcat tatgaagcta ctggaagact tttagtgttc aacgaccatc tttgggaagt
    16681 tcctgagtta gccccttctc tcgatggttt aaaagtcgta cgtacaggtt tgcatttagg
    16741 agatttcaaa aagaatcgat ttgaacctag ctatgcatta gcgcttgcta caaaaaagat
    16801 agagaatata ccttgcttgc cgatcactca aaaggaatgg caatcttaca cagcaggaga
    16861 aacatttcaa cgtgatggga atcaaggatg ggttttgcta gtattagata agatacctgt
    16921 cggatttggg aaacaagtaa aaggaacagt aaagaacttt ttccctaaag gattgagatt
    16981 tcactgagtg gattagttcg tttatttttt tgtctatgct atgatggaga tagataaaaa
    17041 ggagctgata cttatgaaaa catcaacaaa agtaacaatc ggattaagcg tagcagccgc
    17101 tgccagtgta gcgacagcag tcgttgtatc gggaaaagta atggaaaaga tccatcatat
    17161 gactactcgg acgaaagtaa aaaaattcgt ctatgacaaa tttgatggaa atgaaaaatt
    17221 actagaaatc gtagatggtc tttcagatag tgacttggat tctttgatgg gaatgttagc
    17281 aaaaatcaaa tcaggaaaaa agaaaatctc tgtttatggt gattcaatca aagattcaac
    17341 cgaagatgta aaagaccggc taatgcattt tgttgaaaaa atgatgtaag agaaactagc
    17401 ttttgaacac tattcattcg aaataagggg ttgtgacaaa attattgtca caaccccttt
    17461 aatctatctt taattatgtt catgaagcaa taaaagatag caaaacagta aatcgttggc
    17521 tttttttaga ttgaaaccag actgttcttg aaatttttcg attcgatatt ttagagtatt
    17581 acgatgaaga aacagtgttt ttgcagcaga gctgacattg cctaattcat tccataattc
    17641 ttgaatgatc acttccattt cattttgttt gatcagttct atgtaacttt tgatcaactt
    17701 actatctttt acagtatctt ttgtatagta atgtaaagcc acatcttgca tagaaaaagt
    17761 acggctgtga gtataaagat tgttttgctc tgttaaaaaa atttttcttt cttcagcaaa
    17821 caaagggacg aggtcggtgc caggtgaatg gaaagaacca acgaatacag acgttgtcgt
    17881 atcaaaatca gcatctaaag atagaaaaat accgttgatt tcagtagcat caagataatc
    17941 acggtttatt tgttcgacta agagcgcatc tgtgtctgta taaaaaaaga aatcagcaga
    18001 atagcgaaat aattccaaaa tagtatcttg ccattctttt tttaagaatt ctccttttga
    18061 ttcgacgtga agctggatca aacgaaagct tttttcttga acacaagaag catctttaaa
    18121 aagatagtga taccatgggt gttttttttg atcgttgatt acagggaata acgctttcaa
    18181 aaggctggtt tcttgttgag ataatgaatc ttgtttgatc cagataaaag catcatcaat
    18241 tgcaaaagaa aggatatccg cttctgatgc tggagagttt tttttctgtg cttgtgggta
    18301 aagagcaagt aatttatcca ttttcatctt gttcacctct tgtatttatt ttaccataga
    18361 aaaaaagtta gttacagcct ttttatcata tgctaaagta aaacaagaaa aaacaaaggc
    18421 agggaagggt aattggtaaa tacaaatctt gatgcagtag aaaaagaaaa atggatgcgc
    18481 cttgcattgg cagaagcaaa gaaagcagaa atgcttcatg aagttccgat tggagcagtg
    18541 gttgtattgg atggaaaagt gattggaaga ggatataacc ttagagaaac cacgcaagat
    18601 gctactacac atgcagaaat gcttgcaatc aaggaagcct gtgaaaaagt gggaagctgg
    18661 cgcttggaag atgctgcact ttttgttacc ttagagcctt gtcctatgtg cagcggcgcg
    18721 atgattttgt ctcgtgtagc agaagtttat ttcggcgcgt atgatccaaa aggcggaaca
    18781 gccggtacct tgatgaatct tttggaggac gagcggttta accaccaagc atatgtagaa
    18841 ggtggcattt tagaagacga atgtgggatg attttgactg actttttccg aaagctccgc
    18901 cagcgaaaaa agaaattaaa aaaagactgg tcaacgaatg aaaaataagg tatactattc
    18961 tttgccgaaa ggctaggaca atggtgggct tgtgaagcgt gtcaggtctg gaaggaagca
    19021 gcactaagca ggttccgcca tgtgtctgat tataatgaaa cgacaacaga ctttcgggtc
    19081 tgtttttttt atttttatag tactgtaaaa atgaatgttc ctgatttttt agccagactg
    19141 atttatcgaa tattgtactt atacaatttg cttcaaggca ggaaaaactc tgaaatagag
    19201 cttgtttcaa tggaagaaaa agctgcctat ggtatgataa agataaagag aggcgagagg
    19261 gtacatggaa aaaatagtga ttttacatac gaatgatctg cattctcatt tagaaaattg
    19321 gccaaggatc agaaggtttt tggatcaacg taaacgagaa aacgaaaaga aagaaaaaac
    19381 gacaacgatt gcagtagatc taggtgattt tgtggatcgt tggcatccat tgtcagaagc
    19441 aacgaatgga caggaaaata tcttgctgat gaatgaagtt ggttatgatg cagcgacgat
    19501 aggaaacaat gaaggcgtag gaaattccaa agaagagttg aatcatttat acgatcatgc
    19561 tgagtttgat atcatcctgg ataacttatt cgataaaaat acgctccagc caccggcatg
    19621 gacgaaacca tataaaatca ttacaacgaa acaccagacg aaaatcggac taattgcatt
    19681 tactgctcca tttccgctta cttataatcc gaatggctgg gacatacgga atccttatga
    19741 tattttgcca gaattagtcg aagatttacg ttcacaagtg gatgttttag ttttgatgag
    19801 tcatctcggg attcaggatg atttccaaat tgcaaaagaa ataccagcaa ttgatgttat
    19861 tttaggatcg cacacccatc atttattcag agaaggtcac gtaatcaatg atgtccaaat
    19921 agcagccgct ggaaagtacg ggcagtatgt cggtgaagtc catttaacca tcgatgaaga
    19981 gaaaaaaatc cttaagcatt cagccaaagc catccctacc gaaacaatga cagcttttac
    20041 agaagatgaa caagaaataa atagttgtct agaacgtggg catgaattat taaaagaaaa
    20101 agaagtagca gatttgccat tttctctgag tctagatatc ttcaatgagc attcgtttat
    20161 ccaagtagcc tttgaggcgg tgaaagaacg aggaaagacg gaagcagcaa tattaaacag
    20221 tggtctgttt ttaactgaaa ttccaaaagg gcgggtcaat caggatcagc ttcacactgc
    20281 gttgccgcat ccgatgcact tgttaaatgt cacattgggt ggaaatgatc tgatccgctt
    20341 agttttagaa atagaaaaaa atcgtaactt tttacgtaac tatcctatga agggaatggg
    20401 atttcgcggt aagatttttg ggcagatggt ctataatgga atcacatatg attctattaa
    20461 ccatcaagtc ttttggaaga acaagccaat cgatctgaat aaaaattata cttttactac
    20521 cgtagatcat ttgatgtttg ttccattttt tccaacgatt gagattgctg gaaaaaatga
    20581 atttttgttc cctgaattta tccgaagtgt ggtaggagat tacttgaaag ctcactaccc
    20641 aatcaaataa aaacaaggta taataaaaaa gataggtggt gaacgcatgt ctgcaaaaca
    20701 aaatgaacag cagaacgttg agcataaaac agaaattgct aagaaaaaat catcagctga
    20761 acaggaaaca acagtcaccg aagaactgac agctgctttg gaagaaatcg ttgaggagcc
    20821 taaagcagaa aaagtcaaag gcgagctagt gacactgtta gatgacagta attttttgat
    20881 aggtgaaaga cattatcggc ttgtgtccga ttatcgtgaa ggatttaatg cagaaaagct
    20941 tggagaacgt tacagtgatg tactagcccg atatgattat atcgtaggag attggggtta
    21001 tgaacaacta cgcctaaaag gatttttcca agcggataat cgacgggcgc atccagatca
    21061 gcgaatcgat tccttggaag actatcttta cgaatattgc aattttggtt gtgcgtattt
    21121 tgtcatcgag agaatcggcg gtaaaaaaga aaagacaact cagcgccgaa aaaagaaaaa
    21181 aaaccataat cgtgcgtata tcgatgagaa aaaaggacca gtcgcatcta atcgcaaaaa
    21241 accggttatt aaaaatcgaa aaacagagtc agcaaaaacc tctgaaaaag taaaaaaaca
    21301 agaacaacct aaacaagtag aaaaaacaaa aacagcattt acgatcagaa aaagggaaga
    21361 gtagtggatg aatggactat aaaggatatt taattgattt agacggaacg atttatcgtg
    21421 ggacacaacc cataccggcc ggtaaacgat ttgtggaaga attgcaaaaa agaaaattac
    21481 ctttcttgtt tgttaccaat aacacgacta agtcaccaga aacagtagct aatcgtctag
    21541 cagatgagtt tgacattcat gtagctccag agacagtcta cacagctact ttggcaacaa
    21601 tcgattttat gaaagcagat ggaaaaggga aaaaagtcta tgttatcgga gaagcaggat
    21661 tgattgactt gattttagct gccggattta catgggaaga agaggctcct gactatgtag
    21721 tggtaggctt agataattat ctgacctatg aaaaagtagt taaagcaaca cttgctattc
    21781 aaaaaggtgc tactttcatt ggaacgaacc cagataagaa tatacctact gaacgcggcc
    21841 ttcttcctgg agcagggtca gtcatctctt ttgtagagac agcaacccaa acgccgccga
    21901 tctacatcgg aaaaccagaa gccatcatta tggataaagc cgttgaagta ttagggctgc
    21961 aaaaagaaga agtaatcatg gttggagata actatgaaac agatatccaa gctgggatac
    22021 gcaataatat agatactttg ctcgttttat caggttttac aaaagaagaa gatgtacctg
    22081 ggttgcctat cccagctacc tatgtgaaac aatcactgga tgaatggagc ttctaatatg
    22141 aaaaattcga gatggcagtg gatggaatat gctggattgt tcagcctatt tctgacactg
    22201 atcagtctgg cagttggtgt gacgatcaac tttcgaccac tatacgtatt tgatatcgga
    22261 catttgcaaa ttttggatta tacttcttta gatcaagaga cgttactgaa aaattttgat
    22321 cacctaatga attatctcaa taatcccttc aaaaccatcc tcagcttgcc agatttccca
    22381 gtttctgcta gcggagccca tcacttttat gaagtgaaaa ttttgttttt agtggattat
    22441 gctgtgtttt tcatcacttt gatcccgagt attttgttta tcaaatattt gcaaaagaat
    22501 gatcggcttt ggcggttgat tcgtccgttt caaatcggca tgttactgcc agttgttttc
    22561 ggttttttca tgatgatagg atttgatcgc ttttttatcc tttttcatga aacgtttttc
    22621 aacaacgatg actggttatt cgatcccgtt actgatccaa tcatcaatgt acttccagaa
    22681 gaatttttca tgcatagctt tatcttattt tttgtattat tagaattatt ttttgctgtt
    22741 tttcttttct taggaaaaaa tagcttgaaa caaacaaaaa agaaagaact cgtctaaata
    22801 gacgttttct ttcttttttt gttgaattat tgagtaaggc gacttctttt ttctggttct
    22861 ttgtgtgcta atcgactggc agcagcagcg gcgatcgctc cgacgatatc atctaagaag
    22921 gtatggacat gaacgccatc gtggctgttt agttctttta aaataccagg tttcaccttg
    22981 tcgatataac cataattcgt aaagccgatc gtaccgtaaa cattcacgat tgacaaggct
    23041 aggatctcat cgataccgta aaggccttcg tcatcttcga taatatgttg caacggactc
    23101 atcaattttt tcttttctgc taatatatct aattggattc cagtgataat ggcgttatgt
    23161 acttcacgct ttgttagcac gctgttgaca ctttcagtac aatccgccaa agtcaaatca
    23221 gaaatatagt ctttttgtaa aaacatgaca agttgtgcga tatcttctac agttacgcca
    23281 cgttccttca atagttctct tgccttttct tctaaagtct cagttttgac gaccatatta
    23341 ttgctccccc tttatttttt ctttctctat ttcatgttaa cggaacatga aaaaaatgca
    23401 agaaactgct ttcgggttta acttgaaatg caaagagata taaaaaatca actaggacga
    23461 agatttttat cgtataagct tgtgctgtga ccaggctgag ttcgttcctt tggattaata
    23521 tagtgtaaag cgttgttcac tgcagtaggt gcttcaccga gcccagtagc aatcagtttc
    23581 acttttcctt cataggtaca gatgtcacca gctgcataaa cgcctggaat agaagtagac
    23641 atatctgatt ttacagtaat ggcatttctg gtactatcaa gtccccaaga agaaagatgt
    23701 tctaaattag aagagaatcc ataattgacg atcagagaat caatcatcaa atcaatcgtc
    23761 tcgtcacttt tcactttttt taaccggata tctgtcagtt ccccattatt gcctgataat
    23821 ccatcgatga gatagggagt aagcaagttg acagatgaag atttcaagcg tgatacacta
    23881 tgttcgtgcg cacgaaattc cgggcgacga tgaatcaagt aaacttcgct tgcaataggc
    23941 tctaacatca acgtccaatc gatagcagaa tcaccacctc cagcaatagc gacttttttt
    24001 ccagcatact tcatcaaatc attcacgaaa taatccaatc cgtggttttc aaatgattca
    24061 gcattatcta aattcaattt tcttggctgg aatgaaccat ttcctaaagc taaaatgact
    24121 gctttggaat aatggatgcc tttatttgta gtaatctcga taacttcgtc ttcacgagta
    24181 agcgagagaa cttcttcttt taaatggaat gtatggttaa aagtagttaa ttgtttttct
    24241 aattgatcga ttaattcact tgcttttatt gctggatacc caggaatatc gtaaatatat
    24301 ttttcgggat acaaagttgc taattgacca ccaagttgag gtagactgtc aatgattttt
    24361 gttttagctt ggcgcattcc tgcgtaaaaa gcggcaaaca tccctacagg acctgcaccg
    24421 atgattgtaa tgtcatagat ttccataaaa attcctccct tttcacacta tatcagaatt
    24481 taattgagaa tgcttctcaa aaatgtttga attcgaaatc gaaacggtaa gttatagaat
    24541 gtatctagtg atgaaaaata aaagaaaaca aatctagtat ttttacgaga ggttaggtat
    24601 tatgttatta ttattatgaa gtatgcaaat attaggagga aacaatatgg cattcccaca
    24661 actagattta aaaagtgaaa aaggaccaaa agctgttatc aaaacaaacc gtggtgacat
    24721 tactgttcaa ttattccctg aactagcacc gaagacggtt caaaatttca ttgaactttc
    24781 caaaaaaggt tattatgatg gcgtgatttt tcaccgcgtt attcctgatt tcatgatcca
    24841 aggcggcgat ccaactggaa caggaatggg tggcgaaagt atttatggcg aaagttttga
    24901 agatgaattc agtcgtgaac tgttcaacct tcgcggagct ttatctatgg caaattcagg
    24961 tccaaacaca aacggaagcc aattctttat tgtaaataac acaaatgtgc cagctaatat
    25021 gcttggacaa ctagaaggtg ctggattccc atcagaaatc attgaagcgt ataaaggtgg
    25081 cggaactcct tggctagatt tccgacatac agtctttgga catgtactag aagggatgga
    25141 cacagtagat gaaatcgcaa atgtacaacg tggtccacaa gaccgacctg tacatgatgt
    25201 agtgattgaa acaattgaaa tttcagaata gtttggtatg gtacggagta attatgaaaa
    25261 aggacgaaac ctagtaaggt ctcgtccttt tattttgaat aaatgattta atcgattgtc
    25321 tctaaagcat acttgattcg attaagtcct tccagtaaag tttcttctgg acaagcaata
    25381 ttcaagcgca tatgcccatg accggaagga ccaaacgtga tgccaggatt taatacgact
    25441 tttcctttcc gaacaagagt gttctccaat tcttggtcgt ctgaaatata ggcggtaaaa
    25501 tctaaccaca ttaagtaagt tccttcaggg ttcattattc tcactttcgg aagatactgt
    25561 tcaaaaaact gattaactgt ttgaacattt ttttctagat aaggaatcag ttggcttaac
    25621 cattctccac ccgtctcata agccgcttgg gttccaatca gtccaaaagt gttgatttcg
    25681 tgctgttggt tcatcactaa atgtttttca aaatcagctt tcatctctgg atttttgata
    25741 aataccattg aatttttgat tgcagctaga ttgaaagtct tggttgcgga agtaaaagca
    25801 actagtagat catctaaatt aggatcaatc gttagcatgg aagtaaatgt atgatcgaat
    25861 aaagttaaat cttgatggat ctcgtcacta aacagaatca cttgatgttt taaacacaat
    25921 tcgcttaact gtttcaattc ttctttgctc caaacacgcc ctccaggatt gtgaggattg
    25981 cataaaatca ttgcttttac attgttttct tcgatttttt tctccatgtc agacaagtcc
    26041 atgatgaaat gattattttt ttctagcaaa gagcttcgaa cgatgtgacg ttgatttgtt
    26101 tctataatag aggaaaatgg aggataaact gggtcatgaa tcaaaataga atcgcctggt
    26161 tttgtaaaag tttgtactgc tgcagctaaa ctggttaaca caccacttgt aaaaacgata
    26221 ttttctttcg tcaactccac agcatggtgt tggcgttccc aatgaatgat agcctgatat
    26281 agtttatcag acagagtggc ataaccaaag attccatgtt tgatgtattc actaaacgct
    26341 ttagctaccc caccgggaga caagaagtcc atatctgcta cccacaaagg aagtaagtca
    26401 tccatttgat atgtctcagc aattgcgtcc cattttacac tttctgtatc ttttcttgaa
    26461 atctgtttat caaattcgat catcttaaga cctccagtca ttttcgtcgc ttcttttttt
    26521 gagctattta cagtataatg ataaatgcga agagagggaa gtagatgact tataaaattg
    26581 gacaaataat tgaaggaaaa gtgacaggga tacagcctta tggcgcattc gtttctcttg
    26641 ataacgagac gcaaggactt attcatgttt cagaagtaca atccggttat acgaaaaata
    26701 tccattcatt gttaaaagtc ggacagccag taacagttca gatcatcgac attgatgaat
    26761 attccaaaaa aatcagttta tctttaagaa cgttagaaaa aacaaatcca gctgttccat
    26821 atcgcagaaa aaggtatttc acgaataaaa atcgtaagat cggatttacc actattgcgg
    26881 agcagttacc tatttggata gaagaagcat tatctgaact agaacggaaa gaaaataaga
    26941 aataaaaagt gtattttttc tgaaaatgtg ctagaatttt tatgtaagcc atagaacaag
    27001 ttgatcgagg tggaaatttt ggcaagcaaa agagttgctg gtacaattat gttgcatttg
    27061 gaggatggta ctaaaaaatt tttggtccat tcaattgatg acaagttgga atttgcgtta
    27121 gcggaacttt ctgaaggaaa gactggttta gcgaatatac ttaatttctt aaaagaaata
    27181 gttcatatcg atgttagcaa gattagtttg atggaattga caaatacaca tattaaagag
    27241 gaaaatgttc cgttgtttgt ctttgaaact gaacaaaaga atcaaagaga agaattaggc
    27301 gaaggatata tttgggaaga accaggtcaa atgcgctctg ttcttggaac ttacgaagta
    27361 gagggagtgc ctttcttcta aaatacagat taagcgaaca aagaagtttg aatttttttc
    27421 aaacttcttt ttttattgtt tggtccaaaa aatacaatga aatgttctgc taaagatagt
    27481 tttttagtgc attttatttt caaaatatgt tgaaaacggt tgtataacga ataataataa
    27541 ttgatcgaaa aatcatttca caaagtaatg cttaatttat agttgaccaa ggaagaaata
    27601 catgataaat ttaacagcgt tgattctgaa gtggatgtta tatcacattt ctaaaaa
//
LOCUS       DAZAFF010000031        27292 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000031 DAZAFF010000000
VERSION     DAZAFF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.031"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..1470
                     /locus_tag="ACG9BB_001269"
     CDS             301..1470
                     /locus_tag="ACG9BB_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN2837222.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            1943..2497
                     /locus_tag="ACG9BB_001270"
     CDS             1943..2497
                     /locus_tag="ACG9BB_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2837223.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(2670..3143)
                     /locus_tag="ACG9BB_001271"
     CDS             complement(2670..3143)
                     /locus_tag="ACG9BB_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2837224.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKIKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            3155..4102
                     /locus_tag="ACG9BB_001272"
                     /pseudo
     CDS             3155..4102
                     /locus_tag="ACG9BB_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            4373..4808
                     /locus_tag="ACG9BB_001273"
                     /pseudo
     CDS             4373..4808
                     /locus_tag="ACG9BB_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            4824..5657
                     /locus_tag="ACG9BB_001274"
     CDS             4824..5657
                     /locus_tag="ACG9BB_001274"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2837225.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            5902..6927
                     /locus_tag="ACG9BB_001275"
     CDS             5902..6927
                     /locus_tag="ACG9BB_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2837226.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            7065..7190
                     /locus_tag="ACG9BB_001276"
     CDS             7065..7190
                     /locus_tag="ACG9BB_001276"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837227.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            7215..8330
                     /locus_tag="ACG9BB_001277"
                     /pseudo
     CDS             7215..8330
                     /locus_tag="ACG9BB_001277"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            8413..9309
                     /locus_tag="ACG9BB_001278"
                     /pseudo
     CDS             8413..9309
                     /locus_tag="ACG9BB_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            9325..10149
                     /locus_tag="ACG9BB_001279"
     CDS             9325..10149
                     /locus_tag="ACG9BB_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2837228.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            10181..11491
                     /locus_tag="ACG9BB_001280"
     CDS             10181..11491
                     /locus_tag="ACG9BB_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFN2837229.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            11639..12898
                     /locus_tag="ACG9BB_001281"
     CDS             11639..12898
                     /locus_tag="ACG9BB_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN2837230.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            12908..13780
                     /locus_tag="ACG9BB_001282"
     CDS             12908..13780
                     /locus_tag="ACG9BB_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2837231.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            13792..14622
                     /locus_tag="ACG9BB_001283"
     CDS             13792..14622
                     /locus_tag="ACG9BB_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2837232.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            14662..16845
                     /locus_tag="ACG9BB_001284"
     CDS             14662..16845
                     /locus_tag="ACG9BB_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN2837233.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            16906..18126
                     /locus_tag="ACG9BB_001285"
     CDS             16906..18126
                     /locus_tag="ACG9BB_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFN2837234.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            18123..19583
                     /gene="gtfA"
                     /locus_tag="ACG9BB_001286"
     CDS             18123..19583
                     /gene="gtfA"
                     /locus_tag="ACG9BB_001286"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFN2837235.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            19660..20766
                     /locus_tag="ACG9BB_001287"
                     /pseudo
     CDS             19660..20766
                     /locus_tag="ACG9BB_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            21088..21297
                     /locus_tag="ACG9BB_001288"
     CDS             21088..21297
                     /locus_tag="ACG9BB_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN2837236.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            21290..21439
                     /locus_tag="ACG9BB_001289"
     CDS             21290..21439
                     /locus_tag="ACG9BB_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFN2837237.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            21593..22258
                     /locus_tag="ACG9BB_001290"
     CDS             21593..22258
                     /locus_tag="ACG9BB_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2837238.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(22542..23627)
                     /gene="xerS"
                     /locus_tag="ACG9BB_001291"
     CDS             complement(22542..23627)
                     /gene="xerS"
                     /locus_tag="ACG9BB_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFN2837239.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            23961..24194
                     /locus_tag="ACG9BB_001292"
     CDS             23961..24194
                     /locus_tag="ACG9BB_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFN2837240.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            24191..24598
                     /locus_tag="ACG9BB_001293"
     CDS             24191..24598
                     /locus_tag="ACG9BB_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFN2837241.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(25735..26253)
                     /locus_tag="ACG9BB_001294"
     CDS             complement(25735..26253)
                     /locus_tag="ACG9BB_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFN2837242.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            26620..26868
                     /locus_tag="ACG9BB_001295"
     CDS             26620..26868
                     /locus_tag="ACG9BB_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837243.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         9338 a         4142 c         5036 g         8776 t
ORIGIN      
        1 tatataaatt aataatatac taccatttgt tggtagtata atttaaaggt aataagttat
       61 tcttttatta ctatgtttgg gttttatagc aaagaaagtt taataagagt ttggacctag
      121 caaacaatat taatgaggtc attaaatctt attatcttta gtgatataac gaaatatcta
      181 cacagacaaa atgatagcgt aaaatatttt gtgtaaatag atatttaggg tagaaaaaaa
      241 gaaagtccat tctgtaaaat taaagttacc acaaccgaaa ttttatagga ggactttctc
      301 atgcccaatt ttactacaga aattatggaa acactaatca ataaaggtga tttagatgaa
      361 ttatttcgat gtcatttaga attagctgtt aattcattat tgcaggctga attaacagct
      421 tttcttgatt atgaaaaata tgatcgtgct gggttcaatt caggtaattc ccgtaatggg
      481 aattattcac gttcattcag aacagagtat ggagaattaa atttggtaat tcctagagat
      541 agaaatggta agttttctca acaaacttta cctgcttata aaagaaccaa tgactcttta
      601 gaaaccacta ttattcagct atttcaaaaa ggaattacta tgtctgaaat ctctgaattg
      661 attgaaaaaa tgtacgggca tcactatacg ccacaaacca tttcaaatat gactaaaata
      721 gtttctgaag atattattgc ctttaaagaa aggtccttag aatccagata ctctgtcatt
      781 tttatggatg ctactcacat tcctttaaaa agacaaactg tttcaaaaga agcagtttat
      841 attgtaattg gcattcgatt agatggaacc aaagaggttc ttgggtttag tcttgctcca
      901 actgaatctg cttatgtttg gaaagagatt cttcaagacc taaaagaccg tggtttaaaa
      961 gaggttttat tagtcgtaac tgatggttta agtggcatta acgatagtat tcatagtgtt
     1021 tatccaaatg ctcaatttca acaatgttgt gtccatatat ctagaaatat cgctcataag
     1081 gttcgtgtta gtgatcgaca agaaatctgt agcgatttca aattagttta tcaagcaagt
     1141 tcaaaagaag aagcaaataa tcaaataagg tttatgatag ataaatggaa aaagcagtat
     1201 ccacgagtgg ttaaattgct catgaatcct gctatattaa ccttttataa tttccctccg
     1261 tcaatcagaa gaactattta ttcaactaat ttgattgaag gttttaacaa acaattaaag
     1321 aaatatacca aaagaaaaga gcaatttcct aatgaagaat ctctggagag atttctagta
     1381 tctcaattca acaactataa tcaaaagttt ctatgccgag ttcataaagg ttttaaagaa
     1441 atacatgata cattagaatc aatgttttaa cttgatcaac agaatgggct tttccattta
     1501 cacaaaatta ttgacgctcc cgacaaaatt atgatcttgc gtaaagttat aaaatattaa
     1561 taaaaatatt ttattaatga aaagcttggt agcacggtta ttcatatgct aatttatttc
     1621 actttatagt aaggggagtt cttagtatat tgttcaaact taaatttcat ataatttaat
     1681 tatgtaaatt agctattcta agaacttcta cggataactg cccgtctaaa atttaaataa
     1741 gagcttagaa tcttcatata agtaaggtta atttttttgt acaaaaaagt cggaatattc
     1801 tgagttactg aaatgatata aaatcataaa ctaaatccct tgattttatt gactttcaaa
     1861 agtttatata tataagttaa tgattatggt aaaatatata cataatcatt aactcagaat
     1921 atttttaaga agggaaacaa tagtgagcta taatgtacaa ccgttaagga caaatgaaga
     1981 gatacaagat tttcagtttt ggctaagaag aacaagtaat ccggaaagag atagtttttt
     2041 atttttgttt ggaatcaata atgggttacg tatgtcagat attataggat taaaagttgg
     2101 agatatacga ggcaaaagta agcctattat tgttgaacat aaaacaggta agcgaaaacc
     2161 gatttttata gataatttga gagaagaaat tttattatat actgaaggta aagaagaaaa
     2221 tgattggtta tttccgagtc ggcaacaagg tcgacatata acaagggatc gtgtctatca
     2281 gatatatgca gatatagcag aaaaattagg acgagatgat ataggaacac atacattaag
     2341 aaaaactttt gggtatcact actacaagaa aactagagac attgctacct taatgtttat
     2401 ttttaatcat agctcgcaag caattacaaa aaggtacatt ggtataactg aagatgaaat
     2461 aggagcatca cttcgtggat ttaaactagg tgtataattt aaaaattttt ataaacagta
     2521 aagacaaaga ttaaaagcta tttttatcta gaacgatttt atttcataaa gttcgcggaa
     2581 taaaagctgg ggttactggt acttggtata ggttcgtgtt atataaacta agtaattaaa
     2641 taacattttt aggttggaaa aggatgaaat taaaaaaaga gaatcaatac ggtctcaaaa
     2701 acgcaaacgc tgcggaataa gaaaaatact gataactcaa cgtttagcag actatctaat
     2761 agggatagtc tgcttttttc gtttttgacc atacttttga tcttctgcaa acttccttgc
     2821 ttttcctctg tagaactcat gaaatctgca aaacgaattg ccgtttcctc tgtctgcttt
     2881 ttcgcgatat gggtgtatat atcatccata gtggttttaa tatctgtatg tccaagtcga
     2941 acatacactt ctttgacaga cgcaccagat tcaaagagca gactacaatg ggtatgacga
     3001 aaaccatgaa gtgtgatatg ttttaagtta ttctctttaa tgatgatttt caaattgtga
     3061 tttacatagt ctaaataaag gggcttattt tcttctgatg aaaacaaaag ttgttcacca
     3121 tcttttatag gtttgattct catttttaga tattatgccc gcacgtattc atcacatttc
     3181 tattgtgaat cgctttattc gaccgacatt tgacttttat cataatatac tcggtttaaa
     3241 attactaatg aaaacaatta atcaagacga tcataccatg tatcacctgt ttttttcaga
     3301 taatcaccac cgtgtgggaa ctgaactgac tttttttgaa gtacaagaag ggaacaacca
     3361 gttttttgga accaatacga ttgaacgaac gatactaaaa gttccttccg aagcatcatt
     3421 gcatttttgg gaaatatatt ttgaaacaca aggcgtttgc cattatggaa tcgaatcatt
     3481 ttctggacga cctatcttac gttttgaagg gccagatgca acacgattag ctttagttcc
     3541 cttacgcgaa tttgaagata ttgatgatta tttcccgtat gtcacagacc agatccctac
     3601 agagcatgcc attttaggaa ttgatgccat tcaactacgc gtacaatatt ccgatgcgac
     3661 tgaacgagag ttattgtcgg taggctggta gcacaaagaa tacgtcagtt tcttttcggg
     3721 tcatcaacga gtgacaatct tagaaaatca agacactcag ttctatcaag aagttcagat
     3781 tattcaagat cgtgagaatc cagttgccat tgaagggatt ggtagcgtcc atcatgttgc
     3841 gtttggcgta gaaaataaat ctgatttaca acgcatcgac aaacaattac aagagcgcaa
     3901 ttttataaat tcaggaataa aagatcgtga attttttgta tcactttatt atcgtgaccc
     3961 aaatcaatta ttgattgaaa ttgctacagg ggaaggaaac ctagatgcaa aagcgtatga
     4021 aaatcaaagt ccacgctttg aagaaattcc tttattttta ccgcagtact taaatttcat
     4081 tcgtgaacaa gtggagcaat aattagctaa tcaaatccat gacgaagagt aagaggttga
     4141 ctcataagtc aaaaaataaa gaaaaacgca tcaaatccat tctaaagaat gttgatttga
     4201 tgcggttcct atttttataa actatcattt tcgactaata aaactactta tgagtcagcc
     4261 tctctttttt tcttgatttt tatacctctt tctaggtaaa ctaaacatct gccaaaaata
     4321 ccgtgtctca ttgaaacatt ctcaactatt tcaaaataaa ggggagtacc aaatgaacga
     4381 gtatattgaa attatccacg caagagaaac aacttaaaaa tgtcagtctc aaaattccca
     4441 aaaataaaat tagtattttt accagcgtat ctggttcagg aaaatcatcg attgtttttg
     4501 atactattac ccaagaagct ggtcgtcaac tgaatgacac ctatgatagt tttacccgtt
     4561 tatttttacc taaatacagt cgtccagagg ttgatgagat tgataatctt tctacggcga
     4621 ttgtggtcaa tcaaaagaag attggtggca gtgcgcgctc aacgttaggg acgattacta
     4681 acatcaatgc gttatttcgt gtgttgtttt catgttttgc aacacctagt ttaggatatg
     4741 caaatgctta ttcatttaat gatcctagtg gaatgtgttc tgtctgtcag ggaatcggta
     4801 agacgattgc atttttaata ggattgcttt atttttcggc tattgataat gctgattttg
     4861 tattttctga tccatttacc gcagaagttt tgtctggaat tgaggaacag gcaaagaatc
     4921 atggttattt tgtattggtg cataatgtta attcagcaga agatgttaaa actattcaga
     4981 gaaattggcg ttttgagggg ttcattgttg ttggtgtacg tgtaaaagaa tttttggaat
     5041 taaataacgt ttttgatggt cctgttgttt atattgatac gcatctttca aaagagacat
     5101 taagagaact tgaacaacaa atgaatcgtt tatttgtaaa tacaaatgat taccaagcta
     5161 gtacattggc agtggaacat ttgattcatt atggtcatga gaaaatagcg tttctttctt
     5221 ttgactacat tgtaggtgaa ccaagtgtta ttcaagaacg acatcaagct tatatcgata
     5281 cattaaagaa atatcattta acctttaaat cgcattggat atataacaac tctgaatttg
     5341 agcgaattta tcaagaatta gctgaattta ctgcaattgt tgttactgga gattatttag
     5401 cggctaaatt agtaaaattt ttaaaaaata agaataaacg agtgatggaa caattatcga
     5461 ttattggatt tgatgatatt acttttgcag aattaatgga tcctgcgttg acaacaatac
     5521 gactaaatcc aggtaacaag ggggcgattg cttttcacca actgattgga ctggccaaac
     5581 aagaagaaat aaaaaatcaa gtactactat tagagggaga attgatttct cgagactctg
     5641 ttcacaaaat caaataaaaa agctctgtga aataagtata gttaacaata aaatatccga
     5701 acgatagaga gaatgaactg tgatctagca gttcctcgtc gcaaattcac tagaattagc
     5761 attatttact ctaaattgtt cggatatttt ttcttgtttt taattatgat tacagtgcct
     5821 atttttaacg aaatttgctt attttcaaaa tgtatgatta tatgttagta aacactttca
     5881 aatatgaaaa gaggagctat tatggtagga attagggatg ttgcaaaacg ggcgggtgtg
     5941 tccattgcaa cagtatcgcg cgtgctaaat gaggatccga cactttcagt aaccgatgaa
     6001 accaaacgaa agattgcaga atcggttaat ttttatcatt atgtcaaaaa aagtcccgtt
     6061 gcaaaaaatt taacaaatat tgcattaata acgacagttt ctgaagtaga tgagttggaa
     6121 gatccctatt ttcgaacgat tcgcagaggg attcaagtcg aagcagaaca aagcaaagtc
     6181 aatttgaaga aaattattcg tttgtcagaa acaccgttgg agcaagacga tttgctaaaa
     6241 tgtcaaggtg tgttgattat tgggcaggtg ctatcttctg tgattgaaga agtatcagca
     6301 attaattcga atgtcgtagt gattgatgac cctagtattg aagctgaagt agatgcagtt
     6361 tatactgatt taacaaaagc aacgaatcag catttagatc gattgtataa aaaaggacac
     6421 cgaaacattg cgtttatcgg aggaggacgt gttcgtttag atagccaagg aaataaacac
     6481 gtgtcagatg atgattatcg aaaaatagcc tatgagacgt ggatgaaaca aaaaggatta
     6541 gaaaaatata gtcaaattta tttagaaggc tggaccacac ttgacggaat gcaggcagca
     6601 gatttacttt tgaaaaaaca tgctaaagat aagttgccga ctgctatcat ggtaggaaat
     6661 gatcctattg ctgtgggggt ttatcgttca cttcaaaaaa atggtctaca tattccagaa
     6721 gatatttcga ttgtgagttt tgataatatt gaagttgctg aatttttaac acctgcatta
     6781 aatacggtga atgtgaatac agaagaaatt ggaaaactag cagttcgcat ggttaatgaa
     6841 cgaataaaag aagttagaaa tgtttcgatc aaagtgattg tttctaataa tattattgtt
     6901 agagaaagtg aaaaaaatat cgattaaaaa agtaaacatc caaaaaaggg tgtttacttt
     6961 tttgtttact aatattttta gtaaaaaatc tcgacaaaac attaagtaaa cagttatggt
     7021 gttgttaaca aaaagaaaac gcattcaaaa caggaggaat taagatgaaa catttcaaaa
     7081 aaatagcttt aacagggata gctgttttaa ctgggacatt actcgcagca tgtggtgggg
     7141 gagatagtaa ttctaaagac gggcaagtaa ctttaagcta tgcgagttga gatactgacc
     7201 aagctccagg actaagaaaa atgttagatg aatttgaaaa acaaaatcct gatatcaaag
     7261 tagaaatgga gacaacgcct tgggatcaat attgggttaa attagaagct gcaacaacgg
     7321 gaggtaatat gccagatgta gtaaccatgc actctagcga atcctataaa tatatgtcac
     7381 agaatgcttt gatgggactt gatgatgtta tcaaagaaaa tgatattgac atgggaaact
     7441 ttactgaagg aattgctgat ttctatacgc ttgaagacga actatacgca atccctaaag
     7501 atgcttctgt agtggggcta tggtacaaca aagaattatt tgataatgca ggtgtcgatt
     7561 atccagatga aacatggaca tgggaaacac tcagacaagc tgcaattgat ttaacagatg
     7621 aatcaaaagg catctatggt tttgcatcac atattggaac agagactggc tactggccat
     7681 ttatttatca aaatggggga gaagtatttg ccgatgacaa tacaaaatca ggtttgaata
     7741 ccccagaagc acaagaagct ttagcttttt atcgagatat tgcacttgtt gatcatgctt
     7801 caccaacaat tacagaatta caagatcctg ataagttaag tcgttttcaa tcaggtagat
     7861 tagcaatgat gattgaagga aactggtttg caccgggatt tttgaaaaat gactatctag
     7921 ttgaaaatgt tgcttctgcc gtgttaccta agggaaaagt taatgcaaca attacaaatg
     7981 gacttggttg ggctgccagt gcgagtacaa aatacccaga agaagtgaaa aaactaatgg
     8041 catttttagc tagtgaggac gccaataaaa tccaagcgga aacgggggca tcaatcccag
     8101 cactaaaagg ctttgaagac gagtgggctg cttcttcaga tacaattgat ttgagtgctt
     8161 ttggaaaaat gttggagtat ggagtccctc gtccatacaa taaagatgga ttacgagctg
     8221 aacaagcaga agttcaaatt attaaccgta ttctgtctgg tgaagtaact gttgaagatg
     8281 gaaccgctca aattgaaaaa gaagtcaatg cgatattgag cggaaattaa attagttaat
     8341 tatacaatat acaggcgaaa ggattctatt tcgtctgtat attaatggat gaataccaaa
     8401 aagggggata ttatgaaaaa tcgaattttt ggaaaatatc gtaaagatca agtgatatgg
     8461 ggctatattt ttatttttcc aacggcatta ggattaattt tattaaatat atggccagca
     8521 attcaaacag caattttaag ttttcaaaaa acggtagggt tcggtgatac tgaatgggtc
     8581 ggattaagaa attaccaaaa aatgtttgca gacggtgaag tttttcagtc tttattaaac
     8641 acattaattt atgcagtggt ttcgatacct agtattgttg tattatcctt gttggtggca
     8701 gttttaatga atagaaaagt taaaggattg tcgatttatc ggacaattta ttttctacca
     8761 gtcgttgctg caccatccgc agtcgcaatg atttggcgct ggatgttcaa taatgattat
     8821 ggactgatta ataacatgtt ggctaaaata ggtctcgatg gaccagcatg gttaacggat
     8881 ccatcgattg cgatttattc tattatcatt gtaggagttt ggagtgctat tggttataac
     8941 atggtgttat tactagccgg cttataagaa attcctaaag attattatga agcagcggaa
     9001 attgatggtg ctagtccaat ccagcagttt ttcaacatta cgttaccatt agttacacca
     9061 acattatttt ttgtagttgt aacaacagtt atcaatgctt tccaagtatt tgatgttatt
     9121 tttatgatga ttactcctaa tagtaccgcc atgtttaaat cacaatcatt agcctattta
     9181 ttctataaac attcatttat tttaaatgac aagggatatg gttcggcaat cgttatgttc
     9241 cttttagtga ttatattaat tattactgca attcaaatga agttgcagaa aaagtgggta
     9301 aactattaag gaggctatca aattatgaaa aataaatttt tttcaaatgc gccaatccat
     9361 atcatgctta ttttaggtgg tattgcaatg gcattacctt ttatttggat gattttaact
     9421 tcagggaaaa cgttggttga atctacccaa attccaccta caattttccc tgagaaattt
     9481 cagctaagta actatcaaca agtacttgat ttattaccat tcatggcttt ttatatcaat
     9541 acatttttaa tggttgttgg acgagtgatt ggctcggttt tattttcagc aatggctgca
     9601 tatgcttgtg cgcgtctaag ttttccaggg aaaaatatcc ttttcagctt agtacttatt
     9661 caaatgatga ttcccggttc catttttatt actccacagt atttattagc acaaaaattg
     9721 ggtattttaa atacaattcc agcattaata ttaccagggg tagttagtgc gtttggtacg
     9781 tttttattaa gacaattttt cttaggttta cctgatgaat tggaggaagc ggcctattta
     9841 gaaggtgcca atatttggac gatctttttc aaaattatgt tgccgcttgc taaatcagga
     9901 ttggttgcac ttggaatatt cacagcgttg tttgcattta aagatttaat gtggccattg
     9961 attgtcaata cttcaattga taagatgcct ttagctccgg gattagcaaa cttacaagga
    10021 caatatgcca cgaacttccc acaattaatg gcaggttcgt tattagcaat ttggccaata
    10081 gttgtgatat tcatcgtttt ccaaagaaaa ttcattgaag gaattgctac aacaggtagt
    10141 aaattataaa actattaatt agtgattgga gagtaagtac atgacaaaaa ttacatttat
    10201 tggtgcagga agtactattt ttgcaaaaaa cgtattaggc gatgcgatgt taactccaag
    10261 tctacaagat gcacatattg cattgtacga tattgatgaa aaacgactaa aagaatcaaa
    10321 tttaatgctt aaaaatatta ataaaaattc gaatgaaaat cgtgctgata ttgtttctta
    10381 ttatgatcgc aaagaagcgt taagagaggc tgattttgtt atcaatgcta ttcaagtggg
    10441 tggttataaa ccttcaacag tgattgattt tgatattcct aaaaaatatg ggttgcgaca
    10501 aactattggt gacaccatgg ggattggtgg gatttttaga accctacgga cactaccagt
    10561 aatgttagac tttgctaaag aaatggaaga agtttgtcca aatgcttggt tattgaacta
    10621 tacaaatccg atggcgatgt taaccatggg tgttttaaaa gcgacgagta ttaagactgt
    10681 tggtttatgt catagcgttc aagtatgcgt accagaattg tttgagcatt taggaatagc
    10741 cgatcaatat gacttaaatg actttcaatg gaaaattgca ggcattaatc atatggcatt
    10801 cttgttggaa attacaaaaa atggggaaga tttctatcca acaattcgtc agttagctgc
    10861 agaaaaacca aatccacatc cagattctgt acgttttgaa ttgttgaaac gttttggtta
    10921 ctatattaca gaatcaagtg aacacaatgc agaataccat ccatacttta ttaaatcaaa
    10981 atacccagaa ttgattgacg aattagggat tccgttggat gaatacttac gtcgttgtga
    11041 acaacaaatt aatgattggg aaagcatgcg agaggacatt gtaaataatg gggagttaac
    11101 acatacgaga agccgtgaat atgcttcata tatcatggat tctattacga caggaactcc
    11161 gtcagtgatt gcaggaaatg tcttaaataa aggcttaatc acaaacttac cgacagatgt
    11221 ttgtgtggaa gtaccttgtt tagtcgataa aaatggcgtg caaccaacct atgtcggtga
    11281 attgccaaca caattagctg ctttaaatcg gacgaatatc aatgttcaag aactgacagt
    11341 agaagccgct ttaacactag ataaatcaaa aatttatcaa gcagcgtatt tagatccaca
    11401 tctatcatct gaattgtctt taacagatat caaaaattta gtggatgatt taatcgaagc
    11461 acatggggac tatttacctg aatataatta aaaagaagct gaaagagtaa ggaattttca
    11521 attccttact cttttcttag ttaaatttag taaaaatatt gacaaaacac ttagtaaaca
    11581 gttacattag tgttaacaaa aagaaaacgc attcaaattg tataaggagg agcataaaat
    11641 gaagtggtct aaaaaaatta ttttagcttg tatagcacta ggagcaacga tctctttgac
    11701 tgcttgtgga aaaaatcagg aagatgcaag cggtaaggta gaaatagagt tttttaatca
    11761 aaaaaaagaa atgactcaaa cgattcagga gattgctaaa gattttgaag caaaaaaccc
    11821 tgatattcat gtaaaagttg tggatgtacc gaatgctggt gaagtaatta aaacacgtat
    11881 gcttgcaggc gatgtaccag atgtcattaa tctttatcct caaagtatag aattgaaaga
    11941 atgggcgaaa gctggttatt tagaggactt aaccgaagaa ccgtatttag agaatatcaa
    12001 aaatggatat gcacaacgtt ttgctattga ggatcgtgta tatagcatac cattaaccgc
    12061 taatgtctat ggtttttact ataacaaaac tgcttttgag gaaatgggga ttcaagcacc
    12121 agaaacttgg gcagaatttg aaaaaatagt agctgagatt aaagatcaaa acaaagtacc
    12181 gtttgcgatt gcaggagcag aaggctggac tttaaatggt tatcaccaac tagctatagc
    12241 gactgctgct ggtggtgaag aggaagcgaa caatgtttgg cgtttctcag aagttaatgg
    12301 aattaatgct gaaagcaaag aaatgcaaaa agattttaat cgattagatt tgttaagaga
    12361 accaaaagct ttgcaaaaca actggcaagg tgccggttat aacgatacag tagttacttt
    12421 tacaaaaggt gatgcgttga ttatgccgaa tggttcatgg gcaatgccga tgattcattc
    12481 acaaaatcca gattttgaag taggaacttt tccttttcca gcagatgaag caggtcaaag
    12541 tctaacgatt ggcgcaggag acctagcctt atcagtttct gcaacttctc aacataaaga
    12601 agaagccaaa cgttttgtag aatatatgac aacacctgaa gcgatgcaga aatattatga
    12661 tgtagatggt gcaccgtgtg ctgttgaggg cgttatcgag aatactcagg attcaccatt
    12721 aagtggttta actgaacttg cttttactga tcgtcattta gtttggttag ccaaagattg
    12781 gaatagtgaa aatgattttt ataccttaac aacaaattat ttacacaatg gcaatcaaaa
    12841 agccatgatt aacgcattaa atgctttctt taatccaatg aaagctgacg gaaggtagga
    12901 aaataaaatg aaaataaaag aaattttaaa tcgttactgg gcaatcattt ttgtagcaat
    12961 tccaattatt ttgcagatga tttttttcta ctttccaatg gttcaaggtg ctttttacag
    13021 tttaactaat tggactggtc tgacttataa ttttgatttt gtgggtttga acaattataa
    13081 gattcttttt tctgatccga agtttatcaa atcgattggc ttcactttaa ttgtgacgct
    13141 ttgcatgatt gctggacaaa ttggttttgg tattttgatt gcgcgggcgt tgaacgcaaa
    13201 tattaaaggg aaaacattct ttagagcatg gttctttttt ccagcggtct tatcgggcgt
    13261 ggttgtttct ttagtattta aacaaatttt taactatggt ttaccaaaaa ttggtagtat
    13321 gttaggcatt gaatttttac aaaccagtct tttaggaaca acatggggag cgatttttgc
    13381 agtaattttt gttttacttt ggcaaggaat tgccatgcca gtcatcattt tcttagcggg
    13441 ttttcaaagt attccacaag aaattattga agctgctaga atggatgggg cgaatgataa
    13501 gcaaattttt tggaatattg aaatcaccta cttattacca agtatttcaa tggtatttat
    13561 tatggcatta aaaggtggct taacagcatt tgatcaaatt tttgctttaa ctggtggtgg
    13621 accaagcgat gccacaacat ctttggggtt acttgtttat aattatgctt ttaaaaacaa
    13681 tcagtttggt tatgcgaatg ctattgcttt agtcttattt atcttaattg ggattgtttc
    13741 actggcacaa ttaaaattat cgaaaaaatt tgaaatctag gaggtttccc aatgaaaaca
    13801 tcaaaagaaa aaaatatttc taaatacttg cttttattat tagggggctt catgatttta
    13861 gttcccttag tttcaacgct attcggttca tttaaaacaa cacccgatat tatgaataat
    13921 ttctttcgtt ttcctaatcc agcgactttg gaaaatttcc gaagattgtt agcggatgga
    13981 attggtcatt acttttggaa ttcggcaatc atcacaatca ttgcagtagt tgctgtgaca
    14041 gcaattattc ctatggctgc ttattcacta gcaagaaaaa tgactcgtgg gaaagcatac
    14101 ggattgatgt atttattttt aattttagga atttttgttc ctttccaagt aattatgatt
    14161 ccgattaccg ttatgatgag taaaattggt ttagcaaata tgtggggatt aatcttactc
    14221 tatctcactt atgccgtacc acagacacta tttttatatg ttggttttat taaactaaat
    14281 gtgcctatca gtctggatga ggctgctgaa attgatgggg ctagtcactt tacaacttat
    14341 ttcaaaattg ttttcccaat gttaaaacca atgcatgcga cgactattat tcttaatgct
    14401 ctatggtttt ggaatgattt catgctacca ttattaattt taaatcgtga ttctaatatg
    14461 tggacactac cattgtttca atataattat acgggacaat attttaacga ctatggtcca
    14521 agttttgctt cctatacagt aggaattgtc acgattacac tagtttatct tgctttccaa
    14581 agaagtatta tagctggaat gagcaatgga gcagtgaaat aaccaataaa ttattctcat
    14641 tataaaggag ttcataaact tatgattata tttgacaatc acacacaaac atttcattta
    14701 caaaataaaa aaattagcta tctattagga atcgaagaaa ataattattt aacgcatctt
    14761 tattttggaa aaaagattac gcactattct ggtgggtatc gctacccccg aacagatcgt
    14821 tccttctcac ccaacaatgc aaatgcgcaa gatcgtttat tatctttaga cacattacca
    14881 ttagaatttc caggatatgg acatggtgat tttagagaac cggcgcataa tattaaatta
    14941 ggaaacggta gtcgtgtaaa tgattttcgt tatgatagct acgaaattca aaaaggaaag
    15001 cccagcttag cgggattgcc agccttatat acggaagatg atgatgaagc tgaaacgctg
    15061 attattcaat tgaaagacgc gattagtgga ttgactttac agttgttcta cacaatttat
    15121 caaaatcaca gtgtaattat tcgttctacg caattagtaa ataatagtga tgaaacagtg
    15181 gagattaacc gcttagctag tcaatcaatt gattttccaa atcgtccact cgagttaatt
    15241 catttaaatg gtacctgggc aagagaacgt caaatgacta gagaaaaaat tcatacaggg
    15301 ataaaagtat tagatagtaa acgcggatca agcagccatc aacaaaatcc gtttgttgct
    15361 ttagtagatg ccaaaacaga tgaatttcaa ggagaggcat atggatttaa tttagtttat
    15421 agcggtaatc acgaaacggt gattcaaaaa gatccatttg accaaacacg ggtcattatg
    15481 ggaattaatt catttaattt tggttggcaa ctaaaaactg gcgactcatt tcaaacgcca
    15541 gaagtcgtga tggtttatag taatgaaggt ctgaatacgt tatcacagac gtatcatgaa
    15601 ttgtataatc accgtttaat tcgtggaaac tatcgtttaa aagaacgtcc cacgttaatc
    15661 aacaactggg aagcaacgta ttttgatttt aacgaagaaa aaatcatgaa tattgtcgat
    15721 gatgcgaaga acttgggcat tgaaatgttt gttttagatg atggttggtt tggcggtcgt
    15781 gaaaatgact tcacttcatt aggtgattgg gttgaaacag aaggcaaact tgcttgtggt
    15841 ttggaaaatt tagccaagca agtgcatgat aacgggatga aatttgggat ttggtttgaa
    15901 ccggaaatga tttctgaaga tagtgaacta tttcgagcac atccagattg ggcattgggt
    15961 attccaggtc gtgaccgctc aattagtcga aatcaatatg ttcttgattt ttcaagacaa
    16021 gatgttcgtg ataatatcta tcaacaaatg acggcgattt ttgatcgtgt accgattgat
    16081 tatgtgaagt gggatatgaa tcgaaatatg acagaagtct attctgtctt gttggactca
    16141 acgatgcaag gtgaagtggc tcatcgttat atgttagggt tatatgaatt tttagaaaag
    16201 ttaacaacga attatccaac tattttattt gaaagttgtt cgggtggagg cggtcgtttt
    16261 gatgcaggtt tcttgtatta catgccacaa acttggacga gcgacaatac cgatgccgtg
    16321 gctcgtctga aaattcaata tggaaccagc ttagtgtatc caatttcaag tatgggtgcg
    16381 catgtttcag cagtcccaaa tcatcaaaca ggaagagaaa cgagtttaac tattcgtggc
    16441 aatgctgcga tggctggtgt ttttggttat gagctagatc taacgcaatt aacttctgaa
    16501 gaaaaagcag aaattaaaga acaagttgcc ttttataaaa agcatcgcca acttttgcaa
    16561 tatggaactt tttatcgctt ggaaagtccg tttgaaggca atgatactgc atggatgttt
    16621 gtctcaaaag accaaacaga agccatggtc ttttatttcc gagtattagc agaagctgcg
    16681 catccattag tcacattgaa attagcagga ttagcagaag aagcaactta tcaaatggaa
    16741 gatgtaacat ttgctggcga tgaattgatg aatctcggtt tttatattga ctcagaacta
    16801 catggtgatt atgcgacaca acgcttttat ttgaaacaga tatagtagct tgaatttaca
    16861 atataagtgc aacaccaaaa gagactcatt ccaaacgaag aaaatatgtt ttatttacac
    16921 gcatttacta aaaagcagcc tgatttgaat tgggaaaatg aagaagtacg tgaagaaatc
    16981 tatgcaatga ttaactattg gttagataaa ggactaggcg gatttagaat cgatgcgata
    17041 ttaaacatca aaaaaaaact agagtatggc atctttgaat ctgatggaga agatgggtta
    17101 gccttcattg ggccctggat tcttaatcag tcgggcattg aagtgtggtt aaaagaaatg
    17161 agagagcgga catttaaacc acataatagt atgacggtag cagaagccga tgttcctaat
    17221 gaacgtttag atgaatatat tggtgaagat ggctattatt cgatggtttt tgattttagt
    17281 tatacggata ttgatgtacc tgaaactggt gaatggttta aagatagcca atggacatgg
    17341 acggatatgc gtaccaatat ttttacgaat caattagtta cgcaagataa aggttggggc
    17401 gccttatatt tagaaaacca cgatcaacca cgttcaatta ataaatatat tccagaagaa
    17461 tggatcaatg attatagtaa aaaaatgttg gcgacgcttt ttatgttact aagaggtacc
    17521 ccttttattt atcaaggaca agaactagga atgaccaaca tagaaatggc atcattagaa
    17581 gattttgatg atgtagctac gcattctcag tataaacggg cattagaata tggattggca
    17641 ccagagcaag cattgaaagc tgtcaataaa cgaagtcggg ataattctcg cacacctatg
    17701 caatggaata cgcaaaaaaa tgccagattc tcaacttctg ataatgtatg gttaaaagta
    17761 aatcctaatt atccagaact caatgcagaa gctcagttaa ccaatcaaca gtcggtattc
    17821 aattattatc atcaattaat taaattaaga aaaaatagtg tatacaaaga cgtgctaatc
    17881 tacggacaat ttctaccagt gaaagataaa aatgaacata ttttgcttta tgaacgccaa
    17941 ttagaagata aaatcgtatt agttatctta aacgttacag caacagaaca agaatattat
    18001 gtagatgaac agtaccaaca agtattaatc aataattatg aagatgtttt attagatcaa
    18061 agtcgaaaat tgcgtttacg accgtttgaa agtattgtat tagctaatta tggaggacaa
    18121 ttatgaaaat taaaaacgaa gcaatgctca tcacctatgc agatagttta ggaaaaaaca
    18181 tgggcgaatt aaaacaagtc ttagacaaac atttacaagg tgttattggt ggtgtacatc
    18241 tgttgccttt ttttccatcg acaggtgatc gaggctttgc gccgagtgat tatacgacag
    18301 ttgatccagc tcttggaaca tgggaagatg tggaagcatt aggtgaagat tattatttga
    18361 tgtttgattt tatgattaat cacatttctc gtgaatcaaa atttttccaa gatttcaaaa
    18421 aaaaccatga gcagtcacca tacaaagaaa tgtttatccg tattcatgaa tttttcccag
    18481 aaaatcgacc aacacaagaa gatattgatt taatctacaa gcgtaaagac aaagcgccgt
    18541 ttcaaacggt agaatttgcg gatggtacga cagaagaggt ttggaatacc tttggggaag
    18601 aacaaattga tttagatgtg accaaagaag ttgtgaaaga atttattcga gaaaccatca
    18661 aagatatggc gaaccatggc tgttctttaa ttcgattaga tgcgtttgct tatgcgatta
    18721 aaaaattaga taccaatgat ttcttcgtgg aaccagatat ttgggaactt ttagacgaag
    18781 tgcgtcaaga agcagcgaaa tatgacgtcg aattattacc agaaatccat gagcattatt
    18841 ccattcaaat gaaaattgcc aatcatgatt attatatcta tgacttcgcc ttgccaatgg
    18901 tcactctgta ttcactttat agtggaaaat cagaacgttt agccaattgg ttgaaaatga
    18961 gtccgatgaa gcagtttaca acattggata cgcatgatgg aattggtgtt gtggacgctc
    19021 gtgatttatt aacagatgaa gaattggatt acacctctga ggaattgtat aaagtcgggg
    19081 cgaatgtgaa gaaggtctat tcaagtgcca attacaataa tttagatatt tatcaaatta
    19141 acagcaccta ttatagtgca ttaggcgata atgaccgtag ctatctgtta gcacgtgcga
    19201 ttcaagtatt tgcgccaggg attccacaaa tttattacgt tgggttatta gcaggaaaaa
    19261 atgatattga actgttggaa gcaacgaaag aagggcgtaa tattaatcgt cattactatg
    19321 atttagccga aattgaagaa gaagtgcaac ggccagtgat tcaacaatta tttgcacttt
    19381 taaaatttag aaatgaatct gcggcgtttg atttggatgg aacgattgaa gtagcaacgc
    19441 catcagaatc agaaatagtg attacacgta aaaataaaaa cggtagtgat agtgcaacat
    19501 taacggctaa cttggatacg aaagaattta tcattgagtc taaagatata aaaattttca
    19561 ctagtgttgc attaaaattt taacctattc catagaacaa aaaatccttt atactagcac
    19621 ttggatgact tatccaagac caataaaaag gactcataca tggataagta taaaacaaat
    19681 acagcattta ataaatggtt ttctgccata aaattaggaa aactttctgt caactcccaa
    19741 aaatgtattt ttgattacaa ttcgtatcgt aaaaaactgt cttttgaagc tgtgctcaag
    19801 ctctttctct acgcagttaa tgaagaaaaa gaaagtttgc gtgatttgag tacagctctt
    19861 atcgaccgat cgctccaact agaaaccgat gtgtcatcga ttagtcatac ccaattgtct
    19921 cgagcattta atgcacttga acctaaagtg ttagaagaaa tttttcaaca actgttagaa
    19981 aaagttcaac atcaaatcaa accaaccaag cgaaatcatc tgtatctgat tgattccagt
    20041 acgttttcgc ttagtttaaa acgtcataag tgggcgactt tccgtcaaac aaaatcaggt
    20101 gtcaagttac acttaaacta ttgttatatg gacgatacta cgatgtatcc aactgatttt
    20161 accttaacaa atgcccatga acatgatctc aatcaactac cagtattggt gaatcaacca
    20221 gaggcaactt acgtgtttga tcgtgggtat taggactttg aaaagatgga tcagatgcat
    20281 tgggatggct atttcttcgt cactcggatt aaaaaaaaca ccaaagttca cgtgatagat
    20341 actctggaaa catcgccaga aacagaaatt ctacgagacg agctggttcg tttaggatct
    20401 aaaacctatc taaccgccaa tttccgacta gtcactgttc aagataagaa cggacgtgtg
    20461 tttcaattta tcaccaatcg aatggacgtc tcttcaaaag aaatctctga tatgtatcac
    20521 gctcgttggc aaatcgaatt gtttttcaag cacatcaaac aacacatgac cattaaaacc
    20581 ttcttttctc aaagtgaaaa aggcgtgcag aatcaattga ttttaacgat gatttccgct
    20641 ctcctcacgt ttctcatcaa attggagacg aaaacggaaa aatcagtgtt ccaaatcaaa
    20701 cgattctttc gttatttact gtttcagccc tttgaatgtt gtctagaaaa attaattccc
    20761 acatgagaaa aaagaaattt ggctcgttac ttttggtttt cagttgaata acagataaaa
    20821 aattttctgg aaaaagtcat cctctgcgtc agcacagttt tttttgactt attttcagag
    20881 gaaatagttt tcagaatatt ctaaaatgtt ttatgcaaca ctagtgatat tttttagaat
    20941 tagatttcta atgtgcattt tttgagtatt aaggagcaag tatacaaaga tttataaagc
    21001 tgattaaatt tgaataaaaa tatgcaggaa cgctactatt tttagtagtg ttctttttta
    21061 tatttgaata aaaaatgtgg tggttaaatg gtggttataa caaaaaatga attaatgaaa
    21121 ttaggttatg ggaagtatca gtctgagaga atcatacgac aggcaaaagc aattatggct
    21181 tcaaaaagct atacctatta cgaaaataga agattaggac tagtacctgt tgaatctgta
    21241 gaagaagttt taggtatgaa gatagaggta gaggaggatt ttcagaatca tggttaattt
    21301 taaaaaagat aaaaatggga aatatcattt tactgctagt ttgggattcg acaaacgaac
    21361 agggaaaaga gtacaaaaaa acgtagaggg gttaaaacag ttaaagaaga aaaaaagcgt
    21421 ataccgaact tgtttctaat tatggaaaag aagcagaaac ttctcaaagt actatgttgt
    21481 ttgaggagtt tttttataaa ttattcctac cttattatca agggaaagta gaagaaagaa
    21541 catataaaaa tcggttatct acaatcaaaa aaacttttct tatttcttta aaatggagtt
    21601 aagagaaata cgtccaattg acattcaaaa gtggcaaaat aatttattga gcaaatataa
    21661 aaattcttat gtaagaaaca tgtatgggat ttttcagatg tcattagatc gagcagttgt
    21721 tttgggacta cttgaaaaaa atcctgcaaa agttattgga aatgtgaaga aaaggaaaac
    21781 tacagttgat ttttggacaa aagaagaatt tacaaaagtt atttcaactt tttatattga
    21841 tgatttgtac caacattttg gttttatttg tatgtggtta ctttttatga ccgttatgcg
    21901 tttgggagaa gccactgcat taacttggaa agatattgat tttgaaaata aaaacttgcg
    21961 tgtgaataaa acactctatt ataagagagc caaaacctat gagtttgtag aacccgaaac
    22021 ggcttctagt attcgtacaa tcgaactaga cgacaataca attacctttt taaaagcttg
    22081 gaaggagcgt caagtttcaa taggaggcat gtcgtttgta ctttcttata atggaacacc
    22141 tactcagaaa tatacaatcc gtcatattat gagaagacat gcaacgatcg ttggtgttca
    22201 tataatccgc atacacggat taaggcatag tcatgcttct ttgcttatat cacaataaaa
    22261 agatgcactt cgaattaaag aaaggttaaa acatgctgat gtacaaacga ctttaggaac
    22321 ttatggtcat ttgtatctaa attcattgag agagataaca aatgatccca cagattatat
    22381 ccaaactaat ttttctaaaa tcaaaccgac acctatttac gataataaaa aataaatgat
    22441 aaacaaaagt aggatcacac caaaagaaac acactagaaa aactataata caggcttttc
    22501 tagcgtgttg tttcttattt ccgcctattt atacattata ctcataaagc atttaatgca
    22561 tcgatcgtag ccgcattgtc tatatgggta tataaagttg ttgtattggt acttgaatgt
    22621 cccaattgtt gggcagttaa taccaatgaa tttgtctgtt ggtagagtct agtagctaat
    22681 gtatgacgca gtttgtgagg gctgacttga attctaaagc cttttgaata ttttgctacc
    22741 atccgttcga ttgcaatacc ggatatacgc ttggcttctc cttggtaaat tgctagaaat
    22801 aaagcttttt cgttttctgt tactttatat cgttctttcc gaatagctag ataattagct
    22861 aaatattcca ttgcgaatgc tgctatattg acagcatctc ttttatttcc ttttctagtt
    22921 actaccacat tattcttatc taaagatagg tcatctaaat taatatttat aacctcggag
    22981 agtctcaatc cagatcctaa aatcagtgca ttgatcgcca ggtctctttc tacatctcgt
    23041 tgataataga tcaaagctcg tttactcaaa gtatttttat attcattggc aatataatct
    23101 agataatttt gtgtatcctt atctagaaat aattttttct caatggcatt ggcacgtgca
    23161 gctaacgtag ttttatctgt gatggttgct acttgatcca taggattatt gtctaagtat
    23221 ggttttccta gaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgcc
    23281 gtgatatttc tattgatagt agaagtagat agacctaagt tattattatc tttgagctta
    23341 gtttttgttg tctctttttt tacattttct tttgtgcgac gtttcaaatg agatttgtac
    23401 aagtttaggt cataggcagt taactgtctt atcgaggtga tagaagtatc aggaacgata
    23461 gactcactca gaagccattc ataaaagagc ttataatttt ttagatattc taaagtggtt
    23521 cgaggtgatt gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata
    23581 tgaagtatga ttgtatctat tttttgatat atttctgtat ttttcatgtc attcatcctt
    23641 tcaataagta ggggagaaga gtatctctat aattggacta taccaattat agaatgtttt
    23701 gtcaattggt atgctctaaa atttattaat ttgtttcaaa aaaagcatat aaacaggtat
    23761 acgtttaata atttaaaata aatggtatac ataaataggg aaggaaatag aagggagcaa
    23821 tctaaatttt ttgaattttc ttaattaatt atatagaaag aacaatcttt ctatagagtt
    23881 agacatgaga gggactatgc ttgatttaaa atcaacaagc gtatatactg tatatacaaa
    23941 gaaaaaggag ggagaagact atggcaatta aagaaagaaa atttagggaa gtggggaact
    24001 cagtagtatt aactatttca aaggagtttc ttaaagaaaa tgggttaaat cccggagata
    24061 cggttttttt agatgaagaa aaacttcgag cggcaattac aaaaaaacaa gccttatctg
    24121 atgaaattga tttagcaata tctcaagcat taactgaata tgatactact tttagagagc
    24181 ttgtcgataa atgagtattc attacctaga agccaaagat attataaaaa tgaatgtaat
    24241 ccaaataaaa aaatactctc ctaatgaacc ccttggtgta aaggatgcga atgctttaga
    24301 aatgtgtgta gggcaacttc gagcctcagc ttttggtgaa gaggtttatc cttcgattta
    24361 tgaaaaagca gctatcctac ttatacaatt gataaagaaa catccttttc acaatgcaaa
    24421 taaacgtact gctttcttag ctacttttgt ctttttgaaa atcaatggac agcttttagc
    24481 tattgatcaa aaagatgctg taaatttagc ggtatatatt gctacgtatg ataaggattt
    24541 cgatcaacta aaatatgaag tgatcaacac tataaagaca catacgcagc ccatttaagg
    24601 agttactgta tgtgtcttta tagggttatt agctgctata gcgaaataat tgaagtaaat
    24661 tacaaatagc tataattcaa atccaactat atagaaaacg taaattttct atatagttgg
    24721 attaagataa cgactattta ttttatcata tttttactat accctatact tttacttggt
    24781 atatttatga ataatcgcaa atttacccct atactactag tttgtaagtt agattaataa
    24841 aaatactaaa gttatttgtt cttttattaa atacacctac aacttataaa ttgatggtat
    24901 ttagataaca cgaaagaata aaaataacag ctgatagtaa agtttgttga cgattgctac
    24961 tcaatcgaaa gtgagttagt gcgtatgaac ttgcttctga aaacttatga aatgaaagac
    25021 cttttacatc ttgcagaaat cgttactggg attaaactaa gcaataaaaa ctaatgttcc
    25081 cctcattctt tgaacttcta ctatttgatt tatttattct gataaatgac gatttcacct
    25141 tcaggatctt caaatcccgt atagaattta tttaagatct cttcagtatc agtaatagga
    25201 taggcgatag gccacatacc ctttgatggt tagaataaac tgctcaaaat ctgtatcgta
    25261 tgttggcttt agcatattaa caaaggctat ctccaaatta taaaccataa gcgttgaatc
    25321 gttcttaaca atttcaattc ttcctaaccc aaagtcagaa gatataatta ccggacttgc
    25381 cttacccttc atgtgggttg ttgagattcc atatttaaag ggaattgtcg gtactatcgg
    25441 cggtctgaaa aacatcccaa aaataaaaaa atctttcccc tgctagtctc ctaagtccct
    25501 ttgtagcgga tatggcacca taatctcatt ttatcgaagt gagtcatggt ttatctgttg
    25561 gccaaccaaa attgatacaa tctaacgatg caccctctac ggcattctag gggagtgcac
    25621 ttgcattcac ccaataaaaa ccggcagtta gcaaagaact tatgtagtcg tatgttctta
    25681 ctgttaacat acaagaggtc tcctttccac aattgcttag cagtaaacgg gaatttacaa
    25741 agctaatctc ttccattttc ttatctttgt tctaaaagtc ccaaatggag caactgtatt
    25801 tacatgaata aatttatata cttcccatac agccgttttg gttgcctcgt ctgcccattt
    25861 tcttttgtgt gcagtgaata gatcttcatc actcattgaa tcaatcatca tgttaatatc
    25921 atcaatatta cgattgagaa tttcctctaa ttctttcaat ggtagatgtg cataggtatt
    25981 tgtaaaccac ttatataact ctcctaactg attccattta aattgatcag aaggcgtttt
    26041 aacagataat ccatttcttt catcattttc ccattttaga attaaattcg tccaaccaac
    26101 ttgatatgct aaattttcag caggtgttct atccacttcc tcaactcttt tatccttcaa
    26161 ttcttcagga atagtttcaa attctaaaat atatttagta taagtcctat ttatttcttt
    26221 ttttaattct tctttatttt tatatgttct cattatatta cctccataaa ttcctaattg
    26281 tctcctttaa ttcatctata atttcatcca cgatatttta atccgttcta caatgcatat
    26341 ctgatatgca ttgactgtct aactcatatg tgaatgattt ataaaatatc ccaacgtctc
    26401 atctaaagaa atgttgatcg tcagcaagga gctaaaaaat acgaatatat ttcttttgta
    26461 gtaaaatcgg actggtttgc atatcatttt cttcgtaaat atcaaaatca tgttaactac
    26521 tatcaattac taataaaaac taccacttta agcgttaaga actgttaagt ttagttaagt
    26581 gaggatagtt cacaaaatcc attgttaaac ttcacgaaag tggtaaaact caaaatgctt
    26641 tatataggat tacagcatcg ctctttcttc gattgcgcgt tgggtaaaaa atattatgaa
    26701 gtaaaaattg acgatgacac gttccttacg gctcatcaga tgaaacagct acaaaaacga
    26761 aatgatcaac tcgaagaaaa tttaattcta aaaaagcgat tgccatattc acgcctcgct
    26821 cgacgaacta ttaaaggtca agatttacct gaagatcatc ctttttagga aattgaagct
    26881 ttttatgttg cacaatgcat ttataatact acaatgatgt tttcaccaga tattcttcta
    26941 gggtgcggtg taatgaaaca gatacacttg caaaagaaaa ttcaccgcga atttactaaa
    27001 attatgaata attatgttaa tcttccgaaa tttttttcca aaattaaggg atcaagctgg
    27061 tgttatagat tgtttaaggt tggcaaaaaa ataacttctt tttaaacaat tatagaaaac
    27121 cgatactctg ttaagttggt tgacacggtg tcgattttta tgactttctt attctgaaac
    27181 ttcactaacg gacagtacct aaaaagtcta tagtatggac gtttacgagt actattttat
    27241 caaaaatgct gctcatttca acttatatgt tttagtgaac tacccccact ta
//
LOCUS       DAZAFF010000032        26981 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000032 DAZAFF010000000
VERSION     DAZAFF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.032"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(151..2304)
                     /locus_tag="ACG9BB_001296"
     CDS             complement(151..2304)
                     /locus_tag="ACG9BB_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFN2837244.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2598..3446)
                     /locus_tag="ACG9BB_001297"
     CDS             complement(2598..3446)
                     /locus_tag="ACG9BB_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN2837245.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="ACG9BB_001298"
     CDS             complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="ACG9BB_001298"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFN2837246.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5947..6126)
                     /locus_tag="ACG9BB_001299"
     CDS             complement(5947..6126)
                     /locus_tag="ACG9BB_001299"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFN2837247.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6173..7996)
                     /locus_tag="ACG9BB_001300"
     CDS             complement(6173..7996)
                     /locus_tag="ACG9BB_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN2837248.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8274..10553
                     /gene="helD"
                     /locus_tag="ACG9BB_001301"
     CDS             8274..10553
                     /gene="helD"
                     /locus_tag="ACG9BB_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFN2837249.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10646..11293)
                     /locus_tag="ACG9BB_001302"
     CDS             complement(10646..11293)
                     /locus_tag="ACG9BB_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837250.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11472..12191)
                     /locus_tag="ACG9BB_001303"
     CDS             complement(11472..12191)
                     /locus_tag="ACG9BB_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2837251.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12322..13473)
                     /locus_tag="ACG9BB_001304"
     CDS             complement(12322..13473)
                     /locus_tag="ACG9BB_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFN2837252.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13690..14430)
                     /locus_tag="ACG9BB_001305"
     CDS             complement(13690..14430)
                     /locus_tag="ACG9BB_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN2837253.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="ACG9BB_001306"
     CDS             complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="ACG9BB_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFN2837254.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14814..15407)
                     /gene="yqeK"
                     /locus_tag="ACG9BB_001307"
     CDS             complement(14814..15407)
                     /gene="yqeK"
                     /locus_tag="ACG9BB_001307"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFN2837255.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15391..16041)
                     /locus_tag="ACG9BB_001308"
     CDS             complement(15391..16041)
                     /locus_tag="ACG9BB_001308"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFN2837256.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="ACG9BB_001309"
     CDS             complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="ACG9BB_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFN2837257.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="ACG9BB_001310"
     CDS             complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="ACG9BB_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFN2837258.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17484..18011)
                     /locus_tag="ACG9BB_001311"
     CDS             complement(17484..18011)
                     /locus_tag="ACG9BB_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFN2837259.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18191..18976)
                     /locus_tag="ACG9BB_001312"
     CDS             complement(18191..18976)
                     /locus_tag="ACG9BB_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFN2837260.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18969..19838)
                     /gene="accD"
                     /locus_tag="ACG9BB_001313"
     CDS             complement(18969..19838)
                     /gene="accD"
                     /locus_tag="ACG9BB_001313"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFN2837261.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19839..21218)
                     /locus_tag="ACG9BB_001314"
     CDS             complement(19839..21218)
                     /locus_tag="ACG9BB_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFN2837262.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="ACG9BB_001315"
     CDS             complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="ACG9BB_001315"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFN2837263.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21670..22146)
                     /gene="accB"
                     /locus_tag="ACG9BB_001316"
     CDS             complement(21670..22146)
                     /gene="accB"
                     /locus_tag="ACG9BB_001316"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFN2837264.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="ACG9BB_001317"
     CDS             complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="ACG9BB_001317"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFN2837265.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="ACG9BB_001318"
     CDS             complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="ACG9BB_001318"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFN2837266.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="ACG9BB_001319"
     CDS             complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="ACG9BB_001319"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFN2837267.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25176..25400)
                     /locus_tag="ACG9BB_001320"
     CDS             complement(25176..25400)
                     /locus_tag="ACG9BB_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFN2837268.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25453..26415)
                     /locus_tag="ACG9BB_001321"
     CDS             complement(25453..26415)
                     /locus_tag="ACG9BB_001321"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFN2837269.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26425..26865)
                     /locus_tag="ACG9BB_001322"
     CDS             complement(26425..26865)
                     /locus_tag="ACG9BB_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN2837270.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         7364 a         5988 c         4690 g         8939 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     2341 tgtacccaaa aacttcttaa taaaaaagaa aaaatgggcg gagcaagaaa ttctttacct
     2401 aatttcaaga taaaatcaac tttttgtttc acagcaatca cttaaagtta catatccaac
     2461 tatttttttc attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa
     2521 cactttgatg gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat
     2581 aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt
     2641 tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg
     2701 atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt
     2761 ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc
     2821 gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct
     2881 gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt
     2941 tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat
     3001 ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc
     3061 tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact
     3121 gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac
     3181 tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc
     3241 gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc
     3301 ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca
     3361 atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata
     3421 ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta
     3481 agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat
     3541 ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt
     3601 tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa
     3661 atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa
     3721 gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc
     3781 atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc
     3841 acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca accccttcat
     3901 ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat
     3961 gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa
     4021 ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg
     4081 cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc
     4141 taattgtata gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag
     4201 ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc
     4261 catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt
     4321 atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc
     4381 agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg
     4441 acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa
     4501 agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc
     4561 acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg
     4621 aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg
     4681 atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg
     4741 ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt
     4801 tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa
     4861 gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat
     4921 atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg
     4981 attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat
     5041 acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata
     5101 agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact
     5161 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
     5221 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt
     5281 ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg
     5341 gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac
     5401 taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat
     5461 tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa
     5521 gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac
     5581 tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg
     5641 atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg
     5701 acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa
     5761 cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc
     5821 tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt
     5881 tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa
     5941 ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat
     6001 gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc
     6061 tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa
     6121 tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta
     6181 tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac
     6241 tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta
     6301 gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact
     6361 gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat
     6421 catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca
     6481 ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca
     6541 tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca
     6601 agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag
     6661 atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg
     6721 tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat
     6781 caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg
     6841 cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt
     6901 ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag
     6961 ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt
     7021 ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg
     7081 ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta
     7141 caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta
     7201 gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg
     7261 agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac
     7321 gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa
     7381 tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc
     7441 catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac
     7501 ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta
     7561 caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg attactatat
     7621 cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact
     7681 gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca
     7741 tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg
     7801 taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa
     7861 acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct
     7921 ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt
     7981 ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa
     8041 atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata
     8101 ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac
     8161 aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa
     8221 aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg
     8281 agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa
     8341 atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca
     8401 cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat
     8461 atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac
     8521 gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag
     8581 aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag
     8641 aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg
     8701 gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac
     8761 aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg
     8821 atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct
     8881 ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga
     8941 ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt
     9001 tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt
     9061 tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac
     9121 agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc
     9181 aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat
     9241 tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt
     9301 tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt
     9361 ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc
     9421 tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc
     9481 tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac
     9541 aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc
     9601 gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact
     9661 ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc
     9721 aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg
     9781 ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc
     9841 gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag
     9901 ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa
     9961 atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc
    10021 aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg
    10081 tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag
    10141 gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa
    10201 aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt
    10261 atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact
    10321 ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc
    10381 gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc
    10441 tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa
    10501 gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa
    10561 gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa
    10621 aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat
    10681 attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg
    10741 ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat
    10801 tccattcaat gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca
    10861 aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt
    10921 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
    10981 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
    11041 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
    11101 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
    11161 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
    11221 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
    11281 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
    11341 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
    11401 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
    11461 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
    11521 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
    11581 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
    11641 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
    11701 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
    11761 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
    11821 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
    11881 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
    11941 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
    12001 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
    12061 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
    12121 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
    12181 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
    12241 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
    12301 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
    12361 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
    12421 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
    12481 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
    12541 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
    12601 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    12661 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    12721 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    12781 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    12841 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    12901 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    12961 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    13021 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    13081 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    13141 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    13201 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    13261 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    13321 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    13381 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    13441 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    13501 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    13561 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    13621 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    13681 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    13741 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    13801 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    13861 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    13921 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    13981 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    14041 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    14101 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    14161 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    14221 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    14281 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    14341 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    14401 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    14461 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    14521 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    14581 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    14641 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    14701 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    14761 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    14821 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    14881 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    14941 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    15001 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    15061 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    15121 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    15181 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    15241 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    15301 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    15361 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    15421 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    15481 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    15541 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    15601 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    15661 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    15721 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    15781 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    15841 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    15901 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    15961 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    16021 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    16081 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    16141 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    16201 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    16261 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    16321 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    16381 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    16441 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    16501 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    16561 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    16621 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    16681 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    16741 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    16801 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    16861 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    16921 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    16981 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    17041 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    17101 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    17161 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    17221 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    17281 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata
    17341 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    17401 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    17461 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    17521 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    17581 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    17641 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    17701 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    17761 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    17821 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    17881 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    17941 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    18001 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    18061 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    18121 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    18181 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    18241 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    18301 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    18361 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    18421 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    18481 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    18541 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    18601 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca ataagcacct gctgtattga
    18661 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    18721 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    18781 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    18841 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    18901 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    18961 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    19021 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    19081 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    19141 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    19201 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    19261 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    19321 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    19381 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    19441 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    19501 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    19561 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    19621 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    19681 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    19741 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    19801 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    19861 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    19921 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    19981 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    20041 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    20101 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    20161 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    20221 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    20281 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    20341 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    20401 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    20461 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    20521 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    20581 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    20641 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    20701 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    20761 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    20821 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    20881 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    20941 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    21001 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    21061 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    21121 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    21181 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    21241 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    21301 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    21361 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    21421 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    21481 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    21541 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    21601 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    21661 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    21721 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    21781 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    21841 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    21901 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    21961 ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    22021 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    22081 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    22141 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    22201 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    22261 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    22321 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    22381 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    22441 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    22501 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    22561 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    22621 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    22681 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    22741 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    22801 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    22861 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    22921 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    22981 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    23041 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    23101 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    23161 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    23221 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    23281 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    23341 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    23401 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    23461 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    23521 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    23581 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    23641 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    23701 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    23761 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    23821 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    23881 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    23941 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    24001 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    24061 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    24121 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    24181 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    24241 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    24301 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    24361 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    24421 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    24481 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    24541 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    24601 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    24661 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca
    24721 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    24781 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    24841 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    24901 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    24961 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    25021 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    25081 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    25141 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    25201 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    25261 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    25321 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    25381 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    25441 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    25501 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    25561 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    25621 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    25681 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    25741 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    25801 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    25861 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    25921 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    25981 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    26041 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    26101 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    26161 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    26221 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    26281 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    26341 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    26401 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    26461 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    26521 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    26581 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    26641 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    26701 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    26761 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    26821 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    26881 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    26941 caaccttaaa agatcatttt tttactatat gagctttttt t
//
LOCUS       DAZAFF010000033        25548 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000033 DAZAFF010000000
VERSION     DAZAFF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.033"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            173..2233
                     /locus_tag="ACG9BB_001323"
     CDS             173..2233
                     /locus_tag="ACG9BB_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFN2837271.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2316..2885
                     /locus_tag="ACG9BB_001324"
     CDS             2316..2885
                     /locus_tag="ACG9BB_001324"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2837272.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3060..3866)
                     /locus_tag="ACG9BB_001325"
     CDS             complement(3060..3866)
                     /locus_tag="ACG9BB_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837273.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4004..5518
                     /locus_tag="ACG9BB_001326"
     CDS             4004..5518
                     /locus_tag="ACG9BB_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFN2837274.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5921..6904)
                     /locus_tag="ACG9BB_001327"
     CDS             complement(5921..6904)
                     /locus_tag="ACG9BB_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFN2837275.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6909..7556)
                     /locus_tag="ACG9BB_001328"
     CDS             complement(6909..7556)
                     /locus_tag="ACG9BB_001328"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFN2837276.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7641..8273)
                     /locus_tag="ACG9BB_001329"
     CDS             complement(7641..8273)
                     /locus_tag="ACG9BB_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN2837277.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8289..8840)
                     /gene="coaC"
                     /locus_tag="ACG9BB_001330"
     CDS             complement(8289..8840)
                     /gene="coaC"
                     /locus_tag="ACG9BB_001330"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFN2837278.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8837..9574)
                     /locus_tag="ACG9BB_001331"
     CDS             complement(8837..9574)
                     /locus_tag="ACG9BB_001331"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFN2837279.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9571..10560)
                     /locus_tag="ACG9BB_001332"
     CDS             complement(9571..10560)
                     /locus_tag="ACG9BB_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFN2837280.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10818..11360
                     /locus_tag="ACG9BB_001333"
     CDS             10818..11360
                     /locus_tag="ACG9BB_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFN2837281.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11377..12462
                     /locus_tag="ACG9BB_001334"
     CDS             11377..12462
                     /locus_tag="ACG9BB_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2837282.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12474..13280
                     /locus_tag="ACG9BB_001335"
     CDS             12474..13280
                     /locus_tag="ACG9BB_001335"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2837283.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13281..14108
                     /locus_tag="ACG9BB_001336"
     CDS             13281..14108
                     /locus_tag="ACG9BB_001336"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2837284.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14105..15178
                     /locus_tag="ACG9BB_001337"
     CDS             14105..15178
                     /locus_tag="ACG9BB_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2837285.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15606..18302
                     /locus_tag="ACG9BB_001338"
                     /pseudo
     CDS             15606..18302
                     /locus_tag="ACG9BB_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            18367..18867
                     /locus_tag="ACG9BB_001339"
     CDS             18367..18867
                     /locus_tag="ACG9BB_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2837286.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            18879..19373
                     /locus_tag="ACG9BB_001340"
     CDS             18879..19373
                     /locus_tag="ACG9BB_001340"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2837287.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19394..20203
                     /locus_tag="ACG9BB_001341"
     CDS             19394..20203
                     /locus_tag="ACG9BB_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN2837288.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20200..21021
                     /locus_tag="ACG9BB_001342"
     CDS             20200..21021
                     /locus_tag="ACG9BB_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN2837289.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21072..23171
                     /locus_tag="ACG9BB_001343"
     CDS             21072..23171
                     /locus_tag="ACG9BB_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFN2837290.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23168..24286
                     /locus_tag="ACG9BB_001344"
     CDS             23168..24286
                     /locus_tag="ACG9BB_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFN2837291.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24325..25203
                     /locus_tag="ACG9BB_001345"
     CDS             24325..25203
                     /locus_tag="ACG9BB_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFN2837292.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25349..25546
                     /locus_tag="ACG9BB_001346"
     CDS             25349..25546
                     /locus_tag="ACG9BB_001346"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFN2837293.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8075 a         4449 c         5408 g         7616 t
ORIGIN      
        1 aacaaaatta aaaagtacat aagttattat tccgattttc ttgaaacaat aaaaaatcaa
       61 aaaaaatact ctcctaatag tccatcttct tttgatttta tttatataat gaaatcatct
      121 tatgaaaatt actacttcat gtttagacaa gagtagggag gactaagaat aaatgtgttc
      181 aaaaagacgt tcaaaacaac taaataaaca cgcttgtcgt ttagataagg aacggtcgtg
      241 ttctgaaaaa cactacgtta agtttatgat gcgcacgatg ctgatcagtt tatctgtagg
      301 tgtctatatg catgtacccg ttcaggcaac gacacaaacc atcaaggtcg tacgtacctc
      361 tgtttctgag accactgatt cccctatcac tattactcat actttcactg atgcaaatgg
      421 aaaacagcta cttcctgcat tgacttccta taataaagtc agttatcctc caacgataaa
      481 ccactactgg ttaattccta acaaaacatt atggacatcg gaaaccggaa gcagtgtgcc
      541 gctttctatc ttttccgata tgactgatcc tacaaaaggt gatcagttaa aacagatcac
      601 tgatattttg aatcatgctt tgacagatat aaaggacggt ggaagcgcac aagtttctta
      661 tgtatatgaa aaagattatt cacagtttga aagaaagaaa atcgatgttc tggcagggac
      721 aaaagtttcg atatctgatc tggtagaaag gctgaagaac gcagatggaa cacaaggaga
      781 tatgtccaaa gtaatttcaa acaatgggaa tatcgtggat acgactaata caggaacatc
      841 tactatttcc ttgtcctatt ttgatccaat tgctttaaaa acaatgactg catctgctgt
      901 gatccatgtt cgtgctgatc aaacagctat caaaggaaaa gatcttcact taagaacagg
      961 agagagttac gcccctaaag atcttgttga atatgtaaca aaaagcgatg gaacttcagg
     1021 aaataaagaa gaaatcaaaa tgaacgggac agtagatact acaactccgg gagaatatcc
     1081 cgttgtatta acttatatag actcgaaaac atcaaatgtg gcagaaacaa cagctgtagt
     1141 ttatgtagaa gcatctgctg cttcatcgac tgtcatcact cattttatag atagtcaaac
     1201 aaataaagaa cttcaagcag aaacacatat gagtagtgta aatcgtattc ctgatttgcg
     1261 cctacctgct gaattttctg atcgagtgcc aaatatagat ttatcgactt atgaacaaaa
     1321 tgggaaagtc acttctattt ctgattggat gcaagcaaac ggattagaca gaaaaagtca
     1381 ctggtcggag atccttgaag tacttcactc agaactgatg aataatagga agcaatcagg
     1441 aggggaaatt acctttactt atgtttatat accagacaat cgttcattag aaggtcatga
     1501 tcttattgtt catccaaacg aaaaaataaa caaagaagag ttgatccaat cagcaacaaa
     1561 ttccttaggt aaacccgtac cgttaaagga tgtatcggtt acaatcaatg aagtaccatt
     1621 ggaggatggt tatttgacca aacaaacagg tgattttacg attatgtata gttatcatga
     1681 cccgtattca ctccaagaac tccatgctca gtcactatta catgttttac cattaaacaa
     1741 acagatagat caaaaagaac aaaaaagaca agaagaaagc ttgtcagaaa aaaaacaaca
     1801 aatggctcat gcagtttctc caacagggaa agtaaaacaa acaagaagcc tagagttagc
     1861 aagaatatct agaaaaatct cagaatctaa taaaacgata aggcaagaag ctcatttggc
     1921 cccacaagtt tctggattgg aaaatgaaat tttagagcct aaaataaaac aacaatcaga
     1981 aaaagaattt gttgctaaac agtcggaaga tcagaaggaa tcagatgacc agaaggaaac
     2041 gaagaaaaaa cgcttgtcaa ctattgattc caccgatgag catctgcagc cgcaagtacc
     2101 acttcctagt ccgggaacac ccgcacctgg tggaggtatt ccttcttctg ctttaggaga
     2161 tttcatgcga ggtatggcag gaacatgggt atacgctaat cgaaatgaat ttgacgcaga
     2221 tgactggttc tgatttatat ctctatctgt tatagtcaaa taagtagctg agtcatatgt
     2281 tatttggcat aatggagacg aggagggata gaaaaatgtt agataaaacg ttaccttacg
     2341 cagaaatctg gatgacaaga ccacttcaac aaaatgttcc aatcacgcct ttagcggaag
     2401 gttttgaatt tcgattttat caaaaagaag atgaatctgc ttgggcggag attgagactt
     2461 ccgttgggga atttcaaacg acagatgaag cacttgagta ttttcaacaa aactttgctc
     2521 cttatcctga agaacttgaa agaaggatgt tttttgtaga gacaaaaaaa ggagaaaaag
     2581 tagcaacctg taccgcttgg cgaaaaaaat caagagagca agtgtatccg gtttttcatt
     2641 ggttagcagt gaaatcagat taccaaggaa aagggctcgc aaaagcattg actgccagag
     2701 tcttgcaaga atttcctacc ctccatacgg acggaccaat ctatcttcac acacagacat
     2761 ggagccatca ggcgattgct ctttataaaa aaatgggttt tacttttatt gctgaaaatc
     2821 tagacggcac acctaatcca gattatgaaa aagttatgca ggttttaagt aaattagata
     2881 aataacgaaa tggtgaaata agtcctcagt attgggataa aaaacgaaat agtcattcct
     2941 catatcttac ttttttgagt taatactatc ggataactaa tggcatgcca tacattcaaa
     3001 aaagaagggg cagtgacaaa agtattgtca ctgccccttc tcttgttttt tagcatgttt
     3061 tatcgtttga tttttttagg aataaaattc ttgatgaacg taaatttttg gtaatcgttt
     3121 cgactataga ggtaaccaaa tacactgaaa acaactgctc caataaaatt aacaaataaa
     3181 tccttcatag tatccatgat tcccacgtct aagtaaccat tttggatgat tgttttatct
     3241 gttttaccat ctgcattttg actttcaatg attgtccgag tgatatctgg aacatgatag
     3301 acagtgtttc cgtctttact taactccaca ctattgattt gctggatgat cgtatctttt
     3361 tgcatatcga attgcagtaa tttatctgct gaaaattcca caaactccca cataacacca
     3421 actgtcatcg aaaaacagaa ggaaacaact gctagtaata aaggagtcaa gttcatggtt
     3481 tttgtatttt tattgagcaa ataaacaagg gaaaagccga cacccccagc taaaaatccg
     3541 ctgaatgcat gtagaaaagt atcccacatt gaaaaatagc cataaaaatc acttaactct
     3601 cctaaaactg ttgaactaaa aataaaagat aacaaaatga tttctagcat atttggaatc
     3661 tgtatcttta atatcttttc cagaaaataa gggatttcaa ataaaattaa tgtcagtaat
     3721 aaaagcattg tatttcccca gtgttggttc cagacctgat ccactagcat aataacgacg
     3781 atgatacgta ataaaatata gattccgttt gtgattctac ctgcagtttt tgtactgttc
     3841 gtaaaaaagg ttttcaaatg attcataaaa taactccttc atgtatatcc ttaattatac
     3901 taagaagcgt gtgtttacca aagctatatg acttaaagaa aaacagagtt gatattgtga
     3961 aaaaaatcac ttaatatagt ataaggagaa aagggggata caaatgaaaa aattagttgc
     4021 tggtttatta acagccgttg cacttcttgc tatgagcgca tgcggcgcag atcagaaaaa
     4081 tacagcgcag acaagttcta gtacgaagga atctgccgaa gtatcatcag gcgcttctga
     4141 acaagtatat acagatccaa aagaaatgaa agaatcttat gatgtcgtga ttattggagc
     4201 tggtggtgca gggatggcag cagctatcga agcaaaagat gctgggttga atccagtaat
     4261 tttggaaaag atgcctgttg ccggaggaaa cacatctaaa tcatcaagcg gtatgaatgc
     4321 ttcagaaaca aaatttcaaa aagaagaagg aatcaaagac tcgaatgatg ccttctatga
     4381 agaaacgcta aaaggtggtg gaggaacgaa tgataaagaa atgcttcgat actttgtcga
     4441 tcactctgct gatgcggtcg attggctaga tcaaaacggt atcaaactgg acaacttaac
     4501 gatcactgga gggatgagtg aaaagaggac acatcgtccg tctgatggct cggctattgg
     4561 tggatactta gtggaggggt tattgaataa cgtccatgag cgggaaatcc ctatttttgt
     4621 caacgcagat gtgacagaca ttaagaaaaa tggggaaacg gtcaatgaag tgactgtaca
     4681 tgttcaagga gaagaaccaa aagaggtcac cggaaaagca atcgtagtta ccacaggcgg
     4741 ttttggtgcc agcaaagaat ttattgaaaa atatcgtccc gatttagctg attatattac
     4801 gacaaatcaa gaaggatcaa ctggagatgg tatcaccatg attgaaaaat taggtggaca
     4861 aaccgttgat atggataaaa tacaaattca tccaactgtg cagcaggatg agggggtctt
     4921 gattggtgag gttgttcgcg gagaaggagc aatcttggtc gatcaagagg ggaaaagatt
     4981 tgtcaatgaa atggatacaa gggataaagt atctgcagca attacagcat tgcctgaaaa
     5041 atcagcttat ttgatttttg accaaggcgt gcgtgatcgt gccacagcta ttgaatttta
     5101 tgaccaaaaa ggatatgtca caactggaaa gacaatggaa gaattagcag aaaaaatcga
     5161 tttaccaaaa gaaacactag caaaaacagt tgatggatgg aatacggatg ttgcggagaa
     5221 aaaagatgct caattcaacc gtgccacagc catggaacat gacttatcgt ctccgaatta
     5281 ttatgcgatc aaaatcgcac caggaatcca ttacacgatg ggcggtgtga aaatcaatac
     5341 gaacactgaa gtattagata aagaaaacca gccaattagg gggttatatg ctgctggtga
     5401 agtcacaggt ggcttacatg gaaacaaccg gatcggtgga aattcagttg cggagatcat
     5461 tatttttggg cgacaagcgg gcatacaagc agctcgattt gcttcggaaa atgaatgaaa
     5521 tagaacgtaa ataaaaagag gttgtgaaat cagcgttttg agctaatacc gagggtcagc
     5581 tgattgaaca ctgtttagtg aagaggttgt gaaaaagctg ccctgcatca agtaataaga
     5641 acagccaagt aaaaacagct tcttatgttt ttaacttggt tggacttatt accgcagatg
     5701 caagcttttg aacaccgttt attcggaaaa agagcgtggg acaaaagtaa agaacacttt
     5761 tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gaaattcctt cggaaataag
     5821 ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt
     5881 aaacgcttct gtcccagcct cttttttgat cagcatgatt ttattcagtt atcttcaaaa
     5941 tgatttttcc gatgtttttg tttgcttcca tatattcatg cgcttgtttg actgcgctga
     6001 attggaatgt acggtcaatg attggccgaa tcttgccatt ttctagaaaa ggcccagctt
     6061 tttgcataaa agtttgtgcg agctctgctt tatactgatc acttctaggt gtcaataatg
     6121 taccaagcag ttggacacgt ttcccaatca atacaccaag gtctatctca ggcacgatgg
     6181 tcccacctag cataccaatc aagacccaac gtccatcaac ggctatagaa gctaaattct
     6241 tttcccagta agaagcgccg atgaaatcca aaataacatc gactccccga ccttcagtga
     6301 tttttcgtac ctcttcttga aagtcttgtt ttttatagtt gatcaattca tctgcaccaa
     6361 gttctttaca aaaggcgagt ttttcttctg atcctgcagt aacgatgatt ttcgcgtgag
     6421 aaagctgtct agctaattgg atcgctgctg ttcccacacc acttgctcct gcatggatca
     6481 agacagtttc gccttcttgt aattttccaa gccagtaaag tgtttgataa gcagtcaaaa
     6541 aaacttctga gattccagca gcagaaacat agtctagcgt atcagggaga aggattgcac
     6601 gatcggcagg catcacagca tattcggcat aaccacctag attgactaat ccgtatactt
     6661 ttgtcccagg agtaaaagtg gagatatcac ttttattctc aactacctct ccagctactt
     6721 cgactcccaa aatagggtag ggaggtttca aagagactga ttctcttttc ataatatctg
     6781 ttcgattgac agctgctgta tggactttga tcagtaattc gccttgtttt ggtttaggaa
     6841 caggcacttc aatttgttct aatacatcta cagaaccagg atgcttgatt gaaatggcac
     6901 gcatggatct attcctcctt tgcccattct tggaatgctg tattcaactc tttgctacct
     6961 tcagaaagta ctagagtcgt acgagaaata tccgtttctt ccactagagc ggcaagtaca
     7021 gcagcaactg tagagcgagt gatcgttttt gatgccttct cattaggctt agttaattga
     7081 atggaagtaa cttcttcatc gttagtcaat gtgactggtt gaagaatgac aaaatctaag
     7141 ttagttcgtt tcagccattc atctgcataa tgtttagtaa tataataatc tgtcatggac
     7201 tcaggccatt ttgctggttc atctgcataa acagcactga ccatcagata gcgagagaca
     7261 tgcgcatttt cagctgcgat cactgttttg attgcgccat ccaagtctac ttgcagaaga
     7321 ttctttcctt gagagccggc agcaaaaatc aatacatcga tgcctttgaa tgcttcggtc
     7381 attttttcca cggaccaagt taaatcaaaa gagacatacg aaacattctc tttttttact
     7441 aatgtttggg aagcaacatc tctcacccct gcaaaaattt catgggaact tggagctaaa
     7501 tcttttatca ggtgtcggcc aatttgtccg tttgcaccta caacaaaaat tttcatttgg
     7561 atcatccttt cgacgtcaaa tctttccatt caatagtaac accaaaagag aaaaaaatca
     7621 gagatcggct acgatgacta tcagttagta agagaagggt gcttgaagta tcttaacaag
     7681 acattgcata ctgctgcagt cgcaagacct gcgacgattc cttcaggtat accacttgta
     7741 aaaataaccg ctagaatagc tgaataaaca gcatttaaat ttgttccgat gactttggca
     7801 taatcttgct ggaataaaaa gtagatcata ttcatcacta aaatcgtatt ggtcaatgaa
     7861 ccggaaagtc cagctaagaa taaagaaacg gaatctgctt tttgtttcat tagtttgcgc
     7921 agacctttat aaacgaaata aggaaccacc ccaatcaaga tgcgaggaat aaacgcgatc
     7981 agcaaggctt tccagcttcc atgttctgtt ccgatgactg ggataaacgg agaaaagaca
     8041 aaagacaaag gtgtttggat aactgtgcta cggatcatgc tgatcagtcc aaatgtaccg
     8101 ccaagaaaag cacctagtct tggtcctaaa acgatcgaag caatgattac aggaatatgc
     8161 atggttgttg cattgattgg accgataggg ataaaaccta aaggcgtaac agctaataaa
     8221 atcaaaatag ctaaaaacat cgcagttaat gtaaagtttt ttgtattttt cataagtact
     8281 ccttttttct aagcatttct ttcattaaga gtcagcatga cctgttcgat gatcgtctga
     8341 atatcagcta aggcaccttt tccaaaatct ccacaagcta aaagagattc tcttggttca
     8401 atttcctgat agccgacaga tctcaatgtt tccagattac gctgagtgat cgggttttga
     8461 tacatattcg tgttcatggc aggggcaatc aattttggtg ttcccactgg aagcgccatt
     8521 gcgactgttg aaagaagatc gtctgcaatg ccgtttgcta gcttgccgat aatgtttgct
     8581 gtagcaggcg cgatgagaaa caagtcagct tgttttgcta gttcgatatg attgattacc
     8641 tgcggatttg attcttgcat gacgtctgta tggactgcgc gcttcgacag agattgcaag
     8701 gttaatggcg tgataaaatg tgtgctgctt ttagacataa tcacatctac ctgggcacct
     8761 aatttgacga attgacttgt aagatcagcg cttttgtaag cagaaatact tccagtaacg
     8821 cctagtaaga tctttttcat tttgattcct ccagtctaat tttctcagta atcaatgcag
     8881 caatctcaga tttactttga gcttcttcca ctgttccatc tttagataag agatagccgt
     8941 ggtgtgtctc gccatggatt tccgtcaaat catttgcgaa aataaaatca gttttgttat
     9001 ttatcagact attttttgcg actgccagaa gagattcctt tggtacatct actaatagtt
     9061 tgaaaccaat gatttttgct tttggctgcc attcacgtag ataagaaatg atttttggtg
     9121 ttttcttcaa aacgagcaca agatgctcgg tgttagaaga gatctttgaa acagtggtat
     9181 ctgtctgttc attttccgca aaccaattgt ccagttcttc ttgcgttgaa gaagtgggta
     9241 ggttttttgc cagctgttct tctgaaaaac taaaagcagg agtaaaatca ctgactgcca
     9301 tactgtggat aatcgcatcg tagtgttctt ttttcgttag ttcttctaat tgtaaaaata
     9361 agtccagtgc agattcgatc gtataaatcg ttatattgga gtgtcttttc ggtttcagtg
     9421 cttgtttcgt agtcacgtaa tcgattgtgg tatccggatt ggcagctaaa taatcagcta
     9481 atgcttgccc taatcgtcct gtcgaatgat ttgtaatagc acgtacttga tcgatttttt
     9541 cagtcgttcc tccagctgtg attagaatct tcatttatgg tcacctacat ttactagtgt
     9601 tcttccttgg tgtgttccag caagtagttt agatgctgtt tcaggcaatt gatccagagt
     9661 aatctcttgg acgaccagtt catctgcaat ctgcaaatcc gccatctgtt gccataaaga
     9721 aagacgttga tctattggta cattgacaga atcaattcca atcaactgga tgtttcgcag
     9781 aataaaaggc aagacagtcg cgtttagttt gattccccca gcattgccac ataaagctac
     9841 tgcaccgtta taagagatca atggaagaag actgcttagc tgctcgccac ctactgtgtc
     9901 gatgacatag tcgatttgct gttttcctaa agcttttgtt gtctctggca gaaattcatc
     9961 aggtcgtacg acttgcaaag cacctagact ttttagccat gtgacagact cttcttttct
    10021 ggaaaaggca atgattgact ggaatcctag tttatgtaac agggctatcg ctgtactgcc
    10081 tacaccgcca gaagcaccag taacgacaac ttttttatcg gaggtcatac cttgacggat
    10141 taaagcgttc acacacaaca tagcagtaaa ccctgctgta ccaaggatca tcgcttgacg
    10201 cagcgtcata ttttttggta gaggaactaa ccagtctcct ggtacttttt gatattggct
    10261 ataaccgcct ggatgggtga cgccaagccc aaaaccagta gcaagtacaa gatcttcttt
    10321 tgaaaaacgt ggattgttac tttggacgac agttccggat aaatcaatcc ctggagtcat
    10381 cggatatgtt cgtatcacct gaccagactc gctcattgcc aatgcatctt tataattgac
    10441 atcagaatag gcaattttta ccaagacttc attttcagcc aactcagtaa atggcagatc
    10501 actgattgct gtatggaaaa caggttcctt tattacctga aaagatcgaa aagcgttcat
    10561 tcgtgagaca ctccttttaa aattttcttt tatcatacca taaagcctac tcttttattc
    10621 ttgttcttcc gtaaatttag acaaaaatta ggtgaaaaat aaagaatcaa acaactagaa
    10681 tttattccaa aagataaaga aatattcagc aaaaaaaatt ttacaaaggg ttgccttttt
    10741 caataaattt cgtatactaa tacgagtggt agtaaacagg aagtttacta ttagaaaact
    10801 tcgattcgga ggaaattatg gaaattggtg agaagttaag aaatttgcgc atccaaaaga
    10861 atttgacaca agaagaacta ggcgaacgca cagatttgtc aaaagggtat atttcccagt
    10921 tggaacgaga tttaagttca ccatcaatgg aaacattctt ttctattctt gaagtcttag
    10981 gtgtgactcc agaagaattt tttcatcaag aagcagcgaa tctgcaagtg gtttattcaa
    11041 aagaagatca tacggtttat ttcgatgaag aaaacgggta tgaattggaa tggctcgtgg
    11101 ctgattcgaa tgaaaaagaa atggagcctg tcttgctgac ttttgaaggt gcaggagaat
    11161 ataagacgtt tgaaccttca ttatctgaaa cgtttatttt tgtattagaa ggggaattag
    11221 agttaagctt aggagagacg gtctatgtgg caaaagcagg acagtcgatc tattatcaag
    11281 cgacaaaaca acatcaatta aggaatcatc tcaaaaaacg aacaaaagta ttgattgttg
    11341 caacgcaatc atatttgtaa tatagggggt aaaaacgtgg ggaaaccaat catttcattt
    11401 gatcaagtag tgaaacgcta tgatgatgaa acagtattaa aaaaagtaag ttttgagatc
    11461 gaacaaggaa aattttatac actgcttggt ccttccggct gtggtaaaac aacgatccta
    11521 cgtattatag ctggattcac cgaagctaca gaaggggaca tctattttga agggaaacgg
    11581 ttgaatgatt tgccagctaa taagcgacaa gtaaatacag tcttccaaga ttatgcactt
    11641 tttcctcata tgaatgtttt tgataacgtt gcttttggat tgaagatcaa gaaaatgtcc
    11701 aaatcagata tcgaaaagaa ggtaaaagat gctttgcgta tggtccagct tcctggatac
    11761 gaaaaacggg agattagtga aatgtctggt ggtcaaagac agcgtgtagc gattgcacga
    11821 gcaattgtaa atgaaccgaa agtattgcta ttagatgaac cgttatcggc gcttgatttg
    11881 aaactacgta cagatatgca gtatgaactg cgggatctgc aacaacgatt aggaatcacg
    11941 tttatttttg ttactcatga ccaagaagaa gcgttggcaa tgagtgatga gatttttgtc
    12001 atgaataaag ggaaaatcgt ccaaagtggt acacccgtag atatttatga tgagccaatc
    12061 aatcatttcg ttgctgattt tgttggggag agcaatatcg tggacggtgt aatgatcgaa
    12121 gataatctag ttgagtttgt tggtaagcaa tttgaatgtg tcgatggcgg gatgcgtcta
    12181 aatgaaccag ttgaagttgt tttacgccca gaggatttga cgatcacgac accagataaa
    12241 ggaaaactag tcgtaacggt cgatactcag ctgtttcgtg gtgtccatta tgaaatcatc
    12301 tgttatgatg aacagcaaaa tgaatggatg gtccattcaa cgaaaaaagc taaagaagga
    12361 agcaaagtag gattggcctt tgagccagaa gatatccatg tcatgcgttt caacgagtct
    12421 gaagaagaat tcgatgcacg tcttgacagc tacgaagaat aggggggaca agaatgaaaa
    12481 caatgcgcag gatctattcc gtcccttata ttttgtggct cggtttattc gtgatcgcgc
    12541 ctgtattaat gatcatttat caatcttttt ttgatatgaa tgggcaattc acgctgaata
    12601 attatctatc ttattttacg tcggggacat cgctttctat gacgatcaat tctgtatggt
    12661 atgcattttt gatcacgttt ttcacgctga tcatcagtta cccgactgct tattttttaa
    12721 caaaactgaa gcacaagcaa ttatggctga tgctgattat tttgcctaca tgggtgaatc
    12781 tgctgcttaa agcttacgca tttattggga ttttcagtgt gcacggcagc gtgaatcatt
    12841 tctttgattt tatcgggatc gggccgaaac agatattatt cacagacttc agttttctgt
    12901 tcgtggcaac gtatatcgag attccattta tgattatgcc aattttcaat gcattggaag
    12961 agatgaatcc ttctttgatc agtgctagta gagatttagg cgcgaatagc tttgagacct
    13021 tccgacgagt gatttttccg ttatcattaa atggtgtgaa aagtggtgtc caagcagtat
    13081 ttattccttc tctttcttta ttcatgctga ctcgtctgat tggcggaaat cgggtgatca
    13141 ctttaggaac ggctatcgaa cagcatttct tggttacaca aaactggggg atgggttcta
    13201 caatcggtgt gattttaatc attgctatgt tcatcgtgat gctcctgaca ggcgagaaga
    13261 aaaaaggagg tcgctcataa atgaaaaaat ttaaatggtc gtatctttat ttagggatcg
    13321 tctttttatt cctatatgca ccgatcttct atctgatttt ttattccttc aatgaaggag
    13381 gaacgatgaa cagctttaca ggatttactt gggaaaatta tgaagctgtt tttgaagata
    13441 cacgtttgat cattatcgta ttgaatacgt tcatgctggc tttcttatct gcattgatcg
    13501 ccacgatcat cgggacattt ggggctatgg gaatctatta cacgaaaaaa cgacgtgcac
    13561 ggacagcgtt gttgagcatg aataacatct tattagtctc tcctgatgtt attatcggtg
    13621 ctagtttctt gattttcttt acactactag gattcggatt aggttttcag agtgtattgc
    13681 tttcccatat tgctttcagc attccaatcg tcgtcttgat ggtcttgcca aaattgcagg
    13741 aaatgaacga ttcaatggtc gatgcggcta gagaccttgg tgctaataat ttccaagtag
    13801 tcaaaaatat cattttgcct tatctttctc caggaatcat agctggatat tttatggcat
    13861 ttacctattc tcttgatgat tttgcggtta catttttcgt cacaggaaat ggctttacga
    13921 cgctctcagt agagatctat tctcgtgcaa gacaaggaat cagtttagaa atcaatgcgc
    13981 taagtgcgtt agttttcttg ttctccatga tattggtcat tggatactac tttattagta
    14041 aagaaaaccg tccaaaacgg ctgaagaaaa acagaagagt acgcgagggg gtggcacggc
    14101 tgcgatgaaa aaattacaat ctctgttttt aggtattctc atcattattg cggtgctgct
    14161 gcttggtgtt cgtcaacttg aaaaaacaag tggcatgagt ggagcaaagg tacttaccat
    14221 ctataactgg ggagattata tcgatcctag tttgatcacg aaatttgaaa aagaatatgg
    14281 atacaaagtg aattatgaaa catttgattc taatgaagcg atgttcacaa aaatccagca
    14341 aggtgggaca aactacgaca tcgctatacc aagtgaatat atgatccaaa aaatgatcaa
    14401 agaaaaacta gtacttccat tagatcattc gaagatcaaa ggattaaata atattgatcc
    14461 acgattttta gacttggatt ttgatccaga taatcagtat tcgattccat atttttgggg
    14521 aactttaggg atcgtatata acgataaatt ctttgatgca aatcaaatta agcattggga
    14581 tgatttgtgg aagccggaat taaaagacag tttgatgctg attgacggtg cacgagaagt
    14641 tatggggctt tctttgaata gtttaggcta ttctttgaat agtaagaata tggcacagct
    14701 gacagaagca gcaaataaat tgaatcattt aacaccaaat gtcaaagcaa tcgtggcaga
    14761 tgagatcaaa atgtatatga tcaatgaaga agcctccgtg gcagtgacat tttctggcga
    14821 agctgccgat atgatgtggg aaaatgaaca tttacattat gtgatcccac cagaaggatc
    14881 caatctttgg tttgataata tcgttatgcc aaaaaccgtg aaaaataaag aaggtgccta
    14941 tgatttcatc aactttatgc tagaaccgga aaatgcggca caaaatgcag agtatattgg
    15001 ttattctaca ccaaatgaag aagcaaaagc gttattgcca aaagacattt cgtcggacga
    15061 acaattctat ccatctgatg acacgatctc ccatcttgaa gtctacaaag atctaggatc
    15121 gaaataccta ggcatctata atgacctatt cttagaattc aagatgtata ggagataatg
    15181 gcaaaactca gtaagaatgg ggacttatca acgttctaaa gagctaaaaa tcgtcctttt
    15241 tctatctgag ttttccaatc ttctaccaaa agcggatgaa acgccgcttt ttttgtgtta
    15301 ttttattttt tctaccacgt ttttctacca gagaaaagtg gacaaaacca ttgtaaaata
    15361 gggcccgaga cgggctagta aatctaagat tgaaaaagtt acagaaggat aaagtaattg
    15421 gaaactatgc aaatatactt aacccttctg taatctatta ataaaactaa tatatgaatc
    15481 cagttcaata tttgtagcta tgaaaaaaca taaaaatcat attagataaa aactaaattg
    15541 gtagataaat tattttgtca gatatctgag gcaaatattc gactatttgt gtttcaaagt
    15601 aaaaattgga atacaaatat agaatagaca tattgcagcc gatagtttat tatgctttta
    15661 gacacattaa actggaggaa aaaatgaaaa agagcgctaa agatcgagtt tttgatgagc
    15721 tgatacgact aacagggaat atgtcttctg tcactgccga gaaaatttcg aagaatctag
    15781 gtatctctcg tcagaatgcc agccattatt tgacgagatt agtggaagat aaaaaggtcg
    15841 agaaattacc gggaaagcct gttttgtgga agcctttaga tgaatatgcg gttgtagata
    15901 atacggagca aataaacgaa gcctttcatt ctgtagttgg tcatgacgga agtctgagag
    15961 aggtcattca aaaatgtata gccgctgtca aatatccgcc taacggactc agtgtattaa
    16021 taaatggagc cacgggtgtt ggaaaaagtt ttttagccac aaaaattttt gaatacgcta
    16081 tccatgagca aattattgaa aaagatgcac catttgctat cttaaactgt gccgattatg
    16141 cggacaatcc cgaactgctt tcggcaacac tgtttggata caagaagggg gcctttacgg
    16201 gggctgaaaa ggataccgat ggtttattgg caacggccaa caacggctat ctttttctcg
    16261 atgaggtcca tcgcctatcg aaagaaaatc aagaaaagct ctttctattt attgatactg
    16321 gaaactatcg tccagtcggt gaaaatgtaa attggcattc tgctaaggtt cgttttattt
    16381 tcgccacgac ggaaaaaggt gaaaattatt tattagacac ctttgatcgg cgtattcaaa
    16441 ttagtgttat gttgccaact tttgaagatc gccccattcg agaacgatta gagctgattc
    16501 aattattttt tcaaaatgaa gcggatgttt tacaaaaaga tattattgtt tcaagagaag
    16561 ccttgtcttt aatgttatct catcccttta gtgggaatat tggtaagctg aagaatatca
    16621 ttaaaatcag ttgtgcggat gtgtatagtc gaacacaaga agaaccatta acgattggga
    16681 aaaatgagat cttgattcag ttaaatatgc tggaatccga agtagaaagc ttgccgattg
    16741 ctagtttatt gataaagaaa aatcaaaaga taaaaacgga tcgaatcaaa ccgtttgatt
    16801 atgattcgat tttggaaaaa attactcagc tgataaatga aggtaatttc aatgaaaatt
    16861 tttcttcgat aaagaatgaa gtacagcatt tggtgcatca cattgataag gattcttttt
    16921 tatcaatgag tagaaaattg atgattcaga tgtttagaaa ggtctgggta tccgttttag
    16981 gaaaaacgta cggattgacg acgagtggac ctgttgcaga aattattagc agatcctatg
    17041 tcatttcccc aagctataag acagacttaa ccgggataaa tcgtacatta gctgcaaatc
    17101 tacctcggac gacttactta tgtaatcagt tcttggagca tttaccaccg ctctcagaaa
    17161 aggacgagaa ctttctaaga ctgcttattg tcgtgacttt atctgattat gtagatgaat
    17221 ccatcgagct gaagggcctg ttgcttgctc acggagatag tacggctagc agcattcaag
    17281 cagtagtgaa tcaattatgc ggcaattatg tttttgaagc gttggatatg ccagtagaga
    17341 gtaatgtgtc agagattgtc gaatcgacaa ggaatttcgt gaaacggcaa gtgaatacag
    17401 atagtctagt gctgatcgta gatatgggct ctttaaatca aatttacaca cagatcaaga
    17461 acgatttaaa aggggagtta cttcttctga ataatttgac gacttccatt gcacttgata
    17521 ttggcttgaa gatgacgatg aatgcgccat ttaaatagat tgcggaggat gcagcagaca
    17581 agtattcaat caatgttcaa tactttgaag gcttttctca aagtagcaac attatcattt
    17641 cctgtatgtc gggtttgggg atttcggaca aattgaaaga tgtttttcgt catcatttat
    17701 cggatgaatc catcgaagtt ttcacgaagg actacagaga actacgtgaa ttaatcgacc
    17761 aaaatgatga aacttatttt gacaagacaa aattggtcat cacgacttcg gatcttccac
    17821 gttccttttc aattcctaat attaatgttt atgacatctt agacggtgat ggagcagcca
    17881 atttggagca tattttgagt cctgaaatga agccggcaga atttaaccgc ttgattcagg
    17941 agctagtaaa attcttttcg ttggaaggaa ttgcggaccg tttgaacttt ctgaacccaa
    18001 cgatcgtgat gagtgaagtg gaaacagtca tttctaaata tgaaaattac tatcatttca
    18061 ctttaaatgg ccgtgtaaaa ctaaatctct atatgcatac ggcattgatg atggaacgtt
    18121 tattcttggc tcggacagaa aaatctgaag aaccgaatta tgatttgaaa ccagatgaag
    18181 atgaatttta ttatgtttct agaagtattt ttcaatcaat ggagatgaaa tataatttta
    18241 aagtcaatgc ttatgagctt tcgctgctct atgaattatt aggaccgtat attcaaaaat
    18301 aaagtgtcta aaaaagtgtc taatctgttt tttttaggtt agacactttt tttaatgtga
    18361 attaacgtga ttaaaccaat gtttttaaaa gttggcacag gaattgctta tataaaagtg
    18421 aacagaaaga aaggaattaa ccatatgaag aaaattctaa tagcgactca cggtcactta
    18481 gcttcaggct ttcttagcag tattcaatta ctgacgggga aggttcaaga gattacggtg
    18541 attaatgctt atgtggatga gtgtgatttt gaacaggagt tgaatgtatt tgtaaaccag
    18601 gtgaacgagc aggatcagat ttttgttttt accgatttat ttggcggaag tgtcaatcag
    18661 aagattacca aaaccttttt ggagaaagag attgctgtaa ctattttgtc tggctttaat
    18721 ctgccaattc tgctcgaaat agtattggca accaaggaat taacaaagga ggatattcag
    18781 gaactggtaa ataaatgcca acaggaatta aagatgacca ttcttgatga ggtacatgta
    18841 gtaaatgaag aggaagattt tttttaggag gaagaaaaat gattaaagca gtgcgaattg
    18901 atgaacgatt aattcatgga caagtagcga tgacttggac aaagagtcta aatttaaccg
    18961 ggttagtagt agcaagcgat gaggcggcta ccaacgatat tcaaaagatg acattgaaga
    19021 tggctgcacc atcgaatgtc aaagtcatta ttaaaacagt tgatgacgcg attcatttgt
    19081 taaaagatcc gcgggcgaag gacatgcgct tgatggtttt agtccctaat gtcaaagatg
    19141 cggtaaaagt tgcgaaagct ttcccaacgg aaatcgaatt aatgaacgtg gggaatgctg
    19201 ggaaaatgac accgccatcg gaagataaga aaaccttatc aaaggaagtg atgttaacga
    19261 cgtcagaaat tcaagactta aaagaattag ttgaacttta tccagatacc gtttttcagc
    19321 ctacaccggc gatggaaaaa cgttcagcta tctctatttt gagtgatttt tagaaaaagg
    19381 aaggagaaaa attatgtatg atgcatttgt agtcgctttg gccgttttta ttggagttgc
    19441 tggacatgaa atgttcggga tgtccatgct gagtcgtccg attgttgttg ctccgttagc
    19501 aggagcgttg atgggggatt tgcagacggg gttgattgtc ggggcgtcac tagaagccat
    19561 ctttatgggt gtcgtcaata tcggaatctc tagtacggcg gagccttctt tggctgctgg
    19621 gctggctaca gcgttcgcaa tcaaaatggg aggaaacgtg gatgcgatta ttcccattgt
    19681 ctttccacta gcagtattag gattacagtt aatgaatatg attttttctt ttgtatgtgg
    19741 accgatggct gctaaatttt tcaaatttgc aaaatctggt gatcaaaaag gaatcatcaa
    19801 tctgcacttc tttatgtggt ttgtccacta tgcgctttat gctcttattc cattctttgc
    19861 cgttttattt ggagcagatg ttgtcaaaag cgtcttggat aatattccag aggtcatcat
    19921 gaacggatta acagtcgctg gaaatctgct gccggcagtt gggatggcga tgctgttacg
    19981 gatgctttgg agtaaaaata ttgctgtgtg gtttttcttt ggggccgttg ttatggcgta
    20041 tctcgagtta ccattgattg caattgccgc agttggaacg attattgcaa ttgttatagc
    20101 ccaaagagat ctacaactaa aaaatatatc tgttcatgca gttcaaagcg atgctgctgt
    20161 aaatgcaacg cttgaccaag aagaggagga tttctttcaa tgaaattagc tgatggaatt
    20221 tctaaagatg agaaaaaatt ggttcggaag atgttttggc gctcgatgac tatgtatgct
    20281 tcggtgaatc cggtgacgat gggcggcggt ggtttcgcct attctatgat gcccttcatt
    20341 aatcatttct acaaaagtga agaagatcga gctgcggcgc tggaacgtca tacacagtat
    20401 ttttcgacga ccattccttg cgcgtccttt gtcatgggga ttgcggcatc gatggagaag
    20461 gaaaattctg aaaaagctgg ttttgacgtg gaaagtatca acgcgatcaa aacgagtttg
    20521 atgggaccat tggctggaat tggcgattca ttgttctggg gagtttggcg tgtcgttacc
    20581 gcgggactag cgatcaactt agccaatgcc ggaaatattt tggcaccaat tatcttttta
    20641 gttatgttca atatacctaa ttacttgatg cgatactatg gcggcgtttt aggctattca
    20701 ttaggctcta aatatattga aaagctgtat gcgaacgggt taattgaaat tctgacaaaa
    20761 gctgctagta tcttgggtct gatcatggta ggtgcaatga cgatcagtat ggtcaaattc
    20821 aataccgttt tgagcttttc aatgaagggc gaagaactaa tgaatgtcca agaaatcctc
    20881 gatagtattt ttaaaggaat tgtaccgtta ttggtaacat tgagctgttt caaattacta
    20941 gataaaaaag tcggcgtcat caaaattatt tttggaatgg tcgtcttggg aattttattg
    21001 tcattacttg gaatcgcata gtaggaagct gcagctacta ggctgcagtt tttttagcaa
    21061 ggagggaaaa gatgactatt aatgaagtaa tttattcacc caaacaccca ttttttaatg
    21121 ctgagttaaa cgacggaaat gtgactactt caaacgaata cagctactat cggaatattc
    21181 ctgatgtaaa gggattgacc ttcgatgtaa gtaatcaaaa gacattagca aaccttgatg
    21241 tttgcgggac gattcaaacg ttgacctttt atcgagaaaa tttactaact gaggaaaaac
    21301 caggtgtttg ggtcaataaa cagctctctc aaaccaattc gttatcctta aaggttgaag
    21361 taaacgggat tgtctaccat ttgtcagaat cggatcatcg tattgaagtc gatgtgattc
    21421 aagactgttt gcctagatat attcatcatt attcgacgtt taaggtcatt gtagtatcct
    21481 ttgctcccat actggctaat aagcgtttaa gtatgcttgt tcaacaggtt tacattgtga
    21541 atgaaacaca cgaaagcctt caagtttcag tgaaagatgt gccttcgtat caagagaagt
    21601 attcggatca gcaaaatgtg ttgatttctc aaaaaggaaa taaggacacg atcgcacaac
    21661 aggaagtggc aagcttcgcc attgcttttg tggacccaaa tgcttttgaa gaagtaatga
    21721 cttttcagga atctgatatc gaaaaatggc tgaaggaaac attgaactat tttgctcagc
    21781 tatttgggca gctgtcaatg ccaaacgata gagttgtcca tctctatcgt cgtgcacttt
    21841 atcaaagctt ttctagtttc ggtatgaatc gacagggaca ggtcgtcggc agcaattggg
    21901 gcagctatcc tgctactaat cgaatttgga ataaggatat gtattacgca tcattgccat
    21961 tcaccatgtt tgaaccggag ttatgtcaaa aaacggttct atggtttgat caatatggcg
    22021 ttaagtttcc tggttcaaaa tttacaggag gtatcaatca ttctctgagt aattcgttat
    22081 ccagtgcgtt actggctagt ctttattttg aacatactgg ggatacaaaa ttctttgagc
    22141 ggtgtccgca ggttctactg aatgcttcaa gaattattga ggacatatta gctcaacgtc
    22201 ggtcagggga accaatgctt tttccatctg tttggttatc agacgccttt gcacttggaa
    22261 agtatcatac cggcagcaat ctttgtcttt ggaaggcctg tattggactt gcagaaattt
    22321 ttgaagccct agaacaactg agtcttagca agcgatacaa aaacattgcc tgtaaactaa
    22381 aggaatcaat ttgtaaacaa atgactatcg atggtccttt tggtgagcaa tttttggagg
    22441 gaatcggtga tgaggagaaa acaacctatt cagtgaagca ctatcaaaaa ccgattcttg
    22501 agcaagggtt aatcttttta agtgatgtga ttgtggatgg aaaaattaat ttattgatgc
    22561 atgatggaga agaatcagac actacgttga tgccttttta tcaattttta gataataaag
    22621 atcatttata tcagaacacc atggcctttt ctgctagttc tttcaatcca acctatagcg
    22681 aggaaatcaa aggcatcact tggggattag aatccggcgc cacttttcct ggctttatca
    22741 ctgttttaat gagcgagctg cacaaccctc aagctttcgt aacccgatta gaagaactaa
    22801 tttgtttggc agatttagac ggttcgtggt ggtggtggcc gtatcgctta gaggctcaac
    22861 aaggagaagt cgtgcgagac tttggctgcg gcaaatgcgg ctgggcatcg ggtctttttg
    22921 tcagcctaat gatcagtcaa tatttcggat tgaaattcaa aaaaggagta ttgcaaattg
    22981 atcctgttga taatttaacg ttttcttgga agaatttgaa gttcggtcaa gccttcattt
    23041 cgattgaatg tacgcaatcc gagttgagta tttcaaattt gtcagaaaag ccattgaaaa
    23101 taagtgatac gaaaaaaatt cttcacgtag aaaagggaaa aacgattcac attcaaagga
    23161 gaaaaagatg agtatactaa aaaatattac ccaatttaaa aaaatccaga gtcgagcaat
    23221 cactcccgaa aatccaacag gcgagaaagg taaagctgcg atgttgccaa gcgagctagg
    23281 accaagccgc aaaggtcgtg gcgcaattcc gctgacttct ggtgaagaaa ctgtgattgc
    23341 ggatattact ggaacgggaa taattcgtca tatgtggatg acgattcgtg atcgaacaga
    23401 atttggcagt tttgttttac gtgatttaat cttaagaatt tattgggatg ggtcggatag
    23461 ccctgctgtt gaaagtcctt taggggattt cttttgtaat gggtttggag agcgttgcga
    23521 ggtgaactcg ttgccgatag tggttaatcc tacagggggc tttaattcct atttcgaaat
    23581 gcctttccaa acatccgcta aaattacgat cgagaattta catccgaagg atatgtcctc
    23641 atttttttat acgataaatt acgctttggt ggacgaattg gaagaaaata gcctatattt
    23701 tcacgcaaat tggcatcggg aacgcattac caaattacag gaggattata agttaattga
    23761 tcgattaaag ggtcaaggct attacgtggg tacttacctt gcgcttacct gcctagaacg
    23821 ttattggtgg ggcgaaggcg agtttaaatt ctacattgat ggggatagtg agtatccgac
    23881 cgtaacctca acaggttcag aagattattt tggcggggcg tgggcctttc atgagtacga
    23941 tgagttcgga cgtccacatg cgaaaaacta ttcaacagcc tttttagggt atccttacca
    24001 atcaaataga gatgccacaa gagatcgttt tgaaacggga aaattgaatg ccgttcatgc
    24061 ctttggtgac gacgcaatgc cacgacatgg tctttatcgt tggcatcttc aagaccccat
    24121 ctgttttacc gaagatttat cggtcacttt acaacaaatc ggcaacgatg atgttcgctt
    24181 atttgagcgg caagacgatg ttgctagtgt cgcttactgg tattcaacag aagcttcagg
    24241 aaatactaaa cgtcatttca ctgatcgaga acgcttgccg cgttaatgaa gtgtttttca
    24301 catattcgaa gggaggaacc tatgatgata tatggggcaa ttgaagctgg agggactaaa
    24361 tttgtctgtg cagtcggtac tgaggaatta acgattttgg aaagagtcag ttttcccaca
    24421 acaactccag ttgaaacaat gaagcaggtc attgattttt ttaaaccttt taaggaacaa
    24481 ttggcaggca tcggggtcgg ctgctttggt ccaattgata ttcaatgcaa atcaacgagt
    24541 ttcggtcata tcacatcaac accaaaactt gcttggcaaa atttcaattt tgtaggaacg
    24601 ctaaagcaat atttcaatat tccgattgaa tggacaacag atgtcaatgc ggcttgctat
    24661 ggtgaatatg ttgctggttc tggcaaaggg ttagcaggtg ttgcatactt cactgtggga
    24721 acaggtatcg gaggtggtgc cttgttcaat gggactttcg tggaagggtt cagtcatccg
    24781 gaaatggggc atatgttgat caagcgacat ccaaaggaca atttttcagg aaactgtccc
    24841 ttccaccaag attgtttaga ggggatggca gcaggaccag cgattgaaaa acggttgggt
    24901 gtaaaaggcc agaatttatt ggctgacgat tcgttttggc agattgaagc cttttacctt
    24961 gcccaatgtg catacaatac aacactgatg ttttcgccag atagaattat ttttggcggt
    25021 ggtgtaatga aacaagaaca catgaagaaa aaagttcagg ataaatttgt tgagcttata
    25081 aatggatatg ttgaaatacc accaattgat agctacatta ttacgcccga attaggtgat
    25141 aatgccggaa taatcggcgg attagcattg gccagaaaag cggtaagaaa taagcaacca
    25201 taattaatga atgtttcatt gatactcatg tcaaaagtat ttaaccgagt aatcaaatga
    25261 tcaaatgaag ttggataaag cgaaaatgaa ttcaaataaa aaggctctaa gtcaaatcgt
    25321 gttgttttca ttaaatcact gaaaggaaat gcttctgcgt ttcctttttt ctttttgttg
    25381 tgcgctgtat accttgagga tattcactgg tggcgtacac ttaaatagta atatattgtg
    25441 ttactttatg tctgtggtaa tagttatagt tgcttaccaa tgtatgttca tttttcgaaa
    25501 tgttgacttc ataatccttg gcttaatgtt tgaatttttt tggtgata
//
LOCUS       DAZAFF010000034        25348 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000034 DAZAFF010000000
VERSION     DAZAFF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.034"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..499)
                     /locus_tag="ACG9BB_001347"
     CDS             complement(122..499)
                     /locus_tag="ACG9BB_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837294.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            755..1483
                     /gene="purC"
                     /locus_tag="ACG9BB_001348"
     CDS             755..1483
                     /gene="purC"
                     /locus_tag="ACG9BB_001348"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFN2837295.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1483..1737
                     /gene="purS"
                     /locus_tag="ACG9BB_001349"
     CDS             1483..1737
                     /gene="purS"
                     /locus_tag="ACG9BB_001349"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFN2837296.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1739..2410
                     /gene="purQ"
                     /locus_tag="ACG9BB_001350"
     CDS             1739..2410
                     /gene="purQ"
                     /locus_tag="ACG9BB_001350"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFN2837297.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2411..4633
                     /gene="purL"
                     /locus_tag="ACG9BB_001351"
     CDS             2411..4633
                     /gene="purL"
                     /locus_tag="ACG9BB_001351"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFN2837298.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4618..6057
                     /gene="purF"
                     /locus_tag="ACG9BB_001352"
     CDS             4618..6057
                     /gene="purF"
                     /locus_tag="ACG9BB_001352"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFN2837299.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6080..7132
                     /gene="purM"
                     /locus_tag="ACG9BB_001353"
     CDS             6080..7132
                     /gene="purM"
                     /locus_tag="ACG9BB_001353"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFN2837300.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7129..7707
                     /gene="purN"
                     /locus_tag="ACG9BB_001354"
     CDS             7129..7707
                     /gene="purN"
                     /locus_tag="ACG9BB_001354"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFN2837301.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7704..9239
                     /gene="purH"
                     /locus_tag="ACG9BB_001355"
     CDS             7704..9239
                     /gene="purH"
                     /locus_tag="ACG9BB_001355"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFN2837302.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9255..10529
                     /gene="purD"
                     /locus_tag="ACG9BB_001356"
     CDS             9255..10529
                     /gene="purD"
                     /locus_tag="ACG9BB_001356"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFN2837303.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10651..11565
                     /locus_tag="ACG9BB_001357"
     CDS             10651..11565
                     /locus_tag="ACG9BB_001357"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFN2837304.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11698..13263)
                     /locus_tag="ACG9BB_001358"
     CDS             complement(11698..13263)
                     /locus_tag="ACG9BB_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2837305.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13256..14707)
                     /locus_tag="ACG9BB_001359"
     CDS             complement(13256..14707)
                     /locus_tag="ACG9BB_001359"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN2837306.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14721..15737)
                     /locus_tag="ACG9BB_001360"
     CDS             complement(14721..15737)
                     /locus_tag="ACG9BB_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2837307.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15957..17045
                     /gene="chvE"
                     /locus_tag="ACG9BB_001361"
     CDS             15957..17045
                     /gene="chvE"
                     /locus_tag="ACG9BB_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN2837308.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17099..18625
                     /gene="mmsA"
                     /locus_tag="ACG9BB_001362"
     CDS             17099..18625
                     /gene="mmsA"
                     /locus_tag="ACG9BB_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN2837309.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18627..19832
                     /gene="mmsB"
                     /locus_tag="ACG9BB_001363"
     CDS             18627..19832
                     /gene="mmsB"
                     /locus_tag="ACG9BB_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFN2837310.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20089..21066
                     /gene="guaC"
                     /locus_tag="ACG9BB_001364"
     CDS             20089..21066
                     /gene="guaC"
                     /locus_tag="ACG9BB_001364"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFN2837311.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21330..22754
                     /locus_tag="ACG9BB_001365"
     CDS             21330..22754
                     /locus_tag="ACG9BB_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFN2837312.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22796..23053)
                     /locus_tag="ACG9BB_001366"
     CDS             complement(22796..23053)
                     /locus_tag="ACG9BB_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837313.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23215..24324
                     /gene="pdhA"
                     /locus_tag="ACG9BB_001367"
     CDS             23215..24324
                     /gene="pdhA"
                     /locus_tag="ACG9BB_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFN2837314.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24327..25304
                     /locus_tag="ACG9BB_001368"
     CDS             24327..25304
                     /locus_tag="ACG9BB_001368"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFN2837315.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8194 a         4316 c         5694 g         7144 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
      661 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
      721 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
      781 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
      841 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
      901 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
      961 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     1021 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     1081 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     1141 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
     1201 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     1261 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     1321 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     1381 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     1441 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     1501 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     1561 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     1621 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     1681 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     1741 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     1801 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     1861 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     1921 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     1981 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     2041 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     2101 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     2161 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     2221 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     2281 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     2341 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     2401 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     2461 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     2521 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     2581 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     2641 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     2701 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     2761 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     2821 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     2881 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     2941 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     3001 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     3061 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     3121 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     3181 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     3241 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     3301 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     3361 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     3421 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     3481 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     3541 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     3601 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     3661 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     3721 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     3781 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     3841 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     3901 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     3961 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     4021 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     4081 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     4141 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     4201 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     4261 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     4321 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     4381 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     4441 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     4501 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     4561 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     4621 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
     4681 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
     4741 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
     4801 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
     4861 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
     4921 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
     4981 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
     5041 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
     5101 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
     5161 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
     5221 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
     5281 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
     5341 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
     5401 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
     5461 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
     5521 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
     5581 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
     5641 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
     5701 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
     5761 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
     5821 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
     5881 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
     5941 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
     6001 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
     6061 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
     6121 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
     6181 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
     6241 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
     6301 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
     6361 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
     6421 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
     6481 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
     6541 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
     6601 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
     6661 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
     6721 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
     6781 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
     6841 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
     6901 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
     6961 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc aagaatcgaa
     7021 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
     7081 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
     7141 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
     7201 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
     7261 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
     7321 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
     7381 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
     7441 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
     7501 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
     7561 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
     7621 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
     7681 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
     7741 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
     7801 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
     7861 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
     7921 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
     7981 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
     8041 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
     8101 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
     8161 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
     8221 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
     8281 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
     8341 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
     8401 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
     8461 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
     8521 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
     8581 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
     8641 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
     8701 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
     8761 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
     8821 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
     8881 agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc aaacatgtga
     8941 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
     9001 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
     9061 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
     9121 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
     9181 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
     9241 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
     9301 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
     9361 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
     9421 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
     9481 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
     9541 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
     9601 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
     9661 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
     9721 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
     9781 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
     9841 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
     9901 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
     9961 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    10021 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    10081 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    10141 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    10201 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    10261 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    10321 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    10381 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    10441 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    10501 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    10561 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    10621 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    10681 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    10741 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    10801 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    10861 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    10921 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    10981 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    11041 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    11101 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    11161 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    11221 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    11281 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    11341 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    11401 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    11461 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    11521 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    11581 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    11641 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    11701 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    11761 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    11821 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    11881 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    11941 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    12001 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    12061 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    12121 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    12181 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    12241 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    12301 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    12361 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    12421 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    12481 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    12541 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    12601 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    12661 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    12721 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    12781 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    12841 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    12901 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    12961 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    13021 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    13081 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    13141 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    13201 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    13261 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    13321 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    13381 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    13441 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    13501 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    13561 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    13621 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    13681 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    13741 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    13801 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    13861 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    13921 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    13981 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    14041 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    14101 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    14161 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    14221 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    14281 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    14341 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    14401 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    14461 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    14521 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    14581 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    14641 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    14701 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    14761 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    14821 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    14881 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    14941 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    15001 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    15061 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    15121 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    15181 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    15241 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    15301 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    15361 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    15421 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    15481 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    15541 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    15601 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    15661 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    15721 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    15781 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    15841 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    15901 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    15961 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    16021 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    16081 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    16141 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    16201 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    16261 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    16321 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    16381 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    16441 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    16501 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    16561 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    16621 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    16681 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    16741 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    16801 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    16861 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    16921 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    16981 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    17041 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    17101 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    17161 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    17221 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    17281 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    17341 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    17401 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    17461 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    17521 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    17581 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    17641 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    17701 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    17761 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    17821 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    17881 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    17941 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    18001 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    18061 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    18121 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    18181 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    18241 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    18301 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    18361 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    18421 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    18481 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    18541 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    18601 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    18661 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    18721 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    18781 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    18841 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    18901 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    18961 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    19021 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    19081 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    19141 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    19201 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    19261 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    19321 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    19381 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    19441 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    19501 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    19561 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    19621 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    19681 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    19741 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    19801 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    19861 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    19921 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    19981 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    20041 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    20101 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    20161 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    20221 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    20281 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    20341 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    20401 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    20461 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    20521 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    20581 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    20641 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    20701 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    20761 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    20821 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    20881 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    20941 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    21001 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    21061 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    21121 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    21181 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    21241 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    21301 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    21361 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    21421 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    21481 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    21541 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    21601 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    21661 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    21721 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    21781 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    21841 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    21901 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    21961 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    22021 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    22081 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    22141 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    22201 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    22261 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    22321 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    22381 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    22441 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    22501 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    22561 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    22621 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    22681 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    22741 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    22801 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    22861 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    22921 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    22981 atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    23041 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    23101 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    23161 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    23221 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    23281 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    23341 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    23401 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    23461 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    23521 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    23581 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    23641 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    23701 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    23761 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    23821 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    23881 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    23941 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    24001 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    24061 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    24121 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    24181 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    24241 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    24301 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    24361 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    24421 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    24481 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    24541 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    24601 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    24661 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    24721 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    24781 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    24841 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    24901 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    24961 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    25021 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    25081 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    25141 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    25201 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    25261 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    25321 acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DAZAFF010000035        24763 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000035 DAZAFF010000000
VERSION     DAZAFF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.035"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            243..1384
                     /locus_tag="ACG9BB_001369"
                     /pseudo
     CDS             243..1384
                     /locus_tag="ACG9BB_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1510..2589)
                     /locus_tag="ACG9BB_001370"
     CDS             complement(1510..2589)
                     /locus_tag="ACG9BB_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN2837316.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2692..3132)
                     /locus_tag="ACG9BB_001371"
     CDS             complement(2692..3132)
                     /locus_tag="ACG9BB_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN2837317.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3189..3599)
                     /locus_tag="ACG9BB_001372"
     CDS             complement(3189..3599)
                     /locus_tag="ACG9BB_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN2837318.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3779..4213)
                     /locus_tag="ACG9BB_001373"
     CDS             complement(3779..4213)
                     /locus_tag="ACG9BB_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN2837319.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4313..5161)
                     /locus_tag="ACG9BB_001374"
     CDS             complement(4313..5161)
                     /locus_tag="ACG9BB_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN2837320.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5550..7316
                     /locus_tag="ACG9BB_001375"
     CDS             5550..7316
                     /locus_tag="ACG9BB_001375"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFN2837321.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7418..8677
                     /locus_tag="ACG9BB_001376"
     CDS             7418..8677
                     /locus_tag="ACG9BB_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFN2837322.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8824..10125
                     /locus_tag="ACG9BB_001377"
     CDS             8824..10125
                     /locus_tag="ACG9BB_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2837323.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10128..10979
                     /locus_tag="ACG9BB_001378"
     CDS             10128..10979
                     /locus_tag="ACG9BB_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFN2837324.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10992..11807
                     /locus_tag="ACG9BB_001379"
     CDS             10992..11807
                     /locus_tag="ACG9BB_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFN2837325.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11958..13124
                     /locus_tag="ACG9BB_001380"
     CDS             11958..13124
                     /locus_tag="ACG9BB_001380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN2837326.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13354..14001
                     /locus_tag="ACG9BB_001381"
     CDS             13354..14001
                     /locus_tag="ACG9BB_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFN2837327.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            14085..15029
                     /locus_tag="ACG9BB_001382"
     CDS             14085..15029
                     /locus_tag="ACG9BB_001382"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFN2837328.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15164..16615
                     /locus_tag="ACG9BB_001383"
     CDS             15164..16615
                     /locus_tag="ACG9BB_001383"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFN2837329.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16636..17472
                     /locus_tag="ACG9BB_001384"
     CDS             16636..17472
                     /locus_tag="ACG9BB_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFN2837330.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17472..18132
                     /locus_tag="ACG9BB_001385"
                     /pseudo
     CDS             17472..18132
                     /locus_tag="ACG9BB_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            18258..18947
                     /locus_tag="ACG9BB_001386"
     CDS             18258..18947
                     /locus_tag="ACG9BB_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837331.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18953..19909
                     /locus_tag="ACG9BB_001387"
     CDS             18953..19909
                     /locus_tag="ACG9BB_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFN2837332.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19953..20465
                     /locus_tag="ACG9BB_001388"
     CDS             19953..20465
                     /locus_tag="ACG9BB_001388"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2837333.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20721..21269)
                     /locus_tag="ACG9BB_001389"
     CDS             complement(20721..21269)
                     /locus_tag="ACG9BB_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFN2837334.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21358..22539)
                     /locus_tag="ACG9BB_001390"
     CDS             complement(21358..22539)
                     /locus_tag="ACG9BB_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFN2837335.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(22775..24283)
                     /locus_tag="ACG9BB_001391"
     CDS             complement(22775..24283)
                     /locus_tag="ACG9BB_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFN2837336.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24442..24633
                     /locus_tag="ACG9BB_001392"
     CDS             24442..24633
                     /locus_tag="ACG9BB_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837337.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
BASE COUNT         8274 a         4491 c         4733 g         7265 t
ORIGIN      
        1 aagaaacgat gttcagatag gaaaagaaaa atatctaatt ttgtattgct ttttttcttc
       61 aagataaacg ttttaatcgc ttcttgagtt tgcatgaaag aggctccttt ttacttaaat
      121 tgttaaaact tgaaaatatt actgtttttt tcataaattt agacaaaaaa acagtaaaca
      181 tataggtaga atctctacta tcttatttct tgcatacaaa aaaatcaagg agtgagagag
      241 ttatgagata ttcaaccatc ttaatatcaa catttgcagc cttaagctta tcgcttacta
      301 taaatcagac agtccacaca gaggagacag aaacagccaa atggacagaa accaagtggg
      361 ccaacgtcaa tcgaattacc tttcaggatg aattttcatc cttcaattca ggagtcgcaa
      421 aattaagaaa aggacaaaca gcgaccatca aagcaaaaat tgactattcg ggagacttag
      481 cacctatact acaatcagct gttacatttg agaatgttga tcccactttg tcgattggct
      541 atgacgagaa cactttgact atccaacaca agcaagcgat atttaccttc accgttacat
      601 taaatcagga cttgttaaaa ccagcgcttt ttactgtcaa ggtatcagat ggactaccga
      661 acgatcatca tttaacgccg tatagtcagc ggcagacaat tgagcaagat tcagccatcg
      721 actcaggagg agatcttgtt gaagaaccaa cagataaacc agaaaatgaa aataagccag
      781 aagtaccgcc tacagaaaat ccagacggag aacagaaacc ggaaatagaa cctggagaag
      841 aacccgatac agaaactcaa ccagagccag acaataaatc aaaacctgaa ataacccctg
      901 gagaaaaacc agatgtaact ccagaaccag atacggattc tggaaaccaa acagtaccgg
      961 aaacaaatcc agacacagat aatgaaactg aaaatccaga aaaaccagag gtagatccag
     1021 aagaaaaacc agatgtgact ccagaaccag atacggacgc aagagaccaa ggaataccag
     1081 aaaaaataaa caaaaaaaca attcaagagg atggaaaaaa agagagtaaa aaatcaaatc
     1141 tagcaattct aaaaataaat gaagagcagc tgaataaaaa atcacgaata tttgatagcg
     1201 cccaatcagc tgaaacctta aaaagtagca aggatacaac ctttgctgtc ctgaaacgaa
     1261 aaataaacag ctaccaaaat ccggagagtc acaaaacaaa gttatactgt ggagcggaat
     1321 cattctatta agtatagcaa caatgctttc tgcaaaaaga tttaaacaaa acagatctct
     1381 ctaacatttg aataatgtct gtgaacgcat ttttcaaata aaaagaaata tccagaaaat
     1441 gaccccaaaa taccagattg aaatctgata tttggggtcg ttcatacttt atctttcttc
     1501 atatttattt taagggatat ctgtttcttt tggaagatgg ttcgggatct ttttcacttt
     1561 caatacatct aaaaagaagg taaagatcgg aatgccgaca atcaagcccc aaacgccaaa
     1621 gaaccgttcg ctaacaagta agaccacaaa tgtataaaaa atcggcagtt ctgttttgct
     1681 ggacatcaac ttcggattca gcacataaga ctcaattaga tggacaatca aaatcgtaat
     1741 caggatataa acaacatctc ggatgcctcc ttgggaataa gctatgaaag atagaggaat
     1801 acaagaaatg ataacacctg ctactgggat cagacttaaa atgaaaatca tgatcgctaa
     1861 actcagcagc tgatgaaagc caaatacagc caaggcgatt gtagtgagca acgtatttaa
     1921 aagagcgaca acaaattgag cttctaatac caaaccaaat gtattgacaa acttatcggc
     1981 aaaatagtaa atatcttgaa agaaccaaga aaattcactt tttaaaaaca atcttgagaa
     2041 tacaattgtt tttttctttt caatcataaa aaagaaactt aagatgaatg acatagcaaa
     2101 cgccactgcc atttttccta tatcctgtaa atagccaagc gcaatcgaag cgccattttg
     2161 aagctgttca aaaaaatcgt ttcgctcaat ataagtatgg atatattgta aaacaggatc
     2221 tgcatctttt ggctgatgct cgtaaaaatt aataacggac tggatcatgt caaatgtttg
     2281 atgaataagc atcgggatat actttgtgaa tgccagatag ataaggtaaa cgatcagtgt
     2341 ataaatcatg atagctaaaa cacctgtagg gattcggaca aatcgctgaa tgatttgaac
     2401 aagatgaatc atcaaataag taaagataaa tgtgagcaaa atggttgtaa tcatacttct
     2461 aactaagaaa agaacaaaaa cgacaaataa taaaacagtg agacggcgta ttttttcatt
     2521 cgccaaaaaa cgctgataaa cggtcaaatc gacaacctcc aaataacaaa aaataaacac
     2581 gttattcatt atatgatgga atatgaaaat aagaaagagc aaaaagaaaa accgctaaat
     2641 gacgttatct tatgagttgg ttttcctcat aaaatcgttc atctagcagt ttcagccctt
     2701 tttctggtct tgatgaaaat tatctagcat ttttcgggtc agatgttgca gtgtatccaa
     2761 ttcttcctgt tcaagtggaa gataagactt gatcttttgt ggaatacctt ctgcttgttt
     2821 ttgcaacgca gcaccttgag cagtcagcgt aacatttacg attctttcat cacccgtttc
     2881 acgctgacga ttaatataat ttttttctgc taactttttt agtaagggag tcagcgtacc
     2941 agaatccaga cacaaatcat ttcctagctc tttcactgaa gtctttttct tttcccataa
     3001 aactaacatc acaagatact gggtataggt aagattcagc ggcttgagga ggggagtgta
     3061 gtgtctaatg atttctttag aggcggcata taaaggaaaa catacttgat taactaaata
     3121 tagatcttcc atttttccac ctccgctgtt ttactatagt gtatcatagc ggatttacac
     3181 aaaaaagtct attcactaac tgctttcaga gtaacttcta tatttccagc tgtagctttt
     3241 gaatacggac acactttatg cgctgcctct aataattctt gcgtttcttc cagagaaaga
     3301 ccttcgatag atccttcaat ctctacacct aagacaaagc tcgtttcact ttctgaataa
     3361 agggaaactt gtgcactgac agtagaagcc ccagagatat ttttttgctg tttgatcaat
     3421 tctaatgcac tgttaaaaca tgcactgaaa ccagctgcaa acaattgttc agggttggtt
     3481 gcattttcga cgcgtttacc aggagctgca atattcaatt cgaatgtacg atcgggatta
     3541 tgcacttctc cagagcgtcc gcctgtgttg atcattttcg tagaatacat ttttttcata
     3601 aagttaccac ctttaattat attttataca attgaatttt acacaatata attgtggaaa
     3661 gcaaagaaat ggattggcat aagataaata agagaaagca catgaaacag acgcgctttc
     3721 tcgaatagca aacatataca aaaaatatca gagacaaaaa ataatctcat cataaacatt
     3781 acatatattt ctcaaaaagt tgttttcttt cttcaaaaat tacttcacga tggacctgat
     3841 gaagcatcca taaataacca aaaggatctc tgaacattga gttagtcaca ccaaagtcca
     3901 tcatttctgt catttcttga atctctacac agccatttcg taatgcttta ttatgtgtgt
     3961 cctcgatatc agagacgatg atattgaacc agatcgtatt tggatgttca gcagtaggag
     4021 cagtcaaacc aagagacgtg ttttcatcca gcatatggaa tctcgtacca tacaaggtaa
     4081 aaatgacttc attattgcct ctagggtaat ctgttgcctc aatcagttgt atttcatcaa
     4141 agattgtttg atacagttct agtgctttca aactatcatt taatacaaaa tcgatttccg
     4201 ttcctttatg cataaagatc tcctctcctt ttcagtcgaa cagttactag attcttttat
     4261 tataccagct aaagaaaacg cattcaaaaa aatagcggat ttacttacca actcattcga
     4321 ttggatgata agtaaatccg cgtcccatta cttctgctac gctgctgaca atgacaaagg
     4381 cacttggatc gatttcttct atcaatggtt tcattcggga aaattcatga tcagtcacaa
     4441 cgatcaataa aacctctttt gactttcgat tatacccgcc ttttgcctct agcagggtaa
     4501 ggccacgtcc aatctcagca cttaagcgaa ggcggatgtc ttccaaataa tgttcactca
     4561 tgaccataac agatttcttt cgattgatac ctgtttcgat ataagtcatg acaattgaag
     4621 tgattgcaat agataaaata gcataaagaa actcttctat tccaaaaata aacagattaa
     4681 gagaaacaac gaatgcgtct gtaaaaaata agccaatcga agtattcaag tggaaatatt
     4741 ttttaaaaat gaggggaggg atcgtcgtac cgccgctaga agagttgttt ttatataaaa
     4801 tcgcaactcc taaagcaaaa atcgcacttc caaaaatgac agacaacaac cgatctgaag
     4861 taatcattgt ttctggaact attgcaatga ttaaaggaaa aaccaagctt ccgaaaagta
     4921 ctttaaaaaa gggtttttta cctaaaaata gaagagctag taccaacata acgatattca
     4981 acactaaaat aactatagct cggtcaaagc ctaaagcagc ttccagtaat attcctagac
     5041 cgctgacacc tccagcagca atatgatgag gccctaaaaa catattgata gcgactccta
     5101 atatgaagag ggcaaaaata atcttaatgt atgtccatat aaattgtctc gtttctttca
     5161 tttattcact tccttaattc attaataata ctcttatctt attataactt acttttcacc
     5221 ttaagttaac tttaattaat aaaaaaatta ggctagtcta aattattatg tttcattctt
     5281 ctagaatata cttctacgcc aaaaactgtt aaaaaacttt tactttagaa aattggagaa
     5341 attgatcgct taagataaat tcaggacact aaatagtaca ggaagtagac taaaactttt
     5401 aaaagtttta agtctatcta tttggcaagt ttaaaaaata aaaattcggt atcaaaaaac
     5461 tgttaccgat aacatttttg aaagcgttgt cttttgcttc tctatcactt atagtgatga
     5521 cgtcatatta aaacacaagg aggaaatcaa tggatacagc tgcaatttat catcgaccgg
     5581 aaagtgaata tgcatttcta tatacgaata caagatttcg tattcggctt cgtactagga
     5641 aaaatgatat caaaaaagta tatgtgctat gtggagatcc atataccata acaactgaaa
     5701 aatggtatca aaaacaacga ccaatgaaaa aatgtctaag taccaatgtt catgattatt
     5761 gggaaattga agtgacgagc gaaacaagtc gcttacagta tgcgttccat gttgttgggg
     5821 aagataacac ggactgtttt tacggtgatc aaggaatttt tccttatcga gaaaatgtga
     5881 ttactgaacc aaatttttat tttcgaatac cttattttca tcaaatcgat cgattcacta
     5941 ttccagaatg ggtaaaagaa acagtatggt atcaaatttt tcccgaaaga tttgcaaacg
     6001 gggacaaagc aaatgacccc gaacacactt taccttgggg aagtaaagat ccagaccgag
     6061 aagacttttt cgggggagat ttgcaaggtg tgatggatca tttggattac ttgacggacc
     6121 ttggcatcaa tggcatctat ttttgtccca tatttaaagc cacatccaat cataaatacg
     6181 atacgattga ttattatgag gtggatcccg cttttggcga taaaaaactt ttaaaaaact
     6241 tgattgatga agcacataaa agagggatac gtatcatgtt cgatgctgta ttcaatcata
     6301 tgggtgtcca ttcaccacag tggcaagacg ttctcaaaaa cggagaaaaa tcgatctata
     6361 aagactggtt ccacatccgt tttttcccag tagattctta tcagatgact gacttaccag
     6421 aaactgctga aaatatccca tatgatactt ttgcttttac tccattcatg ccgaaactaa
     6481 atacagcaaa tccagaagtg caaaaatatt tgctggatat cgcaacttat tggataaaag
     6541 aattcgatat tgatggatgg cgtcttgatg ttgctaatga agtcgatcat catttttgga
     6601 aaaaattcag agaagcagtt acagaaatca aaccagacat ctatatcctt ggagagattt
     6661 ggcattcctc tcaagcttgg ctgcaagggg atgaattcca tgcagtgatg aattatgcat
     6721 ttacagattc gattaaagat tattttgcaa aaaagaaaat cacagcttca caaatggtca
     6781 gtggaatgaa tcatcagcaa atgctttaca gagatcaggt gaacgaagga acattcaact
     6841 tattagattc tcatgatact gccaggatct tgaccctttg ccaaggtaac aaagaattga
     6901 tgaagtctgt gatggccttt atgtttcttc aaaaagggtc tccttgtatc tattacggga
     6961 cagaaatagg gatgactggt gaagatgacc cggattgccg caaatgtatg atatgggaga
     7021 aggaggaaca agatctagag ctattcggtt ttataaaaga acttgtttct ctaaggaaac
     7081 agttgtctaa gattatttct gaaggttcta ctcagtggct aatagtaaat gatcgtgaag
     7141 ataagctcta tttcacacgg aaactagagg gacagatcat ctatgtttac tttaatcagt
     7201 caaaagaacc ttgggtagta gaacaggaaa atgaggtgat tcttagtcaa aattgtcagt
     7261 tgttagaaga cgggaaagca gagatacgac aatacggatt tttgatcatg tgttgaaaac
     7321 tgttaccggt aacaacaaga aagcgtttgc aaatgaagat ttgtgcccat tataataagg
     7381 gtgtaacagc acgttgaata caaaggagga aatgaagatg aaaaaaagaa gtgtgaaaca
     7441 gttgggttta gggttaatct ctttaggaat cttaggcgga ttagcggctt gtggcaatgg
     7501 taactcttca gcagaagggg aagccgatga aggaaaaacg ttaacagttt ccgttgattc
     7561 tggatataaa gattatataa acgaaataaa aaacactttc gaaaaagaaa acgatgtgaa
     7621 aatcaagcta gtagaaaagg acatgttcga tcagttggaa tcattagcgt tagatgggcc
     7681 tgctggaaag ggacctgacg ttatgatggc agcatatgac cggatcggtg cgttaggaca
     7741 gcaaggacat ctggcggaag taaaactggg aaatgaagca gcttacgatg aaacagacaa
     7801 agcccaagtg acttatgatg gaaaaatcta cggagaacct gcagtaatcg agacccttgt
     7861 tttgtactat aacaaagatt tagtcgatac agcaccatca acgtttaaag atttggagaa
     7921 tctatccaaa gacagtcgtt ttgcttttga gtcagaagca ggaaaaaata caggattttt
     7981 agctaaatgg actgactttt attattccta tggtttaatt gcaggttacg gtggctatgt
     8041 atttggtgat gatggcacaa atccttcaga tatcggtttg aataatgcag gtgctgtaga
     8101 aggaatctct tatgcaacag attggttcaa aaatgtttgg ccaaagggaa tgcaagacat
     8161 aaaaagtgca ggagacttcg ccagccagca attcatgtcc aataaaactg cagcaatcat
     8221 tgacggtcca tggcaagcgc aaacatacaa agaaaacaat atcaattatg gtgtagccaa
     8281 aattcctact ctaaataatg gacagcctta tcaaccgttt ggcggaggaa aaggatgggt
     8341 agtcagcaat tatgctaaaa acaaagacct ctctcaaaaa tggcttgatt atgtaacgaa
     8401 tcaggaaaat caagaaaaat tctttgaaat ggtaaatgaa atccctgcaa accaacaagc
     8461 tagggaaaca gcaaaggcta aaaacgatga attaacaaca gctgtgattg aacaatatga
     8521 aacagctcaa gcgatgccaa acatcccaga aatgggcgaa gtttggacag gtgcagaaaa
     8581 cttgatgttc gatgcagcat caggttcaaa aacaccaaaa gaatccgcag atgaagcagt
     8641 aaaaacaatc agtgaagcca tagaacaaaa atactgatta aaatgataac cgtccatttt
     8701 tataaaaaat ggaacaggtg cttcagataa aagtggcaag agcgagatgg aaaattatct
     8761 tgctcttgtt gccattatct taaaagcagt ttgcggttta gcaagtttag agggggcatt
     8821 cagatgcagc aagataaaac aagcaatatc aacgaaaaaa aaatcacgct ttgttcactt
     8881 attccaggga tggggcagtt tgtaaatggc caagctttta aaggtatcgt ttttttcgcc
     8941 atattcgtcc tattcattgg tcagatgctt ctgggcggat ttcatgcatt ggtaggattg
     9001 gttactttgg gaagtatacc aatggaagat cattcattat ttattttgat tgaaggcact
     9061 ttgcaattca tcattacaat cattttcttg attttttacg gtttgaatct tttagacgct
     9121 tatcgagtag cgaagatgcg tcaagagggc aaaaaaatca atcggacgat gaaggaagtc
     9181 atacataata taggtgatga aggttttcct tatctattga cacttcccgc ttatcttctc
     9241 atgattttta cgatcatttt cccagtccta gttacattgt tcactgcatt tactaattat
     9301 gacttcaatc atattccacc agccagttta attgattggg taggggctaa aaactttttt
     9361 agtatcttct ttttaagctc atatcgtaat actttttttg cagtcttttc gtggaccgtg
     9421 atttggactt tatgtgcaac aactttgcaa atcacccttg gtattctcac agcggttgtt
     9481 gctcaccaga aatttattaa attcaaacgt attttcggta tcattttctt attaccatgg
     9541 gcggtcccag cttttattac tatcatgagt ttttcgaata tattcaacga tagcgtaggt
     9601 gccatcaata ctcaagtgat tcctttcata aaccagcttc cttttgtaga tatcagtgca
     9661 atctcttgga aaacggaccc gaactggacg aaagtagcaa ttatcttagt gcagggctgg
     9721 ttaggatttc cctatatcta tgtgatggtt acaggaatct tacaatcgat ttcagaagac
     9781 ttatatgaag cagcaaaaat cgacggagca aatgcatggc agcgcttttc aaacatcacg
     9841 cttcctgctg tcttcctagt ggcagcgcca acatttatta cacagtatac ggggaatttc
     9901 aataatttct ccatgatcta tctgtttaac aatggcggtc ccggaagcgt aggcggagga
     9961 gctggttcta ctgatattct tatttcctgg atctataaac ttacgactgg aacttctccg
    10021 cagtattcta tggcttcagc aatcacgttg attatctccg tcatcgttat ctgtgtgtca
    10081 ttgctggtat ttaaaaagac aaatgcgttc aatatggggg attaacaatg aaaaaatttc
    10141 attcgagtgc tcgattccaa aaacgacttt ctcacttttt tacttacttt tttcttttaa
    10201 tactgtcagt cattatcatc tatccgcttt tgatcacagc aagtactgcc ttcaagtcag
    10261 gaaatattgc agcttttagt ttagatcttt caagtgactg gactttagga aactttactc
    10321 ggctattcag tgaaacactt tacgggactt ggtataaaaa tacattgatc atttctgtcc
    10381 taacaatgat cgtacaagtg actgtagtaa ctttggcggg ctatacttat agtcgctatc
    10441 gttttatcgg ccgcaaatca agtctgatgt tctttttagt gattcagatg gtaccgacta
    10501 tggcagcatt gactgccttt tatgtcatgg ctttcttatt agaagcgctc gataagtact
    10561 ggttcttgac aatgatctat ataggcggtg gaattccaat gaatacttgg ctgatgaaag
    10621 gatactttga cactgtacca atagatttag atgaatctgc taaacttgat ggggcagggc
    10681 atttcaggat ttttttccag attgtattac cgcttgtcaa accgatgctc gccgttcaag
    10741 cattatgggc ttttatggga ccttttgggg atttcatgtt agctaagttc ctgcttcgaa
    10801 cacctgaaaa tcagactgtt gctgtcggct tacaaacatt tatttctgat gtgacaaacc
    10861 aaagagtctc tctttttgct gctggggcaa ttttgattgc actaccgatt tgtctattat
    10921 tcttcttttt acaaaagaac tttgttaccg gcctgacatc tggcggaaca aaaggatagg
    10981 agcagagtat catgcaaaca tttccgttaa actatttttc cgcattacgt acgccaactc
    11041 aagtgtttgc gggacgcaaa ctattatcat ggcctaaatt tttcttgata tttgtatttt
    11101 tagtttcctt gatggtcatg cctgtgactc ttttttatgc caatcagatc caagcgattc
    11161 cattagaaca atttctatcc gttcattcgc tgatcgatga acaaggaacc caaaaatttt
    11221 ctgaacttga attatctgaa actgggttac aagcatcgca acaaacgata gctgtgaccc
    11281 ctgaaatttt ggtaggtgtg tctttatctg agaaacaaca atccgatcat ggaactttta
    11341 ttgattttga gaaggaacaa tgggtgatcc aacaaaagga taaatctggt atacgccgct
    11401 atacgatgaa ttatagccca tctttccaac cagattctgt acgtacgccc gaagatttcc
    11461 aacgattttt agaaagagaa ttttatgcca gtaatcgtcc aacaatcatt ttatcttata
    11521 gtttaagtct tggtcttgtc ctatttgtca tgacgagtct cattttattc ggggcttcct
    11581 ttttcttgtg gatgacaaga aaaagccaac tttcatccat tcatacattc aaagagagtg
    11641 caaatttgat gctgaatgtt atgggaatag gttccatggc agcagcagtt gtagggttta
    11701 tccactttga ctttatcttg atgctaagtg tccaaacaac gataactgtc ttgctgcttt
    11761 tatgggtttt tgtcaaaaca agattcaaag ataagcgtgt cgaatgacaa gtttaagcga
    11821 tttatgcgac aatgaatgtg aaaaaaatga aaaataaagt agtttttgaa aaatttctga
    11881 aataattaaa cttcttagtt gattcttttc ttttttagta ttaacgtaat agggtagttt
    11941 ttttaaggag gaaaatcatg ttagagaaaa ctttatacaa tcaattattt tcacgttctt
    12001 tctcgctccc tgtcgaagta aattattggg acggtacaac gaaacaatac ggagatacgg
    12061 acactcctcc ccaaatcaaa atcaatatcc atgaagaaat cccaatgaaa gagattacaa
    12121 gtaacgcatc tttggcacta ggagaagcct atatggatca tcgaatcgag atcgagggaa
    12181 gtatccaagc attgatcaac gatgcttatc agcaagcaaa cagcttttta cgaggaaaag
    12241 attatctaaa atggctgcca aagaaagaaa aacatacgaa acaaaaaaac aaagaagata
    12301 tccatgcgca ttatgactta ggtaacgact tttataaact ttggctagat ccaactttga
    12361 cttattcttg tgcttatttc aatacacctg aagatacgtt ggaacaagca caaatcaata
    12421 aagtccatca tatcttagac aaactcttta tcaaagaagg cgaaacgttg cttgatatcg
    12481 gttgtggctg gggaacattg atgttcactg ctaccaaaga gtataatgtc aaggcaactg
    12541 ggatcacact aagtgaagaa caatacgatt tgattaccaa aagaataaaa gaagaacatt
    12601 tagaagataa atgccgtgta ttattaatgg attaccgcga actaaaaggc gaaacgtttg
    12661 atcatatcac aagtgtaggt atgttcgaac atgtaggttc tgaaaatctt gaaggttatt
    12721 tcaaagtggt aaaagattta ttgaaaccaa aaggaacagc tttgatccat ggaatcagcc
    12781 gacaacaagg cggtgcgaca aatgcttgga tcaataaata tattttccct ggcggttatg
    12841 tccctggtgt tactgaatta gttgataata ttacaaaaaa cgatttgcaa ctgattgatt
    12901 tggaaagctt acgtcgtgac taccaattga cattagaaca ttggacaaag aattttcatc
    12961 aagtccaaga tcaagtcacg aaagaaatgg gtgattcctt ctatcgaatg tgggatctat
    13021 atttacaagc ttgtgcagct tcatttgaat caagtaatat cgatgtgatt caatatttgc
    13081 ttgttcaacc tggaaacaat gacatcccaa tgcatcgtgc ataattgagg caaaagtact
    13141 gtttaaagac tatttaaaaa gaagctaagt tttatgatgc tccagaagtg aacttttttg
    13201 tctaacttct aggattgtct ctaaagctta gtttcttttt ctttatattt tcataataaa
    13261 agtttggttc tttaattttt aaacatgaca gaaaattttt tcttcgttat acttgtaata
    13321 gaatactatt ttataacaaa ggggatcgaa tcaatgaaaa aaactgttat ttatactagt
    13381 ttatttgcag ctttgttttt agctgcttgt caatcaaata ccaatcaagg cacatctggg
    13441 aatacgtctt ccagtagcga acagcgctct tcacaggtat cggactcctc aacaaaagca
    13501 agcacatctt cgtctagcca gacaaaaagt tctagtgaag caagtactca gtcagatatc
    13561 aaagtgtctg cagctcgtgc gatcgaattg ttccaagata aatacccaga tactgcaatt
    13621 actagtttgg aattagatag tgattgggga tcttacttct acaagataga aggtgtcgat
    13681 gatcaaaaag aatacgaagc aagaattgat gctcaaaatg aaaaaatgga gacagagaat
    13741 ccagaaactc tagatcgaga tgaacaaaat ggtcaaaaga aaaaagaaga tggtctggat
    13801 gttgataaat tgatcacgat tgaggaagca gcaaaaatcg ccgtcgacaa agtaggaagc
    13861 ggtacggcaa cagattggga tttggataaa gatttaggta ctacttattg ggaagtcaaa
    13921 gtaaaagacg gcaatcaaag tacatcagtc aagatcaata gccaaacagg cgaagtactg
    13981 tcaactgaac aagatgatta agaatttctt cggaaaacaa gagttgattt ttcttttagt
    14041 catgatacac tagacaaaat aacttgtttg aaggagcgat ttacttgagt ttagaaacga
    14101 cctttgccaa tcatatcttt gccaatcctt tgatgaatgc atctggtgtc cactgcatga
    14161 ctacccagga attagatgaa ctggctcatt cagaagcagg agcatttatc actaagagct
    14221 gtacgatcaa tgaacgaaaa ggaaatcccg aaccacgcta ttttgatgtt cctttaggga
    14281 gtatcaattc tatgggactt ccaaacttag gtttttctta ttatctagag tatgctcttg
    14341 cgtatgaaaa agtgcaggaa aaccaaaatc aaccgttatt tttttccatt gccgggatga
    14401 gtgtccagga aaacttggaa atgcttgaga aaatagaaaa aagtgggttt aatgggatca
    14461 ctgaattaaa tttatcttgt cccaatgtgc ctggaaaacc acaactagct tacgattttg
    14521 aggccactta tgaaacactg aaagaagtat tcagcatctt ttctaagccg ctaggaatca
    14581 aattgcctcc ttattttgat tttgctcatt ttgatcagat ggctgatatc ttgaatcagt
    14641 ttccattgac ctatgtcaat gctatcaaca gtgtgggcaa tggtttgtat atcgatacag
    14701 aacaagaagc agtagtcatc aaaccaaaag aagggttcgg cggcattggc ggagagtata
    14761 tcaagccgac tgctttggcg aatgttcgcg ctttttatac acgtttgaaa ccagaaatcc
    14821 aaatcattgg tacaggtggg attcggacag gacaagatgc atttgaacat ctgctatgtg
    14881 gcgcaagcat gttacagatt ggtaccgagc ttcataaaga agggccggaa attttttcta
    14941 gaatcataaa agaattaacg cagattatgt cagaaaaagg atatacgtca attgacgaat
    15001 ttaaaggcaa attacgcacc atttcatagc aaagagacga aggtcatgat tgatcttcgt
    15061 ctttttttat aaaaaacact cgtctgttca ttcctgtgca agcgtactca ttgtatgtta
    15121 gacgaacttg tgggaaaata acagaggcaa aaaggaggat aatatgaatg atgcaacaga
    15181 attagcaaaa gagcttaaag aaaaagaatt tagttttcaa gaactagtta cggatataca
    15241 gaaaaaaata aagaagcaaa atcatgaatt gaatgcgttt gtcgcgcttg aaccattcga
    15301 gagtaattcc cacgagctat tgattcccca gtcagaaaaa gggccattgg ctggtatccc
    15361 atttccttta aaaatgctcg gtcaagaaaa aaagggatgg ctagctactt caggatctcg
    15421 tttatttgaa acacaccgag cttctcatac atctaattat gtgcggcaag cagaagcgat
    15481 aggtctagtt ccttttggac aaaccaatgc gccagagttt ggtttcaaaa atattacaga
    15541 cccagtcatc tatggcccag caagaaaccc ttggaacctt gaatgtacac cgggcggatc
    15601 aagtggagga gctgctgctg cagtttcatc aggaatcgtt cctttagctg gtgctagtga
    15661 tggcggtggt tctattagaa tacccgcttc gttttgcgga ttgattgggc tgaaaccttc
    15721 gcgagggacg atgccagttg gtccatatgc ttggagagga tggcaaggag ctgctattga
    15781 tttcggcttg acagtttcta tgagagacac ggaggcttta ttttatggga tgagaagtat
    15841 ccattccggt gcgccatatc aagtatcgcc agttgagtgg caaacacatc caagaaaaaa
    15901 acggttgaag atagcttttt gtgttgattc accgatccac tcacagatta gtgaagaggc
    15961 agtacttgct gtaaaaaaag ctgtagattt ccttggaaaa gaagggcatg atttgacaga
    16021 gattccttac ccactagatg gtcgtaaact gatcacgtct tattatcaga tgaatggcgc
    16081 agaaacagca gcaatgatcc gctcgattga aacaggcatc cagcgagctg tcagaaaaga
    16141 agaacttgaa cccatttctt ggactttgtt ccaatatggt cagaagctct ctgccgctac
    16201 ttacatcgat tctctgcatg tatgggatga agcagcaatt gtcatggaaa acctacatca
    16261 agaatacgat ctattccttt caccaacgac tgcatttacc gcccctaaaa tcagcgaaga
    16321 tttgcaaagt gatgcgataa gagcacagat ggaacacatc agtgattgtt ctgaagtaga
    16381 aggtttagac gttgtttctt ccatgtttga ggagagcttg aagatcacac cttatacaca
    16441 gttggctaat ctgactggac aaccagcaat cagcttgcct actcatttga caaaagaaaa
    16501 catgcctcta ggaatccaat ttatggctgc cagaggaaga gaagatttgc tatttcaaat
    16561 cggctatcta tttgaagcaa atcatcagtt ccatttacca aatccatgga aatagcagaa
    16621 acaaggagaa gaagaatgaa gaaaaaaaga ttgtttgctc taataactag tgcctttttt
    16681 attttaagcg cttgcggtac agctgccgct aatcaggaag ataacagctg gagtaatgta
    16741 gaaaaaaaga aagaattagt agtagcgact tctggcacac tgtttcccgc atcttattat
    16801 aatgatcaaa atgaattagt tggttatgat gtagatttgg caaaagaggt ggctaaacgg
    16861 ttaaatgtca aaatctcctt taaagaatac aacgtggatg gacaaatcac ttctgtttca
    16921 aaaagagaat cagattttgc tgcgaacgat tttggtatca caaaagaaag agaaaaaaaa
    16981 ttctcccttt cagagacttt caaatattca tttgacagta tgatcgtacg aaaaaaagat
    17041 gattcaggaa tccactcact tgaagatctt aaagggaaaa aagcagctgg cgaacccaat
    17101 actagctata tgcgtttagc ggaaaaacac ggagctaaat tagtcacata tgacaatgcc
    17161 acgaatgatc agtatttaag cgatgtagca aatggtcgaa cagatgttat tctaaatgat
    17221 tattatctgc agaaaatggc cgtatctgct ttaccagata tccctgtaaa aatactggag
    17281 aacgtttatt tcaatgaaaa cgaaagtggt tttttattga aagaaggaaa tgatgctctg
    17341 aaaaagaaaa tcgatggcgt attaaaagag atgaaagaag atgggacgac aaagaaaata
    17401 tctgaaaaat attttcaaac tgatgtttca gtcaaaccga aagaaacgat ggctgagtcg
    17461 gtgagtgagt gatgtacttt cctctaattg atttttccct catgtggact tcacttcctt
    17521 tcgtactgca aggcttggta tacacattag ggattggctt cgtgtcgttt cttttaggaa
    17581 atctggtagg gttgcttttg acagttttag gtcttttgga ttggctgcct ctgaatgtat
    17641 ttattcgttt ttatttatcg ttctttagag gcattcccgc actagtcctg ctgtttctgt
    17701 tatattttgg gttaccttat caattaagtg ccttaacggc ttcagtcatc tgctttacga
    17761 ttaccagcag tgccatttat tggtgaaatc tatcgaggat cactagcagg cgtttcttca
    17821 ggtcagtggg acgctggttt agcttctggt ttttctttct ttggggtatt gaaatatatc
    17881 gttttgccgc aagcttttcg aatctcagta ccggcactca gtaacgtagc tatggatcta
    17941 gtgaaaggta cttcgttagc agcaacaatc acagtgccag aaatatttca aaaagcaaag
    18001 atcataggcg gtcggacatt cgattatttt tctctctata ttttagttgc actgatttat
    18061 tgggtcattt gtatcgggat cgaaaaagtg caacagtatt tagaaaaacg ttctatgcat
    18121 cagtcaatct gaaaaataga aatggtgcta gcgtggctgg caccatttct attttttgtt
    18181 tcttgtatat tctgcctagt attttaaaac catttcatgt aaaatgtaaa tcgaaaatat
    18241 gttcggaggt agaaaacatg aagaccccgc taaccatcga aatagaatcc tcgttatacc
    18301 aatattgcat cgagcaaggc ggcttggttg tagaagaagt cacgatgcct gatgaccaag
    18361 gaattgtaga tacgcttagc tgcttgacaa aatttgatgg aagccgagaa tggcgatgct
    18421 atgagctgaa agtgacaaaa gcagattttc gctctacagc caaactgtca tttgttggac
    18481 attataatta ttttgtttta ccaagtaaac tatatgaaga agtaaaaact gaaatcccta
    18541 caggaatagg tgttttagtg tatcgtccgt acacggttga agaggagatg cctgcctctg
    18601 gtacatttct gattgcgaaa aaagcagcaa agaaagagct tgctgtaccg gaagaagctt
    18661 tgacaaaccg cttcatggct tctttattca gggaagtaag aaaagcaaag caaatggagc
    18721 gaggcgtaaa atattttcca acagatcaat tgtacaaaga attgaaaaga cgttcagaaa
    18781 atcaagatcc attttctaac gaacgatatt atgaacggtt tttagaggaa atagctagtc
    18841 aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca gcatgattat gaatatttga
    18901 ggcaacaaca gcagatccgt cggttgccta cagagccatt ggagtgaaaa atatgtacta
    18961 tccttattta agaggaaaac aatttgacct attagcatta aaagaagcac tgtcgcgagg
    19021 attgctctcc aataaaatcc aaccagtgat cgaaccagta cgtgattccg ctacgcttaa
    19081 aaatgtgatc gaattatttc aaaaaaagca ccatcgaatc gctgtgatcc aaaatcccca
    19141 agtaggacaa ttcaaattat tcgatcagca tgtgcacagt tggacagtca aagaaaatag
    19201 ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc agtgaagtac tggattcacc
    19261 tccagcgttt cttgttttcg atggacagca ctacccaaaa gatacagagg tgtggaaaca
    19321 gttggcagga gttgattcga aatttttgat accagatact agtcgctttc gcatctggct
    19381 gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg agaaagcatg ttgaaagtta
    19441 tgcagataaa acagatgact tttttagtga tgactatctt tttttccacg cagacggata
    19501 tattggattt tctgatttca cgatcgaagg tagccgttat ttcgataaag ggttccctag
    19561 ccgagcgctt gctatccatc taacgtatat tgatgcttac ggcaatatac gcgtgaaaca
    19621 ttttgtctct gacagtaacg actctgctaa agaccaagca ttgaagtttc ttgaagcagg
    19681 agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa ctcttgatta cttcaggaat
    19741 gattgaatta ttggctcttt accagcaaca aaaattccct ggactgggag tgttgaaaaa
    19801 gtggtcactt atgcatcatt tggaacttat tagtcacctg ctagatcatc caaaagattg
    19861 gcttaaatat tattcaaaag ttcaggaact atatgaagtg atccaatgat tagataatgt
    19921 aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat tcggcaaaca aggaaagaag
    19981 aagtagaaga agtggtagaa atcattaatg ccgcagcgga tttcattgct tcgcaaggct
    20041 ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac gattttgaac gatattgaaa
    20101 gaaaagaaag ctatgtatta atagtggaag atcaaattgc tggaacagct gcattagtct
    20161 caagcgtgga tcccgtttac acaaaaatag atggtagttg gaaagaatca actggtcctt
    20221 atttatcgat tcatcgtgtc gctttagcag aaagtactcg aggaaaaaaa ctagcaaaac
    20281 cgttcttaga acatttgttg acgattgcag ccagcaaaaa tatccaagat atccgtatcg
    20341 atacttatcc agctaatgaa ccgatggaaa agaccatcta ttctgctgga ttcacttatc
    20401 aagggatgat tgaatttcct tttgaacacg gcgaaagaaa agcatatcaa ataatcatca
    20461 attgactata aaatcatctg agcaaagata aagacattaa cactactgaa aaaaacaatc
    20521 atatgatagc caagaggttg taacagaagt ggccagcttc aaaaaacaag aaagaatttc
    20581 cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt ttacgaaata gctgctttct
    20641 gaaactccct tcatcgagag gtcgtgacat agcttttgtc acgacctctc ggcttatcct
    20701 acgagtatta ttcgatacat ttatatcaat aatccttttt ttattttatc tgcagcttct
    20761 tggaacgctc catcgtttga cacgactaaa agtgagggga tagctttcaa acgtttcaac
    20821 tgtgctaaat cttctacata aaccgccagt ctggctgaaa tctgttcaga tgaatctcca
    20881 cgttttttca ggcgcgattg caatgtttct ttagagatcg taataaagat aggacggact
    20941 accgtcccga aatactcata taaatgccaa aaaccatttg ctgtcaaaac ggcgtaacaa
    21001 tttcctactg tcagtttctt tttaatttct gctttactgg aaccatattt atgttgagca
    21061 tattggtccc actctgcaaa ctcatttccc ttgatcatct gattgaatgt attgtcagaa
    21121 acaaaataat aatcctcttg atctttttcc ccaggtcttg gtgatcgatt cgtgtaagta
    21181 atgatctttt gttccggctg aaaaatagct tctgcgagtg aagtttttcc agaaccagaa
    21241 ggaccgataa atacatagca ataattcatt gtcatttctc ctttgatgat tgaacaagtg
    21301 aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa ctttgctttt ttgtatatta
    21361 taccagcaag ccagtcacaa tagcacttaa aaagctgact aaagtcgctg ttagcaaaat
    21421 tttcaatccg tgttttgcga caagatttcc tttttcttca tttaatccct tcatcgcacc
    21481 agaaatgatc ccgatagaag agaagttggc aaatgaaacg aggaagacag atataattgc
    21541 tgttgtacgc tcagtaaaat ggaaattacc atttgctaat tctgtcatag caacaaattc
    21601 atttgtaacc agtttagtcg ccatgatact accggcatcc actgcttctt tccatgggat
    21661 tccgctgatg aaggcaagag gggcaaagat gtaacctagg atctcttgga aagtgatgcc
    21721 gaatatcccg tggaagatag cattgatcat ggcaatcaaa gcaacaaaac cgataagcat
    21781 agcagcaacc gtgatcgcca catggaaacc atcaagaatg tattctccca acatttggaa
    21841 aaatgtctgt tctttgtttt cttcaatgat cagctcatct tctttttctt ctaatacata
    21901 gggatttaca attgaagcca gaatataacc gccaaataag ttcaaaacaa gggcagtgat
    21961 cacatattta gaatcgatca gtgccatata agatccgacg atcgacatag aaactgtaga
    22021 catagccgat acactcatgg tgaacaaacg tttctcagat aggaagggta attccttttt
    22081 tatggaaata aaaacttccg attgtcctaa aatagcagaa gctactccat tataagattc
    22141 caatttgccc atcccgttga ttttacttaa tcctagacca acagctttca taaataatgg
    22201 aagaatcttg atatagcgta gaataccgat aatagcagaa ataaatacaa ttggcatcaa
    22261 tacggaaaga aagaaaggtg cacttccttt atttgctaca ccggcaaaaa caaagtcaac
    22321 accttgccca gcaaaagcaa gaagatgatc aaacattttt gccaatacag aaacaaccgc
    22381 tgtaccaaaa gttgtacgta ataatacgaa accaagaaca agctgtaaaa cgaacatcac
    22441 aattagtggt ttcaatttga ttgctttttt gttactgctg atcaaaaaag caagccctaa
    22501 tgtgacaaat aaaccaataa tacctaataa ataactcaaa taatcactcc tattccaaat
    22561 tccgttttat tttacctgtt ttgcaaataa aatactaatc tttagtgaaa ttttcattac
    22621 ttttttagag tgaaacgcat tcattttcca ttagaaaata aagcgaacaa aataaaaggt
    22681 ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg aaactatcga ttaagctaat
    22741 agtttcgttt tcaacattta taagtaaact ttttctattg gtaatataga ttttcggaag
    22801 ggtagacagg tgcgcaggta atatcgattc ctagtttttt caatgtttgt tcatcatttt
    22861 tattgaccat cacagtagag tgggcttgtg ctccctcaag ttcagctagt ttatcataag
    22921 caacttgagc gattggattc gttactgcac tgattgataa agcgatcaag acttcattga
    22981 tgcttagtgc atccaaagag gaatgaagtg tctttgattt caatccttgt attgtttcaa
    23041 gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg ggctaaatgt tttactgcat
    23101 ttaagataac tgccgctgac gcatccatca atgaacttgt acgcccggta atgatgactt
    23161 catctggtaa ttcgattgca atcacagcag aaggatcttt tgtaggactt tgcgcttgaa
    23221 tcttatctgc atagtcatgg gcagggacta ccacgtttcg gtcttcagga cgtaaaccga
    23281 cttcttccat aatcagtttg attcgattaa ctgcttcttc gtcgatcaat ccttttttga
    23341 agtcgcattc tgtttggaaa taacgacgga tgatttcttg tttagaggct tcttggacaa
    23401 cggtatcatc cacgatccca aatcctacac gattcacacc catgtcagta ggagattgat
    23461 aaacagattc tttaccagta attttttcaa taatacgttt gattacagga aatgtctcaa
    23521 catcccgatt atagttcaca gctactttat tgtaggcatc aaagtggaag gaatcgatca
    23581 tattgacatc ttttagatcg actgtggctg cttcataagc aatgttcagc ggatgtttca
    23641 acggaacatt ccatactggg aaagtttcga acttggagta accagctgca tgtccttttt
    23701 ggctttcatg atacagttgg ttcaaggaag tagcgagctt tccacttcct gggccaggag
    23761 ctgtcactac aacgattggt ttagttgttt caatatactc attttttcca aaaccatctt
    23821 ctgagacgat tttttctacg ttgactggat agtcttcgat ttctgagtgt ttatagacct
    23881 tgatgtttcg tctttctagt ttattgataa agactttcgt ggcaggctgt ccactatagc
    23941 gagtgataac gacactgttg attaataagc cataaccacg taattcatcg attaaccgca
    24001 agatatccat atcataagta atcccataat caccacggat cttattacgt tcaatatctc
    24061 cagcatagac gcaaatcagt atttcagctt gatctttcaa tttttgcagc aatttgattt
    24121 tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg cttgtcgtca actagcttcc
    24181 caccaaattc taaatataat ttgtcatagt ggttcactcg ttccaaaata tacgctgact
    24241 gctcttcaat atatttttct gaatcaaaac caattttttt cactaggttc cctccttaag
    24301 tactaaagca ttttttcatt tttttgagga ttcgtcaact ggaagagcga aattcctcaa
    24361 aaaaacaaag aaaatggttt aaaccaactt gtctagatag ttattcattg gtatgatagt
    24421 ttttgataag gaaggtgcta catgaaaaaa agacagaaaa agaaaaacgc atataaacac
    24481 tatatccgtt cgatttttac cggttatgaa agaatgcttg aagatcccga attggaacaa
    24541 ttgacgttta cttatctaaa cgaagaaacg cagctaactc gtgatgagca tcagcgcatt
    24601 catttcacaa caagagatct gcctagtaaa taaaaaataa gataaaggtc atcggattcg
    24661 attctgtcct atcagatgga aagactagtt gatcgaaaaa aaggctgtac caaattttgg
    24721 tgcagcctgt tttatggagc attgcccgct cttttttata aca
//
LOCUS       DAZAFF010000036        24687 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000036 DAZAFF010000000
VERSION     DAZAFF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.036"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(541..1566)
                     /locus_tag="ACG9BB_001393"
     CDS             complement(541..1566)
                     /locus_tag="ACG9BB_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2837338.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1841..3526
                     /locus_tag="ACG9BB_001394"
     CDS             1841..3526
                     /locus_tag="ACG9BB_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFN2837339.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3540..4823
                     /locus_tag="ACG9BB_001395"
     CDS             3540..4823
                     /locus_tag="ACG9BB_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2837340.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4893..5762
                     /locus_tag="ACG9BB_001396"
     CDS             4893..5762
                     /locus_tag="ACG9BB_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2837341.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5759..6595
                     /locus_tag="ACG9BB_001397"
     CDS             5759..6595
                     /locus_tag="ACG9BB_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2837342.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6621..7688
                     /locus_tag="ACG9BB_001398"
     CDS             6621..7688
                     /locus_tag="ACG9BB_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFN2837343.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7685..8512
                     /locus_tag="ACG9BB_001399"
     CDS             7685..8512
                     /locus_tag="ACG9BB_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFN2837344.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8557..9582
                     /locus_tag="ACG9BB_001400"
     CDS             8557..9582
                     /locus_tag="ACG9BB_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN2837345.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9579..11762
                     /locus_tag="ACG9BB_001401"
     CDS             9579..11762
                     /locus_tag="ACG9BB_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFN2837346.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11762..12892
                     /locus_tag="ACG9BB_001402"
     CDS             11762..12892
                     /locus_tag="ACG9BB_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN2837347.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12889..13566
                     /gene="pgmB"
                     /locus_tag="ACG9BB_001403"
     CDS             12889..13566
                     /gene="pgmB"
                     /locus_tag="ACG9BB_001403"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFN2837348.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13650..14177
                     /gene="msrA"
                     /locus_tag="ACG9BB_001404"
     CDS             13650..14177
                     /gene="msrA"
                     /locus_tag="ACG9BB_001404"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN2837349.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14378..15064)
                     /locus_tag="ACG9BB_001405"
     CDS             complement(14378..15064)
                     /locus_tag="ACG9BB_001405"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFN2837350.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15144..16682
                     /locus_tag="ACG9BB_001406"
     CDS             15144..16682
                     /locus_tag="ACG9BB_001406"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFN2837351.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16802..17269
                     /locus_tag="ACG9BB_001407"
     CDS             16802..17269
                     /locus_tag="ACG9BB_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFN2837352.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17803..18231
                     /gene="rplM"
                     /locus_tag="ACG9BB_001408"
     CDS             17803..18231
                     /gene="rplM"
                     /locus_tag="ACG9BB_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFN2837353.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18247..18639
                     /gene="rpsI"
                     /locus_tag="ACG9BB_001409"
     CDS             18247..18639
                     /gene="rpsI"
                     /locus_tag="ACG9BB_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFN2837354.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18727..19953)
                     /locus_tag="ACG9BB_001410"
     CDS             complement(18727..19953)
                     /locus_tag="ACG9BB_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2837355.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(20025..20327)
                     /locus_tag="ACG9BB_001411"
     CDS             complement(20025..20327)
                     /locus_tag="ACG9BB_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2837356.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            20875..21594
                     /locus_tag="ACG9BB_001412"
     CDS             20875..21594
                     /locus_tag="ACG9BB_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN2837357.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            21596..22438
                     /locus_tag="ACG9BB_001413"
     CDS             21596..22438
                     /locus_tag="ACG9BB_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFN2837358.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            22435..23385
                     /locus_tag="ACG9BB_001414"
     CDS             22435..23385
                     /locus_tag="ACG9BB_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFN2837359.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            23400..23633
                     /locus_tag="ACG9BB_001415"
     CDS             23400..23633
                     /locus_tag="ACG9BB_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFN2837360.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            23964..24548
                     /locus_tag="ACG9BB_001416"
     CDS             23964..24548
                     /locus_tag="ACG9BB_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2837361.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT         8129 a         4205 c         5043 g         7310 t
ORIGIN      
        1 gtctaaaata atttgtctaa ctagaagaaa agtgggaaag gatcttaaca acatcggttc
       61 gtatccaatt aaggtatcgg aacgatcatc ttccaagaga agtcaatcaa caaaagtaaa
      121 gatagatcat tttttgaatg caaaataaat tgatgggaag atagagcggt tatttttaat
      181 attttgctag ctattattga ataattcctc gaataaaaca aaaccagtag acatgtctgt
      241 tattctattt atgttttaga gataagtatc cccaaataac atggattgaa ataagggtaa
      301 atgtgtttaa aaaaacgaac tataaaaact tatacacaac gtggttaaaa atagtttttt
      361 cttccctgtg gaaaaaatag tttaacacaa acattatagt tatccacagc tatctaaaac
      421 agatggaaaa taagaaaaat tcgctaaaat accgggaggg ggtaacgagg gatgagtgat
      481 tgaaaaaaga acagagtaac tgacttatcc tcagtgtttc tgttctttta taattctgtt
      541 ctacatttct tgttgttgga tcgaatctgt gagagtaatc tcggttgaaa gaatgattct
      601 tttttcttca gggtattggt ggtggattag ttggaataaa agttctgtgg ctttgattcc
      661 taactgattt gtcggaatgt ctatccgagt caactgtggc gaaaataggg aggacaataa
      721 gggatcactg aatcctgcga tcgaaagatc atcaggtatc tgctttccag attgttctgc
      781 cactttgtaa atactcaatg attttaatgc atcggtagaa aaaatcgcag ttggtctttc
      841 tcgtagcttc attaaatcat tcattgctgc catgctatct tctaagcgaa agccgccgtc
      901 tataatccat tcattacgga tatctaggtg gtgatcaaat aagcattgac ggtaaccatt
      961 gagacgatca ttggatacat aaacttctgt cggagcatgg agacagccga tatgacggtg
     1021 tccattcttg atcagtgttt ccaccaaatc gtaactatcc tggaaattat ccgtgtccac
     1081 tgagtagatg ttcgtgtagt cgtttccgac ttttccgatt accacaatag gaatattgta
     1141 ttgatccaat tcaaaaagaa gtgtttcatc catcgtagag cttaaaataa taattcctcg
     1201 gatcattttt tcttggatct ttttcgcgca tttttctagc tcttcttctg gtgtttttgc
     1261 cgtttgtaga atcaagtcga agttattttg ttcagaaacg gcagaaatgg attgaataac
     1321 atctgaaaaa aaaggattgc cataggtcgt atgagaagta cgatgagata atgctagaat
     1381 cgcgtcaaaa ccatttcctg acagtcctct tgcaattttg ctcggttcat aattcaattc
     1441 tttgattgct tgaagtacac ttgcttttgc tttatcagag atatttggct ggttatttaa
     1501 tactctggag acagttgatt ttgagacatt agcttttttc gcaatgtcat aaatagtagg
     1561 tttcaatttt atatgcacct cctttttggg agcgttttag aataattcca tagtacaata
     1621 gacattcttt tttgtctatg attttgaaaa tttaggaata gcttgcatga tcatgagaga
     1681 ccatattttt ttaagaagct attaattatt gatacataag ctttcttatt tgtatttttg
     1741 tgtatatcaa aattatttaa aaaaatgcta ttgacagtaa gcgttttcca taatatgatc
     1801 aaatcgttgg gaacgctccc aaaaaagaaa ggggaatccg atgtatacaa aacttttaca
     1861 agcgatttat cgcgactcag atttgtctga gaaaaccgcc tctatgattg aacaaaaagt
     1921 cagtgtttat cagcagaaga aaggccaggc aggcaaactg cattgggacg aagaagatat
     1981 tttcttgatt acttatgggg atcaattttt tgaagagcag caaccaactt tgcgtacatt
     2041 taagaaattt tatcaaaagt atttggcaga tacatttaga gttgtacatt tcttgccatt
     2101 tttcccatat acatctgatg atggtttctc cgttgtaaat tatcaggcaa tcaacgaccg
     2161 tttgggcaca tgggaagata tagaagaaat gagaagcgaa acacggttaa tgttcgactt
     2221 tgtctgcaat catatgtctg ccgaaagtac ttggtttcag gaatatttaa acttgaatga
     2281 tacgtacaaa gattttttta ttgatttgcc actggataca gatacaagca gcgtgactag
     2341 accaaggacc agcccgttac ttacaacgtt cattgacaaa aacgggaaag aaaaacacgt
     2401 atggacgact tttagcgaag atcaagtaga cttgaatttt aaaaatccag aagttttatt
     2461 gaaaatgatc gatattcttt tgttttactt agaaaaaggc gcttcattta ttcgcttgga
     2521 cgctgtggga tttttatgga aagaactagg aacttcttgt atacacctgc cagaaacgca
     2581 taaaatcatc caattattcc gtgcaatcgc tgatgaagca gctccaggaa cggtattgat
     2641 cactgagaca aatgtgccac acaaagataa tatcagttat tttgggaatg gcgagaatga
     2701 agcacagatg gtctatcagt tttcgctacc acctttgacc cttcaagcga ttcgttcggg
     2761 taatacaagt aagttgaaag aatggttaaa aaccatcgat ttcgcatcga aaaaaacaac
     2821 tttttttaat tttttagcct cccatgacgg tatcggattg aatccggtac gaggtattct
     2881 gccggaaaaa acgatccttg aattagtgga acagctgaag aaagagggta cccgagtaaa
     2941 ctacaagaaa aacccagatg gaacgaccag tccatatgag ataaacgcta cgtatctaga
     3001 tgcgttaagt tctccaacag atacgacgga atggagaagc aaacgctttt tagctgctca
     3061 ttcatttttg gtaacgttac caggcgtgcc ggcaatttat attcaaagtg tgctgggaag
     3121 tcgaaatgat tacaaaggcg tagaaaaaac aggagagaat cgaaaaatca atcggcaaaa
     3181 atttttgtta gcagatattg agaaagagtt gcagacagat ccattaagaa cagcagtata
     3241 tggtgaattg acccagatgc tatctattcg aaaaaaagaa aaagccttcc atccgaatag
     3301 tttgatttac ttactggact cagcagattc ttttatcagt tttattcgcg aagcgaagga
     3361 agagaaagtc ttagttatcc ataatttttc caatcaatca gcaggttatc ttttaccaaa
     3421 aggagattac ctagacctgt tttctggaca aattatccat tgcagtcaag aacgaaagtt
     3481 gatattccag ccgtatgaat atatgtggct gaaaataata aaataagagg tggaaaaaaa
     3541 tgaaaaaaat tattgttact acctgtctgc tagctactgg atttcttctt actgcttgtg
     3601 gtgaaagtca aagctctaac gaatcacaac agacaacgat tcaattcatg cactcttctg
     3661 tcgaacagga acgattaagt gtgatcaatg aactggttgc tgattttgaa aaagaaaatc
     3721 cagacatcaa aatcaagcaa gtgccggtag aagaatcagc gttcaataca aaaatcgtta
     3781 cgttagctcg ttccggtaaa ttaccagaag taatggaagt cagtcaggat tttgctaaag
     3841 tgatggataa agatgaactg attgatcaga cggcagtcca aaacgttatc gaggaagctg
     3901 gagaagataa ttattatgac ggagccaaaa accttgttcg ttcagaagat ggaaaaagct
     3961 atattgctgc accaattagc ggctgggttc aaggtatttg gtatgacaaa gaaaaattat
     4021 cagaagctgg tttttcagaa ccagaaaatt gggatgatat tttaaaaatt gctcaacatt
     4081 tcacagataa agagaataaa caatatggaa ttgctatgcc tactgctgaa ggaacaatga
     4141 gtgagcaagc gttctctcaa tttgctttgt caaatggagc aaatgttttg gatgatgaag
     4201 gcgaggtaac gatcgatacg gaaaagatga gagaagcatt gtcgttttat caaaacttat
     4261 cccaatacac gatgccaggc tctaatgatg tgacagaaat caaagatgct ttcatgaatg
     4321 gaacagcacc aatggcaatc tattcaactt acattttgcc ttctgtttat gaagcgggga
     4381 actcagaaaa tatcggtttt gctatcccaa cacaaaaaga tcaagctgtc tatggcacag
     4441 tatctggatt gacgatttct tctggtctag acgagaaaca aaaagaggct gctgaagcat
     4501 tcacagcttt cttatctcaa ccgaaaaaca tggaaaagtg ggtcttgatg tctcctggtg
     4561 gtgctcaacc agtgaataaa caagtagtcg aactggatgg ttacaaagaa aatgaagtca
     4621 tcaagtcttt tggcgagctg ccatctgaga ttgctagtgc ttttgatgaa gtacaagtct
     4681 ttggactagt cggggaaaag aatttcacga aaatgggaga tatcacaagc tcaggtgtga
     4741 tcggaaaagc agtgaaccaa gtaactgtcg gaaacgaaga tgtcgatcaa gctttagctg
     4801 acgcacaaaa aaatacgaaa taacaagaag ttctgccaat ggtgttcgaa aaaagtagat
     4861 agctattggc agattatcag ggggaggttt caatgaatcg tccaaggaaa cattctgact
     4921 taaaattagc catgggactt ttagcaccaa gcatgctttt attagctgtt ttagtgctga
     4981 ttccaatgct ttccaatatt cagatcagtt ttttcgatat gccgctgaat cctaactcag
     5041 acgccgtttt tatcggatta agcaattatc ttgaaatttt aagtgatagt gaattttatc
     5101 gttctttagg tttaacagtt gcttacacat tcttagttgt agcaggcagt acagctttag
     5161 gattagccgt agctattttg cttaaccgca agttcagatt taggaaaacg gtgcgttcac
     5221 tgattttatt aagttatgtt acgccatcta tttctttgat ttttgcttgg aaatatatgt
     5281 tcaataatag ttatggaatc gtcaatgatc tgttagtaga taggttgcag cttttcaacg
     5341 aagcaccgct gtggttcgac cagccagttg ctagcttctt tatggtcgtt ttgtttgcta
     5401 tctggagata ctatccgtat gcgtttattt catttttggc tattttgcaa acagtcgaca
     5461 aaacgcttta tgaagcagct gaaatggacg gtgcaaatgc ttggcagaag ttcaagatcg
     5521 tgacactgcc ggcaatcaaa ccagtactag caactgtgat tgttttacga tctatctgga
     5581 tgttctatat gtttacagat gtctatctac tgacgaataa agtcaatatc ttaggcgttt
     5641 atttgtatca gacagccttt gcgttcaatg atcttggaaa agcagcagct atatcgattc
     5701 tattgttcat cattattgca gcggtcgtct tgctctcacg aaagaaggtg gacttgaaat
     5761 gaaaaataaa aagagcagga aaatcttatt ttacgcgacg attttgttgc tattagctgt
     5821 caatttgttt ccatttttga tcatgctgat gacatcattc aaggattcac aagaagcaat
     5881 tgcaacgaca caaactattt ttccgaaaga atggactttt gaacattatc aggatatttt
     5941 caatgctgag atttttccat tcatcatgta tttcaaaaat agtttagtga tctccttgat
     6001 agcctccgta actgcagtag tgattgggat cagcggggca tacgctttgt caaaactacg
     6061 cttcaaagct cgctcgaaaa tcaatgctag cttttacacc gtttatatgt tttccggtat
     6121 tttgatcgtt gttccattat ttaaaatcat ttccggtcta ggtttgtatg ataccaatac
     6181 agcactgatt atcacgatgg tcgttcaaac acttcctact gcgatattca tgctaaaaag
     6241 ttattttgac acgattccga ctgatattga agaagcagca atgatcgatg gtcttagccg
     6301 gatccagatc attattcgga tcattatacc attggctatt tctggaatca tttctgtctt
     6361 cgtttattgc tttatggttt cgtggaatga ttatttattt gcttcgattt tcctgtcgac
     6421 ctccgaaaaa tttactttac caatcggttt gaacacatta ttcagtacac cagactacat
     6481 ttggggacga atgatggccg cttcattggt gaccgcttta ccagtcatca ttatgtatgc
     6541 gttatctgaa cgatttatta aaggtgattt agtcgaaggc ggcgtaaaag gataaaaaga
     6601 aaagaaagag gaaagaaaag atgaaaaaat tagttgcatt agaaccaaga atagccggat
     6661 tcaaagatta cgaagaccgt gagatcgaaa agaacgaagt acgtatccga gtaattttcg
     6721 ctactccaaa accaggaaca gaaattgtcg actttagagg attaagtcca tttatcgatg
     6781 aagaattttc agaagaatgg cgcctattct tgccaagaga agaaggaagc caacgcggtg
     6841 tggtatttgg cgaatttcaa ttaggaaata tgattgtcgg tacgatcgaa gaaacgggca
     6901 gtttggtgac agagtatcaa aaaggagagt tagtctgcac ttatggaccc attcgtgaaa
     6961 cgctgattac aaacggtgtt gataattata aactacgtaa acttgatcgg ttggatcaat
     7021 ggaaaaatgc agtatgttac gatcctgctc aattcgcttt aggtggtatc cgggaagcac
     7081 atgttcgagc aggtgatgct gtagcaatct tcggtttagg cgctatcggg caaattgcga
     7141 ttcaattagc taaaaaagca ggtgctacta ccatcgttgg gatcgatccc atagcgcagc
     7201 gtagagaatt agcagaaaaa tacggtgccg attacacgct agatcctatg aaagcagatg
     7261 tcggttttcg tctaaaggaa ttgacagata aacgtggagt agattcagtt attgaaacga
     7321 gtggcagtgc tgccgcatta caagcttctt taagagggat cggctatggc gggatcattt
     7381 cttatgttgc ctttgctaaa ccttttccag ctgggtttaa tcttggtcgc gaagcacatt
     7441 tcaataatgc aaaaatcgtc tttgctcgag catctagtga accaaaccca gattacccaa
     7501 gatggaaccg taaacgaatc gaggacacat gttgggaatt attgatgaac ggttacttgg
     7561 attgtgaaga aatcattgat ccagttgtgc catttgcaga aagcgcagaa gcttacatgc
     7621 gttatgtaga ccagcaccca gaagaaagta tcaaaatggg cgttcaagtc cgaggaggag
     7681 aacaatgaaa ttcggtacgc aagatcaagc atttttccca agagacattt tagaaaaatt
     7741 tagatttatc aaagactgtg gatttgaaga atacgaaatc gacggtaagt tgttaataga
     7801 aaatatcgac acagtcaaac aggcaatcaa agaaacaggg atcccagtag tgacagcttg
     7861 taacggctac gatgggtgga tcggtgactt tatcgaagaa cggcgtctta atggtgtacg
     7921 tcaaattgct gagattttgc gtgcactaag cgaggttgga ggcaaaggaa tcgttatccc
     7981 agcagcttgg ggaatgttca cttatcgatt accgccgatg gtttcacccc gaagcaaaga
     8041 aggagactat aaagcagtca gcgaatcact tcgttattta gataaagtag ccgaagaaac
     8101 aggtacggtt atttatttgg agcctttgaa ccgataccaa gaccatatga tcaatttgtt
     8161 agcggatgcg cgtcattaca ttgactctaa tcaattgaaa catgtgcaga tcatcgctga
     8221 tttttatcat atgaatattg aagaagatga tatttcaaaa gcattgacta cgcaaaaaga
     8281 gtatgtgggt catatccatt tagcagataa tcatcgctac caaccaggat ctggcgcaat
     8341 tgattttact cgccattttc aaacgctact tcaaaatgag tatgaaggtt atatggtata
     8401 tgaatgtcgt ttgcgaggag aaaatgaagt gaaaatttat caggaatcat tgcaatactt
     8461 gaaaaactgt ctggaaacag cgcgtcattt aaatgattta gagaaagaat agagaaagaa
     8521 taacaataag taaataagca aaggagggag aaataaatga agaaagtagc aatcataggc
     8581 gctggacaag tagcagaaaa agtacatgct gcttattatc aaaaaagatc agatgtctta
     8641 gaattggccg ctgtggttga tccagtcagc cagcgagcag aagacttttg ccgacgtaca
     8701 ggattttctc atgcgtatac atcagttaca caaatgctga atgagatttc tcccgatatc
     8761 gtgagtgtct gtacacccaa tcgctttcac ttcgaacatg tattggctgc cctcgaagct
     8821 aatgcttcag tgatgtgcga gaaaccacca gctatgacag ctgatcaagc aagaaagatg
     8881 gcagagacag ccgataaaaa cgggcagatt ttggcttatg gattccagca tcgtttttct
     8941 gacgaagcaa caatgatcaa gaatcatctt gatgttctcg gggatattta ttacgtagaa
     9001 gcgaatgcgc ttcgtcggtc tggtgttcca ggctggggaa cgtttatcga caaagaactt
     9061 caaggaggcg ggcctttgat cgattatggc attcacatgc ttgatacagc gatgtatatt
     9121 actggatttc ctaaagtaaa aagcgttcgg gcgtacagct tccaaaaaat tggtacgaaa
     9181 aaaaattcag ggaaattcgg gaaatgggat ccaagcaaat tcacagtaga agatgcaatt
     9241 tttggcacac tggaattaga aaacggcaag atcatccaac tgaatacttc gtttgctttg
     9301 aatatcagag aagacaaact gaatgtggct ttttgtggcg atcaggcagg tgctaatctg
     9361 tatccaggcg aactattcga agatgtagat ggaaagctgg tttccttaca aaagaccacg
     9421 ccagttaatg acccacatga actaagtatg gctcattttg tgaatcgggc gctcggtgac
     9481 cagactcaga tgattgcaga tggctggcaa ggatatgtca tccagaatct ggtggaaaat
     9541 ttatatcgtt cggcagaaac aggagaagtt attaccttat gaaagtagaa gaaaaacagt
     9601 ttgatttaga gtatatcaat aagtatgcga gtcagatgac cattggaaat ggattttttg
     9661 gtatcagagg aagccaggag gaagactatc ggaatcaagt aaggggaatg tttgcagctg
     9721 gtgtatataa ccgaccttta ggagcagaat cagccgagtt agtgaatctc ccagatgtca
     9781 tcagatggga aataaaaatc gacggtgaac atttttctct tgagacgatg aaagttcttg
     9841 catacgagcg ggcactagat ttagaaatgg gagagcttaa acgctatgcg ctcattgaaa
     9901 ctgctcaagg aaaaaaagtg gaagtcatta cttgtcgtgt tgctgatcaa aataatctcc
     9961 atcaggcagc cctgaaagtg gtcatcaagc cgttgaccga agactgccaa gtagatgtga
    10021 aaacgggtat cgatggacag caaaccaatt ttggtacaca gcagttggtt gaaaaagaac
    10081 tgcgagtatt tggtgacgat ttgatcagtg catcctacca gacaacagaa agtaaaattg
    10141 atattgggat tgcagctaaa ttcaataaag caggtgtatt ccaagcaaaa aatcgccgga
    10201 tcaccgcttc cttttcagaa aagatcagaa aaaatacgtc ctttacgctg gagaagatca
    10261 tcgtgctctc tacctctctt cagcaaacag acccgtttaa gaaaagtatt gcccaagccc
    10321 agaaaagcgt tagttataca gaaattttag agcagtcgat gtcttattgg acagattttt
    10381 ggcatcagca tcggataacg atccaaggcg attcttcttt cgatcagtta gcgatggact
    10441 tcgctgctta ccatttacag attatgactc cccgcaatga tccaagtgtt agtgtagggg
    10501 cgaagggact aactggcgaa ggttataaag ggcatgtttt ctgggatacg gaaattttta
    10561 tcctgccttt ctttttatat cagtttcctg aagtagcaaa gaatttgctg ctgtaccgtt
    10621 atgatcgatt agaaggcgca cggcaaaaag caagagatta tcactatcaa ggtgcacttt
    10681 tcccatggga aagcgcacac actggtcagg aagagacacc caaatttgct gcattgaata
    10741 tcaaaaccgg aacaagacaa gaagttgctt cagctgccgc agaacatcat atcagtgcag
    10801 accttgctta tgccgttcaa gagtatgtcc aagcaacaga ggatcaatcg tttttagaaa
    10861 caaagggaaa aacgctcatt catgaaacag cagctttttg gctgagcaga gctgtaaaag
    10921 tcgatgatca tttagagata cacagagtca ttggtccaga tgaatacaca gaatatatcg
    10981 acaataatgc atatacgaac tatctggcgc attataatgt ggccttagct gttaaatatc
    11041 aagctggcga tccgcttttt ttgaaagaat gtcaaaattt tttagataag ttatatttgc
    11101 cagtgcctaa caaagaacag ctgattccgc aagatgacac gtatctcagt aagccagtta
    11161 ttgatttgac cacttacaaa gaacgtcctg gcagccagtc tattttactt gattatgccc
    11221 gtagcgaagt aaatgaactc caagtcagca agcaagcaga tcttgtcatg ctgttatact
    11281 tattacctga gtatttttct caaccaatcg tttctaagaa tttgcattac tatgaaaaca
    11341 aaacaatcca cgactcatcg ttgagtaaag cgattcacgc catagaggct tgccgtatcc
    11401 aagaaatgga atgggcgtat catttattcc aagaagcgtg cctgattgat ttaggaccag
    11461 atccgcattc atcagatgac ggaattcatg ctgcttcatt aggagcacta tggctagcag
    11521 ttatatttgg tttttcaggt attacagtaa cgaatggaac tctttctatt tctccaagac
    11581 ttccttcagc atgggaaaaa atttcttttc cttttatttg gaaaaatcac aggttgctat
    11641 ttgttttgac aaaaaaacaa atagtaataa aaaaagagac gaaagaaaca ttacctattt
    11701 ttgtcaatgg agaccaatac cagctggaaa aagaactgat cttggaacgg aggggaaaat
    11761 aatgcgtatt ctgattgcgc cagattcttt caaagaaagt ctgacagcct tagaggcagc
    11821 acaagccatt caatcaggtt ttcaagaagt ttttgttcag gctgaatatg atctgctacc
    11881 tataggcgat ggaggagaag gaacggtttc atcattagca gacagtttaa ggttgaagcg
    11941 gcaaacagca gtcgttacta atgcatttgg caaaaacaaa gaaatccagt acgcttcaga
    12001 cggtcaaact gcgatttttg aaatggcaga aatcgtcgga ttgaaagatg tcccactaaa
    12061 ccaacaacag ccacttaggt tatccacgcg aggagtagga gaactgatac gacaattgtt
    12121 gcaacaaggt gttcgacaca tcatcatcgg cgttggcgga agtagtacca acgatggcgg
    12181 catcggcatg gcagaaggtc taggttatgc tttttacgat caagataatc aaaaagtaga
    12241 agcactggga gaaaatctag gtaaaattgc tacagttgat aaaacgaacg tgcctattga
    12301 gctgattaaa gccaaaatca ctgtcattac tgatgttact aatcttttat gcggaaaaga
    12361 aggcgcttct tttgttttcg gaccacaaaa aggcttgaaa gaaaatgaat tagcagtggc
    12421 tgaccaagcc atgcatgatt tttatatgag atttgcccct caagtaatcg aggcatcggg
    12481 cagtagtgct ggtggtggaa tgggtgcagg actgttagca tttgcttcag ggaaaatagt
    12541 ctctggcatc gactacgtat tggatttact ggagttcgat aaacgggtac agcaagcaga
    12601 cctcgtaatc gtaggcgaag gacgtctaga tggacaaagt ctaaaaggaa aagcgccagt
    12661 tgggattgct agaagagtac cagagggcgt tcctgtgata gctatttgcg gaagtgttgg
    12721 taaaggaagc gaaaaagcag cagatgttgg catttcggcc gtttttccga tccttcctgc
    12781 agctgtgcct ttacaagaag ctttacagca agccactgat aatcttcatc gcacagctaa
    12841 aaacatcgca tctttacttc aagtcgttca ttttcaagga ggtaatcgat gaaaatcaaa
    12901 gcagcactat tcgatttaga tggcgttctt gtagacacag ctcgttatca ttacgaagcg
    12961 tggctggttt tagctaacca gctgtccatt ccattcactg agaaagaaaa cgagcaactg
    13021 aaaggaatat ccagaaccga gtcactagag cgattgctgt catttggaaa aatggaacag
    13081 aaattctcag aaaaagagaa atcagccttt gctgagcaaa aaaacaatct atatcttcaa
    13141 gcgattcaaa aaatggatga aacatccgtt ttgcctggtg caatagctgt tcttgagtac
    13201 ttgaaaaaaa caaacatcaa gatcggattg ggatcagcaa gcaaaaatgc acgcctcatt
    13261 ttagaaaaaa cgaatttgac aagttatttt gatgtcctta ttgacggcac gcaagtatca
    13321 aaagcaaaac ctgatccaga agtttttctc aaaggagcac agcaattgaa tgtaccgcca
    13381 aatgcatgct tggtcattga agattcagaa gctggttgtc aagcagcttt ggctggtaac
    13441 atgcatgtat tgggaatagg tgaaaacata aatcttcctt ctgctgaata tgtgattcct
    13501 gatttaaccg tttttgatca agtacgttcg ttttggcatt tatcagaagc cgtatcacgt
    13561 atataatagt ttccgttttt tctgtcacaa gcaaaacgtt atcctccttt tggaaatcaa
    13621 ggtatgatga gaaaaaaggg ggattttata tgacaaaaaa agcagttttc gcaggcggat
    13681 gcttttggtg tatggtcaaa ccatttgaag agcagccagg gatcatttct gtgacatccg
    13741 gttatactgg cgggcatgta ccaaatccaa cttatgaaca agtaacaaca ggcacaacag
    13801 ggcatacaga agcagtagag atcgaatatg atccagaagt tatttcttat gatcagttgg
    13861 tggaaatcta ttggcagcaa accgatccga ctgacgcatt aggacagttc gctgatcgcg
    13921 gtgattctta tcgtccagtg atctactata atgatgaaga agaacgaaat attgcagagc
    13981 gttcaaaaca agcattgcaa gacagcgggc gattcgataa gccaatcgtg acaaaaatcg
    14041 aaccaagaag cgaattttat cctgcagaag attaccatca ggattattac aagaaaaaca
    14101 agctccatta caatctttat cgagaaggtt ctggacgtgc aggtttcctt cgtcgacatt
    14161 ggggcgtcaa gaaataagaa gccgaaaaag ctacttatgg aacaccgtat atcagaggtt
    14221 gtaaaaccag cattttgagc taatactgag ggtcagctga ttgaacacca tttagtggag
    14281 aggttgtgaa cctgtcgcct agctccaagt cacaaggatt tggtaagaga acactatata
    14341 tcagaggttg tgacaagtcg tttgtcacaa ccttttttca atacctaaga tgaaggacaa
    14401 tcagcaacgc actgctcaaa aaaggaatga aaggcagctt ttccaacttt tttttatgac
    14461 agataaagta aacagaaaaa acggatattc caaacagact agcaagaaac aagatctgaa
    14521 gcaactcttc tagagacaag ccaccactcc aagaaagcat caacagagta tcgccagctc
    14581 cgagatagga ttgaccatag cgaaggcaca caccgcctac gataaacgtt aacagcacac
    14641 tttgccattg gaagacgccg gcctgcaacc aaaaaatcca taaaattgcc caagaacaga
    14701 atagcgtgtg agaatggacc atgaagtaaa ataaatccat caacgaaaat agcaaagccg
    14761 aaagcatcca tatgaacgtc gaaaaatttc ttgcggaaga aaaagtaaag aaccaggcaa
    14821 aaagaccacc acataaggct tctgccaaca aatagtagaa gggcaaccga cacctgcaat
    14881 agcggcagcg aaatcgttgg acgatgatcg agagaatcgg aatcaattca taccaagaaa
    14941 gcgaacgaca gcatttgaca caatgagatc taggataaat aatagagtga cctaacggaa
    15001 tccgctgggc ggttaaacat aaaaatgatc cgatacagca accaatgata aaataaagta
    15061 acacgccatc tccccctttt caaaaccaag atacgcaagc ttaaactaaa tttttgtaca
    15121 tacataaaat ttgaggtgaa aaaatgaacg tttcaatcgg attagatatc ggaaccacac
    15181 aaaccaaagc agttgctttc acagatgaag cccaagaagc agcatccgct tattttcgct
    15241 accctttgat ccaagaaaca caagggatgg cagaacaaga tcaggaattg atctttcaag
    15301 gtgtctgcac cgttataaaa gaagtaatta aacagttgcc ttcagacaca atcattcaat
    15361 ttgtctcctt ctcctctgcg atgcatggcc tgatattgtt ggatgaagag aaaacaccgt
    15421 tatctcgaat gatcacttgg gcagataacc gagcagcgga cgaagcagaa aaactgaaat
    15481 ataccgtgct tggcaaagaa ttttatcggc agactgggac gccgattcat ccaatgacac
    15541 cattgaacaa aatcaagtgg ctgcaaaaca gccaacctga attattcaaa aaaactgctt
    15601 attttgtcgg tatcaaagac tatatctttt atagattgtt tggcgaactg atcagcgact
    15661 acggaacagc ttcaggaact ggatattttg atatccatga attccaatgg gcagaagaag
    15721 tgcttgacta cttgaacatt cgaatagagc aattgccgca agtctttcct tccacctatc
    15781 aagtaacagg tctaccatca aaaacagcag cagaactagg tttgccaaat gatatatcat
    15841 ttgttttggg aggggcagac gggccgcttt ctaatctagg gttaggtgct tttggtgata
    15901 cgatcgccgc tttgaccgta ggaacgagcg gagcacttcg ttttattact actcagcctc
    15961 agttccatcc agaaatggag acgttttgtt atgttctgga tcagaaacat tgggtagtgg
    16021 gaggagcgac tagcaatggt gctggcatct ttgattgggc agggcataca tttttacagg
    16081 aagtcgttca atctgctaaa gaaaaaggag aaaatccata tgatgcactg ctttcggcta
    16141 tcgcttttgt gccagcgggt gccaatggtc tgatttttca tccctattta cttggtgagc
    16201 gagcaccgct ttgggacgca gatgcgtttg gcagttttat cggtttacag cggcaacata
    16261 cagaaaaaga aatgatgaag gcgattctag aagggatctg tctcaacctt tatcggattt
    16321 taaaaggagt ttgccaggaa aatcagctca cagagatccg tgctacaggc gggtttgctc
    16381 aatctccgat ttttcgtcag atgatggctg atgtactggg ttacccgctt gttttcccta
    16441 ctgtcacaga agcatccgca ttaggagctg tgattttagg ctggagaagc atcggaaaga
    16501 tccaaagtat cagtgaagca caggaattga tagaattgaa agagcaagtt ggagtacaag
    16561 aaactgatca taaaaaatat cagaaaatct atccattatt tgtttcgaca caaaagcatt
    16621 tagcacagac ttatcagcaa tttgctcaac tgcgagaaga attagctgat tctgaaaaat
    16681 gattggcttt acggataaaa aagcgaactg aaaatcgttc tcaggtaaat gattttgttg
    16741 tacttttcat tgaaagtcta ctattatttt aagtgtaaga aaatgttagg aggcaagaaa
    16801 gatgaaattc caacaaacca aagaagtatt aaatcaatta gttgcagatc taagccaaat
    16861 gtctgttgtt gttcaccaaa cacattggta catgagagga ccaggtttcc ttacattgca
    16921 cccaatgatg gatgagttca tggatgattt gaacgctcag ttagatgaag tatccgaacg
    16981 cttgatcaca ttagacggtt caccttattc tacattgcgt gaatttgcag ataacacaaa
    17041 aatccctgat gaaattggcc gctgggatcg tactattcca gaacgtttgg aaacacttgt
    17101 tgcaggctat cgttatctag cagatcttta ccaaaaagga atcgaagttt ctggagaaga
    17161 aggcgacgat ccaacacaag atatctttat cgaattcaaa acagccacag aaaaacgtat
    17221 ctggatgatc caagctcatt tgggcaaagc gccagagatc gacgcgtaaa aactgttgtt
    17281 gaacttgacc gaaaaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
    17341 acgtcaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
    17401 tgaacaccgt ttaccaagag gttgtaagcc tgccgttcag ctccgagtca caagaaacaa
    17461 ttttaaaaaa cagcttctta tgttttatgg atattgtgac ttgtgaccga ggagttggca
    17521 ggagaacacc gtttattcaa aataaggggt tgtgacaaga cttttgtcac agcctctttt
    17581 tgtttttcat tttgacagga aacgaccctt ttatataaaa gaaagaaaag aagtaaagtt
    17641 tcatataaat ttttcaatca agcagaaaaa cgttgacatt gactagatta gcgggtaata
    17701 tgttaaatgg tattgtttgc cccacaaaga gccccggaaa ctcgaagtgt atgtcaaaca
    17761 tcagaactta aattggagga aaataacgtg cgtacaacat atatggccaa accaggcgaa
    17821 gtagaacgta aatggtatgt agtagacgca actgatgttc cattgggacg tctttcaaca
    17881 gttgttgcat ctgtactacg tggtaaaaat aaaccaactt tcacacctca tttggatact
    17941 ggcgatttcg tcatcgtcat caatgcagac aaagtgaaat taacaggtaa aaaagcgact
    18001 gacaaaattt attaccgtca cagcaactac ccaggaggat tgaaatcaat cactgctggc
    18061 gaattgcgtg ctaaaaattc tcgtcgtttg atcgaaactt ctgtaaaagg tatgcttcct
    18121 aaaaacactt taggccgcaa acaatttaca aaattgaacg tatacggtgg agcagaacat
    18181 ccacatgcag cacaacaacc agaagtttta gacatcacaa acttaatcta ataggaggga
    18241 atttcattgg cacaagttca atatatcggc acaggccgtc gtaaaaacgc agttgctcgc
    18301 gtacgtctag taccaggaac tggcaaaatt actgtaaata aaaaagatgt tgaagaatac
    18361 attccacatg ctgacttgcg tgaagtaatc aatcaacctt tcgctgttac tgaaacaaaa
    18421 ggcgcatatg acgtatttgt taacgtaaac ggcggaggat acgctggaca atcaggagct
    18481 atccgtcatg gtatcgctcg tgcattgcta gaagttgatc ctgacttccg tttagctctt
    18541 aaacgtgctg gcttacttac tcgtgacgca cgtatggtcg aacgtaaaaa accaggtctt
    18601 aagaaagcac gtaaagcttc acaattctca aaacgttaat atcctttata tatcaacgtt
    18661 tctggcactt ttcaattatt tggaaagtgc ttcttttgtt tttaagaaca catttaagaa
    18721 cacatatcaa tcacacttat tcaatcctct ctgaaaagct tcaaaaacag ccttatcaac
    18781 tatatctggt gtgttcacat atatctttgt tgtagaaata tctgagtgtg ttaaagcatt
    18841 tgaaatttga ttcatatctg ctccaccttg tctagctata gttgcataag tatgacggag
    18901 tttgtgtgga gataaatgaa ctaagtcagg atgccttctt ttaattgaat tgattcgata
    18961 gtttaggtaa tcggcatgaa caggacaatt tacttctcca gatggtttag tataagtaaa
    19021 gagatactga tctaacgttt gcttaatttt taatttcaac aattgatcag cttgtaccga
    19081 tttccattca cttagcaatg tcattacaac ttctggtact tgaatgacgg tatcttttcg
    19141 accttttgta aacttttttc tttgatattt atctaaagta tgtttaagac ggaccgtttg
    19201 tttttcaaaa tcaatatatt tccattgcaa agcgtaagtc tcactttttc tatcaccaag
    19261 atacagggta atcataaaaa gtgtgtaatc atgaaaagta agtaaatgat tattaaaatc
    19321 atcatttact gcttctatcc aattagtaag ttcttttgat gatagtgctt gcttaccttc
    19381 ttgtattctt ttttcttcta aagcattttt tttaggtgca gtgatacttt gaataatttt
    19441 agtagttcga ttaaattcga tataatttag tatttctgca atatcgaaca tggaacgaac
    19501 atatcctttt atgattttta tatttgcata ttctttcgat tttttggtta attcatctga
    19561 tattatctca gtattgatat ttagataatt cattgcgtat tcaccaaaca taggtaaaat
    19621 atgcaaccga aatatatctt ttgtattact aatggttata tcactaggag gcctgtttga
    19681 tctaattgtt tgacctaatt catatctagg aagccattta gattcgtaaa attttttaaa
    19741 cgttattttt ccttttaatt ctaatgagtt tgcattctcc tctctcaaaa ctttttctat
    19801 tttcttcttc atatcatttt cggctttaat cgccaattgc ctatttggaa atgttttttg
    19861 atacttttct gatgcaattc ctgttatttc tttaaacctt tttggatatc tcagattagt
    19921 tttatatttc ccatttgaaa gctttgtagg caaaatcctc actccttaaa aaaagtaagg
    19981 cagtatattt cactaatttt agtatatgcg gcgagaagaa aatttcaagt tattttaaat
    20041 gtattcataa agtctttaat atcttctacg tcaaagcgaa ctacaccacc aactcttatt
    20101 actgggaaat tatatatacg aatccactta tctagagtat tattatcgat tcctagaaat
    20161 gcacaggttt gttttttatt caggtagact ctggattcta taaaatcatc tcctaaaata
    20221 gtagttatga aatttttgaa ttgctttggg taagcacata ggaatttcac ctctccccag
    20281 tttacggcga gccttacgga agtatcatta tttgctgcta ttgtcatggc attttgtttt
    20341 cccagaacaa aaagaaatct ctaagtattt gtcgctaatc aggtcatggc gtacttgagt
    20401 ttctagctcg aatagcaggg atcgttattc aaaaattgta tttaattgtc aatgatcaaa
    20461 aatagttttt ttcctattca ctattaataa cgataaacat gtataaaaag acacactacg
    20521 aaaaaagtta aatggagttt actgattttc atatttgtat atggaacaga catagtcttg
    20581 aataattttt ataaatgttt tgatgcttat caacttattc aacccgctat ccacttcaag
    20641 tataattttt ccatacgcca ataaagtagt tttaggagca tgttctcatt tgagaacgct
    20701 ccttttttat atattttgaa agctacttac tatacgcgta gtttcaaata gttttgtcat
    20761 tctaatcttc agcaacagaa aaaaatctaa actataaaat ttagggttga ctaaaatata
    20821 atttttgtat accatatgat cgtgataatc attatcattt agaaggagaa agttatgtta
    20881 gaagtgaaaa agcttaatgt ttgttataac gattttttag cattgaacga gatttcttta
    20941 aaaatacaag aaggtgcttt aacaggtatt attggtccaa atggtgctgg aaaatccaca
    21001 ttgcttaaag caatgttaaa tattatccct catcagggag aggtctccat taataatgaa
    21061 gatatcaata aaaaactggt gaagattgca tatgtagagc aaaaagctga tattgatttt
    21121 acttttccaa taaaagtaaa agaatgtgta tctatgggaa catatgccgg aatgaaagtt
    21181 tttcaaagaa ttaaaaatgc agaatggcaa agagtttcaa aggctttgga aaaggtcgat
    21241 atgggaaaat attctaatca tcaaatagga gaattatctg gaggacaatt tcaacgtgta
    21301 cttttagcaa gatgtcttgc tcaaaatgcg gatttcatat ttttagatga accatttgta
    21361 gggatagact tagttagtga acgaataatt atggatacgt taaaaaaatt gaaacatcag
    21421 ggaaaaacag tgctaattgt gcatcatgat ttaagcaagg taaagaagta ttttgataat
    21481 attattcttt tgaatcgaaa tctaattgct cacggaagtg tagaatctgt atttaatgaa
    21541 gaaaatttaa aaaaagccta tggagatact atatttattg gagaggggga ataatatgct
    21601 tcaaaatttt attaatggtc tgtatgactt tcacttttta cagaatgctc taattacatc
    21661 tgtagttata gggattgttt ctggaacagt aggttgtttt attattttaa gaggaatgtc
    21721 tcttatgggt gatgctattt ctcatgctgt tttgccagga gtagcaattt cttatatttt
    21781 aggtattaat ttttttattg gtgcgattgt atttggtcta ttagcttcta tcattattac
    21841 ttttataaaa agcaatagtg taataaaagg agatacagca attggtatta ctttcagttc
    21901 ttttttagca ctaggagtta tccttatagg agttgcaaat agctcaacgg atttatttca
    21961 tattttattt ggaaatattt tagctgtaca agatattgat aagtggatta caattggcgt
    22021 ttcggtttta gtgttggtta tagtagttct attttttaaa gaattattaa tcacttcttt
    22081 tgatccatta atggcaaaag caatagggat gaatgtcagt ttttatcatt atctattgat
    22141 ggttctatta actttagtag cagtaacagc tatgcaaagt gtgggaacta tattaattgt
    22201 tgcaatgtta attacaccag cagctacagc atatttatat gcaaatagct tgaaaactat
    22261 gatatttatt tctgcaacag tcggagcaag ttcatcattg cttggattgt ttattggtta
    22321 tagttttaac atagcagcag gatcaagtat tgttttaaca gcggcattct tatttgtaat
    22381 tagttttcta atctcaccaa aacaaaaatt tttaagaaag aaggagagat cgttatgatg
    22441 agaaaatgga aagcagtact gggaagtctg gggattttga ttgctctttt tatattcggg
    22501 gcatgttcaa taaatagtaa agacaaagat aaagtggctt cgaacgaaaa attaaaagta
    22561 gtagttacta attcgatttt agcagatatt accgaaaata tagcaaaaga taaaattgat
    22621 ttacacagta tcgtacctat tgggaaagat ccccacgaat atgaaccttt gcctgaagat
    22681 gttcaaaaaa cttcaaaagc agatttgatt ttttataacg gtgttaactt ggaaactgga
    22741 ggaaatgctt ggtttacaaa attagttaaa aatgcgaaca aagaggaaaa caaagactat
    22801 tttgcagcaa gtgatggcat agatgttatt tacttagagg gtcagagtga gaaagggaag
    22861 gaagatcctc atgcttggtt aaatttagaa aacggtatta tttacgctaa aaatattgaa
    22921 aaacagttag cggaaaaaga tcctgataat aaaaaattct ataaagaaaa tctagataag
    22981 tatattgaaa aattggattc tctagacaaa gaagctaaat ctaaatttgc ttcaattccg
    23041 aatgataaaa aaatgattgt tacaagtgaa ggatgcttta aatatttctc gaaagcgtat
    23101 aatgtgcctt ctgcttacat ttgggaaatc aacactgaag aagaaggaac accagatcaa
    23161 ataaaacact tagttgaaaa attacgcaca acaaaagttc cctccttatt cgtagaaagc
    23221 agtgtggatg atagaccaat gaaaacagta tcaaaagata ccaatattcc tatctattca
    23281 acgattttta ctgattcaat tgcagaaaaa ggacaagatg gtgatagtta ctatgcgatg
    23341 atgaaatgga acctggataa aattgctgaa ggcctttcga aataatttgg agggtaggta
    23401 tggtacaaat taaaacgact actatactaa tttatttact agcaattcag gaattatcaa
    23461 aaagaaaaaa gaaattaaaa aataatgatt tagcaaaagt tctaagtgtt agtccagcat
    23521 ctgtaagtga gatgctggct aaacttagat atgaggacta tttagatata ggatttcaat
    23581 taactgataa agggaaaaaa tttattgacg attacaaaaa aggttttaag taaaagactt
    23641 tatttttagg gaggataaga aaatggctat tggagtgatt atgataggac ttatcctaat
    23701 agctggatat aattttttta aatctttctc ggattatttt agaaaaggtg aatgatttag
    23761 atgagtctat taattcttgt aggattgctt tttgtatcag ttattctgct ctttctcatt
    23821 agtttagctc taaggaatga gttcagtaat aaacgttaac gattgaaact tcaagtcctg
    23881 tgtaagataa taaagttaat tcatcttatt aggaggaagt acccatagaa accagagatt
    23941 atattataac tcaccaaaag ttaatagaaa gtggactaag ccattttgaa aaagaaggat
    24001 ttcagcaaac taagataagg caactgtgcc tgaccgcagg cgtaactacg ggtgcttttt
    24061 ataaacattt tacttctaag gaagcgttat ttgaagaaat tattcagccg catattaatg
    24121 cgcttattaa tatgtataat gaagagttta ccaaatacct cttaaactta gaaaatatag
    24181 atatagagaa aagttggaga gagaacttca atgaattaga tccatttatt aattatattt
    24241 ttgagaataa acgtatattc gatttgattt tattccaatc agctggaacc aaatatcaag
    24301 atattgttga aaaaattaca tcatttgtga cgaaaaagac agtctgttgt ctagataagt
    24361 taaagactat cggaatgatc aatcaaaatt tagtctttaa tgaagaagaa atccacttct
    24421 acatttactc attctacgct acattctatg atatattgaa gcatagctat tcaaaagaaa
    24481 agactatgct attaaccaga agattgtatc aattttctgt tcctgggtgg attgaattac
    24541 tgagttaatt aaaaaaagct tgcattgaga attgttctca gttatgcttt ttgataagat
    24601 cgcactgtta tctgaataac ggtgcatttt tatcagctta agataacagt gcgatcttat
    24661 tttttggagg tgaaagagtg tcaaaaa
//
LOCUS       DAZAFF010000037        24182 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000037 DAZAFF010000000
VERSION     DAZAFF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.037"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACG9BB_001417"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACG9BB_001417"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFN2837362.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACG9BB_001418"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACG9BB_001418"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFN2837363.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACG9BB_001419"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACG9BB_001419"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFN2837364.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="ACG9BB_001420"
     CDS             complement(3373..4680)
                     /locus_tag="ACG9BB_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFN2837365.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="ACG9BB_001421"
     CDS             complement(4683..5264)
                     /locus_tag="ACG9BB_001421"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFN2837366.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="ACG9BB_001422"
     CDS             complement(5836..7128)
                     /locus_tag="ACG9BB_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN2837367.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="ACG9BB_001423"
     CDS             complement(7125..7748)
                     /locus_tag="ACG9BB_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837368.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="ACG9BB_001424"
     CDS             complement(7751..8587)
                     /locus_tag="ACG9BB_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2837369.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="ACG9BB_001425"
     CDS             complement(8604..9491)
                     /locus_tag="ACG9BB_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2837370.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="ACG9BB_001426"
     CDS             complement(9507..10790)
                     /locus_tag="ACG9BB_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2837371.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="ACG9BB_001427"
     CDS             complement(11473..12156)
                     /locus_tag="ACG9BB_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFN2837372.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="ACG9BB_001428"
     CDS             complement(12174..12791)
                     /locus_tag="ACG9BB_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN2837373.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="ACG9BB_001429"
     CDS             complement(12801..13625)
                     /locus_tag="ACG9BB_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFN2837374.1"
                     /translation="MNKFLGFRAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="ACG9BB_001430"
     CDS             complement(13618..14178)
                     /locus_tag="ACG9BB_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837375.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="ACG9BB_001431"
     CDS             14352..15308
                     /locus_tag="ACG9BB_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN2837376.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="ACG9BB_001432"
     CDS             complement(15613..16569)
                     /locus_tag="ACG9BB_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFN2837377.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="ACG9BB_001433"
     CDS             16673..17275
                     /locus_tag="ACG9BB_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN2837378.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="ACG9BB_001434"
     CDS             complement(17363..18862)
                     /locus_tag="ACG9BB_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFN2837379.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="ACG9BB_001435"
     CDS             complement(18878..19402)
                     /locus_tag="ACG9BB_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFN2837380.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="ACG9BB_001436"
     CDS             19700..20449
                     /locus_tag="ACG9BB_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFN2837381.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="ACG9BB_001437"
     CDS             20440..21225
                     /locus_tag="ACG9BB_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN2837382.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="ACG9BB_001438"
     CDS             21194..21910
                     /locus_tag="ACG9BB_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN2837383.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="ACG9BB_001439"
     CDS             complement(22012..23031)
                     /locus_tag="ACG9BB_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFN2837384.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24051)
                     /locus_tag="ACG9BB_001440"
     CDS             complement(23050..24051)
                     /locus_tag="ACG9BB_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFN2837385.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7159 a         5215 c         4216 g         7592 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctctaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tt
//
LOCUS       DAZAFF010000038        23864 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000038 DAZAFF010000000
VERSION     DAZAFF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.038"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..1013
                     /locus_tag="ACG9BB_001441"
     CDS             144..1013
                     /locus_tag="ACG9BB_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN2837386.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1222..2247
                     /locus_tag="ACG9BB_001442"
     CDS             1222..2247
                     /locus_tag="ACG9BB_001442"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFN2837387.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2286..3236
                     /gene="manA"
                     /locus_tag="ACG9BB_001443"
     CDS             2286..3236
                     /gene="manA"
                     /locus_tag="ACG9BB_001443"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFN2837388.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3330..5033
                     /locus_tag="ACG9BB_001444"
     CDS             3330..5033
                     /locus_tag="ACG9BB_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN2837389.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5035..7068
                     /locus_tag="ACG9BB_001445"
     CDS             5035..7068
                     /locus_tag="ACG9BB_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFN2837390.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7079..7519
                     /locus_tag="ACG9BB_001446"
     CDS             7079..7519
                     /locus_tag="ACG9BB_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2837391.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7719..8846)
                     /locus_tag="ACG9BB_001447"
     CDS             complement(7719..8846)
                     /locus_tag="ACG9BB_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2837392.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8849..9526)
                     /locus_tag="ACG9BB_001448"
     CDS             complement(8849..9526)
                     /locus_tag="ACG9BB_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFN2837393.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9790..11556
                     /locus_tag="ACG9BB_001449"
     CDS             9790..11556
                     /locus_tag="ACG9BB_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFN2837394.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11571..12008
                     /locus_tag="ACG9BB_001450"
     CDS             11571..12008
                     /locus_tag="ACG9BB_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2837395.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12021..13172
                     /locus_tag="ACG9BB_001451"
     CDS             12021..13172
                     /locus_tag="ACG9BB_001451"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFN2837396.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13234..13932)
                     /locus_tag="ACG9BB_001452"
     CDS             complement(13234..13932)
                     /locus_tag="ACG9BB_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFN2837397.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13925..15277)
                     /locus_tag="ACG9BB_001453"
     CDS             complement(13925..15277)
                     /locus_tag="ACG9BB_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFN2837398.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15500..16900)
                     /locus_tag="ACG9BB_001454"
     CDS             complement(15500..16900)
                     /locus_tag="ACG9BB_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFN2837399.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17061..17798
                     /locus_tag="ACG9BB_001455"
     CDS             17061..17798
                     /locus_tag="ACG9BB_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFN2837400.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17923..18402
                     /locus_tag="ACG9BB_001456"
     CDS             17923..18402
                     /locus_tag="ACG9BB_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFN2837401.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18399..19166
                     /locus_tag="ACG9BB_001457"
     CDS             18399..19166
                     /locus_tag="ACG9BB_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFN2837402.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19396..19965
                     /gene="lepB"
                     /locus_tag="ACG9BB_001458"
     CDS             19396..19965
                     /gene="lepB"
                     /locus_tag="ACG9BB_001458"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFN2837403.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20069..20671)
                     /locus_tag="ACG9BB_001459"
     CDS             complement(20069..20671)
                     /locus_tag="ACG9BB_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFN2837404.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20777..21007
                     /locus_tag="ACG9BB_001460"
     CDS             20777..21007
                     /locus_tag="ACG9BB_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837405.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            20998..21246
                     /locus_tag="ACG9BB_001461"
     CDS             20998..21246
                     /locus_tag="ACG9BB_001461"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2837406.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21352..21936
                     /locus_tag="ACG9BB_001462"
     CDS             21352..21936
                     /locus_tag="ACG9BB_001462"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFN2837407.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21939..23012
                     /gene="prfA"
                     /locus_tag="ACG9BB_001463"
     CDS             21939..23012
                     /gene="prfA"
                     /locus_tag="ACG9BB_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFN2837408.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23005..23844
                     /gene="prmC"
                     /locus_tag="ACG9BB_001464"
     CDS             23005..23844
                     /gene="prmC"
                     /locus_tag="ACG9BB_001464"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFN2837409.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
BASE COUNT         8141 a         4209 c         4832 g         6682 t
ORIGIN      
        1 ataagagttt ttactcatta atttacaaaa aacaagcaaa atgcgaattt tctcacattt
       61 tttttaacaa aatcaaagtt attgcttgaa agaagaagaa tccatcgtta aaataaagct
      121 tgacgtagag gggagcaaga aatatgaaag attttcaatt gggggctgcc acctatttaa
      181 aaagtgtagc cattcgggtg aaagaccgag ataaaatgat tgatttttac aagaacgttg
      241 ttggctttga tttgaaaaga gaagaaaacg aattagccat tttaggaaca gaagaagctg
      301 gaagtgaaat gttgctgctg gaagaaagtc ctagagcgaa tgatcacatg ggggtgatca
      361 aaaagctgaa ccgtttttca ttgattatcc ctagcacaga agaaatggca gatatccttt
      421 gtcgtatccg taaaatgaat tatccaatca aagaagcttt agaagatcat ggacgtatgg
      481 ggattttgct cgtagaccca gaagagaatg aattggaaat ttaccatgaa gagaataaga
      541 acgaccaaag agaaattcca gaaacaatgg atcaagaagc attattaaga aaatctgaag
      601 aaaaattcag taaactatca acaggttctt attttgaaaa aatccattta aatgttacta
      661 acttaacaaa agaacgccaa tttcttaatc atgtacttgg tttgaaggta cataatgaag
      721 aagagggcat ccatgtattg aatgaaggag atttccatgt tggtttgaca gaagctagcg
      781 gaggcgcaat tgatttgcct acagatgaag tgattggact tgattttctg aaattcatga
      841 tcgatactga aagcatggaa ggactgattg ctcacttgca gcaactatca gttgatttct
      901 tcttagacaa aaagcacaca gtactcacaa tctatgatgc tataggaatc gaatggtggt
      961 tcgtcagtga gccaaaagaa aagaataaaa ctgacattcg atgcgacgct taactttttc
     1021 tttcttaacg attgtcgcat gtctcgtcat tttatgaacg gtcatactat cgcttattct
     1081 agagatagaa agagaaaata gtataccggg ggaaactgcg cggtttttcg cgcagttttt
     1141 ttgtgtaaaa aaggttgatt cctcatagat taagctttaa gcttggactg taagaatctg
     1201 atagaaaagc aggagaaaaa gatgtatcca cacacgaaac agcttatttt gaaaaagatg
     1261 gaagaaggat tttatccagg tgtagcagtc gccttcataa aagaaaatca agtagaaaca
     1321 tttacagccg gctttgccca agtcgttcct gaaaagcagc tgatgacaga agatctgttg
     1381 tttgatgcag cctcactgac gaaagtcgtt gcaaccactt cattagtttt acgtctatta
     1441 gaagatggaa agatagaact cgatcaaccg cttcataaat atcttcctga atttgaaaat
     1501 cagacaatca ctttgcgtca tttactgaca catacttctg atatcaaaac atggatacca
     1561 aacagagata agctatctca ggaagaattg atcgccgctt atttgaagct agaaccaggt
     1621 gagcaactag gaaaagaagt aaaatataca gatgccggta cgattcttct tgggttgatg
     1681 ctagaggaaa tttaccaaga aactgcagtg gaaatctttc aagaacaagt attggaacca
     1741 ttaggaatga tgaacagctt atttcttcct tttccttcca aaaaaatcgt tcccacagaa
     1801 caactgcctt cgggtgaggt tttgcgtgga caaactcatg atccgaaagc aagagttttg
     1861 gctgaacatg caggtaacgc gggtctcttt attgatttga aagacagcat tacttttgtc
     1921 caaatgtatt tgaacaaggg aaaaatccgt aatggtcgtt ttttagaaga agagacgatt
     1981 gattctttac tgcaagatca aacaccgaaa aaaaatggct atcgttcttt aggatgggac
     2041 ttaaaaaagg atgtagaaga cggtcatgcg attctttttc atactggtta tacaggtacg
     2101 tttttcttga ttgatataaa aacacaagaa gcgttgatat ttttgtcaaa tcgcgtacat
     2161 cctgtcgatc atcgtgcgga gtatctcata tgtcgagatg aaattgttga aaactttttg
     2221 aaagagaaag cggtattacc gaaataatgc tataatagct atgaaaaatg agaggagttt
     2281 gaattatgca ggaacctatg tttttaaaac cagtttttca agaaaaaatt tggggaggaa
     2341 gtcgtttacg ttcagtcttt ggttttgata tccctaacga taaaatcgga gaagattggg
     2401 cgatcagtgc gcatcctcat ggtgtcagtg tagtagaaaa tggcgaattc aaagggaaaa
     2461 gattagatga actatggtca gaacacaagg aattgtttgg ccatccttca gaaccagtgt
     2521 ttccattatt gatcaagatc ttagacgcag aagatgatct atctgtacaa gtacatccag
     2581 atgatgccta tgggatgaaa catgaaggtg aattaggaaa aactgaatgc tggtatatca
     2641 tcgatgctga acctggtgca gaaatcatct atggtcatca tgcaaagaca agagaagaac
     2701 tagctgagat gatcaaagat gggcgctggg atgacctttt gaagaaagta ccagttaaaa
     2761 aaggtgattt cttttatgta ccaagcggaa cgatccatgc tattggtaaa ggaatcatga
     2821 ttttagaaac acagcaaagt tctgacacga cttaccgcgt gtatgactac gacagaaaag
     2881 atgcgcaagg acaaacaaga gagctgcata tccaacaatc gatcgatgta acaactgtcc
     2941 ctgcgaaaac accagaatta caaatcaaag aagtccgaaa agggaattca tcgatcgtca
     3001 cttatttaga aactgagttt ttcaatgtat atgagtggga tattaaagga atcacatctt
     3061 tcaaaaaaca agccccttat acattgatga cggtaatcga aggagcagga gaccttgtcg
     3121 ttgacggaaa aacatatcca ttagaaaaag gaacaagctg tattattcca aatggcgttt
     3181 ctgagtgggc ggtccaaggt gaactagcaa tcattgcgtc tgaaccaggc aaatagttcc
     3241 cagatagctg aaagcagcag acggtttttc gtttgctgct ttttctacat aaaatccgtc
     3301 catcaataaa tagaagtctg gtgaaaaaga tggaatacaa atggaaagca aacaacagca
     3361 gtcgcgtgtc tatgaaacaa ctgagaaaag ctggacatca cttaaatgaa atgatcatgc
     3421 caaatctaag tctgtttatc gcttggggaa tcttgtcatt agcaacaaaa ggtgtgtctg
     3481 gcgatttgca caccacatta gaagaggtat gccattggat gatccagctg cttttaccca
     3541 tcttgatcag ttattcaggc gggaaatcgg tagggggaca aaaaggggca gtcgccggtg
     3601 caattgcttc tttagggttg atcgctcgtt ccaatgctcc acaaatcgta ggagcgatga
     3661 ttatcgggcc gcttgccggc atcgtatatg aacaatttgt ttctagattc catgtgaaat
     3721 tcaaggctgg atatgaaatg cttgtcagca acctactagt aggactgact ggtagcgtta
     3781 tttgtataat tggaatcgtt tttattgaac cagttttaaa tagtgttcac ttgttgctcg
     3841 cctccgttgt gtcttggcta gtcacaatga atctgttacc acttgtcagt ctgatcttag
     3901 aacctctaaa agtactattt ttcaataatg caatcaatca tggtgtattg acacctttag
     3961 gaatcgaatt ctcacaaaca gtcgggtact cttccttatt tttgatggag gcaaatcctg
     4021 gacctggttt agggatctta ctttctatcc tttgttttgc agaaaaacag gaaaaagtaa
     4081 atgcgagtgg agcattgatg atccatgcga ttggcgggat tcacgaaatc tattttccat
     4141 ttgtcttgct gcgaccgtat ctatttttag ctgtaatggc tggtggggca agcggcacgt
     4201 tgatatttca atggttagat acgggattag ctgcacctgt atcgcctggg tcactactcg
     4261 tgatttttgc caatgcgcct tatccacaac tagctggggt aagtataggg atagcaatca
     4321 gtacatttgt gaccttcatt tgtgcagcga tactattgaa attaccaaat aaagaaaaag
     4381 agaacaaaac ggtcaaagaa aggggagaaa tcttggaacc atctatcatt cattccgtca
     4441 tttttgcttg cgactcaggt atgggatcaa gtgctatggg agccgccctg ctgaaaaaga
     4501 aattagcagc agaaaaaatc gatctttctg tcggatacag ctctatctat gaattgaata
     4561 accaagtaaa tcagctggtt atcgtgcaac atgaactagc gggtcttgca agagaaaaag
     4621 tacctcaaag tcaaatctat gtactggatc actttttgga aacagatatc ttgctgtcac
     4681 gaataaaaga agcgatccaa tttccaaagc gggaagaaaa aaaagagata gcagatatca
     4741 cagaacaaaa aacgtcgcct gctaatattg tatttttgta taaaaacaac agacgcggct
     4801 cacaaactat gggcatgacg attttaagtc aattagcaaa aaaagcgaaa aaacagtttg
     4861 ttatctcaaa agaatcaata gaaaaaatca aaatccaaga agaaacgatt tatatcgcac
     4921 aaagagaact agctgtaact gaacagctag aagaaaagat ccctcggcta gttctagttg
     4981 aacattgggt agctacgaat gaatacgaaa aactcgtgaa gggagaaata taagatgtat
     5041 ttatcgccca gagaaaaaca attattagca gaatttctca gtagcccgtc acctgtatct
     5101 atccaaaaaa tgatgaattt attgaaggtc agtaaacgga ctatttaccg cgaattagat
     5161 aatcttgaag tctcattgaa atcggtaaat gcttctttga aaaaagcagc tagaggctca
     5221 tttgtcattg aggcatcaga agaagtatta gagcaattaa aagaaaatac agatgggaaa
     5281 agtaatgact tatccactgc taaacgacaa cacgcgattt tagccgagtt gttactgacg
     5341 acagagccgt tgagtcttgc tcatttcatg gaatcatatc gcatcagtaa taccactttt
     5401 tatgcagata tcaaacagtt ggaagaaagt attcgccaac tgcctttgga aatcattcgg
     5461 aaccagggat acgagatcga aggaccggaa aaataccgcc ggcttctgac agcgaatgta
     5521 ttagaattag aaatcaatga atatgaactt tttcatagta tttctttcga tgcatcactg
     5581 aattattttt ttcgattcgt tgatccgcaa catctatctc ttgcacgcaa agtaatcggg
     5641 gaagaattga tccaaaaaaa gaccaatcta agtgatagaa agcttgaaca tttggttttg
     5701 atgctgacat tgacgatgga gcgtgtgacg aaaaatcatt tactgacaaa tgagacatat
     5761 aatagtctgg caaacaaaga attactacag acagctaaac gattgttttc acggatcgct
     5821 tctgagacaa aacaacttta tccagtaaat gagatcgtct tttttgcagg attattaaat
     5881 gacttcacga actcatttga tcagaatttc tttgaagaga cgttcgatac acagcttgct
     5941 tatttagtaa aacaactgat tcaagaagta agtgaagaga cgaatgtcca tttttatgaa
     6001 gatgaaactt tgtacaaaat gctgttgact catttatctg gtgtgttttc aagagccgtt
     6061 ttacaagaag agacactgac caatccgatt ttagaaaaaa tcatggttca atacgaggaa
     6121 gttgcgaatg caatccgtaa agcagtagag cggatttttc aagaaaaaaa attatccgaa
     6181 gaagagatcg cttatatggt ccttcatttt gcgaattcat tggaacgaag tccgaaagaa
     6241 agcaatatca gtattgcagg attttcgcca agtggattag cttctactag catgttggaa
     6301 ctacgattga aaaggtattt tcccttcatc caacaaattc atttctatcg gattgctgat
     6361 ttaaaaaaaa tcgatttaca agaagaatat gatgttgtta tttctacatc tattttacca
     6421 ggatacggtg gaaaatatat tttagtctcc ccgctactgc tagaagatga agtcaagcag
     6481 ctaaaagaag ctttttcgca tattgagaag aaaaaaaggc aaaaaattac gtataacagc
     6541 aataacgagt ggatgataga agatacttat gaggaaacaa tgcatttcat ggatcaaatc
     6601 aatcaattac tcggtcgttt ttttatccaa gaactggata atcctgagac gatttccgaa
     6661 acgattcgca gcttatttcc ttattttcct gaaggactag taacggatga agaggtggtt
     6721 catcgccggc tgcttaagcg atatgaacaa gcacctatcg gtattcctca tacaaatatg
     6781 gggttattcc atacatccag ctcattgatt tctaaaccga tcttttgtat tttcaatttg
     6841 aaacatccgt tgaccattga agggatggat agacagccag tcgatttgtc ccgtatgtta
     6901 gttatgctgg cgccttcgcc aatccaagaa aaagacggga ttcttttagg aaagatcagt
     6961 ggagcaatca tcatgaatga cttgaacacc gagatcttcc gctcaggaaa taaagaaatc
     7021 gtttatcagt tgttagcgaa aatcctgatt gaagaaataa agaaatgaag gtgaaaagat
     7081 gatacagcta ccagcagaaa aaatattgct cgatcaatcc tatcaaaatt ggaaagaagc
     7141 gttgaccgag atcggccagt ggatgaccca acaaggggga atcaaaccgg catatatcca
     7201 atcaatgatc gaaagacaag aaaaagcgag tgtctatatc gggaatttcg tagccttgcc
     7261 acatgggact gacaaagatg aagaagtagt agaagaagga atctatttag tccaagtgcc
     7321 tgatggtgtg gactttggaa caaaagagga accaaaaatc gcgacactct tatttgtagt
     7381 ggctttaaaa aaagaacatc agttagcggt gctccaagat ttagcctttt tctgttcaga
     7441 catcgaccaa gtaatggcgc tcagtgatgc ggaaaccatt gaagaagtcc aaatgatttt
     7501 tgaacatgga catatataac caatgttcac agtaaaaaaa gctgccttgc atcaagtaat
     7561 aacttcaacc aagataaaaa taagaggagc tatttttgtt gggaggttct tattactgcg
     7621 gatgcaagct tttgaaaact atttattcta gaaaaccgtt tatttgaaat aggggacggt
     7681 gacaaggttt ttgtcaccgc ccctattatt tttgtgtttt aatagctctt ttttatatag
     7741 atagtgccca tacctatgaa taataaaacg atactccata taccaagtgt caagatacca
     7801 ttcatgaaag taacctgttc tgaagctagt ggcgagaaaa tatctcctcc tatgatcaca
     7861 ttttgaaaat caacataaga agttggcaag taatgactga ttgattctat ccatccaatc
     7921 gaagaaaaca agcgatactc tgctaagaaa agacaaagaa gtgtacctaa aagattgact
     7981 agagaatgtt caaataaagc agatagaaga aaactgattg tacttaaaaa gagcacccac
     8041 aacaacagga acagaaagct ctggaaaaga tactcataca tcggcaggat tcgtatctcc
     8101 tgaccaatga tcgttcctac aggataacgg aagtctccca aaccattttt gattattact
     8161 agtatagaga cgatcaaaaa tggcatcacg agacttagca tagctgcacc ataataaatg
     8221 gtaaaacgga tgctatgttt ctttaccata ctatccagta atagattgac tagagagatt
     8281 acctgctttc ttttttcata agagatgatg gaagaaagaa acaaggcggt tatacataaa
     8341 atcaaaaaac tagaaatcat tcctgaaaaa atattttcgt agtaatttgc taaagagaga
     8401 tgcgctggca agtcaaaaat agaatatctt tggatctgat gttctttcat ataacggagc
     8461 aactcgacac gtttttgttg ttcaatgatt ggaattcctt gtctttgacc agaaatcagt
     8521 cgatccagta tatctttttc ataatgataa gttgcttcga ccatttgctg gacattccct
     8581 gtgttttttg catgtaagat tgcttcgatc agtttgttgc ttttctctaa actttccaca
     8641 aattctttct cattctcatc ttctttcaac gtttcaatca aacgttgcat ctcttcatag
     8701 ttatgattca gttcatcgaa ttctgtataa gcaagtttat gatcaaaaaa aagtgggaat
     8761 gcaaagagca cgaccaacaa tagaggaaat aaaaagttta ctttgttttt tagtaagaga
     8821 ttgcgttcaa atgtaagtaa agacatagtt acacctcata taatttacga taaatagcta
     8881 aaatctcttc catatttgag gattctgtga cgatttctcc attttttaga aaacatgctt
     8941 tagaacaaat cgattggata ttcgcgatag cgtgggaaga aagaagaatc gtcttcccct
     9001 ctttctgcca ttgtctgatg aggctattca agatatcaat acttcctggg tctaatccgt
     9061 tcaacggttc atcgaataac atgacagatg aatcactgat caagtaagca gcaagcaaga
     9121 catgttgttt cattcctaga gacatttttc caatcggcaa agtaaggtac tcagtcatct
     9181 tcaatcgttg gaaaacagtt tggcattcta ctttgctctg ccaatagtat ttgatcaaag
     9241 tcaaataatc aacagccgat agatagtcgg ataactgatt cgttgattct agaaaaaaga
     9301 aagaccggcg ataatctgta taagcagtag gagagatgtt gtgaagggac aattgatcat
     9361 aagttgttgg cagcagttgg gcaatgccat tcagcaaagt tgttttgcca aatccgttcg
     9421 gggcaatcaa agcagctact tctcctgatt ctactaaaag attcaattgt cgaatcacac
     9481 gttttttacc acgtttcatg ttcatgtttt tgatcagtaa tgtcatagat aggcctccaa
     9541 tataaaattt aataacataa tagaatggaa aacatctaaa ttaaatgcta acaaaagtta
     9601 ttgtttgcat attataaaaa aattaaaata acagttttat ttaaaattga agttttctga
     9661 taccgaaatc tttttatttt gctgacatac tcaaaccttt ggcacaaagt ttcgtgccaa
     9721 agtacgacta tttctctttt tagtaagcgg atacaattag agtatcaaaa ggagagaggg
     9781 agagacaaaa tggaacggac gattcccaaa aacaacattt cgctaaaagc gagggtacaa
     9841 aaattaggca gtactttatc aagcatggtc atgccaaata ttggagcact gattgcctgg
     9901 ggtgtattaa cggctctatt tatcccagat ggttatttac caaatgaggc tttcgctacc
     9961 atggtaagcc caatgctaac ttatctgatt cctttattga tcggttatac tggcggaaaa
    10021 gtaattgctg gtgaccgcgg atcagttgtt ggggcaattg caacgatggg tgtgattgtc
    10081 ggaacagata ttccaatgat gctaggcgcg atgatcatgg gacctcttgg tggatatgcc
    10141 atcaagaaat ttgatcagct attccaaaaa cggatcaaat ccggttttga aatgctagtc
    10201 aataattttt cagcaggtct tatcggtttt ggattagcgt tattaggttt ttctgcgatt
    10261 ggtccagttg tcgatgcatt gactcaagca atggcaaaag gtgtagagat cattttatct
    10321 gcccatttga ttccattgac aagtattttt attgaaccag caaaaatctt atttttgaat
    10381 aatgcaatca atcatgggat tttgacacca ttgggaactg aacaagtagc agaggctgga
    10441 aaatctattc tattcttgct tgaagcaaat cctggtccag gacttggcgt attacttgct
    10501 tttactttgt ttggcaaagg agcagcaaaa tcttcagcac caggagccat gatcattcac
    10561 tttttaggcg ggatccatga aatctatttt ccttacgtta tgatgaaacc gttattattc
    10621 ttatcagtta taggcggagg aatgtcagga agtttcattt tccaattgat ggatgctgga
    10681 ctaagagcgc cagcttctcc agggtcgatc attgcgattc tagcgatgac accgttcaat
    10741 tcttaccttc ctgttatttt gggcgtgatt gttgccgcag ctgtttcgtt tggtatttct
    10801 gcagtaattt tgaaagcgga tgcgaaagat acaaatgaag acttcgaaaa atctgttcaa
    10861 gagacaaagg cagctaagca agcttccaaa ggtgcccaag gtattcctca aggagctaca
    10921 ttctctggcg tacaaaaaat catttttgct tgtgatgcag gaatgggatc aagtgcgatg
    10981 ggcgcttcga tcttgagaaa aaaagtacag gaagcagact tgcctcaaac ggtgaccaat
    11041 caagcaatca gccaactgac agatgactcg aatacattga tcgttactca ggtcgaacta
    11101 caagaaagag caaaacaaaa agcaccgagc gcgcgttttg tctcagtaga aaactttttg
    11161 aattcacctc gatatgatga aatcattcaa gaattaagca gcaagcagaa aacatcagtt
    11221 tctgaaattg agaatgagcc agcagaagag tttctttcag caaacatcaa acaaatcaaa
    11281 gagattctac ttgtccacga tgatcgttcc ggatcagcaa caatggggat gaccgtgctc
    11341 aaagatatat tagacaagaa ccatttaact atcccgttaa gaaaagtcaa tatccaagat
    11401 ttaaaagatg aacctgattc actgatcgta gccaaagaaa gtttagcgga aaaagtacag
    11461 atggctgcac ctcaatccat tcatttatca gtcaacagca tggtcaatcc gcaaaaatac
    11521 gaatcgattg cacaatattt gaaacagaca gcttaaggga ggaaaacagc atgcaaagtt
    11581 tgccattcga catgatcgag ctcaatcgat cttttaaaac caaagaagaa gcgatccgct
    11641 attgcggtca gatgcttgta gaagcagggt gcgttgatga ggaatatatc gatgccatgg
    11701 tagatagaga caaaatgtta tctgtttata tggggaattt tatcgcgatt cctcatggaa
    11761 cagatgaagc aaaaaaatat gtgaaaaaat ccggcatctg tgtcgttcaa gtaccagatg
    11821 gtgtagattt tggtaccgaa gaagaagaaa agattgcgac tgttttgttc ggtattgcag
    11881 gagtcggaga tgagcatttg caacttgtac agcaaatcgc actttattgc agtgatatgg
    11941 acaatgttgt ccagctagca gacgcgctca gcaaagaaga agtaacggaa aatctagcaa
    12001 ttgcttaagg agagagaaaa atgagagcag tacattttgg tgcaggaaat atcggtcgag
    12061 gatttattgg agaggttttg gcagctaatg gatttgagat tactttcgta gatgtgaatc
    12121 acacgatcat tgatgcttta aatgaaagaa aagaatacga aatcgaatta gcagatgaat
    12181 caaaagaaaa aatcactgta aaaaatgtca aaggaatcaa caatcaaaca aatccagaaa
    12241 aagtaatcca agaaatcgct gaagcagatt tagttactac cgccattggc ccgaatatct
    12301 taccttttat tgctgagttg attgcaaaag gtatccaaga aagagaaaag gaaggaagca
    12361 caacatcttt agatatcatt gcctgtgaaa atatgattgg cggttcagca tttctagaaa
    12421 aagaagtgta taaatatctt ccggaaacat catttactga caaatatatc ggctttccaa
    12481 atgcagcagt cgatcgaatc gtcccattac aacaccatga agatccttta ttcgtccaag
    12541 tggaaccttt caaagaatgg gtcattgacg atagccaaag aaaaaataaa aaaatccaat
    12601 taaaaggggt tttatatgta gatgatctag aaccatatat cgaacgcaaa ctatttagtg
    12661 tcaatactgg tcatgcgact gtcgcttata caggtgcctt gttaggttac cagaccattg
    12721 atgaagcaat gcaagatgcg ttagttgtgg cacaattaaa atcagttttg caggaaacag
    12781 gaagtttgct gattgccaaa tgggggtttg atgcagagca gcatcatgcg tatattgaaa
    12841 aaatcattca tcgcttccaa aacaaaaata tctctgatgc gatcacacgt gtcgctcgga
    12901 caccattacg taagttaggt tatcaagaaa gatttacacg tccagtgaga gaactacaag
    12961 aacataactt gacttgccca catttgacgg caacaatggg gattattttt aattactatg
    13021 atcctgaaga tgaacaaagc cgtcaattgc atgaaatgaa gatccatgaa aacttggaac
    13081 aattgattca agaagtcaca ggaatcaatg atccgaaaac aatcgggaat atcaagcaaa
    13141 acgtgaaccg ttatgccaag caagtcgctt aaaataggat acgaaaaagg ggaatgtgac
    13201 gaaatttttg tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg
    13261 cagatccaat tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag
    13321 gttctcattt cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat
    13381 gacaccttca gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg
    13441 tcgttcacct tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc
    13501 attgaattct tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa
    13561 cgtgtagtga tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta
    13621 gtgacctagc aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata
    13681 gttgatcaaa gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc
    13741 ttgaccaccg ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact
    13801 aacgatctcc gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag
    13861 gtttccgtta tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc
    13921 ataattagcc acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa
    13981 gctgcaatac agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga
    14041 ttttttcgat tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg
    14101 caacttttag acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga
    14161 tatccatatc ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt
    14221 ctgcgtaatt ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca
    14281 tatcgattac ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc
    14341 cattgatatt gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt
    14401 tgattttatc aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt
    14461 agacattata cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg
    14521 ctgacacatt gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga
    14581 agtcacaatt tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca
    14641 agatgtgatg acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac
    14701 gatcttcttc atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga
    14761 agattggtga atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta
    14821 caatcatttt ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat
    14881 ttgtaaataa gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat
    14941 ctatttctag gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg
    15001 atacaattcc ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct
    15061 ggcgcaaaat attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa
    15121 cgacgacttg gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta
    15181 gtttacctgg taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt
    15241 ttccaactgc aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt
    15301 gctgacgaat aaatccaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc
    15361 ttattcttaa gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt
    15421 aacaaaaaaa cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc
    15481 tcttacctat attcccattt taaccgataa taattttctc gcttggatat tcatagccta
    15541 gatctcgtgt ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa
    15601 taaacatcag tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta
    15661 aagataaacc agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt
    15721 gttcttctgt agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa
    15781 agacaacgac tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt
    15841 tatcttcact tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc
    15901 gaatcccgcc gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga
    15961 tcagtgttgt gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg
    16021 taatcgaata aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat
    16081 ggtccttttc caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta
    16141 acacagctaa tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt
    16201 ataatagcca ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca
    16261 aaaacatgat caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag
    16321 tgacgtcaaa cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg
    16381 catcacgcca acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa
    16441 aaagtaattg aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag
    16501 acaaacgcgg ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc
    16561 gtttagaaaa aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa
    16621 ataatatttc cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg
    16681 atagtccagt cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat
    16741 ggctatctgg ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca
    16801 atatataata aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc
    16861 ctcgccgctg cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat
    16921 agtcgtttct accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat
    16981 aacaatacag ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa
    17041 gaaggaggag gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc
    17101 aaaaaataaa ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag
    17161 taaagcctaa gatattaata catacatgct gtgctccttg cagtacgcac acattggaat
    17221 tcatgtctca atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa
    17281 gtgagtattt gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa
    17341 ctgaccatca tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg
    17401 ttttaacaaa ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca
    17461 atatgcgatt agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa
    17521 gtgccctgac catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata
    17581 tccaaaagat ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg
    17641 aacgctcttt gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg
    17701 tgttcgctgc caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt
    17761 acttaaaaac atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta
    17821 agaaaaaaat aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa
    17881 agtaaggaaa caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct
    17941 attttgaagc caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt
    18001 gtgaatagtt ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca
    18061 ccaactactt cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact
    18121 acgacaagta catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact
    18181 gacagctcaa gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat
    18241 ccaagtacaa gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta
    18301 gatgataaca cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga
    18361 aatacccaaa cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta
    18421 tcaacggggt gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg
    18481 gatatacgat tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt
    18541 ggcaaaacca aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt
    18601 attttactgt tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca
    18661 ctcagctttt tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga
    18721 tagataatgg aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg
    18781 aagattatca attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca
    18841 aaacaaatga actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt
    18901 attactatgt caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact
    18961 cggacatgga aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg
    19021 tcggattgtt catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt
    19081 atcgtctgaa attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc
    19141 gtagccaaag aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata
    19201 aaaacgttta ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata
    19261 taacgagaaa gataagacga acacatcact tattctttcc tttcatttat aaacattgta
    19321 gaataaagaa agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca
    19381 agtaggaggt aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta
    19441 gcggctattt tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg
    19501 atggacccta cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa
    19561 cgaatggata ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg
    19621 atcatcggta tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc
    19681 aaaaagtatg atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta
    19741 caaggagaat atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt
    19801 acgagtgact ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc
    19861 ttgatttcaa aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa
    19921 gttgttttcc gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag
    19981 aagctgtgac atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg
    20041 agttgctcac tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg
    20101 aagcgttttt gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga
    20161 caaagcccgt tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt
    20221 tcggatgact aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag
    20281 ctaaaatttc ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa
    20341 gtgatagaag gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga
    20401 tcgttcggac atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag
    20461 tttctgaagc atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa
    20521 gcccagttcc gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag
    20581 tagttagttc ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt
    20641 tagcgatgga atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa
    20701 ttttaactta ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg
    20761 agaatctaga aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg
    20821 tgtccgagaa atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa
    20881 atatatcgag gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt
    20941 aggctttatc actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg
    21001 ggaataaaaa aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa
    21061 ggttacgcag caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac
    21121 tctatatatg atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt
    21181 tttccaaatt tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa
    21241 aagtgagcaa tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg
    21301 ctgaaaatca agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa
    21361 ctatttttta agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca
    21421 cataattatg aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga
    21481 gatggtttag gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt
    21541 gaagaaacag acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg
    21601 atcgatgaag cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac
    21661 gaattggata ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt
    21721 gaaggatcaa aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt
    21781 tggttctgcc acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat
    21841 gaaggcgatc aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat
    21901 tactttcacc catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa
    21961 ttgaagaccg ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata
    22021 caaagcgttt tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt
    22081 atcgtcgtta taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa
    22141 aactggatga tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa
    22201 aagaagtctt agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata
    22261 aaaatatcat catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg
    22321 gcgatctttt caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga
    22381 tggaagctaa cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag
    22441 acaacgtata ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt
    22501 caactgaatc gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag
    22561 ctgaagaagt agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt
    22621 caggtgctgg cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac
    22681 caacagggat cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag
    22741 caatgaaaat cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt
    22801 atgatgccaa tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt
    22861 ataatttccc gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag
    22921 atcagatttt agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa
    22981 catcaaaact ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg
    23041 gcttcttctt ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg
    23101 gaacgaaaac aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa
    23161 gaagaacagc ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag
    23221 tatttgctag gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg
    23281 atccctcgac cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta
    23341 ccgttagaag tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca
    23401 gcaagaaaaa attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg
    23461 aaagaaaatg ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga
    23521 ccagtaatgg atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat
    23581 gaatgggaac tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct
    23641 gagaacgatg gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa
    23701 tcaaatggaa agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata
    23761 tttcagcaag catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa
    23821 cgaatgatcc aagtttatgg ttgaagttgt ggatgaaaaa tttt
//
LOCUS       DAZAFF010000039        23829 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000039 DAZAFF010000000
VERSION     DAZAFF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.039"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACG9BB_001465"
     CDS             complement(53..787)
                     /locus_tag="ACG9BB_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFN2837410.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACG9BB_001466"
     CDS             1114..1524
                     /locus_tag="ACG9BB_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFN2837411.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACG9BB_001467"
     CDS             1517..2218
                     /locus_tag="ACG9BB_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFN2837412.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACG9BB_001468"
     CDS             2218..3804
                     /locus_tag="ACG9BB_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2837413.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACG9BB_001469"
     CDS             3823..4044
                     /locus_tag="ACG9BB_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFN2837414.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACG9BB_001470"
     CDS             4041..4802
                     /locus_tag="ACG9BB_001470"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFN2837415.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACG9BB_001471"
     CDS             5182..5691
                     /locus_tag="ACG9BB_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFN2837416.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACG9BB_001472"
     CDS             complement(5766..6305)
                     /locus_tag="ACG9BB_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFN2837417.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACG9BB_001473"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACG9BB_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFN2837418.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACG9BB_001474"
     CDS             7318..8169
                     /locus_tag="ACG9BB_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFN2837419.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACG9BB_001475"
     CDS             8197..8832
                     /locus_tag="ACG9BB_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFN2837420.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACG9BB_001476"
     CDS             8852..9625
                     /locus_tag="ACG9BB_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN2837421.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACG9BB_001477"
     CDS             10033..10830
                     /locus_tag="ACG9BB_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFN2837422.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACG9BB_001478"
     CDS             10808..11221
                     /locus_tag="ACG9BB_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFN2837423.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACG9BB_001479"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACG9BB_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFN2837424.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACG9BB_001480"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACG9BB_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFN2837425.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACG9BB_001481"
     CDS             15998..16558
                     /locus_tag="ACG9BB_001481"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFN2837426.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACG9BB_001482"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACG9BB_001482"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFN2837427.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACG9BB_001483"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACG9BB_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFN2837428.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACG9BB_001484"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACG9BB_001484"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFN2837429.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACG9BB_001485"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACG9BB_001485"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFN2837430.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACG9BB_001486"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACG9BB_001486"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFN2837431.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACG9BB_001487"
     CDS             21905..22483
                     /locus_tag="ACG9BB_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2837432.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACG9BB_001488"
     CDS             22535..23017
                     /locus_tag="ACG9BB_001488"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFN2837433.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACG9BB_001489"
     CDS             23134..23508
                     /locus_tag="ACG9BB_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837434.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23621..>23829)
                     /locus_tag="ACG9BB_001490"
     CDS             complement(23621..>23829)
                     /locus_tag="ACG9BB_001490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837435.1"
                     /translation="NLTTFENFRHKIFTTNDYYISIPTNNPNNTFSFLTPYPASSPAS
                     RTFFYQKNLYHPIDSTNQPNAVML"
BASE COUNT         7780 a         4286 c         5115 g         6648 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggtat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtttt
//
LOCUS       DAZAFF010000040        23680 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000040 DAZAFF010000000
VERSION     DAZAFF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.040"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..120)
                     /locus_tag="ACG9BB_001491"
     CDS             complement(1..120)
                     /locus_tag="ACG9BB_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFN2837436.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            299..1960
                     /locus_tag="ACG9BB_001492"
     CDS             299..1960
                     /locus_tag="ACG9BB_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN2837437.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2392)
                     /locus_tag="ACG9BB_001493"
     CDS             complement(2030..2392)
                     /locus_tag="ACG9BB_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFN2837438.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2460..3401
                     /gene="opp3b"
                     /locus_tag="ACG9BB_001494"
     CDS             2460..3401
                     /gene="opp3b"
                     /locus_tag="ACG9BB_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFN2837439.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3402..4445
                     /locus_tag="ACG9BB_001495"
     CDS             3402..4445
                     /locus_tag="ACG9BB_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2837440.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4451..5521
                     /locus_tag="ACG9BB_001496"
     CDS             4451..5521
                     /locus_tag="ACG9BB_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2837441.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5511..6464
                     /locus_tag="ACG9BB_001497"
     CDS             5511..6464
                     /locus_tag="ACG9BB_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2837442.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6476..7486
                     /locus_tag="ACG9BB_001498"
     CDS             6476..7486
                     /locus_tag="ACG9BB_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFN2837443.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7771..8592)
                     /locus_tag="ACG9BB_001499"
     CDS             complement(7771..8592)
                     /locus_tag="ACG9BB_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFN2837444.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8689..8967)
                     /locus_tag="ACG9BB_001500"
     CDS             complement(8689..8967)
                     /locus_tag="ACG9BB_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837445.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9235..11244
                     /gene="metG"
                     /locus_tag="ACG9BB_001501"
     CDS             9235..11244
                     /gene="metG"
                     /locus_tag="ACG9BB_001501"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFN2837446.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11723..12310)
                     /locus_tag="ACG9BB_001502"
     CDS             complement(11723..12310)
                     /locus_tag="ACG9BB_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837447.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12485..13807
                     /locus_tag="ACG9BB_001503"
     CDS             12485..13807
                     /locus_tag="ACG9BB_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN2837448.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13962..14738
                     /locus_tag="ACG9BB_001504"
     CDS             13962..14738
                     /locus_tag="ACG9BB_001504"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFN2837449.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14731..15300
                     /gene="rnmV"
                     /locus_tag="ACG9BB_001505"
     CDS             14731..15300
                     /gene="rnmV"
                     /locus_tag="ACG9BB_001505"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFN2837450.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15293..16177
                     /gene="rsmA"
                     /locus_tag="ACG9BB_001506"
     CDS             15293..16177
                     /gene="rsmA"
                     /locus_tag="ACG9BB_001506"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFN2837451.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16628..17257
                     /locus_tag="ACG9BB_001507"
     CDS             16628..17257
                     /locus_tag="ACG9BB_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837452.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17381..18490)
                     /locus_tag="ACG9BB_001508"
     CDS             complement(17381..18490)
                     /locus_tag="ACG9BB_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2837453.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18699..19121
                     /gene="mgsA"
                     /locus_tag="ACG9BB_001509"
     CDS             18699..19121
                     /gene="mgsA"
                     /locus_tag="ACG9BB_001509"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFN2837454.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19308..19406
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19444..19980
                     /locus_tag="ACG9BB_001510"
     CDS             19444..19980
                     /locus_tag="ACG9BB_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFN2837455.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKNLRSLAIHAK"
     gene            complement(20020..21783)
                     /locus_tag="ACG9BB_001511"
     CDS             complement(20020..21783)
                     /locus_tag="ACG9BB_001511"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2837456.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21786..23501)
                     /locus_tag="ACG9BB_001512"
     CDS             complement(21786..23501)
                     /locus_tag="ACG9BB_001512"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2837457.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7643 a         4323 c         4803 g         6911 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgtggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt tttcatttga ttgctccttt atttttggtt
     2401 cattatctaa taatgtagcg aaacatctgt cagaagtcaa aagctagggg gaaacattag
     2461 tgaatagtta tatcaaatat gtattaaaac gtgtcttttt catgatcatc acgctttggt
     2521 taattgcaac aatcaccttt ttcttgatgc aattgctgcc gggcacgcca tatacgaatc
     2581 aggaacgttt gagtcctgaa acaatcgaga tgctaaataa acaagtcggt ttggataaac
     2641 cagttatcgt acaatatggg atttatcttt ctaatttgct tcaaggagat tttggaattt
     2701 ccttccaatt taaaaaccag ccagtcgcac atttgttagc tggaagaatc ggaccatcct
     2761 tgcagttagg gttacaggca attatctttg gaacagtcct agggacgatt ttaggaacga
     2821 tctctgccat gaagcaaaat acatgggcag atacatcttc tacactcgta gcgatattag
     2881 gtcgttccat tccaaacttt gtatttgctg ttttattaca atatatcttt gcaatcaaat
     2941 tacaagtttt gccaatcgca aaatgggatg gctttgtgta cacaatctta ccaacaattg
     3001 cgttggccat gtcgccgctt gctgactctg ctcggtttat ccgtacggaa atggtggaag
     3061 tactgcatag tgattatgtg gaattagcaa aagcaaaagg attgagccgt tgggaaatcg
     3121 cctttaaaca tggtcttcga aatagtttga ttccattgat gactttatta ggaccgttag
     3181 ctgtagcatt gatgaccgga tcactggtgg tagaaaatat ctttgctatt cctggtatcg
     3241 gggaacaatt cgttaagtca atcacaacaa atgattatcc tacgatcatg gctgtaacga
     3301 tcctttactc atttatgctg atttttgtta ttttgatcgt ggatctattg tacggtctag
     3361 ttgatccaag aattcgtgtt tcagaaggga gccgaggata aatggaaatg ataccaaagc
     3421 gttatgaaac agttgcagat atccctgcgg atgaatttct tccattacaa aaaaataccg
     3481 aagaagaacg tgaacagatt actgcacctt ctttgaattt tatccaagat tcatggcgcc
     3541 gtttaaagaa aaacaaagct gccgttattt caatggtttt actattggtc atcattttga
     3601 tctctgtgat cacaatcttt gtttctcctc atgatccaac tgcccaaaac gtggcttata
     3661 tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg attgaatgga aaagcaatgg
     3721 ttggtggtga acttgttgat aaatatgctc aagcaaacgt cccttcaaac gtaaatttct
     3781 ttttaggaac agacggtttg ggacgtgatg tattaagccg cttatttatg ggaatgagaa
     3841 tctcgttgct gatcgcattt attgctgcat tactagatgt aacgattggt gtagcttacg
     3901 ggttggtttc tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt tttttggaaa
     3961 tcttatcagg aattccaaac ctcgttgtca tgattttgat gttggtcgtt tttgaaccag
     4021 gaattttttc aatcgtggct gctatggcca tcacaaactg gataccaatg gcacgtatcg
     4081 ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt tttagctgga ttgacattag
     4141 gtgaatcaag aatgaaaatt gcattcaaac atattttgcc gaatatctct agtgtcatta
     4201 ttattcaaat gatgtttagt attccatctg ctattttctt cgaagccttt ttaagcttta
     4261 tcggtttagg tcttacgcct ccgtcagctt cattaggaac gatgttgagc gaagggtaca
     4321 agacattcct gtatctgcct tatctattat ggattccggc agtaacttta tcggttatca
     4381 tgattggctt taacttactt gctgatggat tgcgtgatgc ctttgatcct aaaatgaaag
     4441 agtgaagacg atgacgaaaa ttttagaagt gaaagactta gaaatctctt ttgatacgta
     4501 tgctggaaag gtacgagcga ttcgcggtgt caattttcat ttgaataaag gagaaacatt
     4561 ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg accagaagca tcatgcgctt
     4621 gctgtcaagc aacgccaata tagatgctgg tcaaatcctc tttaaaggta acgatatcgt
     4681 tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga tgattttcca
     4741 agatccaatg acctctttag atccgacgat gcccattggg aagcaagtcg ctgagtccct
     4801 tagaaagcat aataaagttt caaaaaaaga gggacaaaaa gctgcattag aattgcttaa
     4861 attagttgga atacccgaag ctgagaaacg tatcaacagt tatccgcatc agttctcagg
     4921 aggtcagcgt caacggatcg tgatcgcgat tgcactggta tgttatccgg aaatcctgat
     4981 tgctgatgaa cctacaacag cgcttgatgt gactattcaa gcacagattt tggagctgct
     5041 gaaagaaatt cagacaaaaa tcgatacatc aattatcttt atcactcatg accttggtgt
     5101 agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg ttgaagttgg
     5161 aacatcggaa gaaattttct acaacccgca acatccctat acttggggac tgcttggttc
     5221 aatgccgaca ttagatagtg cgaacgatcg attgtatgct attcctggtt ctccaccgga
     5281 tttattgaac ccgccaaaag gagacgcatt ctatccaaga aatgaattcg cgatgaaaat
     5341 cgatgcagaa caagaaccac cattttttga actatcaaaa acacacaagg cagcaacttg
     5401 gctacttgct ccgcaggcac ccaaagtgac accgccagaa gaaatccaac gtcgctgggc
     5461 tatctttaaa gagaaacaaa aacatactta cggtcaagga ggaattgctg atgccaaata
     5521 aactacttga agttaaagga ttgaaacagt atttcaatgt aggaaaaaag aatgaagtac
     5581 atgctgtaaa tgacatcagc tttcacattt atgaaggaga aacattcggt ttagttggag
     5641 aatctggaag cggaaaatcc actactggcc gtacgatcat tcgcttgaat gaacctactg
     5701 atggtgagat attattcgac ggtcaagata taacgaagat caaagataaa aaaggtttga
     5761 cgaaattccg gcatgatgtt cagatgatct ttcaagatcc atacgcttcg ttgaatcctc
     5821 gtatgaaagt cagagatatc attgccgaag gaattgatgt taatggacta gctaaatcat
     5881 ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg aatccaagcc
     5941 acggtactcg ttatcctcat gaattttcag gtgggcaacg ccagcgtatt ggtattgcac
     6001 gagcattggc agtaaaaccg cgttttatca tttgtgatga accaatttct gcactagatg
     6061 tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa catcaattga
     6121 cctatctatt tattgcacat gatttgtcga tggttaaaca tattagtgac cgaatcggtg
     6181 taatgcataa cggcttgtta ttagagatgg gaacaagtga tgaaatctat aatcacggtg
     6241 ttcatcctta tacagaaagt ttgttatctg ctattcctct accagatcca gaccatgaga
     6301 gaaaacgcag aagaatcaaa tatcaacctg aaccagatga cggacaagta cgtcaacttc
     6361 gtgaaatcgc accagaacat tttgtctatg caactgagca ggaagtccca tattacgcaa
     6421 aaaaactaaa acgacaaaag gaatctcttc tggttgctaa ctaggaggga catagatgct
     6481 aaaagctttt aaattccgta tgtatccgac agaagaacaa aaacagcaat tgattcgtac
     6541 ttttggatgt gctcgattta cctataacca tttgctgaaa aagcggcaga aaagttggca
     6601 acaaacagga gtagccaatt tttcgctcac acccgctaca ttaaaaaaag aatacccatt
     6661 cttaaaagaa gtagacagtc ttgctttagc aaatgcacag cttaatttgg atcgggcttt
     6721 tcgcaattat ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa aaagtatgtg
     6781 gcaatcttat acgaccaaca accaaacccg tacgatctat ctggaaaatg gctacttaaa
     6841 gttaccaaaa caaaaagaac taatcaagat aaatcagcat cggccagttg aaggatcgat
     6901 tcgctccgct acgatttctg ctcgatataa tgaagaattt tatgtcgcac tactttgtga
     6961 tgtttcgcca gtaaaaaaag aatctttagc taaatggatc gggatcgcct atcatccgaa
     7021 gacattgatc caaacatctc gacctctaga agtaacttta ccaaaatttc accagacaga
     7081 agaaaagctg caacatgcac agcggaaatt gaatgtgaaa gtccgctccg ctcaccatag
     7141 aaagatcagg ttagatcagg caagcaatta tcagaaacaa aagagaaaag taatggatct
     7201 ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa caattatctg ggaaattggt
     7261 caaacaatat gactacttat ttgtagaatc ttttccaaaa gaagaggcac atgctgattt
     7321 ttctattcac gactggcata aattaataac aaaattgcgt tataagtccc aatggtataa
     7381 taaaaaattt cttttgatca ataccgacgg agcagaagaa agcaattccg taagaaagag
     7441 ccaagtagta gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa tgaatgaaac
     7501 tgataggagc gttagggtta gccttgttaa taaaaagtct taatgacttt tgtttctagg
     7561 aatttttctt ttgaagaaaa gctcactaat tatgacaaac caataaaaca atagcagaaa
     7621 aagttcgtac gagctttttc tgctattgtt aattttttat tcagaaagtt tacgaataca
     7681 tcaaaattct gaaacaaaaa acgtaactca caaagagttg tttttgcgtt ttttcttgaa
     7741 tgattaggtc tgaatctttt gctcacaacc ttattcatgg tgccccggct tgagctgata
     7801 gatttttgaa ggacggccga ttcctacagg acgttcaccg atctcatcga aatattggag
     7861 catagctttc ttaaaattgg aatgatcgat cgttttataa tcgatcccta agaatttagc
     7921 aaatactttt cgagcttccg taatcgtgaa atctttacct aaaacttgta gaacttgcgg
     7981 ctcgtgttcc attttattga ctacacggtt gaatgctttc aagatgattt gactatggtc
     8041 aaaagccaaa gtatctttgc cagaagactc tccagtgact aaatcaagcg agatagccac
     8101 ttctccgcta gataaatgga tcttgttact atgacgttct agtgtgaacc agcgtacttc
     8161 cttggcatca tctccggcga ttagcggttc ttctccaata aaggctaagt agctgactgt
     8221 gacgacccag cctcgcggat ctcgattagg cgtactgaat gtatgcagtt gctcaatatt
     8281 ttcttttgaa ataaccacac cagtttcttc ctttgtttct cttaagacgc tttctccagt
     8341 tgactcatcc ttttggacaa agccaccagg caatgcccat gaatttcgat aaggatgccc
     8401 ttttctttgg atcaacaaaa ctttcagctg atcttcttct ttgttatagc acatcagtac
     8461 gatatcgaca gtcaaagagg gcttgtcata ttctggacgt tcctgttttt gataccagtt
     8521 aagaaattct tcttcacttg ctttatgttc ataatataat ttttcttctt ctttagaagc
     8581 aaaacggttc atctgaccac ctccattgac agtaaaagta ctttttacct tattgtagca
     8641 gactctgaaa aaaaaccaag gatttacttg aaaaatctag gttatcattt aaatatgaaa
     8701 aataaatgga agtatgagat tgaataaaaa gaaaagcaga taaaacggca atactttata
     8761 ccgctcttca cgaatggcaa agaagccaca aataacgata tatacgtcaa tgataaaacg
     8821 caaaatccaa gctgctgtca taaaactgtt caaatgccca tggaggttaa aataaccata
     8881 catacgaata taaaaatagc tgaatattaa atgtgctaat gaaagaaaag caatactaag
     8941 tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc ccttactaag atagatagtc
     9001 tttattatac cacgtgtgag gggagaaata aaaagccgtt ttttagtaga tatgaaacaa
     9061 aacaaaaacc cgcatttatt caacctattc atccttttaa aggatagatt gaacgtagat
     9121 agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc taacagcatg ctttttttat
     9181 ggtatactag caaggaaacg aattcgcatc atgcgataaa ggaggaagag ccccgtggca
     9241 gaaaaggaaa ctttttatat cactacgcca atctattatc caagcggcaa acttcatatc
     9301 ggcaactcgt atactacgat tgcatgtgac gcaattgctc gttataaacg gttgatgggc
     9361 tttgatgtgt tttatctcac tggagtggat gaacatggtc aaaaaatcga aacaaaagca
     9421 gaagaactag gtgtaaaacc gcaagaatat gtagataaaa tggcggctga tgtcaagaaa
     9481 ttatggaaaa cgcttgatat cagttatgat aaattcattc gcacgacaga tgattaccat
     9541 aaagcagctg ttcaaaaaat atttgatcgt ttattagaac agggagatat ttacttaggt
     9601 gaatatgaag gctggtactc tgtttctgac gaagaattct ttacagaaac acaacttgca
     9661 gaagtgtaca aagatgacga aggtaacgtg atcggtggta aagcgccaag tggacacgaa
     9721 gtagaattgg taaaagaaga atcatacttc ttccgcatga gcaaatacgc agaccgtcta
     9781 ttggaatact atgaagaaca tcccgaattc atccagccag aatctcgtaa aaacgaaatg
     9841 atcaacaact ttatcaaacc aggattagag gatcttgcag tttcacgaac aacttttact
     9901 tggggagtac cagtcaagag tgatccgaaa catgtggtgt atgtatggat cgatgcatta
     9961 tccaattata ttactgcact aggatatggc tccgaagatg aaagtctatt cgaaaaatac
    10021 tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc gctttcatac gatctattgg
    10081 ccaatcatgc tgatggccct tgatttgcct ctacctaaaa aagtatttgg gcatggctgg
    10141 ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta tcctgaaatg
    10201 cttgtggaac gttatggttt agatgcactc cgttattatt tgcttcgtgc tgttccgttt
    10261 gggtcagatg gtgtctttac gccagaagat tttgtttccc gtgtcaatta tgatctggca
    10321 aatgatctag ggaacttatt gaatcgaacg attgctatga tcaacaaata ttgtgatgga
    10381 ctcgttcctg actatgcctc acttgtcact ccatttgata gtgaactgtc tacgacggct
    10441 gctaacgttg tcggacgtta ccatgatgca atggaaaaaa tggaattcaa tacagcattg
    10501 gctgaaatct gggtgctgat ttctcgtgca aacaaataca ttgatgaaac agaaccatgg
    10561 gtattagcaa aagacgaaga aaagaaagct gaattgaata gtgtcatgat ccatttagct
    10621 gaatctttgc gtatcgcagc gatattactt cagccaatca tgacagaaac gccagagaag
    10681 atcttcaatc aactaggatt ggactctgaa acaatgaatc ttgaaggatt acactttggc
    10741 gaatttcctt caggaacaaa agtagttgca aaaggtacac caatcttccc acgtttagat
    10801 atggaagaag aagttgcttt tattcaagaa aaaatgtctg aaggtactca aacaaacgaa
    10861 gatacagtca aatgggatcc agaagaaaca gaacttgttt caacgaaaga aaaacaaatc
    10921 aaatttgatg tatttgaaaa agtagaacta aaagtagcag aagtcatcaa ctgtcaaaaa
    10981 gtagaagggg cagataagtt gctgcaattc cgactagatg ctggagacag ccaagaccgt
    11041 caaatcttgt caggaatcgc tgaattttat cctgatccaa gcgaattgat cgggaaaaaa
    11101 gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg gacaaatcag tcaaggaatg
    11161 atcctttctg cagaagcacc agatggctct ttacaagtaa tcgaagcacc aaaatccatg
    11221 ccaaatggct cagaaatcgc atagaaattc aaagggggta tgacactagt tgtgtcatac
    11281 cccctttttc cgaaatttga gaagcaattt tcgtaaattc cttcttatta cttgaagcca
    11341 atcatataat acagaaacag agaactatcc aaaataaatc ctagaaaaag aagtacgagc
    11401 ttcgctgatt tttgttaatt tagaataata ctaataggaa tcgtaaaaac agaaaaaaag
    11461 cataatgata aagggtcggg cagattttaa ttgattcatc tggtactctt ttttattgtc
    11521 cttacattca taaagtttga atgcaatgac tttattacat ctatatttat atctaacagt
    11581 agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt gtgctcttat
    11641 atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg atgattgttt
    11701 tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt tttgtcttca
    11761 aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca taccagaaaa
    11821 tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt aacaagatca
    11881 accctaaaag caaggagcca gctataaacc aatgattatc ccaatgcatg acttcactaa
    11941 gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc atccgccagt
    12001 tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa ataccgatag
    12061 gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc acaggacctt
    12121 tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt gataacgttg
    12181 caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac tgctgttgtt
    12241 ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga taagaacgct
    12301 taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac gaaaaataaa
    12361 aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa aaagaatggt
    12421 aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa gaaagaggat
    12481 gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag ctaccgttat
    12541 gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg ctagtactca
    12601 gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga tctgtgctgg
    12661 attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg taaaatatat
    12721 tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa gcttgattta
    12781 ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga cgaacttatt
    12841 acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt ctgtgacagg
    12901 gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtaacgt tgattgttaa
    12961 attaatacca attgctgtga ttgtcatatg gggattattg accccaggac aaggaactgt
    13021 ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat tgagcagtgc
    13081 gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa tggcagggga
    13141 aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga gttttgtaac
    13201 agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg atcatcttgc
    13261 cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa tcggtggaaa
    13321 gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt atacactaac
    13381 tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca gtaaacagct
    13441 gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc agttagcagt
    13501 agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt tgatttttgt
    13561 gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa aaaaagcgcc
    13621 ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta ttgcgattct
    13681 cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag cattgatcgg
    13741 tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa aacgctcaga
    13801 agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct agatcttcgt
    13861 gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact tctgcaagta
    13921 gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt gattctcata
    13981 cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag cgagcacaag
    14041 aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt gaaaagtctt
    14101 tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat ccgacagagg
    14161 ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact ttaccaaagg
    14221 ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg aaagaagtcc
    14281 aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta ccaatcagta
    14341 ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa gatgttcgag
    14401 atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa cgatttcttg
    14461 acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca ttagacgtcc
    14521 aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat gccccttatc
    14581 tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt tatacagtag
    14641 aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa acatggacaa
    14701 atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca agagattata
    14761 gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc agatacgata
    14821 gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca tgcccaagag
    14881 actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat aagaaaaatc
    14941 attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca agctaaacca
    15001 aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt agaagcattg
    15061 aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac tagacaagac
    15121 cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga aaaactaggt
    15181 gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt gaagatgttt
    15241 cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca acatgagtga
    15301 atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac atggtttttc
    15361 atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa ggaaaatcgt
    15421 agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag gaatcggtgc
    15481 attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga tcgatgatcg
    15541 tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta ttgtacatca
    15601 agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg aggagcttcc
    15661 gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga tgcattttct
    15721 tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag tagctgatcg
    15781 tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg ttcaatatta
    15841 tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc agccaaatgt
    15901 agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag tgacagacga
    15961 aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa cgctttggaa
    16021 taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag caaaaagctt
    16081 ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac aagaattcgc
    16141 cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt gacagaagtt
    16201 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatgt
    16261 ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga
    16321 ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa acagcttctc
    16381 atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact attatgccga
    16441 acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg atcaaaaaat
    16501 tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat gtgtcttgac
    16561 agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat attaggagag
    16621 agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat tgattgggat
    16681 gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt cctttacttt
    16741 ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg cagtggctgt
    16801 tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg cattgcgagc
    16861 attgtcacat cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc atccgagtat
    16921 cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt tcaattttgt
    16981 gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt actttattgg
    17041 taatatgcct gaaacatatt attctcaagg atatttttat acagtattca tcttgatggg
    17101 gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta tagcaggaac
    17161 actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa atcggaaaga
    17221 gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca aaaaaaaaac
    17281 tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc taaaaagagc
    17341 tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt ttgatgactt
    17401 gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg tcgactattt
    17461 cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta gaaccagttt
    17521 ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct gatttcacta
    17581 cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct ggacgaatac
    17641 cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt tttccttcag
    17701 gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat gtcacattaa
    17761 agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg ggtgtatcat
    17821 aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata acgattcggt
    17881 cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt gtttcaagac
    17941 gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca tctaaatttg
    18001 ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt cccaatgcca
    18061 cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat tctgtcagac
    18121 ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca tatttacgta
    18181 atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat gcgtagtttt
    18241 ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact tttccgccga
    18301 tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa gtagttgact
    18361 ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt gatagattaa
    18421 aatcagttac agaataattt tctgcattgt catatttttt atatacgttt ttcagagcca
    18481 tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt taaagtataa
    18541 atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt ttttttgtca
    18601 atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat acactggagt
    18661 aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct ttgatcgctc
    18721 atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca atattggaaa
    18781 aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca accggcttgc
    18841 cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga gccatgattt
    18901 cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca cagccccatg
    18961 aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg ttagctacga
    19021 atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta gattttcgaa
    19081 gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc gtatggaaaa
    19141 ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga taatatgcga
    19201 tataatacga acgtattgga taatataaac aataatatta gtttttcgta tttgcagtaa
    19261 atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac agagtagaaa
    19321 ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga cgtaatgttc
    19381 gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa ggagatgcta
    19441 aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt attgattgca
    19501 ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa aatcagtgtc
    19561 acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc agggataggc
    19621 gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt ctttattgga
    19681 tttacattga atgcatttat tgaaggagca atctacggat tctttttcta tcgaaaagag
    19741 atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat caacttattt
    19801 ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg ggtcgtttgg
    19861 gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc catttattac
    19921 gtaggacgta gtattcctta caaaaaaaat ttaagaagtt tagcgatcca cgctaaataa
    19981 aaaacaagca gtcttcccat cccagaagac tgcttgtttt tattgtgctg tgaattgtgc
    20041 ttgatagagc cgtcggtaat aaccatcttt ttgaagcagc tcatcatggg tgcccatttc
    20101 gacaatagct ccctgatcca tcactaatat ctggtccgca tttttgattg tggacaggcg
    20161 atgggcaatc acaaaactgg tttttccttc catcattgct aagaaggctt gttggatttt
    20221 tttctctgtc aatgtatcga ctgagctggt agcttcatct aagatcaaca tagggggatt
    20281 actgatcatc gttcgagcaa tcgtcaacag ttggcgttgt ccatctgata tctttaaccc
    20341 ttgaccgcca atcttagtat ctaatttttc cggcaaacgt tctacaaatt catacatata
    20401 agaggctttt agggcatcat tgatttctgc gtcacttgct tccggattcc caaaacgaag
    20461 attttctcgc aatgtactgt caaatagcca agtatcttgt aacaccattc caaaactttt
    20521 acgtaatgta tcacgttgga tagagcggat gtcatggtca tcgactgtaa ttgttccttt
    20581 tgttgtgtca taaaaacgca tcagcaaatt aacaagtgtg gattttccgg cccctgtttt
    20641 tcctactata gcaatggttt gacctggata agcagtaaaa ttgaaatctt cgatcaaacg
    20701 ctgcttgggg tcataagaaa aagaaacatg atcaaaagag acttttcctt tgatattttt
    20761 gggttccaag gcataggcat ctggcaaatc aggcttttct tctggctgat cgatcaaatc
    20821 aaatgctcgt tctagaccag aaaaagcggt ttgcagttgt gtcgtgattc cagaaagttc
    20881 gataaatggt ttagaaaact gacttgagta gatcgtaaag ctggaaatca caccaacagt
    20941 gattgacgaa ccactcttca atgccaatag gccgccaact aaaccaatcg ataaataagc
    21001 aaggtgatct acaaaacgag aagacgggtt tgtcaaggaa gaagaaaatt gtgcccgttg
    21061 tcctttttca tatagatcag catttagcag ttcaaattgc tgttgactag cttcttcttg
    21121 ctgaaaagct ttcacgattt tctggttacc gattcgttcg gtcacaaagc ccgagatatc
    21181 cccaataatc ctttgctgtc tatcgaaatt tgtttgtgaa gcacgagcaa cgatccaact
    21241 cacaaggaaa ataattggtg tagaacaaag aacgaccaat gtcagaagtg gacttagacg
    21301 catcataaaa ataaacgaaa gaatgatgat tgccactcca gaaaataatt gattgaaggc
    21361 cgcagaaatt gcaatggaaa tattgtccat atcatttgta aatcgactga cgatatttcc
    21421 gtgggagttt tgatcataat aacgaagtgg caatcgattc agatgagcaa atgcatcttt
    21481 tcttaatttt gcaacggata agtaagctaa ccgattgctt aacacttgga tgagccattg
    21541 actgactacg ttgattaaaa cgataccacc gaatagaata agtaaatgga acagtcgatt
    21601 gaagcggacc tgtccttctc caatcatttg atcgatactt tgaccaattt gtaacgtcat
    21661 cacaactgtt gcaatcccat tgatgattcc tagtatcaca gctagccaaa tgtcttttgc
    21721 ataggcagta aggtaaggaa tgaatcggcg gagcgtctgc aaggataatt tttttacttt
    21781 cataactatt cttcctcctg tgatgcgacg atttcctgat attctgctga ttgagcaagt
    21841 aattcttcat gggtaccctg agcagcaatc ttaccttgat ccatcactaa gatatgatgt
    21901 gcttcttgaa tagagcggac acgttgggag ataataatca ctgtactgcc aagataagtc
    21961 tgtagcgcct tacgcaaatc caagtctgtt tgataatcca acgcacttaa agagtcatct
    22021 aaaatcagta atgctggctt ggcaatcaaa gctcttgcaa tcgtcagtcg ctgtttttgt
    22081 ccgccagaga aatttttccc attttccatg acttttgtat ctaaaccatt tggaagctga
    22141 cggacaaatt cttctgcctg agcgattttc agagcttccc aacattcttt ttctgtggcg
    22201 tctttcttac cccattgaag gttttcacga atagtcccgg taaatagtac agaagtttgg
    22261 ggaacaagag caatttgctg acggagatga tccagctgcc aagaacgaac atccattccg
    22321 tccattataa ctgtaccgga actgacatca tagaaacgcg gaatcaattg tgtcaaagtc
    22381 gttttcccgc ttccggttgg accaatgatt ccaaggactg tatttcgctt cagctgaaat
    22441 gtaatatttt ggagagaaag accagaatca ggagtataac ggaagtcaac atggtcaaaa
    22501 gcgataattg cttcagaaga aaaatcggga gtagtactga cctcagcttc aagaatcgaa
    22561 ttttctgtgt ctagcacctc tttcacccga tttcctgaag cttcggcacg tgtgaagatc
    22621 acgacgaggt ttgaaacaac gatcagtgcc aaaagcattt gattcatata attgatcaaa
    22681 gcaagaactt gtccttgttg caagctacca atatttactt tgatcccgcc aactgtcagc
    22741 aaacagatga tcccgacatt catgatcaag gttgtagcag gtgataatag ggcagagata
    22801 ttggaaacac gtaaataatt gttcgctaga tcatttgtgc ttttatcgaa cttctctgtt
    22861 tctttttttg tctgcgcaaa tgcgcggatg acacgaacac cgctaagatt ctgactaacg
    22921 tgttcattga gctgatctag tttttcttgc actttttggt agagaggaac ggtttttttg
    22981 atgataaaaa ataaaatgat tgagaaaata ggcaataatg ctaagaaaaa cagacctact
    23041 tcccagtcga tcacaaaagc catgaccact gacccgatac ttaaaaacgg tgctctgatc
    23101 aataaacgaa tgaccattgc taaagctagc tgaagctgat tgatgtcgtt tgtcattcgg
    23161 gtgatcaagg tatctgttcc aaaatgattc aactcgttgt gagagaaagt attgattttt
    23221 ttgattagct gattacgtaa ctctgtacca aatccttgtg aagctactga agcatagtat
    23281 tggcaaatga tagcacaaat cagaccaatc acagacatag cgaccatcca taagcccatt
    23341 tcaatgattt ttcctcggtc attcattttt agtccattat cgatcaagga agccatcatt
    23401 aaaggcagga caagttcgaa gacagcttca agaaatttga agaacgggcc caaaatgatt
    23461 tgcttacgat agtcttttgc ataacgtaat aactgaatca tatgattcct cctattattt
    23521 atttgtccac aattgaaaaa acaagtacta ccacattgta gcttaggaaa tttagaatga
    23581 aaagagaaca agccttttca taaatgaaag ttttattatg aagaagcttt agaaattgaa
    23641 tagactattt tttttaaaaa aaagaaaatg agaaaacatg
//
LOCUS       DAZAFF010000041        23455 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000041 DAZAFF010000000
VERSION     DAZAFF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.041"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(291..1787)
                     /gene="lysS"
                     /locus_tag="ACG9BB_001513"
     CDS             complement(291..1787)
                     /gene="lysS"
                     /locus_tag="ACG9BB_001513"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFN2837458.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1898..2902)
                     /gene="dusB"
                     /locus_tag="ACG9BB_001514"
     CDS             complement(1898..2902)
                     /gene="dusB"
                     /locus_tag="ACG9BB_001514"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFN2837459.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2903..3790)
                     /gene="hslO"
                     /locus_tag="ACG9BB_001515"
     CDS             complement(2903..3790)
                     /gene="hslO"
                     /locus_tag="ACG9BB_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFN2837460.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4007..6118)
                     /gene="ftsH"
                     /locus_tag="ACG9BB_001516"
     CDS             complement(4007..6118)
                     /gene="ftsH"
                     /locus_tag="ACG9BB_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFN2837461.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6208..6753)
                     /gene="hpt"
                     /locus_tag="ACG9BB_001517"
     CDS             complement(6208..6753)
                     /gene="hpt"
                     /locus_tag="ACG9BB_001517"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFN2837462.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6753..8198)
                     /gene="tilS"
                     /locus_tag="ACG9BB_001518"
     CDS             complement(6753..8198)
                     /gene="tilS"
                     /locus_tag="ACG9BB_001518"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFN2837463.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8318..8788)
                     /locus_tag="ACG9BB_001519"
     CDS             complement(8318..8788)
                     /locus_tag="ACG9BB_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFN2837464.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8834..9259)
                     /locus_tag="ACG9BB_001520"
     CDS             complement(8834..9259)
                     /locus_tag="ACG9BB_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFN2837465.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9326..9595)
                     /locus_tag="ACG9BB_001521"
     CDS             complement(9326..9595)
                     /locus_tag="ACG9BB_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFN2837466.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9606..11201)
                     /locus_tag="ACG9BB_001522"
     CDS             complement(9606..11201)
                     /locus_tag="ACG9BB_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN2837467.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11216..14737)
                     /gene="mfd"
                     /locus_tag="ACG9BB_001523"
     CDS             complement(11216..14737)
                     /gene="mfd"
                     /locus_tag="ACG9BB_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFN2837468.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14754..15314)
                     /gene="pth"
                     /locus_tag="ACG9BB_001524"
     CDS             complement(14754..15314)
                     /gene="pth"
                     /locus_tag="ACG9BB_001524"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFN2837469.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15667..16641
                     /locus_tag="ACG9BB_001525"
     CDS             15667..16641
                     /locus_tag="ACG9BB_001525"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFN2837470.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16811..18211
                     /locus_tag="ACG9BB_001526"
     CDS             16811..18211
                     /locus_tag="ACG9BB_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFN2837471.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18289..19011)
                     /locus_tag="ACG9BB_001527"
     CDS             complement(18289..19011)
                     /locus_tag="ACG9BB_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2837472.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19090..19596)
                     /locus_tag="ACG9BB_001528"
     CDS             complement(19090..19596)
                     /locus_tag="ACG9BB_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN2837473.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19719..21518
                     /locus_tag="ACG9BB_001529"
     CDS             19719..21518
                     /locus_tag="ACG9BB_001529"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFN2837474.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            22012..23400
                     /gene="ltrA"
                     /locus_tag="ACG9BB_001530"
     CDS             22012..23400
                     /gene="ltrA"
                     /locus_tag="ACG9BB_001530"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFN2837475.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT         6654 a         5059 c         4325 g         7417 t
ORIGIN      
        1 ttagtaacat gtcaataact tttttgttat gtttttgaat aaatattttc ttctgtcttt
       61 tggacaagta ataatatacc aactaaattt agcttggtca acgaattttt tgtatttttt
      121 caagtttttt tcaataaacg attattcata acttttcttt ctttatcgtt cgttttcgtc
      181 ttctttaatg tctagaatag tcggcattag cttttgaact ttcgttatta tttattcctt
      241 tctacacagc aaaagctgcc taaggaattc cttagacagc ttttattcga ttatctcatt
      301 gttgggaata gcaaaacatc ccgaattgat tgtgcatcag ttagcaacat tactaatctg
      361 tcgatcccga tgcctagacc accagttggc ggcataccgt attctaaggc ttccaagaag
      421 tcttcgtcta caccgtgagc ttcgtcattt cctagttcac gttctttttc ttgcgcttcg
      481 aatctttctc tttgatcaat tggatcgttc agttctgtaa aggcattagc aaattcgcgc
      541 cccactatga atagttcgaa tcgatctgtg aatcgaggat cttctggatt tttcttcgct
      601 aaaggcgaaa cttcgactgg atgtccgtat acaaacgtcg gttgtgtcaa tgtttcttca
      661 acaaatgttt caaagaattc attgataaca tgtccgacac ccatagcttc ggttacttct
      721 acattgtgtt tttcggctaa agctaatgct tcttccaatg tcatttcttg ccagaagtct
      781 acaccagttt gttctttgat tgcatctacc atatggatgc gtttaaagtc actttctaag
      841 tcgacagctt gtccatcata tgtgattttt gctgttccta gtactttttc agctgcgtta
      901 cggataatac cttctgtcag attcattaca tcattgaaat cagtatatgc tgtgtatgct
      961 tctaacatcg taaattctgg attatgtgta gtatcaattc cttcattacg gaaaacgcgg
     1021 ccgatttcat aaactttttc cattccgcca acaattagtc gttttaaatg aagttctagc
     1081 gcaatacgca aatataaatc aatgtccagt gcattatgat gcgtgataaa tgggcgtgcg
     1141 gcagctccac ctgcctcatt gtgcaatact ggtgtttcta cttcaatata gccattgcca
     1201 tctaaatagc gacggatttc gctgataatt tggcttcgtt tcataaatcg gtcaaagctt
     1261 tcgcgattac tgattaaatc caagtagcgt tgtctgtagc gttgttcaat attcgttaag
     1321 ccatggtatt tatctggcag tggacgaagt gcttttgaca aaatcgtgat ttctgaagct
     1381 ttgatagaaa cttcgcctgt gtctgttttc atgatttgtc cagttacacc aaagaaatct
     1441 ccaaggtctg catgtttgaa tacttcatag gcctcatcac ctacttggtc ttttcttaca
     1501 taaatttgaa tttggccttc acgatcttgt agatgcgcaa atccagcttt cccttttcct
     1561 cggcgagtca tcatacgacc agctacacta gcggtcaatc ccatttctgc tagttcttct
     1621 tttgttcttg gatcaaatag ttcatgcaat tcttctgagt tgtgcgtgcg ttcaaatcgt
     1681 tttccaaatg gatcgattcc atcttcacgc aattgttcca ttttttgacg acgtacaagc
     1741 atttgatcat ttaaatcttc tacttgatgt tgttgttcct gtgtcacaaa agttcctcca
     1801 ttctcatgtt aatctctttc ctataatgcc aatgaaacac aaaaaaagca agccgaactt
     1861 ggaatttcgg cttgaaaatt cacgaaaaac ttagaaatca caacgtttgt ttcaatagta
     1921 ctttttccgc tttttctaca aattgatcca gcaggtcatt gatttcttgc tgtttttctg
     1981 tctgattgat tgctactttc actttcgctg ctcgaggaat cccttttaaa taataagccg
     2041 catgtgttct gaattctcta caagctactt tttctccttt gagatctgct aagcgttgta
     2101 aatgaagttt agctgtagca attttttcgg ctggtgttgg ttcaggaatc agttcccctg
     2161 tttcgagata atgctgtgtg cggtgaatca tccatggatt gcccagcgct gcacgaccaa
     2221 tcatcacacc gtctgcaccg acatagtcaa gcatccattt tgcatcttct ggcgttttga
     2281 catccccgtt ccccataaat ggaatcgtca aatgtttttt cacttctttc aatacttccc
     2341 agtccgcttt tccttcatac atctgaaccc gtgtacgtcc atgcatagcg atggcacttg
     2401 ctccagctga ttgtgctgct aatgcatttt ctacagcaaa gacgtgctct tcatcccaac
     2461 caatacgcat cttgattgtt actgggatat ttactgctga agaaacagct tggaccattt
     2521 cgtatacttt ctcaggatct aaaagccatt ttgctcctgc ttctgctttg attactttat
     2581 ttactggaca gcccatattg atatctatta tatcggcagc tgtgttttct tcgacaaatt
     2641 gagcagcttc aactagacta tctttatttc cgccaaatat ctgtaaactt aatggatgtt
     2701 ctgtttcttc aatgtggagc atctctaaag tttttttgtt tctgaaatga ataccttggt
     2761 cactgatcat ttcacatacg actaagcccg caccaaattc tttgacagtg actcgaaatg
     2821 ctgaatttgt tattccagcc atcggtgcaa caacgacacg attagggatt tccacattgc
     2881 caattttcca tgtgtgttcc acttaaatcc ctcctaaggc ttctttcttc aatccatcta
     2941 attcttcttc gctatacttg tatttgttat tacagaaaga acaaaccgct tctgcaccgt
     3001 gatcttcgtc tatcattgct tggatttctt ctgcacctaa cgcgataatt gcttctgcaa
     3061 atttttcttt tgaacagtca catttgaatt gaacaggcat tttttccaaa atgttcaatt
     3121 cttctgtacc aagcaatcct ttcaatattt cttctggtgt ctgtccttca tctaagaggg
     3181 tagagattct tggtgtcgtt tttaattgtt cttcgatctt gctgattgtt tcttcatcgg
     3241 cacctggcat gatttggatc atgaatcctc ccgccgtctt gatgctgtcg tcagtattga
     3301 ccaatacact cagtccaaca gctgaaggga tttgttctga gatagcaaga tagtaagtaa
     3361 aatcttcagc gatttctcca gaaacaatcg gcgtttgccc agaaaaaggt tcttttaatc
     3421 ctaagtcttt tgtgacagta aacattcctt gtgttccaac tgctccccgc acatcgattt
     3481 taccttcact atttagagga agactgagat gcggattttt gatgtaacct ttcacatctc
     3541 cctttccatt tgcatctact acaattcctg ttgctggacc atcgccttgg attcgaacag
     3601 ttagtttgtc ttctcctttg agcgtcgcac caagtagcag actaccgacc aatgtccgtc
     3661 ccaaagcagc tgaggaggtg tgccaagtat catgacgtct ttgtgcctcc gctacggtat
     3721 ttgttgcttg aacggcgtat gctcggacga atccgtcgtg agcaagtgcc ttaaccaaat
     3781 aatctttcat ttgttatgta ctcctttttc cgtttttaaa ctcatagtga atcatactag
     3841 caactactgc ttttttcaac tttggaatgc ttcttttaaa aataattcag aatcatctag
     3901 aaaacaggac atcaccctat ttacaactta aataaagaaa tctcccgaaa accggttttt
     3961 gttccggttt tcgggagtca tatcagatca attgacctat atttttttat ttgtattgat
     4021 cgtctgaatg attatcttct gattgttgag aatctgaatc atgttctgct ttattttcct
     4081 cattcagttc atgttttgct tcttcaaaat cacgtttttc ttccgcttgt ttttcagcgt
     4141 ctttttcttc caaagcacgt tttgcttctt caaaagtttt cgcttctttt tcgcttggat
     4201 aatcatttga ctcagtacct tcaggcatca caccgtgttc aaacaatgac ttgattgctt
     4261 tggcatccaa tgtttcgaat tccaaaagtt tttcagcaat cagtttatgc tgtgcacgat
     4321 gagcttcgat gatttctcga gctttatcat gagcttccat caagattttt cgtacttctt
     4381 gatcaatctc gaaagcgact tgttccgagt aagcttttgt ttgaccatag tcgcggccaa
     4441 caaacacttg atggtttcct tcatattgaa caggacctaa acgatcactc attccatatt
     4501 ctgttaccat gcttcgtgct agagctgtcg cttgctcaaa gtcattggaa gcaccagttg
     4561 attggacatt gaagatgatt tcttcagctg tacgtccacc aagtaagcct acgatttgtt
     4621 caaacatatc ttctttcgtc atcaagaact gatcttcttt tggtaaagcg atcatatatc
     4681 cgccggcgcg tccgcgaggg atgatcgtta ctttgtggac gacccgagcg cgactcaata
     4741 ctaatccgac aatcgtatga ccagcttcgt gataagcaac catttcgcgt tcttttttgc
     4801 tgattacgcg atctttttta gctggacctg caatcacgcg atcttccgct tcatcgatat
     4861 cagaagcatc gattttcttt ttatttcttc tagcagctac taacgctgct tcattcaaga
     4921 cattttctaa atctgctccg gcaaaacctg gtgtctgttg agccacaact ttcaagtcaa
     4981 cgtcatctgc catcggttta ttccgtgcat ggactcgcaa gattgcttca cgacctttta
     5041 catctggacg tccaactaaa atctggcggt caaaacggcc tggacgtagt aacgctgggt
     5101 ctagtacatc agaacggttg gttgcagcaa tgacgattac tccttcattt ccgtcgaagc
     5161 catccatttc tacaagcaat tggttcaatg tttgttcgcg ttcatcatgt ccgccgccca
     5221 taccggcacc acgttgacga cctaccgcat caatttcatc aatgaaaatg atagctggtg
     5281 cgtttttctt cgctgtttca aacaaatcac gaacacgact tgctccaaca ccgacaaaca
     5341 tttctacgaa atcagaaccc gagatcgagt aaaacggtac tccagcttct ccggcaacag
     5401 cttttgctaa taaggtttta cctgttcctg gaggtccttc taaaagtaca cctgccggga
     5461 tacgggcacc taattcaaca aatcggcgag gatctttcag aaattcaaca acttcaacta
     5521 gttcttgttt ttcttcttcc gcacccgcta catcagaaaa acgtacgcga ttagcttttt
     5581 tgtctgcttc tttggctttt gatttaccaa agttcatgac acggccgcca ccgccaccgc
     5641 cgccgccttg ttgtcccatc atcatgtaaa agaaaaagat gatgattacg attggcaaga
     5701 agctaagcaa tagggaaacc catataccgc tgcttgattc ttcttttact gttgtagcaa
     5761 ctttgttttc ttttgccagt tctgttactt ggctcaatgt tgtatcattt ggcaaaatga
     5821 ttgttgtgaa atgggtagta gtcgtatctg ttgaacctaa gatagctaac ccaccgctgt
     5881 tagcgacggt ctgctgttct ttgtattcac cagtgatctt atatacacca tttgcaggct
     5941 gcacttcaaa ttctttgatc ttgcctgctt gtagttgttc tgtgaatttt gagtactcga
     6001 tatctgacga tcgctggccg ttattaccaa aaatgaaata aacgaccatt accatcgcta
     6061 agattacgag gacataatac aaggcatttt tcatcatgcc gttgttcttt ttgttcatag
     6121 ttgccctccc taagcgtcaa tttaatttaa cagtttttta tcaatcctga ccattctatt
     6181 gtaccataat ttaattgaga tacagcatta atttgattga taaacactag gtttcaatac
     6241 accgatgtac ggaaggttac gatatgcttc tgcataatcc aagccataac cgaccacaaa
     6301 ttcatttggt acgttgaagc caacataatc tgcttcgata tttactactc ttccttcagg
     6361 tttgtcaagt agtgtaacaa ttttgactga ttttgctttg cggtatttga aaagatccac
     6421 taaataagca agtgttcttc cgctgtcaat gatatcttca acgatcaaca gatcgcgacc
     6481 ttctacattc gtatctaagt ctttgacgat ttttacttcc cctgacgaga ctgttgcatt
     6541 cccgtagctt gaaacatcca tgaaatccaa ttccaaatac gtatcgatag aacgaacgat
     6601 atctgccatg aatggtaccg ctccttttaa gacacctact actaggggat tggtgttttg
     6661 gtaaatttct gttaactctt tgcctagttc tttgcaacga acttgtatct cttcttgtga
     6721 gatcaaaatt ctctcaatat ccttttccaa catcaggttc tccttccaat tgcttctttt
     6781 tgatatttgt aaagaagtat gtagtgtatt ttatcagttt cttcacgaat actcaaatat
     6841 gaaaaggcaa aaggtatcaa gctatatata tttcttttgg catctgtaat gatccaagca
     6901 tgctgccttt gtgaattagg tactttctga tcgataaaat atctagatac tttttttgtg
     6961 agattctctg ttaatttaat gcggtctcct gcttttcttt tttcgacata cagttgcaca
     7021 tcagcaggaa tccaaatgtc atgagaaaat agagaccatg aatctgttgc tttgatgtcc
     7081 cctgcatttt caggtttgac cagaccaatc cattctgttt cattcaaata tattccttgt
     7141 tctggaatca gcaacaagcg ctggtcttct gtctggctag ttgcttgttt ttctttttta
     7201 gagagtacaa caaaatgata ctctcttttc agtatccaat tattgccaat actgagttgc
     7261 cattggcttt ttttctgttg ccactggtct aaaatcgact ccatttgttg ttctttcaaa
     7321 ataattcctg tttttgagaa tgctttgtcg aaaaaggagt aaaaaaaata gtatcgttca
     7381 gcatctgtca tttcttcaat catttctgca gaaaatcgaa attgttcctc ccactgttcg
     7441 acttcctttt cgatcaactg tcccatatat ttttgaatga cttggtctgc ccattggatc
     7501 tgttgcgaaa attgctggaa atgtctcaac gcctgtgtat tttcttgttt caattgagga
     7561 actaccaaat gacgaagccg atttctctgg atatctaaca attggtttgt ctgatcctca
     7621 aagtaaacaa gctgcgattc cgctgcataa gtatataggt cttctttgct aaaactcagc
     7681 aacggacgaa tcagtctgcc cgtagcaaat ggttgtactt ctttgattcc agaaaaagta
     7741 tttaactgtc ccccacgaac gattttcatt aacaccgtct cgatttgatc atctccgtga
     7801 tgagcagtca tcaacgtgtc atattgtcct tcttccatca cgcgagtgaa ttcttgataa
     7861 cgaaagttac gagcttctgt ttctatggcg gatttggctg gatcttccca aatacttaca
     7921 tataaaggga gattcttctc ttcacaatat gagcgaagat aggcagcttc ttctgctgat
     7981 tcttctctta attggtgatt gatgtggacg atacctaaag aaaaaccgat ttggcgttgc
     8041 acttgtaaaa gtaaatggag caaaaccatc gaatcaacac cacctgatac cgctgctaaa
     8101 attctcgatc cttgttgcca gaatccttgt tcattcccgt gccgaataaa tgactgctgt
     8161 aatagcggtg cttttttccc gcctttttca acgttcatcc tttccacacc ctttcttcct
     8221 aaaaaaaaga gagcgggaaa acgtctaaaa cgacaattaa cctgccctct gttgatacta
     8281 tgacccaaat agtcgcacta tcattatttg cggcttttta gctgcgacgt ccgccacggc
     8341 caccgcgctt tccttctgtg tttcgcttca atgaagataa gcggtcatcg ctatctttca
     8401 aaaatgaact catcaatgaa tcgaagtctt ctttttgagc tgaaggttga ctgcgagaaa
     8461 aagatttttt ctgtgctgga cgatttcggt tgtcacgggg atctttttgt tgatattttt
     8521 taggacggct ttcttctcca gattcagcag gttgttcctg cgctttccgg atagatagtc
     8581 cgatttttcc atcatcattt actgaaataa ctttaactgt tacttcatct cctacactta
     8641 aaacatcatg gatgtctttt acatagccat ttgacacttc gctaatatga actagacccg
     8701 tttttccagc acctagatca ataaacgcac caaaatttgt gattcctgat acttttcctt
     8761 gcagttttgc tcctacctcg attgacataa aaaaaatgtt cctcctaatt atttcccttt
     8821 tttatattcc tgcttatttt gcagttgtag aactagttga ttgatctgtt tcatttgtcg
     8881 agctgtctgt atttgattct aatataggat aaactttttc accgtctttt gagtaaaaga
     8941 atttgcttct ggcaagttta gctacatact catcatcttt cagcaatgaa acatcttggt
     9001 gcagctgatc aacgtctgcg ttgacttctt tcatttcagt atttgcttcg actctcaatt
     9061 cgttcaattg atgcatcctt tgcatgtcat ggaaaatctg cacacctatt atcgcgaaaa
     9121 tgaccaaagc accaacaaaa attgcagcca gtctgcgtct tcgaaaaatc agttgacgtt
     9181 gttgttgctg aaattcagca tactttttct ttgcatattc cgtatctagc gcagcgattt
     9241 tattgctttt tttcgtcact tggattcgct ccaatctttt tgattgtctt tttcattata
     9301 ccaatatcgt gaaagcttgt ctaatctatt tctctagtgt ttctgtgcga ttttcagaaa
     9361 taatttcgta cattttttgt gcatcttctt ttttggttga ttcatgtaat tccgtaattt
     9421 tcacttccaa cgttttgttg ccaaactgga ctttcaaaat atcgcctact ttgacatcac
     9481 tggaagattt cgccaattta ccgttgattt gaattcttcc tttatcagct acttcttttg
     9541 ctactgatcg tcttttgatg attcgggaaa tttttaaaaa tttatctaat cgcatatctt
     9601 attcctcact ttttaatttt tttatttaat cgcaggattt tcttaccaaa aggcaacatt
     9661 agccattctc gtactgtaaa cagacgaaga cgaatcactg cataaatgaa cacagaagcc
     9721 ccgatcccaa ctcctatcac gctgacaaaa aaagcagtgg aacgatgagt tacaggacca
     9781 aatactatat tcaatacacc ataatacagc agcagtacta ctgtcataca aagaaggctg
     9841 aacatcaact tccgtccaaa atgccgttct ttccaaaaag catttagctc ttttgtttca
     9901 attcgaacaa aataccaaag cgtcacacct aatcctaaaa gggtagacag gcttgcacct
     9961 gctgttccta ataaaccggt cagaaaagaa gtagtcaatc cttttaccaa tagtccccat
    10021 cctgcgcctt ttaaagaagg gcgaaacgag ttcttgcttt gagcaatact ttgataagct
    10081 tggatgactg cagtgaaagc aatggaaaaa acaaataata caagcgctgc atttcccgca
    10141 taatctttaa acaacgcata attgatataa gggagcagca aggctaatcc caaagacgca
    10201 gctaatgcta aagcggtcgt caatctaaga tagattttgg ctgtctgtaa aaactgacga
    10261 tagatccggt tggtcaaatg acgagtcaac gcaggcagga atgtcgcact taatgcagtg
    10321 gcaatcacta aacctaattg tactaaaggc tgacctcgat catagacccc ttttgctatc
    10381 ttcgcaccgt attctgacag tccgtaaact tccaaagcat tttttacaaa gaaagagtcg
    10441 atcagctgga aaaagataag caatccacta taaatcgaaa ccagtccgcc ttctaccaga
    10501 aaacgccgga tcaagggctt tgacggctgt ttgaacaatg gaaaccgtcg gaagcttaac
    10561 gctcctccat gaatcttttg ttcatagtag tgaaggatcc catatgcgca taaaccgcca
    10621 aaaacagctc cactcattgc tgccgtccct gtttggtaca ccgtccatcc aagctttcta
    10681 aaagcaaacg cactacagac aatcacaaaa acacgaacga actgctcaac tacttgagaa
    10741 acagcagttg gttccatcaa gaatcttcct tggaaattcc ctcgataaaa agatagacca
    10801 ggcatcagca aaaaggtaaa ggaaactact tggatcagcg gtttcaattg tatgtctccc
    10861 atcatccaag caatccagct gctaaatgca aagacaaaca accacaaaaa gacacctgtc
    10921 caaaatacta agggataaag attttgcaaa gcttgctttc gctcgaaagg attctttctt
    10981 tctgcaacgt actttgatat gaactgcgga agaccggata aagctaaagt cattgctatc
    11041 ccataaattg gataaacttg ctgataaaca taaaaacctt cgtcgccgac taagttttgg
    11101 aggggaattc ggtaaagggc acttaatact tttgctatga aagaagcaat cgtcaataca
    11161 aatgctcctt gcatgacgcg ccgcatttct ttatgatcca aatcattgct ccttcctacg
    11221 ctgacagata tttttgctca cgcaacgctt tgacaaactt ttggatttcc tgaagccata
    11281 ctgcttcttc catttggttg gggatcgtca agcgtatcac catctgttct ttttcaacgc
    11341 ctagactggc ttttagtttt gtcgctgtca gtgcttcgaa taactgttcc acactatagc
    11401 gttttgttcc aactttactt agagtaaaga ctatatgcgc ctcctgtttc cttatcgttt
    11461 ctaggagcgc tcgatcgcca tccattttga tctgcccaat tgtcaagaga tgtgctactt
    11521 cttctggata ttctccaaaa cggtcgagca gatcatcttc tagttcatct agcatttctt
    11581 gcgaatcaag ttcacgaatt cgtttataga tctcgatttt ctgccgttga tctgccacgt
    11641 atgtttcagg tagatacgca tcgacaccga ggtcgatctc aacagtggtc ttctccactt
    11701 gactattctt tccttgttta cgagaaacag cttcgcttag catttgagaa tacatatcga
    11761 aaccgactgc atcaataaat ccatgctgct gagcacctaa gagattacct gcaccgcgaa
    11821 tggaaagatc ccgcatcgca atcttgaatc cagatcccag ttctgtaaaa tctttgattg
    11881 cctgaagacg tttttcactg acttcgttaa ggatcttctg ctgctcatac ataaagtaag
    11941 catatgcgac acggctgctt cttcctaccc gccctcgcaa ttggtataac gtggataatc
    12001 ccatataatc agcgttctca acgaacaacg tatttgcgtt cgggatatct actcctgttt
    12061 caatgatggt tgtagtgaca agaacatcat attgaccttc aataaattca aaaagcgtat
    12121 tttctaattg cgcctctgtc atttgcccat gagcataacc gatccgtgct tccggaacga
    12181 gttcttggat ttcttctact ttttgttcga tcgtttctac ccgattatat aaatagaata
    12241 cttgtccacc tcgtcccatt tctcgatgga ttgcttcgcg aatagcccca ggattttttt
    12301 ccataacata cgtttggatc ggataacgat tcgcaggcgg tgtttcgatt actgaaagat
    12361 cccgcacgcc taacatcgac atatgcaaag ttctcggaat cggtgttgca gtcagagtca
    12421 aaacatccac ttgtgcacgt aactgtttca atcgttcctt atgttttaca ccaaaacgtt
    12481 gttcttcgtc aatgatcagt agacccaaat cgctaaattc aatatctttt gacaaaattc
    12541 ggtgggttcc tacaacaata tcaacttgac ctgtacgcag ttgttcgatg gtttcttttt
    12601 gttgctttct tgttctgaaa cgacttaata gcccgatatt tacggggaaa ccttcaaatc
    12661 tttccagcat cgtttcatag tgctgctgtg ccaagatcgt tgttggaact aaaaaggcta
    12721 cttgtttgct ttcctttatc gctttaaagg ccgctcgaag cgctacttct gttttcccat
    12781 aaccaacatc acctacgagt agtcgatcca ttggtttttc tttttccata tcccgtttga
    12841 tctccgctgc acttctcaac tggtcatcag tttcagagta ggggaaggca ttttcgaatt
    12901 ctttttggta ggcgtcatca ggtccaaaag catatccttt ttctgactct cttgcggcat
    12961 ataatttgat tagatcgtca gcgatatcct cgatttttga agagactttt cgcttggttt
    13021 tcgtccattc acttccgcct agcttattga tgcgaggcgc tttggcttca gaggcgacat
    13081 atttttggat cagattcagt tgagtaacag gaataaacag tttgtcgtcg ttttgataca
    13141 aaatagtcat atagtcttgg tgaacgccat ctacttctag cgtttccatc ccaatgtatt
    13201 tcccgatacc atgattggca tggacaacgt aatctcctgc ttttaactca ttatagcttt
    13261 tcaatcgctc tgcgtttgtt actgtttgac gtctcgtttg ttttttcgtc gttttttgga
    13321 aaatttcttt ttctgtgata gctactattt tttcttgagg aagttcaaaa ccagcctgca
    13381 aagctccttc aactaattgt acctgtcctc tgatcaatgc atttttttct gtttctacta
    13441 ctaatatgtc ttcatctcga agcatttgtt ctgctttgcg tattctgtcc tttgtcggaa
    13501 taaagatcac aacagtctgt ttttgcttgc gccaacggtc catttctgtt ttcagcaatg
    13561 gcatttgtcc gaagaactgt tgcatcgtcc gatattgaaa attatggatt gcttggaaac
    13621 gaagatttcc catccctttt tggaagagtg agaaaaaggt tgtggtgaac ttttccttct
    13681 gcacgatttt ttgtatatct gctgaaaatg tctgctcaga aaacacccgc aattcgctga
    13741 ttttttgagt ctgccattct gccgcttccc tttcgatttc ccgatttgtt tccatgattc
    13801 gctgataatc atcaacgact agtaaactat ttgaagggaa ataatccaag atcgtcacag
    13861 attcttggta aagaagatct gagtagaatt ttgcttcttc tgtcgggaca cctgcttgcc
    13921 agcttgaagt cagctgaccg aagtaatctt gcaaaaactc gcggtctgct tcttctgtgg
    13981 tcgcagcaag ccgtttttct agcatttttt gcatttgctc tattccatgt tctagatcct
    14041 ctggtccgta gacttgttca ctcgttgggg aaacccagat ttcttccgtt ttttcgattg
    14101 agcgttgtgt ttctgcttca aaataccgca tagaatctat ctctacatca aatagttcta
    14161 cacgaacagg ataggttgtc gttaacgggt aaatatcgat aatgcttcct cgaatactga
    14221 attctcccgg ttttgcgacc atctcttctc gtacatagcc catcaaagtc aattggcgag
    14281 gaagattttc cacgtctatc tcatcaccaa tatgccaatt caattgcgcc tgtagccaag
    14341 ttttcttatt cggcagtcgt ttgtgcaaag atgcgactgg caacaaatag atacctgctt
    14401 ttttttcttg aagtgcgtta agcgttgcaa ctctctcgcc tcgcgcttct ggagaagaaa
    14461 aggccatttc agcagaaagc acttcatcca ctggaaataa atggatctga tcttgcggaa
    14521 tcacatgttg cagatcttct gctaactgat tcgcataata caaatttggc atcagaataa
    14581 tcatgttttt atttgttttc ttaaacgctc gagcaaatag aagtgttttc gctgagcccg
    14641 ctaatcctgt gattagttgt ctaggcattt tttcttctaa attgctgacc cattctttta
    14701 cttgcggatc ttttccgact aattgaataa tatccattcc ttttcctcct tcctcaattg
    14761 aatcgattca tcgcatccac gaacgaatgc ccttctatga gatattcgac tgctttaaca
    14821 gattgacaca tgctttcttc tatcaaaggc tgattttctt tgctgaaagg actaagcaca
    14881 tgctggacta ctgttttttt cccttctggc ctgccgattc ccaccttgat tcgatcaaag
    14941 acattcgtat tcaaatggga aataatactt ttgataccgt tatgcccgcc ggcacttccc
    15001 ttttgacgga gccggatttt tcccactgcc agatccaaat catcgtagat tacgactaat
    15061 tcctctggat aaatcccaaa ataagtcatt agtgggccga ctgcacgacc agattcattc
    15121 atgaatgttt gtggtttgac taaaaggatc ttttctccat tatgaaaaaa ttcggcaact
    15181 tctgcttcga acggattttt tttgaaagta gcttgatgct cgcgtgcaat tttatccacg
    15241 accataaagc cgatattgtg ttttgtatat tggtatttgg tacctgggtt tcctaacccg
    15301 acgatcattt tcatttttgt tttccaccta attctaaaaa gcagcacaaa aggctggact
    15361 gctaattagc ggtccagtcc atcgagcttt tttctttttt acacttgtca atgcatagta
    15421 taacacaaaa caaggcgttt cggctaaaag agaatcgtta ataaatgata aagaaattaa
    15481 aaatttcttc aaagtttaat gaaataattg atatataaaa atagtgaaat aacgcacatg
    15541 taagctttta catataatac aaccttgtta tctgttatat tttatagcca aatagcgtga
    15601 catggggctt gagaaatgtt agcataagca tgggaaaaaa caaatcaatg gaaggattga
    15661 tactaaatga ctgcagctgc agaaaaaaaa gatcatcaaa aagttattct tgttggagac
    15721 ggcgccgtag gttctagcta tgcctttgct ctagttactc aaaatatcgc tcaagaagta
    15781 ggtattgtgg atatcaatac tgctaaaact gaaggagacg caattgactt gtctcacgct
    15841 ttggcattta cttcacctaa aaaaatctat tctgctactt acgcagacgc acatgatgct
    15901 gacttagttg ttattacagc tggcgcacca caaaaaccag gtgaaactcg tttagactta
    15961 gtaaacaaaa acctaaaaat caaccgcgat gttgtcacac aaatcgttga atcaggattt
    16021 aacggtatct ttttagttgc cgctaaccca gtagatattt tgacttactc aacttggaaa
    16081 ttctcaggtt tccctaaaga acgcgtaatc ggttcaggta cctctcttga ctcagctcgt
    16141 ttccgtcaag cacttgcgga attagttgac gttgatgcac gtaacgtcca tgcttacatc
    16201 ttaggtgaac acggagattc agaattccca gtttggtcac atgcaaatgt tgccggcttg
    16261 caaatctatg aatgggtaaa aaacaaccca gaaatcgatg aagaagctat ggttaaccta
    16321 ttctttggcg tacgtgacgc tgcttacaca attatcgaga aaaaaggcgc tactttctat
    16381 ggtatcgcag tagcattagc acgtatcact cgtgccatct tagatgatga aaatgctgtt
    16441 cttcctttat cagtattcat gaatggcgaa tatggattga acgacatcta cattggtgca
    16501 cctgctgtta tcaaccgtca aggtatccaa aaagtaatcg aaattccttt aagtgacagc
    16561 gagcaagacc gtatggctgc ttctgctaag caattaaaag aaattctaga tgaagctttt
    16621 gctaaactag acgctgaata atctttgaac atgacggaaa acatctattc ggtgttttcc
    16681 gtttttttat tttatcaccc ttatggtttc taaatttttt cataaagtag agataacctt
    16741 cgtcttttcc tctaaattat gctatcatga gaccgttcca ccctaaactt ttacttaagg
    16801 agttgtcgat atgacaagat cttcgcatcg aaaaaaatcg aaacatgttg ctttgctttc
    16861 agttcttggt gttctcttag tgctgatcag cttttatacc tatcgcagta catactacac
    16921 aaagcatttt ttgccgaata cagaaataaa tggaataaat gtcagcaatc taacagtcga
    16981 aaaagcaaat gaaaaattaa aagaagctta ttctgataaa aaactggcta ttaaagaaga
    17041 tggaaaagtc tggcaggaag taccaaaatc cgagctcggc tacaaagatg atttcacttc
    17101 ggagctttct ccgatcctaa atgaacaaaa cggatggact tggggaatga cttatgtctc
    17161 agcggcagaa aaacaagaaa tagatcctgt cagtcaaaac caccaaaaac tagacactac
    17221 cgttcagaca ttgacaacta agctgactga tttgaacaaa gaccggacac ctactactga
    17281 tgcaacgatc gaaaaagcag gtgactcttt cgttatcaag cctgaagtca atggaaatac
    17341 gatcgacgtc gatgctgcgg tcaaacaatt aaaaagtgcg gtaaactctg gtaaagatac
    17401 aattgaattg actgaattca aagaaaaacc aaaagtaaca tctgaagaca gctcactgaa
    17461 agaacaactc gcatccatga atgctatcgc aaatgtaaaa gctacttaca gtataaacgg
    17521 tgagactttc cagatcccat caagtgatat tatgagttgg ctgacctaca atgatggaaa
    17581 agtcgattta gatacagaac aagtacgtca atatgtaaca gatttaggca caaagtataa
    17641 cacaagtacg aatgacacaa aatttaaaag tacaaaaaga ggagaagtta cggtcccagt
    17701 tggcacttac agttggacga tccaaacaga cagtgagaca gaagcattga aaaaagcgat
    17761 tctggcaggc caagatttta ctcgttcacc cattgtgcaa ggtggcacaa ccgctgatca
    17821 tccattgatc gaagatacgt atatcgaagt tgatctggag aaccagcata tgtggtatta
    17881 caaagacgga aaagtggccc ttgaaacaga tatcgtttcc ggaaagccaa ctacacctac
    17941 acctgctgga gtcttctatg tttggaataa agaagaagat gcaactttaa aaggaacaaa
    18001 tgacgatgga acaccttacg agagtccggt aaactactgg atgccgattg actggacagg
    18061 tgttggtatc cacgattcag actggcaacc tgaatacggt ggagacttat ggaaaacccg
    18121 cggttcacac ggctgcatca acaccccacc aagtgtaatg aaggaattat tcgggatggt
    18181 cgaaaaagga acgcctgtat tggtcttcta acaaaaataa tatataaaaa gaaagcaccc
    18241 gtacgattgg tacaggtgct ttctttttat tatctaacaa tgtgctactt acatttcagc
    18301 agcagtaaag acttctgaaa catcatcatc gtcttctagc ttatctacta gacgttccaa
    18361 ttgctctttt tgttcctcat ttaaatcaat cgtcgtttgt ggaatcatcg tcaattctgc
    18421 ttgcgccaaa gtgaactctt tttccaactc atctcgtaca gcagcaaaat cttctggtgc
    18481 agtataaatc tcgaatactt caggagaagt ttgcagatct tctgcccccg cctctaatac
    18541 atcctcaaac atcgtctctt catcaacagt caacccatcg cgttcaatag cgatatatcc
    18601 tttgcggtca aacaagtaac tgactgaacc cgtttctccc agtgatccac cgtttcgagt
    18661 aaaagctact cggacatttg tcgctgtccg attacggtta tctgtcaatg catggactaa
    18721 gattcctact ccacccggac catatccttc atacgtgatt tcatcgtaat tcgtttcatc
    18781 tacggcactc gtagcctttt taatcgcccg ttccacattg tcgttaggca tatttgcagc
    18841 ttttgctttg tccacaacaa gccgcaaagc tggattcata gatggatcag gaccgcctgc
    18901 tttagctgcc atataaattt cacgagaaag tttctgaaag actttccctc gttttgcatc
    18961 ttgggcattt tttcttcctt ggatattatg ccatttacta tgtcctgaca tgatttttcc
    19021 tcccttgttc ttttgagaat acacttctat tctagtacta tttcacaaaa aaacaaagcc
    19081 acagccaatc tataaacgct tcttgaatgc cgcaaaaagc acacataata tcacagcagt
    19141 cagagcaccg atcgaatcca acatgacatc ttggaacata ggtgttcgtc caccggtcat
    19201 catctgatgg aattcgtcca aacctgcgta ccctgttgct gaaagccaag caaggatcgc
    19261 tgtcagccac caaattttta gcttggggaa aacacctaaa aacaagctgc ctcccaaaat
    19321 aaaataggta ccgaaatgtg cgcccttcct taaaaagaac tctaaaaatt tggcgtagcc
    19381 cagatggttg atactcacca tacttccacc ataattaaaa cgtatacccg ctaatgtgtc
    19441 tttaaatggc tgattaggca gccagctttc taatcgcgaa acttgtgatt gctgtccgta
    19501 agtttgagaa gaactgtaaa ataagattat aacgactatc agtgcgatta ctaaataaat
    19561 atttccgttt ttcatttgtt tttttgaaga attcataagt cacctctgta ttttatctta
    19621 gacttttttt gaagaaacaa caatcatttt catagagact tcttccataa aaaagtgcta
    19681 tacttaattt tgtattacaa tatgaaggga gttttttcat gagttattca ttaaattgga
    19741 atctagattc tattttttcc ggaggcagtc attctgatgc tttgaaccaa cggatgaagc
    19801 aattggaaga ccaaatgaac gagtattatc accgcgtaac aaaatggtca cctagttctg
    19861 acaatgcaga gcagttaaac gctattcttc aattgcaaga aacgattacg aatggtttta
    19921 cccaatgcag cagctatatt actgcattgc tttctgccaa tgtcaatgat tcagatgcaa
    19981 aggtcttatc aggcaagctt tacgcaatgc tgcctcgtct gcaatcagcc gaaactgtat
    20041 tgtcaaagaa atttgcagaa atatctgaca atgattggaa tcagatgctt tctcaatctt
    20101 cgtttgaaac aattgctttt cgtttaaacg agattcgtcg tgatggctca caattattat
    20161 cagaagctga agaaaacatt ataaatacgc tttctttaga tggtctaaat gcttggagca
    20221 gtcattatga tacgattgta gccagcattt cgattccctt cgaacaagat ggacaagtag
    20281 tcgaattatc tgctggacaa gccttcaata agatgatggg cgatccagat ccaaaagtca
    20341 gagaaacact tttcgcagca tgggaagaag cctggaaaga aaaggcaccc ctcttcacgg
    20401 atactttgaa tcatttagat ggttttagac tttctgacta tgaacttcat ggtgtcacag
    20461 attttctaca aaaaccatta gagtacaatc ggttgaagaa agaaacattg tctgtcatgt
    20521 gggatacaat ccagaaaaac aaacaacccc ttgttaatta cttgactaga aaagctaatc
    20581 ttttcggtaa agaaaaaatg gaatggcaag atcaagatgc acctattatt ttaggcgatt
    20641 tgaaagaaaa aacctttagt tttgacgaag cagcagcatt tattatcgaa aactttaaca
    20701 aattcagtcc aaaaatggct aaatttgccc aatctgcttt tgaaaagagc tggatcgaag
    20761 cagaagatcg tcctgggaaa cgtcctggtg gttattgtac tgagttacca gaaacaaaag
    20821 aatcaaggat ctttatgacc tacagtaatt ctgttaatga ggttgcgaca cttgctcatg
    20881 aactaggaca tgctttccat agcagcacga tgtgggattt accttcttta aatcgcgaat
    20941 atgccatgaa tgtcgcagaa acagcaagta cattcgcaga attgatcgta gccgatgcta
    21001 cattaaaagt cgcaaaaaca aaagaagaaa aaatcaattt gctagataca aaactgcaaa
    21061 atgctttagc tatgttcatg aatatccatt cccgattcat ttttgaaaat cgattctatg
    21121 aagcacggca agaaggatta gtctcagaag ataagatcac agagatgatg gttgaggcgc
    21181 aaaaagaagg ctatcaagga gctttagcta cttaccatcc atatttctgg gctgcaaaat
    21241 tgcatttctt tattgatgac gtaccgtttt ataacttccc atacacattt ggctacctct
    21301 ttagtttggg catctatgct tacgccaacc aaaagggagc aaattttgaa gaagaatata
    21361 tccatttact acgcgatact gcctcaatga cgacggaaga tcttgcgaag aaacatttga
    21421 atgtcgatct tacccaacca gatttttggc aagcaggtat cgacatggtg actgaagata
    21481 tccaaacgtt catggaattg acagaagaat ttatctgatt tctattttat cacaataaaa
    21541 aaagagttag tccagaatgg gctaactctt tttgatgtgc gccctgcatg ggtgaaaact
    21601 tggtggtgaa agtccactac aggcttggca gtaggaactg ttagcgaaag acaaggcggc
    21661 aatcgtgagg ttgtggctga aggaagtcgg acgcaaactc ctgaactgac gaacagaaac
    21721 tagatacaag gctgaattag gtcggataag tcttcatcac aagacaaagt ccaatactgc
    21781 ccgaaaccta tacagtaaat ctagcagtta catgggagga aagttttcac tcttaccggg
    21841 ggagatctca tgagggtgtt gtgggcgttg aatgaaaaca aaccagcaca acgatcatgg
    21901 cgacatgatg gtgaatcatg agaagtcagc cgaggtcata gtagtaacga cactgaccgt
    21961 tacgaaggac tgaacaataa gaatcttgaa taatttagga ggtgtgagaa gatgcgacga
    22021 ccgcagaaaa caagaaaact tggctatcat caaagagcta gtgtggaaca cgaagggtat
    22081 gatgaagtgc gcagcacttc ttgcggtgaa cagaggaaac aagatggtgt agaaaatctg
    22141 tttgggagaa ttctagatcg gaataatctc aatcaagcgt atcttcaagt agtcagaaat
    22201 aaaggggcgg ctggtgcaga tgggatgacc gtcgatcaac tgcttcctta cttgaaagag
    22261 caccgcgagg aattactatc caatttacgc caagggaact ataaacctca gcctgttttg
    22321 cgagtagaaa taccgaaacc aaacggcggt gtaagaaaac taggaattcc cacggttatt
    22381 gatcgtatgc ttcaacaggc aatcaatcaa gtgttacaac cgctatttga accagaattc
    22441 tcagataata gttatgggtt tcgcccaaat cgcagcgcac atgacgcagt gaagaaagca
    22501 aaatcttatt atgaacaagg ttaccgatat gtggtggaca ttgatatgaa agcctacttt
    22561 gacaccgtaa atcacgataa gctgatgtat ttcattgagc ggaaagtaaa ggacaaacaa
    22621 gtcctaaaac tgatccgtca atacttaatg agtggtgtaa tggaaaatgg actcatcaca
    22681 acaactgaag aaggaacgcc ccaaggaggc aatctctctc ccctgttaag taacatctac
    22741 ctgaacgaat ttgatcaagt gctagaaaat ctaggacata aatttgttcg atacgccgat
    22801 gactgcaata tctatgttag aagcaaacga gcaggacagc gtgtgatgaa gaaatccatt
    22861 caatttctcg aagaacaact aaagttgacg gtaaatcgag aaaagagtgc ggtaggaagt
    22921 ccattgaaac gaaagtttct tggattctgc attctgccaa caaagaaagg tattaaaatt
    22981 cgccctcacg caaaagcaaa acagcgtgtg aaaagcaagc tcaaacaact cactaaaaga
    23041 aaccgtggaa gaagtatcca acttatttta aaagaaatca agcaactaat gactggttgg
    23101 atcaactact atggaattgg tgagatgaaa gaattcatga gggagttaaa cggttggttg
    23161 aagcgaagaa tcagacaata tatttggaaa caatggaaga atccaagaac caaaagaaaa
    23221 aatctgattc gtttagggat cgataaagcg aaagcgtatg aatggtcaaa tacaagaaaa
    23281 ggtatttggt cagtctccaa aagtcatatt cttcatcgat cattaacaga caaagaactg
    23341 gcccgccaag gatatgagga catctccttg aagtaccagt tcgtacactc aacttattga
    23401 accgccgtat acgggtccgt acgtacggtg gtgtgagagg ggcggaaggt cagtt
//
LOCUS       DAZAFF010000042        22028 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000042 DAZAFF010000000
VERSION     DAZAFF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.042"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..328)
                     /locus_tag="ACG9BB_001531"
     CDS             complement(26..328)
                     /locus_tag="ACG9BB_001531"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2837476.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="ACG9BB_001532"
     CDS             complement(353..2446)
                     /locus_tag="ACG9BB_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN2837477.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="ACG9BB_001533"
     CDS             complement(2935..3699)
                     /locus_tag="ACG9BB_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFN2837478.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="ACG9BB_001534"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="ACG9BB_001534"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFN2837479.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="ACG9BB_001535"
     CDS             complement(4704..5780)
                     /locus_tag="ACG9BB_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFN2837480.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="ACG9BB_001536"
     CDS             complement(5804..6397)
                     /locus_tag="ACG9BB_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFN2837481.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="ACG9BB_001537"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="ACG9BB_001537"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFN2837482.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="ACG9BB_001538"
     CDS             complement(8067..8366)
                     /locus_tag="ACG9BB_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFN2837483.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10119)
                     /gene="mdcA"
                     /locus_tag="ACG9BB_001539"
                     /pseudo
     CDS             complement(8468..10119)
                     /gene="mdcA"
                     /locus_tag="ACG9BB_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            complement(10126..11037)
                     /locus_tag="ACG9BB_001540"
     CDS             complement(10126..11037)
                     /locus_tag="ACG9BB_001540"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFN2837484.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11157..12014
                     /locus_tag="ACG9BB_001541"
     CDS             11157..12014
                     /locus_tag="ACG9BB_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN2837485.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12201..12563)
                     /locus_tag="ACG9BB_001542"
     CDS             complement(12201..12563)
                     /locus_tag="ACG9BB_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFN2837486.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(12752..12934)
                     /locus_tag="ACG9BB_001543"
     CDS             complement(12752..12934)
                     /locus_tag="ACG9BB_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837487.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            12909..14348
                     /locus_tag="ACG9BB_001544"
     CDS             12909..14348
                     /locus_tag="ACG9BB_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2837488.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14355..16907)
                     /locus_tag="ACG9BB_001545"
     CDS             complement(14355..16907)
                     /locus_tag="ACG9BB_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFN2837489.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17021..18352)
                     /locus_tag="ACG9BB_001546"
     CDS             complement(17021..18352)
                     /locus_tag="ACG9BB_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN2837490.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18318..19796)
                     /locus_tag="ACG9BB_001547"
     CDS             complement(18318..19796)
                     /locus_tag="ACG9BB_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN2837491.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20101..21936)
                     /gene="tdc"
                     /locus_tag="ACG9BB_001548"
     CDS             complement(20101..21936)
                     /gene="tdc"
                     /locus_tag="ACG9BB_001548"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFN2837492.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT         6415 a         4702 c         3775 g         7136 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tgcattggct aagcctgctt gtttttgaac
     8821 agctagtgca tctaattctt cgacaaagac tggtttttta tttgaaccat atgtttcaac
     8881 catttgaaca actaattttt ggcctttccc taatggatca tcattgtcgc gaagtgattg
     8941 ccatgctggt gtagaatgac gacgaccgcc aggattatgt cccatgttag gcgcaccgcc
     9001 aaaaccagat aaacgtcctt ttgttacggt agaagagttt ccttcgtaat ctaattgcaa
     9061 ggttgaaccg ataaacatat cgatggcata ttgtcctgca gcttgtgata atgttcggtt
     9121 actgcgcatc gtgccgtctt taccgacaaa aaagacatct gggcgagcag caatatattt
     9181 ttccatacca acttcgccac caaaactatg gatgctttca acccaacctg attcgatggc
     9241 tggaatcatc gttggatgtg ggttcaatac ccagttcttc gcgattttcc cttttaaccc
     9301 tagtgattca ccataagttg gcagtaacaa ttcgatagct gctgtattga atccaatacc
     9361 gtgattcaaa gattgtacgc cgtgttctgc gtagattcct ttgattgcca tcattgccat
     9421 tagaatttgc aattctgtga tgttttgagg atctcgtgtg aataaagctt ctaattgata
     9481 aggtttatct gctttcacga caacgtctac ccagtcacca gggatatcta cacgtggtaa
     9541 agaatcgaca acttcgttaa cttgaacgat cacgatacca tctttgaaag ctgcagcttc
     9601 gatgattgtt ggtgtttctt ccgtattgaa acctgtgtac aagttgccgt ctttatctgc
     9661 tttatctgca gctactaaaa caacatttgg aatcaaatcg atgaataagc gtccataaag
     9721 ttccaaataa gtatggatat cacctaattt cattgtgccg tcttcaatca tttgggcgac
     9781 acgcaagctt tgtgcaccag cataagagaa atcgatcttg cttgcgattc ctttttcaaa
     9841 aatattcaaa tgttctggtc gtgagatact agacatgatc aaatgcagat cattcacttt
     9901 ttctgcatct actttttcaa gactttctga aagaaaactt gcttgttttt gattgtcgcc
     9961 ttctaataca actttgtcgc cgggagcaat cagtttttcc attacttcta cgattttttc
    10021 agtttctaca tacttgccat tgagcaagtt gctgattttt tccaatcgct gcgctttttt
    10081 ttcgcgttta gaagcccagt tgcggtttgg tttttccata attagtcatc tccatttttg
    10141 atatagaaac gtggcgtcat caataccatg ttggtctaga ttgatcgtac ggatcccttt
    10201 atctacacca aattttgtat gaatcagctt tgtcaaagta ttgccggggg gaaattcaat
    10261 cgtcagatcc attcctaatt cttttgccaa gtccatcatt tggttccact gcacagggaa
    10321 agctaggttt tccaacaaat ctttacgaat ctcttctgcg tcctgagtgg ctcttccatc
    10381 cgtatttttt atatacaaac atctagcatc agatagatgg acagactgca tatacggctt
    10441 gaatttcttg accgtatcat tcatcaagag acagtgagat ggcacaggga ctttcagcag
    10501 cttcgctgtt tgcgcttggt gccgtttggc ttctgccaac gtaacggata aaccttcgat
    10561 actcccagat aacgtatgtt gcatcggaca attttcattt gacagataaa ctggatggtc
    10621 aggatcaaat gtttttgcta caatttcctc agcttcgctt ctcgtcacac ctactactac
    10681 gcccattgca taaccgcttg gataagcttc tttcattaag actgcgcggt gatagactag
    10741 ttccgctgca ttctcaaatc tcaaagatcg acaagcaaca gctgcacaaa aagcaccaat
    10801 cgaatgtcct gctgcaatat caggctgaat accgtttttt tccatttcat ctaaataaaa
    10861 tactgctttt gtcagtaatg ctagctggat aaaaaccgta tcttgatagt tttcttcttt
    10921 atcaactaac cgataccccg tcacattttc taaacgttcc atgtacattt cagggatatc
    10981 cgctaacatc cctaacttct gactgccttg tccaggaaat aagaaacctg tactcatgag
    11041 cctcgcctcc tcagcactta ctttttccac tgctatctta gtcaatttct gctataataa
    11101 aaaatgccaa aaaaagaaaa aaccataaca aaaaggtatg gatgggatgg ataattatga
    11161 aattgcttca tctgcagtat tttaaaaaag tattcgaaac aaaaaatgtt acccaagcag
    11221 ccaaagaatt atttatttct caacctgcct tgagccgtgc gatcaaacat ttggaaaacg
    11281 aattaggtgt ccctttgttt taccatagcg gcagaaacat tgagacgacc gtttatgcag
    11341 aagaatttta tccatatgca gtcaagacat tagacacatt agaagaagga atcaaattat
    11401 tgtcttcggt caatgagaag atcgttactt ccgtcatatt gtatttagag gtagcctctg
    11461 tttctattcc gaacttggtc cgaattttta tggaaaagca tcctgatatc caattaacga
    11521 tcatgcaaca tcatttacca gatgatatta ccgatcctgt actgtacatc acttcggaga
    11581 tgaagcctgg catgaccaat attcctatca tgaaagaacc tgtttatgtc gccattccta
    11641 agaaacatcc tcttgctaaa aaagaacatc tgcaattaga agatatccaa cgcaccccca
    11701 tcttgatgct gtcaaagagc aacgccttcc gtcatacact ggatgcagca cttgaagcga
    11761 aaagtatcga attgacgatc agttcgacga cagatgatcc tgcgacatta cgttctattg
    11821 tcaagcaagg attagctgtt agctttttcc caaaagtttc ttggtcttac gataaaaatg
    11881 atcctttcgt cttgcgggaa atcaaagatt tgcccttgac acgaacaatt tatttatctt
    11941 ctagcttcac agaagatcat cctttgatca aaatgattgc aaaaacgatg cgggaatttt
    12001 atttaggaaa ataaaaaaaa aagagctggg acaaaattct tgtcgcagct cttttttcga
    12061 acaaacggtg ttccagaagt aatttcttag aaaataagcc gaaatttccc aaaaatttga
    12121 gaagcaattt tcgtaaattc cttcttattt cttgaagcta aacatttctg tcacaacctc
    12181 ttctctattg tcctagacat tcattgcgtt gatttaaaac ttccatcaat caaatctgct
    12241 agttcatcta gcttttctat tttacgtaga tcaatagaga tccaatgttc tttattcatg
    12301 tgataggctg gaaaataccc ctcttttcct cgcaaaattc cgccaagttc aggatcgacc
    12361 ttcaaatcaa gaatattgat gatcctatgg tcacttgcat catcatagat tttttcttct
    12421 ggaacagaca tcaggacccc aaaccatttt ttgctttttt gatgtctgaa tacgcaataa
    12481 tcaggaaatt ttttaaaaat atattctgga gacaaattgt atgtttcatt gatgtgatcg
    12541 attagttctt ttttcgttag catctgcttc catctctttc ttattgattc gctttattca
    12601 aagataccac gtaagaatag aaaaatgaat acaagtagtt gctaatcaaa tacttcgata
    12661 tttttctttt ttatcaactc ttaaatgttc aatccgcctg tgttggtctt gctaccatct
    12721 actattaaaa tgtttattat tgtttgccca cctatttttt gattttagtg gtatcatttt
    12781 tcaagttcat gatgaaattc ttatcatcag taaatgctcc agaaaaataa acaatgattg
    12841 aaaaatcagt tgaaacttga ccaccgttta ttttctccgt attcttagaa tagaagggag
    12901 tctgattgat gtattcacta atgaaaaaaa tcattaccga aaaagatatt cgacgtcatg
    12961 tttctttagt agaacaattg ctgaatcata caaagataac cgtaaatgaa ttagctgaaa
    13021 tcatagggac aaccgaacga acgatttttt ctgaccttca atctattcgc tctcagctgc
    13081 ccgaaggctg ggacatcatc agcgatcaag caggaatcag tttgcaaaac cagcaaaatt
    13141 tattaacaaa tgacctttgg gagatttttt tcaaacaatc agtcagtgtc gaactgctga
    13201 aaaatctgct ttttacaaaa aaagttgctg tccctgattt tctagccgac catgggcttt
    13261 cttatgggac attgaaaaga catgtgacta aaatcaatca gcgtttagca agttacgact
    13321 tgcagatcga tttgacgaaa tatacagctt gtattcttgg aaaagagcgt gcgattcgta
    13381 ctttttatca tcgattgctg attcctttca ctcataacaa ctattttttc gaggattatt
    13441 cgatccatga atcccattat tttcaatttt taagaaacct cagtcaaact gaactagcgg
    13501 ttgaaacaga agagattttc ggaacatgtt ggttctttat caatacgatc cgtataaaag
    13561 cgaattgccg attagattct tctatccata ccaacagcac actctcctcc ctgtatgact
    13621 ctgcattgaa aaaattgtat ttaacggaag gtatttatct taaagacact gagctttctt
    13681 ttgcttcttt ctgctttctg gaaagttgga attacaacaa taactatgga caagaaaccg
    13741 ctcgctgcct gcatcattct ccatttttag aggtcctcga aacgtttgta gaggaactag
    13801 cttcagagct gtctcttgat caactaaaaa aaacatctct gactgataac ttggctctcc
    13861 tcatattaaa atatcatgaa tcgccgatat tgattgaagc attggaccgt caataccatc
    13921 gttttttaga aacatatgat cagcaatatg cacacttgta tcaacagaaa gagaatttac
    13981 tgaacgatct aaaaaaagaa atagaaatca atgaaccaaa ttactttctc caactcttgt
    14041 ctctactgat ccaacaaacg attttttcta tcaaaccgaa tttgttcaat gtctatttta
    14101 ttttccaagg agaaccttca tggaaggcgt tcttgcagca ggaattaaaa gattacttag
    14161 gaaaacgtgt ctgcttctta cctatcgaac taaccgatct ttccactatt tcttttgaaa
    14221 aaacagatat cttgatttcc aatttccctc tagattctct agatatccct attgtataca
    14281 tttcttctat tccaacgaaa aatgagctca atcgtttgac tgaactcact ttcaaaactt
    14341 ttttatagta acggctagcg actgtattct ctagctgttt tttctttttt gcgaaacagt
    14401 ttgttcatga tccctttttc tatttctaca actaagaata gaagaatcac atttgctcca
    14461 atcaataact gttgtgtcat cgttaaactt actgtaccga tcagctgctg gccgactggt
    14521 agaaaaataa ccatcccttg caataaagca aggataccta atgaaagaaa aagcgctttg
    14581 ttttgcaaaa gaccttggtt caatgacggc ttggacaact ctctgcaatt gatcatgtaa
    14641 acagcttgag ctaagacgat attttgcaga agaatggttt gctgtaagct ttgcccttca
    14701 aactgcatag ccaataaata ggcaggaacc atgatcagta gggagacata gatgatccga
    14761 aacaaatgat aaccagatag gattccttgc tttggatctc ttggtgggcg cttcatgatt
    14821 tctggatctg gcgactcgaa ccctagtgca taagataaag tgattgtcgt taccatattg
    14881 acccataaaa tctgtactgg ggacaaaggt agcgggcgat tcagcataag tgcccaaatc
    14941 aagatcaatc cttgagctaa tgcagttggc aagaagaaat tgatggtttt ttgcaaatta
    15001 tccaaaatcc gccggccctc ttttacggct tttgcaatcg tatggaaatt atcatctcct
    15061 aaaaccatat cagctgcctg tttgcttact tcactacctt tgattcccat agcaattcct
    15121 acatcggatc ttttcaaagc aggtgcatca tttacgccgt ctccggtcat tccgacgatt
    15181 tctccatttt tctgcaaagc tttaacaatt cgcagtttat gttctggtgt cgttcgagca
    15241 aagacatcga ctattggcac ttgctgaatc aactcttcat cagacataag atccaaatcg
    15301 atcccttcta aaactttggc agtatgtttt aagccaattt gttccgcaat cgcttttgct
    15361 gtgtctttgt gatcccctgt aatcattttc actgagatcc ctgcttgttg gcattccttt
    15421 acagctttga ctgcactttc ttttggcgga tcgatcaatc cggcgatccc tgcaaaagtt
    15481 aattgggtta aagtctcatg cgtgagttcc tgtttcgaat ctactttttt ataagcaaat
    15541 cctaaaacac gctgaccttt tctcgcaaat tctgcagctg tctgactcca tgcttcttgt
    15601 tctgcagaag aaagagttga tttagcaaat aatacttctg gtgcaccttt gacgtaaatc
    15661 accgctcctt tttcctccgc atgcatcgtt gccatatatt tgtattcaga actgaacgga
    15721 atctttgctt ctattttttt caaaggtaga ttggctgttt ctgtgtgtcg aatcagcgca
    15781 acttctgtcg gattgccact taaattttct gttttctgtt cttcttttgt ttttaattcc
    15841 tgacagtttt tcataatgga caacacgcag gcttcttctt ttgcagctac atccacaact
    15901 gtcatttcat tttttgtcaa tgttcctgtc ttatctgaac agatcactgt catagaacct
    15961 aatgtttcta ccgaaggaag tcctttgatg atcgcctttt cttttgccat ttcatgtact
    16021 ccaacggata gaatcatggt caaaacagct ggcaatcctt cgggtaccat tgacaccacc
    16081 aaagcaatcg tagccgaaag aaggatatgc caatccatac catatcgaaa tgtcgtaaaa
    16141 ataactaaga ataggctcaa acaaagaatg ccttgaaaaa tttgtttgtt taatcggttg
    16201 atttttttaa tcagaggcgt cgtttgttgt tcgaccgatt gcaatgcttg attgatttta
    16261 ccgatttctg tcgtgtttcc tgtttctaca acgacaccga ctgcagaacc agattgtaca
    16321 agcgttcctg aaaaacccat atttttctgg tcgccgatcg attcttcttc tgtcaaaacg
    16381 gcatgacttt tttcagatgc ttctgattct cccgtcaaaa tcgattcttc cactaataaa
    16441 tcatgtgctt cgattaatcg gatatctgct ggaacgacat ctccggcacg taagacaatg
    16501 acatctcctt ttaccagttc tttcgcaggg attttttctt gcatcccgtt tgatagtact
    16561 atcgcttctt gtcccatcag ctgcttcaaa ccattcaatg attcttccgc ttttctttct
    16621 tgccagtaac taacgagtcc attgacgatc actacgaata aaataatact tccttcaata
    16681 tattcactag aagcaaattt caaaaaagag gcaatgatca acacgatcat cagcagatca
    16741 gtaaaatgct ttgctatttt tttccatcgt tttgtctgct gccttgcttt tatttcatta
    16801 tatccttgat cagtcaggcg tttctgtcgt tcttcttgcg acaagccttc tgttgatgtt
    16861 tgatagtttt tcaatacatc ttctaccgtc aatctgtacc agttcatcct atcccctcct
    16921 gtttaattga tactgttatt atacgcaggg aagttttttc aggcgtatcg attatttcag
    16981 gggggtctga aataattcgt tttgcaaaat gtatctgggc ctaactgata tttctattgc
    17041 ttattctttt atatacaaaa tttaagaata caaaccagat aaccaataaa aaaggaataa
    17101 agcgtgtcgt gctttcgaat aacaagctga aagcaattga agtaaaaaac accaacaaag
    17161 caagatcttt atatctttgt gtagtgttcc gtttcttttc tttaataaat tgaacatgtg
    17221 cacacaagat cgaagaccac acaaatagaa aacaactcgt tgtcatgcct gccaaaagct
    17281 gaaaaaacaa tcgactatca gctacaaaaa aatgtagaca aagaccggca aaaaataaca
    17341 ttcctgtaat taaaataccg tgtaaaggta cgcctttctt agaaagcgca caaaaccaat
    17401 ggggcaaact tttttctttt gctgtttgat ataaaatcct gctagtactg tagactgcac
    17461 tatttgctga ggacaaagaa gcaaccaaaa tcattagatt gacaattgct gctccgcctt
    17521 ggcaatggat cacttgcagc atagtcacta aaggactttc ttttaaatct aaacaatgcc
    17581 atggaacaac aaataaaatc gacaaaatcg ttcctatata gaaaaagaga atacgccaag
    17641 gcagacagtt gattgctttt ttcaaattct tttgcggatc acttgtctcg ccaacggtta
    17701 agccgatcat ttcaattcca gcaaaactga ataaaaccat aggaaatgag actaccattc
    17761 cagcaaagcc aaacggtagt aattcatcga tctgtccaaa attttctttg attccgatag
    17821 actgggtatg aaaatctgag acactcaatc ctatacccaa taagataaaa aggaaaatcc
    17881 ccaagatctt gatgattgag aaaaaagatt ccaattcacc aaaccacttg actgcaacaa
    17941 ggttgactgc cattaagatc attagtgcgc tacctccgct tatcaaagct ggtacatgta
    18001 caaaccagaa atggatatac aatcctattg ctgttaattc cgcaagtcca gctccaagcc
    18061 agcaaagcca ataagtccaa ttgacaaaaa atgcccactt tttgcctaaa taatagtcaa
    18121 cgaaatcact gatagagtta aatgtactat ttgctaaaaa taaatcaccc atggcccgca
    18181 tcaaaccata aatagccaaa ccgcatatca aataaacgag aataacagct ggccctgctg
    18241 atgcaattgc ctttgctgaa cctaaaaata atcctgttcc aattgcgcca cctaatgcga
    18301 ttaactggat atttcgatta gttagagtcc gtttcattgc cggtttttcc atcaagttca
    18361 cctttttctt tttcaagcag catggcgact tcctgttccc aagaaggttt ctttattttt
    18421 tcaatacaga gtggtactac aaacaaaatg acactgatgg ctgtgactgc aattagattg
    18481 atccatgttc cagctttcat cgtcagtccg acagaaaatc cgattgccgc aaataaaaca
    18541 ataacgacag tccaaactcc aagattgctc tttttctctt tttttccaat tctaaatggt
    18601 cgattcaact ttgcatgtgt ataacgcaat ctcaaaactg cgatccccat gatgacatag
    18661 acgatacagt agataacagt agttgaattt acgagcatca aaaatgcttg attgacgcca
    18721 ggaatcagca aataaaaaag tgcaaagatt gagataatga cagactgagt caaaataacg
    18781 gatttggata cgtcaaattg attcgtcttc caaaaattga attttggtgg atacgcacct
    18841 tttctagcac tttctgtcac tgtctttgct ggtcctgagg cccaagcaga taattggact
    18901 gctacaccaa tgaagacaag taaactaaac agattcacaa taagatgtgg caaaccaaga
    18961 atttggcaat aaatcgaaat agattgcgcg atattattca attccatttg tccttttggt
    19021 acgacatttg ctacaactaa agcattcaaa atattgacga aaaatataaa aattaacgcg
    19081 gcaaatactc cgcgaatata cgttttaact ggattttcca atcgagtgat atacacagag
    19141 gacatttcga taccagtaaa aataaacatg atcggcgcaa aataaacaaa tgaggatgca
    19201 gtcgtagtat ctggtattaa tttgtcccat gaaaaactgc ctaatgttcc ggttggtacg
    19261 atcccaactt tgatccacgc agcaaaccct aataagagca tcatcgtcaa aggcacgtac
    19321 aatcctaacc aaacaccgac ttttccgccg atttttgcca tatcaaattt caaattcaaa
    19381 aaagtgatga tccaataaac gactaaaatg acaactaatg taaatttgct gttcaaacca
    19441 agcggcacat tatcgataat attaccaaac aacggagcga gtgctgaagc gaccatcacc
    19501 atcccaggaa acatttggac ccataacagc catgagacaa cgaaccccca tttttttcct
    19561 agagcattcg atacccaaag ttctggacca cctttttggg gaaacattcc agcaaattca
    19621 ccagagatca atgagattgg aaaagcgtac aataatactg caaccagcat gtaaaaaatc
    19681 atcgtccagc tcgttgctgc tacatctgga atatttcgta cacttgctac aagggcgcag
    19741 gtcattccga taaaggtcat ggtcgtgatt tgctttgatg aaccagtatc tttcatcttt
    19801 attccttctt tcaatagcga catatgtttt tgttttcttt tatttttgtt caagaacaga
    19861 aatgacaagg tttcatcgat tcagtacaaa acccagtatc accgaaacat ccattgaacg
    19921 atcttagtga actgggtttg atacatgaaa cgaacgatcc aacttataga acgttttata
    19981 aaaaagaata tcttggcgag gaatatatga aaaacagggg gaaatacaat tgttttgatg
    20041 aagcaaataa ttgtctgttc tccatatgtc tcttgttatt ctttcacaca aaaatcagtt
    20101 ctattggatg atttcgttta gtttcttttc cattgctttg gcgatttttt ttgtataaaa
    20161 attgaaaatt cgatcatcat tcaaataagg agtcatgata gccgcacgta gcagagtgat
    20221 tttctgttct tttttccatt cttctttcgt tagacccatt ctttcaacaa aacgaatcgg
    20281 agaatcaccg taatcttctt gtgtaaatgt agtatgagaa gtaataaacc gttcaccata
    20341 aacatcgcca tccatataag aagaccgatc gaacattttt tcatttaatt cattgatagc
    20401 gttcaaatct gtacagcctt gttctttgaa tacccaattg accatattaa aatcaggatg
    20461 attcaatgga tagacttcga ttgtttttcc tttaacagta aaagtcagat gatcgagaaa
    20521 ttctctgaat ctttgagctg cctcgattga agccccaatc aattgaccat atccggtcac
    20581 attcaatggc aatacacgat gtgctgtcca gactgcggcg gcggttgctc cagcttttga
    20641 tccttccaaa atataagctc ctaacatatc tggagccttt actgattttt caaacacata
    20701 tggcgcgaaa taagaaatga tatttctcat atttttatgt ttgattacga ttcctccagc
    20761 agcataaggg acatagccca tcttatgagg atctatcgtg acagaatccg cttctgaaat
    20821 agctctgaac ccttcatata cctctttgtc aatcgttacg cagtggtgga aaacatgatg
    20881 ttcttcaaag aattcagcta aggaagcata cggcacaaat tctcctgctt cgtttagaaa
    20941 cagtgaacgc gcatagccgc catatgcggc atcaacatgt aaatagaaat agatcccttc
    21001 atcttttaac ctttctctta attggacgat tttatctaca ctgtctactt gaccttcttc
    21061 tgtcgttcct acgacagcaa ccacacctag aattgggatt ttttgtccag ctaattcacg
    21121 aattgttttt tccaaagcat taatatccat tcgataatcc tcttgaactg ggatagcaac
    21181 catctgatct aagccgactc cgcaaatatc taacgccttc atccaagaat agtgttttgt
    21241 ttgagggacc agccatttcc ctaatctttg gatatttttt ccgctacggg aagaagctgc
    21301 tttgacctca tccatttctt cgtccgtaaa gctttcggtc atttccaaaa tttcttcaac
    21361 agataaattc aaaagttccc actcagacat ttttttcact ttttcaggca gcacttcctt
    21421 gacagctaaa ggaatcgatt tgatgcaacg ggcgtaccat aatccttcaa gattagcaat
    21481 agaaccatct gctgcaatgt gtccccatcc gtctgtcata ttgaataatg aagcaaaatc
    21541 ctgtcctact tctgcttcca tttgcgaagt tgccatagaa gattccaatg caacattatt
    21601 tgggttccac agcatcgcat agttgtaagc taataaagca ggcatcaatg tttcagcatt
    21661 catttgtccc cagtaacggc ctgctgaatg ccatgggatc gatcctgcgc gtaatcgctg
    21721 actgacttca tccaagacct ctatcatatg gtcgcgggtg gctagataat ctggagtgag
    21781 cttatctcct tcgctaattg caggcatatc tgaaggaata taattttctc tccatccaag
    21841 atgttcatct accatcttgt tcaacaacat tttataaact ggtccatttt cagctttgtc
    21901 gcctataaag acggccttga tatccatatc tttcattttt cttccctcca tattttgttg
    21961 gtcaatgtct tgcagataca tgtgtttatc tgtaacacct cttcactata gcgccaaaaa
    22021 tgagaatt
//
LOCUS       DAZAFF010000043        21589 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000043 DAZAFF010000000
VERSION     DAZAFF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.043"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1160)
                     /locus_tag="ACG9BB_001549"
     CDS             complement(<1..1160)
                     /locus_tag="ACG9BB_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2837493.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSL"
     gene            1417..4806
                     /locus_tag="ACG9BB_001550"
     CDS             1417..4806
                     /locus_tag="ACG9BB_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN2837494.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            4809..6230
                     /locus_tag="ACG9BB_001551"
     CDS             4809..6230
                     /locus_tag="ACG9BB_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN2837495.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            6227..8104
                     /locus_tag="ACG9BB_001552"
     CDS             6227..8104
                     /locus_tag="ACG9BB_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN2837496.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            8196..9038
                     /locus_tag="ACG9BB_001553"
     CDS             8196..9038
                     /locus_tag="ACG9BB_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN2837497.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(9190..10065)
                     /locus_tag="ACG9BB_001554"
     CDS             complement(9190..10065)
                     /locus_tag="ACG9BB_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN2837498.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            10272..11303
                     /locus_tag="ACG9BB_001555"
     CDS             10272..11303
                     /locus_tag="ACG9BB_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN2837499.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11392..11697
                     /locus_tag="ACG9BB_001556"
     CDS             11392..11697
                     /locus_tag="ACG9BB_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFN2837500.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            11876..13891
                     /locus_tag="ACG9BB_001557"
     CDS             11876..13891
                     /locus_tag="ACG9BB_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN2837501.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            13893..14177
                     /locus_tag="ACG9BB_001558"
     CDS             13893..14177
                     /locus_tag="ACG9BB_001558"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2837502.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            14203..15576
                     /locus_tag="ACG9BB_001559"
     CDS             14203..15576
                     /locus_tag="ACG9BB_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN2837503.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(15659..17674)
                     /locus_tag="ACG9BB_001560"
     CDS             complement(15659..17674)
                     /locus_tag="ACG9BB_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFN2837504.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(17696..18910)
                     /locus_tag="ACG9BB_001561"
     CDS             complement(17696..18910)
                     /locus_tag="ACG9BB_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2837505.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            19131..20075
                     /locus_tag="ACG9BB_001562"
     CDS             19131..20075
                     /locus_tag="ACG9BB_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFN2837506.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            20371..21315
                     /locus_tag="ACG9BB_001563"
     CDS             20371..21315
                     /locus_tag="ACG9BB_001563"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFN2837507.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         7344 a         3955 c         4070 g         6220 t
ORIGIN      
        1 agacttaagt cgctgataat cagtattttt ttatcagaag tataatggtg aatttcagga
       61 ttaccgataa gcatccaaaa gatatagcta gataaataat aaatcagatt ctgattcagt
      121 agagtggaat aaaattgaag ttctgcacga aatccagcat atagacgtat ctggcggtgt
      181 tgcatctgct gtgcaaagta agttgcacgg atcgtcaaat gagtcgtatc gtatgggaaa
      241 atacgggaga aaaaataaat ttgcgttaat aattgaacaa catattcttc gtttttttct
      301 ttgcaatcag cggatgaaaa cgaacagtag gacatatttt ttcgtatctg ctcactgatt
      361 tcattttttt ctttctccga aaattctgaa aaaatcgtat tgacaaaata gttcactact
      421 tttatatagc tgctgtactt caattttcct aagagatttt tcaagattaa gaagtctttt
      481 ttatcgatat ttatcaactg tacataagag tctccagctt tccattcaaa tggagttcct
      541 tgggattctc tcatgagatg cacaacaaaa tacataataa agaaagattg ctcatatggg
      601 ctttcttcct gaaacaaatt catctgacaa ttattcttga ccataatttc tttgatcttc
      661 tttttttcga ttggaaatgg caacgtgcgt cttccgtagt aacttaaaaa gaaaaaggta
      721 acaaagatac gcaactggag ctcactttta aggctagtta actgaaaacc ttggtcaacg
      781 atcactttta ataaatagct ttctaaaatt tgattgcatt gtttcaactt acgtgtaaaa
      841 gaagaccggc tgatggaaat tttttcacaa taatactcaa tcgttttacc cggattgaca
      901 ataatctcat ttaataaacg actgtttgta gaactttgcg acatttccat cagaacattt
      961 aaataagctg gtacattcaa atgttcaaga cgatacccca atttttgtga agattgaatc
     1021 gttacatccg ttccccagcg gattttgatc tcttcgatat accttacaaa tgtagattct
     1081 gaactaccaa ttcgtttggc aagttctttt ttagtcatcc acttgtcaga aatggacaga
     1141 attcgaatga ttttttccat ctttctatag tttccttcca acaacctgat catatgtttt
     1201 tctccctcaa cccttttctt tcaatgaaac gctcattaat cgaaggaatc aacttcataa
     1261 atgaaaccaa ttggtatgaa aattggtgtg attcccttta gcgcccttcg tataataatt
     1321 atcatattaa aataaataaa attatgtaac gcctgtttat tttaataatt ttcactattc
     1381 acgtcaaaac agaaaaaaag aaaaaggagg ggaaaaatga cgaccacagg gaagaaactg
     1441 aaagttattt tcatgctgat aatattgagt ttatcaaact ttgtgccatt atctgcaata
     1501 gcagacacta cagatgatcc aacagtttta gaaacaattt cagctgaagt catttcggat
     1561 cagtctggaa aaaaagcact gaacatcaag ctaaatgcga ataacaccag tgctgaaaag
     1621 atagaaaaag aaattggtct agtcgaaaat tacttaagtg atgtggaaag aaaagaagga
     1681 gatggctatg cttatcaggt aaatagcggg aaaattacgt tggaaatctc atcaaacact
     1741 aaacaaacta tcgatctgag ttttccaatc gatccagcac tttaccacag ccaggcaaac
     1801 aagctgatcg tcgataataa agaatatgac attattgatg agacagaaaa taagaaagat
     1861 acagatgtgt cagtaccaaa gccagacgaa atagaagaag aatcatcaaa agaaaacgaa
     1921 aattctgtca gcccatttac attgcctaca ttatccttgc cagctgtgag tgtgccatct
     1981 aatcaaacga ttcctacaga atatacaaca gatgatcagg gcacttatcc taaagccagt
     2041 tggcaaccta caggaaatac aaatgttctt gatcatcaag gcaataaaaa cggaacaaat
     2101 caatgggatg gtataaattc ttggaatgga gatcctaatg atcggaccca ttcgtatatc
     2161 gaatatggag gaaccggtaa tcaagcagac tatgcgatac gaaagtatgc aaaggaaaca
     2221 agtacacccg gattgtttga tgtttatttg aatgctcgtg gaaatgtaca aaaagatatc
     2281 acgcctcttg atctcgtatt ggtcgtagac tggtcaggaa gtatgaacga caataatcgg
     2341 atcggtgaag taaagattgg tgtcgatcgt tttgtcgata ctttagcaga tagcggtatc
     2401 acagacaaaa tcaatatggg atatgtcggc tactcaagcg aaggatatag ctacagtaac
     2461 ggtgcagtac agatgggttc atttgattca gtgaaaaatc aagtaaaatc cattacacct
     2521 tcacggacaa atggtggtac ttttacacaa aaagcactaa gagatgcagg aagcatgcta
     2581 tccgttccaa atggacataa aaaagtgatc gttttgctga cggatggtgt accaacattt
     2641 tcctataaag tacagcgggt acacgcacaa tcaagcagca attattacgg aactcagttt
     2701 tctaatacgc aagatcggcc gggaaatact tctctaatct caagaatcta tgatgcacct
     2761 gaccaaaaca atctatccag aagaatcgac agtacgttta tcgcaaccat cggagaagcg
     2821 atggcactca aagaacgagg aatcgaaata catggtcttg gcatccaact tcaaagcgat
     2881 ccggcagctg gtctctcaaa agcagaagta gagtctcgta tgcgacaaat ggtttcatca
     2941 gatgaaaaag gcgatcttta ctatgaatca gctgatcatg caacagatat ctctgaatac
     3001 ctagccaaaa aagctgtaca gatctcagca actgtaagca atggacaaat aaatgatcca
     3061 atcgcagaac cattcattta tcagcctggt acactttcag tcaagagtgt ggggacaagt
     3121 cctacaacgg tcactccatc tatttccata gaaggaaata ccatcaagag caatcagatc
     3181 tatttaggaa aagaccaaga aatccaaatc cattaccaag tgagaatcca aacagaaaat
     3241 gaggacttcc atccaaattt ctggtatcaa atgaacggca ggacaacttt ccagccaaac
     3301 attgatacca atgaattagc tgaattcggt ataccatctg ctaaagctcc cggagtcagt
     3361 cttcacatca aaaagttatg ggaagaattt gacaacaatc tagctgatcg tccagatcaa
     3421 gttacttttg agattcaacg ggaacatacg acaaatgctg cagcttggaa aaacggatat
     3481 attcgaatca ttaaaccagc taaagataca acaaatacgt gggaacgtgc agacattgac
     3541 aaattatctg caaatagcgg agaaagttat caagagatat tatcactacc tcaatacaat
     3601 aatcaaggtc aagcattcag ttaccaaaca atcaaagaat tacctgtacc aggatacgat
     3661 tctcaacaaa tagatgcaat gacatggaaa aatactaaac aattcacacc gttaaacttg
     3721 aaaataacga aaaattcctc tacaggtgaa aaggatctta ttggcgctgt tttcaaatta
     3781 acaggagatt ctattgatac tttactaaca gatcatggcg acggaaccta ttctcttcca
     3841 gaaaatgtca aattgcaaaa agaaatgacc tatacgctga cagaaacaaa agctccagaa
     3901 gggcatggat taagcaaaaa gactacttgg gaaatcaaga tcgcttctga tggtacggta
     3961 accattgatg gaaaaacagt cactacttcc gatgatacga tccagttgac tattgaaaat
     4021 ccttttgttg aagttcctgt agcagtacgt aagtatgcga tgcaagggac ggacaaagag
     4081 ataaatctta aaggagcagc attttcccta cagaaaaaag aagcaaatgg tacttatcag
     4141 ccaattgaca gccaaacaac gaatgaaaaa ggtcttgcca gttttgattc actcacacct
     4201 ggtaaatatc gagtcgttga aacagctggt cctgccggat atgatacttc gccgggaaat
     4261 tatgaattcc aaatcgataa atatggaaaa atcatttaca cgggaaaaaa taccgagatg
     4321 acaaataatg tatggacgct cactcatcaa aatcgactaa aagcgtttga tctaacggta
     4381 cacaaaaaag aagacaacgg acagacatta aaaggagcaa aattcagact gcagggacca
     4441 gaaatggact tagaatcgcc aaaagatgga caagaaacag atacctttct attcgaaaat
     4501 ttaaaacctg gaacttatac gctgaccgaa acttttacac cagaaggata ccaaggtcta
     4561 aaagagccag ttactatagt tatacacgaa gatgggtcaa ttcaagtgga tggacaagat
     4621 catgaatctg ttctgtcacc aggagccaaa aacaaccaga tttctttaga catcacgaat
     4681 caggcaaaag taccattacc tgaaacggga ggaattggcc gtttaggaat ctatctagta
     4741 gggatgattg gttgtgcgtt ttctatttgg tatctttttt tgaaaaaaga aagagggggc
     4801 agctaaatat gaaaaaactt ggttggctta gtatgtgtct cttcttgtta ctatttaaac
     4861 cagcttttac tcaggtagca acagaaacag aaacagaaat ggttcagatt actttacaca
     4921 aattgctttt cccaaacggg caactgccga aaaatcatcc aaatgacgga caagaaaaag
     4981 ctttattaca aacgtatcga ggattaaatg gtgtcacatt ccaagtttat gatgtcacag
     5041 attcttttta ccatctacgg gaaaagggca aaacggtaga agaagcacaa gcagagatcg
     5101 caaaaaacgg tgcgtcttcc ggtatgttta ccgcagaagc aacaactaca actcttaaca
     5161 acgaagatgg tatcgcttct ttttctctgg ccgctaaaga tcaagaaaaa agagataaag
     5221 cgtatctttt cattgaatcc aaagtaccag aagtcgtcaa agaaaaggca gagaatatgg
     5281 tagttgttct tcctgtacat ggacaaaaca atcaaaaact ttcaactatc catttgtatc
     5341 ctaaaaatga agaaaacgac taccctgatc caccttttga gaaggtatta gaagagccta
     5401 gaaatgattt tacgattggt gaaaaaatca cttattcctt gcatacgaca attcctgtaa
     5461 atatccttga ctatcaaaag ttcgaattgt cagatagtgc ggatgaagca ttaacgtttt
     5521 tacctaatag tttaacgatt tcatcgaatg gagaaaagct gacagaaggc tttgtcatac
     5581 acaagaaacc tcacggattt gatgttttat tttcgatccc ttcgttggaa aaatatgctg
     5641 gaaaaaaact gaccatttct tatcagatgc agctaagcag tacagcacag gcgaacaagg
     5701 aaatcaacaa caacggaaca ctggattttg gttttggtgt cagtacaaag aaagtctctg
     5761 tatatacagg gagtaagcaa tttgtcaaaa tcgagacaaa taaaccagat aaacgattag
     5821 ctggcgcagt attccttatt aaaaacaaag caggaaatta cctccagcaa acagccaacg
     5881 gatacaagtg gacaaagaac gaatcagatg cgcttcacct gatttccgat aaaaatggcg
     5941 ctttttcaat ttccgggttg aaaacaggaa gttatcgatt aaaagagatc gaagcacctt
     6001 ctggttatat tttaagtgaa acagaaattc cgtttaccat ttcaactttt ctttctgagg
     6061 ataaagaggc ggacagtata ttgaaagtag tcaataaaaa agaaaatagc cgtccatttc
     6121 ttccaaaaac aaacgaaacg aaaaatacac ttttaggcgt tgttggtatg gtattcgcaa
     6181 gctttgcaat ctggttgttt atcaaaaaaa gaacaggagt gaaaaaatga aaaatcataa
     6241 aaaaataaac gttatgttag gagtcctttt ccttatttta ccattactca caaacagctt
     6301 cggcgcaaaa aaagtgtttg cagaggagac agcagctcaa gtcatccttc ataaaaagaa
     6361 aatgactgat ttacccgatc ctttaatcca aaacagcggg aaagaaatga gcgaattcga
     6421 tcaataccaa ggattagccg atatttcatt ttcagtttat aacgtcactc aagaatttta
     6481 tgcgcaacga gataaaggag cgtccgtgga tgcagcaaaa caagcagtcc agtctttgac
     6541 tcctggtaca ccagttgctt caggaacgac agatgctgat ggaaatgtca ctttatcttt
     6601 acctaaaaaa caaaatggga aagatgcagt ctacacgatc aaagaagaac caaaagacgg
     6661 agtgtcagct gccgcaaaca tggttttagc tttccctgta tatgagatga tcaaacaagc
     6721 agatggctct tataaatacg ggacagaaga actagatact atccatctct accctaaaaa
     6781 tacagtcggt aatgatggaa cgttgaaagt tacaaaaatc ggtactgccg aaaacgaagc
     6841 actaaatgga gcagaattta ttatttctaa agaagaagga acaccaagcg tcaaaaaata
     6901 catccaaagt gtcacagatg gattgtacac ttggacaact gatcaaacca aagccaaaca
     6961 tttcattact ggtcattctt atgacatcgg caacaatgac tttgccgagg catctattga
     7021 aaaaggccag ttgatcgtta atcatttaga agttggaaaa tataatttag aagaagtaaa
     7081 agctcctgat aatgcggaaa tgattgaaaa gcaaacaatc acgccttttg agatcctggc
     7141 aaatagccaa acaccagtag aaaagaccat caaaaatgat acgtctaaag ttgataaaac
     7201 aacacctcaa ttgaatggaa aagatgtcgc aatcggtgaa aaaattcaat atgagatttc
     7261 tgtcaatatc ccattaggta tcgctgataa agaaggaacg caaaacaagt acacaacatt
     7321 caaacttatc gatactcatg acgctgcttt aacatttgat aatgattctt caggaacgta
     7381 tgcttatgcc ttatatgatg gaaataaaga aatcgaccca gtaaattatt ctgtcactga
     7441 gcaaacagac ggattcacgg tttcagttga tccgaattat attccttcat taactcctgg
     7501 cggtacattg aaattcgttt actatatgca tttgaacgaa aaagcagatc caaccaaagg
     7561 attttctaac caagcaaatg tcgataacgg gcatacaaat gatcaaacac caccgtcagt
     7621 cgatgtcgtt actgggggca aacgatttgt taaagtagat ggtgacgtta catcagacca
     7681 aacacttgct ggagcagaat tcgtcgttcg tgatcaagat agtgacacag cgaaatattt
     7741 atcgatcgac ccatccacaa aagccgtcag ctgggtatcg gcgaaagaat cagcaacggt
     7801 ttttacaacc acaagtaacg gtttaatcga tgtgacaggt ctaaaatatg gcacgtacta
     7861 tctggaagaa acgaaagcgc cagaaaaata tgttccatta acaaaccgtg tagcatttac
     7921 tatcgatgaa caatcttatg taacagcagg acagttgatt tctcctgaaa aaataccaaa
     7981 taaacacaaa ggtacacttc cttcaacagg cggtaaggga atctatgtgt atatcggtgc
     8041 aggagtagtc cttctactga ttgctggact gtactttgct agacgcaagc acagtcagat
     8101 ttagtctttt tgcaagaatt ggaaaaagga gcagcggatt acgcaaatgc tcctttttct
     8161 tttattatca aacagtcatc acaaggagaa cacaaatgaa accaaagaaa aggaaacttc
     8221 tagatctgct gatgcttttc ttgcttctct caggaatagg gatactagct tatccatttg
     8281 ttagcgacgc gctgaataat tatctggatc agcagatcat ctctcattat cagcaacaag
     8341 cggtcaaaga aaatgaagaa gtcatggcta aaatccaaga aaacatgaca aagaaaaacc
     8401 agcaattagc aaaagaaggt ggaaatccag gtgcagatcc ctttactaag aaaaaagaac
     8461 ctgcgaaaaa ggatagaacc tattttgaga aacatacgat cggtattttg acgatccctg
     8521 agatcaatgt ccatttgcca atttttgatg aaacgaatac tgtgctgcta gaaaaaggtg
     8581 cctcattatt ggaaggaacg tcttatccta cggggggaga aaatacacat gccgttattt
     8641 ccagccatcg aggactgcct caagcaaagt tgttcacaga cttaccgaaa ctgactttag
     8701 gtgattcttt ctatatcgaa atcaatggac ggacactcgc ttatcaagtc gatcagatca
     8761 agacggtaga accgactgat acaaaagatc tgagaatcga aaaagggcag gatttcgtga
     8821 cactcctaac ttgtacacct tatatggtca acagtcatcg cttacttgtc agagggcacc
     8881 gggttcctta tcatgcaaaa gaagttcaaa aagaagtaca gaaagtatcc gagaacaaac
     8941 gatttttcct gtatggattg ttagctgcat gtatactgat tttcctcctt cttatatatc
     9001 tttttcgtca atcgttcaaa ataagaaaca gaagttagct attcctcatt tctataatat
     9061 atctattcga atcgttccgt aaacaaacag gctgaggttt gaaaaagaaa acttcagcct
     9121 gtttttatga tgatatcaaa gagagcctat caaacactat ctttcttatt actaaatgag
     9181 gatcttttct taagttttcc gatattcttt aagaaggcct ttttgcatca attcttgttt
     9241 gtttttcaag ttgcggatat tctcttcaaa attttgagaa tatatcactg agaataacgt
     9301 atttaatacg taatcgaagg caatttgaga ggagaaagtc gctactttag caaaatcata
     9361 ttcctcctgc gatataacaa gacacatatc agcctgtttg gccatttctg aatgctgatt
     9421 tccggtaatc agcaaggtag gggcctctac atgcttcaaa taggtcatta ttcgttcata
     9481 atgatggatg cgaccgctgt agctaataaa aatggcgcaa tcctcgctag tcaaattggc
     9541 tgcattccaa gaagaatcac tgtattcttc agccatgatc aaaaacttat tcaacttgac
     9601 cattttattc tggaatttcc ttgccgtgat ttgagaatca ccttttgcaa aaagaaagat
     9661 tcgctgtgcc ttgttcaata ctttaccgat ttgatctaaa tcttcatgtt caatctgcaa
     9721 ttgtgctttt tttattgact ggatcgataa atccgccatt ttctttgcaa tttgctgagt
     9781 gctatcttca cttgaaaaag gaaaattagg atcaatcgtt tgattgaact gagcaatcaa
     9841 ttgatgaact tcccgcccca gctcgaaacg gaattcttta aatccgctga atcccatttt
     9901 tttgcataaa cgaatgatag ctgaatggga agtatacgta gcttttgcta attcttcaat
     9961 cgtcatgtag actgcagctt cgatattcct acggatgtaa tcgacaatcc gtttctctgt
    10021 cgtggtaaac aactcttgtt ttgttagttt ttcttcaata agcatgatct gacctccaaa
    10081 ttctatctta ctgataaaca cttggttccg ctactatctt tttgattttt atccttactt
    10141 tgaacattaa tttcaccaaa ccgaatgata acaagtgttt ttctgaactt ttttaccaga
    10201 atagctatta taaatacttt ctgcgctttt ctaggatata ctgaaaacat caataaaagg
    10261 aggcaccact catgcttaca atcgcttatc ttggcaatgg aaagagtaca aatcgctatc
    10321 atttgccttt ttcaagaaaa cttacgaaca aaatcaatat caaaaaaatt ttttccccat
    10381 ctgaaccagt gtggaaaaca ttcgaagaca tcgaatatac tgatcagctg tctgatcttt
    10441 gggatgatcc tgagatccaa ttagttgtca tcactacacc tagtcaattt cattatgaat
    10501 atggaaaaat ggcactcgaa catggtaaga atgtactagt agagaaacct tttgcagaaa
    10561 cgtcaaagga agcagaagag ttatttgcat tagcaaaaga aaaaggtcta tttatccaat
    10621 gttaccaaaa cagacggttc gattctgact tcttgacagt ccaaaaagta atcgaaagcg
    10681 gaaaattagg ggatatttta gaagtagaaa tgcattatga ttatttcaga ccggaagtac
    10741 cagaaaatac gcatgaattt tctactgcga acagctattt atatggacat gcttgtcata
    10801 cagtagatca agttctttct tatttcggcg aaccagatga tatcagctat gatgtacgtc
    10861 aacttttagg gaaaggacga atgaatgatt acttcgatct ggattttttc tatggaaata
    10921 tgaaaatttc gataaaatcc agttatttcc gcatcaaaga acgtccaagt ttcgtcgttt
    10981 acggaaaaaa agggatgttc gtgaaacaaa caaaagatcg acaagaagaa catctgaaaa
    11041 tgttttacat gcctgatcat gaagactttg gtatcgattt gcccgaacat tatggtgtat
    11101 tgacctatat agatgaacaa gggagttatc atgaagaaaa agtaatttcc gaggttggtg
    11161 attacagccg tgtctacgaa gggatttacg agacattagt taatggcgca gaaaaagtca
    11221 tcaaagatga agaaacaatc ttacaaatga aaattttgga agaaggaatc aaaagcattc
    11281 aagaatcaga aggaaaaata tgacatattt gtaagaatcg ggtttgtttt ttgacgcata
    11341 aactgtctta ttgtacgatg aaaaagtata gaactactag gaggaaccaa catgacaaat
    11401 tatgacggtg aaaaacacaa acgattaaga ggttcaatgg cccctaaaac agaagaagaa
    11461 gtggctttag caggaactaa ttctggtgaa acagaagaca aagaagaatc attagctggc
    11521 acaaactctg gtgaaacaga aaataaagaa acagctttat ctggttcaaa ttctgtagaa
    11581 gaggaaacaa agcctaatga gcccaatcac tttgaaaaag atatcgaaaa agcggttgaa
    11641 gatgtcaaag aaaaatttga agaactgacc tcaaaaaata aacatgatca tcattgatcg
    11701 gacgaaatca attcataaaa gcaggagaca tttggtcacc tgcttttttt ctgtatccat
    11761 tcgatagaaa tcgttcggta cagtcgtaaa aaaaatccaa ctgtaagttg taccaaacac
    11821 ccctcgattt aaaagcgcta ccatactaga atataagtag gaggtgaaca gcgctgtgaa
    11881 ctacaaaatt agttatctcc tgaaaacacc aagagaaatc agtctagaaa aagcagcaga
    11941 gatcatcgaa gtaaatacaa gcgaggcgat caaacaatta actccttttg ccgaatatgt
    12001 ggatgatacc cgtatccgta taaagaaatt ccgagcaatc gactgggcag aaaaattttt
    12061 ggatattact cgatttgaag ctgtgtatac cgaacaagag cgaagagatc ttctttattt
    12121 gatggtttat tcgactgcaa cgtctgtttc tgtttttcat tttcaagatt ttcttcaggt
    12181 ttctaaaggg acagtcctgt cagatatcaa aaagataaga aatgaattag aagtggaaga
    12241 ttgtcagctg tcgtatacta ggaaaactgg ttttaggctg ctagggtcag aagcaatatt
    12301 aagaagagaa gcaaaaaatc gcactgctcg tttgatgcag tcacttgctg gaagatttgg
    12361 gctcacgtat ttaaccgcaa ccctttccat tgattattac gccaaagtta gagatttgct
    12421 agataaaacg atttcactat cagatcttcg attcgcacca aatcgaatgg atgaattagc
    12481 ttattatttg agtttgacaa ccattcgaat tggaaaaaca tccatggaac tggtaaaaga
    12541 tgctgaactg ttggcttctc ttgcagtcac tgacattagt cgacaagtat tgactggcct
    12601 tggctattcc gaacatcaaa attcggagat tatatttttc tctctttgct tagcatcgat
    12661 cgttgaagga gatgtccagg agcctgcact tgattttttg ctggaatgtt cttcagaagt
    12721 catccatcgc atggaattcc tgatggccgt agaatttgct tcgtttcgtg aattattgat
    12781 gaatgtcttt tatcacttgg tccctgccta ttttcgtatt tcctattctt tttatttacc
    12841 taatgtgatg atcgatcaaa ttaagcatca atatgcttcc atctacgaaa tgacaagaaa
    12901 agcattactt ccgttagaaa gacgaatagg gaagagcatc cctgaagaag agattggttt
    12961 tttcactata ctatttggcg gagagatcag aaaagcggac gcagaagaaa gaaatagaaa
    13021 aataagagcc gtgatcgtct gtccaagtgg cattagctct tcattgatct taaaaagtga
    13081 acttcagcaa ttattcccga tgatcttttt tacagagact aattcagctg accgcttaca
    13141 cgaggttgac gaaacttctt acgatattat tttttctact gtacctctca cacttactga
    13201 taagaaaaag tctctctatg ttctgcatcc aatcatgact gacttggaaa aaaatcagct
    13261 gatcaatcaa gtacaaagag attggctgat accaggattt tcaatgcctt ctgcccaaga
    13321 attgttgtcg gcgctactgc cttatatcga attaaaagaa ggcgtagacg aagaaaagct
    13381 ctatcaagta ttaaatcgta aaatgaacaa acttttagaa aagaaggagg atcaacgtcc
    13441 tatgctatct gaattattaa cagaagaaat gatccaatta tcggatcaac caaaaaactg
    13501 gcaagaagca atcacatttg ccgcacagcc gctattatca gaaaataaaa tagaagaaag
    13561 ttatatcaaa gctatgatcg agcgcgtgga gcagtatggt gcgtttatcc atattggcga
    13621 tcacatcgca ttacctcatg ctcgtccaga agatggcgta aacgaagtag ggatgtcttt
    13681 gctgaaactt caacaacctg tggatctgct tgataatcca gcccatccca ttagtttgtt
    13741 catctgttta gctgctatcg ataatgaggc acatcttcgg gctttggcaa atttaacaaa
    13801 gctactttct aataaacaaa atcttcaaga tttattacac gcatcaacaa aagaagaaat
    13861 attagcgatc ataagaaaag gagacgaatg atatgaaaat ttttgcagta tgtcaaagtg
    13921 gattaggaac aagttttatg gtagagatga acatcaaaca agcacttgca gatcttggag
    13981 cagatgtgga cgagtttgaa gtaagtcata cggatgttgg aagtgtatct agcgatatgg
    14041 ctgattattt cttcattgag cattctttga aagatacgct aacttctata cctgatgaaa
    14101 agctcgtacc tttgcaatcg atcattgaca gcgaagaagt aaaagaaaaa gtaacgaaga
    14161 tcttgtcaaa agaataaaaa tgattcaagg gaggaactat caatggataa attgttgaat
    14221 ttgtttgtgt caatcgtcag tcagccctcc atcctcgttt ctttgattgc actgcttggt
    14281 ctcgttcttc agaagaagaa attttcagaa gtgatccaag gaactatcaa aacatttgtc
    14341 ggattcctag tactgatcgg cggtgctggt ttaatcagta attcactaac cccttttgct
    14401 tcgatgttcc aaacagctct gcatactcaa ggagtagttc ctagcaatga agcagtagtg
    14461 gcgattgctt tgcaacaata cggtacacct actgctttga tcatgctagt agggatgatc
    14521 gtgaatattt tacttgctag attcacaaga tataaattca tttttcttac aggtcaagca
    14581 atgctttatg tttcttgtct aacagcagtc attttagtaa gcgcggggtt tactgcaaat
    14641 ttgtggatga tcttattagg cggggtcttc gaaggaacac tattaactat tacgcctgct
    14701 ctttgtcaac cgtttatgaa gaacatcact ggaaacaatc aagttgcgtt agggcatacc
    14761 ggaaatatcg gttatgcttc tagtggatgg atcggaaagt tttttggaaa caaagaccgt
    14821 tctacagagg acataaagat tcctaaaagt ttcagctttt tgcgagattc tactgtatcg
    14881 atcatgctct tgatgtcgat cgtatatgtc gcacttgctt tgttagctgg tcctggattt
    14941 gttgaaagag aactaagcaa tggagacaac gctgtaatct ttgcactgat ccaagctgga
    15001 acattcacag ctgggtttgt tatcgtcctt caaggggttc gaatggtgtt ggcagaaatc
    15061 gttccagcat tccaagggat tgctaaaaaa ctcgtacccg attctaaacc agcattagat
    15121 gttcctatcg tttttccata tgcacctaat gctgtattaa ttggcttttt tgttagcttc
    15181 tttgtaggaa cactatccat gtttgctatg ataggcttgc aaactacagt tatcattcct
    15241 ggggtagtag gacatttctt ctgcggagct gcttctggtg tcttcggcaa ctcgactggc
    15301 ggtagaagag gggcggtatt aggttctgct ttcaatagct tattgattag ttggctgcca
    15361 ttatttatcc ttccagtatt aggagatctt aaaatcgctt cttctacatt tgccgatact
    15421 gattatctag taccaggact tgtacttggg aaactaggag aatacggtca aatagccttg
    15481 acagttggaa tcatcgcttt ctttttactt gtcgtattat ctagctttct ttttgccgga
    15541 aaaagaacaa acattgtttc taaagaacag aattaagcaa acttcaaaat aaaaattgct
    15601 tggacaatat cagaagaaga tttaaagtct tctgatatgt cgcaagcagt tttttgtttt
    15661 atgcatttat ttttgaatag cttgttaaga caagtggctg ttttgctaga agaggataac
    15721 ttttaccatt cattttcact gttccgcctg ttctatcaga agtaatcacg atcttatctt
    15781 ttgttttttc aagcgaaatc tttccatctt tgataggtag cgtacaattc agtgtttcaa
    15841 atgaatctag attaggttgt atcataaatt cttcataacc tggctttgtt ggatacaaac
    15901 cgataaaata gcgtcctaaa agatagattg ggcttgctcc ccatgcatgg cataagcttt
    15961 ttccataagg atctccatac atactgtact tttcatttcc tgtttgagta gggtcgtatt
    16021 cttcccagaa agttaccgca tcatgagata acatccctcc ccaataatca agaatcgttt
    16081 ggtagacgat attcgtctgt ccgagtttgc ataaagcatc ttgttcatag aatttgaaat
    16141 atggagtagt aatctgtgtg atagtgtcat tcaataagac attctttaag atcgattgct
    16201 gttggtcttt gttagcaaaa tcgaataaaa cagcgaaaat attcgcatga cgcgtcacat
    16261 ttcttctccc agattcatag caatcgataa aagcgccaag ttcttcatcc caaaagtagt
    16321 caaataggtt ttgtttcaat agttgatatt tttcttgata gagatgagta tgataatttt
    16381 cgttctctcc atatgtttca gctaagatat ctccgcaagt gatcatggaa tgataggctt
    16441 tcagtaataa gacttgttct gcagcaagcg ttccttcttt atccatttca gaccaatcaa
    16501 taaatatcca atcttttgct ctcccataaa taaaccctaa ttcattcgtt tgctccatca
    16561 agtatctcat taatgattgc atcttagggt agacgatgcg taaaaattca tagtctttcg
    16621 tcatcatata gtggttttcg ataccaatca accacaagat cgaatagtca acgatagtat
    16681 tcatatgctg cttgatttca ttttgtcctc ttaatgctaa tatcgttcgt ttattgattt
    16741 cctcgtcaaa aaaaagatat tgattgatca tgaatgattg atacgcatct ccagaccaaa
    16801 tccagcgatc tcgtttgata ccgtcgataa aaaacaaatc actacataac ttgaaggctt
    16861 cttctgatac tgaccagatc tgattcagta aagaagaatc gcttgaaaaa gcagcttgtt
    16921 tcgaaagaga taaaaattca tgaaaaactt gaagttgaac cattccagta gaaacctctg
    16981 gtataaacaa atagcgaaaa gcgcgtttac gagctttcat ttttcttgtc attcctattt
    17041 gcttatagta gcaataatcg atatccatag cttctacatc ggactctcca taacatagag
    17101 tgatagattc gatatggtca tccagagata ttagttctac acagccattc actgctcgcc
    17161 caaaatcgaa taagattcct ccgccaatct ccgtttcagt gactggatgg acgcattctt
    17221 tttgataata gatttgattt ggatcttttg cgatatcgac ataaagcgga ctccagccag
    17281 ctggatattc ttcaataaaa ttacttgctg tccagccaat atctgatttt acttcgtctc
    17341 cctcgataaa catcgctggt agccctgatg tatgtcctac aaagatacga atttttgctt
    17401 taccagctgg acaagtcagc cattttccac atggatgttt ttgtccatta atttctacat
    17461 acccaactcc ttggatcgtc actttaaaca tggaaggttg atcaagaaag taatatctct
    17521 taaacttgac gttccgatgg caatcatcca tgtaccagta cgctggccag tcaaaacctc
    17581 gttcttcccg ttggaaattt tgtagcagtc catggcggat ctcaaagtct ccaggatacc
    17641 aaagccatct atctttatgt ttttcatttg tcattttaca cgacctccat caaatttatt
    17701 ttgttgtcaa ttgttctttt atttcttcat ctactcttat cactgtcttt ctattcgtcc
    17761 tgtgtactac tagaaggcta ataagcgcgc taatcaacag caagatactg ccattgacga
    17821 aaacagcaag tgttttactc ccaagagaag caaaagagag aaagagcaaa ccatatggtg
    17881 caatgaaagt acctaaatta gcgccaacca ataagattga agtattgaaa ctctccgcgt
    17941 acttgggcgc ttggatactt acttggttga ataagtaagg aatgaataac gaaaaagaaa
    18001 ttcctgttag gatcgcacca aacccaatca catacaggtt tatcacagtg gcaatcaata
    18061 caaagccgat tcccatcagt agtaaagaaa gtgtaagcat atagcgatgt gcttttgtct
    18121 gaagccagcc aaaacaaact ccggcagcca tacttgccaa ttgcatgatt gttaagatat
    18181 tcgctgcatt agtttcgttt cctagagaat aagtcgtcag gactgtggct aatttaatcg
    18241 atacagcatt atataccacg atgaccagca agagaaaaat accataacta atcattttag
    18301 gattcgtttt gattacttgt cttgttgggg ctgtgccatg cgattgaagt tcagttgttt
    18361 caggtgtagg aacaaacaaa acaaacaaga caagaatagg taagctgatg gcgtagacta
    18421 aaaaggtata ttgccaaccg aactgtacta gctgtcctgc aacaaatgtc attaaagctg
    18481 caccaaatcc ttgaaatgca ctttgaaaac caatcataga ggctcgttca tctccgtgat
    18541 aaaaggcagc aatcatgctg actgccaaag aattgatcaa tccaaagcca atacctagtc
    18601 cagctcttga agctaagaca atactgtaac tttgtgagaa gagggggagg ataccacaaa
    18661 tcgatgcaat cactaatccg atgatcacag tttgtttttg tccgattttc ctagcaataa
    18721 aactactaat caaaacaaaa atagtgacca taaaagaggg agcagtcgtt aataattcaa
    18781 cagcagataa agaacgcgtg gcataagtag ctgacatact gggaattgca gacgtgactg
    18841 caccagcaga agtcaaaata agagagactg acagcagaga aaatttcatc atctttttat
    18901 tcataagcat gttcctttca tttttgtaag cgcttatcaa aaatatagta tgaagtggtt
    18961 attctctcaa tagcggaatc acaggaagtt catgtcccaa ccatagaaag tctttatgaa
    19021 accaaaaata gctttgcata taatagcagt acagcgtgaa ctaataagcg aaagaaaccg
    19081 cttacaaaaa ggagggagtc aattggaact agctgaatta gacatttttt ttgcgtaacg
    19141 aagaatcaat cgaaactctg cagcgtgaac aaggaatgaa tatcaatgat ttaaacttag
    19201 cttaccatct tcagacaccg ccaaaaatcc accgagggga tctatttcaa cagggaaata
    19261 ttcatatcag taaacaccgt cgatatgcgg aagttcccga gcatacacat gattttgttg
    19321 aaatcaatta tatgtatagc ggtacatgtt taaaaattat taatggagaa aggatagaga
    19381 tggaagaaag tgacgtggtg atcattgata aagaagctac tcaaagaatc gaatacacag
    19441 ggcaagagga tattttaatc aacatcttat taaaagaaga aaccttatct acagagatat
    19501 tgaatcattt ggcaagcaca aataatctac tttcttcttt tttgataaat gcatcaaaag
    19561 aaaaagggga acatacacaa ttcattcatt ttcgcggcgg aaaaacaagt cgtttgacgc
    19621 atttgattga aaatatgctg ttgatttatt ttgatacata cagtcataag atccgttcgt
    19681 taaatctcta tacatcacta ttactgatcg aattgactca tttattagaa caacaaagct
    19741 tttcatcagc agatggatgc gcagaaaatg agatgcttag agtattagat tatatcgata
    19801 agcattttac ttcttgtacg ttaaaagaaa cagcagctca ttttggctac aatccggtct
    19861 atcttagtaa taaattgaaa atgtattttg atgcttcctt tcaagaactg gttctaaaga
    19921 aaaaattcga ctttgcgttg gaactgattc aagaaacgag tttttcgttg gaagaaatcg
    19981 ctaatcgtat cggatataaa caaacgactt ctttattcaa attattcaag aagtattcaa
    20041 catgtactcc caatgaatgg agaaataaac attaagaaga aaatctttca ctaaagcata
    20101 tgaaggaaaa ttgttttagg tgcgattgct gtcttattat atcgttgcag cgaatttttt
    20161 tggatttatt ttcagttcta aacttttggt tgtattatca ggggatatag gtgtaaacat
    20221 ttgtcattta tctgttatgc gtattcagga attcgtttcc atcaaaaatt ataataacaa
    20281 ctattataat taacatgttg ataaaatgaa taaacaagac tatacttttc atgaaagtaa
    20341 caaatagtta tgaaaaggag ctgtttattc atgaaaaaaa caagtcgtaa agtagtaatc
    20401 gtcggtacag gttttgttgg aacaagtatc gcttatgcaa tgatcaacca aggaatctct
    20461 aatgaattag tattgatcga tgtgaatcaa gaaaaagcag aaggggaagc attggacctt
    20521 ttagatggta tggcttgggg cgatgaaaat gtggctgtat ggtcaggtgg ctatgaagaa
    20581 tgtaaagatg ctgatatcgt tgttatcaca gctggtatca accaaaaacc tggtcaatct
    20641 cgtttagatt tagttaaaac aaatgcgtca atcatgcgcc aaatcgtaaa agaaatcatg
    20701 ggatcaggat tcgacggtat tatcgttgtt gcttcaaacc cagtagatat tttgacatat
    20761 atcgcttgga acgaatctgg tctgccaaca tcacgtgtta tcggtactgg aacaacattg
    20821 gatacaactc gtttccgtaa agaaatcgca ttgaaattaa aagttgaccc acgcagcgtc
    20881 cacggctata tcttaggtga acatggtgat tcagaagttg ctgcatggtc acatacaact
    20941 gtcggtggta aaccagtgtt tgaaattgta gaaaaagacc atcgtattgc ccaagatgaa
    21001 ttagatgtga ttgctgataa agtccgtaat gctgcttatg agatcattga tcgtaaaaaa
    21061 gcaacttact atggtattgg tatgagtact gcacgtatcg taaaagctat cttgaataac
    21121 gaacaagctg tattgcctgt atctgcttat ttaactggtg aatacgacga aaaagatatc
    21181 tttacaggtg tgccatcaat cgttgacgaa aatggtgttc gtgaagttgt tgaattatca
    21241 atcaacgaag aagaaaaagc aatgttcagc aaatcaacaa gtgctttaag agaagtattg
    21301 aacactgtat tataagaaac aatgaatatg aactaaaaat cctaaaactg cttaacagct
    21361 gaaatagctg ttaagcagtt ttttattgat atctaatagg taagcaaata aatagaatgg
    21421 ttaaaattaa tcggtccgaa aatgtatccg tacatatgaa catatgaaat ttaatctgtt
    21481 gattacaata agcgaatcgt tatcaatgtt tatctgtggt taattaaaaa atccttccgc
    21541 aacggcaaat acaatgattc atatctaatt cgttcatatt tgattaact
//
LOCUS       DAZAFF010000044        21142 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000044 DAZAFF010000000
VERSION     DAZAFF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.044"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..1528)
                     /locus_tag="ACG9BB_001564"
     CDS             complement(137..1528)
                     /locus_tag="ACG9BB_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFN2837508.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1741..2127)
                     /locus_tag="ACG9BB_001565"
     CDS             complement(1741..2127)
                     /locus_tag="ACG9BB_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFN2837509.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2183..2806)
                     /locus_tag="ACG9BB_001566"
     CDS             complement(2183..2806)
                     /locus_tag="ACG9BB_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFN2837510.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2870..3538)
                     /locus_tag="ACG9BB_001567"
     CDS             complement(2870..3538)
                     /locus_tag="ACG9BB_001567"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFN2837511.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3550..3990)
                     /locus_tag="ACG9BB_001568"
     CDS             complement(3550..3990)
                     /locus_tag="ACG9BB_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837512.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4049..4720)
                     /locus_tag="ACG9BB_001569"
     CDS             complement(4049..4720)
                     /locus_tag="ACG9BB_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837513.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4905..5303)
                     /locus_tag="ACG9BB_001570"
     CDS             complement(4905..5303)
                     /locus_tag="ACG9BB_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFN2837514.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5300..6073)
                     /locus_tag="ACG9BB_001571"
     CDS             complement(5300..6073)
                     /locus_tag="ACG9BB_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFN2837515.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6070..6753)
                     /locus_tag="ACG9BB_001572"
     CDS             complement(6070..6753)
                     /locus_tag="ACG9BB_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFN2837516.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6920..7807)
                     /locus_tag="ACG9BB_001573"
     CDS             complement(6920..7807)
                     /locus_tag="ACG9BB_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2837517.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7804..8673)
                     /locus_tag="ACG9BB_001574"
     CDS             complement(7804..8673)
                     /locus_tag="ACG9BB_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2837518.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8810..10096)
                     /locus_tag="ACG9BB_001575"
     CDS             complement(8810..10096)
                     /locus_tag="ACG9BB_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2837519.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10369..10947
                     /locus_tag="ACG9BB_001576"
     CDS             10369..10947
                     /locus_tag="ACG9BB_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN2837520.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10947..11876
                     /locus_tag="ACG9BB_001577"
     CDS             10947..11876
                     /locus_tag="ACG9BB_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2837521.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11873..13609
                     /locus_tag="ACG9BB_001578"
     CDS             11873..13609
                     /locus_tag="ACG9BB_001578"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN2837522.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13672..14508)
                     /gene="pheA"
                     /locus_tag="ACG9BB_001579"
     CDS             complement(13672..14508)
                     /gene="pheA"
                     /locus_tag="ACG9BB_001579"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFN2837523.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14505..15014)
                     /locus_tag="ACG9BB_001580"
     CDS             complement(14505..15014)
                     /locus_tag="ACG9BB_001580"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFN2837524.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15007..16302)
                     /gene="aroA"
                     /locus_tag="ACG9BB_001581"
     CDS             complement(15007..16302)
                     /gene="aroA"
                     /locus_tag="ACG9BB_001581"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFN2837525.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16315..17406)
                     /locus_tag="ACG9BB_001582"
     CDS             complement(16315..17406)
                     /locus_tag="ACG9BB_001582"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFN2837526.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17423..18589)
                     /gene="aroC"
                     /locus_tag="ACG9BB_001583"
     CDS             complement(17423..18589)
                     /gene="aroC"
                     /locus_tag="ACG9BB_001583"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFN2837527.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18591..19655)
                     /gene="aroB"
                     /locus_tag="ACG9BB_001584"
     CDS             complement(18591..19655)
                     /gene="aroB"
                     /locus_tag="ACG9BB_001584"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFN2837528.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19688..20710)
                     /gene="aroF"
                     /locus_tag="ACG9BB_001585"
     CDS             complement(19688..20710)
                     /gene="aroF"
                     /locus_tag="ACG9BB_001585"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFN2837529.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         6107 a         4633 c         3694 g         6708 t
ORIGIN      
        1 caacctctta ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa
       61 aaatttgaga aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat
      121 cacaacctct ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat
      181 cgcgatctaa atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga
      241 cgtttactgc tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga
      301 ttgctactgg caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac
      361 cgactccagc tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt
      421 ccaaactaaa gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat
      481 aaacgccagc acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg
      541 ttgcatcatc tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag
      601 cacttgaacg tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga
      661 ttggattgac ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg
      721 ctacgtaaga agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg
      781 ccaaaacgtt tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca
      841 cgatccgcat aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt
      901 ttggcgcttt tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga
      961 ctccaatcgt acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa
     1021 aggataaaat ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt
     1081 cagagattcg tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca
     1141 gcattgttga accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca
     1201 atacagtcgc actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa
     1261 tgatcgaaat aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac
     1321 ctacaatccc gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca
     1381 attgaagcag accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa
     1441 attttgtatg gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa
     1501 ccactaaaac tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc
     1561 catcgaagga agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaatttt
     1621 tttcagtgac ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa
     1681 ttagtaataa aaaaacatac ctgtgatagc tttaatctat cgcaggtatg tttttactgt
     1741 ctattcttgt ttttctgatt gaaaattcaa ttttacccca ggctgatcta aggcgatctc
     1801 tgtcacctca taagacattc ggcgtacgat atcgaataac ggtgctacta gctcgtccac
     1861 ttcttcttgt gtgacatcat tttgaccttc aattttccga ttgaggatat gattgatctg
     1921 actcacactt ccagataaaa catatgtatc aaacactaaa cggaactcta agcgtgctcc
     1981 tacgatgcta tgttctttcg gatagttttc gatcgtttct tttaaaggcg agaagcctac
     2041 tttcaaatct gtttctactt cttggttcgg caatctcata tcataatgaa atgcttctac
     2101 aacttctttt tgtcttctga tttccatata attttatcac tcctttatga aaattataac
     2161 atatctcctt tgaaagggga tactactttt taatgatgct ctgcaaggta aggatttcat
     2221 tcagatttcc taccacataa tctgccggga tctcacccag aaaatgatcg atgtcgtaaa
     2281 gacaagtagc gactcctgca tttttccctg cttcgatatc taaacgacga tcgccgatca
     2341 tcatcgtgtc tgttctttct aaatgaaaag tatcgatcaa ataatttaaa gatgctggat
     2401 caggtttacg aggaaagtcc tgatcgatcc ctactacttc ctcaatcaga tgtgccaatc
     2461 gatgttcttt taacaggccc catgttgatt ctgtcaaacg gtgggtcagg atgaaatgac
     2521 gaccgccgtt gtcttttagc atttccagta cttgtttcgt ttctggaaat ggacgggaaa
     2581 cttccgtact ttctttttca tatcggtgaa ataattcatt aaactctctg gcattcaagt
     2641 ttgattcatt gatcaaataa ctagtagaat actttttcat tttgaagtag atttcttttt
     2701 tgtccatact gataccaaaa tcttttagtg cttgccatgc accatctacc attgcgggat
     2761 acgtatcaaa caatgtacca tcaaagtccc atatataatt tttcatgttc cttttgttcc
     2821 attcctttgc ttcttttttc tatctctcac ttttagataa aggtgttctt taaagtattt
     2881 ttttgtccag aattgtcaaa tcatctgcta atgtatactg gattggttgt gcatttggga
     2941 tatcgatcgt atccatttga tcttcaggaa catcttctaa atattttgtc agtgctcgga
     3001 tgctattacc atgacccaca actagtaaat cttgccgatc ttttagtttt ggtgctagat
     3061 gatcttgcca taagggaatg atccgattca ctgtcatttc cagactttct ccgtatggga
     3121 tcaaatgcgg atccaagtga tcatatctgc ggtcaaatct attttctgcg gttaacggcg
     3181 gaacttccgt atatcctctg cgccatgttt ttacttgatc ttttccgtat atttctttca
     3241 tcttatcttt gtttttaccc acaagatccc cataatgccg ttcatttaga cgccatgttt
     3301 ttattatagg aagccataac tgacccattt cttctagtgc gacttgtccg gtaagaatcg
     3361 cacgtttcaa aacagaggta taaacggcat cgaattgaag tccggcttca agcatttttt
     3421 gaccggcaac tgctgcttgt tcttttcctt tttctgttaa ggcgacatct agccatcctg
     3481 tccagtaatt ttcaaaattt gcttcgcttt cgccatgacg taataatact aatttcattc
     3541 ttttctccct taaacattcg tttcgaattt tataccacat gttttgcatg tcacacgtat
     3601 tcgtcctttt cctttaggtg cacgctgctt ttgatgacaa ttcgggcact caaaaaaagt
     3661 ataacttttt cgatccattt tgacttgctt tttgtttttt atctgatgat atttttcttt
     3721 gactctatca agatatttca aataacgttg attctcattt agtctggggt agattttttt
     3781 tgataggaaa cgaaatatca tccatccgaa aaagacgatg aacaacaatt gtccgattct
     3841 atagggcaac caacgactta gcaaactaaa gacaagcgca gcgatcaata gaccgcgatt
     3901 caactgatca aatttcgcat atcttccctg cataagtttg tgatagttct gctgaaatcg
     3961 tattagcctt tctaaccatg tttgcatcat attatctcct ccatcatcta atatttatta
     4021 tgacaaaaaa atccaaaaag aaaaagcctt aattggcctt ttccattgca acttggtgaa
     4081 ggacattccg atttctggta aagtcaatca caatcacttt ctttttatct tctctgttga
     4141 ctactcgaaa tgtgtccgtc aatggaagaa acagcttctt agatggtagc tgttgcatac
     4201 gtataaaacg atggatcatc gtaatcaatt gaggaaattg aatagacgtg tgaagcagac
     4261 ctggatattt tttcaacagc tgtgtcgttt tgatcagatt ccgtttttta ctgatcactt
     4321 ttaaaaagct ttttagaatc ttttgctgac gtctaaaaat agaaaaagaa ttttcgtcat
     4381 ctatttgata acgcatttgt ttttgcagtt ctgctgcagt cagatggatc ttcccatcgt
     4441 ttgtatcgat cattaaacct tctggaaaca gttcaaccac tgctcgatcc caagattcaa
     4501 gagaaagctc gatataatct tttactggta attccaattc taccttaaaa aagtgcaaaa
     4561 cagccgacag cccttctgct tgatagattt ctcctaatgt tagcccctct agatggaact
     4621 ctggtgaaaa atgcagcaat gttttatttt cctgcaattg aacatataga aatgtaaatc
     4681 gctcaatcag agtatctttt aaaacaacta aaaaattcat ttttctcccc cttttttcat
     4741 catttttaaa atagcatatt ttgaaaagaa agtctgatgc ttagagtaga atttcataaa
     4801 aatttactat ttcataagta ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct
     4861 catccgcctt cttgtttgtg tttatttcct cttcaaacgc ttgtctatga ttcagacttt
     4921 tctctatcgt tattttcatc taaaagttct tgcattccga taatttctcg atattgatga
     4981 atcttagtga taccaatgac tttgtctaca tcaatactaa aacaaatacc attacctgga
     5041 atatcgatgt tgatgccttg accaatcgca tccattactt tattcgtcaa actatcgggt
     5101 actaatgtca acacaatatc tttttctggc gaaatgtcca aacctaaaaa tttccctgaa
     5161 tcatgtacac cagaacctct tccatgtaag atcgttccgc ctgctgcacc tgcttctttt
     5221 gaaaagtcga taacatcttc gcccattcct ccgtcaacga tcgtcacaat catgtgaaga
     5281 ttcaatgcta caagatcttt catttgcttt cccccaattt ttgcaatctt tctttttcga
     5341 ttctgtcttt ttcatctttt cgtttataaa tatatcctag tactaaagtg gataatattg
     5401 gtgtcaatgc aatcattgca accacaccaa acccgtcaat gatcggatct cttccttcaa
     5461 tttgtgcagc gattgccacg ctcattgata aaataaatgt cgaacacatc gggcccgttg
     5521 ctactccacc gttatcaaaa gccattgcta aaaagatctt gtccactttt cgtcctaaaa
     5581 taaatgctag cccgtatcct gggatcaaga aataataaag agagaatccg acgagcaaac
     5641 gccacataga caatcctact gctgcgccga tcccaattga aattaccatc agcatgattt
     5701 tcgatcggat tctcccctcg ctcaattctt caacctgttt gatcattaca tgaatcgccg
     5761 gctcagcaaa ccctactaag aaccccatta aaaagccaag gggaaccagt atataattgt
     5821 aaggcaatcc cgcaatagca ctgcctaaat gctgtcctac cggtacataa gccatattga
     5881 ctccgtgcag gaaaaggatc aacccaatag tcgttatcaa gaaacctttc ataatgtcaa
     5941 agaaacgctt tttgcgcata cgaaaactaa tgatattcat taaaacaaat aggattacga
     6001 taggtgccac tgccaaaatg acttccatgg taacgatttc gattccatct atcacttgct
     6061 gccagaaact cattggaaga tcacccccaa aatcatcacg gcaagaatcg gccctaacga
     6121 agcaataccg accattccaa aactgtcatt ttcatttttc ttttgtttga tcatactagt
     6181 catccctccg gcaagggcca agataaatgg aacagttact ggaccagtag tcactccccc
     6241 cgaatcaaaa gcaatcggca taaattcgga actggtgaaa aatgaggcga tcaaaacgcc
     6301 tatgtagcct atagccatca attgataata ggatagcttg aatacgaccc gaagcagcgc
     6361 aaaggctaga aatactccag tgccgacgct gacgatccca atcagcaaca ctctgccaat
     6421 ctctccgtca gatatatcat atacttgttg tcccaaaact tgcacggaag gttcagcaat
     6481 cgtgatcacg atcccgatag caaatcccaa actgaccaaa acggtcaaac ttttcttttt
     6541 gatcatatat ttccctacta gatctccgac ttccatcata gaataatctg ctccaaacaa
     6601 aaagagcgtc attccgatca tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc
     6661 tccttctaac ggtgcaaaaa caaacgttaa aatgacaatc aggacagtca tcggcaatat
     6721 agcagcaatc acttctttaa aattttttag catgtatcct cctcctttac tctttaagtt
     6781 taccttaaaa tgaaagagtt ttcgagcaca aacttaaaac gttatcattt aatttttctc
     6841 aacataaaat aacacacgtc ccaaaagtat ctttttactt ggaaaaatga ctttgaagga
     6901 acgcgcttat tgtgtttttt tattttacag agccgatcat tccttgaacg aattgctttt
     6961 gcaaaacaaa aaatacgatg gcagttggta aagtagcgat gactaatcca gccatgatca
     7021 ttccatagtc tggtgtatag gaagcaccca tagcagataa aacaagcgga accgtccgtt
     7081 tatcaggaga ttgcaaagct actaagggcc ataaataatt gttccagcta ctcatgaatg
     7141 tgatgattgc agccgcggca taggtatttt tagccgtcgg catatacact ctaaaaaaaa
     7201 tacttagttc ttttagaccg tctaatcgag cggcttctac tagatctttt ggaaaagatt
     7261 taacattttg tcgaaagaag aagatcaaaa aagcagtgct aacagatggc aggatgacaa
     7321 ccaagaaact gttgattccc aaaaccgttc cgttcaattg tgaaaacatt cgatataaag
     7381 ggatcatcaa tgccgcaaaa ggaaccatca tcgatagcag tagaatattg aaaattaggt
     7441 cttttcgttt actttgataa atctcaaatc catatcctgc cattgacgaa atcagcaatg
     7501 caaacacagt ggtcaccaaa gcgatcatcg ctgaattcca aagggaacga gtaaaattca
     7561 gctcattgga aaacaaattt tgaaaattga tgatcaagtt actgccaatc tttatttttc
     7621 ccgatgtgat atccacgggt gtattcgtca ttcccaaaac catccagaca aaagggaaaa
     7681 tggaaataaa tgataggatg cttaatgctg tatactttaa tactgaccca attgtcgaac
     7741 gaagttctaa tgcttgtttt ttgacgttgg ggcgttcgat tgtttctttt tctaccatat
     7801 ttttcatcga ctttctcctc ctactttcgt ttggattatc gacaatatca caataaagga
     7861 aacaataaca aaggaaactg ctgccgcata tccaaaatta ggcgtgaatt tataactcaa
     7921 attatagatg tattgagata gggtggtcgt tgcatttgct ggtcccccgc cagtaatatt
     7981 ttgtacttca tcaaaaagct gcaatgtccc gatcgtcgaa gtaatggaag taaacaaaat
     8041 gattggtttt aaatttggga tcgtcacttt gataaactgc tgccatttag aagcaccgtc
     8101 aatagatgct gcttcataaa tctccgaatc aatattttgc atgcctgaaa ggaaaaaaat
     8161 catattgtat ccagtccaac gccaagtgat cgaaagaatg atcagaactc tcgcccaaat
     8221 tggatgagtc aaccagccaa tcggtgtgtc aatcatacca atgcccaaca acaagttgtt
     8281 tacaaggcca gattgagaaa atagactttt catgatcaga gaataactga ccatcgaagt
     8341 gatacatgga agaaagatag cggttcgaaa caacccttta tatttcaact ttttatcatt
     8401 taaaaggttg gaaataaaaa gtgctagaaa caacatgatc ggtacttgta caagtaaata
     8461 caggaatgta ttaaaaaaag cttttttaaa agttgcatct gtcaacatcc gctgataatt
     8521 acctaacccg ttaaatgtca tattgacagg gggaccactt tgaaaagacg tgatcaaagc
     8581 caaaaccatc ggaataaaaa ccatgattga tatcaaaatg actggcaaga ttaaaaaagc
     8641 atgcccgttt ttgaataaat ctttctgctt cattcttttc cctccaattc tatcattgat
     8701 tatctacatg aaaagacaag aaagacaata tctgtaaggc agacgtgatc ttttttgtct
     8761 tttcgtttgt ctgatgaaaa agcgaagaaa ttcacttttt taccaccgat caattagcca
     8821 attgtgcttc tacttgtttt tgtgtatctg ctagtacctt gtctgtctct tctccattga
     8881 tgattcgatg aagagcttca gctacgactg cttcaattgc ataggtctca tgtccgtaat
     8941 tcacttctgg tatttcagat gtccatgttg aaaaatcgtc aaagatcttt tgaccactgt
     9001 aaaattcaga agcttcttga taagcttctt gttcattcgc gctaagcatc gtagatacta
     9061 gtccgatttc tttcgcaaga gtgcccatca gttcttcatt cgaagcaaac gttgcttcta
     9121 ggaaatcttt cgctgaatct tctccagcta ctcctttgat cacataccag ccagcgccgc
     9181 caaggttaga agcttgcgct ttttgcagat tttctggaag tgcaggtatt ggtgcaatct
     9241 tccattttcc agattggttt tctgctcctt ggatcgttga agaataccaa gcgccagttg
     9301 ggctagaagc tactgctcct gattgtatcg cagttactcc agcattccag tcagaggttt
     9361 gttcgactag atcttctttc aaaagagtgg caaacaattc aagaccatac tttaaagatg
     9421 cattgtcttg gatcgtgact gtctccccat cttcagatgt ataccattct cctgcctgtt
     9481 gcataatcat ccttactcga ccaaggtcag aaggattgac ttcagcgatt ttctttcccg
     9541 tcttttcttt gacatctcgc gctacttgaa tatactcgtc ccacttcaga ttctccatat
     9601 cctcttctgt ataacctgct tctgccatca gatccgtacg atagaagttt gctgttacgc
     9661 ccgaatcgaa aggaacaccg tatattttat ctcctacttt gtttaccgca aatttatacg
     9721 gtgcaaaatg atcttccttt acaatagcag acaaattagc gaatgcatct ggataagaag
     9781 tcaaatagcc ttgtatccta taatcttcaa tcaagacgat attgggcaat ccttctgtat
     9841 tcccgctcgc taaagcggtg ttcagtttct gaacgatatc atcctgagac atcgtaacaa
     9901 cttctatctc tgtttcttcg ttctcataaa ctttttttgc ttcattcact gctttgatat
     9961 tgaatgtctc atcccacgcc cagacagtga ctttttcttc tttgtctgct gtactactag
    10021 cacttccgca tcctgttaag aaacctgatg ctgccgctaa acaaacgact ccagcaatta
    10081 actttctgat gttcattttt atccgctcct tttccatcca ttagttgttt acgtttgcta
    10141 cattcatcat accaaaattt gaaaacggtt tctttattat tcagatactt caaaaatttc
    10201 tcctaaaata ataaaaaaat acaaatcaac tatgacaaaa ggaataatct tagcatctag
    10261 accaaagatc gaaatggtga ggtttcttta aaataaaaat atccttgcat tttctcctag
    10321 aaataagtat ttttaaatta caagactatt cttaaggcgg gtaaaaaaat gccaatacgc
    10381 gtaattctta tcgatgatga acagacgatt ttagatggaa tgaaacagtt gatcaattgg
    10441 tctgagcacg ggtttcaatt aatagggact ttttccagtc cgctgaaagc gcttgatttt
    10501 tctttggtac atgcaatcga tatcgtggtc acagatgtaa tgatgcctga attaaatgga
    10561 atcgaattat cgcggctatt aaaacagcaa caacctgaaa cacagatcct tatcctctct
    10621 agctatgatg aatttgatat ggttaaagat tcttttaaag aaggcgtcgc tgattatctt
    10681 ttaaagcctc gattgagtcc tgattttttt ctaaattcct taactgccgt ttcacaaaaa
    10741 atcaaaaaaa agcccaacaa aagcccaatc accaatcgtt atgagcagat ctgcgagaat
    10801 ctttcacagc agattgctgg acaaactcgt ccattgcttg accctcttct ttttgaacac
    10861 accttgtttt tctgttatat accgaaaaac ggccattcag ttcaaaacaa ggctctaaac
    10921 aaaaacagtc gatcacccat tcattgatgg atagcgacga ttcgtttact atttatcctg
    10981 tttctacctt ttcggaagaa tccggctatg taatcaatac tgattccaaa gatacactta
    11041 ggaagtttgt tcaaaagttt cgtcaagaag tttttccagg tgattttttt gttgtttctg
    11101 attcttttgc aatccaagat atcaatgaaa tattccaaga gatcagaaat tgcaccaaag
    11161 gacaacgttt ctatcataag ggaaagaacc ttgcttatca gcatgaactt cttccgttat
    11221 taaccaacca cgagcagcaa gcaagtagtt atttaacaaa aatcgttcat aaagactacg
    11281 ctttagctgt caaagaactt gagaatttct tcacgacgat attggaacag cctattgatc
    11341 cagcttattt gaaacatgag gcaatcaatc gtttctatgc attactcaac gctttggctg
    11401 acgagcatgt tgggaatgaa gaactttctt tgctcaagat cgcccttcct tctttattag
    11461 ccgatgctga gtatttcgaa gatttttcta ttcttgtcaa agaaacactt gagaaactca
    11521 cccacttttc tgcaaatgca aaaaaagaca atttcgatat cttgactttg atctacgagt
    11581 tcgtccagga aaattatgag aaagaaatca atttacaact tttatccgaa aaatatcatt
    11641 tttcatatag ctatctctca gccatcttta ctgaaaaatt tggtatcaac ttttcaaaat
    11701 atctaaaaaa agtccgtatc agtaaagcca agctcttttt gacggaaaca cggcttactt
    11761 tgacggaaat ctgtgagaaa gtcggctata ccgaactggg ttatttctcc agagttttta
    11821 aagaagagac aggaatgaca ccttctcaat accgtaaagg aacttttgta aaatgagaaa
    11881 aaaaatcaaa gagcatgaac tctttgccaa acttctgttc attttgttca tcggtctttt
    11941 tctgcaagcc atcgtcattt ctttgtttat ttatcatcga tcaagggatg cctatatcca
    12001 gctgttcaat caatcgaatg atgtggtact aaaaaagatt caatctgaat ttgaaagttt
    12061 gaatgataca atcgagaata cattagccgc ttttgattct aatccagctg tcaaaagcta
    12121 tttttccaat gatccagccc aacatatgga acagcttcag caactaagaa caatccaaaa
    12181 aatgaatgac tctctttcaa aaatccatcc aatgatcgat tacgatgtat tgatctttgg
    12241 agaaaatgga agaacttttg tcggaaacga catgctcact gccgtatctg ccgattcttt
    12301 ttttcaatct gcgattgctc agagagtgaa tgaacgtgct gctgatacac aaatgctttt
    12361 tgcacaccac ggattgactc ttcgcgataa gaaagcaccc agcgtcttct ttgtcaaaaa
    12421 actgaaaaat acattgaatc acgtttacgg atatgctgtt ttatcgatct catccaagca
    12481 actagcgaat ctttttcaat ctgtagtaaa tcctgagata tcgaagatct ccttcatgaa
    12541 cgaagaacag acgatcatcg catcaaatga agaaaataca attgggagcc gatcaatgat
    12601 tttcaaaaaa ctaacagtag gtgaaacaac gatttcaaat ggacagcagt tgacccgtct
    12661 tcctctgtac cgccaggatt gtacattact cagtcaagtc gatcttcatt ctttatctaa
    12721 ccaaatgggc gtgatttttc cgattatttt gtataatatc gtgacacttg cttttgctgg
    12781 ctcattggta tttatgtatt tggatcgaca cacaaaatcg atctaccgct tgattgattc
    12841 gctaaaacgg ctagaggaag atggcttaaa agctactgtt cctgtacaag gaagccatga
    12901 agtccaattg ctagggaata cgatcaatca attgctaagt aatgctcagc atcattatga
    12961 acagacgatc caagacgaaa agaagaaacg ttcgcttgaa atccaaacta tgcaagcaca
    13021 gatacagccc cattttattt ataatacgct gactagcttg aaatttttga tttggcagaa
    13081 aaaaacagat gaagcagttc aaggaatcga cagtttcatt actttactaa gaagtacgat
    13141 tgggaaaaaa gaagaagtga ttccagtcaa ggaagaaatc aaaagcgtac aaagttacat
    13201 cgatcttttg agtatgcgat atggcgaaag aatctcggct aaaataatga ttcctgatga
    13261 actgcagtca ttatctatgc cgaatatgat ccttcaacca attatcgaaa acgcctatct
    13321 tcatgctttt cacaataaaa cgcaaggatt catcacggtg tacggcaaaa caaaaaatca
    13381 gcttctttta ttcgaagtga ttgataacgg cgatggcttc gatactgaaa ataccaaaca
    13441 aaaaaatgat catttttcag gaatcggcat cagccatgta gatgagcgga tacgcctaat
    13501 gtatggcgat gcatatggct taacgatcca atcagtgatt gggacaggaa cgactgtaac
    13561 gatttgtcta ccgcttcttc ctaaaaatca atcagaatat caaaaataaa gaatagccaa
    13621 gcgctgattt ttttatttta agttaaataa atcaatcagt attcttgact ttcatctacg
    13681 cgtctcttct actgtcagca ctggataaca gcccaatatt tgactttctc ctccgagcat
    13741 cttgatttct tggagcgcat tctctactaa tgtcattgga cggtccaaca acaaatcgat
    13801 cacgaaaaaa tattctccca agctagtttt caatggtcgg gattcaatct tacttaagtt
    13861 gatttcccgc cagccaaaag ctgccagcat tttatgaagc atccctggcc gattagcagg
    13921 tagtgtcaag atgacactca tttttactgg agctgactgc tggctggtca tctttcggtc
    13981 accgacgatc caaaaacgtg tctggttcaa ctcattatct tggatattct cagctaagat
    14041 ctccaatcca acatgctgtg ctgtttctaa tgaagcaatc gctgctgcgt cttctttcgg
    14101 atgttctgct acatacattg ctgcagcagt tgttgattct gttgctacta aaggaacatt
    14161 aggataatgc gtttctaaaa actgttgtga ttgtgctagt gcttgtggat gtgaaaggat
    14221 ttttgtaatc tttgttgctg gattgcctaa aagctgctgc ttgataggaa gaacgatttc
    14281 acttttaact tcgacttctg gatgtttgga aagaagatcg attgtgtgat gaacagctcc
    14341 ttccaatgaa ttctctacag gaacgacagc cagatcgact tgtttgcgaa acaaagcgcg
    14401 caaacaaaca gggatggaag catacgcagt caattgttct gtaggaaata gctgacaagc
    14461 cgcctgataa gtgaatgaac tttctggccc tagatagctg acgttcatat gcttttgatc
    14521 ctttctataa tggtatcgat gatcttttct ggtgtttgat tggtcgtgtt gattcgatgt
    14581 gtcgcacttt cttcatacca agaaagtcgt tgtgccaata atgcttttat ttcttctctt
    14641 gtttttccaa gagctaaagg tcgaatagac gtttcatctt gttggatacg atcaactaga
    14701 cagtccgctt ctgcctctaa gtaaagaaca atcggctgtg tttttaaaaa acgccgattt
    14761 tctgcacttt ggacaatccc tccaccagtc gcgataattt tgtcttcatt tgacagcttt
    14821 cttaaaatat ctgactccac ttttcgaaag cttttttcac cgtaatagtg aaaatattcg
    14881 gcaatcgaca attggatatg ttcctcaatc tttgtatcta aatctacata ctctttttgt
    14941 aaacattgag ctaacccttt accgattgtt gtttttccag cgcccatgaa accaatcagc
    15001 aaaatgctag ccatgagctt ctcctcctga cacaagttct gctaaatcgt caaaaaatgc
    15061 cggataagag attgaaacag cttcagctcc ttctagttcg caaggtccat cagctatcaa
    15121 agcagctact tgcagcatca taccgattcg atgatcgcct ctgctggaca cttttgcgcc
    15181 atgcagaggt gtcggcccat ggatgatcag accgtctgct gtcgctttta tctttgctcc
    15241 cattttctgt aattcttctg ccaccgcatc aattctgttg gtttctttta cttttaattc
    15301 ttctgcgtct ttgatgatcg tgattccttc tgcttgagta gccactaaag ctagaatggg
    15361 caattcatca atcagacgcg gaatgatttc tccaccaatc gttgctttct tcaaatgact
    15421 cgtcttgaca cttaaatcag ccgattggtt ctgttcattg tgattcgtct gggtaatcgc
    15481 tgcgcccatc tcctccaaca catctaagat accagttctt gttggattga ccccgacatt
    15541 ttttaacaac agttggcttt ccggaacaag aagtcctgct gctagaaaga aagcagctga
    15601 tgaaatatca cctggaacag ttatctgttg accagttaat ttttgcggtc cggtcactat
    15661 gatcgtttta tcttccactg tgatccttcc gccaaattga cgaatcattt cttccgtatg
    15721 gttacgggaa cgttcttttt caacgattac ggatgtgcct tctgcttgta atgccgcaaa
    15781 cagcaaagca gatttgactt gcgcactggc tacaggcata ttgtatcgga taggtgataa
    15841 agagcgggtt cctgtcacag tgattggtag atactggtca ttttcttctc cttgcaagtc
    15901 agcgcccatc tcgcgtaaag gttccatcac tcgccccatt ggacgtttag acaacgaagc
    15961 atccccgaac agagtagtcg taaaaggctg tccagccaaa atacccatca ataaacgtat
    16021 ggtcgtaccg gagtttccca tgtcaattga ttcggttggt tggacaaatg agtggctacc
    16081 gcgaccatga atcttgatca cctcttcctc ttcttcgatc tcgacaccta attgttggaa
    16141 ggcatgtaaa gtactcagac aatcttctgc gcgcaaaaaa ttttgtatag tcgtcgttcc
    16201 ttcagcaatt gctccaaaca tgatgctgcg atgggaaatt gatttatctg ctggtatcct
    16261 aacagtccct cttaatccat gtatttgttg taataattgc atttatccag cctcctattt
    16321 gatccagcaa tgataactgg tattagacat aatgattctt tttgcttgta acaaatcctg
    16381 ttggttttta aaactgattt gcagtattcc taaaatatct tccctggtct cttggatctt
    16441 caaattaatc aaagaaactc ccgcttttcc gatcagtcca gttacttctg caataacgcc
    16501 aggttcatct ggcacatcga taaataaatc aaaaaaggca ggaatcgcgc cttgcttatg
    16561 gacaggcaac tggtctcgac tttcttttgc ccgattgaaa aaagaaaaga tttccgattg
    16621 gttctcagtc atgatccatt ctgctatttg tgtcatttct tgttgccaat ctgccaatcg
    16681 ttccaataat atctgcttat tgctcaacaa aatatctgtc cacatgacag gatctgagga
    16741 agcgatccta gtgatatctc gaaagcctcc tgctgccagt tgtttggctc gcggatactc
    16801 ttgattgaat tgttctgcct gattgactaa gccagctgcc agaatatgtg gaaaatgact
    16861 tagcattcct gttatacggt catgttctct tgctgtaaga gttacatatt ttgctttggt
    16921 tccgcaaaat agttcttgca gtttttcaac atcttttttc gtcttttcca aaggtgtaaa
    16981 gatgtaatag gcattttcaa atagtttagc atctgctgcc tgtatacctg atttatgaga
    17041 accagccatt ggatgtccac cgacaaaccg aagatttttc tgctcagcgg atgtcacgac
    17101 ttgctgcttc gtactgctga catccgtaat gatgacgtga tcagacaagt tgattctttc
    17161 aaggacatca agataagcta aggttgtacg gacgggaacg gctaataaga taacgtctgc
    17221 ttgttctgca cctgtttcaa aatcacttgc aacgtaatca acgatctgct gttctactgc
    17281 ttgctcacgg gtttttgact gactatccca accaactact atcagttctg gattggtctg
    17341 acgaatacaa agtgccatag aagcaccaat cagtccaagc cccacgataa aaacatgttg
    17401 tgccatctta tttgccccct ttttagtaat gtttcaagta gtcacggtac tccttcactg
    17461 cagctttcaa ctcaacaagc gaatcacttg ggaattttga caatagctct gtcgctacca
    17521 cagtggctac aacattttcc gcaacgacgc tagctgcagg aactgctgta ctatccgaac
    17581 gttctacact tgctttgtaa ggttcttttg tatcgatatc aacactttga agtggcttgt
    17641 acaatgtagg aatgggtttc atgaccccgc gaatgacaag tggttcgcca ttggtcattc
    17701 cgccttcgaa gccgcctaga ttattggtac gtcggaaata gcctctttct tcttcccata
    17761 ggatctcatc catcacttct gagcctggta atgaaccaga agtgaatccg accccaaatt
    17821 ctacgccttt aaaggcattg atactaacca cagcttgtgc gattttggca tctagcttcg
    17881 tgtcccattg tacgtagctt cctaatccag ctggaacgcc tccaacgagc acttctacaa
    17941 ttcctccgat cgtatctcca tttttctttg tctgatcgat caaatctttt attctttgct
    18001 caactgctgg atcaacaact cgtacttctg acgcttcaga aatttctttg atttcttgaa
    18061 tcgagatatt tccaggtatt tctgcttgga ttccaccaag acaaatcacg tgtcctgcta
    18121 cgatgatgtc aagttcttcc agaattttct tggcaattgc gccgattgct acacgcatgg
    18181 tcgtctctct tgcagaggac cgttcaagca cattccgaag atctcgatga tggtatttca
    18241 taccgcctac caaatccgcg tgtcctggac gcggacgtgc aactcgccgc agtgattggt
    18301 ctttttcagg cacttctgct acagacatga cagttgtcca atttttccaa tctttatttt
    18361 ctaccactaa agtgacaggt gaaccaattg ttttgccgtg acggatgcca gacgtgatcc
    18421 gaacacgatc tttttcgatc ttcatccttc ctcctctgcc gtaaccagct tgtctgcgag
    18481 cgagttcgtg attgatttct tcttctgaca gaggtaatcc tgctggtagt ccttcaataa
    18541 tggctgttaa ttctggtcca tgggattctc ccgcagtcat ataacgcatc ttacagtcct
    18601 cctaagtaat ctttgaatgt ctctgtggga acaggaacga tttttgcttg cccaatcttt
    18661 tctagcaaga tgatattcaa ttggcttcct cttgctttct tatcatgggt tatcgcgtgg
    18721 aataattcat ctggatgact tagatcgtac gtaatcggca gatggaattt ttgaagtatc
    18781 ctttctaaag cagctgtcga tccagttgga gacaagccga ttttttctgc atgagaagtg
    18841 atggctacca ttccaatagc tactccttct ccatgactga ctaccccata tcctgctgta
    18901 ttttcaattg catggccaat tgtatgacca aaatttaaaa taagtcgatt tccttggtca
    18961 aagacatctt cttccactac ttttcgtttg atttccaacg tagcagtaat gattggaaca
    19021 gcatgttcca gaagatcttt ttcgtctttc agctcgttca gctgtttcca aagcgttgca
    19081 tctgctatag ctgctgattt gatgatttca gcgattcctt ctcgtaaacg acgtaccggc
    19141 aaagttttta gaacctcagg atcaatcaat acaccagctg gctggctaaa tgttccgaca
    19201 aggtttttcg ctttgtttgt attgactgct gtcttcccgc caatactaga atctacttgt
    19261 gcaagtagcg tagtcggaat ttgaacgaaa tggatcccac gcatgaacgt cgaggccacg
    19321 aatgctgcta gatcacccac cattcctcca ccaagtgcaa gtatcccatc gctgcgggta
    19381 aaatcattat ctgctaaaaa atcgtataac tcagctgctt gttctaaaga tttactggtc
    19441 tcacccgccg gtacgattga aaggactggt tcaaaattag cttgtctcaa ttggaacatg
    19501 attttttctg catatagtgg agcaacgttt gtatcagtga tcacagctac tttttgaggt
    19561 ctccaaatcg tttgaagcca tgatccagcc tgttctagtg cgcctttttc aatatgaata
    19621 tcgtattttt ggttatcaac atgtacagta agcattcgtt tcacttcttt cttttaaaaa
    19681 gctggaatta atcgagtgtc tgaatctttt ccattgcttc tttcatgatc gcaacttctt
    19741 tcatcatgcg tgagtaagtc ttttcattca acgattgggg accgtctgac caagcatttg
    19801 caggatcagg atggatctct acgatcatgc catctgctcc gcttgccaca cccgcacgag
    19861 ccattggtgg tacaagttcc caaattcctg taccatgact tggatcaaca atcacaggga
    19921 aatgagttaa ttttttcatc aatgggacag cacttaaatc aaacgtatta cgtgtcgctg
    19981 tttcgtaagt ccgaatccca cgttcgataa aaatgacagg agatttgtct tcgactgcaa
    20041 tgtattctgc agcattaagc cattcattga tcgtaccgga aattccacgt ttcaatccaa
    20101 caggtttacc tgttttaccg acagcagata gcagtttaaa attttgcata ttacgtgcgc
    20161 caatctgtag gatatcgctg tactcagcca ccatatcgat atgcccttca tccatcactt
    20221 cagtaatcac tttcattcca aattcatcag ccgcttgacg gatgtatttc aatccttctt
    20281 cttctaaacc ttggaaagca tatggagaag tacgtggttt gaaggcacca cctcgcagaa
    20341 tttttgcgcc cccggctttg gccattcgcg cacattctct gatttggtct aatccttcga
    20401 tagaacaagg accagccatc gtcacgaaac tgccgtcacc aatttttact ccgtctacat
    20461 ccacgactgt attgccagga tggaattctc gagaagtcaa tttgtatgtg tttaaaatac
    20521 gcacagcatt ttctactcct tcatagcgtg taaatgctcc ttcttgtaaa cttcgcgtat
    20581 caccttttag tccaatcact actcgttctg ttccttgatc gattgagact ttgaatcctt
    20641 ctttttcgat atgatcgacg acttttttta tttgttcttc cgttgcttct tttttcataa
    20701 ttactatcat tgtttttccc gctttctttt gtttacttat ctatcttggc tgattttctc
    20761 tactttttca aggtggtttt acattaattc ctactaaatg accgtgtcct gttgttctca
    20821 ttttcgctcc ttctttccta cttatgaccc agataatcaa caaaaaaaga tgtccctgta
    20881 ggaaattgcc tctacagtga acatcttcaa cgagccttaa aaagcgctgc aaaaatataa
    20941 aatagccact atagatgaat ttctctacgt ggctgtaagt cgtgtttaaa aatctttagc
    21001 catcatagat acaaacctgg tttgtttgta cccacgaagt catgaataca aacctatcta
    21061 aaatagctaa agtaatacgt attcagtttt gtaagttttc tagatttcat aaaaaatcgc
    21121 tccaaactga tgtaagaatt gt
//
LOCUS       DAZAFF010000045        20945 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000045 DAZAFF010000000
VERSION     DAZAFF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.045"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACG9BB_001586"
     CDS             591..1280
                     /locus_tag="ACG9BB_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFN2837530.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACG9BB_001587"
     CDS             1267..2475
                     /locus_tag="ACG9BB_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN2837531.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACG9BB_001588"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACG9BB_001588"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFN2837532.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG9BB_001589"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACG9BB_001589"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFN2837533.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACG9BB_001590"
     CDS             complement(6578..7273)
                     /locus_tag="ACG9BB_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFN2837534.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG9BB_001591"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACG9BB_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFN2837535.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACG9BB_001592"
     CDS             complement(8472..8663)
                     /locus_tag="ACG9BB_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFN2837536.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACG9BB_001593"
     CDS             complement(8684..9574)
                     /locus_tag="ACG9BB_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFN2837537.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACG9BB_001594"
     CDS             complement(9561..10328)
                     /locus_tag="ACG9BB_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFN2837538.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG9BB_001595"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACG9BB_001595"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFN2837539.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG9BB_001596"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACG9BB_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFN2837540.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG9BB_001597"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACG9BB_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFN2837541.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACG9BB_001598"
     CDS             14899..15282
                     /locus_tag="ACG9BB_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837542.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG9BB_001599"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACG9BB_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFN2837543.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACG9BB_001600"
     CDS             complement(16226..17044)
                     /locus_tag="ACG9BB_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFN2837544.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG9BB_001601"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACG9BB_001601"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFN2837545.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG9BB_001602"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACG9BB_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFN2837546.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACG9BB_001603"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACG9BB_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFN2837547.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ACG9BB_001604"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ACG9BB_001604"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFN2837548.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5988 a         4261 c         3838 g         6858 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaa
//
LOCUS       DAZAFF010000046        20887 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000046 DAZAFF010000000
VERSION     DAZAFF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.046"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            193..1395
                     /locus_tag="ACG9BB_001605"
     CDS             193..1395
                     /locus_tag="ACG9BB_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFN2837549.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            complement(1572..2840)
                     /locus_tag="ACG9BB_001606"
     CDS             complement(1572..2840)
                     /locus_tag="ACG9BB_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFN2837550.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     regulatory      complement(2891..2958)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(3001..3648)
                     /locus_tag="ACG9BB_001607"
     CDS             complement(3001..3648)
                     /locus_tag="ACG9BB_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFN2837551.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     gene            complement(3913..4416)
                     /locus_tag="ACG9BB_001608"
     CDS             complement(3913..4416)
                     /locus_tag="ACG9BB_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN2837552.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            complement(4540..5304)
                     /locus_tag="ACG9BB_001609"
     CDS             complement(4540..5304)
                     /locus_tag="ACG9BB_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFN2837553.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            5412..5687
                     /locus_tag="ACG9BB_001610"
     CDS             5412..5687
                     /locus_tag="ACG9BB_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFN2837554.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            complement(6035..7186)
                     /locus_tag="ACG9BB_001611"
     CDS             complement(6035..7186)
                     /locus_tag="ACG9BB_001611"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN2837555.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(7206..7928)
                     /locus_tag="ACG9BB_001612"
     CDS             complement(7206..7928)
                     /locus_tag="ACG9BB_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN2837556.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            complement(7921..9372)
                     /locus_tag="ACG9BB_001613"
     CDS             complement(7921..9372)
                     /locus_tag="ACG9BB_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFN2837557.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            9514..10596
                     /locus_tag="ACG9BB_001614"
     CDS             9514..10596
                     /locus_tag="ACG9BB_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN2837558.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            10930..11868
                     /gene="yidC"
                     /locus_tag="ACG9BB_001615"
     CDS             10930..11868
                     /gene="yidC"
                     /locus_tag="ACG9BB_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFN2837559.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(12049..12453)
                     /locus_tag="ACG9BB_001616"
     CDS             complement(12049..12453)
                     /locus_tag="ACG9BB_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2837560.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(12469..13278)
                     /locus_tag="ACG9BB_001617"
     CDS             complement(12469..13278)
                     /locus_tag="ACG9BB_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN2837561.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(13265..14155)
                     /locus_tag="ACG9BB_001618"
     CDS             complement(13265..14155)
                     /locus_tag="ACG9BB_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN2837562.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            complement(14168..14647)
                     /locus_tag="ACG9BB_001619"
     CDS             complement(14168..14647)
                     /locus_tag="ACG9BB_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN2837563.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            complement(14664..16430)
                     /locus_tag="ACG9BB_001620"
     CDS             complement(14664..16430)
                     /locus_tag="ACG9BB_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFN2837564.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            complement(16476..17417)
                     /locus_tag="ACG9BB_001621"
     CDS             complement(16476..17417)
                     /locus_tag="ACG9BB_001621"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFN2837565.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            complement(17414..18409)
                     /gene="lacD"
                     /locus_tag="ACG9BB_001622"
     CDS             complement(17414..18409)
                     /gene="lacD"
                     /locus_tag="ACG9BB_001622"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFN2837566.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            complement(18402..19595)
                     /locus_tag="ACG9BB_001623"
     CDS             complement(18402..19595)
                     /locus_tag="ACG9BB_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN2837567.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            complement(19613..20341)
                     /locus_tag="ACG9BB_001624"
     CDS             complement(19613..20341)
                     /locus_tag="ACG9BB_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2837568.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
BASE COUNT         6072 a         4479 c         3644 g         6692 t
ORIGIN      
        1 ctaaaaaatc tgttaatttg aagatttatg ttttcgataa aagctttttt caacataatt
       61 cgttataata gaaacgtcgt gaaaaaactg attgactatt tagtaactaa cttttctata
      121 ttcgatatat ttactactaa atgatgctat cttttttaag aaaaaattta caaatcgaaa
      181 ggagttcatt caatgaatct tactagagac ctgatatttt tagttatcta tattttagga
      241 atgtcaggca tcctctattg gcgcaagcgc caaagcgatc ccgctctatt cttttattgt
      301 gggacgtgga cttttatgat gatggtcact tacttcgtcg ttttagaagt tttttatcat
      361 acattttatt ttttgattcc attgctattt ttcagtattt ttgcattttt ctattttaag
      421 gaaaaacgta ggttaatcaa tggcgtactt tttaatacgt ttttgattgt ctttggtggt
      481 tatctagggt ggctactaat agagacgcaa aatatcttta tcggtgggtt attggctttg
      541 atcgggatac caatattaat tcttttggca atcggtatct acattttgat cattttttta
      601 ttttggaatg gtgttgtagt catgaaaaaa gaatcccgtt ctttggccaa ccttttgact
      661 ctcttgttag gagtcgcttt gactatcttt ttgatattgg actatacatt agtccattgg
      721 ctcccacaat gggcaaatgc tctatttgct gtcattccgt ttatttttgc ttatctactc
      781 atcgttttct ttaattttat gactgtttca attttgtatc agttcaaccg tccaaggtat
      841 aaccaagatt atatcgttgt tctcggcgct ggcttgattg atggtgaacg cgtatctccc
      901 ctacttgcta aaaggatcaa taaggcgatc gcattttatt gggcacaaag taaagcaact
      961 ttgaatccgc cagtcttgct catgtcagga ggaaaaggaa ccgatgaaaa agtagctgaa
     1021 gccattgcaa tgaaacaata tgctttagaa aaagggattc ccgagcgtga tattttagta
     1081 gaaacgaatt ccacaacgac gctagaaaat atgctgtttt caaaagaaat aatggatcag
     1141 caaatgaatg ggccttatcg cgcgattttt acttcaaata attaccatat ctttagagct
     1201 ggactatatg cgcgacaagc gaaactcaat gcaaacggaa tcggtgcaaa aactgcacta
     1261 tactacttac cgaatgctat cttaagagaa tttattgcca tccttgtctt acacaaaaaa
     1321 cgccatctta tcatctgtgg cttaatgatt ggctttgcag tattttctgc tcttattctc
     1381 aaatttgctc aataaaaaag agcgtgggac aaaactaaaa atcagttttg ccacagactc
     1441 taactccgaa taaacggtgg gagcagaagc aactccttcg gaaataagcg aaattcacaa
     1501 aaatttaaaa agcaattttc gtgaatttct tcttatttct cggagttaaa cacttctgtc
     1561 ccaacctctt tttatttctc tttttgtcgt gagattcttc caaggatcgg taggaacaag
     1621 attccttttt tcacttcaac tttttgcgca gaatagattc cactatttcc tgaacacata
     1681 aagctaatca aacagatcat aaagaagtag ctggctgctt gacctccaaa aagttcgatc
     1741 cccataacaa agcaagcaat cggtgtattc gttgctccag agaacacccc aataaaccct
     1801 aatcctgcta aaaaaggtac tgatacatga agaatgggtg ctaatgtcgc acctaaagtt
     1861 gccccaatct caaacaaagg ggtcacttcg cctccttggt atcccgcgcc aagtgataga
     1921 acggtaaagc caagtttccc aagaaaatca taaggatgag cacttccatt aaatgcatct
     1981 tgtaataaag gtaagcttaa tccgagatat ctttgcgtct gtaaaatcac tactacgagt
     2041 accacgactg ctcctccaat aaagttgcgt aaaactggat gggggatccg ctttgcatac
     2101 caagctttta accagacgat cgaacggcta aagagccagc caatgatacc aaatgcaatg
     2161 cccgcaagca acaatttgaa gaaaagtgaa gggtcccatg caggaatcgc ccccatttga
     2221 taatgggtat gcttgacatt gaaactttcc gttacaaagt tcgcaaaaag tgctgcaaaa
     2281 aagcttggaa acagtgcttc ggttctcact tttccgattg ccaatacttc taatccaaac
     2341 aatgtcccag ccaagggtgt cccaaatact gagctaaaac ctgcgctgat cccactgatg
     2401 acaagaatct cacgctcgat tttgttcaat tttagccaac gagcaaaatt ctcagcaagt
     2461 gccccgccca tttggaccgc tgtaccctcc cgaccaacag aaccaccaaa aagatgcgtg
     2521 gtaatggtgc caaataaggt cagtgggatc agccgtaatg gaatcttttc atttcctcca
     2581 ttcccttgat cgatcactaa attgtttccc cgggaagaat tgcccccata cgtcgtatat
     2641 agataagcaa aaagtccccc acttatcggc aaaagaaaaa gtaaccaagg atgctccagc
     2701 cgaatagctg tcacatcagt caaactagtt agaaagaatg cggataaact tcccataaaa
     2761 atacctaaaa ttgctgtgat caataaccat ttgatcatat agatgatcac cttcaatgga
     2821 tcaggtaatc gattttccat ctcatttcct cctcaaaaaa acgcctacta aaaaaagggg
     2881 tagccccgtt tctttagtag gcgtcattag ttatcttacg atcaacgttt aaaggcgaac
     2941 accatcacct attcacttca ccaaataaga tagcatattt ttcagaaaaa tacaatgaaa
     3001 tcaccgctca cgtttcactc gttctgtgaa ttcgtaaaaa ggagctaatt gtaccaactg
     3061 ctgattacat tggtcactat catgacaaat ataatttcct tttttcgtgt atgttccatc
     3121 tgaaccagtt tttgtggtag aaagaaagag tgaaacattt gaagttcttt gacaaatcga
     3181 acaaattcct ttgttgattg taggagataa tgttccactg atccctttta gtttttcttc
     3241 ttcataatag atcataaatt ttttttggct acctggatca ttccaaccaa tataagtatg
     3301 ttcccttaaa tccagttgat tccaatctgg ttgttttaac tttttcactt ttcggaataa
     3361 tttatcgatc tgcggttttg tcggttgtat gaatggaaga acgatcactt tcaattcctc
     3421 taatagtcgt tctgcttttt ctttttttag agtgacgtct agtacctttt ccataaattc
     3481 atttacagct gtattaggat gaccaagtac ctcgtggatc ttttccgcag ctaatgcttg
     3541 gactgtttgg actgtctttg gatcattcac tgaactatag gcgcttaaca gctgctcgac
     3601 ttctcgcttg ataaaataat attgatatgg tttgattgtt ttttccatga ctttgagtcc
     3661 tttcttcttt caaaatcggc tactttgaag aaaaaagtga tctttctatc aaagtgcctt
     3721 tagataaagc cctcaaactc ttttaaataa gatacacatc gcatcttatc atcaccccct
     3781 ttatttttat gttggttatg tactttttca tctcaacaaa gtaaacacta aaaagaccaa
     3841 ttagtcaatg tctaaacaaa aaacccgagg atttttttca aaatactcgg gtcaaatact
     3901 tagttaacta ccttaaatcg attgttcttt accgaaatat cgttgccatt tttgtttcat
     3961 tgaagctgtc attggttgag ctgcttcttt gactgcacaa tatttatcca caaaagtaac
     4021 gatataacct tctttatatt taggaggggc aatcgttgct ccccacatat gtttcaaaat
     4081 aatatcacgc tctagatctg aaagctccgt gattttttca gcatttttca cagcgatcct
     4141 tgggtgaaca tacgcatgtg taccttcatc gaacttcgtc gtacgccaat cgtagtaaaa
     4201 taaatcatgt aaaaggcctg ctcgagcagt cgctcttgca tcaccgcccc attttttcgc
     4261 taatttgtag ctatagtaag acacactgat tgaatgctct aatcgtgtcg agtggacatg
     4321 ttgcgtgtaa tttgctaatt tctttactgc atcagtttct aatagatcat ttatataaga
     4381 catatattct tcgtcttcac gccatttttc tgtcataatt atcgatctgc cacctttttg
     4441 aattccattt atctttgaaa ttgaacttag tataccacta gtgttcgtaa tattctagtg
     4501 aggttttcgc aaaatgcaga aagcttaata gttccttaac tatacggaga agtgataaag
     4561 catgatgccc gctgcaactc ctacatttaa agactccgca tttccttgaa tcggtatata
     4621 cacattttta tccgttagcg ccagcacttc tggacgtacg ccttgacctt cattccccat
     4681 cacgatcgca taattttctg ttgtctggat atcattcagg gacactgctt cttcattcag
     4741 ttctgttcca taggttataa atccttgctc tttaaggata ggcaatagtt ttgttagatc
     4801 ctcttggatg atcggtaaat gatagttact cccctgcatg cttcttaata ctttggtact
     4861 gtagacatcc gccgttccct tacctagtac aacacaagat agaccaaaag catcggctgt
     4921 gcggatcatt gtccctacat tgccgggatc ttgtacgttg tcgagcaaca accaaccacc
     4981 ttcaaaattc ggtatctctc ttttttcagc ttccaccact gccatgatcc cttgcggagt
     5041 tggtagatct gaaacagttg ccatcacttc ttctgatact agtgtaacag aaagcgcctg
     5101 attttcttca agccaactag cccactcagt cagaccgcgt tgcgtaataa aaagttcgtt
     5161 gaccttcgca tttgaggcaa cagcctcctc gatcaggtga aacccttcaa gcagatattc
     5221 ccctttttct tcacgatatt ttttcttatg taattttttt aattctttga ttcttggatt
     5281 ttttgctgat tggatctctt tcacattttt cacctcaact gattatacca tggattagat
     5341 cattgcactt gttcagtagc aaaaaagtcg gtatcattaa tttaaaaata aaagaaagaa
     5401 cggtgaataa aatgaacaaa atcaagatga acgtgcaagg acgtgtacaa agcgttggtt
     5461 ttcgctatat gacaaaaatc gtagcggatc aattagggat caccggaaca gtgaaaaatg
     5521 aagatgacgg ctctgtcacg atcgaagctg cgggcgacga tgaggcaatc aaaaaattcg
     5581 tggaagaagt caaacgatca ccaagcccca gcggacgtgt ccaatatgta gaccttcagg
     5641 aaaatccgat gatggaggac agaaaaaagt ttgttgtaat aggctgacct tctagaaatg
     5701 aataggaaat gtcagaaaaa tcggactgtc tgtatgtaga tcgaaaaagt ttgcccctct
     5761 ctccatccta gatcgacaaa aaaacattct tattagcgag tttagtaacc agtgataggc
     5821 atacgtaaaa gcgggaggac ttcaaaaatt ttggttcaat aaaatggagt ctataatatt
     5881 ttatggtagt ggaaaatcga ctttgatttt ccactaccac tttttcattg tcaaatcctg
     5941 acgctgccca gtcaacaagc caactctcga aaggatcatt ttccctcttt gttctaatgg
     6001 atacccatag ctgttttcaa atatacagtt ttatctaaaa atttagtttt gctaaacgat
     6061 caaaggcttc acctaagatt tccaatggct gagtcgctgc tagtcgaata tagttgtccc
     6121 cgatcgtttc cccaaacgct tttccaggga caaaaagtac gccgcttttt tctaaaacct
     6181 cttccacaaa cgttactgaa tcgagcccac tcttactgat attaataaaa gcatacatgc
     6241 ttccagaaac agaagataaa gataagtaag ggatttctgc cactcttttc tcaatataag
     6301 ctaatcgttc tttgaatact tcaacaactg gcggtacaag ctgttcatag tgctttaagg
     6361 cgtagattgc acctctttga gaagcagtcg gtgcggaata agtgatgttt tcattgatca
     6421 acccagcaat ttgattgatg tatgctggtg cgatcatgta ccctaagcgc cagccagtca
     6481 tcgcaaaccc cttcgataca ccactgacag tgatcgtatt atcgggcgca aatcgcgcca
     6541 tcggtacgaa cttctcttta aaggcaaagt cttcataaat ctcatcggaa atgatataga
     6601 gttcattttc gattgcgact tcagcgattt ttttcaacgt gctttcagaa aaaacagctc
     6661 ccgtaggatt attcggcgaa ttgatgacga tcacctttgt acgatccgta atcgctgctt
     6721 ttaaatcttc cacatccaac tggaagccct tttcttcata ggtaggaaca aaaacaggct
     6781 tgcctccaga taaaatgact tgttcccgat atggtgaaaa ataaggttca tggatgatga
     6841 cctcatcctc tggatctagt aatgcctgta aaactagata catcccgtgc attgctccga
     6901 ctgtagctcg aacttggttg cattcaacgc tcaatccata tttcttttga taaaaatcta
     6961 cgaccgttga caagaattct ttgctgccat caggcgcagt ataattagta gcaccatttt
     7021 ttacatctgt ataagctgct tcaataatcg tcggatcagt gatcaagtca ggatcaccaa
     7081 tcgataaatc gataatatcc tctttttcct ttgccaaaat agcaatattc atcaacagat
     7141 tttctgctgg ttgctgaaac cggcgtgcaa tcttactttt gtccatctta cttcctccat
     7201 ctttgttaca acactttgtt caagaaatcc tgtgttcgac tattttgcgg attttcaaaa
     7261 atctctttcg gtgttccctc ttcttgaatg atgccgtcag ccataaaaat cacacgatcg
     7321 gcaacttctt tagcaaaacc catttcatga gtgaccacga ccatcgtcat ccctttttta
     7381 gctaaatcct gcatgactgc caacacttca ccgaccattt caggatcaag tgcagatgta
     7441 ggttcatcga aaagcatcac atcaggattc attgctaatg ctcgagcaat tgctacccgt
     7501 tgttgctgtc ccccagataa actggtagga taactgtcca ctttttcctt tagaccaact
     7561 tggttcaata atgatagagc atgttctttt gcttttttag ggtcttcttt tttgattttt
     7621 acaggtgtga tggtcaaatt ttcgagtaca ttcatatggg gaaacaaatt aaaactttga
     7681 aataccattc ccattttttg acgaatttga ttgatatcgc tgtcaggggc tgtaatgttt
     7741 tgtcccttaa aaaagatttc accagaagtt ggttcttcca gcagattcat acaacgtaga
     7801 attgtacttt tcccacttcc tgaagggcca atgatcacca ctacttctcc ttctttcaca
     7861 gaaagatcaa ttgattttaa gacttcattc tcgccaaaac tttttgacag tttttttaca
     7921 ttaagcattc agacccattc tcctttcaac taaagctaat atacgggaaa tactgaaggt
     7981 caacacgaaa taaatcaaag agaccaacac atatggtagg aatggtttga agctagcacc
     8041 ttgtaccacg cccgtttgaa agatcaattc cgatacccca atgaccgaaa caactgagga
     8101 ctctttgatg atcgtgacaa actcatttcc tagagctgga agaatatttt tgattgcttg
     8161 tgggaagatg atgaaacgca tggcttcttt ctggtccatt cctaaagaac gtgcagcttc
     8221 catctgtcct ttgttgaccg cattgatccc ggcacggaca atttcggcta cataagcagc
     8281 actattcaac gagacagcaa tacaacctgc taccagtttc gatagatcca atcccagtac
     8341 tcctgttccg aagtaaacga taaagatttg aactaagaga ggtgttcccc gtacgtattc
     8401 gatataaata atggctaatg cctttaaaaa tttattctta cccaatttca tcagtgcaag
     8461 caaactacca aagaccattc caaacaatac acccacaaaa gctaataaaa tagtgtattt
     8521 tgttccaatc aaataatacg gaccgtattt ttgaagaaag gactgatcat cttggaacat
     8581 caattgattc gcttgttttt gataatcttt cagcaagccg tcgtcattga tcgttttgat
     8641 tgacgcattg acatgatttt tcagttctgg tgtatttttt gacatcgcaa tagctgtggt
     8701 tttttcgcct tgcgcaaatt ttactggagt caaagctact ttcttattct gttgggcata
     8761 tgcttctgca actggttgtt ccagcacaac accttcaact ttttcattca gcaaattatt
     8821 gatcagatca ggaacttttt gcagggaggt caactctgca ccagtcagtt catttttcac
     8881 taactcctct tgtgtggttt gcttttgcgc cccaacggct aagccagaaa agtcggctgt
     8941 cgtcttaaat ttatttttgt cctctttacg aacgagtact ttttgctgta ccacgaaata
     9001 tgggtcagaa aagtcaactt cttgtaaacg ttcttcagtc accgccatac cagatataac
     9061 taaatcgatc ttgccagttt tcaacgcacc taataatgca tcaaaaccaa gttcttctac
     9121 ttttagtttc acacccatat cctttgcgat cttttcggca atggatacat caaaaccaac
     9181 gattttatcc ttaccatcca cattcgcatg gaattcatag ggcgcatagt ctgcagatag
     9241 gccaacgact aattctccac tttgttttat ccgctcgtaa atttcatctt tttctgcacc
     9301 ttgtactttt actggtaaaa aaaccagtag aaacatcagc gccactaata atatgtttat
     9361 tccttttttc atccatttgg ccacctttga ataataatgt aatcagaaaa gctataaaag
     9421 aataaatatt cataaataac ttgctaatat actagcataa ctattataat aaaaaaagat
     9481 taatttaaaa aaataatgga agaagttttt tatatgacaa aaaatttaat tgttcgcggt
     9541 gcccctcaag agtatgaaat aaaagaaaac gcatgggatt cactccctga acacctttac
     9601 aaaaggcata ttgacaacgt tttgattctc catggaaaaa catcttggaa agcagtaaaa
     9661 gatgtttttc cagatttatc aataacaaaa atttttgaat attatggtgg agaatgtacc
     9721 gatccacttg ctgacagttt tgtttcttta tgcaaaaaga aggatttcaa tggtattatt
     9781 gcagtcggcg gcgggaaagt agcagatcta ggaaaattga ttgcctataa gacaaactta
     9841 ccaatcatca tcttacctac cttagctgcg acgtgcgcag cttatactcc acttagtgtg
     9901 gtctataatg aagaaggagt gatgcttcgc tatgatatgt tccctaaagc aaatgattta
     9961 gtgttgatcg atccaaagat cattttgcac tcccctcgcg agatgatgat tgccggtatt
    10021 ggtgacacct tagcaaaatg gtatgaatct cacgcaatca tctctcagtt aactatcaag
    10081 cctatcgaag tccaagtagc ggaatttgca gcgttacgat gccgcgaaat ccttttagag
    10141 aaaagtgcag aggcaatgga agcgatggac cagcaaaaaa tcaatcaagc ttttatcgat
    10201 gtcgtggaaa caaacatttt attggctggt atggttggtg ggtttggcga tgactacggc
    10261 cgaacagctg gagctcattc cattcacgat gcgatgaccg tactccctga tagccatcag
    10321 caacttcatg gaaacaaagt tgcatacggt atccttgtcc agctgatgat cgaaaataaa
    10381 ttagcagaaa tcgaacacct actgccgttt tatcatcact tgaacttacc aacgagttta
    10441 gctgatatgg gtttacattt aacagaaagt gactatcaag ccgtagctga acgtgcatgt
    10501 ttacctgatg aaatgatcca cttcatgaaa gaaacgatca caccagcact ggttgtccag
    10561 aaaatgaaag agttagaaca agtgacacag ggatagttag ccccttatac catggcagtc
    10621 agctttttta tggatcggct caatggtaaa aaaaaagaag tggaaaaaat tgacattcca
    10681 cttctttttt ttgcgctaaa attatgattt caaggaaata tccgttgctc atcttatttt
    10741 agagaacgga ggtgctaagt atgctttata ctgaagtatt tttagctgga ttcttctttt
    10801 taacattaag cgtctttttt gatcctccaa tcggttattt ctatgaaatt tctatgaagt
    10861 tgattgaatt atgagcgctt ttttagtata gttaagcttg tgtaaaaatt ataaaagagg
    10921 aagtatttta tgaataaatg gaaaaattgg ttattgggct ctgggcttgt cacgatcatg
    10981 cttttcttgt ccggttgtgt ccgcatggat tcaaatggta accctgacac ttcaggaatc
    11041 atttaccgta tattggttca cccaatggga caagcgatta catatttagt ggaaaacttt
    11101 aattggtcct acggctgggc tgttatcttg atgacagtca tcgttcgtat catcattttg
    11161 ccattaggta ttagtcaatc gaaaaaaaca atgatccaat ctgaaaagat gcaagcattg
    11221 aaaccacaag ttgaagctac gcaacaaaaa ttgaaagctg cgacaactcg agaagaacaa
    11281 atggctgcac aagctgaaat gcaacaagtt tatcgtgaaa atggcttaag tatgactggt
    11341 ggtatcggtt gtttaccgct tctgatccaa atgccgatct tttctgcgct atatttcact
    11401 gctcgctata cagaaggcat ccgtgaatca accttttacg ggatcgacct aggtaaccct
    11461 agcttagttt tagtagcgat tgccggtatt gcttatttac ttcaaggcta tatctcaact
    11521 atcggtatcc ctgaagaaca aaagaaaacg atgcgtacga tgttgatcgt atcacccgct
    11581 atgatcgtct tcatgtcgat cagtgcccca gctggtgtca ctctttactg ggtagtcggt
    11641 gggatcttta gttgtctaca aacattcatt acgaatgtca tcatgaaacc aagattaaaa
    11701 gcgcaagttg cggaagaatt aaaacaaaat ccgccaaaac aagtggtgac gcctcgtaaa
    11761 gatgtcacac ctgctgaacc tgcgaagcaa acaaaaaaac aagtacaagc accaaaaaac
    11821 aatgcgaaga atggtcggaa tgctggtaaa cagcaaaagc gtaaatagtt atttgtaact
    11881 agcttcttcg atcgacccag atcatttctg attttatgac gataaaagcc aacgtactgt
    11941 gaaatataga aaactatatt tcgcggtacg ttggcttttt tatacacggc ctctttatac
    12001 atccgtattt atatccagtt gtatcaccag tcaagaagtt gtgcttgatt attcttcttc
    12061 atcgtctccc atgccagcaa tcacttggtt cactgaaacg atactctcac gacccgaacc
    12121 aataaaatct ggttctgctc caatcatttg cgaattgatg aattcgatga ccatcggcaa
    12181 attcatgcca gcatatagtt cgatcgtttc accttccatg attagcttgg aaacggtatt
    12241 tgcaggtgtc ccacccaaca gatcgcaaag cactacgtaa tctttcaatg gtgctgtaac
    12301 ggagaggaat ctttctttat acgtgtccgc gccatcttca ggcaataaag ggatcgtata
    12361 gatattttct tgtggtccca tgatcatttc tgtactgttt tttagttctt cacaaaattt
    12421 accatgacta atcaagacta gttgtctact catctttcca attctccctt atttcgaaat
    12481 cacacctaat gcagataatg ctacacatac gatcaatacg atgaatattg ctcgagtagg
    12541 tgtcatattt ttcttaccta acatccaata aacgccacca acgattccag ctggtaaaag
    12601 tttaggtaag atcatatcga tatagttttg catattcaac gttacatccc caatcgttgg
    12661 tgcccatgag aatttaacat tgatcatggt tgctactaac gctccaacca tgaaaactcc
    12721 tagtaaagta gccgcatctg tcaatgcagt caagcgtgat tgcatcgtgg taactagaga
    12781 aatcccttct ttgtaggcaa attctagttg tttccaacga aagaccatga cagcgacctg
    12841 agcaaggatc cagataagga tacctactgg attaccatct gttgccatat ttgcggctaa
    12901 agctccaaat atggtcggga tcaatgccgc aaatacagaa tcaccaattg cagcgaagga
    12961 acccatcaat cctgctttca tcccagaaac agtttccttt gaagagactc cttctttttc
    13021 ttcgatagca agatcgatcc cagtaatgat cgtgttaaag aaattagacg tattaaaaaa
    13081 ttgcgtatgt gttttcatca tttcttttaa ttctggtgta tcatcaccat agatttttct
    13141 aagttgtggc aagatagtat acagataacc tgaaccttgc attctttcat agttccaacc
    13201 taattggaaa gtaaacaaac ttctacgatt gatctgctta aaatcttctt tggtcaattt
    13261 ataattagat ttcgtcatct tcgatttccc cctcatcaga ttgtgattgc tgacttacta
    13321 ctggatgttg gtcattcttt tgatcattct tataactgat cgttgccagt gctagaccaa
    13381 tcaatgccac agcaagcatc ggtaatgaat tgaatgttcc tgggaaatct ttgaccacgc
    13441 tagccacact tgttcctaac aattggacat tcgagaacac tgttgctaat aacgctgtga
    13501 tcgtaaatcc taaaatcaga taagaaatgt gtttctttac tggtaggtag cgtagtaaaa
    13561 ttgcaaaccc aactgctggt aagactgccc ctgcaacaga taagccatct cctagccatt
    13621 ttaggtcgcc gtttaaaaca gccacaacat tttggactaa gccaccaccg aatgctaggg
    13681 ccaagaatac aggaaccatt ctggaaagtg cccaaggtaa tgcccccatc aagaagttcc
    13741 gttcaattgc tttgtaattc attgtttcca catgttgatc gatccgatgt gcaaaatacg
    13801 tattcgcaaa acgtgccaaa atatctaatt ggatcatcaa acttgctaca ggaaccgcga
    13861 tggccgcaat cgcttgttct gggttcatcc ctagagaaat ggagaaagct gttgctaaga
    13921 tcgttccaga gttggcatcg atttttgatg caccaccaaa tgtccctacc cctaaaacag
    13981 tcaactgcat acttccacca atgattaaac ctgcttttaa atcacccatt actaacccag
    14041 catataatcc tgcaaaaact ggggcgctca ttgaagaata aatctgtaat tcatctaaaa
    14101 tttgataccc tgcatataat gttaataata atatctgcca ccattgaata tccatgaatc
    14161 attcctccta tttgattaaa agactcataa agtcttctgc tttgtcattt ggcaccattt
    14221 gagcgatcaa atgaacgcca agctgatcta atttcttgaa gacctctaca tccgcatcta
    14281 cgacattgat tgattttgtg atggaacgtg tttcatctgt ttgtgacata ttgccgacat
    14341 ttaattcttt gatcggtaca cctttttcta ctagcgctaa gatacgatct ggttttttcg
    14401 ctacgatcag cactcgttga gaatcatatt tacctgctaa aatatttgcg gctgcttttt
    14461 caataggtaa aatacttaat ttgactccag ctggcgttgc caactttaac ccactttttt
    14521 cgatcgcatt tgttgctacc tcatcgtcta cgaccatgat ccgaccaata ttcaactttg
    14581 tcgtccaaag atttgccact tgtccatgta tcaaacgtcc atcaattctt actccaataa
    14641 ttgccatcat ccattctcct tttctaactt ttttctattg gttctttgac aaaatctata
    14701 acttcactga atctaccttc tgtttcaaag ataacgattt cattttgtcc ttttctaaat
    14761 aaaccctcgg gaacaaacag tgaaagaatt ggtcctactt cccaaaaacg accaatattc
    14821 gtgccattca cccaaacgat ccctttccca aactcactaa ggtccaaata agtatcaccg
    14881 atttctgaca gttccgcttc aaagcggtaa aatgctggtt gacctgcttc ccattttttt
    14941 gaaaaatcga tgtgttctgt agtctctaaa ggtaaacaat actgtgtcca ttctgtgata
    15001 aaatgaagat cgctcatcac tcctgtacgt attccttttc tttgcgtatc agctagcagc
    15061 ttgtgtccat agttgacccg tcccatattt tcaaataata gatcaatttg atggtcttct
    15121 ttctcaagtg tcaatggaat ggtctcaccg atctctgttt ggtattgaat cgcttgcttt
    15181 tcttcatcga gaaaaacatg gacccgatca cgtccgtcga tgatccgtaa tttttcattg
    15241 accgcatctt ttttgatcgt tgttcgatac aataggtacc cagtattctg acccaattct
    15301 tccattgttt gaggataggt cgagtggatc ggcttgctga tggtatccag tgttgcgaaa
    15361 aagctgactt tttctttcaa gttgattcct tttttgacga ttgtttcttt tatccatggt
    15421 tccatttgct gcatttctgg aaaattttct gccatcattc gtttcagagc aaaatatttt
    15481 tccgttggat ttccctgctc atccaatggt gcatcgtaat cataggaagt gacttgcggc
    15541 aaatcaatcg cgcctctggc tgaacagcca ttcataaacc caaaattggt tccgccatgg
    15601 aacatataga gattgatact gccgatctgt aatgcttctt tgactgcatc cgctaattct
    15661 tccggatctc ttttaatgat cggctctttc caacgattaa accagccatc ccaaaattcc
    15721 atacacatca acggccattt tttaccatgt gcttcaaaaa actgggccat atctgcaaaa
    15781 ttttcttttg cttttgagcc aaaattccct gtcgtaaaaa catcatcgtc aatcaaaatg
    15841 cctgctctta atgtggcttg ccaaggtcca tcagaagtga atagcggtac atcgattcca
    15901 ccttcctcca agtatttttt tacttggcgc aggtatgctt tctcttcacc atacgaaccg
    15961 tactcatttt ctacctgcac cattaagacg ttgccaccat gagtgactag atgaggaacg
    16021 attttaggta gtagcgtttg gtaataccgt ttgacatact tcatgaagcg aggatccgct
    16081 gaacgaatac ggcactcctt tgtcaatagc caagcaggca agccaccaaa ttcccattct
    16141 gcacaaatat aaggggctgg acgtacgatt gcgtataatc caagggattg cgctaactct
    16201 agaaaatggg aaagatccaa tattttcgaa aaatcatagg ttccttcttc tttttcatgg
    16261 agattccaag gcacataggt ttccacagta ttaaatccaa gcgctttcaa attgtgcaat
    16321 gaccgctccc aattcgtcgg atgcactcgg aaataatgga tggcaccaga taaaattttg
    16381 aaaggttgac cgtctaacaa aaactcttct tttatttcaa atctagtcaa gcaaatttcc
    16441 tcctcgtttt tagaaaccgc tttctaattt tataatcaac gaatctttct gacgttgatt
    16501 tcccccctaa aataatcgac tttcacagga tcgatccatc ctgttttttc ttgttgtgca
    16561 ttcgccatcc cacaggccat cgcaaacgca agtgtttctt caatcgtata gctctttgat
    16621 agagcatgtg ctaagcctgc aatagtcgaa tctcctgacc ccacaggatt caccaccgcg
    16681 attttaggaa tcgtaacctg atagaagtgt ctccctactt tagcgattgc tccttttgcg
    16741 cctagcgaaa gaacgatcca attgattcct tcaaaaagcg gatgatccaa taatgattgg
    16801 atttcatcta acgaagtagc cgaagaaatt gtttggtgga ccaactctcc taattcttcc
    16861 aaattaggtt tgatcaaaaa tggtttttcc ttgccttcca agacttcaag caaggtttga
    16921 ccagaagtat ctaacaatac tcgtgttcct tttttattcg ttagggcaag taactctgca
    16981 taataacttt tagaaaatcc ttgaggcaga ctacccgaaa tcgtaaagat atcagttgtt
    17041 tccattagtc gtgcaaaatg ctcggtaaat ttatgttgct cttcttccgt cacaacagga
    17101 ccgctttcca atagttctgt ttgattcact ccatgtaaaa tagcaatcga atctctcgtt
    17161 tcccctttga taggtaaaaa ttgatgcgtt atccctgatt gatcgagttg ttctttgata
    17221 tatgctccat gctgcccgcc gactaatcca gtagctaaaa catcatctgc taataaatga
    17281 aggacacgag caacattcaa gccttttcct ccagccgttt tgttgaccgt tttgacccga
    17341 ttgacttggt cgatccgtaa ttcatccaac gtgtaagcga tgtcaattga aggattcatg
    17401 gtaactgtcg tgatcatttt gctgtcaccg aatcttcttt tactttgtca aaccaagggg
    17461 tggcgttttc atttatcact ttattcaacg attcgatatt ttgtcgccct tgtgttctca
    17521 accactcaat cgcaccttct ctaccatttt caacatatgc tttgactcca tctttccaag
    17581 tcgcacgtcc gcataacaca ccgttgaatg ttgaaccaga ttcatgagca aagatcaatg
    17641 tttgttggaa caatgcagtc gaaacccctg cactcaagaa gataaatggt aacggtgttg
    17701 catcactttg ttccttgaag aatgccgctg cttctttttt actatacgca atttcaccat
    17761 cagaataacc ttctacatag ttcatattga ctggcacttc tactttcaat acatccactt
    17821 tatatttagg atcagaaaat tctttcatca tctcgatcac tttatgcggt ttgatcttcg
    17881 catattctaa tgaagtgctg tcattggttt tggcatcata cgaaaccaat tctagataaa
    17941 acggacgctt ttcacctgca cattcactgc ctaaacgttc gataaataca tgtttcaaat
    18001 gattgatctc tgggtcttca tctacatcat aataaagcaa aaacttgatc gcatcggctg
    18061 tttgttcctt taatctaagc gtagaccaat ccgctaataa atcaggcaga cgtcctggag
    18121 ttgtcgcatc ataacccgtt ttttcatagg ctaaaagtag tcctgcttcc tgatgtctgg
    18181 ctttagcagc tggcaatcca tactctggat ctagtaaaat agcagaagca taaggcgtta
    18241 attctgctga tactaattct ttgaattcag tgatctgttg cgccgttggt tcttttccta
    18301 acgctttgat catctttttc aaagcaccgc gttgatcaat tgccaatgca ctgatgattc
    18361 cttcttttgt tgataaactc ttcatagctg caattttttc ttcattcatc cttcgtttcc
    18421 cctttgttca catattcatg gataatgaca cctttgacca ctcgattgac tgtacctgta
    18481 ggcgatggtg tatctggttt attcgctact tttatcgacg tatcgagcgc aatgatttgg
    18541 gctgtcatga tcataggtaa ggcaagatac gcatcaggta ggctgatcga agcttctaac
    18601 aaaagacttg tacctgtgaa atcgatgttt cctggttggg cgatcgcgat gacactcgtg
    18661 gtaatttggt catcttttat ttcttctaga atatctaagt cataatttct tgtatacggt
    18721 tggttattaa caaaaccaat aacaagtgtt tcgctattta aaaacgattt tggtccatgg
    18781 cggaagccca tagaagaatc aaagatcgtt gccactttac cggctgtcaa ttccaatatt
    18841 ttcaattgtg cttctctcgt tgcaccagcc aaacttcctg aaccaagata ggcgatacgt
    18901 tcaaatgact taggcaataa tttttggatt tcttgatctc gtgaaattag atcttctcca
    18961 gttctgatca attgttgaag ataactttct tttttctcaa cggcagtttg gtcgaatacc
    19021 cataacgcac tcaacaacat gcacgtaaaa cttcctgtca tggcaaatcc tgcatcattt
    19081 gatttttctg gcatcagtaa aagataattt cgttcatctg tcgctgcatt ctttgctaac
    19141 tgaccttctt tagcacaagt aatcgtcaaa tgaaacaatg tcttgatttg ttcattggca
    19201 atcttgactg ttgccacact ttctggacta tttccactac gtgcaaatga caccagtaat
    19261 gtcggttcat ccggaaatag aaattctttt ggtgaagaca cgatatctgt cgtaccaaag
    19321 ctatgaaaaa taaagtgatc gttatcaccg tattcattta aataaggtac tgctgtatcc
    19381 ccgacatatt gtgatgtgcc tgcaccagta aaaatcactc gaactctttt gccatttgct
    19441 gcttttttta tctggtcaaa aaacaatgct aatttttctt gattctgtgc taatatctca
    19501 gctgtttctt tccataattg cggttgctgt ttgatttctc gcgtagttat ttccgcaccc
    19561 aactctttta atttatcctt cgatagttca aacatgttgt tttccctctc aatcattttc
    19621 ggacgtggcg gatcttataa tgaaactggt ctgctctcgc tacactatcc gtatattcaa
    19681 taatttcatt tttcacatta taggtcgttc ggcttagata taataccggg gcaccatcag
    19741 cgatacgaag cggatcagca tctttctttt gaacgatact tgcataaaat tcttcatctg
    19801 ctaattggac ttgctgttca taatcctgag aaaacacatc atacaatggc ttactttcaa
    19861 gtaatggcag atcaagacca ataaatagtt ttgcaggtaa ataagaacgt tccaccatca
    19921 atggttcgtc atccgctaaa cgaagtcttt tcaatttgaa aactgtttcg cctaaattga
    19981 tgtttagatt cttggcaacc tttttgtttg cttccatttg cttgaattcc aagatgactg
    20041 tattaggcac ccgtcccaat tccctcatct cttctgtaaa actatacgct cccgccagat
    20101 cagacgactt atctgataga tcagacacat atgttccttt tccgtgtcgg cgataaatat
    20161 acccttgcgt ctctaattct tgcaaagcaa ggcgaaccgt tgtccgactc actccataat
    20221 gctgacaaag ttctctttca gataacaatt tgtcatgagg tgtcatctgt gtttcgatac
    20281 gttctttcaa tacgtcaact agttgataat ataatgattt tcctttactt gagctctcca
    20341 taaaaatacc tcttctttac tggttataac cacaacacaa gaatacccac atattcctca
    20401 tttgtcaaag ggtttttggc gtaaaaaagc tattataaca aggtttcaag gtaatttaga
    20461 actggtatca aaagatacct caaaaatacc agttgacaaa taaaaaacaa aaaaaagctg
    20521 aatcttggca aagattcagc taaatggtct agagaagtaa agaaaaaata ttctttatac
    20581 atctgtggct atttctacta tcgacaagca aatgggccta atagactact atgtaaataa
    20641 aaatggcttt ctctaaataa agtaggattt tttatttttt agataacttt tctctcatgc
    20701 cggtctgata gtcgaaattt tgttgtacat ttttaaaaat aacaaaaatt ttttatgata
    20761 attctgtctt tttctagttt ccttatggcg taagtttaac gaataagaat ttagtatcta
    20821 tacttttata ttacaaaaaa tattcgtaaa attcgggctc tatacttttc gatgtttgtg
    20881 aacttta
//
LOCUS       DAZAFF010000047        20756 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000047 DAZAFF010000000
VERSION     DAZAFF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.047"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..366
                     /locus_tag="ACG9BB_001625"
     CDS             49..366
                     /locus_tag="ACG9BB_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFN2837569.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(457..822)
                     /locus_tag="ACG9BB_001626"
     CDS             complement(457..822)
                     /locus_tag="ACG9BB_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837570.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1030..1512
                     /locus_tag="ACG9BB_001627"
     CDS             1030..1512
                     /locus_tag="ACG9BB_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837571.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1709..2599
                     /locus_tag="ACG9BB_001628"
     CDS             1709..2599
                     /locus_tag="ACG9BB_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFN2837572.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2925..5399
                     /locus_tag="ACG9BB_001629"
     CDS             2925..5399
                     /locus_tag="ACG9BB_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN2837573.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5502..7238)
                     /locus_tag="ACG9BB_001630"
     CDS             complement(5502..7238)
                     /locus_tag="ACG9BB_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN2837574.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7235..7939)
                     /locus_tag="ACG9BB_001631"
     CDS             complement(7235..7939)
                     /locus_tag="ACG9BB_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN2837575.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8070..8573
                     /locus_tag="ACG9BB_001632"
     CDS             8070..8573
                     /locus_tag="ACG9BB_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFN2837576.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8533..8871
                     /locus_tag="ACG9BB_001633"
     CDS             8533..8871
                     /locus_tag="ACG9BB_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFN2837577.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8892..10310
                     /gene="ffh"
                     /locus_tag="ACG9BB_001634"
     CDS             8892..10310
                     /gene="ffh"
                     /locus_tag="ACG9BB_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFN2837578.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10422..11000
                     /locus_tag="ACG9BB_001635"
     CDS             10422..11000
                     /locus_tag="ACG9BB_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFN2837579.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11004..11525
                     /locus_tag="ACG9BB_001636"
     CDS             11004..11525
                     /locus_tag="ACG9BB_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFN2837580.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11604..12158)
                     /locus_tag="ACG9BB_001637"
     CDS             complement(11604..12158)
                     /locus_tag="ACG9BB_001637"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFN2837581.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12411..12686
                     /gene="rpsP"
                     /locus_tag="ACG9BB_001638"
     CDS             12411..12686
                     /gene="rpsP"
                     /locus_tag="ACG9BB_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFN2837582.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12698..12949
                     /locus_tag="ACG9BB_001639"
     CDS             12698..12949
                     /locus_tag="ACG9BB_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFN2837583.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13073..13864
                     /locus_tag="ACG9BB_001640"
     CDS             13073..13864
                     /locus_tag="ACG9BB_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837584.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14034..14555
                     /gene="rimM"
                     /locus_tag="ACG9BB_001641"
     CDS             14034..14555
                     /gene="rimM"
                     /locus_tag="ACG9BB_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFN2837585.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14545..15306
                     /gene="trmD"
                     /locus_tag="ACG9BB_001642"
     CDS             14545..15306
                     /gene="trmD"
                     /locus_tag="ACG9BB_001642"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFN2837586.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15442..15789
                     /gene="rplS"
                     /locus_tag="ACG9BB_001643"
     CDS             15442..15789
                     /gene="rplS"
                     /locus_tag="ACG9BB_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFN2837587.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16099..16341
                     /locus_tag="ACG9BB_001644"
     CDS             16099..16341
                     /locus_tag="ACG9BB_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFN2837588.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(16429..16575)
                     /locus_tag="ACG9BB_001645"
                     /pseudo
     CDS             complement(16429..16575)
                     /locus_tag="ACG9BB_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            16632..16946
                     /locus_tag="ACG9BB_001646"
                     /pseudo
     CDS             16632..16946
                     /locus_tag="ACG9BB_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            16975..18234
                     /locus_tag="ACG9BB_001647"
     CDS             16975..18234
                     /locus_tag="ACG9BB_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2837589.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18287..19183
                     /locus_tag="ACG9BB_001648"
     CDS             18287..19183
                     /locus_tag="ACG9BB_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2837590.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            19194..20063
                     /locus_tag="ACG9BB_001649"
     CDS             19194..20063
                     /locus_tag="ACG9BB_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2837591.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIELFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            20073..20748
                     /locus_tag="ACG9BB_001650"
                     /pseudo
     CDS             20073..20748
                     /locus_tag="ACG9BB_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
BASE COUNT         6842 a         3576 c         4261 g         6077 t
ORIGIN      
        1 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
       61 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      121 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      181 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
      241 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      301 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      361 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      421 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      481 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      541 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      601 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      661 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      721 cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa
      781 agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac
      841 aagtgttatt attttgttag ttttattgta aattgaatcc tgcaataatc aattactttt
      901 tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt
      961 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
     1021 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
     1081 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
     1141 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
     1201 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
     1261 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
     1321 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
     1381 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
     1441 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
     1501 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
     1561 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
     1621 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
     1681 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
     1741 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
     1801 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
     1861 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
     1921 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     1981 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     2041 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     2101 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     2161 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     2221 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     2281 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     2341 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     2401 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     2461 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     2521 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     2581 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     2641 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     2701 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     2761 aaaaaagagc tacgacatag aactgtcgta gctttttttt tgctatatga caggaatgaa
     2821 aggtgtttgc aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag
     2881 aagtttggaa atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga
     2941 taaatcgaag taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt
     3001 ttccatcgta taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg
     3061 cagcaaataa ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca
     3121 tatttacgta tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat
     3181 cctataagaa cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc
     3241 aggaaattcg atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca
     3301 atgtcatacg agacgggata gaacaaacaa tagatataga agaccttgta atagatgaca
     3361 ttgttatttt agagactgga catcaaattc cagcagatgc agaggtcgtg gaagggaaac
     3421 tgcaagttaa tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg
     3481 atccattaat gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag
     3541 ttggagcaga ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag
     3601 aacagtcgga gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta
     3661 ttcctatcgg tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag
     3721 aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc
     3781 tgacgacgat tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac
     3841 acaatatgaa aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag
     3901 gaacgatcac agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa
     3961 taatcagtca gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag
     4021 gagacaatgc aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct
     4081 atcaccatgt tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca
     4141 aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg
     4201 aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg
     4261 ggattcttga agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg
     4321 ctaatcctat cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag
     4381 ccatcaaagt gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag
     4441 ggatttcaca tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag
     4501 cagctgtcga aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg
     4561 ttcaattgct aaagcgtcgt aacaatacag tagctatgac aggtgatggc gtgaatgata
     4621 ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca
     4681 tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg
     4741 cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga
     4801 atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag
     4861 aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg
     4921 ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag
     4981 ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga
     5041 ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag
     5101 ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc
     5161 ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca
     5221 ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga
     5281 tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc
     5341 aaaagccgtt tagtcgcgta gttaaaaaat ttctatcgat atttaaacga gaaaattgac
     5401 gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga
     5461 tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta
     5521 acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc atgaaggagt
     5581 tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg ccggcttcgc
     5641 gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct
     5701 ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc
     5761 tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa
     5821 aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt
     5881 ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa
     5941 attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc
     6001 agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc
     6061 gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga
     6121 gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc
     6181 actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag
     6241 tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc
     6301 agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact
     6361 tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga
     6421 ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg
     6481 ttcagtaacg tatataattg ctcttctgtg gtagtatacg cccgatacat cttactcatt
     6541 tgttcgctca acgcattgat cgtctgatac aattcttccc attgatcggg tgtttgcatg
     6601 attacttccg cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc
     6661 aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa
     6721 gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt
     6781 gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg
     6841 acatataaaa gatccccatt caaagtagta cttttgcgca aagcgctacc taggttccca
     6901 ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg
     6961 tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga
     7021 tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat
     7081 tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc
     7141 tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata
     7201 agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat
     7261 agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt
     7321 ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc
     7381 aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa
     7441 caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat
     7501 aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt
     7561 ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc
     7621 gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat
     7681 ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca
     7741 gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat
     7801 caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct
     7861 tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa
     7921 caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta
     7981 gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca
     8041 ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc
     8101 tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa
     8161 ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc
     8221 ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga
     8281 cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa
     8341 aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa
     8401 cccattcgat ttatgacggg agtttttccg gaataaaaaa aaatggtgcc ggagtggcta
     8461 ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta
     8521 gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat
     8581 tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt
     8641 tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc
     8701 aagcggacaa gcaagatctt ggaggattat gaaaaaaaac ttcatctttt ttctaattat
     8761 attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa
     8821 gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga
     8881 atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa
     8941 taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc
     9001 ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac
     9061 gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa
     9121 aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat
     9181 caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct
     9241 ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg
     9301 caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag
     9361 acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga
     9421 tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc
     9481 atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg
     9541 agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct
     9601 tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag
     9661 gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg
     9721 gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg
     9781 gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactatgac gagaaaaaag
     9841 cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac
     9901 agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa
     9961 tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa
    10021 aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa
    10081 gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga
    10141 tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca
    10201 tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga
    10261 tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa
    10321 acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag caatttttat
    10381 tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg
    10441 aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg
    10501 accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga
    10561 cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat attaaaaaag
    10621 tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta
    10681 accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag
    10741 aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt
    10801 cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag
    10861 atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag
    10921 ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta
    10981 tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag
    11041 atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta
    11101 agcaaaccac ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa
    11161 cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt
    11221 tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc
    11281 ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg
    11341 gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga
    11401 ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc
    11461 gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa
    11521 agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt acatagttgc
    11581 cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca
    11641 tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt
    11701 agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag
    11761 acaagtgatt gtcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca
    11821 gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca
    11881 tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg
    11941 acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct
    12001 ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg
    12061 tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt
    12121 cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact
    12181 atctataaca atcttacaaa aaataagtaa ctggagcaaa taaaaacact tactgacaaa
    12241 agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt
    12301 cctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa
    12361 aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta
    12421 aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg
    12481 attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga
    12541 aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg
    12601 cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc
    12661 acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt
    12721 cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga
    12781 gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat
    12841 tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa
    12901 tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct
    12961 gtactcgaat tttcgagtgc agcttttttg tttttttatt aattgaagaa atgattattt
    13021 ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa
    13081 gaaaatgtta tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga
    13141 atcagacaat acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag
    13201 tagtaccaaa acggctacca ccactgaaca aacaatggct acctcaaata atgagaaaaa
    13261 gactgcttta gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc
    13321 agtctcttca ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt
    13381 atattacaga acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc
    13441 tattgcaacc tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt
    13501 gcagcccttt gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg
    13561 gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat
    13621 tcgaatccgt gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt
    13681 agcttatttg gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga
    13741 tatgggtgat acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta
    13801 tacgatcact catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt
    13861 gtgaaatcag cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa
    13921 aaatagcacg ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt
    13981 gcctcttcac acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg
    14041 aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta
    14101 tctcaacaac ggatttccca gaagaacgtt acaaaaaagg aacggtgtta acactattcc
    14161 aagacggaaa agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg
    14221 acttattgag ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca
    14281 tattaaaagt gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg
    14341 agatcatcgg tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct
    14401 tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc
    14461 ttccatatat cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag
    14521 aaattccaga aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg
    14581 tttgaaggtc ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat
    14641 atcaatatct ctaacttccg cgattattca gataacaaac atcagacagt agatgattat
    14701 ccatacggcg gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa
    14761 gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt
    14821 aaacgattcg atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt
    14881 tgtggtcatt atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc
    14941 ttaggagatt atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca
    15001 gttcggcttt tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca
    15061 accggtttat tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta
    15121 ccagaagtat tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca
    15181 ctaagaagga cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa
    15241 atgacgaaat ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc
    15301 aagtgacttt ttctaaaata ttctttacag taggttgata atatgttagt attttatggt
    15361 gagtgcaaaa gcacttaact attacgatat tccgctatga gaaaagctca taagaatatt
    15421 tggaataagg agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta
    15481 cgttctgaca tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt
    15541 gaaggtactc gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct
    15601 ggaatcagcg aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca
    15661 ttcccattgc atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt
    15721 cgtgcaaaat tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc
    15781 cgtcgttaat cattgttatc agactgagaa agaacccttg gtattcaagg gttctttttt
    15841 cttagcatgt aatttgaaca ccctgatcca gtttttttct ttaattattg gtaggggaga
    15901 ataatggatt agaataaatc tacctataag tagtagtaaa aaaaagagca ctatgataat
    15961 atctttctta agagtaaccg ctttaataaa aaatccagaa tattctaaca taaatggagt
    16021 attcttagaa tcaaaaggag aaagaagatg gtttatactt atacggagca agaaaaaatg
    16081 aaagtttgag acacattcat gaatgatgga aaagtaaaac aagtaccaag ttcaacaaaa
    16141 aagaaaaaca tcctattgaa agaagtcttg aaacgctttg accatggagt gacttataca
    16201 gaaacagagg ttaattcgat actgctgaac gtattttcta gtggcgatta tgtcgaacaa
    16261 agacgttatc taatcacatt tggctttttc aaaagaagtt cagacggtcg ggcatatcag
    16321 atgatgggaa ttgaaaatta gaagttttaa tttatactca ttgaaaaaag ttcaagtatg
    16381 cgatattggt tctacaccgc atacttgaac tttttttgtc atctgtatct ataatacaaa
    16441 gccaagaacg ttattcaaaa tatggatagc tatgataaaa gctaatgagc gattcgcagt
    16501 taaataagca atcgtcagta aaatcgttcc aggagcgtac atgaaaaaat caattggatg
    16561 cgttacatga ataaagataa ttctctttta tttgtacgaa taaataccaa aacgtacctc
    16621 ctaaaattag attcaaagaa gatagcaaag aggaaaaaga tagttctgca gcagctatag
    16681 cggcatgtgg attgctagaa ctttccaaac aactgcctgt ttcagatcct cgtcatttcg
    16741 attacgaaga gatagcaatc aaaatcttgg aagaattaca gaaaaactat acaacgaaac
    16801 gagttaacgg atcaaacggt ctgcttctcc atgccgttta tgataaaaac agcttaaaag
    16861 gtgtagatga atgtgtgatc tggggcgatt acttttatgt agaaggaatc actcgtctag
    16921 ctaaaacatg gtactgttac tggtgatgta taagagaaga ggaggattca tataatgaag
    16981 cgatggaaaa aaatgatgat tggatcaata gttttaggaa tgtcactcgt atttggtgct
    17041 tgtggtaatc aagaagacag tagtgaagaa tcagcaaatg aaattaccgt ttcgacatgg
    17101 aattatgaaa caacacctga attcaaagcg ctctttgaag cattcgaaaa gaaaacggga
    17161 atcaaagtga aagcagtaga tattgcttca gatgattatg atacgaaact caccacgatg
    17221 ctttcttctg gagacacgac agatgtattg acgatgaaaa atcttttgtc gtattcaaat
    17281 tatgctttac gagatcagtt agtcgatcaa acagaacgaa ttagagaatt agacactgat
    17341 gctgctgaag aaacatatga aatgtatgat atcgatggaa aaacatatgc attgccatat
    17401 cgaacggact tttgggtact atattacaat aagaaaatgt ttgatgatgc aggaatcgaa
    17461 tatccaaaaa atctaacttg ggacgaatat gaagatttag caaaaaaact tagtaaaaat
    17521 gacggacaag tttacggagc atatcaacat atctggcgtt cgacaatcca agctattgca
    17581 gctgctcaaa acgaggcgaa tcttgttgaa ccagattatc aatatatgac tgattattat
    17641 gatcgagcat tgaggatgca aaaagaaggc gcgcagatgg attttggcac ggctaaatca
    17701 acgaaagtaa cctatcagtc tcaatttgaa gaacaaaaag cagcaatgat gtacatggga
    17761 acttggtata tggctggcat actcgcaaac aaagaagcaa gtaagacaga agtagaatgg
    17821 ggaattacag ctattcctca gaagaaaaaa ggagaatcgg tcacgacgtt tggctcacca
    17881 acagcatttg ctgtcaataa aaactcaaag aaacaaaaag cggcgcaaga atttattgaa
    17941 tttgcagcca gtgaagaagg agcaaaagta ttagcaggag taggagtagt tccttcatat
    18001 cgtacggatg agattgatca gctttatttt aatctcgctg gaatgccaac ggatgaaatt
    18061 tctaaaaaag ctttttctcc tgatgaaatc aaacttgaat ttccgattga tacgtatggg
    18121 cctgcaatcg ataagatttt acaagaagaa tatgacttag ttttggttgg agatgaaacg
    18181 ccggaaaaag gaacggtaaa tatggagaaa cgtgtagctg cggaaaaaga ataatcatga
    18241 aaggagagaa gccaagagat aaaacactct tggtttcgtc atcgaaatgg agaatagtat
    18301 aaataagaaa gctacaaatc atttgcgtag aaaacgaacg tggatcgctt attcgtttat
    18361 cgccccgaat ttcattggtt ttttcgtttt tacattaata ccaatcctgt tttcgcttgt
    18421 actagcattt atgaaatggg attctttttc aacgccagaa tttgctggac tagataactt
    18481 tcgtaaaatg gccaacgatg agacattttg gatttctttg aaaaatactt ttttatatac
    18541 cgctgctgta gtgcctggca cacttgtttg ttcgttaggg ctagctttac tactaaatca
    18601 gaaaattaga gggatgaagc tttttcgtac gatatttttc tttccgtatg ttacttcctt
    18661 agtagcaatt gctgttgttt ggaatatgtt gtttcatcca acagtgggac ctattaatat
    18721 ctttttaaga cgatttattg aacatcctcc aggttggact tcaagttcaa attgggcgct
    18781 ttgggcaatt gtaattgtca gtatttggcg agggatgggt tattacatga ttctatacct
    18841 tgcaggacta caaagtatcc aaaaagaact atatgaagcg tcttcgctag atggagcaaa
    18901 ccgttggaaa caatttttga atgtgacatt accttcgttg agaccaacaa ccttctttgt
    18961 aacgatcatg cttgtcatca attgtttcaa ggtgtttgac cttgtgcaag tgatgacgga
    19021 tggcggccct ggtcgagcga caaatgtgct ggtttaccaa atctacaatg aagcattcaa
    19081 aaaatttgat tttggttatg cttcagctat ttcacttgtt ttgttcgtaa ttgttttggg
    19141 cgttacggtt gttcaatttt cttggaataa gcgtcgtact taggaggtgt gatatggaag
    19201 agagtgttcg aaatgaaaga aaaaaagcaa aaattcgttc aaaaaacatg ccctttattc
    19261 aatggttggg tatgattctg ctgattgtat tagctttatt gacgatcatt ccttttttat
    19321 ggatggtttc tgcttcattc aaagcaaata atgaagtgtt aacgattccg attcaatgga
    19381 tccctaaaac ttggcattta gagaattatc tggttatttg gtctcgtatt cctttagtga
    19441 cattttttaa gaatacagct tttttaagcg ttgtgatcac gttgattcag ttgtttactt
    19501 ctagttttgc tgtttacggg ttttctgaac taaattttaa agggcgcgat attttatttt
    19561 tagcatatat cggcacgatt gctgtccctt ggcagtctta tatgatcccg cagttcatta
    19621 tgatgagaaa agtgggttta acagatacgt tgtggtcgtt ggtgctatta caagcgttca
    19681 atgcttttgg tgtgttcttg ttgaagcaat attacagtag tatcccagat tctttatgtg
    19741 agagtgcgcg gatcgatggc ttatcagagt gggaaatcta tcgcaagatc attttacctc
    19801 ttacaaaacc ggcgattgcc agtttgacga tcatcacatt cgttaataca tggaacgatt
    19861 atatggggcc attcatttat ttgtcttcta cggaaaataa aacaatccag ttagggttga
    19921 aaatgtttat tgagctattt gatgcagagt acgcattaat catggctgcc tcagttgttt
    19981 ctgttcttcc tgttgctttt atatttttag cgatgcaaaa atattttgta gaagggatcg
    20041 cttcatcagg aatgaaggaa taggaggtga ttatgaaaga gcagcgtttt gacaaaaatg
    20101 tttatatgga attcttttat tggttgtatc aatcattggc aagtagcctt tgtttttggt
    20161 gtgtcaatat cttgtatata gcagctgcgt tccttttgtc gatagatgga aggaatgtct
    20221 tttcctttat tttttcttta ctttttattg gtccaggttg catcgtgatg ataggtatga
    20281 cagaagaatt agcaaaagaa aaaagttttt caccagtacg tttgttcata aagacgtttc
    20341 gccaattttg gctaaaggga tttttttatt ggctatttgc ttggatagtt agtgtgataa
    20401 tgatttttga ttgtttcttt tttatacgtt tttcttatgg aaaatggttg attccgttgt
    20461 ttgttttact tgcttgtttt acttgcttgt ttatcagttt cctttagcat caactgctgg
    20521 tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat agcgttctac
    20581 tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac aagtttgttt
    20641 ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat cgtatttggt
    20701 ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac tttata
//
LOCUS       DAZAFF010000048        20063 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000048 DAZAFF010000000
VERSION     DAZAFF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.048"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..715
                     /locus_tag="ACG9BB_001651"
     CDS             56..715
                     /locus_tag="ACG9BB_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN2837592.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            800..1705
                     /locus_tag="ACG9BB_001652"
     CDS             800..1705
                     /locus_tag="ACG9BB_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN2837593.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1707..2339)
                     /locus_tag="ACG9BB_001653"
     CDS             complement(1707..2339)
                     /locus_tag="ACG9BB_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN2837594.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    2490..2615
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2659..3183
                     /gene="infC"
                     /locus_tag="ACG9BB_001654"
     CDS             2659..3183
                     /gene="infC"
                     /locus_tag="ACG9BB_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFN2837595.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            3255..3455
                     /gene="rpmI"
                     /locus_tag="ACG9BB_001655"
     CDS             3255..3455
                     /gene="rpmI"
                     /locus_tag="ACG9BB_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFN2837596.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            3508..3867
                     /gene="rplT"
                     /locus_tag="ACG9BB_001656"
     CDS             3508..3867
                     /gene="rplT"
                     /locus_tag="ACG9BB_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFN2837597.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            4119..5456
                     /locus_tag="ACG9BB_001657"
     CDS             4119..5456
                     /locus_tag="ACG9BB_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFN2837598.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            5504..7633
                     /locus_tag="ACG9BB_001658"
     CDS             5504..7633
                     /locus_tag="ACG9BB_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFN2837599.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            7608..8297
                     /locus_tag="ACG9BB_001659"
     CDS             7608..8297
                     /locus_tag="ACG9BB_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837600.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(8705..9382)
                     /locus_tag="ACG9BB_001660"
     CDS             complement(8705..9382)
                     /locus_tag="ACG9BB_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2837601.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(9518..9712)
                     /locus_tag="ACG9BB_001661"
     CDS             complement(9518..9712)
                     /locus_tag="ACG9BB_001661"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837602.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(9762..10202)
                     /locus_tag="ACG9BB_001662"
     CDS             complement(9762..10202)
                     /locus_tag="ACG9BB_001662"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFN2837603.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(10206..11051)
                     /locus_tag="ACG9BB_001663"
     CDS             complement(10206..11051)
                     /locus_tag="ACG9BB_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFN2837604.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11200..12618
                     /gene="pepV"
                     /locus_tag="ACG9BB_001664"
     CDS             11200..12618
                     /gene="pepV"
                     /locus_tag="ACG9BB_001664"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFN2837605.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(12675..13622)
                     /locus_tag="ACG9BB_001665"
     CDS             complement(12675..13622)
                     /locus_tag="ACG9BB_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN2837606.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(13845..14195)
                     /locus_tag="ACG9BB_001666"
     CDS             complement(13845..14195)
                     /locus_tag="ACG9BB_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFN2837607.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            14395..15474
                     /gene="pepA"
                     /locus_tag="ACG9BB_001667"
     CDS             14395..15474
                     /gene="pepA"
                     /locus_tag="ACG9BB_001667"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFN2837608.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            15618..15938
                     /locus_tag="ACG9BB_001668"
     CDS             15618..15938
                     /locus_tag="ACG9BB_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFN2837609.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            15960..16424
                     /locus_tag="ACG9BB_001669"
     CDS             15960..16424
                     /locus_tag="ACG9BB_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN2837610.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            16629..17234
                     /gene="ytpR"
                     /locus_tag="ACG9BB_001670"
     CDS             16629..17234
                     /gene="ytpR"
                     /locus_tag="ACG9BB_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFN2837611.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(17273..18562)
                     /locus_tag="ACG9BB_001671"
     CDS             complement(17273..18562)
                     /locus_tag="ACG9BB_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFN2837612.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            18990..19469
                     /gene="rlmH"
                     /locus_tag="ACG9BB_001672"
     CDS             18990..19469
                     /gene="rlmH"
                     /locus_tag="ACG9BB_001672"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFN2837613.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            19672..19797
                     /locus_tag="ACG9BB_001673"
     CDS             19672..19797
                     /locus_tag="ACG9BB_001673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837614.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            19794..>20063
                     /locus_tag="ACG9BB_001674"
     CDS             19794..>20063
                     /locus_tag="ACG9BB_001674"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFN2837615.1"
                     /translation="MRHSNFLAFLFAPNETHNLSDEFFKMFLKRYIDSNDDTKAAINY
                     FDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGG"
BASE COUNT         6415 a         3649 c         4107 g         5892 t
ORIGIN      
        1 tttaaaaagg ttggagttaa acacaatatt tagtgatgaa gtgagctgga ggtaaatgag
       61 cgtgatcgtt ttagcaggta caattggtgc cggaaaatca agtttaacag aaatgatggc
      121 tgaacacttt gatagtcagg cattttatga atcgattgat gataatgaag tcttgccctt
      181 attttatgca aaccctgaac agtatgcttt tttgctgcaa atttattttt tgaataaacg
      241 ttttgcaagc atcaaacaag caatgaaaga tgacaataat gtattggacc gttcgattta
      301 tgaagattca ctgcttttcc atctaaatgc agatctagga agagcaacag aaacagaagt
      361 tcgtgtgtat gatgaactgc tggaaaacat gatggaagaa cttccctatg ctgcccataa
      421 aaaacatccg gatttattag ttcatattcg tgtatctttt gatacgatgt tggagcgtat
      481 cgaaaaacgt ggtcgttcgt atgaacaatt gtcctttgat ccaagtctat acgattatta
      541 caaagaattg aacagacgtt acgatcaatg gtatgaagaa tacaaagaaa gtccaaaaat
      601 ccaaatcgat ggagaccgct ataattttgt ggaagatcct caagcaaaag aagaagtctt
      661 gaaaatgatc gaagaaaaat tagcagagat tcgccaaacc aaagtagcca tttgatttac
      721 agttgctcaa ataaagggta aacctttatt tgagcgactt ttttctttat actggaaaca
      781 aatgtcagga ggatagagta tgcgggaaaa acactatttt aatcaagacc gtaatttttg
      841 gatcaatgta cagcaaaacg atgtgctagc tgttaaaaac ttaaaagaag aatatggcat
      901 tagtgacgag atgctgactt attctctaga taaaaatgaa cgggcgcgcg tagagtacga
      961 ttcttttgat gaagcgttac tgcttgtatc aaatgttccg caccagcaaa aagtcgataa
     1021 tcattatgaa actagtccta tcgcttttat tttaaaagaa gacggtttgt ttacatttac
     1081 cacgccgaat accgagtacg tgattcgact aattcgatcg cttttggaac gaatgccaga
     1141 catgagtgta tacagtttat tgtttcgtac attgtttttg atttctgatt ccttttttcc
     1201 tttaatcgag gaagtgaata gtgaacgtca gcggcttaat ctgaaattaa gagaaaaaac
     1261 aacgaacaag aatctgctgc aactttcaga tttagagatc ggtttggttt acttagttac
     1321 aggaacaaaa caaaatgtag tacttttaga acaaatcaag gcattggcta tttatcgcaa
     1381 gttgtcagaa aaggaaaaag aagagttgga cgatgcgctg atcgaagcac gacaagcagt
     1441 tgaaatgacg aacttagctt cgcagatttt agaccagctc tctggcactt ataataattt
     1501 gctgaataac aacttgaatg acacgatgaa atttttgacg gtctggtcgc tgatcttgac
     1561 cgttccaact attgtgacag gattcttcgg aatgaatctc cagttaccct ttacccattc
     1621 cgttttcgga tggggaatcg ccttgatcat cagtttggtc ttatctattt ggatgcttat
     1681 tgcactgtgg cggcggattc gttgatctag tctttgatct gcaaaccaag ggcttgggcg
     1741 atcacaagtg cttgctcttg gttgacgatt agtcccgcca aagaatcttg agataaagcg
     1801 attgctgtga agaaattagt cgtaaagtct aatcccttca gagaagttcg aaaccagttc
     1861 gaaccggtca actgattgtc agacaaaaaa aggtttttcc aagtgacctc ataaaattcg
     1921 ctgtcttcta attgacaatg atcgaaggag acagctttca agtttgtact actaaaggaa
     1981 gccatcgaag caatacaatc agtgaaagta cagtctcgaa gataactttc ggcaaaattc
     2041 gtacctgtta atttacattg atgaaacacg acttggtgga aactgccacc aatccactcc
     2101 agattggaaa aatcacattt ttcaaaaatg acattactgc attcaaaatg acgtaaaact
     2161 gttttttgca tcgtaagttt ttggatatga ctctctctta ataccatatt tttggcatcg
     2221 agatagcttt ggtcaccccc gttgatcaga agaccagaga agatctcctc atcttctaaa
     2281 taaaactctc cctttgtaag aagaggaaga gaaggtggaa taggttcgat ttttttcatg
     2341 tgggttcacc tcgtttttct ttagtatagc aaagaagcgg gaatcactgc tagaaagtct
     2401 gctaatctaa tcattttcag aaaaggaaga caaactgtat gaaaaacatt gacaaaaaga
     2461 ttagtttttg ctatactatg atggttgaga aataaaagca gaagcacccg cttctcgcct
     2521 tagttgataa tgtcactggg ctagatagtt ttcactggtt ctattaggtg aagtacgcgc
     2581 gggttctgta agagaactcg tttttttatt gccgtttatt cggtaagctt ttctttctct
     2641 cataatattt ggaggtgaat gaccatagca aaggatatga tggttaacga cggcattcgt
     2701 gcacgagagt tacgattgat cggttcagat ggtgagcaat taggagttaa gacaaaagca
     2761 gaagcattga atattgcaga acaagcaaac ttggatcttg tgttagttgc ccctggtgcg
     2821 aaaccaccag ttgcgcgaat catggactac ggtaaatacc gtttcgaaca acaaaagaaa
     2881 gaccgcgaag cgcgtaaaaa acaaaaagtg atcaatgtaa aagaagttcg cttaagtcct
     2941 acgatcgact tgaatgactt caatacaaaa cttcgtaatg cacgtaagtt cttggaaaag
     3001 ggcgataaag tgaaagcttc tatccgtttc aaaggccgtg ccattaccca taaagaaatt
     3061 ggtcaaaaag tcttgaatcg cttagctgaa gaaactgcgg atattgcaac agtggaacaa
     3121 aaagcgaaaa tggacggacg cagcatgttc ttgacgctgg caccgaaaaa cgacagtaag
     3181 taatttgaat gactaactgg ctgttggaca gccgatagtc ggataacgaa agattttgag
     3241 gaggaaatat agtcatgcca aaacaaaaaa cacaccgcgg atcagcaaaa cgtttcaaac
     3301 gtactggtaa aggcggattg aaacgcttcc gcgcgtttac aagtcaccgt ttccacggca
     3361 aaacaaaaaa acaacgccgt caattgcgta aagcaagcat ggtttctagt ggcgacttca
     3421 aacgtattcg ccaacaacta gcaaaaatga aataagaagt acaaataaca gaatctgaag
     3481 atttattttt attaggagga attatacatg gcacgtgtta aaggtggaac agtaactcgt
     3541 aaacgtcgta aaagagtgct aaaattagca aaaggttact acggttcaaa acacacttta
     3601 tttaaaacag caaaagaaca agtaatgaat tcatacaact acgcatatcg cgatcgtcgt
     3661 caaaagaaac gcgacttccg taaattgtgg attgctcgta tcaacgcagc agctcgtatg
     3721 aatggcttga gctactcaaa attaatgcac ggtttgaaag ttgctgaaat cgacatcaac
     3781 cgtaaaatgt tagctgactt ggctgttaac gatgcagcag catttactgc attggctgac
     3841 caagcaaaag aagctttggc taaataagct aaacaattat aaagaggaaa gaagataacg
     3901 aaaagttatg ttctttcctc tttttttatt tacaatcctc catcaaatga aacaggacaa
     3961 taacgtataa gtctaaaata gcataattaa ggttttttaa ttgaaaactg aacatttatg
     4021 gtcacccttt aatgaaagcg ctaactacat aagttagaat gagtttgact gatatattag
     4081 tttgcttatc agaaaaacaa aatggacgga ggaataacat gagtgatatt attattggta
     4141 ttttgttagt tatctcattc tttttcatgg tctggtactg tgtaaaaggc tacaacttga
     4201 tggtcggctt tgccatcatg gctactgtat ggatgggatt agcgctagtc ggaaacacgt
     4261 tttcaccaaa cccggcgatg gaagggcaag gagtaattga tgtgttgacg catatctata
     4321 cgacggggcc agctgagtac gccaaatcga tcttggtaaa tgtcttcttt ggtgcctttt
     4381 tcgggcgtgt tctagtagat agcggaatcg cagctaccct gattcgaaag gtagttgaac
     4441 ttggagggga caagccaaga atcacgatga gcttactatg tattgtaacc gctgttatat
     4501 tcatgtcgat gacaggaatc ggaccagtga tttctattgc agttattgtg ttgccaatct
     4561 tgatgtcatt aggtatttca gtaccggttg cactgttctc atttatgggc tcgattatgg
     4621 ctggaatctt tgctaatatc gtcaatttca aacaatatca aacgatttat gctggtttca
     4681 atccagcggc tgaaagctat acttataatg actatttcca aattggtatg attgggatga
     4741 ttgtaagctt agttgtggta ctaacggtcg ctaatatatc aatgaacaaa aagaaacgct
     4801 atgcaatggc agcaaacgta ccagctgaag gtggcgacgc accaatgatc tcatggttag
     4861 ccgtattatt accagtttta ggggtcgttc ttttagatct tccaatcatc ttaggtttta
     4921 ttctagcagg tatctgggca ttgctgttta ctggaaaatt gcgcggaggt tataaagaaa
     4981 tctgtcgtca gtttgcgaaa ttatttacag atggcgcagt agatgttgcg ccaatggttg
     5041 gtttcttgat gaccttagcg atgttcaata attcagctgc gtatgcttct ccttattttt
     5101 cagctatttt tggagattgg ataccgcaat caccattagt cttggcaatc gtatttgcta
     5161 tattgacacc actaggattt ttccgcggtc caatgaatct agtcggttca ggctcagcta
     5221 ttttagctgt cgtattagct gttaatccaa cgatgtcgcc agcctttttg ttcccgttat
     5281 ttgcaatcac aacgattgct cctcagcatt tagatattac acaatcttgg gttgcttggg
     5341 gattaggtta tacaaaagta acttctcgag aatatatgaa gaaatccatc ccaactggtt
     5401 ggatcatcgg agcgatctta tgcctgatca cgttcttatt gtacgggaac gcctagtaaa
     5461 caaaacgtcg ataaaaacga ttagatgaat ggaggaaaag aaaatgaaac gttttgtgcg
     5521 aatgggaatt gatgttggcg gtacacatac gaaagcagtc gcaatcgata atgcgacgca
     5581 cgaaatcatc ggaaaatctt cagtgaaaac aacccacgat gatgtccgtg gagtagccgc
     5641 aggtgtcgtt cagtcttttc aaaattgttt gctggaaaat aatatcagtc cagaagatgt
     5701 tgtgtttgtt gcacattcta ccacacaagc gactaatgca ctgattgaag gagacgtagc
     5761 aaaagtcggc gtaatcggga tggcaaaagg tggattagaa ggttttttag caaaacgaca
     5821 aactagattg aacgatatcg atttagggaa taagaaaaaa atagagattg tcaatgcatt
     5881 cttacctgtc aaacacttaa atgtagaccg tgtttcagaa acgatttctt ctttagaaag
     5941 agaaagagcg gaggtcttgg tttcttctat ggcatttgga gtagataatg gggaaccaga
     6001 acgagttgtt tatgaagcag catcggtaaa atcgatccct acaacgatgg cttctgacat
     6061 cacaaaatta tatggattga caagaagaac tcggacagca gcaatcaatg catctatttt
     6121 accaaaaatg ctagatacgg caacctctac agaagattcc gtacgagaag caggggtcaa
     6181 tgtttctttg atgattatgc gcggagacgg cggagtaatg gaaatcaatg aaatgaaaaa
     6241 acgacctgtt ttaaccatgc tttccggtcc agcagcatca gtcatgggct ctttgatgta
     6301 cttgagagcg tctaatggtg tttattttga agtaggcgga acaacaacca atatcggggt
     6361 cattaaaaat ggacgtccag caatcgatta ttcaattgta ggtggccacc caacatatat
     6421 ctcttcatta gatgttcggg ttctaggtgt ggcaggcggt tcgatggtac gggctaatca
     6481 gtcaggaatc atcgatgtcg gtccacgttc tgcacatatt gccggtctag attatgcagt
     6541 atttacggag acagaaaaaa tcaaaggtcc taaggttgaa tttttctctc ctaaagaagg
     6601 agaccctgct gattacgtca aggtagtgat ggaagacgga gaagaagtga ctattacaaa
     6661 tacatgtgca gccaatgtcc ttggattagt gcaagaagaa catttttctt atggaaatgt
     6721 cccatctgca cgaaaagcca tccaagcatt agctgattat tgtcatacga cggttgaaga
     6781 catcgcagaa cagatcatgg aaaaatccta tgctaagatt gaaccggtga ttttagaatt
     6841 ggcagacaag tatcacttgg agaaagatca gatttctctt gtaggtgtag ggggcggcgc
     6901 tgcctcattg attacttact ttagtaataa aatgggtgtg aaatattcga ttcctgaaaa
     6961 cgccgaagtg atttcttcta ttggtgtagc acttgcaatg gtccgagatg ttgtagaacg
     7021 tatcatccct tcgccaagta aagaagatat acgatcgttg aaaaatgaag cgatgaataa
     7081 agcgattgaa tcaggcgcta caccagaatc gatcgaagtc cacgtagaaa tcgatccaca
     7141 gacttcaaaa gtaacggcta tcgctactgg ttctacggaa gtgaaagcaa ccgatctaac
     7201 caaggagatc acaacagaag aagcactaga gcttgctgca gaggatatgc gattaaataa
     7261 aaatgaagtt tgcttattgg aaaatacacc tttcttttat gtatgcggcg aacagaatcg
     7321 ttcaaaaaat gctggaagtc ttcggatcat cgatcaaaaa ggatttatca aggtccaacg
     7381 tggacatgct tcatgtatga aaacaactgc agccaattat atgacagctg tcgaacaact
     7441 ttgggaagat atggcagttt atcaaactga attgattgcc cgaccagaat tttatctctg
     7501 cttaggagca agagtctctg attttacagc gacggatctg gaacaactac agcttttgat
     7561 ggatctggaa gtatctacta tggaaccgga ggaagaagtg atcgtcgttg ccggaaatat
     7621 caaacaaaca taagttagaa gagttactaa acgaattaca acagatcccc gaagaaattt
     7681 ggggattcta ccagtttcgg cgagatttat tttggaaaaa gattcctttg tccaagcaga
     7741 aaattcttat acaacaatca atcgattgtg gtatcgaaac ggcatgtagc atcaaaaaaa
     7801 agtacccttt tgccgatgta ggagaaattt gtgaacaaat ggctgttcca atcgtttcct
     7861 gtgaatcaga gcaaataaac gaacgaatca cttttgcaac ttatgcagaa gatgaaggga
     7921 ttcgattgat gacggagccg ttagaaaaat taaaatgttc aggactagct tcgatctcta
     7981 aagaaacagc gcaagctttg attattggac atgaactatt tcaccacatc gaagcttctg
     8041 ttaagggaat ttatacgcaa agcgaaaaaa ttgtgctgtg gcggttgcct ttttatacac
     8101 atcaatcaaa cattcgtgca ttgagcgaga tagcagcgat gagtttttca aaagagatga
     8161 atcaaagcga tttttcgcct tatgtattag aggcagtctt actttggcca tataatgaaa
     8221 ttcgtagcca agggatctta gaagaaataa aaaaaataga aaaaagatgt acggagtatg
     8281 atttagatta caaataaaga gactgtgaca aaagtcgtgt cacagtcctt tttttccgaa
     8341 taaacgatgt tctcctgtca actcctcggt cacaagtcaa aatattcaac aaacatgaga
     8401 agctgttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     8461 ggaaaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaagt taaaaacata
     8521 agaagctatt tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct
     8581 ctcaataaac ggtgtgcaat cagctgaccc tcggtattag ctcaaaaaaa acggcaaaat
     8641 atgaggaact attttgccgt ttctttctct aatactcagg tcaaaacgct gattccaaaa
     8701 cctcttattt gatgggtctg tcctgaatat cttcaaaata atcggggtaa ttatgaatca
     8761 cgtattcttg ctcctcgata atcatatgta aatgcatcgt gcaaagaaat ccgacttcat
     8821 caaagttatg atcaatcatt gattgaagca ttgtctgatg ttcatctaat actcgacccc
     8881 agtctaattc tgaaatcgtc aaacgtaacc atctgaaacg cgtactataa ctttctagcc
     8941 aatcccaaat ttctcgtttt ccagcaattt cataacaagt tcggtgaaaa tcagtatcca
     9001 aatcaaaaaa agctttttta ttttgagcga ttactgcctg atcggtttgt tctagtattt
     9061 tttctagttt agcttgatct tgcttggtca ttttcgcact gcattcttgc aggatcggtt
     9121 tttccaactt ttcccgcgta taacgggcta atgaagcaga tcggatatcg atttttgaaa
     9181 cataagtgcc ggactgtgga atggttttta caagctcttg attgcgcagt tggatcagtg
     9241 cttctcgaat cggtgtacgc ccgatagcta gttgttcttc taacacttta tcggagattt
     9301 tacttccagg tgctaactcg gaataaatga tttgtttacg tatagattca taagcttgct
     9361 gttgcaaatt attgataacc atttaacctt cctttctatg cagatataat ctattataac
     9421 ataaatcgct tacaaatcgt gtgggcataa aaacacagtc tgcaaaaaaa gccctgatag
     9481 gcatgcctat tagagcctcg aaaagaaaaa tattgaatca atctggtcgt acctcttgga
     9541 tcggtttgac acgaccgccg attcttttag caaaagcttc ggattctttt ttgtctttga
     9601 aaatgagcag attgtctaag tttttttccc gagtgcgaca gccagtttta tcgatgtaac
     9661 cgtgcattac tttaacgaca tacatgggga tcacctgctt ttttcttttc attatacgaa
     9721 ctactcgaca caatacaaga aaaatgctta gacggtcttt attaagaatt tacatcatcc
     9781 aaagaagcgt acgcttggaa taactgatct aactccggat tgactttgct cagcttcact
     9841 aatcgttgcg aatcagaaag taaaaaacag tgtttactac tgccagtagt ttttaatttt
     9901 ttgtcttcag ttcccacaat ttcgtagcta aaatccaacc gtgtccccgt atacttttgg
     9961 atagttggat ggatgctcac tttttctccg taatggatca tttctttata gctgcaattt
    10021 acttccagaa cggggatgat gatcccagat tcttcgattt ttttataggg aaaccctaga
    10081 tgtaccaata aggctacccg agcctcttca aaccagcgga tataattgct gtgatgaata
    10141 atccccattt gatctgtttc ataataaaaa ggttctcgca cataacctgt aaatttctcc
    10201 atgactcatg cctcgttttc cattgatttt ttacaaaaat aattcatcca tttcggaagt
    10261 gcttcgctga tttgtgtcgg caaaacaata tattgttctt ttgccagctg gtctccaatg
    10321 aaactatgaa gataaatagc tgctgcaatc gtttggtcat tttgaggaaa ctgggctaaa
    10381 aatcctgtga tcatcccagc taaagtgtca cccatgccgc ccgttgccat agcagcagaa
    10441 cctaaaggat tggtgtagaa gtgctggtga ccataaattt ttgtctcatg acttttcaaa
    10501 acgagcacag caccgatttc ctgttgcttc tttttgtttt ctttatcccg ttgttcttgt
    10561 atcggtagag aactcaaccg ttgccactcc atttgatgag gtgtaagtac agtttgttcg
    10621 ggatagggta gcaaaagatg attagcagca agtaaactta aagcagagcc atcaatcacc
    10681 agccattgtt cttttgattg gttttctagt acttgtttga aacgttccaa actcttagaa
    10741 gaagtaccca atccgggacc aatcaagagt acatctgctt gctttattac ggaaagaaac
    10801 tggtcagtat ccgtccaaac tacaatcatt gcttctggca gatgactgtg aagagcactc
    10861 cgattttttt catcagttac tacagtcgtg agacccgctc cgctgtgtac ggcagctaaa
    10921 gcactcataa tgattgctcc gccatattgt tcattgccgc caatcaaaac gatacgtcca
    10981 aatgttcctt tatgtgaaat ttttggacga ggtgtgatga ctcttttcac tagttgttcg
    11041 tttatcagca tctaaaaacc tccttttact cattatagcg tatatttttt ttagagaaaa
    11101 acagataaga aaaggaattg tcagattttt tgaactattt ttttctgtaa tttgtggtat
    11161 catgaacacg aagacttaaa taagtggagg attaacttta tgacaattga ttggcaaaaa
    11221 gaagtagaag cacgtaaaga agacttgctt gaagacttga aaaatctttt acgtgtcaac
    11281 agcgagcgcg atgactcaaa agtaacacca gatgcaccat ttggacctgg acctcgcgac
    11341 gcattgaaac atatgttagc ttatggcgaa cgtgatggat ttaaagtaaa aaatgtcgat
    11401 aactatgcag ggcatattga tcttggcgaa ggagacgaaa cgctagggat atttggacac
    11461 atggatgtcg ttcctgcagg agatggctgg gacacagatc cttatgaacc agtaatcaag
    11521 gatggaaaga tctttgcacg cggatcaagc gatgacaaag gtccaagtat ggcagcttac
    11581 tatgcgatga agatcattaa agaacttgac ttgaaactat caaaaaaagt tcgcttcgta
    11641 gtcggcagcg atgaagaaag cggctggggc gatatggctt attatttcga acatgaagaa
    11701 gaaccagatt ttggtttctc accagatgct gaattcccaa tcatcaatgg tgaaaaagga
    11761 aatgtttctt tagctttacg cttcaaaggc gacaatgcag gagattatgt cttgaaatct
    11821 tttgtttccg gacttcgcga aaatatggtg ccaggaacag caactgcagc acttcaagta
    11881 ccaagtgcag atgcagctat cgcgatggaa gaagcatttt accaattcat cgaagcaaat
    11941 ccagtaagcg gaacgattga agcggacaat acgtatgtaa aaatcgaatt agttggtaaa
    12001 ggcgcacatg gtgcaagccc acaatctggt atcaatgcag gttctttctt ggcattgttc
    12061 ttagataact atgaattcct aggatctgct aaacaattta tccatgtagc agctgcttat
    12121 gtccatgaag atttttatgg agaaaaacta ggagtcgctt atgaagatga aaaaatgggc
    12181 aaattgacaa tgaatgctgg actatttgct tttgaagaaa atggtacaga ggaagcgaac
    12241 ttcatcaata tgaatttccg ttttccaaaa ggtgtaacag tagacggatt gcaaagtgac
    12301 atcgaacaaa cagtggggca agaaggcgca acagttactc gaggtgctcg cgtaatggaa
    12361 ccacattacg taccaatgga tgatccattg gtggctacat tattgcaagt ctatgaagac
    12421 cacactggag aaaaaggcta cgaacaaatc atcggtggcg gaacttacgg acgtttgttg
    12481 aaacgtggcg ttgcttatgg agcgatgttc ccaggatata ctgatacaat gcatcaagca
    12541 aacgaattta tgagtcttga tgacttgttc agagcaacag caatctatgc agatgccatc
    12601 tatcgtttgg cgaaataatt aattaaaaag aggatcaaga attgcaaatt atttttgcag
    12661 tcttgatcct ttttttactt tctactgtgg tattcatgat aaacatgata tagattcaat
    12721 tcatattctt tcttagcgtt cgtgacgact gtatccaaaa tcaaccctgt tactaatgaa
    12781 agaagtgcta aagtcatcaa tccagtactt aaaatcgcag aagggatgcg tgtaataaag
    12841 cttgtcagca tatattcgcg aatcacagga atccccgtga ccaatccaaa taaaaagaag
    12901 aagatggtcc aaatcccaaa gaaaagcaaa ggcttataat ctttaaacat tttaataatc
    12961 atcatgatca ctttgaatcc gtcagagaat gtattcaatt ttgattcgct gccttcagga
    13021 cgatcacgat aatcgattgg gatttcaacg aacttgaatt ttttatctaa agcatggatc
    13081 gtcaattccg tttcgatttg aaaaccagaa ctcataatag gaaaactttt gacaaagata
    13141 cgattgaatc ctcgatagcc ggtcatgaca tcaagaattt ttgttttata cattctagta
    13201 attgtatttc taacaaggtt atttccaaaa tcgtggaaag gtctcttatt ctcatcaaag
    13261 taggttccat ttgataggcg atcaccaatg accatatctg cttcaccaga acgtaatttc
    13321 tcgagcaaac catgtacagc ttctgcagga taagtatcat cgccatctac cattaaataa
    13381 tagtcggcat cgatatcaaa gaacatttgg cggatcacat ttccttttcc ttgacgaggt
    13441 tcttttttta cgattgctcc accttgaacg gccaattcgt atgttcgatc ggtcgaatta
    13501 ttatcataga cataaatatc tgcttctggt aattctcgtt taaagtctgc aatgactgtt
    13561 gaaattgttg cttcctcatt gtaacaagga atcagaacag caattttttc ttgttgttcc
    13621 atttttcact ctccttaact atagaaaaca aaagctaaat taaaaatacc tcataatcaa
    13681 aggaaatgct agtttttccc gctaaatatc atttatttaa aaagaaaata ctggaatata
    13741 tgtttggaaa gctctatcta aaaaaccacc cactacaaaa gaaggacatg atacctctct
    13801 tttgaatagt gggtgcgtat tcgttctctc acttttttga cgtattaatc tgttgcacct
    13861 ttttttcttg aaatttctaa tacggttcct gtatgagcat ctgctaaaaa ttcataataa
    13921 acagtttcgc catcttctat tcgagaaaga cctccccgat aagcactcga ccgaacggcg
    13981 aatttcctaa taggttcctt ttcaaaagag atccaagaac cttcgattgc accttctttt
    14041 agaaaagatg acttgatatt ttcaagtact tggtctgctg acaatacttt tttgcgatga
    14101 taccaaatcg ttgaagctac cccgctcaat aatcctagac cgattccaac tgctaacccg
    14161 cctttaaaat agcttagttc gctttcttca tacatgtgga catacctcct tcttctcact
    14221 tgttactcct tgtgcttatt ttaccacact ctcatatttt ctttgaaata aataaccgct
    14281 accagaaaaa ataagctata ataaatacga tcggtctgca gaaaaaatca tattttatcc
    14341 gcagatcaat ataagtctga gaggagttta gtctgaaatt tagacgaata gcatatggaa
    14401 gaaaaaacat ttcaacgaat caaagagtta acagaattac aaggaacaag cggttttgaa
    14461 caagatgttc gcgcttatat ggaaagtcat atggaaccgc tggtagatga gttacagtta
    14521 gatggtttgg gcggcatttt tggtctgcgc catcatgaag aagcggatgc accccgtgta
    14581 atggtcgcag cacatatgga tgaagtcgga ttcatgctga cacaaatcca agaaaacggg
    14641 ctttttaaag tcgtacctct aggcggctgg aatccttacg ttgtttctgc tcagcgtttt
    14701 acacttaaaa cctctactgg taagaattat ccatgtattt cttcttccgt tcccccacat
    14761 ttattgcgag gaacaagcgg acaaaaatca gtagaagtaa ccgatatttt gttcgatgct
    14821 gggtttgaat caagagaaga ggcaatgagt tacggtgtcc ttccaggaga cacaatcgtt
    14881 ccttacgctg aaacgatcaa aacagccaac ggaaagaata ttatcagtaa atcatgggac
    14941 aaccgctatg gctgtacaat ggtattagaa gctttagaag cgttgcaaaa tgaaacatta
    15001 ggtcatacat tgattgcagg cgcgaatgtc caggaagaag taggcttgcg tggttctaaa
    15061 gcttctgtca ataaattcaa gccagatctg ttctttgcag tagactgctc tgcagcagat
    15121 gatacagtga cgaaaaatgg cacattcgga catttaggag aaggtacatt gatgcgtatc
    15181 caagatccag gtttgatcat gcttcctcgt ttgcgcgagt acttgctgga tatcgctgaa
    15241 acaaacaata tcccttatca atactttgtt tcaaaaggcg gtacagatgc aggtgctgct
    15301 catacacaaa acgaaggaat cccaagtaca gtgattggcg tagtgggacg ttatatccat
    15361 acgcatcaaa cgatgttcag tattcgcgat tttgaagctg cacgagaaat gttgattcaa
    15421 acactgaaag gtttggataa atcaacagta aatacgatcg tatacggaaa ataatgcgat
    15481 agtgaaacta taaaatctgg caaaagattg ctttttgcca gatttttttt ataaaaagag
    15541 gatcaatgtt tgttagttcg aaagaaaggt gtataattta attgaaaaat acataaacag
    15601 aaagtagagg acaaagaatg attatcccaa atagtttaga agaattagca ggctacgtag
    15661 aaactggaaa gagtgtattt ttctttactg ccggctggtg cggagactgc cgttttatca
    15721 aaccgcaaat gcctgaaatt gaaaatgact tttctgattg gcaatttatt gaagtagacc
    15781 gtgacaagta catagatgta gccgctgaat ggaacatttt tggtatccca agttttgtcg
    15841 tgatccaaga cggaaaagaa ttaggtcgtc tagtgaataa agatcgtaaa acaaaacagg
    15901 aaatcgaatc gttcctagaa agtgtaacaa aaggctgaca tctgttagga ggggtcaaga
    15961 tgaatcaaga atataagaat attttagtag gtatcgacgg tagtgaacaa gcacttgctg
    16021 ctttcaaaaa agcggtagaa gtggctcgcc gtaataatgg gaaagtatat gtagcaaacg
    16081 taatcgatca gcaattttat aactttatgg gctatgcacc tattgatcaa aatctagtcg
    16141 atcagcaaac tgaaacagcc aaggaaatga tcgaagaatg taaagaatat gggaaatctg
    16201 ttgattacac aaatatagaa ggaattattg catatggttc tgttcgtgaa tccatggcag
    16261 tcaaattgcc aaaaaaatat gagatcgatc taatcatggt tgggcagtca ggtctaaatg
    16321 cagtcgaacg ctttatgaca ggaagtgtag ccagctacgt gatacgtcat tcaccttgtg
    16381 atgtcttgat cgtttctgac gaagaagaac agccaagtaa ttaagcaagt ggaacttgta
    16441 cgaccttttt aatcaattaa ctaaaacagt aaagggactg tgacaaaagt cttgtcacag
    16501 tcccttgtta cgaataaatg gtgttccaga agaggcttct tcggaaataa agcatgaaaa
    16561 aaatttcatg cagtgaatgc aattttttgt taagataaat ctcagcaaag gataatggag
    16621 gaaagaacat gatttttgct tataacaaag cacacgtcgg agacaccttg atggttatta
    16681 tagccgacga taaaggaaca gaaaacactg ttgaacgcaa atggaatgtt gctcggatca
    16741 aagatgaaca aggtcaagtt gttgcttgga actttttcca tatctccgat catctaacaa
    16801 tcgaagggaa cggccaagta agcttaacag atgaggaatt agcgaaattg aatcgattga
    16861 tcaaagaagc aggattcgaa gaacagcttg aagctgatca tgaaccgaag atcgttgtcg
    16921 ggtacgtaaa aacatgcgtc ccacatcctg attctgacca tttatcgatt actgagacag
    16981 aagtagacaa tggtacagta ttgcaaatcg tttgcggtgc acccaatatc gaagctggac
    17041 aaaaagttgt cgttgcaaaa ccaggagcta tgatgccaga tggaatgatg atctggccag
    17101 gaactttgcg aggggtagat agctatggaa tgatctgttc cgctcgtgaa ttgcatttgc
    17161 caaatgctcc agcaaaaaaa ggaatcttag tattgcctga ggatgccgta gtcggagaag
    17221 catttccaaa ataaagaggt tgtgacaagt ctttgtcaca acctcttttt tattaatttt
    17281 cactattttg attttgtttt aatgccgatt gatcgatcgt caattcaaca gtagttgttt
    17341 tttctttcga gccttcatag taagtgattt tgacagaatc tcccactttt ttcttataca
    17401 aagcagattg aagttctaca ccagaagaaa cttctttgtc atcgatctta gtaatgacgt
    17461 cgtattgttt caatccagct ttttcagccg gtgtagctgt ttggacattt gtcacgatga
    17521 caccgttagt tacagatgaa ggaatcttca agatttgttc ttgttgctga gtagaaacgg
    17581 cagataagtc gaccattgtg atacctagag ctggacgtgt cacttttccg tctttttcca
    17641 gttgattgat gatattcact acatcgttac ttggaatagc aaatcccatt ccttctacgc
    17701 ttacattaga agtagattca gaagtacttg cgatcttact tgagttgata ccgataactt
    17761 gtccttcgat attaactaaa ggaccaccag aatttccagg gttgattgct gcatctgttt
    17821 ggatcgcatt gatattgact gtttcgttcg attcattcgt acttgtcact tgtcggttca
    17881 aggaagaaat gattcctgaa gtgacagaat ttgcatattc agatcctagt ggggaaccaa
    17941 tcgcaatcgc aggctcacca accttcagag cacttgagtc accaaaagag gcaactgttt
    18001 cgactttgtc tgaattgatc ttcaacaccg ctaaatctga ataagcatct gttccaacta
    18061 attcagcttt taccttcgta ccgtctttca tcaagacttc aagaccttgc tggccatcta
    18121 ctacgtgatt attcgtgact acataagcag tattcccgtc ttttttgtag atcacgccgc
    18181 ttccttcact ataagcttcc aaattactat catctgagct gctactttct tcttgctgcc
    18241 cgaatagtcc gccaaaaccg cttgattggt tttgactttg taagttgata acagaaacga
    18301 cagcgtcttg aactttatct accgctttgg tgatatcaga atctacattg actttgacat
    18361 tttccacaac tgtttcaccg gcagaattct gattgccgct tgtagcagta gaactattgt
    18421 tgccagtgcc catcgctgca taaaaaatgc cagctgtcag tagtcctcca acgatccctc
    18481 caacaagccc cagacctaac ttacgccaga ggctattatt tttacttttt ttcatctttg
    18541 gtgtcacatt ttttctatcc atgtgttcct cctccaatta cattcattta tctactgtta
    18601 gtatagtctt atttgttgac tttagtctac tttgagactg tgaacttttt atgaaaaact
    18661 ttaggaaaaa tcattgtttt cagatgaaaa tatttgattt tctttttcca cagatactgt
    18721 gtaaaactac gcggatagga ttggaaaaat aagggataaa acatatccac agcctgtgta
    18781 taaagtggat aaattcttat aaaacgtatg tttgcctaat gaatgtgatg aaataaggta
    18841 aaataacact gttttctgtg gataaacctg tggatgttgt gcataactgt tggggaaatc
    18901 tttttaacaa aaatagccaa aataaacata tagacgaaaa aattgattat aataaacaaa
    18961 gtgagcatga agaccaaagg agagaaaaca tgaatatcaa aattatctca gtaggaaaat
    19021 taaaagaaaa atacctgatc caaggtatca atgagtatgt caaacggctg aatgcctatg
    19081 caaaaatcga attgatcgaa gttcctgacg aaaaagcccc ggagaattta agcgaagcgc
    19141 aaatgagaca agtaaaagaa aaagaagggg aacggatctt agcgaaaatc aaagaacaag
    19201 aatacgtcta tgccttagca attgaaggga aaaatcctac aagtgaatct tttgctaaac
    19261 aaatcgacca attaggtatc caaggaaaaa gccaccttgt ttttgttatc ggcggctctt
    19321 taggactcag cgaagccgtc atgaaaagaa gcaatgctca gatctctttt ggaaaaatga
    19381 cgtatccaca tcaattgatg cgtctgatcc ttgtcgagca aatctaccgt gccttccgga
    19441 tcaatgcggg ggctccatac cacaaataat gatatttttt aagagaaaaa attatcccta
    19501 ttttaaataa tagcaatagg tagtggtagg gagaaattta taaataactt ttttatttgc
    19561 tttggtagcc aagtaagttg caaaagaaaa atagatactg ttaaaaagat aatgtagaca
    19621 tagaaacaaa tacgtaaaat tttagttaga ataatggcaa ggagaaaaaa catgatagat
    19681 gaaaaaaagt gtttaaaaaa tctattagag gatatggatg atattgaaga aaactatcta
    19741 aatggactgg gaaaacaaat atatttcaga tacttaagct atctaaaaat gaaataagac
    19801 atagtaattt tttagctttt ttgtttgctc caaatgaaac ccacaattta tctgatgagt
    19861 tttttaaaat gtttttgaaa aggtatatag attctaatga tgataccaaa gctgctatca
    19921 attattttga tgctttactt aatagctatg aagatttaat tgtttacaga gaaaataaga
    19981 atattgatat tttattggta tcggaaaaaa ataaaatagt ggtgtgcatc gaaaataaaa
    20041 ttctgtcttc ggagtctgga ggg
//
LOCUS       DAZAFF010000049        19635 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000049 DAZAFF010000000
VERSION     DAZAFF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.049"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACG9BB_001675"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACG9BB_001675"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFN2837616.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="ACG9BB_001676"
     CDS             complement(1174..1869)
                     /locus_tag="ACG9BB_001676"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFN2837617.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="ACG9BB_001677"
     CDS             complement(1882..2598)
                     /locus_tag="ACG9BB_001677"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFN2837618.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="ACG9BB_001678"
     CDS             2928..3536
                     /locus_tag="ACG9BB_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFN2837619.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="ACG9BB_001679"
     CDS             3861..4184
                     /locus_tag="ACG9BB_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFN2837620.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="ACG9BB_001680"
     CDS             4366..5736
                     /locus_tag="ACG9BB_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFN2837621.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="ACG9BB_001681"
     CDS             complement(5866..7167)
                     /locus_tag="ACG9BB_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFN2837622.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="ACG9BB_001682"
     CDS             complement(7521..9512)
                     /locus_tag="ACG9BB_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFN2837623.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="ACG9BB_001683"
     CDS             complement(9656..9922)
                     /locus_tag="ACG9BB_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN2837624.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACG9BB_001684"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACG9BB_001684"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFN2837625.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACG9BB_001685"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACG9BB_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFN2837626.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /gene="def"
                     /locus_tag="ACG9BB_001686"
     CDS             13266..13829
                     /gene="def"
                     /locus_tag="ACG9BB_001686"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFN2837627.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="ACG9BB_001687"
     CDS             complement(14082..14603)
                     /locus_tag="ACG9BB_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFN2837628.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="ACG9BB_001688"
     CDS             complement(14606..15448)
                     /locus_tag="ACG9BB_001688"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFN2837629.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="ACG9BB_001689"
     CDS             complement(15441..16166)
                     /locus_tag="ACG9BB_001689"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFN2837630.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="ACG9BB_001690"
     CDS             16342..16638
                     /locus_tag="ACG9BB_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837631.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16894..17068
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17206..18798
                     /locus_tag="ACG9BB_001691"
     CDS             17206..18798
                     /locus_tag="ACG9BB_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFN2837632.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="ACG9BB_001692"
     CDS             18811..19551
                     /locus_tag="ACG9BB_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN2837633.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5620 a         4028 c         3725 g         6262 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagccatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaagta aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaga
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtgtctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caaccaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacagactc ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcta tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgagcaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaccagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatca ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt ctgaa
//
LOCUS       DAZAFF010000050        19474 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000050 DAZAFF010000000
VERSION     DAZAFF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.050"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..3654
                     /locus_tag="ACG9BB_001693"
     CDS             127..3654
                     /locus_tag="ACG9BB_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFN2837634.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="ACG9BB_001694"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="ACG9BB_001694"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFN2837635.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="ACG9BB_001695"
     CDS             7442..7783
                     /locus_tag="ACG9BB_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFN2837636.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="ACG9BB_001696"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="ACG9BB_001696"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFN2837637.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /gene="pheT"
                     /locus_tag="ACG9BB_001697"
     CDS             9140..11560
                     /gene="pheT"
                     /locus_tag="ACG9BB_001697"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFN2837638.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACG9BB_001698"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACG9BB_001698"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFN2837639.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="ACG9BB_001699"
     CDS             12994..13731
                     /locus_tag="ACG9BB_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN2837640.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="ACG9BB_001700"
     CDS             13745..14560
                     /locus_tag="ACG9BB_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFN2837641.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="ACG9BB_001701"
     CDS             14573..15220
                     /locus_tag="ACG9BB_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN2837642.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="ACG9BB_001702"
     CDS             15236..15892
                     /locus_tag="ACG9BB_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN2837643.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="ACG9BB_001703"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="ACG9BB_001703"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFN2837644.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="ACG9BB_001704"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="ACG9BB_001704"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFN2837645.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="ACG9BB_001705"
     CDS             18234..18752
                     /locus_tag="ACG9BB_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN2837646.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /gene="cbpB"
                     /locus_tag="ACG9BB_001706"
     CDS             18859..19359
                     /gene="cbpB"
                     /locus_tag="ACG9BB_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFN2837647.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6552 a         3344 c         4181 g         5397 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaag
//
LOCUS       DAZAFF010000051        19330 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000051 DAZAFF010000000
VERSION     DAZAFF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.051"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..585
                     /locus_tag="ACG9BB_001707"
     CDS             19..585
                     /locus_tag="ACG9BB_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2837648.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            730..817
                     /locus_tag="ACG9BB_001708"
     tRNA            730..817
                     /locus_tag="ACG9BB_001708"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:762..764,aa:Ser,seq:cga)
     gene            1153..2127
                     /gene="bsh"
                     /locus_tag="ACG9BB_001709"
     CDS             1153..2127
                     /gene="bsh"
                     /locus_tag="ACG9BB_001709"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFN2837649.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            2452..4347
                     /gene="asnB"
                     /locus_tag="ACG9BB_001710"
     CDS             2452..4347
                     /gene="asnB"
                     /locus_tag="ACG9BB_001710"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFN2837650.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(4542..4988)
                     /locus_tag="ACG9BB_001711"
     CDS             complement(4542..4988)
                     /locus_tag="ACG9BB_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFN2837651.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            5216..5365
                     /gene="rpmG"
                     /locus_tag="ACG9BB_001712"
     CDS             5216..5365
                     /gene="rpmG"
                     /locus_tag="ACG9BB_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN2837652.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            5453..5971
                     /locus_tag="ACG9BB_001713"
     CDS             5453..5971
                     /locus_tag="ACG9BB_001713"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFN2837653.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(6048..7004)
                     /locus_tag="ACG9BB_001714"
     CDS             complement(6048..7004)
                     /locus_tag="ACG9BB_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2837654.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            7170..7976
                     /locus_tag="ACG9BB_001715"
     CDS             7170..7976
                     /locus_tag="ACG9BB_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2837655.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            7973..8962
                     /locus_tag="ACG9BB_001716"
     CDS             7973..8962
                     /locus_tag="ACG9BB_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFN2837656.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            8959..9963
                     /locus_tag="ACG9BB_001717"
     CDS             8959..9963
                     /locus_tag="ACG9BB_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFN2837657.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            10095..10637
                     /locus_tag="ACG9BB_001718"
     CDS             10095..10637
                     /locus_tag="ACG9BB_001718"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFN2837658.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            10657..11355
                     /locus_tag="ACG9BB_001719"
     CDS             10657..11355
                     /locus_tag="ACG9BB_001719"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFN2837659.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            11376..11588
                     /locus_tag="ACG9BB_001720"
     CDS             11376..11588
                     /locus_tag="ACG9BB_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFN2837660.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            11588..12550
                     /locus_tag="ACG9BB_001721"
     CDS             11588..12550
                     /locus_tag="ACG9BB_001721"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFN2837661.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            12568..12963
                     /locus_tag="ACG9BB_001722"
     CDS             12568..12963
                     /locus_tag="ACG9BB_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFN2837662.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            13126..13491
                     /locus_tag="ACG9BB_001723"
     CDS             13126..13491
                     /locus_tag="ACG9BB_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFN2837663.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            13488..15299
                     /locus_tag="ACG9BB_001724"
     CDS             13488..15299
                     /locus_tag="ACG9BB_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFN2837664.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(15361..15528)
                     /locus_tag="ACG9BB_001725"
     CDS             complement(15361..15528)
                     /locus_tag="ACG9BB_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFN2837665.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            15777..16766
                     /gene="galE"
                     /locus_tag="ACG9BB_001726"
     CDS             15777..16766
                     /gene="galE"
                     /locus_tag="ACG9BB_001726"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFN2837666.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            16778..18262
                     /gene="galT"
                     /locus_tag="ACG9BB_001727"
     CDS             16778..18262
                     /gene="galT"
                     /locus_tag="ACG9BB_001727"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFN2837667.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            18286..19266
                     /locus_tag="ACG9BB_001728"
     CDS             18286..19266
                     /locus_tag="ACG9BB_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2837668.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         6108 a         3298 c         4071 g         5853 t
ORIGIN      
        1 agaagtgggg gagttttaat gagaagaaaa gtctatacca aggatcaaat tctaaaagct
       61 gcttatgatg ttgttgcaaa ggaagggttt aaaggattta ctgcgagaaa tattgctaaa
      121 aagatgggga tttcgacaca gccgatttat ctggaattca aaaacatgga agatttaaaa
      181 aatacgttgc tggattctat ctttgaacaa ctaaaagaag atgttttccc tattgaacgt
      241 actggtgata aaatcattga tctcggtttg aactatatct actttgcaaa aaagcatcat
      301 agtctttaca tggcattgta cattgatgaa catggggacg gaaagaaaat gcatgatttt
      361 tcatacaatt atttccgtga aatagttaaa gaagatgaaa gatacaatca attatcggat
      421 gaatatgtcg atgctctgca tactggtaca tgggtagcag tgactggctt agcgtcattg
      481 atgtcatcaa acatcatgca tccaacagaa gaacaaatca ttgcactgat caaacggaca
      541 attgactcga ttttagcatt agataatcct gaaataatca gatagtgttc tatcagtatg
      601 aatttgatcg gtaagtccgt tggctgcaaa ctgtcaatgg acttttttgc taaaaaaagc
      661 gatttctgtt atttatctat tagaaatagg cttgacattt cgtgaaaaaa gtttaaaaat
      721 agtacttacg gaaggttggc agagtggtaa tgcaccggac tcgaaatccg gcgaaccggc
      781 taataccggc gcgcaggttc aaatcctgta ccttccttat atcaacgatt agaggttgtg
      841 aagaagctgt cctgcatcaa gtaataagaa cggccaaaca aaaatagctt ttcctatttt
      901 ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt gttcggaata
      961 aggggctgtg acaagacttt tgtcacagcc ccttattgtt gtacttttaa cctacaatat
     1021 tttttgattc cccctgagta ctatttttta taaatgctag taaatcaatc ttctttggcc
     1081 ttttttttga taaaaaaaaa tactagatgt aagataaaag tgtaaaaacg atttgtttta
     1141 ggagggaaaa cgatgtgtac cgcaatcact tatgcaacga aagaccatta ttttggaagg
     1201 aattttgatt atgagatgtc ttataatgaa gtagtcacta ttactccaag aaattatcgt
     1261 ttcgatttta gaaaggtaaa aaatttagat aaacattatg caatgatagg aatagcagct
     1321 ggggtatcca actatccact ctattatgag gctacaaatg aaaaagggtt gagtatggca
     1381 ggactgaatt ttcctggcaa tgctgattat aaagaattac aagaaggaaa agataacgtt
     1441 gcaccatttg agtttattcc atggatacta gggcaatgct caaccataga cgaagctaaa
     1501 gaattattgg ctactatcaa tctagtcaat atcgatttta gtgaaaaact acccttgtca
     1561 cctttacact ggttattagc tgataaagaa aaatcgatag taattgaaag tatgaaagat
     1621 ggacttcatt tatatgataa tcctgtcggt gtgttgacaa ataatccgcc atttgattat
     1681 caattattta atctgaacaa ttatcgctcg ctatcaaatg gaacaccaga aaatcatttt
     1741 tcaaaccaga tcagcttgaa tgtttatagt cgtgggatgg gaggattggg cttacctgga
     1801 gatctctctt ctgtttctcg atttgtaaaa gccacattta ctaaaatgaa tgctgcatca
     1861 ggtgattcag aatctgaaag tatcagtcaa ttttttcata tactaggctc agtcgagcaa
     1921 caaaaaggag tatgcgatac tggagaagga aaatacgaat atacgattta ttcttcttgc
     1981 tgtaacgttg ataaagggat ttattattat cgtacgtatg aagacagtca aattactgct
     2041 gtaaatataa ataaagaaga cttggatcgt catgaattgg tcagttatcc agtcataaaa
     2101 gagcaacaga taaactatgt caattaatta atgaatagat ccgtttggta tcatattttc
     2161 tgatacagtc ataattagac acgatcttta ggtcgtgtct ttttttgctg ttttttttgg
     2221 aaatagcatc cactaatatt tttttaacac gaagagaata aggtagcctc aaaaaagaaa
     2281 acatggtaaa ttagatattg ggactttatt ttctgtaaaa acagaaactt tcatcttttg
     2341 ttttcacgtg acgaaattgt aagagagaaa atattcgtcc gctatttatt ctatgataag
     2401 atgcatatga atgaaataaa cagtaatttt atattaggat gtgaaagtta aatgtgcggt
     2461 attgtaggtt ttgttaacga caaggacaac aaaaaaacaa ttattaatac aatgatggat
     2521 cgaatcgttc accgtggacc taatagttca ggagaatata tcgataaaca tgttgcttta
     2581 ggattcagaa gattaagtat tattgactta gagggcggta ctcagcctat ttacaatgaa
     2641 gatcggacaa agatcattat ttttaatggc gaaatctata attaccagcc tttgagagaa
     2701 gaattgattg cagctggcca cgtttttcaa acacatgcag atacagaagt ccttttgcat
     2761 ggttacgaag aatggggaac agaattattg caaaagatcc gcggaatgtt tgcttttgct
     2821 atttgggata atgaaaagaa tgagttattc ggtgcaagag accactttgg gatcaaacca
     2881 tattattatg cagaaatgaa cggaacattt atgtttggtt cagaaatcaa aagttttctg
     2941 cctcatcctg attttaacaa agaattgaac aaagaagcgt tgaaacctta tatgactttc
     3001 caatattctc cattaaacgg cgaaacattc ttcaaagatg tctatcgttt gcctgaaggc
     3061 cattattata cgtataaaga tggaaaacta gacattcagc aatattggga tgcagatttt
     3121 gaaacaaaag aaacgcattc ccgacaagaa tggatcgaaa aaatcgacga aaccgttcaa
     3181 gcttctattg aagcacatac ggtcagtgat gtagaagttg gttctttctt atccagcggg
     3241 gttgattcca gttatgtaac ctctgtattg aaaccagatc attctttctc aatcgggttt
     3301 gatgataaaa cctataatga agcgattgaa gccagaaaac taaccgaact attggactta
     3361 gataatacag cagcagtgat cgacggagac atgtcgttca aagcattccc tttgatccag
     3421 tatcacttag atgaacctga ttcaaatcct tcttgcgtac ctttgtactt tttagcaaat
     3481 cttgcttcac aaagtgtgcg ggtagtccaa tctggagaag gggcggatga actgtttgcg
     3541 ggataccaaa cgtatggatt ccatacgaat tcaaaattca tccgtgtgat tgctcaagga
     3601 ttgaaaaaat taccaaaagg aacacgctat aatttaggac gtaaaatcgg aaaaatgaaa
     3661 aatttccatg gacggatcca tttatatgaa tcacttgctc ctgcaaaaga atattttatc
     3721 ggtcatgctc gagtgttcga agaatcagaa gcatcagaag tgttaacacc aaaatatcaa
     3781 acagcaccat ctgtggatga aatcatgact gttcattacg agaaaacaga aggaatcaaa
     3841 gatgaagtca ataagatgca atatgtcgat cttcatcaat ggatgccaaa agatatcttg
     3901 ttaaaggcag ataaactttc tatggcaagt tctttggaag tccgagtacc tttattggat
     3961 atcgaagtca tgaaattggc tcaacaaata ccaagtaagt ttttattgaa tcaaaacaac
     4021 acaaaagata ttttcagaca agctgcgaac aaacatcttc cggaagaatg gtcgaatcgt
     4081 gtgaaattag gattccctgt gccaatcaaa gcttggttga aagaagaaca cggctacgaa
     4141 caagtcaaag cattattcga agcagatttt gcaaaagaat tttttgatca agaaaaaatc
     4201 atgaagttgc tagatgacca tcacgaagga cgtaaagaag agcaaagaaa aatctggaca
     4261 atcttcagtt tcttgacatg gtataaagtc tactttgtag atgaaacgat tccgcaagca
     4321 gaagcaatcg actatgtgac tgtttgatga aaatcaaata tcaataaaaa agaggttttc
     4381 tcctaccgtt taacactgag tgataagaaa caatttcaaa aaacagctct tcccgtttta
     4441 tagatattat gacttatggt tcggagttgg cagaggaaca ccattgtttc gaacataagg
     4501 gggttgtgac acgattttcg tcacaacccc tttacatatg ttcaataatc gatttctttt
     4561 tcttttgtcc catctttttt ataaatgaac aaagttgatt ctttgttttg tgctacttgc
     4621 tttccttttt cgattgcttc tttttttgta tcaaaatggt tgcttgcctt ttttgcccct
     4681 ttagatttca cgatccattg ttcttcctca tattctacga tcacatctga atcaagcagt
     4741 tttccagcac gaggattcgt atcgtgttca tccgtcttgc ttggattttt ttctttttcg
     4801 aaagttcgtt tttctgattc actagcatct tcataccatt tttttgcttg atcaatggca
     4861 attgggatcg cacggttatc agggtaccct tcatctaata atgcatttgc gatgtcgatt
     4921 gctttttttc ttgtcaattt atctaagttt ttcattgatg caggaaagtc cttcatgttc
     4981 catggcatat tagtatcccc ctttattaaa aaacataatc tatataaaca agtgtaaggg
     5041 tatttataca aaaagaaaaa gattttgttt gagaagtaaa accgttgtat ttatcgtcaa
     5101 gtagttttac ttaacaaaat cgtgaaaaac actagcaatc tagaaaaaat catggtaaga
     5161 taatcaagtc tgagagtcat cttagaggat tcttgtaaaa atgggaggga aaaacatgcg
     5221 cgtaaacatc actttagagt gtacttcttg taaagaacgt aactatctaa caagcaaaaa
     5281 caaacgtaac aatcctgatc gtttggaaaa acaaaaatat tgcccacgcg aacgcaaagt
     5341 tactttgcac cgtgaaacaa aataatgttt ttaaagttct caattccttg ctggtcaaag
     5401 aattgagact ttttttatct caaaacatct aaaatcataa ggagcggata atatgtttat
     5461 ttccatgtgg gcacaagata aaaatggtct gatcggcaaa aatggtttgt taccatggcg
     5521 attgccaaat gatatgcgtt tctttcgcga acatacaatg gatcgaattt tagtgatggg
     5581 aagaaagacc tatgaaggaa tgggggatct ttcacttcct tatcgccata tcatcgtgtt
     5641 gacaacgcaa ccaaattttc gcacgaaaga aaatgcggaa gtaatgcatt cgatcgatga
     5701 acttttgtcg tatagtcaaa cgatctcaga agatatctat gtttcgggtg gtagtcggat
     5761 ttttcaagaa cttcttcctt acactggtcg gatctggcga acattgatcg atagcacgtt
     5821 tgaaggcgat acgtatattg ggaatattga tttttcagac ttcgccctta tagaggagta
     5881 tgaggggata acggatcaag aaaatctata tgcccatcgt tttcaaaaat gggaaagagt
     5941 aaaaaatagg gttgcttcaa aagaagaata aaatctatgc gtctagtttg ttaataagaa
     6001 aaaaagcatc tcacgactaa tcgtgaagat tcttttttta gttaaaatca atttagtttt
     6061 tctttgatat cattgaccat ttcctgactg gcaataggtc cgttatatag ccaactagat
     6121 tcaggtccga attgcagtac atgattgtct gtcactgctt taagattctt ccagcttggt
     6181 tcattgaaca tggcagcatc tttgtcactg ttcacaagga tgatgtagtc agcatctaat
     6241 tcagctagct tttcagagga aacagcagac cagtcagaag tggctttttt ggagacctct
     6301 tccactagat caggaacacc aaattccaga tcgtcgtata cgattcttcc acttgaccga
     6361 tttttgctga ccataaaggc tgagttgttg gtcacccaca acactgcagc tgtcttgttc
     6421 tcgattttat ctgctagttc ttctcgagtt ttcttgatgg atttttgata atcggaaatg
     6481 attttttctg cctctttttc ttttgccaaa gctttgccga catcttttag ttgttcttcc
     6541 catgtgacat ctgttccgtt ttttacaaca taagttggcg cgattttgct atattgttcg
     6601 tatttcccgc cttcgacagt agcggaagaa gaaatcaaca gcaaatcagg ctcaaagctt
     6661 aacactttct cataaggaag gtcataagaa atcgttggga catcctttaa atcatcttgc
     6721 agataatctt gaatggaacc actgccaaca gtccactggg cgataggttt ttcccctagt
     6781 gcaatcagat agtcttctaa gtaactgccg atgatccgtt tgggcgcgtt tggaatctct
     6841 actttatgtc caagtgtatc tgtgactgta tgagtactat tggatgaagt cgtcgaattg
     6901 gcagttgtag ctgtttcttt atctgaagag ggggagcaag cgctcagtac tccagccagc
     6961 agcaatatgc tagataatgt gaatactttt gtttttaatt tcatttcttt cctcctaaca
     7021 taattgagaa tctttctcat tggttattat tcctgatatt tcgaacgctg tcaaccgctt
     7081 catttggcta tagatatgaa agtagcgctt gacgaaatgt tgaaacagcg tcataattga
     7141 gaatgatttt cagcgaaagg atgggtgaaa tgacgagatt aagagcagct gacattgcag
     7201 ccggctacga aaacaaaaaa atcattgaaa acatgtctgt tacgattcct gaagggaaaa
     7261 tcactagttt gattgggcca aatggaagtg gaaaatctac tttattgaaa agttttaccc
     7321 gtatactacc tgtgaaatca ggaaagatca ttgtcaataa ctctgatatc tctacataca
     7381 gttcaaaaga acttgcaaaa aaaattacat tattagcaca aactagtgaa caccctacag
     7441 gtatgacagt agaagaagta gtatcttacg gacgataccc ttatcaaaaa ctgttcagtg
     7501 gcatggacac gcaagatcat gaagcaatcg agtgggcgtt ggatgctaca ggtctagctg
     7561 gatttcgtat gcgggatatc gcaacgctgt ctggtggaca aacccagcgt gtctggatcg
     7621 ctatggcgct tgctcaggaa acagatatct tgatcttgga tgaaccgaca acatttttag
     7681 atccggcaca ccaattagag atcctacagt tattgactga aatcaatcaa atgaaacaaa
     7741 caacgatctt gatgtcgatt catgacatta atcatgcttc acgtttttca gattatttat
     7801 tagcaatgaa aaacggtaag ttgatcacag aaggtacacc tgaagaagtg atcacaaaag
     7861 agtgtatgaa aaaaatattt gagattgatg caacgattgt cacgcttcca agaacaaata
     7921 aaccagtttt tctaacctat gatttgttgc caaaagagca ggaggatcaa ttatgaggac
     7981 tatgaagaaa aatagactcc tgcttttggt ctgttttgtt cttttgatta ttttttttgt
     8041 tgctctttca aatggcgcag cgactgtggg aaaagcagat ttttggcagg cgttgtttga
     8101 ttttgataga tcaaaacaaa gtcaccaaat catacgaaat attcgtttgc ctcgtgtggc
     8161 gggagcattt cttgtaggga gttgttttgc attgagcggt gcattgatgc aaggagtgac
     8221 aagaaatcct ttagcagatt caggcttact aggaatcaac gcgggtgctt cgttggcaat
     8281 ggcccttagt tttgcttttt ggcctaaagc ttctgatttt tctgtgtttc taatgtcatt
     8341 ggcaggttct tttgctgtaa ctttgctcgt atttggtttt ttgcagtttt cgaaaatggg
     8401 catgaatcct ttacagctga ttttagctgg cgtagctatt agttcttttt ttactgctat
     8461 cagccaagca cttacccaat tctttaatct gcagcaggat cttgcttttt ggtttgtagg
     8521 cggagctgcc aatatcacat ggaaacaatt agcagtattg tttcctttat atatacttgc
     8581 tgttttggtt tctttttcat taggaaaagc aatatctttg attcatttga gtgaaacaca
     8641 tgcattagct cttggaagtc atcctaaacg aacgcggttg attgttttct gtctagttgc
     8701 agtgttagca gccattgccg tttcgttagt cggacctgtt tcatttattg ggctgttggt
     8761 tccgccagtt gttcgcggaa tggttggaac ggactatcgt tatgttctac ctgtctcttt
     8821 tttagttggc ggtggattgg ttatgttggc ggattttaca gcgaggatga tcaatccccc
     8881 ttttgaaaca ccttttggat tagtcattgc attgattggc gttccgtttt tactgataca
     8941 agtaaggagg gaacaacggt gaaaaaaata tattggacgt tctatttctt gctttttgta
     9001 cttgtcatcc tgtctatagg tagtttaatg attggtacgc cttttattta cctggatcag
     9061 ctttttgcca cgttcatagg gaaagaatca tcttctttgg cgctgattgt tagagaattt
     9121 cgagttcctc gattactgat cagtttatta gcaggagctt gtcttggcgt ttctggttat
     9181 atcttgcaag gagtaacaag aaatgaattg gcagattcta gtatattggg cattaatgcc
     9241 ggagcaggat ttttagtgat gctttattta ggtttctttt cgcactattc gctgccgttt
     9301 ctattgctag ttgtagcctt tctaggtggt gttttagctg cttgctgtgt ctatcttgcg
     9361 gcctatgatc gaaatggctt tttgggtatg aatcgaatct tattgtccgg tattgccgtt
     9421 aatgccggcc tttctgcatt aacattgcta tgcacgatcc aattatctaa ggaaaattat
     9481 ggatttgtga atgcatggtt agcgggatca atctggggtg cgagctggcc ttatgtttgg
     9541 gcattgcttc catgggcggt tatcttaatt ccgctaggag gactttatgc acagaggatt
     9601 ggattgctat catttggtca agaacgcgcg caaggattag gccttaacgt ggctaaaagt
     9661 caaaaaatcc ttttgttgtt agctgttgct ttggcaagcg gtagtgtagc ggtgacaggt
     9721 agtttatctt ttgtaggact actagctcct catgctgcga agctagtcgt acgtaaagaa
     9781 aataactgga cctttgttct gagcggtttg ttcggttcca tttttctgct tagtgcagat
     9841 atggttgcta gagtgattct accaagtggg gaagtaccta caggaatttt gattgccctt
     9901 tttggtgcgc catattttct gtatgcactt tttatcagac aaaaacacgc gctgaacagt
     9961 tgaaatttat taaaaaagga aggccaatca tttctaggtc ttctttttat tctatttatg
    10021 catttatttg aaagttcgat tgctattttt ctttttttcc tataccattt tatgagagaa
    10081 ggaagggtga gtttttggac caattgaaag taagattacg ccggaaagga atcgaaaagc
    10141 tgaaaaatct ggccaaaagt ggcaagaaaa aggaaaaaga acagcgaatc tataaacagc
    10201 tattcgccag cgagtactgg aagtcagcaa aagtgatagg gacgacttta tcgaatgaat
    10261 ttgaactgga tacacagccg gttatccaac gagcagaaaa agaacaaaag aaaatagttg
    10321 tacctaaaac tttgccgaaa cgtcagatgg cgttttatga aatggatgaa cagtccgttc
    10381 ttgtacgttc gagttttggt gttcttgagc cacaatcaga gaagctctat ttgccggaga
    10441 tgatcgatct cctcattgta ccaggtatta tctacaatag agccggttat cgaatcggtt
    10501 ttggcggtgg ttattacgac cggtatttaa ataattttca taaaaaaaca tgtagccttg
    10561 tttttcgtga acaattgaat gaacaatggc aaccggagag ctttgatcag aagatccaac
    10621 aattgtttat tgactaaaat gaaatgggag gttttcatga attatcaaca gcaaataaaa
    10681 ttgcgtgcat ggttaagacg accgttatgg acctatgctt ttttagggat acagacagcg
    10741 gtcttcctca tgatggaatt atttccgcga cttgagatcc cttattatgc cggaatgtat
    10801 ggtccttatc ttgttcactt caatgaatgg tggcgtttag tgacaccaat cttcatccat
    10861 tttggactga tgcatttcgt aatgaactca ctgatactgt attttatggg gcagcaaatc
    10921 gaggcaatct atggccattg gcgttttttc ttgatttata tgttcagcgg gataatggga
    10981 aatacagcaa gctttgcttt taacgaagcg aatgttctgt ctggtggtgc gagtacttcg
    11041 atattcggtt tgttcggtgc gttgtttatc ttagggtttc attttaaaca taacccagcg
    11101 atccagcagc tagtcagaca ttttctttta tttattgtgt tgacatttgt ttttggtctt
    11161 ctggatacct ctgtagatgt ttggggacat gtcggtgggc taattggcgg attagtgtta
    11221 gggaatgttt tgggtctacc gaaacaacag acaagctatt cgatacacca aaggattctt
    11281 tctacactag tttttgtatt tctttttgta atatgtattt tactgggttt aaaaaaatat
    11341 ggtttacttg tataatatga aggaagtgct taaagatgga gagcttatat gatgtacagc
    11401 agctgttcaa aaaatttggc atctttatct atgtgggtgc acgcatctat gacattgaat
    11461 taatgactat cgaattgcgt aatctgtatg atagccgttt gattgatcat gatacataca
    11521 tgacagcgtg gcgcatatta aaaagagagc accgaattga agaaagccga aagaaaggaa
    11581 atttgtaatg gacaagaaga ttattgggat tgatttaggt ggaacaacag caaaatttgc
    11641 aattttaaca ccagaaggag agattcaaca aaaatggagc atcgatacca atattttgga
    11701 tgatggtaag catatcgttc ctgaaatcat tgaatcaatc aaccatcgtt tgaatttata
    11761 taatatgaaa gccgaagact ttatcggaat cggcatgggt accccaggaa gcgtggacag
    11821 cgaagctgga acggttattg gtgcgtataa cttaaactgg acagaagttc aatttgtgaa
    11881 aaaactgatc gaggcaggaa caggaatcaa attcgctatc gataatgatg cgaatgttgc
    11941 tgctttaggt gaacgatgga aaggtgctgg agaaaacgat ccagacgttg tattcgttac
    12001 tctaggaaca ggtgttggcg gtggtatcgt tgctggcgga aatttgattc acggcgtgag
    12061 cggagcaggc ggagagatcg gccatatcac tgtggatcct gaagggttcg aatgtacttg
    12121 cggtaaacgc ggatgtttag aaactgtgtc tagcgctact ggtgtggtac gtctaggtcg
    12181 ttacttggca gaagaatacg caggagattc aaaactgaaa gcaatgttag acaacggtga
    12241 agaagtaaca agtaaagata tctttgaaat ggctcaagaa gacgatccgt ttgcattgat
    12301 ggttgttgat cgcgtatgct tctacttagg tttagcttgc gccaacttgg ggaatacatt
    12361 gaatccgtca agtattgtgt taggtggagg agtatcggca gcaggagaat ttttacgtag
    12421 ccgtgtagag aaatatttta aagaatttac tttcccgcaa gtaagagaat caacaaaaat
    12481 caaattagca gagcttggta atgaagctgg tgtcattggt gctgcctcat tagccttgaa
    12541 atttgcttaa aaaaacggag gcattacatg gttttatggg taattaatat cattttgcta
    12601 ttgattgtgc ttgcaatcat cttttgggaa gtctatctac gagtaatggc caagcgttca
    12661 gcaacgactt tgacagaaga agaatttcga gaaggaatga gaaaagcaca agtaatcgat
    12721 gttcgcgaaa aagatgtgtt tgatgctggg catattttag gtgcaagaaa catcccttac
    12781 actgttttga aagattcatt aggatctatt cgaaaagacc agcctgtcta tatttacgat
    12841 caaaagaaaa gcttgagcat ccgtacagca aataaattac gtaaagcagg ttatcaagag
    12901 atttatttac ttaagggtgg atatgacgga tggtctggaa aaatcaaaag taaaaaagta
    12961 taaaacagat gattgtatga cagctttaaa aaccgaatca atcattagat tttgaccaag
    13021 atcacatgaa cggcgcaatg gatagttgtg ccgttcattt tttttatttt gtgctattat
    13081 ctacatatat aagctaagcg aaaaggcaga aagaaggaaa caactatgat caaattaaaa
    13141 cgtgtttacc ttgatgctga aaaagaggat ggctatagaa tactcgtgga taggttctgg
    13201 ccacgaggaa tttcaaaaga aaaagcgcag ttagatctgt ggttgaaaga aattgcacct
    13261 agtaatgaag cacgcaaaac ttttgaccat gtaccagaac gatttgcttc atttgcaaag
    13321 aaataccagc aagaattgat tttcgaagaa gaaaagaagc aagctatttt taaactcagt
    13381 caacttatga aagaacatcc agtcattact tttgtttatg gtgcaaaaaa tgaacatcag
    13441 aaccatgctg ttttattgaa aaaatttatc gaaatggagg catgcaaatg aaaataggga
    13501 tcattggtgc cggacctaga ggattaagta tagcagaaag gattctccat aataatcagg
    13561 acagacattc tcttgaactt tttcttttcg atcccaatgg tcctggaggt cgagtttggc
    13621 ggttgaatca gccaccggag ttattaatga attctgtttc tcaacaagtg acgcttttta
    13681 cagatgaaac attgtccagt ggaggattgg taacacaggg acccaacttg tttcaatgga
    13741 gtaaaggacc tgctacagat tatatcgaac agacggtaac caaaaataag caactaatta
    13801 tagaagaaat caatcgtctg gaaaaagatc agcaatctac tcgatgttta tacggactct
    13861 atcaacgctg gtttttctca aagttgcaag aagaatttcc agcttctatt cagtttgttt
    13921 attctttagt aaaaaaagtc agcaaaagaa aagatggtta tcttttacag acagatcagc
    13981 aaagctattc tatcgatcag cttgttttag caagcggtca ctgggaaaat gatgagacag
    14041 aagaagaaag agtgtgggta gactacgcaa agaaaaatga tttactgtat cagcttcctt
    14101 ctaatcctgc agatgtcgat ctttcgaaga taccgcctaa agaaaatgtc tttttaagag
    14161 gtctaggctt aagttttttt gattatgtcg gattgtttac agtatcaaga gaagggcgct
    14221 tcgagaacga aaaaggaaaa cttgtctatc atccatcagg caaggaaccg attgtataca
    14281 gtggatcaag aagggggctc ccttattacc ctcgaggaag aaaccaaaag caaggcggat
    14341 caatggcatg gccacggtta ctaacaaaag aaaacctgga gcaatggcat cagtcgggat
    14401 ttttgactgg cgaaacattt tttgactatc tgaaaaaaga cgttgagttg ttttattaca
    14461 agaaaatcat cgaagaatat catttgccga ttccaaagaa aaattttgag catgattttc
    14521 tctccctctc ttcagaggag tgtctcggaa agcatccagc actaatccct tatagatggg
    14581 attggtcgta tttagaaatt cctcttacct atcaggatga gtcctttgca gaagctagcc
    14641 gagcatttat tgaagggcag ataaaagaag cggaaaaagg caattgcaca ggagcatttg
    14701 cttcaacatt tgatgcatta aaagactggc gtgatccaat ccggcaggct attgaatggc
    14761 acgttttttc tgcaaaggaa tacaaagaaa tactatgggg atggtttact catctaaata
    14821 gctttttaac gatcggtccg ccagtcattc ggacgaaaga attagcagca ctgatagatg
    14881 cgggtatttt tcatttgttc gaaccgccat ttgatattca gcaaaagcac aaccgctatg
    14941 ttatttctct acaagataaa gaaatcagca gtcgctattt gattgaagct cgattgccac
    15001 aaacaagctt ttctcgttca agtaatcctg tcataaaaaa tttgaaggaa aacaaacttg
    15061 ttcgcccttt ttcattttca gataatacag gaagttatca ttcagaagct atagatgttg
    15121 agcgaggtac gaatcgcgtg attgatgctt caggggaaat ccagtctgac ttttattgct
    15181 atggaatacc tgttgaaggt gtggattggc taactgcagc agtagctaga ccttatacag
    15241 atccatggaa tctgagacag gcagataaaa tagcacaact tatattgact gacaaataaa
    15301 aatacgcatg atgacttatc tgttctttca caagtcttca tgcgtgtttt taaataatta
    15361 ttaacgagaa acgcgtttgc gcattgcttt tttacggttt tcttcgatca ttgcttcttt
    15421 ttcgtcaatt ggatcgatca ctgttttttt cacagaagcg accaaaccag caacggcagc
    15481 ggctgttgcc agacttccta ctaatattcc agatacaaat tttttcattg aatccacctc
    15541 atttattatt tgttatatct attatggaac aaaagagaaa aaaaggctag ttttacgttt
    15601 gtttgtaaac ctttgcaaaa aatccttgaa aatgtttact aaatgtttta ttataaattt
    15661 accatatgaa tttacgaaat tttactttgt ttgttttcac cataaaagta aacttcgttt
    15721 ttttaaatta tatataatca acaaacacaa ttttactaat caaaggagtg ttttttatga
    15781 caattttagt actaggcgga gccggttata tcggttcgca cgctgtagac cagctagttc
    15841 aaaaggggta tcaagtagct gtggtagata atttgctcac aggtcacaaa caagcagtac
    15901 atccagatgc tcatttttat gaaggagaca tccgggataa agaatttcta cgttccgtat
    15961 ttgaaaagga accgattgaa ggagtaatcc attttgctgc aagctcttta gtaggagaat
    16021 ctgtcgaaaa accattgatg tatttcaaca acaacgtata cggtatgcaa atcttacttg
    16081 aagtcatgca cgagttcaat gtgaataaaa tcgtgttctc ttctactgca gcaacttacg
    16141 gagaaccaaa agagtctcca atcacagaag atacaccagc taatcctaaa aatccgtatg
    16201 gtgaaagtaa attgatgatg gaaaaaatga tgaagtggtg tgaccaagca tatggcatgc
    16261 gctatgtcgc tttgcgctac ttcaatgtag ccggagcaaa agcagatgca tctatcggag
    16321 aagatcatac accagaaacg catcttgtcc caatcatttt acaagtagct cttggtcaaa
    16381 gaaaagcatt ggctgtttat ggagacgatt atgatacacc agatggaaca tgtatcagag
    16441 attatgttca agtagaagat ttgattgctg cacatatttt agctttggaa tacttgaaag
    16501 aaggaaatga aagcaacttc ttcaatttag gcagcagcaa aggatactca gtcaaggaaa
    16561 tgctagaagc agctagagaa gtgacaggaa aagaaattcc agcagaaata gcaccaagaa
    16621 gagctggaga tcctagccga ttagtcgctt caagtgaaaa agcgagagag gttctgggtt
    16681 ggaaacctga atacacagat atcaaagcaa ttatcaaaac agcatgggat tggcatgtca
    16741 gccatccaaa tggttatgaa gaatagggga atgagatatg tcaatcagtc aaacgatttg
    16801 tgattttgcc acacttgcga tcgaagctgg cggatggatg gaaatggacc gcttatattt
    16861 acagaatcgt cttttaggga tggtaggaga agatgctcta gaaagcattg aagtaagacc
    16921 agttaaggaa gaatctgtag acttattgga tcagcttgtc gctaaggcaa gagaaaatgg
    16981 tgttctttca gaattgccag cagaacaaga gatttttgaa gcgcaattaa tggattttct
    17041 cacaccgcca ccttcagtcg tcaatgcatt ctttgcacag cactattcga aaagcccaga
    17101 ggaagcaact gagtatttct ttaatttaag taagaaaaat gactacatca aaacaagagc
    17161 catcgcaaaa aacatcaaat ttccggcaga gtcggagtat ggtgaactgg aaatcacaat
    17221 caatttatct aagccagaaa aagatccaaa acaaatcgca gcagaacgcg aggcggtaaa
    17281 agtagactat cctacctgca tgctttgcat ggaaaacgaa ggatatagag gtcgcttgaa
    17341 ctatccagcc cgcaccaatc accggattat tcgaatgaat ctagacggtg aaagctgggg
    17401 attccaatat tctccttatg cttattacaa tgaacattcg atcattttat cagaacaaca
    17461 ccgcccgatg aaaatcacaa aggaaacatt tgctagactg cttcggatta ctgaagtctt
    17521 gcctcattac tttgttggtt cgaatgctga cttgccgatt gttggaggat cgattctttc
    17581 tcatgatcat tatcaggcag gacgtcatga atttcctatg gcaaaagcac cgatagaaaa
    17641 acttatcaaa ctagcagatt atccgcaagt catcgcagga gtcgtgaaat ggccgatgtc
    17701 tgtgatccgt ttgcaatcaa cagagaaaaa attgttgatt gaagcagcta actcagtatt
    17761 ggaaaagtgg aaagcatatt cggatgaaac agtatctatc agagcttttt cagcagatgg
    17821 tacaccacac catacgatta cgccaattgc acgaagaagg gacaattact ttgagttgga
    17881 ccttgtcctt cgagacaaca atgtttcaga agaacatcct gatgggattt ttcatccaca
    17941 taaagatgtc caacatatca aaaaggagaa cattgggttg atcgaagtaa tggggcttgc
    18001 tgttttacca cctcgtttga aaccagaact agtagaagta gagcatttct tattaggaga
    18061 agaaaaccag atggctgaat atcatcgtcc gtgggcagaa caattaaaaa aagaacatcc
    18121 tgatttgacg aaagatacgg tcaccgatgt cgtacaaaaa tctgtaggtc aggtttttgc
    18181 acgtgtactg gaagacgctg gagtattcaa gcgagatgaa aaaggacagg cagcgtttga
    18241 acgatttact gcaattttat aaacgtaaaa aaggaggaga gacaaatggc aacaattaaa
    18301 gacattgcgc agttggcagg agtttctcct gctactgttt caagagttct caactatgat
    18361 cctgatcttt ccgttggctt agccacaaag caaaaaattt ttgaagcagc agaacgctta
    18421 aattatacga aacatcgtaa aaatgcagca aatactaagc aaacgcttcg cctgatccag
    18481 tggtacgacg atacggaaga gcttgaagat ctttattatt tatctattcg tctaggaatc
    18541 gaaaaaaagg cagaagaagc aaacgttact ttattgaaag aaacgtggga ttccattagt
    18601 gatcttccga cagatggaac gatcgcacta ggaaaattcg acgaagctca gttattagaa
    18661 atgaaaggat cccaaaatgc cttgcttttg gtagattctg acggcacttc tcaaggaatc
    18721 gactctctag ttgtcgactt taagtcaagt gttcaaaaag tcattgaaca ttttgtctcc
    18781 cataaagcgc aacatatcgc tatgcttgct ggaacagaat atacgaagaa aaaccatcag
    18841 cggctaaaag atcccagaat tatggcattt caacaagttt tggcagaaaa aggctacgac
    18901 gaaggtccaa ttatcgaagc agatttttct gtcgagtcag gatatcaagc agtgaagcag
    18961 tatttaaaaa cgaatccgca tgttcctgat gcgctttttg cagctaatga tgcattagcg
    19021 attggcgcac tgaaagcgat tcaagaaaaa ggtttagtgg taccagaaga tatttctgtg
    19081 atgggtttca atgatgtcag tgtagcgaaa tatgtaacgc cctctctttc tactgtaaaa
    19141 gtttataccg aatggatggg agagctagca gtggagacga ttcttcaatt aagtaattcc
    19201 aaagcaccgg tttcaagaaa gattattgtg gaaacagagt tgattattcg agaatcaacg
    19261 aaataaaagg gctctttgtc aataaggact gatgagttgt gcaaaatcaa atctgagtca
    19321 gaatgaacca
//
LOCUS       DAZAFF010000052        18555 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000052 DAZAFF010000000
VERSION     DAZAFF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.052"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..797
                     /locus_tag="ACG9BB_001729"
     CDS             150..797
                     /locus_tag="ACG9BB_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFN2837669.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    926..1142
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1190..3835
                     /locus_tag="ACG9BB_001730"
     CDS             1190..3835
                     /locus_tag="ACG9BB_001730"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFN2837670.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4145..5458
                     /locus_tag="ACG9BB_001731"
     CDS             4145..5458
                     /locus_tag="ACG9BB_001731"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFN2837671.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTEAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5448..6101
                     /locus_tag="ACG9BB_001732"
     CDS             5448..6101
                     /locus_tag="ACG9BB_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN2837672.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6156..6848
                     /gene="radC"
                     /locus_tag="ACG9BB_001733"
     CDS             6156..6848
                     /gene="radC"
                     /locus_tag="ACG9BB_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFN2837673.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6998..7198
                     /locus_tag="ACG9BB_001734"
     CDS             6998..7198
                     /locus_tag="ACG9BB_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN2837674.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7452..9131)
                     /locus_tag="ACG9BB_001735"
     CDS             complement(7452..9131)
                     /locus_tag="ACG9BB_001735"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFN2837675.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9133..9345)
                     /locus_tag="ACG9BB_001736"
     CDS             complement(9133..9345)
                     /locus_tag="ACG9BB_001736"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFN2837676.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9738..11468
                     /gene="efrA"
                     /locus_tag="ACG9BB_001737"
     CDS             9738..11468
                     /gene="efrA"
                     /locus_tag="ACG9BB_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFN2837677.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11465..13225
                     /gene="efrB"
                     /locus_tag="ACG9BB_001738"
     CDS             11465..13225
                     /gene="efrB"
                     /locus_tag="ACG9BB_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFN2837678.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13320..13517
                     /locus_tag="ACG9BB_001739"
     CDS             13320..13517
                     /locus_tag="ACG9BB_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837679.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13670..14155
                     /locus_tag="ACG9BB_001740"
     CDS             13670..14155
                     /locus_tag="ACG9BB_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFN2837680.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14259..14852)
                     /gene="clpP"
                     /locus_tag="ACG9BB_001741"
     CDS             complement(14259..14852)
                     /gene="clpP"
                     /locus_tag="ACG9BB_001741"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFN2837681.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            15032..15559
                     /locus_tag="ACG9BB_001742"
     CDS             15032..15559
                     /locus_tag="ACG9BB_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837682.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15650..16387
                     /locus_tag="ACG9BB_001743"
     CDS             15650..16387
                     /locus_tag="ACG9BB_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFN2837683.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16391..17308
                     /gene="miaA"
                     /locus_tag="ACG9BB_001744"
     CDS             16391..17308
                     /gene="miaA"
                     /locus_tag="ACG9BB_001744"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFN2837684.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17310..18539
                     /gene="hflX"
                     /locus_tag="ACG9BB_001745"
     CDS             17310..18539
                     /gene="hflX"
                     /locus_tag="ACG9BB_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFN2837685.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5995 a         3210 c         4033 g         5317 t
ORIGIN      
        1 tacgtttggt tgagcttatt accgcagatg caagcttttg aacaccgttt tatttgtctc
       61 acaccctttt ccttcctact tgtaaccttg ctcccgtgtt agaaacatgt tacactaaat
      121 atgtgaaatt attaacaagg agcgtcgtta tgcgagaaaa aacagataca ctgccaaaag
      181 caactgcgaa aagattacct ttatatttac gctacttgaa gttgctggat gactcaggta
      241 taagtagaat caaatcaaat gagttcagcg aaatcaccca aattccttct gcaacgatcc
      301 gcagagactt ctcgcaatta ggcgagctag gcagaagcgg atatggctat gatgtccctt
      361 ttcttatcga tgtattcaat aatatcctta atactaaaga agaaaaacga atcgcattag
      421 taggatatgg aaatcttggc aaagcattga aacacaacaa ttttagaaga aatgaaaatc
      481 taaatatcgt gtgcgtcttt gataatgatc cagcactgat caatcgagta attgatggag
      541 aaatgatcta tccgattgat cgtttcgcgg agatagccaa agcaaaaaat gtgacagtag
      601 caatctctac ggtaccaagt aaatattcac aatctgctat tgatgaaatt gtcaaaggaa
      661 atgtaaccgc tatacttaat tttgcgcctg acagagtaac tgtcccagct tatgtcaatg
      721 ttcaatacat tgatttgaca accgagttgc agacgcttat ttactttgat gagaattact
      781 cagaagtttt ttcttaggaa aatttgagta ggcttattga tcttgcgaga actcgtcgta
      841 attctttcaa aaatttttta gtactaaatg aagtggtttt ttcacgacca cttgttgact
      901 aaaaaaaaaa accattgtag aatgaagcat aacgctgaac aagaggagta actggcacat
      961 tcattcttag agagagaact atctgctgaa aggttctcaa tgacaaccag tgaaggtagc
     1021 ttgggagttt ctgtcttgaa tggagtagag atagacgagt cgtgatcgtt aactcacttg
     1081 agaggcaata atgattgatt attgcaattt aggtggtacc gcgtaaatca tacgtcctag
     1141 aatacgaaag tattttagga cgttttttag tttaaaggag gaaaacagca tgacggaaga
     1201 aaataaccta tcaacaaaat ataatccgca agaagttgaa gctggacgtt atcagcattg
     1261 gttagaacaa gatttgttta aaccgagcgg agataaaaaa gcaaaacctt attcaatcgt
     1321 aatcccgccc ccaaatgtta ctggaaaact tcatttaggg catgcttggg atacgacctt
     1381 gcaagatatg ctgattcgtc aaaagagaat gcaaggatat gatactttat ggctgcctgg
     1441 tatggaccat gcaggaatcg cgacacaagc aaaagtagaa gcgaagctta gagagcaagg
     1501 gatttctcgt tatgatctag gtcgtgaaaa gttcatcgat caagtgtggg aatggaaaga
     1561 agagtatgcg agccacatcc gcgaacagtg ggctaaaatg ggcttatctt tagattacag
     1621 tagggaacgt tttacattag atgatggatt atctgaagca gtacgtaaag tttttgttac
     1681 cttatacgaa aaaggtttga tttatcgtgg tgaatatatc atcaactggg atcctcaagc
     1741 acgaacagct ctatctgata tcgaagttat tcataaagat atcgaaggcg ccttttatca
     1801 tatgagctat gaactagcag atggatcagg cgtagtagaa attgcgacaa cccgtccaga
     1861 aacgatgttg ggagatacgg cgattgctgt tcatccagaa gacgagcgtt atcaagcgct
     1921 catcggcaaa aaagtgattc tacctttagt caataaacaa atcccaatca ttgcagatga
     1981 atatgtagat atggaattcg ggacaggtgt agtaaaaatc actccagcac atgatccaaa
     2041 cgactttgag gtaggtaatc gtcatgatct tccacgcgta aatgttatgg atgatgatgg
     2101 tacgatgaat gaactagcag gaaaatatgc tggaatggaa cgttttgctg ctcgtaaagc
     2161 aatcgtgcaa gatctgaaag aaatcggtcg attgattaaa atcgatacga tgatccacag
     2221 tgtgggtcat tccgagcgga caggtgtcgt tgttgaacct cggctgtcta ctcaatggtt
     2281 tgtaaaaatg gcgccgttag cgaaaaaagc cattgataac caagaaacag atcaagcagt
     2341 ggaattcttc cctccaagat tcaatcaaac gtttttacgt tggatggaaa atgtccatga
     2401 ttgggtaatc tcacgacaat tatggtgggg acatcaaatt cctgcatggt atcacaaaga
     2461 aacaggtgaa atgtatgtag ggatggaagc accagcggat agtgaaaact gggtacaaga
     2521 tcctgatgtt ttagacactt ggtttagttc agctttgtgg cctttctcaa caatgggctg
     2581 gccagatacg gaaagcgaag attataagcg atacttccct actagcactt tagtaaccgg
     2641 ttatgacatc attttcttct gggtaagcag aatgattttc caaagcttgg aatttactgg
     2701 cgaacgtcca tttgagaatg tcttgattca tggattgatt cgtgacgaac aagggcggaa
     2761 gatgagtaaa tcattaggta atggaatcga tccgatggaa gtaatcgaaa agtatggagc
     2821 agatgctctc cgctggttct tatcgaatgg ttctgcacca ggacaagatg tccgcttcag
     2881 ctatgaaaaa atggatgctt cttggaactt tatcaacaaa atctggaatg cttcacgttt
     2941 cgttatcatg aatatcgaag gaatgaccgt agaagagatc gatttatctg gagaaaaatc
     3001 agtggctgac cggtggatcc ttactcgttt gaatgaaacg atcgaacgag taactgagct
     3061 atttgatcgt tttgaatttg gtgaagcagg tcgacaactg tataacttta tctgggatga
     3121 tttttgtgac tggtacatcg aaatgagtaa agaagtactt tatggagatg atgctgtttc
     3181 aaaacaaacc acccgtagca tcttagtcca tacacttgat cagatcctac gtttattgca
     3241 tccaatcatg ccattcgtta cagaagaaat atgggaacat attcctcatc agggaaattc
     3301 tctagtagtg gcagaatatc ctgttgtacg tccagagttt gatgatgaaa cagcttctaa
     3361 agggatggaa gtattgaaag aattgattcg agccgttcga aatattcgtt cagaagtgaa
     3421 tacaccattg tctaaaccaa ttacgttgct aattaaaaca aacgatgcta aaatcgatca
     3481 atttttagtt gaaaatacaa gctatatcga acgcttctgt aatcccgaag agttgacgat
     3541 atcaagtgag atcactgcgc cagatctagc gatgtcagct gttctgacag gtgcagaaat
     3601 cttcttgccg ttagcaggat tgatcaatat tgaagaagaa atcaaacgat tagaaaaaga
     3661 actggaaaag tggacaagcg aagtaaaacg tgtccaagga aaactttcta atgaacgttt
     3721 tgtttctaac gcgccggaag aagtagtaga ggcagagcgt gcaaaagaaa aagattattt
     3781 agagaagcaa acggctgtca aagaacgaat cgaaagtttg cgtactattc aataagaata
     3841 gaaaaagaga gtgtgaaatc agctagctat tactaaaagc tagctgattg aacatcgtta
     3901 caggaaagag tttgagcctc actcttagct tcgattcata aggagatgtg acataaattt
     3961 tgtcgcatct cttttttagt ctactcttat tatgtaggag aaggagggag gtggatcatg
     4021 tatcaagagt caataaagta gttgataaaa tcgttcctct ctctgtttct tttctctttg
     4081 tcatactttt atttttctta tgtgttctat ttgttataat aaaagagaaa tgggggaatt
     4141 gttgatgacg attgaagaag caatagaatg gatccatagt cgattaccat ttggttcacg
     4201 tcctggcctt gaccgagtcg aagctttatt agaagtggta gggcatccag agagaaaagt
     4261 aaaaacgatc catatcgctg gaacaaacgg aaaaggttct actgaagctt atttgagaag
     4321 cctattagaa gaggctgggc tgacagtagg tacatttact tcaccttata tcgaaacgtt
     4381 caatgaacgg atcgcaatca atggcaaaag cattccagac aatgatttga tccagcttgt
     4441 aaaaaaatat cagccactga tcaaacaact tgatcaattt gaagcagtag ctggtattac
     4501 tgaattcgag acattaacag caatggcatt tgattatttt ttgatgaaga aagtggacgt
     4561 agcgattatc gaagttggct taggcggctt attggattct accaatgtag ctgtaccaat
     4621 gctgacggga ataacaacga ttggacttga tcataccgaa atacttggag atacaattga
     4681 agaaatcgct gcacaaaaag ctggaatcat caaacaaggc gttccagtag taacaggaaa
     4741 tattgcccca gaagctttta ctgtcatcca acaaacagct aaagcaaaaa atgcaccgct
     4801 ttatgtatac ggggaaaatt ataaaataaa ctatctgcat cctgatgaga tgtggggaga
     4861 agtatttgaa ttttttaatg aaataggaaa gatccctcaa ttaaccactc cgcttttagg
     4921 gcaacatcaa gctgaaaatg caggagtagc catcgaattg tattttgttt actgtcaatt
     4981 ggaaaagctg cctttccgag aaaaagatgt tttgaaggga ttgaagaaat ctcgatggcc
     5041 aggaagaatg gagcgtttga gtaacgagcc attagttgtt ttagatggtg cccataatac
     5101 gcatgcaatg aaccggcttg ttgagaattt gaaaaaagaa ttcaaaggat atcaaatcaa
     5161 cattttgttt tccgccttac gaactaaaaa tgttagagaa atgctagaac gattgcagca
     5221 agtaccaaat gtccatattt atttgacgac ctttgaacat ccaaaagcca tcgaattaag
     5281 tgaagtatca gatttagcaa gtgagagagt atcaatcgtt tcattgtggc aattcggatt
     5341 agcagatata ttggaaaaga tgacaaatga agacgtatta gttgtgaccg gttcacttta
     5401 tttcatttca gaagttcgcc atttattaat acaattagga ggagatcatg aagtataatg
     5461 cagtgatttt tgacatggat ggtttgctgt tcgacacaga gttagtgtat tacgaagcca
     5521 cacaagtagt agcagacaag atgggttttc cttatgataa agacctgtat ttaaaattct
     5581 taggtgtttc agatgaagaa gtatgggcga attatcatat gattttctcg gaatttggca
     5641 aaacaagtgt acaaagattc atagatgatt cataccagga aacgcttgaa cgtttttcgc
     5701 aaggagcggt acaaatgaag cccggtgtgt tggaactcct ctctttttta gaagaacatc
     5761 agattcccaa agttgtcgct tccagtaacc aacgagcagt cattgaactt ttattgaaga
     5821 agaacaatct gtttgaacga tttgagacga ttgtttctgc agaaaatgtt caacgagcaa
     5881 aaccagaccc agaaattttc ttgttagcta atcagaagct aggtacagcg aagaaagaaa
     5941 cattgatttt agaagattca caaaatggta ttttagcagc ttctgcggct gagatcccgg
     6001 tagtcatggt accagatctg cttccaccat cccaagaatt gatcaaaaaa acagctgggg
     6061 ttgtttcttc tttacatgaa atccctgcat tgttaaaata aaaatccatc tcttttcgta
     6121 tcttatttta gataagatag aaaggagatg tttttttggg aaaacgattg atcaaagaag
     6181 tacctactag ctctttacca agggaacgta tggaaattta tggcgcggaa gctttatcgg
     6241 atcaggagct tttggcaatc ttactgcgta ccggtcaaca cccttatagt gtgatgtcga
     6301 ttgctggaaa tctgttaaaa acgtttggag gactggcctc tttacgacag gctactttgc
     6361 atgagttaga agagattcga gggataggac gcgtgaaagc aatcgaaatc aaagccttga
     6421 tcgaattagg tcgacgaatt cagacggatc atgaaagagc ggctccaccg gttcgtacca
     6481 gctacgaatt ggcccagcaa ttgattctgg aaatgaaaga tcacaaacaa gaacatttgg
     6541 tttgtattta tttggacacg aagaatcaag tgatcttaaa aaaaacagtg ttcatcggtt
     6601 cgctgaatca gtcagtcgct catcctagag aaattttcca ttatgctgta cgttattgtg
     6661 cagcaaggat cgttttagcg cataatcatc cttctggtaa tgttattcca tcgcagcagg
     6721 atatgaattt taccaaaagg atacaaaagt gtggagaaat gatggggatc actgtactgg
     6781 accacttgat catcggacgc aaacgatatt ttagtttgag agaagaagga atgatggaag
     6841 agaagtaagt tgctttcttg caaaaaaaat gaagcaagtg tacaattata ttgtaatttt
     6901 gtttgggaat agaagttgaa acatctgttg cacctctatc gtacacgagc aatgttacaa
     6961 tttaaattag tgcttttggc acgaggagga tattattatg gaaaacggaa cagtaaaatg
     7021 gttcaacgct gaaaaaggat ttggatttat ctcacgtgaa gatggaagcg acgtattcgt
     7081 tcacttctca gctatccaag gtgacggatt caaaacatta gaagaaggtc aagcagtgac
     7141 tttcgacgtt gaagaatcag accgcggccc acaagctgca aacgttgtaa aagcttaatt
     7201 ttatctgtaa atcaaattga tggtagacat caatttttac gagagaacaa agcgagtaac
     7261 aaaaatacct gccacaagag acaatttctc ttgtggcagg tatttttttg tatgaaaaag
     7321 taaataaatt taagaggcta tgaaagagcc accttgtatc aaggaataag aacaaacaaa
     7381 aatggcttct catgtttttc atttgttttc tcttatattc aggacaaggt gctgatttca
     7441 taacctctta tttattcttc atctggtgtc attaccattg gcaaaatcat tggatgtctt
     7501 tctgtacgtt cgaataagaa aggctgaagc gcgccagtca tcgcttcttt caatttatat
     7561 tctgaacagt caggtccatt caaacattct cttaaagcgt ggaataagat tcgctgagct
     7621 tcattgatca tttctcctga ttctcgcatg tagacaaatc cacgagagag aatatctgga
     7681 ccagccaaga tttctttttt cttgatatcc actgtcgcga cagctaatac aagaccttct
     7741 tctgaaagca ggtgacggtc acgtaaaacg acattaccaa tatcaccgat cccgctacca
     7801 tcgatataca catctccagc cacaaaatgc ccagcaggac gcgcagaatc gctagttaac
     7861 gctaaaacat cgccattttc catgatgaag cagttttctg caggaacacc tacatcttgt
     7921 gctagagaag catgtacttt caacatgcgg aattctccgt ggacaggaat aaaatatttt
     7981 ggcttcatca aacgaagcat taatttttgt tcttcttgtc caccgtgacc ggaagtatgg
     8041 atgttattga tttttccgtg aatcacttct gcaccagctt cagacagcaa attgatcaaa
     8101 tgattgacac ttgttgtatt accaggaata ggtgaacttg aaaagatcac tgtatcgtct
     8161 ggctggattt ggatttgacg gtgagtacca ttcgcgatgc ggctcaacgc ggccatcgct
     8221 tctccttgag aacctgtaca taggatcatc gtttcattgg caggcaagcg attgatttcg
     8281 tgtgcatcta caaatgtgcc gtctggaact ttgatatatc ctaaacgttg accgttgacg
     8341 atggcatttt ccatgcttcg tccgaaaaca gcgattttac gaccagttgc cacagcggca
     8401 tctgctgctt gctgcaaacg gaagatgtta gaagcaaagc tggcaaaaat gatacgtcct
     8461 tcgatttttg aaaagatctt caaaatagaa tgcccaatcg ttttttctga tttagtaaat
     8521 gttggaatct cagcatttgt actatcagat aaaaggcaca agactccttc ttccccgata
     8581 cgagccattt tatgcaagtt tgctggttcg ccaacaggag tgaaatcgaa tttgaagtct
     8641 ccagtcgcta cgatatttcc agaaggtgtt ttcaccacga tccctaatgc atctgggata
     8701 ctatgggtcg ttctgaaaaa gctgacagaa gtctttctaa aacggatgat cgtatcttca
     8761 ttgatctcat ggagctctgc atctcgaagc aagccatgct catctaattt gtttgtgatc
     8821 aacgctaatg cgagaggacc agcatagata ggaatatttg cttgtttcag caagtagggt
     8881 attccgccga tgtggtcttc gtgcccgtga gtaatcacca aggcttttac tttgtgtaag
     8941 ttttgtatga tgtagctgta gtcgggaatg acatagtcaa ttccaagtaa atcgtcttcc
     9001 gggaatttga tcccggcatc aatcaaaatg atttcatctt ggaattgtac cccatacgta
     9061 tttttcccga tttcacctaa tccaccaata gcaaagacgc ctgtctcgtt atttttgatg
     9121 gaaagtttca tattaaaact ccactaatga gaaatcagca ttttcttttt cgtactctag
     9181 atgacttcct tctagaagtt gaacgaattc aatattgtat ggggtatttt cttccacacg
     9241 ttggcgagcg tctacatcgc tttttgcctc aatgtaaaga gattttgttt cttcccgttt
     9301 agggttgcgt acttttgttt cttgataata aactttatag atcattgttt tgtctccttt
     9361 atctgcgtaa agcagtgatt tagctagttg atttttttag acaacacaaa acgaggggac
     9421 aagccacctg gtttttcata tgctttgact agaatactgg attcgagtca aagagacaag
     9481 cctcgttttt gcttatctgt tcggatattt tttcaaccgt accttctgta taaaaaatat
     9541 acaacaatta attgttattc tagcataaaa cagcaaataa gtatagaaat caactagatt
     9601 gaaagaaaaa atcaatgttt atcatcccgc tataatcata atgacttttt tatgagagaa
     9661 gtgttctatt aataggctcg ttgtgctatc atttttagta atgaaaatgt aacaaaagat
     9721 taaaaggaga tgagaaagtg aaactaatgt ggcattatac catgcgttat aagaaattct
     9781 tgtttttcaa tttcatatgc gtatttggtt ttattttgat tgaattagga cttcctacga
     9841 ttttagcacg tatgatcgat gtaggaatcc gcaatgatga ttttgattat gtgaagcaac
     9901 aagggatatt tatgatcgtt attactgtaa tcggtattat tttgaatatc ttgttaggtt
     9961 attttggttc gaggattacg actaatatcg tagcagatat tcgagatgat ttgttcgaaa
    10021 agatccaaag ttattcgcat caggaatacg aaaatctagg cgtatcttca ttgatcactc
    10081 gtacaacaaa cgatgcttat cagatcatgt tgttcctgca aaatatttta cgtatcggtt
    10141 ttatggcacc aatgatgttc attgtcagtt tgtacatggt catgcgtaca agtgtgacac
    10201 tcagtttgta tgtagtaggt gcattacctt tactattatt ggcagtcgta gcgattgcta
    10261 aggtgtctga accgttatct aaaaagcaac aaaaaaactt agataaaatc aacagtatct
    10321 tgcgggaaaa tctttcagga ttgcgtgtga ttcgtgcatt cgtcaatgaa aagtttgaag
    10381 aaaaacgttt tgctgtagtc aatgagaatt atgcttctag ttcaaaaagc ttattccgtt
    10441 tgatggctgt tgcacaaccg ggtttttatt tcttatttaa tatcgtaatg gtattgatca
    10501 tctggagcgg ggcagtgcaa atcgatgcag gcgagcttcg tgtaggagat ttgattgcgt
    10561 ttatcgagta tatattccat gccttgttct cattcatgtt atttgctagt gtgttcatga
    10621 tgtatccaag agcagctgtt tcagctggac gtatccaaga agccctagat gccaaacctt
    10681 cgattgtaga agatccaaat ggtatatctg atacaaaaac taaagggtat atcgaatttc
    10741 aaaatgtaac atttgcttat ccaggccatt cgcaagagcc agtgatacga aatgtcagtt
    10801 ttacagcctc tccaggagag accgttgctt ttattggaag taccggtagc ggaaaatcta
    10861 cgttgatcca attgatccct cgcttttatg atgtgagtga aggaaagatc ttgatcgatg
    10921 gagtagacgt gagagattat cggctaagca agttgcgaga taagattggg tttattccgc
    10981 aaaaagcgct gctatttact ggaacgattg cagataattt gcgatacggg aaagaagatg
    11041 ctacgcaaga agaaatggaa cgtgcagctg agattgctca agcttcagaa ttcatttctc
    11101 gaaaaccaga tggatttaat gaacatttat cagaaggtgg ttccaatttt tcaggtggac
    11161 aaaaacaacg attggcaata gccagagcca tcatacgccg tccggaaatc tatatttttg
    11221 atgacagctt ttctgcatta gattatcaaa cggatgcgaa gttacgtgct cgtctgaaaa
    11281 aagaaacgac agaatctact gtattgatcg tagcacaacg tgtgggaacg atcatgcatg
    11341 cagataagat catcgtatta aatgaaggag aagtcgtcgg tatggggacg catcgggagt
    11401 tgttggaaaa atgcccgatt tattatgata ttgccgcttc tcaattatca aaggaggaat
    11461 tagcatgaaa aaaacctttt cttccttaaa acgtttagct cgatatatca gaccgtatcg
    11521 tgagactttt cttttagtga tcctatttac agttctgaca gtggctttca acgtggcttt
    11581 gccatatgtt acaggacttc ctactacaga aatcagccgt aatattgcca atggcgaatc
    11641 aatcaatttt tcttatatat tcaaatgtct catttggatc acagttgttg gaatagggta
    11701 ttgtatttcg caattacttt ctggtgtttt gatgacaaat gtcgttcaaa gtgccatgca
    11761 tgatttgaga agagacatag aagaaaaaat caatcgtctt ccggtttcat atttcgataa
    11821 aaaccagcaa ggaaatattt tgtctcgtgt gacgaatgat gtagatgcag tcagcggtgc
    11881 tttacaacaa gcttttattg gtatcgtaaa tgcgatattg ggaattacgt tggctgcagc
    11941 aatgatgttt tatatccagc cgatgatggc gctgatctct atgatcatga ttccaacatc
    12001 gatctggatc tcaaaacgag tgatcaatgc ttcgcagaaa tattttcaaa gcatgcagaa
    12061 ttctcttggt gagctaaacg gctacgtcca agaaaatatg acaggtttta gtgtattgaa
    12121 agtatatgga cgcgaaaaag aaacattgga aggattcaaa aaagttaatc acagcttgaa
    12181 atactttggg tttcgtgcag cattcatttc tggcttgatg atgcctctag ttcaattaac
    12241 tgcctatgct acttatattg gtatggctgt tctaggaagc ttttatgtta ttactggggc
    12301 gatcgttgtc ggtcaactac aagcattcat ccaatatatt tggcaaatca gtcagccaat
    12361 ggggaacgtg acacaattgt cttcagtttt gcaaagtgca tcagcggcaa cgaaacgtgt
    12421 attcgagatt ttagatgagc cagaagaaaa agaaaacaaa gtagatgctt cactgccaga
    12481 aacgatttta ggagatgtga cattcgatca tgtcgatttt gcatatgatc cgaagaaacc
    12541 attaatccaa gatttgaatt ttgaagtgaa agctggtcaa accgtagctg tggtcggtcc
    12601 gaccggtgcc ggcaagacga cattgatcaa tctattgatg cgtttttatg acgtagacaa
    12661 aggagcaata aggattgatg gaatcgacac caaatcaatg agtcgcagtg atgtgcgttc
    12721 gctatttggt atggtcttgc aagatgcatg gctttatcaa ggaacgattg cagataatat
    12781 ccgcttcgga aagcttgatg cgacagatta tgaagtagtt gatgctgcga aaacagcaaa
    12841 cgtagatcat tttatccgaa cgatgccaga tggatatgat atggccatca attctgaggg
    12901 agacaatgtg tctttaggac aaaaacagtt gctgacgatt gcccgagcag tggtctccga
    12961 tcctaaaatc ttgattttag atgaagcaac aagttcagta gacacccgtt tagaagcgtt
    13021 gattcaaaaa gcaatggatc gcgtgatgga gggacggaca agtttcgtca tcgctcaccg
    13081 tttatcgaca atccgtgatg cagatctgat tctagtgatg gatcaaggtc agatcattga
    13141 aaaaggaacg catgagagtt tattggcaca aggtggattt tatgaaaaac tgtataacag
    13201 ccaatttgct gaggaagcag aataagtcat ataagaataa aacagtttaa aacttatttt
    13261 ttgttctcta ttcttgataa tggtaaagta aagttatcaa gaaaagtgag gacacggaga
    13321 tggaacagtt tttaaatgaa aaaattactg aagccaaaag agaagttgat cgtttaacgc
    13381 acgtctgtca gacgaagcct tttgattcag ctgatttttt tgaaaatgag tttcaaatcg
    13441 aacgcttggc agcacaaata gaagtgtacg aattaatttt aaaaaaagta atagaagaaa
    13501 atatagatgt acaataaaaa caggtaaaac gtcagttacc agatactgta aatagacatc
    13561 tggcctgagc aggttttacc tgtttttttt cttatgaaat aaatatatgc tatgatacct
    13621 ttattcaata ggattagttt ctacaaaaaa tgaaaggaga aaaataaata tggaacggtt
    13681 tatagcatca aatgccacag tgattggtga tgtgacattg tcagaagatg tgacgatctg
    13741 gtatcaagct gttttacgcg gggacagtaa ttggataaaa ataggacaaa gaacgaatat
    13801 ccaagatggt acaatcatcc atgtcgatca tgatgcacct gtagatattg cggagaatgt
    13861 gacggtagga catcagtgca tgctacatgg ctgtacaatc gaaaaagggg cgctgatcgg
    13921 gatggggacc acgatattga atcatgcggt tattggtgag aatagtttga ttggtgctgg
    13981 atcattagta acagaaggaa aagttattcc tcctaatgtc ttggcttttg gtcgcccagc
    14041 taaagtgatc cgtcctttga caaaagaaga aatacaaaaa aacaaagaaa acatccagca
    14101 ttatgtcgag ataggtcgga tgcatgcgca aggaaaattc aaggagcaaa gataggcagt
    14161 agcatccata aaataaaaaa ggatggtcag tttgagttga ccatcctttt ttatttggtt
    14221 tagataagag agcttaaaaa agcttccttt gaaacagatt aatttaatga agaactgttt
    14281 tccatgactt catcgatcaa tccatatgct tttgcttgtt cagctgtcat atagttgtca
    14341 cgatctgtat ctttttcaat cacttcaagc ggttgtccag tgcgttcagc taaaattttg
    14401 tttaagcgtt cgcgtgtttg caaaatatgg cgtgccgcaa tttcgatttc agttgcttgc
    14461 ccttgagcgc cgccaagagg ttgatggatc atgatctcag catttggtaa agcaaatcgt
    14521 ttaccttttg taccagctgt cagcaagaag cttcccattg aagcagccat acccataaca
    14581 attgtttgga catctgcttt gacaaagttc attgtgtcgt aaatagccat acctgctgta
    14641 actgaaccac caggagaatt gatataaaga tagatatctt tttcagaatc ttgagcgtct
    14701 aagaaaagca attgggcgat gatcgaatta gcaagatcat ctgttacttg gccgcttaac
    14761 atgatgatac ggtctttcaa taaacgtgag tagatatcgt aagctctttc acctcgtgaa
    14821 gattgttcaa tgactgtagg aattaaattc ataatagagt tcctccttga tttcgtttag
    14881 aaatagtgta ccataattta gtttctaata cctaaaatgt agtatacatt tttggtcaaa
    14941 aaaggtcaaa taaaaaactt aggcaaatta gtcgcataaa cccgctttat ggtatgataa
    15001 gcagagaaac aaaacaagga ggaacaacag attgatagtt ggtattactg gccttatcgg
    15061 agtgctgaca ggtgctgtag ttgtctatat cggcatgata ctgcaaatgc gtcacttgga
    15121 aaataaagaa cagcgtagac gtgaattgga attgaaagtc aaagaaatcg agacgctgaa
    15181 tcaattaaat aaaaagatca acgagatctt gcaaaaacgg acaacgctga tggaagaata
    15241 tgtcagcttt gatgcatttg atgattgtta tatcacgatt gacgattttg cgtatctcca
    15301 atcattttca gcgcaaaata gtttttatct gccaaattat attttagacg agctatttaa
    15361 gaatatctcc catcgaaaag tgattttatc tccagatgaa acaatgaaga ttggtggata
    15421 tgcttataaa ggcggacgcg tgattttaga gaattttacg gatagtctga ctgaaatgat
    15481 caatgaaaaa aaagctcagt tgaagactat gacgcgccag ccgattggtt tcttctcgaa
    15541 agatgatgtt tggagctgat attttgaagc attgccgttg cacatgagag cgtggtaatg
    15601 ctttcttttt ttcttaatga tacaatagaa ctgaacggag tgaagaaaga tgttacaaat
    15661 ctatgcccat cgcggaagca gcggcaccca cccagaaaat acgcttcctg catttgcaga
    15721 agctgtacga gtaggtgcag atggtatcga actagatgtc catctaagca aagatggtta
    15781 tttgatcgtt atgcacgacg aagaagtgga ccggacgact aacggtaaag ggctgattcg
    15841 agaaaaaaca ttggaagaac tcaaaaaatt gaatgcaggg ggcaaattca gcaaagaatt
    15901 tccagcagct aaagttcctg aattgagaga agtgttagga ctgctcctcc aaaaaaatta
    15961 ccgagggatg ctaatgattg aaattaaaac tgatcagtat gaatatcccg gtatcgaaga
    16021 aaaattagcc gaaacattaa aaagtagaga ttggcctttt caacatgcat attgcagctt
    16081 tcatttggat tctttggaaa aaatcgcatt actggaaccg gagatacagc tggacttatt
    16141 gatgagtact tctgataaaa agatccaact agcagcagaa accccttata tagaaggagt
    16201 acatccaaaa aatacttggt tgttcgaaca tccagtccag gcagctgatt ttccaaaagc
    16261 aattcgtgca tggacgatta acgatgaaga agaaatggcc aaatgtcaag aactcaaaat
    16321 agatggaata ataactgatt ttccggagaa agctcttcgg tggaaacaat caacgaaaag
    16381 taggtaaaat atggaaaaag tattagtgat cgtcggtccg acagcagtcg gtaaaacagc
    16441 gctaagtgtg gaactcgcac aactatttgg aggagagatc atcagtggtg attcattgca
    16501 aatctataaa aatctggata tcggtacagc aaaaatcaaa gaagaagaga tgaataatgt
    16561 tccgcatcat ttgattgatg tgatcgaacc tactcaacgt tactcagccg ctgattttca
    16621 gcattcaggt cgacaattga ttaccgagat tgcgaaccga gggcacctac ctatcatagc
    16681 tggtggtacg ggtctctata tccaatccct tctatatgat tatcagttag gagcgacaga
    16741 agaagcgaat cctgagatta gaaaaaagta tgaaacgtta gctgaaacaa ttggttccca
    16801 agcattatgg aatcagttga aagaaaaaga cccggcagct gctgagaaaa tccactggaa
    16861 caatcaacgc aaagtgataa gagctctgga agttttagag acgacaggac atagtatcac
    16921 ggcacctaaa gaagagccaa agcaattgta tgattacctc atgattggat tagatacgaa
    16981 gcgtagtatt ctttatgaac gaatcaatca aagagtcgat ttgatgctag cagaaggttt
    17041 agtagcagaa gcgcgtaagg tgtatgagct tggagaagtc caggccagtc aaggtattgg
    17101 atataaagaa tttttccctt attttgaagg gaaagaaaca ttggaagaag caacagaaca
    17161 agtcaaacta cattcgagaa gatacgcgaa acgtcagttg acgtggtttc gtaaccgttt
    17221 ggatgcacag tggtttgatc ttataagtga gccaaatcaa aaaagtcaga ttgagcaaac
    17281 aatcaaaaaa tggctggagg cggaataaaa tggaagaaaa agtgatcatc gtaggggtag
    17341 aaaccgaagc aaaccagcgt tacttttcag agtcaatgga agagctagtt cagctgacga
    17401 ataccgcatc aggagaagtc gtattcacaa ttacacaaaa gagaccacaa gtggatcggc
    17461 aaacgattat cggtaaaggg aaattacaag aattagttca acaagcggat gcgcatgaag
    17521 cggatttgat catctttaat catgagctaa ctccgagaca gagccaactg attaccgatg
    17581 ctgttggtct tccggtaatt gatcgcgtgc agcttatctt agatattttt gcgatgcgtg
    17641 ctcgttcgaa agaagggaag ctgcaggttg aacttgctca gttagaatac ctgctgcctc
    17701 gtttagtagg gcaagggaaa acattatctc gtcttggcgg tgggatcgga acaagagggc
    17761 caggtgaaac aaagttagaa acagaccgcc gacatatacg gaataaaatt tttgctgtta
    17821 agaaagaatt gaaagaagta gaagctcatc gagagcgcaa tcgccaaaaa agaaaaaata
    17881 gcgagatttt tcagattgga cttattggct acacgaatgc cgggaagtca actatcttga
    17941 atctattgac acaagcggat acttattcga aagatcaatt gtttgcaacg ttagatccat
    18001 tgactaagag atggcgtttt gctgaaggat ttgagattac tgtaacggat accgtcgggt
    18061 tcatccaaga cctgcctacg caactgatcg atgctttcca ttctacatta gaagaaagtc
    18121 aaaatatgga tcttttgcta catgtagtcg atgctagttc tccagatcgt attttacagg
    18181 agcagactgt cttaaaattg atggatgaac tgaatatgaa agaaatgccg atactgactg
    18241 tttacaataa ggcagaccag atcgacccag cgatgttcac gcctacgcta ttcccaaatg
    18301 tattgatttc tactcagacg aaagaaggta agatggcgtt gatcgaagca atcaaacgtc
    18361 agctgatgga actaatgacc ccttatacac tttctgtgcc aagcaacgaa ggacagaaat
    18421 taagcgagtt gagaagacaa acaatggttt tggacgaagc gtacatggaa gaaacaaacg
    18481 aatatgaagt taaagggttt gcaaaaacca attcaaaatg gttaagaaaa aaccaataaa
    18541 attttatttt tttaa
//
LOCUS       DAZAFF010000053        17943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000053 DAZAFF010000000
VERSION     DAZAFF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.053"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..930
                     /locus_tag="ACG9BB_001746"
     CDS             100..930
                     /locus_tag="ACG9BB_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFN2837686.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(1174..1423)
                     /locus_tag="ACG9BB_001747"
                     /pseudo
     CDS             complement(1174..1423)
                     /locus_tag="ACG9BB_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1614..1808)
                     /locus_tag="ACG9BB_001748"
     CDS             complement(1614..1808)
                     /locus_tag="ACG9BB_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837687.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(2313..2498)
                     /locus_tag="ACG9BB_001749"
     CDS             complement(2313..2498)
                     /locus_tag="ACG9BB_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837688.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(3348..3689)
                     /locus_tag="ACG9BB_001750"
     CDS             complement(3348..3689)
                     /locus_tag="ACG9BB_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN2837689.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(3689..3919)
                     /locus_tag="ACG9BB_001751"
     CDS             complement(3689..3919)
                     /locus_tag="ACG9BB_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN2837690.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            4206..4544
                     /locus_tag="ACG9BB_001752"
     CDS             4206..4544
                     /locus_tag="ACG9BB_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837691.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(4749..5311)
                     /locus_tag="ACG9BB_001753"
                     /pseudo
     CDS             complement(4749..5311)
                     /locus_tag="ACG9BB_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(6043..6624)
                     /locus_tag="ACG9BB_001754"
     CDS             complement(6043..6624)
                     /locus_tag="ACG9BB_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2837692.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(6807..7433)
                     /locus_tag="ACG9BB_001755"
     CDS             complement(6807..7433)
                     /locus_tag="ACG9BB_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFN2837693.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(7455..8786)
                     /locus_tag="ACG9BB_001756"
     CDS             complement(7455..8786)
                     /locus_tag="ACG9BB_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFN2837694.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(8941..9342)
                     /locus_tag="ACG9BB_001757"
     CDS             complement(8941..9342)
                     /locus_tag="ACG9BB_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFN2837695.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(9428..9634)
                     /locus_tag="ACG9BB_001758"
     CDS             complement(9428..9634)
                     /locus_tag="ACG9BB_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837696.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(9729..11231)
                     /locus_tag="ACG9BB_001759"
     CDS             complement(9729..11231)
                     /locus_tag="ACG9BB_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFN2837697.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(11249..11704)
                     /gene="argR"
                     /locus_tag="ACG9BB_001760"
     CDS             complement(11249..11704)
                     /gene="argR"
                     /locus_tag="ACG9BB_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN2837698.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(11802..12419)
                     /locus_tag="ACG9BB_001761"
     CDS             complement(11802..12419)
                     /locus_tag="ACG9BB_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFN2837699.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            12640..14013
                     /locus_tag="ACG9BB_001762"
     CDS             12640..14013
                     /locus_tag="ACG9BB_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2837700.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            14088..15200
                     /locus_tag="ACG9BB_001763"
     CDS             14088..15200
                     /locus_tag="ACG9BB_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN2837701.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(15272..16909)
                     /locus_tag="ACG9BB_001764"
     CDS             complement(15272..16909)
                     /locus_tag="ACG9BB_001764"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFN2837702.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(17077..17685)
                     /locus_tag="ACG9BB_001765"
     CDS             complement(17077..17685)
                     /locus_tag="ACG9BB_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN2837703.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         5089 a         3652 c         3131 g         6071 t
ORIGIN      
        1 ataccaatac tttatataat tcgcgagcgc tttctttgaa ttttttaatc tttcgtagca
       61 gaatgagaat gtattaaaaa tagattagga ggaattttga tgtttagaca ccagaaagaa
      121 ttgcagtttg aagcaaaacc agatcgtccg aatccattaa tcgcgaaata tctgcaggag
      181 ctgatcggcg gccaatatgg cgagatgagt gtagctatgc agtacctgtt ccaaggctgg
      241 agctgccgag gagaagaaaa atataaagat atgctgatgg acattgcaac agaagaaatc
      301 ggccacgtgg aaatgttggc gacaatggtt gctcgtttat tagagggtgc gcctgttgaa
      361 gatcaagaag cagcgatgca aaaagatcca acaatcggtg ccattatctc tggaatgaat
      421 ccacagcacg cgatcgtaga tggcttagga cctcgtcctg ctgacagcgt gggcaatcca
      481 tggagcggtg cctatatcat tgcgagtggc aacttgttag cagactttag agctaattta
      541 aatgccgaat cacaaggaag acttcaagta gcacgtattt acgaaatgat cgatgatcgt
      601 ggcgtaaaag atatgctgtc tgttctcctt gcaagagata gctatcatca aaatctatgg
      661 gaatgtgcag tgaaagaatt agaagaaaaa gaaggttcaa tcttagtccc aagtactttc
      721 ccgcgtgaca aagaacgaaa agacattgct tatgacttct ataacttctc agagggagac
      781 caaagtagtg aaggttcttg ggctagtggc aaagcactgg atggcgaagg cgaatatcaa
      841 tggttgaaag aacctaagat cgaaggcgct gcaccaaaat tagaaccagt aacaccaaaa
      901 atgtatggga cacctccatt caagaaataa atatctaccc gtagaaacac ccgttttttg
      961 ttccacacgc tttgtgagca aaactgacta aagtcattaa tcataaccac ccttcaaaag
     1021 ggtggttttc tctagagcta taagctcaaa tcaccggcca gcgcctaaag acgctggctt
     1081 tcatatttta tgttcaagcc atattgcctt tgccagtttt gcccacctct taaaaggttt
     1141 atgtactact ccttagccct taaaaggatc gtcttactct cgtacactca gcttatctaa
     1201 cgcactatca tgtttttctt gttcttgaat gtactttttg atggtcgctt cattttgtcc
     1261 tacttttgtc acatagtatc cttccaccca aaagtgtcga tacccaaatt tatattttag
     1321 atttgcacaa tctcttgatg atctcctgaa tattggctcg atattgatta taaattattt
     1381 ttcttctata ctttggtgtg aatacaatgt ggtatttaca catctacttt gtacgggcta
     1441 aactatttcc ctttattgct ccacccgcat agcaagtggt tttgtatttc aatcgctcgg
     1501 cgcgctcaat taggtctaaa gacaataaat ctaaaaaccc gctgcttcac tgacagaaac
     1561 aacgggtttt cagaaaaaaa tgaaatgaaa aagatctctt acggcaatct attttatttt
     1621 tttgattttt tataaatgga aaatgcttca agaagtccaa gaaataaagc cgtataaaaa
     1681 caagaaccta tgaaatcttt atttattaga tattcaacag aaatcccgat aacactagcc
     1741 aacaatatat ttatcagaag tgtaaaataa ggatgctttt tatagaatag aactagtgtt
     1801 gttttcatag actctccttt cttcatattt ccaattaatc acaaattcac ggcattacta
     1861 gaaaaattaa aggggcgcag aagtggaaaa atactcttcg actttcccta caaaccaggc
     1921 catcttgtgt ttaataacta taccaaatct aaggtacctc taaatctaaa ttccatttgt
     1981 ggcggtgacc tccaattttt aactcttcta tctcccatcc agtatacata accttgatac
     2041 ttcgaccact tatatatcga acacacaaat acctatccat tgcaggagta ctacctaact
     2101 tagttgctac ttgcatctaa ataacagctg aaaagatagg acttactctt agtaaagtat
     2161 ctgaactatt aatttttaaa ctatcatttt tatcctcaac tacatgcaca tctgttgcaa
     2221 cttcactttc tgtctcatcc agataaatga ttaaggaatc gggaattgca atatcttcag
     2281 ctgacgctgg aatagttgag ctaaattagt catcatatct ttattataaa ataaccaaca
     2341 gcaaaaaata caatgaaaaa caaacttgga actaatgtat cattaataaa tgttgaaaaa
     2401 ttcagcaact cattactgta tgattttagc agcccaacca acatataccc tataaaagct
     2461 aataagatgg ttaggaatcc tttagaattt ttactcattc atagacactt ccttaaataa
     2521 cttttattat agaaggtatt gtaaataata atattgacaa aaagtgactc aaaataaaaa
     2581 aaaccttaca ttatactgga atgaactgta ttgatagtgt acctaaaagc tctactgaca
     2641 taagatcgtg taatcggtaa agtaatttta ttaattatac gggatgtaat caattagaga
     2701 ataaactaat cataaaaggt acttttacat tcataattgt cgctttgaga ttctactttg
     2761 gttcactatc tgacccaatt gcacattttc gatcattaca agtattggtg tcgttagaac
     2821 aactaaataa aaactagcgg tagatttttc agaatctatg ctaatttctg tatggatgtt
     2881 ccagtgatgg caaatttctc ttttcattgt ttttcttgtc gcatgatatg cctttttgtg
     2941 ttattcagtt aaactataga aattctttcc tagacagatc ttgatcccaa attttacgtt
     3001 tttgagcatc gatatagttt aaaatgacat tactcatatc taaagagttt agatttttta
     3061 ggactaaaac tgtcagttgt tctttttctt ctttctgtta aagtattgtc gcgacttttc
     3121 gtatacgtta agatatgtcg tgaaaaaaca agttttgtga cacaaaaaaa cgaatgactt
     3181 gaggaaatca cctcaatcat tcgtttaaaa tgctatatat tctttagttt aagatacgga
     3241 aattaatgcc atcgaccaca ctagtagaat gaacgacatt tacttggcct ttttttaatt
     3301 tcgttatgtt tagcatataa aaatattata gagtttactt acaatgctta aaacatatac
     3361 tgtataatct gatcaatttt agccatgtct tgcaatggta agttttctat ttttttgagt
     3421 tttcgttcct taaaatcaag agattttaat tgtgtaataa ttacttgtcc ttttatatct
     3481 agctcttcag gtaatgagta acgtgtcggt aaattcttta tagtggaagt aattggacat
     3541 atgaccgaaa acattgtttt acggttaaat tcataacgag agacgactag tcctggtcgt
     3601 cttttttgaa tttcttttcc tacggaggga tcaaaatcaa tccatacaat atcacctttt
     3661 ttaggaatat aaattctttc ttctgacact ataaaatttc tctcccttca ggagttaaat
     3721 cttcccattc gtctttctca taatactcgg cctcttcccc accactaaat ggatcttcaa
     3781 tttttggaac taacgtaatt gttccatcat cagaatacac aacgatatat tcttggtttt
     3841 ctgaaggttt ctttccgtta tcagaaggta aagttaccac tacagaactt ccttgtaatc
     3901 tagattttgt aacaagcatt atcatcactc ccttaataaa agtatactat ataacactaa
     3961 gtaacgcaaa aatccccttt gtaaactatc tagaaaattg ttttgtttaa agtatatctt
     4021 attggatata aatagaaata aattgtagcc agttcatttt ttctttaact aaaaaaataa
     4081 tccaagaaac attgaaccta tggatccaaa agctacccaa atactatagc taaccccccg
     4141 ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat ttcaaagaca acaggccatc
     4201 ctatcatgcc cgattataaa caaaaagctg gctattcctt ttgctctgac aaatttcaat
     4261 tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac tgttcggatc aaagaatgcc
     4321 cgcttcaaat agaaacattg gttcaacgaa tttcaactag acaagacttt gctattgtag
     4381 aacgtgagat cgtagctatt tttgtagaag aagggatttt acatgatcca actcatatca
     4441 atgtggataa atggcaacct cttatctata aattcagaga atatacaact gccaaccagt
     4501 cgttaacttt gaattttaga ttccaagaat tcaaagagtc ataatgataa acatcgttag
     4561 gtataaataa aaaagactcg cagcacgagt aaaaaacaaa tagaagctat acattagaaa
     4621 ggtcgactgt gaccctatag atgcagtata tgcagcttat gagcatttct tctgttctct
     4681 ttgtttttgg gatctgtgct gaagtctttt tcgttttttg ccgctctttc tttgaaaaat
     4741 cgattatgtt ataccttctc cattactaat tcaggcatct gctgagtaat gatttttcgc
     4801 gcaaaagaaa tatctgttac ccaaggttcg atttgttctt cagtcaagac aagcggcatt
     4861 cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag tcattatgat tgattctgcc
     4921 attttttcat cctgatggct acgataaaaa ccgccaatat acactatttt ttccttattt
     4981 gaaaacataa atttgtcttt ttcttcattc cactcattgg gaaaacacaa cgattttgca
     5041 aaaatggttg atgaaaggtc tttttttctt ctactgtctc tgcacgggca ttaatcatca
     5101 attgtccttt tttaaatccg gtaaatcccc acttcatgat ttccggagtg aggatgtttt
     5161 cttcctcttt tcttaacgtc acaacaagat ttgacggaaa aacttctcca gctgctaatt
     5221 ctgctgcctg cttcggttgt ttcgtctggg cttctcgata ataattctga aggagctctg
     5281 actctagatc aaaaaaataa cgaccacaca tcctaatccc tcccaatctt tttttctatc
     5341 atacactgca acaagaagtt cgcaccaaca aaacgctttc aatttttgta gaactaaaaa
     5401 agttgtgaca agtgacaaac tctccagtga atggtattct cctgccaact cctcggtcac
     5461 aagtcacaat attcaacaaa catgagaagc tgctttttga aattgttcct tgtgactcgg
     5521 agtgaaacgg caggcttacg acctctgggt aaacggtgtt caaaagccag tttctcggta
     5581 ttaagccaaa tttatgcaaa aatgtagaaa gctattttca caaaattttt cttaatactc
     5641 gaaactaagc gactttttca caacctcttc cttaaacggt gttcaaaagc ttgcatctgc
     5701 ggtaataagc ccaaccaaac gaaaaatatg agcagctatt tttatttggc tgttcttatt
     5761 acttgatgca gggcagcttt ttcacaacct ctccactaaa cggtgttcaa tcagctaacc
     5821 ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttgccgtt tgtttctcta
     5881 atactcaggt caaagcgctg atttcacaac ctctaataca cggtgttcca gaagtagctt
     5941 cttcgaaaaa taagccgaaa tttcacaaaa atttgagaag caattttcgt aaattccttc
     6001 ttatttcttg aagctgacca cttctgtcac aacctctaat tattaaagaa ccttttgcga
     6061 agttaacaat ctttctttga tcaattgaaa attctgccta agtagtgata gatcctgtgt
     6121 gactttcaac aataagatac ttccctggat ttcagcaatc aacgctttgg cttgacggtc
     6181 tgcttcttca tcggacagtt tttcttcaga aagtgccagg atattttgtt tcgtatactt
     6241 gacccattga ttgactaatt ctagtagcaa cagacggtat tcttcatcca ctgtactcaa
     6301 ctcaataatc aaattaccta caggacagcc atgataagtg atctgtgtct catgatcagc
     6361 gaaaatccag tccaacattt cttctaatgc ttcttctggc tgttttccag catttaagat
     6421 gtcttcgaac aattctgtct gccatacgtc catatgcgca tggatcaccg ctagacaaac
     6481 ttcttttttt gaagaaaagt aataataaaa ttgacctttt cctgtatcag aagctgctag
     6541 aatatctcgg attgacgttt catgatagcc ttttttataa ataagatctt tggtcgcttc
     6601 caagatcttt tcctttgttt tcaatgatat tctccttttt catttctcat agtcctatat
     6661 tatacagtaa attcatcatt ttttcttctt aatcgtttaa aaaagactgt gccaaattat
     6721 ctgacacagc cctcactata aaactgttct catatagcta gatgttcatt gattcaagaa
     6781 ctgcgaggac aaaagcaatt cctatcttat gatagttgct ctaatacttc ctctgttgtc
     6841 atcacttgat ctgcaataaa gccaaagttc gtcaacgctg catccattgc ttcttggcca
     6901 ggagcaccaa ttgcatctgt cgctaccaca acattgaatc cactctctac caattcacgc
     6961 atatgggaat cgctacacaa gtttccgttc attccaccaa gaattactgt gtcgattcca
     7021 ttttttctca attggagcgc caaatcattg ctttctggtc caaagatttt atgaggagag
     7081 ctgatgatcg tattttcatc caaataaggt ttcaattctt cgataaaatc ggctcctgaa
     7141 ccttcttcca ctgattcata ttgacttttt ctagcaaaca tctgctgctc aagcatcata
     7201 tgttctcctg caccgccaaa ttgccattta tggtcatgtg ggtaaaaata atgaggagaa
     7261 ataaagagtt tgtatccttt ttcttttgct tgttttacca tttcaatcat attttcgatc
     7321 gtattgtatt tttctaagat atcttttgtc aaaccaaagc caccgcctgc tggtttcaag
     7381 aactcgactt gcgggtcagt gagaactact gctgtatttt tattattgaa catggtggat
     7441 tctccattcg ttatttattt taatacatca tttagagctt cactcattgt cggatgagaa
     7501 tagatttgat ctctcaataa cgtataagga agttttccat tcatagctag cgaaatcaag
     7561 ttgatgactt catgagaatc ttccgcatag atacttgccc ctagaatcaa gtcagtttca
     7621 gggtcgacta aaactttgaa tacaccttta ggatcttcta atacttgtgc tttagggaca
     7681 ccgcttgtca tatacttgaa taatttgtaa ttgacgcctt gtgctttggc ttgtttttct
     7741 gttaaaccta cattggataa agtcggagtg ataaatacac tgtaaggcac agctttccgg
     7801 ttattcgttg ttcgtttatt ctcgcccttc aactgatcca taatgattcg ataatcatct
     7861 agagagatat aagtgaactg caatccgcct ttgacgtctc caatcgccca aatgttgtct
     7921 gcatctgtac gaaggtattc atccacagcg attgctccgc gatctgttag tttaacatct
     7981 gtattttcta atccaagtga tgctgtactt ggttttcttc cagttgcagc caaaactttt
     8041 gacgcttgga ttgttacttc ttggccatct tctgtaaagg tgacggcagc tgttgattct
     8101 tggtctgcta cttgatctac agatacacct agatggaatt cgattcccgc atttgtcatg
     8161 tcttctaaaa tcatttgcgc gatatcttca tcttcacgtg gaagaaattc agaatgggca
     8221 tcaagaacga caactttaga tccgtattca ttgaacattg atgcaaattc caagccaata
     8281 tatccagccc cgatgatcac taatttttcc ggtaattgtt cctgatccat cgcttgcgta
     8341 gaatcaatca agtagcgact tgtattcaat cctttgattg gtaaaatgat tggttcagaa
     8401 ccagtattga tgaaaatccg ttcgccttca acaatcactt tttcaccatt attcgtcact
     8461 tcgatttgat ggttagaaag aaaacgtgct gtaccgtcca tcactgtccc agttgcctca
     8521 tcgctgatca tatgataatt tttggcgcga agcatgcccg tcaatttttc tttccgagca
     8581 acagcctctg taaagtcgat tcctctttct ccattgaaaa tcaatgattt agaaggaata
     8641 cagccgatgt tgatacatgt tccgccatac atacgtgttg acttttcaac gactaaaacc
     8701 gattcacctt ttgaagctaa cgccttcgct aaagtcttac cgccctttcc aaaaccgatc
     8761 acaatatttt gatatttttc catcattttt ccctcgcttc ttttttacct accgattggt
     8821 acaaaaatat gataaccgat aattgtctat tctccaaatc atctgcttta tttgtcaaaa
     8881 aaaaccagac aaaaagcggt cctttcacgt ttttagtctg gttcgatttc ctttattctt
     8941 ctaaacaatc gttacttcta catcgatatt tcctctagtt gctttggagt aagggcagaa
     9001 ttcatgcgca tcttctgcaa ttttttgggc ttcttcttcg ctgatgccat cgatatgcac
     9061 ttctaatact acaccgatct gatagccgcc atcatctaaa ttataaagag agacacgtgc
     9121 ttttactgtg gactctgctg tcattcctgc ttctttgatc atatgttgta ccgccccatt
     9181 gaaacaagaa gcataacctg ctgcaaataa ttgttctgga tttgttcctt ctgcatggat
     9241 tcctggagga gtgattttca tatgcatcga gccatttggt gcctcgactt ctccattgcg
     9301 cccgccatgg ttgatcattg ctgtttcgta tgcttttttc attgtactgc tcctttcgat
     9361 tgaccatttg ctacttggct ataattatag tgtatgacaa gtaaggcgct ttctacaaaa
     9421 attctgctta ttcgaatacc agaatgccgt cttctactat ttttccaaaa aacaaaaaaa
     9481 tactactcgt gaacaacagc gtcatatttg ttccaaaata aatagcaatc ttttctacat
     9541 aatcttttat atgattgatt ttaagataca tatataccag aaagttgacc ccataaacca
     9601 atactgttaa aaaaataacc atattcgtta ccattttttc ctccttttgc tggtctccct
     9661 accagcactc gatttaacca taccactttt tttgccgtcc aagcaactag cctttcaaaa
     9721 agactggttt attctgctgt taatgattca agatcagaaa aatgtccgaa taattttgct
     9781 aattttacta acgccaactt gttctttccg aattcatcat tttctatctg taatactagt
     9841 aacggctcat gtaagcttaa acggatcaaa aaccagccgt ttccgtatgg ctcttctaga
     9901 tcatatcgaa taccttcttc attttccggg tcgaagttca tccctttttc ttcttccaac
     9961 agtcgcttca tttcttctaa tatcttgttt ccttgttcgc gtggttcatc gctgaacaat
    10021 tttaatcgga tttcttgtac ttctacgggc tgtttcaact ctttgatttg ttcatctaat
    10081 gtgatacctt ttctttttaa ttctggcaaa agcatcagga tctttgcgat gacatatgca
    10141 ccatcatcca gaaaatagtt ttccttgaat gcagcgtgtc cgcttgtttc gatggctagc
    10201 tgtgtatctt tcccaagacg gttaagttct aatgctttat tgattacatt tcgatagccg
    10261 gaaatataac gaacttggat gcctcctttt tcttcaatga atctttgtag atgagaagaa
    10321 gtaggggaat tcgtcacgat tcttgttcct ggatgctctt ttaatacgat tgtacttaat
    10381 actgcgatca aattatttcg attgatcact gttccagaag cactgacaac tgcagaacga
    10441 tccacatctg tatcgaaaat gatcccaaga tctgcttcat ttgctaaaac agcctgcttg
    10501 atactttcca tcgcttcttt attatcagga tttgggatat gattaggaaa tcggccatct
    10561 ggttccaaaa attgggaacc tgttgaatcc gcacctaagc tattcaatac tttttcagca
    10621 aagaatccac cagcaccatt tccggcatca acaatgattt tccaaccatt tagtggtttg
    10681 ctttctagtg ttccaatccc ttttttaatt tttgtgacta aatcttgcgc ataaggtgtc
    10741 aaaaggtctg ctttttctac gcttccagtt tgttcgcttt ttattttttg ctgctgtgat
    10801 aggataaaag caatatcttc tttttctgct cctccttctg aggtaaagaa ttttattcca
    10861 ttataataat agggaagatg gctagcagtc agcataaccg cagcatcgca cgagaactgt
    10921 tcaaattgag tggacataaa catagcaggc gtagtagcta attcgaaatc gatgacatgt
    10981 atcccttgtt ctttcagact ttggatcaac gcctctttca gtgctggtcc gcttatccta
    11041 ctgtcatgac caatcccaat cgtccatttt ttatttggat cggttgattt cttctttttc
    11101 tttatccagt ttgctagtcc ccagccgatc ttttttacca tgtctgtcgt caagtttact
    11161 tcatatcctt caactggtaa tgcgattcct cgaatatccg atccattttg cagcgctttc
    11221 atatctttca tataaaagtc ctcctcattt attccctcat tttttccagc ttttctgcaa
    11281 atcgctgtgc ttcttcttct gagcggcaaa taataatgaa tgtatctaca ccggccatcg
    11341 ttccgacaac ttcagggtaa gccacttcat ctagccaatt cgttaccaca tcggcaccat
    11401 ttcgttcagt caatacgata accatgaact gaacagactg gatacgcaaa acctcgcgtc
    11461 taattgctgt acgcaaacgt tcttcattta aagaaacaga tggctggtta aataatgtat
    11521 agcgtactat tttgttttca ttttgacttt ttactaactt catttctcga atgtctcgag
    11581 aaatcgttgc ttgagtcaca tcggcgcctg cttcatgcag ccgcgcaagc agctcttcct
    11641 gtgtcccaat aggttgttct gtaatgatct ggcggatcag tgcttgtcgc tgtttctttt
    11701 tcatattaga cacctctcaa tgttgattat agcaaaagat aagtaatatt tcttcatatt
    11761 ttggtatttt ttacttttat tttttgtttt cattcaggtt gctaaccttt ttctatttta
    11821 acacgcaaaa tcgtcttcat tttttcaggt gctgattttc ttggatcgct gatgtaaatc
    11881 tctttgtgtt ctttagaagc tctttcatag ccttcttttg cgatgaatgc ttctaatttc
    11941 tcaaacgttg ctggttcttc ggcatagggc ccgacgtgta agatttgggc acaaagacct
    12001 tcttccatct ttttgaatac tacttgttct ttcaagtctt ccggaagttt gctagaacgc
    12061 tgaatggctt cttgtgctat cgcttctgta acaaagtcag gctgtcggat catcaattga
    12121 taagaaaaat gttctttttt catcactggt tcatgcaaat acttttcttg cagtccccat
    12181 tgtccttcca gtggatagac agtatacatc tgataatcag gaatcagcca atttttttta
    12241 ggcgacatgc gtattgcata gctgactgca tatagacagg ctactcttcg tttaaagtct
    12301 tcctgattcg ggtcgccgat ccctctgatg ctaaagaatg attttttagg aacctctagt
    12361 atttgcgggc tttttttcat actatagctc ttgtctttac gccattcaat tttttccatt
    12421 tttcttcctc cgcgaaattc catttagtta tttattatca tacctctttt tgagtgactt
    12481 ggctaatcgt gttgacacta tttttcagtt tgtgctagaa taagtgaagt aaataaatcg
    12541 tacagataac acgtttcggc gtgtcgagag agaggcggtt tctattttta taatagaaat
    12601 cactctcttt ttttacgaaa ttttttagag gtgatgtaaa tgacaaaaca aaaacagctg
    12661 cttttagtac ctgcagtaat tgcaacaggg atgatgtctt ttgcgggcgt attgatcgag
    12721 acggccatga atgtaacttt cccaactctt atcagacagt ttgatttaac gactgcacaa
    12781 gtccaatggg taacaacgat ttatcttttg atgatttcga tcattgttcc gctgtctact
    12841 tatttgaatc gcaattttag tctcaaaaaa ctctttttag cttcaaatct gcttttcttg
    12901 gcaggtataa tgatcgattt cttctctcct accttcatgg tcctattgtt tggtcgattg
    12961 ctccaaggtg cagcgacagg tatcgctctt ccgttgatgt tccatattat cttgacattt
    13021 gcccccctca atcgacgagg cgcgatgatg ggtgtcggta ctttgacaac ggccattgct
    13081 ccagcaattg gccccactta tggaggcatt atgacttcac attttacatg gaatcatatc
    13141 ttcctgtttc ttgtgcccgt tttgtttctt tctttgatta tcggctggtt tgctattccc
    13201 gagatggcag tacaaaaaag tggttatttg gattggataa gcgttttagg aatttgtctt
    13261 ttgtttagtg gaagtctaac atttttgagt acgttgggaa cagtttccag ctggattgct
    13321 ttaatcatcg gcggtattgg ctggttcctt ttttactatc gaagtaaaaa agtggagcat
    13381 ccactgatcc gactgtctat tctttcaaat aaaacattcc gcttgttttt gattggcttc
    13441 ttagtttatc agtttttatt acttggtgta tcttttgtct tgccgaattt ccttcagatc
    13501 gttcaaggaa gtagtccttt tgtcgctgga ttagcgatgc ttcccggcgc agcggttggt
    13561 gctttgcttt ctcctttttc cgggaaaatg ctggatcaat atggtccgaa gaagccaatt
    13621 tttgctgggc tgattctttc tttgattggt tggctagcat tattcctact cataggacac
    13681 gcttcagtgg gtgtattgat tgcttgtcat gtgttttata tgatcggtat cggattatct
    13741 tacagcaata tgatgaccac cggaatgaat gcactgaatg aagaactaca aggagacggg
    13801 aacgcaatgt tcaatacctt gcagcagttt tctggcgctg tggctacttc attagtagcg
    13861 gtcattatca attacgtcca gcatcatact tcccataact atgaagtgtc tactacatta
    13921 ggatcaaaag cagcattagg cgtcttgttg ctgttgcttc tcgtaagctt tgctcgattc
    13981 gcctattatc tgttctttgc taaaaaagcg tagattccat atacggcagg tgttgcatct
    14041 gccgtttttt tgtgtgaaaa tggttgtttg taaaaggagg cttttttatg gaaaaaattg
    14101 ccattatcgg tggcgggatc attggtatga ccctagccag ttacttagat acatcaaaat
    14161 tcaatgttgc tttatttgat gaagggacag gacaggcaac taaagctagc gctgggatta
    14221 tctccccttg gttgtcgaaa cgcagaaaca aacaatggta tcgtctggct aaagacggcg
    14281 ctgctttttt ccctaaactt ataaaagatt tccaacttga tcaaacgatt tacgcacaat
    14341 cagggacact tttgcttcga cctgaaaaag atttagctga attggctgtt cttgccgaga
    14401 aacgaaaaga agaagcgcca gaaattggaa acatcgaatg gctttcacct gaacagacat
    14461 ctcaagcact tccgttattg aaaccagcag tttcactgcg tatcagtggt ggtggtcgtc
    14521 tggatggaaa agcatattta gaacatcttg gtcagcgttt agcacaaaaa caaatggagc
    14581 agatccaggc aaaagtcact ttgcataaga agagcgattc ttggctcatc aagggaccgt
    14641 ttggagaata cacagctgat cgtgtaattt tgacacctgg cccggcactt aagcctttat
    14701 tacaagaatt aggctatgct gcagatatac gtccgcaaaa agggcagctc ttagcatttc
    14761 agacatctta taagatgagt caagactggc ctgttgctat gcttgacgga gaagctgatt
    14821 tcattccttt catggatgga aagatccttt taggtgctac ccatgaaaac gatcaaggct
    14881 gggatttggc tcctactagc gaagcttata agggattgac taagagtaca gaacagttct
    14941 tgactgatcc agatcagata aaaaactggc catacgagtt ccgtgtaggt acccgtgctt
    15001 atacgtcaga tttttcccca tttttcggcg tactgccaga tgatccaagc ttactggctg
    15061 ctagcggtct aggctcttct ggcttgacca caggtcctta tatcggctat ctattagcca
    15121 acttcttaaa tactggtcat tggcctacgg aagattatca aaaggacatc tccacctata
    15181 ttacgaaaaa tccatcatag aaaaacagct ttttcaagta aggatcccga gcgatattgc
    15241 gcatggatta cttcttgaaa aagctgttat tttagttcaa tttctgaatc aaattctcca
    15301 aactttcttt tgcttcatct ggcaagagat cttttcctat ctgagcttca atgaatcgta
    15361 aacgaatatc aagtcgttgt ttcaccagat ttttatagga cgtatcctca aaagaaacag
    15421 gaaaatctgg tataggtaaa tagtggatgt cttcaaatgg accaaacgac ttgaattctg
    15481 ccgtttgttg cacgattctt tcaacgattg acaatgtcgt ttcaggtttg atttttttat
    15541 catcgaaata tccagtattc aggacatagc aggctgtttg ctgtttcaaa aacaactctc
    15601 gaaaagcttg atagtcttcc ctaagaggat agacacggaa aggattagca aatggttcaa
    15661 tgactagctc atctagctgt ctttttgtag tgatattttc cgcgtctgat cgttttgttg
    15721 ctaatgttgc accaaaagac gctgctaaaa ctgggtcatt gatcttgata attggcggaa
    15781 gcgaatcatc tttcatgatc caaaaaacag catcgatctt ttcgctaaga tgatcgactc
    15841 ggtcgggtgt cgcataacgt gatttgactg tccgcccatt tccgtttcgt atatctcctg
    15901 ttactaggac tttttctcct ttacttgtac atgtcacgcc cacattctga caagtaagga
    15961 aagaagaaat ggccggatca tcagacggat agtcttgtgt tttatcaaag taagaaggtt
    16021 ccaaggcagt agcaaaacca ttttttcgag aaataacgaa agcatcgtca tgcaagatcg
    16081 aaacagagtg accttccgta tgttttgcta aggtaatcgt tgattttcca gaaccggaca
    16141 acccaaatac agccatcgta taggtctggt cattactagc atattgtttc atcccaccgt
    16201 ggcaggcaat aaagccattg cgatttccca atgcccaagc aagcgtcaaa gtcgcttttt
    16261 ttaattctcc aaagtaacgt aatcctagca cagccgctgt attatggaga gcatcaaaca
    16321 aaatgagtcc atttggaaat gtttcatgtg tccattcggg atcacaaaag atataaagat
    16381 ctccctcatc aaaggaaaca gacttttgat aagtctctaa gatcttcgaa ttcgctggct
    16441 gaaaattcag caaataagaa tacaaattgt tctcaaatcc ttctggaatc agcaaatgac
    16501 tttgcacaga aaattcttca gataggccaa cgattacttt tgtatggtaa aactggcgct
    16561 tgcgactttg atacacagct tctcttaaaa ttttctcgta tttgctttgt tccttgtcta
    16621 agtcctcaaa gatccgccga gcacttgctg ttcttcccac aatgcttcca tcattgaata
    16681 ctagtgtcct aacatcagct ggtaaatcta gttcttcagc atgtgagatt gttaaatctg
    16741 tgatgatcgt tcctggactc ccttttgcta gttgataagc tgtcttccta tcaaaaactt
    16801 catgaacatt attaccgtaa aatgtcgttt ctacaatcga tcgaatactg gataacagtg
    16861 gatttccact tgtgatttcg tttaattcga gactctcttt tgtactcatg gctcctcctc
    16921 attccctaaa caaaaaaacg ttttctttat tatacattat tcgagttttt atttgtactg
    16981 ctgcgctgtg ggaagcaaac gagttatttt tcacacaaaa aaaccgagaa cttgaataaa
    17041 caagtttctc ggtttcatca ttttcctttt atcggcttag taccagccgt ttgctaacca
    17101 gaatgtttta gcagcatccc atgaaccata acgagatgtt acatattgtt ctgctacacg
    17161 ttcttgattg gcagctgaat agtcgccgtt taagtatgaa gcatctaatt gataacggcc
    17221 gatgtaacgt ccgttagtag ctgtgtatga accgcctgat tctttttgtg cgatccattc
    17281 ttttgcagag cttgaagttg gcgctgttgc agcagctggg gcagcaggag ctacttcaac
    17341 tgtttcaaca ggtgcaacgt atgtatgttc ttgcgttgct gtttcagttg tagatgctgt
    17401 attttctgtt gttttgtttt ctgttgttgc tgaagaatca tttgaagttg ggattgttaa
    17461 ttgttcacca acataaatgc ggttgatgtt tgcgatcgaa tttttttcag caattgcatc
    17521 gatcattgag ttgtctcctg caaatttttg tgagatctta gataatgaat cacctgattt
    17581 tactgtataa acttcatcag cgtgagcatt tgtagttcct acaaacattg cagtagcacc
    17641 agcagcaaaa gttgtaccta aaacaatttt cttaaatgaa ttcatattaa actccttttt
    17701 ttctccgttt tttattcttt cgacgctatt aactttatca tttagaaata tttcataggt
    17761 aaaagaaaca tgacatttaa tgacaaaata acgaattatt taatatttgt aacatttaaa
    17821 caaatatttt tttgatacaa aaccataaaa gccttgttgt ataagcaaag taatcgtttt
    17881 tctatgtttt tttatttttg aatgaaaaac gcagattctg taacattcac tgcaataatc
    17941 cat
//
LOCUS       DAZAFF010000054        17723 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000054 DAZAFF010000000
VERSION     DAZAFF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.054"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..834
                     /locus_tag="ACG9BB_001766"
     CDS             22..834
                     /locus_tag="ACG9BB_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFN2837704.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            831..1631
                     /locus_tag="ACG9BB_001767"
     CDS             831..1631
                     /locus_tag="ACG9BB_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFN2837705.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1792..3060
                     /gene="rseP"
                     /locus_tag="ACG9BB_001768"
     CDS             1792..3060
                     /gene="rseP"
                     /locus_tag="ACG9BB_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFN2837706.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3128..4837
                     /locus_tag="ACG9BB_001769"
     CDS             3128..4837
                     /locus_tag="ACG9BB_001769"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFN2837707.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5049..9401
                     /locus_tag="ACG9BB_001770"
     CDS             5049..9401
                     /locus_tag="ACG9BB_001770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFN2837708.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9545..10018
                     /gene="rimP"
                     /locus_tag="ACG9BB_001771"
     CDS             9545..10018
                     /gene="rimP"
                     /locus_tag="ACG9BB_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFN2837709.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10042..11217
                     /gene="nusA"
                     /locus_tag="ACG9BB_001772"
     CDS             10042..11217
                     /gene="nusA"
                     /locus_tag="ACG9BB_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFN2837710.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11239..11532
                     /locus_tag="ACG9BB_001773"
     CDS             11239..11532
                     /locus_tag="ACG9BB_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFN2837711.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11529..11840
                     /locus_tag="ACG9BB_001774"
     CDS             11529..11840
                     /locus_tag="ACG9BB_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFN2837712.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11853..14159
                     /gene="infB"
                     /locus_tag="ACG9BB_001775"
     CDS             11853..14159
                     /gene="infB"
                     /locus_tag="ACG9BB_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFN2837713.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKNHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14186..14533
                     /gene="rbfA"
                     /locus_tag="ACG9BB_001776"
     CDS             14186..14533
                     /gene="rbfA"
                     /locus_tag="ACG9BB_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFN2837714.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14641..15145)
                     /locus_tag="ACG9BB_001777"
                     /pseudo
     CDS             complement(14641..15145)
                     /locus_tag="ACG9BB_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15310..17673
                     /locus_tag="ACG9BB_001778"
     CDS             15310..17673
                     /locus_tag="ACG9BB_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFN2837715.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6042 a         3146 c         3906 g         4629 t
ORIGIN      
        1 ttaagtatag gaggaaaaag aatgttacgt ttttttccgc aaaaaaacaa atatattcaa
       61 gaaaaaagcg aatttcaatt taataccgat ggaccaattc ctcagcacat tgccatcatc
      121 atggacggca atggtcgttg ggcgcaaaac cgtcgtttgc cacgagttgc tggccataaa
      181 gaaggcatgg aaacagtaaa gaaagtcaca aaaaaagcat caaggcttgg agtgaaagtg
      241 ttgacgcttt atgccttttc gactgaaaac tggaagcgac caaaagatga agtaagtttt
      301 ctcatgcaac tacctgtcga cttctttgat acgttcgtac cagaattgat taaagaaaat
      361 gtcaaagttc atgtcatggg ttatgaaaac gtattgccag aacatacgca agatgctgta
      421 agacgtgcaa ttgagcaaac aaaaaacaat actggaatgg tattgaattt tgctttgaat
      481 tatggaagta gagcagagat cgtgactgct gtccaagaaa ttgccgaaga agtagcaaaa
      541 ggagaaatac acgcagaaga aatcgatgat gaattgattg ctaagcattt aatgacaggt
      601 ttcttgccta aagagttaca agatcctgag ctgatgatcc gtacaagtgg agaagagcga
      661 attagtaatt ttctgttatg gcagatcgca tatagtgagc tgtattttac aaaagcgctt
      721 tggcctgatt ttgatggtgc gcatcttgaa gaagcaatcg cctcttatca aaatcgagac
      781 cgtcgattcg gcggtgtaaa aaaaacagaa gatgagaaag gggatcagtc atgaaacaac
      841 gtgtgatcac agcagctgtg gcgctagtct tgtttattcc gattatatgg atcggaggca
      901 tagcggttga gcttacagca gcaatcttag cggtagtagg agtttacgag ctgttccgga
      961 tgaaaggatt gacactctta agttttgaag gagttttatc cgccgttggt gcagtgttac
     1021 ttgttttgcc aaaagaaaga tggtttttct ttcttccaga gaatgtaagt acatttatgt
     1081 tattttatct tatagttatg attcttttag gaacatcagt tatttctaaa aatacgtata
     1141 cgattgatga agcaggtttt cctgtgcttg tcagcttgta tgtgggaata ggtttccaga
     1201 attttgtcaa tgcacgaaca gaaagcttgg ctgttttgat atttggtcta tttattgttt
     1261 gggcaactga tatcggagca tatatggttg gtcgacgtta tggtcagcat aagttaagtc
     1321 ctgatatttc gcccaacaaa acgattgaag gtgcgctcgg tggtatcggt agtgctgttg
     1381 tagtcgcatt tctttattta ttgctatacc cagcaaaaga actattcaat catggtatgt
     1441 ttgtcatgct tctgatgacg attatttttt cagcagtcgg acaatttggt gatttggtcg
     1501 aatctgcaat caaacgtcat tatgatgtga aagactcggg aaatatctta ccaggccatg
     1561 gtggaatctt agaccgtttc gacagtttgc tgtttgtatt tcctatcatg catttatttg
     1621 gcattattta gtaagtatac ctgcgtttct ttgtgaattt cataaaagac gcaggttttt
     1681 atttttcatt atattgatac tattggtgtc tttggtatac tatggtaaaa ttatagtgtt
     1741 ttatcgttgt tttataaaaa acacaaaaaa caagtaaagg agaaaaaagc tatgaaaacg
     1801 attctgacat ttatcatcgt ttttggtata ttagtgattg ttcatgagtt tggtcatttc
     1861 ttctttgcga aacgatcagg aatcctcgtc cgcgaatttg ctatcggtat gggtccgaaa
     1921 atttatgggc atcaagcaaa agacgggaca acatatacgc ttcgcctatt acctattggc
     1981 ggttatgttc gaatggcagg caatggggat gatgaaacag aaatggcacc aggaatgcct
     2041 ctttctcttt tactaaattc tgacggcatc gtagagaaga taaatttaag taagaaaatc
     2101 caattgacaa atgctatccc gatggaattg agccgttatg atttagaaga tgagctgact
     2161 atcactggtt atgtgaatgg agacgaaaca gaagtagtaa cctaccctgt tgatcacgat
     2221 gcgacgatta ttgaaaacga cggaacagaa atcagaattg cgccaaaaga cgtacagttc
     2281 caatcggcta aattgtggca gcgtatgctg accaactttg ctggacccat gaataacttt
     2341 attttggcaa tcgtgttatt catcattctg gcatttatgc aaggtggtgt tcaagtgacg
     2401 aacactaatc gcgtaggaga gatcatgcct aatggagctg cggcagaagc aggcttaaaa
     2461 gaaaatgatg aggtagtcag tgtggacgga aaagaaatcc attcatggaa tgatctgaca
     2521 acagtcatta cgaaaaatcc aggtaaaaca ttagacttta agatcgaacg agaaggtcag
     2581 gtgcaatctg tcgacgtgac accgaaatct gtagagtcaa atggcgaaaa agttggccaa
     2641 cttggtataa aagccccaat gaatacaggt tttatggata agatcattgg agggacgcgt
     2701 caagctttca gcggttcatt ggaaattttc aaagccttag gatcactatt tacaggtttc
     2761 agtttagata aattaggcgg acctgtcatg atgtaccaat tgtcttcaga agcagcaaat
     2821 caaggtgtaa caactgttat tagtttgatg gcgcttcttt caatgaatct cggaatcgtc
     2881 aatctgcttc cgattcctgc cttagatggc gggaaattag tcttgaatat ttttgaaggg
     2941 attcgcggga aaccattaag tcaagaaaaa gaaggaatat tgacattagc tggctttgga
     3001 tttttaatgt tgttgatggt gctagttaca tggaacgata ttcaacgatt ctttttctag
     3061 aaaaagttga caaatagggt ataataacaa acgataacta ttttgaggaa ataaaggagt
     3121 atatagaatg agacaatcaa aaatgttgat tccaacatta cgcgaagttc caaatgatgc
     3181 agaagtatta agccatcaaa tcttgctaag agctggatat atccgccaag tatcagcggg
     3241 tatttattca tatctgccat tagcaaatcg tgtattggaa aaactgaaac gcattatgcg
     3301 ggaagaattt gaaaaaatcg gagcggtaga aatgctgatg cctgccatct tgccagcaga
     3361 actttgggaa gaatccgggc gttacgaaac atatggacca aacttgtatc gtttcaaaga
     3421 ccgcaatgat cgaaaaatga tccttggacc aactcacgaa gaaactttta ctgatttgat
     3481 gcgtaatgaa atcaattcat ataagaaatt acctttaaat ctttatcaaa tccaacctaa
     3541 atatcgtgac gaaaaacgac ctcgcttcgg tttgcttcgc ggaagagagt ttatcatgaa
     3601 agatgcatat tctttccatg caacggaaga aagtttggac gagacatata aagattacga
     3661 aaaagcctat accgaagtat tcaagcgttg tggcttagaa tttcgctcga tcatcggaga
     3721 cggcggcgca atgggcggga aagactcaaa ggaattcatg gcgatctcag aaatcgggga
     3781 ggatacgatc tgttattcga ctgaaagtga ttatgctgca aacttggaga tggctacaag
     3841 ctactatgta tcaaaaaaat cgcatgaaac acagcttgaa ttggagaaag tagctacacc
     3901 taacgtaaaa tcaattgacg aagtagcaga tttcttttct gttgaacctc aaaaaatcat
     3961 caagtctgta ttgttcattg cggatgaaca acctgtcctt gttttggtac gtggagacca
     4021 tgaagtcaat gacgtaaaac tgaaaaactt tttatctgct gactttttag aagaagcaac
     4081 agaagaagaa gcacaaaaat atctaggtgc tgagtttggt tctgtcggac cagtcggtgt
     4141 ttcagatgac gtaaaagtat atgcagaccg ttatgtccaa gatttagcga atgccattac
     4201 tggagcgaac gaaacaggat tccactataa aaatgtgaat ccaaaacgcg atttcactgt
     4261 gctttcttat gaagatcttc gttttgtaca agaaggagat ccttcaccag acaacaacgg
     4321 tgttcttgcc tttactcgag gaatcgaaat cggacatata ttcaaaatcg gcactcgtta
     4381 cagtgaatct atgggagcaa ctgtactaga cgaaaatgga cgagaaaaat ttgtgattat
     4441 gggctgttat ggtatcggtg taagtcgttt attatcagca atcgtagaac aacattcaga
     4501 tgaacaagga atcaattggc caaaaggaat tgcgccattt gatctgcata tcgtccaaat
     4561 gaatctaaaa gacgaatacc aaacaagcct gacgaatgaa ctagaagaag cgatgacaaa
     4621 agctggctat gaagtactgg ttgatgacag aaatgaacgc gcaggagtga aattcgcgga
     4681 ttctgatttg attggctgtc caatcagaat cactgtcggt aaaaaagcag tggatggaat
     4741 cgtagaagtg aaaatcaaga aaactggcga aatggttgaa gtacgtaaag aagaattggc
     4801 gaatacttta ccaatcctct tgaaccaagg cgaataataa gcgttaaaac acagaaaata
     4861 aaaatagctg aatcagctgt ttttgacaat ctgatgattc gcttttaaaa ttggaactgc
     4921 gatttgaatc gcagttccaa ttttttaatg taaaactcag aaaaaacgag tatactaaaa
     4981 ggtactgaaa aagaagagga taagatgcga tcaattgcat tgtttctcat cagaaagggc
     5041 gtatatcttt gtcagaaaaa cgagatttat ttgaaaaact gttggaacaa atccaactag
     5101 aggaagcaga aaaaaatcat ccactgctta ctgctggtga aatggatagt gtagtggtcc
     5161 atcgtaaatc gcgtttatgg gaatttacgc tacatttttc caaaattttg cctattatgc
     5221 tatatcggtc attgactcag catctgacta tcgcttttaa agatattgca caagtcaaac
     5281 tgaatatctt agcagatgac cagacattcg atgaacagct cttgcaagat tactgggccc
     5341 aagctttgga aaatcagcaa tgcgatacac cactagcaca gcaagtattg aaaacgcagg
     5401 tacctgtgat aaaagatcgt aaagtcatct tgccaatcga cagtacagga gctatttctt
     5461 atttgaagca acaatacttg ccattagtag aagagttgtt cgtcagttac ggattcccaa
     5521 aatttcgtat cgaaccagaa gtggatgagc agcaagcgga acgtgtattg aaactttttg
     5581 aagaacgcaa acaagaacag gcagaagcct ttatgaagca agcagctgaa agtctagttg
     5641 tccacgaaca aaagaaaaag gaaagaaaag agcaatcgcc agctcttgaa ggacccatcc
     5701 agctaggacg aaatattcca gccgatgaac caacgacacc aatgatcagt atcgttgaag
     5761 aagaacgaag agtcacactt gaggggtacg tattcgacaa agaagtcaga gaactacgtt
     5821 caaaacgcaa gatcttgact ttgaaaatca cagattatac ttcatcattt atcgtcaaaa
     5881 aattctcgaa cggcgaaaaa gatgaacaag tatttgatgc gatcagtgtc ggtagctggt
     5941 taaaggtacg aggaagtatt caagaagata cgtttgtccg tgatttggtc atgaatgcgc
     6001 aagatatcat agaagtcaaa catacacctc gcaaagatta tgctcctgaa ggggaaaaac
     6061 gagtagagct tcatgttcat agcaatatga gtacgatgga tgcaacaaac agcatctctg
     6121 atcttgtggc acaagcagga aaatggggac accgagctat cgccattaca gaccatggtg
     6181 gtgcgcaagc ctttccggaa gcacatagtg ctggaaaaaa agcaggtgtg aaaatcttat
     6241 acggcgtgga agcgaatgtc gtagatgatg gtgtcccaat tgcttataat gatgcacatg
     6301 aagcattgag tgaagccaca tacgtcgtat ttgacgtgga aacgacagga ttgtctgcag
     6361 tatatgacac gatcatcgag ctagccgcag tcaaaatgta taaaggaaat gtgatcgaaa
     6421 gctttgatga atttatcgat ccaggacatc cattgtcacg aacaacggta gatctgactg
     6481 gtatcacgga tgaaatggtt cgagggtcaa agtcggaaga agaagtattg cggatgttcc
     6541 ttgaattttc aaaagataca attttggtag cccacaacgc ggcttttgat atggggttct
     6601 tgaatactag ctacgcaagg tatggtattc ctgaagcggc caatccagtc attgatacgt
     6661 tggaattagc tagatatctg tatcctcaat tcaaacgttt cggattaggt gtgttatcta
     6721 aaaagtttgg tgttagcttg gaacagcatc accgagcaat ctatgatgct gaagcaactg
     6781 gtcacttagc atggatcttt gtgaaagagg caatggacaa tcataacatg ctttatcatg
     6841 atcagctaaa tgagcatatc ggagaaggag attcttacaa aagggctcga cctttccatg
     6901 taacgatttt agcaaaaaat caggcaggac tgaaagattt gttcaaactg atttcaatgt
     6961 cgaatgtcga atacttcgaa cgcgtcccac gaataccacg ctcccaattg aaaaagatgc
     7021 gtgaaaatct attgatcggc tcagcttgtg ataagggaga aatattcgaa gccatgatgc
     7081 aaaaaggggt agaagaggca agaaaccgag caaaattcta tgattatatt gaagttatgc
     7141 caaaagcggt ctatgcgcct ttgatcgagc aagaattggt aaaaaatgaa catgatttgg
     7201 aagaaattat ccaaaatttg gtagaaatcg gaaaaagttt ggataaaatc gttgttgcaa
     7261 caggtaatgt acattatctg aacgaagaag atgccattta ccgaaaaatt ttgatcaatt
     7321 ctatgggcgg agcgaatccg ttgaatcgtc atagtttgcc ggatgtccat ttccgaacaa
     7381 ccgatgagat gctaactgca tttcactttt taggggaaga aacagcgaaa gagatcgtag
     7441 tagaaaatac gaataaaatc gcagatatct gcgaagaagt gattccagtc aaagatgaac
     7501 tttatacacc gaagattcca ggttcagaag atgaaatatc agaattgagt tacacaaaag
     7561 caaaacaaat gtacggagat ccgttacctg agatcattca aaaacgatta gaaaaagagt
     7621 tgaattctat taatggtaat ggcttctcgg ttatttatct gatcgcccaa aaactagtgc
     7681 ataaaagtaa tgaagatggc tatctagtag gttcacgtgg ctctgttggc tctagctttg
     7741 ttgcgacgat gacaggaatc actgaagtta atccattagc ccctcattat tattgtccag
     7801 agtgccaata ttccgagttt tttgaagatg gtacgtatgg ttctggattt gacatgccag
     7861 aaaaacaatg tccaaaatgt ggtgcacgct taaacaaaga cggtcatgat attccatttg
     7921 agacattttt aggattccat ggagacaaag tgcccgatat tgatttgaac ttctcaggtg
     7981 attatcaagc agaagcccat aactatacga aagtattgtt tggcgaagac tatgtttacc
     8041 gtgccggaac gattggtaca gtcgccgaca aaacagcata tggctatgta aaaggatacg
     8101 aaagggacaa taatctgcag tttcgaagtg cagaagtcga cagattagca aaaggcgcta
     8161 ctggagtcaa acggacaacc ggccagcatc caggggggat catcgttatt cccgattata
     8221 tggatgtcta tgattttacg ccgatccaat atccagcaga cgatcaaaat tcagaatgga
     8281 aaacgactca ttttgatttc cactcgattc acgataatgt attgaaatta gatattctag
     8341 ggcatgatga cccaactgtt attcggatgc ttcaagattt atcagggatc gacccacaaa
     8401 cgatccctac ggatgacccg gaagtgatgc ggatatttgc cggtcctgaa gtacttggag
     8461 taagtcagga acaaatttat tccaaaacag ggacattagg tattccagaa ttcggcactc
     8521 gttttgttcg agggatgtta gaagaaacgc atcctacaac ttttgcagaa ctactgcaaa
     8581 tttccggact atctcatggt acagacgtat ggttaggaaa tgcggaagaa ttgattcgac
     8641 gaggagatgc gacactggcg gaagtaatcg gctgtcgtga tgatattatg gtttatttga
     8701 tccatgctgg attagatagc gggatggcat ttaaaatcat ggaaactgtt cgtaaaggac
     8761 aatggaacaa gatccctgat gaattgcgag aaacctatct atcggcgatg aaagaaaata
     8821 atgtgcccga ctggtatatc gattcttgtt caaaaatcaa atatatgttt ccgaaagccc
     8881 atgctgcagc ttacgtattg atggctcttc gtgtcgctta tttcaaggtt tatttcccta
     8941 ttttgtatta ctgtgcatac ttttctgtac gtgcagacga ctttgatcta gtctccatgt
     9001 gcaaaggaaa agatgctgtg aaacaagcaa tgaaagaaat cacggacaag ggattagatg
     9061 cttccgtaaa agaaaagaat caactgacag tgctggagtt agccaatgaa atgctggaac
     9121 gaggattcaa atttggaatg attgatttgt ataaatcgga tgccgttaac tttgttattg
     9181 aaggtgatac gttgatcgca ccatttagag ctgtcccaag tttgggaacc aacgtggcga
     9241 aacaaatcgt tgaagcaaga aaagacggtc catttttatc caaagaagat cttgccaccc
     9301 gcggtaaagt ttcaaaaaca ttgatcgaat atatgaacga caatggcgta ttgaaagatc
     9361 taccagatga aaatcaatta tcgttattcg atatgctata agcatctgtt ttcctgtctg
     9421 cttgtaattt taagcagagt atggtatagt tcttataggt aatgatacta actgtgagtg
     9481 agcggatttt tcgctcactc tttttaatgg aattacgcac ttaattttta aggaggcgaa
     9541 acttttgagt agcgtcgttg aaacagtcac agaaatggtg acaccgattt tagatgaaca
     9601 aaattttgaa cttgtcgaag tcgaatttgt aaaagaagga aaaagctggt tcttacgagt
     9661 atttattgac aaagaaggcg gtatcgatat cgaagaatgc gcctttgtca gtgagaaact
     9721 gagtgaaaaa cttgatgcag cagatccaga tccgattcct caagcttatt tcctggaggt
     9781 atcctccccg ggagcagagc ggccattgaa aaaagaaagc gactatgaaa aggcacttgg
     9841 tgattatgtc catgtctcct tataccaacc agtagatggc gaaaaacaat atgaaggatt
     9901 tcttcaatca tttgatgctg aacagcttac attgaaaata cgcataaaaa cacgagaaaa
     9961 agaattgact tttgaccgaa aaaatattgc aaaagctcgc ttagctatcc aattttaatc
    10021 acggagagga atcagaaaaa aatgagtaaa gaaatgttaa atgcgttaga cgctttagaa
    10081 gctgaaaaag ggatctcaaa agaaatcgtg atcgatgctt tagaagcagc attggtttct
    10141 gcatacaaac gtcactatgg ccaagcacaa aacgtcgaag tggaatttga tcagaaaaaa
    10201 ggaaagatcc atgtctatgc ggtaaaagaa gtaacggaag aagtgatgga ttcacagttg
    10261 gaagtctcat tgaaagatgc acttttaatc aatccagcat atgagatcgg tgatacgatc
    10321 cgttttgaag taacaccaaa agattttgga cggatcgctg cgcaaacggc taaacaagtt
    10381 attttacagc gtgtacgtga agcagaaaga acgattattt ataacgaatt cagtgcttat
    10441 gaaaaagaca tcatgcaagg aatcgtcgaa cgccaggata aacgttatat ttatgtcaat
    10501 cttggtaaaa ttgaggcagt tctttctaaa caagatcaaa tgccaaatga attttatcaa
    10561 ccacatgatc gaatcaaagt ttatgtttcc cgagtagaaa atacgtctaa aggtccgcaa
    10621 gtatttgtta gccgcagcca tccagaccta ttgcgtcgct tatttgaaca agaagtacca
    10681 gaagtctatg atggaatcgt tgaaattgtc agcatcgccc gtgaagctgg cgatcgttca
    10741 aaagtggcag ttcgctcaaa tgatccaaat atcgatgcag tcggtacatg tgtcgggcca
    10801 aagggacaac gagttcaagc aatcgtcaat gaattaaaag gcgaaaacat ggatatcgtc
    10861 gaatggaatg aagatccagc tgtattcatc gcaaatgcat tgaatcctgc acaagtcatg
    10921 gatgtgatct tcgatgagac aaatccaaag gcctgcacag ttgtcgttcc agactaccaa
    10981 ttatcgctag caatcggtaa acgcggacaa aatgcacgtt tagctgctaa attgactaac
    11041 cataaaatcg atatcaaatc agaatctgac atgacggaat tttatgaaaa tcaagaacaa
    11101 caaaaagaaa cagaagaact gcatgatgaa gcaatcgttc agtcagattt aacagacgat
    11161 gaatatgaaa caattgcatt caacaatgaa acggtcgaag aaaaaccaga agcttaactt
    11221 tagtaaggag gcaaagagat gaaacaaagg aaaattcctt tacgcaaatc cgtggtttca
    11281 ggtgaaatga aacctaaaaa agagatggtc cgaatcaccc gttcaaaaga aggggaagta
    11341 tcgatcgatc caactggaaa aatgcctgga cgaggagcgt atgtctcact tgagccggaa
    11401 gaagtacaac aagcttggga taaaaaaatt ctcgatcgag tactcgaaac aacattaaca
    11461 gatgagtttt atcaagaact tcttgactat gtcactcacc aaaaagcacg taaagagtta
    11521 tttggaaaat gaatacagtt aatcggcaaa aagccatgaa tctcatcggt ctagcgatgc
    11581 gcgcgggaaa actaatcacg ggtgaagaac tgaccattgc tgaaatccgc aaacaaaaag
    11641 caaaattggt gtttgtggca accgatgcaa gtgagaatac aagaaaaaag ataaaagata
    11701 agagttcata ttatgaagtt ccttgctttg agctattttc tgaagcagaa atcacgcaga
    11761 tgatcggaaa acctcgcaag gtattcggaa taatggatgc cggatttgcg aagaagacca
    11821 aggagctaat tgaaggttag gaaggtgatt gcatgggcaa caaaagaatc tatgaattag
    11881 caaaagaact caaaaaatct agtaaagacg tcgtggaaaa agcacaacaa cttggcatcg
    11941 atgtgaaaaa ccatatgggt gcgattacta gcagtgatga gaagaaattg caacaagcgt
    12001 tcaaaaaccc aagcacgaaa caacaagctg cacaacaagc aagcagaaaa ccagccaatc
    12061 aacagccgaa cgatcaacgt aaaaaagaaa caacgaacaa tcaacaaaaa aataaaaata
    12121 accgcagtta tcaagatcgc ggtcaaggga gcggtcaagt gaatcaagga aaaaaaccaa
    12181 caacaaacca aaataatacg ggaaatacca gcagccaaaa tcgtcaagga acgagccaag
    12241 gcggcaatca aaaccgtact aacaataaca acaaaccaaa caataacaat cgcggcaaat
    12301 ttaataataa taaccgtaat cgttataata aaaaaggtaa aaaaggcaaa cagcaacaat
    12361 caactaaacc agctgtacca ccacgcaaat tccgtgaatt gccagatgta ttagagtata
    12421 ctgaaggtat gaacgtagca gatattgcga aaaaaaatca tcgtgagcca gcagaaatca
    12481 ttaaaaaatt atttatgctt ggtgtcatgg tcaatcaaaa ccaagcactg gataaagata
    12541 cgattgaact gttagcgacg gactatggca tggaaccaca agaaaaagtc caagttgaca
    12601 ttgctgatat cgataaattc ttcgaggcag aagaagtcga tccagataaa ttagtctcac
    12661 gtccgccagt agtaacgatt atgggacacg tagaccacgg gaaaacaacc ttattggata
    12721 cattgcgcca ttcacgtgta acaagcggcg aagccggcgg aatcacacag catatcggtg
    12781 cctaccagat cgatattgat ggtaaaccaa tcactttctt ggatacacca ggacatgcgg
    12841 cctttacaag tatgcgtgca cgtggagcaa gtatcacaga tatcacgatc ttagttgttg
    12901 ctgcagatga tggcgtcatg ccacaaacag ttgaagcaat caaccatgcc aaagctgcag
    12961 gcgttccaat catcgtcgct gtaaataaaa ttgataaacc aggtgcaaat cctcagcatg
    13021 ttatgcaaga attaagtgag tatgaattaa ttcctgaagc atggggtgga gaaacgattt
    13081 ttgttgagat ctcagcgaaa ttcggccaaa acatcgaaga attgctggaa atgatcctgt
    13141 tagtcgcaga agtagaagat ttaaaagcag atccaacaca acgcgcaatc ggaacagtga
    13201 tcgaagcacg tcttgataaa ggtaaaggtc ctgtgtcaac cttacttgtc caagaaggaa
    13261 cacttcacgt tggtgaccca atcgttgtcg gtaatactta cggacgtgtc cgtgtaatga
    13321 caaacgatct tggccgtcgc gataaagaag caggacctgc aacgccagta gaaatcactg
    13381 gcttgaatga tgtacctcaa gcaggtgacc gtttcgtagt cttcgaagac gaaaagacag
    13441 cacgtgcagc gggtgaagaa cgcgcaaaac gtgcattatt ggaaaaccgt gctgccacaa
    13501 gccgtgtgac tttggacaac ttatttgaaa gcctgaaaga aggcgaattg aaagaagtta
    13561 acgtaatcat caaagcagat gtacaaggtt cagcagaagc actggctgca tcattgaaga
    13621 agattgatgt ggaaggtgtc cgtgtaaaaa tcgtccattc agctgtcgga gcaatcaatg
    13681 aaagtgatgt aaccttagca gcagccagca acgctattat catcggtttc aacgttcgtc
    13741 ctacaccgca ggcaaaagtg caagctgata ctgaagaagt cgatattcgt cttcaccgta
    13801 tcatctataa agccatcgaa gaaatcgaaa ctgcgatgaa agggatgcta gaccctgaat
    13861 ttgaagaaaa aatcaccggt caaatgatcg ttcgtgaaac cttcaaagtt tctaaagttg
    13921 gtacgattgc aggtgcctat gtaacagaag gatatattcg tagagatagc ggtgtccgtg
    13981 taatccgtga cggaatcgta atctatgaag gccaacttgc aagtctgaaa cgattcaaag
    14041 atgacgtgaa agaagtcaaa atgggttatg aatgtggtgc gatgattgaa aaattcaatg
    14101 acatcaaagt agatgacgta attgaaggat ttatcatgga agaagtcaaa aatgactaaa
    14161 aactgataaa aggaggccaa tcattatggc taactaccgt gatcgccgag tcggtcaaga
    14221 aatcttaaaa gaagtcaatg atattttgcg caaaaaggtc cgtgaccctc gtgtggagaa
    14281 cgtaacgatc acagatgtga aagttacggg ggacttacag caagcaacga tctattacag
    14341 cattttatct gacttagcat cagataagaa aaaagcacaa gatggattga ataaagcatc
    14401 aggattgatt cgtcgagagt tagggcacaa tctaagtatt tataagacac ctgaattgtt
    14461 ctttgaactt gatgaatcag ttgtctatgg gaaccatatc gatgaattgc ttcgcgactt
    14521 gaacaaagac taatcaaaaa atcccgcaaa tcctgtgaaa tcaggggttg cgggattttt
    14581 ttgttttcat taagacgtat tacctaagag ttccatgact ttagtctggt agcgtgtcag
    14641 ctaaccaaaa aagagaaaaa gaaaagtaaa aaattcgact ttcttttttc tatagtatat
    14701 aaatttttgc atatagataa agatcaagat accgataacg gaagctagat tgtaatctat
    14761 tttagtgaca gtcaggtata gccaagtaac tagaagatca gtggaacctg caccataaaa
    14821 gtttgagttt gtcggtccgt tgacagttaa aagaaaaatc acgttgaagt tattgatatt
    14881 tccgattacg ccgcttcgat ttggtcacga tccaaatttt ggataatccc tgtagaaatt
    14941 agcatagtag ctgggatacc aattcacata ttgacaaaga taacagagac ttttacataa
    15001 aacggatcaa tcaggaaagg aattgctcga tccagcccca gttttccaac aatgtgttaa
    15061 ctggcctagc gtcattcagc aaattatgca tgatgaggag cgaaatgaac tgcggaacag
    15121 ccattgtgat aacgacaagt tttttggatc aatgtaaaaa acgtcagcga aaggaaaaat
    15181 ttcgtggttt tttatacctg ttcatttggc tagatataaa atccaaacaa atgaacagaa
    15241 gtgcttatta ggtttcaatt gggagtatct gcgctagaat aagagtaata atgaaaagga
    15301 gtgttatgta tggattattc tagtaaagaa tactttgata aaatgacggc ttggtggcgt
    15361 gctgctaatt atctttctgt tgggcaactt tatcttaaag ataacccctt actgagacgt
    15421 acactgaaac cagaagatgt caaaaaacat ccaatcggtc attggggaac gattcctggc
    15481 caaaatttta tttatgtcca tttgaaccga gtcatcaata aatatgattt gaacatgttt
    15541 tatatcgaag ggccaggaca cggcggacaa gtcatggtat cgaatgctta tttagatgga
    15601 agttacacag agatctatcc agaagtaacg gaagatgaga caggaatgca aaaattattc
    15661 aagcgatttt catttcctgg aggcattgct tctcatgcag caccagaaac accaggttct
    15721 atccatgaag gcggagagct tggatactct ctttcacatg cagtcggagc tgtattagac
    15781 aatcctgaag taatctctgc tgtcgtgatt ggtgatggtg aagcagaaac cggtccttta
    15841 gctggaagct ggttctcaaa tgtctttatc aatccagtga tagatggagc tgttttaccg
    15901 atccttcatt taaatggtgc aaaaatagcc aacccaacga tcttggcacg taaatcagat
    15961 ggagaattag caaattactt taacggactt ggctgggagc ctttctttat tgaaggaaat
    16021 gatccagaaa aattaaatcc tgttatggct gaaaaaatgg atcaagcaat cgagaaaatc
    16081 aaatccatcc aaaaagaagc tcgcctgaag acagcaacag atgtcgtgat gccaaaatgg
    16141 ccagtattga tcgtacgcac accaaaagga tggaccggtc caaaagaatg ggacggggaa
    16201 ccaattgaag gaaccttccg agcacaccaa gtaccgattc ctgtagatca agaacatatg
    16261 gatcatgcag atgctttact ccgttggctg aaatcctatg aaccagaaaa attatttgat
    16321 gcacaaggac gtattttaga agaaatcaga gaaattgcgc ctactggtga ccaaagaatg
    16381 gcaaaaaatc ctatcaccaa tggtggaatc gatccaagac cgcttattat gccagattgg
    16441 aaaaaatata ctttgcaatt tgaaaaacca ggctccgtta aagcagaaga tatgaccgaa
    16501 cttggtaaat tcgttcgcga gatcattgag aaaaatccag aaaatttccg tatatttgga
    16561 ccagatgaaa caaaatcaaa ccgattgaat caagttttca aaacgacaaa tcgccaatgg
    16621 atggaaaaaa tcgaaccaga aaatgatgaa tggctttcgc catctggtcg cgtcatcgac
    16681 agtcaattat cggaacacca agatgaaggg ttcttagaag ggtacgttct gactggacgg
    16741 catggttttt ttgcaagtta cgaatcattc ttgcgagtgg ttgactctat gttgactcag
    16801 cacttcaaat ggatacggaa atcacatgac ctttcttggc gaaaagatta tccttcgctg
    16861 aatctcattg caagctcaac agtcttccag caagatcaca atggatatag ccaccaagat
    16921 ccaggaatat tgacacattt ggcagaaaag aaggcggagt tcatccgtga atatctgcct
    16981 gcggatgcaa atacattgct cgcagtcatg gataaagcat tccgctcgtc agaaaaaatc
    17041 aatttgatca tctcttccaa acatccgcgt gctcaatttt actcagcaga agaagcagcc
    17101 gtcttagtga atgaaggctt gaaaatcatt gattgggctt cgacagcaaa agaggaggaa
    17161 cctgaattgg tcattgctgc tgcaggaacg gaatctaact tagaagcatt agcagctgta
    17221 actttgctgc tggaagaatt cccaaaacta aaaattcggt tcatcaatgt cgtagattta
    17281 ttgaaattac gtcatccaag tcaagatccg cgtgggttga gcgatgaaga atttgatcaa
    17341 tattttacaa aagacaaacc gatcttattt gctttccacg gttatgaaac attggtccgc
    17401 acgattttct ttgatcgtca taaccatcat ttaatgattc atggctacaa agaaaatgga
    17461 gatatcacga caccatttga catgcgtgta gtaaatgaac ttgatcgtta tcatttggca
    17521 aaagacgcag ctttaaaaat caaaggaagt caagcagaag attttgcgga aaaaatggat
    17581 cagaaattac aagaacatca aaactatatt cgtgaaaatg gtattgatct gccagaagta
    17641 ttggattgga aatggaaaaa tcttgaccaa taaaatgata aaatcgatcc cgcaaaacag
    17701 aattttttac attgtattat ttg
//
LOCUS       DAZAFF010000055        17347 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000055 DAZAFF010000000
VERSION     DAZAFF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.055"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..466
                     /locus_tag="ACG9BB_001779"
     CDS             29..466
                     /locus_tag="ACG9BB_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837716.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            561..1445
                     /locus_tag="ACG9BB_001780"
     CDS             561..1445
                     /locus_tag="ACG9BB_001780"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN2837717.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1669..4836
                     /locus_tag="ACG9BB_001781"
     CDS             1669..4836
                     /locus_tag="ACG9BB_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFN2837718.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4904..5401
                     /locus_tag="ACG9BB_001782"
     CDS             4904..5401
                     /locus_tag="ACG9BB_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFN2837719.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5420..5884
                     /locus_tag="ACG9BB_001783"
                     /pseudo
     CDS             5420..5884
                     /locus_tag="ACG9BB_001783"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5881..6639
                     /locus_tag="ACG9BB_001784"
     CDS             5881..6639
                     /locus_tag="ACG9BB_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFN2837720.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6725..7465)
                     /locus_tag="ACG9BB_001785"
     CDS             complement(6725..7465)
                     /locus_tag="ACG9BB_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN2837721.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7526..8779
                     /locus_tag="ACG9BB_001786"
     CDS             7526..8779
                     /locus_tag="ACG9BB_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN2837722.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9101..9328
                     /locus_tag="ACG9BB_001787"
     CDS             9101..9328
                     /locus_tag="ACG9BB_001787"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFN2837723.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9451..9777
                     /locus_tag="ACG9BB_001788"
     CDS             9451..9777
                     /locus_tag="ACG9BB_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFN2837724.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9845..10558
                     /locus_tag="ACG9BB_001789"
     CDS             9845..10558
                     /locus_tag="ACG9BB_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFN2837725.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10868..11718
                     /gene="licT"
                     /locus_tag="ACG9BB_001790"
                     /pseudo
     CDS             10868..11718
                     /gene="licT"
                     /locus_tag="ACG9BB_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            11839..13704
                     /locus_tag="ACG9BB_001791"
     CDS             11839..13704
                     /locus_tag="ACG9BB_001791"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFN2837726.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13718..15161
                     /locus_tag="ACG9BB_001792"
                     /pseudo
     CDS             13718..15161
                     /locus_tag="ACG9BB_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            15269..15970
                     /locus_tag="ACG9BB_001793"
     CDS             15269..15970
                     /locus_tag="ACG9BB_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2837727.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16096..16608
                     /locus_tag="ACG9BB_001794"
                     /pseudo
     CDS             16096..16608
                     /locus_tag="ACG9BB_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(17006..17215)
                     /locus_tag="ACG9BB_001795"
     CDS             complement(17006..17215)
                     /locus_tag="ACG9BB_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFN2837728.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         5791 a         2910 c         3725 g         4921 t
ORIGIN      
        1 atcaaaaaat gcacgtagaa gtagataaat ggagtggttt tgcaaatttc ttttatttct
       61 agaagttatt cacgtatacg gatttgtttt tctcacaaat aaaaaggcag gaagtacaat
      121 ggatcatttg tatataaaat atattcatca attttccgtt ttggtgttag gcacactaat
      181 tatagcgggg attgtcgcat ttattattca aggccgtgac atattaacag tacatcaaga
      241 gtttttcagt tatgcatttg gtattcctct gatcgctata aggtctgata acaaaaagtg
      301 gaagaaacgc tattacattc tcttgctatt tttgtgtttt tatctgccac ctaagatttt
      361 tactttatta ggtatacaat gcctatcatt ttcttttatt tcctatatca ttgttttaat
      421 tttcctcagt ttcatagtat ggatgtttta tcttgtcaaa aaataaatga atgtttcatg
      481 aacagtactt catcacagcc taattttttt ctttatgcta tcgttatatt aaagaaatca
      541 atcagaaggg aaagaaagtg atgaaaaaag ttttattagt ggtaaacccc agttcaggcg
      601 gtgaacaagc aaaggaattt gagcaattag cgatagcaaa attggaatcg gtgtttgatg
      661 aagtggtcgt cttgcataca aaaaaagcag gggatgcaaa aaactttact cgcgaagcgg
      721 ctacggaagg gtatcatagt gtgtttgtca tgggcggaga tggaacagtc aacgaaggaa
      781 tcagtggaat cgctgaacaa gaacatcggc caaatttcgg atttttccca ttgggtacgg
      841 tgaatgactt ggctcgagca ttagggatac cgctagaacc tgaggaagcg atcaatcatt
      901 tttcgattga atcagtcaag ccattggata ttggaaaaat aaatgacgac tattttatga
      961 atgtcgtcgc aattggctct atccctgaat caatcaatga tgtagatgcg gagaagaaga
     1021 cgaaatttgg aaaactagct tatttcatgt caggaatcaa acagttagct tccacacaat
     1081 cctactcgtt tcacgtagaa gttgatggaa agaaggaaga aattgagagc agtaccttgc
     1141 tgataggact aactaactca gtcggcggtt ttgaaacatt acttccaaat gcaaaagtgg
     1201 atgatggtaa gctccatttg gtttatttaa aagattcttc tttgatcgat acgcttaaaa
     1261 cattgccaga cctattaaaa ggagtagatg aatctacgaa aaatctagcc taccttactt
     1321 gtgagcaatt agttgtgtca ttagctgatg aggaagaatt gacgacgaat gttgatggag
     1381 atgaagggga taaattacct gtgacaatca aagtattgcc ctcacattta caggtttatt
     1441 gttaaaatgg aggttgtgac cgaggagttg gcgggagaac accgcttgtt cgggataagg
     1501 gactgtgaca agacttttgt cacagccttt tttttgtaca aaaaagaaat ttacacctaa
     1561 tagaaaaatt tttccataaa aatacatatt ttaaatatga aagtacaatt ggaaatagat
     1621 taaaacgata aaataaaatt gtcaaaacgt cagagaggat ggtggagaat gaatcaatgt
     1681 gtaaaaaagc tgattgcgtc gattttgttt gtcttccctc tcttgatggt gacatccgtt
     1741 tttgccgatg tagagaaaac aaacgaatca acgcaatcag agaatcaaag ttttgacaag
     1801 actacttttt cagaagaatc gattacttct gaaactacag aatctcaaag tgaggcttct
     1861 actaataaaa ggcaagaaga aaataagaca agtgagacct cagaagtagc tgaacaaacg
     1921 tctgatagca gtgaagcggc actggcaaga gctgtttcag cggatgggta cagcgaagct
     1981 gccacaacag aagaactggc gcagcttctc gcagacgaat cagtaactaa gatccgactg
     2041 attcagcctt tgactttaga cagggaatta gagatcaaac gagatatcgt tattgatttt
     2101 ggtggattcg ctcacaattt cggcacacac catatttata tcaatgagat acctcagtcg
     2161 atcgagttcc agaattttaa aggaacagct gcacatccag gtggattgac accaaatatc
     2221 gatggaaatg ccattatcat tgcttatatg accgattttt ggggaagcag atatcatttt
     2281 acgggagaaa taaaatttac cgggacactt gatttgtatg atgggtccaa attaggactg
     2341 atttacgctc ctcgtgcaac tgtcacatta gatggtgttt ccggtgtgtt ggatgtccaa
     2401 ccagtgaagg aaggactgaa tgccgctccg ggtaaagcct atttttgtag aagctatcaa
     2461 ttgaatgtga tcaatggtag tcaactttat ggaccgtatc tagggaaatt ctacggtttt
     2521 ctagattcag gagatgagac tggtagtgga aaaacagcgc caggaatcca tatcttaagc
     2581 ggttcaaagg tctctcttga ttatgatcgg gcagatggcg ttagcgatga aggagaggca
     2641 gtcgatacgc tacctggtaa tgtcatgttt gaagtcagtg gttcgggaag cgaattttcg
     2701 gtaaatacaa agatcaatat taccaatgac aacaatcgcg gaatcatcca aatgagaggt
     2761 tcaggatcga aagtcactgt atcgaatggt gggaaaatca ccatcaatac agcaactacg
     2821 ggcggatttc gcttgcaagg agattctagt caaatccatg tatcaagtgg tggacaaatc
     2881 tcggtcaaaa tggctggaga tgacgatcaa cccgggaaca atgggatgcg ttttgtagga
     2941 aaaggccttt ctttgatcgt agaaggagcc ggatcagtta tcgacatcga taagcagagt
     3001 gggcgttcca gcgcagttcg ttttgagaat ggtacgcaaa agctgacagt aaccaatgga
     3061 ggctcattga gggtaagaaa tacaggtgat ggcaaatctt acgtgaacag actcaatcaa
     3121 ggaaatcaag cgatccaatt ttatgacgct tctggttttc tagaaagtcc aggttcagct
     3181 gattttacag tcactggaga aaaatcaaat atcgatatac gcgcagattc cggagcaact
     3241 gtagatacaa ctggagatat tgatttaaat tttacggcag gtgaaaaaac ttatttagtc
     3301 atggtcggga aaacagggga agataagacc ggcatcatca gtggggatgt gttgaatgtg
     3361 gacttagaat ctcctaccta ttttgatttc caaaacaaac ggattggaag cgagaccaat
     3421 ggcataggcg gctgggtatt ccaaggaaat ggagattctt ctttaaagat cgcccattca
     3481 gaaatcgctt tatggcgaaa aacaggcgca acattcgata actttgaaaa agacccggat
     3541 tatttttctg atatgacaga cttatcgatc aatgggaccg acttaggcaa tttcgtatca
     3601 agccctagtg caggtttaac aaatgaattt gctcagacag gtgccggaat gaaaaattat
     3661 aatcgaatca gtgccaataa tcagctcccg atgattgaat ccttaagagt gccaacgaat
     3721 gcagataaaa aaatctatgg gcatgtcgtt gttcccgaag gtgtcgaggc aaagccaaga
     3781 gatgcttgga caggcgaagt agaagtgact ttacgtatta cttacgcaga tacgacgaaa
     3841 tcatctgtag aaattactgc tctcacgcaa ggacaaacag atgaaaatga aggggcagga
     3901 tataacccat ggggagaagg cgaacaaggc ggcttgttcg aagttcaagt acccaatgat
     3961 gagagattaa aaacggagga tcaagtgcaa gttgttgctg ctaaaaaagt aagaggtggc
     4021 caagaagtga cagaactgga ctctgccaaa acgacagtcg atgtgattcc tcctgaacct
     4081 gctaatttaa gtaccacatt gatcaatgca gcttctcgca cgatcacagg tacaggtgaa
     4141 gcaggagcct tggtttcatt gaaaaaaaat caagctgttt tgtcagaaac aactattgat
     4201 gatacagggg aatttgttct ttctatccca gaaaatcaac tttttgcagg agatgtatta
     4261 gaaattgtat taaacgacca agcaggagaa gctactgcta agggtgtgca gaacaagcca
     4321 agtaccaacg atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat
     4381 gccccgcctt ttgcacctgc tacgaagatc gtagtagaag gtggtctctc atttcttgcc
     4441 ccttccaaac tcgattttgg tcagatcaaa gcaactggtt tgacacaaga agcatttggc
     4501 caaatggata tacaggaaaa attatggatt tatgatcagc gagaaacgaa aaatccgtgg
     4561 atcttaacga taaaattatc tcagcctttc acaaatgatg caggattttc tatgcaggat
     4621 gtcctatatt tcaagaaagg agaggagtat caattagtga atgaagaaga actggaaatt
     4681 gtcagaggac aaaattcaac gaatgtgcaa gcagattatt cagaatattt aaataacggt
     4741 cgggcgttta aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag
     4801 ataacatgga cattagcaga tgcgccgaca gaataaagat caaggaggat gattgatgaa
     4861 tcgaatatgg aataaaagtc atcgaaacaa ctgctcttta cttatgatcc ttctccttgg
     4921 attcatcctt tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga
     4981 attaccggct aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg
     5041 gaaaacacag gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga
     5101 gacattgatc cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa
     5161 aagtaaattt gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga
     5221 aataactgtt cctcctcaaa gtgcggtcac tgagacactt tcactgaaag cgccagaaaa
     5281 agagctggct ggtgtggtcg caggagggat cacgtttcaa gaaaaaaaag agaacgtttc
     5341 taacaaaagt acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgatttg
     5401 aaatagcgaa aaagagtctt ccccagattt gaaattaaaa aaagcttatg cggatcaaag
     5461 cgatggaaga aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg
     5521 cttgactaca tcggttgaaa tctacaaaaa agaaacaaaa gagccttacc tttcaataaa
     5581 aaaagaaaaa atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg
     5641 aaaaaaatta gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg
     5701 ctggaatctc cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa
     5761 ggatattgat ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg
     5821 agtcattctt attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa
     5881 atgaaaaaaa caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa
     5941 agtgtttttg ctgagaataa gagctcagcc actacagaag gaacgatttc tgtgaccaaa
     6001 ccagatgctg caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatcct
     6061 gatactcctt ttacacctga cgaaccaaat cagggaacat ccggattact gacgattgac
     6121 caagctccag atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct
     6181 gcattacaaa gcggaacgga cagtactgga gcacaaacac aagtacagaa tttcgtacaa
     6241 gtaacggata aaagcggaaa ttatgccggt tggaaattat cagtcaaacg aacagaattt
     6301 attggtacaa catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc
     6361 gtgattcgaa atagcgcaac taatagcagt ggaatgccat ctaccgtttc tggaactggc
     6421 gctgaaggaa tcgaaatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat
     6481 gaagggattg gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca
     6541 gtggaattat ttgttcctgt atccaattat gctgtagaca actacgtttc aacattcaca
     6601 tggtcgttaa cagatgctcc tacagaagag gaagcgtaaa atcatatcaa cgatttcagg
     6661 gagtggagcc gattcaaggc ccactccctg aaatctgcta tgcttacgtc tcagctctta
     6721 ttcatcaact cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga
     6781 tctttcgacc aacgagtaag gagtcaccgt tgaaaaattc taacacataa tgagcagaat
     6841 caattttctg caattgcgat aagttgatac agtaagattt taaaagtaaa aagtaagcat
     6901 acttttctaa ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag
     6961 ttgttttgac cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc
     7021 gaggatacag gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga
     7081 tctgctgttc tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc
     7141 tgtcttttct gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat
     7201 tcgtataagc ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg
     7261 cgacatcaat acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat
     7321 tatcaatagt gcccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga
     7381 tattctcgat atgatgattg accaattctc tgatgatcca ttcgctgtga aactggttat
     7441 cttctatgat aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt
     7501 tgaaacaaaa caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac
     7561 tttgatttta gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag
     7621 ctttggttgt atagctttga tggtggtgac acttttactt cctatcccta aagtgagctt
     7681 tccttaccta gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt
     7741 tatcagctgt ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt
     7801 ttttgatctg atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact
     7861 acttatcgca tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct
     7921 caacttgctt tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag
     7981 ctggggcgtg atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct
     8041 tctatctctt ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat
     8101 cagtttccaa gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga
     8161 agaatattac catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt
     8221 aaaaattact cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tattagaaga
     8281 tcattctgca gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc
     8341 agctgtcagg ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat
     8401 ccaagtagtt attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg
     8461 ctgtttatct atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat
     8521 tacgatcaat ctgctggaag aaaaagaggg atcacgtttt agtatagcga acaaggcaaa
     8581 tccaacgaat ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg
     8641 cgggttaggt ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa
     8701 aatcgaattt tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa
     8761 gagcaaatca gcctactgac ttactgtcaa aggataagtt tgaggaaagt ctacacggag
     8821 atagagacaa caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct
     8881 tctgtttctt ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag
     8941 tacgaaagta tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg
     9001 tcaaaatgct aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta
     9061 ggcgtggaga agcaagcaaa tccctacgat cacaaaaaaa atgctgaccc agcagttaag
     9121 agagttagaa caagatgaaa gtatccatcg aaaagcctat aaccaagtcc cgcctaaagt
     9181 agagtactcg ctcaccgaag aaggaaaatc attaagagag attctagtag cgttgtctgt
     9241 ttgggaagaa gaacgtatcg aaaaacaaaa agcctcaagg gaagaagaca cgctactgca
     9301 tacacatggt ggctacctaa attattgatt ttctatgaca tttattttaa ataaaagaaa
     9361 acaggagttt ttttaaaagc atcatctatt ttttatgtaa aaaaatggta tgattaatca
     9421 agtaagaagt gattggaaga aggcaagcaa atgaatagta tggaaaaagt caaccagttt
     9481 agagatgaac gtaattggcg acaatttcat aacgaaaaag atttggctat ctctatttct
     9541 ttggaagcat ctgaattgtt agaacttttt caatggaaaa cgccggaaga agtaaaagaa
     9601 acacaattag aacgaatcaa agaagaactg gcagatgtat tgatctattc atacatgatg
     9661 gcagacaacc ttcacctgga tctagatgag atcattgaag aaaaattgat caaaaataat
     9721 ttgaaatatc ctgtagaaaa aagcgcaggt gtacgaaaaa aatacacaga gttgtagaaa
     9781 tgtgtcattc ggctgttgga aagagcaaaa gaaaaacagt aaaatgagtc tggagggaga
     9841 gaaaatgaaa aaaaaaggaa aagcaaaaag atggctagtc aatatcctct tgttcctctt
     9901 gttattagta ggactagctt tgattttcaa tgaacagatc aaggattatt ttgtcagaga
     9961 gacaggagat aaatatgcta tagcgaatgt aacaaaagaa gatctgaaaa agaataacga
    10021 taaagatgtc agttttgact ttgatgcggt agaaccaatg acgacagaag gggtcatgag
    10081 gtcgcaaatg agaggaacag atctaccagt gattgctagt atcgcagtcc cttcagtttc
    10141 aatcaattta ccggttttca aaggcttgga taatacgtca ctactttacg gagcaggaac
    10201 attatctcca gatcaagaaa tgggaaaagg aaattatgcc ttagcaagcc atcgtgcaac
    10261 gaaccctgaa ttgctgttta caccgctaga aaatttagag atgggtgcca agatctattt
    10321 gacagattta gaaaatgtgt atacatacaa aacatttttt aaagaaaaag ttgctcctac
    10381 agatacccaa ttactgaatg aagtcgaggg aaaagaaatt gtcacactaa ttacttgtgg
    10441 cgacatggac gccgtcacgc gactagttgt acaaggagaa ctggaaagtg ttacatcgat
    10501 aaaagatgcg acagatgata tgagatctgc tttcaatcta gagacaaaaa cattttagga
    10561 tactgggaca taagtcgtta cagattaaaa acagccaaag gatatgaatc gataaggaaa
    10621 aaccaatttg ctgaaaaaaa gagaaacgca aagaggtgca tctctgattt ggttgttttc
    10681 tagatagact tgtgttccag tttttttatt ttttatatga aaaggcttgc ataaataaaa
    10741 attcgatggt atgatgattt tgaataagaa atggattgtt actgtacggc aggcaaaacc
    10801 agaattcagc taagaaacag taggcgaatt ctaggttttt ttatttttct tgagagggcg
    10861 aagcaaaatg aaaattgata aaattttgaa taacaatgtc gtcatttcga aaaatggatt
    10921 cggggaagaa gtcgtttgta tgggaaaagg actagctttt caaaaaaaga ttggggacga
    10981 gatttcgcca gaagcggtcc aaaaagaatt tgtttaagag attcttttgc taaaaaccaa
    11041 ttccagcagc tgatggctga tattccggca gaggagatcg aatgggtcaa acaagtagtc
    11101 gaactggctg aagataaact aaaagttgaa ttttcgccta atatttatct gacattgacc
    11161 gatcatcttc attatgcgat cacacgagca aaagaaaata tccaactgcc taatccatta
    11221 ttatttgaaa caaaaaaatt ttatccgaaa gaatatcaag cagcgaaaga ggctcttttg
    11281 ttcgtgaaac aaaagacggg tgtggaactt tctgaggatg aagccggttt tattgccttt
    11341 cattttgtga atagccaaca aggaaacgaa gacatgcaag tgacgatgac cgctaccaca
    11401 atggtaagag atatactgag tatcatcagt aaattcttcg gcatcgtgtt cgatgaagaa
    11461 tctttgaatt atcagcgtat cattactcat ctgcagtttt ttgcccagcg ttatctcaaa
    11521 ggggaaggat ctgacgagca agatgaattt ctctacgcat tagttcaagg aaagtatcca
    11581 aaagcgtttc attgtgtcga acggatcaat gagtatcttc gacaaacgca aaaacaggaa
    11641 atggggatcg cagagcagat ttatttaacg attcatattc aaagagtagt aagtgagaaa
    11701 aaaagcatcc aatcataagg attgttactg tatggcaggc aaaacctgga ttgaaaaaat
    11761 aggttcacga atgtttgtga ctgttttttt caatctgggt ttttattttt taaataaaaa
    11821 agaaagaagg aaaaaacaat ggataatcaa gcagttggtc gtcgtgtatg ggaagcggta
    11881 ggaggacaaa agaatgtcaa aagtttagta cattgtgcta cgcggttgcg attcagatta
    11941 aaagatgaat ctttagcaga tacgcaaaag ttaaaagaag acccggacgt gatccaagtc
    12001 gtacaaagtg gcggtcagta tcaagtagtg attggcagta atgtagcaga tgtttatcaa
    12061 tcgatcgtgg atgaggaagg tcttgcagtt tcggaggatg gaaaagaaga gtccaaaaat
    12121 attttgaatc gtctgatcga tattatctct agtatattta caccttttct aggggcgatg
    12181 gcagccgccg gggtattgaa aggattctta tcattggcaa cggtgatggg ctggctgaca
    12241 gctgatagcg gtacttatca aatattgttt gctgcagcag atggtgtctt taccttctta
    12301 cctgtcatgc ttgcttttac agcagctaaa aagttcaaag ccaaccagtt tttagcagta
    12361 gcaattgcta tggcacttgt gtatccagct atcacagcag ttgctggtgc gggagaagct
    12421 atcagtttct ttgggatccc agtgattctt tcaccatctg gttatacttc ttctgtcata
    12481 ccaatcattc tagccgtatg ggttcaaagt aaattagagc cattcgtaaa aaaagtgatt
    12541 ccgcaatttc tgcaaatgat tttagttccg cttgtcgttt tagtagtgat ggttccatta
    12601 acatttcttg ctttaggacc aatcgggacg gttgccggta atgctttagg cggtctcttc
    12661 aactcgattt atggatttag tccaatcgta gcaggtctaa tcatgggaag tttatggcaa
    12721 gtattcgtga tgttcggcat gcattgggga ttcgtaccga tcatgttttt gaatatcgaa
    12781 caatatgggt ttgatgtatt gatgccaatg cttcttccag ccatcttggc acaaggtggg
    12841 gctgctttag ctgtagcatt gcgaacaaaa gatacgaaac ttcgagcatt agggatttct
    12901 tctacagtga cttcgttatt tggaatcact gaaccaactg tgtatggtgt gacattacct
    12961 ttgaagaaac cattcattgc cgcttgtatt tctggaggta ttggtggagc tattatcgga
    13021 ttttccggag taaaagcttt ctccagcagt ttggtcagct tattgacgat tccgactttc
    13081 attaatacgg tggatggtgt agagtcaaat gtgacagtag cagtaatagc tacaggaatt
    13141 gcttttgttc ttgcattcgt aggtacattg attttaggat ttgacgaaca aacacaagac
    13201 aatcaattag aaaacaaaca tgcgaatgca ggagaaccga ttacttcagc acggcatacc
    13261 ttaaaaagtc ctttaacggg taaagtactg ccattgagtg aagtaccaga ccaagttttt
    13321 tcttctggcg tgatgggaaa aggtatcgct attgatccag aagtaggaga actagttgca
    13381 cctgcagacg gagagatcac gactattttt ccaactggac atgctgtagg gatcacaacg
    13441 actgacggag cggagatttt gattcatatc gggatggata cagtagaatt aaatgggaat
    13501 ggctttgaga tcttagtcaa acaaggggat ctcgtcaaag caggagattt attgattcgt
    13561 tttgacatcg aagcaattag agcagctggc tatagcgtaa tcactccagt tgtcattacg
    13621 aatactgatg catttgccga tatacttgaa ttagaccaaa aagagattat tgcaaacgaa
    13681 gatgttctgg caattgtaaa ataaggagga aatcaggatg caatcacgtt tttcagaaaa
    13741 atttttatgg ggtggagcaa ctgctgccaa atcaattaga aggggcttat gatcgagacg
    13801 gaaaaggact atccgttgca gatgctatgc ctggaggtaa gcagcgcttt gcgattatcg
    13861 gcagtgaaga atttgactgg acgattgatc aagaaaaata tatttacccg aatcatcgag
    13921 gaatcgatca ttacgatcgt ttcaaagaag atcttgcctt gtttgcggaa atgggattca
    13981 aatgttatcg tttttcgatt gcctggacac gaatttttcc aaatggagac gaaggtactc
    14041 ctaacgaagc gggattagaa ttttatgatc agctgattga tgagtgctta aaatatgata
    14101 ttgaacctgt gatcacgatt tctcattacg aaatgcctct tcatttggca aaagaatacg
    14161 gtggctggaa aaatcgaaaa ctgatcgact tctatgaacg atttgctcag acagtattag
    14221 agagatacag cagtaaagtc aaatattgga tgactttcaa tgaaatcaat tcggcctttc
    14281 actttccagc tttaagtcaa ggattagtaa aaagcaatgg agcaggagag taccaaaata
    14341 ttttccaagc atggcataat caattcgttg caagtagcaa agccgtaaaa atcggccacg
    14401 aattgcgttc ggatatccaa atcggctgca tgattatcta tgcaacaact tacagcatcg
    14461 atgccaatcc agtcaatcag gcagcaacaa tgatccaaaa ccaagaattc aattttttct
    14521 gcacggatgt ccaagtacgt ggagaatacc ctgcatacac agcgcgtacc cataaaaaat
    14581 atggagtcga tccggaaaaa ttagagcaaa cggaaaaaga cttccagttg ctaaaagaat
    14641 atccagtgga ctacatcgga tttagttatt atatgtctac ggcaatcaat gagactgatc
    14701 cacaagcagc aacttcagaa gggaacttgc tgggaggagt aaaaaatccg tttcttgagg
    14761 caagtgaatg gggctggcag atcgatccag aagggctgag aatcgcattg aatgaattat
    14821 acaatcgtta ccagaaacca ttattcattg ttgaaaacgg tcttggagcc attgatcata
    14881 tggaagagga tggcacgatt attgatgatt atcggatcga ttatttacgt cgtcatatcg
    14941 aagcgatggc taatgcagta gcagacggtg tagatttgat gggctatacg ccttggggat
    15001 gtatcgatct tgtcagtgca tcaacaggag aaatgagtaa acgttacggc tttatctatg
    15061 tggatctaga cgatgaagga aatgggacac tgaatcgatc aaagaaaaaa tcttttgatt
    15121 ggtataaaga agtaattgcc tcaaatggcg taaacttgta attaaagcag ctgaatgaaa
    15181 aagaacacac ttctcacatg tgatttgtgt tcttttttta ttttttatgt gctattcttt
    15241 tagtaatagt aaagcaaagg gtgacgttat gaaaaggaca cgtgtactat acttggaagt
    15301 cgcagataaa attaaagaag atattttctc aggaaaatat ccagtcggtt cgatgttgcc
    15361 gacagaaagt gaattagaag aactgtttca agtcagcaaa atcactgtcc gaaaggcaat
    15421 cgaactgctg gcatcagatg aatatgtcgc aaagaaaagt ggtcgtggga cgacggttct
    15481 aagcaatcgt ccttacaatc gattatccaa agcggcaagc tttacgcaaa tcttggagaa
    15541 ctcccgatat gaagtccgga aagaaacgct ggatttgcaa ttggtccata taaagaagga
    15601 agatcctttg ttttcctttt tcggtgagca agctgtttgt tttaagcgct tatattattt
    15661 gaacaatcag ccttatattt attttgttca ttatcttcca caagaaatga tgcaattcag
    15721 cagagaagaa tttggcaaag aatctttgta tcgtttgctg acgaaaaaag gatatactat
    15781 tgactcattt tctgatgatt tctcggcagt atttttaaca gaagaagaac aaggaattct
    15841 gaaaacagac gcacagatcg gcatcaaacg gatacggaaa tcgtctacgc aaattgggga
    15901 aatgattgaa tattcggagg cggtctacca tacggagctg catccttacc atattgatta
    15961 tgaaacataa ataaaaaaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
    16021 gccaaacaaa aatagcttct catatttttc gtttggttag gcttattacc gcagatacac
    16081 ggctgtttct ggattaccat ttattctgga taagggggtg tggcacgatt ttcagaagga
    16141 atctatgaac caatcactgt attgatttta gcttcgatgg taaccccatt cggcttgacg
    16201 atcgcttatt tcttagggaa aatcattcgt aaaaatatac tcaatcgtca agaaatcgat
    16261 acattgaaga ccgcttttcc tatggggatc tgtcagatca cagagggatg tttccctatc
    16321 gtattgaacg acttggcacg caatgtcata gcaacgggag tcggtgggac agtaggtggc
    16381 ggattaagca tgttttgggg cgcagatagc cgtattccgg catcgggaat gtttgcagtc
    16441 gctacgatga ccagaccatg ggcattcatt ggtgcattgt tagctggttc ttttgtcaca
    16501 ggaataacca ttttattatt gaaaaaacca gtagatccga atgcagaaat catccaagaa
    16561 gaaaaagagg aagaagacat atcttgggat gatttgacaa ttagttagga gaggcagaat
    16621 ggattacaag aaaaaagtac aatagatgta ccttgagaca ggaatggtat gaaagaatca
    16681 gagttagaaa gaatcgaata tacagatttt ggtttaggag cgatcgaaac tgtccagagc
    16741 atcggcaccc tccttgaaat gacgatgaag gaaaaatgag aacctttcgt tgtcgaaagg
    16801 gtctggtata ccttatgtcg aaggaaagga aacaaaaaaa ctttcctgta ctattccaaa
    16861 aaggaaagaa agttattata cagtaaaaaa agaaattatt cttcatgcta gagatcagta
    16921 cacgttcgag cccaatatca aacactagtt ccaggcaggc gcaacaggtg cggttatcac
    16981 tgaattttct tccagcagtg acgattcaag tgatattttt acaaatccag ctatttatcg
    17041 ttaacaaaaa gatttttagg agaagctgat gaacaaaaat gatcaaattt tgatcgttta
    17101 gttcgtcggc ttttttattt agagaaacat catttatcag tgaaacataa cacgagaaca
    17161 aaatgatttt ttcgttaata aaagaaacaa gtatttgttg tttaaaataa tacacgttat
    17221 tggcatatag gacgaaatcc cagtatcttc tataagtaag tcatttcata tcgatgaaaa
    17281 cacgttattt atttttgttt tatctataaa atggctgaaa aatgattttt ttctaaatac
    17341 tatatat
//
LOCUS       DAZAFF010000056        17345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000056 DAZAFF010000000
VERSION     DAZAFF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.056"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..586)
                     /locus_tag="ACG9BB_001796"
     CDS             complement(41..586)
                     /locus_tag="ACG9BB_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFN2837729.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(598..1290)
                     /locus_tag="ACG9BB_001797"
     CDS             complement(598..1290)
                     /locus_tag="ACG9BB_001797"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFN2837730.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1309..1593)
                     /gene="macP"
                     /locus_tag="ACG9BB_001798"
     CDS             complement(1309..1593)
                     /gene="macP"
                     /locus_tag="ACG9BB_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFN2837731.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1606..2169)
                     /locus_tag="ACG9BB_001799"
     CDS             complement(1606..2169)
                     /locus_tag="ACG9BB_001799"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFN2837732.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2397..3059
                     /locus_tag="ACG9BB_001800"
     CDS             2397..3059
                     /locus_tag="ACG9BB_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFN2837733.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3183..4379
                     /locus_tag="ACG9BB_001801"
     CDS             3183..4379
                     /locus_tag="ACG9BB_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFN2837734.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4480..5856)
                     /locus_tag="ACG9BB_001802"
     CDS             complement(4480..5856)
                     /locus_tag="ACG9BB_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN2837735.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KVFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5893..6760)
                     /locus_tag="ACG9BB_001803"
                     /pseudo
     CDS             complement(5893..6760)
                     /locus_tag="ACG9BB_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(6849..7874)
                     /locus_tag="ACG9BB_001804"
     CDS             complement(6849..7874)
                     /locus_tag="ACG9BB_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFN2837736.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7892..8566)
                     /locus_tag="ACG9BB_001805"
     CDS             complement(7892..8566)
                     /locus_tag="ACG9BB_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN2837737.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8750..9643)
                     /locus_tag="ACG9BB_001806"
     CDS             complement(8750..9643)
                     /locus_tag="ACG9BB_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFN2837738.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9717..11414)
                     /locus_tag="ACG9BB_001807"
     CDS             complement(9717..11414)
                     /locus_tag="ACG9BB_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFN2837739.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11407..12720)
                     /locus_tag="ACG9BB_001808"
     CDS             complement(11407..12720)
                     /locus_tag="ACG9BB_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFN2837740.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12720..16661)
                     /locus_tag="ACG9BB_001809"
     CDS             complement(12720..16661)
                     /locus_tag="ACG9BB_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN2837741.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16770..17228)
                     /locus_tag="ACG9BB_001810"
     CDS             complement(16770..17228)
                     /locus_tag="ACG9BB_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN2837742.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4860 a         3897 c         3014 g         5574 t
ORIGIN      
        1 ttattttgtg ctttttattt tatcaaatca ctttgttttt ttataaaatt tctgcgccta
       61 gtgtggactc aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg
      121 aatctttgaa aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca
      181 cacagatatc tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac
      241 ggatgtctag atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat
      301 tttcctcttg ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac
      361 ccgttcctat cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg
      421 gatcatgcgc atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat
      481 gagcagtcag ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct
      541 ctgcaacaaa atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta
      601 tacgagatgt ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat
      661 aaattcgtca aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat
      721 caaaaatggg atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca
      781 agctaatgct tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa
      841 tgtatcacct gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt
      901 aaccatttca ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg
      961 tcctgggttg tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat
     1021 aacgatgtca cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac
     1081 catgttcacg ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc
     1141 tataccggaa cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc
     1201 gcctgcgcgt tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc
     1261 ttccattgcg ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc
     1321 gattgctaaa aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa
     1381 agagttccat ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt
     1441 ctttttgctt tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt
     1501 ttctgctttt cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag
     1561 ttcgcttcgc gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt
     1621 caatagttcc cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt
     1681 catcgcttgt tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc
     1741 ttgtgctaaa ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc
     1801 atttgcaaaa cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata
     1861 gccagtttct tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg
     1921 atcgattttt ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt
     1981 gacaagaagc agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa
     2041 taccagttct ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc
     2101 aataattttt ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt
     2161 gttcagcata tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa
     2221 tttttctcaa aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat
     2281 agaaaatcct tctttagtcc gatactcttt ctagtagtcc gtatgctaaa ataagtatgt
     2341 aatcgaataa cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga
     2401 taaaacgaaa ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca
     2461 atagtttttc ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac
     2521 tctttggttc gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac
     2581 ttgaaactca ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa
     2641 caatgaatca aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca
     2701 aacttaaagc aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag
     2761 aattggtgaa agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc
     2821 cgaatctagt tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca
     2881 aacagaccta tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa
     2941 tcgcacaaga ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat
     3001 ctgttgcgaa acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac
     3061 tcttttgttc ataaaagaag actcggaaca ttcagatgat gtctggctgt ttcgagtact
     3121 ttttctaaag tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt
     3181 ttatggatca taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag
     3241 atttattgaa caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg
     3301 agattgatca gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg
     3361 aacgccaaga acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg
     3421 tcatcagcta cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga
     3481 atcatgtgca agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc
     3541 gtctccaaga agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg
     3601 tcttcgggaa ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg
     3661 cacaaattga caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata
     3721 atctgatgtt agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt
     3781 atatcgctgc tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga
     3841 tgaaaagagc agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc
     3901 aattcctaga tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga
     3961 tccagcaagc accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga
     4021 tccaagcatc tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga
     4081 ccttgcttcg tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg
     4141 acttattgaa gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa
     4201 atgaacgtgg aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa
     4261 caatccaaga gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag
     4321 tagagcttgg acacatggaa gaagatctga aacaaaaact gttggattta acccaataga
     4381 tcacctttcg cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg
     4441 gcgtttttct tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct
     4501 gtgtccatag ccatagccaa aataaatgat cagtccaata accattacga taccgaacac
     4561 gatccatgtc acagtttgca gacgaagcat caatacaata caagtcaaaa tggagacgat
     4621 tggcaatagc ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacacttt
     4681 tctcaacttc agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt
     4741 tagttccgct aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc
     4801 ggcataagtc gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg
     4861 aagtaagcca tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat
     4921 cgttaccgtc agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg
     4981 tcctacatac ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac
     5041 gattcctgtc aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc
     5101 aatgattcct cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac
     5161 ggcatcaaat cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa
     5221 accaaaaggc atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat
     5281 aaacaacgcg atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt
     5341 cttggcttca agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc
     5401 aatgtatgta ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc
     5461 taaacttttc aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc
     5521 tagtaaaaat tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac
     5581 aacatacaga taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa
     5641 acttagtcca gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg
     5701 accagcattt tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc
     5761 accaattcca agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg
     5821 attgagattg atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt
     5881 ggaaaacagc gattaaactg ttttcatata ccctaaaagg gataccacct aaaatgacta
     5941 aaaggcaacc aacaataaca taaatgagct cttttttcgt ttttctttcg ccaaggatga
     6001 aaatcccacc taaagtagag ataatgatcc ccatttgtga aagtgagaag ccaacagcta
     6061 aaccaagtga tttgactgtt agaagcatgc agatgtttcc taagccccat aataatccac
     6121 agatcatgtt tttccacaca aactgatcga ctttaacttt acggaaagca aagaagcttg
     6181 cgcctaagat cattccgata ctttgaggta agataattga catcgcatcc aaattcgccc
     6241 agttgacgat gatggtatat acaccataac caatcgtaga gaagatcaat gctctaaaac
     6301 ccttaccaaa atcaagcatt ttattatctg tatctatttt tccttcgtca tcttgtcggg
     6361 cagtcaaata cgcaccgctt acaagaagag ctaaagcaat aataccgtat acataatctt
     6421 tcgtttgtgt ccattcatgg aagaaaacgg ctcccgcaat cgtattcaaa attaattgaa
     6481 aaccagtaga caatggtagc cctacagaaa cacccatgta cttcattcca tggaactgtc
     6541 cattttgtcc aacactccaa gctaagccgg ataaaaatcc gatcaagaaa atccaagggg
     6601 tgattacagg actaacaacg aaaaagacaa ctaaagaaaa cagaaatgca ccgatcgtca
     6661 ttcctagtgt ttgttgattg gcatcgccac caattttacc acttactaaa ccaatactac
     6721 cccatgccaa catagggact aatgctatta aaatttccat aaccaatctc cttttatttc
     6781 acatgataag aaacttatca gcgaaaacgt taacaacaca agcacttttt ttcgtttcta
     6841 acgattttct aacgtttttc attataaaaa tttaagattg ggaaaaataa agtcattgtt
     6901 gtcccttttc cttcagttga ttcagccgtc aaatggatac ctaatttttc ggccagattt
     6961 tttgctaaat aaagtcctaa tcctgttgaa tgtgttttgc taagtcgacc atttctccca
     7021 gtaaatccct tatcaaagat ccgtttcaag tcttccgctg ggataccgat ccctgaatct
     7081 ttaactgaaa ggctgactcc ttgcgcttcc ttttctatgc tcacaatgat gtttccgtga
     7141 tccggcgtat atttgatggc attactaagc aattggttga agataaaagc gatccattta
     7201 gcatcggtca aaacagtatg atcctcacca acgatctcgt agcgaatatt tttttggata
     7261 aaataatttg cctgactgcg tatcacaggc tgaatgattc cttttagtcc atattcttgg
     7321 atgaggtagt cccgcgagaa actgtcgagt cttgcataat acaaaacttg ttctacgtat
     7381 tcatcgattt ttgataattc attttctagt aatgtatact tttgatcgtc aatatcgaac
     7441 tcgatcgaac gaactagcaa tttagaagcg gaaagtggca ctttgatttc atggacccat
     7501 gagtcaatat aatctttttg gtcttgttga ttactgattg ctgattgcat tacttcttgg
     7561 tgttcacgga tcagctgatt gatatattct tctaccagtt tttcttcttc tgtcctccca
     7621 ccactcaaat aattttgcaa aggggaagtt ttttgtatgt cggctaattt tccccaccat
     7681 cttctcttgc tcaagtaacc acccagcaga taaaaaatca gaaagacgcc ctcaatcaaa
     7741 gctaaataag caattgtgct caaatcaact ttgatgtttg gcgccagcca catcacaaag
     7801 atcgtcaatg caataaaaaa cagccatccc aagatcaatg tccactgatc tttcaaatat
     7861 ttaaaaaaat tcatcttaac cgctcatcct ttcagggaac gatatatccc tgtcctactt
     7921 ttgtttcgat atatccttgg atacctgcct gttcgatttt tcggcgtaac cgattgatgt
     7981 tgactgtcaa tgtattgtca tctacaaatc gttcgtcatc ccacagtgct cgcagcaatt
     8041 tttcccgact taagatcttt ccattgtgct tcatcaaaat aaataataga cgatattcat
     8101 ttttgctcaa atcaatgatc tctccgttga tttccatact tccgttgtca acattcaacg
     8161 taatgccatt gtggctcatc atttcactag ataattctgt gtagttgtaa gaacggcgaa
     8221 gtaatgcatt gatttttgca atcagtacat cgatttggaa atgttttgtc acaaaatcat
     8281 ctgcccccat gttcatagcc atgatcatat ccatattcgt attcctgctg gagataaaaa
     8341 tgatcggtac tttcgatact tcacgtattt tttgacacca ataatagcca tccaaaacgg
     8401 gaagattaat atctagtaat acaagctgtg gttcttctct ttcaaagtct gagaacactt
     8461 gattgaaatc ggtcgttcca aaggtttcaa attgccattt ctcaagttcc tccatgatca
     8521 attgtcgaat gatctcttcg tcttcgacta ccatgatttt tgccataaaa aatccctcca
     8581 tctttcttct tgctactaca atatcaaaga agcccttgtg aaacaagttg gcttacctgg
     8641 aaaatgtcat tttagcaaac gttaaaaaaa actggaaata tgcgatttaa tcgcgtttgt
     8701 ctcttccaag cagcttaaat ttattatgtt attcttaata attggtctat tatctgttat
     8761 ttttctttcg ttgtttttgt cttttaaatt ttcgataaca ccaataaccg aaaagcgtta
     8821 gcactagcaa tatacctaga aggagtataa gtaatcgata aagatggtat ttttttgtct
     8881 gttctatctt tgaagagact tcttctgtaa agggaactcg tcttcctgtc actagtaatc
     8941 ggtgagtgtt cacggtgtaa ggtgtacaag tgataagtgt gactagatcc tgcttttctc
     9001 taattgctaa tgtatttgtt tttgttggaa ggacaacaat tttctcaacg acttcataag
     9061 cgagcatccg accatacact tcaagataga atcggtcatc ttgctccatt tcttcaagat
     9121 ccgtaaagag ttttttctca gcaataccgc tatgggcggt aatcacggaa tgtgtactct
     9181 ctcctccagt tggaaaagag gtgccttgca ggactgtcgc tcctctatct aacaaaagat
     9241 cattcgtttc atcaaataaa ggcaagctaa cattgatttt cggaatatag atggctccga
     9301 tcatatgttg ttcgtaatat tcttttcctg gattttggac atttctaacc gcttgctcaa
     9361 aaggatcttt gacttgtccc attcctggga ttactgatgt atgagcaagc gtctgatttt
     9421 tcttttcatg cactgctaat tgttgctcat gtttttcttc tttatcgcgt tcaagctgct
     9481 tttgataatt ctcgatcctt ctttgatcca aataattgct gagagcatct gctaaaaatg
     9541 gatatgtaaa aactgcagct ccggcaagaa acatcactgc cataaataat tgtaataaaa
     9601 gactgatttg ggtttgaggt ctattaccta ttttcttttt cattttcttc accttcttat
     9661 ttaaaaaaac tgcagcaacc tttataaaac cgattgctgc agcttatttt tgttgcttat
     9721 acttcagact tcattttaga tttacggtac cagctgtacg ctccaatcat caaacttatt
     9781 ccgattagta agaatgccaa gagcccgttc ccaccagtag caggaagtga cccgcgtttc
     9841 atattcggta ctctttctgg ccctggattt tctactccta cgatctgaat ttcttcatta
     9901 tagcttccat aagagatgat aaattcagta aaggctgcct cacctgttgg aagtacataa
     9961 ccttctggcg ccatggtctc tttcatttga taggttccat aatctaaccc ttgaacctca
    10021 aagtatccat ttccatctgc gacaaattct gttgcttgtc cgcttgttgc ccactcaatt
    10081 ctatctgctc ctgattcata aacagctaaa ggcgactccc ctttataaaa tattgcgtat
    10141 tcagaacggt cttcattcca caattcgaat cttgcatctt taagcaattc actgcttgat
    10201 gcatcatatt tttcaaactt tcttccacct gttgtgactt tctctggcgg ctctactcct
    10261 tcttcataat caaattcatc atttcgtccg atttcaataa ccgctgtgtt gttatggaaa
    10321 tcatccgctt ctagttctgt ggaaaccttc aaattatacg tgatcgttaa atctcctcca
    10381 cgtgcatctg ctaatctacc taaagtatct actttggcta ctccattttc ttctgtcaaa
    10441 atcgtcacga caaacccgtt gcttactact tctactatat aatcttgtcc ttttgttaaa
    10501 atcgtctcgc cggctcgcac ttctatttcc tgatcataat aagttagacc aactgtaggc
    10561 gtatctgtga ttttgtaatt tttgataaca gcgcttccgt tttcaaggac agaatcgatg
    10621 aaataaggaa taggtgctgt gatcgtgtaa gaagccgttt ttcctctttc tagatcaata
    10681 taagaaatcg tttcttgttc tccagcttca ttaataatcg ttacttgtct aaggtcgctt
    10741 tcctctacat tcatctgttt atctgcttct ctgatttcgt ttttcggata caaatgcaca
    10801 tctgtattgt atgtgtcttt ccatgtagaa ccatcccaca cgccatccac catatcaggc
    10861 atcatcactg gcatcataac aaccattgga gacgcgattc tggaaatatg agctggcgaa
    10921 taagtttcta agaaaagata gactttatct ctagaagaag gtgcttcttc cgtcacttgg
    10981 acggtagaga ataccgcttc tccagcttca ttcgttgttt gtttgtcaat acttgtcagt
    11041 cgatatttta aaaaccaact ttctgtgtgc cgtttttgaa tccattcaat cgcttcggca
    11101 gaattaagac cgtcttctcg ttccggtgtt aaaggattat ctcgaatcaa atcgtaatat
    11161 acttccgtta cgtcaaacaa atcaaaatcg actcctggaa tattttcttc accaaaatcc
    11221 gtagaaacca atccgctatt cggtcgttct tccggaacag atgagaaacc ttttttgtgt
    11281 aatgtaatcg tcacttcact tggtttttgt gcgacttctt cggctttgac tagttttccg
    11341 ttcccgaaaa tccctattcc cgcagtaacg agtaacagta cactcattcc cgtgacaatc
    11401 atttttttat tcattttttt ctccccgttc tttttttatt aataaaacag cacctaacaa
    11461 taaaagaatt cctagtaaag aaatcaacgt tcttgcttca ttcgtttgcg ggaatacgga
    11521 ccattttcct gattgacctg gattgcttgg accactcggc tctttcggtt tgtccgttgt
    11581 ttcttttacg gtctcgttat aaacacgtgg tgttgcggat tcataggcag agactggaac
    11641 tttcccggat tctaattcag ccatctcctg tccgttaact actacatatt gtggttggcc
    11701 ttttgcatcc acccaaaatt cagggatgat gacctcgatt ctcttacttg cttcatccat
    11761 ctcgtaacct tccacacctt ttaattcttc aaaataatag gtgccagaag gcaagaaacg
    11821 ttgtcctgtc gtaacaaggc cctctttatc cgaaatgaat ttgcttacat ttttgtcatt
    11881 caaaggatca ttgtctgctg gtttgatcca ttgatttttc aagtcattag caggagccat
    11941 atctaaataa tatttccccc cttctatcat ctgatataga gcaaaaacag caccttctaa
    12001 tggttttcct ttttcagttg ttcctctttg acgtccatac ttgaagaagt atggatctct
    12061 tagataaccg acatttttag gatagatatg aatctcttgc agttcctcgg ttgattctgt
    12121 cgggtgataa attggcaaaa tagcagcaat tgggatagct gtctgatcga tatcaacatc
    12181 caatccaact tctggatcaa tcgattcttc gaaaatcaaa taaactgcat cccgatcttg
    12241 gtttttctta ggtgctttga tacgtgcaat cccgtctcct aatccgccaa ccgtatcttt
    12301 cccagttgtt accgaagtcc ctatttggac aagcctttct tctgtgatta gctgacggat
    12361 attgtttctg ttcgtttcag cgatttgctg gacgaattct ttaggagtca tgttttcagc
    12421 agcttttttt tcatgataat aatcggtcat gtcgtaaatg gcaaatgtcg ctccatttag
    12481 tggcaccgtc tgtgaaatga tatctgctgt ttcccctgct tcctcagaga tagccaagcc
    12541 atcattttca tggtattcaa attgatcgtt ataatcgata tctcgaatca tccgtttatg
    12601 caacacgaat tccacgacat cttcccctga ttcttctgcc gacatcgcat gtggaagaag
    12661 gactgctgca agcggtagta aaacaagtaa ttgaagcatc aaacacaaca gctttttcat
    12721 taccttgctc cctccttttt agattgtctg acaaacaaac cataggctgc tcctgcaagc
    12781 gccatgaaga atgcagcaag caggatatag aataatcgtc cggatcctcc tgtagcaggc
    12841 aacggatttt caggatcgtt cccgacacta agctgtaatc tgattcgatt tgtctccaaa
    12901 agttcatatt ctaactcttg ctgtagatca ggcgtctctg cttcttcgtt tggataatac
    12961 gaaatctcaa cttttagtgt acccgcttct tttcctggaa ctacccgatc attttctatg
    13021 tcaggttctt ctcgataact aatttctaaa ataaagtaac ctggaagttt tctatagctg
    13081 tcaggtgact tcacttcctt gatccgatag accccttcca acaagtctga agcattgaat
    13141 agggaagaat cgtttggatc tggtgccaag cctccctcga tgatttcata ctctcctgca
    13201 ctattttgtt tctcaattga aaattctgcg ccttcaagac gtcggctatt catactgtca
    13261 attttcaaaa gttccaaatc gattggcgct agttcatctt ctaaggttac ttgtagtaca
    13321 ccatcgatga tttcatagcc ttccggcaag ggagcagttt cgtcttcgat gaccgtatct
    13381 tcaatgataa acttaccttc ttcattcagt gcaaactgga acacttgatc agatagccga
    13441 tacccatttg gtgcgacaga ttccgagacc tcatattcac caatagacag atgcagaggt
    13501 tggtcgtaat ttaaatcgac atacttttct actcctacgg cacgagaata gaagtatccg
    13561 atcccaggca aacgtttcga ttcttctggt tctaacggat taccagtgta tgctcgatac
    13621 agcgggaagg aagaatcccc cactttcctg atatcgaaac ctgcaccatc taatgtatgg
    13681 ccttcatggt ctaattttct aactcggatc tcccgataat caaacgtatt gtaagcagtg
    13741 aatgctaccc ataaacgatc ctcttcatct gttgtatacg tgagatattg ccaattttct
    13801 tcacctgttt gcttgagttg gatagtccct atagcattat cgtaaagtcg caagatccat
    13861 gtgtcatttt catcaggttc tttgaaacct tctgggttct ggctctcaat gattttgtat
    13921 tctgcatagt tggtagtttc ttcactatcc ttataaacaa atgaatctgg ctctccgatt
    13981 ggatgaataa ctttctcacc atccacgatt acatatttcc cgtcttcatc tactgaatag
    14041 aacgtgagtc tccctttctc ttcctctact tcgttcacac cagaaagccc tttcgctact
    14101 ggatatccga tttccagtcc gttaccatct gtttcataaa gttcaaactc tgcgccattg
    14161 attggcatat tactgccttc aattagttta ttgatattca tgtggattgg cttgaattca
    14221 tttcgcattt ccagtttgac atacaactca tcatactctg tgccttctat gttaggctct
    14281 tgtacaacag aaagcttgtg atattctcct tcataatagg aattcggatc tttaccttcc
    14341 ggtccgccga ctctcgaacc agatgtatat tcatctacaa gctcgtagtg acctaacatc
    14401 tcatcttcgt tggctgcata ttttttgatt tggaattgat acgtctcatc tactaactga
    14461 taacgtggtg gtccagcgat ctctctgatc caaaatatgt cgctgactgc tccagtttca
    14521 tctcctaaaa tcggcatctt cgtttcaaag atcgctattc cgtccgtggt agttttactt
    14581 gtttcgattg gttcggcttc cccactctct ggattttggt catataatgc aaattcgact
    14641 ccagccaacg gctcgccatt ttgacccttc ttgctgactt ggaaattacg gcctttatgt
    14701 tcgtaacgaa tggaagaaga tgggaaatac atccgtccag tagtgatatc attctcataa
    14761 tcctccaggt tctgaatata ggccatattg ttcactgtgc aaaaatttcc gtcttgagct
    14821 cccgcatcaa gttttacttg gtacttgatc ctgaccattt ccccttcacc taaagaaatc
    14881 ggagaaaatt tataagcttg gttttcccgt ccgacatacg ttgtcggagt cgccactact
    14941 tctggtgggg cttgtccatg acgttcttca tcccatattt ctggctcttg gtacttcatg
    15001 ccgttttctc ctggatctag atggtaaaac tgaatatctg ccgatctgcc agctccaaat
    15061 ggtgagacat taaaaccagt ttcatcataa agtgtggctt gctggatcgt tctcttgaag
    15121 gtagaagaga tatcctccaa ggctgtaaca atgtcattct tgttgttcgc attgtaatat
    15181 cgcggtgtta cgccatcttc ttctaaatca gcaatatttt tcaatacatt tcgtccaata
    15241 gcatctagtc gttgtctccc gctaacgttt ccatcgattc ctaaaccaat ggagaagata
    15301 cttggcgggt tctcttgata cttgtttttc agccaatgat gataagcaac tgtgttgatc
    15361 gcagatgact tttcttgcga agtaggactg ccattcgtat tgttattaga agaaccatca
    15421 ccaacccagt gagtcgcttt attatctcct tcttttacct ctccataacg atagtgtaca
    15481 tttacttcat cttctggacg gttaaagtca gatgcatagg taactggatg agtaaaccct
    15541 gcattagaag tattcccaga aaactcctca aaatttctga acaaaacgtc attatcttca
    15601 gcaatcttat cttccatcac agtccagttg ctccaagctc cattagtgct cgtacgataa
    15661 cttgactgaa tcggtgcata actgaaggtc ggtgtcccat ctcccacaac gattaggatt
    15721 ttttctggat ttctattttc tccgccattg tctttataca atgtttcata gccattttta
    15781 agccctagtg taagcggagt tcctccgatt ggtgtacgat ttaatgctgt attctcactt
    15841 cccgtcaaac gattgatatc atttgtcaat ggaaagatgt ccgaactgat ttgctcatgg
    15901 ttattgatag ctgtactatt atggtaaaaa ttcaccatac cgattcgaat atccagactc
    15961 gggtcagaga gcagatcttt actcatttcg tttactgcct caatcaacgc agcattttta
    16021 gtttgactat tggtcccagc ggttaattca ctcatactcg ctgacttatc tagaacaaaa
    16081 acgatatcga ttggactgat ttcctttttc tcgcccccga tgacatctag atagacattg
    16141 taaagggttg ttgcatcttc aggatgtaga tcagatgctg tgctgcttgg cagaacaggt
    16201 tctacccatt ttttgatgta cgccccgtta taatccaagt aactgttttc aaaattccgt
    16261 tcatttcccc aaagctgaac ggtttgagct ggaacgtttc tatcttcttc gcctgcagtt
    16321 gatccattat tagtagcata gtcgtaattt cgccagttcg ctcccccgtt tgtataatca
    16381 ctatcatcga tcaagttctc tccgtcatat ggcgagcctt tgtctccttg atagcgttca
    16441 ttgctttgtg tccaatttcc taacactttt gtatagtcat tcgttgtaaa cttcgggtag
    16501 cgatgctgtt cgttttcaac ttctccgcta ggatagatat attcataggg gtcttgcgta
    16561 gctgttggcc caagacgctc caaaataccg ttgaataact ggcgttgaat gcgctggagt
    16621 gcttgaccgc tgggtacgat tggttcgtcc agcttttcca tctcattttc ttcgctacgt
    16681 tgaatttcag cggaatcttc tgtatcagtt tcattttctt gtaattcttg gttatctatt
    16741 ctttcatcaa ccaccacttg aagaggtagt tacccttcgt tcacatcggg gatcttttct
    16801 gtttcaaaaa tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc gacataccat
    16861 tcttgaccat cgatcgtctc gacttttagg tcttctccat cttcatcata ggaggataga
    16921 cctgttccac gaacgttttt tacggtacca atcccggcgg ctgccgcatc gattgctaaa
    16981 cgtattctac cttcattggc actttccttt ttttcaaaag ccagatgcca ttcaagggtt
    17041 tcttcttttt caatatactt cccttcaatt tttgcatagt cctcatctaa gaatacctca
    17101 tcctcctttg tttctgttga ctcagcagct acatccaggg tcgtcatatg atagactgac
    17161 tggaaatatg gtccaaatag caaactgaca gttactaact gcaaaagctt caattttaac
    17221 cctttcacat ccttatactc ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa
    17281 acaatgccca tctaattata atttaattta attttaattt taaatcaaat gatatccttt
    17341 ttagt
//
LOCUS       DAZAFF010000057        17226 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000057 DAZAFF010000000
VERSION     DAZAFF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.057"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..371
                     /locus_tag="ACG9BB_001811"
     CDS             3..371
                     /locus_tag="ACG9BB_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFN2837743.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="ACG9BB_001812"
     CDS             551..1492
                     /locus_tag="ACG9BB_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFN2837744.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="ACG9BB_001813"
     CDS             1561..2292
                     /locus_tag="ACG9BB_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN2837745.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="ACG9BB_001814"
     CDS             2436..4616
                     /locus_tag="ACG9BB_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFN2837746.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="ACG9BB_001815"
     CDS             4636..5118
                     /locus_tag="ACG9BB_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFN2837747.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="ACG9BB_001816"
     CDS             5134..5895
                     /locus_tag="ACG9BB_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFN2837748.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /gene="murC"
                     /locus_tag="ACG9BB_001817"
     CDS             6026..7360
                     /gene="murC"
                     /locus_tag="ACG9BB_001817"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFN2837749.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="ACG9BB_001818"
     CDS             7667..8152
                     /locus_tag="ACG9BB_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFN2837750.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="ACG9BB_001819"
     CDS             8145..8828
                     /locus_tag="ACG9BB_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFN2837751.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="ACG9BB_001820"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="ACG9BB_001820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFN2837752.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="ACG9BB_001821"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="ACG9BB_001821"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFN2837753.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /gene="coaE"
                     /locus_tag="ACG9BB_001822"
     CDS             12444..13073
                     /gene="coaE"
                     /locus_tag="ACG9BB_001822"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFN2837754.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="ACG9BB_001823"
     CDS             13125..13457
                     /locus_tag="ACG9BB_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFN2837755.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG9BB_001824"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="ACG9BB_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFN2837756.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="ACG9BB_001825"
     CDS             14283..15656
                     /locus_tag="ACG9BB_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFN2837757.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG9BB_001826"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="ACG9BB_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFN2837758.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="ACG9BB_001827"
     CDS             16711..17151
                     /locus_tag="ACG9BB_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFN2837759.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5875 a         3009 c         3796 g         4546 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 cattaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatggg
//
LOCUS       DAZAFF010000058        16714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000058 DAZAFF010000000
VERSION     DAZAFF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.058"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..1462)
                     /locus_tag="ACG9BB_001828"
     CDS             complement(251..1462)
                     /locus_tag="ACG9BB_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2837760.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1581..2216)
                     /locus_tag="ACG9BB_001829"
     CDS             complement(1581..2216)
                     /locus_tag="ACG9BB_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFN2837761.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2352..3005)
                     /locus_tag="ACG9BB_001830"
     CDS             complement(2352..3005)
                     /locus_tag="ACG9BB_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837762.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(3108..3314)
                     /locus_tag="ACG9BB_001831"
     CDS             complement(3108..3314)
                     /locus_tag="ACG9BB_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFN2837763.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3390..4136)
                     /locus_tag="ACG9BB_001832"
     CDS             complement(3390..4136)
                     /locus_tag="ACG9BB_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFN2837764.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4296..4520
                     /locus_tag="ACG9BB_001833"
     CDS             4296..4520
                     /locus_tag="ACG9BB_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HFN2837765.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            4544..5305
                     /locus_tag="ACG9BB_001834"
     CDS             4544..5305
                     /locus_tag="ACG9BB_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFN2837766.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5317..5574
                     /locus_tag="ACG9BB_001835"
     CDS             5317..5574
                     /locus_tag="ACG9BB_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837767.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5666..5917
                     /locus_tag="ACG9BB_001836"
     CDS             5666..5917
                     /locus_tag="ACG9BB_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFN2837768.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5917..6066
                     /locus_tag="ACG9BB_001837"
     CDS             5917..6066
                     /locus_tag="ACG9BB_001837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837769.1"
                     /translation="MFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDEAEYRAKKP
                     TEGAD"
     gene            complement(6180..6587)
                     /locus_tag="ACG9BB_001838"
     CDS             complement(6180..6587)
                     /locus_tag="ACG9BB_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837770.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6748..6972
                     /locus_tag="ACG9BB_001839"
     CDS             6748..6972
                     /locus_tag="ACG9BB_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837771.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            6982..7176
                     /locus_tag="ACG9BB_001840"
     CDS             6982..7176
                     /locus_tag="ACG9BB_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837772.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7179..8201
                     /locus_tag="ACG9BB_001841"
     CDS             7179..8201
                     /locus_tag="ACG9BB_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFN2837773.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8164..8979
                     /locus_tag="ACG9BB_001842"
     CDS             8164..8979
                     /locus_tag="ACG9BB_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFN2837774.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            8997..9779
                     /locus_tag="ACG9BB_001843"
     CDS             8997..9779
                     /locus_tag="ACG9BB_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837775.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9779..10627
                     /locus_tag="ACG9BB_001844"
     CDS             9779..10627
                     /locus_tag="ACG9BB_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN2837776.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10624..10866
                     /locus_tag="ACG9BB_001845"
     CDS             10624..10866
                     /locus_tag="ACG9BB_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837777.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11009..11377
                     /locus_tag="ACG9BB_001846"
     CDS             11009..11377
                     /locus_tag="ACG9BB_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFN2837778.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11392..11553
                     /locus_tag="ACG9BB_001847"
     CDS             11392..11553
                     /locus_tag="ACG9BB_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN2837779.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11550..11876
                     /locus_tag="ACG9BB_001848"
     CDS             11550..11876
                     /locus_tag="ACG9BB_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837780.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11873..12688
                     /locus_tag="ACG9BB_001849"
     CDS             11873..12688
                     /locus_tag="ACG9BB_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFN2837781.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12827..13138
                     /locus_tag="ACG9BB_001850"
     CDS             12827..13138
                     /locus_tag="ACG9BB_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFN2837782.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13135..13380
                     /locus_tag="ACG9BB_001851"
     CDS             13135..13380
                     /locus_tag="ACG9BB_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837783.1"
                     /translation="MIKKLIQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13377..13793
                     /locus_tag="ACG9BB_001852"
     CDS             13377..13793
                     /locus_tag="ACG9BB_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFN2837784.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13790..14128
                     /locus_tag="ACG9BB_001853"
     CDS             13790..14128
                     /locus_tag="ACG9BB_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837785.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14125..14355
                     /locus_tag="ACG9BB_001854"
     CDS             14125..14355
                     /locus_tag="ACG9BB_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837786.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14362..14565
                     /locus_tag="ACG9BB_001855"
     CDS             14362..14565
                     /locus_tag="ACG9BB_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFN2837787.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14562..14858
                     /locus_tag="ACG9BB_001856"
     CDS             14562..14858
                     /locus_tag="ACG9BB_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837788.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14935..15348
                     /locus_tag="ACG9BB_001857"
     CDS             14935..15348
                     /locus_tag="ACG9BB_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFN2837789.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15361..15747
                     /locus_tag="ACG9BB_001858"
     CDS             15361..15747
                     /locus_tag="ACG9BB_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837790.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16298..16525
                     /locus_tag="ACG9BB_001859"
     CDS             16298..16525
                     /locus_tag="ACG9BB_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837791.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16522..>16714
                     /locus_tag="ACG9BB_001860"
     CDS             16522..>16714
                     /locus_tag="ACG9BB_001860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837792.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         5664 a         2726 c         3485 g         4839 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtt
      121 cgttatttaa tggtatttta aatgaagata agcgaattta cagcattttt aaatgcttat
      181 ataccgcaaa ttatcccaag tttttgtata tcctacccga gtttataccc gaaaatataa
      241 ttttttagtg ttattttata tcttttttta ataggttttc aaacgctgta gttgctgtat
      301 ctttttcttt gcgtaagaca tggccataaa cattcattgt agtattaaaa tctcggtgtc
      361 ccaatcgttt ttgaagctct tttggagtca tattaggatc gctaagcaaa taggtagctg
      421 atgtatgtct aatgtcgtga aaacgaatgt gacgcaaatt gtgacgtttg gtgaatcgtt
      481 tgaacttctt atacagataa cttccacgtg gcaaagttcc atcttgtttt gtatcaaata
      541 taaaatagtg ttttaatttc tttatattta atttccaacg aatcttttta ttctctttga
      601 ttaattcatc taacatatca aatagataag caggaccggt tactattttt tcatcatcat
      661 catttttcaa acctggcagc aaccgaatat ctgattttcc atccacttct gaaatccgct
      721 gatgaaatct tatttcttgt ttgtcaaagt caatatcttt ttcttctaaa gctgcaattt
      781 ctccttgtct tgctcctgta ataaatgcta gaacaattaa agttttaatt tctatgcttt
      841 cctcaaatgc tgcttttagc attgcatgaa tttcttcttc cgaataaggt tcttcgatat
      901 tcttttttac gcctttttct tttggtattt ttacattttc cacaggattt tctttaattg
      961 ctccgtactc atttttagcc atcaaaaata agttgttaac ggcgtataac atacgaagct
     1021 ttgtatttct tgataaaggt ttatcagagt ttctcccagg atcttttgta ttgattcttt
     1081 gagcattaac aacaattttc tttatcatgt atggtttgat atcgattaga gggataaaat
     1141 caaatttatc aagaaatcta ttctcgatca ttcgcttata gttatgataa gactttggct
     1201 ctaaattttt ttcggcctct gaaagccata tttttctata aaattcacca aaagttatat
     1261 tgaacaaatc caacgaatca tcggtcattc cttcgaattg ctctaaccag atgttcaatt
     1321 ctttatatgc cgctctttta ctacttgctt ttatagtttt gctttttctt tttgccgagc
     1381 catcaggttt gtatcctaat acagctctta gtctatatgt ttcgttgtct acttgttcta
     1441 agtatcctgt tacttctgac ataattgaat cagctctctt tctctgatac aataggtact
     1501 ataaagaagc ctatcgtata ggtttgtttc tttcttagaa cacgctcgct ttggtcggtg
     1561 gggcgtgttt tttatactaa ttagttgtga agagcaattg aaactgtctc tgttccaaag
     1621 taacctgttt gtgcttgcaa tacattattt tttgcatttg cttgggcttc agagatatca
     1681 aaaactattt taccagtttg ttgcatatca ggatttaagc tttccataaa gaaagagtta
     1741 gtgatattac cattttcatc ttgatttgcc gacatagatg cagccgaatc agcttcgaaa
     1801 gtttttccat ctgcttttaa tttgaagaat gagctatcaa cagtcacagc cttatcacct
     1861 gcatttttta cagataagtc aactactaag aaagtgtctt ttgcgttagt aggaagtaca
     1921 gatgggccaa cttgttttac tacttcaacg gagtttactt tgtattccat tttccctaca
     1981 gcaacgtcat caccgatttt ataagtcttt tcttcttttg ttgtagtttc ttttgtagtt
     2041 tctttattag tacttgttga tgttgcagta gctgaatctt tgttttcacc gccacttaga
     2101 gcaccaccaa taccaaaaaa caaaagtact attaataccc aaaaccatac ccgtttataa
     2161 aacggttttt taaccttata cattttccca tcttgaccca taactttttt tgccatttaa
     2221 atattcctca tttcttgtta taatatattt gtgatctcag aaatgaggta tgagtccgtg
     2281 ttgcagcacg ggctttttta tttataactt ttttagagat tataggcaaa atagtagggc
     2341 ataaaaatat attattgaat tccgtattta aaaaatccta attgaacttc accggaagtc
     2401 ttttgctgtg ttgtacgcaa tgcttcttct tcagacattc cattctgtac tttccatgca
     2461 acaggcgaca tcccgtattt gttaacaaaa tcagtaagtg ataaagtgtc agcgtcttgc
     2521 tgagcgcttg tttgttggtc ttctggattt tgttgagatg ctgcttgttg ttctttctga
     2581 tcttgactga taatattgcc agcatcatct gtagtcaatc cattttcata aagggccacg
     2641 ccgaaagctt cccactcttt gttggaccaa tttgcacgat cagctggagt tgactgtaaa
     2701 gtgcgttgtt tcatctgttc atatgtttct tcttgaggtg cggtttggat tgtactctga
     2761 ctggagctta taactgttgg gctaggttcc gccgtaactt ggttggagct tgtaactgtt
     2821 gaactagaat ctgttttaga tgtagatttg ctagtagatg aactggtttc agttgtttct
     2881 tttgttttac ttatttttgt ttcttggtta gaagtggcat ctgttgattc agctttttta
     2941 ttatttgaac aagctgaaag tagcagagca gtacttaaca acaacataac gctaactttt
     3001 ttcattttat actcctcttt tctttgatac aataagttgt aaagaagcct actataggtt
     3061 taacatccca cgtccacaaa ctttggtcgg tgggcgtgtt tttttgttta tagactttcg
     3121 aaaactaaag tagcttggat tctatcgcca ccaccgaaac ctttactacc accgtttgta
     3181 gtggaaatag tgtgtaatcg ataaccttta gaacactgtc tatttatagt gttttcaagt
     3241 tctgataggt tttgcgaccc ttttcctata aacttttctt ttaatacaac ttgcaataca
     3301 acgtaacttg gcatattctt ttccttcttt ctactatgat aataaatact aacccctagc
     3361 cggagcgcca actacgactg gggtattttt tagaatacga ctttcccgat tattctaatc
     3421 tgttccggtg agacgactct atcttcatat aattcgttta tagagcgtag aataatcgtt
     3481 tgactctcat aatccttata tacttcttta cacgtcacac cgtcaccatc tatttcaacg
     3541 atagcaatct caccgtcttc cacgtctggc tgttccttaa caaatacaaa ttgtccattc
     3601 ctgataactg gttccattga atcgcctata ataggaacag caaaatcagc attgtttgga
     3661 actctttcaa attcttcttc ctctacaact gtatcgccat aaactaattc agtcgggttg
     3721 gcggcagaag ctgaattttt gattgtcgga agtttttcct ttttaggaaa gtgcaccacg
     3781 tttcctcgat tttgctcttc gagttgctgt tcagcaaagt tgtagacttt tgtttgtctt
     3841 ggtttttcta attgattgta gattgtttca atggatggaa tgttattttt cttatcttct
     3901 ttaggaacat cgtaaccaag caaccatgcc tcactcacat ttaaagtttt agccaacaaa
     3961 taaagcttgt gttggtcggg cgatgactta ccattgacat attgcgatag atgacttttt
     4021 gacattttta ttccagtttc tttttgcaaa ggtaaagaca tattcaaaat atcaacttgc
     4081 tttaaatttc tttcagacat taactgatta agtcgttgct gagtcgtttg cttcatctta
     4141 tacctccttt ctgatactta ttatatacct tattgaacat aagttcaata aaaaagttaa
     4201 attaatttga acttttatgt tgacaatgtt ttatgcagga gttatacttt ggttacaaag
     4261 ttcaaaacat ttgaactaga aaggagcgaa ctaatatgag ttttgattat tctaagttgt
     4321 cgggaagaat tgttgagaaa tatggaacgc aatacaattt ttcaatcgct ttaggtttgt
     4381 cggaaagaag tttgtcatta aagttgaata accgagtacc atggaaaagc accgaacttc
     4441 aaaaagccat tgaattgcta gatattccaa aagaagaaat tggcgaatat ttttttaatt
     4501 tagaagttca aaatgtctga acttaagaaa ggaattcgaa aaaatgaaca cacaaaaatt
     4561 tgaaaacaat ctatttgaat tagaagttaa gacggaaaat ggtgaatcgt tatttaatgc
     4621 tgaaacagta gcaagaagtt tgggtttcgt ggaaacaaaa aacggtaaag aatatgttcg
     4681 gtggagaact gtcaatggat atttaaaaaa atacctttcg caagaagttg cgaaaaacga
     4741 ctttatcagt gagccaatgg tttacaaatt agcctttaaa gcaaacaacg tacttgctga
     4801 aaaattccaa gactggttag caacggaagt cctacctcaa atccgaaaac acggcatgta
     4861 cgcaacagat gaactactta acaatccaga cttgctgatt gaagtagcaa ccaaactcaa
     4921 agaagaacgc acgttacgat tgatagctga acaacgagta aatgaattac aaccgaaggc
     4981 agactattac gaccgaatct tgaacaacaa aggattagtt acagttagta cgattgctaa
     5041 gaactacggt atgagcgctg tatcgtttaa taaattgcta catgaattag gtatccagtt
     5101 caatcaaagt ggcacatggc tgctatacag taagttccaa gacaaaggat acacgcatat
     5161 tgagccattt gattatgaag ataaaaacgg caatcggcaa gtaaaaacaa ggatgaaatg
     5221 gacgcaaaaa ggacacatct tcttatatga aacattgaag aagaataatt acttaccgat
     5281 gattgaacgt gaacaaacag cataggaggt aacactatga acgaggaaat gagccaaaca
     5341 atcaaggtac aaaaaatgat agatgatcta acacatgcaa ttgatagcca agccgataaa
     5401 atcatcaagg aattgcaagg acaaaaagtt tgggatgcaa aaatgctttt caagactatc
     5461 aaatttgaaa tgaatccaac aaaaagaaag ctcgccgatg tgttggaaga aaagttggct
     5521 tccgctatca acgagcaaga attactattt gaaaccgata cttttaacag ctgatgtttt
     5581 atagatgaca gtttcatccg aatctatatc attatagatt cgattattgc aatgtacgaa
     5641 ttataaatac aggaggaaat aaccaatgga gcaactagct ttagttaacc tctcagacct
     5701 taaagcttct ttggcagaat ctgagattgc aaatgaagtg tgggatacaa agcaagctgc
     5761 ggaatattta aaaaccacaa cacgcacgct gactaaagat gccgaatctg gaaagattcc
     5821 agcagcgaaa gttggcagag agtggaggtt tagtagtatc gctttgtatc agtatctaaa
     5881 aggaggaaaa acatgggtaa attcaacaga gcactagtgt tcagcgcacc cctaatcgtc
     5941 tatgctttag gactttgggg aagcagacaa gcattaatcg gcacgatcgt ttacatggtt
     6001 tggattttta tggggcttga tgaagctgag tacagagcga aaaagcccac cgagggggct
     6061 gactaaagtg tggttctgtt tattaggtgt ttatctcgtg gccgttttag taaataacta
     6121 catgagaaaa cgtggcgaat actggtatac atcctatgcg gttctagtat gtctaatgct
     6181 tacaattttt ctaatgattt attcaaaata ggtaatactt tgtcaatcaa ataagtttca
     6241 agagattttt gttctgcatc agtgttgccg taaccaaaac ggcttaagaa aattttcata
     6301 acctctatat cttcattttg aatatatggc aggactgcat aacctgatgc ttttacagca
     6361 gatatatttt tatcagtgtt actaccaata caaataccca cattgttgag aaatgttgag
     6421 aaagttgttt gtattgtttg ggtttttgaa tcgtgttttt gtaattcaat ttctttatcc
     6481 ttcatcgatt caacatgctt attatttata accgctgtaa tcgaagggga tataagagct
     6541 actaaagcta ggataatcga aattgtgatc gtgttatcga aactcatttt ttcaccatcc
     6601 agtttttaaa ccattatatc aaaaaggaga gaagaaataa tgcaagaatt agtaattttg
     6661 aaaaataaaa aaagacacga acgccagcaa gcatgatcgt gtccaaaaga aaaatctttt
     6721 aaaccattat aacacaggag gaaataaatg atctcagtta aagggttagg cgatgaaata
     6781 ttcgaagcaa tgatgagcaa agcacaacag gacatcaaag aaaaaatcat ttctgctgca
     6841 gcgcaaggac aaactagctg cactgttcga tcgagaggaa cgacaccaag ttttcttttg
     6901 gcgctagaag atgaaggcat tttgaatatc aaacgaagag atggcagcgt cagattgttt
     6961 tggcaatttt aggaggccgt gatgaaagat tttgattctt taggcgccag acagcaacca
     7021 ccaaatgaag ctagtcctgt tggagttgat tggcaagaaa atccgttata tccaggtgat
     7081 acttgctatc taacagaaga aggctatgta ccagtagacg caattctaga gtacgtccag
     7141 caacattatc caaagattga attaggagga atttaggaat ggcaaatgat ttaacacaaa
     7201 caacacaacg ttcattagat gagcaggtaa tcagtaatct tggacggtta caagaacaag
     7261 gtttagaaat gccaccagga tatagtccac aaaatgcgtt aaaaagtgct tttttcgaac
     7321 tcactaacaa tacaggagga aacttgttgc agatggctgc aaacaatcaa gaaatgaaaa
     7381 cctccatttc taatgcactt ttagacatgg ttattcaagg gttatctcca gcaaaaaaac
     7441 aatgctattt cattaaatac ggaaacaagg ttcagctaat gcgttcatat tttggaacaa
     7501 tggcggtcct tgatcgtgta actggagggg cagatattac gcctgtagtc gttcgacaag
     7561 gtgatgagtt tgaagttgca atggatggac caaacatggt tgtcaaaaag catgaaacaa
     7621 aattcgagaa tttagacaat gaaatcattg cagcctatgt agtcatcaag ttaacaaatg
     7681 gtaaagagac cacaacagtc atgacgaaaa agcaaatcga tcaaagttgg gctaagtcaa
     7741 aaatgaaaga ttctggaccg caaaaggaat ttccagaaga aatggctaaa cggacagtaa
     7801 tcaatcgggc agctaaaaca ttgatcaaca caagcaacga caacgattta ttagttcaag
     7861 ctgcaaaaga tacgttagaa aatgaatttg acaatgatcg gaaagatata acgccacaaa
     7921 cagaaaaagt ggctactctc gaacaaaaat tcttttcaaa caaaaagatt actgaaccaa
     7981 tacaaaaaga accagatccg attgtgattc cagatgatat ccaaaatgaa gtaactcgag
     8041 ttgctgatgt acctgcccat ccagaaatag agcaagcaca tttgatcgaa aatgaggata
     8101 tcaatccaat tcaagaagaa ttgatagata ttccagactt tggacgcgag gaaggtgtag
     8161 aagatgtctc agaatttgaa gacgacgagt accctttctg atgagaatta ttactccaat
     8221 gaagctgact ggcagtacat gtcaacatca caatataagt cctttttaaa atgtgaagcc
     8281 gcagccttag caaagttgaa aggcgattgg gcaccgacgt cggatcccaa agccttactc
     8341 gtaggaaact acgtacattc ttattttgaa tcaaaagaag tacatgaagc gttcaaagaa
     8401 gaaaataaat ccaaaatgtt ttctagtagg aagccgtatg gattgttgaa agatttccaa
     8461 attgctgaac aaatgattga acgacttaag caagaagaag cattcatgaa catttatcaa
     8521 ggtgagaaag aaacgatcgt gactggggaa ctatttggaa cgacatggaa aggcaagatt
     8581 gattgcttga atgttgagga tgagtatttt gttgatatca aaacaaccaa agacatgcat
     8641 gagcggaaat ggaacgaaaa ctacggatca agagaaacgt tcattgtcaa ttttggttac
     8701 gtacttcaaa tggcaatcta tcaagagctt cttttacagc aatacggaaa gatgttcatg
     8761 ccaatcatcg ctgcggtatc gaagcaaacg cctagtgagg cgaagttgat aacgattgat
     8821 caagataaga tgagctacga gctaatcatg ctaaaagaaa atattgaaag aattgtcaaa
     8881 gtgaaaaatg gcgaagaaag gccgatacct tgcggtacgt gcgagtattg tcgaggtaac
     8941 ctttcaatta ctggattcac tagcatggac gatctttaaa aggaggtgct ttgcgaatga
     9001 atctaggata catcaagtta taccgtaaag tgactagttc attcgtttgg accaattccg
     9061 atatgttcaa gctatggata ttgtgtttaa tgaaggctag tcatgaagat agaaaatttt
     9121 tatttaacgg tcaagaagta cgtttgacaa gcggacaatt cgtcacagga gcccatgcaa
     9181 tagcaaaaga gtacaacgaa ggagtcccga gtgacaaagc aattgcatgg cgaacgctat
     9241 ggagatggct taagaaattt gaaaatgaag aattattgac aatccaatcg aacgctagat
     9301 atagcgttat aacaataata aattggtctg actatcaatc gagtgacaag ccagtgacaa
     9361 gcgacggaca atcgagtgac aagccagtga caacaaacaa gaatgataag aatgataaga
     9421 atgataagaa taataatcct cgcaactctc gaaaaacacg agagtatgca gatgacgatc
     9481 caaataaaaa attggccatt cttttattaa aactcattcg aaagaatcgt agtctcaaag
     9541 aacccgattt gaacaaatgg gccaatacga ttcgtttgac gattgaagct gacaaacgaa
     9601 ctggaaaaga agttcaagac atgattgttt gggctactaa ccatgatttt tggtccacag
     9661 tcatttcatc gccaactagc ttaagaaaaa atttcgatac aatggtgggt caaaaaaata
     9721 aaagaaagca acaaaatatt tctaatgatg agttaccaga aacaggtgag gattggtaat
     9781 gaataaaaaa ctaagtgcaa tggctgcacc ttacggcgga ttaaaaacag cagatcataa
     9841 ttgcccgaaa tgtggtgatc cattgtatat ctggaaaaca aaaaataaag atggtactga
     9901 tcgatgcggt cctacatgta tcaataagat ttgtggttat cgagaaatgg taactaaaaa
     9961 tcaaaaagaa gctatccaaa aagcgaatga agcaatgaaa aaggacgcta tcaatcgaat
    10021 gcttaacagt tcaatgatta cagatgatgc catatggacc ttcgattttg atggatacaa
    10081 agtagttgat caggaaacag cacaaataaa agcgatggct caagaatggg ctaaaaaaat
    10141 tgtaaatggt agcacgattc atgcagtgat aaccggtaga acaggagccg gcaaaaccca
    10201 tctaggcgct gcagtgatta aagaagtcat gagggcatct aattataaaa ttgcttgttc
    10261 atttatcagt tatcgagaac ttttagaaca gttgaaattt gcaatgaatg atccagaagc
    10321 aagaaaagcc gtaacaggtt cgctgatggc tgagataaag aaatcagatt tcgtggtaat
    10381 cgacgatttg ggtgcagaac taggaagaat ggaagaaaac aaccaggcaa cgccttatga
    10441 tgttgatgtt ctcacatcgc tcacagaagc tcgtctaaac aaagctacga tattcacaac
    10501 caatttatca tcgaagcaat taaaacacgc gtacggtgag cgagtgttct ctcgtgtaat
    10561 gaatggaacg aaagggaaca tagctgtatt caaaacaacg acagacaaaa ggaggaatcc
    10621 agtttgacct ttgtagtgaa aaaaatgtgc tacttagata gccgagggcg aggagaagct
    10681 agtgtcgagt ttgctaaaca ccacacgact aaagaagagg ctgaccttgt tgcaagtgtt
    10741 tgtggcggcg aagtagtcga agttattaaa cccaaaagac gattttcaaa acctaagaca
    10801 aagccagtga aaaaagagtg ttgttgtccg aaaagtaacc aagcatggat gagaggtgca
    10861 aaatgacttg tttaaaatgc aacgatgaaa cagttatttg gtataagaca ttgcttggat
    10921 ggtcaacttg tgaaccttgt ccaatatgca atgaaaatgg acgacgttcg aaagaacgac
    10981 tcgaaagact aaagaaggag tatagcaaat ggcaacaaga agcaaatacg gaaacaaaaa
    11041 gcacgaagta gacggcatca cttttgattc tatcacggaa gcagagtatt acgatctagc
    11101 cttgtggcaa gcacaggcga atggttggaa agtgaagctc caggaaaggt ttgagctgat
    11161 gccgaagttt gaactagagg gcaagaagta tcgcaagatc gagtatattc ccgacttcac
    11221 attttacaaa agcggcaagc tggtcaaagt cgtagatgtt aaggggatgc agacaaaaga
    11281 ctttaagatc aaggcaaagt tgttctgtca tcaatatcaa gtgccgttga tattagccaa
    11341 aaaatatcgg aatacgttca aggaagagcg tttttaacga ggtggtccat catgacaaca
    11401 gaagaagtga ttcaaatgcg aattcgaagc cttcggcgtg agattgacga tctggaacga
    11461 acaaaggcag tgatggtcaa tgaaacggct agaaaggcaa tcgatttgca catagagaat
    11521 ttaagaaggg aaatccgtag attggaggaa tgagcgtgga taaggaagcg gcttggcgaa
    11581 aattaatgtt gctgattcaa gatgagaact ggcaagaaga tgaagcagtg gttgctgaag
    11641 ttcagcgtct aggaaagatt gctaacggac gtatacgaaa aaagccagac aaaagaaaac
    11701 agcgcaaagg gaaaatcgtc gttgttttac acgaaggcaa aattttgatg caaggaacag
    11761 ctagtgagct gtctgcagaa actggatata cgcgtgggac tattcgaacg tacgcttggc
    11821 gaaatcacac cgatcgaaaa gggcacgaat ataagtattt ggaggaagaa aaatgaacga
    11881 aaacaaatta atcaaattgg gtgtagcagg agcagtaatc gtaggtattg gagttatcgg
    11941 aggatttaag ttcttcgaaa aaatcgataa tggatatgtg ggtgtgcgct attcaatgaa
    12001 cggcggtatc aaagatgaag cactgacgca aggtgtgaaa tttgtaggga ttgacaaagt
    12061 gatccaatat ccaattcgct tgcaaactat ccaatcaaaa aatatttcag tatctacaag
    12121 cgacggcaaa aagacaacga ttgatatcaa atatgactac aaagttgatt caactaaagc
    12181 agcaaaaatg tacaaggaat ttgggaatat cacttcggaa gatatcgaaa gtggatggtt
    12241 aaaatctaag cttcaaaagg tcgctcgtga agtttatgcg aaatatagtc tgcttgatgt
    12301 cctttcagga gattcctcta aagttgaagc tgaggtatta acgaactttg ctaaatcagt
    12361 tgaatctaaa gggtttgaag tcgaagacgt aacacttggt gttccagatg tcgataaaga
    12421 aacacaaaaa tcaatcgatg cgatcattcg agctggtcaa gaaaatgaaa aagcgaagct
    12481 agacgcagaa actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa caaaagctgc
    12541 agaagcagag gcagaatcta atcgcaaagt cgccgaatca gtaacagaca atttgattcg
    12601 ttatgaagaa gctcaagctc gcaaaaagca tggatgggta acagtaaatg gagcagatac
    12661 tgtagttacg gatgaagcag gcaaataata tgggattctt tatggctaaa attctcttgt
    12721 tcttaggttt agtaggagca gcatatctcg tgtatgcgct cctttcccaa actgatgaca
    12781 aagaagatga caacaacgat gaaatgaaat tttaggagga gaaataatgg acgaactaat
    12841 cacaaaagta gagcagtggg ctaaagacaa gggactggat caagcagatc caaaagcaca
    12901 gtttttgaaa gtagctgagg aattcgggga aatagcttcg gcgatggcaa gaagtaatga
    12961 tgaactattt aaagatagcg taggagacgt tatcgtcacg ctgattatcc tttccatgca
    13021 aaaagggaca aacgtacaag agtgtttaga aatggcatac aacgaaatca aaggacgcac
    13081 agggaaaatg gtagatggtg tattcgtgaa gtcgagtgat ttggagggca gcaaatgata
    13141 aaaaagctca ttcaattcag catggattta tatgatatcg aatcaggagc aacactatct
    13201 gtggaatcgg accatctaat cataaatttt ggtggaaagc gccagattat tttgtgggta
    13261 gttgatgatg tactgtttcc agaaattgtt catgatttcg aagaatcaaa agcggttgag
    13321 tttgaaatag tgaaaaaagt aatggaattg attgaaaaat acgaggagga cagcgaatga
    13381 taccgaagtt tagagcgtgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc
    13441 attttactaa aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga
    13501 aatcatatca cgtgcgaaac atcgacctca tgcaatccac aggaatgaaa gataagaatg
    13561 gtgtggatat atttgaaggg gatatagtat tagtcagcgt gcgaaatggc ttcgattact
    13621 tagataataa agtttgtatt gtcaaaaatt caatagatta ttccggatta gtttgtgcca
    13681 ctgttgatga agacttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt
    13741 atgaagtcat cggaaatata tacgagaata gcgagttatt ggaggtagag tgattatctt
    13801 agctactgac tacatcgatt ctttaaaaga tgaaaatggg aatgtcccag cattgctaaa
    13861 ttgtggaatt aaaagcaaga atagaatcat tttgttacgg atatcggctg atttagcaaa
    13921 atatctttat ctgagcaaga cagcagtata ttacactgcg gctacacgtt atcagtacaa
    13981 gggaaaaaca atcagtcagg attattatga tcgtttaatc agtcctgata tgtatgggaa
    14041 ttcagaatca gcaattaagc tgtttggcgc aatagagata tatgccgatg attttccaaa
    14101 tttatggttg aaggaggaag cggaatgaaa tacgaaatac cactaagtga agcagacgtc
    14161 cagtcaatta ttaacggtcg ggaggttaat aaaaaacttc ctgatggtac tgaattagtc
    14221 atcagacaaa gttatttgaa agatatggca gctccagtat taattgatcg ttttaacgtg
    14281 actgattctg tggtagagaa ccacttgaaa gaatttcgat caagtataaa cgacactttc
    14341 agattaggga gttgattgac aatgaacacc agacaccgca gagtagcaaa actaagaaaa
    14401 caggaactga atgtactaaa gacaaagttt gaaaaagaat atggaatttc agcagaagaa
    14461 acatataaag tggcaagtca gtgtgttgct gatgcaagtg atgctattcg taagtttgga
    14521 atttcgatat taagtgatga tcgtaaatgg gaggaaaaag aatgaaacta aaagacggat
    14581 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaatccta
    14641 aaacacgtaa gaaaccaaaa gtcaaagtcg gtgacatggt ccgctgtgaa gcagaggagt
    14701 tcatttatcc atttcgtgga tatgtagaac acgtctataa tcactcagcg atcattcgca
    14761 ttgaaaacac gatggaatgt gataaatgga cagctaaaag caaagagaat ttagcagtag
    14821 tgagattggt ggatatggaa ctaatcaatg acaaataaaa aagccggatc gctccgacta
    14881 acataataaa acagacaagt ttattatatc acataaaagg agcagtttga attgatgcaa
    14941 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
    15001 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattatt
    15061 acggatatgc cgaaggcacc gaagcacggc aataaggcag aggatgcatt gattcagatg
    15121 atggatatag aagcggagag agacgcgatt ttagcggctt tgatggctct tagtctgatt
    15181 agtcgtcaga tactctacta cagtttttgt gtgccagaca gcttctcaaa ctacagaatt
    15241 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaaatcgga agctctaata
    15301 gagtttgcag aagcatataa acacggaaga ataattgctt ataaataggg ggaccatata
    15361 atgtggaaag attatgtatc gttgaaagag ttgaaaaaag atcttgtttt caaaagaatt
    15421 gtagaatggt cagagagtga attaatttta gaagatggaa ccaaaatgga agttgtatgt
    15481 agcgaatcag attgctgcgc atgggccgaa ggtgaattca aaaatgtaaa gcttgatgca
    15541 gtgattacag atataaagat ttttgataaa ggtaaccgtc tctataatgg tgacggacat
    15601 acctcttatg ctgaagtcgt tgtttatcat aatcgtaatg agatcgctaa agcagaatgc
    15661 acagcgaatg atgggaatgg cggctactat tatagtgttt gtgctttaaa ggtcaaagac
    15721 aaactttgta tagtgactga cgcataaaaa aatggcggtt ttttggcgga atgatggcgg
    15781 tttttagcca tttatcagtg atattatggt agtgtcgaaa gattagtgat aggtctgaga
    15841 caaaataata ataaaaggaa catcgtttta ttattgtttc acaattaagc ttcgatagac
    15901 agcaacggaa atattaagaa taaggatgtg aattttaact ccttctaaat tgttcttatt
    15961 atctatcatc cgttgctgtt tattgttaca tacagatcgc ttaggcggtc tttttatttt
    16021 ggccgaaaac ctacattttc gatggccgat tattggagga ggaatagcat gttcaaacta
    16081 tctgaaatca ttaagaagtc cgacgttgag aaattagaga tgttaaaaaa gaagttgaag
    16141 aaatagcagt ctcttcgtga ggctgtttta ttttgctcac aaaaatagac cactatcggg
    16201 taatagtggt caggaattaa atgaaaaaga tgttagggtt gttagctaag tataacatca
    16261 taacgcttac aaaacaatgc aagaaggata aattattatg agaacttact ggtatgtgtc
    16321 attaaacaat aaatatccga agccaatgaa aggacagcat aggcgtgtag tcatgtctgt
    16381 tcaaatgaag gcgaagtatt cgattgtaga aatgctaaga gaagctacac caaaagagat
    16441 tgatcagtat aagttggttc ttattggcat tggttggttt aaggatgaac atattcaaac
    16501 aaacatgaag aggtggttga gatgaagtta tattatgttg aaaaagcaaa ttgttgggtg
    16561 gttgaaggct gtctgtcaaa gtacgctaca gatattatat ctactcaatt ttcagagttg
    16621 ggattgaagg ttatcttttg tggtgagttt gtcagtgtga aagaagcggt attgccaatg
    16681 acagcaaaga gcagacgctc gcgtggttca agtt
//
LOCUS       DAZAFF010000059        16680 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000059 DAZAFF010000000
VERSION     DAZAFF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.059"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..792
                     /locus_tag="ACG9BB_001861"
     CDS             <1..792
                     /locus_tag="ACG9BB_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFN2837793.1"
                     /translation="ASTQQLQSRKSKLLFLALVFEDQSYVIIDQSGHPVEYSPAKYTY
                     QEGLSCKKWKLINEEPIELSRWINRKEDIPVLIEEKRSGKELANCWVGLPEERFLRYK
                     KWATPSGYLCGTYAAAVLLAYYQDYRKGWMLPNEIRKKNTADSLVLTKALRSQIQPLG
                     LPTIPFQVSTGISSFLKKNGNHERARATLLGSWQRATKRIREGKPVMIGILKVLGSTY
                     GNHWVTAYAYFETETGERYYKVHDNWGDYHKVIPASWSNGTVSLP"
     gene            872..1846
                     /locus_tag="ACG9BB_001862"
     CDS             872..1846
                     /locus_tag="ACG9BB_001862"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFN2837794.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            2026..3171
                     /locus_tag="ACG9BB_001863"
     CDS             2026..3171
                     /locus_tag="ACG9BB_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFN2837795.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            3308..3652
                     /locus_tag="ACG9BB_001864"
     CDS             3308..3652
                     /locus_tag="ACG9BB_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFN2837796.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            3923..5209
                     /gene="efmA"
                     /locus_tag="ACG9BB_001865"
     CDS             3923..5209
                     /gene="efmA"
                     /locus_tag="ACG9BB_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFN2837797.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(5347..5946)
                     /locus_tag="ACG9BB_001866"
     CDS             complement(5347..5946)
                     /locus_tag="ACG9BB_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN2837798.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            6350..7471
                     /gene="mnmA"
                     /locus_tag="ACG9BB_001867"
     CDS             6350..7471
                     /gene="mnmA"
                     /locus_tag="ACG9BB_001867"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFN2837799.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            7717..9138
                     /gene="arcD"
                     /locus_tag="ACG9BB_001868"
     CDS             7717..9138
                     /gene="arcD"
                     /locus_tag="ACG9BB_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFN2837800.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            9152..10270
                     /locus_tag="ACG9BB_001869"
     CDS             9152..10270
                     /locus_tag="ACG9BB_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFN2837801.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(10330..11622)
                     /locus_tag="ACG9BB_001870"
     CDS             complement(10330..11622)
                     /locus_tag="ACG9BB_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFN2837802.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            11772..12332
                     /locus_tag="ACG9BB_001871"
     CDS             11772..12332
                     /locus_tag="ACG9BB_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2837803.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            12461..12838
                     /locus_tag="ACG9BB_001872"
     CDS             12461..12838
                     /locus_tag="ACG9BB_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFN2837804.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            12771..14843
                     /locus_tag="ACG9BB_001873"
     CDS             12771..14843
                     /locus_tag="ACG9BB_001873"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN2837805.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(15054..16001)
                     /locus_tag="ACG9BB_001874"
     CDS             complement(15054..16001)
                     /locus_tag="ACG9BB_001874"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN2837806.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            16247..>16680
                     /locus_tag="ACG9BB_001875"
     CDS             16247..>16680
                     /locus_tag="ACG9BB_001875"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFN2837807.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         5429 a         3007 c         3579 g         4665 t
ORIGIN      
        1 gccagcactc agcagctgca gtcaagaaaa agtaaattgc tttttttagc tctggttttt
       61 gaagatcaaa gttatgtgat tatcgatcaa tcaggacatc cggtagaata ttctccagca
      121 aaatatactt atcaagaggg tctttcttgt aaaaaatgga agctgatcaa tgaagagcca
      181 atcgagttat ctagatggat caacagaaaa gaagatattc ccgttttgat cgaagaaaaa
      241 aggtcaggaa aggaactcgc aaattgctgg gtgggattgc cagaagaacg ttttttgagg
      301 tataagaaat gggctacccc ctcaggctat ctttgtggta cttacgcggc agcagttctc
      361 cttgcctatt atcaagacta tcgtaagggg tggatgcttc caaatgagat tcgcaagaaa
      421 aatacggctg attcgttggt tttgacgaag gcattgcgta gccagatcca accactagga
      481 cttcctacga ttccttttca ggttagtaca gggatttcta gttttttaaa gaagaatggc
      541 aatcatgagc gtgcacgagc aactcttcta ggttcatggc agcgagctac aaaacgtata
      601 cgtgaaggca aacctgtgat gattggtatt ttgaaagtac ttggcagtac atatggcaat
      661 cattgggtga cagcgtacgc ttattttgaa actgaaacag gagaacgtta ttacaaagta
      721 cacgacaact ggggcgatta tcataaagtc attcctgcca gttggagtaa cggaacagtt
      781 tcacttcctt aagaatttgc tgaaattaac agaaaatctt ggaaaaatat gttaaactat
      841 cgaagaatag agaagttttg gggaggacta catgagtaat aagtatcagg atgacacgtt
      901 gaagattttt agtttgaatg gcaaccgtcc attagctgaa aaaatcgcga aagtgttcgg
      961 aacagaatta ggtaaaagcg tcgtgaagca atttagtgat ggcgagattt ccatcaatat
     1021 cgaagaaagt attcgagggg atcatgtgta tatcgttcaa tcaacgaatg cacctgtcaa
     1081 tgattactat atggaactac tgatcatgat cgatgcaatg aagcgagcaa gtgcgaaaac
     1141 gatcaatgtc gtattgcctt actatgggta tgctaggcaa gaccgcacgg cgaaacctca
     1201 tgaaccgatc actgcgaaat tgatcgctaa cttgatcgaa gaagcagggg caactcgagt
     1261 actgacttta gatctgcaca cggttcaagt acaaggattc tttgatattc ctgtagataa
     1321 tttatttacg atgccgttgt ttgctcatta ttaccgtcaa ttaggtctga cgggagatga
     1381 catcgtagtt gtttcgccaa aaaacagcgg tgttcagcgt gcacgtagtc tttcagaata
     1441 tttgaacagc acattagcca tcgtagatca tgcagatgat gcttcaactg agggtggtta
     1501 tgtgatcggt gatgttcaag gaaagacatg tatcatggta gatgatatct tgaacacagg
     1561 agcaacgctt gcacgagcag caaatgtatt aaaagaaaac ggcgcaaaag aagtatatgc
     1621 ctgtgcgtca catggattat tgtccgatca tgcgaaaagt attttagaca atgcaccaat
     1681 caaagatatt tgtatcactg attctgtttt cacagaagaa aatcgtcatc cagaaaactt
     1741 aacaatcgtt acatgttcag aattaatggg tgaagcggtc aaacgcatcc atgaaaatac
     1801 accgatgagt ccgttattcc gtctggaaga aaaagaattc gaataaacaa aaaaaagagg
     1861 gaaagaagtt gtgacagaag tgagcagctt caaggagctg cgacaagact tttgtcacag
     1921 ctcctttttt ctatgcccat gaaacgtcct ctagacaaaa atgagctctt ctttggtttg
     1981 ttcgctattt tatgctagaa tgtgcctagc aataatttgg aagggatgaa tgaagtgaaa
     2041 actatttatc tggatcatgc ggcaactacc cccatgcatc cacaagtaat tgaagcgatg
     2101 acggcaatca tgcaagacac gtttggaaat ccttcaagca tacatggatt tggtcgccac
     2161 gcgcatgaaa aattggaaac agcaagacaa atcattgcag acagtctgca agcaaaacct
     2221 cacgagatca tctttaacag tgggggaaca gaaggggata atacagctat cttagaaaca
     2281 gcgttctcgc gtcaaaaaga aggcagacat ctaatcacta cagctatcga acatcctgct
     2341 gttcttaata ccatgaaata tttagagaca aaggggtttg acgttacata tctgcctgta
     2401 gatgaaaaag ggaatctgtc gatcgaagag gtcaaaaaag cgttacgaga agatacgatc
     2461 ttggtctctg tgatgtacgg gaacaatgaa atcggaaatt tgatgccaat caaagaaatc
     2521 ggtgaacttt tagcggatca tccagcttat ttccatacag atgcggtaca agcgtacgga
     2581 aaccaaacga tccatccaga agaattagga atcgatttat taagtatctc tgctcataaa
     2641 atcaatgggc caaaaggcgt cggtttctta tataaacgag acggagtaaa tatccctgca
     2701 ttgcttttag gcggggagca ggaagaaaag cggcgtgcag gtacagaaaa tttagcaggg
     2761 atctggggta tggcaaaagc agttgaactg ctgtcacctg aagaacgtga agcacgtaac
     2821 aacaaatata aaggattcca gcaattgatt cttgatacat tagaagcaaa cgaaatccct
     2881 tatcaagtaa atggtgattt aacgaataaa ctccctcatg tgttgaatct tcgcttaatt
     2941 ggcgaagcaa atgatttgct actgatgaag cttgatctta atggggtcgc tatttctact
     3001 ggttcagctt gtacagcagg aaatatcgag ccttcacacg tattagaagc aatgtatgga
     3061 gaagatacac cgatactaaa agaatccatc cgtatcagct ttggtttggg aaataccgaa
     3121 gacgacatcc agtatttcct tgatcaatta gttcgtgctg tcaaaaaata actagtgaag
     3181 tcattcttat atgaacagat gtctgagaga acgccttcat tttttaggga acttaactga
     3241 aaaacctagt aaaaatcagg ataaactttt ataataaaga gaaactaatt ttatcgaggt
     3301 gaaaatcatg gcatttgaaa caacagcaac ggtactgggc gcaccaatta gctatcgttt
     3361 gcatcctgat gcaaaaagat atacattgcg cgataatgga tttacagaaa caaacggagg
     3421 caattatcaa ttgatccgac cattagatgc cacgccgcaa agcaaagaag gattcaaatt
     3481 aaaaatcact gtatcaaaag atattcaaac attgaaaatg tcgatcacta cagcaaatgg
     3541 attgaaagca gtcaatattt tcaaagatcc aaaacagaaa atgagccaag ataaatttta
     3601 ttttctgatg gatgggatga ttagccgtgg gttattcgaa aaagtcgaat gatctgtgga
     3661 tataaaaaga caactagttt tgaaacggcc tattgttaga tgactaactt tgtaaggacg
     3721 acgcaaatta gttgtctttt ttatttttaa atttaacaca tgttgttatt tttaatattt
     3781 tgctgattta ttgtttctaa gtaaagaaat ttaaattgaa acgattgtta tttagggaaa
     3841 aaggtatttt ttgaaaaaag aatcccgtat aatgattgtt gtgcaaacgc tatacaaaat
     3901 gaaaaagaaa agagggatgt ttatggaaaa tgaacagtcg gtcgttttaa cgaattggaa
     3961 gagaaattat ttgttctttt tatcgggaca gtttttgtca ggaatcacta gtatggtcgt
     4021 ccaatatgcg atcatctggt atttgacaag agaaaccggt tcggcgacaa ttttgagttt
     4081 tgctacttta ctaggaatga ttcccatggt tttactaagc ccatttgtag gaccgctagt
     4141 ggatcgctgg gataaaaaag ctcttttgat tgtcacggat attattgtag cgattttcgc
     4201 gcttatttta gcagtagtcg gaacgatttc agaatccttc ccgatttggc tagtttttgt
     4261 gtctttgttt atgcgttcgg tcgcacaaac attccaaatg ccaacgatcc agtcgattat
     4321 gccgacaatc gttccatctt ctcacataac aaggacgaat ggacagttag gaatggtcca
     4381 atcagcgaat ttcatcattg cgccagctct aggtgcggct ttgttttcag tcgttcctgt
     4441 caattacctt attttattag atgtattggg ggcagttttt ggtgtcggct tattgatttt
     4501 tgtgaagatc ccaaaagttt ctcctgaaat attggaagtt ccgcttacta tttttaaaga
     4561 tgcaaaattt gggctgcagc aattgatgga taataaaggg ttatggtaca taacgatcaa
     4621 tggtgcattt gtgatgctgt tgttcatgcc agcaatcagt ctgtatccat tgatgacgct
     4681 agattacttt ggtggttccg ttggacaggc gggagcagtg gaagtagtct acgcagtcgg
     4741 aatgctctta ggcggtgcac tgatcagttt tataggaaca tggaaagacc gaatgaaacc
     4801 tattattata gcctacatca ttatgggcct gacgatcggt gcaagcggat tggttccaaa
     4861 cgatagtcaa gggttcttgt actttcttat cttgaatgca ggagcaggtt gtgcaacgcc
     4921 atatttcaat acactgctga tggcaatgat ccagcagagt tatgaatcga atgttttagg
     4981 tcgtgtctta gggaacttca actctttgat gaacctggca ggtccaattg gattattatt
     5041 tgcaggacca ttagctgatc gtttaggggt agaaaaaatg ttcttgtttt ccggaatcgg
     5101 gattttatta tgcggaatcg ttttattttt aacgtcagct gcccgaaatt atgataaaga
     5161 attacagaaa aaactggtaa aagaacacca tgagcaaaaa gacgaatagt atggaatgga
     5221 gttgtgacag aagtcttgtt acaactccat ttctgatcct aatgttcctg tggcaataag
     5281 cctactcaaa tgaaaaatat gagtaattat ctttatttgt ttaatacttg atatagggca
     5341 gttttttcac gacctatcgt acttcctctt tgcttcatag atttctttga tttgttgctc
     5401 agataacgga gtgccaaatg gtagtttttc ctttaagagt tctctttccg gtgcgtctac
     5461 gcctacattg acatcgttct cgataggtac gctgtaatga tggatatgtc catgaagatt
     5521 gcggatagtg tcgttaggcc cgagcaacat cggataatga gtcaagtaat attgttgatg
     5581 gtcgaattta acgatggcac ctacatcata aaaataaaat ttgggacttc cgtcagtaag
     5641 cctaggatca tttttttcta aataacggaa aaaagcgcga ctgtcatggt tccctttgat
     5701 caagtggatt gttccttgca gttgagtgac cagttccaaa atttctgcat ttgctttttc
     5761 atattgcgga tgcatagcaa ggtcgcctag atgataaaca tggtcggttt cttttattac
     5821 tgcattccag ctagtgatca actgttggtt catttcatcc acatttgcaa agagccgcgg
     5881 agcaaagtcg ttttttccta ataagcgttc atggaaaaag tgcatatcac tgataaagta
     5941 attcatgtat cttttgcctc ccttttttct tagagtaaac aaaatcactt atctgagcaa
     6001 ctcataacat ctaaaaagaa aaggttgtaa atgagacgtg cagctttaag cgataagaag
     6061 caattttgaa aaatagtttt tcacaattta taaaattatt ccttattgcc gaaaggctgg
     6121 ccctttttat gagtatgcca attttttata tcgaatgctc aaaagatgag tgattttttg
     6181 gttgactatc atgtttgtgt actgaatagg tccagtagaa cagtattttt acgacttgat
     6241 aattaaggga tgaaattttc agaaatatga cgtataatgt aaaatactga aaagttgcga
     6301 ccttcaactc gtagatgttt cagaatctaa ttgaaatgat ggtgatatca tgacagacaa
     6361 cagcaaaaca cgcgttgtcg tagggatgag cggcggtgtc gactcatctg taacagcact
     6421 tttgttaaag gaacaaggat acgacgtgat cggaatattc atgaaaaatt gggatgacac
     6481 ggacgaaaac ggtgtatgca cggccacgga agattataag gatgtagcga aagtagctgc
     6541 tcaaatcggg attccttatt actctgtgaa ttttgaaaaa gaatattggg atcgtgtatt
     6601 tgaatatttc ctagccgaat accgcgcagg acgtacacct aacccagacg tgatgtgcaa
     6661 caaagaaatc aaattcaaag cattcttgga ttatgcaatg gatcttggcg ccgattatgt
     6721 agctactgga cattatgcgc aagtcactcg cgatgaaaac ggcgtggtcc acatgcttcg
     6781 tggagtagac aataataaag atcaaactta cttccttagc caattatcac aagaacagct
     6841 ttcaaaaacg atgttccctc taggcggcat ggaaaaatca gaagtacggg caattgctga
     6901 acgcgcaggc cttgcgactg ctaagaaaaa agattcaacg ggaatctgct ttattggtga
     6961 aaagaacttc aaacaatttt tgagcaacta tctgccagca aaaaaaggaa atatggtcac
     7021 tcttgatggt gaagtcaaag gacaacatgc tggcttgatg tattatacga ttggtcaacg
     7081 tcaaggacta gggatcggtg gcggaggaga ttcgcaagaa ccctggtttg ttgtaggaaa
     7141 agatctagcg acaaacacat tatatgtggg gcaagggttc catcatcctg cattgtatgc
     7201 cacaagcttg gatgcaagtg aaatccactt tacgacgaat gaaccaatgc caaaagaatt
     7261 caagtgtaca gcgaaattcc gttatcgtca gcaagacgtt cctgtgacag ttcgcttatt
     7321 ggatgataat cgtgcagaag tcgtattcga cgaacctgta cgtgcaatca ctcctggaca
     7381 agcagttgta ttttatgatg gaatggaatg tctgggtggt ggtttgatcg accacgcata
     7441 tcaagaaaca aaagttttac aatacgttta agcgatatta aatgaatcaa aacagctatg
     7501 gctaacaaaa gaaacacctt ttcttttgga tagcatagct gtttttttgc aataaaatta
     7561 atttttattc attttatact tgaaatactt gtgtaaaaag ttcataatat ccaataatat
     7621 gaataaagtt gctttttgtt aagcaagagc agttgcaaaa tagaagggaa tcttttgcaa
     7681 gtgatttgtt caattttata agtgagggtg atggaaatgg aaaagaaatc gacgggtatt
     7741 agcaaatggg gactggttgc tctggttgtc agttcgtcaa tcggaagcgg tgtgtttggg
     7801 atcacaagtg atctggctag ttctgcagct ccagggccag cgatcctttc atggataatc
     7861 gttgggattg gtattttagc tttggtatta tcgctgaatc atttaggaga aaagcgtcca
     7921 gatctagatg gtggtatttt cggttacgcg gaagcttcat ttggtaaatt aggtggcttc
     7981 atcagtggtt ggggttactg gttgtctgcg tggctgggaa atgttgcttt tgcaacgatg
     8041 ctgatgagcg ccatgggaga atttttcccg atattcaaag gtggacaaaa tgttccttct
     8101 attttatttg caagtatttt tatttggggt ctgactttat tagtaaacaa tggagttgaa
     8161 ggagcgagcg tcatcaatac aatcgtgacg atctgtaaat tagtgccatt attcctattt
     8221 ttaatatttg tatttatcgc atttaaagtg aatatattca ctgctgattt ttggggtaac
     8281 gtatctgata atctagtgaa tggaacaggg cagcaaggaa cgatctggtt gcagattaaa
     8341 ggttgtttga tggttatgat gtgggtcttt gtagggatcg aaggcgcaag tgttttagcc
     8401 aaccgcgcga agaaacgttc agaagcccag caagcatcga tcattggtct gctttgttta
     8461 ttgtttatct atatccttgc ttcgatcttg ccatacggcg tcttatctca agaagagtta
     8521 gcaactattt cacaacctgc tatggcaaat attttaaaag gaatcgtggg aaattgggga
     8581 gcagcgctga tcaatatcgg cttgattatt tcgatcattg gttcttggtt gtcttggacg
     8641 atgctcccag cagaaacaac gatgttgatg gctcgagacg gtctgttgcc gaaaggttgg
     8701 ggaaaaacga ataaaaaaaa tgccccaact tattcgttag ttgttactgc aatcttgact
     8761 aatctattct tattgacttt cttagttact gattatgcct atcaatttgc ctattcttta
     8821 tgtacagcgg caattttgat ttgttatcta cttgtcggga tctatcaagt ccagttttct
     8881 ttaaaagaag gaatcaaaaa acaagtagtg atcggtggga ttgctgtagt ttttgaattg
     8941 atgggcatca ttttagcagg attttcttat gtccttttat gtagtattgc ctatcttcct
     9001 ggtttttgct tctactggat cgcttgtcgt gaaaccaaac atcaaattac gggaaaagaa
     9061 aaagtaatga ttgggctgat ttgtgtggcc gcactgcttt caatcgggtt gcttgctgta
     9121 ggctggatca agatataagg gggaaagaaa catgaaaatc gcaaatttcg gtgtagaaga
     9181 atggctaaat aaatgggaaa agaaagcggt atatgatatt gcacaaagct cgatcgaagc
     9241 attgacatta gacgaactcg ttggcttaga tgggactagc gtaagtaaat ttttcgaaaa
     9301 acgcaaaaac gaaccattgg actatggctg gatcgaagga tcagatgttt tcaaacaaga
     9361 agtcgcttct ctttatcaaa ccgtcgatcc ggaaaacatc ttacaaacga atggagcgac
     9421 tggtgctaat cttttagctc tatacgcgtt agtggaagca ggagaccatg ttgtttcgat
     9481 gcttcctact tatcagcaat tatatgacat ccccaaatct ttaggtgccg ctgttgactt
     9541 tgttcatttg aaggaagaag aagattggca gtttgattta gaacagctag aagccttagt
     9601 caaacctgaa acaaaaatca tctgtttgaa tagtgcaaac aatcctacag gcacattgct
     9661 agatcggaaa actttagaaa aaatcgcaga aattgctcgt acagtagatg catatgtatt
     9721 gatagatgaa gtatatgcgc ctttaacgga taaaggtgaa ttcttgtcga tcgtagatgt
     9781 ctatgaaaaa ggtatagcaa ccaattccct atccaaaacc tactccattc caggtattcg
     9841 tattggctgg acagctactg gacctgagtt ggcagaagtt tttcgaaagt atcgagacta
     9901 cacgatgatt tgcggcggag tcctgtcaga tgatcttgct gtacatgcat tgaagaataa
     9961 agaaaaaata ttggcgcgaa acaaaaagat cattacagag aaccttgtga ttttaaaaca
    10021 atgggtagcc aatgaaccga aagttgaact tgtcgctccc aattacgtct ctacttcttt
    10081 cattaaatta gccatcgaag aagatgacca gacattttgt atcaatttat tagaagaaac
    10141 aggagtacta ttagtaccag gaagtgcctt tgatttaccg aagcatgccc gactaggtta
    10201 ctgttgtaaa aaagaaacct tggaaaaggg attattcctc ttatctgagt tcttgaaaaa
    10261 acaggcctaa acaaaaacac tgcaaggaca gtctgtgaca actgttcaag cagtgttttt
    10321 catttacgct tattgtctat gagaaggaga gtgccctgaa gctatggaga ctcctgaagc
    10381 tttttcttct gcagtttctt gctgatggat ctcttgtgta ttgtgtcgta caagcaagaa
    10441 gttgaccaaa acaagaagag aagtcgaaag aaagacgccg cgataaccaa atgcactgga
    10501 aacactggag ccgatcatag gccctactac gttaccagtt gcttggaaag attggttata
    10561 actgaagatc cgtccagcag cttgatgagg cgaatatttt gtgatcaatg cttggacagc
    10621 agggagcaga caagcatcag agataccaat caaaaaacgt aaggcagcta attgccaaac
    10681 attcgtgacg aatgccattg ggatataaac cagaatcgca aaacccaaac caatcgcaag
    10741 tattctttca ctcccaatct tgtcacctaa gcgaccaaat cttggagcgg ctatcaaagt
    10801 ggcaatccca ggtatcgaag caatgattcc gctgatcaac gtaacattcc catgtccatg
    10861 cagcaattgg cgaatgtaca gactgatgat tggactaatg gaattgttgg atgcttggat
    10921 gatcatcgtt gtgacgaaca taccgatgac gacgtgtgga tatttcaagt ctttgaaaat
    10981 ctgtttggca gaaaccatgt tttctttttt cactgggaca aatttttcat ggacgaaaaa
    11041 cagactcaac aagaagacga aaaataagat gatccctgta atgaaaaagg tagggcgata
    11101 gccaaacgct gaagcagaga tcccgccaag aagcggtcca agtaatgttc ctgtaacaga
    11161 accagttgct aaggttccaa gaacttgacc acttttttct tttggcgtcc cagttgcgac
    11221 aagggcagtg gcattactga tataaccaga aaaaatccct tgaagcagac gcaatgcgac
    11281 taattgatac acactagtca cgcaacccat caaagaaatg acgattgcca ttcctaatga
    11341 agcacgcaac agcatcaatt ttcttccttt ttggtcggct aattttcccc accaaggaga
    11401 gataagcgta gtcaccaaga aagtagatga aaaagtcaat ccgctccaaa agttcaattg
    11461 tgacgtacta taattaccta acgtatctat gtaaagtgac ataaaaggca tgactaaact
    11521 aaatccgata ccagccataa atgttccaaa ccataagaca aacaagtttt gttcccaagt
    11581 ttctcttttc ctaaataatt tttcctttat tcttgcagcc aattagactc cttctttctg
    11641 aaaattttca tctacagctc tttattataa actacaatat gtagtttatc aatgaaagtc
    11701 tcttttttct ctttcttttt cgtggtaaac tgaaagagtg aaggatttat tttatgagag
    11761 gaaacatgga aatgaaaaaa agaaatctgt ctcaagagaa aatcatcgac tgctttcgag
    11821 aactagcaga agagatggat gttcagcaaa taacctttca gcatttagcc aaacgcttgg
    11881 atatcaaggc tccctcgctg tataaccatt tcaaaaatat tcgtgatgtc aaaacagcgc
    11941 taacagcgaa acttctgcag gaacttaatg accagctgcg acgggctctt gtgggaaaaa
    12001 gtggtcagga agcactgcag atctatgcag aaacttatcg atcctttgct ttttcaaatc
    12061 aagcagtgta tgagttactg atcagtgtac cgcataccaa tgaagctgtt ttattagatg
    12121 gaatccatga aacgaatcag atcattttgc agatatttga ggcttttcaa ttaacgaaag
    12181 aagaaaaagt tcatcgaagc agggaacttc gtagtatgat ccacggttat ctttccttac
    12241 gttttttagg atactttaca aaagaaccaa caatcgatcc agaagacagc tatttttgga
    12301 tgatcaataa ctttatcgct actttgcctt aaacaatcag attcatttgt aacaatttta
    12361 agatgttcat taaatgaccc ttttataaga gaaaattctt gcaactttct tgaaaagtga
    12421 ctaagatgag tctagaaaag ctgaaagaag gagcacgagt atgtatcaag taattaccat
    12481 gttcggggac aatgagcctt ggtggttttt tgaagactgg gaagaagata ttgaagaaga
    12541 agaaacattc gagtctttcg aagaagcacg acaagcgtac gaaaaacaat ggaacgaaat
    12601 ccatcaaaat tatgaatata tcaatgcaaa aagtaacttt ctcagcgcct tttggaatga
    12661 cggagatgag cgctggtgtg aagagtgtga cgatgattta cagcaataca agggacttgc
    12721 gttgttgaaa aatcatcagc ccatcacagt agaaagcaga aaggaatttt atgaaacaac
    12781 taattctagc ggaaaaacca agcgttgcga aagacctaag caaggtgctt ggtgctaatc
    12841 aaaagcacaa aaattattat gaaggaccca aggtgatcgt tacttgggcc cttggtcatt
    12901 tattaggatt gaaaatgccg gaagatctga ataaggaatg gcagagctgg caaatggaca
    12961 cgttgccaat gatccctaaa aaactaggaa tcaaaccact tcctaaaaca ggacatcaat
    13021 taaaagcaat caagcagctg gctcagcgaa aagatgtctc agaagcagtc attgcgacag
    13081 atgccggaag agaaggggaa cttgttgctc gctggatctt agagtatgtg catttcaata
    13141 aaccagtgaa gcggttatgg atctcttctc aaacagataa agcaatcaaa actggattca
    13201 agcaactaag acctgcgaaa gaatatgatg cattgtatga atctgctctt gcccgggcaa
    13261 aagcggactg gctggtagga ttgaatgtga ccagagcctt aactgtgaag tacgcggata
    13321 atttatcagc aggccgtgtg cagacaccga cactagcatt agtgagagac caagaaaaga
    13381 aaatcgagca gttccgtcca caaacgtatt tcacgatcac gttgcaagct ggaaaagaac
    13441 aagccaaaat gcttcaaaaa aatcaatttg ctttaaaaac acaagaagaa gcacaaaaac
    13501 ttgttcaatt gatcagtaca agcaaaggaa ctgtcacaga agtccaagaa aaattaaaaa
    13561 cggaatctgc gccattgcct tatgatttaa cagagatcca aagagaagcc aatcaacgct
    13621 atggctattc tgcaaagaaa acattaagtc ttgtacaaag tttgtatgag acccataaga
    13681 tcgttactta tccgcgtaca gacagcaaat atctgacaaa tgacatgaaa agtacgatga
    13741 aagaacgatt gcaagcagtc agcgactttg ctcctgaggt caagcagtat ttgaaaaacg
    13801 gagccatcgt ccaacagaca aaagtattcc aagacagtaa agtgaccgat caccatgcac
    13861 ttttgccaac tgaaaacaga gcacgttatg aaaaattaag taatgaagag cgaaaaatct
    13921 atcaaatgat tgtctctcgt tttttaggac tgtttgctca accccataaa gtcagccaaa
    13981 caaaagttac ggttgaattt ggtaaagaac gattcatttt tcggcaaaat cgtgtcgtcc
    14041 aagcaggatg gaaaggcgaa actgaaactg agacggaaac agtcaaatgg gaaaaaggaa
    14101 tgagaatcaa tcccgatttc gccatcaaaa aagaactatc tgcaccgcct aaaccattgt
    14161 cagaagcaag cttgcttggt caaatggaaa aatacagtct gggaacccca gctacgcgtg
    14221 cagagattat cgagaaacta atcaaatctg aactgatgga acgtacccct caaggactgc
    14281 aagtatctcc taaaggtaag caattattag tattggtaaa tccctcttta gtaacaccag
    14341 atttgacaga aagctgggaa aaagaccttg aagctattgc tgcaggaaag aaacaagcag
    14401 gaaccttctt gaatggaatc gaaaaagaaa caaaacgctt ggtgaatgag atcaaatcaa
    14461 gcaaacaaga atatcaagat ttctcgatta cccaaaagaa atgcccagaa tgtggggcca
    14521 atctgcgcga gaaaaacaca cgtgacggca aaatctatgt ctgcaccaat tcagattgca
    14581 actaccgcag aagaaaagat ccaaaagtct ctaatcatcg atgcccgcaa tgtcaccgga
    14641 aaatggaaat catcgaaggt aaaaacggtg cttatttccg ttgtaaatac gatggaacga
    14701 cagaaaaatt gcttgataaa aaagagcaga agaagaaaat gaccaaacat gaagaacgac
    14761 gcttgctgaa aaaatatgca caagcagaag aaccagaaga aagcccatta gcagcagcac
    14821 taaaagcagc aatgaagaag taataagata tgaaagaggt tgtgatagaa gtggtcagct
    14881 tcaagaaata agaaggaatt tacgaaaatt gcttctcaaa tttttgtgaa atttcggctt
    14941 atttccgaag aagctacttc tggaacacca tttattcgga aaaaaggact gtgacaagac
    15001 ttttgtcaca gtcctttttc atttaaaaaa atgtttactt catttgacgt atattattgg
    15061 tgaggcttca attcatttgg cttcactttt tctgtttctc ttatgatata aacaggacgc
    15121 tgtttcgttt ctaagaagat tttcccaata tatttcccga tgattccaag acataacaat
    15181 tggattccac caatgaacag gaagatcgag accatggaag gccatcctga tgtaggatca
    15241 ccaaacaaca atgcccgcaa aataatgaac aaaattgcaa taccagaacc gacacaagac
    15301 aaagcgccga taaatgaagc aatcgttaac ggtgcatccg agaagttcac gataccatca
    15361 atcgagtagt tgaataattt ccagaaggac caagatgttt cgccagccac acgttcgcgg
    15421 ttttcataag aaatatattc tgttttaaat ccgacccagc taaataatcc ttttgaaaat
    15481 cggttgtatt ctgttaattc taggacggca tccaccattt gacgagtcat caagcggaaa
    15541 tcacgagcgc catcgaccat ttctgtatcg ctgattttat tgatcaactg gtagaatttt
    15601 ttcgcaaaga aactgcgtat cgcaggctcg ccttcacgag tcgaacgccg ggtgccgaca
    15661 caatcgatat cagaagtagt gatccggttg atcatctcag gaagcagttc gggcggatct
    15721 tgcagatcaa catccatcac ggtcactaaa tctcctgtag aagcttgcaa tccagcgtac
    15781 agagccgctt ctttcccaaa atttctggaa aatgagacaa agcggacata aggataagct
    15841 gaggctagct tcttgattgc tgctaacgta ccgtcttttg atccgtcgtc aataaacaaa
    15901 tattcgaact catgggcacc tttgaatttt tcaacctcat caaaaaaaag aggaacagtt
    15961 ttttcttcat taaaacatgg gacaacaatt gatactttca tcgtttctat cctttcaatc
    16021 gtatgtttat atatggttta cattcaaaat ttaataaacc ttaaataaca cctttaaaat
    16081 ttgattttac gtaaaagaac aggtattctt tttgtaaact aacgtattta gtttaccatg
    16141 tttcctcggt ttatcaaact tttaagaaca atttttatta gttggcaagt agttttggat
    16201 agagaaaaag gaggaagttc cagtctattg actaggagca aacaaaatga aaatacacac
    16261 acctatcggc tttttatggt tagatgcaga tgaatccgga ttgactgctg tatcttttca
    16321 gccaatttca gaaagcgaat cagcgaatca gcgaatttta acgcgcacac aagaacagtt
    16381 tgaagaatat tttgcgggaa aaagaaaaga atttgatgtt cctttatcta taaagagagg
    16441 tacgcctttt cagcaaaatg tctggaaagc tttgtcgacc attccttatg gagaaatccg
    16501 aacgtataaa gaagtagccg ttgctgttga tagtccgaaa gctgtgcgag cgattgggca
    16561 ggcaaatcga accaatccat tgccaatcgt tattccttgt catcgagtga tcgggcaaaa
    16621 tggaaaactg acaggatata tgggaaacgc aatagatggg gttgaaatca aacgccaatt
//
LOCUS       DAZAFF010000060        16627 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000060 DAZAFF010000000
VERSION     DAZAFF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.060"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..161)
                     /locus_tag="ACG9BB_001876"
     CDS             complement(<1..161)
                     /locus_tag="ACG9BB_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN2837808.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINR"
     gene            complement(163..360)
                     /locus_tag="ACG9BB_001877"
     CDS             complement(163..360)
                     /locus_tag="ACG9BB_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFN2837809.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(754..2481)
                     /locus_tag="ACG9BB_001878"
     CDS             complement(754..2481)
                     /locus_tag="ACG9BB_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFN2837810.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2474..3667)
                     /locus_tag="ACG9BB_001879"
     CDS             complement(2474..3667)
                     /locus_tag="ACG9BB_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFN2837811.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3854..4498)
                     /locus_tag="ACG9BB_001880"
     CDS             complement(3854..4498)
                     /locus_tag="ACG9BB_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2837812.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4592..4726)
                     /locus_tag="ACG9BB_001881"
     CDS             complement(4592..4726)
                     /locus_tag="ACG9BB_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFN2837813.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4728..6860)
                     /gene="feoB"
                     /locus_tag="ACG9BB_001882"
     CDS             complement(4728..6860)
                     /gene="feoB"
                     /locus_tag="ACG9BB_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFN2837814.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6857..7330)
                     /locus_tag="ACG9BB_001883"
     CDS             complement(6857..7330)
                     /locus_tag="ACG9BB_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFN2837815.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7731..7955
                     /gene="nrdH"
                     /locus_tag="ACG9BB_001884"
     CDS             7731..7955
                     /gene="nrdH"
                     /locus_tag="ACG9BB_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFN2837816.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8114..10273
                     /gene="nrdE"
                     /locus_tag="ACG9BB_001885"
     CDS             8114..10273
                     /gene="nrdE"
                     /locus_tag="ACG9BB_001885"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFN2837817.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10323..11288
                     /gene="nrdF"
                     /locus_tag="ACG9BB_001886"
     CDS             10323..11288
                     /gene="nrdF"
                     /locus_tag="ACG9BB_001886"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFN2837818.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11377..12516)
                     /locus_tag="ACG9BB_001887"
     CDS             complement(11377..12516)
                     /locus_tag="ACG9BB_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFN2837819.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12825..13538)
                     /locus_tag="ACG9BB_001888"
     CDS             complement(12825..13538)
                     /locus_tag="ACG9BB_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFN2837820.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13792..14493)
                     /gene="nagB"
                     /locus_tag="ACG9BB_001889"
     CDS             complement(13792..14493)
                     /gene="nagB"
                     /locus_tag="ACG9BB_001889"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFN2837821.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14727..16259
                     /locus_tag="ACG9BB_001890"
     CDS             14727..16259
                     /locus_tag="ACG9BB_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN2837822.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16450..>16627)
                     /locus_tag="ACG9BB_001891"
     CDS             complement(16450..>16627)
                     /locus_tag="ACG9BB_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2837823.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5160 a         3425 c         2875 g         5167 t
ORIGIN      
        1 aatctattta ttaaaggact gggatctaat tcatattttt gtttttctaa ttttaaatat
       61 accttttgta aaacctcaag aatatcagaa tatttaggat ctttacttat agatatatca
      121 ttatataatt catgaacaat ttgcttagca ttttttttca ttttagcact tccctggaat
      181 tgctccacct aaaaaaccac ctatagacat tcctgcaata caagtagttg ccttggccca
      241 atcgaccgta catttatttt tagtgcaata cactccattt ccataatatt ttccactatg
      301 agtggtaccc ccatagataa gttgtgtctc tttaatagac aaaattttta aatgtttcat
      361 aatctaatca cccctaaatt aatgttttta gactattatt ctaacattca ttaaatttca
      421 aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg aataagaatt taatcacttt
      481 taagtatata aactatgtat attctttaat attttttgag aatgcacttc gtacataata
      541 aactttaatc acattagtct ttcataccta cgatcgatct ctttgactcg cacccataaa
      601 ttcataatag tttgtcggcg tctttgaacg tttcgaagat gagctacgca ttctcttttt
      661 ttatagacaa aaaagataga gacgaacatg gagcttccaa atttggatat ttgatccaat
      721 tttggaagct ccatttattt cgtgtctgtc tttttatttt ttccagcttt cgactaattc
      781 ttggttctct ttgatccagt cttttgctgc ttgctgtgga tcttttccat tgttgatttc
      841 caacataact ttttccatat ctttttctga ccaatggaaa ttgtctaata ctttgtaagc
      901 ttctggctgg tcttctttca atccttttcg aaccatcgta tggatctgct cttcgcttcc
      961 cattccgttt tcaggatctt ctaagtattt cagatcatat ttggcaaaca tccaatgagg
     1021 tgtccatcct gtaactacaa ttggttcgtg ttttttgatc gcttgaccta gtgcaacggt
     1081 cattgctcct gaagaagatg tctctacttt ccagtctttc aagttgtcat atttttggat
     1141 cgtgttttca gcagccgtaa ctacaccagc tcctggctcg attcctatga ttttcttacc
     1201 tgcctgatcg gttaactcat cgatcgaatt tacatccatg taggctggca ctgctaaacc
     1261 aacttttgct cctgtcaaat tggcacctag gtcttcgact tgatctttat attgtgcata
     1321 ttgtgaacca tgagttttag gtagccaagc tgaaaccatg gcatctgact cgccatttga
     1381 aacagatttc cacatgattg aattatctaa tggcgtcatc gttacatcat agcccatttg
     1441 tttcaatact tcaccgacta catttgttga agcaacttct gtatcccatt caacataaga
     1501 taagttgata cgttttgttt ctttggcaga tgaaaaactc cctataccaa tcgcacctaa
     1561 gatcactact actacagcac caatgatcag tccttgacgt tttttacttt tctgtgttgt
     1621 tgctcctgca gcttttttat tgtttggctg gttcaaatgc tgtgtaaaac ggtcaatgat
     1681 gattgctaag ataactaatg caaccccgtt gacaaatcca ttcccgactt gtgctctttg
     1741 caatgctgat aatacgccgc gtcctaagcc gggtgcaccg atcatagagg caataacgac
     1801 cattgaaagt gacagcattg ttgtttgatt gatccctgcc atgatcgtac ttttagctaa
     1861 aggcaattct agtttgaata gtttttgacg acttgtgctc ccgaatgaat cagaagcttc
     1921 tactaattct ttaggaactt gtcgaatccc taaattcgta aaacgaacgg ttggtggcag
     1981 agcaaagatg actgaggcaa atacccctgg aaccatcccg ataccgaaaa atgcaactgc
     2041 tggaatcaaa taaacgaatc ctggcattgt ctgcataaag tcaaggatcg gtttgataat
     2101 actttgggct ttactgcttt ttgccatcaa gatccctaat ggtaccccaa tgatgatcga
     2161 gatcacacta gataaaagaa caagtgtgac agtattcatc aaatcattcc atagaccttg
     2221 gttataaata aaccatagac cgattaatgt gaacaacgta agtcctggac gtttcttaga
     2281 aataaagaaa gctgctaccg tcaataacaa gataaacaaa ggagctggaa tcactaacag
     2341 taaatttgta atccccgaca tcactgcctg tccaatcgtt tgtaaaaatg aaaataggcc
     2401 agaaaaagtt gtcgtaaacc attctgtgat attttctacc cagtttgcca ctggtaattg
     2461 atagttcaat agattattca tgttcactca cctcgcttgt ttctgctaat gcttcaatca
     2521 cactacctcg gatgacgaca ccttttaatt ttccatcctc tacaactgca agtggagtcg
     2581 gtgagtcata aataatattg aaaatatcac ttaccaccat gtctttccca atcaccgtca
     2641 cattttcatc taggaagtcg atcaatggac gtttttgctt cctagcttct aacgcttgat
     2701 ctgcagtgat gatccctttc aggtgtctct ttctgtcaac agccatcagc atgctgactt
     2761 cttcattacg catccttgtc aatgcaacgt ttggtccatc agattcgata ttcgtcgtca
     2821 atgcaggtac catgatgttt tgagccgtaa gcactttgga acgatcgact tcttccacaa
     2881 attcacgtac ataatcattt gctggattgg tcaatatttc ttcacctgtt ccgatctgca
     2941 tgatttcgcc gtctttcatc aaggcgattc tgtccccaat ccgtaaagct tcattcaaat
     3001 catgggtaat gaaaataatc gttttttgaa cattggcttg caggtccagt aattcgtctt
     3061 gcatttctcg ccggatcagc ggatcaagag ccgagaacgc ttcgtccatc agcaagatct
     3121 caggatcatt tgctaaagct ctggctaggc caacccgttg ttgcatccca ccagataact
     3181 gactaggata ttggtcttta aatgaaagca aactagaatt ttctaatgct ttttctgctt
     3241 tttcttgtcg ttcttcttta ggtacaccac ggacttctaa cccgtattct gtattttcca
     3301 agatcgttcg atgtggaaac agaccgaagt tttggaaaac catattgatt ttatgacgac
     3361 ggacttctcg caattcttct ttgcttaact tcgatacatc ttctccatca atatagatac
     3421 tgcccgaagt tggctcaatc agacgattca atagacggat caatgtggat ttcccactcc
     3481 cggaaagccc catgatcacg aagatttctc cttcttttac gtcaaaattc acgtcataca
     3541 caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt cggctggtga tcattcatca
     3601 tatctaaagc ggcttgtgtc tttttaccaa atatctttgt taaatgtttt acttctactt
     3661 ttaccaaaca aattctcctt ttttatgtaa attcattttt ctttctgaaa aaagtgacca
     3721 cactatgtta acatcatgta gtcactttgt caaaaatgaa agaaatttgc ttgtccaaac
     3781 tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta gccaagttaa tattttttta
     3841 atatttcaca caatcattct gcaagctgat gctccgggta aaaaaagttt gtcatacgct
     3901 gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc tgccgtaaat ttagcatagg
     3961 ggccaggtga aacaagcagt tcttcattat gagaaattgc gataactgtg gcgccagtcg
     4021 cctgccagat attcaactcg ctaattgaac ggtccagatg cattgctttt tctgataaag
     4081 ataattcaaa cggaagtaaa ggattcatct tatgtattcg cttcgtttga ttgaccagct
     4141 gatccaataa ttgagaaaaa tgattcagct catcttgttg cttctccaca cttgccatga
     4201 tttctgcttt tgtatcttgg atggattgta catcttgata ttgcaatagg aaatcttttg
     4261 ctttttcctt cgaagcaaca aatgccccac ttccatgccg tacttccatg attccaagat
     4321 ctaccagcac attgatagcc ttgcgggtag tctcaggaga agcattgtac gtactggcaa
     4381 gtgttgagcg ggcatggatc ttctcaccca ctttatatcg gttttcaacg atacgttcgg
     4441 ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat ttttttcttt ccagacattt
     4501 tctttcctct ttcttttttt actttccctc atcttactat gctctagcaa aataagcaaa
     4561 aaaggggaga atagcctctt gcaacgactg atcatcgctt gatctgttcc ttttttactg
     4621 ggcaagctgt ttgacagtca tcacaattct taccggattt taaacgagta tagacaattg
     4681 cgcctgcact tccaaagatt aacacactta ataagaatgt cgccatccta ttcctctcct
     4741 tctaatgaca atatagtata aaactggttc ttggattttg gctgacggaa caaaacataa
     4801 acgagtccta tcatcagtaa aatagcaaga tacgtcccta acgctacctg tccaccctcc
     4861 aataagtgtc ccaactgata aatcacaaaa ctaactaaat aagctaaacc gcattgatag
     4921 cctatggcga tccaagtcca tttcttggca ttcatttccc gatggatagc cccaattgcc
     4981 gcaaaacaag gcgcacaaag taaattgaag gctaacaatg aataagctgc tgcgggagta
     5041 tatgtgtgtt gtagcacttg ccagatttct ctgccatttt cggatacttc cgcatgacca
     5101 aacaaaatac caaatgtgcc aataatattt tctttagcga ccaatcctgt aatcgctgcg
     5161 actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa caactgccag gattcgcccg
     5221 aaaaaggcta agatactttg gtcttcagaa acaggttgag cataaaaatt ataagaagag
     5281 ctgaaccaga tcacgatact agaaacaaag ataatcgttc ctgctttttt aacaaaagac
     5341 ttgctgcggt cataggtttg tcttaggacc gctgaccatt gaggaaaatg atatgcgggc
     5401 aattccatga taaaaggagc tgtatctcca gcaaacaaac gtgttttttt caagctgatt
     5461 ccagagaaaa taattgcagt cataccaaga aaataggcag aaggagctac ccaagattga
     5521 tgaggaaaaa aggcccctgc tactaaggca ataatcggca actttgcaga acaaggcatg
     5581 aaggttgtga ccatgatcgt caaacgacga tcttgctcgt tttcaatcgt gcgactggcc
     5641 atgacgcctg gcactccaca tcctgtagca atcagcatag gaatgaagga ttttcctgaa
     5701 agaccgaatt tcctgaaaag tcgatccatg acaaaagcga tcctcgccat atatccgcag
     5761 tcttccagaa agctcaagca taaaaacaat ataatcaatt gcggtagaaa tcctaacact
     5821 gctcccacgc ctgcgatgat gccgttcaag atcagctcct gcatccatcc cgctacttgc
     5881 cattcagcta acaatctgcc aacatgttct ggtaaccatg taccaaacaa tgtgtcattg
     5941 atccaatctg tccccattgt tcctactgtt tgaatagcca gataatagat cagccacatc
     6001 acaaatgcga agatcggcaa ggctaaccaa cgattcgttg caatctgatc gattttgtca
     6061 ctcatcgata attggaaaga atcatttgac acaacacaga gtgtacatag ttgggtaata
     6121 aattcataac gttcattgac cagaatcgtc tcactatcgt cgtccaatag tttctctgtt
     6181 aactggatca gttgctcgat ctctttttgc tgagaaactg ctatctcgta atcctctctt
     6241 accttctgat ctcgttcaaa atatttcatt gcataccagc gtttcttgcc gctttgttct
     6301 cctagtacat cttcaatttc atgtaaagct gcttctaaac gtggatcata tgtacaggga
     6361 acaggaatag aaggccgctt ttttgcagac tcctgaatct tttcgattag tttttcgatt
     6421 ccttgctttt tcaatacgct gattggtaaa actggtacac caagaccata ggaaagcttt
     6481 tctagattga ttttttttcc ttcttttttg atgacatcca tcatattcag accaatgatc
     6541 ataggaatgt ctaaatccaa taactgcgta gttaaataaa gatttcgttc taaattggta
     6601 gcatcaataa tgttgatgat cagttcaggc tgttcctgaa ccaaataatc tcttgcaatc
     6661 acttcttctg gtgtataagg agacaaagaa tagatgccgg gtaggtcctg aatatgaatg
     6721 ttcggatttt ttttcaacga tccttctttt ttttctactg ttacgcccgg ccaattccct
     6781 acatattgat tcgaaccagt caagagattg aacaagctag ttttcccgct gtttggattt
     6841 ccgactaacg cgatattcat tctttttcct ccctttgtat caagattagt tcggcctctg
     6901 actttcgtaa cgtcagttca taccctcgca ctgtcagttc gattggatct cccagagggg
     6961 ctagttttcg gataactatt tttacatttc gcgtcaatcc catatccatt aaccgtcgct
     7021 tcactgcgcc ttgtccgtgg attgctacga cgatcccagt ctctcctgct tttaattgat
     7081 caagagaagt ccagctttca gaggaagcag tctttttatt gacaaagatt tgacctagta
     7141 tttcccgact tactgccact cgatgacttt ttaccagtaa aatggcattg ttgcctgata
     7201 aattgacgac gacgatttta cttccggcaa cgatcccgat attttgcaaa tggcgttttg
     7261 ttttttcttc tgtttcgatt cgttcgactt gataaacgaa attctgcttt gcctgcgcta
     7321 aggtctgcat attgattcct ctcttgtgaa ataaatcatg tattaaaatc attcattata
     7381 tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat cattctcaca tagaatactc
     7441 tcggtaattg agaatgtcaa ttatacttgt gatattatga gcgaatcttt agtattgata
     7501 gataaaaatg agaaaaaaat atttcttcat agttttccac aattttttct ctattaaatt
     7561 tgctattttc ccacacgata cttgttttca gctctttttt caaaaacaca aaacttagta
     7621 ttccacagtt tccacaacac aatatgtagt gtttgttgtt tgactttttt actaagagtt
     7681 ttatactatc tattatagat aataagaatg tgttagagga ggcactcacc atgaacgtaa
     7741 aaatcttttc taaaaataat tgtatgcaat gtaagatggt caaacggttt ttaacagaaa
     7801 atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc tgaagcagta gactggttga
     7861 aagcacaagg attccaaagt gttcctgtca ttacttctga tgcagaaaca gttatcggat
     7921 tccgtcctga tcagttgcgt aaacttgcca gctaacatga ctaaaagaat caaaacgtga
     7981 tacatggatt tgaagggctt gagacagcac cagtttttgt cgcaaacttc caaatccttt
     8041 tttaaagctt aaaaacaatg caatgatttg caatcaagca tcatcaaata cgaaatgaag
     8101 gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc agttatttca agctgaataa
     8161 tgaaatcaat cggccagtca atggtcaaat ccctttgaat aaggataaag aagctttagt
     8221 tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact tttccatcta ttatggaaaa
     8281 aatcaattac ttgatcgatc aagaatatat agaagcagaa ttcattcaat tgtattcacc
     8341 atcatttatt gaaaagctat atcaattttt ggacgagcaa catttttctt tcaaatcttt
     8401 tatggctgct tacaaatttt attctcaata tgcgttgaaa accaacgacg gttccgctta
     8461 tctggaaact tatgaagatc gagtagcctt caatgcgctt tactttgcga acggggatga
     8521 agaattagca cttgctcttg cagacgaaat gatccatcaa cgatatcagc cagctacacc
     8581 ttctttcttg aatgctggac gtaaacgtcg gggcgaattg gtgtcttgtt tcttgactca
     8641 agtcactgac gatatgaact ctatcgggcg ctcaatcaac tctgctttgc aattgtcccg
     8701 tattggtggc ggcgtaggga ttactttatc aaatcttcgt gaagccggcg ctcctatcaa
     8761 aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg aaattattcg aagatagttt
     8821 cagctattct aatcaattag gtcagcgtca aggagctggc gtggtttatt tgaacgtttt
     8881 ccatccagac attattgctt tcctttcagc aaaaaaagaa aatgcagatg aaaagattcg
     8941 cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa ttttatgaat tgacacgtaa
     9001 aaacgaagat atgtatttat ttagtccata tagtgtcgaa aaagaatatg gtgtcccatt
     9061 ctcttatgta aatattacag ctgagtatga tcatctagtt gcaaatccaa acattcgcaa
     9121 acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca aaattacaac aagaatctgg
     9181 ctatccttac gtaatcaata ttgatacagc gaaccgcgag aatccaatcg atggcaagat
     9241 catcatgagt aatttatgtt cagagatttt acaagttcaa acgccttctg tgattaacgg
     9301 caaacaggaa tatgaagtat taggaacaga tatctcatgt aacttaggct cgacaaatat
     9361 cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg cgtacgatga ctcgtgcatt
     9421 gacatttatt acagatgcat ctgatatcga tgtcgtacca ccgatccaaa acggaaataa
     9481 gctgaatcat acgattggat taggtgccat gggcttacac acttatcttg caaaagaaca
     9541 aatggaatat ggctcagagg attcattaga ctttacaaat atttacttca tgctgttgaa
     9601 ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa agaaaacaag ttttccataa
     9661 ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa aaatacgtaa ctggagaatt
     9721 ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc atttttatcc caactgctga
     9781 agattggaat gctttgaaac aagcagtaat gaaagatgga ctgtatcatc aaaatcgctt
     9841 ggcagtagca ccaaatggct caatttcata tatcaacgat acgagtgcaa gtattcatcc
     9901 tatcactcgc atgatcgaag aacgccaaga gaagaaaatc ggtaaaattt attacccagc
     9961 gccttatcta tccaatgaga cgattccgta ttacacttct gcttatgata tggacatgcg
    10021 taaagtaatc gatgtttatg caacggcaca acaacacgtc gatcaaggca tgagtttgac
    10081 tttattcatg cgttctgaaa tcccagaagg catttatgaa tggaagacaa caacgaagca
    10141 aacgacacgt gatttgaata ttttaagaca ttacgctttc cacaaaggaa tcaaatccat
    10201 ttattacgta cgtaccttta ctgatgatgc agaagaaatc ggaagcaatc aatgtgaaag
    10261 ctgcgtgatc taatatcata gatagattcg cttattcata ttttaattaa aggagcattc
    10321 tcatgtcaga aacatattat gaagcaataa actggaatga aattgaagat atcattgata
    10381 aatccacttg ggagaagctg acagagcaat tttggctaga tacacgtatc ccactttcaa
    10441 atgacctaga tgattggcgt acattgtctc aattggaaaa agatacagta ggacatgtat
    10501 ttggcggttt gacactgcta gatactgtcc aatctgaaag tggcatggat cagttgcgta
    10561 aagatgtccg cactccccat gaagaagccg ttttgaacaa tattcaattc atggaatccg
    10621 ttcatgccaa aagttattct tctattttta gtacactgaa tacgaaaaaa gaaatcgaag
    10681 aaatttttga ctggacaaat acgaatcctt atctgcaaaa aaaagctgag cgtatcaatg
    10741 aaatctacaa acacggtaca ccgctagaaa agaaaattgc cagcgtcttt ttagaaacat
    10801 tcttgttcta ttcaggtttt tatactcctc tttattattt aggaaacaac aaattggcta
    10861 acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc tgtacatgga acctacatcg
    10921 gttacaaatt ccaattagga ttcaacgagt tacctgaagc agaacaagaa acgttaaaag
    10981 actggatgta caatcttctt tatgagcttt acgaaaacga agaacgttat acagaagaat
    11041 tatacgatcc aatcggctgg acagaagaag tcaagacatt cctacgctat aatgccaaca
    11101 aagcattgat gaacctaggg atggatccac tctttccaga tactgccaat gatgtcaacc
    11161 caatcgtaat gaacgggatt tcaacaggca caagcaacca tgacttcttc tcacaagtcg
    11221 gaaacggcta tttgttaggt catgtagaag cgatgaaaga cgacgattat ttgattggat
    11281 tggaataagg atttatttta attcaaaaat ttttcgaaaa ataaaaagag tttagcgtac
    11341 agatatttta aatgtacact gaactctttt ttatgcttat tcacttgttt cttccagcac
    11401 ttttggactg tctaactttt ccgaaacatg ttccattctt ctcaataaat agattttccg
    11461 gatatttaat attatcgtct taatcactgc atagacaggt atgcaaatca gcatgcctaa
    11521 tattcctgcg acacttcctg ctccaatcaa taccaagatg atcgtcaaag gatggatatt
    11581 cattgatttc ccaaataaca acggcttgat caaatttcca tctaattgtt ggatcactaa
    11641 aatcgacaac gccatataca aagcttgaaa tggagagata aacaaaccga caatgattgc
    11701 tggtaccgct ccaataaaag ggccgacata cgggataata ttcgtgattc cgcaaatgac
    11761 cgcaagcaac aagctataag gctgatcgaa tattctcatt gccaaaaagc tcattacacc
    11821 aataatcaaa gcatctaaga tcgtactgct gatataggca gataatgttt cgtttaattc
    11881 ttttaccgtt tggcgtaact ctttacgaat cgctttcgga aagaagtgag acaacgcatc
    11941 aataaatttg tgcccatcct tgaacataaa tagcaaaata aatggaacgg taaataacaa
    12001 aataaaaaat tgcatcataa atgaaatgat ttttgagaca cttgcagata aactgactaa
    12061 tatgatattg aacaaattac taagagataa atttgctgat gctaattgtt cttccaaatt
    12121 gaaattttta aactcttccc gctgcgccaa ttcatttaac catttgcttg tttcatctgc
    12181 atacattggc aagctctggg tcaattgaat tgtctgttcc attaactgag gcactacatt
    12241 catgacagtt aaaacaacta aaatgattac gaccaaaaac gtcaataaaa aaccgaacac
    12301 tctcgggatc cgatgctttt cgagaaaaac aacgattgga tcgaacatat aatagataaa
    12361 accagcaaca agcaatggta aaaaaattgc cgaaagcatt ttcaatattg gactaaaaat
    12421 attcggcatt tgtaaaataa agtacacgcc gatgatcgtg agtactagct ccactgtcca
    12481 gaaaaataat tttgattgtt taaaacgtga ttgcatacat ctcctccaag gtcctttgat
    12541 acttctattc tatcatttaa caaagagtta cgcgctgaga ttttcatttg cttaaaaaaa
    12601 acaaaaaaaa taaacatatt ttaatgagta gacgagtact taaacaaaaa cacggtttgg
    12661 agtagtgaca aaaattatac tgcagtccct ttttccaaat aaacggtgtt caaaagcttg
    12721 catcttcgta ataagcccag ccaaacgaaa aatataagga actattttta tttggctggg
    12781 cttattactt gatgtaggac atctatttta caacctcaga tcatttattg cgcaatactg
    12841 actaaatgca actttgtgat atttttgtgt acggacaact cctcgatcac atcggcataa
    12901 gtgagccctt ttggaagatc aatcgtatag atatttttgt acactcggta gtcatcaacg
    12961 aattttacgt caaaattcac atcttcgatt acgatatttt tctcttcaaa ataatcggta
    13021 agaaaatctt tggtttcttt tctatggata aattggattt ctaacttttt agtcgtaggt
    13081 acatggatca cgcgtttcac tactgtgagt gcaatagcaa tcccgataaa actgatgatc
    13141 gcaatggaat agtaacccat gccgatggcg atccctaatc cagcaattgc ccataaagaa
    13201 gcggcagtcg taagtcccgt caccgattgt ttcgtgacga tgatcgttcc cgcgcctaag
    13261 aaaccgacac cactcaccac ttgagcaatc agccttgctt catctgaacg aatcgttcca
    13321 gcaagctgtg gatactctaa agcatctctt aatgcaccac tagcgatctc aacttggatc
    13381 aatgcaatga tacatgctcc catacaaact aaaatatgcg tacgcatacc tgctggacga
    13441 tttttatatt gtcgttcaaa accgattgct ccgccaaata gcatagcgat ccctaaacgt
    13501 aaaataatct ctggaattgt caataccata tcattcatct aaacaccctc cagtccaata
    13561 tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt tacctcttac gaaaaaagga
    13621 ctgtgacaaa atttttgtca tagtcctttt tatgaataaa cggtgttcaa tcagctgacc
    13681 ctcggtatta ctcaacaaac ggcaaaatat ggggagctat ttttcctttg tcttctctaa
    13741 tactcaggtc aaaacgctga tttcacaacc tctttagcct actattgaaa attacaactt
    13801 acttgctgct tcttcatcga tgatcacaac aacattgtcg tgattttgca atacacttgc
    13861 tggcaaatga ttcgttacag gtccttcaat catccctttg attgcatcag ctttgctttc
    13921 gccaaatgcc atcaagacga tttctttact tttcataatt gaaccgattc ccattgaaac
    13981 agcttttgtt ggtacatctt ctgctttatc gaagaatcgt ttgtttgcat tgattgttga
    14041 ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa gcaccaggtt cattgaaacc
    14101 gatatgtcca ttttgcccga ttcctaagat ttggatatcg attggatgtg catcgatgat
    14161 ttgatcatac tttttgcatg ctgcttccag atcttctgct tttccatctg gtacaaacgt
    14221 ttctttgaat ggttttttat caaataattg gacatccatg aaatgacggt agctttgttc
    14281 atctgaaccg cctagtccaa cgtactcatc taagtttaca gaagtcattt cactgaaatc
    14341 caaatcgctt gaagtcattt ctttatataa agtaactggt gtgcttcctg ttgccaatcc
    14401 taatactttt gcacctttgt tcataccttc tttgatgatt tcaaatgctt tttttccgcc
    14461 ttcttcagcg ttttttacac gaatgatttc catagtgatt gctcctttat attttggtat
    14521 agcccaatta tagcattctt tttttctttt tacaataatt agtatagacc aaattgcatt
    14581 tttttcattt tcttgacaat aaataggaat ccgtgttagg tttcataaga tggttcaatc
    14641 aaacaatcca tgttgcatga aaaccaagca agtgattcac tgtggacgct ttatgtctgc
    14701 agttttttta tgaaaaagga gttattatga gtatactgac attaaacaac attacccagc
    14761 aatttggcga caaaatcctc tatgaaggtg tcgatctgca attaaatgct ggcgaacatc
    14821 ttgggctgat tggacaaaac ggagccggta aaagtacatt gattaaaatc atcactggtg
    14881 agatcttacc agatgacgga cagatccatt ggcaaaaaaa tattcacaaa ggttacctcg
    14941 atcaatatgt ggaagtcgat gaaacattaa caatcgaaga atttctaaaa actgcgtacg
    15001 caaaagaata cgaaaaggaa gaaaaaattg cttttcttta ccaaacatat agcgagacct
    15061 tcgatgattc cttgctggaa aaagctggga aactccaaac ggaattagac caaggagtgt
    15121 tttatcaaat cgacacctta gttgctgaga tgagcagcgg gttggggatc gatgtcttag
    15181 gtttagattc actgttgaaa aatctaagtg gtggacaacg ttcaaaggtg attttagcaa
    15241 aactactttt agaaaaccca gatgttctga tcttagatga accaaccaat cacttagatg
    15301 atcagcatat ccaatggctg acgcagtttc ttcaggattt tgacggtgcg tatatcgtga
    15361 tttcccatga ccaagaattt cttgatcaga tcaccaccca tatcgcagac atcgagtttg
    15421 gaaagattac taagtatact ggccatctaa aacaagcatt gaaacaaaag gaacaaaatc
    15481 gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca aatcgaaaag accgaagctt
    15541 atattcgaaa atacaaagcc ggttcccgat caacgatggc aaaaagccga caaaaacaat
    15601 tagataaaat cgaacggctg acgcctccag cttcagcaag taaagctgtc tttgattttc
    15661 cattctcgcc tattgtcacg acattagcag tggaaacaac tgatttagtc atcggatatg
    15721 atcgaccgct acttgcaccc atcaatttga ccatccgctt tggtgaaaat attgccattc
    15781 gaggcttcaa cgggattgga aaatctactt tattaaaaac actgatcggt gagattgaaa
    15841 aaataagtgg cgatttccac ttcccagata acacaaaaat caattacttt tcacaagatc
    15901 tgtactggga aaatccatta gaaacaccac tacaatattt aagcagtcaa tttccaaaag
    15961 cgactatcaa agaattacgt cgccaactag ctaaagcagg gttagttaat caattagcaa
    16021 gtgaacctct cactacatta agtggtgggg aacaaactaa ggtgaaactt gcacagttga
    16081 caatgaacca aggtaattta ctgattctcg atgaaccgac gaaccatatc gatcaagaaa
    16141 cgaaagaaag cttacaagac gggatacaga aatttccagg aacagtaatc atcgtctctc
    16201 atgaacaaga attttatcaa gatcttgtgc aacgagtcat tgagattgaa ccaaaataaa
    16261 cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa gttttgtcac aactccttct
    16321 tctgaataaa tggtggccaa ttagctgacc ttcggtatta gctcaaaaaa cggcaaaata
    16381 tgaggagcta tttttatttg gctattctta ttacttgatg cagaacagct tttcacaacc
    16441 tcttttcgtc tattttttat tttcgagatt tctctttcct ttaaaaacaa ctgtttgaaa
    16501 gaagaacgca catccttgaa aaacaagccc gctaaccgtt acacctaacc agccatactg
    16561 ctgccacatt gtcgtcccta gcaatgaacc agccgcacct ccaataaagt acataaacat
    16621 aaataca
//
LOCUS       DAZAFF010000061        16471 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000061 DAZAFF010000000
VERSION     DAZAFF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.061"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACG9BB_001892"
     CDS             complement(<1..1835)
                     /locus_tag="ACG9BB_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN2837824.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACG9BB_001893"
     CDS             complement(1832..2536)
                     /locus_tag="ACG9BB_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN2837825.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACG9BB_001894"
     CDS             complement(2548..4857)
                     /locus_tag="ACG9BB_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFN2837826.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACG9BB_001895"
     CDS             complement(5052..5516)
                     /locus_tag="ACG9BB_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837827.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACG9BB_001896"
     CDS             complement(5516..6142)
                     /locus_tag="ACG9BB_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN2837828.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACG9BB_001897"
     CDS             complement(6149..6514)
                     /locus_tag="ACG9BB_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFN2837829.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACG9BB_001898"
     CDS             complement(6504..6842)
                     /locus_tag="ACG9BB_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837830.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACG9BB_001899"
     CDS             complement(6832..7158)
                     /locus_tag="ACG9BB_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFN2837831.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACG9BB_001900"
     CDS             complement(7139..7417)
                     /locus_tag="ACG9BB_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFN2837832.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACG9BB_001901"
     CDS             complement(7419..8783)
                     /locus_tag="ACG9BB_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFN2837833.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACG9BB_001902"
     CDS             complement(8796..9365)
                     /locus_tag="ACG9BB_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFN2837834.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACG9BB_001903"
     CDS             complement(9337..10524)
                     /locus_tag="ACG9BB_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN2837835.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSSYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACG9BB_001904"
     CDS             complement(10588..12315)
                     /locus_tag="ACG9BB_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFN2837836.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACG9BB_001905"
     CDS             complement(12312..12764)
                     /locus_tag="ACG9BB_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFN2837837.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACG9BB_001906"
     CDS             complement(12876..13256)
                     /locus_tag="ACG9BB_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFN2837838.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACG9BB_001907"
     CDS             complement(13253..13639)
                     /locus_tag="ACG9BB_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFN2837839.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACG9BB_001908"
     CDS             complement(13641..13919)
                     /locus_tag="ACG9BB_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837840.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACG9BB_001909"
     CDS             13967..14695
                     /locus_tag="ACG9BB_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFN2837841.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14826..15302)
                     /locus_tag="ACG9BB_001910"
     CDS             complement(14826..15302)
                     /locus_tag="ACG9BB_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFN2837842.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15262..15618)
                     /locus_tag="ACG9BB_001911"
     CDS             complement(15262..15618)
                     /locus_tag="ACG9BB_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837843.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15596..15781)
                     /locus_tag="ACG9BB_001912"
     CDS             complement(15596..15781)
                     /locus_tag="ACG9BB_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837844.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15782..16054)
                     /locus_tag="ACG9BB_001913"
     CDS             complement(15782..16054)
                     /locus_tag="ACG9BB_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFN2837845.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16051..16251)
                     /locus_tag="ACG9BB_001914"
     CDS             complement(16051..16251)
                     /locus_tag="ACG9BB_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837846.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
BASE COUNT         4635 a         3467 c         2554 g         5815 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc tagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta cgaacattta gtgaacagtg
    14821 taattttaga ataaagagag ttgttctttg tacttctcgc tattgtaaaa atcaatttcg
    14881 ttttcttttt tcaatcggtc agcttgtctg tgttcatact catctaaaaa gttcaatgtc
    14941 tttctaattt ctgcatgtcg ctgtctgaca tacgatgagc tatacccaac agattcagca
    15001 atatcttcta agctcatttg atcaacatat ttcatcttaa caatttgatt atcgatacca
    15061 gaaaagctat ctatcagcaa taacatttct tctttttgct cgatcaacaa ttccaattca
    15121 ttttctatct tttggatatt ctcttctaaa gaagaagttc tcgaattctt ttcgatgcgg
    15181 acgtttgcta aatcaccaca gacccatcga ttcaattcaa gcttactttt attaagattc
    15241 cactttaagt aaagaatctg ctcatctagt tcttggtaat cttttaacca atgaaatctc
    15301 acaaacgcca ccccttatgg tagaatagta ttgcgctgct atctcgaaag gggtagcttt
    15361 tttatttttt cttagccgtt ccattctgga ttgccttttg ttccaaacgt cgttttttct
    15421 tcttaatctt tgattttgtt tttcccatac aattctcctt tccgaaaaca cattttgttc
    15481 gatttttaat actaaataaa agttggccag aatttttcgt tcgtctttac tcatcttttt
    15541 ttgaataaga ttactagcat aaaaatagtt agtaccacga aaatgaacag gtaaatcaat
    15601 tacgttcacc ttcttcatgc cagttatcag ccactagaat ctttgcgtta gtgccgccac
    15661 cgttttcttt tttgtatttt tcaaatgctt tataagcagc tgtttttgat ttatagattt
    15721 ttccggaacc gactgggaac atccatgtgc cctctgtgat agctaagatg tatactttca
    15781 tttaattcac ccctttaatc cccaacacta catatccgga cttctgcatg taatcagtaa
    15841 tataagtgat ctctgctttg tatgagtcac cagtgtaact aaatgttggt ctcgttagtc
    15901 caggtaattc atgtttaatg tattttttat attcacgtag tattaactga tcaccaactt
    15961 tgaagtcacg atcattcttg cggatctcga attgtttgcg tccgctcgtc actgcttcaa
    16021 aatattctgg tagtattttt agttcgtgga tcattctgtt tcctccttca aaattcgctc
    16081 atcatcaaaa agtgaatctc cacaaaatcc ttctcccaaa tatttatcgt tcatgtataa
    16141 gtcaaccaaa tatttgtgat ctgcttctcg atattcatta atattaacga tctttatttt
    16201 gtaaacatta ccttcgtcgc tcactatctt gaattgttct ctgactttca ccaccgttcc
    16261 tcctcttttt gaaaaaattt tagtgttttt gtaagacccc ctaaatcagc ttttttattt
    16321 gatataatac ttgtatcgat taatcaaatc gaaattaaat ttaaaaaggt gatttatcat
    16381 gaatatcaac aatttagtaa aactcgttga acaacgtttt aaagaatcag gattagaaca
    16441 attaaaaaaa gataattctc cagaattatt t
//
LOCUS       DAZAFF010000062        16315 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000062 DAZAFF010000000
VERSION     DAZAFF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.062"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..1495
                     /locus_tag="ACG9BB_001915"
     CDS             68..1495
                     /locus_tag="ACG9BB_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2837847.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1587..1952)
                     /locus_tag="ACG9BB_001916"
     CDS             complement(1587..1952)
                     /locus_tag="ACG9BB_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFN2837848.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1949..2314)
                     /locus_tag="ACG9BB_001917"
     CDS             complement(1949..2314)
                     /locus_tag="ACG9BB_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFN2837849.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2506..2754)
                     /locus_tag="ACG9BB_001918"
     CDS             complement(2506..2754)
                     /locus_tag="ACG9BB_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837850.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2753..2881
                     /locus_tag="ACG9BB_001919"
     CDS             2753..2881
                     /locus_tag="ACG9BB_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFN2837851.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2938..4632
                     /locus_tag="ACG9BB_001920"
     CDS             2938..4632
                     /locus_tag="ACG9BB_001920"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFN2837852.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4699..4905
                     /locus_tag="ACG9BB_001921"
     CDS             4699..4905
                     /locus_tag="ACG9BB_001921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837853.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5283..6416
                     /locus_tag="ACG9BB_001922"
     CDS             5283..6416
                     /locus_tag="ACG9BB_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFN2837854.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6447..6767
                     /locus_tag="ACG9BB_001923"
     CDS             6447..6767
                     /locus_tag="ACG9BB_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFN2837855.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6796..7260)
                     /locus_tag="ACG9BB_001924"
     CDS             complement(6796..7260)
                     /locus_tag="ACG9BB_001924"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2837856.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7392..8645)
                     /locus_tag="ACG9BB_001925"
     CDS             complement(7392..8645)
                     /locus_tag="ACG9BB_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN2837857.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8674..8991)
                     /locus_tag="ACG9BB_001926"
     CDS             complement(8674..8991)
                     /locus_tag="ACG9BB_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN2837858.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9017..9337)
                     /locus_tag="ACG9BB_001927"
     CDS             complement(9017..9337)
                     /locus_tag="ACG9BB_001927"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2837859.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9801..11216)
                     /locus_tag="ACG9BB_001928"
     CDS             complement(9801..11216)
                     /locus_tag="ACG9BB_001928"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN2837860.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11233..12549)
                     /locus_tag="ACG9BB_001929"
     CDS             complement(11233..12549)
                     /locus_tag="ACG9BB_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN2837861.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12699..13397)
                     /locus_tag="ACG9BB_001930"
     CDS             complement(12699..13397)
                     /locus_tag="ACG9BB_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFN2837862.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13686..14675)
                     /locus_tag="ACG9BB_001931"
     CDS             complement(13686..14675)
                     /locus_tag="ACG9BB_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFN2837863.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14755..15588)
                     /gene="msrA"
                     /locus_tag="ACG9BB_001932"
     CDS             complement(14755..15588)
                     /gene="msrA"
                     /locus_tag="ACG9BB_001932"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFN2837864.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15763..>16315)
                     /locus_tag="ACG9BB_001933"
     CDS             complement(15763..>16315)
                     /locus_tag="ACG9BB_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837865.1"
                     /translation="RRYNKTRELFYLFMTVLLLITLTVSSKKNFNQKIISQPGHIVVN
                     KETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRV
                     TNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTT
                     IISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         5085 a         3284 c         2767 g         5179 t
ORIGIN      
        1 ttcaaaaaga aaacatgtca taatatttcc catattgtgc aaaaaaatac aaggcggagg
       61 ctctactatg attgaagaat atattgagaa aaatatttta agacagctgt ttttatgtgg
      121 gcaattctat gtaaataaag aagtcaatct agaaaagctc tccaatctcc tgcatgtttg
      181 caaaaccact ttactcaatg atatcaataa cataaaaaaa gaatttgaag aacagatcgc
      241 ttatacacat cgggaaaagg accgttacac tttatatttt tcagaacata tacctcgatg
      301 caagatcatg cagcaattgt ctcaaaattc gttgttttta aaaacttgtc tgctttattt
      361 ggaagaagat gaacctgatt atcttcaatt aactgaatgt gaattcatct ctgtcagcaa
      421 agcttattca ttaaaaaaac aagtattagc ttattttaat gactgcggga tcgaaatcga
      481 ccgctactcc cctcgattca ctgaaatgga aagacgtctg ctgcttctta atgtgtcgta
      541 tcgtttaggc ggttttaatt cttgggagct acctgaatcg ttttttgaac gtgccgatcg
      601 tttcattgaa tccgtaacgg aaaatagtgg cagattttat gataaagaaa ataaagaaat
      661 cttatcgatt ggttttgcta tcagtttcct acgccagcaa gtgtgtgctg tgacaataga
      721 ttcaaaattt atcgaagaaa tcaaaaaacg tcctatttac aattatgtcg aatcagcatg
      781 ggaaaacact gatttccaaa cctattataa aaaagaagaa ttcgccttta ttttgacact
      841 attcaatctt tgtaattatg gtttccattc ttatcagttg attgcggaag actttcaaca
      901 gctacaccaa gtgtttattg acaatactcc cgaaataaaa gaactagttg ctacctttga
      961 atctcacttc aatcaagaat tattcggaaa ccaaccgttc gaacgtgcgc tgattcatct
     1021 gatgcgttcg gcttgggata actatcaatt attcatgcca gaaaaatttt atcttttaaa
     1081 tgaggaacag acaaatttat ataaagaagt ccagacaatt ttttccagct ggtccagcca
     1141 gttaccttat gatctacggt tgaatcctaa ttgcatgcgg gcttttgtga tcgaattgag
     1201 cggaattctc cgtctgacaa aagaacatct gactatctat atcgtcacaa attcggatgt
     1261 tcattatctg atttatcggg aagccttgga agctgtaact actttcgatt ttcaagttgc
     1321 accaacaatt tattcaagta tatctgatat aaagaagtat gcgcagcagt cctcaaaccg
     1381 ggtattatgt gaacgtacat tatatacacc agatgcggtc caatatgaaa atattattcc
     1441 tatttcgatc aatacgatcg atcgagctat catttcagct gtgcaaaaca aataaggggc
     1501 tgtgacaaaa atcatgtcac agcctcttat ttgttgaaga tgcccatttc tgtaacaatc
     1561 tcttgataca cgtcaggctc acaacctcac ttattttgga catctacggg tccgatctct
     1621 ttatttaaaa aataagaaat gatggttcgg ataactacca acgccgctag ttggaaaata
     1681 tcctgcaagg taggtttgat aatcgattcg atgatcccag ctgaaacaag aatttccaaa
     1741 cttagaagta tatagcctcc caacatcttt ttaatagcat tgttctcgct gactcggtcg
     1801 ctttgcactt ttgtatggac aaataaatat ttcaaaaaat ccttgattga tagacaaaca
     1861 ccccaaacca ataaacagat agcaaagata tcaagtccca gtacaagttt ttcgaaatat
     1921 ggaagaagaa actgcatcaa ctctgtcgtc atcctacctc tcctcttctt tgtttttctt
     1981 tgtcttctat ttcctgattc aagaaataag aaatgactgt tcgaatgata ataataagtg
     2041 caagcttcaa aatatctgtc caagtcggct tgataactga ctcgatgata tcagcaacga
     2101 ctagtacttc caagcttaac aaaatataac ttgctagcag ttttcttgta tctgcattac
     2161 gagtattacg ttctgcatag tttttcttga catgagacca atcaaaaagt ttgaatagaa
     2221 caagaacgat cccgacaatt aagataccga tagaacaaat atttaaaata gcaatgatcc
     2281 attctagaaa ttcaattaaa tttgagcgaa acatatacgc tacctacttt cttttaattt
     2341 gttcctattc ttttattgta aaagaaagtt accttgttcg tctatttaaa agttgttcta
     2401 atgataacgg ttcgcttaaa tttttctttt tcactctccc atttttctcc ctccatccat
     2461 aaatcgtgga agtatccaaa acaacgtagc aagttaaaat aaggcttagt ccgtcatcag
     2521 cttttcaaag aagcactgtc tcggtgcttt ttttgtaccc aaatttctaa aaatagaacc
     2581 atacacctcc tcaaatcttt tcttcttacc taccctattt ttatttacag gaaaatcaga
     2641 cacttcttct tttttgtcaa aatttccttt ttcttctcgt cctatcattt ggcattctaa
     2701 taaaaacgaa ctatccttgg atatagaaag agaaaacaag gaggcgtttt tcatgagtaa
     2761 aaaattaatc aaagtcggca tcggtttagg tttgcttgcc cttggcgctg catatctagg
     2821 aaaaaagaca ggactattcg aagatgacag tcatttatat gatgaattcg aatcaatcta
     2881 gcatcctatt tagttgaatc actacggaca tacttggaag acattggagg aatcacaatg
     2941 agaaaaagta aagcaataat gattggtgcc ggtattgcca atatggcagc ggctgtttat
     3001 ttgattcaag aaggcaaatg gaaaggggat cagatcacct tttattcatt ggatgatcac
     3061 ggttcaaatg atggtgcacc gaccgacact gtagtggatg aatattggaa taaaaaccat
     3121 ccattagaaa accaaaaagg ctatattgct cgaggcggtc gcatgcttaa ttaccgtacc
     3181 tatgtagatt tgatggatct gttaagtcga ataccttctg ttactgagcc agggatgact
     3241 gctgaagaag atacgagaga ttttgatgca aaacaccgaa cctttgacaa agcacgtttg
     3301 atggaaggtg gcaaaggaat cattgatggc ggaaaactgg gattcaataa taaagaccgt
     3361 attcttctga ctaaactgat tgctatgcct gattcagaag aagaaaaact agacaatatc
     3421 actatcgaag aatatttcaa agatgatcca cacttttttg aaacaaactt ctggtttatg
     3481 tgggaaacaa catttgcttt cagaacacgt agttctgctc aagaattgcg tcgctatatg
     3541 catcaaatga tttatgaatt cactcaaatc gaacatctag tgggcgtcaa tcgaacacgt
     3601 tacaatcaat acgaaagtat tatgcttcct ttgatcgagt acttgaaaga acaaggatgc
     3661 aaaatcattt tgaaccgccg tgtcactgac tgggaattca aagaaacacc gatgcaagat
     3721 gaaatcactg tgactggttt gaaaattacc aatacactga ctcaagaaga agaatacgtt
     3781 gtggtcgatg aagatacagc agttattttc actaatgggt ccattacaga ttcagctact
     3841 ttaggagatt acgaaacacc agctgtagaa aatatggatt atggtgcagc ttctagtctc
     3901 tggaaaaaag ccagtgaacg tttctacaac ttgggaaatc ctgacaaatt ctttgctgat
     3961 cgagatgcaa gcgaatgggt cagctttacc ctgacgacga aaaaccattt gttgttgaat
     4021 gaaatcaccc gtatcacgac tcaagaacca ggaaatgcct tgaattcatt tatttcaacc
     4081 acaccgatca cgccgttagg ccaaaaagat gtcaatatgt ctattgtcgt gcatcatcaa
     4141 ccgcatttta cgtctcagaa accaaatgaa acagttattt ggggttattt cctttatcca
     4201 agacgacgtg gcgaatttgt ggataaacag tatattaaaa tgaatggaaa agaaatgcta
     4261 gaagaactga ttggacaact ttcaaaagta gatcctggac cagtgaatat tcgtgaaaaa
     4321 gaaacagaaa ttttcgacag cgtaataaat aatatccctg tctatatgcc ttatgcttcc
     4381 gcgttgttca ataatcgtgc caaaagcgat cgaccaaaag tcattcctaa acattcgacc
     4441 aatctggcct ttacaggaga atttgtcgag cagccttatc agatgatctt tactgaacaa
     4501 agtgctgtac gttctggaga aatcgctgct ttccattttg cgggtattcc tatgtcccgt
     4561 cttgtcaaaa caccgcgtta cgacaaagat attcctactc tagctcgtgc cgcaaaaaaa
     4621 atgtttgaat aatcaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacaacc
     4681 aggctaaaac agcttcttat gtttttgatt tggttggact tattaccgca gatgcaagct
     4741 tttgaacacc gtttaccaag aggttatgaa aaagctgccc tgcatcaagt aataagagca
     4801 gccaagctaa aacagcttct tatgtttttg acttggttag acttattacc gcagatgcaa
     4861 gcttttgaac accgtttacc aagaggttat gaaaaaggcg tttagtttcg agtatgaaga
     4921 aaaattttgt gaaaatggtt cctcacgttt ttgcataaat ttgacttaat accgagaaac
     4981 tgatcccttc tgtcataacc tctttttatt tatgtctgtt tctttttgaa aacaactatt
     5041 ttttcaacgt ttactctctt gccaatccaa catcatctaa cggatacaac tatttcaatg
     5101 attaccatta cactaaaaaa tcggaaacac acctctccga acacaaatac ataagactat
     5161 agaatgtttc atttaagaaa aaaagcgttt cattatcaaa aaggtaagat ctcctttaaa
     5221 atgaaattat ctgtagtact aataatgcag acaattttat gaatctgaaa ggagcatctc
     5281 caatgaaagc agtcacttgg caaggaaaac agaaaatgga aatcagaaac gtagatgatc
     5341 caaaaatatt agaaccaaca gatgcaatca tccgtatcac agcaacagcc atttgcggct
     5401 ccgatctgca tctttaccat catggtgaag cagttttgga aaaagggtat gttgtaggac
     5461 acgaaccaat gggaatcgtt gaagaagtgg gatctcaagt tcaaacattg aaaaaagggc
     5521 aacgtgtggt tatccctttt aatattagtt gtggtcattg tcatttctgc ctgaatcaca
     5581 tggaaagcca atgtgatgaa tcaaatccag atcagttata tggcgggctt ttcggatttg
     5641 gcaagttgaa cggtaatcat tggggtggtc aagcggaata tctaagagtt ccttatgcag
     5701 actctacttc atttgttgta ccagataatg atcttcctga tgaaaaagtt ttatttctat
     5761 cagatatttt gcccacagct tattggagcg ttgaaaacgc aggagtaaaa aaaggcgata
     5821 cagttgtgat tctaggttct ggtccagtcg gtctattcgc tcaaaaattc gctgttatgg
     5881 ctggagcaga gcgcgtaatc gcagtagatc cagtacagca ccgtctagat aaagcggcta
     5941 attacaatgg ggttgaaact tatttcttag aagacacggc acaagctgga acagaattgt
     6001 atgaactggc taaaggtggc gcagatgtca ttatcgattg tgtaggaatg gacggattag
     6061 agcctgtcaa agaaaaagcg aaaaatttag taagcttaca aagcggcaca atctctccat
     6121 tacagatggc atcgcaagct gtcaaaaaat ttggaacagt ccaagtaact ggtgtttata
     6181 tgactccagc ttcttcttac ccgctgcaag aattttttat gcgggatatc gctgtcaagc
     6241 atggacaggc accaattatc catctaatgc caaaaattta cgcaatgatt gcagaaggac
     6301 tattcgatcc tacacaaatc attactcatt cgctgccact atcagaagca gcacaagcat
     6361 atgagatctt tgacaaaaaa gaagataaaa atataaaagt tgttttgaaa ccttgaattc
     6421 agtaaaaaat acaggtgatt ttctttatgc ccaaaatcaa acgaaatacc catgagatcg
     6481 aaataggaaa agaagaagat atcgaaatac gaggacgtcg cttccgcttc tatttccaaa
     6541 atcgttacac attgatctca ctggctgtcg atttattaac cgggattttc tatatttttg
     6601 gaagccttgc ttccttaact aaaatccccg atcgatatgg cacctatttt tatttggctg
     6661 gtgctatttt cttgacgatt cgtcctattc tcagaatcgt aaggaacatt tttatttacg
     6721 acgaaaaaaa agcaatgaag agaacaagca aaaaaaaaga ggcatgagaa tttttcaaat
     6781 ctcatgcctc ttttttcacc ctatcgtatc actttgatac agacgagcgg tcgtcccact
     6841 ttcttccttg accattgtca aaaatgtcca ctcgtatcgt tcataaaacg tggtatgatc
     6901 tgtaattaag taaagttttt cgtacccaag ttgatgcatt tcttttctga tttccgctaa
     6961 tatccgcttg gctatcccct gcttacgctt ttccggttca acaaacaggg cacaaagatt
     7021 tggtgtcaga tcagggcggt tatggaagtc gttttcaatg acgcccgctc ctccgacgat
     7081 ggtttctttt tcatctaaga ccacatacca ttgaggaata ctttccttat tagcgattcc
     7141 agtacgaata ctttcctcat acacaacagc aggaactccc cattttgatg aaaaccaatt
     7201 cgcgctttgt tttactaatt ctgggtgttc tcttaatttg atccagttat atgtaaccaa
     7261 tgtttaaacc tccgactaaa ataaaacgtt tacaaaaatt atacaagtct ttatgaaaat
     7321 tagcaatgac aacaatagtg atgtaaaaaa aaaaacggaa aacaatcact tgttctccgt
     7381 tttttgatta atcaagcatc taaagcttct tttcttcttt cttggatcgt agctaccttg
     7441 acgaacggta gatagatcaa aatagaaaga accaaattga cagcggctag tacaccacct
     7501 gcaatacttt ttgttgccaa tacaccgcca ataattggtg gagttaccca tggaggcata
     7561 acggtagctg ctggcactaa acctgtagaa gtcgcaaagt aagcaacact aactaagacc
     7621 attggtgtca aaataaatgg aatgaacatg atcgggttca acacgatagg taacccaaac
     7681 atcgttggtt cattaatatt gaagacacct ggcgcaatcg acaagtttgt tacaaccata
     7741 tatggtttgt ttttacgtcc tactaaataa atagctaaca gtaaacctaa agttgcacct
     7801 gttcctccta agttgacaaa gctgtcaaag aaaggtttgt tcacgataaa cggtgcgcta
     7861 tgtccagctg cgattgcatt aacgtttgct tcaatggctg gcgcattgat tgtttgcatc
     7921 aaaggatcga ccatattcgc tccgtgtaaa ccaaagaacc ataagaatgg tgtgataaat
     7981 gcaagcagta aggcagatgg ataagagttt gcaagtccca taaatggttc ttggaccact
     8041 gtatagaaag aacctacgat atcatcaaca ccgaatgcag caaaaattgc agcaactaaa
     8101 gcaaacgcag agatagtgat cattgctggt agtaatgaag caaatgcttt tgcaactgcc
     8161 ggtggcacac catcaggcat tttgatgatc aatttttcat ttccgcttaa acgtacaaag
     8221 atttctgttg aaataatccc aacaataata gcaataaaca aaccgtttga gctcatacct
     8281 aatgctccgc ctaaagcgaa gaaagagcct aaagaaatca cacctgcgga aacaccgtct
     8341 tttcggtagc cgttagctaa atgataagca agcaagaaag caatcaagac agaaagaatg
     8401 gcaaatgtac cgttccaagc agcagccccg aatcctttcc atgattcacc accaaaaatt
     8461 gagttcatca attcttggaa cccgggaatc ggtaagttat tgatcattac tgctaatgct
     8521 cctaagatca ttaacggcat actcaccata aatgagtccc gaatcgcaac taaatgccgt
     8581 tgtgcaccaa tttttgatgc tacaggtata aaatgtttct ccataaatgc aacaaattta
     8641 tccataaatc ttccctcttt tctttactgt agattacgcg ttcaatcgac gatacagatc
     8701 aatgatctct tttgctaaat ctgtaaaagc tatagatgtc atcagatgat cttgactgtg
     8761 gaccgtcaaa agcgtgactt caacgtgatt tccttgtgct tcttgtgtca gcatctctgt
     8821 ttgagagtga tgtgcttgga ctaaagattc ctctgcatct ttgatttttt catctgctaa
     8881 agtgaaatca ccttttttcg cagcagcaat cgcttccatt gcatcgcttt ttgcatttcc
     8941 gccaaacatg atcagaccca tgatggcttc tagatttttt tgatcttcca tttattgtat
     9001 cctccgataa tttttattat ttgtttatta gtttttctgc ttggtcgagt actttttcac
     9061 cattcatcat gccatagtca gccatattga ttacatctaa aggaatgcct ttaggtgcta
     9121 gcttttcagc aaattgattt tgcataaagc gtacttgcgg tcctaaaagt aaaacgtcta
     9181 catttttaga ttctagttga ttatccgcat ctgaagcaga tacagcaaag atgtctgctt
     9241 ccattccttt tgcttctgct gccttttgca tttttgtgac taggagactt gtactcattc
     9301 cagctgaaca tacaagcatg attgttttct tttccatttt aaacacgttc ctttctatac
     9361 ttacacttgt atataagcaa gtagcgtgcc aactttaaaa gcgctttaaa aagttgatat
     9421 catcacattt aaggtctata ccaagttgca tacacactaa aaaagaaagc gcacactaaa
     9481 cacacaaatt aatttttatt accttttttc ttttaacgtg cacaaaaaaa gttgtgacac
     9541 aaaacttgtc acaacttttt tcaaataaac ggtattcaaa agcttgcatc tgcggtaata
     9601 agtccaacca agttaaaaac ataagatgtt gtttttactt tgctattctt attacttgat
     9661 gcaggacagc tttttcacaa cctctcaata aacggtgttc aatcagctga ccctgggtat
     9721 tagctcaaaa cgctggattc acaacctctt ttatttcaaa taaccagtaa atacaatgaa
     9781 catctacaaa aaacttctat ttatttatag atttcgtcag gcaaatccaa ttctctctta
     9841 tcaatcaaag tctgatacca cgaagcagat tttttcaatg ttctgcttcg ttcatcgttc
     9901 aattcgattc gaactaatcc atatcgattc ttaaatgcat tcattggtga cacacagtca
     9961 gtgaatgccc ataacatata gccttcgcaa tttgcgcctt cttctgttgc tttgatcaag
    10021 taatgcaaat gctgtccgat aaattcgata cggtaatcat cttgcacgat tcctttctca
    10081 tccatatact gttcttcata ttctcttccc ataccgtttt ctgttatcag ccaagggata
    10141 ttatgataat tttcttttag atacatggct atatcataca tgattttcgg atagatttcc
    10201 catcccctgg aattattcat tttcttccca ggtaaatcaa agacttcata atatcgttca
    10261 ggatggaaag gtgtctcttc attccattga taacgagggg cgcgaacgcg tttaggataa
    10321 tattgattga ttccaaggaa atctactgta tgatgctgaa tcgtttctaa atcctctgtt
    10381 gcaggatcga aataaatcga ttgtttttga cacaactcca ggaactcttc tgtgtagtat
    10441 ccttttacca tcggatcaaa aaatactcga ttataaaaca aatcatacat ataagcagct
    10501 ttttggtctt ctggagaatt ggatcgagca tagaccattt caggatttaa aatacagcca
    10561 atccttccac cataatttcc gctgcggtaa atctctactg ctttagccgt tgctaaaact
    10621 ttatgataat tccacatcat ccactttttt gtgttttgtt catgcggcca gcgaacagca
    10681 tccaaatagc aacgcgtttg gggaacgatt ggttcgttga atgtaaacca gttttttacc
    10741 ctatcaccgt aacgatcaaa ggcaatttga gcatacttag cataaaaatc aactactttt
    10801 cttgaagacc agccatcata tttttccaaa agaaaagctg ggacttcata atgttccaaa
    10861 caaatcatcg gttctacgtc tgcttcgatc aaagagtcga tcatttcact tatatgctga
    10921 gcatattctt catctactat acagttctca taatcagtaa aaaaacgagc ccaattgata
    10981 gaagtccgat aatgtgtcag atggatggat ttcatcagat ccacatcctg ctggtattgt
    11041 tccataaaat tagttgctac ggctgggcca tacccattat gccaaacaaa atgttcattt
    11101 ttataccagc tatctataaa cgaatcctgt ccttcttttt ttcctttcca accttccgtt
    11161 tgccaagcag aagatgcagc accaagaata aaattttttg gaatagtaat tttcatgatt
    11221 tcctcctgtt ttttagatcc catcattttc cacacgattt gctactttta caaatggcag
    11281 atagatcaaa atagaaacaa taatacaaac aagttgcgta ataactgcac cgatatttcc
    11341 tgctgttgcc agataagcat tgacgatcgg cggcattggc caagggacca ttacaactgc
    11401 tttcgccgca aatcctaaag cggttgcaaa ataaccaatc gttccagtga taagaggcgt
    11461 taagataaac ggaatagcta aaattggatt cagcatgatc ggcataccaa aaataactgg
    11521 ttcattgatg ttgaagatcc caggagcaag ggataacttg gtgatttgtt tcatatcttc
    11581 ccgttttcca actaacaata ctgcaatcag taaaccgatc gtgactccag acccgcccat
    11641 actcatatac atatcccaaa atggcatagt aataatgttc ggtacttctt ttccagcttc
    11701 aaaagcttcc gtatttacag caattgccgc taaaagcaac ggttcccgta tcggcttcac
    11761 catttgattt ccatggatac caatcaccca aaagacttgc gcaataaaca tcaatagcaa
    11821 aatacccggt aacccttgta cgacaccttc taatgggcgt tgtacgacat tgtaaatgat
    11881 atcataaagg tacattcctg taagttcttt gatgataaat cccgcagccg cgatacccga
    11941 tacagtcaga atcgtcggga ataaagcaga aaaactagca gaaacattcg ttggcacggt
    12001 atcaggcatt ctcagctgta gtttcttctg tttcctcaac cagcaataaa gttcaataga
    12061 aaggatggag accaacattc ccaaaaataa ccctttcgta tctgtatatt gtttagccaa
    12121 tacgttttct actataacag tctgattttc attcactaat tcataaacag ttggattaac
    12181 agataaataa gacatcactg cgatgatacc agggaataca ccatccactt tattcagctt
    12241 ggctaattcg atccctatca gaaaaacagc atagatggtc aagaaactta gtgtgacata
    12301 gctgatggca gatgcgatag gctttaattc ttctaaaaaa cgcaagaaac ttatctgtgc
    12361 taagccattt tccgagtcaa agaccattgc tgaaaacaat gtgccaaaag cacccgtaat
    12421 aattaccgga atcaatgcag caaatgcgtt ttttatcacc atgatatacc ttaaattatt
    12481 gattctcgtt gccacgttcc ctaaaacatt ggctaattta tccataaaac tcattccaga
    12541 tcccctcatt cctgcttctt ctccttgacg tcatctcttt ttgtctatta gtcagcgctt
    12601 acatttcgat gaacagaaac tatttactcc tcttttttac caaatataaa acaaaaattc
    12661 atctgatgta tttatcataa aatacatcag atgaactgtc aattagtttc tttttctcct
    12721 attattttct gtaattctct ccgatttatt gccatatgga cagtcattgc atcaaacgca
    12781 gcaagtgcat tccgcctttc gatggcctga acgatctctg tatgaatatc gatcgtctcg
    12841 attttcagat gctggatatc ataattttta ttaaacaaag caattgattc attaatgatt
    12901 gggataatat gcagcaaggc tacgtttccg ctagcttctg caatcatcgt atgaaaatcg
    12961 atgtctaatt gaacatgttc ttgtgtgcct tctcgaaaag aagtttcaat cttttgcgtt
    13021 aattttttca tttggacgat ttgattcttc gttgcatatt ccgctgccaa acctgccatc
    13081 ttaggctcca ataaatatct cagttcaaat aagtctcgta tcatccgatc ctgatcttca
    13141 atcaaagaaa aaccgagtgg gtcattggca atccctcgct tatcactgac aaatgtcccc
    13201 gctccttgcc gaacttcaag gatattcctg gaagctagag cccgcacagc ttctcgcacc
    13261 gtgcttcgcc ccacctctaa ataatcggac aattcatatt cattcggcag tttatctcct
    13321 acttgcaact gcatttcctt taaatatcta aggatcttgt cagtagttac ttctactagg
    13381 gttttatttt cttccaaacc acatacctcc ttttcatctg atgtattttt tcgtaattat
    13441 tataacacgt ttgaatcatc tgatgatttt ttgtatacaa aattgattaa tccgttcata
    13501 gacatacaaa cgaagaagat atttaccata aaaaagacta ggataaaatt cactcctagc
    13561 cttcgatcat ttttattttg aacgacaaaa aatatgatgt tcaataacag acgatcttca
    13621 ttcgattctt gatgtcatct tttgtttttt ttaatatatg gataaaaagt ttgattacat
    13681 cgtttctatt ctagaatttg gtcgattctc aattcttctt ctggtgagaa atctaaatta
    13741 tccatcattc gaacactttc ttgtaactga cttaaccgac ttgctccgac cagtacactt
    13801 gtaacagatt tttgtcgcaa attccatgca agggccattt gcgctaatga ttgtcctcgt
    13861 gatacggcga ttgtttgcag tgctttgatt ttttccagag ttgatccaac ttgttcttct
    13921 gaaaggaatg gtatttcctt acgatgtgct ctagaatctt caggaatacc atgtagataa
    13981 cgatttgtca gcaacccttg agcaagcgga ctgaacgtga ttgcccctag cccttcttct
    14041 tctaatactg gagatagatc gtcttctagt agtgcacgag aaaacatatt gtagcgcatc
    14101 tgatgaataa tgaacggtgc ttcctttctt tttaaaattt cagtcatttt ttttgtatct
    14161 tctccattat agttggatat accaatatat aatgcttttc cttgtcttac aagttgcatc
    14221 aaagcttctg ctgtttcttc caaaggtgta tcagggtctg ggcgatgatg atagaagatg
    14281 tcaacataat ctagccccat tctttggaga ctttggtcac aacttgaaat gatggattta
    14341 cgagagcccc attccccata tggcccttcc cacatatgat aacctgcttt gctagaaata
    14401 atcagttcat cacggtaggc ttgaaaatct tctctaaaaa tacgcccaaa gtttttctct
    14461 gcagctccag cagggcctcc ataattattc gcaaggtcga aatgggtaat tcccatgtcg
    14521 aatgcgcctc ttaaaatctc tctttgatta tggataggat cataatcacc aaaattttgc
    14581 cataatccga gagataaaat cggtaatttc aaacctgaat gaccggattt acgatattcc
    14641 attttttcat aacgtgcact atctgcaata tacaattttt ctcctcctca tcaaatcttt
    14701 ttaaaacgct ttcttttttc tatttttagt ttactacatt ttaaagaaaa ggagttaatc
    14761 aaaatgattt tttcgtatat ttttttgcca tttgtttttt actcgttgat agatcagaaa
    14821 ttgattacgt gcttgctgga tttttttata acgtttcggt tgttttttgt aaaattgctg
    14881 atgatagttt tctgctggcc aaaacgtacc ggctggttcg atttttgtga cgattggttg
    14941 gtggaaagtt cctgaactgt ctagtttctg ttttgattgt tccgctaatt ctttttgtcg
    15001 ttcgacttga taaaaaatca ctggacgata ttgcttccct ctatcttgaa attgtccaaa
    15061 tgtatctgtg ggatcagtaa tttgccaata gattgtcagc aactcgctat aacttatttc
    15121 tcttgtgtca taaatgatct ccacagcttc cacatgacct gtgtaaccac cacttacttg
    15181 atcataattt ggtttttcaa catgtccgcc cgtgtatcct gacaaaacag ataggatccc
    15241 ttttttcgat tcaaacggct cgaccataca ccagaaacat ccccctgcaa atactgcacg
    15301 ttcttgatag tcagcatctt gcatggcatg cttggaaaag tcatattttt gctccttatc
    15361 aaatccttcc gtaatcaata aataaaagtc cattacatca ggcgtcagat tcattcggat
    15421 agctaatgga cgtagtgttg cttctagttt tgataactgt tctttaacgg atatattttc
    15481 tcttatgtca ttctttgtct gaatcaatac ttttcgttcc caatctcgtg tattcggatt
    15541 caaaatcaga ttataaagtg tttgaatgaa tatttgttcc tcatccatag attctcctcc
    15601 tttccgaaga agacaagcta gtgactttag ctgtaattta tcataaagtg aggaaataag
    15661 cgattacttc atccccctac agatttttcg tataaaaaag agagaatcct tatcgacaga
    15721 ttctcctttt taatcaattt attttataaa tgaaaagtta atttaccttt tatggaaaac
    15781 tattttaaaa aataaacata aatatgcagt taatgtaaca agataagaaa taatagtcgt
    15841 tactgtataa cttgtgctta ctttaatatc agcaactcct ttgactccgc ttatttgtac
    15901 caatccatta gcattcacct gctcctttac aggttgatga tttatttcta cattcgttcc
    15961 taaataagca tatacaggaa gtgtgattac ttggctcttc tcattagtta ctctatatat
    16021 gatagaacta ccagttgttt tataaggaag gtttacccat tgactgccaa tctttgcttc
    16081 gtgttttatt atatgctcct tattctccaa tgaagcgata ggagaataat catataaaaa
    16141 ataatccatt cctttagtgg tatacgcctc tactgtttct ttgtttacaa caatatgtcc
    16201 cggctgagaa attatttttt gattgaaatt ttttttacta gaaacagtta aagtgattaa
    16261 taacaaaaca gtcataaata aataaaatag ttctcttgtc ttattgtatc ttcta
//
LOCUS       DAZAFF010000063        16305 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000063 DAZAFF010000000
VERSION     DAZAFF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.063"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..251
                     /locus_tag="ACG9BB_001934"
     CDS             <1..251
                     /locus_tag="ACG9BB_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837866.1"
                     /translation="EGELKSLVYGETTEILLCLAMQEEHLDENLPLSAHIMRRIAVDA
                     YEQAKNEEENQPSNHTFVINNKEDLADVMTRILKGEFQ"
     gene            484..1425
                     /locus_tag="ACG9BB_001935"
     CDS             484..1425
                     /locus_tag="ACG9BB_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFN2837867.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            1427..2317
                     /locus_tag="ACG9BB_001936"
     CDS             1427..2317
                     /locus_tag="ACG9BB_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFN2837868.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            2360..3160
                     /locus_tag="ACG9BB_001937"
     CDS             2360..3160
                     /locus_tag="ACG9BB_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFN2837869.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            3175..4026
                     /locus_tag="ACG9BB_001938"
     CDS             3175..4026
                     /locus_tag="ACG9BB_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN2837870.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            4065..4226
                     /locus_tag="ACG9BB_001939"
     CDS             4065..4226
                     /locus_tag="ACG9BB_001939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837871.1"
                     /translation="MLQIIGDRDMDELSQQESRMVRKISKLIIYRDTERFLTVLGNVY
                     ENPELLEVE"
     gene            4227..4688
                     /locus_tag="ACG9BB_001940"
     CDS             4227..4688
                     /locus_tag="ACG9BB_001940"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN2837872.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            4714..5166
                     /locus_tag="ACG9BB_001941"
     CDS             4714..5166
                     /locus_tag="ACG9BB_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837873.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            5272..5466
                     /locus_tag="ACG9BB_001942"
     CDS             5272..5466
                     /locus_tag="ACG9BB_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837874.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            5497..5883
                     /locus_tag="ACG9BB_001943"
     CDS             5497..5883
                     /locus_tag="ACG9BB_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837875.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            5880..6083
                     /locus_tag="ACG9BB_001944"
     CDS             5880..6083
                     /locus_tag="ACG9BB_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFN2837876.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            6080..6376
                     /locus_tag="ACG9BB_001945"
     CDS             6080..6376
                     /locus_tag="ACG9BB_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837877.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            6452..6868
                     /locus_tag="ACG9BB_001946"
     CDS             6452..6868
                     /locus_tag="ACG9BB_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFN2837878.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            7129..7266
                     /locus_tag="ACG9BB_001947"
     CDS             7129..7266
                     /locus_tag="ACG9BB_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837879.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            7676..7906
                     /locus_tag="ACG9BB_001948"
     CDS             7676..7906
                     /locus_tag="ACG9BB_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837880.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            8065..8898
                     /gene="terS"
                     /locus_tag="ACG9BB_001949"
     CDS             8065..8898
                     /gene="terS"
                     /locus_tag="ACG9BB_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFN2837881.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            8891..10306
                     /locus_tag="ACG9BB_001950"
     CDS             8891..10306
                     /locus_tag="ACG9BB_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFN2837882.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            10318..11847
                     /locus_tag="ACG9BB_001951"
     CDS             10318..11847
                     /locus_tag="ACG9BB_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFN2837883.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            11765..12817
                     /locus_tag="ACG9BB_001952"
     CDS             11765..12817
                     /locus_tag="ACG9BB_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFN2837884.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            12819..13136
                     /locus_tag="ACG9BB_001953"
     CDS             12819..13136
                     /locus_tag="ACG9BB_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837885.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            13263..13886
                     /locus_tag="ACG9BB_001954"
     CDS             13263..13886
                     /locus_tag="ACG9BB_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFN2837886.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            13901..14791
                     /locus_tag="ACG9BB_001955"
     CDS             13901..14791
                     /locus_tag="ACG9BB_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFN2837887.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            14814..15029
                     /locus_tag="ACG9BB_001956"
     CDS             14814..15029
                     /locus_tag="ACG9BB_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837888.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            15041..15382
                     /locus_tag="ACG9BB_001957"
     CDS             15041..15382
                     /locus_tag="ACG9BB_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837889.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            15382..15753
                     /locus_tag="ACG9BB_001958"
     CDS             15382..15753
                     /locus_tag="ACG9BB_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837890.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            15746..16141
                     /locus_tag="ACG9BB_001959"
     CDS             15746..16141
                     /locus_tag="ACG9BB_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFN2837891.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
BASE COUNT         6009 a         2393 c         3445 g         4458 t
ORIGIN      
        1 aagaagggga acttaaaagt ctcgtttatg gtgagacaac agaaatttta ctttgtcttg
       61 ctatgcaaga agaacattta gatgaaaatc tcccattgtc agcacatatt atgcgtagaa
      121 ttgctgtgga tgcttacgaa caagcgaaaa atgaagaaga aaatcaacct tcaaatcata
      181 cttttgttat taataacaag gaagatttag ctgatgtgat gactcgtatt ctcaaggggg
      241 aatttcaatg atgaatgaaa aaacaattga tctactcatt attttatttt tcacgttagt
      301 gattgtagcg aaaagttatg taatctttgg cttgttattc gcaattacca tgcttgtgct
      361 ttgcgtgtta gcaagcagaa aagaggttaa acatgaacga gttagcaaat cttgacaact
      421 atttaactga tcctgattat gctaaaccac catatgaagc accaattgat gaggaggatg
      481 aagatgagta aatctacctt agaaatgagc catcaagaat ggcttgaaga ccgtaagaaa
      541 ggcatcggag gttcggatgt tggaacaatt ttaggattga ataaatggaa atctccttat
      601 caactatggc tagaaaaaac aggacaagta gtacttgaag aatcaggaag tgagcccgct
      661 tattggggta atgttttaga agaagtggtt gctaaagagt ttcaggaacg tacaggcaaa
      721 aaagtacgca gaagaaacca agtatttgaa catccattac atccattttt aagagcaaat
      781 attgatcggg aagtagtggg agaaaatgcc attctggaat gcaaaacagc taatcaattt
      841 ctcggtaaag aatgggaagg cgaagaggta ccgcttagct atctctgtca agttcaacat
      901 tacatgaacg ttctaaacaa agactattgt tacatcgctg tcttaatcgg tggtcaaaaa
      961 tttatctgga agcggattga acgagatcaa gagctgatcg atacaatcac tgaacaatta
     1021 gtagagtttt gggaaacaaa cgttcttgga ggtatcgagc caattattga tggtagtcaa
     1081 gcgacagctg atttcttgaa agagaagtat gcagatgttg aagacgttca aacgacgtta
     1141 ccaattcatt tcgatgaact agttgaacag aaaaacgaac tcaaacggac taagaaagaa
     1201 attgaatcag ctatccgtca agtagacaac gagattatca gcgagctagg taaacgtgaa
     1261 gccagtatcg gtatcactca aaaaaacatc atcagttgga aatttgtccg tacgagacgt
     1321 atgaactcga agaaactagc agagaaatat ccagatgtcg caaatgatga agagatttat
     1381 aacgttactg aatcaagaag gctaaccgaa agggagatca aataatatgg caacaaatga
     1441 atcgttaaaa aatcaattgg cagcaaagcc acagaaacaa gttgcaccag gtcaattagg
     1501 acttaaagct ttgatgaata caccaacaat gagaaagaag ttcgaagagg tgcttcatga
     1561 caatgctaat gcttttatgt cgaatgttat gactcttgta tctaatgaca gttatcttgc
     1621 agatagtgaa ccgatgtcta tcatgagtgg tgcgttaact gctgcaacat taaatcttgg
     1681 gctagataag aatttaggtt atgcatattt agttccattc aatagtaaaa acaagcaaac
     1741 aggaaaatgg gaaaagaaag ctcaattcat gcttggctat aaaggatata tccaattagc
     1801 ccaacgatca ggtaaataca aagcattaaa tgtgatcgaa gtttacgaag gagaactaaa
     1861 aagctggaac cgactgacag aagagtttga gtttgatcca aatggtagaa cgtctgatga
     1921 agtcattgga tatgttggct atttcgagtt actgaatgga ttcaaaaaaa ctgtctattg
     1981 gaccaaacaa gaaatcgaag ctcatcggat tgctaacaat aaagatcgcg ataagacaaa
     2041 gttaagtggt gtgtgggcat ctgattacaa tgcaatggca cgaaaaactg ttttgagaaa
     2101 tcttctttct aaatggggaa tcttgtccat cgaaatgcaa gaagccacca catcggatga
     2161 gagagtccaa agggttcaag aagacggcag cattattgct gaaacagaag ttgaagaaga
     2221 tattcctgaa agaaaagaag cagaggttat atctgaagaa aatgaagatg tacaaactgg
     2281 attatttgat gcatctaatc cgccgttaaa caaataatga gggagctttc tcccttgctt
     2341 ttctagagga gaaatacgaa tgaacacagg atatgtcaaa ttacatcgaa aagtgatgaa
     2401 ttcattcgtt tggaccaatc cttatatgta taaattatgg aacttgtgtt tgatgaaagc
     2461 gagtcatgag aatcgcaaga tactttttaa tggaaaagag atacaagtga acagcggaga
     2521 attcgtcaca gggcgcgatg ctatcacatc tgagatgaac gaaggcgtga aacgtgaaca
     2581 tcaagtgaac agcggttctg tatggagatg gctaaaacaa tttgaaaaaa acggaatgtt
     2641 gaacatcaaa tcaactacga aatacagcgt tatatcaata aaaaactggt ctttgtacca
     2701 aagcagtgaa caacaaatga acatcaaacg cacaacaagt gaacaacaag tgcacacaaa
     2761 caagaatgaa aagaattata agaatgaaaa gaataataaa acatcatcgt cgcaaccacg
     2821 caaaaagcgt gtttacgaca ccgactcagt ttactacatt ctcgcggagg agttattcaa
     2881 acagatttgt cagaatcagg aaatcaaaaa gccgaatctg caaagttggg cagataacat
     2941 tcggaaaatg atcgagatcg acaagcggac cgaaaaccag gtacggggaa tgattgaatg
     3001 gagtcagcac aatgtgtttt gggcatcgaa tattttatct gctaaaaaat tgcgagaaaa
     3061 atacgacaca atggcagcgc aagccaatcg tgattataaa acaaaacaaa ctaaaacgct
     3121 tgaatacgag aaatttagca cagatgagtt gcctatttga gaggaggcgt aggcatggaa
     3181 actgttgggg aaatcatgga aaagctgata cagaaagtgc ttgtccaacg tggcgaatgt
     3241 cctgaatgcg gacagccttt gtatggatgg cgtacgaaga accctgatgg ctcagaacgt
     3301 tgtaagccaa cctgtatgca atgtgggtat aaagcattgc gagttcagga agacttacaa
     3361 acagaacgaa tttacaacga gagcctgaaa gcaagagcaa tcaatttttt caaaggtggt
     3421 tctgttgtgc ctaatcaagc gttgtttgat tgcacattgc agaattatca aattgtcgat
     3481 caagaaacaa gacaagcggt tgaagtaacc aaacgctttg ttaattcagt cttgttagga
     3541 aatccaagtc accttgtttt aactggaaaa caaggaacag gtaaaagtca cctagcgatg
     3601 gcagcggctt gggaagtctt gaagcgatca aactacgata aaaaaatctt atttatcggg
     3661 ttacaggaaa tgctggatca aatcaaattt tcgtacaaca atcctgaact cagaaaaacg
     3721 attgagggat cgttgattgc agatatcaaa acagcggatt tagtcatcat tgatgatatc
     3781 ggttcagaac taggaaaaga tgcatcagat agtcgagcgt ttggcataaa cacgctaaat
     3841 tcgttcttgg atgcacgaca gaacctagca acgattatca caacgaactt gcttggtgaa
     3901 gaactgaaaa aagcttacgg tacgagaaca atatcaagaa tgtttgtcaa ctctgatgga
     3961 tttacgatgg tattttctca aacagcagac aagcgcataa aaccagtcaa aggagcgatc
     4021 gcatgaattt gactaaacaa gaaaaaagaa tctgtcgtca gataatgttg caaattatag
     4081 gtgatcgtga catggacgaa ttatctcaac aagagagtcg aatggttcgg aagataagta
     4141 agctaattat ttatcgggat acagaaagat ttctcactgt tttaggtaac gtctacgaga
     4201 atccagagtt attggaggta gagtaattgc caaaaatatt agatgcatgt tgcggtagta
     4261 gattgttctg gtttgataag aacaattctg acgtaacatt tatggattgt cgtcagcagt
     4321 atgaagaatt atcaacaggt catgtgatca atgttgatcc agatgtggtc gcagactttc
     4381 gcgatatgcc ttttgaagat gatgtgttcg atatggttgt atttgacccg ccacacttaa
     4441 gacatgtcgg cgaaaactca tggcttgcca aaaaatatgg aaaattagac gaactttggc
     4501 cagaagacat caggcaaggt tttgcagagt gtatgcgagt tttacgacct tcaggaacat
     4561 taatttttaa gtggaacgag gaacagatac cactttctga tgtgttagag gctattggag
     4621 aacagccatt gtttggtaat aaacgaagca aaacacattg gcttgtgttt atgaaaaaat
     4681 tcagctaacg gaggaaacaa aggaggaaaa gtaatgaaga ttgtattaaa taaatgtttt
     4741 ggtggctttg ggttgagcca tgaagcaaaa atggaaattt ttaaaagaaa aaatattgaa
     4801 gtattcccat acatcaataa ctttagctat gactcagatg atgaatacac gagatacaca
     4861 ggccaaaagc taggttcgat ggattttatc tactatttca aaaaagaccc gaaaatcgac
     4921 aaagttacag gaatttatag cgaaattgac cgactgtacg gaattgctga tgattccagt
     4981 ttttcaagtg attcaaacag aggtgataag gacctcgttg ctgttgtcga aaaactagga
     5041 agtgaggcca gtggaccata tgcaagtctt aaagtcgttg atatacccga tggggctgag
     5101 tgggaaataa gtgattatga cggcgttgaa actgcacatt atggttttca aacaggtagt
     5161 atttagtcca ctaatgacaa ctaaagagga ggcagaagga tgagtgaaga tgaatacttt
     5221 gaacagtagt ctattaacga cagagatttt taactggaaa ggggtgtagt catggacgca
     5281 tcggaagtag taaataaaat taatagtctc aaagaggttt tcggagacgt cgaagtagtg
     5341 ataaacgcac aaggctatga caacggattt tttaagcaga ttaccgacat tagcattcag
     5401 caaggaatgg aagacgaaga tggaaacttt atcgatgaag tagccatttt agtgacttgc
     5461 gagtaagtaa ttgacagcta aagaggaggc agaaggatga aaaaattgca ccaactcata
     5521 tctgagaaag aaagcgaact ccaaaattta gaagatagtt tgggattggg gtttccaatt
     5581 gttgagcaag ctaaaatgac tcaaatcagt catctacgac ttgaattgga agatttaaga
     5641 caaatagaaa aatcaattca actcaacgac aaccagaaaa tcgtgttgga gtggttgaaa
     5701 agcgagacaa ttttaactag agaagcacca atattatctg ttaatgcttt ttctgataaa
     5761 aatttactag gaaaattacc tgataaagta cgcaaagctt ataaactatt ggcttgtaaa
     5821 caagaatatg aagtcctatc agcattcgct caatggggat tagaacagga ggaagcggaa
     5881 tgaacaacag acatcgcaga ataacaaaac taagaaaaca ggaactgaat gtactaaaga
     5941 caaagtttga aaaagaatat ggaatttcag cagaagaaac atataaagtg gcaagtcagt
     6001 ttgttgctga tgcgagtaat gctattcgta agtttgggat ttcgatatta aatgatgatc
     6061 gtaaatggga ggaaatgaga tgaaaataaa agacggattt tacgctagta gtcatggtat
     6121 cggcggttta atgctagaca tgccgataaa gaaccctaaa acacgtcaga aatcaaaatt
     6181 caaaatcggt gacatggtcc gatgtaaagc agaagagttc atctatccgt ttcgtggata
     6241 tgtagagcat gtctataatc attcagcaat cattcacatt gaaaacacga tggaatgcga
     6301 taagtggtta gcgaaaagca aagataattt agcagtggct cgtttggtgg atatggaact
     6361 aatcaatgac aaataaaaaa gccggatcgc tccgactgat tcaataaatc caacacattt
     6421 attatatcac ataaaggagc ggtttgactt gatgcaattg ttacgagagg tagatttcaa
     6481 acagacaaga tgtaatgcga gagatgtgct gaagaacttt cggcgtttgg agcggatggc
     6541 aggtcgctct ttgatagata ttaagtcgcc gattattacg gatatgccga aggcaccgaa
     6601 gcacggcaat aaggcagaag acgcgatcat tcagatgatg gatatagaag cggagagaga
     6661 cgcaatacta gcggctttga tggctcttag tctgattagc cgtcagatac tctactacag
     6721 cttctgtgac gtaaacaagc actctaatta tgaaataggg caattgatac gaggatacgg
     6781 agagaagaat gtagagaagt tgaaatccat cgcattaatc gaatttgcag aagcatacaa
     6841 aaaaggcgtg ttagttcagt atcgttgatt ttgtagggtt tttgtaggga tagtgtaggg
     6901 tttttgagcg gtttaacgtg atattatggt agtgtcgaaa gattagtgat aggtctgaga
     6961 caaaataaaa tgtaagggag gaaatctccc tcatcgtttt aacttaagct tcgatagaca
     7021 gcaacggaaa tattaagaat aaggatgtga atttcaactc cttctaaatt gttcttatta
     7081 tctatcatcc gttgttgtct attaatttat gcatcggagg aaaaacgaat ggataaagaa
     7141 atcaaagtaa ctgtcaaatt agatttgact gaactaaaag aactgctcaa caaggctagt
     7201 gaccaagtcg aacagttaca agaaacttta gatgaaattg ctaattttaa aatccaagtt
     7261 tcttagcagt atatttttta gcagctgcac tcataaaatc ataccaagta tcagaaaaat
     7321 ataacttaac taaataatta aaaaaggtgg gtaaatagaa atgacggcgt taataatttc
     7381 aatttttgcg ctctgtctta atgtctatat gattggattt aaaaatggac aaaataaaaa
     7441 atagtagcag ccgagaataa ttttatagtg tcactgtggc ggaaatagaa gacgcaaagg
     7501 tggaggtagg aagcgtatgg cgcactatcg ggaccttgta caaaactaga aaggactagt
     7561 acgtgtgtgg tgcgagccct agggaggaac aggataaccg cctgtgtgta ggttgctatt
     7621 acatattaga tcactctttg agtggtcttt ttatttacat aaaggaggct acataatgag
     7681 aaactactgg tatatatcgc taactaatga atatcctcga accattgatg attgttcagt
     7741 gcgtgttgtg cgttctgtcc aaatcaaaaa gaagtactcc attgttgaaa tgaccagaga
     7801 agctacgcca aaagagattg ataaatgcaa acttctttat tgcggtcatg gtttctatga
     7861 tgaaccaaac attcaaaata atattaacaa gaatttgagg gattagaatg caagaaatgg
     7921 cttaaaaatc tccaatcagg acaaatcaag ttagacaaag tttccgattt aaagatattg
     7981 attgaagctg atttaatgct aaaagatatt gaaaattaga aaacaaaact caacctaaga
     8041 agattgcgag gtggtgtgta ttgaatggca agacaacgtg atccaagacg tgatgaagcc
     8101 aaaagaattt ggttagaatc caacggagaa aagcagttaa aggaaattgc atctgaatta
     8161 aatgtttcag attctcaggt tagaaaatgg aaatcgcaag acaaatggag cgctgaattg
     8221 aaaagtaacg ttaccaatgg caaaagtaac gttactaatc aaggtggcgc tcctattggt
     8281 aatcaaaatg ctaaaggtaa caaaggaaac agcagagcat caccgccaaa gagaaataag
     8341 aatgctctta aaactggtga gtatgaaaca atattttttg acacgttaac cgataatgag
     8401 aaggacatct attctagttt gaatgatgat ccttcttttg ctttgtccga agaaatacga
     8461 cttcttaaaa taagacaact tcgaatgatg aaaagaatcc aacaagctga agctggacta
     8521 aatgatgaag aagtcgaacg attgcagcag ctaagaaaga ttaaaactcc gattgaaaaa
     8581 aatggtaaaa agctagaaat caagcgtgag gttatgcaag atgtgcagat tagcagaaaa
     8641 aaacatcgca aaattgatga tattctttca attgaagatt cattgactcg gattagcaac
     8701 cagttagcta aagccatcaa gcaaatgaat gaactttata tgaatgaata cagaactgat
     8761 ttaattaaag ctcagactga taagatccaa gctgagacaa atgaaattgg cggaaataat
     8821 tcaggtgaag aaatagaaga atggaaacag gcagttttaa atgccgcaaa caaacgggcg
     8881 gtgaaagaaa atgaataatg aatttattcc ttttgctgat attggttctg ccattgatta
     8941 ttactatgat aaaccagtag ctttttgcca agatattttg catttgaatc ctgatgaatg
     9001 gcaagaaaat gttttaaatg atttagctga attttcaaag gtttctgttc gttctggtca
     9061 aggagttgga aaaacagcat tagaagcagg agcaatactt tggttcttaa cgtgtcgacc
     9121 ctacgctaaa gtaatagcaa cagctccgac aatgaagcaa ctttacgatg tactttgggc
     9181 agaggtagct aaatggttaa atgatagctt gatcaaaaac ttactgaagt ggacaaagac
     9241 caaaatttat atggttggtg attcagagcg ttggtttgct acggctagaa cagcgactaa
     9301 accagaaaat atgcaaggtt ttcacgagga ccatatgttg attgtggtag atgaagcttc
     9361 tggtgtgtct gatccaatta tggaagctat tcttggtacg ctatcaggtt ttgataataa
     9421 gctgttgatg tgtggaaacc ctaataatat tgaaggtgtc ttttacgatt cccacaattc
     9481 agaccgtgat aaatacagag ttcataaagt atcaagctat gatagtaaac gtacaaacaa
     9541 agacaatata gaaatgattc ttaaaaaata tggaaaagaa agtgatgttg ctcgtgtccg
     9601 tatttttgga gaatttccca aaggtgcgtt ggattcattt atcagtcttg aaacggttga
     9661 attggctaca gaaaaacaaa ttagtgattc tttagtcaat aaaacaacgg ttgctcatat
     9721 tggtgttgac gtagctcgat atggtgatga ttctacgatt ctctttccta gaattgctac
     9781 cagggcattg gagtatgaga agtattcaaa acgtagcact atggaaacaa caggatatgt
     9841 catcaacatg gccaagaatc taatgagtca atatccgagt attgataaag tgatgattaa
     9901 agtcgatgac actggtgtcg gaggtggtgt aaccgaccgc ctagaagaac ttatagaaga
     9961 caagcattat ccttttgagg tgtttggagt gaataacggt tcaacatcag aagacgattt
    10021 ttacgataat ttaggtactc aactatgggg aaacatcaag gaaatgttag aagaaaatat
    10081 gacagcaaat cttaacggag aacagcctgt tattgaattg ccttctgata gttcgttaat
    10141 caaagaatta agtactcgca aattcaaaat gacaagtaga agtcgtatac gtttagaaag
    10201 taaagatgat atgaaaaagc gaaatattgg tagtcccgat attgctgacg cactggcttt
    10261 agcgttttat gagccaccaa gtcactatca atttattcaa ttttaggagg tgagtttttg
    10321 attagtacag tattatcttt agagagatat aagaagctac gtgttaaata tgcaacacaa
    10381 attgaagatg gaatgtttga tccgaatggt tttatagaat atatgaaacc attttttgct
    10441 gatcgcgaaa gaaaatatct agcttatact agcgaaaaga atgaaataga caatagacca
    10501 aagcctaaca ctgatattgt aaaagttaat aataaacttc atgctggtat gtattcaatt
    10561 gtcgtagacc aagcagtcaa tcattttact ggtataccta tcaaatggga ttacgatgta
    10621 tcggaacaaa aaagaacact cattcaaaga ttaaaagata aattcttaaa aaatgatata
    10681 gaacttccaa cagttccaga agcttttaac aaattaacaa gcaatcttga ttccatgaga
    10741 ttcgcaatgc ttgattctga aactgcaact tttcaaggag cttgtggagt agctttccga
    10801 ttgttagagc ctgtggaaga agatgatggt tggaagttaa gagcaagtaa tattgaacct
    10861 tggagagcag aaagatatgg aaatgccgga atctatatca aagaaaagta tgactcttac
    10921 cagaaaaaat tttttcaaga aatgaaagtt atgacaagaa ataaaattct gacatatgct
    10981 tgctatggtg atttgaattt tgttacaagc ggaacgttca aaaaaattga tgagaccgat
    11041 aaccctttgg ggacgattac cttggcagaa tttaaaaata atacgaatcg ttattgtgat
    11101 tttgaagtag ctgaggaaat tggagatgca attgatcgtg ctttgtctga tcaacaaaac
    11161 gaaatcgaac aatttaagct tgcgtacatg cttgttactg gcacaacaat gagcaaaggc
    11221 actgctaaag aaatgatgaa tcagctaggg attatcaact taaaagaccc tactgcaaaa
    11281 gctgaatatg tcacaaaaaa attagcaaaa gatttcaatg aatatcatat ggatctattg
    11341 aaaaaacagt tttacaccat ttgtaaagca atcgatttta acgatgaggt gtttaaatca
    11401 aacagttctg gagaagctcg caagtggcaa atcattagcc tagaagcgaa aacaaatact
    11461 aaagagcaat attttagaga aggattgaaa gaatgcgcag aaacaattgc tgcttttctt
    11521 aagtttcatg acaaagtaga aattgagcct gaaaaaatta tttttacttt ctctagatct
    11581 ttgccaactg atttaagtta tttagctgaa gctttaccta aacttgcacc atacgtatcc
    11641 aaacgaacta ttcagagcca aattccattt gtaacagatg ttgattatga aaatgaaatg
    11701 atggaattgg aaagtggaag tgcttatcca gatagtgaat acaattttgg cggaggtggc
    11761 aatagtgacg atagaaacga agtattggac caaacgtcga gaactggagg atcaagcaag
    11821 gctcaaacaa gaaaatcaga cacttaaaaa attaactagt gtatttcctg aagcactgaa
    11881 agagatacaa gcaaaactat tatcacaagc tgacttacac aatatcactt atccagaaat
    11941 gatggagttt tacagtacaa gtaatcagaa aaaatatcgt gaatatgtgg aaaaaaatta
    12001 taagtcatta aaaatgtatg atgcaaaata caaagagttt atcgatgaat tttttccacc
    12061 atttgactat gcaaaagtca atcgcttatt acaaatacga tcagatgtat ttaaaattct
    12121 tgcagaatat gcgatggatg cagatgtgaa tcaatatttt tctgatcgct tagaggaaat
    12181 tcttcaaaga acatattctt ctaatgctaa tgtttttgtt caacttttaa acgttgatat
    12241 accaaattat ttaccagaaa atgaaattaa acattatttg aactatccat ggtcaggaaa
    12301 gacattttca agaagacttt ggggaaatat ttcatcgctt gaacagaaac tctctaatgc
    12361 tattgtaaaa agtgttgcta gtggagaagg cgttatacac gcattaaaca ccatgagatt
    12421 agactcagaa atttgtgaca tgtttaagtt agaagaatca aagtataaca aagcgataga
    12481 aaatctcgtt cgaacggagt atgcaaaatt tgcacaagat ggtattgaaa aatcatattt
    12541 agaaacaggt attgaggaat acaacgtatt gactgcaaaa gatgagagag tttgccggat
    12601 ttgtggagga aaggcaagta agaatcccta taaactgaaa gatgctgtca taggtgaaaa
    12661 tcgagcacct ttccatagtc gttgtagatg tacggatgtt cctaatttac caaaattagg
    12721 aaaggatatt gatgaagaat atgaccgttt atttggcgat ttattagatg agtttgcaca
    12781 tgattctttt ggaattaatt tgaaacggag gaagtagaat gaaagatttt tttgaagcag
    12841 tactaacaat taatgtaaat gctgatattg cagaagccta caaaacagct attgagtctg
    12901 agaaccatcc taatggcttg agagaccatt ggaatggcaa ttatgcctac gtggttattg
    12961 gcgatcaaac tgttaattat caagataata ctccagttga taagaatacc gttaatttaa
    13021 cgattcaatt attatctcat tcattaccaa atttaaaaga aacagttgat tggtatgaaa
    13081 agatgggatg tattgttgtt agaactgact acaaagaagg aaagtctagt aattaggctt
    13141 ttttattttg tccgaaatga cgttaaacta gcgcaatact gggcttggtt gaatggtggg
    13201 gcgcaactat taaaactcaa agcaatgcgg ggcgtgaaaa cgaatcgtgg ggcgaaagga
    13261 gaatgagcat gaaacacaaa tcattaatgc caatgaattt gcaatacttt gccgaaggtg
    13321 acgatcaaaa gttttctttt gatgacttca aatcttttgt ggaatccaat gaagaagctc
    13381 aaaaatttat tcagtcacaa tctcaaagtg ttgccgacaa acaattggaa gcttggaaac
    13441 aaaataactt agataaaatt aaacaggata ccatcaagga atatgaggaa tctaagaaaa
    13501 acaagtcacc tgagcaaatt caactggaaa aactacaagc tgaatttgaa gcagaaaaag
    13561 cgttgcgtgt gacaagtgat aataaagcat ttgttgcaga acaaattgct ggattagaac
    13621 tagatggaga gttaaaagag tctatttctc aatttatgct aaataatctt gttagttcgg
    13681 atacagattt cactaagaat gctgttgaag gttttacaag tgttttgaat gcaattaatg
    13741 agaaacatgc agatgcatta aaagaactac aaatgaagtc tgcatttggt ggaactcaac
    13801 aatcgaataa ccaggttcag cagaacaatg aaacattaac aaatccagaa gaacaattag
    13861 gacaaattct tcaacaattt aactaggaga gtgaaaaatt atgaaaaaaa catctttaaa
    13921 taatttagag tatttggata tttcaccagc gattaatgct atgcaagtac caaatacacc
    13981 ttttttaagc tatttacttg gtgctggaaa aacagagcca gcaaactcga cagaaattaa
    14041 atggcgtgaa tatgatatca acaacgatga ttcttctgaa aaacttgagg gcggagaata
    14101 tccagatgct gaatcaggtc gaacttggtt taacaactat actgaaattt ttagaaaatc
    14161 aacctctgta tctggtacat tagatgctat taatgtgaat ggagtcggaa atgaattaac
    14221 taatcaagta gcactacgtg gtatggaaat gaaaattgat ttgaaccgaa aattgattac
    14281 tggtgtaaaa gctgatgaaa atggttctaa aggtcgtcga atgaatggga ttttgaactt
    14341 gatcaattca gcaaataagg cagaaacagc cactgcgggt gcagtaacaa gaaaagatat
    14401 cgatacttta tttaaattga tgtatgaaaa aggttatatg ggagaaaaac tatgcttgat
    14461 ttctccagat atgcaggagt taatgactga tgagttagat ggaaaatcaa caaaaattgt
    14521 tcagttcggg gaaagagtaa cttttggatt gcaaattgga aatatcgtgt ctaattacgg
    14581 tacaggtatt gctctactag aaccatcatt gccaaaagga acaattgccg caatcgatac
    14641 taattatgtg aaactacgtc cattacgtga atggagagca gaagaacttg caaaaacaac
    14701 ggattctaga cgtatcggtc ttgtaggtga atactctctt gaatacaatg cttcaaactc
    14761 tggggcaatt ttaaatttaa agtctgaata aaagggagtt agtactccct tttttgatag
    14821 gaggaattac agtggtaaaa aaagatgaaa ctaaaaaaga tgaagtcgtg aaatatagag
    14881 taggtaaaac taaaaatttt gttgggtttg ttcatcctaa aactcgtaga tttatcacag
    14941 cagattcaaa taacgaattt atcatttcta tagatgataa agaagcaatt gcaattttgg
    15001 aagatgcaat tgatgttaat gaaatttagg aagtgatctg atggatgaat cgctaaaaac
    15061 ggaaatcatt gagtctacaa aagaagattt tccagatttg agtgaagaac gcataactaa
    15121 tttattagaa ataattttgc tagaaattga atcatacaac acttgtaaaa atgatatttc
    15181 atgggaaaag ttaaaaagcg tgattagtga agtgttgtat aaaataataa aaaatgaatc
    15241 agaaaaaaca gtatcttcag ttagacgtgg cgatacaacg attagttatg cttcaacaac
    15301 aaatgatgtt agtgagttac ttctaggcta tggtgatttg atacgaagag ttattggttg
    15361 tggaggattg gagttttttt aatgaatgaa gcagatattt tagaaactac ttatgaagat
    15421 agttgtatta ttgaaagact gacggacatt gaagattcta atacaaatat tactattcaa
    15481 gattacaaaa aagtatatga taatcctatt tcttgcgctc tttcacaagg tcaaatcgat
    15541 ggactagcag tcatagaaga tggagagatg gtaaatgttt caactgacac atataaatta
    15601 tttgttcatc ctaagattaa actaaaaaaa ggagatcgaa taacaataac tcaaaaggcc
    15661 agtggcttaa ttttttctct atttgctact aagccttttt actatcctag tcattgtgaa
    15721 gtgaatttga taggaagtga gaaaaatggg taatctcaaa tatgaatcaa atgcagaaaa
    15781 gatcattgag aattttaaaa atatgactgt aattgctcaa aaagaaggaa tatccttcgt
    15841 taacgattca atgaataaag tcgttagttt agctaaacca ttaactcctg taaaatcagg
    15901 taatttaagg cgaggatatc gtgtagtaaa agctagaaaa ctatcaagcg gtcgaattgt
    15961 tggagcagtt ataaacaatg aacattattt taaatatgta gaagaaggtc ataggactaa
    16021 aaatggtggc tttgtcaaag ggaaattcat gttaactcgt gcaacgaatc ttgcaaatat
    16081 gtcttatatt cctcgaagat ttaaacaaat ggcaataaaa atcgttaaga aaggaaagta
    16141 acatgaaaga tgaaatcatt gctgcaatca gtagcaaatt aaaagaaatc tatcctgatg
    16201 ggacaatata tcttgattca gttatgcagt caactaaaga tttttatttc gttttatcag
    16261 taatggaatc tggaactgaa aatgtaggaa ttgatattca aaatg
//
LOCUS       DAZAFF010000064        16163 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000064 DAZAFF010000000
VERSION     DAZAFF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.064"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..197)
                     /locus_tag="ACG9BB_001960"
     CDS             complement(<1..197)
                     /locus_tag="ACG9BB_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837892.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKK"
     gene            complement(185..814)
                     /gene="pyrE"
                     /locus_tag="ACG9BB_001961"
     CDS             complement(185..814)
                     /gene="pyrE"
                     /locus_tag="ACG9BB_001961"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFN2837893.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(821..1537)
                     /gene="pyrF"
                     /locus_tag="ACG9BB_001962"
     CDS             complement(821..1537)
                     /gene="pyrF"
                     /locus_tag="ACG9BB_001962"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFN2837894.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1534..2466)
                     /locus_tag="ACG9BB_001963"
     CDS             complement(1534..2466)
                     /locus_tag="ACG9BB_001963"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFN2837895.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2463..3239)
                     /locus_tag="ACG9BB_001964"
     CDS             complement(2463..3239)
                     /locus_tag="ACG9BB_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFN2837896.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3293..6478)
                     /gene="carB"
                     /locus_tag="ACG9BB_001965"
     CDS             complement(3293..6478)
                     /gene="carB"
                     /locus_tag="ACG9BB_001965"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFN2837897.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPPTQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6483..7559)
                     /locus_tag="ACG9BB_001966"
     CDS             complement(6483..7559)
                     /locus_tag="ACG9BB_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFN2837898.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7564..8844)
                     /locus_tag="ACG9BB_001967"
     CDS             complement(7564..8844)
                     /locus_tag="ACG9BB_001967"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFN2837899.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8863..9789)
                     /locus_tag="ACG9BB_001968"
     CDS             complement(8863..9789)
                     /locus_tag="ACG9BB_001968"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFN2837900.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9802..11085)
                     /locus_tag="ACG9BB_001969"
     CDS             complement(9802..11085)
                     /locus_tag="ACG9BB_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFN2837901.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11099..11638)
                     /gene="pyrR"
                     /locus_tag="ACG9BB_001970"
     CDS             complement(11099..11638)
                     /gene="pyrR"
                     /locus_tag="ACG9BB_001970"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFN2837902.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11873..12784)
                     /locus_tag="ACG9BB_001971"
     CDS             complement(11873..12784)
                     /locus_tag="ACG9BB_001971"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFN2837903.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12781..13245)
                     /gene="lspA"
                     /locus_tag="ACG9BB_001972"
     CDS             complement(12781..13245)
                     /gene="lspA"
                     /locus_tag="ACG9BB_001972"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFN2837904.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13250..13948)
                     /locus_tag="ACG9BB_001973"
     CDS             complement(13250..13948)
                     /locus_tag="ACG9BB_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFN2837905.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13935..14321)
                     /locus_tag="ACG9BB_001974"
     CDS             complement(13935..14321)
                     /locus_tag="ACG9BB_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837906.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14468..16138)
                     /locus_tag="ACG9BB_001975"
     CDS             complement(14468..16138)
                     /locus_tag="ACG9BB_001975"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFN2837907.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
BASE COUNT         4350 a         3726 c         2885 g         5202 t
ORIGIN      
        1 ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa aaactgatca
       61 cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag tttttggatt
      121 tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc ttcatcaagc
      181 gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga gttaattctt
      241 gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa ttggtcaacg
      301 tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac tcatacgtaa
      361 agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca gctgcttcca
      421 agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga ccttcaaata
      481 tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga atatagacca
      541 ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct gttgcagttc
      601 cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat ccttttgcga
      661 tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa attgggcttt
      721 tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct tcaatttcta
      781 ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat tctgtttgaa
      841 tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct ataacgataa
      901 aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga tctcctgcga
      961 ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct gccatttctt
     1021 tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct tttttggcac
     1081 agctagcata atggatcaca cttttttcta aagaaacttc gattagttga tcggcatgca
     1141 tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga cgttttccac
     1201 cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga ataccgccag
     1261 cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt gctttttcga
     1321 ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca gcctctttta
     1381 gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt ttcacaaaca
     1441 atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta gttgaaaaat
     1501 ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct ctctcacttc
     1561 tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag ggagctcttt
     1621 aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag ctacagcact
     1681 tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc ccatcccaat
     1741 gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga caggcttgat
     1801 tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg tcttaaggtc
     1861 aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc cagcttcgat
     1921 tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat agacaggaac
     1981 agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat ctgttccaaa
     2041 agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg ctttcacatt
     2101 tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag caccagcaac
     2161 atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt tatgcataac
     2221 aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg gtgtttccgc
     2281 cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga ccatgatcga
     2341 tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga aacagccgct
     2401 tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta aaggattctc
     2461 attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac tttcacactt
     2521 tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc ccctatccca
     2581 catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa cagctgttcc
     2641 actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc cgtcttcgtt
     2701 cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc agtagcgatt
     2761 cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg tgtggcaaaa
     2821 cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa ctcatataga
     2881 ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg aagattggac
     2941 aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc agaaagagta
     3001 gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt atttgtttcg
     3061 tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg cggcacgaga
     3121 atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt caatgtcatt
     3181 tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc ttgtttcatc
     3241 tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc cgttaaatag
     3301 ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct gtgtctaaag
     3361 aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa ccgtctttag
     3421 aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta ccatctctta
     3481 ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact gtttcaacga
     3541 ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga ctatagccaa
     3601 tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc gcaatcgtaa
     3661 ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc ttgtacaaag
     3721 ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt tcaggcccta
     3781 agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta acatgaactt
     3841 cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc tctcccaaaa
     3901 tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag aatggaacag
     3961 tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg atgacaaact
     4021 ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg taatcttcga
     4081 tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt gaatcccctg
     4141 aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact gtttcaccgt
     4201 cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact agtactggat
     4261 gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc tggttttcaa
     4321 cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact ggataaccaa
     4381 ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct aaaggttgag
     4441 gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca cgatccaagt
     4501 cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg gctaagttga
     4561 ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc tcgatcacat
     4621 tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata gaaaagtcag
     4681 tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc tgctggattg
     4741 ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata cggataggac
     4801 cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt tcatattcat
     4861 aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg tcaaccatct
     4921 tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct tcgtttattt
     4981 cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt ttcaatatct
     5041 tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc aacagtttat
     5101 ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg taccctcttc
     5161 taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact ttttggatta
     5221 ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta ccgatttcta
     5281 aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc atcacttcac
     5341 cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta tcaaacggcc
     5401 agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca aaagtcgttc
     5461 ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta gcagctagct
     5521 tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa acacgcggat
     5581 tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg acattacagc
     5641 ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc atttgatatt
     5701 catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta tggataccca
     5761 ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa tctctcatca
     5821 cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat tgggtagggg
     5881 gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt tcacacatac
     5941 ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag ccgattttat
     6001 ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg attggctgat
     6061 tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg atcgcagaaa
     6121 gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct gccagctcca
     6181 ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct ggacgctctt
     6241 ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact tgatctgcca
     6301 cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact tcatatcctt
     6361 cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg gcttgaccaa
     6421 tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt ttcggcatct
     6481 gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa atgtagtgca
     6541 tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg ataatctctg
     6601 tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat gagtttttct
     6661 ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa atcaattctt
     6721 ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt cattttatag
     6781 gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc aaaaatcggt
     6841 acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt tggatcacca
     6901 ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc ggcatctgtg
     6961 ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa aatactatgt
     7021 ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg ataaggtttg
     7081 ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag ctgatcaaat
     7141 gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc gacttcacgg
     7201 attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc tttttgtttc
     7261 aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc atgttctttt
     7321 actactactc ctttgcatgt tggagaaatc gattcataat catctctatt taccccataa
     7381 ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg atcagtgata
     7441 gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac actagcatct
     7501 gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa acgttccata
     7561 cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt gtgtttcgcc
     7621 ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt cttctttatc
     7681 gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac caaccgttag
     7741 cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat tgatcacttg
     7801 ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa acgctgtttc
     7861 gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt ctaatccatg
     7921 gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt cgatcaaagc
     7981 ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga aatcttcggg
     8041 aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta cgtggattcc
     8101 tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat ggcaaacatg
     8161 gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag aagactcggt
     8221 tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt gcataacgcc
     8281 gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt tttttgcagc
     8341 ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt catttgtgaa
     8401 agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac ttcgcagatt
     8461 ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa tgatgtcata
     8521 tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca tcgcgcatac
     8581 agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct ccttgtatgt
     8641 aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga tcaattgtcc
     8701 ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga ttgctttgat
     8761 tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg tattgatctg
     8821 accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt ttccatttag
     8881 cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct gaacgatccg
     8941 cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa ctggtgctgg
     9001 gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta acccatgttc
     9061 ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt gttgcacccg
     9121 aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat aatgtccgta
     9181 cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt gtgcgcctag
     9241 acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat cacctacgat
     9301 ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca aatcaagcaa
     9361 gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac actggattgt
     9421 tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa ccgctacgtc
     9481 tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt tttggacaga
     9541 gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg ctacatcgaa
     9601 gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt actggcgctt
     9661 ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca agcccataac
     9721 ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac gttctgattt
     9781 gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg gtaagataag
     9841 atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa atgtgcctac
     9901 ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca acagattttt
     9961 ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg cggcaattac
    10021 tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta aaatcaaagc
    10081 gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac cgatcacaaa
    10141 cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac ttgttacagg
    10201 aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca acgtcttatg
    10261 aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca taagatggcc
    10321 gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg ctccaagata
    10381 tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa accaggcagc
    10441 gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt atccgctaat
    10501 aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt tgaagaaaat
    10561 tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg tgccctgatt
    10621 atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca tgatgactgg
    10681 accgacgacg attggaggca agattttatc cagccatgct gaaccgatct ttttgatgat
    10741 cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa tcgctggata
    10801 accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc ttcctaagta
    10861 cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc ctgaactgac
    10921 tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg ctccgaacat
    10981 cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc gatcgtggat
    11041 atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc tctctcctct
    11101 aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc atctctacta
    11161 aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca cggattggca
    11221 attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt ctgccaaaat
    11281 ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca tcatcaatca
    11341 aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt tccggttctt
    11401 ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct acaggaatat
    11461 cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag attcctcgtg
    11521 ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata atctcataag
    11581 taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt gcttgcattt
    11641 tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag gaggaatgtt
    11701 tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca atctacttat
    11761 ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta aacttaaagg
    11821 aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta aattaaaatg
    11881 caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga ggtgcttcaa
    11941 agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca tggagaaact
    12001 gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct aacggataac
    12061 cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt tttaattcaa
    12121 ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc gacttcccat
    12181 cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc gcattgatgg
    12241 ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc aaagaggtct
    12301 tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca accattagca
    12361 gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg ccgttgatcg
    12421 tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc gtatgacctg
    12481 ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct tcatcttcgt
    12541 aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt tcggggatca
    12601 agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc tgtccattga
    12661 cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa tcggtgaata
    12721 aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt ttaagttctg
    12781 tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac aaatcactcc
    12841 acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat ctgtctggaa
    12901 catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg caccagctag
    12961 aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt taatcatcaa
    13021 tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca tcttcccttc
    13081 tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa tacctggtat
    13141 aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga ccaactgatc
    13201 cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat caatcctttt
    13261 ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca tcgggattcc
    13321 catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc gcttcaacct
    13381 cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg ttttggatga
    13441 tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt tcttttgcta
    13501 gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta ttgtataacg
    13561 gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac ggaagatctt
    13621 gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc tgaatcagtt
    13681 cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca atgacaaaat
    13741 cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt aaaagatcat
    13801 cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca agttcggtaa
    13861 atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt cgattgaaac
    13921 tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata tgcccggttt
    13981 tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa aaataaattt
    14041 tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat ttgaagaatc
    14101 aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata aacggggctt
    14161 tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag cgttatcttt
    14221 tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat cgagctaccc
    14281 ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc ctattatgct
    14341 ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac aaaattaatt
    14401 ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg gtagattata
    14461 atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata ttcaatgcag
    14521 caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct actaaaaatc
    14581 ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc ggtgcaccta
    14641 gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc ggcagtttgt
    14701 cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg aaaacaacac
    14761 catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc gtttcttcta
    14821 ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact acagcttttg
    14881 ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg gcaagaatcc
    14941 cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct gttacgaatt
    15001 cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata tggcgcttta
    15061 aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca tcttttttca
    15121 ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca tccggcgctt
    15181 ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct gcaccaaatc
    15241 ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct aaaatactat
    15301 tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt gttccgtaaa
    15361 ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga gcgccttctg
    15421 ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca acgatccttg
    15481 caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc atgatttcgc
    15541 tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc ccacccaagc
    15601 caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc caaatcactc
    15661 ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa agagcagata
    15721 atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag ttgatatctt
    15781 ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt cccatcacag
    15841 gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta ttgagcgcgt
    15901 ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga ttgatggaag
    15961 taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca gaaaaattga
    16021 ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct aatttactag
    16081 ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca ttcttcattt
    16141 gattacccct tttcacgaac ttt
//
LOCUS       DAZAFF010000065        16076 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000065 DAZAFF010000000
VERSION     DAZAFF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.065"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..958
                     /locus_tag="ACG9BB_001976"
     CDS             41..958
                     /locus_tag="ACG9BB_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFN2837908.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            951..2024
                     /locus_tag="ACG9BB_001977"
     CDS             951..2024
                     /locus_tag="ACG9BB_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837909.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            2172..2690
                     /locus_tag="ACG9BB_001978"
     CDS             2172..2690
                     /locus_tag="ACG9BB_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837910.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            2671..3063
                     /locus_tag="ACG9BB_001979"
     CDS             2671..3063
                     /locus_tag="ACG9BB_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFN2837911.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            3139..4074
                     /locus_tag="ACG9BB_001980"
     CDS             3139..4074
                     /locus_tag="ACG9BB_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN2837912.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            4207..4986
                     /locus_tag="ACG9BB_001981"
     CDS             4207..4986
                     /locus_tag="ACG9BB_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFN2837913.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            4979..5296
                     /locus_tag="ACG9BB_001982"
     CDS             4979..5296
                     /locus_tag="ACG9BB_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFN2837914.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            5540..7150
                     /locus_tag="ACG9BB_001983"
     CDS             5540..7150
                     /locus_tag="ACG9BB_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN2837915.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            7307..8155
                     /locus_tag="ACG9BB_001984"
     CDS             7307..8155
                     /locus_tag="ACG9BB_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN2837916.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            8386..8865
                     /locus_tag="ACG9BB_001985"
     CDS             8386..8865
                     /locus_tag="ACG9BB_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFN2837917.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            8898..11126
                     /locus_tag="ACG9BB_001986"
     CDS             8898..11126
                     /locus_tag="ACG9BB_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2837918.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            11255..12898
                     /locus_tag="ACG9BB_001987"
     CDS             11255..12898
                     /locus_tag="ACG9BB_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFN2837919.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(12971..13516)
                     /locus_tag="ACG9BB_001988"
     CDS             complement(12971..13516)
                     /locus_tag="ACG9BB_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFN2837920.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(13539..14024)
                     /locus_tag="ACG9BB_001989"
     CDS             complement(13539..14024)
                     /locus_tag="ACG9BB_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN2837921.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(14014..14826)
                     /locus_tag="ACG9BB_001990"
     CDS             complement(14014..14826)
                     /locus_tag="ACG9BB_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFN2837922.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(14965..>16076)
                     /locus_tag="ACG9BB_001991"
     CDS             complement(14965..>16076)
                     /locus_tag="ACG9BB_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFN2837923.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         5284 a         2910 c         3250 g         4632 t
ORIGIN      
        1 taataagtga aaaacagatg agtaagagga ggcaagtagt atgaaagagc cagatacaaa
       61 tgcaaattat gagccagacg aactagagga gacgaatgcg gatcaggata caggacaaga
      121 atcgtcttct ttttacgata tcaaagaaaa agcaagcatg aatctttccc aattattaga
      181 agacttacag gcttttgaaa aagctgtccg cgaagaagat atccctgaga tctaccgttt
      241 atacaatggc cgattgaatg aagaattaaa aagaagctca aatgaaaatc acgagatcga
      301 tcaattgctg atgcgtaaga tccatgatcg ttttttacag gaatttcctt ttatggaaca
      361 agtagaaaac atctcgccaa cgatgagcta ttacaaaatc ggcacttatt accatgatcg
      421 gcccacggtc ggtattgatg caagtctgcc agaaattttt gttttgccta agatcgatga
      481 agagtgggag aaatattccc aaccggagtc aacggactat gacaaaaaaa tcaatgagct
      541 agacgcaaag gtgatcactg ctcagacaga aattgaacgt ttagatgaac aaataaaaga
      601 aatcaatcga caaatgactg atttgaacga caataaagga ttcttgaatc gtaaaaaaat
      661 cgaggaagaa attcaagaat tagagaagaa gaagcaagta ctatctaatg aaaaattagg
      721 ctggcttcct tatattgaga cgcctgaaac aatccagcag caaaaagaag cattgaagca
      781 agaagcaaga gcagaccagc ttcgtgcagc catcgtagaa aaagaacaaa ggcaaatcag
      841 acgctatttc ggtagcaaag agggattcgg tcaagcaatc catgaattct tgatgaatta
      901 tcttggcaat gagaatccta ataatcaatc taatgaagga ggttccgagt atgaatgaac
      961 acgatgaagc agaatggaca aaaaatgaag aacaaacaga agatcatgaa gaaacgaaca
     1021 cttcattgga agaacaaaca agtaatcctc tggatgacac agaagaaatc cctatgctag
     1081 aggaaacaaa aatacacgaa aaagaaaaag taactccgac gaatacgaat actgcagaaa
     1141 acacccatgc ttacgaaata actgaaaatc ccgggtattt agaaggaaaa gagcaagatc
     1201 atcaagatat cgtcagtttg attgaaacag cggaagcaga attagcggat ttacgtatga
     1261 gaaaagattt gatcgaatca gattttccac aattattaga atcatatgat cgttttatat
     1321 tgaatgaaga accaatctcc acgatcggaa agacaagcct gatcgaatac ttcacgtatg
     1381 aattactgaa accgttgaat gatattggtc tggaacggtc aaattcagct tatgacacat
     1441 ggaaaacaag acatttttcg atcaattata cgttgaatgg acaaaacgaa ttagtctttt
     1501 cctttgtatt accgaccatc gatcaacgat accaagtgga atcgatgagt ctcctaaacg
     1561 tatcaccaga gacaatggaa atcaatgtcc aagatgaccg ggtgctttct ttgatccgac
     1621 actggtcagt ggatcgagta ttctctgcag ggcagatcac gatattcaat cacaaattga
     1681 atcaattatt agcccatgca agaacacttg gttttaccgt taatcagaca ttactagaca
     1741 atacgaagcc acttcgtttg aatcttcaga gcgaatttga attgacgaat gaagtattgg
     1801 atgatatttt tatcgtggca atgaaaaatc catcctacga tatggaaaag atcaacgaaa
     1861 ctacttatca agtacgatta gataaaggac agtctttaac gataacaaca aacgaaaaag
     1921 atcaaacagt cttgtcgatc tcatctggag actacccacg atctgtcatt gatttcttta
     1981 tcaattacga attcttggtc ccattaatgg tgagaaagtc ctgattggat aagttggatt
     2041 accagatgtc attcagataa ttctgttcca aaggacttgc ggcagttatc cagaataatt
     2101 gtcacaagtc ttttctgtat gtactaaatg gacgtgaata acttgctgat gaaggaggga
     2161 tttcattagg aatgtccaca aataaaatag cacagcttag cttattgtct gctgcttgtg
     2221 tagcaggtag gatcgctttt caatttattc cgaatttcca gccaatgact gctatttttt
     2281 tatttatagt actttactta agcttaaaag atgcgttaat tgtcatgagt ctttctattg
     2341 cgatcagcag tttttatttt ggtgttggac catgggtcat cggccaatgg atcagctata
     2401 ctgttgttct gagtttgttt tcaatcgtct gcttgagaaa aacagtgcaa aggaatctat
     2461 ggttaaaggg agtctgtttt tttctggcgg gcatggttta cggaataggt atgaccgttt
     2521 ttgatacgct gttatatcgt ttgcctcaac cttggatcta ttaccttcaa ggggtgtcgt
     2581 ttgatttgat gcacagtata ggcaatgttg tgttctttct tgtttttctg ccagtcgtca
     2641 aacgttttta caatcattca ggaggaaaaa atgaaaaaaa taatttatag tttgcttgca
     2701 atgagtgtca gtatactcat tttgacaagt tgcagtacaa cctatacaca atcggcttcc
     2761 aaccaaaagg aggaagtaaa aacatcgcaa aaagcaacaa tcacgttaca agaaaatggt
     2821 caaaactttt cggaaaaaga agtgttcttc aaaaaaggcg atgatttact gagtattttg
     2881 aaacgaaatt ttgaagtcaa agaagataac ggcttcatca cttcgcttga agggcactca
     2941 caggatgaga aaaatcaact ctattggatg tttactgtgg acgggaaaag tgctactact
     3001 ggtgccaaag agatcaaatt gaatgatgga caatcggtca ttttcaattt gtctaaacta
     3061 taagtttcat aaaaaactgt taaagggcga atattgccat aattcagtga gttcagttga
     3121 tgaaagaagg aaaataacat ggaaaatttt aaaaaaagcg gaatgttttc cgtaattgct
     3181 gctctagggg ctaatattct agttgctatc agcaaatttg tcggctatgc aatcagtggc
     3241 agtgccgcca tgctaaacga aagcatccac agtatcgttg attgcagcaa tcagattttg
     3301 ctgttattcg gtgataaaca agccaataaa ggacagagcg agttgcatca atttggtgaa
     3361 ggacgagcaa aatacttttt cagtacaatc gtcgcaatga tgcttttctt cggcggtggg
     3421 gcacttgggg tgatggaagc ttttgaaaag ttgacgcatc catcccatga agtgggtaat
     3481 ccgatcattg ttatagcaat actgttattt ggtttggtaa tcgaaggaag ttcgttacgc
     3541 gtggcaatga aagaaatcaa agaattgaat actgaaaaac tttccacgat gcgctttttg
     3601 cgcgaaagtc gacacagtga aatactgatt atttttacgg aagacttgtg tgctgtaatc
     3661 ggtttggtct tggctttagc aggaacaata ttgacttatg caaccgggat tgctgcattt
     3721 gatgcgatca gcggattgct gatcggtttc ttgctgatgg gagccgccat tttcctagca
     3781 aaagaatttt atagtctgat catcggtgaa agcgtgacag caagcgattt atcaaaaatc
     3841 aagttagctt ttgaacgtcc tgagatcagt cggctgatcg atataaaaac agttcattta
     3901 agtccaacgg agattttagt tgcagcaaaa gcagatatcg ttggtaacaa agagaatgaa
     3961 gcttactcgg tcatcaatga tattgaaaaa aatatgcgtc agtctcttcc tgacaagaag
     4021 atgtatatat acattgaaac agataaatat gacccaaatt attctcgtaa ataaaacgtt
     4081 tattcatgaa ttgaagcaaa aatgagaaaa atataatctt gtttttaaat aaaacggcta
     4141 caatttagcg aatttatgtt acaatgagtc cgctgtttat ttgttgtaat taaaggagat
     4201 attttaatga acaaaaagaa aagtgcgcca tggcttcaag ctttgcatgc agtgatgcca
     4261 ctttgtataa gctatatacc agtaggatta gcttgcggag tattgttgca aaaagttgga
     4321 tttgatcctc tcttatcagg actgttatcg attctggtct tttccggtgg cgcgcagttt
     4381 ttagttgcat ctatgcttac gacgcaagca tcatttgcaa cgacattatt gatggttttc
     4441 tttttagaat tacgctatac attattagga gcaagtcttg ccggttttat gaaaaaagaa
     4501 aagcggacgt ttttggctgt cttttctcaa tcactgaatg atgaaaacta tgcggtgaac
     4561 tacttaaagt tttcaactga cccttcctgg aataaaagaa aggctttata tgtcaattgg
     4621 ttctcaatga ctgcatgggc cggtagcaat atgatcggta attttttcgg ctcggtgatt
     4681 cgagtagatg ctgaccttgt ccattttgct ttgacggcca tgttcatttt catgtttatc
     4741 atgcagatga aaagcgcact actgatcttt acaggtttat tctcaggcgt attaggggtc
     4801 gtattcatgg tattattcca aaatactttc ggattgattg cagcaacagt cattgcttca
     4861 tttgccggat ttatgctgga gaaggcattc aaaaaagaaa aagctaagaa actaagagac
     4921 cgcggtaaag atgtcaatga gccgctcttc aacttcccgg aacaggaggc caatcaccat
     4981 gactaaacta tatttgctac ttttagttgc ttgtctgttc atcgtggcat atatcccgcg
     5041 cttgttcccg atgctttatt ttactcatcg aaaagtgcca tcctggttca gtgactggat
     5101 gaaatatgta ccggtggcat tatttgcggc gttagctttt aaagatgttt tcattactca
     5161 tgaacatctg gatattgctt ggaatatcaa aatagctgca atgattctag tagcaggcgt
     5221 ggcttacaaa acacgttcga tggcgttgtc agttttgaca ggacttgcgt ctgtcttttt
     5281 gctgtctatg ctttaaactg ttttatttta ttaaaaacag agattgcgac ataagttgtt
     5341 caaactgatc tatccgaact gcatttgcaa cagtcaattc tggatgcaag tttattcgta
     5401 gggataaaaa agattagtgt tggcttatga tgcaagctct tttttatttt aggaattaga
     5461 gagggatttg tccttctatg attttttgct ataataggga agatgtttat tttatgaaag
     5521 aatagttgag ggaatttaaa tgaataaaaa attgatgcaa ggaacatttt ggctaacctt
     5581 tgctaatctt ctatgtaaag ttttaggggt agtctatttg attccctggc taagtatgat
     5641 ggggaacaat caagatggta tgcttgctac gacattgtat aatgttggct atcttcctta
     5701 tggattgttc ctgatgcttg gtacagtcgg atttccaaat gcgattgcaa aaaaagtagc
     5761 tgtagccaca aaagaaggag ataacgctgc ttgtcggctg atttttagaa gtacgatcaa
     5821 tatcatgttt gtcatcggta tcgcctctgc tgcactgatg tatttatttg ctcctttact
     5881 agccggaatc agtccgattg ctaacgttaa taatgggata ctagctatca gaagtttatg
     5941 cccatctttg attgctatcc caattcttag tgcaatgaga ggatatttcc aaggaaaaaa
     6001 tgatctgcgg ccttatggaa cttctttgat tatcgaacag gctgtgcgag tgatcgttat
     6061 cttggctggt acttattact tgcgcgtatt gactgacgga accatattag aagctgtatt
     6121 gatcagtaca gtcgcttctt tcttcggtgg gttagctgcg attatccata tgtatatcgt
     6181 cggacgcaga aaagacttct tcgaacttaa agattttttg atttccagcc gttatttcga
     6241 aagagaaaac cgatctgcct ctgtctctat cattcgggaa acactaccgt ttatttttgt
     6301 cggctcagtc attactatcg ttcaaatgat cgaccaagta acgatgaaac cactgcttca
     6361 ctttttccgt ccagagatcg cagatcagca actggaattc ttattcagcc gtgcttctgt
     6421 caatccaaat aaattgacat tgatcctgat ctccatggta ggaacagtag ctataagcag
     6481 cttgccaata ttaagcacga tgaagaaaaa agatcggata caaatcgaaa aaacagtagg
     6541 agatagtttt tcgattgcgt tattgattct tcttccttca ttgactggaa tgtctttatt
     6601 agctggacca ctatacacat tgttttttgg ctatgatcct gaaagtgtcg gatatttcca
     6661 aatggctttg ctagcttctt tgtttttctc tttattcacg atcctatcta cgatgcttca
     6721 atctttgaat caccatcgtt ttgcgatccg attaactgta gaagcaatta tcctgaaagt
     6781 catcttccaa gtaatcggat taggattagt tggcggatac gggatgagct tatcaaatgg
     6841 cttagcattc ggtattgtct ttgttcgcgg ctatcacttt atgtgcaaag aataccgaat
     6901 agcaccttta gcaaagatca gcaatttttt cttaaaaact ttccgctcca ccctgattat
     6961 gcttgcagta tgttttgccg ttttcttttt gctgaatcag cgattgtcaa tggaatcaaa
     7021 aagctatgca atgatttatt gtgtggctgt aggaagtatt ggcggcttag tatttttatt
     7081 ttctcaattc ggaaaaaatg ggtttcgaat gttgaaaaat gttaaacatc atagcagaag
     7141 caaagaatag gttaactaat cgttttttgc tgtcacttga aaaacggcaa ctcgtatgat
     7201 tacgcttgtc gtttttctat tttccaattt tagtttcata tctaatctta cttatgatat
     7261 agttataagg tctggaaaaa ttctttgaaa cgaggaaatt aaacacatga aacttgcagt
     7321 agttacagat agttcggcat atctgccgga acgtatcaag aaccatcccg acttattcgt
     7381 tattccaatc cctttgatta tggatgggaa aatttataac gaaggaatcg atatcgaagc
     7441 ggatgaatat tatggacttt taaaaaacag taaagatttt cctactactt cacaacctgt
     7501 cgtaggggaa gttttgggtt tatatgagga attgaaagag aaagggtatg acacgatttt
     7561 aagtatccat ctttcttcag ggatttctgg ctttgtcaac acattatttg cgatgaaaga
     7621 tgacatcagc ggagtaacgg tcattcctta tgactctaag atcactagta tgccgatggg
     7681 acacatggta gaagctgcct tagacttaaa tgatgaaggt cagtcccttg atgaaatact
     7741 tgcacatatc gatcgaatcc gagataatac ctacgcttat ctgattgtag atgatttgaa
     7801 caatttagta cgaggaggac gtttgacaaa tggtgcagcg ctgattggca gcttattaaa
     7861 aatcaaaccg atcctgacat tttctgaagg gaaaattgtt ttattcgaaa aaatccgttc
     7921 aagtaaaaaa gcatttgctc gagctgaaga aattattggt agaagaaata acgaaataca
     7981 accgccagtc aaactttacg tgatacatgc aaataattta gaagtagcga ttgcggagaa
     8041 agagaaattg cagaaaaaat atccaaatgc agaaatcgaa attggtcatt tcggaccagt
     8101 cattggtaca catttaggtg aaaaagccat cggcattgcc atttcagcgc aataattcct
     8161 tttattcaaa aaacatacca aaaatattcc aacatcatct tggactttct acatgttact
     8221 aatgagcgtg taaaagaacg agatggtgtt tttatttcta tcaatgaagc tctttttctt
     8281 tgcaatcctc acttcaatcc tctataatgt cgcatatagt ttagtaaatg agtaaactac
     8341 taatttactt atttactaaa aataaaagga tgaggagtta aaataatgga aataccaaca
     8401 aatttaaaac ataaaccagt aatcgttgta gaagactatg ataaagttga tggaagaaat
     8461 gcattaaata cggatgcaaa agggctttca ctgggccttg ctcagtggaa tgatcgtgga
     8521 aaagtcgata tctcaggcaa ggtatggcga catactggag aaaaatggtc acgacagtcg
     8581 gaagaattac caatcacacg tattttagat ttagcgatct tagctgcaca aggaagttta
     8641 tacttccaag atgcttatcg ttatgaaaaa ttttatgatc cagaaaatcc agtcgtagac
     8701 atcattggat tacaagggaa ccgaatgacg gtagaagtag ataccaaaaa tccaaagatc
     8761 gatgaagaca tcattttgta ttacgataca ctacaaaaag acggagaact gattggggaa
     8821 cgattccgta ttttaaaacg tcttttagat gagctaggat attaatagac agagatgatc
     8881 aaaaggggaa gagaagcatg gagaatttct cactaacaga aaaccgaacc ttttcacggc
     8941 tagaaaagca aatgatctgg aagaaaccga aaagccatcg gacaagtgca gaagaatggc
     9001 ggatcgcctc cgaaatcaaa gggaattggc aagatcctga aatgaaaatc agtaacgtac
     9061 ttttggaagg agatgccggg tcggggaaga cacaattagc caaagcacta tctgctgatc
     9121 tgcaacttcc ctatacaaaa gtgacctgtt ttgcagatat ggacaaatca gatgttttcg
     9181 gagccttatt acctgtagta gattctaata aggaacaaga ccaagaatta ctagaagcta
     9241 tttaccaaac agatacactt gaagctgtac tgacactgat ccagaaccat tacggcattg
     9301 atcctttttc tgctaaagaa aaattagcag agctagttca aaggatcgaa gctgattatc
     9361 cagatgcaat ccattacagc tattaccctt ctgaaatcgt tcgagcgatt gaaaaaggat
     9421 atttattaga gattcaagag ccgactgtca tccgagatgc ctctgttctt gtggcactta
     9481 actcagcgct cgaaccaaat gggatgttga atttaccgac aggaatcatt cgtcgccatc
     9541 cagattgcgt cattgtgatc actacaaatc gaaattacca aggaaatcgc ccattaaatg
     9601 aatctttaag agatcggatg caacatgcgg aaaaaatgga tcttcctgcc ttagaagtca
     9661 tggcagaacg tgcgatggcg aaaactatga tccaaaaaca agaacttctc ttaaagatgg
     9721 cagagattat ccgtttgttg gatgaaactg caaaagctaa cgccatcaaa ggtgtagcag
     9781 gaatgcgttc ttatttttat tgggcaaata cctataagca aggacaagat ttactccagt
     9841 cgatttatcc aaaagttttg tataaattat ccactgattc agatgaattg gcaattcttg
     9901 aacaagcttt gtctgacagt ggattgctac tggaacttca ggacatattg agaaaagaaa
     9961 aatggggtca tcctcaatct gagcatacaa aaggaagaac cattagcgcc gaagaagcaa
    10021 atgaacgaaa catcactgaa aatgctgaag aaattctgcg cttagatgaa aagaaatcgt
    10081 tgtctgctga agaaccaaat gagggagaag agaaacaaga agaaacaata gaaaaaaacg
    10141 agattcctcc tcaaatgcaa aaagaacgca gtagcgatat cgaaggaaaa aatcaaaaag
    10201 aacaacaaac aggagatgat tctcaagaaa tgtctgcttc tgacatggaa gcacatgata
    10261 aggaaatcaa gaagcaactg aataaagaag cgcgacaaat catgaaggaa acgattcatg
    10321 aaaaagaagg tctcatcgtt caccgaccag ctttccaagg aaaaacgccg gaagcacgtg
    10381 agctacacat gcaagtaatg ccaatagtag aatcacttag ccggcaaatt ttagagttac
    10441 tggataatga acaaacagag acgtaccaaa aaggaaaata cgaaggtcag cggttcaatg
    10501 caagcaaagt agcatatggt gatttgagaa attttgacaa aaagaacccg ccacatgagc
    10561 aaccttcttt agcggtagct gtacggatcg atgaatctgg gtcaatgata cgggatgatc
    10621 ggatcgaagc ggcaaaaaaa gcagcgattg ccattgccga attttctaag aaagtcgata
    10681 tcccattgtt gatttacgga gatacagcag accgttcgtc aagagaaaag acttctttgt
    10741 tttcttataa agaatttgaa gacggatttc aatggttaaa tgaaaaatta gtcactatga
    10801 aaccaaggca aaacaatcgt gatggtgctg cacttcgttt agcagcagaa aaattaaaca
    10861 gacaatccgc aacaaccaaa ctgttgctta atattagtga cggtcagcca aaagcgttgc
    10921 cggattatac aggcaaaaaa gcaaaagaag atatccagtc tgttttgaaa gaatatgaac
    10981 gtcaaggaat cctatttgtc tccgcagcta ttggccaaga caaagaagaa atcaagtcga
    11041 tctacggtga aagtcgtttt gtagatatta ctgacttgaa tgaatttccc aagcaaatga
    11101 ttcaactgat tgcccgctat ctatgataaa ataaagctca acgaacgcac ttagcagatt
    11161 cgttgagctt ttcgtttcta gttaccggta acaaggatac ttctaaaata gaatcggtta
    11221 taatcaagag ggaccgttaa aggagtgaaa cgatatgata caagaacaaa aatggtggaa
    11281 agaagcagta ggctatcaaa tctatcctgc tagtttcaaa gatagcaatg gcgatggtca
    11341 aggagacatc aatgggatcc gtgaaaaact taattatttg aaagatcttg gtatcggctt
    11401 tatctggatc aatccgatct atcaatctcc atttgtcgat aatggttatg acgtaagtga
    11461 ctatcaagat atagaagaga gattcggaac gatggaagat tttgatttat tgctaaaaga
    11521 agcacatgaa ttagggatca aagtgattat ggatcttgtc atcaaccata gttcggatca
    11581 gcatcaatgg tttgaagaat cgaaaaaatc taaagaaagc ccgtatcgcg attattacat
    11641 ttgggtcgac ggggtagatg gcaaagaacc gaataattgg acatcgattt tcggcggttc
    11701 tgcttgggag tattctcatg aaactggaca gtattatctg catgtttttg caaaagaaca
    11761 accagactta aattgggaaa gcgagaaatt gaaagaagaa ctattcaata tgatccgatg
    11821 gtggttggat aaagggatag atggatttcg tttagatgcg atttctcatg taaaaaaaga
    11881 tgaatattca gtaaaagcca cagagaaccc tttctcaccc tttcaaaatg tctcaggaat
    11941 cgaagaacat ttgaccgatt tgaaacatgt ttttgaagag tacgatatca tgactgtcgg
    12001 agaagcaagt ggtgtcactg cagaagaagg accacaatgg gtagggaaag acggttattt
    12061 cgatatgata ttcgaatttg atcatatcca tatctggcag caagaaaaag aagggcaatt
    12121 agatgtcctt aaattgaaac ttgcattaag cgcttggcaa acttcattgg atggtatcgg
    12181 atggaacgct ttatatatgg aaaaccatga tgttcctcgt gccgtttcag tgtttggaga
    12241 tactcgtccc gatttttggg ctatgtctgc aaaagcaatc gctatgatgt atttcttttt
    12301 gcaaggtacg ccatttattt atcaaggaca ggaaatcgga atgacgaata tgccctttga
    12361 atcgatcgat caagttgatg cagtagattc gaaaagactt tataaaaggc tgcttgcgga
    12421 aggtaagaca agagaagaag cattggatat cattcgtgaa acaacgagag acaacagcag
    12481 gacacccatg caatggactt cggaacaata tgctggtttt tctacacatg aaccttggct
    12541 gatcactaat ccaaatacca aaacgatcaa tgtagaacaa caagaatatg aaccagagtc
    12601 ggtcctgcaa ttctataaga acatgatccg cattagacaa accaataaag gtctgatata
    12661 cggttcttat aaagaatatc ttcatgaaca tccacaactt tatgtctatg aaagatattt
    12721 agaagatgaa caatatctta ttatggtaaa cttaacagaa tcacttgctg actatgaact
    12781 tccaaaagaa gcagatcagt cctggacatt actcttgtca aattcctcta gcggagaatt
    12841 tgaagctaaa ggcatactag caccttatga agctcggtta tataaaacaa acaagtaaaa
    12901 cttcctcgtt aaagttcatt agttggactt tgcgaggaag tttttcagat tgctctgtca
    12961 attactcatt tcaataattc gctctaggac caccgatata tttaggacga gagcgaaggg
    13021 ctgtcacgtg agcttgtcca agggttttca aacgaggtct gacaggtact tggacatgtt
    13081 tgatatgcat accgattgat gtgtcaccga tatcgatacc ggcttgtcca actacgtgtt
    13141 ccacttcgac aggatcattg aactggtcaa aagcagcaac agaacaagca cctccagcat
    13201 gtagtgcagg aacaacagag acaatttcga aattttttgc ttctgccact gttcgttcta
    13261 ctactaatgc tcggttcaaa tgttcacaac cttggacagc tagcgcaatt tctttaggat
    13321 ccaacaactc ttttaacgta cggatgatcc attgaccaac ttccgcactg gaattttttc
    13381 caatatgtcc tccaacgact tcgcttgtgc tgcatcctaa aacaaaaaga tcaccttttt
    13441 tcaagtgtgc ctcagccaat atgtcattaa ctatttcagt caattggtct tttaattctt
    13501 tttctgacaa cttcattttc taaaaccttc tttttcaatt aatattgctt ccgtgcttga
    13561 atacgttgaa gacttgcagt tataggaaag gcaagtccca ttccaatcag attttggaag
    13621 atgtttcctg gtatagaagc cactccagcc ggccaatcat ataaaatcca accggcgata
    13681 ccatagccga tgatcataac taagcttcct aataaaagcc ctaatcccca catacgagta
    13741 gaagatttgc tgaaagaacc agcaacatat ccttgtaacc cgtggatcac taatgaaaaa
    13801 actgcccact gcggatatcc tgaaatcaaa tcgatcaata gaccactgga agcaccaaca
    13861 actaatccac ccgttggacc ataaagaaaa gcagcggtat agatcccggc ctcgcataat
    13921 gtcacaaatc cattcgtagc cggtacaggg atcaaaattg ttaatgaaag ggcaactgtc
    13981 aacgcaatca gcatagcata gatcgttaat tgcttagttt tcatttctca ttcctccttt
    14041 ttgaataccg ccagaccaga cacttccggt atgatcgttc atcagcacgc cattagaaat
    14101 acaatgggca acaaattttt tactttctgc tactgctttg gtaaaatctg cccctaacac
    14161 aagattcgct gttattgcag aagaaaaggc acaaccggcc ccgtttactg ttgtagtgtc
    14221 gattttttta tgagaaaata cctcataatg atcaattgaa tagagaagat ccacagcttc
    14281 atctccagcc atccttgtgc cgcctttgat cacaatgttt tttgttcctg aagcagtgag
    14341 ttctttagcg agatggatca tttctgttaa cgaagaggga agagcttgac cagaaagtaa
    14401 agcagcttct gccaaattag gcgtaatcat cgtaacaagt ggaaaaagtt cttcgatgag
    14461 ctgcgaaata tacaattgat tgatcacagc ttcagtttct ttgaatgcca aaacagggtc
    14521 taaaatgatt gggatatttt gttttgtttt tatgaagtct cgaacaacca tgattgcttc
    14581 acttgaattc aataacccaa ttttgatacc atctaatgaa gtcattttat caatcgaaac
    14641 taactgtcgt ttaagcaatt ccaaagatat agattccagc ataaatcgct cattaatatc
    14701 tagtgcccct atagaagtca aggcgcttag tccgaataga ccaaaattct caaaagtttt
    14761 taaatctgtt tggattcctc caccagcaaa tggatcagaa ccgccaattg tcaagattct
    14821 tttcataaca ttttcctcca tgaataagag tataacgaaa ttttcaaaaa agaaaaacag
    14881 ccaattggca tatttttatc tcatccaatt taaaaaaaca aaaagaaaag aaagaccaaa
    14941 atggatcttt ctttccttaa atagttagga cttttgtgat tttaattctt tctcgatttg
    15001 aagccgttgc actttcattg tagctgttcg cgggattgca tcaaaatcac ggatgatagg
    15061 gacgttcaag cgaggaagtt catggacttg ctcccaccat cgattccaat ccatttcttt
    15121 atcaggagct aaagcaataa tcggttgtgg agaatgattt ttatccctta caatcactac
    15181 ttctgccaaa aaatctaacg aatctaataa aaagtcttca atagctaatg tactattgat
    15241 cgtttcgatc aaatccactt gccgatcttt caaaaatagg tggccatgct tatccataaa
    15301 accgtaatct ccgctatccc accaatcgcc ataaacattt tcctggaacc gtgcatcttc
    15361 cttgtaataa gtcaaagccc gtcctttaga cagtaattgt atatgaccat ctgtgttttc
    15421 aggtaggaca ttcccaactg aatctgtaat ccgagcgctt gtcatgtctt ccagtccaac
    15481 gcccatatct cttgcatcac ttgttttcaa actctctagt gtatgagcct taagaatcat
    15541 tggtccgcat tcactttggc catatacttg aagaaatacc gcatcctgtg ccatcgacgt
    15601 tcgcaaaaat gtcttcattg tctggttgtt tatagcatca aaagtggagt gatagtatcg
    15661 gatacccgaa aaagcttctg gatgctcctt agcagtaaaa cgccactgaa caaagttatt
    15721 cggatgggtc tctagtgcaa ttggccggtg agcttccaac atatgaacaa cttttgaact
    15781 ttgtgcgttt gctaaaggca tcataggaaa tcccatggac atcaaagaag agataccaat
    15841 attgaatctg gaatgcactg gagagatgtg aaaagcgatt aatttctttt ccgaaatttt
    15901 tgtgaaaact gttttttgcc atttcgtccg ccatcccata gaatgagcag aatgacaaat
    15961 aagtttaggg ataccagttg taccagaagt atgggtcata taagaaattt catttttgtc
    16021 taaaaatgat tgagaaacag acaaagcagg ttgttccatt aaataaagaa taggaa
//
LOCUS       DAZAFF010000066        15909 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000066 DAZAFF010000000
VERSION     DAZAFF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.066"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            225..893
                     /locus_tag="ACG9BB_001992"
     CDS             225..893
                     /locus_tag="ACG9BB_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFN2837924.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1093..1323)
                     /locus_tag="ACG9BB_001993"
     CDS             complement(1093..1323)
                     /locus_tag="ACG9BB_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837925.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1320..1478)
                     /locus_tag="ACG9BB_001994"
     CDS             complement(1320..1478)
                     /locus_tag="ACG9BB_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837926.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKMKKKHA
                     NSLFLNNK"
     gene            complement(1450..2832)
                     /locus_tag="ACG9BB_001995"
     CDS             complement(1450..2832)
                     /locus_tag="ACG9BB_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN2837927.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADQANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2988..4175)
                     /locus_tag="ACG9BB_001996"
     CDS             complement(2988..4175)
                     /locus_tag="ACG9BB_001996"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFN2837928.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4182..5213)
                     /locus_tag="ACG9BB_001997"
     CDS             complement(4182..5213)
                     /locus_tag="ACG9BB_001997"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFN2837929.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5259..6683)
                     /locus_tag="ACG9BB_001998"
     CDS             complement(5259..6683)
                     /locus_tag="ACG9BB_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFN2837930.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6858..7763
                     /locus_tag="ACG9BB_001999"
     CDS             6858..7763
                     /locus_tag="ACG9BB_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFN2837931.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7854..8531
                     /locus_tag="ACG9BB_002000"
     CDS             7854..8531
                     /locus_tag="ACG9BB_002000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFN2837932.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8528..8698
                     /locus_tag="ACG9BB_002001"
     CDS             8528..8698
                     /locus_tag="ACG9BB_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837933.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8812..9708
                     /locus_tag="ACG9BB_002002"
     CDS             8812..9708
                     /locus_tag="ACG9BB_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN2837934.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9763..11133)
                     /locus_tag="ACG9BB_002003"
     CDS             complement(9763..11133)
                     /locus_tag="ACG9BB_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN2837935.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11371..12117
                     /locus_tag="ACG9BB_002004"
     CDS             11371..12117
                     /locus_tag="ACG9BB_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFN2837936.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12114..12953
                     /locus_tag="ACG9BB_002005"
     CDS             12114..12953
                     /locus_tag="ACG9BB_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFN2837937.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12954..13903
                     /locus_tag="ACG9BB_002006"
                     /pseudo
     CDS             12954..13903
                     /locus_tag="ACG9BB_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            14044..14796
                     /locus_tag="ACG9BB_002007"
     CDS             14044..14796
                     /locus_tag="ACG9BB_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837938.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14815..>15909
                     /gene="queG"
                     /locus_tag="ACG9BB_002008"
     CDS             14815..>15909
                     /gene="queG"
                     /locus_tag="ACG9BB_002008"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFN2837939.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEE"
BASE COUNT         5155 a         2985 c         3209 g         4560 t
ORIGIN      
        1 gtccaatttc gtaaaacaac cgtaacggtt gtgtaaaaat atcattttag agcataaaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acagtgtcac
      121 agtccttttt ttagtaaaag gatacatttc ttccttgtat attccatgcg tgttcgtgag
      181 aaagcatata taatttaatt aacagatagt tagggggaga aaaaatgatt ttttcaatta
      241 caatgaaact ggtgttcggg ttgatcggtt tattattagt ggtacgtttg cttgggaaga
      301 aatccatgtc agaaatcaca ccgtttgatt tagtctatac gcttgtttta ggtgggatct
      361 tagaagaatc aacctatgat gacaatgtcc atgtagggca tgtcctattt gccattgcgc
      421 tttgggcagt aatgatttat ggtatcgaac gtatcgtcca aaaaaatgaa aaggtcaatc
      481 gttgggtcaa aggcgagcca tctgttctaa tcaaagacgg cgtcatcaac atgacagagt
      541 tgacaaataa ccatatcgag atggagcagt tgcgagcaat cttaagacag caagaatgct
      601 tttttcttga aaatgccaaa catgttatcc tagagaatgc cggtcaaatg agtgtattga
      661 aaaaatcaga tgaagacaaa gctttatcga tcctgcttgt agatgaaggg caaattcagc
      721 ataaagtgct tcagtccaat caattgactg aagcatggct tatggagaac ttgaagaaag
      781 aaggctatgc agatgtaaaa cagctgattt atgttgaatg gtcagaagaa aaagggctct
      841 acatactaaa taaagaagac gtcatgcatg aatcttaccg aatagatgga tagatagaca
      901 gaggttgtga aatcagcgcc ttggcttgtt actcaaaact aagcgacttt ttcacaacct
      961 ctgatatcta atatacgttg ttccagaagt tgcttcttcg aaaaataagc caaaattcca
     1021 caaaaattta agaagcaatt ttcgaaaaat cctttcttat tttttgaagt tgatcacttc
     1081 tgtcacaacc tctcatgtca caaaccattt ttttgtttcc tttaactcaa tataccgtac
     1141 gtcttcccac aatatcggct gatggtcaat cataaagtaa tcttcatagg cttcaccaat
     1201 gaagtgccct tcgacctcat ccaagagtct tccaaattca tcttttaaat tgagctgaat
     1261 gattactgct ttacgagata aaaaggcttc tgtcagtata ctatcgattt cttgcaatgt
     1321 catttgttgt ttaggaagag gctgtttgcg tgcttcttct tcattttttt gataccgttg
     1381 actagttctt ccatcgcaaa agcagtcccc cattttaatc caaatggccg atctcgatac
     1441 tcgttatagt caatgaattt ttttgctgtc cgtctcatct tttccacctt ctaatccact
     1501 cataccacct gcatgcccgc caacgagaga actacgtgcg atggcgcgtc ctccttgcaa
     1561 gagactatgc gcatggatga ttgattgaaa gccgtatttt tttctaatcg tatcgacaac
     1621 aaaatcaact ttcgcttcat ttatctgctt ttcaggatct tcaaaaaggt taagctgtaa
     1681 acctgtcgcg tatacgaggt cggaacagtt cacgcctaca cgccgaatat cctggtagtg
     1741 gtaatgctgg tggaaaatgg tcaataaagc atctgcaaga tctctgctag catttgtcgc
     1801 ttcgattttc atttgctggt gaaacccggt cttgccattt ggatcaagat agcctaaaga
     1861 gtatcctacc cataagctga ctacttgtgt ttttgctgca gatcgtctca agcgagtgcc
     1921 gacttggtcg gccatttctt tgataacgat ctcgatttct tctcgtcttg tataatctct
     1981 atctaggatt tgactatttc cgatactttt tgattttggt gtatattctt gtcccaaaaa
     2041 acttcgatcg atgccccaag catgggcata aagctgagta ccgatgaccc ctagcttgct
     2101 tttcaagtga taataatccg cgtgagctaa atcataaaca gaatgtatac ccatttgttt
     2161 cagacgtttt gctgttcgtt tgccgattcc ccaaaagtca gtgatatctg agattttcca
     2221 taacttttct ggtacatctt cataacgcca ttcagcaaac atttgctttc cgtcttttga
     2281 gccattgtct aaggctagtt tagcaagtag cgggttatcg ccaattccta tagtcgtgta
     2341 gattccggtc tgctcaaaca catctttatg gatcagttta gccatgtcgt acgcatttgt
     2401 tttaccaaag agtgccatgc tatttgttac atcaaggaag ctttcatcga cggaatagac
     2461 atgatggttc gcttggtctg catatttttt atagatagaa ttgatttctc tgttcttttc
     2521 catataatag gccatccttg gggcaacaat atataaatca cttggatagg ggaagggaag
     2581 atctctggca cggctgatat tggtgatgcc gtaagctttt tttgctgcag gactggaagc
     2641 caagattaat ccgctacccc gtgtttctgt cgaatcggac ggataggaca tgactactaa
     2701 tttagttgta aggggatcaa gatccttttc aacgcactct acggaggcgt aaaaagattt
     2761 gcagtcaata cataaaatat ctcttgaagg ttctttttca taaacaaata ccggattttg
     2821 attcgcccac ataacaatca cctcttcaac acaattagaa cacacgttct ctttccgtgt
     2881 caataaaaaa cagcaatttt caaatacgag caaaaggctc acatttggaa attgctgaga
     2941 ggttattttt atctatttgt ttttagctga gatcgttgat ttttttatca aacatgaaca
     3001 gcttctggat cgatctttgt tgtcccattt ccaatgtgtg caacataaaa ttccggagca
     3061 tagccgacaa ctttcatgta ttcatcatag acattgtttt tgaaggcagg tatattttct
     3121 tctttgacaa gagcaatcgc acagccacca aatccagctc ctgtcattcg tgcacctaag
     3181 acaccttctt gtttttgagc agcatctact agtgtatcta attcgatccc agttacttca
     3241 taatcatagc gaagagagtg gtgagaatca tttaacagtt gaccaaatgc ttctaagttc
     3301 ccagcttcta attcagcttt tgccttcaaa gtccgctgat tttcagaaac ggcatggcga
     3361 gctctgcgta tcaatgtatc atcgccgatt tggtcaatag ttgattcaaa ttcttcttca
     3421 tctaagtctc ctaaagaaga gataggcaaa gttttttgca aacgtgccaa ggcttcttcg
     3481 cattcgctac ggcgttcatt gtattttgaa tctgccaatt cccgccgctt attcgtattc
     3541 atgatgacga ttgcataacc atctagtttc actggaacca tttcgtattt cagcgtattt
     3601 gtatctaata agattgcatg atctagctca ccgaagccga tggcaaactg atccatgata
     3661 cctgaattga caccgatgaa ttcattttct acttttttac ctgtttggac tagttccaag
     3721 cgatctgttt ttaaatcaaa cagatcttct aatacaacac ctactaataa ttctaatgaa
     3781 gcgctactgg aaagtcctgc accattaggg attgttcctt ccactaataa ctcaaaccca
     3841 tgatcgattg agtgtccagc ttcttttaat ttaaggacca ttccttttac gtaatttgcc
     3901 cagtttgctt ttttatcata ttctagctca tctaaagtaa aactaatgac accgatatcg
     3961 tcaaagtttg tggaataaac taatatttta ttatcgtctc gaggtgctgc tacaccttgc
     4021 gttccataag tgatggaagc ggggaagaca tggcctccat tgtaatctgt gtgttcacca
     4081 atcaaattga tgcgtcctgg tgagaagtat tgtgtagttt ttttaggtcc gaatgtttcg
     4141 gcaaattttg tgtttaagct tcttttgatt tccatgatga gttattcctt tcttgttttg
     4201 attttgaatt ctgttactgt ttcaaatact gatccggctt ttaaatgaat cgaaccaaat
     4261 gctgagaagc gctctgcacc tggagctgtt tgagtctcaa atgtgatgcc accgtgatgt
     4321 gccagttttc ggttgcgcat ttctggtgtt tctgtgccga aatttgcagt gaaaataacc
     4381 acactagaag catctgtagc aatatctact tggattcttt tatcggggct agtcaagcga
     4441 gcggcttctt tacccaagcc ggtttctttt aagaaaaatg gatgatcgat tccgtcaaca
     4501 agattttttt gatccaaatc actagcaaaa acggacgcta attttttagg tatttgaaaa
     4561 tcgaaagcac ttcctgcagc attttctttt acccctatag gaatactatc tgttcgcaaa
     4621 ggagcataag cttcgctatt gagccaaagc tcatgttgat cgattgactg agaagcatct
     4681 cccgtcaagt tgaagtaaac atgatttgtc ggattgaata aagtatcttg gtcactggtg
     4741 ccgcgagagg tcactcgcca gatattatct tttgtcaaag tatagcggat ttccacatcc
     4801 attgtacctg gaaaaccatg ttctccgtcg ggacttgtag tagtaaaaat gacagaagct
     4861 tcattttcgc cattcaaaat gacataagac cattttttta attcaaatcc aggagcacca
     4921 ccatgcagat tatgtccagt ttttggatct gtggctaatt ggtaagtttt tccattgaga
     4981 gaaaagcggc cattttcgat tctccctgcg gtgcgaccga tagaagcacc gatatacgga
     5041 tctttttcga tatattcttc agcggtgtca aatcccaaaa ctaattctcg atcatttgat
     5101 tttaaactga cgattctagc acctaaatca gagatagaaa gtgctagctt gtttttgttc
     5161 tccaaggtta tcaagtgata accttggcca aaatcttttt ctgtaatctt cattttcaag
     5221 atcctttctt aatgggaagg aactactgca tcgtgaagtt aatcttccat tgtatcagct
     5281 ggagccaatt tgttttccaa tttctcaacg atttgtgcat gttttttctc tgtcaatgta
     5341 atcttccaag caaaaataat agcggagaaa atcatcaaag cagcaggagc atagaatgca
     5401 taagctttga aagtcgttac gccatgagca gtgatatcag cagcagtagc attaccagtc
     5461 atcccagaag cgatagcgac aaaccctacg atcccgttag acaaagcacc agcgatttta
     5521 tctagtaaag ggcgaattga tagagtaaca gcttcgttac ggataccatt tttccattga
     5581 ccatattcaa cagagtctgt gatcgtcatc aaagcggata agaaaatcaa ttgttgaggg
     5641 aagtagaaca atactagccc gatgataacg attggcaaac ttgaacctgc gatcgtaaag
     5701 aaaaagtaag aagaaatcat cattgcgatc cctccaaggt aaacgtagcg acgagagatg
     5761 acgcgtgtaa gcattgggaa tagtggaaca gctactaaac caagtaaggc agcaactaga
     5821 ccaacgatcc aaaattcagt tggttggcca agtacatatt tgaagaagta gaataatacg
     5881 gcagttgttg ctgcattggc aatcgcataa gcgatataag caagggcaag ccacatcaac
     5941 tgatcatttt gagcaactgc tttgaacaca tctttgatcc cagctttttc attttgctga
     6001 cggatcgcag tatcgacttc ttttgtgccc aaggcagtga taatcgcagt accgcctgaa
     6061 ataacagcag caatcacacc gaaccagaac cagcctgaag cgccttcaat atgtttatga
     6121 gtaacaagat aggtaaagaa agtaacgata gggacaacga ctagagtagt accatttgca
     6181 cctagagaag agccgaaacg agcaaacgta gcaaattttt cacgttcctt tgtttccgta
     6241 ctcaaagctg gaatcattga ccaaaaagcg atatccttaa atgaataaaa actatccaat
     6301 aagatgaaaa cgattgcaaa taatatgata aataaagtcg tattatgagt agctaagcca
     6361 aagaaattgg taaagattgc taccaagagt acggaactaa caacgccgcc gataactaac
     6421 catggtttga atttaccgta ttttgtacga gtattatcga tgatgccacc aatcaaagga
     6481 tcaaaaataa tttcaactaa gcggatacct acaactaagc ttgtaactgt agcaatcatt
     6541 tttgcattcg ttgccttatc tgttcctgcg aacatctggc ttgtcacgaa taccataaaa
     6601 taagtgctta atgtaacata gaatgtgtcg tgtccaaaag cgcctaaagc gtaggacaca
     6661 cgttggatca ttttgctttt cataatctgt attcctctcc tgttcaatat ataatactaa
     6721 gtaaaccggt tacaacgaat aacaaagatc ttttaatgac ttaaacttaa caagaaatcg
     6781 cttccttttt aatatatttc tgtgactttt atttaacttt ttgttgcatt atactaaagg
     6841 agtaaggagg gatagacgtg gagcaatcga tttttttaaa aaaggaacat caacaagtag
     6901 aaaaagaatt gtattttgat ttttgtggtt tctctaaaac gatgccgttt cattcttttg
     6961 gtcctgctat tcgcaatgat tacatcctgc atgtcgtgat ggaaggcaaa ggatcttatc
     7021 atgtgaaaaa tcaacaattc caattgaaaa aaggtgatct gttcttgatt cgtccaggag
     7081 actcgacgtt ttacttatca gatggagaag atccgtggat gtattgctgg ctttcatttg
     7141 gtgggagtgc tgcagaagaa atcattcggc agtcgctttt cagagaagac caatacacga
     7201 tggtctcttc tgacatacaa aaatatatcg acattatttt agaatgcatg aaagatacgg
     7261 aagaaacctt ggaaaatgaa ttacagttaa cagcattaac ctaccgtttt ttatcagtcc
     7321 ttttaaaaga tggcggaaag gtcagcggtg gtgtacaaaa ggttcattcc tctttagctg
     7381 ttgaaacagt caaatacatc gaggcacatt atgctgaaaa attgtctgtt gaggaaatcg
     7441 cccagcattt agcggtcaat cggagccatt tgtcgcgagt attcaaaaat catatgggta
     7501 ccagtatcaa ggaatatttg atcggggtac ggatcaatcg aggggcgttt cttctttctc
     7561 tgacggatga atccgtggaa agtattgcct atcaagtagg gttcaatagt ctagtcgtat
     7621 ttagccgaat gttcaaaaag agtaccggag aaacggctac gaactatcgt aaacgtatga
     7681 aaaacgaaaa ttttaagaat attgctttgg ataccttgaa aaaacagttg gaaaaacaaa
     7741 ccgcagtttc ttggacaacc tgattagtct ggttaaaatg acaagcatcg gcaaaagaaa
     7801 cttgtggcgg ttatgctaca ctgtaaagcg aagactaaaa aggaggaaaa gggatgcgta
     7861 cacttatcat cattagccat ccaacgatcg aagaatcttt aaatcagcaa tttttcaaag
     7921 aagcttctgc tggtttacca gctacttggc atcatttgga atcaagttat ccagacggaa
     7981 aaatagatat agagactgaa ctacagctct tggcccagca tgatcggatc attttccagt
     8041 ttccgtttta ctggtatagt tctcctgctc atttaaaaat ttggcaagat aatgtcttgg
     8101 aacgagcagc acatgtatta aaagggaaag aactaggaat cgtattgaca acaggtgtgg
     8161 cggaaaaaga ataccaagct ggaggaaaag aagaatatac gatttccgaa tttttgaggc
     8221 cgtatcagcg aatcgcaaat aaattccata tgacctatct tccgccattc gttttagcac
     8281 aatttatgta tttatcccag gaaaaacgtt gggagaagct aattgcttat cagcagtatc
     8341 tctcattgga aggcaaacca tcgctgactc agcggattga ctggtttatt caaagagtac
     8401 aggaaaatca gaaaatgcag gaagaagaat ctgaaaaaca agcatatatc gtagaagcac
     8461 tgactgaggc aaaagagcag atcgaagact tgacctttac actgcaagaa atgaaaggaa
     8521 cttcattatg acaaatgatt ggctgatcca acaattagat gaattaagcg aaaaatcaga
     8581 atatcaagac cgtgcgctct tatatgaaat aaagaaactg atggctgaga tagacgaacg
     8641 aacaatgcag ctgcaaggag aaatcgacgg acaatcttgg aatcatctca actggtgaaa
     8701 ttttcttata aataacaaag ataaaaatgt gcacagcgac aattatttgc tacaataaga
     8761 atatgaagcg tttgataacg ctattataca taaaaattgg tggtgaaaaa aatgtatgat
     8821 gaaaatgagt ggggagaaaa tgttcggatg acccgaagaa gacagcaaaa acaggcagct
     8881 cgtaagcgga tgatcatgat tggggcagca gttgccgtgt ttttgatcat agcaggagga
     8941 gttgcgggtc tatattttca tggaaaaaat tcgtcagaag cgaaaagtac gaccataacg
     9001 gaaacaacga caaagaaaac agagacgaaa caaacaagtg agtcatcaac taaaacaaaa
     9061 gagacacaaa cttctgaaga aaaaacagca aatggtttgc cagagcaact tcaagcaatc
     9121 gtaaatgatt attcgaaaaa gatcgaagaa aagacgcctg cattgattga agaataccag
     9181 acagaaattc agggaaatca agagggaata gctggtttat cagcagttgc taaccaaaaa
     9241 gcacgtgagc ttcaagcaat ctccgacgaa gggatcagaa aattacgtgc agcgtatcag
     9301 gcagcagaaa ataaagacgg tgtagactta gatactttga tcaatcaatt atcggctaat
     9361 tatacgaatc atgtcgctaa gatttccgat atttatttgc agactagcgc ctctcttcaa
     9421 gcagagtcaa caagcacaca agacacaaca tcttctgatt ctgagacagc agagtcggta
     9481 gaagcttctg aaatgcaagc tcgaacaaca caagacacaa cagattcctc tgaaactaat
     9541 accaacagcg cgacttcaca ggctgcgaca accgtagttt tggaaggaga aggtccaaat
     9601 caaatagccg aacgaactgg cgtaccagta gaaacgatct tgtcgctgaa tgggatgagc
     9661 atggagaatt atttcctgac tcccggtgag acgctaaaat tgaattgata aaagcaataa
     9721 aaaagaacga agcttccact aaggtgaagc ttcgttcttt ttttattttc cggttctttt
     9781 ttcgcgtaca aagtaatgga tcgtcatgta aagcaagaaa acgagtccaa taatcaatga
     9841 gattctcgtt tcaggattga taaacatgaa aatcaatacg atagctaaaa aagcaagtgt
     9901 cacataattc gtataaggag atagtggcat tttaaaagga tgatcatgca ttttatctgc
     9961 gtgcttcttt ttgaagcgaa tctcgctgat caaaatcact acccaaggaa ccataccagg
    10021 caaaatactt gagctgtata cgaaaacgaa aatattttct gactggttga tgaacattgg
    10081 tagaagataa tttaagatca gaccaattaa gattccacct gagattgcta cgacaggata
    10141 aaaaggcgca ccgtgacgag atagtttcag aaatttttta gacatctttc catttatggc
    10201 aagtgtatag accatacgac ttgcgctgaa aatccctgaa ttgcaacctg ataatgccgc
    10261 tgtaatcacg acaaaattaa tcagtcctgc agcaaatgta atgccgactt tagaaaaagt
    10321 ttgtacaaaa ggtgaaccaa tctcactcaa ttgattccac ggatagatac tcacaatgat
    10381 aaaaatagca ccaatataaa aaatcaagat tcgaccgata gtggactgga cagcttcaat
    10441 gatcgtcttt ttaggattct ctgcttcacc agcagtcatt ccgatcagtt cgattccttg
    10501 ataggaagca acaacgatcg ataatgcaaa gaaaaatcct ttgacgcctc cagtgaagaa
    10561 tccaccattt ttccaaagat ttgaaatccc tactgcctga ccatgattac caaatccaaa
    10621 taagattacc ccaagacctg caatgatcat caaaatgatc gttatgactt tgatgatcga
    10681 aaaccaaaat tctaattcgc caaacgcctt gacagaaata agatttgcca gcataagaaa
    10741 caagacgaca atgattccgg gaataattgc cggcaaatca ggccaccaat aattcatata
    10801 agatcctaca gcaatcactt cacttattcc tacgacaata tattgaaaaa tattactcca
    10861 agcggtcaag tagccagcca caggatggat gtactcgctc gcgtaatttg caaaagaacc
    10921 agtcgaagga tcaacgtaaa gcatttcgcc taaagcacgc ataatcaaat acaaaaagaa
    10981 tcctgcaatc gcataggctc ccatcactga gggccctgtc caacgtatgg ttgaggaggc
    11041 acccataaac agaccgacac cgatcgttcc acctagtgcg atcatttgga tatgacgggc
    11101 actaagactt cgagttaact tttcttcttc catacaaaaa ctcctttcgt tgttgagtca
    11161 acaagaaaaa ataatcaaac gtttcaacac atgtttttca ttattaggtt agccttattg
    11221 aactcttgat gtgattctac agaaaatttt cataaaatgc gaggaaaatt cgaaaaaaac
    11281 tttttcatca ttctacttga aaatgctttc cattttagtt aaactatatt taaataaaaa
    11341 gttaggtaaa cctaaaaagg agtgtaagat ttggaacgta cggccattgt aattcaagac
    11401 ctttcagccg cttatcaagg gaaaactgtt ttgaaaacta tcaacttgac gataaatcca
    11461 caaaaaatta ccggtattat cggaccaaac ggtgcaggaa aatcaacatt tatgaaatct
    11521 ttgctagagt tgataccagc tactactggt aaagtcacct tttctggaca gtcggtaaac
    11581 tcagtccgta aaaaaatagc ctacgtggaa cagcgaagtg aattggatct ttcctttcca
    11641 attgatgtat taggcgttgt acttttagga acatatccat ctttacgaat tggacaaaga
    11701 cctgggaaaa aagaaaaaga acgtgcaaga caagctttga aaaaagtagg gatggaagaa
    11761 tatgccaaaa gacagatcag cgaactatcg ggtggacagc ttcagagagt ttttattgca
    11821 agagctctag cccaaggagc agaatggatc tttttagatg aaccattcgt agggattgat
    11881 gcgttaagtg aacgaaagat ctttgacatc ttgcaggaat tgaagaattc aggaaaaacg
    11941 attttgatcg tccatcatga tcttcataaa gtagaagaat atttcgatga ggttattctt
    12001 ttaaataaac agctgatcgc ttccggtcca gtacaagaag tctttacatc gaagaacctt
    12061 caattggcct atggtgagat catcagacat ttagtaaaag gaggagagaa aaaatgatcc
    12121 aatcctttat tgatggatta atgaattatc aattcctgca atacgctttg gtcacttcca
    12181 tgcttgtagg acttgcttct ggagtcatcg gttcttttat tattttacga gggatgtctt
    12241 tgatgggaga cgctatctct cacgcagttc ttccaggtgt agccatttct tatatgtttg
    12301 gttttagtta catctttgga gccactgctt ttgggatgct aacagcagca gtgattggtt
    12361 tcgtgactca gcggagccga ttgaaaaatg atacagcgat tggaattgtc tttagctcct
    12421 tttttgcatt agggattatc ttgatttcat atgcccagag tgcgacagat ttgtatcata
    12481 ttttatttgg gaatgtctta gctgttaggg aaagcgattt gttgctgaca gcactcgtta
    12541 gtggaatcgt cttgatattt gtctttttct tttataaaga attgaagatc acatcattcg
    12601 atccgacaat ggcaaaagct tatgggctga acacatcgct gatccattat cttttgatgt
    12661 tctttttgac attagtggct gtagtcagtt tacagacagt aggaacaatc ttggtgattg
    12721 ccatgttgat cacaccagcc gccacggctt acttgctaac gaaccattta ctgaaaatga
    12781 tcattacagc tgcaggaatc ggtatgctaa gtgcagttgt cggtgtgttt ttcagttata
    12841 gttacaattg gccatcagga gctacgatcg tgttagcatg taccgcattt tttatccttg
    12901 cttttttatt ttctccaaca aaaggaattt tatttcagaa aggaagcaga tagatgaaaa
    12961 caaaaaaatc actcttttta atcttggcag ttagtttctt ggtattagcc ggttgcggaa
    13021 agcaggcttc tgaccaagcg gatgaaggaa gtaaagaaaa gttgtcagtc gtggctacca
    13081 attcgatctt ggcggacatg gcaaaagagg tcggaacaga tcaaatagat atccacagta
    13141 tcgtaccagt cggaacagat ccgcatgaat atgaagtact accagaagac atcaaaaagg
    13201 caagcgatgc agatgttatt ttatacaacg gtttgaatct tgaaacaggt aacagctggt
    13261 tcgataactt gatggaaacg gctaaaaaag aagaaggaaa agattatttt gcagttagca
    13321 aaaatgtaga acctctttat ttaactagcg gtgaagaaca tacaaaagca gatccccatg
    13381 cttggctaga cctatctaac ggaataaaat atgtggagga aatcgcacgt atattctctg
    13441 aaaaagatgc agaaaatgcg acactctata aaaaaatgca gaagcatatg tggaaaaact
    13501 aaaagaatta gatacacaag cgaaggaaag ttttgcttct atcgaagaga acaaaaaatt
    13561 attagtaaca agtgaagggg cattcaagta tttttcacga gcatatgatt tgccagcagc
    13621 ttatatatgg gagatcaata cagaaagtca aggtacgcct gatcaaatga aagcgatcat
    13681 tgatcagata agagcaaaag aagtaccggt tttattcgtg gaaaccagtg tagactcaag
    13741 aagcatggaa cgggtagcaa aagaaacggg tttgaaaatc tatgataaac tgttcactga
    13801 ttccatagca aaagaaggag aacaaggaga ttcttactat cagatgatga aatggaatat
    13861 tgaaacgatc catgaaggtt tgtcacaaac aaaagaaagc tgaaaagtgc aaggtctagc
    13921 ttgaggagca gaccttgcat tttttataag agatatgaaa aaaaattatg ctgtttttcg
    13981 tcgtacagat accttttttg aagaaattcc gttatagtta gagtaaggaa ggaaggtgtg
    14041 gctatgaatt ttacacgtaa agataaatat acgattaaag aagaagtctt tcgattgatc
    14101 agccagcagg aaaataccga caaggatcaa gaccttcaat ttcttgaagc agaattagct
    14161 aaattattca tagcaaatca aaatgagaca acagaaccag atgaagagct gtacataggc
    14221 aaatggttgt ttgatgaatt tcagccttta gatgaaaata ccggtatcgg ttattttgtg
    14281 ttcctttctc cagtcggctt cacacctggc gtattagtta aacaaaacga cgaaataacc
    14341 gtcaaagcat ggcctgcatt attttcagtc atcaaagcag aaggagtaat caaaaagatc
    14401 cagtcggcct attataaaag tcaatttatt gccaacagca aattaatgaa tatgttttat
    14461 aatcgtctag tagaaattgc taggcctggg aaaatcccgc aaacattcac gtacacacag
    14521 caaaaaaatc tctatgaaga agtattgtat tcccgcgatc tgtctgagtt ttcgccatgg
    14581 gaagaggtaa ggctccatga actgttaagt acaaaaccag tttcattgaa tttttttaat
    14641 gcattagata acaaaagaaa attaatttca acaaattatt gggaagtatt tgctgcaaga
    14701 aaaaaatgga tggaagaaaa gaagattgcc gatcagcaga tccagtttgt ccagctgaaa
    14761 aaagaaaaac tatattatgg gttatacagc cgttaacagc aagcgaggaa gaccatgaca
    14821 ttaaaagaag aaatcataca agaaagcaaa cgattaggaa ttgacaaaat cgggttcacc
    14881 acggcggaac cttttgattc attaaaagaa tcgttggaag aacaaaaagc agctggccat
    14941 acttctggct ttgaacatcc aaatatcgat gagcgtcttt atccagaaat gacatttgat
    15001 cagccgaaat cgatcatttc cattgcactt gcgtatccta ctaggatcca tgaagaagtg
    15061 cctagggatg aaaaacgcgg gcagtttgct cgtgcttctt gggggatcga ttaccatgat
    15121 atactcagag accgtttgaa ccgattgatc gaattcatcc aaacaagagc agaaaaatgg
    15181 caaaaagaag aggaatggcg tctagctccg caagtagata ctggagaact gatcgatgtc
    15241 gctgtagcac agcgagctgg tttaggattt attggacgaa acggattgtt gatcactgaa
    15301 gagttcggtt catttgtcta tcttggtgag atcgttacta atattgtatt tgagcctgat
    15361 aaaccaggag aatttggttg cggtgactgt atgcgctgcg tgactgcttg tccgacaaaa
    15421 gcattgcttg gtgacggaag aatgaatgcc cagcgatgtt tgtcttatca aactcagaca
    15481 aaagggatga tgccggaaga atatcgtaaa aaaatgcgaa atgtcattta tggatgtgat
    15541 atctgccagt tagtctgccc ttacaatcaa ggaaaagatt ttcattttca tgaagagatg
    15601 gaaccaaaag tagacgaaat atatccaaaa ttaaagccaa tgctttcgat gtctaataaa
    15661 gacttcaaac agcaatttgg gcatttagct ggttcatggc gtgggaagaa acctttgcag
    15721 agaaacgcat tgattgcttt agctaatcta ggtgatcgaa cggcattgcc tgagatttca
    15781 gaatgtttaa cagatgtccg ccctgtcatc agaggaacag cagcttgggc tattggacgt
    15841 ctaggaacaa aagaaccgga aaaatggctt gatgtattag atgaaatatt agctaaagaa
    15901 acagaagaa
//
LOCUS       DAZAFF010000067        15659 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000067 DAZAFF010000000
VERSION     DAZAFF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.067"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG9BB_002009"
     CDS             <1..1087
                     /locus_tag="ACG9BB_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2837940.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="ACG9BB_002010"
     CDS             complement(1305..2447)
                     /locus_tag="ACG9BB_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2837941.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="ACG9BB_002011"
     CDS             complement(2452..2637)
                     /locus_tag="ACG9BB_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN2837942.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="ACG9BB_002012"
     CDS             complement(2991..3236)
                     /locus_tag="ACG9BB_002012"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2837943.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="ACG9BB_002013"
     CDS             complement(3230..3631)
                     /locus_tag="ACG9BB_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN2837944.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="ACG9BB_002014"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="ACG9BB_002014"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFN2837945.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="ACG9BB_002015"
     CDS             complement(5649..7415)
                     /locus_tag="ACG9BB_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFN2837946.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7428..8222)
                     /locus_tag="ACG9BB_002016"
     CDS             complement(7428..8222)
                     /locus_tag="ACG9BB_002016"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN2837947.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8232..9608)
                     /locus_tag="ACG9BB_002017"
     CDS             complement(8232..9608)
                     /locus_tag="ACG9BB_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2837948.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANTTDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9728..10375)
                     /locus_tag="ACG9BB_002018"
     CDS             complement(9728..10375)
                     /locus_tag="ACG9BB_002018"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFN2837949.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="ACG9BB_002019"
     CDS             complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="ACG9BB_002019"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFN2837950.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="ACG9BB_002020"
     CDS             complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="ACG9BB_002020"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN2837951.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13042..14673)
                     /locus_tag="ACG9BB_002021"
     CDS             complement(13042..14673)
                     /locus_tag="ACG9BB_002021"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFN2837952.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14977..15648
                     /locus_tag="ACG9BB_002022"
     CDS             14977..15648
                     /locus_tag="ACG9BB_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2837953.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         5074 a         3059 c         2113 g         5413 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gctaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagtgat aattccttta
     6421 cgatagcctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat
     6721 ccagcttcta ttaagcctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta atagaggaat tttactttct gtttgtaaga tatagttttg atcatatacc
     7201 tcatcagcag ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaaatc tacttttata gtcattttct ttctcctcta cttattagta
     7441 agatttttcc aaaaagtatc tatatatttt tgttcaatat ctacataatc tcccgataaa
     7501 catttaaata aaacatcagt tcttaagttt tcccatgatt ctttttcttt attgaccaaa
     7561 ataggataaa agaaaacttt atttttattt gcagcttcta gatcaccagg agaatcacca
     7621 atcattaata tcttatccaa atcacaacca ttttcaatta cacttcgaat aatatcttct
     7681 ttttttcctt tatcttgaca acacagttca tcaactaatg ataataattc atgtctttcc
     7741 cattcttctt gaacagcttc cttattagct gagcttacaa taattatctt cccatatttt
     7801 ttcagagctt ttatagtatc tctagcactt aaaaagggtt tatctaatcc ttttgcttga
     7861 ctaatctgtt gattgacttc ttccgaccat tttaaagctt ttaataaatc ctctgactga
     7921 gaatattcta tttccctctt caaagatgga ttagacaatt cttttgttag agatacccag
     7981 tgctttaatc gattaatcga tgttacatca tttttttcta atgttttaac taaacctaaa
     8041 aatctgttta ttccacgagt ctctgaatat aaattaatat tattccaatc ctgtaggaat
     8101 ttttctctat gatgaacatc aaaaatctct gcagcaattg gtccgaaaaa gagattgtgt
     8161 ttataagtca tcgtatccat aacacagcca tctgaatcaa tacaaaatat ccaattattc
     8221 atatttctct cctatttatg tttttgtttt aaaagctcgc aattttctaa gactttatct
     8281 ttagataatg gataaaggaa aaataaaatt aatcctacaa tcagaaaaca aattgatgga
     8341 attaatgttg aagcgttata taacttttct aaaacatctc ccgtttgtac atttgctaaa
     8401 gagtcatagc ctataataga aagagtccaa cctccgacgc ctccagctag agcttgacct
     8461 atttttctag caaaagaata taccgcatat acggtcccat cttctctttg atcagacttt
     8521 acttcttgat catcaataac atcagtagta tttgcccaaa taacagtatt aaacacaccc
     8581 aaacccatat agccaaccga actaagaatt aagaatacat acatattact agcttttatc
     8641 aaaaataaga tcaaatatac tattgctgaa aagaacattc ctactgatgc cagttctttt
     8701 tttccatatt ttttacttaa aaacaaagtt aaaggcgaaa caactattaa aaccaaaata
     8761 ggattgataa aattcattaa aactattccc tgtgcatcat tatagtaatc aggaaataca
     8821 taattattca tactcataat taataattga gcaaggagta agaaaatagc cgcaccaatt
     8881 atagaaagta atgccctatt tttaactaac ccttttaaag attttaacaa tgaaactttg
     8941 tattgagatt ggtcaattct tactcgttca gtagttagtt tataacatat taaataacaa
     9001 ataagtgcac atatcgaaaa tattcccgca acaattgtaa aagttgatcc accacgaact
     9061 atttgagcgc ctgaatcatc tcttgtataa ataaataatg gtgttaatac accaatgacc
     9121 aaacttgcta aagtagctcc aacatttcgg aatgtcgata gcattgttct ttcttctgta
     9181 atagaagtaa tagctgatgc cattgatcca taaggaatat ttatagatgt gtaaaaaata
     9241 ctcccccata atatatacgt aacatacatg tatactacct taacgctcat agaagctgaa
     9301 atcatagaag tttgatacat taaaaaactt gctaaagcga ctggaccact cattcttaaa
     9361 atccacggtt taaacttacc ctgcttagtt gctttcgatc tatcaacaat acgccccata
     9421 gttacatcag taactgcatc gataaatctt gctactaaaa ataatgttcc aacggcagct
     9481 ccgcttatcc ccaatacttt tgtataaaag atcattaaaa aagaactagc aaaaatgaaa
     9541 gtaaaatcgt ttccaaaatc tccaaacata tatcctattt tgtctcttat accaaatttt
     9601 ctttccatac aattctctcc cttctgttgt taagcgtttt cattctgatg aaagtacgta
     9661 ctatactctg aagcgtaaag agtcagtatt tattttttac atttcagagt attatttgtt
     9721 ttttctccta ataattcttt atctcataaa acttatctat atatttacta gctactcgac
     9781 ttacttcttc aaaatcacct ttttcaacag aagccaataa attcccacct acaccaactg
     9841 caacaactcc tacttcaaac caatcttcta agttctctat acttactccg ccagtaggca
     9901 ttatatttac gtgtggtaat ggagcttgat aggcttttac aatggatgga ccaaaagcag
     9961 aacctgggaa tagtttaatg atatctacgc cagcagtaag tgcctccttt atctcagtaa
    10021 ttgtcataca acctggtaaa taaggaattt gatataaatt acaaatctca gctgtttgtc
    10081 gatcaaaaga tggactaacg atatattctg ccccagccat aattgcgata cgagcagtaa
    10141 acgcatctaa aaccgtaccg gctccgatta ttacatcttt ttgatcagca tattttttag
    10201 caatttcttt gattatttct tcagcattag gaactgtaaa agttagttca atccctttaa
    10261 ttccacccgc aataattgaa tttattgctt taatggcctc atccttattt ttcccacgta
    10321 caaccgcaat gactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta
    10381 tctccttcct ttatggatac atactttgtc tagctgtact aacttacaaa gaataaattt
    10441 aagcttttat ttcaagtatt attttctact tccgatttca aaaaaattaa aataattata
    10501 cgcattacga taacaaatat tctccactat ctgactaaga tacatttcat catctggaat
    10561 tcggcctgct ttaacccact cgcttagtaa ttgacataat actcgacgaa aatactcatg
    10621 gcgaggatag cttagaaaac ttcgagaatc agtcaacata cctgtaaaat tcgacaacaa
    10681 actttgttgt gccatcgtag tcaactgttg acgcattcct tcagctgtat cattgaacca
    10741 ccaagcacat cccaattgga ttttttgaat cataccccct tggaagcttt gcattgctgt
    10801 tgctagtgga agccaatcag ttggatttaa tgaatataaa attgtttttg gtaagcaccc
    10861 actcttttca gcggtatcga acaatgctac aacattttgt gtcaaactag cttgatcccc
    10921 catcgaatca ccaccgacat ctactccaat ttgttccttt aatatacttg aattatttct
    10981 taaagcattt aaatgcattt gcatcgtcca accatatctt gtatactcag acatcaaatg
    11041 taattgaata cctgtaacaa attgtgcaat ttctgataaa tggaaatccg tttttcctgc
    11101 taaagctttt attaacagat tgtcgagtat tttttcatct gctatttcat aaaaataagt
    11161 attcaaaccg tgatctgcta atcgacagcc aatttgatga aaatgatcca ctcgttggct
    11221 caatgcttgc ttcaacccat taaagtcttt gattggtata gccgctgttt gactaagttt
    11281 taaaatatag ttcgaaaagc ctgatgattt gatactccag accttatctg gacggaaagt
    11341 aggtaatacc ttgaaagaat tatcctgttt agaaagctca gtatgatacc acaagtcatc
    11401 tgtaggatca tccgttgtac agatggcttt cacatccatc atctccaaca attctttcgg
    11461 gcgataggaa tccgtttgaa ttttttcgtt agcacgtcgc caaatttctg gtgcattctt
    11521 ctcagaaatt gtcaaatcaa tatcaaagta acgttgtaat tccaaatgag accattcaaa
    11581 aataggatta cctggcgcct tctccactgt ttttacaaaa gctaaaaatt tttcataatc
    11641 atcacctgga ccactgatca actcttcttc tataccatta gctcgcatca atcgccattt
    11701 ataatgatct ccagctcctt tatcatttaa ccatacctga gtcaaattct tgtattgctt
    11761 attttctagc aaatatgctg ggtctaaatg acaatgatag tcgataattg gttggtgttt
    11821 tgcaaattta tgaaaaagtc ttttagctat ttcattctct aataaaaagt tcttatctaa
    11881 aaacatattt aaacactcct ttccttccgt tagtttttag aagagaaaat ttttcctcta
    11941 aataaactcc cttatataat tatttactcg cccccttttc aagcgcttcc caaataccat
    12001 ttaaataaac tgcacctaga gctcggtcat ataatccata accaggtcta ccggtttctc
    12061 cccaaatcat ccgaccatga tctggacgaa tatagccatt aaatccatta tcgtacattg
    12121 tttttaatat cttatacata tctagagaac catattctga tttatgcggc gcttcgtaaa
    12181 aatcgagtcc attgtcactt aaatatttga tattacgaac atgtgcaaaa ggaatccgat
    12241 cccgttgaca aaattcagat agaatttcat agacatcatt atctggtcgt tctgcaatac
    12301 ttccagtaca taatgtaata ccattagaag gagaatccac tacattacac aaccaatcta
    12361 aatcgtcacg atttttaaca atacgaggta atccgaaaat ggaatatggt ggatcatctg
    12421 ggtgaactgc cattttaatc ccaacttctt cacatgtcgg gataattgct ttgagaaaat
    12481 atgcaaagtt ctctcgtaat ttctcttcat caacttcttt ataggcttct aataattgct
    12541 taatttgtga taaccgatga ggttcccaac ccggcaattc aaaattatta gtagaatttt
    12601 ctacttctct aataatttcc tccggatcag tagggatatt ttttgaaata taggctaacg
    12661 tagaagaccc atcatctaaa cgataatcca aatccgattt tacccaatca aatactggca
    12721 taaaattata acagataacc tcaatcccaa attctgccaa attgcgaatc gtctgtttat
    12781 aattctcaat gtattgatct cgtgttggta aaccaatttt gatatcatca tgaacattaa
    12841 cactctcaat aactttcaag gtaagtccat tggcttcgac ttcatctttt aaagatttaa
    12901 ttcgttctct cggccaaacc tctccggctg gtatatccat taatgttcca acaattcctt
    12961 tcattgctgg gatctgacga atttcttgta gtgagatttt ttcttcattt gccccatacc
    13021 atctaaaagt catttccata gttactttcc tccaacttct gttatatatt tattgattgt
    13081 tttacgaact gcttgaggac ctactagcat ttcgcaaaag attttttcag ttttctttcc
    13141 taccccaact tgatataaat ctaaaccaaa gatttcttta ttttctaaaa tttcctgtac
    13201 cgcacaatga atctttgaag gatcttgttc acctaatttc aatactgata atttatcctg
    13261 caactctacc aataacggat caggacttgg cacaaaattt tccccttgat catcgactgc
    13321 cagtaaataa cgtaaccaag cagcaatagc gataggaata aatgttagat tttctgcatt
    13381 tccgtctttt tcgacatact ttttaattgt ttcaccatag cgaatcgcta ctttttgaga
    13441 agtatctgtt gcaattcgtt gtggtgtatc tggaatatag ggattaggca aacgtttatt
    13501 aattacttca tcaataaatt gttttggatc aataatgcct ggattttcaa ctactggtaa
    13561 accttctaaa taacctattc ctttgattaa accaaccaaa tcatcatcct gcatctctgc
    13621 tgaaattgat tgatatccta gcagacaacc tgttactgcc aaagcagtat gaagtggatt
    13681 caagcttgtc gtaaccttca tgatgtctgc tttatcaacc gtctctctat ctgtcataat
    13741 cacaccagcc ttttccaatt taggccggcc atttggaaaa ctatcttcaa taactaaata
    13801 atgaactacc tcagtattag tgaaagcagc aatattggta tgtttttttg tgtgaatgat
    13861 atctaaatcc tcgagcccca tcatcaatag atcttccttt acattctctg aaggatttgg
    13921 cgtaattcga tcaatcatac tccaaggaaa agtcactttt tctttatcct ttaaataagc
    13981 aataaatttt tcagaaacat atccttcttt ttgccaagct tctgcaatcg taaaaatact
    14041 atcttgaaaa cgttgaccat tctgtgagaa attatcagta gaaaccatcg caatcggcaa
    14101 ctctccagca ttaaatctgc tatataacaa ggatgttaca atactcatcg tatgttgagg
    14161 attgctaggt ccagatttga tatcagcttt gactaccgaa aagaattgcc ctttagaatc
    14221 ttttaaactg taaccttttt cagtaatagt aaacgttgta aactgtaatt taggatcttc
    14281 aaaatattta accactcttt gatagtccgc tatattcttc ggattgcaat ataaactatc
    14341 agccgttact gccaaaagtc tttttttgag ttcaccatct ccactcataa caatttccaa
    14401 aatgtcattg ttatagtcat gataagcttt ttccactact tcttcatcaa atgtttcaca
    14461 cacaactact cctgtttcta attcattttg atctgctaag cattgagcaa tttctgcatg
    14521 aaatcctcga taaagatttc ctccaccaaa atgaagccat actggatgtt gctggccttc
    14581 ttgcttaact tctttgacat caaaacacgg cagtagaatc tgcatttcat tgaattcttc
    14641 gatagaattt tgccaatttt taatcgaaac cataattaac ctcctcaaac tgatatgtta
    14701 gttttgatac attttatatt ttaaaaccca ttgtaaccgt tgtcaatata aaaagggaat
    14761 aaacttgctt ttttctacca tatcagaatg attgtatttt ttattcagaa aaataaaata
    14821 ctccttcttt ttggactgat tttatcgaaa cttatttttt ctttttttca tgtattcttt
    14881 tatttttata actagtgtat tacttcaaaa tttgatatta tatggcatga acagaattaa
    14941 aatatcagtt aaaataaaag ttaaggagca ttgtgtatga caattaaaac tagtctccaa
    15001 aatcaagctt accaatctat tcgccgacaa attatctatg ctgacttaga acctggaaaa
    15061 aaagtatcag aaaaaaaatt agaagaaaca ctttctattg gtcgtacacc aatacgtgaa
    15121 gcactgattc aactccgaca acaaggtctt gttgatacaa ttcctcaaag cggaaattat
    15181 atctcattaa ttgatttaga ttcggccaaa aatgctcgat ttgtcagaga acatttggaa
    15241 cgacaaatta tgttagagtg ttgtgccaaa atgaataacc aaaaaaaacg gattttagaa
    15301 actataattt cagaacaaga aaaagctgtt agtcaaaaaa atgctcgtag tttttttcat
    15361 aatgataatt tattccataa agcttgtttt gaaattgcag agcgagaaga agtatggcaa
    15421 tggttagaaa ataacaatac acacttagaa cgatttcgat ggctacgagt aaaaacggaa
    15481 ggattaaagt gggaaaaaat tatggaacaa cattatcaac tatttgaggc attaacaaaa
    15541 caagatccag aagaagctaa ctttctaaca tcgttgcatc ttcatttaat gcttaatgag
    15601 caaaatattg ttttagctac tcatccaaaa tatttcaaaa atatataact tttaactct
//
LOCUS       DAZAFF010000068        14961 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000068 DAZAFF010000000
VERSION     DAZAFF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.068"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..2377
                     /locus_tag="ACG9BB_002023"
     CDS             5..2377
                     /locus_tag="ACG9BB_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFN2837954.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..3898
                     /locus_tag="ACG9BB_002024"
     CDS             2438..3898
                     /locus_tag="ACG9BB_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFN2837955.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3909..6113
                     /gene="topB"
                     /locus_tag="ACG9BB_002025"
     CDS             3909..6113
                     /gene="topB"
                     /locus_tag="ACG9BB_002025"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN2837956.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6129..6278
                     /locus_tag="ACG9BB_002026"
     CDS             6129..6278
                     /locus_tag="ACG9BB_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837957.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6353..6946
                     /locus_tag="ACG9BB_002027"
     CDS             6353..6946
                     /locus_tag="ACG9BB_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFN2837958.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6959..7858
                     /locus_tag="ACG9BB_002028"
     CDS             6959..7858
                     /locus_tag="ACG9BB_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFN2837959.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7861..8358
                     /gene="ssb"
                     /locus_tag="ACG9BB_002029"
     CDS             7861..8358
                     /gene="ssb"
                     /locus_tag="ACG9BB_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN2837960.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8724..8984
                     /locus_tag="ACG9BB_002030"
     CDS             8724..8984
                     /locus_tag="ACG9BB_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837961.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9429..9635
                     /locus_tag="ACG9BB_002031"
     CDS             9429..9635
                     /locus_tag="ACG9BB_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFN2837962.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9656..9886
                     /locus_tag="ACG9BB_002032"
     CDS             9656..9886
                     /locus_tag="ACG9BB_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837963.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9902..10339
                     /locus_tag="ACG9BB_002033"
     CDS             9902..10339
                     /locus_tag="ACG9BB_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837964.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10332..11039
                     /locus_tag="ACG9BB_002034"
     CDS             10332..11039
                     /locus_tag="ACG9BB_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837965.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11203..11403
                     /locus_tag="ACG9BB_002035"
     CDS             11203..11403
                     /locus_tag="ACG9BB_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837966.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11419..11598
                     /locus_tag="ACG9BB_002036"
     CDS             11419..11598
                     /locus_tag="ACG9BB_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837967.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11923..12186
                     /locus_tag="ACG9BB_002037"
     CDS             11923..12186
                     /locus_tag="ACG9BB_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFN2837968.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12180..12530
                     /locus_tag="ACG9BB_002038"
     CDS             12180..12530
                     /locus_tag="ACG9BB_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN2837969.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12554..13168)
                     /locus_tag="ACG9BB_002039"
     CDS             complement(12554..13168)
                     /locus_tag="ACG9BB_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2837970.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13482..13904
                     /locus_tag="ACG9BB_002040"
     CDS             13482..13904
                     /locus_tag="ACG9BB_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837971.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13914..14117
                     /locus_tag="ACG9BB_002041"
     CDS             13914..14117
                     /locus_tag="ACG9BB_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837972.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14328..14912
                     /locus_tag="ACG9BB_002042"
     CDS             14328..14912
                     /locus_tag="ACG9BB_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2837973.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5512 a         2429 c         3035 g         3985 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagatt atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ccctaagcaa caatttaaag cagccactga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtggaa aatcttcatt ttgaaacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gcagaaaaag aagggttgat
     2881 tgccgaacta aagccaaaat atgaacggca aaatttctta gttcgagacg atcgtttaga
     2941 tcatgcggtc gcctttttat ggaaagatcc acagacaaaa gagacagtag gggcgagtta
     3001 tcaaggaacg ttcattgatt atgaacgttt tggtgagaga ggcacatata agcatataga
     3061 taaaaattcg acagcgaatc atggctttaa cctaaagatc ggtgacccta agcagataaa
     3121 attttttgag agtagtattg atttacttag ctatgctgca ctgaatcgtg atcagttaaa
     3181 cgatacgtgg ttagtctcta tggaagggtt aaaacaccat gtgatcagtc attattttgg
     3241 tgaggcagta tctgaattga gaaagaaaca agcctttcct caatccatcg agatttgtgt
     3301 ggataatgac cgtgcaggac atattttcta cgaaaaagaa caattaatgg gcgcagtcga
     3361 tccatttacg aatcagaaag tacgttgtga acgaggaatc gctaatgatt ggcaagtgcc
     3421 aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa gaaaaggtca caccagaagc
     3481 tattatggcc atacacaaga cagaaaacaa tctgcaatta acgaaccaac tggtttccgc
     3541 tcacaaagta aatgcgtttt ttggccagca gctttctgtt aacgattcaa tagaagccat
     3601 taatttgaaa gatatttgta gagaagtcgc aaaagaatta aaagtttgtg aacgcgtgga
     3661 tggtacgtac gattttgatc gtttttatca agaaaaaggg gatattaatg cgcagattct
     3721 tttctcttat aaagcagaac aatactacaa aggctataaa aatcatgaac atgaatttgt
     3781 acctgaagta aaaaaggact ggaacgatca attgaagcat gagatccagc aacaagaaat
     3841 cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga caacaagaaa gggagtgaaa
     3901 tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc acaagcgaaa gcttatgccg
     3961 atagcttctc taaagccact cgaaaagacg ggtattttga aatccaagat cgattgtttc
     4021 ctggtgaaac tgtgatcacg tatggttttg gtcatttagt cgaattagat tcgcccgata
     4081 tgtacgatga aaattggaaa caatggtcat tagaacattt gcctattttc cctacacatt
     4141 atcactatca tgttcctaaa gataagaaaa agcaattcaa ggtggtgaaa cagcaacttc
     4201 aatcggccga caccattatt attgcgacag atagcgatcg tgaaggggaa ctgatcgctt
     4261 ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac ctttaaaaga ctttggatca
     4321 actccttaga aaaagaagcc atttatcagg gattccagca gttacgagac gcaaaagaga
     4381 cctatccaaa atttgaagaa gcacaagcac gtcagatcgc cgattggtta attgggatga
     4441 acggtagccc actgtatagt ttactcttgc agcaaaaagg cataccagga agcttttcct
     4501 taggcagagt ccaaacccca acgctttaca tgatttatca attgcaggaa aagattagaa
     4561 atttcaagaa agaaccttat tttgaaggaa aatccgaagt aatcgctcaa aatggcgcat
     4621 ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca agaagcattt gaagattatt
     4681 taaaagaaaa gggggttcaa ttgggaaaac agccaggcat gattcgccaa gttgaaaccg
     4741 agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc cagccttcag tctaaaatga
     4801 atcagctcat gaaagctagt gccaaagaca ctttggaagc catgcaaggc ttgtatgaag
     4861 ggaaatatct aagctatccg cgaacggata ccccttacat tacggaaggg gaatatgcgt
     4921 atctgttgga tcacttagac gaatacaagc attttttgag ggctgaagaa atttcgactc
     4981 ctattcatac accgaatagt aggtacgtga acaacaagaa agtccaggaa cattacgcaa
     5041 tcattcctac aaaaacagtt atgacagaag cgacgtatga aagtctgaca cctttacaac
     5101 aagcgattta cgagcaagtt ttaaaaacga cagtggcgat gtttgcggaa aaatatacct
     5161 atgaagaaac aacgatcttg acgcaagtcc agcagcttca gttaaaggca atcggaaaag
     5221 taccgataga tctaggttgg aagaagttat ttggcaaaga gagcgacgga aaagagaagg
     5281 aagaagagcc gctacttcct aaagtgacaa aaggagaaat ggtgacggta gatctacaag
     5341 tccttgaaaa agaaaccaag ccaccacaac cttataccga aggtacgttg atcactgcga
     5401 tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca atccatattg aaagaagtgg
     5461 aaggaatcgg tacagaagcg acacgggcga atattattga aaccttgaaa caaaaggaat
     5521 atatcaaggt agagaaaaat aagcttgtgg tgacgaataa aggggttttg ctttgccaag
     5581 ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac cgccaaatgg gaaagctact
     5641 tgttaaagat tggagaacga aaaggaacac aaaccacttt tctagccaat atccaaaagt
     5701 ttgtctctca cttattggaa gtcgtcccag gacaaatcca atccattgat tttggatcga
     5761 cgttacaaga agtgaaagca gccagtgaaa agcaagaagc cgcgcgtcat ttaggaattt
     5821 gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa ggtggcagct tgtacgtctg
     5881 aagcctgtga ttttaaatta tggacaacga ttgccaaaaa gaaactcaca gcgacacagc
     5941 tgaaggagat tattcaaaat agccgaacaa gccaaccagt gaaagggtta aaaggtcaga
     6001 agggttcgtt tgaagcaacg attgttttaa aagaagactt cactacaggc tttgaatttt
     6061 ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg cacgacaaaa taatggaggg
     6121 ttaatcttat gcgtaataca acacacaaac aacaagaaat cgaaaatcaa aaagccaaaa
     6181 ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag acttcagcaa ttaaagaacc
     6241 agcaacggat tcaaaaagaa atcgaattag atcgatagac attaaccttg ttacaaatac
     6301 actatcatga tatatttaat gtagtgtatt tgtaacaaaa ggggggaagt aaatgcaaga
     6361 gaaactgaaa gaattgttgg ctattcgcga tggaaattta tctatgaagg aagccagaaa
     6421 attaggaatc gcagcaacca ctgtacaaag attggtgaat caaggaaaat tgatcaaagt
     6481 tgatcgtggt tattatgtag aggatggtca tggaatagat gatttatttt ggcttcagca
     6541 acgtttttcc aggggaatct tctctcatga aacgacttta gatatttacc agctttctac
     6601 gaatatgccg aaattcattc acttaacttt tccccatgga tataacgttg atcgttcaat
     6661 tacaaaagag cagcagttac aatttcattt cgtgaagaaa gaagtttatg aactaggaaa
     6721 agtagaggga acttcctttc aaggaaatcc tattactatg tatgataaag agcgtacact
     6781 ttgtgatatt tggaatccga gatatgaaat tgaatatgaa atgaaattag aagcattaaa
     6841 agagtacatg gaagacccat tgagagatcc aagtaaatta agagattaca tgacaaaact
     6901 acatgtagaa aaagaaatga aataccacat gcaaagctta tattgatgga ggaaaaagat
     6961 gacaccgaca caatttgcag caaaaacaag aaatttagca aagaggacag gattaaatgc
     7021 acagttagta caacggcatt ttatgatgga tcgtttaatt gaacaaattt ctgaatctaa
     7081 gtataaaaat gatttcattt tgaaaggtgg ctttttatta ggatcaaaat atggaatcga
     7141 tagaaggacg acaattgata tcgacacgac atttagaaat agcaaattaa ctgaagaaaa
     7201 attgaagagt atttttaatg atttaactag gaaacccacg aaagaaggta ttgttttctc
     7261 tattcaagga atgaaggaaa caagagaagc cgatttttat cctggatttc aaattaaatt
     7321 ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg gacgtgacaa caggagattc
     7381 tatttatcct gaagtagata cacactatca ccaattaatg tttgaagata aaaaggtgga
     7441 gattccagct tatccaacag cacaaattat tgcagaaaag ctaagtgcta cgttcagctt
     7501 tggcacagat aattcgagag caaaagatta ctacgactta tttaccattc cgaaactaga
     7561 aaatatcgat acgaaagagt tatacaaaag tgttcgaaat acatttacaa aacgtgggga
     7621 tacaaaaccg ttgtcctttt attatgaaag agaaatggat aagatcatag gaaatgacta
     7681 tttggaaaag cagtgggaaa aatatcgctc agttaacacg tttgcgaatt ccatttcatt
     7741 tacagataca gtcaacgaaa ttgatcacct aatgaaaagt gtgatcaaaa ttgaaaatca
     7801 agaaagaaaa cggcagttta aaagatcaca acaaatgaat caagaaatgg agagataaag
     7861 atgatcaata atgtaacttt agtaggacgc ttaaccaaag atccagattt acgttatacc
     7921 gcgagtggta ctgcagtagc gacttttacc ctagctgtca accgaaattt cacgaatcaa
     7981 aacggcaatc gagaagctga ttttattaac tgtgtgattt ggcgcaaacc agcagaaaca
     8041 atggtgacat tagctaaaaa aggtagcttg attggtgtag ttgggcggat tcaaacgcgg
     8101 acttatgaca accagcaagg acaacgtgtg tatgtgacag aagttgtggc cgataatttc
     8161 caactattag aaagcaaagc agcgacagaa agccgtgcgc acgctgatca atcgtccacc
     8221 agtccaagca ccaccacttt tgaacaacgg gatacagcga cgcctaataa caatggattg
     8281 aacgctttac agaatccatt tgggaatcca tttggtggac aatcaatcga tattagcgat
     8341 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     8401 aatttagtca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     8461 tggttttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     8521 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     8581 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     8641 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     8701 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     8761 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     8821 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     8881 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     8941 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaaaaaag aagagttgca
     9001 atcctaatgt aggactgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     9061 ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc tctctcactc caaccgccac
     9121 cgccgttcga gaagaaagga agtgaacgtt gcacgttcac taagataaag ggtcttgcaa
     9181 agcaagacaa aatagaaaaa acttcctctc accaaaaggt tcgttcctgt tcgttatctc
     9241 tttattgatt tgccagaccg gcaacaagtt gccttttatc accaaaaagc gacaaggaca
     9301 agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc
     9361 aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag
     9421 aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc cttcagcgaa ttattcccaa
     9481 actatcatgc accacatact gtcgaatgcg attgtggaca aagagcgaaa tgtatcaaaa
     9541 gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata caccttgtct tatggcgatt
     9601 atattttgat tgatgagaaa ggaaagaaac gctgatgaaa acataacata aattattgac
     9661 tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc atttctgaaa aagtagcaga
     9721 agctttactt attccgcggt actggacgat agaaacccct ttagtagagg atgcaccgac
     9781 tatgctattt gaagacctag tgaagattgc aggatatgaa ctgcattatc aagaaaacat
     9841 ggaaacttat gaaaacgaat attggttaac caaattagag aactaaaaag gagaaaaaaa
     9901 tatggctcaa aattactatg acgaatttgt caaattacct ttggataaaa tggcacaaaa
     9961 aatggaggat atgacattcc tataccatga gacacgtgtt ccaaaaaaac attataagga
    10021 aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt gtcgaaatga atctcattgc
    10081 gacgtactat cgaacactgg aagaactgaa aaaacaaaat gcaaaatggt ttttccaagc
    10141 cttactatgc ttggaagtag gtgtcaaacc aagcactatt aaaccttctg aatatcaagc
    10201 tttagaacta acgtatggga aatttgtcga aacgaaaaaa gccaagactg tttcaagtga
    10261 atggttagat tattttgaaa atatcaataa gtatggcgcc tattacacca tgaaaaagga
    10321 ggacaacgaa aatgaataag gtgatttatc aactaacaac ggaagatgtg tcacactttt
    10381 taacaaacaa agaacatttg aatgaagtaa tcagacggat tcaaaaagac tttagttgta
    10441 tggaagaaat gaagtacttt attcaaagag tcgaagaaca attgtatttg gaaagtgaaa
    10501 cgagtcaaac gaaggtcaca gtcagcaata attatttaga cgtgtataaa catcgtcctg
    10561 aaacggtaaa aaaaggaacg gagcttctac gactagaaac agaaaaatac atcatgaatg
    10621 tttacctaga aaggagtgaa ctggtcgaaa actttgtcag ctgcacgatt tgcaggaaac
    10681 actattactc agaatcagaa tattattcaa tttttgagag catggaatta atggaagaag
    10741 aaatggaaga gctaaacagc aaaaatgcga cgatagacga ttattatctt gtatttacta
    10801 gatttttgac gtgtggaacg gaatctatcg tgttaatggg aatggattat gaactcgctg
    10861 tacccgaagc atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt
    10921 acacgacttt aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa
    10981 atgaacttga ccgtaaacga atgccatatc aacgatttga aaggaaaaaa aggcaataat
    11041 catttatacc gaaaagtgag aatgtgtgaa ataagaacaa aaaccttgaa attaagggaa
    11101 tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc gcttaggtgg tgcgaaataa
    11161 gcgggcaaag cccgataacc taagagaaag aaggagatcc atatgaataa aatcattcct
    11221 ctgttgttcg tactgtgtat tggcgagata cttatgcgtc atggtgcagc tgtacattta
    11281 aacttcgtag tattcttagg aatactagta ttttgtttga ttttttggcg ctttgtgtta
    11341 gcccttgtga gtctactatt caagttgatt gcagccatat ttttcatagg gttatttcga
    11401 tgaaaggaga gagtactaat gccaattgca ataggaaata aacggttacc cgtgacatta
    11461 gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga agtacggaaa aagtgaatct
    11521 aagattatgt gtattgcgtt agatctattg atcgcgcaag aaaaagcagg atttgatata
    11581 ccagcactaa aaaagtgacg tcacttttca tttgaagaaa caaaagtgat agcacttaat
    11641 tgttcctact atttagaata ttttctatgt cctttttatt ccattttgta tcaataacta
    11701 tgaaatcttt aaagtatcca gatcaatttt tttcttacct gattctaaag attttgtttt
    11761 cttaaacata ttatgatttc ctttcgacta ttttcatata aatgttaaca tagtttatgg
    11821 attcatggaa tgttaatcta atttaataga aaaggataat taagtatata cgttttttct
    11881 tatgtatgtt atactatgta tatacagaaa gaggtgtcta ctatggaaat gttacctata
    11941 aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt atattatgga atatcttgga
    12001 attcatacag aagacaaagt gaagataata caagaagaag taaacggtca gaagcggcta
    12061 attatcgaag cggctaatat agaaaatgaa ttaacaattg aacagttatt tgaaaattac
    12121 actgatgaaa gaaatcatgt gacgattcaa aatttaggtg aagcagtagg taatgaaaaa
    12181 tggtgagaaa acctagacaa ggcgatattc tattattgaa tacagcacct cgatctggac
    12241 acgaacagac aggtaaaaga ccttatattg ttttaagtca tgatattatt gcggattata
    12301 gtaacgttgt tacagttgct ccaatttcta gtacaactag aaactatccg ctatatgtaa
    12361 atataaactc agaatatgaa atgaagacaa caggaaaagt attattagat caactaacga
    12421 caattgatta tgaagccaga gagtgtattt ttttggaaaa agcgcatgat agtttagttg
    12481 aggagttatt gatgaaagtt agaacagttt ttcaaaaagt aagcaaataa aatagaaaca
    12541 gcagcatgat caactattcg tgattgacca tgctgtcttt ttttattcta tatacggttt
    12601 gtctcgtaac attgtagtct tttgcaatct tagcgatcgc aacacctttt tttagatcct
    12661 caacgatgtt tttataaaca agacgacgtt ggggatcttt tgcattggca ctgtatagct
    12721 taggacgtcc tttataaaca ccgttcgctt ttgcaatctt aatgccttgt gcctgacgtc
    12781 gtttagattc ggtacgttct tgttcagcga tcatggccaa aatttgaatg atcaaatctt
    12841 taatgaattt atccaggaga ggattgccaa ttgcttcggc catgatcggt agacttgtga
    12901 tgatcagctg cacatttttc ttcttcaagt aattgactga agcgatgatc tcgtcgtaat
    12961 ttcttccaag tcgatcaata gcttccacaa taaaaatatc ttgatctcgg acgaaatcca
    13021 gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc agattgtttc tcaatgaaaa
    13081 ttctctctgc accgaatttt ttcatttctt caatctgccg ttgttcattt tgatcgatgg
    13141 atgagacacg tatgtatgca actctcaaaa tttttaccta ctttctgcaa ctatttaaaa
    13201 aacaccctaa tcatacacga gaaaaccaga tttttcaatg tcgcggtcgt gtctagttag
    13261 ggtatacctt atatagacaa aaactaatga tggaggaaat agtatgtgtc acttttcttt
    13321 ttaaatggca atattttttg taaataaatg tcaatcaaga aaatttaaac gattatgaca
    13381 ttaaaaatat attttatgaa ttttctagta tataacttga tttatctgtt aagatttatt
    13441 aagaaaacgt tttcttattt ttgatttcga ggtgatagac aatgaaaaat aagtttattt
    13501 tatctttagg tattttaact attggaatta gtgtaactgg aactacaatt cctacatttg
    13561 ctgatagtac aaatgaacca actactgtag tagataataa ccaagaatta actcctttag
    13621 ataggaacgg aatagccatt ggtaacccaa ctaacgactt aactgttact gaaaattatt
    13681 taatagtttc tccattactt gctcgagcag cttctaaaac gatacgagtg acaggaacga
    13741 tgatttttga taaagttggt tggccttcta gacctcctca atcacggaat tggaaagaaa
    13801 aaagaaatgg ttattggtac tatggaaaat tgtatttaca aagttataaa tcagctggaa
    13861 acaattggat tgcgacttat tcaggaacct taactagaaa gtagggattt tttatgccag
    13921 aatttgtgaa cacaccaaat ttttatcaag atagcgatat tctaagtaaa attaatttac
    13981 cgaaagaaga atcagcttta caagttcagc gaactttaca ggagacacaa aaaattcaaa
    14041 aatcaatttc taatctagaa gattctgaag aaaaaagaaa aaaaagagac caaaaaacat
    14101 atcaaaaaga aaaataatga ttttaaaaaa agagtgtcta gtcgacattc tttttttcta
    14161 attgaaagtg ttcataaaat cacccatttc tggacacttt ctttttttgc tttatttttc
    14221 aaaaaaacaa ttcatttcat gttcataaac ccataacaat atctatgttc agttaaacta
    14281 tatggataaa gttatgttac aataaaatta atgaaggagt gatttttttg aaaataggat
    14341 acgcccgggt atctactggt ttacaaaatt tggatttaca agaagacagc ctaaataaat
    14401 ttggttgtga aaaaattttt actgatcata tgagtggggc aaaaagtaat cgaccaggat
    14461 tagaaatggc cattgatttt gttcgctctg gcgatacact tgtcgtttgg cgcttagatc
    14521 gtttaggtag aaatatggaa gatttgattt caatcgtcaa tcgactgaat gagcgtggtg
    14581 tgagttttca tagtattcaa gaaaatatta cgatggacaa atcaagttct acaggacagc
    14641 tcatgtttca tttgtttgcg gcatttgcag aatttgagcg taatttgatc ctggaacgat
    14701 ccgcagcagg acgagcagct gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat
    14761 tgtctgaaca agatttggaa ttactgaaga ctttagtgga tagcggtaca cctattaaga
    14821 ctattgcgga acggtggaac gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg
    14881 aggagaaaaa tggaaagaca aattctcatt agtttttgtc atttaatgct gaaaattttt
    14941 gccatttatc tagaaaagtg a
//
LOCUS       DAZAFF010000069        14438 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000069 DAZAFF010000000
VERSION     DAZAFF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.069"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..310)
                     /locus_tag="ACG9BB_002043"
     CDS             complement(<1..310)
                     /locus_tag="ACG9BB_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837974.1"
                     /translation="MKNLTQNLLKKDNSSILKIELKKENNKSMKISKKAFLLVVLILL
                     STLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSSIFEGPINYTTFNNYGDGLNYFYPFL
                     T"
     gene            372..2009
                     /locus_tag="ACG9BB_002044"
     CDS             372..2009
                     /locus_tag="ACG9BB_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837975.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2154..2894)
                     /gene="truA"
                     /locus_tag="ACG9BB_002045"
     CDS             complement(2154..2894)
                     /gene="truA"
                     /locus_tag="ACG9BB_002045"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFN2837976.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3085..3720)
                     /locus_tag="ACG9BB_002046"
     CDS             complement(3085..3720)
                     /locus_tag="ACG9BB_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFN2837977.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3867..5087)
                     /gene="sstT"
                     /locus_tag="ACG9BB_002047"
     CDS             complement(3867..5087)
                     /gene="sstT"
                     /locus_tag="ACG9BB_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFN2837978.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            5306..6304
                     /locus_tag="ACG9BB_002048"
     CDS             5306..6304
                     /locus_tag="ACG9BB_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837979.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6500..8335
                     /locus_tag="ACG9BB_002049"
     CDS             6500..8335
                     /locus_tag="ACG9BB_002049"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN2837980.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8395..9111)
                     /locus_tag="ACG9BB_002050"
     CDS             complement(8395..9111)
                     /locus_tag="ACG9BB_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFN2837981.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(9338..9880)
                     /locus_tag="ACG9BB_002051"
     CDS             complement(9338..9880)
                     /locus_tag="ACG9BB_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFN2837982.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9885..10112)
                     /locus_tag="ACG9BB_002052"
     CDS             complement(9885..10112)
                     /locus_tag="ACG9BB_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFN2837983.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(10191..12071)
                     /locus_tag="ACG9BB_002053"
     CDS             complement(10191..12071)
                     /locus_tag="ACG9BB_002053"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFN2837984.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(12260..12874)
                     /locus_tag="ACG9BB_002054"
     CDS             complement(12260..12874)
                     /locus_tag="ACG9BB_002054"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2837985.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            13016..13576
                     /locus_tag="ACG9BB_002055"
     CDS             13016..13576
                     /locus_tag="ACG9BB_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2837986.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13881..14195)
                     /locus_tag="ACG9BB_002056"
                     /pseudo
     CDS             complement(13881..14195)
                     /locus_tag="ACG9BB_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         4526 a         2771 c         2254 g         4887 t
ORIGIN      
        1 ttgtaagaaa aggataaaaa tagttcagtc catctccata attgttaaat gttgtatagt
       61 tgattggccc ttcaaaaata gaagataacc ctttgattcg tgaaagatga aaagataaat
      121 cacctgttgt ataaatttct tgagcatttc tcataaaatt tactgaatat aaagtactaa
      181 gtaagataag aactactaaa agaaatgctt ttttacttat tttcatagat ttgttgttct
      241 ccttttttaa ttctattttc agtatactag aattgtcttt ttttaataag ttttgcgtta
      301 aatttttcat agcactagta tgatatacta gaaaagaatc aatttttatt acttttaaat
      361 aggagaaaaa aatgaaaaaa aacaaaaacc aaataataga tatcattttt atgttatttc
      421 taagttttgc ttacgtcatc cctctattca tgtctaaggg aatctatcat agtgtcaatc
      481 aggatactta ctttcattta tctcgaatca taggactaga taatgtatgg agttctccag
      541 taaacttcaa taacttcgat catcatggaa caatgatgaa tattttttat ccatggctta
      601 ctctttatcc agctttttta ttttataaaa tgatgggaaa tcttgtttta agttataata
      661 tctactattt ttttatcact tttttaacaa tggtcgtttc ttatttttct atgaaacaaa
      721 taaaaaacaa tcgttatatt tcgctgttat tttcgattat ttatacattt tccgcctatc
      781 gtgccattga catattccat cgtgcttcat tgggagaagc tgtcgcttta acatttttgc
      841 ctttgatact aatggggtgt tacgaaattt atattagaga ttaccaaaaa tggtactggt
      901 tgtctattgg catgacgcta gtagtgtata ctcatttact ttctgtcgct atggtttctg
      961 tatttattgg aggaacgttg tttctttctt tttatttttg ggaccaaaaa atcgctagac
     1021 tcttaagtct tttaaaagct accgtcctca cctttttttt aagtgcggga ttcttaattc
     1081 cttttattca acaatctaga gcacaagaat tgaaagtccc actgggaaaa gaattaagtg
     1141 gtatggctcc tagtgatatg ctgacacata ttttaaataa taactataac aactatacta
     1201 ttggattatt cttatttctc ggattgattg gtgcttttat ttttataaaa aaattaactg
     1261 ctgatgattt atttattttc tttcttggtg tatttgttct tttttgttca accaatttat
     1321 ttccttggca actttttaac catacaccag ttaagagtct acaatttgtc tggcggttaa
     1381 atggtttttc ttctttattt atagcctata cgatgagtat agtgatctat tatatttttt
     1441 ctactaaaaa taattcatgg aaaataatgg tctttacgtt tcttgcacta atattgcatc
     1501 tctcaggagt aagcaattct atccatgcta acaaagattc attaacactt atagcacctg
     1561 aagacgctgt tgctatagct gaaaattaca atcatactga ttatgcaaac aaagagtcaa
     1621 tctatcatcc tgatatgatt aataatgatc aatatttact aggtaaccag gaaataaatc
     1681 caacaactca ttttatgagt aacaaactta caattgaatt agataattcg aataataaaa
     1741 atactgtctt gacgacacct atttatcgtt ataaagggca agtggcttct attaatggca
     1801 aactagtaca aacaaaatta tctaagtttg gcacaactga attaactatt cctccaggta
     1861 taaataaagt cgttattact tatcagtaca caaaactagc aattgcttca agatatttat
     1921 ctatcgttac tttgatttta tttttacttt atcgatttac tttttctaaa caaaaacaac
     1981 cgagaagaga agtacaacat tctcactaga tttttattta agaaagactt tttagctgta
     2041 cacataaaca ggctaaaaag tcttttttta tatttcttta atttgcttca tacattattt
     2101 aacatcgata tttatactgt gaaacatgcg tttcatccat tatcaaagta tttttatcga
     2161 taataaactt catccaaaaa cagaccttgt gcagggacgg tttctcctgc atgttgccgc
     2221 actttgcttt caaatatttc atcgattgtt tctatcggca tggcacctgt gccgatttct
     2281 agtaatgtcc ccatgataat ccgcaccatt ttatataaga atccgtctcc gacaaaagta
     2341 aaatgaagca tttctccctt tcgttcgata gataattcat caatcgtacg cactgtggat
     2401 ttttttgatt ttttcaatgc agaaaaacca atgaaatcat gtgtacccac taatttttcg
     2461 caagctgcat tcagcttgtc catatccaac tcttgaggga aataaaagct gtggtttcgc
     2521 tcaaatgctg aaggaatcac gttgttccaa acataatagc tgtatttttt gcccaccgca
     2581 ttatatcttg aatggaagcg ctcaggcatt tcttcgattc ttttgatgac aatatcccgc
     2641 ggaaggtaac gattcataaa gtcgatcatt tctgccagtc caatttctgt gtttgtttta
     2701 aaatttgcta cttgtcctcg cgcatgggtg ccagcatctg ttcttcctga tccaataatt
     2761 tctatctctt catttgtcat ctgatgcaat actttttcga tttttccttg aatggtttta
     2821 tcgtcatctc caagtctttg ccatcctgaa tatctttttc cgtcatattc gattgtcaac
     2881 ttgatgtttc tcatatttta gtggctcctc tgtcttccat ttggtcttct ctctaccttt
     2941 ttaagattgt agcacaaaat atagctcctt gcttctctaa gacagaagct atacaagaaa
     3001 actgtggaaa tatatatttc agaaaaacaa aaaacagcca ccggatgaag tatccgctga
     3061 ctgttcaatt tgacggctca agttttactg tttgagatgc aagatactca accaggcaat
     3121 gggcacataa ttaaagaaaa aactgattct ttggcaaaaa cctattttat tcagtaatgg
     3181 ccaagaattt atagaaggaa ttcttgccag tccataaatc gccgcaaaaa gcaaactgat
     3241 cacagacagt ccaaggtata agtggcttac gtccatttgc ccattttttc gatagatcaa
     3301 gaaaagaaga actggaaaca ataaaaaacc actgtaacct aaacctgatc cgatattatg
     3361 gacccatgtc gagacattcc acgaagattc atgggtattg atactgaaca aacctgtaaa
     3421 gatacaatca cctataccat agaacgcgat ggctgctgta actagggtag ctaacacacg
     3481 agatgtacta acaaatgcag catacaatgc aggcaatgac aggatgaaaa agatccctga
     3541 aaccacagac caaataagaa acgcttttct tacaggactg gaaacctctc caaatacact
     3601 gatcacgctc ttcatttgat ctaattcagg ataatacaat cctaatacgt acggagtcaa
     3661 aaaatcactc agcacgccta ataataaaaa gaaaaaacca aatttacgta aaaaatccat
     3721 gatacgcctc caataacaat tacctatgct tttaattgtt attataaacg actttatctt
     3781 gatttaacag aaacctctat ttacacgaac atctatataa ctcattaaaa actcgaatca
     3841 ttttaaaaat actaaatatc taacgttcaa cgttcactta atccatctaa acaatctttc
     3901 aatttgattt tttccccact tcttttacgc gcgcctagct ctcctattgc agtaaacaac
     3961 agatcacttg acgaatttaa ggcagtttcc attgaatcct ggataacacc gataataaat
     4021 ccaataccga cgatttgcat cgcgacatca ttagagatac caaacaaact acatgctaat
     4081 ggaatcaata acagtgaacc gcccgcaaca cctgaagctc cacaagcagc taaagcagac
     4141 aaaatacata aaaggacttt caaaacaaaa ggtgcttcaa tccctaaaga atgtacagca
     4201 gtcaacgtca tcaaggaaat ggtaatcgcg gcaccgccca tattgatcgt agcacctaac
     4261 gggatcgata tggaataaga ctcttttgtc aatttcaatc tttcagccaa catcatgttt
     4321 atcgggatat ttgcggcaga acttcgggta aagaaagcgg gaagtgcgct ttctttcaaa
     4381 caaaagaaaa tcaatggata gggattttct ttaataaaca aaaacgtgag gaatgggtac
     4441 acaactaatg cgacaaacag catcgtaccg atcaaaacaa gtaaaagctg cagatactct
     4501 gccaatccag cgattccagt cgtagcaatt gattgataaa ccaatcctaa aatccctatc
     4561 ggagcaaatg caataatcac ttgtacgact tgtgacaacg ctatagatat ctgatccacc
     4621 aactcttttg tcgattctga acgtgctcga aaagctagtc ccaacaatac agcccagaat
     4681 aagacgccaa ggtaattcgc atcaataaga gattgaacag gattttgtgt gacattcgtc
     4741 aacatagtag ataaaacact ttccaaactt tgcggtgctt tttcagatac tgtttcttgt
     4801 aaagtaatag gaacagtaaa aattgaactt acaataactg ctacaagtgc agaaagcaat
     4861 gttcccacta aatataagat caaaataggc ttcacgaatg tcttattccc aattttatgc
     4921 ttggcaattg acgccatcgt caagaaaaaa accagaatag gtgcgattgc cttcaagcaa
     4981 ctgacaaaca acgttcccaa tattgaaata aatgtccatg tggggaaaaa gataccaaac
     5041 aatattccga ctaaaatacc aacaacaata cgttgaacta acgacatatc caaaaacttc
     5101 tctaacaaat gaccaccctt tcacaatttt aacaatcatt gtatcatttt atgcaaagaa
     5161 actaaaggta taattttcta aaaattctaa caatacaaac aaccatttct tgctaatttt
     5221 gtactttttc tgtctacacg ttgttttttg agatattcta agttaggatg ttattaatgg
     5281 gcaacttatc aggaaacgtt ttcttatgtt tttgtccaat ttttttgtga ggtgtttatt
     5341 cttgaaaaaa ataaagaagg agagttatgg cttcctgaca atctttgtcg cttgtataat
     5401 gatggtttta cagcgtatat tgatttcagc agtcactatt cctacatatg caacaaaccc
     5461 tggtcgattc tacatctata catttttaca aacttccgtt gttattggtg caaatttgat
     5521 cccactttac atgggttacc aatatcaaaa aatcgcccgt ttaaaagttt tacattattt
     5581 aagtcgtttt gcctttatat tcctaatagc cacactagtt tgcaatatct ttttttacgt
     5641 acaagctggc agcttgaata ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa
     5701 ttactttact tatgctgttt cttgtacttt gcttttttgt agtattcctt atacagttgg
     5761 atttttaaat aaattatcga aaacaacaat taaatatcta ctattatttt taacaattgg
     5821 attagttggc tgctctactc tatttaataa agacccgttt gcattacaga atggacagag
     5881 tattttatgg attttttatt tatttttaat gggttatggt ttgaaagaat ggaactggaa
     5941 agaaaaggtc cgctataagc ttcctcaagt atttatcagc ttgattgttt tattcggttt
     6001 gattattttg atgacacaaa tttcactcat catcagagga aatacagata cagcgctgag
     6061 gttttctaaa ccatattctc tcttcgcaat gtattatact ttctcttcgt ttttattttt
     6121 agaaatgcta tctgataaat taggtataaa aggaagagga aattttcttg ctgtattttt
     6181 gattgtcact caagttatat gtaatttccc tttagtttct tatcgcgtat ctacatttat
     6241 gaaacgtgaa tttccaaact cagggaaggc atggctgata aatatcgggc agttttttat
     6301 ttgatattta ttagcagtgt ccctcctagt tatcttttta ttttatatac aaaaaatccg
     6361 tttcattcat cgtttcatcg aatacttcgc ttttgattcg attgaacaat taactcagcg
     6421 ttggaaaaaa agaattcatt ggctttttgt tcataaaagt tattttttag ttgcagcatt
     6481 tttttactgc tttaccttcg tgcagatttt cgttttagaa ccaaaagaag gttggaaaga
     6541 aactatccag gttgccttaa aaatatttac tcagcgacaa gcacccatga tcctgacaat
     6601 catcattatt atgggatttt tcttcttact attgttgatt tccaatcgtt tttggtacgc
     6661 cttgactgct accttgatca ttaacttact gctcactatt tcgacagtaa tcaaaatgga
     6721 aatgcgggaa gagcctgtct ttccttcaga tttaaaaatg ctgaccggat tgtcagaact
     6781 attatcgatg gttagcccag tcttattaat cgtgggcgga ttgatccttc tcctattatt
     6841 gattactagt attattgtac aacgtagact acaaaaacag tattccttga agattcattg
     6901 gaaacgtcga tttatcagta tcgctgtatt attaggatgt ttttcgggtg tctttttcat
     6961 taatcacaaa aattcgcctt ctttcttgct gtttaattta tttaaagtaa acaagacttt
     7021 ctttaaccag caaggcgccg taaaagataa cggaccagtc attcaattcc taaacaatat
     7081 cgacattaaa atcatggaag aacctgctgg ctattcgaaa gaaaaaatta atggaataat
     7141 gaaaaagtac aacacaaagg cagatgaaat caaccaaaca agaagtgact ggttaaacaa
     7201 tcagacgatc gttctcaatt taagtgaaag cttcagtgat cctgcacgtg tccctaatct
     7261 gacagtacct acagacccta ttccttacat caaaagcatt ataacagaag gaacaggagg
     7321 attgatgtta tccgtcggtt atggtggcgg aacagctaac atggaatggg aaggactgac
     7381 aggcttgagt atcagtaacc tgtctgcttc gttagttaca ccttataccc agctagtgga
     7441 gcgtcagaaa ataagtccaa atattaccga tctattcgat gaaaaaatag ccatccatcc
     7501 ttttacagct gctctttatc gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt
     7561 ctattattta gacagtccgg ataaattaac ttatacggat aaaatagaac aaagcccacg
     7621 catatcggat gattcagcat acaaagaaac cttgaaagct ttacaaagca atacggaaac
     7681 gtcacaattc atccaattat ccactatgca aaaccatatg ccttatgatg attactatag
     7741 tgaattgaac tacactgctg aaggaactgc tgtcattcct aatagaagaa aagaattaca
     7801 aacttatatg caaggccttc actataccga tacagcagta aaaaaattca tccaagaaat
     7861 cgacaaaatc gataaaccga ttacctttgt tttttatggt gaccatttac ccgcaatcta
     7921 tagtgggaat gattcgaaaa aatatggatt agaacagcac cagacagatt atttcattta
     7981 taacaacaag tatagtcgtg aacaagcagc tacgttaaat aaagaagttg ttgctcctaa
     8041 taactttggt gctatggctc ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt
     8101 gttgacagaa gtatctaatc atctacctgc attaacaatt gatcctacag aaagcttgtc
     8161 taatcgtttt aatggaaaac aggtctttgt tacagaggat agtcaaatta tccaagaaaa
     8221 aaatttaact gagaaacaaa aagaaattct agaagattat cgtctgatac aatatgatct
     8281 tgttgcaggt aaccagtaca gtgcaacatg ggcgacacaa aaaataaaag actaacaaat
     8341 aaactgagca aggggttgtg ataaaagaca tgtcacaacc ccttgtttat ctcattaatt
     8401 tgcttgtcca taaacgattt gattaaagcc atttttcaat tcattattcg ctgtctcgtt
     8461 gaagtaaaga taattatcgg gattcccctg attttgttgt gtcaccaata cttttggctg
     8521 attttgctga aacccgaata gatagacgtg ctttttcaga tatggttctg tctctgcatc
     8581 ggaatagacg gctgtgattt gatagtctgc ttgacctgtc ccactttctg accattctgc
     8641 agaaacaggc actccaccaa tagccatctt ccactccccg ttcaaaatag cttgagggac
     8701 tttaagacca tataagtcca cactcatttg attcgtataa cttttgtatt gttgtcccat
     8761 tgtttggccc catttggtca taaatgcctc taattgtgcg gatttgtctg cattccacag
     8821 tgtttgttca acttgttcgc tgctttctga agaggaagat gaagcctcta cagaagaact
     8881 tgaagtagat gtttgagttg tttcagtcgt tttggaagac tctgacgaag tagaggtatc
     8941 tgctgtttct gtacttgctg cagttgactt gctctctgtg ctagttgttg tagtagttgc
     9001 aactgttgct ttcgctgttt cttttgttga ttcttttttc gattcattcc cgttggtaca
     9061 accagctaac aaaaaagcta gaccaattgt tactaaaatc ctttttttca attaaaaaac
     9121 tcctttcgat tactcccact tctttattct atcaaataag caatgtgatt ttattgattt
     9181 ttcgtatttt ctcatcaatc tttcaaatct tctaaaaact gagaagcacc cgctgatgtt
     9241 gtctgtaata catcagcggg tgcttctttt attttaatct tcgtgatttt ccaatttacc
     9301 tcacgcttta tatttgtatt tgaagaaaca tagactttta gtcatcatcg tcttcctcat
     9361 ccaaatcttg tcggacagtt tttccttgga gtctttgtac ttggtagatg atttctttat
     9421 tgaaacttaa aagttcaaca agcaattgtt gcaagccata tttttcttct ttttcagcta
     9481 atttgattgc ccgtgtgata aagacatttt ggaaatcaaa atcttttgca aagttttcca
     9541 acattgttga ggctggtaaa tatttgtttt gaccatgttc gtaaattttt ccataacgca
     9601 tgacttcttc taaagtagac ggaacgattt cattttcaat cactaactca tgagagattt
     9661 tattgaacca tctcgcttct tgatcagcat attctgaaaa aagggtactt aattcttgcg
     9721 aagatgtacc caaaacatac aattgtgctt ggatcaattt catttcttct aatttaaaat
     9781 ttgctaatac atgccccacc atggcgccag cagttggttt atgatggtcg atatctgctt
     9841 gttttgtttc ttgaacaaat ttttcttcca ctatattcat tgatttacgc ctcttttact
     9901 tttgctgatt ggacttcata acctaatttt tccacaactg cagctaattg ttcagggtgg
     9961 acttcatctg gatcaaagtt tgctttgatt ttgctcgcat taaacaaaac tttcgtattg
    10021 cttaccccag gctgttgaga aaccgcatgt tcgatttttt gcatacagct tggacaagct
    10081 agtgtatcta attgcataat aatttttttc atcgtagttc ttccttcctt tttcttgata
    10141 gtcttattct agattacagc ttccataaaa aaattgattc ttatcaagtt ttacgatttt
    10201 tcttctttgc tttcgtgtcc aaaatacaat aaacgcatcg cgttaaagat aacgatcaag
    10261 atactagctt cgtgaacgaa catcccacta gccatgtaaa taaacccggc aaataagccg
    10321 ttgagtaaaa ataagacgac accaatggca atcactacat tttcacgggt atttaaaaca
    10381 gttttcttag ccaagcggta ggcatgaacc aacgattcaa aattagattg catcaagaca
    10441 acgtcactcg tttcgatagc aacatccgtt cctgatccca tagcaatccc gatatcagcc
    10501 gcagccaaag agggagaatc attgattcca tctccaataa aagcaacgcg caatccttgt
    10561 tcttggtatt ttttcacaaa ttgaaccttt tcgtctggta ataattcagc atggacttca
    10621 tcaatgccta acatatcagc tacataatca gcagtcatct gattatcacc tgtcagcatc
    10681 actaactgtt ttgctcctgc ttgtttcaac ttagctaatt gttcagcgac ttctggtcga
    10741 atcacatctg acacacctat gatttgagtg atttttttgt cgattgctac aataatgacc
    10801 gagctgcctg tttgttgtaa ttgatgtaaa tcttgtactt gctttggtgt gagagaaagt
    10861 ttgtgagcgg caatcaattt ggtgttgccc gcacaaactg tatgaccatc aatctcagct
    10921 gtgattcctt gtcccttgat tgtctgatta tctttgactg ctaattgttg aaaatcaatg
    10981 tctttctcct gagcataatg gatcacggcg cgccccagag gatgatcgga taacgtttct
    11041 aatcgagcca ctaaagctaa gctgtcccct tcatcctctg tatagttctt gatagtggat
    11101 acagcagtag ttcctttggt caatgtacca gttttatcaa agacaaaagt atcgattttg
    11161 gaaaaagtat ccattgcttc accgcctttg actaagatgc cccgtttcgc tccattaccg
    11221 attcctgcta cattcgaaac aggcgcacca atgactaaag cgccaggaca accaagaacc
    11281 aaaacagtga ttgctaaacg aaaatcacga ctaatcagac caacaaccaa cgcaataagt
    11341 aaaacggttg gagtataata ctgagaaaat cgatcaatga atttttccgc atgggatttg
    11401 gtatcttgtg cttcttctac taattcgata attttggcaa aggtcgtatc atctccgacc
    11461 ttagttgctt caatcgtcac gtagccatct gctaaaatcg ttccagagaa gacttcatct
    11521 ccactgattt tgacgacttg tcgtgattca ccagtcactg cggcttcatc cgcataccct
    11581 ttcccgtcaa tgactcgtcc gtccacagga atagctccac cagtttttac taagacgata
    11641 tcgccttctt ccacgtcatc gatttccact tcttcagtcg ttccatcttc ttggacaaca
    11701 ctagcagtag atggggccat ctcggtcaaa gccttgattg actcccgcgt tttggtcaat
    11761 gttttttgtt ccagatagct accaaacaaa aacaagaatg taacgatagc tgactccttg
    11821 tattctccga tcataaaggc accaagcaca gcaatagtaa cgagtaaatc aattgagata
    11881 actttgactt tcactgcctg atacgcatgg atcataatag gtatgaccgc aataatagaa
    11941 gctatggcta gagcaatgtt atagaacaag acaaaaccag taaattttcc gataaaacca
    12001 aaaataatca acacagctga taaggcggtg atatgatttc gatgctcttg gatccacttt
    12061 tgtaatttca tctttctccc tcttttcctt tcattcttac gagactaagt atagatactt
    12121 atccatcata aaaaattgac cactatcaag tttagtcaaa aaggcggttt tcttttggta
    12181 gaatacacta caaaaaatag ttagcaagaa gactcactaa aagtgatctt gccaactatt
    12241 gtcgtttcta tcgaaatttt cattgaatga ttttcctgta cagcatacat cggagatcta
    12301 tttctttatc taagattttt atcttgctaa ttttttcctt tagtctttca aatccacgtc
    12361 gttcatagaa ttgatacgta cagccactat ctgtaaaaag gaatatttct tttcctttct
    12421 ctcttttttc aaattctgtt agcaacttgg taccgatgcc ttttgttttc aaatcaggat
    12481 ttgcagctaa aaaaatgatc tctccatctg gcgaatgctt ctttaaatat gctgcaagca
    12541 tttgttcatt tgctgtctcg taagctcctt tactttcttt agcaaatata ttttgtaaaa
    12601 aatcgaatat cttaacatat atttgctgtc caaaagaacg atgtttcttt ttttctcctt
    12661 ttatctccgc cagcaaaaca cctgccaact cgtcctctgc atacaaagca agaatttgag
    12721 ttgctcgatt caactccata tacaaaaaat atcttccgta aagattcagc agaagttcat
    12781 tttccatata tctctcaaag ttcattcctt caacagcaaa atagatagct tttttgtaat
    12841 ctttcttttc taagggttta aaagttagtt tcatcatttc gcctcctcaa cttcaatgat
    12901 tgtgttatac ttacattata atagacacag tgtctactat ctataatcaa tcatctataa
    12961 gtttgtcgat taagcgacag ctttgctttg atatgtcgga taaggagata ggtaaatgaa
    13021 aaagacagat agacgcgtca aaaaaacaga aaaagctttg gctgaaacac tatccacatt
    13081 attagtaaat aaaaagattc aagcaatcac tatacgcgaa ctaacagaaa cggcagatgt
    13141 acatcgttcg actttttata cacattataa ggatatctat gatctatatg atcaattaga
    13201 aagcaacttt tttcgggatc taaatgaaat tctctcttat gatccagccc atagctatga
    13261 ggaactatac actcgcttga ttgactatgt ttacgcaaat acttttttat atcgcttatt
    13321 tatcctcaac catggtgata tttcgtttca gaagaaagtt aaacaagtta ttgaagaaaa
    13381 ctatctgaac atatggctat atgaagacaa gaaaacgatt atcacagaag aaatgagatt
    13441 ctttactacg taccacgtcc aaggttgtct atccgttctc aatcggtggg tagaaagcaa
    13501 ttacaagtac tccaaaaaag aattattaga actcttacag aaactgaatg atcacataga
    13561 gaaaatcatg ccttgaataa aaacatacta agcttagtta agaagaaccg taaccttctc
    13621 gtataacaac tgaatcttat ttagccaaat tgtttactga agattttttg agtaaaattt
    13681 ctataattcc tacttattga aaagtagttt gtagtgctgc ttctccctct ctaagtgata
    13741 tatcttgttg taccaatcaa attcacctca tcattaataa attactttta ggctaattta
    13801 ttacaactta taggttgaag ctgtaaccaa tttatagtca tattatattt ttatcatcca
    13861 gtttattgaa ttcttctcgt ttacctcaat tcattttcaa ggggaatcag ctcatcatct
    13921 aacattttac gccatgctcg cttcatttct tgtgggtatt taaatggttt cgcttctgga
    13981 tgtttggctt tatatccttc gtaatcctta ctgttgataa ttgctttcat attaggaatt
    14041 ggatcttcac ctggcatata ttgcagatta gaagcatgta attttttctc agaaacaaac
    14101 cactcattgg aaaagacatg aatcaaatca tcacttactt gtgtaaaata gcgacgttta
    14161 atgacaaaga tatcttcatt gctatctagt gtaccttcat ttctttgtgc ttaaattcaa
    14221 agataatttt ctcaataaag atttttgatt ccagtaagat tcctttactc cttttacaac
    14281 atagcgttgt cctttgtttt gaaacatctt cttcccctct ttgccattaa aaatagccca
    14341 attctctata aagaagatta agcttcatta ttataatata aagtttaaat ttggtttatt
    14401 acttactttt tcttattctc tagtaagcta atattgaa
//
LOCUS       DAZAFF010000070        14228 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000070 DAZAFF010000000
VERSION     DAZAFF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.070"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..272
                     /locus_tag="ACG9BB_002057"
     CDS             39..272
                     /locus_tag="ACG9BB_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN2837987.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(371..802)
                     /locus_tag="ACG9BB_002058"
     CDS             complement(371..802)
                     /locus_tag="ACG9BB_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFN2837988.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            881..1294
                     /locus_tag="ACG9BB_002059"
     CDS             881..1294
                     /locus_tag="ACG9BB_002059"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFN2837989.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(1332..2480)
                     /locus_tag="ACG9BB_002060"
     CDS             complement(1332..2480)
                     /locus_tag="ACG9BB_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFN2837990.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(2602..3198)
                     /locus_tag="ACG9BB_002061"
     CDS             complement(2602..3198)
                     /locus_tag="ACG9BB_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFN2837991.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(3398..6040)
                     /gene="alaS"
                     /locus_tag="ACG9BB_002062"
     CDS             complement(3398..6040)
                     /gene="alaS"
                     /locus_tag="ACG9BB_002062"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFN2837992.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            6428..6859
                     /locus_tag="ACG9BB_002063"
     CDS             6428..6859
                     /locus_tag="ACG9BB_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2837993.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(6904..8253)
                     /locus_tag="ACG9BB_002064"
     CDS             complement(6904..8253)
                     /locus_tag="ACG9BB_002064"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFN2837994.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(8323..9486)
                     /locus_tag="ACG9BB_002065"
     CDS             complement(8323..9486)
                     /locus_tag="ACG9BB_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN2837995.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            9615..9938
                     /locus_tag="ACG9BB_002066"
     CDS             9615..9938
                     /locus_tag="ACG9BB_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2837996.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(10121..11470)
                     /locus_tag="ACG9BB_002067"
     CDS             complement(10121..11470)
                     /locus_tag="ACG9BB_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFN2837997.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(11539..12492)
                     /locus_tag="ACG9BB_002068"
     CDS             complement(11539..12492)
                     /locus_tag="ACG9BB_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFN2837998.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(12594..13784)
                     /locus_tag="ACG9BB_002069"
     CDS             complement(12594..13784)
                     /locus_tag="ACG9BB_002069"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFN2837999.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(13984..>14228)
                     /locus_tag="ACG9BB_002070"
     CDS             complement(13984..>14228)
                     /locus_tag="ACG9BB_002070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838000.1"
                     /translation="RKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVF
                     LLVYAINKLNIQNTIDFNERASINGCVIFICPGRVK"
BASE COUNT         4177 a         2951 c         2460 g         4640 t
ORIGIN      
        1 gaaggctatg atcagacttt agaaaggtcg tgagtgcttt gaccacagga attgtaaaat
       61 ggtttgacaa caaaaaagga tacggcttta tcagttatga tgacacagaa gaaatttttg
      121 tacattttac tgcgattgaa gaagagggat tcaaaacttt ggaagaaaat caagtggtcg
      181 aatttgagat catcgaaggg aatcgaggaa cacaagctgc ccatgtcaag aaaggcaacg
      241 ttcagactac cgatactagt atgcatgaat agataaacaa atagagtgag acgctaaacg
      301 ggatgaaccg tacgtctcac tcttttcttt tcctgtaaaa attcgttttt ttaggttctg
      361 atttttaaaa ttagcttttt ctttttttcg tataaggata aggaattgat tctgatttgg
      421 gtccgttttt ttcaaaatga tcaaaaagga ctacttgatc tttttttcgc atcatgaaaa
      481 cagatggggc tttctcatct tgcaacatca tttgcacccc agtattagtt ggcgtcaaag
      541 atcgaatctg tccgacagat atttctcggt ctttccaaaa agcagcataa cgtataatca
      601 cagagtttga ttttaaaata aaagaacgcc gcaaacctag aaatagaaaa acaagaaaaa
      661 tacctaaaac aaaattgctt ttccaataag gacctgtgtt ttctaaagaa agaatcagac
      721 taaaaaacag aatgatcaag gtcaatgacc aatagatgat cgtatgtgcc agttcaggct
      781 gccatcgaat attttttttc attctctaac ctctcaccga ttcagtaatg atataatctt
      841 accataacat tagtttgaac aaaaaggaga atctcttttt atgttaaaag tatacattga
      901 tgcctctaca aaaggcaacc ctggtcctag tggaggtgga atcttgattg tctatcaagg
      961 taaacaagaa cagctatcta tcccgctttc tatagggaca aaccatcaag cagagttcga
     1021 agtcttttta aaaactttag aatttctaaa gaaaaacgac ttacagaatg aaacgatttt
     1081 ctgtttttca gacagcaaaa cactagtttc tacggtcgac aaaaacgcaa cgaagaacga
     1141 gctcttcctt ccctaccttc aaagaatcca agaacttcta tccgaatttt ctttattgat
     1201 cctgcaatgg ttgcccgaaa acaaaaataa aggagcagat aatcttgcac gtcaggctct
     1261 ccataaacaa ttaaaagaaa aaaagactct ttaaattaaa aactttaagg agtcttttta
     1321 tagtttctca tttaatataa aagataattg tgatcaaacg taatttccaa ttggtaatct
     1381 ccgatttttt cttctatttg actacgtaag attctttcta gttctgtatc aacaaactgg
     1441 aatttctcag ggaccactac atcaaattgc agcgtttttc cttgatgaat tactctaaaa
     1501 tcatgaatat taagccctgt acctgtttca agaagaattt gctttaactg ccgcaagata
     1561 ttatagtatt tttcgttatc aacaggcatt ggatccaagt gacagacaag ttccacatcc
     1621 aaagactttt taaagtcttt ttctatcaga tcaataatct tatgagccac attcaaattc
     1681 attgattcat ctacttcgat gtggactgat gcaaagcgtt tattgggtcc atagttatgc
     1741 accaataaat catgatagcc aagtatatca tcgcaagcat ttaaatgctc ttccatttct
     1801 tcaatctctg ctggcgttgg gcgacttcct aatagttcgt atacaaaatt tcgaagcatc
     1861 gtcaatccac tataaaggat atataatgct aaagcaaaac ctatatatcc atcaatacgc
     1921 catccagtta cccattcgat tgctgctgaa agcaatacag ccagtgtagt aaagacatca
     1981 ttataactat ctgttgctgt agctctcaat gtttcagaat caatggattg cgcaataacc
     2041 tgatacattt tgccttgtgc aaatttcaaa aagatggaaa tgattaggac aaaaaagacg
     2101 atgggtgtta aagagacatt ttctggatgg atgatttttt cagcagaaga ctctaaaaat
     2161 tgaaatccta cataagtaat gatgatcgaa acaaataacc cactgatata ttcgaagcgt
     2221 tcgtgtccat aaggatgttc ctgatctgct ggctttgcag caattttaaa tccgatcaaa
     2281 gtcaatatgg atgaagcagt atctgataga ctgttcactg catcagccat aatggagaca
     2341 cttcctgaaa aaagcccgat cattagctta gagacaaaca agatcagatt cgtaaccaat
     2401 cctaagatac ccgctagtac tccttttttt gtccgctgtt gcggtatttg tttctttttt
     2461 tcttcttgtt tcttagccaa gttttttacc ccttctagtt tgcttgttgc tcaggtaaaa
     2521 agatggagag gacgattgtt ttcctctcct ctcttccttt ttctatctat cgtgcgtcct
     2581 attactatta aaagatggtc attattctgg tttcaacggt gttgctgcaa ataacgcttt
     2641 tgctagtaat ggtgttatga ttcccgcacc tattgcttct ggtatcccat tgattgctgc
     2701 aacccctgct aaggttttaa ataaaagagc agagtctaca ccataggcat ctgccgttgc
     2761 acctgtataa aataatccca tgaatcctaa cactaagact gtattgacca atgaacccag
     2821 tacccctgca aaggctgagc tgatcgttat tgatttattt ttttgataaa tccagtggaa
     2881 aaccaagcct gctactaagc caacaagtac tctgggaact accgagataa tcggattcgt
     2941 gaacactaat gtatcaaatg gcgttgttgg catggcatag gcacgaacaa ttgtagcgat
     3001 tccccaaaac aagccaatca ccatgccatc ttttgggcct aatactactg cagcaataat
     3061 tactgtgata tggataattg ttaaactaat gaatcctaat ggaataaacc ccagccaagg
     3121 aaccatcgct tgtacgatga tgattgctaa caagatcgca cgtaagacta gtcgaaaggt
     3181 cttatttcta ctattcattt tttcacccct ttttatttat actaatcgta aaaatttctg
     3241 tcactttcgt caactaaaga ccatccgaat ccgaccattt gattagatgc gccatccaat
     3301 tgaaaaaagt atgagggcaa gacttgtttg tcttcgcccc cataatcatg tttcgttcaa
     3361 tgtttgaact tttgtttttc tgtcaaacgt aaacgcccta ttgttgcgca acccattctt
     3421 tcgcagcttt aagtgcatct ggaataccag caggattttt cccgccagct tgtgccatgt
     3481 ctggacgtcc accaccgcca ccgcctactt tcggtgcgat tgctttaatc aagtcgccag
     3541 cttttagtcc agcttcattt gattctttcg tcattgccgc tagcagactg acttttcctt
     3601 cttgttcgtt tgctaaaaca agaatatctg aaatattttt ttgtttccat tggtcagcta
     3661 attgacgcag ctgattcata tctttcactt ttacttgtgc tgcaatgatt gtttttccat
     3721 tgatcgtttc tggatctttg aatacatcac cagcttgttg attcgctaac ttgctagcta
     3781 attgttcatt ttccttttgt agctctctta cttgctgttg caattgttcc gcttttgaaa
     3841 caacttcttt caattgaggt gatttaacaa ttgttgctac ttgttttagt tgtttttctt
     3901 cttcttcaag tagttcgtac gcttctttac ttgttacagc ctcgattcgt cgcacgcctg
     3961 caccaattcc tgattcagaa acgattttga agattccgat gttttctgta tttgctacat
     4021 gtgtgcctcc gcacagttcg attgaccagc cgccaatatt tactactcga acgtcatgtc
     4081 cgtatttttc accaaacaaa gccattgcac ccatgttttt ggctgtatcg atatctgttt
     4141 caattgtttc aactgggata gcttcccaga ttttttcgtt gacgattctt tccatttcag
     4201 ctaattcatc tgaagtgatt tgaccaaagt gggtaaagtc gaatcgtaaa tgtccaggag
     4261 cgactaaaga accagcttga tttgcatgct cgccaagtac ttctttcaat gccttgtgca
     4321 gtagatgggt tgctgtatgg tttttgatga tccgattgcg catcgattga tcgatcaata
     4381 gatgataggt ttgtccttca ctcattggtg cttttacttc tacggtgtgc ataaattgac
     4441 cattaggcgc tttttgaaca tctgtaacaa ctgctacgat ttcgcctact tcattcatga
     4501 tccatccttg atccgctact tgcccaccca tttctgcata gaaaggtgtt tgatcaaaga
     4561 tcatttgagc ttcaccttct gcgacttcat tcaaaagaga ctcaccttta acgatgacca
     4621 ataattgact atctgattct tgttctgtgt atccaatgaa tttactttct actttgatat
     4681 ctgtcaataa agcagattgt acccccattg atttttcagt actgcgggca gagcgagcac
     4741 gttcgcgttg tgcatccatt tctttttcaa aaccagcatg atcaactttc agtccttcat
     4801 cttctgccac ctcttccgtt aattctaccg ggaagccata tgtgtcgtat agtttgaaga
     4861 tgtcttttcc tgctagagtc gtcccgtttt cgttttttac ttcttggatc acttgattta
     4921 agatttccaa accttcattg attgtttcat tgaaacgttc ttcttctgta cggataactt
     4981 tttcaataaa ttctttttgt tgaagtactt caggataata acttaccatg atttccccaa
     5041 caacagggac taaacgatat aagaatgctt gttcaatacc taattttttc ccatgcatga
     5101 cagcacgacg aagcaaacgg cgtaagacat accctctccc ttcattcgat ggcaatgcac
     5161 cgtcgccaat agcaaatgaa agggcgcgaa tatgatctgc gattactttg taagaaatat
     5221 ctgttactgg gttttcacca tattttactt gtttgcttag cttttctacg gcgtggataa
     5281 taggcataaa caagtctgtc tcaaagttcg ttgacgcatc ttgaacgatt gagaccatgc
     5341 gttccaatcc catccccgta tcaatgtttt tatgaggaag tggttcaaat gtgttatcag
     5401 gcttatggtt gaattctgaa aatactaagt tccagatttc taaataacgt tcattttctc
     5461 ctcctggata gttttcagga tcgtcttctg ctaaatcgtt ataagcttct ccacggtcat
     5521 agaaaatttc cgtatccgga ccacatgggc ctgcaccgat atcccagaag ttgtcttcta
     5581 catcaataat gtgatctagt ggcaggccaa cttcttcatg ccagatgcgt tttgcttctg
     5641 tatcttttgg ataaactgtt acataaagtt tttctggatc gaatgccatc cattttggat
     5701 cagtcaaaaa ttcccatgcc cagtggatgg cttcattttt gaaatagtca ccaatcgaaa
     5761 aattacccag catctcaaac atcgtatggt gccgtgctgt tttaccgaca ttttcgatat
     5821 catttgttcg aatagatttt tgggcatttg tgattcttgg attttctggc acaactgaac
     5881 catcaaaata ttttttcaat gttgcaacac cagagttgat ccacaataag gttggatcgt
     5941 tcacggggat caaagacgca ctaggttcaa tcgtatgtcc tttagattgg aagaaatcaa
     6001 gaaacatttg tcgaacttgt gcactactta attgtttcat gtcattacct gctttctata
     6061 aattaaatac aaaaaaacac catcacagcc aaggacgaaa actcgcggta ccaccttgat
     6121 tgcaatgatg tgtaatcatt acctcttaaa ctcgataacg ctgagctgcg tcggtatttc
     6181 taccaaaata gaaaaaggga gcaatttgat tagtgactat tcttttttca gccaagaaag
     6241 aattttctgt gagtcttaaa aaacaaatca tatccttttc gtacttaatt agtatacgca
     6301 ttctattgat cactgtcaat atctcaattt ttattttcat ttccttttgg tcatactcca
     6361 cgatttttgg tacgataaga ataaaggaat caatacatct ctgctattca tgacaaggag
     6421 gaatatgatg aaaaaaatag ttggagcgga tgaatggata cgcgcagcta gctgcttttt
     6481 gagatatcaa gtttttgtat tagaacaagg gattcctgca gaactagaat tcgacggaaa
     6541 agatatcccg caacaaacct attatctatt tcttgatcaa aaaagaccag tagctacttt
     6601 gcgttatgaa aagaaaacac ctacttgctt caagccggaa cgattttgcg tagcaaaaga
     6661 ataccgagga aaagggttag gcggtcagtt gcttgaagct gctgaaaaac gggcggtttc
     6721 agaaggttgt cagactagtt atgtagtagc tgaaatgacg gctgtagatt tttatgaaaa
     6781 acaagggtat catacttgca cagaaccttt tattgaagat gaagtacttt gtatcggaat
     6841 gaaaaaaagt ctagcttaaa aacaagagat tgtgacacgc cttttgtcac aacctcttgt
     6901 tttttattta tgtgaatttt tcttattttt acgagcagta cggctttgct gtctgcgttc
     6961 gattttgcgt tcttttttat ttttttctgc aattgcccaa tcgatttttt tcttataacc
     7021 aggcttgatc tttttctttt tcttcttcac aagaccaatc agcgtgggat ctaatacatc
     7081 ttttgatttt tctcgtttgg cacgtctatt acgatcgtat gttttgacga tttccccgtt
     7141 tttgatttct tttggttcaa atgagacgcc caatttttct atcgcatcaa tggcttgctc
     7201 atcatttggc gaatataacg taatggccgt tccttttaag ccattacgtc cagtacgtcc
     7261 gacacgatgg ataaagaagt ctagatcttc tggaatttct gcattaataa catgagagac
     7321 accttcgata tcgatccctc tagcagctaa atcagtcgct acaacatatt gatactccag
     7381 attctgtact tgtcgcatca ctcgttttct ctctcttggg gtgatgtctc catgaatctt
     7441 tgctactttc aagccttggt ttttcaagta atcggcaatt tcatccacat gctgttttgt
     7501 attggcaaat acgattgcta aataaggatg accaattgtc agcagttgat aaatcatttg
     7561 attctcatct ttaccttttg ttgaaatcag ccaattttca atgtcttcag aaataactgc
     7621 ttttggtttg atttcttcaa tgattggatt ttccatgtat tttcgtaaga atggacgaag
     7681 tttttctgga atagtggcag aaaaaactaa aaactgcagc ttttctggta atcgacttgc
     7741 aatttggtct acttcattta aaaaacccat atccaatgtc atatccgctt catcgacaac
     7801 aaatgcaaat gcggtgtgaa tcttcagcgc ttgttcattc atcatgtcta atattctacc
     7861 aggcgtgcct actacaacct gtggctgttg gtgatgcaga cgatttaatt gacgctgttt
     7921 gtctgtaccg ccgacaaaat tcgagacacg gatctccggt gtagaaaatt tagcgatttg
     7981 tgtagctgct tcatagatct gtgcagctag ttccctactt ggagctgtaa taacaacttg
     8041 tacttcctct ttttctgcat tgattttttc aaacaaaggc aacaaaaacg tatgtgtttt
     8101 tcctgtacca gtttgagatt gcccaatgat attttttcct tttgcaatta aaggaatcaa
     8161 acgttcctgg acctctgtgg gctgtttgaa gccctttgcg gttaacgctt cattgataaa
     8221 cggtttgaag cggaatatat caaaattatt cattcgtgca cctcttctct ttaaattatc
     8281 acgactttgg cattataaca cagacaaagc gtttttgctc agctaatttc gagaatcatc
     8341 atcctttgtt tggccaatcg tgctaacagt atatgtgtga ctagcttttt cataaaacag
     8401 aatccgtttt tttctcaaaa atgatagcaa gatatttaaa ataaaaatac cttgtagtaa
     8461 caagacaccg agagaaatga atactgcact catctgcaac accgtattcg ttgtcgaatt
     8521 catgatttgc tgaagcaact gccccaacca gcttaaacca taaaaaagaa agtacagata
     8581 cccgtagcgg atcatcaatg cttttaaaga aattcgttcg cggtcttctt ccaccaaacg
     8641 gatacgcaca atttttttgc caagtgtcca tccattggtt ataaacggta caatcataaa
     8701 gtatacccct acttctaata aaagccacca gatatgagaa gtaactgccc ctgaatcgaa
     8761 cgcaatttta aagaaaatcg ccgctaccaa attaataaga gataaaaatg tccagtcgat
     8821 caaataagcg accaagcgtc gagtcaatga gacagtcttt cctttttcat aagaaagttc
     8881 atccattcgt cttcttgtag gaagcataaa tgtaaataac ggcgtaacga caaaaccaat
     8941 gattccaccc aatgtatttg tcactaaatc attcacatca aataagcggt atggtcttgg
     9001 gtagataaaa taaagtccac ttagctgcgt caattcaaaa aatagagaaa ggcagaaact
     9061 agcaatcatc gttttcataa aagaaagccg gaaataatat cttaaataaa tgccaaacgg
     9121 tatgagtaat aaaacattaa aagctggttc caaaaataca ctctgcttca ttgccggcag
     9181 gtaggtacta ggttcagaca agaccaatac cgtgttgtct ctgaaatgtt gccatgaagc
     9241 aaataaatgc agctccatct ttggccctgt catttgggca accgtctcct ttgccggaag
     9301 tggtaagatc accaaaaaat acgcacacaa taaataaaaa acgaaagaat acaaaataac
     9361 tgcccttgtt actagaaatg ttccatactt tcgatattca taaataaaaa aaacagatga
     9421 gatcgctaag gctaaaaatg gaaaaacaat cagcgcaaaa cgaatcggtt cgatataact
     9481 tgccatatga ctcctccttg attctttgta tctctcttat cttagcagtc ctttgctgtt
     9541 tcaagcaagt aacgaggcta ttatttctca tttctttcaa agtaaagtaa gataaacaca
     9601 aagggggtgt cgagatgctg aatatgtact ttgtttttgg cgtacctatt tttttactgt
     9661 tcctttatgc tacgatcgct tatgtaagaa aaagaactac aattcattat ttaggattta
     9721 ttctcctgat tatttcgggt ttcatgctag tcttcaatct tcagacttgg cagcaagcac
     9781 ttcttgaaat ggataaaatg actccacatg ctttgtctaa agtgcttggc taccctgtct
     9841 atctgatttg gcttcccatt ttcatttctg gttgcttggt tttattaaat atttatcgcg
     9901 gcgtacgccg aatagttcag ttacgcaaat caaagtaaaa gacagaaatc ggttgagccc
     9961 gatttctgtc ttttactttg ctatttcatc gtagacaaaa cgacatttac gtgatcaaaa
    10021 ttaacgagta cttttgacat ttcttccacg tactcctcat ttaaataaaa tattttgttt
    10081 ggcagagaag tgcagttcta ataaaattcg aatgttttag ctatctacgt tttaaataga
    10141 gataaatcgg gataccagca agtgtcaata ctatccccat cgaagctaaa aaggtctgag
    10201 tgataagcgt gcttgatacg ataaaagtcc ctccaatgat tgctactagt ggaataaacg
    10261 gatacattgg aactttgtat ggtcgaaaca aatcaggttc ttttttacga agaagaatga
    10321 caccaacaaa gaccatcgta taaaaaatcc aaatgacaaa gatcagcata tccgttagtg
    10381 tatcaaaacc accgatcatc atcatgccaa tagcgatgac tagctctaag atacctgcgg
    10441 ctacaggaac tttagtttta tcatgaagtt tagcaaagag cttgctaaaa ggcaggttgt
    10501 tttcttttgc cataacataa gggagacgca ttccagtaag ggtatagcca ttgatggttc
    10561 cataaacaga aatcaagata ccaactgtga ccagtcttcc accgaagccg ccaaaaatca
    10621 tttttgcaac gtcacttgcc gcattgctgt ttcctgcgac tccgtcgata gatgcggttc
    10681 ttaagaacac cgcatttact aataaataaa cgatcatgat tccgatgatt cctaaagaaa
    10741 ttgcttttgg caaatctttc gccggctttt tcaattctcc cgaaatattt cctacgtgta
    10801 tccaaccgtc gtatgcgaac atcgttgcta gcaagcccgc tcccaaggct gagaaaaatg
    10861 aaagattttc tcctgcttgg atcggaaaca gttggaagtc tactccttct tgtcggaaca
    10921 aaccgaaaat gacgatcaca aacaaaggaa taagcttgca gacaagcgta atcgactgaa
    10981 aagcccctcc agctttagaa cctaaaaaat tgatcaacag gatcgataca gcagctgtaa
    11041 cagctacggg aacaatcatt gactgggaaa gaccaaatag attgacgaac tgtgtaccga
    11101 aaatgatcga caaggctgct acatttgctg gaaaataaat cacgacttgc gcccagccca
    11161 ataaaaaagc tgctgtattt ccgtaaatcc gttcgatata tttaatcatc cctcctgttt
    11221 ctggaatagc agctgccagt tcagctcctg ttagtcctgc acagacagaa atcataccac
    11281 cgagaaacca agaaaacata tgcaaactgg cggatcctgt aacttctgct acgcttgcgg
    11341 ctttaaaaaa tacccctgca ccgatcactg tgcccataac agtcgataaa gcagtaaaaa
    11401 atcccatcgt acgttttaat tcattttcta tttttacttc ttttactggc gtggcttgat
    11461 attcttccat cccttgtccc ctttcatacc atagccaatc ccaaaagtat aaaaaacttt
    11521 ttggatgtgg ttcttgtttt ataccgtctc taccaatttt tctttcagtt tttccaacat
    11581 atcctttgtc atcacatcta agtcgtattt cggcgaaaag ccccattcct cttgcgcaca
    11641 agaaacatct agtgaatttg gccaagattc tgctattgac tgacgtacag gatccacatc
    11701 ataatccatt tcaaaatctg gtatgatttt ttgaatagat gctttgatct cttccgggtc
    11761 aaaagacatc gccgatatat tgaaggcatt tcgatgaacc aatcgatcag gatctgcatt
    11821 catgagtttt ataatggcat cgatcgcatc tggcatatac atcatatcca ttgatgtacc
    11881 ttgatcaatg aaactagtgt actttccttc tttgattgct gaataataaa tatcgactgc
    11941 gtagtctgtc gtaccgcctc ctggcaaggt tgcataagaa atcaatccag ggaaacggac
    12001 accacgagta tctacgccat atttcacata ataataatca catagtagtt ctccagctac
    12061 cttcgttacc ccatacatgg ttgtaggacg ctgaagagta tcttgtgggg tatgatcttt
    12121 tggagtattt ggaccaaacg cgccgattga acttggcgta aaaaatttca gctggaattc
    12181 tctagcgact tccaatgcat tgaccagccc agtcacgttc aaattccagg ctaattgtgg
    12241 ttttgcttct gcaacagctg acaaaagagc agccaaatga atcaatgtgt ccacttgata
    12301 ttgttctgct atgctgcgca ttttttcgta atccattaca tctaggatct caaagatatc
    12361 gtttttctca atgacttcag gcatgcgtat atctgtcgca atgacatttt cactgcctag
    12421 ttcttgacgt agccgtgtga tcaactctga accaatttgt cctaaacatc ctgtcaccaa
    12481 tacttttttc atgatgatcc tccctgatct tttttactac tatcttcaga tgattaacaa
    12541 tatatgcgtg aacatgttct ctaattttaa atagacgaaa aaactaaatt tagttaaata
    12601 atttccattt cttttcctat tttttgataa actgctactg cttcatcaag catttccctc
    12661 gtatgttccg cagtcggcat atttctgatc ctgcctgtcc caagaggcac agttggataa
    12721 acgattggtt tagcataaat cccatgttcg atcagcttct tagagaaagt ctgagtcact
    12781 ttttcgtctc ccaaaatgac tggagtaata ggtgtttcag acataccgat gttatagcct
    12841 acttttttca attcttcttt aaaataatta gcattttccc aaactttttc gacaagttcc
    12901 ggatgttctt gcatcaaagt aatcgaagcc aatgcagctg cagctgcacc tggagttagc
    12961 gaagtagaaa ataggaatgg ccgacttcta gctttcaacc agtcgattag tgttttcgat
    13021 ccagctacat aacccccaac aacacctata gctttcgata acgtgcccat ttgcatatcg
    13081 attttgtcgc ttaatccaaa atgcttgacg gttcctgcac cttttccagt tacgcctgag
    13141 ccgtgcgcat catcgacgta ggtgatcagt ccatattttt caacaatcgg aataatttcc
    13201 ggcaatcgtg caatgtctcc atccatggag aaaacaccat ctgtgatata catgattttt
    13261 ttatatttct tactttctat cgcttccttt gcttttttct caaggtcttt catatcttga
    13321 tgtttaattc ggatgatttt tgctccggat aagcggcaac cgtcaataat agatgcatgg
    13381 tttaattcat ccgataagat agcatcttct tttgtcatta ctgcagagat agcgcccata
    13441 ttgcagttga atccagattg aaaggcgatt gctgcttctg tccctttgaa ttctgcgatt
    13501 ttttcttcta attgctgatg gattttcagc gaaccattga tcgttcggac tgctcctgct
    13561 cctacaccat aagtttcggt tgcttcaata catgcttttt tcaattcttc gcgattggca
    13621 aatccaagat aattgttgga tgctagattg atcatctttt ttccgtctac gatgatacaa
    13681 gcaccatttt cactttctag tatgtcgatc gtattgtata accccttttc tttcagttga
    13741 tccaattctt tctctaaata cattgttagt tctttggaag acattgtctc cacctccata
    13801 ttcatctgtc ttgcagaaca taccaaacat atatcgtgtc attcatctac aaagcaagta
    13861 tgctatattt ttgataacgc ttacactgat tatgagaatt atttaaccac attttttatt
    13921 tacagtatct ttttactttc aaatgactta tatagtatga acaattacct tttagataat
    13981 gatctatttt actctacctg ggcagataaa tataacacaa ccattgatag aagccctttc
    14041 gttaaaatca attgtattct gaatatttaa tttatttatt gcatagacca ataaaaatac
    14101 ataaaatttc catcttcgtt tatcagcaat ttttaatttt actctctgaa tatttcttaa
    14161 aagttttact tgttgaattc gaaaaaagga ctgtgacaaa agtcttgtca cagtcctatt
    14221 cttccgaa
//
LOCUS       DAZAFF010000071        14139 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000071 DAZAFF010000000
VERSION     DAZAFF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.071"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="ACG9BB_002071"
     CDS             complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="ACG9BB_002071"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFN2838001.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="ACG9BB_002072"
     CDS             complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="ACG9BB_002072"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFN2838002.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8128..9096
                     /locus_tag="ACG9BB_002073"
     CDS             8128..9096
                     /locus_tag="ACG9BB_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2838003.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9114..10019
                     /locus_tag="ACG9BB_002074"
     CDS             9114..10019
                     /locus_tag="ACG9BB_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2838004.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10016..10822
                     /locus_tag="ACG9BB_002075"
     CDS             10016..10822
                     /locus_tag="ACG9BB_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2838005.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10979..11947
                     /locus_tag="ACG9BB_002076"
     CDS             10979..11947
                     /locus_tag="ACG9BB_002076"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFN2838006.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12264..12788)
                     /locus_tag="ACG9BB_002077"
     CDS             complement(12264..12788)
                     /locus_tag="ACG9BB_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFN2838007.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13075..>14139
                     /gene="scm"
                     /locus_tag="ACG9BB_002078"
     CDS             13075..>14139
                     /gene="scm"
                     /locus_tag="ACG9BB_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFN2838008.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         4003 a         3047 c         2793 g         4296 t
ORIGIN      
        1 caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt
       61 tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca
      121 ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta
      181 ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc
      241 ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat
      301 aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt
      361 gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct
      421 aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc
      481 atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa
      541 acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac
      601 aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct
      661 tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct
      721 ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct
      781 gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca
      841 cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc
      901 gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg
      961 acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt
     1021 tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt
     1081 tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca
     1141 ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg
     1201 atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct
     1261 tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg
     1321 gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt
     1381 tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca
     1441 ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca
     1501 cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt
     1561 ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg
     1621 tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca
     1681 ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata
     1741 ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt
     1801 tctgttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg
     1861 aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca
     1921 aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg
     1981 aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt
     2041 tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt
     2101 gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag
     2161 accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc
     2221 atatcttggg aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca
     2281 gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc
     2341 atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt
     2401 gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt
     2461 gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg
     2521 tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga
     2581 acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca
     2641 cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca
     2701 cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga
     2761 gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc
     2821 aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca
     2881 ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca
     2941 ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt
     3001 ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat
     3061 gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc
     3121 aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta
     3181 atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga
     3241 tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact
     3301 acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga
     3361 cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca
     3421 atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca
     3481 caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt
     3541 gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag
     3601 ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa
     3661 gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat
     3721 ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg
     3781 gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct
     3841 tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt
     3901 gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc
     3961 tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc
     4021 aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct
     4081 tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa
     4141 gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca
     4201 ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa
     4261 cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca
     4321 gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg
     4381 ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat
     4441 actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat
     4501 acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt
     4561 gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta
     4621 tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa
     4681 acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt
     4741 ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact
     4801 tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt
     4861 accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata
     4921 cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg
     4981 cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata
     5041 tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca
     5101 tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg
     5161 ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa
     5221 tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca
     5281 tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat
     5341 tcaacgacac cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat
     5401 tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc
     5461 atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
     5521 gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat
     5581 acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct
     5641 tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag
     5701 tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg
     5761 ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt
     5821 gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga
     5881 acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt
     5941 ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa
     6001 ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc
     6061 aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc
     6121 ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag
     6181 tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa
     6241 gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc
     6301 acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt
     6361 ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca
     6421 atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg
     6481 atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt
     6541 ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt
     6601 ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct
     6661 ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt
     6721 ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag
     6781 atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt
     6841 gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct
     6901 ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca
     6961 tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg
     7021 atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct
     7081 ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag
     7141 tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct
     7201 acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt
     7261 tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt
     7321 ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat
     7381 tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc
     7441 gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc
     7501 ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc
     7561 ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc
     7621 ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa agtcaacact
     7681 tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc
     7741 aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc
     7801 attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa
     7861 taacttttac tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta
     7921 aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag
     7981 acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt
     8041 tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg
     8101 tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct
     8161 gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat
     8221 ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat
     8281 aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc
     8341 gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc
     8401 aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat
     8461 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
     8521 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
     8581 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
     8641 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
     8701 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
     8761 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
     8821 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
     8881 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
     8941 ggtaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
     9001 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
     9061 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
     9121 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
     9181 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
     9241 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
     9301 cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca
     9361 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
     9421 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
     9481 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
     9541 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
     9601 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
     9661 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg
     9721 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
     9781 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
     9841 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
     9901 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
     9961 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
    10021 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
    10081 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
    10141 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
    10201 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
    10261 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
    10321 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
    10381 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
    10441 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
    10501 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
    10561 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
    10621 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
    10681 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
    10741 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
    10801 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
    10861 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
    10921 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
    10981 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
    11041 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
    11101 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
    11161 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
    11221 acaaacgatg ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg
    11281 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
    11341 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
    11401 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
    11461 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
    11521 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
    11581 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
    11641 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
    11701 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
    11761 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
    11821 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
    11881 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
    11941 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
    12001 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
    12061 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
    12121 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
    12181 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
    12241 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
    12301 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
    12361 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
    12421 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
    12481 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
    12541 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
    12601 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
    12661 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
    12721 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
    12781 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
    12841 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
    12901 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
    12961 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
    13021 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
    13081 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
    13141 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
    13201 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
    13261 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
    13321 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
    13381 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
    13441 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
    13501 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
    13561 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
    13621 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
    13681 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
    13741 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
    13801 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
    13861 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
    13921 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
    13981 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
    14041 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
    14101 acagatacat ctaccacaag tgatagctca gattcatca
//
LOCUS       DAZAFF010000072        13948 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000072 DAZAFF010000000
VERSION     DAZAFF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.072"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            213..1076
                     /locus_tag="ACG9BB_002079"
     CDS             213..1076
                     /locus_tag="ACG9BB_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFN2838009.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1205..2827)
                     /locus_tag="ACG9BB_002080"
     CDS             complement(1205..2827)
                     /locus_tag="ACG9BB_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFN2838010.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3076..3597
                     /locus_tag="ACG9BB_002081"
     CDS             3076..3597
                     /locus_tag="ACG9BB_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFN2838011.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3783..5513)
                     /gene="spxB"
                     /locus_tag="ACG9BB_002082"
     CDS             complement(3783..5513)
                     /gene="spxB"
                     /locus_tag="ACG9BB_002082"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFN2838012.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5678..6496
                     /locus_tag="ACG9BB_002083"
     CDS             5678..6496
                     /locus_tag="ACG9BB_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFN2838013.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6599..6796)
                     /locus_tag="ACG9BB_002084"
     CDS             complement(6599..6796)
                     /locus_tag="ACG9BB_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838014.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6965..7543
                     /locus_tag="ACG9BB_002085"
     CDS             6965..7543
                     /locus_tag="ACG9BB_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFN2838015.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7638..7775
                     /locus_tag="ACG9BB_002086"
     CDS             7638..7775
                     /locus_tag="ACG9BB_002086"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838016.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8588..9091)
                     /locus_tag="ACG9BB_002087"
     CDS             complement(8588..9091)
                     /locus_tag="ACG9BB_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFN2838017.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9450..10475
                     /locus_tag="ACG9BB_002088"
     CDS             9450..10475
                     /locus_tag="ACG9BB_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN2838018.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10798..11676
                     /locus_tag="ACG9BB_002089"
                     /pseudo
     CDS             10798..11676
                     /locus_tag="ACG9BB_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            12152..12268
                     /locus_tag="ACG9BB_002090"
     CDS             12152..12268
                     /locus_tag="ACG9BB_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFN2838019.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13196..13534
                     /locus_tag="ACG9BB_002091"
     CDS             13196..13534
                     /locus_tag="ACG9BB_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838020.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13628..13858
                     /locus_tag="ACG9BB_002092"
     CDS             13628..13858
                     /locus_tag="ACG9BB_002092"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFN2838021.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         4437 a         2310 c         2671 g         4530 t
ORIGIN      
        1 agtagcttct acggaaataa gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa
       61 ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcttatttt gattagaact
      121 tgcgaaaata aaccggttaa agtgtgtcta gcgcaagaga tttgctataa tagacgataa
      181 gggtaaaatg taacgagaag gggttgaaaa atgtgtatca aattgtaaca gattcttgtt
      241 gtgatctgcc ataccatgta ttagcaaaag aaaaagttga ttttttatca atgaatctcc
      301 aattgaatga tcaagagtta gtagatgacc tcggaaagac attcaattat gaccgctaca
      361 tgaatcagct gaaaaatggt gcgatgccta cgacatcaca aatcaatgtt ggccgatatt
      421 tagaattttt ccgttcttat gcggaaaaac agattcctgt tttatatgtg gctttttcca
      481 gtggattaag cggctcttac agtagtgcgc ttcaagcggt tgaattgctg aaggaagaat
      541 atgaagaccc tgagatctat gtttttgata caaaagctgc aagtattggg gaagggcttt
      601 tagtattgga agctgtaaga ttgaaaaaag aaggtaaaag catcgaagaa accctagagt
      661 ggctggaaag taattatttg aaagtccatt catgggtaac agtagacgat ctgaaatatc
      721 tagaacgagg cggtaggatt tccaaagcaa gtgctactct tggcagtcta ttgagtgtaa
      781 agcctattat tacggtggat aaagaaggaa ggctgacgaa tgttgacaaa gttcgaggaa
      841 gaaacaagtc gatccagaca atcgtcaatg agacggtaaa aacaatcgtt gcaccacttg
      901 atcaaatcgt ttatgtggct tacgctgggg atctagagag cgcggagaag gcaaaacggc
      961 ttttggaaga gcagataaag atgaaagatg tgaagcttta tcccttagga ccaacgatcg
     1021 ccagtcacac aggctatggc tgtatcgcga ttttttcgat gggaatcagt cgataaaagc
     1081 agtaactcat tctgtaaaaa agcccagaaa ttgattgtaa caaaaacagc gactcttaaa
     1141 agatagatga gcatcctatc tcttagaggt cgctgttttt ttatttttta agctaaatag
     1201 gccattatgc ccataactcg ttcaattgtt tttgaatatc tgcattttcc aaaaattcat
     1261 catatgtcgt atcagctcga tcgatcacac ctttatccga aacggcaatg atgcggtttg
     1321 ccaatgtttg gataaattga tggtcatgtg acgcaaataa gattgagcca gtaaatgcca
     1381 tcaagccgtc attcaatgct gtgattgatt ctaagtctaa atggttcgtt ggatcatcta
     1441 atacaagtac gttagctttc gaaagcatca tttttgaaag catgcaacgg actttctctc
     1501 caccagataa aacattcact ggtttcaata cttcttcacc agaaaacaac attcttccta
     1561 agaagcttct taagaaggtg ttatcatctt cttctttact tgcatattga cgcaaccagt
     1621 ctaaaatcgt tagatcagta tcgaattctt tcgttgtatc ctttggtaaa taagaattac
     1681 ttgttgtcac accccaacgt actgttccag tatctggcgt aatttcaccc atgataactt
     1741 tgaatagcgt tgttgttgtt atatcatttt ctgcgacgaa agcgaccttg tcttctttgc
     1801 tcaaattaaa agatatatca tctaaaattt tctttccatc aatcgttaca cttacatttt
     1861 ctacctgcaa taagtcgttt ccaatctcac gttcaggttt gaatccaacg aatggataac
     1921 gtctagaaga aggttggata tcgtctaaag taattttttc aagcattttt ttacgtgaag
     1981 ttgcttgttt tgattttgat gcattcgcac taaagcgggc aataaattct tggagttctt
     2041 tgatttgttc ttcttttttg gcatttgcgt ttgcttgaag tttagccgct agttgactag
     2101 attccaacca aaaatcataa tttcctacat aaagtttgat tttaccaaaa tctagatccg
     2161 ccatatgtgt acatactttg ttcaaaaagt gacggtcatg ggatacaacg atcacagtat
     2221 tttcaaaatt gatcaaaaat tcttccagcc aattgatcga ttggatatcc agaccgtttg
     2281 ttggttcgtc tagcaaaaga acatcaggtt taccaaatag tgtttgtgct aataacactt
     2341 ttaccttttg tccaccagtt aattcactca tttgctgatg gtgcaattct tcagggatgt
     2401 tcagaccttg cagtaaaaca gctgcttctg gttcagcttc ccatccatta agctcagcaa
     2461 attctccttc taattcagct gctcgaatac catcttcgtc agtgaaatcc tctttcatgt
     2521 aaatcgcatc tttttctttc agcacttcat acagacgctt gtgtcccatg atgactgttt
     2581 caagtacagt atagtcttca tagtcgtaat ggttctgttt caatacggcc atccgttcat
     2641 ctggtcccat agtaacggaa cctgtggaag gctgcagatc ccctgataac actttaagga
     2701 acgttgattt acctgcacca tttgcaccaa tcaagccata gcagtttccg ggggtaaatt
     2761 taatattcac atcatcaaat agtttgcggt ctgaaaagtg caaactcaca tcatttacag
     2821 taatcaattt ttttcctcac ttttatcatt attagttttg acaagtctta tagaattata
     2881 taggggaaag gtaaaagatt caaggcaatc gaacattctt tgtgtttcac gtgaaacaga
     2941 atgaaaaaaa taaaaataaa agcggagcga aaatagctac tataggattt attagtttgc
     3001 aacagcataa tttttgacgt tatttataag agggagtact ataaaactgt aaaagaaatg
     3061 aaagaaggat tgaaaatgga tgaaaaaaga accatcgact ggggctcatt gattttggga
     3121 attttatttg tacttgtctc attgttatca tttcaagacc ctgtcggaaa tttagttgca
     3181 attgtagtag tctttgcgat ttttgccttt attaaaggga tatttgaact gtttgtacgg
     3241 aacagattaa aagagctaac aggatataaa ggaaagatgc ccttaataat cgggatcatc
     3301 gatattctag ttggcgtctt tttcttattc aatattggtg caggagtcgc agcattgcct
     3361 tttgtttttg cagtatggtt tattgctgat tcagttttag cgttatttac tgctgacttg
     3421 gcaagaggag taagtgaagg atattattgg tttacgatca tcgtcaatat tttaggaatt
     3481 ttattaggaa ttttcttatt gtttaatcca atttcttctg cgttgacatt aagcttttta
     3541 gttggattct attttatgtt atttggtatc acacatatcg tctatgcatt tagataagta
     3601 aaatcaaaca aataaaaaag ccggcttccg attacagttt tactgttgta attggaagcc
     3661 ggctttttta tcatggacaa atatgcacga ggtgattttt aggtgtcgag acaaatttat
     3721 gcaaaaatgt gagaagctat atacacaaat tttatcttaa tactcgaagc atagcgcagt
     3781 tgtcacaacc tcttttcata taattcattc aatactggca aatcatggac ttcatatttt
     3841 tctttgaatg cttggatttc ttcttcagaa tattttcttg gatctaaatg cagatcttct
     3901 actggcaatg ggcgcttgtt attgatcttc acatcgatta ctacaggacg atctgatttt
     3961 gcagctgcat caaaggcaaa tcgaagttgc gagtattctg tgactgtaaa gctatttgcc
     4021 ccgagcgctt ctccaacttt accgaaatct gcatcttcta aaaatacacc aaatttcttt
     4081 tgttccgtat cttcttgttc tgcttcgatg aaaccaaaag aatcattcga aaagacaaca
     4141 ttaataattg gaagctgata tttgacttgt gtaatgatat cttgcatgac catagcaaac
     4201 ccgccatcac cacttaaggt aaatacttgt ttttctgggt agcttagttg tgcggcaatt
     4261 cctcctggca caccattacc catagttgcg aaccatccag atgtcgtatg tttttgttta
     4321 ccgttcatat tcaataaacg aatagaatgg atagtcgtgt taccgacatc tgtgacgaaa
     4381 atcgcatcgt cttcagcaat acgattgatt tctttataga cagcctctgg acgaatcggt
     4441 tcttcctctc gatcttcaaa agatttcaac caatttaccc atttttcttt attttcttga
     4501 tttgctaggt accagctatc agcagggcga gcatccccta actcgacaag tctttgcaaa
     4561 gctgtgtttg catcaccgag gatcgacaaa gatgtttcat ggcgtcgtcc aaattttgac
     4621 gcatcgatat cgatttggat aaattcagca gttggattaa aaaatgcacg tccaaatggg
     4681 aagtcgctac cgacaaataa gatcagatcg gcttctgcca atgcttcatt tccgggttta
     4741 gtagcgacac gtccagcaaa accaagaaag ttttcataat catcaggaat aattcctttt
     4801 gctagaacgg aagcgacaac aggaactgaa aaatgttctg agaattcttt gatttgcgga
     4861 aatccgtttc gcgttccttg accaacataa agaactggtt tttttgcttt ttcaagataa
     4921 ggcaaagcag ctagcaaatc tttttcttct ggtaatatca cacttgtttt gtgagtgtga
     4981 gcggtggaga aaggttgttc gtcaatttct tcaaaaccaa gatcaacagg gatagtcacg
     5041 atagccacgc ctttatgctc ataggcggct ttaatggctt catctactac atgcggaaga
     5101 ctttgtggtg tcatgactgt acgattgtat acgctaacat ctgcgaaaat aggattttca
     5161 tttagttcct ggaaagagtt atagttcatg gatgtagaag caacttgtcc taagaacgct
     5221 actagaggca catggtccat ctgtgcatca tacaaaccat tgatcaaatg tgtcgcacca
     5281 ggtcctgcag agccgaagac agcgccgact ttccccgtta attttgcatc tgcagcggct
     5341 gcgagtgcgc cgacttcttc atgacgaact tgaatgtagt tgatttcggt tttttcttga
     5401 tatagcgcat ccataattga attgaaagat cctccgggaa tgccgtaaat atggtcgata
     5461 ccccagcttt caagaacttt taccattgcg attcctgcat tgatttttga catggtgttt
     5521 cactccttta gcaatgatga tacataaatt ttttgtatct agagtataga actttttatc
     5581 gaaaatccca actaattcgt cttttcacaa aaatgtagtc atgtttttgg gaatatgcta
     5641 gagtagtaca tagatagatt caagagaggg gaatggcatg gttgaatggc tattgagact
     5701 tgaaccttgg cagcaagcac ttgctgggac agcttttact tattttatga cagctcttgg
     5761 tgccgggttg gtttttttct ttaaagaaat caaaaaagaa gtgctaaact tgatgttagg
     5821 atttgcttct ggtgtcatga ttgctgcaag tttttggtct ttgttagatc cggctattac
     5881 aaaagcagaa gaaaatggag atattgcttg gctagttgta agtattgggt ttggtttagg
     5941 cggtgtgttt ttatatatgg cagataaaac actgccccat atgcattttg gaccacaaca
     6001 tgaagcagaa ggattgccta cccatttaaa acggacgatc ttgcttgttt tttctattac
     6061 attgcataac attcctgaag gtttagccgt gggggtagca ttcggtgcag cagcaactgc
     6121 ggataatccg acagccgcta tattagccgc tgtttcggtg gcgctaggta ttggtatcca
     6181 aaacttccca gaaggagcag ctgtatcaat cccgttgcga caagaaggtc tgagtcgtaa
     6241 aaaggccttt gtttacggac aagcttctgg aatcgttgaa ccaattgctg gagtgattgg
     6301 agcgctacta gtaacgaaag tggagctgct gcttccttat gcacttgctt ttgccgcagg
     6361 agcaatgatt tatgtcgtgg tggaagaatt gatccctgaa gcacagcaga cattgtccag
     6421 caaacgtcat tttgcagttt ttggcgtcat gagtggattt atcattatga tgattttgga
     6481 tgtggcatta ggataaataa aaaaaatata tatgatatat caaatagggc cgcgaactta
     6541 tatcgcgacc ctattttttt agtaagttat atcataatga agtgaatggt atcatacatt
     6601 aataatagtt ttttctatgt gtatttcctt tgatccagcg aacaagccag atgataagtg
     6661 cgattgggat tagaactttg accgcaaacc ataaaagccc gcttaggaca ctgacaaaaa
     6721 gactgatgac gaagctagct gcaattagca ctaaaagaac catgaagata tttaagaaca
     6781 tatttttttg acgcatacaa aaccctcctc ttgttcattc tttactatag aaaaagtata
     6841 acagggattg tttaaaaaca cctccaactg aagactgatt tttacttcat ctacttaaat
     6901 gatcaaacaa ttgctttttc tctggtttat catctaaaat agagcggaaa aggaggagaa
     6961 tatcatgcct aaaaatcgtc ttgaagcatt tacagatgct gtaatcgcga ttatcatgac
     7021 tattttagtt ttagaacttc atgaacctaa cggagacacg tgggaggcgc tcgctgcatt
     7081 aggttaccga ttttttatct atattgtcag ttttatcatt ttagcgattt attggaacaa
     7141 tcatcatcat ttatttcaag ttgtacataa gataaatgga cgggtcttat gggcgaacag
     7201 tttctttgtt tttgcgcttt cgctttttcc gtttgcaact tcatggatca gtgagtacat
     7261 caatagcctg gctccagaaa tgacttatgg ttttgttacc ttgaccgcta atattgcgta
     7321 ttatatcttg gcaaaagaac ttgtcaaagc aaataaaggc cacgagaaag cattgaaggc
     7381 gatgttgaat aattacaaga aatcgtatct atcgattggt ttgaatgtgt taggaattgt
     7441 cttagggtat ttagtagcgc cagtagccgt gctgatcgca aacatcgtga ttctagctgc
     7501 ttggatcatc ccaagtcgta ccatcgaatc acaatatcgg taaaatgatc cgtgtttaaa
     7561 atttaagaga aaaatataga aaaaataaat aaacgatcaa atatccgagt gatactagta
     7621 ttcaaagagg gatgcagatg gatcaaaaaa gaattcatgt aggactgatt actagttttg
     7681 tcacgatgct tatttatttg gatcaaggtc atttgacttg gacagtagtt ggtgtcgtag
     7741 gatttttgac attattcaca gcaataaaaa aataaacatc tttatgtaat ctgatgttct
     7801 atctatttaa gaaaagcatg tttacagcag tatgattctg ttgtaaacgt gtttttattt
     7861 gtggaaaatt taatttttag aaaagtgtat cgaaaaatag atagttagtt tttccacagt
     7921 ggataacttc gtaagagata aaaataaaaa attagtcaaa gaagggcgta tagatttctt
     7981 ttgataggag atatacaaca gaaatgaata aatattaacg tgttttcaaa cagtataaaa
     8041 cggtgtggtt tttggaataa aaaaataaca tatctcattt tacacttttg ttattttata
     8101 cttaaaaaat aacgacacag aaaataccgt gtctcagatt tctctactac ttctaaaaac
     8161 tactgttttt ttcctttgaa atgccttcaa aaagtaggat caggcagaat atactggcag
     8221 aatcaaggag aagggagcct aaataaggag aaaaaccata gaaaatataa agtaattact
     8281 tataaaacaa gctttttata gaaaaataat atacctgagt agggtataaa ttaagacgga
     8341 attcctttct agattgagat attgactagg aagaatgtta agattttaag cctttaagct
     8401 aaaaaaaaac cctttttgtt aaataagggg aatatagcct gtcagtttca aactgcaagt
     8461 cgtcctgctg caataatata gtagattaga gatgaaaata gattttttta aaccaatgac
     8521 tgaataaaaa gaccgtcata gatacacatt ggaatgtgtc tattacggtc tttttttacg
     8581 aacgatttta ttgaagacgg acagttcggg tcaaaaataa aaaagctacg atcagaaata
     8641 agaataaaat gtatcccaaa ataatggcta gacggtctaa cttatttgtc gatagatgtt
     8701 tgtttagaag ttgataggcc cataaaccta tcagtcctcc tgttgtatta gttatcaaat
     8761 cagtgatgtc cgctacgcca ataccgaata ggaattgaat caattctgcc aagaagctta
     8821 caaggaaaat aatccctaat tttttagaaa aagaaagtct cttgaaattg acattcaaaa
     8881 gcaccccaaa aggaaaaaag acaatcaagt tataagtggt ctcacgtaat accgtcgtat
     8941 tttcaaaaga aaaagtagaa aaagggacga gattgacatt gctgtaagaa taagtgagaa
     9001 attgtggaag attaaatgat agcttgaata aaatcaacca taatagaaag aataaataaa
     9061 tccctaataa aaattttgat acataccgca tagacatctc ctccatttaa aaagtatacc
     9121 atcgaaaacc gctgaaaaat cataaaaaag tatgaagttt aaggaccaac gtagttatcg
     9181 tacgaaaatt agaagctttt ttttgttggg aatccggttt ctttaacaaa gctaagatag
     9241 cgagttgctt ggaaaagttg ctatcttttt tctgtcaaaa cgactcggaa tgttcgtttt
     9301 ttgttctaat ttctccaagt tgtttacttt ttgatactaa gtttatgaaa aatcaagaaa
     9361 tagttgttat actaggtaat gctgacaaac gggattggca tttcatcttg gttcagcttt
     9421 ttttccaatt actaggaagg agggatagat tgttcaagac ttattttcgt ttcaataaaa
     9481 tacaagataa tccttttcgt atcatgaatc tgatgctact tatctttatt gtattatggt
     9541 taggttacat aaatcatgat tttttgattt caagtttttc atcattaggg atctttactt
     9601 ttttctatta tcaaaacata ccgatgaaat atttgctgaa acgtatgttt ctgatcggtt
     9661 gcggactgtt ggttgctttc gttttgggga tcttatcgac ttatgtttta tggcttgaac
     9721 catttgccgt agctttggtt gctttttcca gcagattcgt gttgcgatta ttccatattt
     9781 ctaaaccagg tggcttgttt tttgcgatgc tttctgccat gggaacgagc atgcagctgc
     9841 caatcgctca attaccgatc gtctcacttt atttttttat gggtgtcgtt tttgccctga
     9901 ttgcggcagt gattacaaaa ttactggatt cacggcctga gcagacaatt gaaaaagcaa
     9961 ctttaaaaga aagatttcac gaagagcctt tagtgatcat tgattctgtt ttctacagcg
    10021 ctgccttatt tctttccgtt tatgtcagtc acggcttgaa tctgcataat ccttattggt
    10081 tgaccttgtc atgtgcatcg attttgctag cagaaaactt ggatgcgatg aagcatcgac
    10141 aagtccaata tctgattgga tcgatgggtg ggttatgtgt gtcggcattc ttgtcatttg
    10201 tgccgtttac gcagttgcag accatttttc tgatcacttt tctatatgga atcgcacaat
    10261 ttctagttgc tagaaattat gcagttgcta atatttttct taatccgatg gcgttgatgc
    10321 tatctacact tattcgtgga gcatacttga tttctttgat cgaatatcgt tttttaggga
    10381 tcgtcatagg aagtttcatc ggtttaggtg ttgcgtgggt aatgacggtt ggtttgcaac
    10441 attatttaac ggttgttcgt caaaaattag aatagaagga caaatatatg tgccaaaaga
    10501 ggttatgaca agatttttgt cacaacctct aattcaaggt gttcaaaagc cagattctca
    10561 gcattaagtc aaattcacac aaaaatgtgg aaaggaactt tcaaaaaaat tttatattta
    10621 ttctatcaaa aaattttgtt tatgatggta actgaataaa ctttcctgag atggtcaatg
    10681 ctttccttga aagtcttaca gatattcttt cttcatagaa agtttaagtt ttatagttag
    10741 tgtacaataa aagtatgttt ataccatatg gtgtccggat aatcagtcat ttaaacaatg
    10801 aggttcaaag atatgaaaag gggaaatttg atgaagaaaa taagcaatga aatccaaatt
    10861 ctggcagaaa ctttaactct tcccaaagtc gttatagagc aaatgattgt acaagcaaaa
    10921 aacatccttg atgaggtgtt tgagcaatat agtcatgaac taatggatcc taataaagca
    10981 aaggcgactg caaagaaaat agacaaagtc tatgaagagg aaccttttgc agcattagct
    11041 atttatctat atgcagcttc tcaaagttgg aaaaaaattt atcaaccatt gaagattcca
    11101 agaactatct atttggcaac aatgaatgct tttactagat ttattcatga acatttccaa
    11161 gtaacaggaa gctatcgctt tgaccgtggt ttttggattt ggcattacac tagtggcttg
    11221 atctttcgga ttggtgaatt ggagtacgaa atgacatatt ttccgaaagg gtacacggat
    11281 ctaagattag aaggaaagca gtgcctttct cttcatattc cttccgatgc agatttatct
    11341 cagaataaga taagagatag ttacgaaagt gccattcgtt tttttgaact ttattttcct
    11401 gattaccatt accaagtgat gtacacagat acttggttac tatcacccaa tcttactaaa
    11461 tagttgaaaa aagagtcaaa aatctgtctt tttgcagccg attatcgatt attatcagtt
    11521 gatgaacagg acgatagtgg agtcccttgg atctttggac gagtagatgc ccaaatacat
    11581 gattatccag aaagtacgag tttacagcga caagcaaaag agcaattgct tgcaggcgag
    11641 cacattggct ctggcttagg tattcttact tcataagttt agtacttcga aatagtttaa
    11701 aagagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
    11761 cggaagcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt gaaaagcaat
    11821 tttcgtgaat ttcttcttat ttcttggagt taaacgcttc tgtcccagcc tcttttttag
    11881 tgttctatcg aacacacagt acgttcaata ggtaaactag ctataacaat agatattaaa
    11941 gttaacggac aagagggcct tttttaggta gctaaaacgt aataatgaaa atttatttcc
    12001 agagatagga gtcaatcagc tagtctgatc gactgtttta acaagttgac tttcatttta
    12061 acttttgata ccatttaaaa cacaaatcgt aattgtttcg atttgtgttt tttagcatga
    12121 ctacaatcaa agaagaaagt gggtcgtcca tatggaaaaa acagatatct caagtgcata
    12181 ccgccgcttg aaaagcccaa atatcaaaac acgaaaacgt gcgctgaaga tcatcaaaga
    12241 tgtgaaacgg aatagtggga aaaggtaaga agaaggaaac ttaattaaaa aaatagccga
    12301 aaaacgtttt tcggctattt tcaataatcg gtttttgtgt aaggtttccg aaagaactgc
    12361 tgttgatttt ccatttttaa catatgataa attggtagaa tctttctttt caaattgata
    12421 ccattcccta attccagtta tatagttaac tacagaacca caaagcacgt acgtttataa
    12481 aagtattatc tgtagttatt tttcaattcc ctttcaacct cttattttga ccagtagtat
    12541 tgatcacttt accttttaac acctttgttc gtttttgaaa catttttagt caagaatgga
    12601 aacgtaacta cataatcaaa ggttttgcga aatatttcat gaaatatttc atgtagaagc
    12661 gagcaatacg gttgcaaata accattatcg tttaataagc agttacgaaa gctcccaata
    12721 tctctaacgt tcagctcact ccatttcttt tcttcaaaaa aatcagtgat tcgtatctct
    12781 ttctatttgt cgaaaaagtt aactttaaaa agtaacgccg aaaaaatatg caaaaaaata
    12841 aaaaagcagt tactattgct agaataacat caaggaataa taagttttgg ttttcagata
    12901 atagtcgata agttgtgtat tggaatttta gatacagtaa taaagtattt ttcatgaaat
    12961 tttgtgagaa tcactattac aagtatcttt aataaaatta atggttaagg aggaacttaa
    13021 agtagaatgt aaatgaatag ttgtagtcag tcgattgtga tactagactc aaagttgcag
    13081 gaataagttt ttcaatttgt gattggaaga taaaaaatga aaaagaaggg ctttttttta
    13141 tactttgttg attgtagtgt ttattttccc tctgtttagc caagtaacat tttctatgac
    13201 tcaaccagtt tcaagtgagg aacatgataa cctttttcgt gctacagaag agtttaagaa
    13261 atatcaggaa aatattgttc cggagactcc attagctagt gagccaatta ctgatattga
    13321 tggaaaccaa ataggatggt atatacagta cgaagttatg gtggtaaata atgactcaat
    13381 agtaaaaaat acagtaggct tagagctaac accgattgaa aaggtgagtt cattgttaac
    13441 gtatatttat actgatgaaa gtgacgagac tgagatcatt ttaacggatt actcaaaaat
    13501 tgctactgat aaaaagtata tattgtggat gtagacacta gtgaaatact ttatgatata
    13561 gaaattgatg aaaatttact agatgtagca caagaaatcg atcatcaaaa gcaggaagct
    13621 attcgatatg ctggtagaaa agggaaaaga aaatatttat tacgtgaatg tagcaaaagt
    13681 tagagaagat gaaaatgagt ggaaagaatt caagagtcgc tactcaatta actcgactcc
    13741 gacatttaca gtttacagag aaggatcgat agaaaaaact gttttttgga ctaaagaaag
    13801 tggaatgtct ttagctgaag tagaagagtt tttggattat gttagtatgc aacagtaagg
    13861 gagttcttat tatgaaaaat tttgacacat attatgttat tgttttttcg atatctttgc
    13921 tgttaatatt actaacttta atgcagta
//
LOCUS       DAZAFF010000073        13936 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000073 DAZAFF010000000
VERSION     DAZAFF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.073"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..1111)
                     /locus_tag="ACG9BB_002093"
     CDS             complement(146..1111)
                     /locus_tag="ACG9BB_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFN2838022.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="ACG9BB_002094"
     CDS             1345..2190
                     /locus_tag="ACG9BB_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN2838023.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="ACG9BB_002095"
     CDS             2381..3568
                     /locus_tag="ACG9BB_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFN2838024.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="ACG9BB_002096"
     CDS             3574..3930
                     /locus_tag="ACG9BB_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFN2838025.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="ACG9BB_002097"
     CDS             3932..4270
                     /locus_tag="ACG9BB_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFN2838026.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4346..4602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4665..5699
                     /locus_tag="ACG9BB_002098"
     CDS             4665..5699
                     /locus_tag="ACG9BB_002098"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFN2838027.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="ACG9BB_002099"
     CDS             5692..6378
                     /locus_tag="ACG9BB_002099"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFN2838028.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="ACG9BB_002100"
     CDS             6402..7250
                     /locus_tag="ACG9BB_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFN2838029.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="ACG9BB_002101"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="ACG9BB_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFN2838030.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="ACG9BB_002102"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="ACG9BB_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFN2838031.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="ACG9BB_002103"
     CDS             9516..10751
                     /locus_tag="ACG9BB_002103"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFN2838032.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /gene="sufU"
                     /locus_tag="ACG9BB_002104"
     CDS             10738..11208
                     /gene="sufU"
                     /locus_tag="ACG9BB_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFN2838033.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="ACG9BB_002105"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="ACG9BB_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFN2838034.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(12702..13844)
                     /locus_tag="ACG9BB_002106"
     CDS             complement(12702..13844)
                     /locus_tag="ACG9BB_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2838035.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4536 a         2576 c         3008 g         3816 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tgggccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaacc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag ggtctgtagg
    12601 ttaataacta tttcattgct cgaaacgttg atattaaagc gtttcgagcg tttttttatt
    12661 tgtattttaa tggttggcta cgtttttggc tacgtttgga attattcgcc cataaatttt
    12721 gcaaatcggt caccagtttt cttaacagat tgtggtgtga catgagcata gatattcatg
    12781 gtagttttaa tatctttatg acctaatcgt tcctgtactt cttttatcga cgcaccactt
    12841 tcaaacaata agctacagtg ggtatgccta aatccatgtg gagtaatttg aggctccatc
    12901 ggtgtctttt tgtaaatcca gtctaaccaa tcgtttacta cttgggggta gtacaacttg
    12961 tttgtgtttg tagtgaaaag aaactgtttt ggtgaattcg tgttatagcc atataagatc
    13021 attattttca tttgttcttg ttgccaaata cgcagttgtt taagtgtaaa atcatcaagc
    13081 tgaatcgttc ttgatgaatt tttagtttta ggtacttgta gaactacttg attaaactcg
    13141 tcttgagcga tagttttacc aattgttatt gttttattga aatagtctat atcttcccat
    13201 tgaagcgcca atgcttcgct tttacgcatt cctgtataag ctaataaacg gaaaaaagtg
    13261 tacagtttaa ctgaattatt ttctttagta ttctgtaaaa acagttgtaa ttgatctttc
    13321 gtgtaaaact tcaagggttg ttcttcttca atttttcttg gaagcaatgt tttagacata
    13381 ggattatcct tacagacttc cattgctact ccaaatttaa atatttgagc agttgttttt
    13441 ctcaagaaac catattgttt taatgttttg tgccattcat tcacctgttt ttggcaatag
    13501 ctaacactta ttttatccaa tcgtaattcc ccaaaagcag gtaatatatg gtttttgcaa
    13561 aaacgttgag caacggcaac agaagacggt tttactcttg ttctgtagtt atcaatccat
    13621 aattcataca actcttgaaa agttgtaaca ttgattttaa actcttgttt ttctttaaag
    13681 tctacaagta gtctattaag tgctgtttgc gcctcagctt ttgatttgaa tcttctacgc
    13741 tttgtacgta tatcttttcc agtcttggga tctttcccta aataagtttc aaatccccaa
    13801 tatttttcgc cattttgtaa tttataacta aaaaatgttg ccatttgatt ctctccttac
    13861 ttcattattg caggtagaga agttatttga agaatcaatt atttttaaat ttatcgtgat
    13921 tatcagttaa aaattt
//
LOCUS       DAZAFF010000074        13890 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000074 DAZAFF010000000
VERSION     DAZAFF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.074"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..2208)
                     /gene="glyS"
                     /locus_tag="ACG9BB_002107"
     CDS             complement(133..2208)
                     /gene="glyS"
                     /locus_tag="ACG9BB_002107"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFN2838036.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2210..3127)
                     /gene="glyQ"
                     /locus_tag="ACG9BB_002108"
     CDS             complement(2210..3127)
                     /gene="glyQ"
                     /locus_tag="ACG9BB_002108"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFN2838037.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3511..4323)
                     /gene="recO"
                     /locus_tag="ACG9BB_002109"
     CDS             complement(3511..4323)
                     /gene="recO"
                     /locus_tag="ACG9BB_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFN2838038.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4466..5365)
                     /gene="era"
                     /locus_tag="ACG9BB_002110"
     CDS             complement(4466..5365)
                     /gene="era"
                     /locus_tag="ACG9BB_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFN2838039.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5380..5775)
                     /locus_tag="ACG9BB_002111"
     CDS             complement(5380..5775)
                     /locus_tag="ACG9BB_002111"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFN2838040.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5759..6235)
                     /gene="ybeY"
                     /locus_tag="ACG9BB_002112"
     CDS             complement(5759..6235)
                     /gene="ybeY"
                     /locus_tag="ACG9BB_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFN2838041.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6252..8444)
                     /locus_tag="ACG9BB_002113"
     CDS             complement(6252..8444)
                     /locus_tag="ACG9BB_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFN2838042.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8468..9439)
                     /locus_tag="ACG9BB_002114"
     CDS             complement(8468..9439)
                     /locus_tag="ACG9BB_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFN2838043.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10145..11524
                     /gene="brnQ"
                     /locus_tag="ACG9BB_002115"
     CDS             10145..11524
                     /gene="brnQ"
                     /locus_tag="ACG9BB_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFN2838044.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(11678..12124)
                     /locus_tag="ACG9BB_002116"
     CDS             complement(11678..12124)
                     /locus_tag="ACG9BB_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFN2838045.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12151..12327)
                     /gene="rpsU"
                     /locus_tag="ACG9BB_002117"
     CDS             complement(12151..12327)
                     /gene="rpsU"
                     /locus_tag="ACG9BB_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFN2838046.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12489..12917)
                     /locus_tag="ACG9BB_002118"
     CDS             complement(12489..12917)
                     /locus_tag="ACG9BB_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN2838047.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13009..13869
                     /locus_tag="ACG9BB_002119"
     CDS             13009..13869
                     /locus_tag="ACG9BB_002119"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFN2838048.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
BASE COUNT         3822 a         2881 c         2498 g         4689 t
ORIGIN      
        1 aaaagttaca caaaaaataa aataacaaaa aaattaacat tgaattaata atagttattt
       61 atatttataa caaataaacc agagaaatag cctattttgt gcctgttctc tggtttattt
      121 acatattaga aattattttg tgatgatttg atctaaactt gctagtgaca aagccatggc
      181 agcaatttga cttaattgcg ttaatcggtt cttacgtact gctggttctt ctgccatgac
      241 catcgtctga tcaaaataat tttcgatcag tgggcgaagg cttacaagct gatcataatt
      301 ttcgttgatc gtttggtcag caaagcttgt tttcattgcc tctactgctt tgtgcagttc
      361 tttctccgct tcgttttcga acagttcagg gtctacttct gattgttttt caagctccgc
      421 ttttttcgct aagttcacga cacgtgtcaa agcttccatc gatggtttga agtcttcatc
      481 tgcaagatgc tgcttgaaca attgtgctgc tgcaaatact tttgtcagat ctttttgttc
      541 tgcattcaaa acagcttcga tgacatcatg acggatattc ttcgttgtca ataattggcg
      601 cagacggcct ttgataaagt cgatcacctg ttgttgtccc gctgacaagc caatgccaaa
      661 acgatccgta tctttactga taacttcact aatcgtttct tgcagaacag acaaaggaaa
      721 ggcccatttt ttcgattcga tgattcgaac gacaccatat gcttgacggc gcaaagcata
      781 tggatcgttt gagccacttg ggatcatccc aactgcaaag aaagaaaaga tactatctaa
      841 tttatcagca atcgctaaaa cagaacctac tgtagtctca ggtaaatctc cttcactgga
      901 tataggcatg taatgttcac ggatcgctgt tgctacggct ggtttctcgc ctttaagcag
      961 agcatatttt tcgcccatga tcccttgtag ttcagggaat tctcctacca tgttggttac
     1021 tagatcaaat ttatagattt cagcagcgcg ttgcagatcc atcaattctt cctcagatag
     1081 tcctacttgt tcgcctatga tgcgagcgat gacttgtacc cgttgcattt tctcatagat
     1141 gctaccgatt ttttcatgga aagtaacagt ttttaatttt tcaacgtatt ggtcgattga
     1201 agctttcttg tcttcattat aaaagaattc cgcatcttct aaacgagcag tcaatacttt
     1261 ttgattgcct cgtaccacat tttccaattt tacccgatcg ccattacgag cagaaataaa
     1321 atgaggaagc aacatacctt gatcgttccg tacttcgaaa taacgttgat gttctttcat
     1381 agaagtgact agtacttctt caggaacaga tagatatttt tcttcgaagt cgccagaaaa
     1441 gacagttgga tattcgacta aattgttgac ttcttctagt aaatcttcat ctagatcaat
     1501 gatccagtga tttttttctg ctaattcgtt tgcttgtgtg atgatcattt cttttctttt
     1561 ttgcggtgaa gcaataacaa attgttcatt caatttttct tcgtattgtt ttgcatgcgt
     1621 aaatgtcaca tcttcgccta agaaacgatg acctcttgaa gctctgtctg ttttgacatc
     1681 caagatctca aacgggatta tttcgtcatc taacaatgcc acgatccagt gaatagggcg
     1741 aatatattca aaatcgtatt ctgcccagtg catcgttact gggaaagtga ggcttttaat
     1801 gacatctgct aaacctgtca atacttcttt tgcttctttg ccgtgagtgt atttcgttac
     1861 atagacatat tccacgcctt ttaattctct aaaagtgata gcatctgtcg tcagaccttg
     1921 tccgcggaca aatccttcag ctgctttcgt ccagtttcca tctgcgtcca aagcaatttt
     1981 tttggctggt cctttgactt cttcttcgct gtctgcttga cgttctggaa tgtttgttac
     2041 tttgattgct aagcggcgcg gtgtagaaaa cgtttcgatc gtatcataag tcaattggtt
     2101 ttcatctaaa aattttgctg ttttttcttc taattgtttc atgcttggtg tgactacatg
     2161 agcaggtatt tcttccagcc cgatttcaag taataagtct tttgccatct tattcagcct
     2221 ccttcacgtt ttctggattt gctgtgtttt tattcaataa agggaatcct aatttttcgc
     2281 gttctgccac aaagattttg gctacagcac gagccatgtt gcggatacgg gaaagataac
     2341 ctgcacgctc tgttacggat actgcaccgc gagcatctaa taaattgaat gtatggctac
     2401 atttcaaaat ataatcatac gctggatgaa caagattttc atcgatacag cgtttagctt
     2461 ctttttcaaa acgatcaaaa ttgcttagta gcaggtcttg gtcgcttact tcaaaagaat
     2521 atttagaatg ttcatattct ggttgtttga agatctcacc atatttcacg ccatcagccc
     2581 attccagatc ataaacactt tcaacttctt ggatataaga agcaagacgt tctagtccat
     2641 aagtgatctc agaagtaact ggtttacaag gtagtcctcc gacttgctgg aagtatgtga
     2701 attgtgtgat ctccatccca tcaagccata cttcccagcc cacaccggca caacccatcg
     2761 atgggttctc ccagttgtct tcaacaaagc gaatatcgtg ttctaatgga tcaattccta
     2821 aaagacgcaa gctttctaaa taaagctctt ggatattttc aggtgaaggt ttcattacga
     2881 cttgaaattg atgatgctga tacaaacggt ttgggttttc tccataacgc ccgtcagctg
     2941 gtcggcggga tggctctaca taagcagcat tccatggttc cggtccgatt gcccgtaaga
     3001 atgtatatgg gctcatcgta cctgctcctt tttctgtatc ataagcttgc atcagcatgc
     3061 agccgtttga tgaccaaaat ttttgtaatg ttaaaatcat atcttggacg gtcaattttt
     3121 tactcatctg ttttgcccct ttcaattttt aagcgttgct gtcaataaga atacaggaaa
     3181 gaaaacgaca aaaagtccct atgtctgatt acagacatag ggacgaataa tttcgcggtt
     3241 ccaccctact tccgaataaa gattcggcag cttcatttat cgtactcgca agtgcctctt
     3301 caaaaatcgt ttgggtccgg cttccactct cccgaactcg ctaaaccacg tgttttttac
     3361 tgatcttggt caatgtacgt attcattttg cagttttctg gtttgtataa tagatcctct
     3421 cagtgaacga tctatttaat ctacggttaa taatagacgg aagtccctgt atttgtcaaa
     3481 tagttttgat ttttttgttg tatctttcat ttattcttct ttttcaggta tctctttttc
     3541 aggcatattt ttctctggcg tctttaatac gtgtgcccaa gacttcattt gatcaatgaa
     3601 ctttttgctt tttagatgaa taccgacata ttcatcatac aactggtcaa tgaggtcacg
     3661 gattgcctgc ttcgtttctt ctttcaagtg gattccactg atcttatcat aagaaatagc
     3721 cgaaaacatt cgaacgaaat aaacagcgcg aggatctgca tgataacggt gttcgtccat
     3781 tggccagtgt ttctcgcata tcacaccgcc atatttggaa gaatagtcaa attttccttt
     3841 tgtttccccg catacgacac agtgcgtcca aattggcgcg attccgaagc gttgcagtat
     3901 ttgcacttca aagatattcg tgatgatttc tgcatcatct ccttggtcca tccgctgcag
     3961 agcttgttgc gtaaattgga ataaatgcgg gtcatattga tgatcttcga ttgctgcatc
     4021 tactagattt agcaaatacg taccataacc gctcaaaaag atatcttctt ggatgttccg
     4081 aaacgcatgg acgtctttga cactgcttaa aaacgacaaa ccttcacttc gaaaattacc
     4141 gatatagact gcttcggtaa aggggagcaa agccggagct aatggctgat tttgccgatg
     4201 gattcctttg atataaaaca tcattttgcc agaagattcc gtaaagattt tgactaattt
     4261 atctttttct ttgtaattac ggctagataa aatcaagcct ttcgattctg caagctggct
     4321 catattctcc ctcctaaaaa ataaatctgg aaaagcagat tctgccgatc cagattattt
     4381 atgtttcgat cttccgctgt ttttctcaac gtacggattt ttatccagtt tatttctata
     4441 cctaacctat aagaagtcca gaatatcagt actcgtcttt tcggtagccg aagtcttgca
     4501 gatagacttt tttgtctcgc cagtcttttt ggactttgac ccaaagttct agaaatactt
     4561 tgtctcctaa aagattttcg atatcctttc ttgctttcgt tcctacttgc ttcagcattt
     4621 ttccgccttt gccgataatg attccttttt ggctatctct ttcgacgatg atcgtggcct
     4681 ggatatggat tttatcattc tcgtttcgct tcatcgaatc aacaacgact gcgacagaat
     4741 gaggcacttc gtctcgtgtt aaaaacaaga ctttctcacg gatcaattca gaaacgatga
     4801 agtattcagg atgatcagtc acttgatcat ctggaaaata ttgtgggccc tctggcattt
     4861 cactgactaa tacatccatt aagcgttcga cattgtttcc ttctgtagca gagatcggaa
     4921 tcacttgtgc aaaaggcatc tgcgtcgaat aatcttcgat gattcccagc aactcgtctg
     4981 gatggatctt atcgatttta ttgataatca gataaacagg agaatgcacg ttttttagac
     5041 gttcaataat aaaatcgtct ccccggccac gtttttgatc agcactgatc ataaataaga
     5101 ctgcatccac ttctttcaac gcactgtaag ctgcttctac cataaaatct cctagacgat
     5161 gctttggttt atggattcct ggtgtatcga taaaaatcag ctgagcttct ggtgtagtat
     5221 atactccttg gattttattt ctagttgtct gtgccttgtc actcataatg gcaatttttt
     5281 gacccacgat tcggttcaat aatgtggatt ttcctacatt cgggcggccg acgattgcga
     5341 caaaccctga tttatgttct ggcatatacg ttctcctttt taattaaaca acaaatgata
     5401 tattttaggg acaaataaaa tcaacccaat gatgacagca aaacaagcgg tcactaaaac
     5461 tgctccagct gccatatctt tgattttttt cccaatcgga tgaaaatgaa aatccgtaaa
     5521 catgtccact acattttcaa aaacagtatt gatgatctct actatccaaa cgagaaaaac
     5581 agctaaaagc agccataacc actctatccg atcaagctgg aaaatcacgc ccataaataa
     5641 agcaaggata ccgaacgcaa catgtttcct catattgcgt tcttcctcaa aaacggtttt
     5701 gactccttgg acagcaaatt ccacagacat caggaaatgt ttgtttttct ctacgttttt
     5761 atcttttgag tccataggca tccaagatct ctttctgtaa accaaacatt tctttctcat
     5821 cttctggtgt catatggtca tacccgttga tatgcaagaa tccatgtacc gctaaaaagc
     5881 caagttcccg atcatatgaa tggccgtaag cttctgcttg ttcagcagca cgttcaatag
     5941 agatcatgat gtctcctagt acacgaggta atggattctc tccatcttca aaaatcactg
     6001 ggatttcgtc ttcgccttct tcttccagag caaaactgat gacgtctgtc ggtgcatctt
     6061 ttccacggta atcacgattg attacttgga ttgccgcatt gtccatgaaa gtaacagaca
     6121 tttctgtttc ctctggcagc tttaaataag atgctgcaaa ttgcagcaag tcatcgattt
     6181 ctttcatttt ctcttctgag acttggtttg tttcatccat aaaggtaata tccattatac
     6241 atcgcttctt tctattcttc ttgttcttcc tttatcttct caacttctgt tttcatttta
     6301 ggatatttga tccgagaatg gaaggtgcta cttaaaccgc tgattaatga tttttccact
     6361 tttcggattt ctttcaaggt cagtccacta tcgtctaatt gaccatcgga aatacgctct
     6421 tcgataagat tatggacaaa actttcgatt ttttcgctcg tcggatgatc cattgcccgt
     6481 actgcagcct cacaactgtc agcaatattg acgatccctg cttcacgact ttgcggtttt
     6541 ggtccaggat aacggaattg ctcttctgtt acttccggat tgcgttcttt cgctttgatg
     6601 tagaaataac gcattagcgt ggttccatga tgctgtttgc aaatatcgat caccatttgc
     6661 ggcatattat aatcttccaa tattttggct ccatctgtca catgtccaaa gataatatgt
     6721 ttactgtctt ctggcaataa gaaattgtgc gggttttccg ctcctgaagg caagttttca
     6781 acgaaaaagc tagcatgttt gatcttgccg atatcatgat aatagcaagc tactcgagtt
     6841 agcaaagaac gtccgccgat ttctgctaca gcgttagcac ttagattagc taccatcata
     6901 ctatgatgat aagtacctgg cgcttcttcc agcaactgtt tcaataaagg atgatttgga
     6961 ttgctgagtt catttaatac aattacactg tcatcattga ccatcaattc aatatatggg
     7021 tgtagaccga tcgtcagcaa gaatgagaag agtgcaccag tgaacccgca gacgagcatt
     7081 aaccaagtcg ttgcatcacc aaagctcatt ccttgataga cgatcaaaat gacatccatc
     7141 atcacaggaa aaataatgac ccacatggca gcagataatc cttgttctga gatccgtttg
     7201 cgtctgacaa tcgttgccat gagcccagag aaaacataag acatcaagat aatcgttaga
     7261 aagttcgtgc caatcgaatc ataaaaaatg aaaagtgcgg atactgcttg gaaaatacca
     7321 gccatgatcc ctgctcttcg attcaagaaa aagcttaaaa caagtggtgc aaaagcagcg
     7381 ggataaaaca gcgggatata agtagcttgt tccgtttgga ataattggaa aaatttcatc
     7441 agcactatgc tgactgacat agctgtcaca tagaaaagca cgtattttgt tctttctcct
     7501 gcattttgga attgaagtga attataaatc agtacgccga tttgaagcaa aacagctaaa
     7561 accatggcaa ttagcggaaa aatcgaagtt gtttgattag tcagtcctag taattccaat
     7621 tttttcatag ctgaagcatc gatttgatta ccttcacgaa caatgatttc tccttggaag
     7681 atcatcactg gttgtacagc ttctcgtgcg gattgtttca attcttctgt ctttttctca
     7741 ttcaatacgt cattcactac gataccttga tccactaagt agcgaatcat ttgttgttgt
     7801 gctggtgtga cattcaaata ttggatttgc tcttctgctt cttgtttaaa agcttccaat
     7861 tcactttcac gaacttgttt gctcatctgt tcgtcaatga gcttcagact ctcatccctc
     7921 acagtatgaa tctcagtttc agtcatttca aatatcgtcg aataaaaatt gttcggtagt
     7981 ttttgataaa acacaacatt ttctgcattc acattttcaa aatttttctt cagcgcggcg
     8041 atccgctctt ctgtagtagg ctttgggata ttttcatctt cctttgcctt atcaatcttt
     8101 tcgtcataag atttgttgat ggcatcattc gtcttatcga tcaattcaaa tagttgcttg
     8161 atccgattgt tctggtcatc tgcaagatct ttttggtaag tgtattctgg agtgactgct
     8221 tctgctgcca gttttctttt ttgttcggtt tcttccgtgt tctcgatcgt tttgttcgca
     8281 cggatacttt cttctgctac ttggccttct ctgaaatcat tggccttttg tttgacacta
     8341 ctaaatgtaa cgccgcagat aatgataaaa aacaagaaca gcaggaaagg caatgctgcc
     8401 tttccaagtt tttcttgtag tttctgcagt gttttattca acatgtgctt atcctcctaa
     8461 gcaaagctta ttcttgatgg agatctgcct tttcataagc tttgatgatt tcggcaacta
     8521 cagggtgccg aacaacgtca cttgcttcaa aattaacaaa atcgattttc ttgatttgct
     8581 ttaatgttcg ttctgcatga acaagaccac ttgtcgtccc tcgaggcaag tctatctgac
     8641 ttgtatcccc attgacaatc atttttgatt gataaccgag acgggtcaag aacattttca
     8701 tctgagcgac tgtcgtgttt tgtgcttcgt ccaagataac gaatgcatca tccaatgttc
     8761 gcccccgcat gtaagctaaa ggtgcgattt cgattacgcc tcgttccatc aatcggttcg
     8821 tatgatccaa tccaaaaatt tggtataaag catcataaac aggccgcaga taaggatcca
     8881 ctttttcttt tagatccccg ggaagaaagc ctagattttc acctgcttcc acagcaggcc
     8941 gcgtcaaaat gattttttgc acttcacctt ttttcaaggc agcgattgct aatactactg
     9001 caagaaaagt ttttcctgta ccggctggtc ctacgccaaa tacgatatca tgtttggcta
     9061 ccgattcgac atatttcttt tggccgctgt tttttacccg tataggtttt ccattacggt
     9121 ctttgacgat ctcttcctca tacatctcaa caaaataatc taatgtacct ttttgcccca
     9181 ttttcaatgc cgtcacaaca tcgggcgtac ttacgtggat tcctcgtttg atcaattcct
     9241 gtaatgtccg aatgactgag gcagccaagg aaatctggga ttgctcacca atgatctgaa
     9301 tgatttctcc acgtgtatgg atggttgttt ccgttgtttc ctcaatcagt tttatatgct
     9361 tgtcgtgtgc acctaaaagc atgctgacat catcagcagc cgtcaatcta atctctaaag
     9421 aactagactc ttccgtcaaa aatagacatc cccttttttt cgattcattc tttcctattt
     9481 taccatactc agaagaaact atatagcaaa taagaaaagg ttctaaaaga gttgcttaac
     9541 cataagtacc aagaaacgtt aaaaaactag ccgcttccat tctctaaagg tctcgtttga
     9601 ataaaaaaag ctgatcatgt aagctatgca taagatagag gacaagtatt ccgtctaact
     9661 tagcaagtat tctctgggta tttttcttgt ttcaaaaaaa gaaaacaaaa tatttgaaat
     9721 attcttgcat ttcaaaaaaa acaggtgtat tataacgtca acaaataaat atacagcaaa
     9781 gcgtcgagga aaacagtagg ctattcagtg atttattccc agtgagtcat gtaagtgaga
     9841 aatgacaata aatcgaatag acgaacatca tttccgagtc aatgtcgaaa acgtagatca
     9901 ttgtggctgc ctccattatc aggctagcgg ataactttat cgaaaagcgt ccgtgaatga
     9961 agaagctttt ttaagcgaaa aaaggtggta ccgttctaga atctagagcc cttttcccaa
    10021 gtggaaaagg gcttttttta tattttaagg tggtggtttt catggaatct gatattagtg
    10081 gtatttctag tttctgcaac gtttattttg ttactcaaaa tgataattat tggaggaaaa
    10141 atttatggaa gaaacaaaaa cttattcatg gaagcaatta ttgatggtca gttcacttat
    10201 cttcgggatg ttcttcgggt caggtaatct gatctttcct gtacacttag gtcaaatggc
    10261 tggatcaaac tggatcagcg ctggtgtagg atttgccatc tcaggcgctc ttttcccatt
    10321 attagctatc ttagctgtag tagtgacaaa aagtgatggt ctgtatgact tagctaaacc
    10381 tgtcggaaaa tggtatgctg ccttgttctt gatccttgtt catttaacga tcggaccatt
    10441 ctttggtaca ccgcggacag cagcaactgc ttttgaaatg gctgctaaac cctttttgcc
    10501 tgaaaaatat gattcattag gtatgttttt attctctgct cttttctttt tacttgctta
    10561 tttccttact gtacatccaa cacgtttggt caaatatgtc ggaaagtatt taaactcctt
    10621 gtttcttgca ttattagcta tcatttttgt cgtttcttta ttccaaccca tggggaatct
    10681 aaaccaatct gctgcagatt cttatcagac gaacgctttg acaaatggcg ttttagaagg
    10741 atacaacacg gttgatgcag tagctttact tgctttaagt gtcacttttg tccatgcagt
    10801 tcgtgatctt ggttttaaag ataaaaaagt cacagaatta acagcaaaag ctggtagcct
    10861 gagcgttgcg ttagaagtca tgatttattt tggtctggtc cttctcggtg cgatgagttt
    10921 gaataagatg tctttatcag aaaatggcgg aactgcactt tctcagatca ctaattttta
    10981 tttaggtaac ttcggtgcta gcttcttagg ggtaatggtc acattaggtg tcttcactac
    11041 tgctatggga ctcgttgttt cttttgcaca agatttccat aaactgttcc ctaaagtcag
    11101 ttatatgaca tggctgcggt taacgacttt tgtctctttt gtcgttgcga atgccggatt
    11161 ggataatatc atccaatggt ctttaccagt attgatgctt ttgtatccat tgtcacttgc
    11221 gttaatctta ctttccttaa cagcaaaatt cttccaaaag agtccatttg tctatcaagt
    11281 aacgatgttc ttttctgccg ttccagccgt attggatatg ttggcaaatt cgcctgcatt
    11341 agtgagtcag caaactgtcg tagcaagctt actaggattt tatcatcatt atgttccttt
    11401 tgctgcatta ggtcttggct gggtcgtccc aacgttgctt ggttacgctg gcagcttgct
    11461 cttttattat gtatatcgcg tatcaggata taaacaagaa gcaagtgagt taccagaaga
    11521 ataaccaaat caaacacaat ttaacataag agatattaaa caggaaaaaa acagctagaa
    11581 gaaagaataa gattctttct tctagctgtt ttttagttct ttttttacga acacaaaaaa
    11641 ctgcggataa tcactgtccg cagtttaaat gataaaatta agattgtaat agttctttca
    11701 caatcgcatt cacttggttt ccatctgctt ttcctttgac tttaggcata attgcaccca
    11761 taactttgcc aaattctttc attgatgaag caccagtttg agtgattgct tcttgaacaa
    11821 gttgtcgaat ttcttcgtcg gaaagttgtt ccggcatata tttttcgact attacaattt
    11881 cactttttac tttttcagct aaatcgtcac gtcctgcttc ttcaaactca tgcaaagaat
    11941 cacgtcgctg tttcatttca cgagacaaca ccgttagttc ttcttcttct gtcaagtcgt
    12001 gaccttcttt aatctgctcg ttttgtatag atgacttgat cattcggatg acttgtaaag
    12061 attccttatc tttcgctttc atcgctgctt tcatgtcatc gtttaatcta cttagtagtg
    12121 acacactacc aactccaatt catcacagaa ctagaatttt ttgcgttttc tagctgcttc
    12181 agattttttc ttgcgtctta cacttggttt ttcgtagaat tcacgtttac gagattcttg
    12241 taaagtaccc gcttttgaca cggaacgttt gaagcgacga agagcatcgt caagtgattc
    12301 gtttttgcga acgactgttt ttgacatgtt aattccctcc ctccgagctt aaaaagtaac
    12361 cgtaattgtt attgaaatat atctcaaaaa acaagactgt cctgtaaata ttatatctaa
    12421 tgctgttttg accgtcaagg aaatcttgct tttttttaaa attatcaaat gttattttat
    12481 ttaaatcctt attgacaatt ttcacatctt ccgaaaatct cgaatctatg cccttcgatc
    12541 gtgcatccac ctagctgttc ttcgaaataa ttcatcggac acatatggat ttctttcgtt
    12601 gttccgcaca ctgtacagat aaaatgatgg tgatggccga ttttcccctg acagcaatga
    12661 aaacgaaact tcatttctcc atttaaatcg gtttcttcca gtaaatcgat ctctgcaaaa
    12721 tcatggagat tccggtaaat cgtatcataa cttacccctt tgaattcctg attcatatag
    12781 tcaaaaactt ctttagcaga tacgtagcgg ttacgcttca ctaaataagt taacaatgct
    12841 tctctttttt tagtatattt aaaaccgtta tttttcaatg tttctaatga ttgttcgatc
    12901 aatgtctgtt ttttcatgac ttattcctcc aaatcaaaat gattacgaag tacgcattct
    12961 agagtatggt ataataaaca taaaaaaaat gcaagtgagg ttttatccat gtcttccgat
    13021 tctacagaaa tcgttacttt ttttgtcatc tccgactctg ctggagaaac tgcgacaaaa
    13081 ctagcgcaag ctactatggc tcagtatcct accgttgagt tcaacctatt ccgcagaacg
    13141 ttcgtgacag atgaaaaaac attaaggcag gcattgcaag atgcattaga agaaaaagca
    13201 cttgtcctgc atacattaat caatcaagaa ctgatcgaga taacacgttc tttttgtgaa
    13261 gaacatcaat tatttagttt tgatgtgatg acgccaccga tatcagaaat tgaacgttta
    13321 actggtatca agccaattcg gcaacctggt gccctccatt tattgaatga gaactatttc
    13381 aaacggatca aagcaatgga atttgctgta aaatacgatg atgggaaaga tcctcgtggt
    13441 tttttagaag cagatgtcgt tttgctaggc gtatcccgaa cttccaaaac gcctttgagc
    13501 ttgtttttag ctaacaaaaa tttaaaagtt gccaatttac ctttgatccc acaagcacat
    13561 attcccaaac agctttggga gatcgatcct aaaaaaatcg tgggactaac gaataaccca
    13621 gatattttaa ataatatccg taaagaacgg atgcgctctt acggattaaa ccctgatact
    13681 gcttattccg atatcgaaaa aattagagct gaacttgatt ttgcgaatga tctttatgaa
    13741 aaactaggat gtgtagtgat caatgtggca tctttatcta ttgaagaaac ggcttcgctt
    13801 attttgaatg ctttagacct agaagatcat agttattatg gaactgaaac aacagaaaaa
    13861 agcgattaat catttttgtc tttttcttaa
//
LOCUS       DAZAFF010000075        13675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000075 DAZAFF010000000
VERSION     DAZAFF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.075"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..317
                     /gene="yaaA"
                     /locus_tag="ACG9BB_002120"
     CDS             75..317
                     /gene="yaaA"
                     /locus_tag="ACG9BB_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFN2838049.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            304..1428
                     /gene="recF"
                     /locus_tag="ACG9BB_002121"
     CDS             304..1428
                     /gene="recF"
                     /locus_tag="ACG9BB_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFN2838050.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1425..3371
                     /gene="gyrB"
                     /locus_tag="ACG9BB_002122"
     CDS             1425..3371
                     /gene="gyrB"
                     /locus_tag="ACG9BB_002122"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFN2838051.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3391..5862
                     /gene="gyrA"
                     /locus_tag="ACG9BB_002123"
     CDS             3391..5862
                     /gene="gyrA"
                     /locus_tag="ACG9BB_002123"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFN2838052.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6098..6397
                     /gene="rpsF"
                     /locus_tag="ACG9BB_002124"
     CDS             6098..6397
                     /gene="rpsF"
                     /locus_tag="ACG9BB_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFN2838053.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6447..6959
                     /gene="ssb"
                     /locus_tag="ACG9BB_002125"
     CDS             6447..6959
                     /gene="ssb"
                     /locus_tag="ACG9BB_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFN2838054.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6985..7221
                     /gene="rpsR"
                     /locus_tag="ACG9BB_002126"
     CDS             6985..7221
                     /gene="rpsR"
                     /locus_tag="ACG9BB_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFN2838055.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7364..9337
                     /locus_tag="ACG9BB_002127"
     CDS             7364..9337
                     /locus_tag="ACG9BB_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFN2838056.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9343..9795
                     /gene="rplI"
                     /locus_tag="ACG9BB_002128"
     CDS             9343..9795
                     /gene="rplI"
                     /locus_tag="ACG9BB_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFN2838057.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10069..11436
                     /gene="dnaB"
                     /locus_tag="ACG9BB_002129"
     CDS             10069..11436
                     /gene="dnaB"
                     /locus_tag="ACG9BB_002129"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFN2838058.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12216..13508
                     /locus_tag="ACG9BB_002130"
     CDS             12216..13508
                     /locus_tag="ACG9BB_002130"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFN2838059.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4561 a         2469 c         3012 g         3633 t
ORIGIN      
        1 ggataaattt gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa
       61 atgagggatg ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc
      121 aaatgttgaa agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag
      181 aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg
      241 gcatgatggt tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg
      301 ataatgaggc tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc
      361 gaattttcca agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt
      421 ttggaaagta tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa
      481 ttgattggct ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc
      541 actcttccat tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc
      601 gaacagaaaa agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa
      661 gatctttctt tagtaaaagg cagcccgcag atcaggcgga agtttttaga tatggaatta
      721 ggccagatca atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa
      781 agaaaccagt acttgaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat
      841 attttgtccg aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc
      901 gtcaagcgat tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag
      961 cagctggaaa tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa
     1021 atccaagaac aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga
     1081 acaacgacag ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg
     1141 cagacttatg gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa
     1201 atcgatttga ttaaagaaga gacaggggaa tatccaatcc ttttgttaga tgatgtgatg
     1261 agcgagctgg atgataatcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa
     1321 acgttcttaa caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag
     1381 attttttatg ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa
     1441 gtttagtaga acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg
     1501 aagccgttcg taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc
     1561 accttgtttg ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga
     1621 aaatccatgt gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga
     1681 tacctgtaga tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt
     1741 tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag
     1801 taggatcttc tgttgtaaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag
     1861 gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg
     1921 aacaaacgga tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag
     1981 agacaactga gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga
     2041 accgcgggat gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat
     2101 accattatga cggcgggatc aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga
     2161 tttttccaga acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta
     2221 tgcaatatac agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt
     2281 atgaaggtgg aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt
     2341 atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc
     2401 gagaaggatt gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa
     2461 caaaaacaaa attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac
     2521 atttcatgaa attcttgctg gaaaacccaa gtgtgggccg gcaaattgtt gaaaaaggat
     2581 tactagcttc cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag
     2641 gtgctttaga gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg
     2701 aaaaatgcga attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc
     2761 gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag
     2821 caagcatgga caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta
     2881 cagggtttgg agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga
     2941 ctgatgccga tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt
     3001 acatgcgtcc tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg
     3061 taaaacaagg gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga
     3121 aacaagtcgt tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac
     3181 ttggagaaat ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga
     3241 tggcccgcgt cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg
     3301 gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact
     3361 tagatattta atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat
     3421 gatgtcaatc taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt
     3481 gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta
     3541 tacggcatga acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc
     3601 gtcggagatg tcatgggtaa ataccatcct catggagaca ttgcgatcta tgagtcaatg
     3661 gtacggatgg cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt
     3721 ggttctgttg acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa
     3781 atcgctacag aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat
     3841 gatgatacag aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac
     3901 ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag
     3961 gtagtagcgg ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg
     4021 gaagtattac ctggaccaga ttttccaaca ggtggtttgg ttatggggaa atcaggaatc
     4081 cgaagagcgt atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact
     4141 gagatgccaa atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa
     4201 gcaaaattga tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca
     4261 gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac
     4321 gccagcgcat ccgttatctt gaataactta tataaattga catccttgca aaattcattt
     4381 ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatc
     4441 cttgaaaact atatcgaaca tcaacgcgaa gtcatcacac gacggacggt ctttgaaaaa
     4501 gaaaaagcag aagcaagagc acacatctta gaaggtttgc gtattgcttt ggatcatatc
     4561 gatgaaatca ttgcgattat ccgcgggtct aaatcagatg acgaggcaaa agcaacaatg
     4621 atcgaacggt tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc
     4681 cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg
     4741 attgaagatt taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat
     4801 gaattagcag acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt
     4861 gaggtcttaa gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg
     4921 acaaacaatg gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt
     4981 ggacgtggtg tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct
     5041 tgttctaccc atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa
     5101 ggatacgaaa ttccagaata cgggcgtaca gccaaaggaa tcccaatcat caatttacta
     5161 ggtatcgatt cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa
     5221 ggccactatc tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct
     5281 tttgctaaca tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc
     5341 gtcaatgtgc tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac
     5401 tctgtcactt tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggtcaga
     5461 gggatacgcc tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaaaatcaa
     5521 gaagtattag tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca
     5581 gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca
     5641 ctagctggat tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt
     5701 gtcattatcc gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta
     5761 cgtttgatga agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa
     5821 gctgcagaac aaacagttga agaacaagga tcagaagaat aaccatcaag actaagtgtg
     5881 taagctagca agaataaccg attaaaaaat ctggttattc ctgctggctt ttttgattag
     5941 ttgaatttct cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga
     6001 gtgtataatt agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga
     6061 acctaagagc cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat
     6121 catgtacatt attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt
     6181 cgatgcaatc ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg
     6241 ccgtttcgct tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc
     6301 ttctccatca tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga
     6361 tatcattcgt cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata
     6421 gaaaacgatg agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga
     6481 cgaaagatcc agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg
     6541 ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg
     6601 tgatttggcg caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag
     6661 gtgttactgg acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg
     6721 tgacagaagt agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc
     6781 gccaacaaag cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca
     6841 ataactctaa tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt
     6901 ctgacccatt cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa
     6961 cgaatttgat aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt
     7021 aaagtagact acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta
     7081 ctaaaaagat ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc
     7141 gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta
     7201 cctttcgtta gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc
     7261 tattcaattc ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga
     7321 aagttatggt aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa
     7381 tcatttatcc ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt
     7441 cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg
     7501 tgttcgctat tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca
     7561 gcaggcttcg actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg
     7621 agtgattgct tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa
     7681 aatcaaagat caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa
     7741 agaaaaaaag aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac
     7801 agtaaagggt gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca
     7861 gacgaacagt cagccggtga ttggtatttt atctattgat aactatgcag atgccattga
     7921 taaattagat gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga
     7981 ctggatgaat gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt
     8041 agcacagatg atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt
     8101 tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg
     8161 ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt
     8221 ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta
     8281 tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac
     8341 cgctttgcgt cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc
     8401 ggatatggat gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa
     8461 aaaagcttac atcgttatca atgagaacga gatcattcca gatgtagaac ggtgtcttga
     8521 agaaatccat aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga
     8581 gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat
     8641 ctctcagggc ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg
     8701 ggaagagttt ccaagtaaac ctctgctgac ttatattgaa tcttcagctt cttcagcttc
     8761 agagctagtt tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt
     8821 tgaggccaca ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac
     8881 aactgcacga acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt
     8941 agtacaatat ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc
     9001 tggaagcgac tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta
     9061 taatagtgtt atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc
     9121 agctttcgtc attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg
     9181 cacgatcaac gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc
     9241 agctactcaa ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat
     9301 caaagaaaat atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta
     9361 caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca
     9421 caaaattatc taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag
     9481 ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa
     9541 aagttaaaag aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg
     9601 gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa
     9661 caatacaaaa tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta
     9721 ggctacacaa acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat
     9781 gtcgtacaag aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga
     9841 aaaaagacaa accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca
     9901 aatcgatttc agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa
     9961 cgagtataat aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc
    10021 tctcattttc ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg
    10081 tggcaggatc gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt
    10141 tttcttgacg cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat
    10201 cgtcgtggcc atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg
    10261 attgacgtgg ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt
    10321 ggtttaagtt atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac
    10381 tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga
    10441 atcgttacga aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa
    10501 cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa
    10561 gtactgaact ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca
    10621 ggtctaccaa ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa
    10681 ctaatcattc ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct
    10741 caaaatgtag gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca
    10801 gaatctcttg taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt
    10861 acaggtcagc tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct
    10921 cgagcaaata tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa
    10981 tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag
    11041 ttgattgaag gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa
    11101 ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt
    11161 ggagtagaac agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca
    11221 attgaacaag atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa
    11281 ggcggagatg acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc
    11341 gagaagaacc gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat
    11401 aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca
    11461 atcccgagag ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta
    11521 atttaaacag atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa
    11581 taaacggtgt tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga
    11641 actattttga cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt
    11701 tccttaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg
    11761 agaagcagtt ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct
    11821 ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa
    11881 tatgagaagc tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca
    11941 acctctgatt cacggtgttc caaaagtagc ttcttcgaaa aataagtcga aatttcacaa
    12001 aaatttgaga agcaattttc gtacattcct tcttatttct tgaagctgac cacttctgtt
    12061 acaacctctt ttttaataac cattctaaaa agataaagtt cgttttttaa gcgtttttat
    12121 aaaataaaaa taaaaatcat tcgattttat ttgaaatatg tatctattat tgatacaatg
    12181 gttcaggatt aaataatata gaacgaggtg ttcacatgtc atctgtagta gtagtaggta
    12241 cgcaatgggg cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa aatgctgaag
    12301 tgattgcacg gtaccaaggt ggcgacaatg cagggcacac aattaagttt aacggtgtga
    12361 cttataaact gcatttgatt ccttctggga ttttttataa agacaagatc agtgtgatcg
    12421 gaaacggcgt tgtagtcaat cctaaatcat tggttaaaga attagcttat ttacatgaaa
    12481 aaggtgtaga aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc ttgccttatc
    12541 atatcaaatt ggaccaatta caagaagatg ctaagggcga aaataagatt ggtacaacga
    12601 ttaaaggaat cggacctgcc tatatggata aagcagctcg tgtaggtatc cgtattgcag
    12661 acttattaga taaagaaatt tttgaagaac gtcttcgttt gaatctagat gacaaaaacc
    12721 gtcaatttgt aaaaatgttt gattcagaac cacttgattt tgacgatatt tttgaagaat
    12781 attatgaata cggacaacaa atcaaacaat acgtcacaga tacttccgtt atcttgaatg
    12841 atgctttgga tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta atgctggata
    12901 tcgaccaggg aacttatcct tttgttactt cttctaaccc agtagccggt ggggtaacaa
    12961 tcggaagcgg tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc aaagcctata
    13021 cgtcacgtgt cggtgatgga ccatttccaa ctgaattatt tgatgaaaca ggagaacaaa
    13081 tccgcgaagt aggacgagaa tacggtacga caactggtcg tccgcgtcgt gtaggctggt
    13141 ttgattctgt agttatgcgc cattctaaac gagtttcagg tattaccaac ctatctttaa
    13201 actcgatcga tgtattaagt ggattggaaa cagtaaaaat ttgtacggct tatgaattag
    13261 atggcgaact aatctaccac tatccagcaa gcttgaaaga gttgaaccgc tgcaaaccaa
    13321 tctatgaaga attgccaggc tggtcagaag acattacagg atgcaaaaca cttgcagaac
    13381 tgccagaaaa cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt gtacgtatct
    13441 caacattttc tgtcggacct gatagaacac aaacaaacat cttagaaagc gtctgggcgc
    13501 aaatctaagg ataaaaacga cgactgaatc aggacgttct taccagagcg agtcgaagtg
    13561 tttatctata cgataataat aacttgaaga ggtagtgaaa gagacgttta gccttattgc
    13621 cgataggctg tctcttgaac actgtttatt cagaataagg gactgtgaca aaaac
//
LOCUS       DAZAFF010000076        13086 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000076 DAZAFF010000000
VERSION     DAZAFF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.077"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACG9BB_002131"
     CDS             complement(79..2199)
                     /locus_tag="ACG9BB_002131"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFN2838060.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACG9BB_002132"
     CDS             2429..3607
                     /locus_tag="ACG9BB_002132"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFN2838061.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACG9BB_002133"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACG9BB_002133"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFN2838062.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG9BB_002134"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG9BB_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFN2838063.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACG9BB_002135"
     CDS             complement(4960..6213)
                     /locus_tag="ACG9BB_002135"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN2838064.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG9BB_002136"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG9BB_002136"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFN2838065.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACG9BB_002137"
     CDS             7790..9136
                     /locus_tag="ACG9BB_002137"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFN2838066.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACG9BB_002138"
     CDS             complement(9444..9830)
                     /locus_tag="ACG9BB_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN2838067.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACG9BB_002139"
     CDS             complement(10098..10247)
                     /locus_tag="ACG9BB_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838068.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="ACG9BB_002140"
     CDS             10709..10921
                     /locus_tag="ACG9BB_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838069.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="ACG9BB_002141"
     CDS             complement(11199..12998)
                     /locus_tag="ACG9BB_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFN2838070.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2354 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaac
//
LOCUS       DAZAFF010000077        12731 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000077 DAZAFF010000000
VERSION     DAZAFF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.078"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACG9BB_002142"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACG9BB_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFN2838071.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="ACG9BB_002143"
     CDS             complement(324..893)
                     /locus_tag="ACG9BB_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFN2838072.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="ACG9BB_002144"
     CDS             1079..1981
                     /locus_tag="ACG9BB_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFN2838073.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACG9BB_002145"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACG9BB_002145"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFN2838074.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACG9BB_002146"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACG9BB_002146"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFN2838075.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACG9BB_002147"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACG9BB_002147"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFN2838076.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="ACG9BB_002148"
     CDS             8549..8734
                     /locus_tag="ACG9BB_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFN2838077.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="ACG9BB_002149"
     CDS             complement(8796..9992)
                     /locus_tag="ACG9BB_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFN2838078.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="ACG9BB_002150"
     CDS             complement(10162..11598)
                     /locus_tag="ACG9BB_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFN2838079.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="ACG9BB_002151"
     CDS             11891..12700
                     /locus_tag="ACG9BB_002151"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN2838080.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3801 a         2672 c         2249 g         4009 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt g
//
LOCUS       DAZAFF010000078        12176 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000078 DAZAFF010000000
VERSION     DAZAFF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.079"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..311)
                     /locus_tag="ACG9BB_002152"
     CDS             complement(138..311)
                     /locus_tag="ACG9BB_002152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838081.1"
                     /translation="MNKMKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGM
                     LIYFVFSDDGKYG"
     gene            597..1925
                     /locus_tag="ACG9BB_002153"
     CDS             597..1925
                     /locus_tag="ACG9BB_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFN2838082.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2127..2960
                     /locus_tag="ACG9BB_002154"
     CDS             2127..2960
                     /locus_tag="ACG9BB_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFN2838083.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2976..3713
                     /locus_tag="ACG9BB_002155"
     CDS             2976..3713
                     /locus_tag="ACG9BB_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFN2838084.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3868..4836)
                     /locus_tag="ACG9BB_002156"
     CDS             complement(3868..4836)
                     /locus_tag="ACG9BB_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFN2838085.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5068..5901
                     /locus_tag="ACG9BB_002157"
     CDS             5068..5901
                     /locus_tag="ACG9BB_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFN2838086.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5935..6774
                     /locus_tag="ACG9BB_002158"
     CDS             5935..6774
                     /locus_tag="ACG9BB_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN2838087.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6800..7360
                     /locus_tag="ACG9BB_002159"
     CDS             6800..7360
                     /locus_tag="ACG9BB_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN2838088.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7559..9280
                     /locus_tag="ACG9BB_002160"
     CDS             7559..9280
                     /locus_tag="ACG9BB_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFN2838089.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9445..10587
                     /locus_tag="ACG9BB_002161"
     CDS             9445..10587
                     /locus_tag="ACG9BB_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFN2838090.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10603..11814
                     /gene="thiI"
                     /locus_tag="ACG9BB_002162"
     CDS             10603..11814
                     /gene="thiI"
                     /locus_tag="ACG9BB_002162"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFN2838091.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4151 a         2042 c         2527 g         3456 t
ORIGIN      
        1 taaataaagc ttatgcaaag aaaaagaaat ccaatcactt gggtttcttt ttcttttatt
       61 attatgatga atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta cgaaagagta
      121 gagttggcta ctcataatca accatacttc ccatcatcgc taaatacgaa ataaatcagc
      181 attcctacac caatcaaagc acaagctgca ccaagaaaag ggatgacatt cccaaagtta
      241 tctgtattca taagtatgcc accacccaca aacaaaaagc cgccagcaat caggtttttc
      301 attttattca tctcctttct tacgttatag tatttccttc tttcaatgag tattttgaaa
      361 gctatcttat tgtatgatgt tcttctaggc ctcttcttta ttatacaggc cattttcgtt
      421 atctcataga aatattcctt tttatctgaa aactattgta taccgtatga catttttctt
      481 catgaagttg taagtatatt ctcattcaaa tgaaaatttc atcatagaag agattgtatt
      541 tctaaattct taagagtaaa atacgaagta taacttggat ttggaaggat gattggatga
      601 aagctattac gcctgatatg acaacaaaat ttaacaaaca gtttcaagca aatatcaaac
      661 agcaggcatt gcagcgggca gttgtgaaga acggaattac tgcttctgct gaaaatgttt
      721 cagcacatgt ggagaataca ccggtatttt ctatagattt ggctacgggg aaagtggcta
      781 atcaaatgca atcggggcgt tgctggatgt ttgctgcact gaacacattc cgtcataaga
      841 tgctgaatca attccattta aaagagtttg aattatcaca aaattacact tttttctggg
      901 ataaatatga aaaggcaaat tatttctatg aaaacatcat tgcaacagga aaagaggcaa
      961 tcgatagccg gaaagtagct ttcttactcg ctactcctca acaagatggc ggccaatggg
     1021 atatgatcgt ttcattattc caaaaatatg gcgttgtgcc taaatcggtg atgccggaaa
     1081 gtgcgaatag ttcaaattct cgagatttga ataactactt gaacaaatta ctgcgtaaag
     1141 atgcagtagt gttacggaaa atggttgctc aaggagaaac attagacgct attgaagaaa
     1201 agaaagaaga aatgctagaa agcatttata acttcttagc tatctcatta ggaacacctc
     1261 caaaagaatt cgattttgag taccgcgatg aagaaaagaa ctatcatctt gaccgaggac
     1321 tgacaccaca aattttttat gataaataca tcggtgtaaa attagatgat tatgttagcg
     1381 tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc ttatacagta gagatgctag
     1441 gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa actttcaaaa
     1501 aattagcgat tgctcagctt gaagaaggag aatctgtttg gtttggttgt gatgtaggtc
     1561 aatcttctac tagaactagt ggcatcatgg ccttagatgc ttattcaatg gatgatctat
     1621 ttgatacgga ttttacaatg actaaagctg aacgtttaga ttttggagaa agtctgatga
     1681 cccatgcgat ggtattgacc ggtgttgact tagtagacgg gcaatcaaca aaatggaaag
     1741 tcgaaaatag ctggggcgaa aaagttggaa agaatggctt ctttgttatg agtgatgcct
     1801 ggatggacga atatacgtac caaatcgttg taagaaaaga tttattgacg aaagagcaat
     1861 taaatgcgtt caatgaagaa ccaattgttc tatctccgtg ggatccaatg ggtgctttag
     1921 catagacgag tatttcaatg aagagtatgt agtaaagata aaaagagctg gcttaagcca
     1981 gctcttttta tctttaaagt ccattccctg ttttttatgg tccctgtgct ataattctaa
     2041 ataaaggata aggattggaa ataatccttt tatcattaaa aatatgatga cgaagtagaa
     2101 taaaattggg agtagggggg aaagtcatgg atatccaaac tgttttatct gatctgaaat
     2161 taaatggtaa agtgattcca gttgttggtg gcgatgttaa tcagacctat cgtataaaaa
     2221 ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga ttttttgaag
     2281 cagaagtaga cggattaaaa gaactgtcag cattcgtacg tgtaccggat acgtatatgc
     2341 taggtgaaac aagtgaaggc gcttatttgt taatggaatg gatcgagccg ggaaaagggg
     2401 atcaacgaga tcttgctgca gctttagcta atctccatca gcaaacagcg ccacaatttg
     2461 gttttcgtaa agataactat ttgggtacac ttgttcagaa aaattctttt gaagaagatt
     2521 ggtggacttt tttctttaaa gatcggcttg aatcccagat ttctttggca gaagaaacga
     2581 atcgatggaa tgtacagcgt caagaaaagt atctgcgatt taaagaacgt gtattgaaat
     2641 cggttgaacc aaaaaagatc actccacgcc tcttgcacgg agacctgtgg agtggtaatg
     2701 tgttctttga ccagcaaggg catccagttt ttgtcgaccc agctgtttct tatgggaatc
     2761 gggaacaaga tattgcaatg agccagttat tcggtggttt ccgaccggaa tttttagatg
     2821 cttatcaaac tatatttcca ttagaaaagg gatggaaaga ccgactgccg atttaccagt
     2881 tatattatct tttggcacat ttgaatatgt ttggagaaag ttacggttcc caagtagatc
     2941 aattactaga aaatttttaa gaggagggcg tgtttttggc aaaaaaatat accaaaaagc
     3001 atgaagtttc atattatgag tgtgacatca accaaacgat gacatttcct tcgatgttag
     3061 gagtagttat caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc agtgaattcg
     3121 tcaatagctt tggacttact tgggtcatca cgaattattc catggagatt aatcgtctcc
     3181 ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat ggagtacaat aagtttttct
     3241 gttatcggaa cttctggatc catgatgaag atgggaaaga attagtcaaa atagaatctg
     3301 ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa gagatcattg
     3361 ctccttttga gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt gaaaagattg
     3421 attcgggctt catgttgcca tatcgtgttc gtttttatga tatcgatagt aatcagcatg
     3481 ttaataatgc gatgtatttt aattggatca ttgacgtatt aggatatgac tttttgacta
     3541 cgcatgttcc agaaaaagta attattcgat ttgataaaga agtagagtac gggaatgaga
     3601 tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa catgaaatcc
     3661 gaatgcaaga acagctgtat tgtgaagcaa atattatatg gaaaaataaa taaaaaagag
     3721 gttgtgacag aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa ttgtttctca
     3781 aatttttgta aattttcgac ttagttttcg aagaagctat ttttgtcaca gcctctcttt
     3841 ttaagtttag ctattagata atgatgttta gttttggata aacgaaatca attcttcacc
     3901 cgacaaacca tagtttacag caatcaatag ttttaaacgt gctttttgac tattaatact
     3961 gttacagaaa ataatcccca ttttttctag ttcttttccc ccgccttcat aatcataaac
     4021 aggctctgcg attccattga aacaacgaga aacaagtact acagggattt tttggacaag
     4081 aagcttttcc aaagcagcta aggcttgtgg agggagattc cccgcaccta aagcttcgat
     4141 taccaaaccg tctactttcg tatgggcgat ggcttccaat aagtcccctt gcatcccagc
     4201 atatgcttta acgatcggga tagtcccatc tacagcggaa atgtccagtc gctttgtttc
     4261 taaaagttcc tgcaagaata aaattcgatt cttagttact aagccgatgg gacccaatgt
     4321 aggtgttcgg aaagtagcaa catttgtggt atgggtcttt gtcacgtagc gtgcagaatg
     4381 gatctcatcg ttcatgacca ccaaaacccc cttatctagc gcttcttcac aacttgcgac
     4441 acgaatcgca ctttcaaaat tataaaggcc gtcacttcca agttcattac ttgaacgcat
     4501 tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa aaaaagcagt
     4561 ttcttctaat gtatctgtac cgtgtgtgat caccactccg gatatacctg atgcataagc
     4621 ggacaagata cgttctttta ataccaacat gtgagaaggc atgatgtgtg gtgagggaag
     4681 attgaagata tcttcaacta ataattctac atgcttgggg attgtgattt ctgcatctaa
     4741 taatgggttt gccacatcag gactaacacc gcccgtgagc atgttttctt tcatggcgat
     4801 cgttccgccg gtgtgtagta caagtatttt tttcatttta cagacttcct tacgaataat
     4861 tttgcatagc cgttaaatct tattgtatac tatataaaag agttttgcta tcagtgaaga
     4921 ataacgaata gcctaactta tcataaaaaa gctgacctta ttaaaatgga aaagactcaa
     4981 ccttgacctc ctaatacaat ggagtatctg gttgagtttc ttttacaaga aagcagccaa
     5041 ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg gattaacatc gtttaaagaa
     5101 catgacaaat taactggtaa aaagaataac actctttatg aatatgcagg gtttctaccc
     5161 ttagtggaat tagatactgc ctattatgga attccccgaa agtcctcagt tgagaattgg
     5221 ataagtgaag tacctgaatc atttcgcttt gtagtcaaag tctatcaagg aatcagcggt
     5281 caaggagact ggaagcctta ttatcagacc gaagaagaga tgattgcgca ttttttagat
     5341 gttatgtcgc caatggtaga tgctggaaag atgttttgct ttttagtcca attcccctca
     5401 tcattcaaat gtacgaaaga aaatgtccag tacttgcgca aacttagaaa gtggtttgag
     5461 ggttatcctg tttctataga attgcgggat tattcttggt acagcgatga atttcttgaa
     5521 cgtacaagaa actttatgtc tagagccgct ttttctttag ccatcatcga tgagccacag
     5581 ttgctgaata caacgattcc ttttgatcca tttgtcacca atccgaactt tgctcttttt
     5641 cgattccatg gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg gcgtaaaaaa
     5701 agaacgttat atcgttattc agaaaaagaa ctgcaagaac tagcacatga agtcaaagta
     5761 gttgattcta agacaaaaga ggtaggtgtg atttttaata ataattctgg tggtgatgcg
     5821 gctgaaaatg cattagcact gcaaaaaata ttaaatatca agccagatgg actcaatcca
     5881 acccaaatgg atctgtttta agggcttatt ttattcaaga aaggggcgaa gtctatgatc
     5941 aaagctattt tttttgatat tgacggaaca ttggtcacca atcgttcaaa agcgttggaa
     6001 tcaacaaaac aagcaattga atatgctcgg aaaaacggca ttttatgcgg tgtagcaacg
     6061 gggcgcagcc cagtaaagat caaagaaatc attgatgaat tggaattaga tatgtatgtc
     6121 gtgtataatg gtcaactggt ttttactgca gatcgcacga ttatcgacca cccttttgaa
     6181 caaaaagtat tggaacatat tgttgaattt gcagatgaga accatcgaca aatcgttttt
     6241 ggtgcgagaa atcggttaga tggatcaacg acgatgctat taggacaatc gatttttatc
     6301 aagcgactag tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa aaaaatcctc
     6361 caggttttta gccctcaccg acaaaaggat cgttatgaaa cgttagagat attaaaagaa
     6421 ccaatttatc aatgtatatt gctcagtccg gagtcagaac agaaaaagtt agaacatcgc
     6481 tttcccgagt gtacgtttca gcgttcaaat acgtatactg tagatatcat cccaaaaggt
     6541 ggttcaaaat tactaggcat ccaagctttc gcaaatgctg tcgaaattga aatggaagaa
     6601 attatggcat tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt aggaattgga
     6661 gtggccatgg ggaatgctca gatcgaagta aaacatgctg cagactttgt gactcaatca
     6721 aatgaagaag atgggatata cgaaggactg aaacacttcg gtctgattta ttagtcaatg
     6781 aaagaaggaa aaaatcagta tggaagcaga gacacctttt caaaaagtag aaacattcca
     6841 caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa atgaagctca
     6901 tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta cttcacctaa
     6961 taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata aagcagaaga
     7021 aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag tagatgcact
     7081 gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag tgcctgatcg
     7141 aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg gaaaacctca
     7201 ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg aatatgctcc
     7261 agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg ctaagaagaa
     7321 atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc actatttttg
     7381 ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat gcatgaaaat
     7441 agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt tggatttatg
     7501 ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg aaccataaat
     7561 gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca ttgtcgtctt
     7621 tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct tagaaaaacg
     7681 caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta aaaaaatgca
     7741 tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga atgacatatc
     7801 tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc tcaatgaaag
     7861 ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa tggaccaaat
     7921 ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa cggaagaacg
     7981 caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag aaaaagtcag
     8041 agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga tcaaagtgat
     8101 cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg ttgaagcccg
     8161 cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga tgaaaaaaat
     8221 tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag aaatcagtga
     8281 tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc ttgctgaaga
     8341 tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa aaacagaagt
     8401 tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt tatatgatat
     8461 cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa cgattgcaga
     8521 gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg atcacacatc
     8581 tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc aaaccgaagt
     8641 ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac acgaaattcc
     8701 ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag atatcgaaaa
     8761 gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa aagaagcgca
     8821 agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg tagaaaaaca
     8881 acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta ctgaccgtat
     8941 tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg tcaatcgttt
     9001 agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg atttagtaga
     9061 tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc atacacacga
     9121 agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat atcactatca
     9181 agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg ctttcaaacg
     9241 catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag ttagtaaacc
     9301 agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt tgaaaattga
     9361 aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat aatagaaaag
     9421 aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac aaccccaatc
     9481 tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg gaatccatca
     9541 agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag aaagcagatc
     9601 gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg aacagaagga
     9661 gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa tcatatgatc
     9721 atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa agaattagga
     9781 atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga cgaattgaaa
     9841 gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa tgaagtagga
     9901 tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa aattcatttc
     9961 catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt gacagaaaga
    10021 gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac tggtttcatt
    10081 tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca agaaagaaat
    10141 cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc tttacgttta
    10201 tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt agctctaatc
    10261 aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga ctatgcaccg
    10321 catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca tgccttagaa
    10381 gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca tatggcaagc
    10441 agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt tcgaatcagc
    10501 ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt caagcagctt
    10561 tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata cagtgaaatt
    10621 atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt tatcatgcaa
    10681 ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat ccatgctgat
    10741 cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat tccaaaacta
    10801 gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa agatgtcgaa
    10861 gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa agaaacattt
    10921 aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga attgaatcaa
    10981 acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat gcgtcaacca
    11041 gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta tgaaacgatc
    11101 aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat gctttctggg
    11161 gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga aattgaagct
    11221 gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc aaaagatcta
    11281 acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc atttaccgag
    11341 attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat tactcgtcga
    11401 atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt cattttgaat
    11461 ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc tatcaatgaa
    11521 gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga aatcattgaa
    11581 gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga ttgctgtacg
    11641 atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca tcaatatgaa
    11701 gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa gatggaagag
    11761 atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct ataagaggtt
    11821 atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata gctccatgta
    11881 ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt atcaagaagt
    11941 tatggaaaag ccggttcttt tttactaact gaaaaataaa attttattta gagatcaagc
    12001 tacagttgat aaatgatata aaaaagagaa tttcccatga aatagcgagg ttgtgacaga
    12061 agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa atttttgtga
    12121 aatttcggct tatttttcga agaagttact tctggaacac cgtgtatcag aggttg
//
LOCUS       DAZAFF010000079        12135 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000079 DAZAFF010000000
VERSION     DAZAFF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.080"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACG9BB_002163"
     CDS             <1..610
                     /locus_tag="ACG9BB_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN2838092.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACG9BB_002164"
     CDS             723..1001
                     /locus_tag="ACG9BB_002164"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2838093.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACG9BB_002165"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACG9BB_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFN2838094.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACG9BB_002166"
     CDS             1458..2213
                     /locus_tag="ACG9BB_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN2838095.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACG9BB_002167"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACG9BB_002167"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFN2838096.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACG9BB_002168"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACG9BB_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFN2838097.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACG9BB_002169"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACG9BB_002169"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFN2838098.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACG9BB_002170"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACG9BB_002170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFN2838099.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACG9BB_002171"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACG9BB_002171"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFN2838100.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACG9BB_002172"
     CDS             6950..7492
                     /locus_tag="ACG9BB_002172"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFN2838101.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACG9BB_002173"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACG9BB_002173"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFN2838102.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACG9BB_002174"
     CDS             9085..9987
                     /locus_tag="ACG9BB_002174"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFN2838103.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACG9BB_002175"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACG9BB_002175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFN2838104.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACG9BB_002176"
     CDS             11430..11852
                     /locus_tag="ACG9BB_002176"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFN2838105.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2155 c         2677 g         3268 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttatt
//
LOCUS       DAZAFF010000080        12101 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000080 DAZAFF010000000
VERSION     DAZAFF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.081"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..604)
                     /gene="frr"
                     /locus_tag="ACG9BB_002177"
     CDS             complement(47..604)
                     /gene="frr"
                     /locus_tag="ACG9BB_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFN2838106.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(607..1329)
                     /gene="pyrH"
                     /locus_tag="ACG9BB_002178"
     CDS             complement(607..1329)
                     /gene="pyrH"
                     /locus_tag="ACG9BB_002178"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFN2838107.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1465..2346)
                     /gene="tsf"
                     /locus_tag="ACG9BB_002179"
     CDS             complement(1465..2346)
                     /gene="tsf"
                     /locus_tag="ACG9BB_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFN2838108.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2445..3227)
                     /gene="rpsB"
                     /locus_tag="ACG9BB_002180"
     CDS             complement(2445..3227)
                     /gene="rpsB"
                     /locus_tag="ACG9BB_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFN2838109.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3586..4065
                     /locus_tag="ACG9BB_002181"
     CDS             3586..4065
                     /locus_tag="ACG9BB_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFN2838110.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4282..5373
                     /gene="phnW"
                     /locus_tag="ACG9BB_002182"
                     /pseudo
     CDS             4282..5373
                     /gene="phnW"
                     /locus_tag="ACG9BB_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            5366..6151
                     /gene="phnX"
                     /locus_tag="ACG9BB_002183"
     CDS             5366..6151
                     /gene="phnX"
                     /locus_tag="ACG9BB_002183"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFN2838111.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6497..8188)
                     /gene="argS"
                     /locus_tag="ACG9BB_002184"
     CDS             complement(6497..8188)
                     /gene="argS"
                     /locus_tag="ACG9BB_002184"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFN2838112.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8610..9557)
                     /gene="arcC"
                     /locus_tag="ACG9BB_002185"
     CDS             complement(8610..9557)
                     /gene="arcC"
                     /locus_tag="ACG9BB_002185"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFN2838113.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9672..10691)
                     /gene="argF"
                     /locus_tag="ACG9BB_002186"
     CDS             complement(9672..10691)
                     /gene="argF"
                     /locus_tag="ACG9BB_002186"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFN2838114.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10782..12011)
                     /gene="arcA"
                     /locus_tag="ACG9BB_002187"
     CDS             complement(10782..12011)
                     /gene="arcA"
                     /locus_tag="ACG9BB_002187"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFN2838115.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3309 a         2527 c         2185 g         4080 t
ORIGIN      
        1 aaacagagga agcggaatga tccgcccctc tgtttttatg attttattaa acttccaata
       61 attctttttc tttatcagca gcgatcttat ctacttcttt aatgctgtta tctgtcaatg
      121 tttgtacgtc tttttctaat ccgcgcaaat catcttcggt aatatcacca tttttttgtt
      181 gttttttata ttcatcgatc gcatcacgac ggatgttacg aatcgcgatt tttgcatttt
      241 ctgcttcttt tttcacatct ttagctaatt cttttctgcg ttcttctgtc agttgcggga
      301 taactaagcg aatgacattt ccatcattgg ttggactgat tccgatatca cttgccataa
      361 tagctttttc aacatcttga agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg
      421 cttctggaat ttggattgat gccatttggt tcaatggtgt tggcgcaccg tagtagttca
      481 cagtaatacg gtccaaaagg ctagcgttcg cacggcctgc gcggatttgc ccaagttcac
      541 gttgaagatt ttgtgctgct tttttcattt tttcttttgc ttctgtcata attgcatctg
      601 ccatttttat ttccccctta cagttgttcc gatattttcg cctaagattg cacggcgaat
      661 atttccgtgt tcatttaaat tgaagacaag cagtggaata tcattatcca tgcttagtga
      721 acttgcagtt gaatccatga cttgtaatcc tttggcaata acttccagat gagtcagttc
      781 ttcaaatttt actgcatttg catctacttt cggatcagca gaataaacac cgtctacatt
      841 gtttttagcc atcaggataa catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt
      901 gtctgtcgag aagtacgggt tgccggtacc gcctgcaaag ataacgatcc gtcctttttc
      961 taaatgacgt tctgctttac ggcgaatata tggctctgcg atttgacgca tttcaatcga
     1021 agtttgcacg cgtgtcggta cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt
     1081 cataactgta gctaacatac ccatgtaatc agcttgagca cgttccatgc ccatttgtgc
     1141 tccgatttgt ccgcgccaga tatttccgcc acccacaacg attgccattt caacgcccag
     1201 ctcgtgaact tctttgattt cttggatgat ttctttgatt actggtggtt tgattccaaa
     1261 tcctgcatcc ccagctaaag cttctccact caattttaag actacacgct gatatttcgg
     1321 tgtgaccatg ttcattcctc caatattttc tattgccatt ttaccatact tagatgattt
     1381 cgacactcta agactggttt ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc
     1441 gttcccgtct gttattcaac agaattattt tttcacttgg ctcataactt cttctacaaa
     1501 gttatcttcg cgtttttcga taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt
     1561 tgcgcctttt gatgcaacgt atttttcaac agtcatatca ggatctttaa cgaatggttg
     1621 gtccaccaaa gcgatttctg ctttgaattt cttcaagcgt ccttctacca ttttttcaac
     1681 gatattcgct ggtttgcctt cgttcaatgc ttgttctgta agaacagttt tttcatgttc
     1741 taattcagct tctggaattt gtgattcgtt tacataacga ggattgatag ctgctacgtg
     1801 catagctacg tctcttgcaa ctgtttcgtc agttgttcca tctagaacag tcaatacggc
     1861 aatacgtccg cccatatgca agtatccacc aaatgcagca ttgtcgtctt tttctacaac
     1921 ttcaaagcga cggaagctga ttttttctcc gataacttga gttgcttcga tcaagtcata
     1981 ttcaattgta cctttatctg ttttgatttt cattgcagct tccatgtctg ctggtttgtt
     2041 ttcagcaaca agttctgcaa tttcttttac taaatcttgg aacatttcgt ttttagaaac
     2101 aaagtctgtt tctgagttta cttcaacgat tgctgcagtg ttacctttca cagcaactga
     2161 agccaatcct tcagctgcaa tgcggtcatt tttcttcgca gcctttgcca ttcctttttc
     2221 acgtaaaaga tcaactgctt tttccatgtc gccttctact tcaactaatg cttttttcgc
     2281 atccatcatt cctacgccag tcatttcgcg tagttctttt accattttag ctgtaacgtc
     2341 tgccatgttt aactcctcct gtgtgttttt ctttttaaaa aaagctgctt caaaggagag
     2401 tgaggcttgt cgcccagcct ttgaaacagc tccatcattc aaaattattc tgctgaagaa
     2461 ttgtcgcctt caacaacatc tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg
     2521 aaatcttctt caactacttg atcttcacct tggtttcctt cgatgaaagc atcagccatt
     2581 tttgaagtaa tcaatttaac ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg
     2641 atttcatctg gatcgcagtt tgtatcaacc atagctacga ttgggatatt taatttgtgt
     2701 gcttcttgaa cagcaatacg ttctttacgt gggtcaacga tgtacattac atctgggatt
     2761 cttggcatat cagcgatacc gcctaagaat ttttcaagac gttcacgttc tttgttcaaa
     2821 ccagcaactt cttttttagg tagaacttca aaagttccat cttcttccat tgcattgatt
     2881 tgtttcaaac ggctgatacg tttttggata gtatcccagt ttgtcaatgt tccgcctaac
     2941 caacgatggt taacgaagta ttgtcctgca cgagttgctt cttctttgat tgcttcttgt
     3001 gcttgttttt ttgtacctac gaataatgct acgccgcctt cttcagcaac gctcttcatg
     3061 taatcataag ctgcatctac taatttaact gttttttgca agtcaatgat gtagattccg
     3121 tttctttctg tgaagatata tttcttcatt tttggattcc agcgacgagt ttggtgacca
     3181 aagtgtacgc cggcttctag taattgtttc atagaaatta ctgccatgtt tgtttcctcc
     3241 gatttggttt tatttttcct cttcttatct tctaccttgc cggcaaactt tttcaagcac
     3301 ctaccgacaa tccaataaga atgtggattt gctgatgcat aaagcaccct cagctattat
     3361 acagacaaaa gatccgtatc gcaagagcaa atatactttt ctgctcaaca ttcaatagtt
     3421 tacctgaaag ataaaaagaa ttcaagaaaa acagtagatt tctctttgtt tctttattat
     3481 cctttttcca taaaataatt ttaggtataa aagacgaata ttattgggca atccttcttt
     3541 tttttgttat gatacatgtg aaatctttcg taaaggagaa agaaaatgcg caaagctcaa
     3601 agacatttgc tgattaaaca agtcattgaa gaattcccga tccgtacgca agaagaacta
     3661 ttaaacaaac ttgggcatta tggcgttacc gccacgcagg caacgatttc tcgagatatc
     3721 cgtgatctaa aaatcgtgaa ggcacctgat gaaaatggcg tttcccgctt cgtattgttt
     3781 cgcgaaaaag gaactcctga gactaggact gaggaagagg atcggctggt tcaaatgatt
     3841 gaagacattg tattgaaagt cgaacgagtc cactttttga cgatcgtcaa taccttgcca
     3901 gacaatgctc atttatttgc atctgtttta gatgagatca aacctccctc tattgtcagt
     3961 acaattgctg gattcgatac gatcatggtc atctcggaaa atgaggagga cgcaaaacga
     4021 gtagaagaat ttcttcacca tccaactgaa gtatcacttt tttaatcaga gcacgtattc
     4081 ctaatatgga atgcgtgttt ttttattttc aatattttcc ttattaaata aacctttaca
     4141 ctctttacaa aaatacatca ttttttattc aattttttca acaccttgat tgaatcacaa
     4201 taatcatcat ttatattgga tgttaacaag agcagcttct tgctctttgt gtatttcaaa
     4261 aaagaaatga aaggaaatca tatgtataaa ttattaactc ctggaccatt gacgacttct
     4321 gaaacagtga aaaatgctat gctgatcgat cattgtactt gggatcaaga atacaaagaa
     4381 attacacaat ggataagaaa aaaattattg gatcttgctc aagtatcgga aggaatatac
     4441 acaacggtct taatgcaagg tagcggctcc tttggggtag aatcggtatt aagttctgtg
     4501 atcaggccag aagatactgt actgatttgt tcaaacggcg catatggaca acgaatgatc
     4561 aacatgtgcg aatgtctgaa actgaactat accgtttatg aggtatcgta acatcaaatc
     4621 ctccaagctc atgtgattac acagctgatt gaagagaatc cctctatctc cgcactagca
     4681 atggtccata gcgagactac ttccggtatt ttgaataatt tggaagagat tgctgctgtc
     4741 acacaagaaa aagatctttt gtttatagtg gacgcgatgt caagctttgg tgggataccg
     4801 attcgtgtag ccgaactagg gattgatttt ttgatttcca gctcaaacaa atgtattcaa
     4861 ggtgttccag gcttttcttt tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat
     4921 gtgagtcgaa gtttgtcact tgatctatat gaccagtacc gcgtgatgga aaacgagaat
     4981 ggcaaatggc gtttcacttc ccctacccat acggttttgg cttttcaaca agcgctgaag
     5041 gaactagagg cagaaggagg aatcgaaact cgatattcac gttatctcgc caataatcat
     5101 gtactacgac aaaaaatgac agagatgggg ttccagcctt tcattcaaga atatcagggg
     5161 ccattcatca cctcttttct ttaccctaag gactggcgat ttgattttaa tcatttttat
     5221 cagtatttaa agcaaaacgg atttgtgatc tatcccggga aaatcagcga tcatgaatgt
     5281 tttcgtatcg gaaatattgg cgagatctat ccggaagata ttttggaact gacagcaatt
     5341 atcaaagact ataaggagaa cgttaatgat taaaactgta atttttgatt gggcaggaac
     5401 aactgtggac tttggctgta tggctcctgt ccacgctttt agaaatgctt ttctagaaaa
     5461 aggaatccaa ctgactgaca aagaaatccg cgaaccgatg ggaaaattaa aatgggatca
     5521 tatccaacag cttctttccc tcccttctgt caaaaaacaa tggatagcta tttatggaca
     5581 actacctcag gaaaaagagg tggaggaatt gtatcaacaa tttgaacatt atctgtttaa
     5641 agatttggtc aagcattcta ccttgaaacc cgacacattg gaaacgatcg aacggttgag
     5701 aaacgctggg atcaatattg gttccacgac tggatttact gctcctatga tgacaatcgt
     5761 agcagaaacc gcaaaaaaag aagggtattc accagacttc atggcaactt ctgaagacgt
     5821 cgaagggtat ggtcgaccgt ttccttatat gatccataaa aacatgcagt acttcaaaaa
     5881 caagtcggtt gatgaagtaa tcaaagtcgg agatacggtc tcagatatcc aggaagggaa
     5941 aaatgccgga gtgttgacag taggtgtaat tgaaggtagt tcgctaatgg ggatttctta
     6001 tgatgagtac caaaaattag agatggggaa aaaagatcgt ttaaaacagt ctgttgctct
     6061 tagattccaa caagctggcg ctgatatttg tgtaaacaat ttaaaagagt taacagaata
     6121 cattttgtac gctgacaaga atccagaatg acttttatac aaaatcagag gtcgtgaaaa
     6181 agctgtactg catcaagtaa taagaacagc caaacaaaaa tagctcctca tatttttcgt
     6241 ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag
     6301 ggagctgtga cacgactttt gtcacagtcc ccttatttac aaacaaacag tgtttaagag
     6361 acaacctctc cactaaacgg tgttcattca gctgactctc ggtatttgct caaaaaacga
     6421 taaaatttga aaagctattt tctcttttgt ttctctaata ctcggatcag aacgctgatt
     6481 tcacattctc tttttcttac atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt
     6541 ctttcaataa tacagtcact gcataaacta gagctaagcg tgcttctttt tgttcatcat
     6601 cagctaaaat cttcgtatga gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat
     6661 gtttcgcaat cactgacggt tcgtattttt ccccagcaga caagaccgtt tcagggtatt
     6721 tttgaactaa tttaaccact tcccagctgg tatcgtcatt caatgcatag tctgctgttt
     6781 ctgaagggac aaatcctgct ttttccaaca ggctgactgc acgagcatgt gtatattgca
     6841 cataaggtcc agtttctcct tcaaatcgaa caacttcttc caggttaaag tcaaatgtat
     6901 tcagtcgatc gtttttcaaa tcgtggaaaa tgactgcccc aaccccaact tgtttagcta
     6961 cagcatcttt gttttccaaa tcagggtttt tctcgctgat ttgttcttta gcagattgga
     7021 ttgcttcatt taatacttct tctaataaga cgatctttcc tttacgagtt gataattttt
     7081 taccaccttg cgtgatcaaa ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc
     7141 ccatttcctt caagacagct ttcaactgtt taaaatgata gctttgttca ttccctacta
     7201 catatagaga ttgtttgaag tcgtaagtac gtttacggta caatgcagca gctaagtcac
     7261 gtgtaatgta tagtgtcgca ccgtctgact tcttaatcaa cgctggattc aggtcataag
     7321 cagataggtc aacgatctct gcacctttat cctcgttcag caaatgtttt tcttctaaaa
     7381 gtttgacgat ttcatccatc ttgtcattgt agaaagcttc accgttcaac gaatcgaaac
     7441 gtacttccag cagatcatag attttattga attctttcat cgattcatca cggaaccatt
     7501 gccataattg aatagcctct tcatcgcctt cttccagacg tttgaaccaa gcacgagctt
     7561 cttcatttaa ttctggttct gtttcagcta cttcatggaa ttggacatat aaacgaagca
     7621 attcattgat tggttctgct ttaacagctt cttctgtacc ccattttttg taagcgacga
     7681 tcagttttcc aaactgtgtt ccccagtcac ctaaatggtt gatgcggatt ggctgataac
     7741 cgattttttc cataatgaaa ccgatcgaat taccgataac tgtcgaacgc aaatgtccca
     7801 ttgagattgg tttggcaata ttaggagaag acatgtcgat tggtactgtt ccttgattcc
     7861 cgatattgct atccccataa tgttcttttt ctttcactac tgtttgcaaa acttttttgc
     7921 tgataagttc tttattcatg aaaaagttca agtaaggtcc gacaacttcg attttttcaa
     7981 agttggcaga atcgattttt tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt
     8041 aaacttttgc taatgaaaag gctggaaatg caacatctcc gtgttcagct gattttggat
     8101 tttctagtaa ttgttcgact tgttccagtg ttaaatcatc tttgacagcg tcatagacag
     8161 cttttgcgac aagatcttta ttattcatct ttattcctcc taaagtgctc ccttgattgt
     8221 taaacaaaaa aaatctctag cgacggcata cttagtatgc catcactaga gacgagattt
     8281 cccgcggtac cactccaatt gtcctaaaaa aggaccagct ccatctgata tcggtagacg
     8341 accgtctgtt taaaacagaa ttctccaagt gcgcttcgtt gtatcttctt cattcggctt
     8401 ccaccacttc cgaattcgct agccaagaat gatacaagta ctctcttgtt caacaaatct
     8461 tagtcttatt aaactttctt gaattatatc agaaaaaaaa acgaaaggct agtcgtttct
     8521 gataaaaaaa caatccagat ggcctaaagg agaagaaata ccacctggat agctataaat
     8581 catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt tccttcttca
     8641 gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc gtttggtttt
     8701 gcttcaacaa atgcaatagc tgcctctact ttcggaagca tgcttcctgg agcaaattgc
     8761 ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt cttttgatct
     8821 ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag caaatccgca
     8881 tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc ttctcgtcct
     8941 tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga gactgtgata
     9001 actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat gggtttaggg
     9061 gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac agtggagtca
     9121 gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg tttacttgga
     9181 tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga aaccacatct
     9241 ttatcgatcc ctttttcctt cagtacttcg cccatggcat tttgtaacca gtaaccgata
     9301 gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt ctcagaatca
     9361 gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg ggagatgatc
     9421 aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat taacgcttct
     9481 tgttgggctt ttgcacttgc atccgtagac aagattgcat taccccctaa agcgacaact
     9541 acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt tctagataac
     9601 aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc gtgagatgaa
     9661 tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc catgatcgct
     9721 ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt gctgcggaag
     9781 acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa
     9841 acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg atctgtgttg
     9901 tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt gatacgttct
     9961 tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac gtctgctccg
    10021 tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt ttcagcaaag
    10081 ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc aacgatacgc
    10141 atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt gtttcgacca
    10201 tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg gatagttaaa
    10261 aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca aactggaaca
    10321 ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa ttcgattcca
    10381 tcaaacatac gacctagtac tttagctgta tcttctgtgg attctttttt acctaattgg
    10441 atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc agtagtaaat
    10501 gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt tccttctaga
    10561 tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa
    10621 tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc ttggaatact
    10681 gattctttca tattaaacat tctcctttag attgttttta tttaagatta aagaggaaat
    10741 cccgacggca gctgacagta agaccgccgt caggaaacaa attacttcaa gtcttcacgg
    10801 acaagtggtt ggctcatgca gcgtggacca ccacggcctc gtgacaattc acttgaattg
    10861 atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata atttcggtta
    10921 taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt ccattgttct
    10981 cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc atctaatcca
    11041 agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat atcatcacca
    11101 tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc tggatggata
    11161 gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat tgcacggttg
    11221 tttggaattt tgattgctaa cactttttca aatcctgaat tttttgcgaa taggtggcgt
    11281 gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc tactacttta
    11341 tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg atccaaccat
    11401 acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc tgtaaacatc
    11461 gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt tcccattgat
    11521 gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa tggatagtca
    11581 tcgtctgctg acagatcgta tagactgcta gaacgtactt caatatcttt tgtacgaacc
    11641 ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa ctcatgaaga
    11701 ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt caagaattgt
    11761 tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta aagagtctcg
    11821 acgccttcat tttgcaatgt ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga
    11881 tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt ttccacttct
    11941 tgtccaggcc ttttcagtaa aactgtcttt aatttcccta tttcagagaa aacgtgaata
    12001 ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat atcctccttt
    12061 tgtaatcact ttcataattt tgtctcattc aaaatgagac a
//
LOCUS       DAZAFF010000081        11738 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000081 DAZAFF010000000
VERSION     DAZAFF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.082"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..160
                     /locus_tag="ACG9BB_002188"
     CDS             <1..160
                     /locus_tag="ACG9BB_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2838116.1"
                     /translation="LAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMPASEAAK
                     KDLIDILR"
     gene            561..1166
                     /gene="fic"
                     /locus_tag="ACG9BB_002189"
     CDS             561..1166
                     /gene="fic"
                     /locus_tag="ACG9BB_002189"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFN2838117.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1182..1754
                     /locus_tag="ACG9BB_002190"
     CDS             1182..1754
                     /locus_tag="ACG9BB_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2838118.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2006..2263)
                     /locus_tag="ACG9BB_002191"
     CDS             complement(2006..2263)
                     /locus_tag="ACG9BB_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838119.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2389..3204)
                     /locus_tag="ACG9BB_002192"
     CDS             complement(2389..3204)
                     /locus_tag="ACG9BB_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2838120.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3721..5046
                     /locus_tag="ACG9BB_002193"
     CDS             3721..5046
                     /locus_tag="ACG9BB_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN2838121.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5039..5389
                     /locus_tag="ACG9BB_002194"
     CDS             5039..5389
                     /locus_tag="ACG9BB_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838122.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5371..5556
                     /locus_tag="ACG9BB_002195"
     CDS             5371..5556
                     /locus_tag="ACG9BB_002195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838123.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6460..6735)
                     /gene="prgO"
                     /locus_tag="ACG9BB_002196"
     CDS             complement(6460..6735)
                     /gene="prgO"
                     /locus_tag="ACG9BB_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFN2838124.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6707..7660)
                     /locus_tag="ACG9BB_002197"
     CDS             complement(6707..7660)
                     /locus_tag="ACG9BB_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2838125.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8272..9765
                     /locus_tag="ACG9BB_002198"
     CDS             8272..9765
                     /locus_tag="ACG9BB_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFN2838126.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9900..10196
                     /locus_tag="ACG9BB_002199"
     CDS             9900..10196
                     /locus_tag="ACG9BB_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFN2838127.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10297..11148
                     /locus_tag="ACG9BB_002200"
     CDS             10297..11148
                     /locus_tag="ACG9BB_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838128.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11169..11381
                     /locus_tag="ACG9BB_002201"
     CDS             11169..11381
                     /locus_tag="ACG9BB_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838129.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4036 a         1810 c         2175 g         3717 t
ORIGIN      
        1 tcttgcttat agtgttggtt ctttgataaa ggtaaacgtg tcacttgatc tatttactat
       61 tttactagcg gttggggtat cttcggttat tggactggtc ttttctgtaa tgcccgcttc
      121 agaggctgca aaaaaagatt taattgatat tcttagataa aatcagaatg acaagaatat
      181 ttacgttcta gattctaaaa taaaacacaa agagaccaac caggcaccct attgtttctt
      241 tgtagtatag agatattctt tggcttgtaa gagatcaata gagttaggtg accggagttc
      301 gaaataaagg aaatttttga ttgtatagga gaattcttta tccctaatat ttagaatttt
      361 gagtatgcca tgttgatgtt ttagattctc tataaatata gaggtgtatc aaaggtcaac
      421 tagactgtgc aaaatagaga cacttgaaaa atctatcggg ggtcagaaac ttaatagttt
      481 ttgacacata aacaaaatcg cgatataatg gggttgagag tgtttcataa acttatgtca
      541 taaagttaag gagatttcgc atgattttag agaataaatt gggattaacc aatcaagttg
      601 agttggctaa ggttgaagaa aaactcagta aacaaaaagc gaaagaattg tacgattgtg
      661 gaaaaataaa tgaaatcgaa gttggtacat ttaaaggttt atctgaaatt catgaatttc
      721 tattttcgga tatttacgat tttgccggaa aaatccgatc ggtcaatatt gcaaaaggaa
      781 attttcgatt tgcaccagtt atgtatttag aacattcgtt acagcatatt gatcagatgc
      841 cacaaacttc ttttgaagag attatcaaga aatatgtgga gatgaatatc gctcacccat
      901 ttcgagaagg aaatggccga agtactagaa tttggctgga tctcatttta aaggaagaat
      961 tgcaaaaagt tgtagattgg aacttaatcg ataaagcaga ctatctttct gcaatggagc
     1021 gaagtcccat aaacgatttg gaaatacgct atctaatttc caatgctctg actgataaaa
     1081 ttgacgatcg cgagttatat atgaaaggaa tcgatgtcag ctatttctat gaagggtatt
     1141 ctgagtatac gatagatgat ttgtaaaagt aggtggacca aatgacgaaa tatggatatg
     1201 cacgtgtctc aaccgcttct caaagtacaa aagaacaaat tcaacagttg cttgagaacg
     1261 gcgtagagaa gaagaatatt ttttcagaaa aatttacggg tacaaaacgg gatcggccaa
     1321 aattcaatca gctgttggct ggtctgaagc ctggagatga aatagttgtt acgaagcttg
     1381 atcgatttgc tcgtaacacg agagaagcat tagagatcat tgaaccttta ctcgatgata
     1441 acgtaacgat caaggttttg aatctaggaa cgattgaaaa tacgtccatg gggcgaatgg
     1501 tgacacggac gttgttgagt gtagcagaaa tggaacgtga tatgattgtg gaacgtaccc
     1561 aagaaggaaa aatgtttgca agaaagaata atcctaattt cagagaagga cgtccgaaag
     1621 caacaatcac accgaagaag aggcacgcat atgaactgct tacttcaggt aaatcgtata
     1681 aggaagttga agctataacc ggtttcagtc gcagtacgct ttttagaata aagaagaaaa
     1741 ttgaagaaag ttaaaaaaga agtctcagcg aaccagacag gtaaatactt aatgcgttct
     1801 agatatgagt ctattagcat gttgtgaaat ataatatttt tttatttact tgatttctgc
     1861 tcaaaaaatg tgtatggtaa ctgtaaaagg taaagtaatt aaaggtatgc tagccacgct
     1921 tactatatta ggataccatc atatatttat aatgaacgat ttgttcgtct accaaacaac
     1981 acatatcgtt cagcgtaagt cacgtttagg aacgtggctt ttttagtgtt attatcgata
     2041 aataatctat tgactctatt gattttatct ccatatccct acggtgttga ttgtctttag
     2101 atctttcatt taagaaccaa ataactatca taaaaatcag cactgctgct aatataataa
     2161 acagcattcc tattgtgata ttacctattg attttaccat aactacgatt aaagtaataa
     2221 tggcattcaa gtctcctttg ttctctatat ataaattagc cataaatcat cttcctttca
     2281 aattaagata cttgtttgat accaataaat agtaagcgtg gctagcatac ctttttctgt
     2341 aatagcaagt gttctaatag tccaaactta atgatggacc gatgctagtt atagtacagt
     2401 aatgttttct ttcttatcta gatttttagt aagctctatc agcaaatcta acaaatctga
     2461 gtccttttca gaaactaaat taaatctagt gtttacaaca aagttttcat gttctatatt
     2521 gtttagatta tttgcggaaa ggagggcttc tagatcttga cattcaaatc tatatgcatc
     2581 gtcataaatc ttactagaag gagtgcttaa ttcgaaatta cttattttct catcttggac
     2641 caagataaat ttatagatag tatctttttg tgtttttctt ttaaaaacac aacagagtgt
     2701 gttataagga acgttataat tttcatcagc ctcatttttt ctccattcaa acaaatgata
     2761 aactgcaacc aagttgccat caatttcatg gacagtgctc tcggtaacat cctttaaaga
     2821 actatcaagg gctaatttat agtaactaga gagaggaagt aaaattgtcg atttactgag
     2881 atggttatcg tctatacgtg caatcatacc taaatcaact aaggttttat tccaggtgtt
     2941 tatagtttta gttgtcactc ctagagactc tgctatggta ttgaccgaat gccaggaatc
     3001 cccgccttca ttctttgcct taaaacagta atacagatat aaattaatag ctttgttatt
     3061 cggtaaatat ttttcaagtt ctgttggtag aataaagtaa gaatccttaa tgttttttct
     3121 gaactgtctc cagtctctgt agttttgttt ataaagttgt gaaacggttt ttccaccttt
     3181 agaaagtcct gctatcttaa ccaatcttat tgctcctttc ttgaatctgt catgatcata
     3241 cagtattttt tacttgtagt ccagcacctt aatatattat cctatgtaat taatttcgta
     3301 ctagatagta attatttttt atttaattct ctagtcactt acaaataagt tatataaact
     3361 acgattaata aattattaca aaaactatta agaaaacttc tacatacaac tattacaaaa
     3421 ctattaagaa actgaatact cctatttttt gtttttaagg aaagtttttt cttttttgaa
     3481 tttcttattt ttttctaata taactaagca aaactggata atacgacact tattttcaaa
     3541 ttttcacaag actaatagta aatatccatt taataaagtg atacaatgtg ttgattttac
     3601 aaatcaacag aagaagtagg gaaataactt acctatgctt ctttttttgt acatttaatc
     3661 gattcttctt tacaaaaacg aacgtatgtt tgtataatgt ggaagaatgg agtgttattt
     3721 atgatggatt attcaaaaga gccagtaaat gattactttt tgatcgatat gaaaagcttt
     3781 tacgcaagtg tcgaatgtat agaaaggaat ttagatccat taacgactga attagtcgtc
     3841 atgagtcgag cggataatac aggatcaggg ttgattttag cttcttcacc agctgcaaag
     3901 aagaagtacg gtatttctaa tgtcagtcga ccacgagatc taccgcaacc ttttcctaaa
     3961 acgttacatg tcgttccacc acgcatgaat ctctatatta agcgaaatat gcaggtaaac
     4021 aatattttca gaaggtatgt agcagacgaa gatttattga tttactcgat cgatgaatcg
     4081 atcttaaaag tgaccagatc aatgaatctt tttacgactg aaggaacaag aagtcaacgg
     4141 agaaagaagc tggctcaaat gatccaggag aatattaaag aagagctagg attgcttgct
     4201 acagtaggtg tcggagataa tcccttatta gctaaacttg ctttagacaa tgaagcaaaa
     4261 cataatgatg gattccttgc tgaatggacg tatcaaaatg ttcctgataa agtatggaat
     4321 attcctgaaa tgacagacct ttggggtatt ggatctcgaa tgaaaaaaag actcaaccaa
     4381 attggtatca tgagtattaa agacttagct aattggaatc catacactat taagagccat
     4441 ttaggtgtga ttggcttaca gctgtatttt catgcgaacg ggatcgatcg aacagatata
     4501 gcgatcccac cagaaccgac aaaggaaaaa tcatatggta attcacaagt gttaccacga
     4561 gattatacca ggagaaatga aatcgaactg gtggtaaaag aaatggcgga acaagtagct
     4621 attcgtatca ggcaacataa ctgtaaaaca ggttgtgtcc atttatacat tggcacgtcg
     4681 atcttagaaa cgagaccagg tttttcacac cagatgaaga tccctataac agataataca
     4741 aaggaattgc agaattactg tcttttcttg tttgataagt attatgaagg acaagaagta
     4801 aggcacgttg gaataaccta tagcaagctt gtgtacactg attcgcttca gctggattta
     4861 tttagtgatc cacaaaagca aattgacgaa gaaaatttag ataaaattat tgacaagatt
     4921 cggcaaaaat atggttttac gtcgattgta catgcttctt ctatgttaga aggcgctcgt
     4981 agtattacta gaagtacatt ggttggtggc catgcaggcg gcgcaggagg gcttaaaaat
     5041 gattgattat aatgatcgtg gacgtttaaa gtgggcaggt ttttatttat ctgatcatac
     5101 tgaaaaaatt gatgccgaca atgaacaacg agccaatcaa aatttggcaa aagaacaaat
     5161 gacgactgaa gaggttgcgg aagtattgaa cgctgcagtc ataaaaaata gatcagtatg
     5221 tattcaaaaa gaagaacgaa acgctgaagg atactatcca gaagatattg ttagcaagat
     5281 cttaggtagt gatgagctgg ggatctacgt tggaaaaaac aaagttcact acgacgaaat
     5341 acgtcatatc gaattttacc aggataataa atggggtcga ttagaatgaa cattatccag
     5401 caatatgagc taaagtatat aacctttgat cagctatcag aagagatttg gggttatggc
     5461 cagcgtttga taaatgaggt tggtgttgaa cgcttctctt tctatgtaga agctgcagca
     5521 ggataccata aatacgaata ctatattggc tattaaagtg caataaaatt atatttcttt
     5581 tatgttttac attttgtatc aattctgata aaattaccta taggtaatta aaaaatataa
     5641 aaaaactagt tacaacctta ggacgttgta actagcttga agcatactag agctaactaa
     5701 aatagcacta gaaagcaatg ttaaacccgt ccaacaaact tgatttaaca ttgagaccag
     5761 cttattcagc taaatatagt tgaataagct tcttttgttt taaaaaaaca aaaggtgtac
     5821 tacgaataac gcaagtaaaa gaaatactat tacaaccagt aggctacaag taagtattac
     5881 taaacattgc gtagttagaa cctttgctct attgtctaaa gccacaaaat tagagatcag
     5941 ctttatgatg ctaagagcga gctgcatcca tgcggacata ggttcttttg acactattaa
     6001 ttctcctttt taggataagg gaaattgcta gtagtttttt cattagcacc atcccccttc
     6061 atttgattga gtctagtgct gctgtgatag tttgctgttt aatatgctta cattcttatt
     6121 ataacataaa tgaaacaaac acatggttag aggtcgtatc aatcaaaata gtgtctcaca
     6181 aacgatttat tacttactcc tgctgactaa cggactgttc ttcagtaact gtaactatat
     6241 gtttgttaaa accaccatta cctgtagata aaaaccaata tatttactat actcactata
     6301 ttaaatatat ttagtatagt aaaaactaga aacaataaaa tttgtgaatg ttgtataccc
     6361 ccaaacaata cacatatttt gaaggattag gattaataaa gctaaaaata tagtaagtat
     6421 actaaatata tttactatat ctagtatact tactatattt taatcttttg atcgtctatt
     6481 atatataatc cgttcctcgt cgtttaaagt atcaacatag gcgtccattg caagtttgac
     6541 tatttcatac ataggaatat cttttgcata actaagatcc cgaatggttt ttagtacggg
     6601 aggatctact tgaactggtt ttctttcttt cgcagtgtac tttcgttccc ctaactcgac
     6661 attaatatca tctttaattg aaatatcttc agcaccaatt ttcttcttat ctttgttcgt
     6721 tggtaataat gccatttagt ttaatctcct ttccaagagg taggatatga ttggccattg
     6781 agtcagcata tttaggtgta taatcaaatc cttcaatatc accatgttgt tcatagtatt
     6841 caattctttc ttctaattct gtaaagatgt ctgcaaaaat agcccaccat cttcgatcta
     6901 cataatcatt taattggata ccattctcac catacatttc taaccgatcc gagcctttaa
     6961 caatgctttt aaaaatgttt gattcaccat acttatcaat tgttttttga tagtttgcgt
     7021 gttgagagga acgtcgcact gagaaaagaa caggcaaaat tccaatgatt tgaagatgag
     7081 actcttcaaa attcgtgaca atcggaacta aaactttatt aataaaatct tctgttcctt
     7141 ccattgcgta ccgttttaat tcttgcattg caataatata atctgcagca gttaagaatg
     7201 cacgaaccac attatctgtt gatggtgggc agtcgataaa gatataatcg aaattttttc
     7261 tcaattcttc taaaggttta atgaaggcca atgatctctt acgacgatca ggatattttt
     7321 cggacaagta ttcattaagt tcttcagatc caggagagcc agccataata tataaatgtg
     7381 gacttacttg agtaatacct gaaacccagt cccctttttt gattgctgca gacatggatc
     7441 gatcaaagct agtaatttga tacgttgatc caatgttgga agtagtattt ctttgttggt
     7501 cataatcaat tagcaacacg tctttttcaa aaagttgact agaaacaatc gctaataaat
     7561 cattgtctgt agtcttacca atgccgcctt ttctacctgt aagcattaca acttttccta
     7621 gtttatctga tgttttaaat gcagtccgta agttttccat agttccctcc aatttcctat
     7681 actaaatata tttagtatac ttcatatact taatatattc gatatatcta atatactcca
     7741 aattgataag aattcagact ttataaggaa tatagtggat atactaaata tatttgctat
     7801 attcagtata tcagatatat tcaaaatgtc aaaatatatt tgtgcgtttt gcgttgacaa
     7861 tttagagtga aaagaggtaa acttaattct atagaaacca cagataattc actatttaca
     7921 aagtagcaaa agcagtggta ctctttaaac agaaaaatta cataaaaaaa agagacggaa
     7981 ataattgatt ttagcagaat caattatttc tgaatcacat ggttaggaca tgtgttcacg
     8041 ctcgtctcaa gaatatgtaa gtagctacca actacttata ttaaaagttg atcggatacc
     8101 ttttccgatt gcctttattc taccaaaaaa aggcgagcaa ctcaacagaa gaacgtttat
     8161 ttagtgtact taaattaaat attcttaaac actgaagaga gaacacatac tatggctgag
     8221 attaaagcta aactgtgttc tctttttgtt tgagaaagga cgttacaatc gatgttgagc
     8281 gaaatttatt cagctattct taaaaacggc ttacgtacat acaaatatcg aaacagcacc
     8341 atgaaacctg caggtcatat tgattccagt aggagtggtg ctatctttgc gtaccgtagc
     8401 aagcaattaa tgaatgttgg gcgtggtatg gtcattactt cggaagaagc tattttagag
     8461 aacgaaaaga aactaacgca ttggacacct aatacctatc gctttggtac ttatgcggat
     8521 gattatcgaa cagtggttaa aggtcattct gagaaaaact taagtcaaat caacacattt
     8581 gtagttgata tcgattcaaa agagaaccat caaggcgaaa ttattttagc ctgtttagat
     8641 caagtgggct acatgccaac gctaatttta gaatcagatc atggttatca agtttatttt
     8701 gtactaaaga caccaggcta tgtcacaaag aaaagcaatt tcaaggtgat cgatgtagca
     8761 aaaaaaattt caaaaacaat tcgtctacag ttggctgaaa aattgtccgg agtggatcta
     8821 ggatgtaacc acttcggaat tgctcgattc ccacaagaaa ataatattgt ttttaaagaa
     8881 atgaactatc agtattcttt tgctgactgg attcaatggt cgatgaagct tgaatcagat
     8941 caaaaaacaa aagaagatcg agaacgaaaa ctaattgtct ttcctgagaa aaaagaaatt
     9001 cgtcaagtag atgaaccgtg gttcgatatg ttacttcata aagcaaacat cgtcggcgga
     9061 gaaggacgtc ttggtcgaaa taatgtgatt ttcacgcttt ctttagctta ttactcttca
     9121 ggttatgcat tggatacttg cgaatacaat atgttcacat tcaacgaacg gttacaggaa
     9181 ccgctatctg aagcagaatt agtaaagatt gtacgtagtg cttattctgg taattatcaa
     9241 gcagcaagca gagaattcat tattgaattg tgccagcaat gggtttcacc aaccttaaaa
     9301 aataatgatc tgttcattca acgacgtggc tggtggaaat ttaaaaagcc acgagatcaa
     9361 agaaaatata gtcataaaaa tgaatggaaa gaagatctat tggcttatct aagcgaaaaa
     9421 agtcttgcta gtgagcctta tttgaacctg tcaaaaaaag aattggcttc acaactaacg
     9481 atgcccaagc gaacacttga taagatctta aatgagttac gagaggagaa taaagtcttc
     9541 tacaaggtaa cacgaggaag aaatggcgga atgatcattg cttcagtcaa aacgttgttc
     9601 gcgagggtta ttcaactaag taaagaaaag agactagatt attcaacaag gatcacagag
     9661 gtatttggac tcaacgagag cttagtagaa gagacattta gacaattatc aacccccttg
     9721 aaccctctta aagaagtgaa tctttttgaa gtagatacag gttgaaaaat aaaaggtgca
     9781 aattgcctta tacaatatat ctatgatact tctgtaggtg ttgctccctt tgcctagcta
     9841 cgtgtaggtg gtatacttat aactgcgtat gcagtcttat gtatcatgga ggaagttgta
     9901 tgtcgttaaa aaattatgtc tattcacatc ctgttaatgt gtttatcatt ggtcgtttgg
     9961 gattatctgt ggaagtgttc tgtgagcttt atggttttaa gcaggggact ttgtcttctt
    10021 gggttaccag ggaaaaaaca gttgcttctt tgccgattga gtttcttcat gggttgtcct
    10081 tagcttctgg ggagactatg gatcaggtat atgattgttt gtgtgtgtta gagcaggagt
    10141 atattgagta taaaatagct aacgagttaa gaaaaaggaa gaagtatatc cagtagtagt
    10201 ttttatttac ttaaaaaatt ggttcattga gcaacctatc gctgctaaag tagcaaagga
    10261 aaacgttaaa ggaaagaatt tagggaagaa atcgaaatga aatcggacga ctggattgaa
    10321 cacacctggc ttcctctgaa aagaaatagc aaacgaaaga aattttgggt attgcaatta
    10381 tggccattag gaaaactttg tagttacggg gcacgactta ttcttgattt aaccgtttac
    10441 tcgattggag tatttggaac ggcttattac ttgataaatg atcccaatag gattgcagca
    10501 tcagacattt ttacagtgct aatgacgttg atttttgtca tgctattgtc tatttttact
    10561 caattagtca tgcaatttac caggcaaaaa ttaacagcag gaaaactttt caaaagccta
    10621 ttcgatttaa ttgttgtaaa tgcaattcta gcgactttaa taaaacaaat tgaacaatct
    10681 ggaagaacaa caaaaggagt gaaggatttt ttattgacgt ttattcactt tgtaaatgga
    10741 cacatgtatt tgtttgaagt gggtattatt ctcttttttg tcgggtatgt atccacgaaa
    10801 cttattaaac gttcaattga aaaagaaatt gaaattgacc tgattccgac acttcttggt
    10861 tatgagtaca atgttgattt tggaagatat gacgaattga tcaatacgga tatcgttcac
    10921 acgagtatcc atgaatctac tgttaagggc atcagatcta ttttaaaaaa tggccaggaa
    10981 atgaaagcaa tagatcgaag aaaagagatc tacaattttt atgaagtcga agcaagccta
    11041 cgatatggca tcggaaaatt gtggttagat ggaagtaata ttcgtcaaaa caaaccatac
    11101 attttttgga acagtagtga tttagaactt ccaatgaaag ataaataatt atggagaatc
    11161 gtttgaaaat gattcctcta tacaggattg tgcttatagt tggcatggag ttaaaaacct
    11221 taaagaagga gcagttattc aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa
    11281 cgggtacgat gaacattgaa taccgaacag aatatttaga attaacgaat caaactgatt
    11341 ttttgttgat tcgaaagcaa caagagcaag aaaaaaatta atggctactt atagccaatt
    11401 gattgatgaa tgggaacaat tcaaggaatc taaaattcca gaacgtctct aaaagccttt
    11461 atttgtaaag agcataagga aacgtgggaa cactttttaa aaaaagtgtt ccaataggct
    11521 gtgcctatta cgtttctcaa aaataaatgt gggaacaaaa tatttaaaaa taggatagtg
    11581 aaaggatagt gaaggatagt aaaaggaggg tagtaggata gcaaaaagga taggctcctt
    11641 ttcgctatcc tttttaaatt ggaggaatta atatggaagt aagaataaga aatgtggatg
    11701 agaaaatcgt aagagaaatt gatcgaagat gtgacgaa
//
LOCUS       DAZAFF010000082        11646 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000082 DAZAFF010000000
VERSION     DAZAFF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.083"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..435)
                     /locus_tag="ACG9BB_002202"
     CDS             complement(58..435)
                     /locus_tag="ACG9BB_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838130.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(585..710)
                     /locus_tag="ACG9BB_002203"
     CDS             complement(585..710)
                     /locus_tag="ACG9BB_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838131.1"
                     /translation="MKGYLLLKRQLVRHLRNCRFSFLTLDQLSRGKRKIEKDFRE"
     gene            complement(735..1616)
                     /gene="galU"
                     /locus_tag="ACG9BB_002204"
     CDS             complement(735..1616)
                     /gene="galU"
                     /locus_tag="ACG9BB_002204"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFN2838132.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1659..2678)
                     /locus_tag="ACG9BB_002205"
     CDS             complement(1659..2678)
                     /locus_tag="ACG9BB_002205"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFN2838133.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(3311..4315)
                     /locus_tag="ACG9BB_002206"
     CDS             complement(3311..4315)
                     /locus_tag="ACG9BB_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN2838134.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(4328..5377)
                     /locus_tag="ACG9BB_002207"
     CDS             complement(4328..5377)
                     /locus_tag="ACG9BB_002207"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN2838135.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(5394..6215)
                     /locus_tag="ACG9BB_002208"
     CDS             complement(5394..6215)
                     /locus_tag="ACG9BB_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN2838136.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(6202..6981)
                     /locus_tag="ACG9BB_002209"
     CDS             complement(6202..6981)
                     /locus_tag="ACG9BB_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN2838137.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(6998..7468)
                     /locus_tag="ACG9BB_002210"
     CDS             complement(6998..7468)
                     /locus_tag="ACG9BB_002210"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2838138.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(7480..7899)
                     /locus_tag="ACG9BB_002211"
     CDS             complement(7480..7899)
                     /locus_tag="ACG9BB_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2838139.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(7980..10757)
                     /locus_tag="ACG9BB_002212"
     CDS             complement(7980..10757)
                     /locus_tag="ACG9BB_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN2838140.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            10948..11172
                     /locus_tag="ACG9BB_002213"
     CDS             10948..11172
                     /locus_tag="ACG9BB_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838141.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3352 a         2462 c         1895 g         3937 t
ORIGIN      
        1 tctcatctca atagtttatc cattatttta tctttttttg cgtataccta ctaaatatta
       61 ccgtcttctt ctcgttcttc tactttgttc gagagaatct gtactagaaa aagtagttgt
      121 atgtctacct tttctgccta tccgagactt tcttttcata tatgggatat aaatgattag
      181 atacagtaca gcaaaaaata aagcaaatac gcaagcttcc caactaccta acgccaattc
      241 aaccatcaag gtagcaaccc caacggatgc tgcataccag ataaaaatca gaaacgtttc
      301 tttttttctg accatgcgaa acatgataaa tagactaata agcaatagtg gcaacacaaa
      361 tatccaattg cttccttcca tcagccactt gagtatatca agcagcgact gtgcaacagc
      421 gactaatttt cccatagtga acacacacct ttttttataa ttaaaaaatt cgccttttta
      481 ttctactatg aatcttctgg cttggctttc atttctctta tgtaatcatt ttcttttata
      541 caacttaaaa aaaaattaaa ccattagcat aatattttct gaatctattc acgaaagtct
      601 ttttctatct ttcgttttcc acgactcaac tgatctagag tcaaaaaact aaaacgacag
      661 ttgcgcaaat gacgcacaag ctgccgcttt aataacaaat agcctttcat tctcagcagg
      721 ccacttattt ccagttaact ttctgttctt agctttttac tcaactggat aatatatgca
      781 cttaatgaag tcccaatttc cgaatgttga agtccatatt caatatttgt cttcatatat
      841 cctaacttat cgcctacgtc gtatcttttt cctttaaact ctctcgcata gaccgcctct
      901 gtctggttaa gagtctgaag tgcatccgtt agctgtatct cgtttcctgc tcctggttct
      961 tgagtttcta atatctcaaa tattttagga gtcagtagat aacgtccgat aatggctaaa
     1021 ttacttggtg cttttcccat ttcaggtttt tctacaaacc cttttactct gcaacgaccc
     1081 ttttcgctat ctgttttagg atcaataatt ccgtatttag atgtttcatt ttctggtaca
     1141 cgcatgacgg caacagcgga agcttgtgtt tcttcataat ccatcatcaa ttgttgagtc
     1201 aatgggatat catctttcat caaatcatcc cccagcatga cgacaaacgg ttcatttcct
     1261 ataaatgctt ttgcttgaag cacggcatcc cccagtcctc ttggatgaga ttgacggatg
     1321 aagtgaagat tgacttgagt cgtttcttca actaaagcga gtaattctgt tttttccttt
     1381 tcccgcaaat tgttttccaa ttcgatattt gcatcaaagt gatcttcaat gggtcttttt
     1441 tcttttcccg ttacgatcaa gatgtcttcg attcccgaat ccaatgcttc ttccacaata
     1501 aactggatcg ttggtttatc cacgattgga agcatttctt ttgccatggc ttttgttgcc
     1561 ggcaaaaaac gtgttcctag cccagcggca ggaatgactg cttttttgac cttcacttgc
     1621 tgttcctcct aaacgacgat taactattga tcatctattt aacgaagaga aaattcattc
     1681 tccatttttc cgtcacgtaa catgatttct tttgctgctt ttttgatatc ttctccatta
     1741 tacaaaacat tatagattgt ttgagtaatt ggcatctcaa cacctagctg ttgagctagc
     1801 tctactgctg cttttgtcgt tgccacaccc tcaacgacca ttcccatgtt ttctagaact
     1861 tcttccagct tatgtccctg tcctaacaag ttaccggcac gccaattgcg agaatgaaca
     1921 cttgtacaag taacgatcaa gtcccccaca ccactcaaac cgataaatgt cagaggattg
     1981 gcacccatcg cgacaccaag acggctgatt tctgccaatc ctcgagtcat gattgctgct
     2041 tttgcatcat ctccgaatcc taatccatgg atagcgccgg caccaattgc aatgatattt
     2101 ttcagtgctg caccagtttc tacgccaatc acatcattat ttgtataaat cctgaaataa
     2161 tcattcatga acagtttttg tacataggct gctgcttttt gatcaatact tgcagctgta
     2221 attgttgtga tatcatgtac tgccacttcc tcagcatggc taggaccaga taatacaaca
     2281 attgcctgtc ttttttcttc tggaatttct tcagccaaaa cttctgagat tcgtttatgc
     2341 gtatcttgtt ctagtccttt acttgcatgt ataatgattg gcttagtatt cattttacca
     2401 ataagttcct gtgcgactga acgaatcgct tttgttggaa caacaaataa aatagcatct
     2461 gcgttatcca ctgcttctgc caattcttta tatccgataa tcgattctgg gatcgtcaaa
     2521 tcaggtaaaa aatgctgatt tgtatgatac ttattgattt catcaatctg ttcaggtaca
     2581 tttccccaaa tacggacatc atgcccgttt tctgccaatg tttgtgctaa agcagtgccc
     2641 caagatccag gaccaagaac tgcgattttt tgtttcatgg atattcctcc aaataaataa
     2701 ttgataattt ccattatagt atacatttct taaagttggg actaatttcc attgttttta
     2761 agtgtttgtt tacttctttt atcgttttta cttgaaataa tcaatgatat gttacaatag
     2821 tctacatttt atttttacac tcgttatgct ttgcttgttc ctaagaggaa gcagttttga
     2881 caatgttact accagcatta ctattaggag gttaggaaac ttctcctagt gagtattgat
     2941 ttaaaataca taagagacag attgaacatt tctaaatctg tcaggtcctg caacggacag
     3001 attgtctctg ttgacggttg acataggaac ttttgtttta gtcaacattc tggatacaaa
     3061 acgctatgta aactggcgca actcgttgta cttggacttt tactacggat ccatgcaaca
     3121 actaaatagt actattgcat aacaaaaaat ggttggcacc cacatgtccc attaaacaga
     3181 ttataagttg aactgtagat aatgcaaaaa atgtgtttta taacgtaatt gatgccaata
     3241 ttttaaaaaa ataaagggag cagttatcgc tgctctcttt attttttata ttctttttat
     3301 ccagtctatc ctaataatca aactgacgat agtagcggcg tatctctaat ggatggcagt
     3361 tcaactgctc cacatgagca tcgatgcgtt gtgtcactac atgcatcaat aagaaagaca
     3421 aaacacgtcc gcgatatttt tcactgatac cttctactgg atagtctttt gtgtctatca
     3481 atgtataatt gctgcaaatt tttggcaaaa gtttagccac acgttcagat aacactctct
     3541 gttcatcttc gcctaaaaat aatgtgactg gggttgtttc ttctacgatc tctaacgtcc
     3601 cgtgaagaaa ctcggcagca tggatagatt tcgatggaag ccagctttgt tcttcccagt
     3661 aacacatggc atatgaataa acagctcccc attgattccc tgctccaata aaataatgca
     3721 tactatcacg tcgatgtttt tctgcaaatg ataagccaaa tgcatctgct tttttctctg
     3781 cttccactaa ccctgttggt aaataacgtt cgagctgttc atagtattct gaataagctt
     3841 cgaattctcc attcaaatac atcaatcgat ctgctgccat aaaaaatttg atttgttctg
     3901 ttccaggagc tggataagtg accacaaaat cgcatagctg agacaactta ctgttcgctt
     3961 tatcgataaa tccaatcagt gtcgctccta ctttcttgat ttgttctact gctttcacta
     4021 cttcttgagt tgtaccggta acagaggaca aaataacgat agaatctttt gttaatcttt
     4081 tgtttccagt agtgtaatat tctgcagcat gctgtacaaa gacaggcaag ttgcttttcc
     4141 cattcatata agtgacagct tgcatcgagg aagcatacgt tccaccgatt cctagataat
     4201 agacagcgtc aaatttttcc gcataaagtt tatcgatgat ttcttctact tgcggacgta
     4261 aagctagtgc gccttttatt gctttgattt gttcttcttc attgaatttc agcatgattt
     4321 ccacctctta taatttactt ttcaagactg tatcaaagtc ttcaaaaatc tttttgttca
     4381 agtcaatacc tttagctact gcggttttat aagcgaatag ttgaaacgga acgacaagcg
     4441 ctaatacaga cagatattca tcacattcta ttgctaatcc aagtgttttc tcgtttcttg
     4501 ctttttttgt ggtaatggtg agagtctctc caacgtaccg actcatatat ctttgcaact
     4561 tttgcgaacg ttccttattt acactttccc cttcaataaa gaatagcaaa tgcgaagcat
     4621 ctgcctccag gtatgggccg tgcatataag cttctaactc aaatccttgt gacggaacgc
     4681 ggattgtttc agtcaatttc gtctctgctt ctttagctgt tccccagttt ggaccatatc
     4741 caatggtgac gaaacgctgt gctaaacgaa ataaactttg atatccatca aaaaattctt
     4801 ccgttttttg aataatcgct ggtaaatggt tgattatttt gcgcaactgt tgcatgtagt
     4861 cttgttctat ttttttgctg attttatttt tacttattcc aattcctatc cctaagagta
     4921 gtaactgtaa aacagtcgca gaatagcctt ttgtaacgaa gcctactgat tctaccccgg
     4981 cacctaaatc aataatttgt gcagcttcta aagcaagtgg actttgcacg tcatttgtga
     5041 tagcaacaac aggtaaccct tgtttttgta tcatttttac tgcttcaaca gtagaagcac
     5101 ttttcccgct ttgtgacaca gcaatcacag tatcgattga aggagaaagt tttccataat
     5161 tgaaaaaata aaaaggttct tgaatatcga cagtaagatc accataagat tccaatgctg
     5221 gcttcgctgc taaacaagcg ttataagatg aacccgttgc taagatcagt aaattcactg
     5281 tttttttcat atgttctctt gtcgaaaaat cattcttttt caagatcgta cttagtactt
     5341 ccttttcttc atagatataa tcttgcatcg taaacatttt ctctgctcct tttttaaaaa
     5401 atccctatcc acgaaccaaa aattccaaag aaagctagtc cgatcaagat cgttaatggt
     5461 ttcacttctt tcttcaatag ataaaacact atgccaaatg ctccaagaca taagatattt
     5521 ggaacgatat catcaaaaat actttgaaca gtcacagcat tttctcctga accgattttt
     5581 aaaggaatat tgatggtgat catcgtggca gtcattgctc caacgaccat cagtccaata
     5641 atagacgcgc ctaatgtcaa actttccatc ataccgtttg cctggatctt tttcaaaaaa
     5701 cccgttccta agcgatatcc ccaacctgtt gctagatagc gaaacagaat gtgcggaata
     5761 ttgaaaatca aaatgaacaa gatagggcct aaaatatttc cttgcatggc taaagaggta
     5821 cccacacctg tggcaatcaa tcgcaaagtt ccccaaaaga aagaatctcc taaccctgca
     5881 agaggtccca tcaaagatgt tttgatacta tcgatcgcag tcacatcaaa ttcaggatca
     5941 tccttatttt cttcctccat tgcagccgtg attcctaaaa tcagtgtaac gatatgtggg
     6001 gtcgtgttaa aaaactctag atgccgtttc aaagctgctg ccatttcttc tttcgactga
     6061 tataactttt tcaaaatcgg aatcattgca taaacataag ccatattcat ctgacgttca
     6121 taattccaag aaaactccat ttgaaaagaa cgccaaaata cgtgatttag ctcttttttc
     6181 gttattttgc tgtttttgat ctcagaattc ttcatcttct tctacctccc ctgcagttgt
     6241 ttgaagtggt agttgacgga cattcataat attgactacc acaactgcta aaatcgcacc
     6301 aaaaatagct actccggtca caggaatctc taagtaagaa gctatagcaa aacctaagaa
     6361 aaaatatggt gcaacttgtt tgttgatcag caaacgagca agcatcgcaa aaccaagagc
     6421 tggaatcaac cctgtagcta ctgacaaccc cttttgaacg aagtcaggaa tcatatttaa
     6481 tagatttccg atcgtattgc ttcccaccat ataagaaacc attaccacaa caccaagcat
     6541 caaggaaaga ccgataccgg caagcaagtg catatgctcg actcctttat aatttccttc
     6601 ttctgcataa ttgtctgctt tatggttcat aattggaatc aaaagcccta agtaaatatt
     6661 tttcaatatc aatgtcaacg tagcaatcgg caaacctagt aaaagcgcgg tttctgtacc
     6721 ggctccagct gtaatagcaa aagccgtccc taaaatccca ccggtcacaa catcaggtgg
     6781 aatcgaagca cctactgaga acgatccgat aaatgctaac tctaaagttg cgcccataat
     6841 gatcccagtt tttacatcac ctaacacgat tcctgtaaac agccccgtca caatcggacg
     6901 agaaagcaac gatgtcccta gtgcatattc tgattgtgcg acaaatgcaa caattcctaa
     6961 taagactgct tgtacaatca ttgaggttct tcctttctta caaaacttct cttacatcca
     7021 cttttttatc tgctggtact tgacggattt ctacttctat ttgtttgtca attaatcgtt
     7081 tcaagcgttc ctcctcttct ggtaaaatat ttaccgcttt tccgatgctg cgagtaccag
     7141 gttttgcctt gatccctcct aagttgatgg ttttgatttc tggatacgct tctgccagct
     7201 tatatgcatc ctcgatcgat tccactacga tgaataactt atatttatct gtcaccccgc
     7261 ttttcaacgc ggcaatcgag tcatcaattg atttgatcac tagtttgact ccttgtggct
     7321 tggctaattt gatcgttgtc tttcgtatct cattcgtagg tacatcatca ttagcaatca
     7381 aaatacagtc tgcgcctatt gtttgtgtcc atgaaaaagc tacttgtccg tgtaataacc
     7441 gatggtcgac tcttgtcaat acaatcattt ttcttcctcc taaaattcgt cttcttctat
     7501 tttctgattg agccgctcgt tacaaaactg gatcattttt tttgaatcag ttagcacctg
     7561 ctggatcaaa acagttgtct ctacttcagc gtccaaccga gatactagct caatcaatag
     7621 tggcaaattc aatccggcaa cgagatagac atgatcccga ttgatatatt gtaagaattc
     7681 gttgttgaca cttccgccaa aaagatccgt cagaacaatg atttctttat ctttgtacct
     7741 cataaaaagc tgccttactg tttccgctaa atcaaaattc tcatcagtat agcaatcgaa
     7801 tacagtaata gaatgatttt tcccatagat cagctctaat gaatctaata tccctgaagc
     7861 aaattttcca tgggaagcta agataatttc tctatccact ttttcatctc ctcttttcac
     7921 ttacacatac taaaaaagca agttgcatgc caaaattagt ggcaacaact tgcttatgtt
     7981 caaaattctt cttctatcga gagaaactct gtatcttcac tcaaaatatc atagatatag
     8041 gcaatctcag aattggggat catgacacta taatgctctt caatgacact aaacgcagat
     8101 ttaatagcag ataacccttc tttttgacac tgcttccatt tctctaatcc ttgataggtt
     8161 tcgattggca tttgtcggat caatcgttca atgagacagc tcacatggac atacaacgct
     8221 aattttttgg gattgctgat gcttcgattc aactgcacct ctaattctct cataaaatga
     8281 tcgatttctc ccatcacctt ctctgtatct agaatagtca cggaatccag tactttttcc
     8341 aatgaaaaat tacgaatgat gcgctgattg aatatttcct tctctgaagc attcatagta
     8401 gtgtccaacc attcagctag tacagacatc ttttcccccg agatgaggtc ttctaatgaa
     8461 agatagtcga tattggtgat atcaggatct gtggtgccaa tcattcctac gacttcgtat
     8521 atggaaaata atgggtcact gtttttctta tcttttaatt gctgatattc atatgggatg
     8581 attttcaact gacaagccgt aggtaagctt ttttctagca gttcacttaa gtggaccgcc
     8641 gtaccgattc cagtgaagca agtagtaatg agtgctttgg gtttgttttc ctctggatag
     8701 agcagtcgcc attctggctg gatctgtcga acagtctctt ctgctatctc ttctaatgat
     8761 acattttttt gcaggcattc ccctactgtg attgctaaag gtgtcgtcac attattcaaa
     8821 atcaaaagcg gtacggataa ctgtttttta aaaaactgat ggatttcttt cagagatccc
     8881 atgtctacta atatgatcaa tccatttgaa acatcgctgt tttcgcaata atcaaggatt
     8941 tctgaagcaa tctgctgtgg tgtgacatct agtggcatat caaatgattc aaatagatcc
     9001 tcttgtaata aacgatttac tacatttgcc atactgctag cagttgcata accgtgagca
     9061 gcaatcatcg cttttggcag tcgtttttct tttgaccatt tcgctttttt tagatagatc
     9121 gtcagtaaaa tccgatccat acttgtgtct tcaatatcta atttgatctt acttaattcc
     9181 agaattcgtt ggacatagtg ataactagat ggataagact gagaaacttg ttgttctaat
     9241 tctttgatta gttggataat atcatgttct tccggtaacc atttgatagt gcttcgctga
     9301 tagagatagt aactcaaggc atagacactg tttccatcga atttgatttg ataggcagtt
     9361 tccatttgtc gaaaggtctc tcggatagat tgtgtccaat aaaccagcag ctcgtgtttt
     9421 tcttgtctac tggtctcaaa cagcaaaaaa tcaaataatt gaatcacttc ttgctttaat
     9481 tttccttcgc aagaatatag cagacagtta tttttcttga aatcaattag gatttgctca
     9541 aaacttttaa cgattcgctg ctgttcgggc tgttgcttct ccattaattg ctctaaggtc
     9601 tggtcttgcg taatatagat tccttgctgt gctgtatttt tagtaatagg ctgcgcaagt
     9661 agttcttttt gcaaatgatg gatcgtgaca tggatttcag gctgttcacg ctgttcagca
     9721 tatgcttttg ctgctgaaac tttgacgata tttttcagct ctcctatgtt tccggataat
     9781 ttctgacttg ttaaaagtga caaagctggt ccactgactg acaaagggct ccccattttt
     9841 ctttgttctt cgagaaaaaa agagtaaatc agttctagcc gttcattacg actacgctga
     9901 ttaagtgcag gtagttcgat tcggataggt attcttcgaa tgaaagttgt taagaaatga
     9961 ctagtaatct cttcagtcgt agcaaaaaag agacgtgttt tgatccggcg tgacaaatta
    10021 ggttctccca tacgataaat cacaccttga tcaagatagg tgaacagttt ttcctgacct
    10081 tctggtgtta agcgatgtac ctcgtctaaa aacaagatcc caccatctgc tgcttcaaaa
    10141 gccccttttt tattttcttc tgcaccagtg tacgctccct taacatgtcc aaacaaattg
    10201 ctagtcaaaa gttcaggatt atctgcatat tgtgcacaat taaaagtgat gaatggtgca
    10261 gaatcttcta gcagatcatg aagcagacaa tactgataga ccaaattaac taaataactt
    10321 ttacctgtgc cactttcgcc tgtaatcaaa agtggtaatc cgccatctgg atagtaaaga
    10381 gctgttttga tttgttcgat actttctttc aaactggcgt cgtatccgat cataaaagag
    10441 aataagtctt ttggttgcgc gaaaaaaggc tgttcctctt tgagatcatc gatagaaaaa
    10501 taaaaattcg tgcgtaactt aaaaaattgt ttttcaaaag cttctttgtg gaaataaatc
    10561 actggtcgag aattgatttt gactagtagt tgctcatcat ttaactggtt caaataatga
    10621 ctgactgtgt ttctttttac cccaaaatga tcagctaacc tttgtgctgt aaagacctcg
    10681 ttcatatgtt ctaaatcgaa aaaggctgtc tggttctcta agtaacataa tatttccttc
    10741 ttcaacaact tattcacccc tcctccatca tagaaaacgg attcattttg tcaataaaat
    10801 ctttaataaa gaattgaaaa atatatctta ttatagacag cagcctactt atcccttata
    10861 atttttattc taaaaaacgt acagttcctt ccagttttta tactggaatt tcaacatatg
    10921 aaagaataag gaggctatga ctattcaatg aaatcggata aatataaaaa caggaaacat
    10981 acttcctgga aaacttttcg agaaatcatc ggatcttatc tcgctgtagt tgtatttttg
    11041 cctttgcttt atatggctat gacagaacaa actcgtttta ttggcgtaga tacgcaatat
    11101 atagccgaaa caggattttt attcatcttt atcttgattt ttgcgttcta taaatccttc
    11161 catacaaaat aagattcaca gatttcaact ttcctgtctg tttttagcta ttacaaatcc
    11221 ttatatgtgg tataacaaca atttattggc ataatgagga aaaacgagtt atgatgcttt
    11281 cagagagtaa agaggtactg ttgtcaccat cctttttatt gattacgtac accactctta
    11341 gttttttccc attgattacg aaaacaaggt agaaacattg atgttcctac cttgttttct
    11401 ttttttattt tcagatcaaa atctcaagtg gcttttgact tgatgtcggg aggttgtctt
    11461 cgttccatca aacctgatag aatctgcgcc aaaccatagt tttctccact attatttatt
    11521 cctctaagat agtacccaca ataaacgaat aaatattttt ctcattttca tactccttct
    11581 ttgaaaatca attagatttc tctcaaccca cttttttaca aaaattacgt agcaaaaaag
    11641 attgca
//
LOCUS       DAZAFF010000083        11563 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000083 DAZAFF010000000
VERSION     DAZAFF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.084"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..120
                     /locus_tag="ACG9BB_002214"
     CDS             <1..120
                     /locus_tag="ACG9BB_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFN2838142.1"
                     /translation="PTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(735..1775)
                     /locus_tag="ACG9BB_002215"
     CDS             complement(735..1775)
                     /locus_tag="ACG9BB_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFN2838143.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2124..2450)
                     /locus_tag="ACG9BB_002216"
     CDS             complement(2124..2450)
                     /locus_tag="ACG9BB_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838144.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2437..3225)
                     /locus_tag="ACG9BB_002217"
     CDS             complement(2437..3225)
                     /locus_tag="ACG9BB_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2838145.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3593..3883)
                     /locus_tag="ACG9BB_002218"
     CDS             complement(3593..3883)
                     /locus_tag="ACG9BB_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFN2838146.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4019..4198)
                     /locus_tag="ACG9BB_002219"
     CDS             complement(4019..4198)
                     /locus_tag="ACG9BB_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838147.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4195..4545)
                     /locus_tag="ACG9BB_002220"
     CDS             complement(4195..4545)
                     /locus_tag="ACG9BB_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838148.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4538..5863)
                     /locus_tag="ACG9BB_002221"
     CDS             complement(4538..5863)
                     /locus_tag="ACG9BB_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFN2838149.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6313..6927
                     /locus_tag="ACG9BB_002222"
     CDS             6313..6927
                     /locus_tag="ACG9BB_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2838150.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7092..7334)
                     /locus_tag="ACG9BB_002223"
                     /pseudo
     CDS             complement(7092..7334)
                     /locus_tag="ACG9BB_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7538..8857)
                     /locus_tag="ACG9BB_002224"
     CDS             complement(7538..8857)
                     /locus_tag="ACG9BB_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFN2838151.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(8991..9599)
                     /locus_tag="ACG9BB_002225"
     CDS             complement(8991..9599)
                     /locus_tag="ACG9BB_002225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838152.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
     gene            complement(10237..10494)
                     /locus_tag="ACG9BB_002226"
     CDS             complement(10237..10494)
                     /locus_tag="ACG9BB_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN2838153.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10487..10756)
                     /locus_tag="ACG9BB_002227"
     CDS             complement(10487..10756)
                     /locus_tag="ACG9BB_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN2838154.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(10887..11066)
                     /locus_tag="ACG9BB_002228"
     CDS             complement(10887..11066)
                     /locus_tag="ACG9BB_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838155.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            11164..11400
                     /locus_tag="ACG9BB_002229"
     CDS             11164..11400
                     /locus_tag="ACG9BB_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838156.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
BASE COUNT         3550 a         2091 c         1756 g         4166 t
ORIGIN      
        1 cccacgatta aaggcatgga agccatccga ggattatata agaaaacccg aaaagaaggc
       61 actctcttcg ggttttcggt ctgtactgaa atcaaggtat tattgggaat cccagattaa
      121 atcatagata ccgtaaggga ttttattctt tatttaaaac tttgcaacag aaccgtccgt
      181 tgcgggtaat accgtaacac tgaagggcac gcattaaggc gtcatcttaa tgctttcctg
      241 taggacactg acggagatta ccagtctccc gagccttaca ggggcctttc tttttttgta
      301 ttttcaatta tcacaattta tcgtccaaaa gtgcagaacg aaaatcaatt catcacaagc
      361 atatcagaca attaaattta aaacaactaa aaatatacaa aaaagtgtaa ttgaatatta
      421 gcagcactgc ctgaaaagaa gtaatatcag gcttttcagc ttggggaaat aagtgaatta
      481 cctctcttca tgaattatta taactcaaaa aagcacatat gcaaacacta gttccctatt
      541 tttttgaatg aaaaataact aaaatatcta acgattatga ctttaaaacc tacgtttgcg
      601 attgttttca taaactttta atctaaaaac tccggaataa tagctattta tttgtaacat
      661 ttgtaataaa attaaaatca taaaaaagtt ctaatttaaa attgatcatt tttatagtga
      721 tcgcttttta tcaactactc tagccaattt tccgtactaa ctgttggtaa ttcgttaaat
      781 gtttctcttt gttcgtttgc aagctttgcc gcaatagctt tttcgacaaa ccaatttttt
      841 acataaacga aaattagatt ctgcaaattg tttacttttt cagttttttg tttttgataa
      901 gctttcagca aagtcatata taatcctttg tcagcatcaa ttccataaga ttccagttct
      961 tcataaacaa caactgtttg ctctaattct tcagcttttt tctttgcatt atgaatagtt
     1021 tggacggttt tttgagcgtc ttcgtaagta ctagaaaatg tttttatcaa gtttaataca
     1081 ttttctggta caaatgtatt tatactgtcc tctttcatga tgtcgacgaa gttatctagt
     1141 aaaacttgat tttgtaactg atcagtttca gtgtctatta tgtgtctatt agtgtctata
     1201 gaattagaat agaatagatt gtctgcgatt ttcgcaatta cggttgagtc cttgatatga
     1261 ctggattttg ccgtatttgc gattttcgca attacggtgg tatcattgat ttctcgcggt
     1321 tttgccgtat ttgcgatttt cgcaattacg gtggtatcag tattgtcttc ctcttccatg
     1381 attttaaaga cgtcttgatt cgttacttct ggcttgccta agtacaataa attaggcatg
     1441 gattcaatct taccgtcacg tttatttact cttcctctct tctgtttcag aagacctgct
     1501 ttttctaatt cctttttgat tttagatact ttaccttcgc ggcattgtaa caatttcatt
     1561 aattcttcaa ctgtgaatat gaaaaatata ttatcatcag aatctaccca gttatttttg
     1621 actgaataat caaatcgatc tttaagtagc atataagcaa tttttgcatc atttgataga
     1681 tccatgtatt tttcgttagt aaaaaatacc ttaggcattt gataatattt ttcacgataa
     1741 atttcttttg atttgtaata attaaaatca ttcatagcca atcctctttc tgtaggatag
     1801 cgttttgttc aaaactatgt tacaatgaat taaacaaata tatctatcca ctatatattt
     1861 ttctactgct gataagtcgc caaactttgt taaagcagta gtcttttttt atcttttttt
     1921 agtttacctt aaaaaataaa ttaaagcaaa ccgctctatt tcaaggttta agctcatagt
     1981 aactatatca cgatattaca ataatattat atcacgatat cgtaatatta caatatgaag
     2041 ataagcgact tatcagcttt gatactactg atttttataa aaaaaatatc acgatattat
     2101 gatatcgtga tattgaatgt aaattaaatt ttattttgtt tctttgctag tttatatcct
     2161 tcaatcaagt attcttcagg tatggacttt aatagcgcat cttctataac gtctggttta
     2221 cttttgtctg attcagcaat agtcgtccaa tatttgagtg ctgacattag aattggattt
     2281 atacgaatgc tatctgctgt tcttacacta cttccttgat cttttatgac tttacttata
     2341 tttttcttgg ttaattcgat ttcttctact tcttttgatt taaaattctt tttattttta
     2401 tttcctagta atctacttac tgcagcttga ctgcgattat cttctgccat caaaatcacc
     2461 aacttctttt aaatattctt ctgtaaatgc tttatagtca tcttcagctg ttgagggttt
     2521 attaaaattg attagatctg atccagcagc ttgcgcttcg tcaacggtta ttgtcctacg
     2581 aatataagtt ttgaaaattt ttgcaccaat gactctagaa atttcggttg ctgcagacgt
     2641 catttcttta gaaatgttat aacgcttatt atacttagta aatagaactc ctcgtacttc
     2701 aagcttgtcg ttcgtatatt cttttaccgt ttcaatagtt tcacttaact ttattagtcc
     2761 ttttaatgag aaatacgcag cttcaactgg aatgataatt tcatcagaaa cagtaaatgc
     2821 gttgactgta agcattccta aacttggtgg acaatcaatt ataatataat catagttgtc
     2881 cttgattggt tgaagtactc ttttaagcct gtgttcactt cctacttgag taaattccct
     2941 ctctgcagaa gaaagggcta tgtcggctgg caaaacatca attcctttat agtccagaat
     3001 agcatctgta atgtcagcct ccccttttaa tacttcataa attgtaggtt ggtttgttcc
     3061 atctatatcg aaacctacat tatctgtagc atttccctga ggatcaatgt ccactaaaag
     3121 tacattttta ccgtaaacaa atttcaaaca tgctgctaaa ttaacgctag tcgtagtttt
     3181 agaaactcca cctttttgat tggctactga caatattctt gccattaaaa tatcccccat
     3241 tcatcttata ttataatata attatatcat aatattgtga tattgcaata tcgtatatgc
     3301 attatattgt aatattgtgt gattattttg ttttaaagat atatatcggt tcggatataa
     3361 agattattca tatgttattc gaaattaaaa taatcacttt ttattacttt aaaatttttt
     3421 attaatgata agaaatatgt tttgcgagca gcaactagaa taatttaaac attactaaac
     3481 tgtctatatt gttactaata attcccttct tatagtgcat tttcttagaa aaaaacaata
     3541 ttacgatatc atgatattaa gatatcgtaa tattatacaa ttgtgattga tattatatat
     3601 gtttttttat ttttttatca tgtttaatct tgaaggaatc atattctttt tctaaagaca
     3661 ggagcttttc ataaacatca gacatgttta aactagaggc caggcttaaa tcataaacga
     3721 atgaagcagg taatgatttt attctacgtt gtcgggttat ccagcttgca accgttcctt
     3781 gtgggtaggc atataactca caaaattgtt ctacagtgat tcctaaattc ttgataatgt
     3841 aaacattgat cgggtgcgag taaacaaatg tattgataga catggtgcgt aagcctactt
     3901 tcttttatta ttgcgtttgc aataatagtt taacacacgc attattctag tatctactcc
     3961 tatttgtaaa aacaaaaaag gattcataga gataactcta tagaatccgt aaaatacctt
     4021 atatatataa cgggaagata taaaacctaa aattatgata acctgctgca gcttctacat
     4081 agaaagagaa gcgctcaaca ccaacctcat ttatcaaacg ttggccataa ccccaaatct
     4141 cttctgatag ctgatcaaag gttatatact ttagctcata ttgctggata atgttcattc
     4201 taatcgactc catttattat cctggtaaaa ttcgatatga cgtatttcgt cgtagtgaat
     4261 tttgtctttt ccaacgtaga tccccagctc atcactacct aagatcttgc caatgatatc
     4321 ttctggatag tatccttcag cgtttcgttc ttctttttga atagatactg atctattttt
     4381 tatgactgca gcgttcaata cttccgcaat ctcttcagtc gtcatttgtt cttttgctaa
     4441 attttgactg gttcgttgtt cattgtcggc atcaatttct tcagtatgat cagataaata
     4501 aaaccctgcc cactttaaac gtccacgatc attgtaatca atcattttta attcccccat
     4561 ttccgcccgc atgaccacca acaagtgtgc ttctagtaat gcttctagcg ccttctaaca
     4621 ttgaagaagc atgtacaatc gacgtaaaac catatttttg ccgaatcttg tcaataattt
     4681 tatctaaatt ttcttcgtca atttgctttt gtggatcact aaataaatcc agctgaagcg
     4741 aatcggtgta cacaagcttg ctataggtta ttccaacatg ccttacttct tgtccttcat
     4801 aatacttatc aaacaagaaa agacaataat tctgcaattc ctttgtatta tctgtgatag
     4861 ggatcttcat ctggtgtgaa aaccctggtc tcgtttctaa gatcgacgtg ccaatgtgta
     4921 aatggacaca acctgtttta cagttatgct gcctaatgcg tatcgctact tgttccgcca
     4981 tttcttttac caccagctcg atttcatttc ttctagtata atctcgtggt aacacttgtg
     5041 aatttccata tgatttttcc tttgtcggtt ctggtgggat tgctatatct gttcgatcga
     5101 tcccattagc atggaagtac agctgtaagc caatcacacc tagtctattc ttaatcgtat
     5161 aaggattcca gttagcaaga tctctaatgc tcataatccc catctgattg agtctttttt
     5221 tcattcgaga gccaattccc caaaagtccg tcatttcagg aatattccat actttttcag
     5281 gaacgttttc gtacgtccat tccgcaacaa atccttcatt atgctttgct tcgttgtcta
     5341 aggctagttt agccaaaagg ggattatctc cgacacctac tgtagcaatc aatcctagct
     5401 cttctttaat attctcctgg atcatttgag ccagcttctt tctccgttga cttcgtgttc
     5461 cttcagtcgt aaaaagattc agtgatcggg tcacttttaa gattgattca tcgatcgagt
     5521 aaatcagtag atcttcatca gccacatatc ttctgaaaat attatttacc tgcatatttc
     5581 gcttgatata taggttcata cgtggtggaa caacgtgtag tgttttagga aatggttgtg
     5641 gtaagtcacg tggtctactc acatttgtaa taccataccg cttttttgct tcaggagaag
     5701 aagctaatat caatcctgaa ccagtattat cagctcgact cataacaaca agttctgttg
     5761 ttaatggatc taaatttctt tctatacatt cgacacttgc gtaaaagctt ttcatatcga
     5821 ttaaaaagta atcatttact ggctcttttg aataatccat cataaataac actccattct
     5881 ttcacattat acaaacatat gttcgatatt gtaaagaaga aatagctaaa tacataaaaa
     5941 aagaagcata ggtatgctat caccctgctt cttcttttga tttgtatgat caatttattc
     6001 tatcacttta ctaattaatt tgaaaatcta ataaaaactc tcactctaaa agagtgagag
     6061 actgcaaatt agagtacgga cttttttgga gttccgttct ttaatattaa caaatgaata
     6121 aaatccttac aacatttgag aataagttat cagtagacac ttacaaaaaa ttctcatact
     6181 atttcctcca tcattagttt ttgtctatat aaggtttacc ctaaatagac actggtataa
     6241 cattgaaaaa tatggcttcc tcgtgtatga ttagggtgta ttttaaatag ttgcagaaag
     6301 taggtaaaag ttttgagagt tgcatacata cgtgtctcat cgatcgatca aaatgaacaa
     6361 cgccagattg aagaaatgaa aaaattcggt gcagagagaa ttttcatcga gaaacaatct
     6421 ggcgcaacga ttactcatcg acctgttttc caagaagcat tagattacgt ccgagatcaa
     6481 gatattttta ttgtggaagc tattgatcga cttggaagaa attatgacga gatcatcgct
     6541 tcagtcaatt acttgaagaa gaaaaatgtg aagctgatca ttactagttt accgatcatg
     6601 gctgaagcaa ttggcaatcc tctcctggat aaattcatta aggatctgat catccaaatt
     6661 ttggccatga tcgctgaaca agaacggact gaatcgaaac gtcgccaggc acaaggaatt
     6721 aagatcgcaa aagcaaacgg cgtctacaaa gggcggccca aattatacag tgcagacgca
     6781 aaagatcccc aacgtcgcct tgtttacaaa agcatcgttg aagatcttaa aaatggtgtt
     6841 gcgattgcta agattgctaa agactacaat gttacgagac aaaccgtgta tagaataaaa
     6901 aaagacagca tggtcaatga caaatagttg atcatgctgc ttttattatc ttctcatctt
     6961 taatttccta catataatta ctatctataa aattaagtct tactcagaac agataaagca
     7021 taaaattcca acaaaccatt ttttacttaa ctaaataaag agtctgttaa tataatcttc
     7081 acttatttac gttaatcccc tactgtattt gttggttcct tatctatatt ttctacaaaa
     7141 tccttattat cttgtgattt agcttcgtat aattctttgt atgtcgacac attggttata
     7201 ctaattccta tagctagttc ttgagactct acttttctct ctagctcctt tgacaaagtg
     7261 atttgagatt tatttggttc cgttggagtc aattctatag attcttcata aaaatctcta
     7321 tacataaagc tatccctgaa taattcatag ctttaattct ttggcacatt ttattgaaat
     7381 atgtatcact aatatcccta tcaactgatt ttgactaaaa agttacttca aacattcact
     7441 gttcaaaaat attgaggaat aaattttggg tgtaaggcat tttgagcata cttctccctg
     7501 gataacttcc cagtaaaaaa atcgttagat tgttggatta gatccattga ctggctcgcc
     7561 tcaggcgagc aaagaccctg tagaattcat tctgatacac atgataacta gataatttga
     7621 gatagactcg tctacccgtt tggactaatt tcccagcaac tttcagaaac ttcaatcgaa
     7681 tagaatgaat ggtcattccc ttttggactt cttcaaagcc aatcgttctt aagaagttga
     7741 ctaagttgta agctatcaag ctgagcatca ttcggacatg attctccaaa aagcgaggac
     7801 tgtctgtttt gtcaaagtaa aagccagctt tcgcttcttt aatgaagttc tccattgtgc
     7861 cacgtttggc atagatagaa aagatggtat caggagaaac attttctgat agattcgtca
     7921 cgataaattc atgtcgaaag agtagttcgc ccgcttcacg tgttgagcgt atacacacgc
     7981 gacgactttg tgaccaggat tgtgcttgat aagtggtcga gaagtactga acttctcgtt
     8041 cttcccactt ttgattatcg ccataaagta ctgatttctc agctatttga cctagtctac
     8101 gattattctt cagtcgaata acataatgac tcttttttga ttcacacgaa tcatacacac
     8161 caggtgtagc gaacccgctg tctccacgaa ccaagatgtc agtgtttggt agagagtgat
     8221 tatagtgctc taataaaggt gtgagaaact cctttacgcc ttttgatgta tattgatttc
     8281 ctgaacgtag ttcagctttt aagaagtcac cagtcaatcc gtcaaaagct accaatggat
     8341 ggtaaccata ggtttggtag tgtgtattat aattcgtttg ttcttggtga ccaaatgtat
     8401 ctgaatgggt cgaatctaaa tcaatgatta attccgtatc attacggatg aggcgtgctt
     8461 tatcgataag ttcttggttc aaagcttgaa gttcatggat attctcctca gatagtcgat
     8521 ctaaaaatcg ggaaagtgaa gattgagaag cgagttcttt cctatctaaa acagctttaa
     8581 acacaggatc ttgtctcagg agattagctg cagaatcagc tgagtaacca gcaattaatt
     8641 gcataatgaa ctgctcaagt atggataagt tgtcatgagt aaaatacgct cgttcgtctt
     8701 caatgtgaat gtgttgctta gccagttcag aaaaaccaaa gatgttcatc agctctttca
     8761 ctaggacgag acccgaatca ctcgataatt gaccacctgt atgagaaata gtgatatttg
     8821 aattgaattt tacttggttt ttgtgtaagc tagtcatgaa aagaactcct ttcttttggt
     8881 tggtttagtc actttaacca tagcagaaag gggttctttt ttcatcactt aacgggtgaa
     8941 gatgaaaaag taattgtaag ctgccgtgag gcagtttcac atggttttaa ttaaaagtat
     9001 gaattattca ggctatcatt aattttataa aaaaaattaa atgtttttag cactgccata
     9061 taacattcaa aacattcttc tatagttggt ttacttcctt gaggagaatg aaccaattta
     9121 ttacgttttt ctatgaaatt tttaatttcc tttcttattt ctttcggaaa ctcaatatta
     9181 aagtaccctt taaacatgct ccctaataat ttgtctacag ggggagattg taaattctca
     9241 aatattagag ttaaatcagg tttctcttgt tgataaaatt cctttacccc tatttcacag
     9301 gctgtaatta tcgaaataaa cctatctcta gtatttgata tatttttact tcttttaagg
     9361 agtataagag aaaaggggat actttcgtgt ccatgcatgt aattaactaa ttgttgagct
     9421 tcagaagtta tatctctaat tatatagtta acggaattag gaagtgtctt taactctaat
     9481 gaaatttcta tccaattact atcttctaat ctgtatatgc tcgaagtata atttgattcg
     9541 tcagcaagca tcgctacatc ccaaacactg atcataaaat cttttaaacg ttcaatcatt
     9601 tcagcaatat tgctaaaatc agctaatttt tctttagaat aaaaaaaaac atttatatca
     9661 gaatcaataa attctatttt tttattacta tatcgtcatc attatcttga ccatttatgt
     9721 ttattgttcc ctgagtgggg agtacttctt tcaaattcaa gttaaattta tattccatta
     9781 cttctcctcc aatatataaa aattacttaa ctgtatccaa atttaatatg taaataaatc
     9841 attaattttt agctaaattc tatgagaagt ttatttttgt tgtttttgct aattaactaa
     9901 aactagtaca aaaaatttaa aatagattat taaacctatt gcttattcta ttcagtcata
     9961 tatataagcg tcatccttta gtaactaaca tcccacacta caatagtatt ataacatatc
    10021 tattcaactt caaattgttg aaatttaagc catttatatt tacttttcct caactttaga
    10081 aatgatttta ctaaccttat atccgtaata cgcgtaacaa acacattcat taataacaat
    10141 caacactaag ctatattagc tttttaaatg actagggtaa ataaaataag gatcaaaaac
    10201 ttttaagttt ctgacccttt ccttacttcc gattggttaa tagtgatctt ttgcagaata
    10261 aataaatatc gtttcatttt caactctata tatcagtcta tgttcatctg taattcttct
    10321 ggaccatttt ccagataaat catgctttaa tggctcaggt tttcctaatc cagcaaaggg
    10381 ggaacgatcg atatctttta ttaacttgtt aatctttttt atattgcttt tgtttccttg
    10441 ctcatgccaa taaagataat catcccaagc atcatcagac caagccttaa tcatcagatt
    10501 caacctcgat taagtcatgt gttttaaatg cacctttgga gaattgttca tctcctcgac
    10561 gaattttttc catgacgtaa ttattagaaa gtgttctcaa cgtttcttgc atagaatcat
    10621 aatctctttt tgataataca acaactgtat cttctacatc tttacttgtt acaataagtg
    10681 tttcagcatc ctcattaact tgtttcatat aactacgtaa attttggcgg aaatttgaat
    10741 aagctactgc ttccattcct ttcacccctt atttatttct ataaaacaat tgtacattat
    10801 attgtacaat taagcaaatg attaatattc taacttctta taattaaaag tgctatcact
    10861 tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc aaatcctgct
    10921 ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc acttttgccg
    10981 tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt aacgggtaac
    11041 cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa taaccctatg
    11101 aaaaatatgg ctgcaatcat cttgaatagt agactcacaa gggctaatac aaagcgccaa
    11161 aagatcaaac aaaataccag tattgccaaa aatactacga agtttaaatg cacagctgca
    11221 gcatggcgca taagtatttc gccaacaaat aggactaaca atagaggaat gattttattc
    11281 atatggatct ccttctttct cttaggttat cgggtgttgc ccgcttattt cgcaccacct
    11341 aagcggcggg ggcggttgga gcgattggtg cgaaataaga accattccct tagttactag
    11401 gttttttgtc cttatttcac acattctcac ttttcggtat aaatgattat tgcctttttt
    11461 tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg atttcttctg
    11521 tatcgtcgta agtataggtg tctaaaaaat cagttaaagt cgt
//
LOCUS       DAZAFF010000084        11541 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000084 DAZAFF010000000
VERSION     DAZAFF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.085"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(113..718)
                     /locus_tag="ACG9BB_002230"
     CDS             complement(113..718)
                     /locus_tag="ACG9BB_002230"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN2838157.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            914..1837
                     /gene="coaA"
                     /locus_tag="ACG9BB_002231"
     CDS             914..1837
                     /gene="coaA"
                     /locus_tag="ACG9BB_002231"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFN2838158.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            1896..3461
                     /gene="guaA"
                     /locus_tag="ACG9BB_002232"
     CDS             1896..3461
                     /gene="guaA"
                     /locus_tag="ACG9BB_002232"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFN2838159.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(3952..5583)
                     /locus_tag="ACG9BB_002233"
     CDS             complement(3952..5583)
                     /locus_tag="ACG9BB_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFN2838160.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(5597..7270)
                     /locus_tag="ACG9BB_002234"
     CDS             complement(5597..7270)
                     /locus_tag="ACG9BB_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFN2838161.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(7283..7600)
                     /locus_tag="ACG9BB_002235"
     CDS             complement(7283..7600)
                     /locus_tag="ACG9BB_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFN2838162.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(7625..9025)
                     /gene="lacG"
                     /locus_tag="ACG9BB_002236"
     CDS             complement(7625..9025)
                     /gene="lacG"
                     /locus_tag="ACG9BB_002236"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFN2838163.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9262..10047
                     /locus_tag="ACG9BB_002237"
     CDS             9262..10047
                     /locus_tag="ACG9BB_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN2838164.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            10050..10742
                     /locus_tag="ACG9BB_002238"
     CDS             10050..10742
                     /locus_tag="ACG9BB_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN2838165.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(11016..11387)
                     /locus_tag="ACG9BB_002239"
     CDS             complement(11016..11387)
                     /locus_tag="ACG9BB_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFN2838166.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         3496 a         2478 c         2107 g         3460 t
ORIGIN      
        1 agaaaaaata gttctttgtt tttaaaaact tctcaacaaa aaaagaactg atacgtaccc
       61 gtacatgcac gcccagttcc actctttcgt cgctccacta tttttcagct agttattctt
      121 tcacactttt gatgatataa tatcctttat cttttgtgac gatttctgca ttaccaaatg
      181 tttcttccat tttcttttgc gcactcggcg ctccttgttt cttctggatc acaattgtca
      241 gtgtaccgcc cttcttcaac cgaggatatg cgcctgtcaa aatttcatgt acaacttttt
      301 ttccagcacg gatcggcgga ttgcttacga tagctgcata ggtttcttca tgaactgctt
      361 catagatatt cgattgatgg atatcaacat gttcgatttg gttacgcttt gcgtttcctt
      421 gcgctagact taccgctcgt tcattaatgt cgaccatttc aacaaaccgc tgtgtcgcaa
      481 atgccaaagc taacccaatc ggtccatacc cgcatcccac atctaagatc tttccttcat
      541 caggtaactc ttcccaatcg aaagcatcaa tcaggacacg tgaaccataa tccactgttt
      601 cacgtgaaaa tacaccagaa tctgttacaa attgaaaggt tctgcctttt aactcaaaag
      661 cccattgttc aaaatcgtgc gccgtatctg ggcgttcaga atagtaatga tttgtcatgt
      721 cttccatcct tctcatttct ttcatgacaa cagtctatcc ttttatgtat ccgtgcgcaa
      781 gtcaagaata agactccgtt tctttctatt ataaagatta tacaaaaaga tcggcaatct
      841 ctcttaagtg gtggggtttg cgtttatttt gtgttaaaat agttccattg atgggagaac
      901 gcaggaggct aatatgaatg atccaatgaa ctactaccgt gtggcaagag aagaatggcg
      961 agaattttat cgtaatgggc aagcaccttt gacacaagat gaactagata atatcaaaag
     1021 tttgaatgac cgcatttcca tgcaggatgt tcgagatatc tatgtacctc ttgctcatct
     1081 gatttatctt tatatgaaag aatttgaatc tttgttcttg agtaaaggtt tatttcttca
     1141 taaatacgta gaagtaccgc cttttattat cgggatcgcc ggaagtgtag ccgttggaaa
     1201 aagtacaaca gccagactgc tgcagacgat tttgtctcgt accttcaaac gaagaaacgt
     1261 tcagctgatc acgactgacg gatttcttta tccaaatcgt atattaaaag agcgtggaat
     1321 catggaccgt aaaggatttc ctgaaagtta tgatatggag atgctgatcg atttcttgaa
     1381 ccgtgtaaaa tctggtcagg aagagataaa agtacctgtt tactcacatg atgtgtatga
     1441 tatcattcca gacaagtacg aattgatcca gcagcctgat atcttgatcg ttgaggggat
     1501 caatacgctt caattacctg cgaatcagca aatctacgtc agcgactttt ttgatttttc
     1561 tgtctatgtc gatgccgatc ccgtgctgat cgaaaaatgg tatttggatc gttttggtgc
     1621 gttactggat acggctttcc aaaatccaga gaattattat tatcgttttg ctatcggaga
     1681 tcgtgaagaa gcctttaaaa tggcaaaaga tgtatggaaa aatgtgaatt tgaaaaattt
     1741 acaggaatat atcttgccta cacgaggaag agccgatata attctccata aaactgaaaa
     1801 tcatatcatc gattcgattt tcatgcgaaa atattagcat tttaaaatag tttgactatt
     1861 ttgagataaa atttaaaggg ataggggtaa taaatttgac aaacgttgcc gatatgacga
     1921 acgttgaaaa gattatcgta ttagactacg gtagtcagta taaccaattg atcacaagac
     1981 gtattcgtga tcttggagtc ttttccgaat tattgagcca ccgcattact gcagaagaag
     2041 taaaagcgat gaatccaaag ggaatcattc tttctggcgg accaaacagt gtatatgatg
     2101 agaatgcatt tgggatcgat cctgagatct tcaatttagg tattcctgta ttagggattt
     2161 gctatggtat gcaattgatc acgtacaaac ttggcggttc cgtagagcct gctaaaaacc
     2221 gcgaatatgg gaaagccttg ttagaagtaa caagcgatca agctgtcttg tttaaagaaa
     2281 caccaaaaca acaaacagta tggatgagcc atggtgactt ggtaactaaa gttcctgaag
     2341 gctttgaaac agtcggaaca agcaaagatt gtccgattgc ttctatccaa gacacaacac
     2401 gtaattttta tggtatccaa ttccatgcag aagtacgtca ttccgaatac ggaatcgaat
     2461 tgctgcgcca ctttgccttt gatgtttgtc actgtgaagg taactggaca atggataact
     2521 ttatcgatat gcaagtagaa aaaatccgtg aacaagttgg tgacaaaaaa gtcttgcttg
     2581 gtctttcagg tggcgttgat tctagtgtcg ttggggttct tttacaaaaa gcgatcggtg
     2641 accaattgac atctatcttt gttgaccacg gtctgctacg caaaggcgaa gcggaacaag
     2701 taatggaaag tctaggcgga aaattcggct tgaacatcat caaagtcaat gcaagagatc
     2761 gcttcttaga caagttgaaa ggtgtatctg acccagaaca aaaacgtaaa atcattggga
     2821 atgaattcgt atatgttttt gatgacgaag caacaaaact tgctggtgaa gaaggcgtct
     2881 ctttcctagc acaaggaacg ttgtatactg acgttatcga atctggtaca gaaacagcac
     2941 agacgatcaa atctcaccat aatgtaggtg gactgccaga agatatgcaa tttgaattga
     3001 tcgaaccatt aaacacatta ttcaaagacg aagtccgtga attagggact cagttaggca
     3061 tgcctgattc aatcgtttgg cgtcagccat tccctggtcc tggtcttggt attcgtgttc
     3121 ttggcgaaat cactgaagac aagctagaaa tcgtacgcga atctgatgct atcttgcgtg
     3181 aagaaatcgc agctgctgga cttgaccgtg atatctggca atatttcact gttcttccag
     3241 ggatccgctc tgtcggtgtt atgggtgacg gacgtactta cgactacaca gtcggtatcc
     3301 gtgctgtaac ttcaatcgac ggaatgaccg ctgactttgc ccgtatccca tgggaagtct
     3361 tgcaaaaaat ctctgtacgt atcgtaaacg aagtagccca cgtcaaccgt atcgtgtatg
     3421 acatcacttc taaaccacca gcaacagtgg agtgggaata aagagccaac ataatatgga
     3481 gggggaatcc ctataaatca acattcttca atagctaaaa tgcttgattt tcattaaaaa
     3541 taagatggtt ccctaccaaa agagactata atataagtta atttgtttca ttcttatcat
     3601 aaaaagaagt tctcgaaaaa cgaactgttt tccgagaact tttttctatc catctaagtg
     3661 ctcaaatcat attcacgacg gaattctaag gttacccttt cttaaacaaa ctttatgtta
     3721 tctgcacaac tttttgcaat tttttcattg cttcttacga aatcgttgaa ctctctttag
     3781 cataatacaa agtgtagatg aagtctggtg ctttactcgt tttggtaaaa atgactggaa
     3841 atcgtttctg gttatttttg gcaaaggcga ggtgcaggtt cgattcctgc cttctacgct
     3901 aacgatgtgg ctgaagggaa cctccgatct tgaatcgaaa cgatccgcag tctattgttt
     3961 caccaatgaa acaaaatcaa cgttcgtcca agcatttcct gaagccttgc tataaacccc
     4021 aatttcacaa acacctgttg taattggaat gtctttgatt gtaatttttg tccattcact
     4081 tgttttaaca accgcagtat ttagttccgc tccaccataa tttttgatat ataactgatt
     4141 agtatcaaac tctcccccag acattacata ggcactcaat gtgtaagttc ctttgcttat
     4201 gccactaaca ttttgataag ctgatgcctc gtatgcttga tcctgccaaa aagttagttt
     4261 gtagtcccca aaaaaagtgc ccgcttccgt tttgatcgta cccttagcgg ttccttgttg
     4321 aaaccaggtt tgccaattgg aaggactggt cgttggtcca tcgctttcga aacttgcatt
     4381 tttaactaga tttatttgcg cccctgcttg tttaaacgca cgcaagcttg gcaaggcgtt
     4441 tccagcaaaa tcaaacatcg cttggttgtc ccattcgttt cccatagtgg attgaacact
     4501 gagataattc atacctgctt gtgttgccca actcacattt ccgttgtacc aaagaggttc
     4561 ccaatagaag aagcccgccc cgcgattatt cggtactgcc tgaatccgat cggcaagatc
     4621 cagcaaatac tctgcttgtc ctgtcggtgt tggttcatat ccccccgcag ctgcttcggc
     4681 ttcaccaaaa gcatttggca tagtatcggc attcgctgtt gtatgcccat aagctgtttc
     4741 aactacgatc acttctttac cataccgttg gctgatcgca ttcatattgg tttgaagttc
     4801 attcattgaa ccgtgccaat acggataata cgacactcca attaaatcaa atgacacacc
     4861 tcgactcgtg atttcatcaa accaccaagt aaatgctcct gtatcgccac catcagctaa
     4921 gtggagcatt actggagttg ttttaggttg agtgtctcga acagcttgga taccagactt
     4981 caaaaaagca gccaatcgat caaattctcc cccgccttct ccccaagact tgccataagg
     5041 ccagagcatt ccgctattca gttcattgcc gatctgaacc atatcaggat aaacgtccag
     5101 ctgcttcatt tccgacagaa cgttctttgt atagtcgtac aatgccgtat tcatttcttc
     5161 aaaggtcaga ccttgccatg ctttaggaag attctgtttc ccaggatcaa cccaaaaatc
     5221 actgtagtga aaatccagca gcaccttaag tcccagattt tttgctcgtt ttgcaaggtc
     5281 aatcgtcgtt tccagatcat ttgtccctgc tccataactt tctccattac tattataagg
     5341 atcctgccac aaacgcagtc gaacatagtt gacaccatta ttttttaaaa tctctaaggc
     5401 atcttttgaa atatcgtctt cataatattt tgcaccagat ttttccatat ctgacaggat
     5461 cgaaatatct gctccgcgaa taaattcgct ggcagatgct tcaacagttt ttgaaacagc
     5521 ccccaaagcc cctaacacag ctgcaaataa aacagcttta cgcaaccaaa ttcccatttt
     5581 cattatggct cctcctttat tgaaattggt tcagaataaa ctgaacagct ttttcaggat
     5641 ctctcgttaa tccgatatat tctgctcctt ttgtcgcaac cagcttgatt cctaagcgat
     5701 cggtgtcttc tttgatatca tcatagtaag aattgacttg cggtgctaag accaccatat
     5761 caaaatcctg cagcatttcg taatgagaac cataagcacc cgccattgca ctgatcggtg
     5821 ctttcgattc agatgcacct ttttttaaag cattagctaa catcccactt gtccctgcgc
     5881 cagcacaaag taccaacaca ttgatttctt tatttgcatc gctgataact tgactcgctg
     5941 ccacaggtac ttcctcacgt acatcggcag aagcaccttc ctcggttgcg atcacctctg
     6001 ctttttgtgc ttctttctct actaagacag catcgtatgc tttgcaaaat ggtagataga
     6061 tcaggaagtc aacgacgatt agaaccgctg ctagaacaaa agctaagata cccatgcccg
     6121 ttcctagaac caaaccgatc ggtgctggag ttgcccaagg caaaacgtac ataaagctgt
     6181 tcatgcccaa tacatcgaca aaaaacttga acaggcaaac gtttacaaca ggtgttaaca
     6241 aaaatggaat aaaaaaataa gggttcaaaa tcatgggtgc cgcaaatagc aatggttcgt
     6301 tgacagcgaa acttactgga ataaaggaag ctttcccaac tgccttcaac tgcttagact
     6361 tagcaaagag taggaagata aaaggaacaa ccaatgttgc cccagttcct cccatagttc
     6421 ctacaaaatt ccctaaaccc accgtcagta ctttagaagc ttgttcaccg gaattgaaca
     6481 attgcagatt ggtttccaca ttggcataga taattgccgc aattgccggc tccacgatcg
     6541 acggtccatg aacaccgaca aaccaaaaca gcgccatcgc tccccagata atagcgattc
     6601 ctaagtaccc gtcagctgct gtaaatagcg gctgcagcac cgtaatgatt gcttctgcaa
     6661 atgagatttg gaagatttgt cgaatgccta aatcaattaa aacggccgca aatactgaaa
     6721 aagagaaagg aaaaatatct cggaaggttt gcgaaatcgt tccaggaact tctttaggca
     6781 ttttaatggt aatatctttc agcacacaaa acttatataa attaactgtt aaaaatgcag
     6841 cgacaaagga agccagtagt ccctttgtcc ccatatattc cgttgaaaat ccaccttcaa
     6901 tcggagagac gcttaacaac aggaaactta ccacagaggc cagcatcaca gaggtggtgt
     6961 tgattacttt gtttttcggt aagcgacgat tcatcgaatc agccaagcag cgagcagtgg
     7021 ttgctgaaac gagtaaccct acaacaccca tcgaataatt atagactttc catagccatg
     7081 tggagacaga ttcaggccat tggtaaccaa aaatctcagg aaccgcagca atcaaaatga
     7141 aaatgcttga aaataaaata gctggcatcg ctgttaaaaa tccgtcacga acggctccta
     7201 aataactatt acgtgagatt ttttcaaaaa atggtttccc tttttcgatt tgtttaatga
     7261 ttccatccat agcgttcttc tcctatcctt gattctgtct gtacagtaca ataaagtctt
     7321 gaatcagatc tctcagcaat aaagtggtca ttaaatgatc ctgcccatgg ataaaaatga
     7381 atcccatctc catctctttc ccagatgctt cttccgctaa aatttttgtt tgcgaattat
     7441 gtgcaagggt taaattttcg tcggcttctt tcaacgccga atcaacatgt tcaaagtttc
     7501 cttgtcttac ttctttcaat aatttcaata atgtactgcg agcgtcgcct gaatatgcaa
     7561 cgatctcaaa accaagcatt tgtaattcct ctttattcat cttttcttcc tccattatca
     7621 cttattaaat aatcgttttg gtttcactga ccaacttgta ccagtaagct gattcttttg
     7681 ggtaacgctt ctgggtttca aaatcaacat aaaacaagcc ataccttttg ttatacccat
     7741 ttgtccatga gaaaagatcc ataagcgacc acaaaaaata ccccttcaca tttaccccgg
     7801 ctgttatcgc cttgcttaag gattccaaat agactctcaa atagtcgatt ctcggctgat
     7861 ccataataat gccatcttca aactgatctt tataccccat cccgttctca gtgatataga
     7921 ttttgttgta atgtgggtaa tcgcttttaa ttcgcagcaa caagtcatat agaccttctg
     7981 gataaatcag ccagtcccaa tcagttcgag gaattccttc tttataaata cgttctccga
     8041 ttcccttcac tttataaaca gaggtccctt tttcacccgt accgttatga tggattgcat
     8101 tttctccatc gtaagctttg acaaaatggc attggtagtg attgatccct aagtagtcat
     8161 tacgcgtgga tgcttttttc aattcaacga aatcttcttc aggaaaatga tagctggcct
     8221 gatttgcttc acatatctca tcaagagcag tcaaggtttc agttgaataa tagcctaaat
     8281 aggtagcatc taataagaaa cggatcgaca atgcatcgtc taaaaaggca gcatgcttat
     8341 cttccggcgc gtctgttgcc gcatatttcg tctctaatga gtgaactaca ccaatttcac
     8401 ccggaagttc gttttctttg aaatagttca ctacacgggc atgagcaacc atcatattat
     8461 gaagacaggc gacgatcttt gtaaaatcat atttgattcc tggtgggaaa acacctaaca
     8521 aatattgatt ggttgcaact ggatagattt cattaaaggt actccacacc ttgacttctt
     8581 tgaattcatg aaaacaaaaa atggcatagg aaacaaatgc ttctattgtt tctcgattca
     8641 aaaaatctcc atggtcaaac aaacgtttag gggtatcaaa atgatgcaac gtcacaaacg
     8701 gcgttacgtt tcttttctta cattcttcga acacacgatg ataaaaagcg attccttctg
     8761 gatttggttt cccagctcca tcaggaaaaa tgcggctcca agcaatggat aatcttagac
     8821 catttacacc aaaacgttca cacaattcaa tgtcctttgc atattgatga taaaaatcac
     8881 ttgccggatc gggactgaat cgcccttgtt cctctaaaaa gtcatcccaa gcaacagcgc
     8941 ctttgccacc ttccttagtg gctccttcaa cctgataggc tgctgtggct ccgccaaaaa
     9001 taaaatcttc tggtaattta agcatgtaca tctctcctca aatctataat cagttttcgc
     9061 tctttctaac tgattgcatt gtaaacgcat ccaaaacatt tgtcaacaaa aacaaacaca
     9121 aataatcata aaaaccagaa aacagcaata aaatctattg taacaaacat aaaaaccacc
     9181 ctaaatgttt acttttgttc gaattagatt gactgttgtc cgtttatatg atagattatc
     9241 cttaaattag aggtgaagac aatgttgaaa aaagaaagat tgcttactat cgtagaaatg
     9301 gtcaataaaa aaggaatcct taccgttaat gaaatcatca atcaattaga tgtttctgat
     9361 atgaccgttc gcagagattt agatgaatta gaaaaatcgg gcaagctgct gcgtgtccat
     9421 ggaggggctc aaagtctttc ctacacttta gatcaagaac tttctcacac tgaaaaagcc
     9481 actctgcaga ttcaagaaaa aaaagaaatt gctgccacag cagcctcctt gattcacgag
     9541 ggagaaacga ttttcttagg accaggtaca acaatcgaac tattggcgac gcatttactg
     9601 aacaagaaaa ttcggatcat tacaaataat tatccagttt ttgatattct tgctcaagca
     9661 gatggaccag aaattctact gattggcggt gatttccgga aaaataccgg cgcatttgtc
     9721 ggaccgatca ccaacgacaa tttaagaaga ctaaagttta caaaagcatt tatcagttca
     9781 aatggcgttc acaacgaagc gatctctact tacagtaccg aagaaggaga agcacagcag
     9841 atcgctctaa ataattcccg taagaaatat ctactcgttg ataataaaaa attcaatcga
     9901 gatgattttt atgtgtttta taatttgcat gatttcgatc tgctcattac ggatcgccag
     9961 atctcgcggg aggttcaaga gcactatgca caatatgtcg atctgcaaat tgctgattca
    10021 accgaaaata ccactatagg agcgtaacta tgaaagggat tatatttgat ttcaacggaa
    10081 caatgttttt ggactctcat ctgcatgaag ctgcttggct gcatatgata catgatcata
    10141 caaaagatgg tttatcagac gaggatattt taaaaaatat tcatggtcgg acgaatacgg
    10201 agattttgaa acatttcatt tcagagcatt tgaccgcctc tgagatcgcc gtattgtcag
    10261 aagaaaaaga aagctattat cgtatgcttt gcttgcaaaa tgaaaacgag cttcagttga
    10321 caaaagggct tgaagcgact cttgaccagc tgactgacca acagattcca ttaacgattg
    10381 ccacggctac tgtcaaagag aatgttgctt tttattttga tattttcgat ttagcccgct
    10441 ggtttgactg ggacaagatt gtctatgacg acggctcttt ccctgggaaa ccgcaaccgg
    10501 atatttttct taaagcggca gacaaactga gcttagctcc ggctgattgt cttgttatcg
    10561 aagatgctta ctcaggatta cttgctgcca aacgtgcggg gatcggaaca attattgcaa
    10621 ttgatccatt cggtaaaaac aaaacagtat ttgaacaggc acagctaaat aaagatggaa
    10681 ccatcaaaga ttttactggt ttttggacag agcacctgtc tgccacatcc gttaattctt
    10741 aaacgattca actaactacc aaagaaaagc aactgaaatg cacacctcct tttataaaaa
    10801 actgccagtg aaacgacatg aaagatacac tgctatatcc ttagtgaaat atagcagtgt
    10861 atctttgact ttttcattct tttaaccata tgacgcgttc atctctatct ataattaaca
    10921 ttaaaaatag tgttttcaca ttatttctca aaccaatctt aaaaatcatc aggagtactt
    10981 ttttgatata cttttattaa tgctgggcgt aatggttaaa agatttccac tagttcctct
    11041 ccaccaagct gagttgaaaa atgcttatct gtaaacaaca tagacaaaga ggaaataaac
    11101 gtttccgtat ctttctctgt cccaaggata ttaattaatt ttcctactag gtaagtatca
    11161 ttaaacttaa gatctgcctt cacatcaaaa gttttgataa aagcttttat catgcgtaca
    11221 attttgtctt tatcgccata attgatttca ccactcatat caatatagtc actaatccct
    11281 gaagaaataa tggcaactgc ttcttctttc gccttatcag ataattcatt taatgactgg
    11341 gattgattga tgacttcttg agttttatct gttacgtaat ctctcattat cctatcggca
    11401 tactccgcaa agtaatcttg aaaaaattct tctacagggt aaccaacttc gttttcccaa
    11461 ccaggagaaa tttcattatc agtacttatt aaatctatta aaaaccaacc cgttctctga
    11521 gtgttgattc tataatattt t
//
LOCUS       DAZAFF010000085        11539 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000085 DAZAFF010000000
VERSION     DAZAFF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.086"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            343..549
                     /locus_tag="ACG9BB_002240"
     CDS             343..549
                     /locus_tag="ACG9BB_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFN2838167.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(751..1752)
                     /locus_tag="ACG9BB_002241"
     CDS             complement(751..1752)
                     /locus_tag="ACG9BB_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN2838168.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1757..3670)
                     /locus_tag="ACG9BB_002242"
     CDS             complement(1757..3670)
                     /locus_tag="ACG9BB_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN2838169.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3838..4344)
                     /locus_tag="ACG9BB_002243"
     CDS             complement(3838..4344)
                     /locus_tag="ACG9BB_002243"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFN2838170.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4504..4944
                     /locus_tag="ACG9BB_002244"
     CDS             4504..4944
                     /locus_tag="ACG9BB_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN2838171.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4970..5998)
                     /locus_tag="ACG9BB_002245"
     CDS             complement(4970..5998)
                     /locus_tag="ACG9BB_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838172.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(6053..6127)
                     /locus_tag="ACG9BB_002246"
                     /pseudo
     CDS             complement(6053..6127)
                     /locus_tag="ACG9BB_002246"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6130..6486)
                     /locus_tag="ACG9BB_002247"
     CDS             complement(6130..6486)
                     /locus_tag="ACG9BB_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838173.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6784..7758)
                     /gene="sgrA"
                     /locus_tag="ACG9BB_002248"
     CDS             complement(6784..7758)
                     /gene="sgrA"
                     /locus_tag="ACG9BB_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFN2838174.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7954..8793)
                     /locus_tag="ACG9BB_002249"
     CDS             complement(7954..8793)
                     /locus_tag="ACG9BB_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFN2838175.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8978..9865
                     /locus_tag="ACG9BB_002250"
     CDS             8978..9865
                     /locus_tag="ACG9BB_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFN2838176.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10459..11153)
                     /locus_tag="ACG9BB_002251"
                     /pseudo
     CDS             complement(10459..11153)
                     /locus_tag="ACG9BB_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(11137..11535)
                     /locus_tag="ACG9BB_002252"
     CDS             complement(11137..11535)
                     /locus_tag="ACG9BB_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFN2838177.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3437 a         2299 c         1730 g         4073 t
ORIGIN      
        1 ggcttttggc ctcattttag gttatcattt gtaattttat ttcacagttg atatgattgt
       61 tatgtaaaat aaaaaagttt aggatacatc gaatttttgg ggaaattctt ttgggggtat
      121 cctaaatctt ttctattata ttcccatatt ttctattatt ggtctagacg tatgatggta
      181 aactttgtac ctatctgatt gctgaatcat taattgctta tcattagcca tctttgtttc
      241 aagttcgcta gatgatcatt ttattcacat cctaacaaac ttgtcacatt tatagattct
      301 taatctattt atcatacaat tataaaaaag gaggtgctgc ttatgaaagc tatccatatt
      361 tctttgcttt ttttcgctag tgcattgatt ttatttgcta ttcatctttg ttcccagcct
      421 tatatcaata acaacggaat attagtggaa ccagcattct tttgcttacc actcggcttt
      481 ttgtcccttc ttgcctccgc aggcttcgga tttgttgctt tttttaaacg atcaaaacaa
      541 caaatctgac ttataaaatt tacgtaaaag agatcgtgac aataatcgtg tgatattccc
      601 aaatttcgaa taaaaggtgc cccttgccat ctctaactcc tcagtcacaa gttacaatat
      661 tcataaacca tgaaaagctt gtttattgaa attgctcctt gtgacttgga gctaagtgac
      721 agattacaac ctcttttgca tcataagact ttactccttg aaaaacatta ctaacttttg
      781 atatgtttct tgcgcttgtt tattgctgaa atcaaactga tattcatggt tcaaaacagc
      841 atttttctcc gcataatcta cttttgtgac tttcaccccc gctttctcaa atacagaagt
      901 tacttcttcc atttgtttcg taaacgtatt tgtatttcca tccgttaaga atgtgttttg
      961 cgtccaacga tccgcatgtc tgaccaaggc taaacttttg accttctctg attgttcaaa
     1021 gtccttatta tcaaagtaag cccatccaca tttttcataa agaaagctag agattttatc
     1081 acttacttga gtaagtgcag aaggatccaa cagaccagac aaagaaataa acttcgtaat
     1141 ctgcttgttc tccaaagtcg gatcaagatg atcagctttt ttaatttctt cattttgaag
     1201 tgctaacaaa tattctccac taatttgtgc acctgccgaa tttccaccaa tcgaaatatt
     1261 cgaccaatct gcttgatctt ttatctcttt tttagctgct tgaatggctt cgttcaattg
     1321 gataactggt gtaggatgtt ttgcttccgg tgatcgttca taatctatat ttacgatgat
     1381 ttgctgcgtg tctgaacata gatattccaa ataatcacga caatcatttt tatcccctcc
     1441 tacatatgcg cctccatgca tccagaaaag aatcggtaat tttttctttt ccactttcgg
     1501 tgtgtagata tccatattat tatttttgta ctttgaagga taggatattt ctttttttat
     1561 atcgatttgt tcgatttttt gctggaaatc atctggtcgg gcatatactt gttctttttc
     1621 acttgtgtca aattgttttc taacaagcaa gctaatcggc tttggtgtga tttgattaat
     1681 caaaaataca gtgactaaaa taactacgat gataactggt actgcaatca ctattcgctt
     1741 gaaccatttc attcatttac cctctctttc ttcgccaaat ccatgtgata atcgtgctaa
     1801 cagataaaaa tccacctgaa taaatgagaa gcgaatgttt cttttctcca gttttcggca
     1861 acattctttt ttttgcatct gtcttttttt gtactttcac tgtcatctga atcggttgcg
     1921 tatcatcact tgattgatcc tctcgaggtt cattctttcc cttattgtct gcattctctg
     1981 tcggatcttg cttttttggt ggagctactg ggattttttt gtaatagaaa ataatagttg
     2041 gtatcgtctc attaaaaatc ccttcagtcg gtccttcaac atttgctaat tcgtatcctt
     2101 cgaatgtctt ttgctctgtc atataatttt ctccataagc tccgtaaagc tggatttcgt
     2161 cctggtcttt gagtagactt ttattgtttt catccacata ctttacgttt atctgatcat
     2221 ggaacactac tttcaaaaag tccgttacat gtttcgtgta ggcagcatgg tcaacttcct
     2281 gtgcctttcc atgccctgct ccttctacta ataaaagatc tttataccct cctgataact
     2341 catataattt ttgcgcgtta gacacaggta ctacatcatc tgctgttcca tggataatca
     2401 atttaggcaa tgaatctgac attccaatcg taggtagagc agcttttgta tccaaatgca
     2461 tctcctgatc aaaatagtat tgtttcaaaa ggcccataaa taaatcttca tgctcttgat
     2521 ccccaatgat gtctaactga ttttttatgg catccggtag tgcgggggct actgtctggc
     2581 tgtacgtaaa acgtagttgt tcatcaattg atgcaaatcc gcaattttca ataattcctt
     2641 tgacattgac aggaatatct ttcgctaata cagacatcat cgtagctgct cccatcgaac
     2701 caccataaag taaaatcttt tgagatggcc gctctcgaac ttcttgattt atccagtaaa
     2761 ggacgtcatc tgactcatat tgtccatagg tcacataatc gcctccgcta ttaccagtcg
     2821 ctctggaatc aacaaataat aaattatacc cggccttata aaaatcattg tattcttctg
     2881 taacgatccc gttattccaa ttccctcgaa atccgccatg gatcatgact gttttatctg
     2941 aaccttgatc gacataatat gcatatacat ccatcatttt gccatcttgt ttgtttagta
     3001 cagtttgatg aagttgtttt gtatttggat agaagcctgt gttttctgta ggcgttgtcc
     3061 catcatctgt acctccaagc atcgacatca acacactgtt ttttgcaaaa gcgatatcgt
     3121 acaatagata agtagcaatc agatgtttga taatctcttt atctacacca taatcattaa
     3181 cagcaagctc agtgactaat aactctatta attttgactt gatgtcttct gttggtccat
     3241 taagtatttc tgtcatctgt ttaccgaagt aattcaaata ttctttacca ttgaaatttt
     3301 cacctttacc gatatcatca ttttctgtcc cattctcttt gtcacttact ttagcagaag
     3361 aggattcgtc gtttgacatt tttgtagctg ttgcttcttc tgtttgttgg ttatctgtgg
     3421 tagcggtcgt ttcttgatct gtgctagtaa cagatgtctc aaccgttgtt tcagtatttg
     3481 tttctgtttg tgaagtactc gatttcgttt catttccagc atcagtagtt tctgtggttt
     3541 ctacctcccg agattccgat tcggaagaaa tgctctctcc atatactgtc cattgcggga
     3601 cgcttgtact tacagcaaat aagatgacca acgctccctt taatacttgt tttgttctca
     3661 agctattcac tcctttttat gtttctagga gaattatatg taaacgcaaa cattttttat
     3721 acaaaaaata aatggtttat cccataaaaa tagaacagga ttattttctc attttacgta
     3781 ataaagcaga atggataaac cagtatccat tctgcttttt ttaaacgaag aaacaactta
     3841 ttgctccaaa aatgtcagaa atcgtttatc ccaaattttc tcgtaaaaag cgattgtttc
     3901 ctttgctttc ataaaaggat tatcataagt tgtcgttgct cctctacaca tcataagctg
     3961 gtatcctaat ccatgagcaa acttcacagt cgtatctata caatactctg cttgtgcccc
     4021 acataattcg atcgaatgac agtccaattc agttaataaa acactcaaat tagtcttata
     4081 aaatgaattc gcatgggttt ttctcacaaa ataactgtgc tcaggaatag ataattccga
     4141 caatagctcc catggttttg tttgtggcac taattgagca tcttcgtgtt gaacgaaaat
     4201 aactggtttc ccaatcgttt cgtatgtttt gattctttta ttcactaatg ctgtcactgt
     4261 ttctaattcg tgtatcggcg aagaatcaaa acatacccct ttttgtaaat cgatcactac
     4321 tattgcgtcg gctgtgtttg acatacttgt gccttctttc ttttttcttg atttaatagt
     4381 atttcttatc tagctagttc cattatacgt gacaacattt tcttatttta cacctctaaa
     4441 tggcactttt cgactatttc ttcatgttat tatttcataa atacttagct attggaggaa
     4501 tgtatgatga ttaaattgta tacaggttct ggctgctcgt cttcaagacg cgcaaaacgt
     4561 tggcttatcg tccatgatat accgtttatc gaacagaact taattcgtca accactaacc
     4621 aaaaatgaat ttatccactt actaaaattg acaaacaacg gcgtgaccga aatgctctct
     4681 actcattcta ttagttataa aaatcttgtt cacagaatag atgaactcag tttgagtgaa
     4741 ctatatattg agattcgtac atccccttct ttattgaaaa aaccgattat ctacgaccaa
     4801 tctgcaaaac ttttgactgg atttagcgaa gataaaatca ggatgttcct ttctaaaact
     4861 aaaagaaagc aagagctcaa cgaactcttg ctttctggtc atcttgtcga ttttaaagaa
     4921 aataatcttt ttgcaatgtc ttaattttgg cataaacaat ctgcttttct taattaacga
     4981 aatccaattt ttccaatatg gatctatttt tctcattcct gatttttatg atttcagctt
     5041 tcagacgcgc ccaatcattt tttgtaggtt gtccattcca gtaaaatatc tgtgtattcg
     5101 tttgatcata tgcctttttg tcgataggat aatcgctaat gacaatatca ggtaactgat
     5161 cagcggatgc actatatttt attgggcgtt cagtcataga taatatcctt ttctttgccc
     5221 ataattcctg tgttttacca tactttgatt gaatatagaa tatgacaggt tgttcgtgtt
     5281 ttataaaaat atccctgact aacatacagt attgaaaaat caaccgtctg ttcttttcat
     5341 aataagtatt aagctctgga attctgcgag ctaccccact aaaaaactgt tgcatgattg
     5401 gatacatcac tggaaatata tgctgaaaat ctctgtcttc tgctgatttt ccaaaagcat
     5461 ccaccttctt ttttgtccca aaaacccttg aatacaaatg gatcattgcc aaattgacat
     5521 atagataatt tttttctgaa gttgtaaaac aaatcttgaa gtttttttca aaattttcta
     5581 caatagacga ttcaatccag ccccaacttt cttgtacttt atctggagta tccaacaaat
     5641 cttcttccaa ataggtgttc attacaccaa acataaaata aaggtaagct tgctctgttg
     5701 gagtgatgtg attcatcgac cagaagtttg cttcaaaaaa aggctctagt atgtaatgcg
     5761 taaactcttc ttttgttatc aatggatgca caaatttgtt catctcttct gggacatacg
     5821 taatcgagtg gccttgtttc attcgttgca acgaaattct tagtaggctg atccatttct
     5881 ttctatctat actacgcgta tatggcgcat gtttcgcaaa tttttctaat agttgacgtt
     5941 tattttttat acttgataat atatcagaat gattttcgtc gaatgactgc caatacatca
     6001 agtgaaaaaa gtatcggata cggtattctt ctccttctat cggattacct tttttcaagc
     6061 tgcaagataa atgatagttt ttcaaaatag tattaagttc tcttaatttt ctttgaatcg
     6121 tacgcaaact tatataattt tttgactaag attttctaag gattgataag tacctttgta
     6181 aagtgaatcc agtaaaatat aaccaatcgt tccctctaaa agtatagaat aaactttact
     6241 cagtaaaaac tgagaagaaa actcgacttt tatcatcttg ttttctgaaa gaaaagataa
     6301 tttatattct ggataccttt cgcaaaacga ttctgcttgt tgaatcaccg aattcatctt
     6361 agccagggtg tagtttagat ctaaagcgat atccttcacc acatataatt tctcgtctct
     6421 ttcataacaa tagttcaaaa attgatatac ttgcctttca ttgatatcaa taaattcttc
     6481 aaacaaagta catactcctt tttatctatt atttagactt attccgacag ctacatgaat
     6541 aacgtacaaa tagattcgtt actgattttt cagagatact accatgcgca gtgtgcattg
     6601 tcaattattc ttgctcttat tttccatttg tgttcctgtt atttcctgtt tctcaaatat
     6661 gttagtaaac aaaaatagat ctggcaaagc tacaacttcg ccaggcaata cagcactctc
     6721 gaatgaaata ttcactcaaa aatctatcgt tttattcaat tttagatctg ttgcttttat
     6781 tctttatttc actcgatgga agagagaacg aaaatagctt gctcctacaa tcaaaatgag
     6841 taacacaccc atccatgtaa ttagcttgtt caaggttcta ctaccagttt ctggtaaatc
     6901 gctttttcta ttatctgttt ctttcgcttt ttcttgatga tcaattccac gagaaacaaa
     6961 atcaggctgt tttagcacac tttttaggtt gctccctgca tttctctttt gttccttagt
     7021 tggtatgata ggtaccaaat caagttcatt atttactttt cctgtgctgc tttcgctgct
     7081 cgtgcttcct gtgctactct cactactcat gcttcctgtg ctactttcac tactcgtact
     7141 ctccgtactg ctttcgctgc tcgtgcttcc tgtgctactt tcactactcg tactctccgt
     7201 actgctttcg ctgctcgtgt tttctgtgct aatttcactg ctcgtactct ctgtactgct
     7261 ttcaccgctc gtgttttctg tactgctttc actacttgtg ctatccgtac tgcttggcgg
     7321 tccaacaact gtccaactgc ttggtttcaa attctccgtg atcgtcatct gtccatcata
     7381 aaaaacaatc aaatcaactt tatctttact cttgatctca tcaatcgata gttctgcaga
     7441 aatcattatt gtatactcaa taccagcggt ccattgtacg tcttttaaat aaacttcccc
     7501 aataactttt tctgaacttc cattaccatc ccaaccatat gttagaacag catgtacatc
     7561 actagcattc acgcttttgt ttggtttgac tggaatagta atgacttgtt gtgtcatgtc
     7621 ataaacaatc cgatcagttt tcacagtaaa tgaaagattc tcatttgccc gttcatttgc
     7681 agaagtactg attccaatca ttgctgcaat tatactcagc ccaagaataa caaataatac
     7741 ggtcgctgtt ttcttcattt ctatcactct ccatataatt accttctatt tgataaagga
     7801 attatataaa atgaaaatga tgagaacacg acagtcagtt gtgaaaatct aacaaaagat
     7861 gtcgtgtatt cgcttgaatt tgtttacttt ttcgtttaat caatgacaga cttaaaaaag
     7921 caagaccgat atcagccttg ctttcttcat cttttattga taaacagcag tgtatttctt
     7981 catccgcttt gttttatatg catagagtaa caaaccaaca atcaagacta acaagaataa
     8041 tgtagttgat atagatgctt cagccccaaa gtttccccca gtaattattg tattttgcga
     8101 ttggatcaga gtggagaaaa aaccttgttc ttgcgttccg ctgactggta tacccaaaat
     8161 agctccttgt gtccaattcc aagtaatatg agcaccaata gccgtaaata aacttcctgt
     8221 actgtagata agtgcgctga aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa
     8281 tgtcatgtta ggattcaatc cgtgcaaaag agcaaataat gcagcagatc caatcattgc
     8341 tattaacgga ccaaatctaa gcatctgatg ctgaaaaaag ccacggaata aaagctcttc
     8401 tgatgccccc actgcaataa ataaaataaa actcgttatc agcggcacaa tgtttgcgtt
     8461 tcctgaatct aaccgatacg cccccgtcaa atataatgga ctagtaataa gcagaaacac
     8521 tccaattcca acaacgagac ctcccgctaa atacccccag tcttttcctc gaacagaaac
     8581 accataatct tctattcttt ttccttcgac cttactagca agaaataaag ctagtaaaaa
     8641 aacagctaaa tgtccgaccg taccggcaat tgctccaatc cattcactat ttccaataaa
     8701 catatccgca agtgcattat tgacaagaat tcctaaaaca aaaccaacaa agactattaa
     8761 aacagatcca atcaaccgaa taatcctgat catctttcat ccctccttta tattaagttt
     8821 aacaaccctt ttctttcttg tctcgtgaaa actatgtaaa tgttctccat catagagaga
     8881 atagattttt acgaactaag aatatctaca gcaaccttac gaaaatgaaa gactgtctcc
     8941 ctaattcttc tgttagactg tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca
     9001 actactaatt atgtgccttt cagcatgtgc aattttacta ttctcaggtt gtagcaagtc
     9061 caatgaaacg attgcaacgt ttaaaggtgg aacacttaca gttcaggatt tttatgataa
     9121 cccaaaagtt aaatcaaata atcaaacact attaaaaaaa atgattgtct tcaaagcatt
     9181 tgatgatgta tacggaaaag agatttcttc aaaggaagta acaaaagaat ataatgaaca
     9241 aatcaaaaaa ctcggtccaa actacaagga acaattaaaa gcagttggcc aaacagaaga
     9301 gacatataag aaactcttta aacagatgct ggctttccaa tacggtttga aagccaatgt
     9361 aaaattaaca gataaagatc tagacactgc ttggaaagaa ttctatccag aagttagtac
     9421 acagatcatt ctattttcga ctaaagaaga agcagataaa gcaaaaaaag aagcaaatga
     9481 aggagaaaat ttctccaagc ttgttcaagc ttatggaaaa aataaaacat taaaggaaac
     9541 tgatggaaaa atgaattttg attcaaccaa tccagagatt cctactgaag taaaaaaagc
     9601 agcatttaaa ctaaaaaacg gggaagtttc tgatatcatt cctgtaacag atccaacgac
     9661 ttatcagcaa agctattatc tagtaaaaat ggtaaaaaaa caggataaag gttctaataa
     9721 agacaaatac aaatctgagc tagaaaaaat tgctaccgaa gcaaaaactc ttgatacgga
     9781 attcatggat aaaacaatcc gtaaagtgat gaaaaaagac aatgtaacta ttaaagatcc
     9841 ttatgtaaaa aatatattca aataaacaaa tttgacccaa ttggggatgt ggcaaaattt
     9901 gagaagtgat ttttggaaat tccttcttat ttttgaggca aaccactttt gccacaactt
     9961 catcggggcc tttttttatt tgtaccaaaa caaatcactg gatttgttac aatcatgagg
    10021 atataattta aaaaagtttt ttatttctat cacatgaatc aatgcgccat taagtagatt
    10081 ctttcatgct tccctatttt ttcacttgcc gtatcgcttc aaaaacaact atcataaact
    10141 caaactcgac tgaaaagtta ttttaaaaaa gactaaaaat atcccttctg gacttgttct
    10201 tcttattctg ttatactaga gaaacacgaa agatattttt atgcgacact tttcatttgt
    10261 ttttatgaaa tttattcaaa actggcgtaa cttcagagtt acaaggatgt attagaggtt
    10321 agggaataca tcgtttaaat gagaaataag taatagttag taatagtcac ccaaacttta
    10381 aattaatgaa tattgaatcg tcagatagcc tcctcgagtt ttcgagaagg ctatcgttta
    10441 ttttatggag aaattgattc agaagtaatc agcgaccttt tttagttcac caatcgtttc
    10501 tgcttggaaa gtttgcaccc aatcttcttg gaagcccctc actgctttat caatcgaggg
    10561 catctctaat acttgatcta gtagctcttt acctaacgtt atcgctgagc tccagatcga
    10621 atggctgtag tgacttgttc acaatttttg aaactagctg ctaatatttt tgttggtctt
    10681 tgcgtacgtt cgatctcagt ccgcaacgaa tcaatcactt tcatagcatc aatgccttga
    10741 ttttgcatcc tgttataata aggtgcaata taatctgctc cattattaat agctaaatac
    10801 ccttgtaatt ctgtgtaaat agctgtaccg gtaatcaaat aaccttcttt tttcaacttt
    10861 ttgatcgctt gaaggcctgc ttgattcacc ggcaccttga taaacgtatc ttttcccagt
    10921 tcttctatga taactcgagc atcctctatc atttcttctt ccgttcttcc tattgtctga
    10981 atatggatag gaatctgtcc gattattttt ttgatttctt tcatatgcga gaagaagttt
    11041 gacgctcctt cttttttcag aattgtagga tttgttgtta cgcctgttaa attcaaaaac
    11101 tgttgacaag aataaatact ttttgtattt gctgtatcaa gcatgatttc catatagctt
    11161 tcccccattc tgatcctctt gatcgatttt aatgattgtc ccctttctta aatgcggcat
    11221 ttccttatct tctacataca ttgttcctgg aagttccggc tcatgagatc catcaaattt
    11281 tatcgtgata tgtcctaatt cattcaaatt agtttggaca acatctccta ctgctgtgat
    11341 tacatacatt tgtttatcaa aacagagctt catatcacaa gttatttctt ttcttacctg
    11401 attcaattca atcttataac aataatctgt taatgtttcc ggtgcttcat ctccaaataa
    11461 aatcattatt ttttctttcc tgaatacttc tgcttctata ccaacatctg ttacaaccgt
    11521 ttcaaagatc gtcatctct
//
LOCUS       DAZAFF010000086        11539 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000086 DAZAFF010000000
VERSION     DAZAFF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.087"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..736)
                     /locus_tag="ACG9BB_002253"
     CDS             complement(251..736)
                     /locus_tag="ACG9BB_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFN2838178.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    826..1171
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1216..1857
                     /locus_tag="ACG9BB_002254"
     CDS             1216..1857
                     /locus_tag="ACG9BB_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFN2838179.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1876..2511
                     /locus_tag="ACG9BB_002255"
     CDS             1876..2511
                     /locus_tag="ACG9BB_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFN2838180.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2529..3344
                     /locus_tag="ACG9BB_002256"
     CDS             2529..3344
                     /locus_tag="ACG9BB_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN2838181.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3663..5099
                     /locus_tag="ACG9BB_002257"
     CDS             3663..5099
                     /locus_tag="ACG9BB_002257"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN2838182.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5288..7720
                     /locus_tag="ACG9BB_002258"
     CDS             5288..7720
                     /locus_tag="ACG9BB_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFN2838183.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            7950..9017
                     /gene="rlmN"
                     /locus_tag="ACG9BB_002259"
     CDS             7950..9017
                     /gene="rlmN"
                     /locus_tag="ACG9BB_002259"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFN2838184.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRSEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            9007..9213
                     /locus_tag="ACG9BB_002260"
     CDS             9007..9213
                     /locus_tag="ACG9BB_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838185.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9210..10313)
                     /locus_tag="ACG9BB_002261"
     CDS             complement(9210..10313)
                     /locus_tag="ACG9BB_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN2838186.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10865..11413
                     /gene="aac(6')"
                     /locus_tag="ACG9BB_002262"
     CDS             10865..11413
                     /gene="aac(6')"
                     /locus_tag="ACG9BB_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN2838187.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3836 a         1947 c         2483 g         3273 t
ORIGIN      
        1 taaatatgac tgaaatcaaa agaataaaat tccgatttat aatcgaaaaa agaagcattt
       61 ttatatatgc ttcttttttc gtagaatcaa taaatatgga tttggctaac tatggaagaa
      121 tactcgtatt agttttcagc gttcatacta gttgttacat tgtttgattg gccaaaagaa
      181 tttctgatat attttgtgct aaataaagaa aaagacgctt gaattgggaa ataagaaaac
      241 tactctatca ttacaaattt tcataaactg gcagatgttc taagtaaaca agtatctttt
      301 caaagtcgga aaaatccata atcaacgtag tggtattgat atttggatga aagcctacgg
      361 tatcttgacg atcaatagca gcatcgatca cgacttggat ttttttctct ttgtcgtgca
      421 tcaatgctaa tggagtcact gtacctgctg gaacgcctag gaggtcagga agctgttctt
      481 cagaaacaaa agataagcgc ttttctttta attgttcagc tagttgtttt aaatcggcgt
      541 gcttttcatc aggcaagatg acaagataga tttgttttcc ttttcttgat ttcaataata
      601 gattttttac ttgcggacca ggtagggaaa aagggacatt tttgactgac gtaatcgcag
      661 gatgatcgac tttttgaaaa gagataccca gtttttttag tagttcataa gcttcttgtt
      721 cggttgcacg actcatcgcg catcaccctc ctttttttat tagtgtatca taaaaaagag
      781 ttgacaaatg aaaaataatc atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa
      841 agaagagtag ccgttaggtg tttttagaga gtctctggtt ggtggaaaga ggcagcagtt
      901 aaccgtgaaa cacatcttgg agtcgatttt ctgaagcgta tagtaggaaa atccggggag
      961 cccgttacag ctgcggtcgt tgctagatga ccagataagg ctggcttttc cgtgaggagt
     1021 cggtaaaaaa aggtggaacc acgtgattaa cgtcctttga tcttcggatc aaaggacttt
     1081 ttgttttcat ctgaacggca acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca
     1141 gaggtggaac cacgtataat acgtcctctt ggcatttgcc aagaggattt ttttatttta
     1201 aaggaggaaa agaagatgga gtatttgatt gaagttatgc cgcaattatt agtaggagca
     1261 ctcacaacat tgaaattatt tttatttacc ttgattggct cattaccatt agggattgtt
     1321 ttagcattag gattgtcttt gcattttaag ccgctacgct acttgcttca aacatatgta
     1381 tgggttatgc ggggaacacc gctgttgttg caactgattt ttatttttta tgggttgccg
     1441 acgattggga tcgtttttga tcgtttcgaa gcagcactca ttgcctttat cttaaactat
     1501 gcagcttatt ttgcagagat ctttcgtgga gggatacaat cgattcctca aggtcaatat
     1561 gaagcagcgc aagttttgca attaagtccc tttcaaacgg tgacgaaaat cgtcatccca
     1621 caagtgacaa agattgtttt accttctgta gggaatgaag ttatcaactt ggtcaaagat
     1681 tcttccttga tctatatatt aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg
     1741 gaacgcgacg cgactttact gcctttgcta ggcgtggcaa tcatctatct tacattgact
     1801 ggtattctaa caatcgtaat gaaacaagtt gaaggaaaat taaattatta ccgctaactg
     1861 ataggaggaa gaaatatgat tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa
     1921 ataatcaaag atttgaatct aatcattcca gatgggaaaa cattagctat cgtaggtcct
     1981 tccggtggcg gtaaaaccac gcttttacgt atcttggctg gcttagaaag ttctgacagc
     2041 ggagaatttt atttagacgg tgtaccgttc aacccattcg agcttaaaat gaaagaacaa
     2101 gttgtaggag tcgtattcca agacttccag ctatttccac atctgaatat ttttgaaaac
     2161 attaccttag caccaaaaat ggtattgaaa cagacaaaag aagaatacac caaaaaagca
     2221 gaagaattag cggcttcatt agggattagt gatttattga atatctatcc ttatcagctt
     2281 tctggcggac aaaaacagcg ggtagcgatt gccagagcat tggctatgaa tccacgggta
     2341 ttggcttatg atgaaccaac aagtgcttta gatcctgaat tacgccagca agtcgagcgt
     2401 ttgatcctat cattgaaaga agatggggta acccaaatta ttgtcaccca tgatttagta
     2461 tttgcagaaa atgtcggtga tcaattacta aaagtttccc caattcatta agaaaggaga
     2521 agatagagat gaaaaaaacc agttttctat tcgctttgat cgcaggtctt cttctttttg
     2581 caggctgttc gaacaaaaaa acttcagcag ataattgggc gactatcaac gaagagaaaa
     2641 aaatcgttat aggtttagac gatacatttg ttcctatggg atttagagat aaagacggga
     2701 agctcacagg atttgatatt gatttagcca aagcagtctt tgaacaatat gggattacgg
     2761 tagactttca gcccattgac tggtcaatga aggaatttga actgaacaat ggaacgatcg
     2821 atttgatatg gaacggctat tccaaaacgc cggcaagaga aaagaaagtg caatttacaa
     2881 aaccttatat ggaaaacgat caagtgctga tcacacctaa agaatcagga atcacaaact
     2941 ttggacaaat gaataataag agattaggtg cgcaaaatgg gtcatctggt tatgatgtat
     3001 ttacgaaaca accagaagta ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg
     3061 atagttttaa tgaagcattg atcgatctga aaagcagacg ggtcgatggt ctgctgatgg
     3121 ataaagtata cgcagactat tatttgaaac aaagaaacga actagaagac ttcaacataa
     3181 caaaaggttc ttatgagagt gaggattttg cagttggtgc acgcaagtca gatagtacat
     3241 tagtgaaaaa aatcaatgaa gccctagatc aactagagaa aacaggtgaa ttccaaaaaa
     3301 tatccgataa atggtttggc gaagatgtta cgccgagaaa ttaacaaata ggggactgtg
     3361 acaaaagacg tgtcacagtc cctttttccg aataaacagt gttctcctgc caactccttg
     3421 tgactcggag ctgaatggca ggctcacaac ctcttggtaa acggtgttca aaagccagtt
     3481 tttcggtatt aagccaaatt tatgcaaaaa tgtagaaagc cattttcaca aaatttttct
     3541 tactactcga aactaagcga ctttttcaca acctccttta cataatttta ttttgttgtg
     3601 gaaggggttt cgtgttattc taaaagagga acgattgaag gttataggaa aggaagagat
     3661 gaatggaaca caaagaactt acctcatttc caaaagattt tctgtggggc tctgcttctg
     3721 cagcgtatca agtagaagga gcttggcaag aagatgggaa aggagaatca gtctgggatc
     3781 gatttgtacg gatacctgga aagacattta aaggaacgaa cggtgatttg gcagtagacc
     3841 actatcatcg ttataaagaa gatattgctt taatgaaaga acaaggtctg aaagcttacc
     3901 gcttctctgt agcttggaca cggatttttc ctaatggacg aggagaagtc aatcaagctg
     3961 ggctcgcttt ttatgagaga ttgatcgacg aattgattga aaacgatatt gaacctatgt
     4021 tgactttata tcattgggat cttccacaag cattgcaaga tgaatataat ggttgggaat
     4081 cacgtcagat cattgaagat ttcactcatt atgcagaaac attatttgag gcattccgcg
     4141 ggaaagtcca ttactggatt agtttgaatg aacaaaatat cttcacttca ttaggttatt
     4201 tgttagcagc acatccgcca ggtgtgacag acccgaaacg aatgtatgaa gttaaccata
     4261 ttgctaattt agcaaatgca tctgtcatca ataaatttca cgagttagaa atgcctggaa
     4321 aaatcggacc aagctttgcg tatacgccaa attatccaat tgacagtaat cctgaaaatg
     4381 ttctggctgc tgaaaatgca gaagacctga tggccaatta ttggatggat gtctatatgt
     4441 ggggaaaata tccgattgct gccatgaggt tcttagaaga aaaaggatgg gcaccaacga
     4501 ttgaagctgg cgatgcagaa ttgctagaat ctgctaaacc agattttctt gggatcaatt
     4561 actatcaaac ggcgacgaat gcattcaatc cattaaacgg agttggtgct ggaaaaatga
     4621 atacgacagg aaaaaaaagt tcatcagaag aaactggagt tcctggaatg tataaaaaag
     4681 ttgagaatcc atttgtcgaa cgaacaaatt gggactggga gattgaccca gaaggattaa
     4741 ggatcggact gcgtcgtatc acaagccgtt accgcatgcc ggtactgatt acggaaaatg
     4801 gcttaggaga gtatgacaag ttgacagaag ataaacaaat ccatgacgac tatcgaatca
     4861 attacttaca aagccacgtc aaagccatca aagaagcaat ttcggatgga gcagaagtcc
     4921 taggctattg cacctggtct tatacagatt tgcttagctg gctgaacggt tatcaaaagc
     4981 gatatggatt tgtatatgtt gatcaagacg aaacacaaga aggatctttg gaacgatata
     5041 agaaagacag ttattactgg taccaaaaac tgattgaaga aaatggagag aacctttagt
     5101 tcacatactt aaaacaagaa ggtacggctg gatatggccg tgccttttct tgataaaatc
     5161 atttagaaat taagaaaaac tgtaaaataa gctgaaaaat gaaagaaagc gtcagttctt
     5221 tcaaacaaaa ctttagttcc cttacttttt tttgtataat gaaacttgat gaaatgaggg
     5281 ataataaatg gcacaaaaga aacggcctac taaaaaaagg aaaaagacga aaaaaacgaa
     5341 aaagcaaaaa cagcagcagg aacaaatgct gacgattggt gttgggtttg ccttgatttt
     5401 atttgctgtc ttcggctttt taaaattagg ttttttagga atattgattg caaacggctt
     5461 tcggatcatt gcaggaaata cttaccaaat cctctgtctt ttattagccg tattaggatt
     5521 ttggattgtc attaagaata cagagttttc aattggtaaa aaccgacgct ggtttggcgg
     5581 catactcttt tatttcggta tcttattatt gttacatgcc catttatttg gcaaactcca
     5641 tactggggaa ccaaatatca tggggacgac atgggatttg ttagccagtg atataaaaca
     5701 aagtcaagta gacaataacg ttggtggtgg gatgattgga gcaatccttt atcattttac
     5761 ttacttcttg attgcacagc ctggaagtta tttagcagca gtgctattac tggcaggagg
     5821 agcattcctg ttcagtaatc tggaaggcta tcagctgtta aatggtatcc aatcaattgg
     5881 agaacgagta caggaattat tggaaggtga tcctgccaag caagctcgaa agcaagcggc
     5941 aaaggaagag agaatgaaac agcgcgcaga agcgaaagaa gcaaggcgct tagctgcaaa
     6001 agaagctgca gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga
     6061 tgaatggcag gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag
     6121 ttttcaagaa aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca
     6181 gaccaataca gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga
     6241 atttgagatt gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct
     6301 tgattctata ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg
     6361 cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt
     6421 aggaccatcc gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat
     6481 tgtgaactta acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc
     6541 accgattcca ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt
     6601 ttcctttaga gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc
     6661 tttaggacgt gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt
     6721 actcgtggct ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag
     6781 tatattaatg cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt
     6841 tgaattgaat atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag
     6901 aaaagcagcg caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt
     6961 cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt
     7021 ggaaaacgga acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc
     7081 tgatttgatg atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat
     7141 ggctcgggca gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat
     7201 cacaggaatc atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac
     7261 agattctcgg acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat
     7321 gctattcttg ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga
     7381 ccacgaagta gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga
     7441 gaaaatgatg ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga
     7501 gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct
     7561 ccaaagaaga tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc
     7621 tcatggagtt gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga
     7681 agaaacatca gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt
     7741 gaaaaacgaa taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt
     7801 tttccatctt ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt
     7861 tctattcgat ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc
     7921 gaaactttga gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga
     7981 atgaagaatt aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag
     8041 tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga
     8101 aacaattgat cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg
     8161 tccaagaagc ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga
     8221 ttgagactgt actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag
     8281 gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga
     8341 ctgctggaga aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc
     8401 ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg
     8461 acaatgtaat ggactttttg catatcatca atgatgccaa aggattggca attggtgctc
     8521 ggcacattac agtttccact agtggattag cacacaaaat caaggaattc gctaataacg
     8581 gcttgcaagt caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga
     8641 tcatgcggat caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt
     8701 tagagaaaac aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata
     8761 gatcagagca tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg
     8821 tcaacttgat tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag
     8881 ctgatgtatt gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta
     8941 aagaacatgg tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa
     9001 aaacagttgc caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt
     9061 ttcttaatta aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata
     9121 aagctttttt tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca
     9181 gcctgcttga tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt
     9241 tcttgtgtag catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg
     9301 atttcataag gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct
     9361 aaatcggtcg tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc
     9421 ataaacacat agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt
     9481 acaccataac tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta
     9541 ccgtaataga atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc
     9601 aatccggtag taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg
     9661 tcggcactat ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt
     9721 ccgatttgga ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta
     9781 gattcgactt ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct
     9841 tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt
     9901 ccgtccatta cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc
     9961 gaaccaatag tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca
    10021 gtatcgcaag cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca
    10081 tgctggatca actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg
    10141 acaacctcta tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca
    10201 gtcttccctc caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt
    10261 tcatacgcat ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt
    10321 tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct
    10381 gtagatggag tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac
    10441 cttgaaatca tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa
    10501 taatgaattt ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc
    10561 taaaaattct aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat
    10621 tggttttttt taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc
    10681 caattgatgg ctttcctgat ctttcctatt ttgaataaag tgtttctgta taatttttta
    10741 ttttataagt aagataaata atgaaagaat agagatactg attggtagct aaagattttt
    10801 gagcgagaac atttcgtttt ttcaaaagga ctgttagaat aagcaagaac aagggggaag
    10861 acgtatgata atcagtgaat ttgaccgtaa taatccagta ttgaaagatc agctttctga
    10921 tttactgaga ctgacttggc cggaagaata tggagacagc tcggcagaag aagtagaaga
    10981 aatgatgaat ccagaacgaa tcgcggtagc agcggtagac caagatgagt tagtaggatt
    11041 tattggtgca atccctcaat acggtatcac aggttgggaa ttgcatccat tagttgtaga
    11101 aagctcccga cgaaagaacc aaataggtac tcgattagtc aattacttag aaaaagaagt
    11161 agcttccaga ggaggaatca cgatttattt aggtacggat gatttagacc atggaacaac
    11221 gttaagtcaa acggacctgt atgagcatac atttgataaa gtggcttcca tccagaacct
    11281 tcgtgaacat ccgtatgaat tctatgaaaa attaggttat aaaatcgtag gtgtcttacc
    11341 aaatgcaaat ggctgggaca aaccggatat ttggatggca aaaacgatta ttcctcgacc
    11401 aaattctcaa taataaaaag aggttgtgac agaagtaatc agcttcaaga aataagaagg
    11461 aatttacgaa aattacttct tgaatttttg tgaaattcca acttatttcc gaagaagcta
    11521 cttttggaac accgtgtac
//
LOCUS       DAZAFF010000087        11298 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000087 DAZAFF010000000
VERSION     DAZAFF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.088"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACG9BB_002263"
     tRNA            12..84
                     /locus_tag="ACG9BB_002263"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACG9BB_002264"
     tRNA            88..160
                     /locus_tag="ACG9BB_002264"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACG9BB_002265"
     tRNA            168..249
                     /locus_tag="ACG9BB_002265"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACG9BB_002266"
     tRNA            267..339
                     /locus_tag="ACG9BB_002266"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACG9BB_002267"
     tRNA            351..422
                     /locus_tag="ACG9BB_002267"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACG9BB_002268"
     tRNA            439..524
                     /locus_tag="ACG9BB_002268"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACG9BB_002269"
     tRNA            535..608
                     /locus_tag="ACG9BB_002269"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACG9BB_002270"
     tRNA            618..691
                     /locus_tag="ACG9BB_002270"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACG9BB_002271"
     tRNA            711..783
                     /locus_tag="ACG9BB_002271"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACG9BB_002272"
     tRNA            811..884
                     /locus_tag="ACG9BB_002272"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACG9BB_002273"
     tRNA            898..971
                     /locus_tag="ACG9BB_002273"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACG9BB_002274"
     tRNA            995..1084
                     /locus_tag="ACG9BB_002274"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACG9BB_002275"
     tRNA            1097..1170
                     /locus_tag="ACG9BB_002275"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACG9BB_002276"
     tRNA            1189..1264
                     /locus_tag="ACG9BB_002276"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACG9BB_002277"
     tRNA            1266..1336
                     /locus_tag="ACG9BB_002277"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACG9BB_002278"
     tRNA            1350..1423
                     /locus_tag="ACG9BB_002278"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACG9BB_002279"
     tRNA            1448..1535
                     /locus_tag="ACG9BB_002279"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACG9BB_002280"
     tRNA            1557..1628
                     /locus_tag="ACG9BB_002280"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACG9BB_002281"
     tRNA            1651..1740
                     /locus_tag="ACG9BB_002281"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACG9BB_002282"
     tRNA            1753..1826
                     /locus_tag="ACG9BB_002282"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACG9BB_002283"
     tRNA            1845..1917
                     /locus_tag="ACG9BB_002283"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACG9BB_002284"
     tRNA            1921..1993
                     /locus_tag="ACG9BB_002284"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACG9BB_002285"
     tRNA            1998..2068
                     /locus_tag="ACG9BB_002285"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACG9BB_002286"
     tRNA            2081..2154
                     /locus_tag="ACG9BB_002286"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACG9BB_002287"
     tRNA            2166..2237
                     /locus_tag="ACG9BB_002287"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACG9BB_002288"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACG9BB_002288"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFN2838188.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACG9BB_002289"
     CDS             3826..5298
                     /locus_tag="ACG9BB_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFN2838189.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACG9BB_002290"
     CDS             5381..6169
                     /locus_tag="ACG9BB_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN2838190.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACG9BB_002291"
     CDS             complement(6236..6904)
                     /locus_tag="ACG9BB_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFN2838191.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACG9BB_002292"
     CDS             7019..8731
                     /locus_tag="ACG9BB_002292"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2838192.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ACG9BB_002293"
     CDS             8734..10506
                     /locus_tag="ACG9BB_002293"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2838193.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>11298)
                     /locus_tag="ACG9BB_002294"
     CDS             complement(10588..>11298)
                     /locus_tag="ACG9BB_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2838194.1"
                     /translation="TGMIFKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYLSK
                     EEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYE
                     LKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAY
                     NQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTH
                     TTKNMQNQLVEKLGKIEI"
BASE COUNT         3250 a         2024 c         2555 g         3469 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa
    10861 aatgtattta tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat
    10921 ttctttagca ttacttgata attcaatggt tctataagaa cctgtatttt tagttgttgt
    10981 tttaattttt aactcgtaat cataagtacc agaaaccgta attttattac catcataatc
    11041 agattcagtc aaagatatca attcaccata acgtagtcca gttaaaaaca aaaatttagt
    11101 aattaaagaa taacgtgact gttgatgtga acttttttgt ttactagcaa tcatttgaac
    11161 ttcttctttt gataaataac catctttttc tttttttatt tcttcgtttg tcttaggttt
    11221 tcttgctaaa gaaaccccat ttacgggatt atattttaat ccatatcgac ggttggcgaa
    11281 tttaaaaatc attcctgt
//
LOCUS       DAZAFF010000088        11283 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000088 DAZAFF010000000
VERSION     DAZAFF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.089"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..1650)
                     /locus_tag="ACG9BB_002295"
                     /pseudo
     CDS             complement(15..1650)
                     /locus_tag="ACG9BB_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2089..2958)
                     /gene="scrK"
                     /locus_tag="ACG9BB_002296"
     CDS             complement(2089..2958)
                     /gene="scrK"
                     /locus_tag="ACG9BB_002296"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN2838195.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2978..4099)
                     /locus_tag="ACG9BB_002297"
     CDS             complement(2978..4099)
                     /locus_tag="ACG9BB_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFN2838196.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(4101..4496)
                     /locus_tag="ACG9BB_002298"
     CDS             complement(4101..4496)
                     /locus_tag="ACG9BB_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2838197.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(4515..5312)
                     /locus_tag="ACG9BB_002299"
     CDS             complement(4515..5312)
                     /locus_tag="ACG9BB_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN2838198.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(5314..6102)
                     /locus_tag="ACG9BB_002300"
     CDS             complement(5314..6102)
                     /locus_tag="ACG9BB_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN2838199.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(6116..6592)
                     /locus_tag="ACG9BB_002301"
     CDS             complement(6116..6592)
                     /locus_tag="ACG9BB_002301"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2838200.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(6664..9321)
                     /locus_tag="ACG9BB_002302"
     CDS             complement(6664..9321)
                     /locus_tag="ACG9BB_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFN2838201.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNIRKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(9385..9744)
                     /locus_tag="ACG9BB_002303"
                     /pseudo
     CDS             complement(9385..9744)
                     /locus_tag="ACG9BB_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(9922..10242)
                     /locus_tag="ACG9BB_002304"
                     /pseudo
     CDS             complement(9922..10242)
                     /locus_tag="ACG9BB_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(10358..10948)
                     /locus_tag="ACG9BB_002305"
     CDS             complement(10358..10948)
                     /locus_tag="ACG9BB_002305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838202.1"
                     /translation="MNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLF
                     PHEDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEI
                     QYEGIGFTLIDLKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMEN
                     NINYIDSGSDLLIRWQVITNFLMELKKYSNTQTKFS"
BASE COUNT         3602 a         2228 c         1630 g         3823 t
ORIGIN      
        1 tgaaaaaaat gtaatcagat cattattttt atatgttgta acttcctgat aagaaaacta
       61 attaaaagaa agaggtaact acataaacta attaaaaaag ctaacttcat agttggagga
      121 taaatggaag aaacttcaat gattgtttct ccttgtgttc cttcaatcga cactagtcct
      181 attatcaatc tctctttttt gtccatttac ttttgcgact gttccccaat aatcaaatac
      241 aggaagcgtt attttttgtc ctttagattc atgtactgta tagactattt tcgaatccat
      301 cacttgataa ggcatagtat gccattcctt gccaattctt acttcatgat tctgcaaatg
      361 ttctttgtat tgtaatgctt ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt
      421 cgtgtaacga ataagatttt cttgattcat taccaagtga tctctctgag tgacaacttt
      481 atgttcatag tttgcttttg ttgcagcaga tacagccaac agtaataaaa caattgccgt
      541 tatattaact acttttcttt gcttagctga tgtgctcttc atgaaaacga acgataaaat
      601 caacgagcca aacagtactt gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat
      661 gggtgtattt tgtaataaag accatggaaa aaatttggtg gataagagcc aagagactac
      721 cgcactaagg taaatcactt tttctttgcg attaaatcct ttaaaaaaga agagtggaca
      781 gatcaaagaa ataataccaa tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc
      841 accaatcaaa ctatccgaga catttaatgc ttggtgttgt aaggtcgttt tatctggctc
      901 attaatcgat tgaaccatga tttgtcggaa catcggatac caaaagtatg ctgttaaaag
      961 aatcgtcatt cctactgcct ttgacaactc cacaatcaac cggatgcttt ctttttttct
     1021 tgtttcactt actacaaagc cgcctaagac tagtaagcca acggctatta cactcataaa
     1081 agtggaaaga acatgtgtat aaatgagtaa gctcatactg actgctaatg gaattgcaca
     1141 tggctttttc tcataaatca gtgcgtaagc ataatacata actatcggta taaccgtaag
     1201 tgcaatcgct tcagcaatag cagaacgatg ataaatatca atcgtacggt acccataaaa
     1261 tgtatataaa cagctaaata taaaagccgc atcgattctt tttaaaaatt ttagtccata
     1321 atgaaacatc agcatgatag tacaaacatt caatagccaa acatataaga tataactaac
     1381 gattaaatta ttacttatcc aataaaaaat gactgccgga aaaaaggtca agtagggata
     1441 aaaataattc actccgctcc cataatgatt aaacgtcgta aaattgattg gaccagaaaa
     1501 aattgaagat aaccctttga ctctagcaaa atggaaggca atatcccccg atgtagaaat
     1561 agtatgaaca tttctaaaat aattgattga ataaagtcca gcaataattg ctaggagaat
     1621 ggacaatatt atttttttct ttacttgcat tacttaatta tatactcctt tcatggattg
     1681 ctttatctag tataacttaa attttaaata aatcgttatt tatttttact ctcacttaaa
     1741 aaagcactaa aattataaga gagggaggta atggtctgaa caccaagaat tgttccaaca
     1801 tcgaattgct taactaatga gttcaaagct taattatgtg gctcttatat ttaccgagcg
     1861 tggattgcga tctctttagc gaaacaaaca tctttcataa aaaaaacagc catatccgct
     1921 ttcttggata tgactattta ctactacttt actatctttc attcttcttg tctcacttat
     1981 gataaggcgc tgggtatcat gagtgaatga tattgctgaa tacaaatagt ccgaccacat
     2041 taagttgatc ggacttcata agcattttat tgactaatac ttttttgctt aagaattgat
     2101 tctaacatct tttgctaaag ttaagcatcc tatagttcct gcattatctg ctaacttagg
     2161 tgtgacaatg tattcttcga tatttggcgt atcgacatag ccgttaatta gttctaaaaa
     2221 acgtctttgt actttctttt tcagatgttc ttgttgcatg acaccaccac caaaaattat
     2281 aatatctggt gaaagcatca gggtcgtatt acaggcacat tgtgcaatat aatccgcctc
     2341 gatttcccag aacggatctt ctatagaaag atcttgtccc ttggttcccg tacgcatctc
     2401 gatagcgggt ccagatgcca acccttcgag acaatcatga tgaaaaggac aagtaccaga
     2461 gaaagtatct cctgaatgtc tcttaactaa cgtatgcccc atttctggat ggctaaaccc
     2521 ttcaacaaaa atcccatctt gtatagctcc tccaccaatt ccagttccaa cagtgtaata
     2581 aacaacactg gataaacctt ttccatgacc tgaaacatac tccccgtaac aagcagcatt
     2641 cacatcggtt gtccaggcaa ttggaatttc gaaatgctta ttcaacgttc caacaaaatc
     2701 aaaatttttc caggcagttt ttggagtaga ggtgatatgt ccataggtct ttgattcacg
     2761 atggatatca atcggtccaa atgaccctac tccaatgctt tctaattgtt ctttatagtg
     2821 attaaagaaa tcaattacca aactcattgt ttcattcggt gtagttgtag gaaaactcac
     2881 tctttctaaa acctttaaat catcattgcc aatggcacaa acaaatttag tcccacctgc
     2941 ttctattgag ccatacattt cttactccac cgtcctttta tctaggaatt cgttcttccc
     3001 tgtctaatag tttttcgaaa aactcatgtg gttccttttg gtaccaatag gcaacagaag
     3061 aaatatcgtc ttgacgctca taaagcttga tgtcatcatt tcctagggtt tggaaagtaa
     3121 cacgtaaatt ttctttgaaa ctaatggggt cgggtaaatg ccagcggtac aacccatgca
     3181 tcggtaaagc atcatcgcca aaaccatgaa ctggatttgg attaggtttt ccagcactaa
     3241 aataatcccg ggtgtgatct cttttagatt gaaaaggata gcccataaat agcgtattaa
     3301 aattttgagc atatgccctt tctccaaagt tctttttatg gaaagcccaa gcgccgccaa
     3361 aataatcttc tgctcctgta gatgttattg tagggaaatc ttcatctcca tctatataaa
     3421 acttaaattc tccttccccc caccagtatc tttccaatgc gcacaaagca aagtaggttc
     3481 ctacataata cccagtgcct ttgattccat caataagggt gtaatctttt ccttttgtcg
     3541 tgatgggttc acggttccat ttagcatgaa aatacatcgc atcatccggt acttcagtca
     3601 atgcataatt aaatgtataa aaaatgtgct taataaactc aggatgttca ttcgttaaag
     3661 taatcttagc tttttttcga aatggcatct caaaatagca attcatccct gatgttggat
     3721 tcactacgat tggtaaggaa ttcacttcgc atcttgtccc aaacccatta caaaaaaaat
     3781 ctcctaatgg actttctaca gaaggagtgg attcatcatc ccaataaata cgcaaaatta
     3841 aatcacgtaa gacgaaactc ccagcgattg ttttgtctgt cactgtcatc caaaaatgac
     3901 gtaaaattcc agggccttcc atttctgcta aagtaattgt ctctccagca tttatcggaa
     3961 cagaaggtga cccttttctc ccaattccta gatgactcgc tgtcattgcc gctttccctt
     4021 tttcaccatt tttattttct ggtgaaatag ttctactttc ctctcccttg tggagccata
     4081 tattatttaa acaattcatt ttattcgtcc tctatccttt cttcactttt ttgattattc
     4141 atatagatga tttgttgttg tgcctccgca ataattgctc caatatctaa attatcattt
     4201 cctaactgca aagcgattga cattgccaag ggaagattca ttcctgcaat tacgtgcact
     4261 ctgtcagaaa tataggtaaa aaacttttga tttacacttc ctccagccat gtcagttaaa
     4321 acaattattt cgtctgtagt atccactaac tctttaattt gttcatctat atcatcaatt
     4381 tcttcgacat atgcacataa gctactaatt tgattatgca tacctgttaa aaactctagt
     4441 gtctctttaa aaccctcagc tagtattgaa tggctagcaa tgatcaatct tctcataatt
     4501 caaatccttt tcctttacgc taatattcca aatgcagcca taaccatcgc aaaaacaata
     4561 acgagtagaa tcaaaatggt catactgatt tttttcttac tgagtagata atatgctgca
     4621 gcagtaataa ttactggaat taatgttggt aaaattttgt ccaacattgg ttggattttc
     4681 atactaactt caccaaaagt gaatttaagt ggcgttgaaa cactgataac tgaagcaatt
     4741 aatgatccaa ctactgttag ccccaatact gaagcagctt cagttaaata attaatcttt
     4801 tcaccgtatt cagtaacaat cttcattccc tgcttataac caaattcaaa gcctctcatt
     4861 cggaaaaaga agattaccat gattaaaatg aaccataaaa agattcctac aggattattt
     4921 tctaaagcca tataagccgc aattgatccc atgatggttg ggatcatcgt catcaaaaga
     4981 gagtccccaa taccagcaat tggacccatc gtaccaactt taaagtcatt aatcgcatct
     5041 tttgcttcta atccttgctt ttcctccatt gcaacacaag cacctaaaat aatggctgcc
     5101 agccaaggct gtgtattgta atactgaaag tgattattta aagcttcgat gtattcatca
     5161 tcatctttgt ataattttct taaaataggt gacaatgcgt aagtcacaga tgcaccttgc
     5221 tgtgtttgat aattaaaggt acaaactgac atcaaccaac gatagttcgc tttcctaaga
     5281 tcctttttcg ttacttgata tttttcgctc attttattca tcctccccaa taaacccaac
     5341 atcagaagct cccgcagaag cagtgatttg tgcatctttt tcagctctat gatacacatt
     5401 aaatgctaaa gcgaatccta tgatcgccgc tccaataata ggtaccttca aaaaagcggc
     5461 taagaaaaat ccaacaagta aataactaat gtatcgacct gtaggcataa agcgtaacaa
     5521 catcgcaata ccaacagcag gcaacatttt tccggctaat gttagcccat ccataaacca
     5581 attaggtaaa acatccaaaa cgtaattaac agcagactgt cctaaagtaa cggcaataaa
     5641 tactggaatt gctgcagata aaccaaagaa aattggcccc aaccacaaaa cactattcat
     5701 tttcttaaat tctcgtttat tcgcatattc ctgtgatttg cgaacaacaa aaccatttaa
     5761 gattttgaca attacatcta gttgcacccc taacatacct acaggtagtc ctattgctaa
     5821 tccggcttct actccaagct ggctaccaaa tgccgttgca ataattgcgc acgttccata
     5881 gtccggtaca gatgagccac caaaaccggc aactcctaat gacatcaatt gaactgtacc
     5941 gccaactatc aaacctgttt ttatatcacc catcaaaatt cctgtgaata atccccaaaa
     6001 aactgcgtaa tttacaaaga tcatcggaat cccataataa tcaacatgtt taataaatgc
     6061 taatatagtc agcataataa tttgaaaaat agtaaaatgc attatcctta ctcctttatc
     6121 ctaattttga ttgaatttcc tgtgccttat ccgccggaac aaattgtgta acaatttcta
     6181 caccagaatc caacaatttt ttatacgtgc tgatattttc atctgatgca taggctctag
     6241 aagatagctg taatgcatta tctttaacaa cagtaccgcc aacaattagt cggtcaattc
     6301 taataccgtt atctactaaa tgaaggattg tttccggctc tttcacgatg acaaatactt
     6361 tttcctgatc gtatttcccc tttttaaaat tcgcaatcgc tgtttcttca gtgataattg
     6421 atacagccat tccagcaggt ctagctaaac gcatacttga tttttggatg tcgttatttg
     6481 cgacagaatc atcgatcacc atcactctgt ttgctttaga cattggtgcc cattgcgtga
     6541 caataatccc atgcaataaa cggttatcaa ctctcgacat aacaatactc ataaatactt
     6601 cctcctttat ctttcatttt cttacaagca attaccatgc caaaacaaaa agaacttgat
     6661 aaatcaagct ctttttacat aatcataaac gtatttaacc tcatgaatcg ggatgtcgac
     6721 actataataa tgttcaacga cactgaaaga ttgcttaaaa tgtgtctgaa atttttgaaa
     6781 ttcctcactc gtttcattga aataaatttc atcttccaaa gtattttgtt ttactaatcg
     6841 ttcaattaag caactaatgt gaacatataa tccgaaacac gtattattat caaatgaaat
     6901 tcctagatca ttttgtaaga tgtatatagc atctgaaacc cgttctaata atttagtcgg
     6961 atttaatatg gtcaattgct ccattaaatt cgttagacta aaattcttca gaatattatt
     7021 gttcaaaact ttcagttcat tatcattaaa gtgtctattc aattgattgg agaattttct
     7081 tatattcatt cctaaaatca tatcttcaat tgaaaagaat tctacatctt ctagatgtgg
     7141 atttagtgtt ccaacaataa aaagaatatt gttctcttta cgaagctccg ataaatagtt
     7201 ttcattcact aaagagtaat agtcacacgt ttctatgaca agatcagtat cccttggaaa
     7261 gctatcttca agtatccttt ttaatttaaa cgaagttcct aatccgcttg cacaagaaca
     7321 gacaatcata tccttcttaa tatttgatga ttcataaata taatccacat tttgattttc
     7381 ttttacttgt tcaagaatct gtttaagcgg taaatctaat aatatctgat ttgcaacatc
     7441 aatagccatt cttgttgaaa cattgtttag tagtccgaat tttttttgag cgtctagttc
     7501 caaatttaga taaattttct ccagtgaacc catatcaaca agtaaaataa tttcttcata
     7561 tccggtacac tcttgtaagt atctattaat tttgtctgca atctcactac ttgaagtcat
     7621 tagtggcata tcaatggcgt cgtatatata tctctcaacg atcctattag cagtacttgc
     7681 tatacaggtt gccgttagat ctccatgagc caaaatcagt gctaaacgtt tttgctgaaa
     7741 ttctttacta cacattaact taaataaaat aaccaaaaca tttttttgaa tttttgttaa
     7801 ataaatatcc cctatttgaa taaaacgatt gactaattga acttctcgac tacaccaact
     7861 attaatatca ttgatgaagg aatataattt atcgtccgat gttaattgga tttgataaat
     7921 ccaagaattc aaaaagatag acgttatttg aagataataa tagtcaaatt ctatttgaaa
     7981 atctctctct atttccttaa catactgtct aaatcgaatc tcttgatagg tgggatatgt
     8041 taaagctgaa ttattttgga tatcaacatt cttttgtcta aaatatctat catatttctc
     8101 aaaaagctgc tgcttatcag tgtctttctt ccacgtttga taaagatact ctataagttc
     8161 gttgatatta aaaacttcgt ctactttttc caaatcatcc atttgaatat attttaattc
     8221 attctgtctt ttcataacct tcatgccgtt gaaattcacc cattgtggta aatgatacat
     8281 ctgaatataa agttcttcca aatcatctaa gaaataaaag gcattggcac aggtcaattg
     8341 aatgatattt ttcaaattgc caacattttc actaaagcga tagtttaata aaaaatctaa
     8401 taattgttga gatatcagta ttttctttgc tagtctttct tcttctgatt taaaaaatat
     8461 cttgattaac tgttttcttt cataaagaga acgactgtct aatgttggaa gtgttagctt
     8521 tacaggtatt cttctcaaca aagttgataa tagtttatcc tcaggtggtt ctgatgttgc
     8581 aaatataatc cagcaacttg atgtgtacca gttttcatta tcacccatcc gatgaaattt
     8641 cctcgtatcc ataaactgaa aaagtttctc ctgacattca ttgctcaatg catgaatttc
     8701 atctaaaaat aagactccat tattagctaa ttctgctaaa ccctttcttc catcttcggc
     8761 tcctgtatat gcacctttta catgcccaaa aaaattggac aagaaaagtt ctggattatt
     8821 ggcatattca ctacaattaa ctgaaatgaa agatgccttc tcttctatat aattattggc
     8881 caacaaatcc ttatacacat aatttgctaa atagcttttt ccagttccag tctcgccatg
     8941 aagtaaaatg ggtaagccat ttggcggata gtagattgct gctttaattt gagcaattac
     9001 atttcgcaga cttccttgac ttccgataac cctttctatt ccaaccctaa aagagctatc
     9061 ataccatttt tccgcttcct ctgaagagct aaaaatttgt tgatttggaa ctttctccgg
     9121 taaagaaaag aagattactg gacgagtatt gatttttcca aattctccat cttttacgaa
     9181 ttcattcaaa tattgagaga caatattacg actaacattt aatttttcag atattttctg
     9241 tgctgtattg ttcccccatt tatttttaat ctcagagttc tctatgacat aattgacaat
     9301 gcgttctttt aattcttcca tcacagcacc tcctcgacac tattcgaatt ttgccgacag
     9361 tatttttcca taaatatgac ttgctaaaaa ggctttgttt agatcattca attggaacgt
     9421 tgcttgtttt gcttgatccg ctaagacgtt gaaaagatac tctggcgaaa tcgcataccc
     9481 caaaatatca acgattgttg caatcagatc ttcttttgat tcttcatctg aaagtaattc
     9541 acgatacaac atcgtttgtt tgcttactgt gtcatattcc tctaaggatt catctgctaa
     9601 aagaaccact tgttccaaca aacggtcgga taaatattta tagaaaatca gccccaataa
     9661 ataattttta tattctgacg catccatttt actacggaga ttatctgccg cactaaataa
     9721 cttctgattt aattctgcac ccaaaaagat acactcctat ctcttattta aagttgctat
     9781 ataccctctt attttagcat actcagtttt agaaaaagca aaaaaataaa gaaacttatt
     9841 cttgataaca agctacgtga cgtatatccc taacacaaaa atagccatat cctttggttt
     9901 ggatacggct atttgatatt actcattcaa ttatcaaatc acagtaggtg tgaatctcat
     9961 cgggatcggc aatatctact tctgattagg atcaaaggtg attttctcaa taaaggtgtg
    10021 atacaaagct ttaacttgct tcttctcact ctggataaga aagcaatcca aactagtcaa
    10081 tatctgttgg acatctttga cttgaatcgg ttccccttgt tgttgaaaga tccctaagat
    10141 ttcattctct cgaatttgta actgtcttcg tttttcagtc aattcattta aacgatcttt
    10201 taacatcgga aacaattcgg gactatcttc caaagcagct tcctgttttt gctctaatag
    10261 ttagattttg cgtaccattt tcttctttta ttttccttcg attgctgatt ttaatatagt
    10321 ctaaaggaaa tttatattta ttgtattctt tataacttca agagaacttc gtttgagtat
    10381 tactgtattt ttttaattcc ataagaaaat tagttattac ctgccaccta atcagcaaat
    10441 ctgaacctga gtcaatataa ttaatattat tttccatctc caacattgta agtactgttg
    10501 gtgttaatat agggactttg tacaattgta tttttttgat tttatcatct gcacaaagga
    10561 gattttttaa atctaatcct tcttttattt caaaagttga attttttaaa tctataagag
    10621 taaaaccaat tccttcatac tgaatttctg aaaatcgaat ttttctccca gttgatagat
    10681 caaggggcag aaaacctttt attacattgt gagtgtcacc atcatttaca atgtcattag
    10741 gaattttgat tttagaattc tgtggagcac ctattggaag ataataattc attataattt
    10801 tgtagtcttc atgaggaaat aaagtacttt taggattgct aagatattct cgtaccgaat
    10861 ctaagaatgg cttatccatt gaatcttcat ccccatttaa actaaaaaac attcctacaa
    10921 ttgcttttgc aactctaagt gaattcattt tctttattga atgtgttaaa tcagacttac
    10981 cattctttaa acttgtattg ttataaagga aatttttatt gtcttctgca aatctaatat
    11041 attcctcaac atactcacta ccaaaaaaac tattacattt actacataac gtattggaat
    11101 ggcaacagtt tttttgacaa attttataag gtgcagaact tctttccgta tgctattccg
    11161 attgtccttg acaatgagcc tcctcggatg tgacgatctt cggcaggagc taacagttaa
    11221 agttagactg cctcgctagc gttagcacat ccgagctcat tatcaaggat tcgctgcgcc
    11281 aat
//
LOCUS       DAZAFF010000089        11245 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000089 DAZAFF010000000
VERSION     DAZAFF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.090"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..534
                     /locus_tag="ACG9BB_002306"
     CDS             <1..534
                     /locus_tag="ACG9BB_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838203.1"
                     /translation="ISFFKEYLEIINFRLKTKKESVIILVYFQGGNLMIKKLFLLSIL
                     TLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCS
                     NRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAF
                     SFAREDEDNPKTYDILA"
     gene            complement(595..960)
                     /locus_tag="ACG9BB_002307"
     CDS             complement(595..960)
                     /locus_tag="ACG9BB_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFN2838204.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1036..1281)
                     /locus_tag="ACG9BB_002308"
     CDS             complement(1036..1281)
                     /locus_tag="ACG9BB_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2838205.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1351..1605)
                     /locus_tag="ACG9BB_002309"
     CDS             complement(1351..1605)
                     /locus_tag="ACG9BB_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838206.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1769..2092)
                     /locus_tag="ACG9BB_002310"
     CDS             complement(1769..2092)
                     /locus_tag="ACG9BB_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838207.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2279..2352
                     /locus_tag="ACG9BB_002311"
     tRNA            2279..2352
                     /locus_tag="ACG9BB_002311"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2313..2315,aa:Ile,seq:cat)
     gene            complement(2737..3234)
                     /locus_tag="ACG9BB_002312"
     CDS             complement(2737..3234)
                     /locus_tag="ACG9BB_002312"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFN2838208.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3274..4083)
                     /locus_tag="ACG9BB_002313"
     CDS             complement(3274..4083)
                     /locus_tag="ACG9BB_002313"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN2838209.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4417..5274
                     /locus_tag="ACG9BB_002314"
     CDS             4417..5274
                     /locus_tag="ACG9BB_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFN2838210.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5267..6169
                     /locus_tag="ACG9BB_002315"
     CDS             5267..6169
                     /locus_tag="ACG9BB_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFN2838211.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6202..6552
                     /locus_tag="ACG9BB_002316"
     CDS             6202..6552
                     /locus_tag="ACG9BB_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN2838212.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            6601..7524
                     /locus_tag="ACG9BB_002317"
     CDS             6601..7524
                     /locus_tag="ACG9BB_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFN2838213.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            7602..8495
                     /locus_tag="ACG9BB_002318"
     CDS             7602..8495
                     /locus_tag="ACG9BB_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN2838214.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            8533..9096
                     /gene="amaP"
                     /locus_tag="ACG9BB_002319"
     CDS             8533..9096
                     /gene="amaP"
                     /locus_tag="ACG9BB_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN2838215.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9110..9298
                     /locus_tag="ACG9BB_002320"
     CDS             9110..9298
                     /locus_tag="ACG9BB_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN2838216.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9311..10213
                     /locus_tag="ACG9BB_002321"
     CDS             9311..10213
                     /locus_tag="ACG9BB_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN2838217.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10245..10487
                     /locus_tag="ACG9BB_002322"
     CDS             10245..10487
                     /locus_tag="ACG9BB_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN2838218.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(10743..11114)
                     /locus_tag="ACG9BB_002323"
     CDS             complement(10743..11114)
                     /locus_tag="ACG9BB_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838219.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3981 a         1925 c         2132 g         3207 t
ORIGIN      
        1 ataagctttt ttaaagaata tttagaaatt attaattttc gtctgaaaac aaaaaaagaa
       61 agcgttatta ttttagtcta ttttcagggg gggaacctta tgataaaaaa actattttta
      121 ctatcaatat taacgctagc aatggcaggt tgttctgact ctggcttagc aactgaactg
      181 atcacaaaat caactgaagc cacgacagag gagttaaaat gtggaaacga tcacagtgac
      241 tacagaggct taaatcgacg atgcatggga atattaggca gaagcaattg taagcatcga
      301 tgtagtaatc gaagaaggtg tagaagaagc aacagaaaaa tcaaaagaaa gatcaaaaga
      361 agcaatctgt gctgtgcatc gtatacagac gtcgaagatg cggtactttt aaaccacgtc
      421 aggagatgtt catttatgtc tagggaagtt ggcgatgaac caaaactaga gtacgctttt
      481 agttttgcac gtgaggatga agataaccct aaaacttacg acattttagc ttgatctaag
      541 atggttaaag ctttttaatg aagtaagagg agatagaagg cttttctagt tatattaggt
      601 aagataaatt cctttttgct ccaaatttgc taaacattct gctagataaa cttcatcgtg
      661 ttccggataa atcggcacgt tgcgctctac agtaggtaaa tcaaaaaatg cttcgcatga
      721 tttgccacga tacataggat ttttccactc tggtgctggg ctgtaaccac gtttttccat
      781 ttcatccatg acaagctgat gatattggaa taatctataa ggagaatagt gaaaaacata
      841 atccacagta gcatgtttct ttccccagcc atttcctctt aatgcacagc attcccgatg
      901 ttgtcctaaa agctgttgac gaggaagctt tgaaatcaat ttttcatgcc ataatcgcat
      961 agaaatgtct cctttatata tagattgtta accttagacg ttaaatgaaa aaaccagcac
     1021 caaggctgga aaaaatcact tcagtttgct tgctgtttct gagcgaccaa ttaggtaatc
     1081 aacggaaata tcgtaaaagt cagcgatata gattaattca atgatgcttg gaatttcttc
     1141 tccttcttcg tacttacgat atttgaataa aggaatactt aacgcgctag aaacagctac
     1201 ttgagataaa gatcggccag ttcttaagaa attcagacga caacgtaaag tatccagtaa
     1261 aatagcttct tcgatactca tttaatcacc tttcattata gtagaatagt tataatgact
     1321 atagaaagct acacatgcaa tcaatagtac tcaatgtttt ttatttaaac gtatcaaata
     1381 aagaatgacg atcaataaag acaaaaccag tcctgataat tcttctataa aaacactatc
     1441 gggttcaaag tagttgtaat taaaaacttt ccacataaag tagatagccg gaagagtcaa
     1501 aatagctaaa taaaaccgtt ctttgttcca attcataaga taaacaatac ttgcgaccag
     1561 tgcgaccgca ggcacccaaa acaaaactct caccaacgta atcatgtaac ggactcctca
     1621 acgattcata taataaaaga taacatgtta ttgcatacat cacaaatgaa atgctaggaa
     1681 aataaaagaa gaatagatca atgaaaaatg aaaaagcaga acagttttct gctgataagc
     1741 aggaactgtt ccgctgaatg tgtcttatct atcgagaaaa aatcaatgga ttgatatcta
     1801 agtaattcaa tacatataaa aagaggacga tcgcattcac taaaaaggaa agaatgatga
     1861 tcccatagcg aatggaaaca tatttaacaa atacttttgt ttgaatacgg gcatatcttt
     1921 ttcctttata gctgagcatc atgacaagaa gcaaaagggt aatcacacct tgaaaaatca
     1981 ttccaataaa cagaattcct gaggtttgat aatttaattg gagaaaataa gctgctgccg
     2041 caactaaatc gaataaaatc gcaataaatg gcaaaataat cactcctaac attgagaata
     2101 aataaaagaa taacattttt tctaaaataa gcaaataaaa attaaagtaa caaaatagtt
     2161 atgacacaag taaaagaaat aagaataaca atattatttt acttatattt tgaaacttgt
     2221 gagagtttgt cattcgtgct atagttgaaa agtaacaaaa ataaatttta tttatgatgt
     2281 acctttagct cagttggtta gagcagacgg ctcataaccg tccggtcgta ggttcgagtc
     2341 ctacaaggta cataagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca
     2401 agtaaaaaca gcttcttatg tttttacttg gctgttctta ttactgcaga tgcaagcttt
     2461 tgaataccgt ttaaggaaga ggttgtgaaa tcagcgtttt gacctgagta ttagcttaac
     2521 aaacggaaaa atagttcctc atattttgcc gtttgttaag ctaataccga gggtcagctg
     2581 attgaacacc atttaagaaa gaggtcgtga gcctgccgtt tcactctgtg tcataaaaaa
     2641 gccaatctac tttgatagaa aaagcacgta ggactaaaac agttctgcgt gcttttttta
     2701 ttgtgtgtta aatatcgtaa atgaagatag atatttttat gattcgacga tagagacgtt
     2761 gaaatctttc aacagctcta aagcggattc tccgtgtttt ctactggaga ctaacattgg
     2821 atcaacaaaa aagtctgctt caggaaaagc actctgcagg cctatcagat tagagagtac
     2881 acacaagttt gtttcaatcc cgactacttc gatctctttt tgttcttctt tgctttcaaa
     2941 taaagtttcc tggatttcag ctaaagtttc aggtggaatc gtaaagtaat actttttaat
     3001 cactcgttca ttttcttgtg gagaaagaag agggatcaat tgcaaatctt ctcgttcttc
     3061 ttctttgtcc ttcagttcaa ctggtatatc ccgtgtgaag agaatatatt cattattttg
     3121 gcgtgcttta gctaatcttt gagcaatacg ttctgccaat tgatctgact ctggaatata
     3181 aaattcgctg tttggatcaa gaatatgatt ttgcatatca ataataacta acattttctt
     3241 tcctcctaca ttcatatcgt cattttattc tttttaatag aagtttttta aaatataagc
     3301 aacaccagct tcttcattcg tcagtgttac ttggtttgca atttctttta cttgtcgctg
     3361 tgcattcccc atcgcaatac tcaacccgct gtaattcaac atagcaatgt cgttttcttg
     3421 atcgccaaaa gccatgatgt cactcgattc gattccaagg taatccgcca gaaaaacaag
     3481 tgaatgagct ttagtgatac ctgtaggcgt gatttctaag aaatgcggat cagagaatac
     3541 tgattggata ccatctgctt gcagatctct ttgtagcttc tgtagttttt cttcactctt
     3601 atctgttaat gctaatttaa gatagctaag agaaggatct tccaacatat ttaaaatttg
     3661 atcaaaagaa tggttctgta aattgaaatg ttcataataa gggtcatctt tgactagatt
     3721 tgattcatcg aatgtcagct gatcatcttg ggtttctcgg ctagcaatga tggatatttt
     3781 cgcttcttga gcatagaaga aggcttttct cagtgtatcg ctatctattt ttgaacgtct
     3841 gagaactttc gccatttttc catccttcat ttcagcaatc agtgaaccat tggaagaaat
     3901 caaatagcca ccgattccta attcggaaaa aatatcttta gcagaaatgc tgttccttcc
     3961 ggtagcgacg gttacgattc cgccattttc atcaaatttc cgaagtgctt ggatattttc
     4021 ttctggtatc tttccatttc gatcaagcaa ggtaccgtct aaatcaatcg ctgctagttt
     4081 cattttatct tcctactttc tttttacata tctattttcc tgttacctta agattaaatg
     4141 agaatttaaa aaaatgaaac taacttgctt tgtcattatg aattttttca aaaaacattc
     4201 ttaagaaaaa ggtggctata tatagtagtt tttggtcaat acctttgaat aaaaagccat
     4261 gaaaaacgaa tagttagcgc ttaccgatgc gttttgtgat aaaaaaattt taaaatattc
     4321 aatgaaaaca aagcatatat tttgctttaa aaccaaatga ggaatattct agatgtagaa
     4381 caggttagaa aaagattaga aaaaggagtg attttgatgg agaagagata cggcgtagtt
     4441 cctgtggtaa ctgcttcaga cgaaaattat gctccttact tgagcgtgat gatcgcaact
     4501 gcattagaaa attgcaataa aacaagacgc ataaaatttt atgtgattga cgatggtcta
     4561 tcagaatata gtaaagaagg attggaagaa acggtaaata aatattcaag taatgcaagt
     4621 atccaattcc tgacagtgga aaaagatatc tacgaagact tcctagtcag tgaccatatc
     4681 acaactaccg cttacttaag aatttcttta ccaaacttat tagctaaaga ggattataag
     4741 aaagtccttt acttagattc agatgtgtta gtactagacg atatcgtaaa actatatgat
     4801 gagccgttaa atggaaaaac aatcggcgct attatcgatc caggccaagt gaaagctttg
     4861 gaaagattag gaatcgattc cgatgacttg tacttcaatt caggagtgat ggtgattgac
     4921 attgatcaat ggaataaaaa agaaattaca gaaaaaacta tccattacct cagtgaaaat
     4981 ggcgatcgaa tcatctatca cgaccaagat gctttgaatg ctgtacttta tgaagattgg
     5041 gaacaacttc atccaaaatg gaacatgcag acttcgctga tttttgaacg tcatccagca
     5101 ccaaatgaaa aatatgaacg tcagtataaa gaaggaaatg aaaaaccttc gatcgttcac
     5161 tttacaggac atgataaacc ttggaatacg ttgaaagatc atccgtatac caatttatac
     5221 ttaaaaaaat tagcacacag tactctaacg aaagtaggcg agatcaatga ataaaaaaga
     5281 aatagcagta gtcgcaagtt gtaacacaaa attcgtacca catttagctg cattatttgt
     5341 ctcagtatta gataattgca acccgtctaa atttgttcgg ttctatgtga ttgacgatga
     5401 tatcgatttt gaaagtaaac agcttttacg cttctcagtg aaaaacgcac gaatgaactc
     5461 agatgtggaa ttcttgaaaa tcaataaaga attctttaca aatgtggtaa tcagcgatcg
     5521 tattccagaa acagcttatt atcgtattgc gattccggaa ttgttccgcg gcacagaagt
     5581 tgaacgtatc ttatatatgg attgcgacat gatcgcttta caagatatct ctaaattatg
     5641 gcgtcttgac ttcggtgatt caatcgtagc agctgttgaa gacgctgggt tccatcaacg
     5701 actggaaaaa atgaaaattt cagctaaatc aatgcgttat ttcaactcag gtttgatgct
     5761 gatcaatgtg aaaaaatggt tagatgaaaa tattacgcaa aaagtattag atttcatcga
     5821 acataatcca gaaaaattac gattccatga ccaagatgca ttaaatgcta tcttacatga
     5881 ccgttggtta ccacttcatc cgcgttggaa tgcacaaggc tatatcatgg caaaagcaaa
     5941 aaaacatcca acagcagcag gtgaacgtga atacgaagaa actcgcaaca atccttacat
     6001 tatccatttt tcaggacatg tgaaaccgtg gagcaaggat tttgaagggc caacgaagaa
     6061 atattacgaa aaatatgcag ggatgacggc tttccgttgt gtagctaaat tccctaaata
     6121 tccaaaatat gcaaaagttc aaccaaaaac agatgtagtt gttaaataat agaagaccac
     6181 aactgaagag ggggagtagg aatggaaatt acaatgtcta aaaatgcagt agagacgtta
     6241 attgaaaagg ttggggaaaa tacaaaaatc gcactcgcgc ttatcaatga ttccgatcca
     6301 tttctaaggg acaaaggtgc ttttgcaaaa ggcagctttt ttcaaatcat tccttttgtt
     6361 tcggaatttg gagaatatgc gactaaaatc gagcatccat tattggacat ctatacatct
     6421 aaattagagc aaaattattt cggtaaacga ttgaacatgg acttcaacaa acagcttgac
     6481 agcttttcat tagaaaatga aatcgcagta ctcgattata atatcaaatt aaagaactgc
     6541 ttcttcagct aatagtttcg agaaagtcca aaaaggaggt atacattgag atactacttt
     6601 gtggatgaag aaaagaatgt aatagaaaca gatgaaaaaa aatataattg gctcgtactt
     6661 gatagtgcaa agcccgaaga gattgagcaa gcaatcgaag aattcgagct tcctgatgat
     6721 attttcgtag gaaccgaata tccggaagaa gtttctcgga tggagcgcct ttatgagacg
     6781 aaactagagc atccattttc tttggtagtg attaatctag atcagtcaga taatcgtatc
     6841 gaaaaaaaat taacgccaat atcttttgta ctttccgata atctcttgat tacttgtatc
     6901 gatagaaaaa ccgaatttgt ggatcggctg ctgacaaagc atggaaatca tttaaactcg
     6961 tttgaaaaga tcatcacgta tacattatta gatatttaca cccattacgt gaaagaacta
     7021 agagagatga aaaagcgaat tgacgctttg gataaagcag ctcgtaaaac aacggaaaat
     7081 gaagaactgt ataaacaggc agatcttgaa agagacatcg tatatatcga tcatacctta
     7141 agagatcaac gggaaacaat ggatcaatta tggaattctg aaactttcat caaacgttta
     7201 gacgatgaac agctactcta tgatgtccat ttgagacagc gacaaacaga aaaaatgatc
     7261 gagatctatc gagatttatt agagagtatt ggtgatctat tcaacgggat gatggacaat
     7321 aatctaaatc atatcatgaa atatctagat tcagctgcat taatcatttc cgttccggcg
     7381 ttgttcgcag ggatttgggg aatgaataca ggaggattac cgggtaaaag ttctgctcta
     7441 ggctttatac tcgtagtctt tggctctttg atagcagcag tagttttagg ttaccattta
     7501 taccggaaag attatacaaa ttaaagaatc tatttaaaac aggtatcaaa caaagaaatt
     7561 tgttttggca atacaattta aaaactagga ggaatacagc aatgacagaa ccaaaattaa
     7621 tcgacccgcg aaacttgtac catacggaga aatttcctaa acaagatcag gatacaccag
     7681 ctttacagca gaatatgatt cctgttcctg attgtggaga agaatcctat gaaggcaata
     7741 atcaactaga aaaccgccgt gtactaatca ctggtggaga ttcagggatc ggtcgtgcag
     7801 cagcgattgc atttgctaga gaaggggcag atatcgctct tcaattcttc ccaggagaag
     7861 aaaaagatgc agaggaagta gcaaaatacg tgaaagatgc aggacgtaag attgtcttac
     7921 taccttttga tcttcgtgac gaacaagcac ctaaagaaat catagataaa gccgtgagtg
     7981 aacttggcgg gctagatacg atggtactaa acgcaggaca gcaaatttct tgtcagtcta
     8041 ttgctgagtt accacttgag caagtcaaag acacatttat gataaacatc atcgctatgt
     8101 ttgctttagt gaaagaagct gttccgcata tcccagcagg aggagcaatc gtaacaacaa
     8161 cttctgttca agcattcaat ccaagcgaac atttattaga ttacgcagca acaaaagctt
     8221 caatcgctaa ctttacgatc ggtttagcaa aacagcttgc acctaaagga atccgtgtaa
     8281 atggtgtagc acctggtcca atttggacac cattacaact agacgaagga caaccttctg
     8341 attcgatccc tgaatttggt cagcaaagtt tactagaacg tgcaggtcag ccaggagaat
     8401 tagcaccagt ttatgttttc ttagcatcga ataaagcaag ttatgttaca gcccaaattt
     8461 acggagtaac tggtggagaa gcgatcaact tataagaagt tgatcgtttc acccctatga
     8521 ggaggtgcga aaatgaataa aggtgtaaaa acgatcggag tgatcggaac attacttttg
     8581 ctatctgtgc ttttgtttgc gatactatta agtgcaacct atccattgcc ttttcagcta
     8641 gagaagtttc gattcttttc catcactaat tcatacgtgc aacaatatgt attttggctt
     8701 gccgttgcat ttgctatttt gacaattatc acgttacttg ttttcctgtt ttatccaaaa
     8761 acaatcggaa actttctact gaaacatggt gatggggagt tgacgttgga taaaaaagca
     8821 attgaaggaa tggttcgttc tcacttgcat gaagatgaat tcatccattc accaaaagta
     8881 aatgtcaaag caacgaaaaa caagatcgcc gtcaatgtaa aaggggagtt gaaacgaact
     8941 tcagcactca ttggtaaaac aggaacattg atgaaagaaa tcgaaaatga agtacaacac
     9001 atattgggaa caaaagagcc agtgaaagta tcggtgaaat acacaggctt tgaagaacca
     9061 gctaaacgaa gcaatgaccg caaacgagta gaataggggg ggaccaaaaa tgaaagaact
     9121 attttcgaca tacaagctcc ctatcatctt tggggtactt ggattgatat tagccgtttt
     9181 atggatcacc gtgggctttt tcaagacctt attgatcttg atcatggcag cgatcggcgt
     9241 cgctatcggt ttttatctga aacaaacgaa aatcttagat cactatttta aatcttagga
     9301 ggaaacatcc atgaataaca ataacaaaaa taataacaat aaaatgaaca accaaaaacc
     9361 agtgtctgca catccagacc caacgacacc aaaccaatca aatggtgtga acgatgaaaa
     9421 gaacaaaaca gcaccagtat ctgcacatcc agatccagta gtccctaacg ttcaacaagg
     9481 agtagaagag aagaaacatg atgcagaaaa gcatgcacct catcatccag gaactccaga
     9541 atctaaagtt ccagaacaac atgatgcaat cgatccttct aaacctgtgg aagagcaaaa
     9601 acatccttct gaccatgcga aagcacaaga aaaacttagt tctgaccaca caaacggaca
     9661 aggaaaactt ggttctgatc atacaaataa ccaaggaaag catgattctg aacaggaaga
     9721 ttcaatgcgc ggtgagctga attatgaaga taaagttgtg caaaaaatca tcggaatcgc
     9781 aattgaacaa gtagacggtt tattatctgc aaacggtggt ttcttctcta atgtggcagg
     9841 aaaacttgtg aatacagata atgtaacggc tgggatcgaa acagaagtcg gtaaaaaaca
     9901 agtagcagtt gatatggatg tcattgtgga atatggcaaa gatatcgaaa aaatctttga
     9961 agaaatgcaa gaagtaatcg gcaaagaagt aaaaaatatg acacatttag aagtgatcga
    10021 agtcaacgct aacgtagtgg acatcaaaac aaaagaagaa tttgaaaaag atcaagaaac
    10081 ggtgcaagac aaagtaactg acgcagcgaa aaaaaccggt gaatttgctt cgaatcaaac
    10141 agataaagta aaatcagcag ctggaagcgg cgcacaaaaa gtaaaagaaa atacagaacc
    10201 acgggttcag taataaggaa gtaaaacatt aggaggaacg aaacatggga tttatttggt
    10261 cacttatcgt cggaggaatt atcggagcaa ttgcaggagc aatcacgaat aaaggttctt
    10321 cgatggggat tattgcaaat gtcgtagcag gtttagttgg atcagcactt ggtcaagcat
    10381 tattaggttc ttggggacca agtattgcag gaatggcaat cgtgccatca ttgattggtg
    10441 ccatcatttt agtactaatc gtatcattct ttgctcgaaa actgtaaaaa gacttttaaa
    10501 aagaggtcgt gaaaaagctg ttcttcatca agtaataaga acagccaaac aaaaatagct
    10561 tctcatattt ttcatttggt tgggcatatt atcgaagatg caagcttttg aacactgttt
    10621 atacggaata agaggctgtg acaagacttt tgtcacagcc tcttatttat gcgggcaaat
    10681 tcccaattag atttttaaaa gtttttcctt aaatgtgtct ttattgatac agagaagctt
    10741 ctttaataaa acaatgcctc cctaaatttt cttgagatag gaaaggtgaa catacaaaat
    10801 aatacgacca ctttgaaaac ccagatgatc cacaaatgga tcgtatgata ctgatgaagt
    10861 aaaaagcaat ggatcagaaa ctcaaagaga ttgaaaaaga caaggaagaa ataatatctc
    10921 ttttttactc ttgtctgttt tagccaataa tagctcgtaa agtaatctag acaaacaaat
    10981 agaagtaaac aaagtttcgt aagagcagca atcgtatcat gttgctctgc tagacgattg
    11041 acgagcagga aaaatgttgg taatggaata tagatcacga gtaatgaaag aaaaaagtaa
    11101 acaataaatg gcatactatt cctcctaatt gtgtgagttc gccctcattt taaaataacg
    11161 atatttgttt gtgaagcttt ttcgtgtcgt aacaaatgtt atctaatgta tttttaaata
    11221 aataatcatt attatcaaaa taatg
//
LOCUS       DAZAFF010000090        11201 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000090 DAZAFF010000000
VERSION     DAZAFF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.091"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..708)
                     /locus_tag="ACG9BB_002324"
                     /pseudo
     CDS             complement(101..708)
                     /locus_tag="ACG9BB_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1231..1905
                     /locus_tag="ACG9BB_002325"
                     /pseudo
     CDS             1231..1905
                     /locus_tag="ACG9BB_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1898..2059
                     /locus_tag="ACG9BB_002326"
     CDS             1898..2059
                     /locus_tag="ACG9BB_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838220.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            2543..3139
                     /locus_tag="ACG9BB_002327"
     CDS             2543..3139
                     /locus_tag="ACG9BB_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFN2838221.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            3231..3566
                     /locus_tag="ACG9BB_002328"
     CDS             3231..3566
                     /locus_tag="ACG9BB_002328"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFN2838222.1"
                     /translation="MAAKYVNEEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKG
                     PFIKDGVPYLHCHHIEYLSQGGKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEK
                     RENSFSKIP"
     gene            3909..4232
                     /gene="relB"
                     /locus_tag="ACG9BB_002329"
     CDS             3909..4232
                     /gene="relB"
                     /locus_tag="ACG9BB_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFN2838223.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            4552..4779
                     /locus_tag="ACG9BB_002330"
     CDS             4552..4779
                     /locus_tag="ACG9BB_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFN2838224.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4795..5961
                     /gene="hemW"
                     /locus_tag="ACG9BB_002331"
     CDS             4795..5961
                     /gene="hemW"
                     /locus_tag="ACG9BB_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFN2838225.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6196..7239
                     /gene="hrcA"
                     /locus_tag="ACG9BB_002332"
     CDS             6196..7239
                     /gene="hrcA"
                     /locus_tag="ACG9BB_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFN2838226.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7333..7896
                     /gene="grpE"
                     /locus_tag="ACG9BB_002333"
     CDS             7333..7896
                     /gene="grpE"
                     /locus_tag="ACG9BB_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFN2838227.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7947..9776
                     /gene="dnaK"
                     /locus_tag="ACG9BB_002334"
     CDS             7947..9776
                     /gene="dnaK"
                     /locus_tag="ACG9BB_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFN2838228.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9927..11093
                     /gene="dnaJ"
                     /locus_tag="ACG9BB_002335"
     CDS             9927..11093
                     /gene="dnaJ"
                     /locus_tag="ACG9BB_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFN2838229.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         3945 a         1791 c         2288 g         3177 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaaaacaca gctaaaaatt
     2161 agttgtgttt tttattaggt ttacttttct ggtataaaag gaactagcta ctttttcaaa
     2221 atttctaaat cattcaatat gtagcgctta ctaaattatt cgccataaaa ttttataatt
     2281 ggatcactca ttgttttacc taagtcatat ttaagtaata accggtgttt ttacataaga
     2341 agacttcggg tattttttag tttgattagc tctgatatac ctataaagaa tatgctaagg
     2401 taagaatagt aaaaattcac atagatgagg tataataatg atatttttat atattacaga
     2461 taacttgaca caaaatgagt ttttttcgat ttttcaagtt atctaatgca attatattga
     2521 tatcattagg agctgcattg ttatgaatcc aacattgatt actaagaaag aattactaaa
     2581 aaaattagat atttctactg gtgtattagc taatttaatt agaaatggca tgccaaaaga
     2641 gggagaaatg tttaatcttg ataagattat tacatggcga gaaaattggt ctaaaaacat
     2701 attaggtgag ttagaagttg gtagagtata tactaacaaa gaaatttcag agaagtttaa
     2761 atgttccaaa cagggtggaa tgagaagatc tcatcaaact aatactcttg tactgtttag
     2821 tgatcaaaca ggaagtaatg tttataaaga taagtggtta aatggtattt tacaatatac
     2881 cggtatgggg ctaaaagggg atcaagtatt ggataagaat caaaataaag ttttagccaa
     2941 ttcaaaaagt aattttgtaa agattcattt atttgaaaca tttaaaccaa aagaacacac
     3001 atatctggga gaagtatatt tagcaggaca aatatatact gttaatgaaa aagatagttc
     3061 gggcaacagc agaaaagtat ataaatttcc attagcttta ataaatcaag agcaattgat
     3121 tgaagataaa gatatataac caggaagaaa atcaaactag acatattcgt aatttatcta
     3181 atgctaaatt agaggaagaa gcaagaaaag ttagtaatta taataaaaaa atggctgcaa
     3241 aatatgttaa cgaagaggag ttttgcttct caaaaacaaa aatatataaa cgtaatccct
     3301 tcgtatctga atatgttaaa cgacttgcaa aaggtatttg tcaactttgc caagaaaaag
     3361 ggccatttat aaaagacggt gtaccttatt tacattgtca tcatatagaa tatttgtcgc
     3421 aaggtggaaa agatgtgatt gaaaactgta tcgcgttatg tcccaattgt catgctagaa
     3481 ttcatgaact tgagttacct gacgataaag aaaaattgct gaagatcgtt gaaaaaagag
     3541 aaaatagctt ttctaaaata ccgtaattta tttttatagg acaaataagc taatgtgagc
     3601 tatgccttct atataagact aagttctatt ataagtaacg aagtaattct cgttccttcc
     3661 tgtagtaata ttttttaaat aacctatagg cttcttcaaa agtacatcct ccagtaaatt
     3721 ctaagtactg gaggattctt tttctagtgt tgctttactc atgaaaaagt agttggctac
     3781 ttcatcaaaa tttctgaact tttttaatgt ataataaaga gctcgatcat ttactacaag
     3841 gatgctagct cctacatttt cacagttttt tagcaagtaa atttttctaa aatcacatca
     3901 ggaaatgcat gagattcttt aagagaggca attatagggc tatgattacg gttcgtgtaa
     3961 gtgacgccaa aaaagaatgg ctttcttata tggctgaatt ttatgggatt actttatctg
     4021 aacttctaaa aaactattcg atggaacaac tagaagatga atacaatcgt caaacggctg
     4081 aaattgccca taaacattgg ataagaagtg ggaaaacaac agtattcatg gaagaatcct
     4141 taatgaattt ggtgggctgg aatgacctgc cgtttaaaat tcacgcccga aattcagaag
     4201 cagctctgat tggcaaatac gtgtattggt agagatagaa gataatcaat tcgttgttat
     4261 cgctattcaa gtagggcacc gaagaaatgt ctatgattaa gagactgttt atttttacaa
     4321 aaaaataata attggaatct agttaaaact cttgaagtga gagatttgac tagatttttt
     4381 tatgaataaa ataaggacaa aattgtttag gaagaatcat gagtgtaaaa aaatcatcca
     4441 agaagtcgat tcttggattt tttagtggat ttgttcgcca aatacgttta gtatgcgtat
     4501 atgagaggtt gtaagcaaag cgttgtggtc tgagcactta aggaggaatc tgtgaaaata
     4561 gcttttcata tttttgcaga ttttgactta atgccgaagg tcagctttgc gattaccgtt
     4621 tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca ataagcaagc atttttagag
     4681 ctaggtttcc ttgattctaa atatgcggct tatttcaatg agttggtgct tgaacgcgat
     4741 tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag gagaaagaat ttcaatgata
     4801 gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca ttcatatccc attttgcgag
     4861 catatctgct attactgtga ctttaataaa gtttttttag aagggcagcc agtagatgag
     4921 tatgtggaaa tgctgctgaa tgaaatgaga ttaatgctgg aaagtcatcc tacagcgaat
     4981 ttggaaacac tttatgtagg aggcggaaca ccaacttctt tatctgctaa acaacttgat
     5041 cgactattat caggtgctag agagatcctg ccttttcaaa atggtaatga gtttactgta
     5101 gaagcgaatc ctggtgattt gacccgtgaa aaacttcaag tgatgaaaaa ctatggcgtc
     5161 aatcgtttat ccatgggagt gcagacattt gacaatcgct tattgaaaaa aattggacgt
     5221 aaacatactg cagaagatgt gtatcaaaca atgcaatttt tagaagcaga aaattttacc
     5281 aatgtaagca tcgatttgat ttatgcatta ccgggacaaa cactagaagg atatcgggat
     5341 actttggaaa aagctttggc tttggatctg cctcactatt ctttgtactc attgatcctc
     5401 gaaaacaaaa caatgttcat gaactgggta cgacaaggaa gactcgaact gcctgatcag
     5461 gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa tggaaaagca tggacgtcac
     5521 caatatgaaa taagtaactt tgggctctcc ggacatgaat ctcaacataa cctcatgtac
     5581 tggaacaatg accactattt tgggtttgga gctggtgcta gcggttatct tggagatatt
     5641 cgttaccgca acaaaggacc aatccaacac tatttacgcc cattacgagc aaatgaacgc
     5701 ccggtattgg aagaagaaaa attgacacga aaaaatcaaa tagaagaaga aatgttctta
     5761 ggactaagaa aaaaaacagg gattactaaa aaacatttct atgatcgcta tcaaaaaaca
     5821 attgaatcgg tgtacggtga tgtgctaagg gaattagagc aagaagggtt acttgttaat
     5881 gaagaagacc gtgtttattt gacaccaaga gggacttttt tggggaacaa tgtgtttgag
     5941 cgctttttat ttgaaaaata gtttgaagaa tatttgtttt ttatcgaaac ttgaaaagaa
     6001 aaagtttaga aagtttgaca atgaggtatt gaagtattga tataactgga tttacatcaa
     6061 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
     6121 ggagaagggc ttcttgctat atttataagt gaattagcac ttagaatata tgagtgctaa
     6181 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
     6241 cactatacga gtagtggcgt gcccgttggt tccaaaacat taatgtctga gggagtcaag
     6301 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
     6361 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
     6421 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
     6481 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
     6541 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
     6601 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
     6661 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
     6721 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
     6781 cttcgtacgg agattccgat gattcttcat aaatatttcc agacgacaga agggatgtct
     6841 cagttatttg acaccgttct tggtcatgtt tttgaagaaa aggtttatgt cagtggacaa
     6901 atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct
     6961 tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag caatattcat
     7021 atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca
     7081 agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg
     7141 ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt
     7201 gctgattact accgctcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg
     7261 cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag
     7321 gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag
     7381 ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg
     7441 gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg
     7501 cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta
     7561 caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa
     7621 cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa
     7681 atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct
     7741 aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa
     7801 gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc
     7861 gtgttaagac caactatggt cattgttgca caataattgt gcggatataa aacttacact
     7921 ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga
     7981 ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag
     8041 gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag
     8101 ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg
     8161 gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag
     8221 caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa
     8281 aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg
     8341 ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc
     8401 ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg
     8461 gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa
     8521 cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg
     8581 tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac
     8641 gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa
     8701 tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga
     8761 cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac
     8821 gtcaagcgtt aaaagatgca ggtctgtcac aatcagaaat cgacgaagtt attttagtcg
     8881 gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac
     8941 caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg
     9001 tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta
     9061 tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa
     9121 gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat
     9181 tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag
     9241 atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa
     9301 acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga
     9361 tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtgaaa gatgcagaag
     9421 caaacgctga agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagacg
     9481 cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag
     9541 aagtgaaaaa agcagaaaca gctcgtgacg aattaaaagc cgctgttgaa gcaaacaaca
     9601 tcgaggaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta
     9661 aactatacga acaagctgca caacaacaag ctcaagaaaa tccagaagca gctcaaggcg
     9721 gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag
     9781 ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag
     9841 tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat
     9901 gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag
     9961 ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa
    10021 aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag
    10081 aagcatatga gattttaagt gacccgcaaa aacgcgctgc atacgaccaa tacgggcatg
    10141 caggcacaga tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg
    10201 gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag
    10261 gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg
    10321 acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag
    10381 aaatctgtca tacttgtggt ggaaacggtg caaaaccagg cacacaacca actacttgtc
    10441 ataaatgtca tggtgcaggt acgatcaatg tagaacgcca aacgccactt ggacgagtga
    10501 tgagccgtca aacttgtgat gtttgtcatg gcacagggaa agaaatcaaa gaaccttgtc
    10561 caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag
    10621 ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg
    10681 gcggaccatt tggtgatttg tatgtggttt ttcgtgtaga agaaagtgat atctttgatc
    10741 gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg
    10801 atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac
    10861 agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa
    10921 caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa
    10981 gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag
    11041 acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct
    11101 agtaaaaata taaagtagac ttgcccaaat tttctatttc tagtaaaaac gctaagtcat
    11161 taataattaa aaaccagacg aacaatcata cgttcgcctg g
//
LOCUS       DAZAFF010000091        11124 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000091 DAZAFF010000000
VERSION     DAZAFF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.092"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..1345
                     /gene="lysA"
                     /locus_tag="ACG9BB_002336"
     CDS             86..1345
                     /gene="lysA"
                     /locus_tag="ACG9BB_002336"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFN2838230.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1332..2132
                     /gene="dapF"
                     /locus_tag="ACG9BB_002337"
     CDS             1332..2132
                     /gene="dapF"
                     /locus_tag="ACG9BB_002337"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFN2838231.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2135..3016
                     /gene="dapA"
                     /locus_tag="ACG9BB_002338"
     CDS             2135..3016
                     /gene="dapA"
                     /locus_tag="ACG9BB_002338"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFN2838232.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3013..3729
                     /gene="dapB"
                     /locus_tag="ACG9BB_002339"
     CDS             3013..3729
                     /gene="dapB"
                     /locus_tag="ACG9BB_002339"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFN2838233.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3735..4430
                     /gene="dapD"
                     /locus_tag="ACG9BB_002340"
     CDS             3735..4430
                     /gene="dapD"
                     /locus_tag="ACG9BB_002340"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFN2838234.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4446..5447
                     /locus_tag="ACG9BB_002341"
     CDS             4446..5447
                     /locus_tag="ACG9BB_002341"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFN2838235.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5444..6805
                     /locus_tag="ACG9BB_002342"
     CDS             5444..6805
                     /locus_tag="ACG9BB_002342"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFN2838236.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6765..7916
                     /locus_tag="ACG9BB_002343"
     CDS             6765..7916
                     /locus_tag="ACG9BB_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFN2838237.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7891..9060
                     /locus_tag="ACG9BB_002344"
     CDS             7891..9060
                     /locus_tag="ACG9BB_002344"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN2838238.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9235..10614
                     /locus_tag="ACG9BB_002345"
     CDS             9235..10614
                     /locus_tag="ACG9BB_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN2838239.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10895..11124
                     /locus_tag="ACG9BB_002346"
                     /pseudo
     CDS             10895..11124
                     /locus_tag="ACG9BB_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3702 a         1945 c         2493 g         2984 t
ORIGIN      
        1 tttaatatta ttccaataaa attcaaatct ttatcaaatg aaattccaat aaaaacggaa
       61 aaaaagtgaa aagaggaatg aaaaaatgac agaattgata attggcggag tcacagcaag
      121 cacattggca gaaaagtata agacacctct gtatgtgtat gatgaagaaa agatgagaca
      181 aacgatgctg tcattcaagc gaggctttgt atcgaaacgt tttgatacaa aagtgcttta
      241 cgcctctaaa gcattccaaa cgattgagat gctgcattta gttcgtgaat atggattcgg
      301 gctagatgtt gtcagtggtg gggaaatcta taccgctttg caagctgatt ttcctagaga
      361 tactatctac ttccatggta acaacaagac gccagaggaa ctgatctatg cacttgaaaa
      421 tgatttgttg catattgttg ccgataattt gatggaagtt gaattattag cagaattaag
      481 ccaacgctat ttaaaaaaaa tctgtatcat gcttcggttg aatgtcggag tagaagcaca
      541 tactcatgaa tatatcgtca ctgcgcatat tgattcaaaa ttcggcatga gctatgaaag
      601 taaagagtgc caacaagccc ttcagttgat aaaagaaagc agctatctag agttagaagg
      661 atttcatgct catatcgggt cacaaatttt tgatgttact gcttggttag ctgaaatcga
      721 caaattgacg aattacttaa ccgattttga ccaacctctt tccttgaatc ttggcggagg
      781 gtttgggatt cgttatacac aaaatgatca gccgatccca gtcgaagagg cactaaaaaa
      841 actgattgaa tatacggaag aagctttgga aaaacgaaaa ctgaccattc gtcaattact
      901 gatcgaaccg ggtagaagta ttgttggaga agctggtaca acactataca cgattggttt
      961 catcaaaaag acaccacata aaaaatatta ctttgtagat ggcgggatga cagataatat
     1021 ccgtccagct ctgtatcagg cagaatacga ttgtgattta gcagcaaaac tgtcggaaga
     1081 aaaaacagag aaagtgacga ttgcaggaaa aatgtgtgaa tcaggcgatg tggtaattaa
     1141 agaagcttat ttgccgaaag cagaatcggg tgatctttta gctgtttatt ccacgggagc
     1201 ttatggatac tcgatgagca gcaactacaa tcgagcgaca cgaccagcag ttgtttttgt
     1261 caataatggc caatcaagac tgattgttcg cagacaagca ttttctgatc tactaagggg
     1321 ggaagtttcc tatgatcctt cataaatata atggctgcgg aaatgatttt atcttattag
     1381 attacgcaga agacacagat tatagtcaat tagccgcttc tttatgcaca aaagaaaaat
     1441 atgatataga cggattgatc gccgtaaaaa tcgatccgtt ggaaatgatc tattacaaca
     1501 aagatggcag ccgcgcgccg atgtgcggaa atggcattcg ctgttttgct cgatacgtaa
     1561 atgatcagaa catgatgaat gaacaggaat tcaacgtaga gacattagca ggcacaagaa
     1621 aaattacggt gttggatccc tcaccttttt attgcagcgt agatatggga gtgccagatg
     1681 attctccgga aaaaatcaaa gctaatcaaa cttatccaat aaaaaaccag gaaatagaga
     1741 tcaatggtga aaaagtaacg atcactagtt tatttttagg aacgattcat accgttgttt
     1801 ttgttgaaaa tgcgcgtgag gaagtctgga gaaaaagagg tgaactgatc tgtcatcatc
     1861 ctttgttcaa agaaaaaacc aatgtcaact ttgtagaagt agtcaatgaa caagaattga
     1921 tcgtccgtac ctatgaaaga ggagtaggtt ggacacttgc ttgcggtaca ggttgctgtg
     1981 cctcttatgt ggttgctaag gaaactggga aaatttcagc agatcaggta gttgttcact
     2041 tggaacaagg agacttgatg atctctggtg aggatgcgat acaaatggct ggcccagcag
     2101 ttcatgaatg gacaagaata ttggaggaat gaagatgata aaaggttcga tcgtggcgct
     2161 gatcacgccg atgaatgaag agggatcagt agattatgct ggtttggaaa aattgatcca
     2221 attccattta gatgaacaaa cagatggatt attagtttta ggtacaacag gagaatcttc
     2281 tacgctcaca caatctgagg aggagcagat ccttcaactg acagtaaaga aagtggctgg
     2341 ccgtgtgccg gtgattgcag gagctggaac gaataatacg aaagaaacga ttgaaaaagc
     2401 gaagcatttt gcttctttgg gggcagacgc acttttggtc atcacccctt actacaacaa
     2461 aacaagtgat gcaggactag ctgctcattt tacagcaatt gcagaagcct caccgattcc
     2521 gcttattttg tataatgttc cttctagaac aggcatgtcg attccaatcc atgtattagt
     2581 aaatttagca gaacatccta atatcattgg tttgaaagaa gcctcaggag atatggctta
     2641 tgtgatggat gccgctcggt tgattggaga agaattcttc ctttacagtg gaaatgatga
     2701 tttgatccta ccagtaatga gtgtaggagg aagcggtgta ataagtgtat gggcgaatat
     2761 ccaacctaaa atagtccatg aattagtaaa agatacgcag gacggaagat ggcagcaagc
     2821 aaaagaaaag caattaaatg cacttgaact gatccatgcc ttgttcagtg agaccaatcc
     2881 tattcctgtc aaagcagcca tgtcattatt agacttacct tccggacctc tacgcctgcc
     2941 tttagtttct ctttcaaaag agaaaaagaa acaattagct cagcttttgt taaaagaaag
     3001 gacggttgaa aaatgaaaat cggactgatt ggcagtggac agatgggaaa tagagtcgaa
     3061 gaagttgcac aagaaagagg agatacggtc cttcttttac ggacggcacc taaagaagca
     3121 actacgcttg cttttacgga actgccagaa ctgcttattg atttttctca tccagataat
     3181 ttagagaaaa tcctttcgta tagcttatca tatcaacttc cgttagtcat tggcacgaca
     3241 ggttatacac aatcacagtt ccaagaaata aaagaacatg ccaaacatgt acctgtgatg
     3301 tatagtgcta atttttcgtt cggcatcatg gtgatgaatc aactaatcaa gcaagcagct
     3361 gcaatgttgc aaggctggca gatcgaactg attgaaaaac atcatagtcg taaaaaagat
     3421 gcaccatctg gtactgcaaa tatgctgctt caaacaatcc aagaagtgca aaagctgcag
     3481 cccgtataca actggcaagg gaaaaaaaga gaagaaaacc aaattggtgt acacagtatt
     3541 cgtgcaggtt ctttacctgg tgaacatgat gtattgtttg ctgctactga tgaaatattt
     3601 acgatcaaac atgaatcttt ttctaatcga atctttgctg caggcgcagt agaagcagcg
     3661 gactggttga aagatcagtc accaggttat tacaaattag aagatttatt aatggacaaa
     3721 ggggtgtaaa aaacgtggaa gctcaagaaa tcatccgcta cattcaaacg gcatctaaaa
     3781 agacgcctgt gaaagtctat ctgaatgtaa ccgagccaat cgaatttaaa gaaagtaaag
     3841 tttttggtga aggaagcagc ttcgttgttt ttggtgacta tgagacgatt gctccggttt
     3901 tagaagctga atcagaaaaa atcattgaaa tcgaaatcga aactgctgca agatacagcg
     3961 cagttccttt gctagatatt aaaaaaatta atgctcgtat cgaaccaggt gctattatca
     4021 gagatcaagt gtcaatcggc aacaatgccg tgatcatgat gggagcgatt atcaatattg
     4081 gtgcagtaat cggtgaaaac acgatgattg atatgggtgc tgtcttaggt ggacgagcga
     4141 cagtgggcaa aaactgccat attggtgcag gagctgtctt agcaggtgtg atcgaaccag
     4201 catcggccaa accggttatc gtagaagacg gcgtactggt aggtgcgaat gcagtgattg
     4261 ttgaaggtgt ccatatcgga aaagatgcag tcgtggcagc aggagcagtg gttttagaag
     4321 atgtggcagc agaaactgtt gtcggtggaa tacctgcacg tgtattaaaa ataagtgatg
     4381 atcagacaaa agaaaataca gcattgattg ctgcgttacg cgaattatag aggagatgga
     4441 gaaaaatgcg cgaaatagat acctgtattg tgggggcaac cggattagtc ggccaaacga
     4501 tgctcaaagt gttagaagaa tatgattttc cagtaggacg attgaaatta ttagcttcaa
     4561 aacgttcagc cggtaaagtt tgtgtattca agaaaaagaa acacactgta caggaattga
     4621 cagaaacttc atttgaagga tatgatttag cgctattttc tgcaggtgct tctgtatcca
     4681 aagagtttgc gccaatcgca cgaagtcagg gactatttgt catagataat tcctcagcat
     4741 ggcgggaaga ttcggatatt ccattaatcg ttccagaggt caatctggaa gactatacat
     4801 tgaatcggct gattgcaaat ccaaattgtt caaccattca agcggtactt ccgcttaaag
     4861 ctttacaaga agcctttggt atccagcgag tcaattattc gacctatcaa gcagtttctg
     4921 gttccgggca aaaaggcatt gaagatcttc atcgtacgca aaatgacgag gaaccagttt
     4981 tgtatcctta taatattagc caaacggtga ttccggaaat cgatcagcct ttggaaaatg
     5041 ggtatacaaa agaagaacag aaaatgataa atgagacccg taagattctg catcagcctg
     5101 ctttgccagt atcagcaacc tgcgtacgtg tgccaatcga aaatggacat ggagtctctg
     5161 tggcagttca actgcaaaaa gagtttacga cggaagaaat tagagagtgt ctcagccagt
     5221 ttccagggat tcaattagaa gatgatctag cagcacataa atatccgacg agcattcttg
     5281 ctagaggaac agatatggtt tatgttggga ggatcagaaa agacacttct ttagagaacg
     5341 gattgttgtt ctatacgact gccgataaca tacgcaaagg agccgctgcg aatgccgtac
     5401 agattgctgc agctttaatg cagaaaatag cggaggtggc aagatgagta atacgctgaa
     5461 agtagcgaaa ttcggcggaa catcgatgtc tcatgaagag caattcaaaa aagtccgtac
     5521 aattgttctt tcagatccca atcgccaagt gatcgttgtt tctgctttag gcaaacgtac
     5581 gtcagatgat gacaaagtaa cagacttact ttatttgatt tatgcttacc ttcagcatca
     5641 tgttgattgg acaccccttt ggaaaaggat ttgtgatcgt ttcattcaag taagagatgt
     5701 tcttgccctg cagttaccta ttgaagatga actagcacgc atacagaaac aactggaaga
     5761 cgaaaagata actgaagatt atttagtaag tagaggtgaa tatctgactg ctcaactgat
     5821 ggctgagtat cttggttacc agtttattga cgcaaaagac ctgattttct ttgactatga
     5881 tgggaaaatc gatcaggaaa aaacagcaca ggaaatcaca aaagcattta aaagatatcc
     5941 gaaaatggtt gttccagggt tttacggggc gaaccctgca ggaaaagaaa aattattggg
     6001 ccgtggggga agcgacatta ccggcgcgat actggcgaag atgttgcaag cggaaagtta
     6061 tgagaattgg acggatgttt caggaatcat gatggctgat ccgcgaatca tcgatcatcc
     6121 taaaatcatc gaggaaatct cttttagaga actgagagag atggcttata tgggggctaa
     6181 cgttcttcat gaagcagctg ttttcccagt tcaagaggct gatatcccca tccaaataaa
     6241 aaataccaat gatcctttcg ctcaagggac aaaaatatcg aatcacgaaa tagaaaagga
     6301 aaatggactg acaggaatag ctggacgcaa ggattttctt tccatcactt tattcaaacg
     6361 tcatatgtcc gatgaaatcg ggtttatctg gaaagcaatg agcatttttg ctaaacatga
     6421 gatatcaatc gaacatattc cttcagggat cgacaatatc ggagtgatcg tctcagcaga
     6481 agcgattgcc gataagttat ttctcatcac gaaagaatta aaagaaacac ttggcgtgga
     6541 agaaatcgtc gtgatcgaag acttagcttt gatctctgtc gttgggggtc cgcataaaga
     6601 attgattgga ttatctggga aagtgttatc gatattgaac aaattagaaa ttcgcacatc
     6661 gatcttatca caaggagcac aggagttgaa tctgatcatc ggtgtaccga ataatcaata
     6721 tgaaacagta gtgaagggta tctatgaagg gatggtgaat acggatgctg aaagaatatc
     6781 gaatggcact ccatcaaatt cctgaaatcg ggtttcatga attcaagaca aaaaaatatt
     6841 tatatgaaca agtaaaaaac agcgaggccg ttatccatga aatcggcgaa acaggactac
     6901 tcctttattt tgataatcag caagaagaaa cgattgcttt tcgaacagat atcgatgcac
     6961 tgccagtcaa agaagaaacg aatctctctt ttgcttctaa gcatgaaggt tatatgcatg
     7021 catgtggtca tgacggacat atggcgatgc ttttagggtt gactgacttt ataactaaac
     7081 acattgagga tcttatttat aatgtagtac tgatctatca gccatcagaa gaaatttccg
     7141 gtggcgcaca aagcgttatt cagtcaggat tgttggataa atatgaagta caagcaatat
     7201 ttggctttca tctatggcct ggactagcta agggagaagt tttctctcga ccaggacctt
     7261 tgatggcaca aagcagtgaa acagacatta tcattaaagg ccagtctgca catattgctt
     7321 ctagtgaaaa aggaatcgat agtctggagg cagctgtacg atttttgaag aatgtttatg
     7381 attttgaccg gcaattaccg gcagagtgga atcatctaat gaaatttggc caaatccaag
     7441 gtggaacgat ccgaaatgtt ttagctggag aagttattgt atccggaagt atccgttctt
     7501 acagttcaga tatgcaaaat tatttgaaga ggggattaga ccatttagca gaacagtttc
     7561 actctacagc tactatttct tttcgctata atgacggcta tccagctgta atcaaccata
     7621 gaggcttata tgcagcactg ttagattcaa acaaactaca tgaattagat gctccagttc
     7681 ttcaagccga agattttggc atatatacgc aacaatatcc atgtgtattt ttctttttag
     7741 gtgtcggtga tacccctgct ttacatgatc cgacgttcga ttttgatatg gatgttttag
     7801 aaaatggtgt agaatggtac aaaacgattc tttttacaga aaatcttctt gtaaaaatcg
     7861 atagcagttc aaaagaggag cgagtaaata atgatgaatt tatccaatcg atttagtcct
     7921 aggctttcta gaatagaagt atcaaaaata cgacaattcg atcagcaaat atcgtcgata
     7981 ccagatgtga tcaaactcac cctgggagaa ccggattttc caactcctga acatgtcaag
     8041 caagcaggaa tagcagcgat tgaagaagac ttttctcact ataccggaat gagaggatta
     8101 gaggaactgc gggaagcggc ttgtatattt cagcaacagc gttatggatt aacttatgat
     8161 ccgcaaacgg aagttttgac gacagttggt gcaacggaag cgattgcctc tgcgttgtta
     8221 tctgttttgg aagaaggaga caaggttctt attccagcgc ctgcttattc aggatatcag
     8281 ccattagtgg aactagctgg tgctgaattg atcccgatag atacttctga cacagggttt
     8341 gtctgtcagc cggaacaatt tgaacgagca tttgaacaat atggatcagc tgtcaaagca
     8401 gtcattttaa actatcctaa caatcctact gggacgacat tgtctgcaaa acaattgaaa
     8461 gcaatcgctg aggttttaaa aaaatatccg gtatttgtta taagtgatga agtatatgct
     8521 gaattgactt atagtggaac gcatatgtcc atagctactt atcttccaga acaaacaatc
     8581 gtgataagcg ggttatccaa gtcacatgcg atgactggtt ggcgagtggg ctttattttt
     8641 gctcaaaaag cccttattga cgaactaatt aaagtccatc aatatctggt gactgccgct
     8701 acaacgatgt cgcaaaaagc tgcggctgaa gcgctaataa atggggtgaa agatagcgag
     8761 aatatgaaag agcaatatca aacacgaaga gactatctgg tccagcaatt ggcgccttta
     8821 ggatttgaag tgacccagcc aaacggtgca ttttacttgt tttgcaaact cccagctgcc
     8881 attcaaactg actcttggca gttttgtgta gaattagcgg aacaggcaaa agttgcgtgt
     8941 atcccaggaa ttgcatttgg cccagaagga gaaggttata tccggatttc ttatgctagc
     9001 agcatggaaa aattacatga ggcctgcaaa cggatcaaag actttttaac agaacagtga
     9061 ttcatagatt ttatcaaaaa aacaatcgtt aattttgcgg tatattctgc aaggagacga
     9121 ttgtttttta aatttagttc aaatgaacgc gtttcttttg attcataact aatgtaatgt
     9181 taagtttaaa ttgtttagaa tgattttaaa agaatattca aggaggtttc atcaatggaa
     9241 aaaatcaaag ttgtcattgt aggtgcatct catggagggc atcaatcgat cttggaacta
     9301 ttgacacgat acggtgaaaa tgtagatatc acgctatttg aagcaggaga ctatatttcc
     9361 ttcatgtcat gtggtatgga gctgtattta gaagacaagg taacagatgt aaatgatgta
     9421 cgtaatttca gaccagaaaa ctttccgcaa tccaatgttc atattttaaa taatcacgaa
     9481 gtaaaaacga tcgatgccga taaaaaaaca gtaacggtta ctagcgcatc agataacgaa
     9541 acaaaagaat atccttatga caaattgatt ttgagttctg gtgtcaaacc aaacaatctg
     9601 ccagtaccag gagcagatct agaaaatgtc tacttgatgc gtggatacaa ttgggcaacc
     9661 aaaatcaaag aaaaattaaa tgatccggct attaaaaaaa tcgctgtcgt aggtgcgggt
     9721 tacattggga tcgaagcagc agaagcaagc cgaaaagctg ggaaagaagt cgtattgctt
     9781 gatgtgatcg atagaccttt aggcacttat ttagatgctg agatgacaga tattttagaa
     9841 caacacctaa atgaaaacgg tattgaagtt ttgacaaatg caaaaatcga agcttttact
     9901 ggtaacggaa aagttgaggc aatcaaaaca agtgaaaaag aaatcccagc agatctagtc
     9961 attcaagctg ccggtgtgaa ggcaaataca gaatggttaa aagggatcgt cgacttagat
    10021 gaacgtggct ggatccaaac aaatgaatac ttgcaaacga atcttccaga tgtgtatgca
    10081 gtaggagatg ctacacttgc ttattctatc ccagcacgca aaaaaatgcc gatcgcttta
    10141 gcgactgttg cacgtagaga agcacgttat gtagtaaaac atctttttga agaagtacca
    10201 aacaaaccgt tcggcggagt cgtaggttcc tctgcattaa gtgtctttga ctatcatttt
    10261 gcagcaagcg gattgaatag ctttacagca gcaaaagcag gagtagatgt acgagtgtct
    10321 tattatgaag atacgttacg accaaaatat gtaccagaag aacaaggaaa tccaaaagtg
    10381 tccatccaat tattctcgga tccaatgacc catcaattgc taggaggagc agtattatct
    10441 acctatgatg ttacaggtca aggaaatgta ttggcactag cgatccaaaa caaactgact
    10501 ttagaagaac tagcagaagc tgacttcttc ttccagccag gctttgaccg acaatggagc
    10561 atgttgaatc tagccgctca acaagcgtta ggagaagaac catttgtaga ataagaaaac
    10621 gaaatatttg aaaagaggtt gtgaaaaagc tgccttgcat caagtaataa gaacagccaa
    10681 gtaaaaacag cttcttatgt ttttaacttg gctggactta ttaccgcaga tgcaagcttt
    10741 tgaacaccgt ttattttcaa aaaagggact gtgacacgat ttgtgtaccg acccccaaaa
    10801 gttagaccaa aaaatctaac ttttggaggt cggtattttt tatggttaaa tatacttttg
    10861 aacttaaatt aaagatcgtt catgattatt tggatggcaa aggcgattgg accgtaaaaa
    10921 tccagatcaa gagattgaag cggaaatgct taaaattcgg gagaagcaca aggattatgg
    10981 ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc tctatcaata aaaagaaagt
    11041 tcagcgcttg atcaagaagc taggaataaa agtaacggca tacacgcgta aatcacgtcg
    11101 ctacaattct tatcgaggaa aagt
//
LOCUS       DAZAFF010000092        11079 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000092 DAZAFF010000000
VERSION     DAZAFF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.093"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1086
                     /locus_tag="ACG9BB_002347"
     CDS             <1..1086
                     /locus_tag="ACG9BB_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838240.1"
                     /translation="LDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNWEGYENKNPY
                     VEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDKKSAETKP
                     LFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVYKNFVLND
                     LSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLSEKEQLLY
                     GKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVSESSVKQF
                     FYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGLETEKTKI
                     YPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1307..3070
                     /locus_tag="ACG9BB_002348"
     CDS             1307..3070
                     /locus_tag="ACG9BB_002348"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFN2838241.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3154..3612
                     /locus_tag="ACG9BB_002349"
     CDS             3154..3612
                     /locus_tag="ACG9BB_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFN2838242.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3721..4404
                     /locus_tag="ACG9BB_002350"
     CDS             3721..4404
                     /locus_tag="ACG9BB_002350"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFN2838243.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4551..5165
                     /locus_tag="ACG9BB_002351"
     CDS             4551..5165
                     /locus_tag="ACG9BB_002351"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFN2838244.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5181..5387
                     /locus_tag="ACG9BB_002352"
     CDS             5181..5387
                     /locus_tag="ACG9BB_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838245.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     regulatory      5612..5778
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            5926..6483
                     /locus_tag="ACG9BB_002353"
                     /pseudo
     CDS             5926..6483
                     /locus_tag="ACG9BB_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            6541..8580
                     /locus_tag="ACG9BB_002354"
     CDS             6541..8580
                     /locus_tag="ACG9BB_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFN2838246.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(8689..9840)
                     /locus_tag="ACG9BB_002355"
     CDS             complement(8689..9840)
                     /locus_tag="ACG9BB_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFN2838247.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(9994..10524)
                     /locus_tag="ACG9BB_002356"
     CDS             complement(9994..10524)
                     /locus_tag="ACG9BB_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFN2838248.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(10623..10973)
                     /locus_tag="ACG9BB_002357"
     CDS             complement(10623..10973)
                     /locus_tag="ACG9BB_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFN2838249.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         3992 a         1883 c         2164 g         3040 t
ORIGIN      
        1 ttggattttt ttaaagaaaa tgattatttg aaaaataaag aaatcattat cccagaattt
       61 gactcgcgga acatcttgct agctttagta cgttatgtca attgggaagg ctatgaaaat
      121 aaaaatccgt atgtagaaga agcttgtaac gagttgattg attatgtaga gactcatttt
      181 tttaaaagaa gatattcgga agatgaaaag tcctttatca tacgaggaat agagatcgca
      241 atcggaagaa aggcatttcc ccttggactg aaagaagaag ataagaaaag cgcagaaacg
      301 aagccgttat ttcaattagt ctgtcaagga ttggaagagc aaaaaggaat tctagactta
      361 caagaagaag atgtctatta tattttttct ttgttcaaca gtagaaatta taccaatgag
      421 aacatggagc tgttaagaaa agatttcgaa gttgtttata aaaattttgt tctcaatgac
      481 ctatcactga atgaattgat tttacagatt attagtatcg ctgaaatcga cgatgcagaa
      541 aattttattt tcaagcaatc ctttttacaa tttgtccgta cgttgtgggc agatggacaa
      601 gtatttttac cggaaaggat ctatcttcta tcagaaaaag aacaacttct gtacggaaaa
      661 attgtagaaa tattggaaga gtggaaaaag aaaaatcaaa tccaactaag atggaatgaa
      721 aatttcattc gaaaattcgt caaaagtctt tctttgatca acagtgaaca agcggggaga
      781 caggaaatag aaatttttgt tgtttcagaa agttcagtca aacaattttt ttatcgaacc
      841 caatttcatt tgtatgcgga agaaggcatc gctgaaataa atcctatttt atttcgttca
      901 ttagaagaat tgcctgatga atgtctatgt gcgaaaaaaa gagtggtttt gtgcgatgct
      961 gctagctatc aaaatggatt agaaactgaa aagacaaaga tctatccaat ctcattaaaa
     1021 ggtgcaaaca tccatttgta tcatattgct caggaacttc acctattaaa aaaacatatt
     1081 tcttgatgag accttaatta ggagaccttc tacaaagctt tttaattgtc tgtagaaggt
     1141 ttttatttga gtcctgattc gtgcgaagat ctacttatat aacaagcttt ctctgaaatt
     1201 tttttaaaat gctattgact tagagtcaac tctaagtagt agaatagata aagtgtaaca
     1261 aatctatttt cattaaaaga agatagtacg gaggagagaa tgtattatga gtacagtatt
     1321 acatgtattg gatgcaatca atagccccgc agatgtcaaa aaattatctg ttgaggaaat
     1381 gaaacagttg gcagaagaga tgcgccaaaa aatcttgcaa aaggacagcg atgtaggtgg
     1441 gcacgttggt ccgaatctag gtgtagtaga attaacgatt gcgtttcatt atgtatttga
     1501 ttcaccaaag gataaaatcg tttgggatgt ttcccatcag tcttatggtc ataagctttt
     1561 aactggtcga aaaaatggtt ttgaaaaagg acattaccgt gaagtgagcg gatattccaa
     1621 ccaacatgaa agtaaacatg actattttac agtggggcac acatctacat ctatcagctt
     1681 agcaacaggt ttagctaaag cgagagatct gaagaaagaa tcaggaaatg tggtagcatt
     1741 tatcggtgac ggctccctaa gtggtggctt agcttttgaa ggattgaaca atggtggtgt
     1801 tttacaatcc aatctaatcg tattagtaaa cgataatgag atgtctattg atgagaatca
     1861 tggcagttta tataaaaact tagctgagct tcgtcaaacc aacggtaaaa gcagcaataa
     1921 cctatttaaa gcaatgggat ttgattatcg ttacgtggat aatggaaacg atctggaaac
     1981 ggtgatccgt ttatttgaag aagtaaaaga tattgatcat ccgattgtac ttcacgtcca
     2041 tacggaaaaa ggtcgcggtt atcagccggc aatcgataat aaaatgaaat atcattggca
     2101 tgtaccattt gatctgtcca ccggggaggc aaagcaagta acttctggag aaacatttaa
     2161 tatgatcatc atggattatt tggataagaa aatgcaaaca agtacagctc ctattgccgc
     2221 agtcaatgca gcgataccag gcgcctttgg actgaaaaca ttccaagaaa aacatccaga
     2281 tcattatttt gacgtgggga tcgctgaaca gcactctatc actttcgctt ctgctttggc
     2341 ttctcaggga gtacgtccag tcgtcttcca atcaagtact ttcttgcaac gggcatatga
     2401 tcaattatca catgatttag cgatcaacga gaatccagtg attattttag tcggtggcgg
     2461 aacgatctca gctagtgacc gaacacacca aggaacattt gacattcctt acttatcaag
     2521 tatcccgaac atcaaatatc ttgctccagc aacaaaagag gatatgatct ctatgctaga
     2581 ttgggcgttc gaacaaacag cgcagcctgt agcaatccga attcctacac atggtgtaga
     2641 acatggtaca gctatcgcaa atgattatag tattccagcc tatcaaacag taaataatgg
     2701 aggaaaagta gcaattttag ctttaggcgg ttttcttgaa ttaggaaaag aaacagtaaa
     2761 agcttttaga aaaaaaggaa tcgaagcaac gttgatcaat ccaggaacga tcacagaaaa
     2821 tgacgtagaa acattagata aattaaaaga aacacacgaa ttagtcatca cattagaaga
     2881 tggtagtttg tcaggaggat ttggcgaaaa tatcagtcgt tattatggtg attcttcaat
     2941 gaacgtatta aattttggtg cagagaaaga attcacggat agcgttcctg tagggcagct
     3001 ttatgaacgt tatcatttaa caccaaaaca aatcgttgaa gatagtctgc gtgtcttgaa
     3061 tattcaataa ttcattatgc tcaccatcca gataattgtt tatactagta tctggatggt
     3121 tttttcatta taaaaagcaa aggggtggat gttatgtatc agcaagtaga agaatatgtc
     3181 aaaaagttcc aagaaaaata tcctagttat caatcagaaa aattaggttt cttttcattt
     3241 ggggatacag aaaaaatggc agacgagttg agcgaactag ttgtaaaaaa gataaagact
     3301 ggtacatcaa gtggttacga tctgtataaa caagagaatg agcctttacc aaaagttggt
     3361 caattagacg tggtcctgaa tggaaaaaat gaaccagttt gtatcatcaa aaatataaat
     3421 gtgaccatct tgccttttag tgaagtcacg aaagagcatg cttggaaaga aggagaaggg
     3481 gatcggacat tagcttattg gcggaaagtc catgctgagt tttttaaaca tgcttataaa
     3541 gaagcgttga acatagaatt tacagaagag aagctggtcg tatacgaaga atttgaagta
     3601 attttctttt agtaagaaag gtctgctgaa agaaatagct cattattgtc tcctgaatag
     3661 aaaagggtta catttttata tgaagctgta tttttatata acattactgg aggagatatt
     3721 gtggatacac tggaacaaat caaaggcgga gtcgtcgttt cttgtcaagc tttggaaaac
     3781 gagccgctac acagttcttt tattatgggg agaatggctt tggctgcaaa agaaggcgga
     3841 gcagttggta ttcgagcaaa tacgacagaa gatatcaatg aaatcaagcg aatcgttgat
     3901 ttaccggtaa tagggattat caaaagagat tacgatgggt cagaagtgtt tattactgcg
     3961 acagaaaaag aagtggaaga acttttggag acgcaagcag aaatcattgc actggatgca
     4021 acttctcgta aacgtccgac agaaaaaaca actgctgaac ttgtccaaat gattcacgaa
     4081 cacggtcggt tagctatggc agatatttcg acttttgagg aagcgcttca agcgcaagaa
     4141 gatggttttg atcttttgtc tacgacttta gcaggctata cagaatattc acgaaagacg
     4201 cagacacctg attttgattt attgaatcaa atcatagaac aagtcgatat cccagtaatt
     4261 atggaaggac atactgactc tcctgaacaa gtagagaaag catatgaatt aggtgctttt
     4321 tcagtcgtag ttggatctat tatcacccgt ccgcaattga tcactaaaag atatgtcgaa
     4381 gccgcaagta aagcaaataa ctaaaaaaca cgcacggcac agattcttga caaaaaagaa
     4441 aaattgatag atagtttaaa gcagattcta agaagatgaa aaaagcatta cttttcattt
     4501 tgtcttcaag atctgctttt taacgaattt tatagaaaga aggcgaaacc atgtttgaac
     4561 ggagtccttc agaattgaca gaacgtgaaa attacaagtt gctgattggt tcagtcatcc
     4621 ctagaccagt agcagtagta acgactaaat cagcaaaaga tatagtgaat atcgcaccct
     4681 tcagttactt caatatcgtc agttcgaatc gacccatctt atccttggcg atccagagaa
     4741 aacaagatgc agtaaaagac acagcaaaga atattcttga gacaagagaa gctgttatcc
     4801 atatccttga tgaggacaac gtagaagaag taaataaaac agcagccaat ctgcctaatg
     4861 aggaaagcga actgtacctg actgacttga ctttaactga ttcagtaatc atttcagtac
     4921 cagcattgac agaagcaaaa gttcgttttg aaacatcctt atatgaacat gtcgagatac
     4981 ggaataaaaa taaagtaaca gctgatttat ttttgctaga agtacagaaa tatcatttaa
     5041 atgaaacagt ctatgatgat aagacaggaa ggatcgatcc aagaaaattg aatgctgtaa
     5101 gccgtttggc aggaaatgat tatgcagcaa taggagaaat tttcacgatt gcacgtcccg
     5161 aataaaaagg agcactagtt atgttaaaaa taagctataa accatcgaca gattcaaaag
     5221 aaatgaaaaa agaatatgaa acagtgaatg attttctgca agggcagtac ttagaagttc
     5281 cgccattgca agaccatttt gtcgttacta ctgttacttt agatggtaag gaaatagaaa
     5341 tgccagatca aacgattagc ggattgttta attactttaa caaataaata tttattccga
     5401 aaagtgaaaa aacatataaa aaaacacata aaactaaaaa attatcaaag aatcctggtg
     5461 caactgaaag aatttgcaac gggattcttt caacttattt gttatttttt tctaaaaaat
     5521 attaaagatt gttaaatttc actatttcat ttcaaattcc attgcctaat cattaggaaa
     5581 agtctatcat gaactcgtaa atagaatata gtaataacct tgttaggtga ggctcctata
     5641 caaacatagg ctgttgccca gaaacgtcga aagtcgccaa tgggtaaaac agaaactgtt
     5701 ggattaaggc tttttccaat agctaagaat gttttcttta cgttgtatag tgctaaaact
     5761 cggacgatga gatgatgcta ggtcagaatt cttgttcgaa aaggtttctg accttttctt
     5821 tatatttttg tgactgactt atttgatgag acaaactcgc tcctcacctg acaagtggat
     5881 tgctgtgtga ggaggagatg cggtgaaaaa agatcgattg gtggaggtcg ctcggattca
     5941 cttctggttg cacaatacaa ccagaaaaca caacaagcaa aactgatctc tatcatgaga
     6001 gattcatatg tcgatattcc aggttacgga atgaataaga taaatgcagc ttactcttat
     6061 gggggagttg atttattaaa tcaaacatta aaggaaaatt tcaaatttga agctccctat
     6121 tatgcaagta tcacatttca agattttatc gattgcgtta atgaactgtt tcctggtgga
     6181 gtcaagatcg atgcagaaaa atctctagat ttagatggcg tatatataaa caaaggagag
     6241 caagtgatgg atggtaatac tttactccag tatgctcgat ttcgggaaga caaagaagga
     6301 gacttcggga ggatcagaag gcagcagcaa gtgatcaaag ctatctctca gcagctgaaa
     6361 gacgtaactt caatattcaa gctacccaaa gcggttggga aattacttgg tagtatccaa
     6421 acgaaccttc cagaaagtgt gcttctggat tgtggtatgg atttcctcaa agacatatgt
     6481 tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa ggaggaaaaa
     6541 atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc cggcgcagtg
     6601 atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca agaaacccaa
     6661 gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa caagggagat
     6721 tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc attatctgaa
     6781 aaagagatct tagatcaata ccagaatata tacggtgctg ctgatgtcaa aggacttcag
     6841 atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc atataaagca
     6901 aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac attagacaga
     6961 aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga aatggaagga
     7021 aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat agatcgaaat
     7081 ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc aagtaaactt
     7141 ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt cgacttaaca
     7201 gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta ctttgtccca
     7261 ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat tcaagaagta
     7321 gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta cgtgggagat
     7381 atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa aatcggtcgc
     7441 tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtga gaaattaagc
     7501 attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt tcaaaatggc
     7561 aaagatatca aattgacaat cgatgcgaag gcacaaaaaa cagcttttga cagtctagga
     7621 ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct tgcgcttgct
     7681 agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga agattacaaa
     7741 gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg ttatgctcct
     7801 ggatctacgt ttaaaataat tacagcagca atcggtctcg acaacggcac tatcgatcca
     7861 aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg gggatctttt
     7921 aaaacaacgc gtgtcagtag tgatgtatca caagtagact taaaaactgc tttgatctat
     7981 tccgataata tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa atttcgtaca
     8041 ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat gaatccagca
     8101 caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac tggatatgga
     8161 cagggcgaac ttctaattac tcctatccag caagcagcaa tgtattctgt ttttaccaac
     8221 aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga taagaagaat
     8281 gtcatcagcg aaacagcagt acaaacgatc gtgccagatc tgagagaagt tgtgcaagat
     8341 gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc gaaaactggt
     8401 acagcggaaa tcaaagaaaa acaggatgta aaggggcaag agaacagttt cttgtttgct
     8461 ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa agatgatgat
     8521 gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa ttatcaataa
     8581 aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt ccttttttca
     8641 aataaacggt tttcacgatg tctctttcac aacttctttt tgatttactc acacttcata
     8701 aacttttttt gtaaaatatt tgagaacaag tggagaaata agtgtactta ataaaatcac
     8761 gataactaaa ggtgaatagt actgtggttc gatcaattga ctttgctgtc caatttgtaa
     8821 aatgatcaaa gccatttctc cacgcgaaat cataccagca ccgaccatca atgaactatg
     8881 ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca agcaatttgg tcaaaatcgc
     8941 tacaagcgtc aatatagaaa taaagagtag ttgttcacta aagtcagaaa aatcaacttc
     9001 taatcctaca ctgacaaaaa atactggtat aaatactgca tatcctaaag cctctacatt
     9061 gtggtatact tcttctttaa cctttgtctg actgacagct atcccagcaa aaaaagcacc
     9121 aatgacagag ctaagcccaa caagatccgc tacgtaagac attcctaaac aaataactag
     9181 cgatacgatg ataatagaag aatttgcgta cactttttct gcaaatgaaa gtaggaaagg
     9241 cgcaatccat ttgactaata agaaaataaa aacaaaataa attagctgtt caatcaaaac
     9301 aagcggtaaa gaagcattgc tagaagcttc ccccgttaga aaagataggc tgaaacttag
     9361 aactaaaacg actaaaatgt catctaccac agaagcaccc aaaattgttg atccttcttt
     9421 tgtattcact acgtttagtt cttttaatac ttctactgaa atactgacag aagttgccgc
     9481 aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt tgcctgtcaa
     9541 ccagccaaaa acaaccggga agatgatccc aagcaaagca acgaacatac caggtcggaa
     9601 atattttttc aaaagagata aatcactttc cagaccagct aaaaacatta ataagaacac
     9661 accaatctcc gaaaagtcat ggactagaat atcaggtttg acccaattca atccagcttg
     9721 gcctagcaaa acaccaacaa gtaattgtcc aatcactgct gggataccta atctccgcga
     9781 gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa taaattccat
     9841 tctcatcatt cctttgtata aaatatagcc cgaacttagc ataacataca ttattgaaaa
     9901 aataggaaaa tatactcaca aaaaaaccaa caatccaaaa gatttttttg gattgttggt
     9961 ttttatacgg tctattttaa taactaaaaa tgattaacgt atagtcattt ttcttattct
    10021 gatgaaattg ataatctcct ggatgcccat gaaaatcaaa ataaagccga atatttggaa
    10081 tagaagcatc aaacttttga atggattgaa caataagacg aatccgccaa tcaacaacag
    10141 aatactacta taaacactcc aacttttagc ttttgtattc atgctgaaga acaactggaa
    10201 caagctgttg atgataacag ctaatccaag aagaatcggc aggatagaaa cgattgcagg
    10261 tgcaaaagca tagacgatca gagctaagat caggaaaaaa agaccactga acagaccgat
    10321 actccaagaa cctgttttcc ttttaaaacg gtaagcctca aaaagattca acaacccaag
    10381 caaaacaaga taggcggtga tcagataacc gataaaatga aaaacagcag aagggtttag
    10441 cacaatcgca atcccagaaa taatataaac agcagctctt aataaggcgt atttttgaat
    10501 attttgttta atcgtctcaa acatataaga aaacctcctt tgacttattt taacgtgaac
    10561 tagtcatttt accaaattaa atgttacaaa atgagcgaag gagaagaata tctctggaaa
    10621 aatcaacttc tgaggatctt ttccattttt ttgccttttg ctagctcatc gatcatttta
    10681 tctaaataac gaatttcttt catcaagtta tcctcgatgt cttctactcg tacaccacaa
    10741 acaacacctg taatcaagga acgtgaagga ttggggacag gggcttccgc aaaaaattgt
    10801 tcgaaagtca cttgtttttg tagttgatat tctagttctg tttgggaata acccgtcagc
    10861 caacaaatga tttcatctac ttcctctttt atacgatctt ttctctccgc ttttgctaca
    10921 tacataggat aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc cataatcagt
    10981 cacctttctt tttttcaaat tatttactac ttatagtata taagaaaaag agaagaaaaa
    11041 gcgattattc aaaaaaacaa aaaggattgt gacaaaatt
//
LOCUS       DAZAFF010000093        10926 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000093 DAZAFF010000000
VERSION     DAZAFF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.094"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            221..1456
                     /locus_tag="ACG9BB_002358"
     CDS             221..1456
                     /locus_tag="ACG9BB_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN2838250.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2455..2715)
                     /locus_tag="ACG9BB_002359"
     CDS             complement(2455..2715)
                     /locus_tag="ACG9BB_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFN2838251.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2742..4043)
                     /locus_tag="ACG9BB_002360"
     CDS             complement(2742..4043)
                     /locus_tag="ACG9BB_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN2838252.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4335..4793)
                     /locus_tag="ACG9BB_002361"
     CDS             complement(4335..4793)
                     /locus_tag="ACG9BB_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN2838253.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(4806..6137)
                     /locus_tag="ACG9BB_002362"
     CDS             complement(4806..6137)
                     /locus_tag="ACG9BB_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFN2838254.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6148..7590)
                     /locus_tag="ACG9BB_002363"
     CDS             complement(6148..7590)
                     /locus_tag="ACG9BB_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN2838255.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(7577..8341)
                     /locus_tag="ACG9BB_002364"
     CDS             complement(7577..8341)
                     /locus_tag="ACG9BB_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFN2838256.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8445..9320)
                     /locus_tag="ACG9BB_002365"
     CDS             complement(8445..9320)
                     /locus_tag="ACG9BB_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN2838257.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(9575..9659)
                     /locus_tag="ACG9BB_002366"
     tRNA            complement(9575..9659)
                     /locus_tag="ACG9BB_002366"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9623..9625),aa:Leu,seq:caa)
     gene            complement(9677..9748)
                     /locus_tag="ACG9BB_002367"
     tRNA            complement(9677..9748)
                     /locus_tag="ACG9BB_002367"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9714..9716),aa:Gln,seq:ttg)
     gene            complement(9758..9830)
                     /locus_tag="ACG9BB_002368"
     tRNA            complement(9758..9830)
                     /locus_tag="ACG9BB_002368"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9795..9797),aa:His,seq:gtg)
     gene            complement(9892..9976)
                     /locus_tag="ACG9BB_002369"
     tRNA            complement(9892..9976)
                     /locus_tag="ACG9BB_002369"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9940..9942),aa:Leu,seq:caa)
     gene            complement(9997..10067)
                     /locus_tag="ACG9BB_002370"
     tRNA            complement(9997..10067)
                     /locus_tag="ACG9BB_002370"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10033..10035),aa:Cys,seq:gca)
     gene            complement(10113..10184)
                     /locus_tag="ACG9BB_002371"
     tRNA            complement(10113..10184)
                     /locus_tag="ACG9BB_002371"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10150..10152),aa:Gln,seq:ttg)
     gene            complement(10196..10268)
                     /locus_tag="ACG9BB_002372"
     tRNA            complement(10196..10268)
                     /locus_tag="ACG9BB_002372"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10233..10235),aa:His,seq:gtg)
     gene            complement(10271..10344)
                     /locus_tag="ACG9BB_002373"
     tRNA            complement(10271..10344)
                     /locus_tag="ACG9BB_002373"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10310..10312),aa:Trp,seq:cca)
     gene            complement(10351..10431)
                     /locus_tag="ACG9BB_002374"
     tRNA            complement(10351..10431)
                     /locus_tag="ACG9BB_002374"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10395..10397),aa:Tyr,seq:gta)
     gene            complement(10433..10508)
                     /locus_tag="ACG9BB_002375"
     tRNA            complement(10433..10508)
                     /locus_tag="ACG9BB_002375"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10473..10475),aa:Phe,seq:gaa)
     gene            complement(10544..10616)
                     /locus_tag="ACG9BB_002376"
     tRNA            complement(10544..10616)
                     /locus_tag="ACG9BB_002376"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10581..10583),aa:Asp,seq:gtc)
     gene            complement(10626..10698)
                     /locus_tag="ACG9BB_002377"
     tRNA            complement(10626..10698)
                     /locus_tag="ACG9BB_002377"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10663..10665),aa:Val,seq:tac)
     gene            complement(10714..10785)
                     /locus_tag="ACG9BB_002378"
     tRNA            complement(10714..10785)
                     /locus_tag="ACG9BB_002378"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10750..10752),aa:Glu,seq:ttc)
     gene            complement(10800..10889)
                     /locus_tag="ACG9BB_002379"
     tRNA            complement(10800..10889)
                     /locus_tag="ACG9BB_002379"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10853..10855),aa:Ser,seq:gga)
BASE COUNT         3489 a         2346 c         1762 g         3329 t
ORIGIN      
        1 tcgtatttat ctggcacaac atataaatcg atcaaaaaat tgttgcgtgt ttttttgaaa
       61 attggagttt caacttgatt acaaaattta aatttagaag ttgtgaaaac agcgagagac
      121 agtttaatat tcaaaatata cataggctac tcgaacgctg tgttttttag tatactagta
      181 agaaacgaag ggatagactc attccaataa ggaggatttc atgaagacaa ttctttttgc
      241 tcctgcagta ttcaatttag ctgagacaac acgaatgatt gaggttgcca aagctatgaa
      301 agaccgttat tactgtgtat tctttggctt ttctaaaact tttgctcctt tgatcacaga
      361 aagtggcttt gagtatcatt tactagcgcc gatccttacg aaaaaacaag aagaacaaat
      421 cataaaattt gaccagttgc gtacacttaa aaaccctttt accaaagaaa tggttgcctc
      481 tcgtatcact agtgaacaga gtctgattgc taagtttgag cctcgactga ttgtgacagg
      541 ttcgaatgtg acaatttttt tatcggcccg caataaaaag attcctttag tatatgttaa
      601 accctatgct ttgagtgaac ctttttttca atcagagcat gcgctgatgc cccacgagtt
      661 acgtttcttc ggccctttaa gcaatcccct ttggcactgg acaaaaaagc gtatagtaaa
      721 tacccgatgg atccccaatg ccttcaaaca cgctgcacaa aaagaaatgc ttcctttacc
      781 aaaaacaagt ttacaaatga tggatgctga tctaaatctc atcacaacgc ctaaactatt
      841 tttggaagaa gaacagctgc cggaaaatta tcgaatcgtt ggtccaattt ttgctaagct
      901 cagtacttcc ttgcctgaag aagtgctaac ttttatacag cgaaagcaac aagaaagaaa
      961 acgtattatc tatttcgcaa tgggtagttc aggcaatccg cgtatgttac gcaaaatttt
     1021 gttcttctta caaaaacaac cggatctagc ggttattgca cctattgact atctgcttga
     1081 tgacattcaa tctgactcag aagatctttt aataacagaa tatctgtcta gtcatctttt
     1141 gcatgaattc attgattttt cttttatcca cggcggggaa gggaccgtcc aaactgcctg
     1201 tgaatccggc aaaccgttta tcggtgtggg gatgcaattt gagcaaagct gtaatattga
     1261 ctattgtgta aaatacggaa acgcaatcgc cctcacaaaa ccagtgactg aaagaaaact
     1321 gcagattgct ctgcgcgaag tttcttcacc caagattcgt cagcaggcac gcacgctgca
     1381 agccgttttt ccaataaatg gtccacaaca agcaattcaa gaaatcgaaa ccttcctgag
     1441 caaccaccca atatagtttc aagtagaaaa agctttttta atagagactc atcagtaatt
     1501 tcgctattcg agacccagac agtgaaaata caaaaaaagt aaatagtgga ttgaaaagtt
     1561 agagttactt aaattacggt gtctaaaaaa cacaaaatca tccttaaaat aaaagatctg
     1621 acgctatcca aatgagcata ggtagcgtct gccatttatt cttagtcaaa ttctttgaca
     1681 cctttctctg gttggatcat ctcaacgttt cttcaaaaga aagaccataa taattaggca
     1741 aatacatttt cttaaaatgt gtcatataga tctagaagca tcgctttgta gagatttgtg
     1801 ccgctagtcc cctcaacaat tactaaatca aatgtaggat ttaccacttt atctttgata
     1861 atcaacctcc ctcttctgtt gagacaatga tctcgcaatc cgatgcatac tcctatataa
     1921 actgccagca atccccacat attgagtaga tcatgtcaga atgttctatg tcgtcagcaa
     1981 cgatacacta aaagtcacgt tcaccatgac tgattgctat tcccagcaca attatttcta
     2041 tacacgtttc atgatttgaa taaacactca catcatacat attaccattc atacaccata
     2101 ctgcagcccc tactatatgg ttgtaagctc ccaaatctat atttgacttg atcactttgg
     2161 tagtagatct actaattcta aatccattgc tgataaaatt tccatttttt cctccttctt
     2221 tctgccaaaa cagttaaaac aataataatc tttatgtaaa aaagggctgt gacaaaagcc
     2281 ttgtcacagt cccatattcc gaataaacgg tggtcaaaag cttgcatctg cggtaataag
     2341 ttcaaccaaa cgaaaaataa gccaacattt cacaaaaatt taagaagcaa ttttcgtaaa
     2401 tttcttctta tttcttgaag ctaaccactt ctgttacaac cacttttaga acatttaaag
     2461 cttcgatgtg acaaaccatc tatgctgctc gatcataaaa ttgatagcaa aaattccaca
     2521 aagtacatat gtcccccatg aaaacaaaac attcatatgc ggtacaatga tctttggaag
     2581 aatcacgatc aaaaatgatg ccagtgccca aatccatcga ttgatttgaa tatgatatgt
     2641 agctagcata ttcaatataa ggtgactagc cgttaataaa aagataaaaa caaaaaataa
     2701 aatcagccaa tacattgttt cccaccttct aaaaaccaaa cttagacagt ctggacttcg
     2761 ttttctccac gactttgttc atctgacagc atctttttat cataagcttt tacaaatgga
     2821 taatagataa taaatgctat caatgtgttg atcatgatca agataacaac acgccagtca
     2881 tttcctgctg aaagataacc gccgattgga gctggtaatg tccatgttgg caaaacagta
     2941 aatctattga ccatttctaa tttgactgca aaataactga taatagtcaa aactaaagga
     3001 actaagttga acgggatgaa catgtaagga ttcaacatca taggagctcc aaaaattatg
     3061 ggctcattga tattgaagat accaggaata atagaaaaac gaccaacctc tttcaagaat
     3121 tttgatttac agaagaatat aaacaaaaca gataccacga tcgttgctcc tgcaccacca
     3181 attgttaccg tccattgata aaactgttca ggagcgatat agggtaattg ggtggcagct
     3241 gtaccatctg ccaatgcctt tgcattctca tccaacatga tcagccacat tggacgaaca
     3301 acagccccta caatactcat gccatggata ccaaatgacc acaacaaatg gatcaaaaac
     3361 attggtagta aaacaccaag caaattattc cctaaaatat tggttaaagg ctcgaataac
     3421 gataataagg cagcgtttac atcaaagccc gcgacgcttc gaatcagcca agtaattgtt
     3481 aaaatgactg cacctggaat caatgcctca aaactacggg caacagcagg tggcacttgt
     3541 tctggcattt tgatagtaat atttcgattt ttaaagaaac gatatacttc cactgcaaaa
     3601 atcatagata agatccccgc aaacatccca gatgctccca aagaactcat aggtaagacc
     3661 cagccaagag gtgcctcttc aggagttaaa acgtttacag gaacttgtaa cataaagaac
     3721 gttcctaaag ataatatacc acctgtcaca ctatctaatc catatgattt agctagatcc
     3781 gaacccataa caaaagttgc ataaatagcc atcaatccca tcgtcatcct aaagggtaaa
     3841 acaatcgttg ccgaataagg ggcaataaat tcattccatc caggaaacgg taaattgata
     3901 aagaccataa aaaatgtccc aatcaaaatc aatgacaatg ttgaaacaac cccattacga
     3961 attgctaata aatgacgctg tgtaccaatt tttgccattg gtgggacaag tttctcttcg
     4021 acccatttca tcagcttttc cactaaaacc actccttttc aataaaatat gaaccattca
     4081 ttgcatgaca acgcaaagaa tgttcataac taattatata attaagaaat tttatttcaa
     4141 tatattctct taaataatca aatttatttt taagaatcct gaaaataagt tttctaaatc
     4201 ataggagcaa caaaaaaatt cgataattaa caaaaatcaa aatgattaaa tctagtgatt
     4261 gaaacatatc atttcaaaaa aattttgtaa attcacccat aaatatcacc ctatttaact
     4321 tttttcatcg tacactaaaa ttcaaaacaa ctatctttaa tctttacttc ttggttttgt
     4381 tttaataaat ttccttcaaa aatgcctgga gaaactacaa tgatcaccgt atctttttgt
     4441 gcttgctcca acttcttcaa atctataata gctaacagtg tcccttcttt tacctcctgg
     4501 ttactttcaa cccgtatctg aaacggactt ccttcgagtt caactgtatc taaacccata
     4561 tggatcagta tttttctgcc agattcactt tcgatcgtta tggcatgtcg ggtaggaaat
     4621 atattatcga ttattccttt aactggcgca tggatatatc catcatgatt cgttatagcg
     4681 tatccaactc ctatcatttt tgaagaaaag acctcgtctt ttactgctgt taatggaata
     4741 atttgaccac tagctacagc ttttagaatg tttcttgttt taaagaaatg cactttttat
     4801 cacctctact ttaatgctgg ccaatatttt ttgtttactt tgatcatttc atctagtatt
     4861 ttttttgcga ctctggcatt tggtacggtt tgattcaatg taaatgcttg taaagctttt
     4921 tgataagaac cttcaaagta agcatctact agtaacttct ctactgcaac ttgtgcctcc
     4981 atcaatcctt tatgaaaatc actgattggt tttcttagtg taattggctc cacaccagtt
     5041 gcaccaacat atgccggtat ttcaacaact gcatctgacc gtagattcgg aatcgcccct
     5101 tgattaggaa cgattagcat aaagcgacga cgatcatcat tcaacaagga aatagctata
     5161 tcgacgatat attgtccatg ctccccaaaa ttaaagttca atacttcttc cacaataccg
     5221 cggcgtattt tatcagccat ttctttggta tttttctctc gtccatccat gaccatatta
     5281 gcacgtgtat actctttgtc agtatgctca acgaccatat caggatataa gtaatactct
     5341 aagtaattat tcgggatatt acttggaaaa tttttcgtga tgactgacat catttgaaag
     5401 gttttttgcc aagttttatc tcctatatta aaatcggcga cttgcatttc ttttgttatt
     5461 agtttttcaa taatctctgg cataatttct cgttgcaatt ctttatcata aatcgatgta
     5521 taccagccaa agtggtttaa accataatac gtaacgatcc aattcttacg atcataaccg
     5581 taattcactg caatcgtttc ttcaattgaa atagtcatat cacaggcatt aatcattttt
     5641 acattcggat atttacgacg aacagcttca gaaacgatcg actctggatt tgtataattc
     5701 aaaatccatg ctttgggggc atagtcttgt atatgatcga caatttccaa taaacctttg
     5761 attgagcgca tcccataagc aaatcctccg agtccacacg tttcttgtcc tactaatcca
     5821 tgtttaagtg gaatcttttc gtccttttct cgcatctcca atcctccaac acgaatttgt
     5881 gagaaaacaa aatcgcatcc tgtaaaagca agctttggat catccgttgc acgaatgcga
     5941 atatttgaaa aaccttttct tttcagcata aatttaacaa tcaagtacat atccatattt
     6001 ctttcttcat caatatcata taaacgtatt tcagataatg gcaaccgttt ttcattgttc
     6061 aaaatggctt gaacgattcc tggtgtatac gtactcccgc ctccagctat agtgataatc
     6121 tgttttttca tcatcatctt ttcctttcta ttcttctaat aatttattga attctgccat
     6181 aacataagtc acttctaagc cgatgataat ttgataattt ttttcagttg gtttaatgat
     6241 cccgcttatc cctaattgct tgattcgctg ttcctcgacc atggtgggat ccactacctc
     6301 tacccgtagg cgtgtattac aactatagac atccaggata ttggcttttc ctccaaacgc
     6361 ttgtagcatt tttgttgcca tatattcata atttttactt gctaacccta actctgtttc
     6421 ttctaaggtt acttcattgc caaactctgc cttagcttca cgtcctaatg tctgaatgtt
     6481 tctcttattg atcaaataat aaaaagtaac gtaatacaag gcaaaaaaga aaattccgat
     6541 tggaagaatc aaaatagcat tggtagcgat tcgaaaattg atgagataat caacaataca
     6601 tgctccgaat gtaaaaccaa ttcgtatatt gaataagtaa cagacaatcc ctgctaaacc
     6661 ggcaaaaagt gcatggatca cgtacaacat aggagaaaga aacatgaaac taaattcaac
     6721 aggctcagtg atatttgcaa taaatgaagt tcctgcaccg cttcccatga ttcctttgac
     6781 catctcttta ttttctttat aagctgcttt atagatagct agacatatag ctggtatacc
     6841 aaacatcatt actacaaaaa aaactgataa aaacagtcca gcctgaggat ctccgttgat
     6901 aaagcgtgtc atctctcctc tgaaaacttc cttgctagga gatgtatagc ttcctaaatc
     6961 gtaataaata tacgtattca acacatggtg cagtccaaag gggatcagca aacgattcaa
     7021 aaacataaat atccccacac ctaatgctcc catatcaacg agcagttttg caaaattatc
     7081 aatcccaatt tggatgagcg gccagataaa gcccaaaaaa acagctgcga ttgtttgtag
     7141 aatcatcacc atagtcaatg ggaatttttc gcccgcaaaa taagaaaaaa ctactggtaa
     7201 tttttgttct ttaaagcgat tatacgtcca tgccgctact aaaccggaaa taattccacc
     7261 aaaaatcccc atattcactt ctgggtccat aatttctaag ccttttttca aggtagcaat
     7321 cgctaaaaaa ccagtaagtg caggtattcc tttgtcctta tttttgacaa aaccgattgc
     7381 aactcctata gcaaataaga tatccaagtt tccaaaaacg gcatttccag ctgcaccaat
     7441 tactgggatg ttcagcatat cgtcagctga taaacgcatg atcaatcccg ctaatggcat
     7501 cgctgcaact ggaagtagca tcgctcttcc aagcttctgc acatttgatt taacattcat
     7561 ttttattcct cctctattaa atatcctcat atgttactaa ctcaatcaaa ggatcatttt
     7621 tcactttttg agcaaatgct gaatgaatta aaaattcgac ttggtgggta cgaggataca
     7681 caaaacttga accatttaac aattcctgat ccaaatacgc tgcgtgacac atgacttcga
     7741 tcgttttatc ttctctcaat atcatgagta aacttgccaa atattcttcc acactcgtat
     7801 tgatttgtcg ttttttttca tcagtttctc ctacgacatc gaaacaaggc tcaaaataat
     7861 cgacatgacg aacttcatct tttcgttcaa agttgctccg aaccggcaaa tcatattttt
     7921 ttgctaatgt aacaactacc tcctgatttt catccaaagt atgagtatga tgatgactgt
     7981 ctaaatgagt tggtatgatc cccgcatgat aaattttttg aatctgagca ttccattcct
     8041 gatacagttg atctttgtca atgttaaatg gatgctgata ataagataag gatctgaact
     8101 ctccgctttc gataagagta tcgacagttt tgaggagtgg tttgccacac gtcaatgtta
     8161 aatggactcc tacacctagc ctaggcattt cctttcttag tttcacagca tgctcgaaac
     8221 ccggcatatt tgccatcaat gtagttgatg tcaaaattcc tcgctgataa gcatccataa
     8281 tcccataatt cacaccatgg ctatagccaa aatcatccga attaaaaatc acttttccca
     8341 tttcattctc cttctttttt ctactaaaat aggcgcaacc aaataatcat agtccattta
     8401 ctatgtgtca tttggttgcg cctatagata gttacgccct tattttaata gattcttgct
     8461 agatgaattg ccaaatagat gatttcattt tcttgaatgg caagttgaaa ttgctgttcg
     8521 atgatatctt taatctttag tgctgtacaa tacgctttgc tatgtttaaa tgtttcagta
     8581 aaaaaaccaa tattttctac tggcgtttct tcctgataaa tcaagcgttt aaccaggaat
     8641 ttgatatgaa ccattaagcg atgataagtc agtgattctt tatcataaac gtttgaatag
     8701 gatttttcta gatgggacaa aatcgtggtc gtcatttcta ctatttttaa gctctttaaa
     8761 ctattaggct ctcctagctc agcattaatg atatgcatag tgatgaatcc tacttcattt
     8821 gaccctaact cggtttgata tcgatgattg attcgctcga ccacttctac tgctatatca
     8881 tattcatttg gataaaggaa tctcatttct ggcaacataa ttgctgtgac atctctatct
     8941 tctctttttc gttgaatagc aaaataaata tgatctgtca atgtaatata gatatttgaa
     9001 tctaatggag aggagatcct agctgttatc agttcaatca cttcttccgc aaaatccata
     9061 aattcgattg gaatctgcga gactatctca ctgaatcgtt tgatcttttc tttagaatct
     9121 aatacaaata atttgtcagc atcctcaaca tcaaatggct tactcacttt agcagaaaag
     9181 ccaatccctt tccccatcgc aattttttct attccatcgt catcgatcaa tacagcatta
     9241 tgattcagct ttttcatcac atacaccgtt aattcacctc ctctatatga taaaacaaaa
     9301 caacttgcag actccttcat acatattttt gtctccaaac aatttttaaa gacaaaaaac
     9361 gacctaagaa agaacccttt cttaggtcgt gcctaattga atagtagcac cctacataat
     9421 ttacaaaaat agtttaacac ataataaaag cgttatcaat atctttttta ctcgttttat
     9481 gataatactt gtatcgaatt gatttggagg taattacata aaaacaaaaa aaatatagca
     9541 actctctata agaaagttgc tatattgata attataccgg cggccggggt cgaaccggca
     9601 cgtcctcaac ggacactgga ttttgagtcc agcgcgtctg ccaattccgc cacgccggca
     9661 aatttaaaaa taataactgg ggtagctgga ttcgaaccaa cgcatgaggg agtcaaagtc
     9721 ccttgcctta ccgcttggct ataccccaag aataaagggc gaatgatggg aatcgaaccc
     9781 acgaatgccg gagccacaat ccggtgcgtt aaccacttcg ccacattcgc catcataaaa
     9841 gatgttattt aatttctcaa taaaaaacta agatgaagac atcttagtca ataccggcgg
     9901 ccggggtcga accggcacgc cctcaacggg cactggattt tgagtccagc gcgtctgcca
     9961 attccgccac gccggcaagt tgatttatta gtactaaggc ggtaaccgga tttgaaccgg
    10021 tgatgaaggt tttgcagacc tctgccttac cacttggcta taccgccatt atactattat
    10081 caatctataa aataaagaat aaattataga aactggggta gctggattcg aaccaacgca
    10141 tgagggagtc aaagtccctt gccttaccgc ttggctatac cccaagaata aaaagggcga
    10201 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    10261 acattcgcca tggcaggggc agcaggaatt gaacccacac taacggtttt ggagaccgct
    10321 gttctacctt taaactatgc ccctataaaa tggaggagag tggattcgaa ccactgaacc
    10381 ctaaggaacg gatttacagt ccgtcgcgtt tagccacttc gctactcctc catggtggcg
    10441 cgggacagaa tcgaactgcc gacacacgga gcttcaatcc gttgctctac caactgagct
    10501 accgcgccat attttaaaat tattaattga ataacaagaa cgacggttcc gacgggaatc
    10561 gaacccgcga tctcctgcgt gacaggcagg catgttaacc cctacaccac ggaaccagtt
    10621 cattatggag gttaacggga tcgaaccgct gaccctctgc ttgtaaggca gatgctctcc
    10681 cagctgagct aaacctccaa cattcaatta aaatgacccg tacgggattc gaacccgtgt
    10741 taccgccgtg aaagggcggt gtcttaaccg cttgaccaac gggccaggta aatttgctac
    10801 ggagagtaag ggattcgaac ccttgagaca gtttggcacc atctacatga tttccaatca
    10861 tgctccttcg gcctctcgga caactctcca gagtgaaaga agctcaatac tctttctcaa
    10921 aatgag
//
LOCUS       DAZAFF010000094        10714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000094 DAZAFF010000000
VERSION     DAZAFF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.095"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(232..1080)
                     /locus_tag="ACG9BB_002380"
     CDS             complement(232..1080)
                     /locus_tag="ACG9BB_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN2838258.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1244..2146
                     /gene="gnd"
                     /locus_tag="ACG9BB_002381"
     CDS             1244..2146
                     /gene="gnd"
                     /locus_tag="ACG9BB_002381"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFN2838259.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2148..3149
                     /locus_tag="ACG9BB_002382"
     CDS             2148..3149
                     /locus_tag="ACG9BB_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFN2838260.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3146..3430
                     /locus_tag="ACG9BB_002383"
     CDS             3146..3430
                     /locus_tag="ACG9BB_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFN2838261.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3463..4260
                     /locus_tag="ACG9BB_002384"
     CDS             3463..4260
                     /locus_tag="ACG9BB_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN2838262.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4274..5107
                     /locus_tag="ACG9BB_002385"
     CDS             4274..5107
                     /locus_tag="ACG9BB_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN2838263.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5125..5982
                     /locus_tag="ACG9BB_002386"
     CDS             5125..5982
                     /locus_tag="ACG9BB_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN2838264.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6004..6513
                     /locus_tag="ACG9BB_002387"
     CDS             6004..6513
                     /locus_tag="ACG9BB_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFN2838265.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6550..6951
                     /locus_tag="ACG9BB_002388"
     CDS             6550..6951
                     /locus_tag="ACG9BB_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838266.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7063..7416
                     /locus_tag="ACG9BB_002389"
                     /pseudo
     CDS             7063..7416
                     /locus_tag="ACG9BB_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7450..7893
                     /locus_tag="ACG9BB_002390"
     CDS             7450..7893
                     /locus_tag="ACG9BB_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2838267.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7910..8995
                     /gene="uxuA"
                     /locus_tag="ACG9BB_002391"
     CDS             7910..8995
                     /gene="uxuA"
                     /locus_tag="ACG9BB_002391"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN2838268.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8988..9701
                     /locus_tag="ACG9BB_002392"
     CDS             8988..9701
                     /locus_tag="ACG9BB_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN2838269.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9908..10057)
                     /locus_tag="ACG9BB_002393"
     CDS             complement(9908..10057)
                     /locus_tag="ACG9BB_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838270.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10453..10653
                     /locus_tag="ACG9BB_002394"
     CDS             10453..10653
                     /locus_tag="ACG9BB_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFN2838271.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3583 a         1694 c         2317 g         3120 t
ORIGIN      
        1 aaaaaaaaga aaagataaat tttttactac tgtatactaa ctttgttatc gcatctaaca
       61 agtaactcga ataataccaa tacaaccttc tccaaagatg cgatgacata cgttctaacc
      121 caccgactat tgatgttctg taccaccctt gcagaaccaa aaaagccact accaaatatt
      181 tggtagtggc tttttttgtt ttatggattt ttcgcttttt ctgttatggt gctattcttt
      241 gatttcagaa atagtatgtc gaactttagc gatagaacga cgtgattctt tttcgttgta
      301 aaacatcaaa atcacatata gagagtccaa aatgcttagt tgagaaatac gggaagctaa
      361 agcttcagat cggaattctg tttcttcaga aatagaaata aaaacgacat cgccaagttt
      421 agcaagggaa gaattagctt ggctagtaat aacgattgtt tttgctccag tttttcttac
      481 ggtttcagca atatggattg tttcttttga ttgccctgtg tgagaaatca aaacagcaca
      541 gtctttttcc gtcaataaag aagcttccat caattgaata tggtaatccg tgctgtgaga
      601 agttgcgata ggggaacgca aaaatttatg ataagcgtca gttgctacaa tctctgaacc
      661 gccaacaccg aaaaaataga cacgtttaga ttcattgata atagtagccg ctttttttag
      721 atccttttgt tgtaacaact tttttgtgtc atttaaagtt gaaatactag actcgaacac
      781 cttttgcgcc atagtgagct cagtatctgt tttctgaata ttttcgtgga tagaaatagc
      841 agatagatca ttttcttgga tcaacatggc cattttaaaa tctttaaatc cattataacc
      901 aagttttttt gtaaattgaa aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt
      961 gctaatagga ctatgactta ctttgcttgg attttctaaa atataatcgg cgatagcctg
     1021 ttcttttggg ctgaagtcgt tgtacattgt tttgatttgt agtcttatgg agacgctcat
     1081 gagaattcct cattttcata ataagattat cattttttcc gttctcatta atgttaaaga
     1141 tacaaggaga gtaggaaaaa atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa
     1201 aatttttttg tataatgagg gtataagaaa tggaggcgct atcatggaaa ttggtgtaat
     1261 tggtttggga aaaatgggat tgaatttagc tttaaactta aaagagcacc atcacaaagt
     1321 acaaggactc gacatttcaa gtacagctgt tagtcaggca agagaaaatg acattcctgc
     1381 ttatcaagag atctctgaat ttctagctac ttttgagcag aaaaaaatca tctggttaat
     1441 gcttcctgct ggagagataa ccgaaaacat gttaagaaaa ctttttccaa aactcggacc
     1501 aggagacatt attattgaag gcggaaactc ttattataaa gattcaatcc gtcgagcaga
     1561 agagtttcag aaaaatgaaa tcggttactt ggattgtggg acttctggag gaatcgaagg
     1621 agcacgacat caagcctgct taatgattgg aggagataaa gaaacttttc tatccgtgga
     1681 acaattattc aaagatgtag caattgaaag aggatatctg tatgctggtg catccggaag
     1741 cggacacttc ttgaaaatga tccataatgg aatcgaatat ggaatgatgc aagcaatcgg
     1801 tgaaggattc caacttgtac aaaaaagtga gtatgctttt gacttggaac aagtcgcacg
     1861 tgtttggaat catggttcgg tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt
     1921 aaacgaacat cccaatttaa gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa
     1981 atggacggtt gaaacagcgc tagaaatggc tgttccagtt ccagtaattg caactagttt
     2041 gttcatgagg aatctttcac aggaagaaga tagtttttca gcaaaagtag tttcagcact
     2101 aagaaatggg tttggcggac atgaaatagt tgaaaagggt gagtaaaatg ccagattatc
     2161 atatagcaat cgttaattcc agcagtttcg gaagagtctt tccacaccac ttggaaagat
     2221 taaagtcagt tggagacgtt catttcttta ctgttgatca agagattcca ggaaaagaac
     2281 tagcggagtt acttaaagga ttcaatatca ttattgcaag tgttacgcca ttttttcgac
     2341 gagatttttt tgaaaacaaa gatgaactgc tattgatttc ccgacatggt attggttaca
     2401 acaatattga tttaaaagca gcaaaagaac accagacaat cgtatctatc atacctgctt
     2461 taattgaacg aaatgctgta gcagaaaata atataactaa tttgttgtcc atgcttcgac
     2521 gcacgacaca agcacaagca agagtcaagg acgatcgatg ggaagatcgg gcggagttcg
     2581 tcggtaggac gctgtttaac aaaacagttg gtgtgattgg tgcaggaaat accggaagcg
     2641 gagtaatcga aattttgcgt aacggttttc gctgtgatgt tctagcttat gatccttaca
     2701 aatcagattt atacatcgaa agctttggtg caaaaaaagt agaacttcaa gaattactgg
     2761 aacaatcaga tatcatttgt ttatgtgcga atttgacaga agagaactat cacatgattt
     2821 ctgataaaga agtcgatcag atgaaagaaa atgtttatct atctaatagt gcaagaggcg
     2881 ctctagttga tgaagaagcg atgattaatg gtctgaaaag cggaaagatc gcaggatatg
     2941 ccacagatgt tttagaagaa gaaccgggac gtcaagatca tccgtattta gcgtttgaac
     3001 aagtagtgat tacgccacat atatcggcct acacaaggga atgtttagaa gcaatgggtg
     3061 aaaaatgtgt cagagatgtc gaacagatca gtcagcacct tcttcctgaa cgttctgtcc
     3121 agacaaagag caactatata aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa
     3181 aacaggccct caattttatt tatatgaaga aggtggtatt tcaaaagttt ctgatctctt
     3241 gaaatcatac ggcgcgaaaa gagtactggt cacccacgga acggtatcat gggaaaaggc
     3301 gctacctaaa ttagtatttt tgaatgacga aacgattcaa tttttttatc atcgttattc
     3361 tggagaatgt agttatgctg aagccagacg aatagctaca atcatcaaaa aaatgaaatc
     3421 gatttcgtag ttggcgttgg cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat
     3481 cttgcaagaa tcgatttcgg agtcatccct actttagcta gtaattgtgc tccatggaca
     3541 cctcttgctg tcatgtataa ggagaatgga caagcagaag ggaaaacaga acacttttac
     3601 agacaagctg ctttttttat aacagatccc caattagtga ttgattcccc tgttccatat
     3661 ttcattgccg ggttagcaga tacactagca aaatggtatg aatctgagct catcttagaa
     3721 caagattttt tgcaagatga atcatttttg aagctagcac aagatactgc gaagatctgc
     3781 aaagaagaga ttttgaatca ttctgctaaa gcaatccaag atatggcaca aagaaaactg
     3841 acatcggagt tcaaacagct ttcagagatt atctttgctg ttgctggttt agttggagga
     3901 ttaggagata aatacgcaag aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa
     3961 tatattccgg aaagccatcg ttttctacat ggagaaaaag tagcgtatgg gattttctat
     4021 caactggcac tagaaggtcg ctgggaaaaa atagacgagt tatatccttt gtatcgggaa
     4081 ttagaactgc cggtttctct agaacaaatg ggattatttc cgaaagataa acagataata
     4141 gataaaatgg ctgaatttat tgattcgaaa gagaaagtgc atttgattcc tatagaggta
     4201 acacagaaat cactgataca gtcaatgatg gaactggaag gttatatcag aaagatctga
     4261 aggaggagga cacatggaaa cattaagtgt agtacaaagt ttgttgattg ctttatgggt
     4321 agccgcggtt atgtcccgct ggttaggtgg aggagccacg ttgaccttac gtttttctcc
     4381 tttgatgact ggattagtcg tcgggttgat catgggggaa gttgcacaag cgatgattgt
     4441 tacagccgct ttgcaaatga tttatatggg ggttttttct ccaggaggat caatgccagc
     4501 tgaaccatct attgcagcag ctatcgcagt accagtcgca ttattagggg atctgtcgcc
     4561 agaagcagcg attgccgttg cggtgccagt cggattgctc ggaagttact tgtatcagtt
     4621 tcgttttttc atcaatactt ttttaggtaa atatacagac aaagctgtag aagatttgaa
     4681 tacaaaagct atggcccgat caattattct ttatccaacg gttgcctcat ttttattatt
     4741 cgttccgctt gttttcattg ctttatattt aggggcgcca ttgatcgctg atgtaatcaa
     4801 agctttagag ggaacagtcg ttatccacat tcttgaagta gtaggtggag ggttagcggc
     4861 aatcggtatc gcgacaactg tatacgttat cggtcgaaaa gattatctgg tctttttctt
     4921 ccttgcttat tttatgagtg ttgttttgaa atctttagat attacgatgg ttacttatgc
     4981 catttttggg attattatcg ctttgatctt tgtccagtcg caaaaaggga aacaagtaca
     5041 gcctgccaca agcaataaca attcagcatc ttttgatgac gaagatgact atgatgacgg
     5101 attttaattt aggaggaatg ggaaatggct gaaataatgg ataatcaaac aggtgctcct
     5161 gaagaaatca cgaaaaaaga tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa
     5221 attcctcact cttttgaacg ttacttggcg ccatcactac tatacggaat gatgccaatc
     5281 ttgaaaaaac tttataaaga tgaagactct ttaaaagcag catataaacg gcagttactg
     5341 ttcttcaaca ctcaattaag ctggggtggc ggtgtgatta ctggcctaat gtcttctatg
     5401 gaacagcaaa gagcagaaga agagtatgaa gaaaaagaga tcttgatgca ggatgacttg
     5461 atgtataaca cgaaagcggg attgatgggg gcactagcag gaatcggaga tgcaatcgat
     5521 tcaggaaccg tccaatatat ttttatcgca atcgcggtac cttgggcaca acagggaagt
     5581 cctttaggcg cactttttcc ttttatctgc tttgggttgt atcaggtttt actaggggta
     5641 tttttcgcaa gacaagcttt cactatgggc cgtaatgcta caggcctaat gcaaaacact
     5701 ggtgttcaga cagcgattga aatgctatca gttttagggt tgtttatgat gggggtgctt
     5761 gcgggaaact atgtaaaagt agaatcttca ttgaaatttg cgatatccgg acgtgagttt
     5821 gttatccagg aaattcttga tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg
     5881 ggtgtgtact tgttctatac gaaaaaagga ttgaaagtca cacaagcatt attatggctg
     5941 actggaatat taattgtgct agcaggaatc ggtattttat aaaaagaagg gaacgtgaat
     6001 aagatgggtg tagtcaattt agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg
     6061 gtaacgttat ctcaaaaaag tggagtgaat tccatttttg ttgtagacga agttgtagca
     6121 aaagataaat ttatgagaga tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca
     6181 attgtcatta caccagagaa agctaaattc tattgggatg aatatcaatt taaagaatac
     6241 aattgtattt tgatcgcaaa aacagtccaa acgatttacg acttagtaaa acacggaatc
     6301 cccatggaag aattgaatat tggagggatt gctcaaaaaa atcctgatac agatctttta
     6361 gtgacgaaat ctgtttattt aaataaatca gatgctgaaa aattgaaaga aatgaatgaa
     6421 caatatggcg taaagaatat ttattttcaa gccactccat ctgcacctaa aacaggatta
     6481 aaagatgtat tgaacaagtt tgatttgtca taaacaaatg cttagaaaaa agataaagag
     6541 ggcgaaaaaa tgaagaagaa cgagctgttt cgtgattggg aatttcgtta tcgctatatt
     6601 taccgtaagc gccgtacaaa aaagagtaaa cagcgttttc tttcagcatt agtatcagat
     6661 atttacagta tgagaacaga tgtgacagtc attgcttacg atacgctagc ctaccgatca
     6721 aaaaatattt atgttggaga tatcgaaaaa gcagagaaag ttatctgtac ttattacgat
     6781 acacccgttc atgcattagg aagttatttc atgtttgatt ggaaagacca aaggaaaaaa
     6841 acaatctact ctatattatt gtccttcatt ttgttgttca gtttaggttg gtggggaatg
     6901 atgatatata acaaaaatcc tcatcacgta ttcgatctgc tatccgtata aacaggtatc
     6961 actgtactag catttgggag ctactttttc ttattgggaa aagcagctcg cggatggcct
     7021 agcagacaaa cttttattcg aaatacttca tccatattaa cgatgctaga aatgatccgt
     7081 acgattgatg atccgagtgt agcgtatgcg tttgttgatg aaggctgtta tggagaaaag
     7141 ggcttagaca gcgtgagatc gggtatgaaa aaagaggcta ttctttttta tctagactct
     7201 gttggggcag atacaccgct acagttttcg ggaaattatt tttcaaataa agaacagtga
     7261 ctgaaacaag ttgataagtt gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa
     7321 aagcaagcgc agttcttcta tttgaccaaa acagatttac gagggaaaac atttaattgg
     7381 caaaatgcca atcagattat tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg
     7441 agaagactaa tgttggaaat catgatcgtt acacatggta agttgagcga aggactgaaa
     7501 gatgcggcgg aagtaattgt cgggacatcg agccatattg aaacaatgtg cttagagtct
     7561 ggggaggaca tccaaacatt cggagagaag gtcaaagaaa aattaactat gatcaacaca
     7621 gcagaaccca tgctagtatt tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc
     7681 ttggcagtca atgaactgac tgaagaaaag aaagcagcca cttatgtcat agggggcgtg
     7741 aatctgccaa tgttgttaga agcaatcaat catcagatgc ttcacacacc gatagaagaa
     7801 gcggtggaac aaattgtcaa acaaggaaaa gataatttaa ctgtttggca tgcttcagcg
     7861 gaagaaaaaa aattagatga ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg
     7921 atttagatgg tatggtgaaa aaggggattc gatcccatta aaatatgttc gccaaatacc
     7981 gggaataaat gggataattg gaactttgct gactaaaatg cctggagatg tttgggagat
     8041 ttcagaaatt gaggagttga aagcatctgt tgagagccaa ggactggaac tgcttggaat
     8101 tgaaagtgta gcgatccacg atgcaatcaa ggcagggaca gatgaacgag attactatat
     8161 tgataactat atacgaacaa tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta
     8221 tagcttcaaa ccaatttttg gatgggcgaa aactgatttg tttaatgaaa acaacgatgg
     8281 aagctattct cttttatacg atcagaaaat cgtcgatcag atggagccag gagaaatgta
     8341 tcaattgata cacagccagt caaagggatt caaattgccg ggttgggaag aagaacggtt
     8401 gaaaaaattc gctcagctgg aaaaactgta tgaaggaatg accgaagaag aattatttga
     8461 aaatctgact tattttttag aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat
     8521 ggctatccat ccagatgatc cgccatggga actttttggt tttcctagag tgacgaaaaa
     8581 tcttggcgac ttgaagaaaa ttctaaatgc agtagattct ccatataatg gactaacgct
     8641 gtgcacagga tctttagggg caaatccgga aaacgattta gttgaaatca tccatgaggt
     8701 aggttcgcgc atccattttg tccacttcag aaacgtggag tttttaggag aaagaaagtt
     8761 caaagaaacg gcacatccct ctacagaagg ctcccttgat atgtatcata ttatgaaagc
     8821 gttggtgcaa gaagggttcg acggtcctat acgccctgat catggtagaa ccgtgtgggg
     8881 tgaagtggct atgcctggat atggcctgta tgatcgtgca atgggactca cttacatgca
     8941 aggtctatat gaggcgataa gtaaggaaaa accactggga gagcattatg aataaaatca
     9001 agctcttcat ctttgatatg gatggccttt tattagaaac tggacgttta gcttatcgtg
     9061 cttatgtaaa atcagcacaa aagtatgatt atgaaatgcg taaggaagtc tattatttac
     9121 taactggcca aacagaaatg gcgataagag aacaaatggg cattttgtac ggagaagatg
     9181 ttccatatat caaatggcga gaagcaatca atcaatataa agaaaaaatc gtgaaagaag
     9241 ataagagagt atatacgaaa aaaggagcag aagaaatttt atcttttgca aaagaaagag
     9301 gaatccatac aatcgtggct tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa
     9361 tggaaaatct atatgccttg tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa
     9421 agccggaacc agagatcttt ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag
     9481 cacttgtatt tgaagactcg attgcaggga tcgaagcagc aaggcgagcg ggcattttgt
     9541 cttttctcat caaagatcat ttgatagggt tgcccgatca taatgggaag cataagttga
     9601 aagtagatgt aagtttatat gaatccaata aagcagacta tgaattttat gacctgcatc
     9661 aagcaaaacg atttttagaa agtaaagggt tgtatctcta ataaagagga ctattttcta
     9721 gtcctcttta tttattgtcc tgcacgactg agggaacaga tgaaaaagaa gtaatttttt
     9781 attttgaagt atctacctta tttgtagtga acaatttaga acaaacacaa aacgcatcta
     9841 ctgttatcta ccagtagatg cgtttaaaga gaagaacgag aatcgtccat atgagggttt
     9901 ccactcctta tgcagatttt tttaattctt gcactgcttg ttcgatcgta acacctgtcg
     9961 ctacatgatt ggtattttct ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg
    10021 ttactcgata ttgtatatct ttgttataga atgtcatagc cattctcctt tctttaaata
    10081 cttgctgaaa ggcaacgaac gcgttgataa cgaaattcca tttataagta taccaccaat
    10141 tgaaaggttt tcatagttaa gaaattttaa agtcaaaagt tgcagataaa agcaaataaa
    10201 caagaaaaca aaaagtgaat caatcgcggt tattcaactt tttcttcgat tcgaagacaa
    10261 tagaagacaa tgaattttat atttcattgc attatccttg tgttattaga aaaagcatga
    10321 tatattaaat gagtaatcta ttttgaaacg taatttgagc gatatatcta catagataaa
    10381 actccttttg cctatcaaat atttattgca acgaaaaaac gtacattgat acgtatcagg
    10441 aggaatttat ttatgaataa cggtacagta aaatggttca acgcagacaa aggttttggc
    10501 tttatcactg gtgaagatgg aaatgatgta tttgcacatt tttcagctat ccaaggtgac
    10561 ggcttcaaaa ctttagaaga aggccaagca gtgtcttacg atgttgaaga tggtcaacgt
    10621 ggccctcaag caacaaacat tgttaaagct taataaaaat tgacttttaa ccgtcttttc
    10681 gaaagaaaag acggtttttt tgtacgaaaa aatt
//
LOCUS       DAZAFF010000095        10566 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000095 DAZAFF010000000
VERSION     DAZAFF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.096"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG9BB_002395"
     CDS             33..656
                     /locus_tag="ACG9BB_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838272.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG9BB_002396"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG9BB_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFN2838273.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG9BB_002397"
     CDS             2152..3033
                     /locus_tag="ACG9BB_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2838274.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG9BB_002398"
     CDS             3036..3806
                     /locus_tag="ACG9BB_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2838275.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG9BB_002399"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG9BB_002399"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFN2838276.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG9BB_002400"
     CDS             5117..6280
                     /locus_tag="ACG9BB_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN2838277.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG9BB_002401"
     CDS             6397..6807
                     /locus_tag="ACG9BB_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN2838278.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG9BB_002402"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG9BB_002402"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFN2838279.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACG9BB_002403"
     CDS             9380..9664
                     /locus_tag="ACG9BB_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838280.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACG9BB_002404"
     CDS             9824..10060
                     /locus_tag="ACG9BB_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN2838281.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACG9BB_002405"
     CDS             10057..10455
                     /locus_tag="ACG9BB_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFN2838282.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3688 a         1770 c         2232 g         2876 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatat
//
LOCUS       DAZAFF010000096        10180 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000096 DAZAFF010000000
VERSION     DAZAFF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.097"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..506)
                     /locus_tag="ACG9BB_002406"
     CDS             complement(<1..506)
                     /locus_tag="ACG9BB_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFN2838283.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNL"
     gene            1200..1586
                     /locus_tag="ACG9BB_002407"
     CDS             1200..1586
                     /locus_tag="ACG9BB_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN2838284.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1680..1871
                     /locus_tag="ACG9BB_002408"
     CDS             1680..1871
                     /locus_tag="ACG9BB_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2838285.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2385..2717
                     /locus_tag="ACG9BB_002409"
     CDS             2385..2717
                     /locus_tag="ACG9BB_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN2838286.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2627..2821
                     /locus_tag="ACG9BB_002410"
     CDS             2627..2821
                     /locus_tag="ACG9BB_002410"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN2838287.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            2860..3006
                     /locus_tag="ACG9BB_002411"
     CDS             2860..3006
                     /locus_tag="ACG9BB_002411"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838288.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            3444..4784
                     /locus_tag="ACG9BB_002412"
                     /pseudo
     CDS             3444..4784
                     /locus_tag="ACG9BB_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5025..6641)
                     /locus_tag="ACG9BB_002413"
     CDS             complement(5025..6641)
                     /locus_tag="ACG9BB_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN2838289.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6759..6977
                     /locus_tag="ACG9BB_002414"
     CDS             6759..6977
                     /locus_tag="ACG9BB_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2838290.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            7172..7633
                     /locus_tag="ACG9BB_002415"
     CDS             7172..7633
                     /locus_tag="ACG9BB_002415"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN2838291.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7826..8065)
                     /locus_tag="ACG9BB_002416"
     CDS             complement(7826..8065)
                     /locus_tag="ACG9BB_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2838292.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(8089..9612)
                     /locus_tag="ACG9BB_002417"
     CDS             complement(8089..9612)
                     /locus_tag="ACG9BB_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFN2838293.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9630..9752)
                     /locus_tag="ACG9BB_002418"
     CDS             complement(9630..9752)
                     /locus_tag="ACG9BB_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFN2838294.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9782..>10180)
                     /locus_tag="ACG9BB_002419"
     CDS             complement(9782..>10180)
                     /locus_tag="ACG9BB_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN2838295.1"
                     /translation="KDFYNLKKFNHPLVFESDSSENHYNGKYYHQHHQVHEFKSILLT
                     SDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQDFIVAMQEVNEQVNFQYTTRQ
                     KDQTTIILIGKMLDEERIKQSFDKLLAESK"
BASE COUNT         3337 a         1909 c         1586 g         3348 t
ORIGIN      
        1 agattatcta ttttgatttg ctcgatagtt ttacttagat tttctgtata ttcaattaac
       61 ggttcatgga atagatcttc tatttttcca gtaaaaaaat aatgaactaa aatcagaata
      121 tctcgaatgt ctcctaaaga atacaaagtc aaattttgca atgaattata gagtcgttct
      181 ctatcagcca ttgcaacatt caaattgttc atttttagtt taaaaaaaac tttatcaata
      241 ttatttgctc taaatggacc aaataagtaa tatctggttt taaacatgta tagaagaaac
      301 atctcattaa aatcaccagt aaagaaatgg aaatctttag tttttttaat attatcagtt
      361 agtaactttt tataatcata aaaaaaataa gtagttctat ctgagcgata ctcttttata
      421 atactgaatt cggaattaaa aaccacgata ggtaacttta aacaaacatg aagagacttt
      481 aataagactt ctttatctga aaacactttt gtttctctct ttcctttcta catcattaaa
      541 tagtatttta tacgttatct aaaatactat tcctctgtca aaataagcta acttacttca
      601 ttataaaata tccacttatc gctttcatgt atattccacc gtaaaaagta cattttatca
      661 ccgagaactc atcatttttc atcatgatga atactattta catatatcta gttcaaagac
      721 aataattatt atgatccgtt attatctaag cactatacag ataataggaa tttttcgttt
      781 ctaaatattc caatttggga atccaataaa aattttcgtt caaatacgtg tctattcgaa
      841 ctcttttcta gttgttaatt atgaaggggg aagaaataaa aaatacaatc tatacacgaa
      901 taagaatatt atccaaaagg aggttttaga aaagatttat tatacgtaaa aagctcaaga
      961 cattttctta gccataagga catgtttgat atttaagaag atatatgaat tttcttttct
     1021 aagtataaag ataactatcc attatcaact aaaaaaacat ttttgaaaat aaacatcagc
     1081 agtcctatca atgcatgtga gcttatagaa gttattcgca gcgcagcata attgcattag
     1141 cttcttttcg tgttttttgc ctcttttccc tttaaatctt aatggaggag gtgtgcaata
     1201 tgtcagataa cgacgaacgc gtaaatgccc agttgattcg attctttgaa aaggtcattt
     1261 tgaacaccgc taatacttat tataaaaaac aagaagaaat tcaaaaacat gaacaacttg
     1321 atcaactccc accatatttg tttactaaag atactataaa atttaaacaa ccgattaatg
     1381 ttttaaatgt gactctcctt gttgaagata taaaattagc aaaaatcctt cctttgttga
     1441 aagaacctga acaaattttt cttatcgaaa aatttatctt tgataaaact gataaagaaa
     1501 ttggaaaggt tctggggatt acaagacagg gtgcgacaaa tttaaaacat cgactttaca
     1561 aaaaaattca aaaaatgctg gtttaatatc tgcataataa ctaattttgt atgcttcttt
     1621 gctcgttctc ctccttccca aagaaataga ttataagtcc atgggaagaa ggtagattta
     1681 tgaattcttt aaaagaagat ttcattttag caaaggctgg aaatgaagaa gctgttgagg
     1741 caattctcaa aagatttagc tctttgattc ataagcaatc ctggagaact ggaaaatatg
     1801 atcaagattg ttatcaagaa tgtatgcttg caatatatct agctatctcg aaattcgaaa
     1861 ttaaagaata actcaactaa gggagtctgt gacataagta aaaagatagt ttcaaatttc
     1921 gaacaataaa tttgaaacta tcttctttat aattataaag tatcgatttt acagcgttct
     1981 ataatgattc atagaatcta aaacgaatga ttttaattat caacgttttt taattcgttt
     2041 ttagcttaaa aataactttt gtcacagtct cactcctaaa attactttat ttacgtatga
     2101 agtgggcagg aggtcgaatc cagaactggg ctatggtact tcaacgagga cttctcctct
     2161 agaattgaaa agcatgcgat ttaccttccg taacgacggt agatttattc aactatgtgt
     2221 ccaatcaaaa tttgtctgat ttcaaaaata gtcaagagaa ttttggttgt taaaaactct
     2281 tatctatcaa cttccacagt ttagttgaca aaccctgata ctaagttggg ctatgataca
     2341 cttttcttga ctatcagtta cgttaccgga aggaatgaac tctattgaga aaaacatatg
     2401 atcgtaaatt taaactaaag atttcacagg atatccttga gaaaaaagta actaccaaga
     2461 aaattgctga agaatacaat atctctcgtc caacaatttc ccgatgggtt tcagaatatc
     2521 gtcgatacgg gaaaaatgct tttgctggac aaggaaaaag attacctgat aaagcagatt
     2581 tctacattct tgaacaggaa aataaacgac taaaagagga agacgagtgt tctacgaaca
     2641 caaagatcga tacggatctc cagggatcac tcaaaaactt tacgatgaag gaatcgaaat
     2701 caataaacgt gttgtagctg ttcttatgcg tgaaatgaat ttatgcacaa gaggatttca
     2761 tcgacgcaaa tcttctcatg gtagagaaaa aacaatcaaa gaaatggtaa aaaaatgttg
     2821 aatcgataat ttgaacagaa ccaaattgat gctgtttggg tgatggatat tacttacatt
     2881 ccatgtaatg acggtcgcct ctatttgtcc acctatattg acttagcaac tcgtattcta
     2941 cgttactata tggtagattc acacatgaaa aaagagattg tcgtccgtcc attacaaatt
     3001 tattaaggag aattgcctac ggtaattcat tccgatagag gtagccagca tcggtctttt
     3061 atttatcaag agctgttgaa cgaccaccac atcacacatt cagtgagcca aactgggact
     3121 cctgttgaca atgcagtaat tgaagcgttt catcgatcaa tcaaatgaga attaatcgac
     3181 tccaacaaat ataaaagtaa aatagagatg aaggtgttaa tccaagatta tttagaagac
     3241 tactacccaa acaaaagaat ccatacaaaa tttatgatga ctccttgaca atttgaagcg
     3301 aagaaaataa gggatctata atatctagtg ttggtgacgc cttctgttgc caatactttt
     3361 tcttttttct aataaacgca aacagacacc ttaaaatcct ttagaataaa gtcgacgaaa
     3421 accattcaaa ggagtgtttc aagatgtcct attcagagtt taccaaagag ttactagata
     3481 ttctagacct aaatcttatc tttcatgaag atgcctttcg caaagagcga atcaatgacg
     3541 aaacatgttt tgtttttgat ggcacattga cctatcaacc ggaggaatgc tttcatcgcc
     3601 actatcaaaa tgaacaaacg atcattaaat agggctggaa aaaagtatcc atcttattga
     3661 atgatgtcag taactacaag accattctgc ggattaacaa acagcgcttc aaatgtaaac
     3721 actgtggaaa gactttttta gcagaagatt cagttagtga tcgtcgctgc tccattgctc
     3781 gcagagtcaa acaagccatt cttgagctat taagcgaacc tctttctatg tctttgatcg
     3841 cacgtatgaa gcacatttct ccgaccactg tcattcgaat acttcgcagc cttcggccta
     3901 aaaccatttc tttgagccag ctgttaccag aagtggtgtg tttcgatgaa ttcaaatcag
     3961 tgaaaaatgt ctcgggagcc atgagctttg tcatgatgga cggcaagacc catcaactga
     4021 ttgatattgt ggaaaatcgg caactcaatc ctttacgcga ctatttcttg cgctacccta
     4081 taaaagtccg tgaacaagtg cgtctagtgg tttcagattt ctatacaccc tatcgcacat
     4141 tggtgaaaga gtgttttccg aatgcccgaa tcgtcgccga tcgctttcat attagccaac
     4201 acattggtcg ggcttttacc aaccaccgaa ttcaagtaat gaagaccttt aaaaaagggg
     4261 atcgtcggtc gaagcactta aagaaatact ggaaactact tcaaaagaat gcgtgggaat
     4321 tgaaaggtca gcatcgctac tggcgtccgt catttcgaga tcatctgaca gaagcagaga
     4381 tagtcgatcg gctattatct tatgatgatt ccttaaagcg aggctacgaa gtctaccagg
     4441 attttctttc cgttatcaga agacaagatg ttcctgaatt cgtcgcgctc ctaaaagaag
     4501 actacaagga gttgccagag cactaccaac cagtgttcac gaccttcaag aaataccaaa
     4561 cagaaatcaa acgagcgttg cgcgttccct attccaacgg ccctatagaa tgtttgaata
     4621 accatatcaa agtactaaaa cgaattgcct atggctatag aaactttcaa aactaccgag
     4681 aacgaatctt tttatatcga ggaaaatatt ttaagaaaac taagaatact acccaactga
     4741 ctaaagccag aacaacaaca cggctagaca agatagctgt ctagccgtgt gagtatctcg
     4801 attattctat tgaatttctt caccaacact cattgacgaa gagccaaaat aagtaatcta
     4861 accgactgtt aactaaacca ttgcaggtcc cgttaaagat acttgtgcct gttgtcaccg
     4921 cttctttttc ttgtccacaa gcgcttaaca cacttaataa agcccctgct attattaata
     4981 ataatatctt tttcttatta aaaacctcct catctatttc cagattagta aatgtagtaa
     5041 tctgtgcttt cttttttctc aatactaata actaacttat ggggtaaaca aatactcgtt
     5101 tgaccgggtt ttgagatcca tccagttcta accgcgattt gatcaggact attatcttct
     5161 ttaactcgaa ttcttccatc tttaatctca acaatattat attgtccctt cgcaggatga
     5221 taattcacga gtttatgatt gatcttatcc aaatcgaagc gatccatttc cttgccatca
     5281 attcgaacga tggcatattt gatagcatct tcagttgttt cattctcctt ttctatagag
     5341 aatacaaata agggaataaa agaggtaata atcaaaatgc caataattat gacatcgcct
     5401 atcttgacac cagcttttct taaaaatttc attaacgata tctggctaaa catattagac
     5461 tttttccatg gaaatggtga aatttttcgg aaattatctg cattaagaga atcagttaaa
     5521 caaccctcaa gttgttcctt aatacattgt tgatccatct tcataccaat aaaaactagt
     5581 ttattaatgc gttcaccaaa caacggatgc caggcttcct tcaattcggg atttttttgt
     5641 ataatcattt gacactcaat tttaggtaaa ctagctaccc aataatttaa tgattcaata
     5701 cttttatagc tacccgatgt ttcaaagatc gtcgaaaaat cactatgttg ggcaaaccaa
     5761 agaaaccctt ttgctcttgt aatattgcca ggaaaatttt tatccgtaag atgggcaaat
     5821 ttttgtgcgt caaggagtaa ccgatttcga tagataaaac ttgagatacc atattcttct
     5881 gtttcaggaa tatgattttg atagccaagc tctaactctt tgaaccagcc cactgaatag
     5941 gaagtctcat ctatattaaa aagatttgta tttagaattt tcgataagtc tacttcacct
     6001 ttaattgtcc taattatttt ggcattggac tgtaacttag caattagacc gttaattttg
     6061 taaatcgtct tggttgacac taagtcacta ttatttaaaa gtaatatgtt acaacactcg
     6121 aattgatcaa ttaacaagcc acgaatatcc tgttcttctt caactacatt accttcagct
     6181 tttctttcct gaatcttttc acccgtttcg aaattatccc aaaaacgttt tacatccact
     6241 actataataa tggcaccaat ctctaatcgt tgggtcaaat caatgtctaa agggttttta
     6301 gctaatataa ttgcttgaat aattggaatt ggtttgctga ttcctgaagc ttcaataaca
     6361 atttgattca agtcagaaaa ttgcactaat tgatctagtt caacaatcaa atcttcgcgt
     6421 aatgtacaac aaatgcatcc attcggcatc gaaacaagtt tttctccagt tcttttaaaa
     6481 cctttcttgt cgactaatac cccatcaaca tttacttccc ctatgtcatt tacaatgaca
     6541 gcaattttct ttccgtgatc accgtacaga atatgattta gaagtatcgt ttttcctgaa
     6601 ccaagatagc cagttaagat tgtaataggt atttttttca tagggaacct ctttctatac
     6661 cttgaattta cactttaagt gcaacacata aaaatgagtc ataatcgata tccaactagt
     6721 ataagtttgt cgagaacact cctagaagga aaataattat gagccagtat cgtcatctta
     6781 ctatactcga acgagaaaga atctttctgc ttcacgaaga aggattttct attagtataa
     6841 ttggcaacga cattcagcat agcccgttta ctattttaca tgaactgaga agaaacaaga
     6901 aaaaaactat tcaccttcca aagcggaaaa aaattatgct tatcgaagaa aaaaatgcgg
     6961 acgttatcac attctaaatg atccgtgagt ttggtcaatt gttcgtcatt tatttgtcga
     7021 ttttcagtgg tcgccagagg aaatctcgaa tcgtttgaag catgaacata tggacttttc
     7081 aattagtttt atcacgatct aacgtgcaat ttgcaatgat cttttcgata cagaacccct
     7141 ctcacacggc aataaaggcc tgatccgttc attgcaccat cgaggaaaaa caagacatac
     7201 aaaaaatcat gtcgagtctc gtggaaagat ttgccttact cattcgatcc atggccgtcc
     7261 aaaatccgca gatgatagaa gtgattttgg tcattggaaa gccgatattg tagctggcaa
     7321 aactggtggt acttgtttag tgactttgac agacagaaaa tcatgcttcc ttttagcagg
     7381 aaagaaggtc cccagaaaga aagccgctta tgtgagcgag aaaatgattg aacttttaag
     7441 cagtattcca aatgaggcgg tcaaaacgat cactccagat agaggaaaag aattctccaa
     7501 tcatttcgca gttacagaag cattgaataa tatgcccttt tattttccag atcctcatgc
     7561 ccttggcaac aatgaacaaa ttaaaatacg aatagactca ttcgagaata cttgccaaaa
     7621 tcgaaagaga tagctacggt agataactca acgataaatc agatgattga aaaactgaat
     7681 aacagaccta gaaagtgttt agattggaga acaccttatg aagtcttctt tgataaagtg
     7741 ttgcgcttga tttgacaatt caagacataa taaaaagcag atcaaatcca aataagggaa
     7801 aaatgatcta ctctggtatt atcaattaat tggccaagct attatgccgt tttgctaaac
     7861 gtctacttct tccagtattg accttgataa aacaacaatc aggatcccgt tttaatactc
     7921 tatgaactgt gtcaatacta acatgtaaag ttgcagcaat atcagctaat gtactcccat
     7981 ttaatctcat ttgctttgca aatcgataac gccgataagc atcaatattc ttggaatcca
     8041 tagacaataa tacttttctt accatgtatt tcctccaaaa attgaatttt aatgagccat
     8101 catttcttga gcaatttcat gtttagacaa tgaagctgga tagaaagtag gccaattatg
     8161 caattcatta tagagttttt cttgactttc tccaccaaag aaaatatgga aatgctctgc
     8221 cttacttggt gcaattccat gatcactaaa ttgaatatat ttaaaacgac cagcacctgc
     8281 atcatcagtt tcaaaattaa aacgaacacc acggtttcct ttttcataat tcaaaatttt
     8341 gtatccaaca tatttgtatg tgtattgatg atgttcaccg ttgactacaa aatcaatggt
     8401 gttgtcttta atgttgatag tatcaatatc tgtcttatag ccagtagtat aataatcttt
     8461 gtactcggca gctgtcatgt ctttattgat ttttgctttg taatcgaaaa cttcatccaa
     8521 aattccatct tttaacaacg gataaacaga ttgccattcg cctgtataat cactcaattc
     8581 gcgatccttc acatcggcat ctttaaagta cccattagca actgtctttt catccttcgc
     8641 ttctttttct ggaactacct cttcaccagc aaccatagtt gttttttcta acgcttttag
     8701 attgtcttcc attactgaaa cataatcttc accattatcc atttgttctt ttgtaagact
     8761 ttcaagagga ttcagtactt ccaattttat cccagcttcg ttggccaaag tttttgcaat
     8821 tttatcatta gcattctttt caaagtaaat ataattgatc ttattttttt taacatactc
     8881 ttttaattca gctaatcgac cagaagatgg ctcttcttca ggacttagtc cagcaatagg
     8941 aacttgtttc aatccataat ccaaagctaa atatccaaag gctgcgtgtt gcgtcacaaa
     9001 acttttttgt ttcgcttctt taagactagt ggtatagtca gcatccaacc gtttcagttt
     9061 agtcaaatac ttttcagcat ttgtctcaaa tacttttgct tttttcggat acaatttaac
     9121 taactgatcc ttgatatttg aaacttcctt aattgccatt ttgggtgaaa cccacgtatg
     9181 aggatctagt tcatgatggt gatcttcttc tccgtgatca tgatcttctt cttcactacc
     9241 tggcaataaa atcatgtctt ttgttccttc tactacatta ggcccagctt tcttccaact
     9301 ttctttagct tttggtaccc atgattccat gttttcgtta tgatatacaa aaacatcagc
     9361 atcatgagct tttgccatat ccttagctga gggctcatag tcatgaggtt ctgatcccgc
     9421 tggaaccatt aaatccacgt tcgcttcatc gccgacgatg tttttggtga aatcatacat
     9481 tggatagaat gttgttacaa tttctaattt tccatcttta tcattcgata cttttgcatc
     9541 attagtgctg gagcaagctc ccaataatcc tagagctgct atcccaataa gtcctacact
     9601 gattttcttc attctttttc cttccttaat tatgctaatt tcttcatttt atttcgttta
     9661 tgttctttga taatttttaa tgcccttttt ctagttttaa tattcgggct tttaagtcgt
     9721 cgatatgcac tagatatatc cgttttctcc attagaaatt acccccaatc actacttatc
     9781 attatttgct ttccgccagt aatttatcaa aactctgttt gatcctttct tcatccaaca
     9841 tttttccgat tagaataata gtggtttggt ctttttgtcg agtggtgtat tgaaaattaa
     9901 cctgttcgtt tacctcttgc atggcaacta taaaatcctg ctcttgtaaa gcaatcaatc
     9961 ctttaaaacg atacaggctc ccttggttgg ttaaaatcag ccagtcaatc cattggagta
    10021 aaaaatcttt ttttatgtct tgatctgaag ttaagagaat tgatttaaac tcatgtactt
    10081 gatggtgttg atgatagtat ttgccattat agtgattttc tgatgaatca ctttcaaaaa
    10141 ctaagggatg attaaatttt ttcaaattat aaaaatcctt
//
LOCUS       DAZAFF010000097         9945 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000097 DAZAFF010000000
VERSION     DAZAFF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.098"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACG9BB_002420"
     CDS             complement(<1..613)
                     /locus_tag="ACG9BB_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFN2838296.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            814..2028
                     /locus_tag="ACG9BB_002421"
                     /pseudo
     CDS             814..2028
                     /locus_tag="ACG9BB_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2207..2542)
                     /locus_tag="ACG9BB_002422"
     CDS             complement(2207..2542)
                     /locus_tag="ACG9BB_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFN2838297.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(2526..2789)
                     /locus_tag="ACG9BB_002423"
     CDS             complement(2526..2789)
                     /locus_tag="ACG9BB_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFN2838298.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(3249..4052)
                     /locus_tag="ACG9BB_002424"
     CDS             complement(3249..4052)
                     /locus_tag="ACG9BB_002424"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFN2838299.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(4030..5154)
                     /locus_tag="ACG9BB_002425"
     CDS             complement(4030..5154)
                     /locus_tag="ACG9BB_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2838300.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(5170..5985)
                     /locus_tag="ACG9BB_002426"
     CDS             complement(5170..5985)
                     /locus_tag="ACG9BB_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2838301.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5975..6847)
                     /locus_tag="ACG9BB_002427"
     CDS             complement(5975..6847)
                     /locus_tag="ACG9BB_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2838302.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6932..8221)
                     /locus_tag="ACG9BB_002428"
     CDS             complement(6932..8221)
                     /locus_tag="ACG9BB_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2838303.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(8365..8877)
                     /locus_tag="ACG9BB_002429"
     CDS             complement(8365..8877)
                     /locus_tag="ACG9BB_002429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838304.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8944..9087)
                     /locus_tag="ACG9BB_002430"
     CDS             complement(8944..9087)
                     /locus_tag="ACG9BB_002430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFN2838305.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            complement(9393..9551)
                     /locus_tag="ACG9BB_002431"
     CDS             complement(9393..9551)
                     /locus_tag="ACG9BB_002431"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838306.1"
                     /translation="MTVHWEGAHRPIARETQGGICEKMTEEILYQRLGSTSRNLIVSL
                     GHSMGISH"
     gene            complement(9570..>9945)
                     /locus_tag="ACG9BB_002432"
     CDS             complement(9570..>9945)
                     /locus_tag="ACG9BB_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN2838307.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         3054 a         2110 c         1610 g         3171 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccac caacacaaaa tattatagaa gcaaaatcaa ttctacctta gatgttctta
      781 tatcactcta tcagtcctat ttgacaaaga gccaagaaaa agacgttggt cgtcgatgct
      841 gttctctcgc ctactcctcg tgcttgtaga agttgtggtt ctactgtggt agatggaaac
      901 gggaaagcaa ttatagtgaa aaatgggaag aaggaaacga ttgtccgttt tgaacaatac
      961 aatcatatgc ctttggttac gcgcctaaaa aagcaacgct atacctgtaa aaactgtcgt
     1021 acccattgga cggctcaaag ttattttgtc cgactcagcc attcaatcgc agatcatgtt
     1081 cgatataaaa ttgcttcttt actgactgaa aaagtatctt tgtcttttat tgcgaaaagt
     1141 tgtcaggtat ctttgactac cgttactcgt acattgaaag agtttaaaag ctatttacca
     1201 aagccttcta agaagattct ccccagagta ttgatggttg atgaatttcg ttcgcacgct
     1261 tccatagaag ataaaatgag ctttatttgc gcagatggtg aaacaggaaa cttaattgct
     1321 gtcttgccta cgcgtaaatt acctcgatta acaagctatt tcttaggctg caccaatcca
     1381 gaagaagtag aattcttagt cacagacatg aacgccgctt acttccagct taccaaacgt
     1441 gttctgccaa atgcgaaagt agtgattgat cggtttcata ttgtcaaaca catgaatcag
     1501 gcactcaatg agttgcgtat ccgtgaaatg aatgagcttc gtaaagcagg acagaaaagt
     1561 caggcagaaa aactgaaaaa gaactggcgc tttctgctaa aaaatcgtgc aagcatcaac
     1621 cattatgagt acaaaacatg gaaaagtttc cgagcaccaa aatacccatt tctcactgaa
     1681 gcaatgatga ttgatcgatt gcttgagttt tctgcgcctc taaaggaggc gtatcccttt
     1741 tttcatgaat tagtcgaagc ctttcgagac aaagaccctg acttattttt ctccttattg
     1801 gcagaacttc ccgaaacgtt agatgacggc tttcgggaga agcttcaaaa ccttctgacc
     1861 tatgaagaag gtatcaccag cgcaatgatc tatccttatt ccaatggaaa aattgaagcg
     1921 aagaataccc acataaagac aatgaaacga gtatcctacg gatttaaatc attcgagaac
     1981 atgagaatta gaatcttttt gatcaatcaa ttaatcaatg taagataaca aaaaatctga
     2041 accagaatgt ggttcattct gacccggatt tgattttgca caactcatca gtccttattg
     2101 acaaagagcc aaaaatctga gccagaatga ggttcattct gacccagatt tgattttgta
     2161 caacccatca gtccttattg acaaagagcc cttcttttta gtattattat aattcttcca
     2221 tgcgttcttc cagtcgctgt aaatcgcttg gacttaattc cccaattttt ttccatttga
     2281 cctttttgtt gtttttgggt aattcgttta gagttcctac gtcaatccaa gattcttttt
     2341 ttagtcctgc ctgtttccaa tcccttattt caacgtattt cagctttatt ttatcggatt
     2401 tagtatgata ttttgaagta attgacagaa acgaaattgt gtccagtcca ttttttagaa
     2461 tatagacagg ccggcgttta ccgccctctg cagctgtgaa agcaacgtag acggttgcta
     2521 tatcattagt attcatcttc attgaaccat tcttcaattt tttcgtcagt gtctagtttt
     2581 tggacaggca atttttccgc taatctttga atattatgga ttcgtttact tttttccgac
     2641 aattcaatag aaaatgggat ttttccttgc tcaacaattt gcttataaaa aacggtgacg
     2701 gctgttttgg ggctcatgcc tagttcatca ataatgcttt ctgcttcatt tttcaatttt
     2761 tcgtccatag aaatttgtaa cttagccatt ttctccacct ctttctgaca aacacattat
     2821 atcacggaaa gagttcaaat ggaattaata caactacttt tgaactcctt gaaatgcagt
     2881 gtatttatct aaatataagg aaaggatata gtgctaaatg gccttgatag atagaaaagt
     2941 ttcaaaatcc gccacagcga taattgaagc gaacactcaa cgacacacag aacaggaaaa
     3001 gactcctaaa agaaccaaat taactaggac gtgtctgaaa actccacaaa gatgtggtat
     3061 gctctggtaa aaaacgaatg gcacaaccac gaagaccgat atggttcttg gaaaactttc
     3121 tccacccttt ttgtaattgg agggatagtg gtttattgat agcgattttc caagttcttc
     3181 aagtggaacc agacattgag caccacggct aaaaagtgaa tgaagtcttt atttttaatc
     3241 aataattttt aatgattacc agtaattggt tttttcatgc gccctatttc tatttgatgt
     3301 ggagttaaat aatgctgata agtaaaaatt tttccgtcaa ttaaactgcc actagtgaat
     3361 cctgttctat tggtaaaaag agaatagtct gggtattcat ctacaccaaa tgataaagat
     3421 tcagccataa actgtttttt accagcaaat tcagcactgc taccttgatg gatatgtcct
     3481 acaaaaatac ctaaaatatc actttcggca atcaattcag caaaaccttc cggaacttca
     3541 gttacaatag atggatcttc ccacgacaat ggatgatgaa ccaataaaat cgttcccttt
     3601 tcagcaggtg tacccaacca ctcttgtaat tgaatcatct gtcctatcga tatttttcct
     3661 tcgtgctggg tatcttgagc agaatccaag gaaataattc gtaacccatt ttgattcgta
     3721 caatggatat actctcccgt ttcattgctt tcaacactca ttccttcaaa gaaaacactg
     3781 cgacgatcat gattcccacg acaaaaatag taaggtgtgg ttgggaaata ttgatccaac
     3841 attttccgta agacttgata atcagaaatc cccccttcat gtactaaatc acctgttaat
     3901 agaataaaat cgtacactgt taacgagagt tcttgtaaca atttttccaa tgccaactgt
     3961 ggatttttaa ttgttggaaa tagttcagca gtaaacaagt tagtttgata gttttctcta
     4021 aaatggatat cagagagatg caaaaagcgc atggctattc cctctttctt caataaaata
     4081 atcaggatat cctaaatttt cttgcgtctg ataatcaaaa aaatgcattc tttcaggttc
     4141 tatgcggaga ttgactgcat caccaatctg ccaagtaggt ttttcattaa ggacaatcat
     4201 ttctatacca tcgactttaa cggtgtacgc ataactttga cctaatagct cacgatactt
     4261 cacttgaccg tgtaaatcgc ttgtttcttt tgaaaaggtt acgtgttctg gtcttatgcc
     4321 taaatacaat gagccactaa cctgaagaag ttgtttttgc caagctggat tcaatgttaa
     4381 aaattgactg gctaccttta atgttccttg atcataagta actggcataa tattcatgcc
     4441 tggcgaacca ataaaatttg cagtaaaagt atttttaggt ttcgtataaa catttttcgg
     4501 cgtatcaatc atctgaattc taccttcatt caataaaaca atccggtcag ctaacgtcat
     4561 cgcttcaatt tgatcatgag tgacgtagac aaaagtttgt ttataacgtt gatgaatttt
     4621 taccaattct tttcgtgcat ccaatcgtaa acgcacatcc aaatttgata acggttcatc
     4681 taataaaaaa taattactcc gtttaaccac cgcacgagcc aatgctacac gctgccgttg
     4741 ccctcctgac aaatctttag gaagtcgatc ttggtatggc gttaaaccta acatcttcaa
     4801 agcttcttcc aaacggtgct gaatgtcagc tgtcttaatt ttattcgccc ttaatgcaaa
     4861 aataatattc tcctttacag tcatgtgagg atataaagca taattttgaa aaaccattgc
     4921 gatctctcgt tctccacaag gtagcttatt tgctagttgt gtacctaaaa aaagctctcc
     4981 actcgtaatt ttttccaaac cagcaatcat tctcagcaaa gtagattttc ctgaaccaga
     5041 tggtcccagt aacacaattc gttctccagc ggccacagct aaatcaattt cttttaaaat
     5101 ggtatgttct ccatattttt ttccgatatt tcttaattca agcgtttctc ccatgatttg
     5161 tcctccaatt tatttaatac ctgcagaaac aaaagtcccg ataatatatt tttgaaaaat
     5221 tgcatacaag actaacggta caaacattgt aattaaacta atcgccattg ctacggtaaa
     5281 atttgtccca ccttcagaac taatataggt ctgcatcgcc aacggcaaag tataattatc
     5341 tactgtccgt aaaattaata ccggccaaac atactcattc caagaggtaa taaaaaacca
     5401 aataccggta gaagtaatcg catgtctagt aagaggaaaa acaattttac tcatgacggt
     5461 tgtttgtttg atgttatcca atgccgccac atctagaagg ctcactggaa tttcccgcat
     5521 tgtttgcgtc aataaaaaaa tgcccatcgc atcagcaaat tgtggtatta tcacacccca
     5581 agtagtatca attaaattaa gttttgcaat caacaaataa ttaggaatca tcgcaacagt
     5641 aaaaggaatg aacatcgtac caatgatgat aaagtaccag attttcttcc ctttgattcg
     5701 gcaataagta aaactatatg ccgcaaaaaa agctacgcct aacttaacaa ccgttataat
     5761 tgtagcaata aaaaaagtat ttctcgtaat tttgataatt ggaagatttt cgaacaaata
     5821 gcaaaaattt tccaaggtaa acggctgtgg tatcaaacta gtaatcgtct gataggcatc
     5881 tttcaatggc ttaaaagaat tggccataat aaacaataat ggatagataa taatgattat
     5941 gatgaacata aataagagat gtggcaacca gcgtttaatt ttcatagtaa accccctttt
     6001 ccacaaattt aaatattacc cctaagaaaa cagcaaatat taataaagtt aaaactgaaa
     6061 ttgctgaaga ttctccgact ttatacattt caaaagcctt ttgataagca taatagatca
     6121 cgtttgcact accattattc ggtccaccac gagtgatgat cttaatcgga gtaaacacat
     6181 actgcagacc ttgaacaatg gtcatcacca ataaatatag tcctgtcgaa ctactcatag
     6241 gcaacacaat tgtaaaaaaa atgcgccaga tgggtacacg atccaactta gctgcttcga
     6301 tcatctcttg atcaattccc acaattgctg caaaaatgac aatgaaatta taaccaaaaa
     6361 ctttccaagc agtaatacct gcaatcacag caattaccca accttgtaaa ttcatccaca
     6421 taggcagtct taaaccaaaa tttcccaaca agacagcaat tggtcccgat accggattta
     6481 atatccatgt aaaaaccatc cccgccacta ccaaagaaat cacagctgga ataaaaaata
     6541 atggtttata caaattctgc cctttttcta taactattcc taataaaaag gatgtaatat
     6601 atgggaacac acagctcagc aagacaaata gaacggtata ggacaaagta ttcttcaaca
     6661 ctttatgaaa gactggatca gtcagcacct ttacatagtt ggcaaagcca acaaattctt
     6721 tagtgggggc caccatattc cagtcaaaaa aactcaaata gatcgtataa atcaatggat
     6781 aaaaaacaaa cacgcccaac aaaataattg ctggcgccag atacaaataa cctacatagc
     6841 gcttcatttc cgacagcctc ctcatcctta tgtcaaaagc agacgacctt gtttttcctt
     6901 tcttacaggc gctgtcgtct gcttgctttg cttatttgag taactcatta atttcctttt
     6961 gagtagcttt catcgtttca ttgacggaat tacctgataa aattttatcc cgcatatcca
     7021 acaatttttg ttcagcttct aatccagcat ctccaggaaa tgaactccaa ctgaccatct
     7081 tatccatctg atcaattgct ggtatcatca tctgattttc agctaagaaa gcttttaatc
     7141 cattttgggc gtctgcaaca tctttacgtg gtggtaaata accagtacct tctgtccatt
     7201 tagctaaaga ttcaacacta taaagatatt ttaagaaatc ccaagttgct tcctttttct
     7261 tatccgtttg tgatgtaaca gctaacattg cgccaccagc aggtagttta acttttttcc
     7321 cttcccaact tggtgactca actgctttag cagtaaaagt actatttttt tgaatgttgt
     7381 ccctttgagc aatagtggta tagaccatcg cgacgttacc atcaataaag ctttgcacac
     7441 cttgttccca cgtagtgtgt aatgccgtct ggtcttttac catcctttga tataattcat
     7501 aagcagcgat accatcttta gaagcaaagg tagctttgcc atcttttatt acacttgccc
     7561 cattactttc taaaattgct tgttgtgccc aattatcagc aggttcttga atatacacgc
     7621 catactttcc agttttttct tttattatct tagaaaaagt ttgaacttct tcccaagttt
     7681 ttggaccatt ttcatccaag cccgctgctt ttaatatatc ttgatttaaa tataaaaccg
     7741 gattactaat ggagtaagga atacccactt gtttaccatc tttttcagac agtgccatga
     7801 tattgtctaa aaaatgatct ttaaaataat cagcattatc cggatccact tcttctacta
     7861 aagattgagg attagtatat ttgaaatttt ctgcaaaata atctttaaaa gcccatccca
     7921 cttgtactac gtccggtatt tctttggagg caacagaagc ttgtaaattt tgcatcaagc
     7981 ctttatacat atcaggatta tattttgcta caaccttcac tttgtcttga gattgattgt
     8041 atttatctac taattcattc actgtttttc cacctagggt atccgcatta acatgccaat
     8101 actctatcgt tacacgaccc tccgattttt ttgttgtctt ggtattccca ctaccacaag
     8161 ctgccaaaga gatgacactt aaaacagaca caacccctat aacaagattt ttctttaaca
     8221 taacattaac ttcctcctaa tttctttatg gccatttttc taatcaagaa atattatgaa
     8281 ctaccaatgt aaatttctag ttaattttct gtaaataatt cgaaatttgt gcatttcggg
     8341 gtacagggtc actggacata tccatcaagc taagaataaa gcccccatat ggtcatgtat
     8401 cccatctcca aatctcttaa ctcctctgtt ttgattataa aatagtttat tctgtgctcc
     8461 tttcttccat aaataggccc atgcacaacg tgaggttata ttataaacac cacagcgcgc
     8521 aaggtactca atcgtttcta gttcattgac tgtccataca gaaaatgtca atcctagctg
     8581 ctgccccact tcacgggcca acttagttaa ccatccttca tgaatattga cgatttgaac
     8641 gctttgatag gctaatttct gtaaggtatc gatcaaatct gcttcggtaa tatccccaag
     8701 ttttttccct tcgaactgat tttcaatatt ataaaatata tgctttcccc aaatcgcagc
     8761 ccaagataat tgtacatagc cactaagaac aatttgtggc cagatttgat aggtttgtgc
     8821 cagcgaaaaa acggccattt ccaatccctc ttcttttaaa tcacagttaa tggtcatggt
     8881 tgccttctca ttggccactg cttgaaacac ctgctcaagg gttaccgaat ctgggacaac
     8941 ttcttacctg tcatgggcta gtattagcgt accatctgtt tgctgacgca catctacttc
     9001 aaaacaaagc tggcgatcca aacaaaagcg aagaaatgcc cagctattct cctccgtatt
     9061 gtcactacca gagtgtgcag tgatcatggt cattttgacc cctcctttaa ttccgtgatt
     9121 ttctcccaca cgtccactat accgatgaag cgttaatttc ttgttaattt cttgtaaatt
     9181 ccctgcgttt tttcagtaaa ctagttgtaa atcttgcatg ctccttccta cgaactcatg
     9241 aatcgtcgat tttgcccttc tagctagttt caccaattaa gtagttctac attccccgct
     9301 tttaacctac tacacctttt tttgttttca gctattcctt cttcttgctg aaagtccttg
     9361 aggttctttc ttacaggata ccatcaaggt gttcaatggc ttattcccat tgaatgaccg
     9421 agacttacta tcagatttct gctcgttgaa ccaagtcttt gatacaggat ttcttccgtc
     9481 atcttctcac aaataccacc ttgcgtttct ctagcgattg gtcgatgtgc accctcccag
     9541 tggactgtca ctaccccttt ttgctatgcc tagcccacaa aaaaagctgt cctcacagac
     9601 agccagactg tagacaaaag ctgttccaaa ctcgtaactc gagcttggaa cagcttttta
     9661 tctacttcgt ttcgaatttt tgaacaaaaa aataaaggct ccttgccttt ccatagccag
     9721 gtggctaatt tctttaaatt cattgcagca aacactagca tcgtatgcgt cgcgactttt
     9781 tccaagcctc gatacttggt ccaacgcatc ccatgctttt cttttgcatc cgcaaatatt
     9841 cgttcaatcg tttgtttccg ttttttgtaa attgatttat tcagatctgt taggcgaaga
     9901 tgttccactt catccatcag atctccccaa acatgtctcg tgatg
//
LOCUS       DAZAFF010000098         9602 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000098 DAZAFF010000000
VERSION     DAZAFF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.099"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..2440
                     /gene="leuS"
                     /locus_tag="ACG9BB_002433"
     CDS             26..2440
                     /gene="leuS"
                     /locus_tag="ACG9BB_002433"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFN2838308.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            2788..4341
                     /locus_tag="ACG9BB_002434"
     CDS             2788..4341
                     /locus_tag="ACG9BB_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFN2838309.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(4722..6506)
                     /locus_tag="ACG9BB_002435"
     CDS             complement(4722..6506)
                     /locus_tag="ACG9BB_002435"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2838310.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(6521..8242)
                     /locus_tag="ACG9BB_002436"
     CDS             complement(6521..8242)
                     /locus_tag="ACG9BB_002436"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2838311.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            8342..9103
                     /locus_tag="ACG9BB_002437"
     CDS             8342..9103
                     /locus_tag="ACG9BB_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN2838312.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3112 a         1711 c         1925 g         2854 t
ORIGIN      
        1 tttcaataga acaggaggac agcaaatgag ttacaatcat aaagaaattg agaaaaaatg
       61 gcaaaaatac tgggcaaaaa cgaatacttt caacacccat gatgatcctg aaaagccaaa
      121 attttacgca ctggatatgt ttccctatcc atccggtcaa gggctacatg tagggcatcc
      181 tgaaggttat acggcaacgg atatcctttc acgtgtgaaa agaagtcaag gatacaacgt
      241 gctgcatcca atggggtggg atgcattcgg tctgcctgct gaacaatatg cgctagatac
      301 aggaaacgat ccagctgagt ttacccaaaa aaatatcgaa acattccgac gccaaatcaa
      361 ttctttagga tttagttatg actggaatcg cgaagtgaat acaactgatc cggagtatta
      421 taaatggaca caatggatct ttacgaagct ttatgaaaaa ggtctagctt atgaagcaga
      481 agtagcggta aactgggtac ctgaattagg aacagttatt tcaaatgaag aagtgatcga
      541 tgggaaaagt gaacgtggtg gctatgatgt agtcagaaaa ccaatgcgcc aatggatgct
      601 gaaaatcact gcttatgctg atcgcttatt ggatgatcta gaattagttg attggccaga
      661 aagcattaaa gaaatgcaaa gaaattggat cggtcgttca gtaggagcaa atattgaatt
      721 taaagtggct ggtacggata aatcctatac cgtatttacg acacgaccag atactctatt
      781 cggtgcaact tactcggttc ttgcacctga actagatctt gttagagaaa ttacgacccc
      841 tgaacaaaaa gctgctgtag aagcttatat cgaagagaca gctaaaaaat cagatctaaa
      901 gagaacggat cttgcaaaag aaaaaacagg agtatttact ggtgcttatg caatcaatcc
      961 agttaatggc aaggaaatcc caatctggat cgccgattat gtattatctt catacggcac
     1021 aggagccatc atggctgttc cagcacatga tgaacgtgac tttgaattcg ctcaaacttt
     1081 cggactagaa attcttccag ttatcgaagg tggagatgtt gaaaaagctg cttacacaga
     1141 agatggtacg cacatcaact ctgaatttct agacggcatg aacaaacaag aagcaatcga
     1201 taaaatgaat gaatggctag aagaaaatgg cgtagggaaa aaagaagtca gctatcgctt
     1261 acgtgactgg ctattctccc gtcaacgtta ctggggtgaa ccaatcccaa tcatccattg
     1321 ggaggacgga acagtcacag ctgtcccaga agaagagcta ccattacggt tgcctaagac
     1381 aaaaaacatc aagccaagcg gtactggaga atctccatta gcaaatatcg aggagtgggt
     1441 caatgtcgtt gatcctgtaa caggtaaaaa aggccgtcgc gaaacgaata caatgccaca
     1501 atgggcagga agctcatggt attacctgcg ttatatcgac cctcataaca aaaaagaact
     1561 agcagattat gaaaagttga agcgttggct accagttgac atctacattg gtggagcaga
     1621 acatgcggta cttcatttac tatatgcacg attctggcat aaattcttat acgatatcgg
     1681 tgttgttcct acaaaagaac cattccaaaa actatacaat caaggaatga tcttaggcga
     1741 aaacaacgaa aaaatgtcaa aatctcgtgg taatgtagtc aatcctgatg atgtggtcga
     1801 aacttacggt gcagatacac tgcgcatgta cgaaatgttc atggggccat tagatgcttc
     1861 gattgcttgg agcgaaaatg gcttagaagg aagccgtaaa ttcttggatc gtgtatggcg
     1921 tttgatcgtt gatgagaaca ataaaatgcg tgaccgtatc actacattga atgatggcaa
     1981 gcttgataaa gtgtatcatc aaacagtgaa aaaagtgaca gaagactatg aaaatcttca
     2041 tttcaataca gcaatctccc aattgatggt ctttatcaat gaagcctata aagtggacgc
     2101 tctgccttat gaatatatcg aaggatttgt tcaactactc gcaccgattg ctcctcacat
     2161 gggtgaagaa ctatgggcaa tccttggaaa tgatggtggt atttcttacg caccatggcc
     2221 aacatacgat gaagcagcac ttgttgaaga cgaagtagaa gttgttttcc aagtaaacgg
     2281 taaagttcgt gcgaaaagca atgttccccg agacttagga aaagatgagc tagaaaaagc
     2341 agcattagcc aatgaaatag tgcaagaata tattgaaggc aaaacagtca gaaaagttat
     2401 tgcggtacca aacaagttag tcaatatcgt tgctaattaa ttggctcttt gtcaataagg
     2461 actgatgagt tgtgcaaaat caaatctggg ccagaatgaa cttcattctg tctcagattt
     2521 tttgttattt caaattgatt aattgattga tcaaaaaggt tttgattttc atgttctcaa
     2581 acacgttcaa tgtctttttc ttctgaatgg tcttgtaaga gatctctgta atcatcaaat
     2641 ttttgtctat taatcttagt attttttgat gtataaacta cctgaaattg agagtacata
     2701 ccaaattcat aaatttttta ttagtgggac tagtgattgt atggattttg acgcataatg
     2761 aatgaagtat gagaaaagga gagacaaatg gatcaagaac tatttattat cgaagtatta
     2821 tcagctaaac aaaaaattat cgagaaagcg aaaaaaagag cgaaaagaat aatgttcaag
     2881 cttttaagct tagtattttt ctctggtttg ctttttttcg gagctgtatc gttcctatat
     2941 cgccatatga gtgcagtagt ccgttcagct gaagggatgc aaaaattaaa taatttgtat
     3001 cacttttcgt ggggagttgg cggaataaca attgttgtgc ttattgtttt tcttctactt
     3061 atttggctcc gttaccatca attgtcgcgt catttcgatc aatggcttag tcaaagagca
     3121 caacagtttt tacataataa accaacttct caagatgatg actatcttta ttttgagaag
     3181 acttcgacga gtcaagcttt tcggatacaa aaaagtgatt gccagttatt gctgagatca
     3241 cttgatgatt tacctatcta cttaggcgaa agcaaatcaa aattcggaat gaacacattc
     3301 catatttttt tactagaaaa cgaacccaga tgggaaaaaa gccagtcttc gcctggtgga
     3361 tacctgacaa ctaagatgag tacattagtc actggttttt tcctcattct tttagctgga
     3421 acaatctatt atgggagata cactcagcca aaaatgtctt ctcatataaa tgctgcttct
     3481 cacatgacaa tgaactcaac taacgatacg catatacaaa agacaaatga agggttgtta
     3541 gctgatccta agcaaaaggt aaatttgaca acacaaaaga taaatgatct caaacttgat
     3601 acagagacgc actctctcta tatgacaaca gatagtggtg ctaattgggt atttgtgccg
     3661 attgattcgc aatggttgcg ttttggcgaa tacaccctga caacggggct gattcctgaa
     3721 ggttattgga tggacaacac atatgatatt tccgccgatt tctcatggtt tatctattct
     3781 tctggggatg atgtacgtct gctgacttca agagataatg gtaaaacatg gcaaacgtct
     3841 attatcaccc aatcagttca gccgatacgt tatcgaaaag cggtatttta tggcaacgat
     3901 ggtctattta tctgtacatc agccagtggg atgtctgcag aaactttgta catttacact
     3961 actatggatg gcggaatcac ttggataaaa agtggaagta caatgatcaa tcagccagta
     4021 caaaatgtca gcttcgttac aacttcttta ggttttgttt cgacacgtga aagtttgttt
     4081 tatacaaaca acggaggacg ctcattcaaa gaatcagttc tgtcgattcc ggaaaactat
     4141 cagacagggg ggatagatct ttttcaatca ccaaatgaag ttactcaaat aagcgcgaat
     4201 acgttagaga caaaatttta tttgttgaaa aaaggaccaa tcgatgcagg gaaaatgttt
     4261 gcctgcctct tccgttcaac tgataacggg gaaacatggc aattcgttca gcaactatcg
     4321 caagtagaac aaggagatta aagaagagag attgtgagcc tgacgttcag caccgagtca
     4381 caagaaacaa attcaaaaaa cagcttctca tatttgttga atagtgtaac tcgtgattcg
     4441 gagttgacaa gagcaacaag aagcgatttt gaagtatagc ttctcgtatt ttacaagatc
     4501 gtgtcttatt gccgataggt tgtctcttga acaccgttta ttcgtaaaaa gggattgtga
     4561 caagtctttt gtcactactt accgaacgaa actgaaggag ctatccaaaa agtatcatta
     4621 gtattattct ttgtgtttct agtggcacca ctagtacatt taaaaaagct atcgatcatg
     4681 gactaatcat acatgatcga tagcttttta gataatgttt gttacgctat ctgaaattga
     4741 ctattataaa gactggcata aagcgttggt gattggagta gttcagaatg tgttcctttt
     4801 tcgacgatat cgccattctt cattacaagg atcatgtctg cattttcaat cgttgacaaa
     4861 cggtgtgcaa tcacaaaact tgtcctatct tttgtcacag cttccattgc ttgctgaatc
     4921 aatgcttctg ttcgtgtatc tacgctagaa gttgcttcat ctaaaataac gacaggagga
     4981 ttcgctagaa tgactcgagc aattgtcaat aattgttgct gtccttgaga taacgtgccg
     5041 ttttcgctag aaatgatcgt atcatatccg tctggcagtg ttcgaataaa gtgatcacat
     5101 tgtgcgattt cagccgcttc gataatttgt tctcttgtgg catccatttt cccgtaggcg
     5161 atattatcag ctaccgttcc ttcaaacagc caggtatttt gtaaaaccat cccaaaagaa
     5221 cgacgtagtt ctccacgtga aagcttggta atatcaagac cgtcgaattc gatctttcct
     5281 ttattcagtt cataaaaacg catcagcaga ttgactaaag tcgtttttcc tgcaccagtt
     5341 ggaccaacga tcgctaccgt ttgttttggc tttactgaaa aatcgacttg attcatcaat
     5401 attttatctg atgtgtaacc aaactgtaca tttttgaaag ctatttttcc tttcggttgt
     5461 tctaaaagtc ttgcatctgg aaggtctgga atttcatctg tttcatctaa tatctcaaat
     5521 atcctatcga ttgctgccat tgcggattgg atcgagttga tcacataaga agcggtagaa
     5581 attggttcag aaatctgatt gatgtactga agataagctt gtaagaagcc gatcgtcaca
     5641 ccacctgata gaacaagcat agcaccgaat accgcactta tgatgaaggc gagttgatta
     5701 atgaaacgaa tggccggata gatagcacag ttcataaatt gagcacgttt aaaagcgttg
     5761 tattgctttt tatttacttc gtgtatagca gcttctgcat gagcttgctg attgaatgtt
     5821 ttgaccacta gattaccagc taagatttct tccattcgat tatttaaaac acccagttca
     5881 tactgattct gttcagaaat ctgtttgttt ttgttcgcaa tcttagttgt aacaattgaa
     5941 ctgccaacga tcagcaataa gacgagcaaa gtaagcttgc tttcgatata aaacagcatc
     6001 agaccagcag cagctattgt tacgacagaa gtaaaaaatt gattgatacc tgtcaataac
     6061 acttgagaaa gttgattgag accggtagtt gtccgactaa tgatgtcacc tacttgatga
     6121 ttgtcaaaaa acgacatcgg taatgttttg aatttttttg tgatttcttt tcgcaccctc
     6181 aatgtgatct tttcgcttaa cgctgccatt gtccgttcct gaataaatga agtgatgctg
     6241 cttaagactg aacagccgat cagcaataag acagggaaca acaatgcttg tttgatatca
     6301 ctgaaagaaa gctgtccaat acctgttttt cttatttctg ataagagatc atcgatcccg
     6361 attcccatga tcaagggcat ggctacgact aataggttcc cgaataaact gcatagaaac
     6421 aatccgtaaa aatatttatg ttcaggtttg atcattttcc aaaaacgtat caaagcacgc
     6481 gtgttcaacg tttttttctt ttccatcgta tcccttcttt ctatattcct tgtgattttg
     6541 caaactcctg ataagtttgg caattagaca gtagttcttc atgagtacct tgtccaacga
     6601 tttttccttc atctaaaaca atgatgttat ctgcttgttg gattgtactg aggcgttgag
     6661 caacaatcac gagagttttc tccgacatgt gtttatgaag ggctgctctt aatgctgcat
     6721 ctgttttata gtctaaagcg gagaaactat catcgaatac atagatctct gctggtttga
     6781 tcaacgctcg agcaatacat atccgctgtt tctggccccc tgaataatta gaaccgcctt
     6841 gagcaacaaa gctgtccaat ccgtcaggaa gggtgtccac tagttcttct aattgagcga
     6901 tcttgatcgc tttagacaac tcttctgttg aggcatattc atttcccatc tgcaagttgc
     6961 ttaaaattgt cccgctgaat aagaaagcct tctgagggac ataactgatg tgggagcgga
     7021 tctgttcttg agtcatttga gtaagaggaa taccaccaag ggttactttt cctatcgtag
     7081 ggtcactaag tctcaagaga agtttcgcca aactactttt tccagatccg gtcccgccaa
     7141 cgatcgctgt tgtttttcct ttaggtattg tgaaatcaat atcttctaga actggatcag
     7201 agatttcagt atatttaaat gtgacatgtt caaaagcagc tgcatttgtc tgatcaaagg
     7261 ttggttcagc tactgttttg atcgtctctt cctcaatttc gatctctgct tgcagtattt
     7321 cttctatccg tcgtaatgag gcaaacgatt tagggataat gaccaatacc atagctgcga
     7381 tcatcaagta agcaagtgtc agcatagagt attcgactac agcagtcacg acaccgattt
     7441 gtaatgctcc ttctgctaca agatttccgc caaaccacaa aatagaagca taaacgattc
     7501 ccatgagcaa aaaagcaatt ggagtgaccc aagcaaatat tcggttcact ttgatcattt
     7561 gattagcata gtcagcaaag ctcttattcg ttcgcttttc ttcatatgat tgattatcaa
     7621 aggcacggat catattaatc ccagtaaaga attctctcaa tgtgatggtg atccgatcca
     7681 tttttggctg gatactcaaa gataaaggtg cccctcgttt catcagtcga taaacaatcc
     7741 atacataaaa aacaacagag atcaatgtaa cccatgttag taatagagaa taacgaaaca
     7801 tcatgagaat ggtgaaaacg caaataaccg gtgctggtaa gatcatttgg caaaataaaa
     7861 caatcatttg ctggacatta tcgacatcat tcgtcattct tgtcaaaaga gatccagaac
     7921 taaaacgact gatatccttc attgacagct gttggaattt acggaaatat ttctcccgcg
     7981 tttggaatcc aaacctagcg gaagccaatg ctgaaaagta actagaggca actgcagcga
     8041 ttgttcctag aaacgctaca agcagcattt caactccaat tttttttata actgaggctt
     8101 cgccacttgc tatacctgtg tcgatcagct ttgctactaa ttttggtaca cctaatgtac
     8161 cggcgatttc taaacagatc atcacaaagg taaggagagc gagtcctttg tttttctgta
     8221 agaatgtttt taagatagac atattattcc tccttttttc ctgatacttt tcgattctat
     8281 accttcaagt aacttgaagg tcaatagaaa aaagaataaa ctgaaaaaga ggtgagaaaa
     8341 aatgaaaaaa atgtatacga ttggccaagt atcggaacta tttgatttgc caaaatcaac
     8401 gatccgctat tgggatgaac aaggattaat ccgttcaagt agacaagaag agaatgatta
     8461 tcgattgttt gatatcgatg atatttttat gctttatgat atcgtttttt atagaaaatt
     8521 agatataccg atcaaacaga tgaagaattt atatggaaaa acattgacgg agttatatga
     8581 gacacttgat gaaacagaaa gaagaatcca taaagaactg gtggtgatga aacaaaaaca
     8641 aaaagagata agggagcaaa aaaaacagtt gaaattgatg atcgatacaa acgaggaaga
     8701 atttcctgta gaagagatcc cttttgactg tatgatctca acggagtttg aagatattgt
     8761 ggaaatcaaa aaatttctac ctaactattc aagctttggg atgatgagta tgccagcttc
     8821 cagttcacag acgatgtatg gattttttat cgaccctagt gaagtacatt tatttactca
     8881 agacgtgatt tgggagaaaa aagatacggc tgtttatcgt cgattcttat tgaaatccga
     8941 gatgaaccat gcggaacgta ataatatttt agaaatcaga gaaagaatgt atgaaaaagg
     9001 atggaaaaca ggagaagtga tcggccagta tctattgacg aatacggatg aaaacaatat
     9061 cagaactgaa tattatcatg catggataga aatgaaaaaa taggaaatat gtgtttaaga
     9121 taactcgaaa atgatggttg ttttagttat aaagaaaaat tttctggttc gattacgggt
     9181 ttaatgtggg ggattattag tcgtggatag atagaatttt agttttttag aggaaatatg
     9241 tctttaaaaa tggaatggtt gcaattctta gcgatatata cgatgtaatt gtcctcttaa
     9301 aaaagtatgg aaaaataatt gtattgggtg ttatgtataa ttcatggtga actgagacta
     9361 atgagaagaa aaattcatat tgttatgagt gagattgtat cagatgagaa tgataaatta
     9421 aataggtgca taaaagcatt acacttaaaa tacattgttg cttacattat tacaatatgt
     9481 taataaggca aaaataaata gatggattga ataaacatat tttttaatca aatagtccct
     9541 actgtgactt tcctcagcta tcggtgatac taccgatagc atttttttgc ttataaaata
     9601 at
//
LOCUS       DAZAFF010000099         9471 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000099 DAZAFF010000000
VERSION     DAZAFF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.101"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..1108
                     /locus_tag="ACG9BB_002438"
     CDS             134..1108
                     /locus_tag="ACG9BB_002438"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN2838313.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1090..1551
                     /locus_tag="ACG9BB_002439"
     CDS             1090..1551
                     /locus_tag="ACG9BB_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2838314.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1601..2065
                     /locus_tag="ACG9BB_002440"
     CDS             1601..2065
                     /locus_tag="ACG9BB_002440"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2838315.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2079..2828
                     /locus_tag="ACG9BB_002441"
     CDS             2079..2828
                     /locus_tag="ACG9BB_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFN2838316.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2828..3658
                     /locus_tag="ACG9BB_002442"
     CDS             2828..3658
                     /locus_tag="ACG9BB_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFN2838317.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3658..4305
                     /locus_tag="ACG9BB_002443"
     CDS             3658..4305
                     /locus_tag="ACG9BB_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFN2838318.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4403..5179)
                     /locus_tag="ACG9BB_002444"
     CDS             complement(4403..5179)
                     /locus_tag="ACG9BB_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2838319.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5323..6282
                     /locus_tag="ACG9BB_002445"
     CDS             5323..6282
                     /locus_tag="ACG9BB_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN2838320.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            6399..6707
                     /locus_tag="ACG9BB_002446"
     CDS             6399..6707
                     /locus_tag="ACG9BB_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFN2838321.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            6734..7033
                     /locus_tag="ACG9BB_002447"
     CDS             6734..7033
                     /locus_tag="ACG9BB_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFN2838322.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            7207..7758
                     /locus_tag="ACG9BB_002448"
     CDS             7207..7758
                     /locus_tag="ACG9BB_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFN2838323.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            7904..8197
                     /locus_tag="ACG9BB_002449"
     CDS             7904..8197
                     /locus_tag="ACG9BB_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFN2838324.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            8190..8486
                     /locus_tag="ACG9BB_002450"
     CDS             8190..8486
                     /locus_tag="ACG9BB_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838325.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            8557..>9471
                     /locus_tag="ACG9BB_002451"
     CDS             8557..>9471
                     /locus_tag="ACG9BB_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFN2838326.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTT"
BASE COUNT         3349 a         1359 c         1773 g         2990 t
ORIGIN      
        1 cattttatta tataaaattg ttataaaaaa atttttttga aaaatagtat tgcaaaataa
       61 agcaatttca tagataatag gtattgtaaa gttatatgac tttataatac ttattagagt
      121 gagggataaa gaaatgagac aactacacaa agaacagatt gaaacagcaa tggcagcgtt
      181 aaaggaaaag acaattgatc atgtatattt tacagcatgt gggggttcac aagccgtttt
      241 aatggctggt caatatctat ttgataaaga attaactgta ccttgtcatg tttatactgc
      301 taacgaattt aactatgcta taccaaaagg attatctgaa aattctttag ttatcacttg
      361 ttcccattct ggcacaacac cagaaactgt agaagcaaca aaaaaagcaa gagaatttgg
      421 agcaatgact atttgtttat ctaatgaaga aggatctcct ctatgggaag cagctgaata
      481 tcctgttcac tatgattggg gtaaagatgt tgatgctagt gatttaaata aaggaattct
      541 ttatggttta ttatttaata ttttacttgt tttagatggc tctaaaaaat gggaaacttg
      601 cttgaatgaa tttgataagt taaatgatct tgttattgct gaaaagagca aatttgaaat
      661 aactgctaaa gaatggggta aaaaccaaaa acgagataaa attatttaca caattggtag
      721 cggtatcaat tacggagaaa tttactccac agcagcttgc tggttcatgg aaatgcaatg
      781 gattaattct aatgctattc attctgggga atttttccat ggtccttttg aaatcactga
      841 ttatgatgta ccattcatct tagtgaaaag tattggagat acaagatttt tagatcaacg
      901 ggtagaagac ttcactaaaa aattcagtaa taaattaatg gttttagacc aagctgattt
      961 tgacttagaa tctatagata gtgtagcgaa agaatatatt gcagctgtac ttagtggcgt
     1021 tgttattcgc caaatggtag aagcgattgc ttttgaaaga ggccattcct tagatgttag
     1081 acgctatatg tggcaaatga aatattaatt aataggaggt tgcaaaagat ggcaaatcaa
     1141 ttatttcaca ttgtgattac aacacattct accctttgtg agggttattt aaaggcaaca
     1201 aaattaattt taatgaatga cgccacaggt attagtgctg ttccatttaa agaaaatact
     1261 agtccagatg attttgagga gagtatgaaa gaaataatta gtcagcatca tttgcaacct
     1321 ctaattattt taacggattt aattggagga acacctaata atgtttctat taaacagttg
     1381 aacaacaata gaattcaaat tattgctggg attaatttac ctcttttact ggagttatta
     1441 atggcccagt cagctggtaa atctatagaa gaaatagatt ttaaggaatt actgaataac
     1501 tctcaaaaaa gtatggttca tattaattcg cttttaaaag atgtcagtta aggtggcaat
     1561 ttctattcta taaattttaa aaattgaagg aggaaataga atgattaaat tagtgcgtgt
     1621 agaccacaga ttacttcatg gacaagtaat attttcttgg actaaacagt atgatattaa
     1681 ccatattatt gtggctgata acaaagttcc aggagatcca atgtctgtga tggctttgac
     1741 aattgctaaa cctgctgatt gcaaattaga cattgttaaa attagtgatg ttaaagggat
     1801 tatggaaaca tccccaaatg ataattacat gattttaatt aaaggaccta aggaagcttt
     1861 tgaattagtt caaaatttac cagaaattaa agaaattaat tttggcggtg tgtctaagaa
     1921 aagcgagtcc aaacaatatg gaaaggcagt ctttttaact cctgaagaat tagaagccac
     1981 aaaaaaaatt cttgatcaag aagtgaaaat atttgtgcaa caagtcccat cttcaagtat
     2041 tgaaaatgta gattttagta agtaaggggg gaaaataaat gttattaaaa gccattttat
     2101 taggtttagt tggtgtggta gctgttattg actccaggtt aattggtcga caaaatattg
     2161 gtagaccact aattttgagt acacttgccg gacttgtgct aggtgacttg catacaggga
     2221 ttgttcttgg ggcatcttta gaattaattt ctatgggatt cgtctcaatt ggggcggctg
     2281 gacctcctaa tatgcaactt ggtagtatta ttgccactgc ttttgcaatt ttaactaata
     2341 gtagtacaga aacagcctta acaatagcta tcccagtggc tgtagctggt gaatttatga
     2401 gtattatcat gcgcatgata attgctcaat ttggacatgc tgcagataaa gccattgaaa
     2461 ataataaatt tagaaaagct caaattattc atatttattg gtcatttatt tttaatgctt
     2521 ttgtttactt tattccaatc tttttaacag tatattttgg tgctgacgtt gtggcagatt
     2581 tagtagataa aattccagaa atgattacag acgccttgac tgtctctgga aatatgcttt
     2641 ccgcattagg gtttgcaatg ctattaagca caatgttgag taaaaaatta tatccttact
     2701 ttatttttgg attctttatt gttgcctatt caggtctttc actaattggc gtgacaattt
     2761 ttgcagctct gattgccttt gtaatggatc aagttaaata tggaaaaaga gaagaggcgc
     2821 atggttaatg gaaaatagta cagaaaaaaa acgtttaatc acaaaaaaag atcttaataa
     2881 gatgtgtctt cgttcatgga caatcatgtg ttcgtggaat tatgaaagac aaatgcatat
     2941 gggatttatg tatgggatgg cgccaatttt aaataaagtt tataaaaatg atgaagagaa
     3001 aaaaagagaa gcttatactc gtcatatggc attttttaac tgtacgcctc aaatgacttc
     3061 atttattatg ggactttgtg cttctatgga agaacaaaat gctcaagcag aagaaggaaa
     3121 ctttaatact gaatctatct ccatgattaa aaccagttta atgggtcctt ttgctggaat
     3181 tggtgattca tttttccaag gtactttaag aattattaca tttgggattg gattgtcttt
     3241 tgctaagcaa ggtagtattt taggaccgat tttagctgtt ggcctttttg ctataccttc
     3301 tattttagct gttttttacg gcggtaaatt aggatatgag tctggcaata aataccttac
     3361 taaattgtat caagaaggtc ttatggatcg agtaatgcaa tttgcttcta tcattggttt
     3421 ggctgtagta ggtggaatgg tggctagcat ggtgccaatt gtcacccctt taaaattcac
     3481 tacaggggga acagaacttg ttttacaaga cttacttgat tccattattc ctggattatt
     3541 accacttgta tgtactttgg gaatatataa attgattaag aaaaatgtta gtacaaatat
     3601 gctcttatta ggttgtattt tggcagggtt tgttttaagt ataataggta tcttataatg
     3661 attaaggctc taatttttga tatggacgga gttttaattg atagcgagat tatgtatcaa
     3721 aagtgggtat ttaatttttt aaaacaagaa aatttaagct atcctttgta ttcttatcaa
     3781 aaaaagattg gaactacagc aagtattttt gatgatatag aaaagtacaa tgagggatgt
     3841 aacagtttag aattgaaggg aaaatttact aaatattgga aatcgcaaaa gattgattac
     3901 ttggatattt ttcctaatat actaattgaa cagcttgaat ggttaaaaaa gaaaaaaata
     3961 aaaatggcaa ttgcttcttc cagtccaatg gatactataa atgaagttgt taccacttgt
     4021 aaaatagagt catattttga ttgtttaatt agcggaagag atttgccaga aagtaaacct
     4081 aatcctacta tattccttaa agcttcagaa cagctcagaa ttccagttga agaatgtctg
     4141 gtgatagaag attcttataa tggtataaaa gctggtaaaa gagcaaatat gaaggttctt
     4201 gcaattaaag ataaacgttt ttctcaagat gtaagtctag cagatgatgt tatttacgat
     4261 attaaatatc tgcgacaaga agtacaggtg aaatttggaa tataaataag ttttttgatg
     4321 tatttagaga atattagtaa aaataaaaat catgcccaaa tctaatcaaa attgattgct
     4381 ttgggtatga tttcttttaa tatcaatcta ttactttagg atctgaatca tttttatgca
     4441 ccgtatctaa agtgatattt tctgtgactt caataacata actaattcta tcacaacgaa
     4501 ctgtggattg agaataatga aatggttgat tatttttatc tttgccaacc ttttctaaaa
     4561 taaatagcgg atctcctact atacattgca ataagtcgga aagctcaggt gttgcagata
     4621 aaccgtttat ttccataata gagctcgtag ctttgacatc atagtcatct tctagaagtt
     4681 tatataatga agtatattgg ctaccttttt caaataaagt tggaaatttt tcttctgatg
     4741 cgaaaaaatt atcaattccc atcggggcac cgtcttccca cattaaacgt ttaatattgt
     4801 aaactctatc agttacattg atttgtaatt tattagctaa aaactcatca gcctttataa
     4861 ttttagaatg taaaacagta tatctaattt tatgtcctcg ggtagaaaga gaatcgctaa
     4921 atccatgaac tccagctaat tttaaacttt cttttttttg agttacaaag gtccctttcc
     4981 cccttaatcg ttcaactgtt ccactttctg ctaaaagttt aaaagctttt cgaatggtaa
     5041 tcttacttac tccataaatt ttacataatt cttcttctgt aggtaattgc tctcctggaa
     5101 gatatttttt ttcacgaata tcgttacgta tagaagcttc tacttgtaaa tataatggtc
     5161 tattatcttg aaaattcata gtacactccc taaaaagtta tacttcatta tatcacaatt
     5221 tattaaaacg tcgattgtaa aattaagcta gacaactaaa aagatcattt gtgctacact
     5281 caaaatagtt ccgaccaaag aattaataag gagtgagtac aaatgactta cacccatctt
     5341 acaacagacg aactagtaat catagagtct tatttcaata tcgagaaacc tgtcgctctt
     5401 gtcgcacaaa gtttaaatcg ctctagacaa accatttata atgtttattc gtttctcaaa
     5461 caaggaaaaa aagtgctaga ttattaccgt caatacaaac aaaataaatc tcgttgtgga
     5521 cgacgttcaa ccgttttatc tgatgaacaa tcaacatatg ttcaagaaaa ggtagctcaa
     5581 ggatggtcac ccgatgtgat tatcggccgt ggcgagtttc caattgattg ttctgttcgt
     5641 acactctatc ggatgtttaa aaaaggcctt tttgacacac agtcgttacc tatgaagggg
     5701 aaacgtaaac cgaataatca tcaagaaaag cgtggaaaac aggcctttcg cagaacgatt
     5761 catgaacgga acagtctcta tccacacctg actaaagaat ttggtcatct cgaagtcgac
     5821 accattgtgg gagttaaaca caagagcgct gttattacat tggttgagtg tttatccaag
     5881 gttattatta ctttgaaacc gacaggcaga cgagccgaag atatcgaaaa caggttaaat
     5941 gattggttca aatccatgcc taaacacttg tttaaatcca tgacatttga ttgtggaaaa
     6001 gaattttcca actggcgatc tataagtaac tcgaacgata ttgccatctt tttcgcagat
     6061 ccaggaacac cttcgcaaag agggctaaat gagcactcaa acggcttgct tagacgagat
     6121 ggcttaccta aaacaatgga ctttagggac acggatgaaa cctttattca atccgttgcc
     6181 tcgaaaagaa accatattcc aagaaaatca ttaaattatc gaacaccatt ggaagtattt
     6241 ttgagctgca tagataaaga catattgtct agcttaattt gacaaatcag atagtataaa
     6301 atgaatcaaa ttgaatatca aatatatatt atatccatga agcccaatct tctttaatag
     6361 aagattgggc tatttttatg gcaaaggagg gaaaagaaat gtttcaaaat aaaggacaac
     6421 gttatgtcac aaagggagta atggacagtt tgccggtgga attacaagct ttatgttgga
     6481 atctaatcga tcaaaacgtt caaaagcaat ttcatttaga ctatcttcaa atctttgaat
     6541 tttctacgga aaaaggaaat caaaagttgg ttcatcgtca agaagaacca gaagcaagaa
     6601 aagagtatct gatttcacct aagcttcgtt tgaaatcagt gtctcaaaaa atatgggtca
     6661 ttgattcggg tgagtatcaa acgatgttat taccagaaga atactgatat ctaaaagaaa
     6721 ggaacgaaag gacttgttag aaacggctat cgaagtactg gaaaagtgtg cacagctagt
     6781 cactactcct gaagagtggg gctatgaatc cgttacgatg gaaaaagagg aaattgaaat
     6841 gggtatgctt cctgaagatg ttactctacc acgtttagtg atgacacatc tttatattta
     6901 ttgcgcacca gaagatggaa aagactatgt agtttatttc attacagata ttaccagcca
     6961 gcgagaattt gttcgtgggt tactggtaga aggacgattg gtttggagtc agattggagg
     7021 aacgaatgaa taagatcgat tatttgagaa tgattgcgaa atgtttagaa gatatcctta
     7081 cagcgattca tcaaattctg aagaaaaaag gagaaagaat atgaaatatt ttcaactagt
     7141 attgacactt ataacggcaa tggtgattga attgcttgaa caaaaattag cataggagaa
     7201 aaagagatga ttgaaaaaga attagtgacc acacaaacag aagtttggcg agatcctcat
     7261 catacccatc gctatgtttt caaacgacaa tgggcgatca aaggaaaaga agcggaagat
     7321 aaattagcgg tagtcattac tattcgtcca acagatacag aaccatttac gaatgatttg
     7381 agtatgttat taatcgaaaa gaatatccga caattaggat ttactggttt tattgctgtg
     7441 aatctttttt cgtatacaaa agcgaaaagt cctcgggtat ttcctcgtgg aaatgatgaa
     7501 caatctttgg aaatattaac aactgttttc acagaaaaga aaatcaacac cattattttt
     7561 gcttgtggtt cgattacagc gacgagtcaa gtggcttatg aacaagcgaa gaccatttac
     7621 gatcaattga gtgcgaaaca aaaaaagatg accaaagtat tgataaatgc agaaggtaag
     7681 ttatcccacc cactaagtat tcatgttcgt aatgaatggc aattggcaga tagtcaatta
     7741 ttatttcaag aaaaataaca ttttattttc ttatgttact tataagtagt tttacatgct
     7801 ttttgacttt aaaaatacat gtgatgatac atgttatatg aattatcaat gtacccagta
     7861 atatgctatt ttcattatgt aaaaaataac agggggtgtg tagatggaga ttgatgaaac
     7921 ttgtttgagt aactcttata aaagtatgaa tgactttttt gaggaaatgg aactggattc
     7981 agaattgctc tatcaatttt atcttgcttt tcgagggcaa cagatttctt ttccaatgaa
     8041 aatgtacgat cgtgagttag ttagaaaaag aatcgaaaac atgatagaac aggaaaaaac
     8101 tgtagatatc cgtcaattga cagaagttta tggtttctct gcaagatgga ttcaaaaagt
     8161 aattaaacaa aaagaaagga gacgagtaca tggctaagct gattgtatta ggtggtttaa
     8221 tttacggtgg atattggtta tataaatata tgcttgcgca tccgaatata gataaaggaa
     8281 aagtaattaa gacagttggg gtaatcatag ttggactcat agctctaact aagaaaaata
     8341 atactaatag cccaaaaata atgagaactt atcgctgtag aggatgtgga agggaaattt
     8401 ctactagttt taatccatct gaaagccctc aaggaagaga tcgttgtcat gctggctatc
     8461 gacattcttg ggaaaggatt aactaaatga acaccattga gattaattga ctgataaaat
     8521 aagtacgcga gggagaaatt gaaaggggga aataaaatga aaaaatggtt gttattacta
     8581 attggaatgg ggattttatt cactggttgt acatctgaga atacgaggac taaagagact
     8641 gtagaaactg atacaacaat tgaggctaaa aaaagtaaaa cgcaatcaac tgaacaaact
     8701 acaacagaaa tgaaacaatc tgtggaagca agtacaagtg aaagttcaat cattgaatct
     8761 tcttctgaag aggttaaaaa tactttgtgg gatagtgaga aatctactca actagcaatt
     8821 tttgtcccac aatggggaaa gacattaggt caagaatata aaagctacaa tcctcagaac
     8881 aatgtaagtc tgtatggtac gcctttacca agtgcagtta ttgatggaaa ttggaaaatg
     8941 gcaatcaatg aagcaccagt taccgtacaa tggtcagaag atggaactgg tcatgcagat
     9001 ttcaatttag tagcagttta ttctgatgta gaaactggag aatatttagg gcagcatgta
     9061 tatttctttg gctttcaaaa tggacaacct aaagtttatt taacgcaaca aaatcaaggc
     9121 aatgataaaa actacatgta cttcaatgaa actcagaacc aacagttaaa acaaggtttc
     9181 attgatattg tgaatggaca aactcctcaa actccagtag tagaagaatc tactcaacaa
     9241 gcaacaacta caacgacaca atctacaagt agctcagcaa tttcaagttg ggaagctgct
     9301 aaagaaactg tactaaataa taaagcttta tggttgtatg attctcctga taaaaatgca
     9361 tcaaatacta atttaattag cacaaatgaa tatatgaaag atctaatgca agatgaaaat
     9421 ggaagctact attcaattaa tattgtaacg gatgataatg gaaatacaac a
//
LOCUS       DAZAFF010000100         9461 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000100 DAZAFF010000000
VERSION     DAZAFF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.102"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(192..860)
                     /locus_tag="ACG9BB_002452"
     CDS             complement(192..860)
                     /locus_tag="ACG9BB_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN2838327.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            994..1281
                     /locus_tag="ACG9BB_002453"
     CDS             994..1281
                     /locus_tag="ACG9BB_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838328.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1697..3235
                     /locus_tag="ACG9BB_002454"
     CDS             1697..3235
                     /locus_tag="ACG9BB_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFN2838329.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3232..4197
                     /locus_tag="ACG9BB_002455"
     CDS             3232..4197
                     /locus_tag="ACG9BB_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2838330.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4184..5506
                     /locus_tag="ACG9BB_002456"
     CDS             4184..5506
                     /locus_tag="ACG9BB_002456"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN2838331.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5569..5673
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5747..6490
                     /locus_tag="ACG9BB_002457"
     CDS             5747..6490
                     /locus_tag="ACG9BB_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2838332.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6505..7497
                     /locus_tag="ACG9BB_002458"
     CDS             6505..7497
                     /locus_tag="ACG9BB_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2838333.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7509..8246
                     /locus_tag="ACG9BB_002459"
     CDS             7509..8246
                     /locus_tag="ACG9BB_002459"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2838334.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8236..8958
                     /locus_tag="ACG9BB_002460"
     CDS             8236..8958
                     /locus_tag="ACG9BB_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFN2838335.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(9042..>9461)
                     /locus_tag="ACG9BB_002461"
     CDS             complement(9042..>9461)
                     /locus_tag="ACG9BB_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFN2838336.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3110 a         1619 c         1984 g         2748 t
ORIGIN      
        1 ttttttgagt ttaagcgaat tttttctccc aataagtaag aaaataatgg caaaataaaa
       61 aaacaaacta ttttcgcaga tcgtttgcaa aaatagttta tttttttata aagaagattt
      121 tactgaataa tatcatttta gtttacataa tgatatttct gtaaattaaa taaagtagtg
      181 aaaaataaga atcagtgctc taactgttca agaggatcaa gtggttcaaa tgtttctgta
      241 gtctgttttg cttccaattg gggcaaaaca gatccaattt ccagatactt cactttattc
      301 aatgtataag atagaataga acagctaagt aatgtataca gaaaatgtgt gagatccaaa
      361 taaagcaagg agacaactaa aataccaaaa gtaatcaaga aaagactttt gccaatctta
      421 atatgaaaat atttttcaat ggcttgataa atgatatctg ttccgcctgt gcttccgccg
      481 aatcgaaaga cgagaccgca accaatgcca gaaagaatac ctgtcatcaa actattgatc
      541 agtagactat ctacagcaaa atgtatatga aggctctccc ataaatgtaa aaagaaaact
      601 agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt ttccaaaaag aaacaatgag
      661 ataagcaata aaggtaaatt aaaaagcagt gaagtgacag aaggagcgaa tccaaaaaga
      721 ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg ccagcttaga tggaatattg
      781 attcctgtca cagctaatcc gtagattcct gcgcctaaaa tgattccgca caattctgta
      841 aataaattta attttttcat ttctagcctc ttttcttttg aaaattttca gcaacttctt
      901 tattatactg acttatactc ttacttcaag aggaaaatga aaaaaaatta tttttggctg
      961 tttcttttta tttatcaaat aaggagtgga aaaatgcgag cagcagactt attgacaatt
     1021 cttacaaaat cacccaaaaa aacagagtat caaggagttt ttttggctca agaacaaaaa
     1081 ctggtgcctg tgacaatggt ccagataaat aaagcaaatc aatttatctt tttatttgaa
     1141 cacggcaaag caccacttgc tatgaaagac atctttcttt ttttgatgaa gaaccgtgag
     1201 aaagctgttt attatcagtc aaaagaagaa ctcattcctc tttatggagt gaaagagata
     1261 gaaaataaag tggtgatata agtaaaaata agaaaacaag acgtttatga tgtcaaaaca
     1321 accatttttt acatttttgg caaaaaagtg tcatgataca gtataatatt cgttgaacag
     1381 gccaataaag gtgttctccc ccacgagatt tctactttat tggctgtttc tttttattaa
     1441 aataaaagtt gtgtgtgtaa aaaagatata gctgattcca agtgacaatt tcgtatagat
     1501 ttttatggta ttcgtagtga aaaaacgaga accaaaaagt tgatccgact ttgttgcttg
     1561 gattcttttt ttgtcagcat atttagaggt atttttttct tttttttgtt cgtgctgtaa
     1621 ggttaaggca agacaagtta gttgggaaaa aggaaagaaa tgtttatact taaagaataa
     1681 cagaaaggaa ggtcgcatga ataattttat acaaacatta agtgaaagaa aaggcgagtt
     1741 gctggaagct gcatttcaac atctgtccat ttctctcatt tctttgctga ttgcagcact
     1801 gattgctatt cctttagcga tttgggcagc caatcataaa aaaatggcag aagtcttatt
     1861 gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc ttattaggtt tacttatccc
     1921 atttgtaggg atcggaactg ttccagcatt gattgcactt gttatttacg ctttgcttcc
     1981 gattttccaa aacacgtata ttggtctagc tgaaattgac ccgtctattg aagaagcagc
     2041 ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa gtagagcttc ccatcgcatt
     2101 acctgtcatt atctcaggta tccgaactgc cttagtttta atcatcggta cggctacttt
     2161 ggcagcattg atcggagcag gtggattagg gacatttatt ttgttaggta ttgaccgaaa
     2221 tacacctgtt ttgacattga ttggtgcgat cagttctgcc ttgcttgcaa ttatattcag
     2281 cggactgatt cgctggttgc agcataagaa acctcgatat tccatcatta ctttagcagc
     2341 agtggtgtgt ttatttggcg gcgtttcttt atatcagtct gccttgttta aaaatgaaac
     2401 catcacgatt gctggaaaat taggagcgga acctgaaatc ctgatccaaa tgtataaaga
     2461 attgattgaa gacaacacaa aaacagaagt tgaattaaaa ccgaattttg gcaagacgag
     2521 ttttttgttc aatgcactgg atcaaggtca aatcgatatc tatcctgagt tcacaggtac
     2581 tgtcctagaa agcttggtga aggtacctga agcaatgaaa agtgctgatt tgacaaaaga
     2641 aaaaacatat gacgaagcag aagaattatt gaataagcaa ttcgatatga cattattaaa
     2701 accaatggct taccaaaata cgtatgcctt ggcagtcaag agatcttttg cgaacgaaca
     2761 tcaacttaaa aagatttccg atttggcagc tatctctgat cagatcaaag cgggattcac
     2821 actggaattt attgatcgtt cagatggata taaaggaatc caagaagcat atggcttgaa
     2881 tttttcaagt gtacaaagca tggagccaag tttgcgttac caagcaatca ataatggaga
     2941 tgtcaatatt gtggatgcct attccacaga tagccagctc aaggagtacg atctagtgac
     3001 tctagaagat gatcgagggt tattcccagc ttatcaaggc gcaccgctga tgaaaaaaga
     3061 ttttgctaaa gaacatcctg aaatcgttaa agcactgaat aaattaagcg gaaaaataac
     3121 agaggatcaa atgattgata tgaattacca agtcaatgtc gaaaagaaaa agccgacaga
     3181 agtagcacga atcttcctga aaaatgaagg attagtaaag gaggaagatc agtgactaaa
     3241 ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa agtatgtcat cgatgaccta
     3301 aatctggcaa tcaaaaaagg agaaatcttt gttttggttg gtccttccgg tagtggaaaa
     3361 acaactactt tgaaaatgat caatggatta tctaaaccat cagcaggaga catctacttc
     3421 aaaggaaaat cgttaaatga atataatctg cagaaaatgc ggtggaatat gggatatgtg
     3481 ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc aaaatattga agtcatccct
     3541 gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg tagatgaact tcttcaaaaa
     3601 gttggcttat ctcctgatat ttatcgagat cgtatgccta gagagttgtc tggaggagaa
     3661 cagcaacgga tcgggatcat ccgagctatt gctgcaagcc cagatgtgat tttgatggac
     3721 gagccattta gcgcattaga tccgatttca agaaactcat tacaagaatt ggttttgtcc
     3781 ttacacgaag aattgggcac gaccatcgtt ttcgttacgc acaatatgga agaagcaatc
     3841 aagctaggtg atcgtattgc atttatgaag gatggagaaa ttatccaatg tgacacacca
     3901 gagcagttat tgatgaatcc taaaaacgac tacgttcggc atttctttga tgaaccgaaa
     3961 cagacgaaag agtggcgagt agaagattta gtcgtcaatg gatatttctt aaatgaaata
     4021 ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta tgaaagaagt atattcgctt
     4081 ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa gaagtattgg gtcgctgaca
     4141 agtaaagaaa tctttgcttt tttaagtcga gaggaggaag gaaatgaaaa catatgatgc
     4201 aatcatcatc ggttcaggtg tgagcggctt aagtgctgct tatggattga aagaagcagg
     4261 taaaacagta ctagtggtcg aagaagatct atggggcggg acttgtccta accgtggatg
     4321 tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt aatcgggtaa aacaattgag
     4381 tggaaaaggt tttaacgaga tcccaactgc caactgggaa gaacttcaaa aattcaaacg
     4441 aacatttacg gatccagtcc cagaaagccg aaaaaaacaa ttagcagaag ctgaaatcga
     4501 tcatctttca ggaaccgctc gttttttgga tgattcatct attgaagtca acgaagaagt
     4561 cttccatgcg gattacttag ttttggcaac cgggcaaaga cctacgatct taccagtgga
     4621 gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca ttgcctgtac tgcctaaaga
     4681 aatcattttt atcggtggtg gttatattgc ttttgagcta gctacgattg ccaatgctgc
     4741 agggagcaaa gtaaccattg tccatcataa tcaacggcca ttaaaagaat ttgaggcgtc
     4801 tttggtagag gaagccgtcc atcaaatgga agcatctggc attcagtttg cttttggtgt
     4861 tgaaacccaa aagatcattt cagaaggtac tcgctatcgt ttagtcggaa aagagacgga
     4921 attagtagca gatatgattt tttgtgcgac tgggagacag ccgaatacag aatctttagc
     4981 acttgagcaa gcgaatatcg tctttgataa acacggaatt gcagtcaatg attatctgca
     5041 aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt tcaaggaaaa ccccaaaatt
     5101 gacaccagtc gccacttttg aaggaaatta tgtagctaag cggataacgg atgcaacaag
     5161 cgaacctatc aaatatccga ttatacctac gatcgtctac gccagtccta aacttgctga
     5221 agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa gtggtagaga tggatcttac
     5281 cagttggttc acctatcatc gtgtcaatga accagttgct aaggctgaat tgacctttga
     5341 ccagcaaaat tatttgattg gtgctgcagt gattagcgag caagcagacg aattgatcga
     5401 tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa gagttggata gctatatcat
     5461 ggggtatccg acattggcaa gtgatctctc ctatttattg aaataatcag catgataact
     5521 aaagcgcttg ttgctttttc cttcttgtgc tacactttaa aaaatgaata cctgtttctg
     5581 gggatgtcgt agacgacatg agagtaagtt gtgaacttag acccattgaa cctgtcagtt
     5641 aatactggtg aagggagaaa catagcagac gcttgaagcg ggttactatg tcgtgcccat
     5701 ttcaagcgtt ttttgttatt catattagaa gaaaggaggt aacaaaatga agaaaaatct
     5761 ttatccagca gtcagtatgg cattcatttt aattatttgg cagattgttg tcatagtttg
     5821 gaaaatacct gcctttattt tacccggacc tgctgctgta ttttctgcat tagcggcaga
     5881 tgcacaagta ttactttccc attctgtcac gacattaaat gaagcagtgc ttggtttggt
     5941 aatagctagc gttctttctt ttattactgc cttggcaatg gattattgga agtttttaga
     6001 aatgtctttt tatccgttac tagtgatttc acaaatgttg ccaatcatgg tgttagggcc
     6061 attacttacc ctatggtttg gtttcggtat gcttccaaaa gtgatccttg ttgtcttgat
     6121 aagctatttt ccgatcgtag tttctttttc ggatacattg aggaaagtat caaaagagca
     6181 aatcatcttt ttgaaaacga tgggggcaga tacagggaag atttatcgaa tatataaaat
     6241 accagcgggt ttatccggct tcttttccgg tttaaaagtg gctgctactt attgtgtcag
     6301 cggagcagtc gttggagaat ggctaagcgc tcaagcaggc ttagggtatt acatgatacg
     6361 cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg atcatttgtg tcattctatt
     6421 gagtttattg ctgaatggcg cttgttcatt attgaaaaaa gggtattatc gtatgcttta
     6481 tattttttaa gagaagggga tcaaatgaaa aaattatggc ttttattacc gctactgttg
     6541 cttttatccg cttgtggcac tgcaaaagaa acaagtagca aacaagaaat aaaggatttg
     6601 aaaaaagtga cacttgtttt ggattatgta ccgaatacta atcatactgg catctaccta
     6661 gcaaaagaaa aaggctatta taaagaggct ggtttaaatg tccaaatcat cgaaccagga
     6721 gataattcga caagtattgg tctagtaggt gcggacaaag cacaatttgg tgtaagttat
     6781 caagaagatg tcacttatgc tcacgcagat ggacagaata taccagtcaa agcaattgct
     6841 acagtgatca aacataatac ttctggattc gccaccctat cagacagtaa catccattca
     6901 ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa gtccaagtga agaagctgtc
     6961 ttaaaagctg tgatggaaaa agacggcggg gacttttcaa aactgacaat ggtaggaagc
     7021 aatggagaag ggccagaaag cttaggaaaa tcctcagata tccaatggta ctttgaaggt
     7081 tgggatatga tcaaagcaaa agaagcgggt attgaactaa actatatccc attgaaagaa
     7141 ttagatgaac ggttagatta ctatactccg gtaattatta cgaatgatca actgatcaaa
     7201 agtgatccag aattggtaca gtcttttatg gatgccacga aaaaaggcta tcaggaagct
     7261 attaaggatc caaatgacag tgctaaactg ttgcaaaaat atgcaaaaga aaatgatcgg
     7321 acgtttttag aagaatcaca agcattcttg tcaaaaaatt atacggatga tcctgagaat
     7381 tggggattga tggaagaaaa agtttggcgc aattatacag cttttatgca ggaaaacgga
     7441 ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta atcagtttat aaaataggag
     7501 tgatagatat gtcaatccaa atcagccact tgaatgtagt cattgaaaac ctgaaaatat
     7561 tagaagacat ccatttcact atccctaaag atacctttac atcgattgtc gcgccaagcg
     7621 gagccggtaa gtcaacattg ctgaaaactt taacaggagt ccagcctatg acttctggaa
     7681 cgatcaaagt agacgatcaa aaagtaactg gtctccatac agcatttagt tatatgccgc
     7741 aagaagatat gttacttcct tggctgagcg tctatcaaaa cgttacgtta tatcaaaaaa
     7801 tcaatcatct aacaatcaat gaagaacaag taagggatta tctggatatc tttgggttaa
     7861 acggctatga acattttttg ccggaacaat tatctggcgg gatgaagcag cgaacggcct
     7921 tgttacgaac aatcatgaat ccttcttctt acttgcttct tgatgaacca tttggagcgt
     7981 tagatgctat gaccagaggg cagatgcaag actggatgtt gaagctgcct aaaaaagcaa
     8041 agcggactac tttacttgtg acgcatgata tcgaagaagc aatttattta tcagacagga
     8101 ttcttgtgct ttccgcccgt ccggcacatg tgattgctga gattcaagtg cctgaaaaac
     8161 gacggtcacg tgaatggcta ctgcagcaat cagagttgaa gcaaacgatt tatcagttat
     8221 tggcaggtga attagatgtt aagtgatgct cattgtcatt tagatgggag tcagagttta
     8281 gcactccttc agcaggaaca cacgattttg acgatcatca attgtgattc gccagaagaa
     8341 tggaaggaaa acagacagct tgcagcaagt aagactcaag ccttaagtta cggtatccat
     8401 ccttggaaag cagatagcta tacgtttgaa caagttgagc cttttttgaa aaaagcaagg
     8461 atcatcggtg aaatagggtt agataatatt tggacgaatg taccgatgac tacacaaaaa
     8521 aaagtatttg agcgacagct tgcttttgca gccataaatg agaaacctgt tgtactgcac
     8581 acaaaaggat gcgaaaaaga gatcttaaat tatatccaag cttaccctaa tcactatttg
     8641 atccattggt attcttcttt ggactatcaa caagactata ttgatttagg ctgttatttc
     8701 agcattggtc ttgatttgaa aaagaatcca gcagtctggc aactagctaa agcagtccca
     8761 atcgaccact tacttattga aacggatgga ctacatgcag cagagtgggc tttagggggg
     8821 attttttcag aaaaagatta tgtctcactg cttgaagaac agttaaagac aatcgctgag
     8881 atcaaagagc tatcagttga agaagttaaa aaaaatactt atcaaaactt ggaacgtttc
     8941 attagattag gtaaataaaa aagagatgtg acacaactag tgtcgcacct ctttttggct
     9001 ttctttgtat aaaagtaaac tgtttgatta ataaatgaat cttagtacca accgtttgct
     9061 tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg aagtaacata ttgttctgct
     9121 actctttctt ggtttgcagc agagtagtca ccatttaagt aagaagaatc taattggtaa
     9181 cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc ttgattcttt ttgtgcgatc
     9241 cattcttttg cagagcttgt gcttgctgga gtagcagcag tttctgttac agcaggagca
     9301 ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt gaaccgtttc ttgtacaaca
     9361 ggttgttcag cttggactgg tgcttcttga acggctgctt gaggttcagc agctgcttgt
     9421 gcagagcctt ctgttgggat tgttaattgt tgtcctgagt a
//
LOCUS       DAZAFF010000101         9416 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000101 DAZAFF010000000
VERSION     DAZAFF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.103"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..388
                     /locus_tag="ACG9BB_002462"
     CDS             32..388
                     /locus_tag="ACG9BB_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HFN2838337.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="ACG9BB_002463"
     CDS             404..754
                     /locus_tag="ACG9BB_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HFN2838338.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="ACG9BB_002464"
     CDS             783..1562
                     /locus_tag="ACG9BB_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HFN2838339.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="ACG9BB_002465"
     CDS             1573..2235
                     /locus_tag="ACG9BB_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HFN2838340.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="ACG9BB_002466"
     CDS             2254..3360
                     /locus_tag="ACG9BB_002466"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFN2838341.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4441
                     /locus_tag="ACG9BB_002467"
     CDS             3350..4441
                     /locus_tag="ACG9BB_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFN2838342.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4454..5194
                     /locus_tag="ACG9BB_002468"
     CDS             4454..5194
                     /locus_tag="ACG9BB_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFN2838343.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5482..7422
                     /locus_tag="ACG9BB_002469"
     CDS             5482..7422
                     /locus_tag="ACG9BB_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFN2838344.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7509..>9416
                     /locus_tag="ACG9BB_002470"
     CDS             7509..>9416
                     /locus_tag="ACG9BB_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN2838345.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLL"
BASE COUNT         3094 a         1568 c         2114 g         2640 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacagggc gacatgtacc atgtctatca tccctataca gaaaaaatcg ctagacggga
     3661 aatcatcata cctaagggac ataatgtaga ttatgggaca ccggttgaag taatggttgc
     3721 tcaaggtgga ggaaaagtca tagaagctgg ctatgcaaat atgtgtacac cggaacatgt
     3781 ttccatgatg attacggaaa aaactgctgc gcttctatat attaaaagtc atcatgcagt
     3841 tcagaaaagt atgctgacgg tggcagaaat ggtagaagtt gccaaagctc atcgtttgcc
     3901 attgatcgtt gatgcagctg cggaagaaga tttattcaaa tatagcgaaa tgggcgttga
     3961 cttagtgatt tactctgggg caaaagcaat cgaaggtccg agtgccggac tggtcatagg
     4021 aaaaaagaag tatattcaat ggattcggct acagtctaaa ggtattggcc gagcaatgaa
     4081 aatcggtaaa gataatattt taggctttac ccaggcacta gaagattatt taaaaaatgg
     4141 gagcgaatcc ggtgactcca tgaagcaacg attagcgcct tttgttaacg caatcaatca
     4201 acttcctgac ctttcagcga aaatagttca agatggtgcc ggcagagata tttatcgagc
     4261 aagtgtaacg attaatggcg ataaaagtgc gaaagaagtc attcaggaat taaaagcacg
     4321 aaatccagca gtctacaccc gagaatatca agcaaataac ggaatcattg agttcgacat
     4381 tcgttccgtc agccaaaacg aaatggaaaa aattataaaa agattgagag aaatcatgta
     4441 ggaggctttt aaattgtcag ttatcccaaa ttattataaa aatcgtgtat gtttaaatgt
     4501 attagctcat tcagttgaaa atgcaaaagc atgctatgaa gcagcggaag ggcatgtcgt
     4561 actaggtgtg ctatcgaaaa attatgctag cgatgaagca gcaatcgaag atatgaaaag
     4621 gtaccaagac gcaaccaata atgcgttgtc agttggatta ggcgctggag atccaaacca
     4681 aagtcagatg gtttcgcgtt tatctggggt tttacaacca caacatgtca atcaagtatt
     4741 tactggcgtg gggacttctc gtgctttatt gggccaaaat gatacagtca tcaatggatt
     4801 ggtttcacca acaggtaaga tcggctatgt caatattgct acaggtccgt taagttcaca
     4861 agcgccagca gctgaagtgc ctattgaaac tgccattcaa atgctgcaag acatgggagg
     4921 tacttcaatc aagtatttcc cgatgaaagg cttagcacat agggaagaat ttaaagcagt
     4981 tgcggaagct tgcgcaaaat atgaattcta tttagagcca acaggaggaa ttgatctttc
     5041 aaactttgaa gaaattctga aaattgcaat cgatgcaggt gtaaaaaaag tgattcctca
     5101 tgtttatagt tcaattatag ataaagaaac aggagatacg cgtataggag acgtaaagat
     5161 actattaagc atggtaaaaa aaattattca ctaactcatt agaaaataag aagcttattc
     5221 cagaatgaaa ttaaatggaa taagcttttt ataattaatt acggaaattg ttaatgtaga
     5281 taataattta gatgtacgta agcagaagta cagaaatgta agattttcca atattatttt
     5341 attcaacata ataaatcgta tatcaatttt tttagcgaaa tttctattta ttccaagaat
     5401 ttcagttatt acggaatcat tttgtgctta ataaaaagaa accgttttta tatactataa
     5461 atgtagaaag gcggtaggaa aatgaaaaaa attattgcaa tcacatcctg tccagttggt
     5521 attgcccata catacatggc agcagaaaac ttagagaaag cggggaaagc agttggcgcc
     5581 gaggtaaaag tcgaaacaca tggttccatc ggtattgaaa atgaattgac tgcaagagat
     5641 atcgaagaag cagctggtgt aatcatcgca gcagatacta aaatagataa atcacgattc
     5701 ggcggtaaac ctttgataac cgtaggtgtg caagaaggaa tccaccatgc agatgagttg
     5761 gtgagagata ttttagcagg aaaagcgcca gtctataaaa gtacggaggc aattatttct
     5821 tccgaaaata aaagtgaatc agaaaattta ggtaaaaaaa tctataagag tttgatgaat
     5881 ggcgtttcgt acatggtacc tttcgtagtt acaggagggt tattgatcgc gatttcttta
     5941 acattaggag gaacagctac acctgaaggg atcaagatac cagaaggaac gatttgggca
     6001 acgatgaata gtatcggaag tatcgctatg gggttgatgg ttccaattct atctgcattt
     6061 attgcgcaaa gtattgcaga tcgtccagga cttgttcctg gatttgttgg tgggatgtta
     6121 gcggcaaacg gagcattata tggtagtgat gcgaatgcgg gattcttagg cgggatcatc
     6181 actggatttt tagcaggatt cattgcttta ggaatcaaga aagtgcgagt accgaaagct
     6241 ttacaatcga tcatgccaat tattgttatc ccaattttag gatcattggc agttggtttt
     6301 gtgtttatct atgttattgg agaaccagta gctcttctat ttagttcatt gactcacttt
     6361 ttagcaggga tgcaaggagg aagtgaaatt gttctagcta tgatcttagg agcgatgatt
     6421 gcttttgata tgggggggcc gttcaataaa gtagcctttt tgttcggatc tggcttgatt
     6481 gcagaaggca attattccat tatggggcca attgctgtag ctatctgtat ccctccatta
     6541 gcattggggc tggcatcatt gattttaaaa accaaattta cgaaaacaga acgagaagct
     6601 ggtaaggcgt ccttagcaat gggactgttt gggattacag aaggcgctat tccgtttgct
     6661 tcaacagatc cactacgggt gattccaagc attgtaatcg gggcaatgac aggaagtgtg
     6721 atcgcgatgc tttcaggagt gacagatcat gtagctcatg gcggtccgat cgtagcggtt
     6781 ttaggagcag tggatcacgt agccatgttt tttgtagcag tgattgctgg tattgctgta
     6841 accgtgctaa tgttaagaat ccttaaaccg acattacaac aaactgcggt tgctgcagca
     6901 aatggtgtga cagtagcaga tctatcagag actgaaacaa gtgatgttta tacgaaaact
     6961 gaaacttttg attctatcac ggatattatg tcagatgaag caatcgtttt aggtgaaaca
     7021 gcaacaacaa aagacggagt aatcgaagat ttagtccaac gtttagaaaa aacagcggcg
     7081 atcacagata aaaccagatt taaagaagtc atctatgata gagaacatga aagtacaacg
     7141 ggaatcggta tggggattgc tattccacat ggaaaatcag cttatgttct taaaccaaca
     7201 gtggcatttg ccagaagtca atctggggta gattggcatt ctttagatgg aaaacctgca
     7261 catatgatct ttatgattgc tgtacctgaa aacagccaag gtgatatgca cttgaaaata
     7321 ctgcaacgtc tatcacgtaa attgatggat gatgacttcc gtcaagcact gatggaagcg
     7381 ccagataaaa caacagttca ccaattatta agcgaaatgt aacataagat gaaaaatcga
     7441 ccgtgatgag acataataga tgtgttatca tgatcgattt tttatttaga agggagtttc
     7501 tgcacattat gaatcagcgt cagcaatctt tgatctatca attaagtgag aacaataatt
     7561 atataacaac aaaaaagcta gcagagcatt tccatgtgtc agaaagaacg attcatagcg
     7621 atttgaatac cattgaaact tggttgagag acaaacatct gcctttaatg tttgaaagaa
     7681 aaaaaggtac aggtatcttg ttgaatggtc tgcctcacga aagggaacaa ctgaaacgag
     7741 cgcttaatga agagactcaa acagaaatgg ataaaaaaca gcttcgtcag ttattgattt
     7801 tccatctgct agtagcaaaa gacggattta gtcttgaaga actttcagaa aaactatatg
     7861 ttggaaaacg tacgattcgt aaagaattga cagagttgca aagctttttt gagtatcacc
     7921 atttacagct agtatccaaa acaaaactag gaacttttct taaaggcgat gaacaagaaa
     7981 aaagacagtt gcttgttaag acattacgaa atatgcagaa agaagatcca caaagtccgt
     8041 cgttgaaaga attcttcgct aaagatacat taaaagttat ccaacataca ttacaagatg
     8101 ttttttatga aaaccattta gaacagccga atggactggc tagtgtggag attcacatct
     8161 actttatgct cgagcgaatg aaacagtacc agaaagtgaa attaagtaaa gacgaaaacg
     8221 aagtcgtgga acacacccaa gcacaaaagt taagtagtca aatattagca aaactagcca
     8281 ccatctatcc aattgaattt tctcctgatg aaatcaatta tctggctttg cgtatcgcca
     8341 atctttttac aaattcacaa gcagccacac gttttcaaaa ggaatctagc gcacttgctg
     8401 atcatttgat cgaacaagtg gaacagtttc taggctattc attaaaagaa gatcagctac
     8461 taaaacaaaa tttaaggtct catttatctt caacttattt tcgactccat tatggtttgc
     8521 agatttctaa tccattgacg aaaaatgtgt ttagtacgta cacacaactt ttcttagttt
     8581 tacagttaat tttagaagat tattttgaaa aggaaaattt ttatgttcca caagaggaga
     8641 cagcttattt aacgatccat ttccaagcag cgatcgagcg acacaaacat cagaaaagta
     8701 gaagatatca aacggtttta gttagtgaat attcaaaagc tatggctact tttctcgaag
     8761 cacggctgaa cagagaactt ccagaattaa cgattatgga tctagttgaa tataagccag
     8821 aattaaagag acaagatttt ccctctgttg attttatctt ggctacgtta ccatttaaac
     8881 atataagcat tccagtaatc gagatctcac caatgatcac agaaactgat ctagcctatt
     8941 tgacaaaata tatgctggag catgtgccta tcaagaagaa aaaaacattt gatctagcaa
     9001 gctttactca tccatttctg atctttcctc aacttgaacg gacagaccca gccgatattt
     9061 taaactttat gggaaacgtc ttagtggaac atcattatgt ggagccggaa ttcatcgatt
     9121 ctgtcttgga aagagacagt cactccagta ctagagtagc accatttgta acaatccctc
     9181 acggtaatcc gctgtatgtg aagcattcga tgatttctat tgctacaatg aaagaaccag
     9241 tcctatggca cggggaacag atacgtatcg tactgctatt agcaataaag aaaaatgatt
     9301 tgaaacatgc agagttcaaa aaaatatttt ctgttattca ctatctagaa aagcagccag
     9361 aacaaatggc tcagttgatg caatccaatc atccgctaga aatcttaacg ttgtta
//
LOCUS       DAZAFF010000102         9388 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000102 DAZAFF010000000
VERSION     DAZAFF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.104"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..296
                     /locus_tag="ACG9BB_002471"
     CDS             102..296
                     /locus_tag="ACG9BB_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838346.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            565..864
                     /locus_tag="ACG9BB_002472"
     CDS             565..864
                     /locus_tag="ACG9BB_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838347.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(961..1395)
                     /locus_tag="ACG9BB_002473"
     CDS             complement(961..1395)
                     /locus_tag="ACG9BB_002473"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2838348.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1627..2337)
                     /locus_tag="ACG9BB_002474"
     CDS             complement(1627..2337)
                     /locus_tag="ACG9BB_002474"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFN2838349.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2327..3187)
                     /locus_tag="ACG9BB_002475"
     CDS             complement(2327..3187)
                     /locus_tag="ACG9BB_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFN2838350.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3192..3830)
                     /locus_tag="ACG9BB_002476"
     CDS             complement(3192..3830)
                     /locus_tag="ACG9BB_002476"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFN2838351.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3845..4144)
                     /locus_tag="ACG9BB_002477"
     CDS             complement(3845..4144)
                     /locus_tag="ACG9BB_002477"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2838352.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4187..5671)
                     /locus_tag="ACG9BB_002478"
     CDS             complement(4187..5671)
                     /locus_tag="ACG9BB_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFN2838353.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5691..6152)
                     /locus_tag="ACG9BB_002479"
     CDS             complement(5691..6152)
                     /locus_tag="ACG9BB_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2838354.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6170..7237)
                     /gene="ulaG"
                     /locus_tag="ACG9BB_002480"
     CDS             complement(6170..7237)
                     /gene="ulaG"
                     /locus_tag="ACG9BB_002480"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFN2838355.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7455..8225
                     /locus_tag="ACG9BB_002481"
     CDS             7455..8225
                     /locus_tag="ACG9BB_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN2838356.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8498..9325)
                     /locus_tag="ACG9BB_002482"
     CDS             complement(8498..9325)
                     /locus_tag="ACG9BB_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2838357.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2913 a         1927 c         1476 g         3072 t
ORIGIN      
        1 aattgattct ttttatttat cataaagata tagatacata atcattttca cttacatata
       61 tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat gtagaagtaa
      121 aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa tatcctttac
      181 ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa ataaagaacg
      241 agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa aagtaaatcc
      301 aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa gattatagaa
      361 ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt ataccatatg
      421 attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa gatgaatcaa
      481 atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt tttataatgt
      541 atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag tgaactactt
      601 aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca caaagtgaca
      661 gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc ggcaaaagga
      721 atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt tgctttaaaa
      781 aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt atttgataaa
      841 gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg atactaagtc
      901 tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg ttattatatg
      961 ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat aagaaatacc
     1021 acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg ttttctcagt
     1081 atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct ccaaagcttc
     1141 attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga ggatatcttg
     1201 aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa tcttcccttc
     1261 cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc ttgcagtgtc
     1321 ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat cgatcataag
     1381 ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa taatacagtt
     1441 ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg attccttaaa
     1501 attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt gataaatttt
     1561 cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac ccttacccta
     1621 gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat gtttatctag
     1681 taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt tggccatcag
     1741 acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat ccccccatgt
     1801 gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta attttctttc
     1861 cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc ttacttcttc
     1921 tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat gagtcgtgcc
     1981 ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg aatgagtatg
     2041 agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg ggagatctga
     2101 agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta ccaccatttg
     2161 atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga ctcctagctc
     2221 acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc gcggcaatgc
     2281 cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac aagacatcat
     2341 agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt tctcttcgaa
     2401 agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa tctagacgtt
     2461 tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga acatcacgaa
     2521 actggcctga atattctttt gttacagggt aagtatcttt taagtggata gcagtgatat
     2581 aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg gacaaattgc
     2641 ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt tctaaaaatt
     2701 ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg atgccatatt
     2761 tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg gaattcattg
     2821 atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg cttagattat
     2881 gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt tctggatcag
     2941 cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata cgtacaccgg
     3001 agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct aatcgtgcta
     3061 accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt tcttttacaa
     3121 gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa atcccaattt
     3181 gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg tgcggccttc
     3241 atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac atcgatccct
     3301 tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt aaagcctagt
     3361 tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc agataacagc
     3421 gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca atccatggct
     3481 tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc tttttgtgct
     3541 gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc tcctgctttt
     3601 gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc gactagtttt
     3661 ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg taagcaaaga
     3721 attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc tcctaatgca
     3781 tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat cttagttcct
     3841 tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg atttctttga
     3901 ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc tgagtaaaca
     3961 tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa ctttttccct
     4021 ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc attgcttttt
     4081 ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat actaaaattc
     4141 tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat actctctgct
     4201 tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga tttttccgat
     4261 attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca gcataaccta
     4321 ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac atcccgatat
     4381 aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg cctacttgta
     4441 tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg ttattggcat
     4501 aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg acgatcgttg
     4561 gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca ccaaacaaga
     4621 acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg tcaattcctg
     4681 gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt agttcatcga
     4741 cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt aaagaagtcg
     4801 tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga atcaaataat
     4861 ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac gaagtagcaa
     4921 ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca atttttttgt
     4981 cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca gctaattttg
     5041 caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca ccttctgtca
     5101 attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa cctaaaatca
     5161 gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac agtaaaatcc
     5221 aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg gaacgcagtt
     5281 tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc agtaataaaa
     5341 tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc acagcatttt
     5401 gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt agtccaacca
     5461 agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata aagtaaccga
     5521 ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc aaaatatagc
     5581 ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt gttgcaaaat
     5641 acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa ttaattatat
     5701 tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg ctcaaccaat
     5761 ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc ttctggatct
     5821 tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc ttcactttca
     5881 aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac accatcactt
     5941 ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg accgtatttt
     6001 tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt atcgatcaat
     6061 acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc tgggtaatga
     6121 atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct tataaaaacg
     6181 acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga taaaaattaa
     6241 actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc cagatataag
     6301 gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg atctcttttg
     6361 gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact tttgcattca
     6421 atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc cctcgtgggt
     6481 tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt ttggcaaata
     6541 aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt tcgaataaat
     6601 agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc tcttctggat
     6661 cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca ttgatgtctt
     6721 tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga attccccatt
     6781 tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga caattttgat
     6841 gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta acgattaaag
     6901 cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt aaattaggct
     6961 gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc atgccattcc
     7021 catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt ccttcatgac
     7081 ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca ggatccacta
     7141 cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt tctactaacc
     7201 acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc ccatatcgtt
     7261 gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc ttttatatga
     7321 ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt ataataaaga
     7381 cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa gatgtccctc
     7441 aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa aaagaaattt
     7501 tggaactgct tcagcagact gaacaactat caactttcga attagctgaa tcccttgatg
     7561 tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac gagatcatac
     7621 gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat caatctggtc
     7681 ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac gattatgata
     7741 cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt caatctaacc
     7801 atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa gataattgta
     7861 gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg ggcgacatcg
     7921 cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt agcggagtcg
     7981 atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac gaaatgatgg
     8041 tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc ggactgacat
     8101 caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat aaattttgtt
     8161 ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa gtagattcat
     8221 tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat aacaaattta
     8281 tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga tgtatccatc
     8341 caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg cctaaatagg
     8401 aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt tttcgaataa
     8461 acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg ttttcaatca
     8521 cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc atatatcttt
     8581 ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa agcaagtatt
     8641 ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca cataattgga
     8701 ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa tatacaatgc
     8761 agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc tccacataaa
     8821 ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt gcagctgctt
     8881 gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa taaaataatg
     8941 ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat aaaatttgat
     9001 ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt ttataagatt
     9061 cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc ctgattttgt
     9121 cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg caaagcgcac
     9181 ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca gttgcttgtt
     9241 cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg tattctttta
     9301 gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat tatttctcct
     9361 ttatatcata cacaaaaaaa agttctaa
//
LOCUS       DAZAFF010000103         9254 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000103 DAZAFF010000000
VERSION     DAZAFF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.105"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..798)
                     /locus_tag="ACG9BB_002483"
     CDS             complement(<1..798)
                     /locus_tag="ACG9BB_002483"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838358.1"
                     /translation="MSENKKKWLLIHPFDYISRTNILEEILTENSIEYFNVVSDYSHI
                     RKQFLSSKERHMIKNRHVIKTIGYKKNISVMRILSHIWFSIKTLLFIRKEKPEIIYCR
                     IPPNTLVFFIAKFSRCNRVIFDVYDLWPESMPGIGNKYKFFTDLWKKLRDQYLSNSSL
                     VITECDYFKKFIKISGESKNMTIYPCSFNVDDQNDYGEKIVNKSLESTLNLAYLGTIN
                     NIVDLEAINELLKTCKRKFGNKINLYFIGKGESKTKLADICEKNHIEF"
     gene            complement(791..1420)
                     /locus_tag="ACG9BB_002484"
     CDS             complement(791..1420)
                     /locus_tag="ACG9BB_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFN2838359.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(1413..2531)
                     /locus_tag="ACG9BB_002485"
     CDS             complement(1413..2531)
                     /locus_tag="ACG9BB_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFN2838360.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(2533..3165)
                     /locus_tag="ACG9BB_002486"
     CDS             complement(2533..3165)
                     /locus_tag="ACG9BB_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFN2838361.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(3176..4993)
                     /locus_tag="ACG9BB_002487"
     CDS             complement(3176..4993)
                     /locus_tag="ACG9BB_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFN2838362.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(5006..5770)
                     /locus_tag="ACG9BB_002488"
     CDS             complement(5006..5770)
                     /locus_tag="ACG9BB_002488"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFN2838363.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5798..6496)
                     /locus_tag="ACG9BB_002489"
     CDS             complement(5798..6496)
                     /locus_tag="ACG9BB_002489"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFN2838364.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(6508..7287)
                     /locus_tag="ACG9BB_002490"
     CDS             complement(6508..7287)
                     /locus_tag="ACG9BB_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFN2838365.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(7303..8247)
                     /locus_tag="ACG9BB_002491"
     CDS             complement(7303..8247)
                     /locus_tag="ACG9BB_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFN2838366.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(8293..9072)
                     /locus_tag="ACG9BB_002492"
     CDS             complement(8293..9072)
                     /locus_tag="ACG9BB_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838367.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2537 a         1883 c         1535 g         3299 t
ORIGIN      
        1 aaattcaata tgattttttt cacaaatatc agctaattta gttttgcttt caccttttcc
       61 aataaagtaa agatttattt tgtttccaaa ttttcttttg catgttttca ataattcatt
      121 aatcgcttct aaatctacga tattattaat cgtacctaaa taagcaaggt tcaacgtaga
      181 ctctaagctt ttattgacta ttttctctcc ataatcattt tggtcatcta cattgaaaga
      241 acatggataa atggtcatat tttttgattc tccggaaatt tttatgaatt ttttgaaata
      301 atcgcattct gttataacta gactagaatt actgagatat tgatctcgta gctttttcca
      361 taaatcagta aaaaatttat atttgttccc tattcccggc attgactctg gccataaatc
      421 atacacatca aaaataactc gattgcatct actaaactta gcaataaaaa aaactaaggt
      481 attaggtggt attctacaat aaataatttc aggcttttct tttcgaataa ataataatgt
      541 ttttattgaa aaccatatat gagataaaat tcgcattacg gatatatttt ttttatatcc
      601 gatcgttttt atcacatgac gatttttaat catatgcctt tctttactgg atagaaattg
      661 ttttcttata tgtgagtaat cagatacaac attaaaatat tcgatactgt tttccgtcag
      721 aatttcctct aatatattcg ttcgactgat ataatcaaaa ggatgaataa gcagccactt
      781 ttttttattc tcactcatct atcttcgcac cctcaaattt ctcagtagtc ccacttgcta
      841 ctgatgttat tccatcacgg acaataactt tctttagcgt taaaaaaaat atttttatgt
      901 ctttcatcat tgaaatgttc tccacatatt caatatcttt ttcaaatttt tctttccaac
      961 taattgcatt acgaccactg acttgagcta atccggttaa acctgggcgc acaaaatgtc
     1021 tttttcgttg ttcattactg tatagtgaaa gatactcaac taacagagga cgcggtccaa
     1081 taatactcat atctcctttt aaaatattcc ataattctgg tagttcatca agactagtcg
     1141 aacgcaatat tttaccaaat tgagtcaatc tgatatcgtc tgataataaa ttaccttctt
     1201 catctctttg atctgtcatt gttcgaaatt tatacatcag aaaaatcttt tcatgttttc
     1261 caggtcgttg ttgtttaaag atcactggcg atccaagctt tattcgaact aggattgcaa
     1321 caaacgtcat cagtggccat aacattatta aacctaaaat tgaaaaaaga atatctatca
     1381 aacgtttaaa gaacttttcg tagatacctc tattagacat tttgcatcac tcgcttaatt
     1441 gtattagtta ctcgatacaa atcatcatcg gtcattttag aatctgaagg taaacagata
     1501 cctttttcaa acaatgtctg agcaacatct gtaccaatat actcatactt atcgaaaact
     1561 ggctgtaaat gcattggttt ccatatcggg cgggcttcta tattattttt atctaactca
     1621 tcaataagat gattcgtatt tattcccttt actgttatga cacttaacca ataatttggt
     1681 tcattccaat tattttctgg catgaattgt atatttgaca atttagacaa ttctttttta
     1741 taaaaactat gaatatatcg ttttttctca attctttcat ttaaaacttt caattgtcct
     1801 cgaccgatac ctgcaacgat attgctcatt cgataattat agccaatttc attgtgttga
     1861 taatggcgtg ccttctcacg agattgcgta gcccaaaacc tcactttttc caccttttct
     1921 ttgtcattag acacaagcat tccgccacct gaagtcgtga ttatcttatt tccattaaaa
     1981 gaataaattc catagtcgcc gaatgtacca gtatattttc ctttatatgt tgttcctaat
     2041 gattctgcag catcttcaat taaaacaaca ttatgttctt tgcatatttt agtaatttca
     2101 tctagttcag ctgataaacc gtacaaatgc actactaata cagcttttgc gttaggatat
     2161 ttttctagcg ctgttcgcaa atgttcaggg ttcatattcc aagtttcgta atcactatca
     2221 acaaatactg gaatagctcc ttgatagatg attggattgg cagtagctga gaacgtcaaa
     2281 gactgacaaa tcactatatc atctttaccg accccagccg cttttaaagc tagatgtata
     2341 gctgctgttc cagaagatag tgcagctgca tgactagcac cgacatattc tgctaattcc
     2401 tcttcaaaac cattgacatt catacccaaa ggagccaccc aatttgtatc aaatgcttct
     2461 tttacatact cttgttcata tccttcatca ctcatatgag gagatgctaa taatattcgt
     2521 ttattttcca acttaatccc atctttctat gactttggta ggtacgccaa catttttagt
     2581 cttattatta atatttttta ttactacact tcccgcacct ataatgttca tatttccaat
     2641 tcttagattt tgtattattt tagtacctac tccaatttca tttttatctc ctaatagaac
     2701 atgtcctgaa atatttacac ttggaaaaat tgaattataa ttaccgattt gagtatcatg
     2761 tccgatagta gagccgatat tgatcatatt gaaatttcct aaaactacgt cagcagtgta
     2821 agtcgtatag ggcatcaaaa tattgccaat tcctaactga acatttatgc ccactagtgc
     2881 acttgatgca actaaattag ggaaaatcaa tttattattt ttatttatct tttgatatat
     2941 tttctctttg atatctggcg aagcgattcc cagaaaaata gcagtttctt cttctaattc
     3001 taataacgag tctattgttc ccaatacagt ttttcccact aattcttgat tttgcatttt
     3061 taacgaatca tcgacaaaac caatcaattc atatctagag aaactttcta gtagaaaagc
     3121 aatctctttg ccaaatccac cagctcctac aataattgct ttcactttac tccccctact
     3181 tattcgaagc attcgcaaaa gtgacaatgt ctttagccaa ttgttcatcg tcttgcggca
     3241 ggctcttgac aaattccatc actgtttgca tggaataacc tttgatgttt cccacaaaga
     3301 tcttctcgta aactgcttca tcgtttcttt ccttatctaa caatagttct tcatatagtt
     3361 tttcccctgg acggatacct gtttcaatga tttcgatctc gtcttcgcta tatccgctca
     3421 atcggatcat gttttttgct agatctacga ttttcactgg ttcactcata tctaaaataa
     3481 agatttcgcc gccttttgct aacgccccag attgaataac taaacgactt gcttcaggaa
     3541 tcgtcatgaa atatcgggtc atacgaaagt ctgttacagt gactggccct ccttgagcaa
     3601 tttgttcccg gaatactgga atcacactac cgcgagaacc aaggacattc ccaaatctga
     3661 cagctgagaa tttcgtacat ccttcttcat tcaacccagt cacgatcatt tcagcgattc
     3721 gttttgttga acccatgacg tttggcggat tgttggcttt atctgtcgag accatgacga
     3781 agttttttac tttcgctgct ttggctgctt ctgctacatt ttttgtaccg aaaatattgt
     3841 ttttgactgc ttcttttggg ttatattcca tcaatggtac atgcttatga gcggctgcat
     3901 ggtagacgat atctgggtgg tattgttcca tgatctcatt gattttcttg cgatcttgga
     3961 tatccgcaat gatcggcacg atttctgtag gacacttttg atgatgcgtg cgtaattcac
     4021 ggtcaatcaa gtaaatcgag ttctccccat gacctaaaag taatagtttt gcagggtcaa
     4081 attggatgat ttgccggcag atttctgaac cgattgatcc tcctgcaccg gtgacaagaa
     4141 tgactttgtt tgtgatctgg tctttgatgg attcgatatc caactctacc tcatctcttc
     4201 ccagtaattc cactacgtcg atggttttta attttgatac gctgattttc cctgaggcaa
     4261 gttcttccat agaaggcatc gtattgactt tcacatgagc agattcgact aactcgaaga
     4321 ttcttcgtaa tttttttctt gataaagaag ggatagcgat cgttaccatt tggatgtcat
     4381 atttttcgat caattccggt atatctttta atgaccccag tacttttttt cctgaaagat
     4441 aggtatttct tttattcgga tcatcatcca cgaatccaat gacatggata tcttgagccg
     4501 ttttagaacc aagaaagctg ttgtataaaa tacgtccacc ttcgccggcc ccaacgatca
     4561 aggtattttt agcggaatct gttgatagat acctcatatt ttttgtttcg atatagatac
     4621 gccagatcaa tcggctgcca atgatcagta atagcgaaag taggtatgca aagatcacta
     4681 aacgtaaact gtattgttta tttatgaaaa gcaatatgat gatacttgag gccgcacttg
     4741 cagataacga accaaaaatc gcaatgatct ctcttaaatt tgtataacga ttgattcgag
     4801 taaaaacttt gaataaacta ccatagaata aataaaaacc aatcgacaaa ccgatagaga
     4861 ttaaaataag gtccattggt atagctacaa acggcttcat gaatttagct gctgcaatat
     4921 tcgcagcaat caacaacaag ctgtccacaa ataccaatgt ggcaattttt cttcttcttg
     4981 ttaatacaaa caccctacat tccccctaaa ataaaccaaa cttcttcttt tttacctctt
     5041 catagactgg ataactgatc tcatccccgt tgatcaggtc ctttgcgact tgtttcatct
     5101 gtgctacttt ttccttacca aagtccttgg cgatttgctc ataacattct tttaagtaaa
     5161 aggtacgttt agagacccca tgggcatcgg aagcagccat ttgcacgagg ttatgtttga
     5221 ccatcatttt ggctgttttc tggatgtctt ttccaaatac accgacataa cttggtgcgg
     5281 tgagttgagc caagcatccc atatctagga atggcagcaa atgatttggg tcttctcgaa
     5341 aatgcgcgtt gcgttctgga tggacgatca ctggtgtctt tcctagttgt aacaactcaa
     5401 aaaagagcca ttctgcatat aacggcactt ccagtgtcgg aaattcgatt agaagatatg
     5461 tatcatctaa atctgtaaat aaaatttcat ctcggtggat gtcttcaatc agtgttccgg
     5521 taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ttgtcttttc tctaattctg
     5581 cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat tttccattat
     5641 tgtgatgggg tgtgcataaa atatgtgtga tcccttgttg aatcgctttt tcagccatgg
     5701 cgatactggc atctatgttt tctgctccgt catcgattcc tggcaggata tggcaatgta
     5761 gatcgatcat ttctcttgtc tcctctcttt ttatttctta attcccgtag taatagccag
     5821 catctctgct gtgttctgct ccattgtata caacaccaag gactcgcgct tgtaccatat
     5881 ctaacagttc ttttgcttta gtcaatgatt cttttcgtga cgtattttcc cgtacgacca
     5941 ataaggtgcc atccgttttt gaagacatga tctgtgcatc tgttacggca acgactggtg
     6001 gcatgtcgaa aatcactaca tcaaatagtt gtcttgcttc tgctaaaatc tgatccattc
     6061 ttggcgaact tagtagttct gatggatttg gtggtttagg tccacttggc agaatcgaca
     6121 aattgtcgac tggtgttcgt tggatctcat ctgctacact tcctgatgaa ctaagaacag
     6181 tacttaatcc gcttgcgttg ttcaacttga acgttttata gatgacgggc ttacgtaagt
     6241 ctgcatcgac tagcagtacc cgttgaccag atttggcaaa aacaacggca atgtttgctg
     6301 cagtggtgga ttttccttct ccaggacctg atgaagtcac gacaatcgtt ttgatttgct
     6361 gaccagctgc ggaggcaaac tggatattcg tccggatcgt acgatattgt tcggcgattg
     6421 gtgaagatgg gtttgttaat gtgatcaaac tgactgcatg agtttgagtt gctttttgtt
     6481 tctgtgttcg tgccattttt cttcctccta gactcttgag cggcttctgc gtgatggggc
     6541 tgttttatcg tttgtagctt ttggtcgtgc aggagctgat ccttttttac ggataccagc
     6601 gttcaattct tttgctgaca tttctggaac tgttcctaag attgggaatc ctaatgtatc
     6661 cgtcacatat ttttcatctt tcactgtccg atcaagtaat tcgaataaga atgccagacc
     6721 tacgcctacc atcattccta acactaagcc aatcactaga ttcaatttat tatttggcga
     6781 aacaggtgtc gtattggcaa ctgcacctga aataatcgag attttatcga cattcatcac
     6841 gtctttggcg ttttcttgga aaacttgtga agttgtatta gcgatctgtt gagcagtcac
     6901 tgcattcgtg cttattgcct ggatcgagaa catttgtgaa ttttgtgact gattgacgga
     6961 gatcgcatct ttgatttgtc cagcagtcat tttcacattg tcttctgttt ctaaacgatc
     7021 tttgacttca tttagtacta aatcactgac gatcatatct ttatacgtat tgatcatttg
     7081 caggttcgta ttgacatcat tgacatttgc tgtgtctgat tgtggcagtg tcacgatcaa
     7141 ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc ctgagatggc
     7201 caagccagca aacattgaga tgatgattgt taccaagtgt ttgcgtaatg tttcaaaaat
     7261 ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat tctctagatt
     7321 catttgatct tttaattctt ttttcacacg atcaagttct tgatcgctga ctttttgata
     7381 ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttga tcgtaccaaa
     7441 tgcctcccga taatctaaag cgattttttt catttcatca aagctcatgt ctgttttcat
     7501 attcgactct agagcattca aaacggattg atagttgctg acaccgttta aactcaatac
     7561 tttttttgct acaccttcta cgatctttct ttgacgctct tgtctaccat agtcaccatt
     7621 tggatcttca taacgcatac gagaataaga taatgcctga tccccgttca atgtaatttt
     7681 accgattgga aagtcatagc catcttgtga gaaagtgagg tcattattga cttcgatacc
     7741 accaactgca tcaacgagtt cctttagccc tttcatattg atagagacat agtgatcgat
     7801 tgggatgtct acatattttt gtagggtatc catcgccatg gcagctccgc cgaatgcgta
     7861 agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg tatctcgagg
     7921 aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca tcatcgtatc
     7981 tgatcttcct tgatcgaccc gacctaaatc tccagtatca atgcccatca atagaacaga
     8041 gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg ttcgttccac
     8101 tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg ctcctactcc
     8161 tacaattgct aatactaata cgataagaac tgctaaaata atcagaaata tttttttccc
     8221 tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt gcttgaatca
     8281 acgttttaac tgtcaataac ttatattgct atagcccatt tgctgagcaa cagtttgtgc
     8341 ttttgttgcg tatgctgcgc ttattttttc attccggatc tgctctattt gactgagcgc
     8401 cgtcaaatat tgatcgtaag tagcggtggc agtcacttga ccatccttca acatcgtatc
     8461 gaataactga tctagttgcg tgacttgcgc gatctgagta tcagcgaatc ctagatattg
     8521 cataacaacc gttttccaag gactatcttt aaaaaagctc atattttcac gtacacgagc
     8581 gacatctgca cttgtcacct tttctttgat gacgacatcg tctacagctt gctgccagtt
     8641 tgatacattt ttagtaaata aatcgttgac agctgtctgg atcttcagtt ttgattctgc
     8701 actatctaac tgtgtcacga ttgctttctt ctcatcttgg attgcaacag cttctttagg
     8761 caattctgat tctttgatgt cgtaatcatc tgctgaaagt ttgacagagg atagcttatt
     8821 tgacaaactt gtgattgttt cttctgttat ttcagggttt agaaaatttt tttcttttgt
     8881 actatacagc ttgtctactt cttttgataa agaagtcaag gtgtcttttt gattggtgat
     8941 ggcagattct gcataattga tccgttgctg atgttgccgc tgattattga tatggtaacc
     9001 aaacaaacag atcgcaatag cacctgcacc taccgccaca gcagtcaatg acttgacgaa
     9061 tggcttttcc atagattcac tcctaagtat tgatgataga taaattaaga tataattcac
     9121 atacataaaa atgacagctt tttattttaa aaaacataat taaattaata tattcacaga
     9181 aatatttgta aacaagtttt ccctatataa tcataccaca aattgttttc ttgactaggg
     9241 caatttacga ttta
//
LOCUS       DAZAFF010000104         9149 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000104 DAZAFF010000000
VERSION     DAZAFF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.106"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG9BB_002493"
     CDS             6..305
                     /locus_tag="ACG9BB_002493"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFN2838368.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG9BB_002494"
     CDS             378..1739
                     /locus_tag="ACG9BB_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFN2838369.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG9BB_002495"
     CDS             1761..2021
                     /locus_tag="ACG9BB_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838370.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG9BB_002496"
     CDS             2035..2787
                     /locus_tag="ACG9BB_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFN2838371.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG9BB_002497"
     CDS             complement(2881..3486)
                     /locus_tag="ACG9BB_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN2838372.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG9BB_002498"
     CDS             complement(3539..4513)
                     /locus_tag="ACG9BB_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFN2838373.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG9BB_002499"
     CDS             complement(4891..5610)
                     /locus_tag="ACG9BB_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2838374.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG9BB_002500"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG9BB_002500"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN2838375.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG9BB_002501"
     CDS             7161..8558
                     /locus_tag="ACG9BB_002501"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFN2838376.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG9BB_002502"
     CDS             8639..9079
                     /locus_tag="ACG9BB_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN2838377.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
BASE COUNT         2886 a         1645 c         1897 g         2721 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtaaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgt
//
LOCUS       DAZAFF010000105         8942 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000105 DAZAFF010000000
VERSION     DAZAFF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.107"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..1154)
                     /locus_tag="ACG9BB_002503"
     CDS             complement(15..1154)
                     /locus_tag="ACG9BB_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFN2838378.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1553..2851)
                     /gene="eno"
                     /locus_tag="ACG9BB_002504"
     CDS             complement(1553..2851)
                     /gene="eno"
                     /locus_tag="ACG9BB_002504"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFN2838379.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2967..3722)
                     /gene="tpiA"
                     /locus_tag="ACG9BB_002505"
     CDS             complement(2967..3722)
                     /gene="tpiA"
                     /locus_tag="ACG9BB_002505"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFN2838380.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3826..5016)
                     /gene="pgk"
                     /locus_tag="ACG9BB_002506"
     CDS             complement(3826..5016)
                     /gene="pgk"
                     /locus_tag="ACG9BB_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFN2838381.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5126..6127)
                     /gene="gap"
                     /locus_tag="ACG9BB_002507"
     CDS             complement(5126..6127)
                     /gene="gap"
                     /locus_tag="ACG9BB_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFN2838382.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6168..7259)
                     /locus_tag="ACG9BB_002508"
     CDS             complement(6168..7259)
                     /locus_tag="ACG9BB_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFN2838383.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="ACG9BB_002509"
     CDS             complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="ACG9BB_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFN2838384.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8802..8873
                     /locus_tag="ACG9BB_002510"
     tRNA            8802..8873
                     /locus_tag="ACG9BB_002510"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8835..8837,aa:Arg,seq:ccg)
BASE COUNT         2317 a         1897 c         1647 g         3081 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttattt atacgagagg acttgcccat agattcctat
     1321 tctactgttc tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt
     1381 caaggatgat cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa
     1441 aaaaactctc tacctgctga tatttcagca agtagagagt tcgattactt aactaagtga
     1501 tcatactcga ctatctttca atagtcgatc atgtatcttc attagaaaag aattatttgt
     1561 tttttaagtt gtagaaagat ttcaaacctt tgtattctgc aacttcacca agttggtctt
     1621 cgatacgtag taattggttg tatttagcaa tacggtcagt acgtgataga gaaccagttt
     1681 tgatttggcc agcgtttgtt gcaacagcga tatcagagat tgttgaatct tctgtttcac
     1741 cagaacggtg agatacaact gctgtgtagc cagcttcttt agccatttcg atagcttcga
     1801 aagtttctgt taatgtaccg atttggttca ctttgataag gattgagtta gcaattcctt
     1861 tttcgatacc ttcagataat ttttgagtgt ttgttacgaa caagtcgtca ccaactaatt
     1921 gaactttgtc gcctaatacg tcagttaatt tcttgaatcc gtcccagtcg ttttcgtcta
     1981 atccatcttc gattgagatg attggatatt tagaaactaa ttcttcgtag aatttgatca
     2041 tttcgtcagt tgttttttcg ccttcgcctg agtcagctag tacgtaaaca cctttttctt
     2101 tgtcgtagaa ttctgaagaa gcagcatcca tagcaagaac aacgtcttta ccaggtacat
     2161 agccagcttt ttcgattgct tcgatgatta cttcaaaacc ttcttcgttt gaaccaaggt
     2221 ttggagcaaa tccaccttcg tcaccaacag aagttgctaa tccgcgagct ttcaagattg
     2281 aagctaatgc gtggaatact tcagcaccca tacgtagagc ttctttgaat gttggagcgc
     2341 caacaggcat gatcatgaat tcttggaagt cgattgagtt atcagcatgt gatccgccgt
     2401 tgatgatgtt catcattggt gttggcaata cttttgtgtt gaatccgcct aggtagtggt
     2461 ataaaggtac ttctaggtag tcagcagctg cacgtgcaac agcgatagaa acacctaaaa
     2521 tagcgttcgc acctaattta cctttgttag gagttccgtc taaagcgatc atagctttgt
     2581 cgatagccat ttggtcacga acatcgtagc caatgattgc ttcagcaatg atgttgttta
     2641 cgttgtcgac agctttagtt acacctttac cgccataacg agctttgtct ccgtcacgta
     2701 attcaactgc ttcgtattca ccagttgaag cacctgaagg aaccattccg cggccaaaag
     2761 ctccgctttc tgtgtatact tctacttcga ttgttgggtt accacgtgag tctaagatct
     2821 cgcgagcgta aacatcagta ataattgaca tgttttgtct ctcctttgag tttgtttttt
     2881 cctaagggat ttccctcata tacgatttta gttaattttg ctcattagtg caaacatttc
     2941 ttgcattttt cgcaaatttt caacaattat tttacagcgt ctaacaatga caagaatgaa
     3001 tctgcttgca agcttgcgcc accgactaaa gcaccgtcaa cattttcttt tgccatgtat
     3061 tcagcaatat tttctggttt tacagaaccg ccgtattgga tacgaactgc ttctgatact
     3121 tcttttccat aaagtttttc aactgtttga cgtacaactc cacaaatttc gtcagcgatt
     3181 tgtgcgtcag ctgatttacc agtaccgatt gcccagattg gttcataagc aataaccatg
     3241 tttgacacct gttcagctga aagatcagct aaaccagctg taatttgtcc ttcgatccat
     3301 tcagcagttt tgcctgcttc ataggtttct aaagattcgc cgcagcatag gattggtgtc
     3361 ataccatttg caaagatagc tttcgctttt ttattgatgt cttcgtctgt ttcatggaaa
     3421 tattcacgac gttctgaatg tccgatgatc acataatgaa cacctagatc agcaagagct
     3481 gctggtgaag tttcaccagt gaacgcacct gagttttccc agtagcagtt ttgtgctgag
     3541 atttttagtt ctgtaccttc agctgatgtc attaattctt gtaagaataa tgcaggagat
     3601 ccgatgactg aatcaacttt gtcatttgaa gggattttgt tttttactgc ttcagcaaat
     3661 tcttttgctt ctgatgcagt tttgttcatt ttccagttac ctgcgataat aggtttacgc
     3721 attgaggaac acactcctta atttattgaa aaatggctag ggagtcatga aaactccctt
     3781 caccttttgt tcgattactc gtcagaattg agcaaacttg gtcgtttatt tatcgttgat
     3841 tgctgctagt cctggtaatt ctttaccttc aagcaattcc aagcttgcgc cgccacctgt
     3901 tgagatgtgt gtgaatttgt cagcaaatcc taattgttct gctgctgcgg ctgagtctcc
     3961 gccaccgatg attgttgttg cgccttctaa gttagcgatt gcttcacaaa caccgattgt
     4021 acctttagca aagttactca tttcaaatac acccataggt ccgttccaaa caactgtttt
     4081 tgcaccttgt aatgtatcag caaataattt tactgatgct gggccgatgt ctaaagccat
     4141 caaaccatca ggaatgtctc cttcgattac ttgtgtttca acatcatttg aaaattctgg
     4201 tgcacagaca ttgtcgattg gcaagacaag tttgtcgcct gctttttcga ttaattcttt
     4261 tgctagttcc actttatcag cttctactag agagttaccg atcttgattc cttttgcttc
     4321 gtagaatgtg taagtcattc cgccaccgat caagatttta tctgctttag gaatcaagtt
     4381 ttcgattacg ccgattttat cagaaacttt tgcgccacca aggattgcaa tcattggtcg
     4441 ttttggttct tctactgctt caccgatgaa cttgatttct ttttccatca agaatccagc
     4501 aactgttggg ataccagttg aagcgatacc tacgttagaa gcatgtgcac ggtgagctgt
     4561 accgaaagca tcgttaacaa atacatcgcc taatgaagcc cagtatttac ctagttctgt
     4621 atcatttttg ctttcttttt taccgtcaat atcttcaaat cgtgtatttt cgaatacgac
     4681 aacgtcgccg tcattcatgt tgcggattgc ttcttctaat tgttctccgc gagtttcagg
     4741 tacgaatgtt acttctttac ctaataattc gcctagacgt tttgcaactg gagctaaaga
     4801 ttttccttct ttatcttctt ctgtttttac acgtccaagg tgagagaaaa ggattgcttt
     4861 tccgccttgt tctagaacgt agttgattgt tggcaatgct gctttgatac gagtgtcgtc
     4921 agtgatcacg ccgtctttca aaggaacgtt gaagtcaaca cggacaagaa cttttttgtc
     4981 ttttaaatcg atatctttca ctgttttctt agccatttta tttcctcctg aataaactta
     5041 ttatcaaaaa aagcggaaga agcgcgattg cttccccgct tttaaatgta catgtctata
     5101 atttacactt gtacgtttgt ctatcttata agttagcgaa gtattctaaa gtacgtacta
     5161 attgtgcagt gtatgacatt tcgttgtcgt accaagcaac agttttaact aattgtttgt
     5221 cgccaactgt cattacttta gtttgtgtag catcaaataa tgaaccgtaa gtcataccaa
     5281 cgatatctga agaaacgatt tcgtctgtgt tgtatccgta agattcgttt gaagcttctt
     5341 tcattgctgc attgatttcg tcaacagtta cttctttttc aagaactgtt actaattctg
     5401 ttaatgaacc tgttggtaca ggaacacgtt gagcagctcc gtctaattta ccgttcaatt
     5461 cgggaattac taaaccgata gctttagcag cacctgttga gttaggaacg atgtttgcag
     5521 cagcagcgcg tgcacggcgg aagtcacctt taggatgagg tccgtctaga gtcatttggt
     5581 cacctgtgta agcgtggata gttgtcatta atccttcaac aacaccaaat ttgtcgttca
     5641 atgttttagc cataggagct aaacagttag tagtacatga agcacctgag ataactgttt
     5701 ctttaccagt taatgtttcg tggtttgtgt tataaacgat tgttggaaca tcgtttccgc
     5761 caggagcaga gataacaacg cgtttagcac cagctgttaa atgtttttca gctgctgatt
     5821 tagaagtgaa gaaaccagta cattctagaa cgatatctac gcctagttcg ccccatggta
     5881 attcttctgg gttgcggtta gctaatactt taacttcttt tccgtttacg ttgaatgaac
     5941 cttcatgaac ttcaactgtt ccgttgaaac gtccttgagt tgtgtcgtat tttaacaagt
     6001 gtgccaacat tttagcatct gtcaagtcgt tgattgctac tacttcgatt ccatctacat
     6061 cttggatacg acggaatgct agacgtccaa tacgtccaaa accattaata cctactttaa
     6121 ctgtcattta agatttcctc cttatgaaaa tcaaaaaaat atttatttta aagggttacc
     6181 ccttttaaaa tctcatttgc agcagcttca tcagtgatga gccacgtttg ttttggtgca
     6241 tttttcatat aagctcgaat tgctttagct tttgattttc cgcccgcgat tgccatgatg
     6301 atcgggactt tttccaaatc ttgcaattct atgccgattc tagggacctt ataaacgact
     6361 tcaccttttt cattgaaaaa gtagccaaaa gattccgcaa cagcatttgc ttgtttcagc
     6421 atgactaatt ctttttcagt catcttacga cgtgctgcca tgtgtaatgc acgcccaata
     6481 ctatgaataa cacaattcgc ttcactaatc aagtttaaga cttcttggat agatggctca
     6541 ttgagtaaag aattgtaagt tgccagactt aattgttccg gaacataaag tgcgcggtgg
     6601 ttaccgtttg ctttagcagc cattcttgca ctgacagaat ttgcctgtac agttatcgct
     6661 tcaccaatcc cgcctcttgc agggacaaac aggttatgcc gtttcttatt ttccagatta
     6721 gacaattgtt cggctaccac agccatagta gttcctccca tcacagcgat gatattctcg
     6781 ccttctggaa gtagtcggtc taaggcttcg ctcagcgctt cgccgaatgc atcagccact
     6841 ttggtctgct cttcgctgtt tcctgaaaca acgatacagc gttggttacc aaaatattct
     6901 gccaactgtt gttcggtttg atgcgtacct aaaagctgat ccatgaagtt ttcaaggctt
     6961 tgataaactt cttcgccctt agttgtcagc gtcataccgc tttttgaggt gtcgatcaaa
     7021 ttcagatttt tcaaaaggtc tgtttctgtt cgtaagactc gttctgtgat ccccatactt
     7081 tccgataaac ttcgacgccc aatcggctgc atccaataga tatttcgtaa aattttatag
     7141 cgttcttgca acacttcgat aatatctggt gcaacagatt caatcatttt tatttcatcc
     7201 agcatagcga tctccttagt tggacacttt acgtccaatg tagacttaaa acgacccata
     7261 tacgataaaa aaagaaatta actattcgaa tttcacatca ataaagggaa atctcaacct
     7321 cacgactgta agtctaacag ttttgctctc gtttttcaac taaaaagact agaaaaagtg
     7381 aaatactttt tctagtcttt tgactgatta gttatcataa cgttttcttt ttgatgaact
     7441 cgggattcct aataaatcac gatattttgc tactgttcgt cttgagatcg ctatctcatc
     7501 ttttttcagc aattcaacca atttctggtc agacaacggc ttgtttttgt cttcttgatc
     7561 gactaattcc tgcagctttt tcttcgctgt atctgctgaa aggtcttctg tctgttctgt
     7621 actattcgta ggaattcgtg tagtgaaaaa tttttttaat tcaaatacac cgaagtctgt
     7681 ttccaaatat ttcccgttga ctgcacgact gaccgtggat tcatgcacat ctatctcagt
     7741 tgcaacttcc tttaacgtaa gaggcttgat tggacgttcc ttgtcgataa agaaagcttt
     7801 ttgccgggaa acaatgactt gagcaacccg caaaattgta tccccacgtt gtaggatcgt
     7861 tttcttcaac cattcgaatt cctgtttccg ttcttttaaa taattttcag tttctttatc
     7921 tgcttgttgt gacatttgtt tgaagtaact ctcttgaaag cgtatatttg gctgattttt
     7981 tcggtttgag ataacttgta catttttatc gtcgtccact aatacccgga catcaggaat
     8041 aatgtacaat tcgctgctac ggtcgaaaat ccggccaggg cttggactca acgtttgtaa
     8101 atagtcgaat atctgctgaa cagcatgtag atcaacagaa aaacgttgtg cgatttcttt
     8161 ccattttcgc tccaccagtt cctcaaaaca ttcttccaac acgatataag ctaattccgg
     8221 cgcataatca tctcgttctg tttgaagcat gagacattct tgcaaactac gtgcacctac
     8281 tccagctggt tctaattgct gaatcagagt caacgcatcg agcatttgga taggcgtagc
     8341 tccaatctct tttccggctt cttctaaact aatcgtcaaa aatccgttca aatcgatgta
     8401 ttctacaaga taaagaacga ttttcctcaa aaaagtatct cggtaattca aatggacttg
     8461 ttcgatcaat gaatcaaata aagacccttt tgtatcagga atctgattta ggtaatttgt
     8521 ctcttctcct tcataagaag atgaagaagc ttttatgtaa tctggctgcc attccggctc
     8581 aacaacttcc accaatggat tttccattgc ttgattctca acgaatgcca acagctcctc
     8641 actgttatat tgtaaaactt ggatggactg ttgcaactgt tgcgtcatgg ctagtttctg
     8701 tgtctgctgc tgtttttgtg aaaaattttg ctcaaatttc atttttttgt ccaatcccct
     8761 tgtaattttt tatctgttta gttacaatta tcccagtgct tgcgtcctta gtgtagtgga
     8821 tatcacgtaa gattccggtt cttgagacgg gggttcgatt ccctcaggac gcgttctact
     8881 gctattttag cataatttac gaatagttgc ttctttattt ttaagtgaaa actgtccgcc
     8941 aa
//
LOCUS       DAZAFF010000106         8783 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000106 DAZAFF010000000
VERSION     DAZAFF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.108"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            220..1164
                     /gene="mvk"
                     /locus_tag="ACG9BB_002511"
     CDS             220..1164
                     /gene="mvk"
                     /locus_tag="ACG9BB_002511"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFN2838385.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            1157..2134
                     /gene="mvaD"
                     /locus_tag="ACG9BB_002512"
     CDS             1157..2134
                     /gene="mvaD"
                     /locus_tag="ACG9BB_002512"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFN2838386.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            2147..3232
                     /locus_tag="ACG9BB_002513"
     CDS             2147..3232
                     /locus_tag="ACG9BB_002513"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFN2838387.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            3229..4293
                     /gene="fni"
                     /locus_tag="ACG9BB_002514"
     CDS             3229..4293
                     /gene="fni"
                     /locus_tag="ACG9BB_002514"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFN2838388.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(4542..5345)
                     /locus_tag="ACG9BB_002515"
     CDS             complement(4542..5345)
                     /locus_tag="ACG9BB_002515"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFN2838389.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(5365..6207)
                     /gene="kduI"
                     /locus_tag="ACG9BB_002516"
     CDS             complement(5365..6207)
                     /gene="kduI"
                     /locus_tag="ACG9BB_002516"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFN2838390.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(6223..7230)
                     /locus_tag="ACG9BB_002517"
     CDS             complement(6223..7230)
                     /locus_tag="ACG9BB_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFN2838391.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(7237..7881)
                     /locus_tag="ACG9BB_002518"
     CDS             complement(7237..7881)
                     /locus_tag="ACG9BB_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFN2838392.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(7981..8739)
                     /locus_tag="ACG9BB_002519"
     CDS             complement(7981..8739)
                     /locus_tag="ACG9BB_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFN2838393.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
BASE COUNT         2506 a         1871 c         1741 g         2665 t
ORIGIN      
        1 agtcaaagaa gaaaaaaagt caaaaaattc ttaagaaaaa agtgtttttt ctttagaaaa
       61 cgtattctta gttttacttc tgagatggtt ttagtaaaat aaaggaggaa tgttttttga
      121 gacccttagt ggacaaaaaa attcgtgaag cagcgcaact cgttttgggc gctttatttt
      181 ttgcttaaaa atcactttta cgtgagaagg agttagatta tggcaaacta tggccaagga
      241 gaatcaagcg gaaagatcat attgatgggc gagcacgcgg ttgtttatgg agaaccagcg
      301 attgcctttc ctttctatgc aacaaaagtc accgcattcc ttgaagagct ggatgcaatg
      361 gacgatcaac tggtttcttc ctactattca ggaaatttag ccgaagctcc tcatgcatta
      421 aaaaatatca aaaaattatt cattcactta aaaaaacagc atgacatcca aaaaaacttg
      481 caactgacca ttgaaagcac gattcctgct gaacgtggaa tgggatcaag cgctgcagtc
      541 gccacagcag tcactcgtgc tttttatgat tacttagcat ttcctttgtc tcgtgaaata
      601 ctattagaaa atgtccagct ttcggaaaaa atcgcccatg gtaatcctag tggaatcgat
      661 gcagccgcta ctagcagctt gcagccgatt tattttacaa aagggcatcc tttcgactac
      721 ttttctttga acatcgatgc ttttttgatt gtcgctgata caggaatcaa aggacaaaca
      781 agagaagccg tcaaagatgt cgctcacctc tttgaaaccc agcctcatga aactggacaa
      841 atgatccaaa aattaggata cttgacgaag caagcaaaac aagcaatcat tgaaaattca
      901 ccagaaacgt tggcacagac aatggatgaa tcacaatcac ttctggaaaa gctgacaatc
      961 agcaatgatt ttcttaatct attgatccaa acagcaaaag ataccggggc attgggtgcc
     1021 aaattaactg gcggtggacg cggtggctgt atgattgcat tagcacaaac aaaaacaaaa
     1081 gcccaagaaa tcagtcaagc acttgaagat gcgggtgctg ctgaaacttg gatacaagga
     1141 ttaggagtac atacctatgt ttaaaggcaa agcacgcgca tataccaaca ttgctctaat
     1201 caaatattgg ggtaagaaaa atgaagaact catccttcca atgaacaaca gcctttcgtt
     1261 aacattggat gcattttata cagagacaga agtcatcttt tctgatagct atatggtaga
     1321 tgaattttat ctagatggca ccttgcaaga cgaaaaagca acaaaaaaag tcagtcagtt
     1381 tcttgacctt tttcgtaaag aagccggcct ctcactaaaa gcttcagtaa tcagtcagaa
     1441 tttcgttcct accgcagctg gattagcctc ctctgccagc gggctagctg ctttagcagg
     1501 cgcttgcaat actgctctta agcttggatt agatgatctc tctctttcaa gatttgctcg
     1561 acgcgggtct ggttcagctt gccgaagtat tttcggtggt ttcgtcgaat gggaaaaagg
     1621 ccatgacgac ttaagttctt acgctaagcc agtcccttcc gattctttcg aagacgattt
     1681 agcaatggtt ttcgttttga tcaacgacca gaaaaaagaa gtgtccagca gaaatgggat
     1741 gcgtcggaca gtcgaaacat ccagttttta tcaaggctgg ttagattccg ttgaagggga
     1801 tctatatcaa ttgaaacaag caatcaaaac aaaagatttc caacttctcg gagaaacgat
     1861 ggaaagaaac ggactaaaaa tgcatggaac gactttagct gcccagcctc cattcactta
     1921 ctggtctcct gacagcttaa aagcgatgga tgcggtgcga caattgagaa agcaaggcat
     1981 tccatgctac tttacgatgg atgctggccc taatgtcaaa gttttagtgg aaaacagcca
     2041 tttatctgag gtacaagaaa catttaccaa attatttagc aaagaacaag taattactgc
     2101 acatgctggt ccaggaattg cgattattga ataaagggag atttttatga ttgaagtatc
     2161 tgcaccaggc aaactctata ttgccggaga atatgcagtt gttgaaacag gccatccagc
     2221 agttatcgct gcagtcgatc aattcgtgac agtcactgta gaatctgcac gaaaagtcgg
     2281 aagtatccaa tctgcacaat atagtgggat gcctgtacgt tggacaagac gcaacggaga
     2341 attagtttta gatattcggg aaaatccttt tcattatatc cttgctgcta ttcgcttgac
     2401 tgaaaagtat gcacaagaaa aaaacattct tttatccttt tatgatctga aagtaacaag
     2461 tgagttagac agttcaaatg gccggaaata tggattgggt tctagcggtg ccgtgaccgt
     2521 tgcaacggtc aaagccttga atgtttttta cgcgttgaat ttatctcagt tggagatttt
     2581 caagattgca gcactagcca atttagcagt tcaagataat ggttcctgcg gcgacatcgc
     2641 tgctagctgt tatggtggct ggatcgcttt ctcaaccttc gaccatcctt ggctccaaga
     2701 acaagaaact cagcattcta tcagtgagtt acttgccctg gattggccag gtctatccat
     2761 tgagccattg attgctcctg aagatttacg tttattgatt ggttggacgg gtagccctgc
     2821 ctctacttct gatttggtcg atcaagttca ccgttcgaga gaagataaaa tggtggctta
     2881 tcagcttttc ttaaaaaata gtacagaatg tgtcaacgaa atgatcaaag ggtttaaaga
     2941 aaataatgta acgttgatcc aacagatgat tcgaaaaaac cgacaattac tgcatgattt
     3001 atctgcaatc actggggtcg tcatcgaaac gcctgctttg aacaaattgt gtaatttagc
     3061 tgaacagtat gaaggagccg caaaatcttc tggtgcaggt gggggcgatt gcggaatcgt
     3121 aattgttgac cagaaatctg gcattcttcc tttaatgagt gcatgggaaa aagcagaaat
     3181 cactccactg ccgttacatg tctatagcga tcaaagaaag gaaaaccgat gaatcgaaaa
     3241 gacgaacatg tttcactagc taaagccttt catgataaac aaaaaaacga atttgacttc
     3301 gtacggatca tccataaccc tttgccgcaa atagcagttt ctgatgtcga tctaagtaca
     3361 caggcagttg ggtttacact aagcagccca ttttatatca atgcgatgac tggcggaagc
     3421 gaaaaaacaa aaaaaatcaa ccaagatctt gcaatcgtcg caagagaagc agatttgatg
     3481 attgccactg gttcagtcag cgctgccttg aaagtccctt ctttagccga tacttatacg
     3541 attatgcgac aagaatatcc tcacgggaaa atcattgcca acatcggtgc agggacttct
     3601 gtcgaaagag ccaaagaagc tatccgattg tttcacgcag atgccttaca gattcatttg
     3661 aatgcacctc aagagttggt tatgcctgag ggagaccgtg attttaccaa ttggaaagta
     3721 cttattcaag aaactcaaac agccattgac gtccccctca tcgtaaaaga agtcggtttt
     3781 ggcatgacaa gagaaacgct caatgatcta gctgctttag gagttcatac agttgatatc
     3841 agcggtcgaa gcggaacaag cttcacccag atcgaaaacg cccgtcgctc caagcgggaa
     3901 ctgagttatt tagctgactg ggggcaatcg acagtctctt cattgcttga agcaaatgaa
     3961 gcagacactt ctatggaaat cctagcctcc ggaggtatcc gcaatgccta tgacatattt
     4021 aaagcccttt gtctcggcgc aaatgccgtg ggtacttcag gaaccgtact gacgcatctg
     4081 atgaatcacg gcgtagaaga aactattata ctgatgaagc aatggcaaga agagcttcgt
     4141 ctgctttata ctatggtcgg cgcaacgaat accgcggccc tacaccaaca atctctgatc
     4201 ttttccggac cagtgaagga ttggtgcgaa gcaagaggga tcgacttagt gaagtatgga
     4261 agacgcacag aaaagagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac
     4321 acgtacaaac ggcggaagta aaagcaactc cttcggaaat aagctgaaat tcacaaaaat
     4381 ttgaaaagca attttcgtga attccttctt atttcttgga gttaaatgct tctgtcccag
     4441 cctctttttt cgaataaatg atgtttaatc agctgtcctt cggtattcgt tcaatccgct
     4501 gatttcacaa cctctccact aaacagtgta ctaaagctct tttaaggttg ttttccgata
     4561 taggcaagta tgcctccgtc cacatacaag acatggccgt taacgaaatc agatgcgcta
     4621 gaagccaaga agacagatgg tccagccaaa tcgattggct cgccccagcg tgctgctggc
     4681 gtacgtccaa cgataaattg atcgaatgga tgacgttcac cgttttcttg tgtttcgcgt
     4741 aatggagcag tttgcggtgt agcaatataa cctggtccaa ttccattaca ttggatattg
     4801 tattgaccgt attcagaagc gatgtttttc gtcagcattt ttaatccgcc ttttgccgca
     4861 gcataagcac tgaccgtttc gcgtcctaat tcactcatca tggagcagat attgatgatt
     4921 ttaccgccgc ctttttcgat catatccgga ataactgctt ttgaaacgat gaatggggca
     4981 tttaaatcca cgtcgattac ttggcggaag tctgccgcat ccatttcgat catcggtgtc
     5041 cgtttgatga tccctgcatt gttgactaga atatcaactg aacctacatc ttctttgatc
     5101 tgttgaacca tcgcttgaac cgcttcttca tcggtcacat cgcagacata accttttgca
     5161 tcgatgcctg cttctttata atgcgctata ccttcatcaa ctgattcttg attcaagtta
     5221 ttaaatacga tttttgctcc tgctgtagct aaagagcgtg caatctcaaa tccaatacca
     5281 tagacagcac cggtgactaa tgctactttt ccatctaaac gaaacatatc catactaaat
     5341 tccatcattt tctctccctt atgcttattt taattgatcc atttgcacca tatccatgtc
     5401 tgtataggtg atattttctc cgcacattgc ccagatgaat gagtaattgc ttgtacctac
     5461 acctgaatgg atcgaccagc ttggcgaaat aatggcttgt tcattgtcta caactaaatg
     5521 ttttgtttca tctggtttgc ccatcatgtg gaacacacgt gtatcttcat tggcataatc
     5581 aaagtagaca taagcttcca tacgacgttc atgtgtatga caaggcatcg tattccaagc
     5641 gcttcctggt tctaaaatcg tataacccat ttgtaattga cagctttcgc atacatttgg
     5701 atggatgtat tggtagattt tacgttcatt cagtgtcagg ccttcaccag tctccattgg
     5761 ttttacttgg tcgatactga tttttacatt tggatattta tgatgagctg gaacagaact
     5821 aatatagaat ttagctgggt tttcagcatc ttgagaagaa aatacaacat gtcgtgtttc
     5881 ttttccgata tagtatccgt cttgtttttt catttcttct ttttgcccat cgatctcgat
     5941 gaatcctggt ccaccgatat tgatcacacc tagttcacga cgctctaaga agtaatcaac
     6001 acctagttct ttgtctaatg tgatttctaa ttctttttca gtaggtgtca ctccaccaaa
     6061 gatcattcga tcgttgtgcg tataagtcaa actgattttc cctggttcaa ataccttttc
     6121 tactaaaaat tcttttctca attgttccgt tgaataatga cggatatctt ctggactgtg
     6181 tgtataacgt gtttccattt cttgcatttt cttctactcc ttctatcgaa tgatttttcc
     6241 agagccgcat gctaaaaatt gtgcgacttc ttcttgacta aattggttgc agtccccatg
     6301 gatcgtatgc ttcaaggcag aagctgccgt agcaaaatca atgatttctt gcggagacat
     6361 attggataaa atcccatgga gcacgccacc tgcaaaagca tctccgccac ctactcgatc
     6421 cacgattggg gtgattctat ggacttggga ttccacatat tcatggttca tccacaatgt
     6481 ccccttcaat tcattttcac ttgctgaatg aacggtacgc ttggtcgaat aaaacacctg
     6541 aatattcggg aacttttcct gcattttttg ataataataa attagaggtt gttcttctgc
     6601 ttctggcgct ttctcgatac ctaacaaata aagagcatcc atttttccag ctgagcagat
     6661 atctactaaa ggtaaaagct gatggattac ttcgctggct tcttgctgtg tccataattt
     6721 tccacgatag ttgatatcga agctgatcag gcagccagct ttttttgcag cttctatcac
     6781 ctgtttcgtc attttttgcc attttttaga caaagctggg gtgattcctg aaacatgaaa
     6841 tatatctatt ccttggaata actcctctag cgaccattcc aaatgatcca tttcagcaaa
     6901 acttgatccg gcacgatcat agatcactga ggcggcgcgt tcgccactac cagtttccat
     6961 ataataagta cctagtcttg atccaccaga taatacctgt ttcgtatcta ctccataaca
     7021 ttgcaagtgc tttttgactg cttttcccaa tgcgttatca ggtacttttg tagcaaaaat
     7081 cgcttcatgt ccgtaattag ctaaagaaat tgctacgttt gcttcccctc cgccataatg
     7141 ggcagcgaat cgttcacttg aatcgatacg ggttccctgc tctgtggaca atcgcaacat
     7201 gatttctccg agtgtcacta cttttcccat aagcagctac ctcttgattt ctttcatttt
     7261 tgctgcatac tgttgcgcga cttccgttac tttgtcaaaa tctcctgtag ctgcgggtgc
     7321 taaaaggttc ccacctactc cgactgttac gacaccagca tcgaaccact ctttcatatt
     7381 gtccagactc acaccgccag ttggcatgat attgacttga ggcatcggcg ccttgaaggc
     7441 agaaatgata cttggtccat aagcgcttcc aggaaatagt ttgacgatat ctaccccgct
     7501 tttcagcgct gttttgatct cggtaatagt catacatcct ggtaagtaag gaatttgata
     7561 cagattgcac atctctgccg tttcttgatc aaaggaaggg ctaacaatat actctgcgcc
     7621 agctaagata gccaagcgag ctgtgacagc gtctaatact gtgcctgcgc caatcactat
     7681 ttctggttgg tctttgtaga atgacaaaag ttctttgatt acttgatctg cttgagggac
     7741 tgtaaaagtc aactcaatac cagttagacc acctttgatg attgcgtggc aagcattcaa
     7801 agcttcttct ttgctttttc cgcgtaccac cgcaatcact ccggcgtttt tcaatcgaga
     7861 taaaatatca atttttttca tcttctccac ctatttccta tttagaaatt ttattttata
     7921 acttggaaat ctatacccat attatacggc agaaaagaaa aattgcaaac gcttttttca
     7981 ttatttttga attctttgct cgatttcgct ttttgtttgc ttgagggcac tgattatttg
     8041 ttcttttgtc tcatctgtta aacgatattt cggcatactt acactaaatg caccaatgat
     8101 ttgttcgttt ttcacgattg aggctcctat acaaaaaata tcacgttcga tttcttcatc
     8161 gtcaaatgcg atgtgggttt cttttaccgt tttgatttcc tttttcaacc gtaatgcatt
     8221 ggtgatcgta tattctgtgt aagggatcaa cggatgcttt tcaagataat cagtgtactg
     8281 ctcttcactg aattctgcta atacggcttt gcccatcgca gaattgtata atggtcgggt
     8341 gattccgatt tttgaagaca tgcggatcgt ttgatgtttt ggatctagtt tgttcacgta
     8401 caagatctca ttgttgtcca atacccctaa gtggatagtc tcatcgatct tttgctgcag
     8461 ctgttctaaa aacggtaaag tcaactctac caaatcgatt tgttcaatgt ttttattcgc
     8521 gtagcgaata agctttgcac ctaaacgata gtttttctct gaatttttat ttacgtagcc
     8581 gatcatcaac aaagtatcta gtattttcaa gactgtagaa gaggtcattc ccacaccctt
     8641 tgcgatcgtc tgcaaagaga catcttggtt ttccgctaaa tagtctagta ttttagaggc
     8701 tttgatcaat acagttccat aaggcttttt attttccata taatattctc ctatttcttt
     8761 ataagaaaaa ttatagcgga aat
//
LOCUS       DAZAFF010000107         8776 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000107 DAZAFF010000000
VERSION     DAZAFF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.109"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(374..2755)
                     /locus_tag="ACG9BB_002520"
     CDS             complement(374..2755)
                     /locus_tag="ACG9BB_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFN2838394.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2745..3392)
                     /gene="recU"
                     /locus_tag="ACG9BB_002521"
     CDS             complement(2745..3392)
                     /gene="recU"
                     /locus_tag="ACG9BB_002521"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFN2838395.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3456..3995
                     /locus_tag="ACG9BB_002522"
     CDS             3456..3995
                     /locus_tag="ACG9BB_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFN2838396.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4085..4504
                     /gene="gpsB"
                     /locus_tag="ACG9BB_002523"
     CDS             4085..4504
                     /gene="gpsB"
                     /locus_tag="ACG9BB_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFN2838397.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4526..4893
                     /gene="rnpB"
                     /locus_tag="ACG9BB_002524"
     ncRNA           4526..4893
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG9BB_002524"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5102..6268
                     /locus_tag="ACG9BB_002525"
     CDS             5102..6268
                     /locus_tag="ACG9BB_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFN2838398.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6314..7810
                     /locus_tag="ACG9BB_002526"
     CDS             6314..7810
                     /locus_tag="ACG9BB_002526"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFN2838399.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7891..8574
                     /locus_tag="ACG9BB_002527"
     CDS             7891..8574
                     /locus_tag="ACG9BB_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN2838400.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8583..>8776
                     /locus_tag="ACG9BB_002528"
     CDS             8583..>8776
                     /locus_tag="ACG9BB_002528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838401.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2773 a         1585 c         1851 g         2567 t
ORIGIN      
        1 agacaaatta tgataattaa gcatactatc agataactca aaatgaagcc ttctttcaaa
       61 aaattattca aaaccatata ttgtattttt tatttgataa taacaatggg attattaaga
      121 agtggcacta aataaaagac tagtgtgtat ttagccgatt taattataaa caagtaaatg
      181 gataaagtat atgtaatgat aaaagaaaaa tggattttct aatttttaag ctaaatgtta
      241 gatcacacat gtatatgctg gatagtctta taaaaaatca tgtaatacag cttggtacat
      301 gatttttatt tgtagctgtt ttttacatag acacaaaaaa gaaaccacgc ttgtagacgt
      361 ggtttctttt ttactatctg tttccagaag ctgaggtgct gctggatggt gtatggtcat
      421 ttgcagcatc cgctccgcta gttgctggct cactggaaga agcaggagga gcagatgaag
      481 acggtgtcga gctttcttcg gatgaagaag aaggaaccgt tgttgaagac gctggctcgc
      541 ttgtttctgc tgtcgtgctt tcttttgagg actcagcagt tgattcgctt tgactagtag
      601 agctagagct ttgagtcgtt gtctctgttg ttgagcttcc aggagtctgg acatagctgg
      661 aagatggaat agttgttgat ggtgtaattg cattcgatct agctgtatat tgatctttgt
      721 agtacagttc accgcccaca cgaattaatc cgcttggcat ctcccagtct gtatttgtca
      781 cgttagctga aacatactgc ataagctcgc gataaacatc agaggctacg tgtgacgatt
      841 ctgaagtgac aggcgtcatt tttttattgt aacctgtcca cacggaaatc gaataatttg
      901 gcgtatagcc cgcaaatagg atatctggat acaccccaga agaaatacca agttttgcat
      961 actcgtcatc tgtgtaattg gatgttccgg tcttaccggc ttgatataac ccagcgattt
     1021 gcgcattcgt accagttcct tcagtgatcg tatcttttaa aatgtctgtg atcatataag
     1081 ctgtttctgg agacatggct gtttttccat ctggctcata agtttcttct gttccgtctt
     1141 ggaagacgat tttattaaca tattgaggtt tgtaataagt ccctccattg gcgaaagccg
     1201 cataggctgc tgccattttt aaggaagaag ctccgtattt tgtcccatct tgttcttccg
     1261 tattactaga gatcgcattc gattgatgga tcgtactgta ttcgatccca agatttttta
     1321 agaaagaagc cactttatct gaaccgactt cattgaataa tttgactgct ggaacattac
     1381 gggataagta aagtgcttgg cgtaaagtaa tggtgcccat atattgatta tcccagtttc
     1441 ctacaggagt cgaagtccct tcatagttat acggtgcgtc agtaatagtt tttccagtag
     1501 aatatttcaa atattcaaaa gctggtccgt aatctgtgac aggtttcatc gtagaaccaa
     1561 aatcacggga agtattcact gctaagttgt tgcctaaagt tacgtcttct gcgatgtgac
     1621 gtccgccaat ctgggctttt actttcccgg tattggtgtc gataagggtg gaggcaactt
     1681 gcatctcgtc gtctgggtaa gagacatact gatccgtgtt gacgatatca tatagtttct
     1741 tttgggcgtc cagatccaaa ttcgtataaa tctctaaacc gtctgtgtag acattcttat
     1801 ctgttttttc ttgtacttca ttgatgactt ctttgacata attatcgacg atcttagtat
     1861 tgtcatcgga ttgagttagt tcttgcagac catccgttac cggtacatta acagcttgat
     1921 catattctgt ttgagagatt ttttcgtttt gaagcatcgt atacaaaaca gtatctcgtc
     1981 ttttcttcgc ctgatctgga taaacataag gatcataggc ggaaggggct tgaggcatcc
     2041 cagcaagcaa tgctgtttgc ggtaaagaaa gttctgataa tttcttacca aagtacatct
     2101 cagaagctgt ctccatcccg tacaagccgt ttgacatata cactttattt acgtagtagg
     2161 tcaagatctc ttgtttcgat tttttttgtt ccaatcttac agccatccac gcttcttggg
     2221 ccttgcgttt cagagtctga tcctcggcac tggtggagaa gaaggaaagt ttgatcaatt
     2281 gctgtgtcaa agtacttccg ccttgcagtc cgccagaggt aaagttagag agggcagaac
     2341 cgatgatccg aatagggtca accccgatgt gtttataaaa acggcgatct tctaccgaca
     2401 cgatcgcatc ttctaatgtt ttcggtaatt cattcgcaga aattttttca cgcttttctg
     2461 cgcctaaatc ttcgaatagt tcgccatctt gtgtatatag tttggaagat acggtagcgt
     2521 ctaatttttt atccgtcaat tcgggcgcat cttttgcgta ataccagaaa agtcctacac
     2581 ctgctaaaaa gaggatacag aaaaatgaga gtattcctaa aaggatcttg ataaataatc
     2641 cttttttatg tgttccagag gactttccag aacctttgcc agaattcttc ttcacactct
     2701 ttttaggagt aggctgtttc tgtcgtctag agcttctagt ttgttcattt gccattcttg
     2761 ctttctcctt caatagataa tgttgataaa tagaagtcaa ccgcgtccaa ataaggaatc
     2821 cgcggagaaa taccagtttt gatctctatt cctagttctt cgatcaaaga taaaggtagt
     2881 gatttgcgtc ctttttcctt ttgggcgtcc cagtaagcaa tcaaatgaac actattcaaa
     2941 taaaaacagc gcttcaaaga cgaaaaccat aacaagacaa agcagacccc attttgttcg
     3001 aggcatgctt tcatatgttc gatttgatgg gcatggaagt ttttaaaggg aaaagatgtt
     3061 ttgttcttgg tttcttttgc ttcaaagtcc aagtaaaagc ctcgatagac gccattatag
     3121 tctgtagttg aggcttgggt aaagtaggct tccttgatca cagcagcact tctcttagga
     3181 tagtctactt tcacgatttg gataggggta ggctttttat ggataaccgc caacccgcgg
     3241 gaaagataat atttattact ttcattgatt gcttcttcaa agcgcatccc ccgattgccg
     3301 aattcagtga tctgatttct tttttcttga attttttcga cgggagaggc attcttataa
     3361 taaggttggc catttggata gcgcaatacc atctttaatc acactcctag gccacattat
     3421 agcaaaaaag cggatagaaa gaaaaggaat ctgagatgga taagtcaaaa gtaatctatg
     3481 tgtctgggta tcgaagtttc gaactcggtg tttttaaaga aaatgatcct aaaatccaag
     3541 ttgttaaaaa tgttttaaaa aaagaactcc atcagttagc agaagaaggg ctggaatggg
     3601 tacttgtcag tgggaatttg ggcgtcgaac tatgggctgt gcaaatagta gcggaattga
     3661 aagaagaata tccggaacta tctcttggta ttttgtatcc tttttctgat tttggtaata
     3721 actggaatga agggaaccaa gaaaaaaaac aattagcaga acaattagca gattatgtcg
     3781 aagctgttag ccatcaaccc taccaatcac ctagtcagct aaagaatcac actcgtttta
     3841 tacttgagca tacagtgggc tgtctgctga tctatgatga agaattccct ggaaaaacga
     3901 agtatttttt tgaagatgca cagaattacc aagcagatca tccctacgaa attcggctga
     3961 tcagtatgga cgatcttcaa aattttagtg aataatcgat attgtgtttg atttatcaac
     4021 ttttttccta tacaatgaaa aaggtagaaa tgttattacg taaatgaaaa ctagaggtgt
     4081 aaatatggcg aatttggttt atagtcctaa agacatttta caacaagaat tcaaaacaaa
     4141 aatgcgcggc tatgatcccg ttgaagttga tgaattctta gacaatatca tcaaagatta
     4201 tgaaacttac agcaaagaac tccttgcttt acaagaagaa aacgatcgat tgagtgcaaa
     4261 agtagctcaa ttatctaaaa ctcaaggagc agctcaaaca cgcgtgcaac aaacagaggc
     4321 accaaagagc gcagcagtga ccaattttga tatcttaaaa cgcttgtcaa atcttgaacg
     4381 tgaagtattt ggtaaaaagc ttgatcaaca agcaagtgca gtcaaaccag cacaacctaa
     4441 tccaaataac tacacaaatg cagacacaag cttagatgat aatgaaaaaa cacgtcaatt
     4501 ttaatttttg attaatgaat ttgcggtttt cgggtaatcg cggtctgact tgatcaggct
     4561 gaggaaagtc catgctcgca caggctgtga tgcctgtagt gttcgtgctt agcgaaacca
     4621 taagctaagg tactctttgg agtaacggca ggaaaacaag ctaaggcttc ggctatgctt
     4681 aagtatcctt gaaagtgcca cagtgacgaa gcggctgggg aaacccatcc ggtggaacgc
     4741 ggtaaacccc tcgagcgagc aacccaaaca atggtagggg cgctttttgt ctggaagaag
     4801 aacaggacaa aaaggcagta taactgcaga tagatggtta ccgctttgtt ttttctccct
     4861 ggaaaagcaa agtacagaac atggcttata gaaaaccgca aacatcaggg aattaggtcg
     4921 tgacaaaagt aatgcgttat aagaaacaag aaaaacattt gaaagctatt tttcaatact
     4981 tcagatgaat cgttcttatt acaaaacaaa cttttgtatc accggttatt cggaatttga
     5041 gtaaaagtgt ggggtaggac tgatgtccca cctccttttt ttataaataa ggagattgtg
     5101 tatggaaaaa aacaggaagt tcaacctcgt cgcaactgcg gcaagcgggc tagaagcact
     5161 ggttggtaaa gaattaagag atcttggaat cgaatgccaa gttgagaacg gacgtgcacg
     5221 ttttgaagga acaatggaaa cgatcgctac agctaattta tggttacgta cagcagatcg
     5281 agtaaaaatc gtggttgggg aattcgatgc gtatacgttt gatgaattgt ttgaaaacgt
     5341 aaaagcattg ccttgggaag attttcttcc actagatgca gcttttcctg tagctggaaa
     5401 atcgatcaag tcgaaactat atagcacacc tgattgccaa gcgatcacaa aaaaagcaat
     5461 cgttgaacgt ctccgtacct attatcaccg tccagcttct gtaccactga cggaaactgg
     5521 cgcacatttt cagttggaag tagctttgct gaaagaccat gtgatggtaa ctctggatac
     5581 gacaggacca agtttattca aacgtggtta tcgcttagaa aaaggtggag caccattgaa
     5641 agaaaacatg gcagcagcac ttattatgtt gacaaattgg cgaaaagacc gtccatttta
     5701 tgatcctgta tgtggttcag gtacgctttg tattgaagca gccttaatag gccataatat
     5761 cgcaccagga ttcaatcgtg agtttgcttg cgaatcttgg gattggttca gtcaagaaat
     5821 catggaaaat gttcgatcag cagccgaaga aaaagcagat tatgatattg aattagatat
     5881 tactggttca gatatcaatg gacggatgat tgaaattgcc aaagccaatg cagaagaaat
     5941 cggattaggc tcttcgatta cctttaagca acaagcagtc aaagacttca aaacggataa
     6001 agaatacggg gtcatcgtgg ctaatccgcc ttacggagaa cgtctaggag aagaagaaac
     6061 tgttcggaaa ttatataaag aaatgggcga tgtattccgt ccattaaaaa catggagtaa
     6121 atatatcttg actagcgact tagcgtttga ggaattttac ggacaaaaag caaccaaaaa
     6181 aagaaaattg tacaatggtg cattgcgtac ggatctattc cagtattggg gaactcgtcc
     6241 gcctagaaaa aaagagaaag acgaataatc ttactaattg attcattcaa ttggatgttt
     6301 ggaggaatag ggaatgaaag aaaaagagtt tttacaagaa gtaaaagaaa tcaatcttct
     6361 acaacaagct ttaggaatcc tcgattggga tacacaaaca ggtatgcctg aaaaagctag
     6421 tgcataccga agtgaagtag acagctatct ttacagcatt tatttttcaa aaaaaatcgg
     6481 tccaaaaatc aaagaagcca ttcgctattt ttctgaacat ccggaagaat tgtcggaagt
     6541 tggaactgcc gtttttgcca aagtaaaaga agagtatgat ttggaacatc gcgtaccgga
     6601 agatcttatg agaaaatata cagctgccgt ttcttcagca cacggacaat ggcaaaaagc
     6661 tagagaaaca caacaatttt ctgattttca acacgcattg aatcaaaata ttgaattaac
     6721 aaaacagttg ataccttact ggagaaaaga tgaaaagacg aattatgatg ttttgctgaa
     6781 tcaatacgaa cccgggatga ctgtcgaaat ccttgataag gtatttgatc agcttaaaga
     6841 aggaattctt tcgatccgcc gtacgctcca agaaaaaggg actgaacctg aaacagactt
     6901 tttaaatcgg agaatgacga aaaaacaaca gaagagattt gtagtgaaag taatcgaaca
     6961 acttggttat gattttagtc gtggtcgttt agatgacact gtccacccat ttatgactgg
     7021 aattaatccg aatgatgcac gaatcacgac acgatggaac gaaacagatt tcaaaatggc
     7081 tgtttttgga gtgatccacg aggctggtca tggtatgtat gaacaagaca tcgatgataa
     7141 atatgcgtat acaccggttt atgaaggtac atcaatgggt atccatgaat cacaatcgtt
     7201 gtttaacgag atcattattg gaagtagtcg cagcttctgg aaaaagcaat atcctttctt
     7261 tcaagaatgt gcagaaggaa cattcgatga tattcctttt gacaaatttt atcgttcatt
     7321 gaaccataca aaatcaagtt tggttcgtat cgaggcagac agtttgactt accctctcca
     7381 tatcatcatt cgttatgaaa tcgaaaaaat gattttcaat gaaggggtag atgttgctga
     7441 actacctgaa atatggaatc aaaaatatga agagtatctg ggaatacgac caacaaatga
     7501 tgtggaaggc atcctacaag acgttcattg gtcaggtgca agctttggtt attttccctc
     7561 ttatgcatta gggtacatgt atgcggcaca attacttcat tctttgcagc aagaaataga
     7621 tgtggaaaat gttctagcgt cagacgatta ttctccaatc aaaaactgga tgtcagagca
     7681 tatccatcaa tatggtgcat ctagaaaacc aaatcaatta atcatggatg caacacatga
     7741 accgcttaat ccgcaatacc tgatcgacta tatgaaagac ctgtatttta atgtatatca
     7801 ggtaaaataa aatacaattt acaaagatag ctgaaattca tctaatctat agatgttttc
     7861 agctatttac ttaagaaaga agtggaacga atggataaat tacaagtgat cgctgattat
     7921 agtatggacc aattagcgca tgatcaaaca ggacatggga gcgatcatac caaaagagtt
     7981 gtcaagttag ctgaaaaaat tttggagacg gaaccgcagg cagaccgctt tgtgacgctt
     8041 gcagcagctt acctacatga tacgattgat gataaagtcg ttaaaaatga gaacgaggca
     8101 aagcaacagc ttcgtgtatt ccttcgctct ttgccaatag tagaggaaca gatctcgatg
     8161 attttttcca ttatcgaaaa tatgtccttt tctaaaaatc tctctgaagc ggccgaacta
     8221 tctctggaag gtaagatcgt tcaagacgct gatcgtatcg aagcattagg agcaatcggc
     8281 attttgcgta ctgcttattt cggcggtgga catgggcacc cgatttttga tcctgaactg
     8341 tatccgcaaa cattcaagga caaagaaaat taccgaaaag gcacgacagt gatcaatcat
     8401 ttttatgaaa aactgttctt gctgtctgat aaaatgaata ctgattacgg atatgaagaa
     8461 gccaaacgaa gagaaacctt catgcgtgaa ttcttagaag aattttttct ggaatggtct
     8521 attgacgatg aaaattttac tccagacaat tggatcaaaa ataacaaaaa atgatggagg
     8581 ttgtgacaga agtggtcagc tccaagatat ttcggatcat tttccgtaaa agttacttct
     8641 ggaacactgt ttaccaagag gtcgtgagcc tgccgttcag ctccgagtca caaggaacaa
     8701 tttcaaaaaa tagcttctcg tgtttgatga atattgtaac ttgtgattcg gaattggcag
     8761 gagaacaccg tttatt
//
LOCUS       DAZAFF010000108         8361 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000108 DAZAFF010000000
VERSION     DAZAFF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.110"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..203
                     /locus_tag="ACG9BB_002529"
     CDS             <1..203
                     /locus_tag="ACG9BB_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFN2838402.1"
                     /translation="SDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVP
                     HILIDDSVSFANEEELIRLFEL"
     gene            complement(276..923)
                     /locus_tag="ACG9BB_002530"
     CDS             complement(276..923)
                     /locus_tag="ACG9BB_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2838403.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(931..1617)
                     /locus_tag="ACG9BB_002531"
     CDS             complement(931..1617)
                     /locus_tag="ACG9BB_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2838404.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(1623..2696)
                     /locus_tag="ACG9BB_002532"
     CDS             complement(1623..2696)
                     /locus_tag="ACG9BB_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2838405.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            2918..3592
                     /locus_tag="ACG9BB_002533"
     CDS             2918..3592
                     /locus_tag="ACG9BB_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN2838406.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            3597..4937
                     /locus_tag="ACG9BB_002534"
     CDS             3597..4937
                     /locus_tag="ACG9BB_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFN2838407.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(5086..6072)
                     /locus_tag="ACG9BB_002535"
     CDS             complement(5086..6072)
                     /locus_tag="ACG9BB_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFN2838408.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            6796..7827
                     /locus_tag="ACG9BB_002536"
     CDS             6796..7827
                     /locus_tag="ACG9BB_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2838409.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            7936..>8361
                     /locus_tag="ACG9BB_002537"
     CDS             7936..>8361
                     /locus_tag="ACG9BB_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFN2838410.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT         2654 a         1543 c         1432 g         2732 t
ORIGIN      
        1 tcagtgatat tcttgctgtg cataaaattc actctgagac aactgctatt actgaagatc
       61 agctgtcgaa atacaatctt ctcgagtatg atctgatcgt tacgaatcaa cccattttga
      121 atgcccatgt tcctcatata ttgattgatg atagtgtgtc attcgcaaat gaagaagaac
      181 tcatccgctt attcgagtta tgaataatca aaaaacagct atgtataaac caccctgata
      241 aaaatttcag gacaaataca catagctgtt tctttttatt gtattccttc agcaatccaa
      301 cgagatttat agcgaaagtc ttttaacaac tcttcttttg tccaattatt tgcaatgaga
      361 tccgtcaaag tgttcattac gagggtcatg gaaaagtata ggaaactaac tgcttcttcc
      421 gacgattgaa tggagaaatg attgattctt aataattcca gccatttttc gttcattgga
      481 gaatcagccg atagttctct acgtttggtt gtctgctgat catttcgctg ggttaacaag
      541 tataaccttt gccaatagat actttcgtga tctaagtcgc agcaccactc taaatatttt
      601 tcgatcccca aaaagaaatg ttgtttgttt tcgctaatga attttaagat atcttgatgg
      661 actatgtgag aactgctttt gatcgtatac aaataagcat cttccaaatc ttgaaaatat
      721 ttataaaaag caccgcgcga catccccatc ttgtcaacga tttctgagac tttgacttgg
      781 ctgatgtgct gtgagcaaaa tatctccagt aataggtcat cgattctctg tttctttgcg
      841 ttcgataaat tgaaaaaagt ttcttttggc atccacatct ctcctttatc tctgattata
      901 cgtggatagc ccttcattgt catgaaggat tcattccttc tttttctctt cttctgtcaa
      961 gtacccatct tccattcgat aaacgcgatc actccattga atcatacgga catcatgagt
     1021 aaccataata gtcgcttttt gtttctcatg agcttcttta actagtaatt tgacgacttc
     1081 atatgcatga tctgtatcca aactagctgt tggttcgtct gctaagatca agcttggctc
     1141 attatataaa gcgcgagcaa ttgccactct ctgtctttct ccaccagaaa ggtctttagg
     1201 ataactattt ttaagtgact ggatatctaa agattgcagc aaggaatcga tttttgttga
     1261 atcttctttt tttgaagtaa cttggtcaac tagttcaaat tgttccttta ccgttaaaaa
     1321 aggaatcaaa ttagagcttt ggagaataaa tcctatttct ttaaagcgta aatctgctcg
     1381 ttttttttct ggaagctcag ttagtgagtg accgttgatc actatttctc cggaagtcgg
     1441 tgtttgcaat cctcctgaaa tggtaaggaa agtactttta cctgaaccag aaggaccaat
     1501 gatgctgacg aattctcctt cctttacata aaaatcaatt gctttcaaag ctgagacctt
     1561 tgtatgacca gaaccaaatg ttttggtaac ttttttcatc gttaatattt ttttcatttt
     1621 tattaacctc ctattgctgt aattgggtct actttggtta tcgtacgtat cgaaaacaat
     1681 ccgcctagca ttgcgacaat gatcaaaatc ccactatata gcaaccattg tgaccaaaaa
     1741 acagcaaaag gcatcgcgct tggtaaaatc agactagtca aataagcaaa caaaagagca
     1801 agcagaacgc ctaccacccc aacaataaag gactgtgcta ctaaagattt agcgatgaag
     1861 aaatttttga tcccttgtgc tttcattact ccaaaaatag cagtcttctg aagtgtcatc
     1921 acgtacatga aaataccgac cacggcagca accactaaga acaaaaagta tatcatcgca
     1981 tttaaggtca tattttgggc agaatagcca ggaatacttt caatgaatgt cccaatagtt
     2041 aataactgta gacccttcga tgctttttct ttcgaaattt tagctgaatc ctttattacg
     2101 atcccattga tcggctgttc ttcttcagaa gcaaacggct gttgtccata cttcaaagca
     2161 gtccacgtat ctagggacgc atagatgacg ggagaaactg tgtaataagt ttcaggaaag
     2221 ataccgacaa tcgtcaaagg atcgtcgtaa ctgccaattt tgatcgtatc tccaatttta
     2281 tagcctgaat cagccagatt ttgagagatg atgatttcat tctttgtttt aaattcatgc
     2341 cctttcgtga gttttggtaa taaaaatgct tctttcgttg tgccgaaaac tgtaacattt
     2401 tctttgtttt ttcctttgat tgctccgctg tacaatccga ttggtgcttt ctctcctcct
     2461 tcaatgtatt tcagatctcc tcttgttaat tgtgaagctg caaatacttg atttgcatcc
     2521 tccgacaaaa caatctcttg tgcgtcccaa tcatcgattg cttttttaaa ctcttctgaa
     2581 agtccattcg ctagtcctga caacatgaat acgacatacg caatcaagaa cataataccg
     2641 acgatcaagc catatcgtaa tttagaatag cgaatttctt tgatagctag aaacatttgt
     2701 ttttcctccc tatgatagat tgatagtgac aaaatgtcac ttgttgaata gtgtaactct
     2761 aaagtgacaa tttgtcactt gtgttgtttt tttcttatca ataaattgtc taagacatct
     2821 ttcgataaaa ccttaacatg agtcataaaa aatttattta agccttattt atgctttcca
     2881 tgataaacta aagtgaccaa atcaatgagg tgggaaaatg attaaactat tagtagtaga
     2941 agacgataaa gtattatcca ataatataaa agaaatactc gctgcaatcg gcgagataac
     3001 acaagtttat gatggtagtg aaggactgtt cgagttggaa agtggagttt atgatcttgc
     3061 aatcttggat ttgatgatgc cggaaatgaa tggatatgaa gtgttagcaa aaatacggaa
     3121 agaaaaaata cagactccag tattgatttt gactgcaaaa gatagtttgg aagataagat
     3181 tgaaggattt gaacgaggtg cagatgatta tctaacaaaa cctttctatc gagaagaact
     3241 gatcatgcgc gtgaaagcat tattgaaacg ctcgctaggt ctctttgatg aaaatgttct
     3301 gaagtataag gatatcagtt gtgatttaaa aagcaatgaa gtcttgtata aagaacaagt
     3361 tttgcccata caaggaaaag agtttgaatt gctcgtttat tttttacaaa ataagggaat
     3421 cattttgaca aaagagcaga tatttgaccg gatctgggga tttgattctg atacgacgat
     3481 tacagttatt gaagtttata tgagcaactt acgaaaacat ctgcgaagta ctggattgga
     3541 taaagaaatc aaaaccttac gtaatgtcgg ttatatactg caaagggtgt gatgcaatga
     3601 atggagatat tacacgaaag caacgtagtc gcttttttgc gacaaatgta gcagcatttg
     3661 cttttatctt ttttcttttg ggattcatta ctttacagat attaaaccaa tctgcataca
     3721 gagaaacaga tctgtcatta gagaaaataa attttgattc tcgaacagta caattagaga
     3781 tacaaagata tcagcaaggc gatccctttt taaacccgac tcaaacggaa caaaacagaa
     3841 caaacatgga acagccatta atggaaccat caaatgaccg ttttaacact caagtgatct
     3901 tgtggtctaa aaatggtgag atattgaata aagcagctat tggtgggcgt ttcaaccaaa
     3961 tcaaagaatt aaaaatggat acgaaaaatc tgaataccat ccaaaatatt gaattaaatg
     4021 aagacagtgg ctcatctgcc ctctctttcc gttcgattac gaaaaaagcg gagacgaatc
     4081 aagatggcgt tgcctacatc caaattttag aaaatacaaa tcaaatcaag aattcattag
     4141 cgacttttcg cacgattatg attttgtgta tgattgtttt ttggattctc tctattggta
     4201 tcagttatta cttgtctagt ctaagcatgc gccctattat tctttcttgg agaagacaaa
     4261 aagaatttgt tgagaatgct tctcatgaat tgcgcacacc gttaacgatt attcaaaata
     4321 gtttggaaca tctttttacc aagcctaatc atactattat tgaagaatcc gaaagcatcg
     4381 ctcaagcatt gtcagagacg aggcgcctca ctggattgac ttcggatttg ctgacaatcg
     4441 ctcggaatga ttctgatcag cagctgcttt caaagcaaat gatcaataca caagaatata
     4501 tcgaacaact tgttaaacca tttcaagaga tggccatcat agatggaaaa gagtttattc
     4561 tccataacga tgcaacatta gaagtaccaa ttgatgaaaa aaaaatccat caagttttgg
     4621 ttattttgct ggataatgca ttaaaatata cgaatccaga agataagatc attttggatt
     4681 catctgttaa ccataaagaa tggctgattc acgtaaagaa tactggccct tctatcagtg
     4741 aagaagatca agcaaggatt tttgatcgat tttatcggga agatcaatct cgggcgaaag
     4801 agactggcgg atacgggctt ggcttggcaa ttgcaaaaca ggtaatcgaa cagcatcatg
     4861 gacagatttc tgtatccaat tatcttccac aaggagtcga ttttaaagtc caattgccgt
     4921 tgaagcctaa aaaataatgc ttatccctaa aaaagggctg tgacaaaaat cttgtcacag
     4981 cccttttttg ttgcgaataa acggtgttcc agaagtagct tcttcggaaa taagccgaaa
     5041 tttctttaag ctgaccactt ctgtcacaac ctctttagct tacgcttacg attttttatt
     5101 gcggtcttca atgtgtacat caccgcttgt gtcaatatcc aattcttctc ggctaacatc
     5161 ttctgtcact ttttcaacat cttcatgttc ttctttatga atcccaactt cttcacgaat
     5221 aactgtatgt ttgcttacat ctacttgctc ttctttgatt gggatagtaa tcgtgtcatc
     5281 ttccaatttt tcgtctgttc cctgtgaaga ttgatctgtt actggtttgc gttcgatgac
     5341 gatttcttca cgtttgaccg gtacttctac cgtttcagta tcgttgacca catgtttgtg
     5401 gatgtcgact tctccagttg tcacatcatg tgtatttaca tctaattttt cttcttttag
     5461 ttgaattgta tcatcatcta gatttggttt gcgatcgatg tcttcagtct tgcttgtacc
     5521 cattgtatca gtctcgccag gtacgtccat cggatcagtt cctggtgtca tattcaatgg
     5581 atccgttgta ttttgaaagc ctgtcgtgcc acctacgtcc attggatctg ttccagtgcc
     5641 agtttctaat ccagtcccca ttgtgcttgc tgggctaaca gtatcttgtg tatccattgt
     5701 atcttctgtt gtttctggct gacgatagcc ttttactgtg atgacaaatt ttccatcaga
     5761 aatatctcct ctatattgat ataaaggatc attttctggt gtagccgtct cagaatcata
     5821 ggtatcaaaa gaaaaagtat cttttatttt ttcccataaa gaacggtctt cttcaccatc
     5881 catccgattt tcatctgttt caacatctat accagacaat ccgtcaaatc ctagtctaga
     5941 tgctgcttca tcagtagtat aaataacgat atcatctcgt tcataccctt cgttacgcaa
     6001 ccgttcaatt actgataatg cttcttccct cgtactatag cttccgatga ccgttaaatt
     6061 tttctcttcc atgtaaattc ctcctagcaa atagatttat gattggtata ttgttactta
     6121 taaatttatt ctataggaga cgattgtttt ctacaaatat ttagattgga atttgattaa
     6181 aacaatttaa ttgaaatttg aaaacattta tgttaatgaa agtcatattc taataaaatg
     6241 tcatttgttt ctatatgaat ttttatgaaa aatttcatat aatttccact cgttgtgatg
     6301 gtttaattta tccaatccat caataagcaa acaacttata taaaaaagga ttaattctca
     6361 atataattgc tcctaccata gaataaatct tcatgtaaag taatgaagta ccaactattt
     6421 ctaatacttc tttaagaatt atcccaacaa tatcgttaga caaaccatta ttgaataaat
     6481 ggagagccaa tcgattctga aaaaatgttt tctaattttt cggaaataaa agaaagagcc
     6541 tcctgccatt agaacgaacc aaatccattt cattgctcat gaagtaacca catagctaaa
     6601 aagaggataa atcaattaaa agatcaaaag aatgactgca ctaataaaat aactaaaaat
     6661 gtatcttctt acttcatatc ttttagctag tacttagtta aaaatttgct tcctaagatc
     6721 aagacaaact gtctatcttc atttgatgaa atgcggtaaa atatagaaaa tgaacactat
     6781 tggagggaat ttgtaatgga cagcaaaact agaaaacaac tgcaagaaaa gttaaagcaa
     6841 cgtacacact tgctcccttc cgtctttcaa cagtttttag aagaaaatcg ttcttccctt
     6901 tcattaggca gtcgctatga atacgcaaaa gattttcatc tgtttactga ttatttgaga
     6961 aaagtatttt ctgaagaagt ggatgatcag ctgattatcc atttagaaaa acaagattat
     7021 cagaatttct tagctaaaat ctatcgacat actcgttcgt tccaaactac agcgaaccaa
     7081 gaaacggagc aattgtttga aaatagctat tacgggattt caaggaagca atattcatta
     7141 aatcattttt tacgattttt gtatcaaaaa ggactgattg aagaagagat ttctcttttg
     7201 ggtacaagtt taccggaaac aaccaaagca gctccgcgat atatcgatgc agaacttttc
     7261 aaagattttg agcatttgtt tatcccgatt gaaggctttc aaacaagcag agaagctagc
     7321 tttgaagaaa agaatagacc gaggaacctt gcaattactg ccattcttct gtattgcgga
     7381 ttaaagatcc atgaagtagt agaacttaaa cgtaaagatg tatctttgac caagcagatt
     7441 cttaatctga atagaaaaga aaacagactg aataaagtac caataccaaa agaagcgatg
     7501 ccatttctgg agaactattt taagttaaca gcacaaaata cggacgaaaa ttcttttgtt
     7561 tttcgttcct cacatgatca gcaaatttct cctcggacta ttcgccaaat tttgagcaaa
     7621 attacagtat ataaaaacgt atcgccagaa ctatgcagaa aaacatatcg ttattatgca
     7681 gaactatatt tagatgatgc tgacgcggta aattatttat gtggtaatcg atacctctca
     7741 tcccattctt ttcaagatat ctgggaaaag atggttgatt tttcttatca cgaattagcg
     7801 gcattcgttc gggtttcttc catataaaac aaatgtatga tctttttttc acaataaact
     7861 atgttattat ataaaggatt atgttacgat tatcttgaat ttgtatatta cttaacaaaa
     7921 gataatggag tgtgaatgat ggagaccata acgtattccc ctttgaactt atttacgaat
     7981 tttcaagaag ctgcagctaa cttttccgag accccgatta tctttgatga gccactttca
     8041 gctttcccag aattaggact tgaaacaact tacaaaaaat gctgcgaaag tgtgttgaaa
     8101 cgggcttatc aattggctca tcttggagtg aaatccggag acaaagttat tatttataag
     8161 agttccgctt ttgacacata ccttctagct gtttctgtgt cttaccttgg tgcagtgcct
     8221 gtcatgactt cttatcatct accaacaaca acaatggaag tatttatcga tcgtttagaa
     8281 gaccctttca ttttatttga tgatgagaca aaaaagcgag tgagagaaat ttcaaatgga
     8341 acaaaatcaa aacagattcc t
//
LOCUS       DAZAFF010000109         8037 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000109 DAZAFF010000000
VERSION     DAZAFF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.111"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..199)
                     /locus_tag="ACG9BB_002538"
     CDS             complement(<1..199)
                     /locus_tag="ACG9BB_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838411.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
     gene            complement(362..1996)
                     /locus_tag="ACG9BB_002539"
     CDS             complement(362..1996)
                     /locus_tag="ACG9BB_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2838412.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2000..3373)
                     /locus_tag="ACG9BB_002540"
     CDS             complement(2000..3373)
                     /locus_tag="ACG9BB_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFN2838413.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3389..3628)
                     /locus_tag="ACG9BB_002541"
     CDS             complement(3389..3628)
                     /locus_tag="ACG9BB_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838414.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3810..6485)
                     /locus_tag="ACG9BB_002542"
     CDS             complement(3810..6485)
                     /locus_tag="ACG9BB_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFN2838415.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6668..7591)
                     /locus_tag="ACG9BB_002543"
     CDS             complement(6668..7591)
                     /locus_tag="ACG9BB_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838416.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT         2361 a         1580 c         1207 g         2889 t
ORIGIN      
        1 tataataaac atttccttga ggtttccttt taatcccctt cggaaaatca ccttttgtaa
       61 aatcgctctt aaggaaatca tctaaattac cagttctggt ctttttacct ccagtattcc
      121 ctgatttttt aggtggtaca tgttctttag taagttcagt gtatttttca cataaaatac
      181 acttacccat ctttttcata tctatcccca ctctttttaa ttcttatata caattatatt
      241 tcaaatattc attaataaat atccctattt atgataagga cctccctaac tacttctcta
      301 acgatttcaa atcgctagag aagcgttagc gcccctctaa cgttgtttga tataacctag
      361 actagatcgt taacgtaaag ggtgttttta acacaaaaat agccgtatcc tttgttttgg
      421 atacggctat ttgatattgc tcattcaatt gttgtaccac ggcatgcgtg aatttcatcg
      481 taatttccaa catctcctta tggttaggat caaaggttat tttctctata aaggtacgat
      541 acaaggcttt gatttgcttc ttctcactct gggcaagaaa acgatccaaa ctagtcaata
      601 tccgttggac atctttgact tgaatcggct ctccttgttg aaagatccct aagatttcat
      661 tttctcgaat gtgcaactgt cgtcgttttt cagtcaattc gtctaagcgg tcttttaaca
      721 tcggaaacaa ttcaggacta tcttccaaag cagcttccca tttttcaatt ttagtcttca
      781 attcttcctt tcgttccagt aacaccacga gttcttgttc caatggttgg ctttgtcgca
      841 ctatttcttc atttaacgct gccaccaatc ggggaagaac ttctggtagc tgaacgatct
      901 cctttaatcg gtcggcgaca aatgtttccg cttgttcggc acgaatcgaa ttggcatgac
      961 acacacttgc tcctttattc cgaaagactg aacaagaata atatcgaatc cgtttctttg
     1021 tgccatcctt tagggtattt gtgacattac tcgctgccat cggtgcgcca cattctggac
     1081 aacgtaacaa gcctgtcaat acattttcac ctgtatggtt ccatttgggt tgttttcctt
     1141 ctaaagcaag gcgttcttgt actttttgat aatcttcttc cgaaataatc ggttcatggg
     1201 ttcctttcac taaaatcggt ctcggattct tccccttccg tcgtttggtt tcccaattga
     1261 tgtagcgcgc atattctaaa taccctgcat aaattttatt gcgtaagata tctttgactg
     1321 ccgtggtaga aaaagcattc tttttgactg tttgatatcc ttgacgattc aaaaaattgg
     1381 caatcgctcg ataccctgct ccgcctaaat attttgcata gatcagacga acaattttcg
     1441 cctcttctgg ttcaatgacc aattgttttt gaccattcgt gtcaatacca ctgacatagc
     1501 ctaaacgtcg gcctcctgca taacgctttt tatctttcac taagctttgc attgacattt
     1561 gaacattctc cgcaatttgg ttgcgttcaa attcaccgaa actcccaagg agttgtaaca
     1621 tcaatttacc tgtcgaagtg gtcacgtcaa attgttccga gatcgaatgt aatcctacgt
     1681 catatcgcaa taattcctcg actgtttgta agacatctaa catattgcgt ccgagacggg
     1741 tcaatttcca aatcactagt agatcaaaac gatggttccg agcatcgttc atcattcgtt
     1801 gataggcgaa ccgatttttt gtggatttcc cactgattcc ttcatcggca taaacttcta
     1861 ccacttcata gcccatccgt tcggcaaact gtcgtccatt ttgttcttgt gcttgaatac
     1921 tatgtccttc taaagcttgt tcttgtgtac tcacgcgaat atacaatgcg actcgtttgg
     1981 tttgtatcat ttccatagat cattcgttcc tttctaaaat attttgtgtc agttgtcgga
     2041 agtaaatccc acaaactgtt ttatcctttg ttacctcaat ccgacaaaca tagtcacata
     2101 ataattgtcg actagagagg tcttttagtt gaagccattc tttcactttt gcttcacact
     2161 gttggagtga aagaaacgaa gaagtagaca tcggcttttg aagagcttgc agttgttgct
     2221 tgagttcttc cagtgaacaa ttgccctctt caaatgcctt aatccattgt gcttcttgtt
     2281 tcttttgttg gattactttg cgtttttcat cttttcgctt ttcggctaat tgttgcgtta
     2341 attgggcaat caaggcttga tagacgcctt cgtattgaaa aaaagcctgt atcttttcca
     2401 atacttctga ctcaaccctt tctgcccgaa gaaaaattcc ttggcagctt cggtcggtac
     2461 aatgataata atgaagcgtc ttcgtcttct ttgtattcca ttgatacttc gaactcagat
     2521 gacgaccaca gaagggacac cgaatttttt gacgtaataa gtacagatac gtatcttttt
     2581 tagctgtaca ccttgattgg cgaatctctt gggcttgtaa aaaggttgct ttcgagagaa
     2641 tcggttcaaa ggttcctaga tacttcccca aagatcctcc tttgatttcg ccatagtaca
     2701 atggattttt taggatacta taaatatgat agggctgaaa aggacgttcg cggaagaaaa
     2761 gcttttgttg cgtcaatcgt tgacaaagct ttttgtagcc atattttttg gtcaaataaa
     2821 gttgaaaaat atagcgaaca atccttgctt cgtgtacttc ttgttggatt ttcccattca
     2881 ctaaacgata cccatatggt accgcagaag acaaatattg ccgttgttgc tttttatagg
     2941 aaagtccggc acgaacattc tcaataatga cgtttcgttg ttgttctgca aaagtgaaca
     3001 ataattgcac aaatagttta ttcgttgacg cgtgtgagaa agaagcagga agaggttcag
     3061 aaatcgaata cacttgaacc tgatgttgtt ggcacaactc aataaattct aaggctattt
     3121 tggcttgacg tgccaatcga tcataacgcc aaacatagat caccccctct tcttctgacg
     3181 cacaaaccca agctctaagc tgtcgaaaag ctggacgctc cgctttttcc cctgattctc
     3241 ctttgtctga aaatcgttgg ggaatttgtt catatttctc taaaacatac tctttcactg
     3301 catcatattg agcgtcatta ctaattttcg cttcttcata aacagacgtt cgatcataac
     3361 aagccacacg catgcttgat tcccctctct atgaatgaac taaataccat tttttcacga
     3421 ctcttctatt gaacgaacgt tgcacaaagt gattttctaa atatccttcg ttcttcaacg
     3481 cttgaagagt acccccgata gatccagcag atacccctct tatttcgaaa tccaagtaaa
     3541 ttggttttgt aaaaaaggct tgatttgaaa aatgttcttg aatatacaga agaacctctg
     3601 cttttagtgt cattttattt ttagtcatta gaattttcct ttctttcatg tttgattgaa
     3661 tgaagtaaat gaagcataga tcaagcttca tttacttccg tttgggtttg ctcgctatca
     3721 tacagataag taatcagttg ttttgctaaa tcaataagaa atatttccat aacatggtgc
     3781 ctcactttca tactttggtt tattctaaat tacaatcgaa aatacgattt gagtgaagaa
     3841 ggtaatagaa tctcgtatgt tggaagaact tgacgattca tgtgaatacg tttcgttctt
     3901 ctcccttttt ctgcacacat attacccgaa tcaagcaatt gcctaaaaag ataggttaga
     3961 tcctttactt gaaattctcg ccttaacaag tcctcaaacg ggataggaaa aatatttacc
     4021 ttaatatccc catttttttg aattgatact tttccccaaa tcgtactggg cgtatgtttg
     4081 ttgtcactgt ttgttaaaaa tttatggcta ttcatcacca acaatgtttt cactcggtca
     4141 aataccgttt gctcacttcc taaactagta tctacaatta actgttcgta cgaattaata
     4201 agcaccgtct gtatatgctc taatcgtaaa ggatagccca gtatatcttg aattaggagg
     4261 gctccactat aaatcaaagc tagattatta ccaatgcgtt gcttcaaaga atgttcaggt
     4321 aaaacagccc atagtttttt ttgagcttcg acaaaagttg cttcaatttt tgtaatccct
     4381 tgttcaaaga gtttcaaaag aaataacttt ataccccaac caaaagtatt agaagaaaga
     4441 cttttaactt tctctgcgtg attcgcatcc tttgtaaact tgatatcaga aaattcgata
     4501 tatcgaactt tcaaaccagt taatttctca ttgaaataat tttccaaacg ctcttcagaa
     4561 gaggtaaggg tcaaaacaga aaaagtctct tgtggcataa gctgacaatt agaatctaat
     4621 cttagccttt cttctcctga agctaattga tagatcaacg agctaaagtt attttgagtt
     4681 gctgctccta actcatctag tattagtgga acacctccct gattgtttga tgctagcttt
     4741 ataatggctt tattggtagc attcatattt ctaagaaccg tagttggatt tccaaataaa
     4801 gaggcaataa agtgtaacgt agtggtttta cccgttgtgc tcttgccctg taggttaatt
     4861 agtaaactca aaaaagtatt agccttcgta acgtttaaat agcctaacac aatagaagca
     4921 ataccaatcg accaagcaag ttctaagttc gttcctaaaa catacttatg aaccaatgcg
     4981 tcaaattccg cttctgtacc attagttgtt aattggtagc atgagttttt agttaataca
     5041 gaaggaattt cacgtccaaa agaatcttgt aactgaaaaa tcattgcttc attttctttt
     5101 ttccagccaa gagattcatg ttcaaaaaag attggagcat tctcccgttg aatctgtaaa
     5161 aagtcaacga cttcagcttc tcttctagct ttaatgatta agccatgttt tgctaattgt
     5221 ttaaaattat tagtttgtaa acatccacct tctatttcta tttcatccca acaatgatcg
     5281 acataatagg caagaacaag tgtaatgact ttttgttcga tatttttgcg tatttgctta
     5341 ataaacatcg gacaagcaac tttttcttct tgaccttctt tcaccaaaaa tactccttct
     5401 tgattcagtt taaactggtc aaaataatat tcttgattgg attctaatga ttctacagaa
     5461 ggatttacag gttttggcaa cggatatttt tttacaaagt cttgaatatc tttattttta
     5521 tccgaagcat gcagacaatg aaatcgaatc ttattatcag ccgtaacaga caaacaagaa
     5581 ccattagaat tattcgtgtg ttttcttaat ggacattcgt caaatataag taaggtaact
     5641 cctttatgat cgccctcttt tatggtataa gttaattcag gatagtgagc taaccattgt
     5701 ttatagtctg cttgaataaa aggtaacact tctctaaaag atggatggct ttttactcta
     5761 gaggttgaca tcatggaatt ttcttcaact atctgttcaa gaattttaaa acaattactt
     5821 ctatctatgt tatcgggaat agaaaggaaa ctagataaac gatgcggacg ctcttttgtg
     5881 gactttcctt tgaccgctgg tgtaccaact aatttgcata atcttgaagg attataaaca
     5941 gaagtatcaa tatcaacttc atcttctact ccaaatcttc ggtgaagtaa ttgaagaaaa
     6001 ttctgaatag taagaatcgt ctcgtgatta tttggttgtt catttatggg aagaaaaata
     6061 tggtagccat tcccactatc aacttttaca aatgattcta ttctcttttc tttcaaataa
     6121 cgttctactt gttccataac attttctgcc tttttctttt cgctatctgt tgcacttccc
     6181 tctttccttt tagggtcaat atcaataaat acaaaccgaa ttttttcaat atcattgtct
     6241 ctcacagaat gctttttttg tgtaatcacc tcttgtttaa taggattata agttatgttt
     6301 actgtacctt cagacaattt ctgtttttct aaatagttaa taattgcttg agaatctgca
     6361 gtagaaaaaa tatgagaaac ggttttcttg tttaatgtag aaaaagttct caaattaaca
     6421 taagaaatcc ccagtgtatt taacagttgt aaatgcttct gaattgaagt gtgattcatt
     6481 ttcattaatt accactcact ttctaaaaaa tttttatttg tatgactaca ttacctccaa
     6541 cctctcataa tttcatgatc ttcagttcta caaaaatata tcccactcaa aatcttgaca
     6601 aaagaaaaaa gttgtgattt tggaaatcac aacttttttc ttttacgata aatcaatcct
     6661 tattaaatta tttaggaaaa tattgttcaa ttatttcttt atatattttc tcatagtatg
     6721 tttctgtcca tcctaatgtt cctataaaat caagtcctct acattttaaa ggttcactta
     6781 aactattaat atagttaata atgttgtcta tccttttctc tttatcttca ccttcaatct
     6841 ggggagcata tagtccaatt tcctcccaat aatatgaatc tctttcaatt cccatgatgg
     6901 tagctttggc taatttcaaa tcaaataacg attttagaat tcttagtgtc tcaataaagt
     6961 cttctctctt caactcttta atccattgtg aattttctgg attatctaaa atttcaagtt
     7021 catcttcatt ataagtatat ttcaaatttg acttgcttaa ctcatagata aatgaagttt
     7081 cttgactcat aattctttta agtacttgac tataagtttc gtttgacttt gcccatttcg
     7141 ctaattctac cactgtattc actaataaat cgaaataatg atgttctcgt tcaatagtct
     7201 cagcattttg aaaatgaaaa tcacctttat aaactccatt cttttccact gttttattaa
     7261 tattttttaa aaaccagaat gagtttgcaa atttagtcgt actttttcga ttaactaagt
     7321 taataactct tccatgtaat aataagacaa aaatacataa atttttagct tccttattat
     7381 tagatactaa ttgatcaatc atagggagcc attttttgat tttcactaat tgattgctca
     7441 taaatctttc actggacttt tgttcaatct taaattgcaa aacttctaac tcataattcc
     7501 catcttctga tttatttttg ggaacaacta tcaaactttt ataaagttta tccacatcaa
     7561 aatttatctt ttttgcataa tttgtagcca ttaatacatc tccttattca taataaattt
     7621 catatgacgc tcctctcaaa atttaccggt agactagaaa aaagaataaa agatacgtca
     7681 attcacactt tattcttaaa aaaaaactgg tctctctacc ggtaatatcg attgagtccg
     7741 cctttatcga ctttatcctt tttttatatt aaattttttc accggtagac agggagagaa
     7801 agaataaact aaaaagccct taattcttaa tgttttcacc tccttaccgt tcctaccgct
     7861 aaaatttaat ataactaaat tctataacat tagaagaaat tttaaatacc tttgataaaa
     7921 aatattgatg ttttttctct cattgtttac aaactagata gcttatgtat tcttattaaa
     7981 aataatagat atttcgaaca tcatcactct catctactta atttcgcatt tttactt
//
LOCUS       DAZAFF010000110         7998 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000110 DAZAFF010000000
VERSION     DAZAFF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.112"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            387..575
                     /locus_tag="ACG9BB_002544"
     CDS             387..575
                     /locus_tag="ACG9BB_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFN2838417.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            653..1666
                     /locus_tag="ACG9BB_002545"
     CDS             653..1666
                     /locus_tag="ACG9BB_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN2838418.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1733..2767)
                     /locus_tag="ACG9BB_002546"
     CDS             complement(1733..2767)
                     /locus_tag="ACG9BB_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2838419.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2882..5107
                     /locus_tag="ACG9BB_002547"
     CDS             2882..5107
                     /locus_tag="ACG9BB_002547"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN2838420.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5125..7008
                     /locus_tag="ACG9BB_002548"
     CDS             5125..7008
                     /locus_tag="ACG9BB_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFN2838421.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6989..7963
                     /locus_tag="ACG9BB_002549"
     CDS             6989..7963
                     /locus_tag="ACG9BB_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFN2838422.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2578 a         1459 c         1695 g         2266 t
ORIGIN      
        1 ttttagttag tctacattat tttggcgatt tgccaataag gactgttgag ttgtgcaaaa
       61 tcaaatctgg gtcagaatga acgtcattct gtctcagatt tttcgttatt tcaatttgat
      121 taactgattg atcaaaaaga tttttatttt catattctca aatgattcaa cactaaatat
      181 acatgtttga cgcggaaatt attattcgat tttggcggca tcagggggga tgacgacgta
      241 gcagacccat tattttactc aagaaaatgt ccgtgcctat tatttagaat acgatagtga
      301 acgtgctggg agttttgttc ttttagagaa agtctctgaa gataaattag ttgtcttaga
      361 ttttgactgt aacgaaaaat ggcgaaatgg aagagcacca gcaagtgatt gaccggatct
      421 acgaagcgac acgatatctg cctttggatc gtttgtgctt aagtcctcaa tgtggatttg
      481 cttcaacaga agaagaaaat gttttgtttg aagaacaatg gaaaaaattt cttttgtcaa
      541 aagcattgca gaagaagttt ggggaaaatc attaaatcat ctaggagagc ttgataggaa
      601 ataacactat ggtataatca gaatgcttaa tactaactac tgagggagcc ttatgaaaaa
      661 gaaaattctt aaatttatcg gatggttctt cggaatcatt cttgggatcg cgtttgcaat
      721 ctttcttgcc ttccagcttt cccccagacc tggagcattc ttgatcaacc atatgttttc
      781 aaatactgtt caaatcactg ataaaaaaac gttcgatcaa gcagaaaaaa acgtggcgaa
      841 aaaaacagat ttgacgtatg agtccaagta taaagagaat acttatgaca tctactatcc
      901 aaaaaacagc aaggggcctg ttcctgttct attttgggta catggtggag gattcgtcgg
      961 cggagataaa tcgggtgtca aagaatttgc gacaaaatta gtttcagacg ccaacatcgc
     1021 agtggttgcg atgaattatg aattagcgcc tgattcagaa taccctaatc aagttcttca
     1081 agtgaatgaa ctgatcaaag atttattagc tgaagaaaaa gacgacaaat tgctagacat
     1141 ggaacaatta ttttttggtg gagatagtgc aggttcacaa atcgcattgc aatatgcagc
     1201 tatacaaacg aatgatgcgt atgctaaaca aatgaaaatc aaacagctac ttccaaaaga
     1261 tagtctgaaa ggaaccattt cttattgcgg acctgttgat ttgaagctaa cagcacagca
     1321 gcattcggat gatcgattta tgaagttttt cgtcaaaacc gttgcgtggt cgttgattgg
     1381 aacaaaagat tggaaaacga gtgagcaatt gaaagaagct agtgtggctg accatgtttc
     1441 taaggaattt ccgccaacgt atataactga cggaaatgca tactcattcc aggagcaagg
     1501 tcaagcatta gaaaatcggt tgacagaatt aaaggtaccc gtgcaatcct tgttttatac
     1561 agatacaaaa aaagagatta cccatgaata ccaatttgat tacacgctaa aagaatcgca
     1621 aaattgttac cagcagacat tggattttgt gaataagtat aagtaagagg ttgtgaaaaa
     1681 gccgtttcgt tttgagtatg aagaaacgaa gcggcttttc acaacctctc tttcaatacc
     1741 tttttgttct tgggataaaa ctttccctaa caatcaatcg tgcaggataa gtcaaaatga
     1801 ttggttcgtc ccgtataccg tcaattcgct ccaatgccat tcgaacagct gcttgcccaa
     1861 ttgatttcgc attgatttga acactggtca agccagggga gaggtttgcg attgtctcga
     1921 tattatcaaa gcttgctaca acaatctctt ctccaatttg gatatttttt tgtttcaatg
     1981 cccgatagac accaattgcc attggatcgc tcccgacaag aatggcagta ggaagagggg
     2041 attcattttc taatagatta gtcgcaagta cctcaccttg tttttctgtc cagccaccta
     2101 gatgataaga catatatttt tctaagtgat gatcgatcat aaaatgctga taggcacgca
     2161 tcctcttttc atttatagaa atctgcttcc caccgaattc atccatatcg actttatatc
     2221 ccccgatata ggctattctt ttatgaccat tttctaagaa caattccagt acggttttag
     2281 tcatccgttc aagatctgca tacaccatat cgacctcttg ctggatgtca gggttatcca
     2341 cgacgactat ttgcatgttt tgttttaaca aggcggtaat cgattctttc gtaacagtcc
     2401 caacaacgat caccgcacct aactgatcta gatcttccca ttgtttagga ttatcagata
     2461 gattatagat tcgattcatc cctaaatgca gacgtcgagc ttcttcttcc atccctactc
     2521 gcaaatgttt gtaatagata tcctgttctt cgctcgcttt gccgatcgta gagatcacac
     2581 caatcgaagg cattctcttt ttgatatatt tacgagtact gatgtcataa tccaacgcag
     2641 cagctgcctc tactatccgt tgccgagtag tctgagccac atggatcgtc tgatcgttat
     2701 tcaacacacg ggaaactgta gcaggagaga catttgcctt tcgtgcgaca tctctaattc
     2761 caaccatttt tacacactcc tcaaaaaaat aaaaaatttt tattgacaaa attgtttagt
     2821 aaacagtata caggaattat aaaaaaaagt aaacggtttc cgttttgttg ggagtgaaat
     2881 gatggatatt acgcatagaa aaacagataa aaatttcatc ttatttgatg aaaagagccg
     2941 agtcttccat ttacgaaata catttctttc ttatctgata aagatagaag aaagcaatgt
     3001 attggcgcat atttattttg gcaaaccagt caagcagtac aaagacaata aaaattatcc
     3061 ccgaagagat cgtggatttt caggaaatgt tcccttaaat cctgaccggt ctctgtcgaa
     3121 agatacacta ccgcaagaat actcttctca tggcagcatg gactttcgaa cgcccgcatc
     3181 gatcattcag cgcaagaatg gatcagactt actggatctg agatatgaca gccattatat
     3241 tacagatggg aagccagaga tcgaaggctt gcctcaaact tatgtgatgg acaagtcaga
     3301 ggcacaaacg ttggtgattt cacttaagga tagagaaaca gcgatttatt tcgatttgtt
     3361 ctataccata tttactgata gagcagtgat cacacgatct gtaaagataa gaaatgaaac
     3421 cggagaaaca atcaaactag aaaaagcggc tagtttccag ctagactttg cccatactag
     3481 gcgatttgat gaagtcattg ctttgccagg agcacatgtc aatgaaagac agatttcacg
     3541 gcaatctgtt ttatctggaa caaaagtatt tgaaagccgt cgaggaacga gcagtcacca
     3601 tatgaataac tttattgctt tagttcatca tcatacgaca gaaaatactg gagaagcgat
     3661 cggacttcaa tttgtttatt ccggcaacca ttctttcgaa cttgaaaaag atcagatcaa
     3721 tcaattgaga gtcgtcggtg ggatcaacag ccatcgtttt tcgtgggaat tgaatgcagg
     3781 ccagtctttt caaacacctg aaatgatcct ttcttactca agtcagggat tgaacaaaat
     3841 gagtcagatc caccatgagt tgctacgtga aagaatcgca agaggtcgtc atcaatttgc
     3901 tgaacggccg attctggtca ataactggga agccacttac tttgacttca atagtgaaaa
     3961 aatcaaggcg atcattgatg aagcaaagga gctaggcatc gaaatgttcg tattagatga
     4021 tggctggttt ggaaaaagag atgcggacaa ttcttcatta ggagactggt tcgagtatga
     4081 aggcaagttg acaaatggat tacgtgaaat agcagattat gctcatagca aaggattgaa
     4141 atttggttta tggtttgaac ctgaaatgat ctcggttgat tctgaactgt atcgaacaca
     4201 tcctgatttt cttatgcagg agccaggtag aatgccttct gcttccagat cacaacatgt
     4261 actggatttt acaaggttag atgttcggca gacgattgag aaacaaatga gaaagatttt
     4321 agatacgatt cctcttgact acatcaaatg ggatatgaac cgttcgcttt cagatgtcta
     4381 ttcgattacc cttgacccac agagacaagg agaagtagcg catcgttata tgctcgggct
     4441 ttatgaacta cttgaacatt tgacaactga ttatccggaa atcttatggg aaggctgttc
     4501 tggtggaggt ggacggtttg atgccgggtt tatttattat atgccgcagt catggacaag
     4561 tgataacacg gatgcagtgg agcgtatgaa aatccaatat ggaacaagcc ttgcttatcc
     4621 gatttcgtcc atcacagctc atgtatcagc tgtaccaaat catcagaccg acagatctac
     4681 ttcgttgaaa acaagaggag aaacagctat gagcgctgtt tttggttatg aactggattt
     4741 gacaaagctg tctcctgaag aaaaaaagca agtaaaagac caaattatat cctatcaaac
     4801 gattcgtccg gtgatccagt atggccatta ttatcgtttg gcgagtcctt ttgaagaaaa
     4861 tattgccgca tggatgtttg tgtctcctaa acaagatgaa gctattgtgt ttttagggag
     4921 gatactagct tcagctcaac cagcgttcca tgaagtatat ctgatggggt tagatgatga
     4981 ggcactttat caggaacaga cctcgaagcg gatattttcg ggggccgaat tgatgacagt
     5041 tggactttac ttccccgatt ttcaaggtga tttccaaaca gaactgcttc atttcaaaaa
     5101 gttatgagag agaaggaaaa aagtatgaaa gcaaatataa aatggttaga tgatccacaa
     5161 gtttttagag tgaaccaatc aaatgcacat agcgatcatg tttgttatgc aacaagggaa
     5221 gaagtagaaa ccacatcctc ccttaaacag tctttaaatg gatcatggcg tttttgttat
     5281 tcgccaaatc cttcggtacg accaaaaaca ttttataaaa aagattttga tgcctgttct
     5341 tttgatgaaa tcactgttcc tagtcatatt gaattagcaa attatgacaa gatccattac
     5401 atcaatacga tgtatccgtg ggagggacat gtatttcgcc gtcccccttt cacgacaagc
     5461 cctgagaaag aggcggaagg gatcttttca gctgctaaag acaatccggt tggttcgtat
     5521 ttgacgactt ttcgattaga agaaggtcta aagggaaaac aaatctcgat tcattttgct
     5581 ggtgtagaac aagcaatgtt tatttggttg aatggtcatt ttatcgggta tgccgaagat
     5641 tcgttcactc cgtctgaatt tgatttaaca ccttatttgg aagaagaaaa tattctagct
     5701 gtggaggttt acaaaagaag tacagctgca ttcttggaag atcaggactt tttccgattt
     5761 tttgggatct tcagagatgt cttcttagtt gctcgaggac aaaaccattt agaagatctt
     5821 tggatcaaac ctcgcttaga tgacaaatgt caaaatggca aacttgaagt agatttgtct
     5881 tttgagcaag tagccaaaaa cagtcagctg aatgtagcga tactagatcc tgatgggaag
     5941 gaaatctatc aacagcaata tccgttaaaa gagcaaatga cgattcgcat cgatggattt
     6001 gaaaatgtgg cactgtgggg gcattttcag cccaatttat ataagcttga attggagacg
     6061 atagacgaag aaaaaagagt aacggagtat attgactatt ccttcggttt tcgtaaaatc
     6121 gaaatcaaag ataaaatcat ctacttcaat ggaaaacgga tgattatcaa cggggtaaat
     6181 cgacatgagt ggcatccgaa aaaaggacgc gccatcacta tagaagatat ggaaaaagat
     6241 ttggctatca taaaaaatgc aaagatcaac ggcgtacgga catctcatta ccccaaccag
     6301 atcacttggt attatttatg cgaccaagca ggaatccatg tgatggcgga aacgaacctc
     6361 gaatcccatg gctcatggca aaaaatgggc gcatttgagc cttcttacaa cgtaccaggt
     6421 gcagtacctc agtggcaggc ggctgttctt gatcgagcaa aaacgaattt cgaaacatta
     6481 aaaaaccatc caagtattct tttttggtct cttggaaatg aatcttatgc ggaagaaaac
     6541 attcgacaaa tgaacgatta cttcaaaaaa gtagaccccg atcgacttgt ccattatgaa
     6601 gggaattttc caaatccagc attcgaagat tcgatttcag atgtaaaaag ctgcatgtat
     6661 gcgccaccag aagagatcat tgactacctt gaaaataaag cggaaaaacc gttcatctta
     6721 tgtgaatata tgcatgacat ggggaattcg ttaggtggaa tggattctta catgacgttg
     6781 cttgatcgtt atgaaatgta ccaaggaggc tatatatggg attttaaaga tcaggcgatc
     6841 caagtgactg atccaatcac aggcagagaa gtgatgcgct atggcggtga ctttgacgat
     6901 aaaccaagtg actatgaatt ctcagggaat gggatcgttt ttgcagatgg acaagaaaaa
     6961 cccgccatgc aggaggtaag atattattat gaaaaataca gtaaataaaa gtcatatgga
     7021 tacggaaaaa gttgcaatcg tcttcggcga ctgtacatta ggtgtcaaat cggggaatac
     7081 gcattatatt ttttcttata caagaggcgg actggaatcg ctcaataaaa acggaaaaga
     7141 atggctatac cgggaaacac ttccagtttt ttggcgagca acgaccgata atgacagagg
     7201 tagcggattt ggatttaaat ccggtatgtg gttaggagcc ggattgtatc caaaagtcaa
     7261 agaaatagaa gtactggtgg acaataaaaa gatcgatttg ccgattgctc cagtaaataa
     7321 tcaatattca agtaatgaat acgctgaaac ggttgaaatc atttttacac tggaaatcaa
     7381 cacagtacca aaaacccaag tgacaatttc ctaccaaatc gctggagagg gtaaaatgct
     7441 tgtcaatatg cattattatg gacaaaaagg attaccggaa ttacccgcat tgggtttaag
     7501 gttcattatg ccaacgaaag caaaattttt tgagtatgaa ggcctatcag gggaaacata
     7561 tccggatcgg atggctggtg gtgtcgaagg tatttataaa gtggcaggac tccccgtttc
     7621 cccatacctt gttccccaag aatgcggcat gcatatggaa acgaaatggg tagaactaac
     7681 aagaaatcaa acgttggata atacggataa agatcaagaa gatttttcgt tgcgatttga
     7741 acagcaggat acgccatttg ctttttccgc tttaccattc aaagcagaag aactggaaca
     7801 ggcaacacat atagaagaac taccgccaga aagacgaacg gttcttacga tttttgcaaa
     7861 agtaagagga gtcgggggta tcaatagctg gggagcggat gttgagtcac cttaccgaat
     7921 cagcagcgaa gaggaccatg aaatcggctt tattattcag tagcaatctt tagattatta
     7981 tttgaaaaac ggagttta
//
LOCUS       DAZAFF010000111         7806 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000111 DAZAFF010000000
VERSION     DAZAFF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.113"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1378)
                     /gene="celB"
                     /locus_tag="ACG9BB_002550"
     CDS             complement(26..1378)
                     /gene="celB"
                     /locus_tag="ACG9BB_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFN2838423.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVTLGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1378..1896)
                     /locus_tag="ACG9BB_002551"
     CDS             complement(1378..1896)
                     /locus_tag="ACG9BB_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838424.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1912..2238)
                     /locus_tag="ACG9BB_002552"
     CDS             complement(1912..2238)
                     /locus_tag="ACG9BB_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFN2838425.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2251..4230)
                     /locus_tag="ACG9BB_002553"
     CDS             complement(2251..4230)
                     /locus_tag="ACG9BB_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN2838426.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4251..4565)
                     /locus_tag="ACG9BB_002554"
     CDS             complement(4251..4565)
                     /locus_tag="ACG9BB_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFN2838427.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4733..5026
                     /locus_tag="ACG9BB_002555"
     CDS             4733..5026
                     /locus_tag="ACG9BB_002555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFN2838428.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5104..6216)
                     /locus_tag="ACG9BB_002556"
     CDS             complement(5104..6216)
                     /locus_tag="ACG9BB_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFN2838429.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6369..7217
                     /locus_tag="ACG9BB_002557"
                     /pseudo
     CDS             6369..7217
                     /locus_tag="ACG9BB_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7570..>7806)
                     /locus_tag="ACG9BB_002558"
     CDS             complement(7570..>7806)
                     /locus_tag="ACG9BB_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFN2838430.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2495 a         1553 c         1262 g         2496 t
ORIGIN      
        1 gtcacagttc cttttttctt agaaatcatt ttaacttttc actagctaat tctgctgcct
       61 tttctctttc ttcatttaat aatttattat catagaattt gataaatggg aaccagataa
      121 tgaatgcgat gattgcacaa atgactgcta acacagcagc tttccaatca ccaccactac
      181 caacaaatgc tccgatcccg actggtgaag gccacggcat ctgtgcaagc atcggtttga
      241 cgatttccag ccgaatggca aaataagcga gtgtagcaga agccatcgga gctaagaaaa
      301 acggaaccgc taaataggga ttgtagacga ttggcatccc gaaaatgatt ggttcattga
      361 tattgaataa tcctggtaca agtgaggctt ttcctaacac tttcagctga tcagatttcg
      421 cgaggaatgc aataaagatg atcaatccta aagtcgcacc tgatccacca acggttacat
      481 aagaattgtt gaattctcct gccaacggat aatttgcgcc ttgtgcgtta gctgccatat
      541 tagctaatac gatcggggtc aagaaagaag tgatgatatt cgctccatgg atccctacga
      601 tccacaatgc atggatcaag aaataaatca ccatgacacc aagccatgta ttcgtcaact
      661 gcgtaacaaa cccaaatggg atagcaatca ttttgaagat atccgttcct agtgtaacta
      721 ataatccatt gatgatcatc accacaaacg caatgacaaa tgttgggatc agagcagtga
      781 aagaacgaga aactcctggc ggaacaacat ctggcatttt tatgacccaa tttctcttga
      841 cacacataca atacaattcg accgcaataa tcgacataat aatagctgta aagattcctg
      901 aagttcctaa acgttcgaca aaagagccca tcctaatacc atttactaca gtttctgtct
      961 cattgataga agcaatcaag ctgattttac catcagtcat gatcaattca gggatacaca
     1021 taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg attaacattt aatttttgtt
     1081 catctcgttc aattgacgtt aattcataag caaacacaat ggcaaagtaa atagataaaa
     1141 tccccatcgt cattgtattg acgatcatgt acaagtcact aactttgaaa aaagttgcat
     1201 taaaaaatcc ttctaaaaat gggaacggta atggcaaaat atttaaaacc aaaaacatcg
     1261 agcctacaat cgtgaaagga atagaagcca tccccgctgc catgacagcc cgaacgattt
     1321 taagttgcgc aattttcccc ataggtccca tcaggtattt ctgcaaaaaa tcaaacatta
     1381 gttattctcc tttcctaaat ataacgcttt ttcatattgt ttaatacgga tatactgttg
     1441 gtcttggttg agtgaaattt tctttaaacg atactgtata aatgcactgg caaaaatacc
     1501 gcttacaaca agaatgaaaa caaataactg tcctccattg ttatttgcca taaaaggata
     1561 caagaatgta aatcctgagg taaacaacac tggaatcact aataaattaa agacactctg
     1621 gaacaaaaaa taccactttg tccatggaac gttcgtttgg tgttttcgat aaagctttac
     1681 ttgttctcct actgcaggca aaatagctaa aatcagaatc atcggtaaac tacttcctaa
     1741 acttttagct gatagaagca gaatcatcca ttgcatgttc atgaaaaaga aaaaggcagt
     1801 caggtaacgt acgattagat aacgattgaa atacatgttt ttcaaactaa gttgcttttt
     1861 ttctaattcg attttccttt ttactgtatc ctgcacaatg atccttcctt tctatttatt
     1921 tttttgatac aaataattca tttcctgtgc aacttccaat aatgtcatcg tcgtcatcag
     1981 atgatcttgt gcatggacca tgataatttc catttctatt tttgtcccgc tggcatattc
     2041 tttcagaagt tgagtttgtg actgatgcgc atgaagaatc tcttcatttg cttgtcgcaa
     2101 cttctcttct acttcttcaa acatctcttt acgcatcgag cgaaatgctt catgtatcag
     2161 tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa gtcacttgta gtgtttcact
     2221 gtttattttt tcagccatct gttttcctcc ttattcgatc agtttgataa atttactgct
     2281 gaaattctca aaaataggtt cagctaagat ttcctgctgt acttttagct ggtcaactaa
     2341 cctaacgatt gctttagtca cgaccgtaat tccttcattt tcgacataag aaggcgaaac
     2401 taaaaaaaca aaatgaattt cttgatattg atcccactgc atcccagaag gaatcaatgc
     2461 aacggctacc tttgtttcga ttccaactgg aacagcggga tgtgggactg ccactgtatc
     2521 actgaatatg atttgtccca tattttcacg ttgctggatc tgctttcgca tttctcttat
     2581 atatccttct tcttcattta tgctcagctt ttgcagcaaa tcatgaagaa tttcttcctt
     2641 tttcggttcg tacgtataag tcttgaaaaa tttttcagac aactgttcgg tgtaaatctg
     2701 ctttttttgc tgtagagaaa atgctggtat cggtctatct gaggaatgat agggtataga
     2761 ttctccaact acctttcgta ttttacgaac atcatcttca ttcaaaaaaa tactgacatg
     2821 taaaactgga attttgaaaa acattgtcga aagatcaata gaagaaataa tcaggtcgat
     2881 tccttttaaa gttgtttcat tgatctcata atagcctttt acacttacca ctgtaatatt
     2941 tttaccgaat tcactgacta cgcgattctt taatagttgg gcactgccgt aacccgttgc
     3001 acagataatc aatgcatgta gtttatgcgc agctctttct ttttccaatg ccgccatgag
     3061 atgcaaagcc aaataggccc attcatcttc attgatggta tagtttttca aagccggcat
     3121 gttgctgaaa tgccttttta ctgcgtcaaa gactttggga ttttctttct ttatctcctc
     3181 agtcaatggg ttctcaatgc gaatacctcg gctcaatcga atcatcatcg gtgacaaatg
     3241 atcgagtagt ccattgaaaa gctggagatc ctctactaat gaaaaatgaa attcctgaga
     3301 caatcttttc agaactttcg ctaattcctt tgatagttcc tgatcatttt gattttcttt
     3361 gtgattcgac ttagccatga gatgtaatgt caaataagca atttcttctt cgggaaacga
     3421 tacacctgcg attacctcaa tacgttgaac gatccgctgc gccacttgat actctactcg
     3481 cttagaaact tcttgttcga tgcctaattc cttgatttca aacccttctc ggagccgttt
     3541 gatccccaaa gccaagtgta aagtcagatt ctgtatgata aaatccgaaa cttttaactt
     3601 agcttctctt gtttcatcga gaatgataat gactagctct tcaaaactga tgtctgggaa
     3661 aaaacggctt tgccctaaat attctttcaa tgaatttcca taatggtttc caaaaaaagt
     3721 atccatgata aaatgccgtc gatttctttc ttccccttcg atccatgtcc cttgaccatg
     3781 cttagaaacg atttttagtg aatatggaat caatcgctcc ttgatttctt gcatgtcctt
     3841 tgtcaaactg gaacgactga tataaagctc ttctgccaaa tcatccataa aaagaatggc
     3901 atcttccaaa agcaattggt tcaatatata cttttgtcga tcttcggact cataaaatcc
     3961 ttgcggttct ctagcatatc caggtactat ttcttgccga ctcaaaaaca agtcaaatgt
     4021 cagtctttgt aaaatatgca gttggtagcc ataacctggt ttagcaatga tcttacctcc
     4081 gtttttttct actaactctt tgatccgctg caagtaatta cggattgtcc gatcagataa
     4141 taacaattca gaagctaaac gttgactggt gacatactcc ttcttgtgat caatcaaata
     4201 tctcaacagc aatttttcct tctgtttcat tatcatccac tcctattcca tcatatgttc
     4261 tcttcgatca cctctgctaa cttctggatg cctgtaggga ttgggacata cgcttggaaa
     4321 gggatactaa caacaggctt tccaacagat tctcctaatt ttttgaattt atccaaaaac
     4381 atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac tattcttaat catattatcg
     4441 ccttctgtag ctgaaactgc atctacgagt acctcttctc ttttttgctt aaagaaatcc
     4501 gttgtctttg aggccatcat cgaggatgac attcctgccg cacaaataat caatgctttt
     4561 ttcataaatc aatcactcct tttattttta taaaccaagc atatcttgta agcgttttct
     4621 attcaaaata tatatttccg tagcattgcg gaaaaaatgt gtatcatatc ttgaataata
     4681 atttctggca ccgtgatata ctagtgatag aattttttca ggaggggatt tagtgagtat
     4741 cacagtgaat attttgtata caagtacaaa tggacaagcc agaaaatttg cagaagaaat
     4801 ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa ggaaacgaga aatatgacta
     4861 ttactttcca ctagaagatc cagaatctct tttactgatc gaccgttgga aagatgaaga
     4921 agcaatcgaa aagcatcaca aatccgaaat gatggctcaa ataaacgaac tccgtaaaaa
     4981 gtataaatta aaaatgaaag tcgaacgttt cactgattta ccgtaaaaag atacacatgg
     5041 aatgatagca atttatcatc catgtgtatc ttcacttttt ccttattttt tattcgatct
     5101 catctaatga ttcaaaccgt gtttttctct tatttttcgg ataagtattt cagtcacccc
     5161 aatatacatg attcctaaac ataccccaag gatattctca aaaatgcgga tcgttactgc
     5221 tcctggaacc ccgaataaac tggcagtgat tgccaaagca ccaaaagaat taaaaactgt
     5281 tttatatcta taggtagaac aaacacctaa agctagacca cctaatatac cgacccagtt
     5341 aaaaggaata aattggctga taccaataaa taaaacacat ccaatcaatg agccaatgat
     5401 tcggtctact gctcgttctt tgaaaccaat cgacatcaat ccatagctag atacaataga
     5461 agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg acatactctc ctgcaacgat
     5521 caaaagacta atgccaaatg catagatgag catccaaatt ttttcctgcg aatacatgcc
     5581 tttcaaaaaa atctctttaa acaagctttt gtctttgttc ttttctcgat gttttctgta
     5641 aaggagaact gaaaaccaac aaaaaaacaa aaataataag ctacttgttt gtgtaaagaa
     5701 attcttgttc aacgaatctt taggtaacga ataaacaata aagagatatg aaaagccgta
     5761 caaagaagca ttccccattt tgggatcact agcggttgcc attaatatgc tgcttagtaa
     5821 aataaaatga atcaacatgc ccataactga ccatttgatc agctggacat acggtgctac
     5881 taatagtgag aacatgacga ttgctaaacc tatgatcgaa tgggaaatct tataaccaaa
     5941 gtcaacaaaa cgaatactca tcaacataca gaataaaacc acagcgtatg gtttatgctt
     6001 cgattcaaat aatgttgtaa ttataatgat aaaacaaatg gcaaacaata cgatgaataa
     6061 tgatcgtaaa aacaatgaac aaatatacat gcacttttct tttttagaag ctgcttctcg
     6121 tattttttgt tttaaaataa acggatctag ttgtagagac tgataaaaat ccacctgttg
     6181 tcgtcctttc tttttacttt tattttttac agtcatctat gacaggttgt aaatactatg
     6241 gaacatcata tggcgttgtc attttcttgt caactatttt tcctattcat gtttttataa
     6301 aaaaacatat cgttttcttg tatgattaaa tcaccacaaa ataaacaact gagagaacga
     6361 tatgctttat gaatggttct atcaaaaaaa ttctgagatc aatagacaaa aatttgatga
     6421 ttacagaggt ctcttacgaa accttacaga agaaaaaaac attgatcgtt gctgcagttc
     6481 tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc tgtggtagat ggaaatagga
     6541 aattaatggt ggtgaaaaac aagaagaagg aaaccattgt ccactttgaa taatataatc
     6601 atatgccaat ggttatacgc ctaaaaaagc agcgctatac ctgtaaaaac tgtcgaagcc
     6661 attggaacgc tcaaagttat tttatccgtc caaggcactc gatttcaaat catgtcagac
     6721 ataaaatcac ttctttactg actgaaaaag tgtcgttatt ttttatttcg aaaagttgtt
     6781 aggtatcttt gacaaccgtt attcgcacat tgaaagagtt gagaagctat ttaccaaagt
     6841 cttccaaaag aattctgtct tgagtgttga gggttgtgaa tttcgttctc acgtttccgt
     6901 tgaggataaa ctgagtttta tttccgcgga tggcgagaca gaaaaattaa tcgagaaaat
     6961 ttacctacac aaaaaattcc tcgattaaca aactatttcc ttagctgcac taatcctaaa
     7021 gaaatagaat tcttagttac agacatgaac gccacctact ttcagctcac caaacatgtt
     7081 ctaccaaatg cgaaattggt gattgatcga ttacttggtt tttccacgcc tttcaaggag
     7141 gcgtaacctt tattcacgag ttggttgaag ctttccgaga caaaagaccc gacttgtttt
     7201 tctccttatt gacaaagagc cattaaaact agagaaaaaa ttacaactga tcgctgatta
     7261 tttgtagaaa gaaaatcaaa gtattaccca ataaacttga gtcattaggc aaaaaacact
     7321 gcgaaactca agaatgattt tcgcagtgtt tttgtatatt ttttattaac tagtattact
     7381 acaaaggttt atttttcact tctcaacgca gcacaaaatt gtctttactt atttactggt
     7441 tgggttcaac cttctctact ccgatactaa tatttgtttg tatacccctt ctaaaatcgg
     7501 tagtttccat tcaattgatg ggtactagtt gattttaaaa actatgctta ttcaattttt
     7561 tattttagct catatatttc gtttctttct aaattttata ctgatgaaaa caagtagcac
     7621 acccaatatt gacaatataa actggctaat gatggtttct ccagtttttg gaagatgctt
     7681 atttccactg cttgttggat tgtttcctct tatatctgag ctatcagcaa cttttctatt
     7741 ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg atcttgaaag ttactggtgt
     7801 cgcatc
//
LOCUS       DAZAFF010000112         7759 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000112 DAZAFF010000000
VERSION     DAZAFF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.114"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..107
                     /locus_tag="ACG9BB_002559"
     CDS             <1..107
                     /locus_tag="ACG9BB_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFN2838431.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="ACG9BB_002560"
     CDS             complement(176..904)
                     /locus_tag="ACG9BB_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFN2838432.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="ACG9BB_002561"
     CDS             complement(917..2197)
                     /locus_tag="ACG9BB_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFN2838433.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="ACG9BB_002562"
     CDS             complement(2338..2940)
                     /locus_tag="ACG9BB_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFN2838434.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="ACG9BB_002563"
     CDS             3185..3391
                     /locus_tag="ACG9BB_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838435.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="ACG9BB_002564"
     CDS             3703..4422
                     /locus_tag="ACG9BB_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFN2838436.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="ACG9BB_002565"
     CDS             complement(4503..5186)
                     /locus_tag="ACG9BB_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFN2838437.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="ACG9BB_002566"
     CDS             5405..7591
                     /locus_tag="ACG9BB_002566"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFN2838438.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2782 a         1308 c         1465 g         2204 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaactaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga ggtgtgacaa
     7741 gaattttgtc acacctccc
//
LOCUS       DAZAFF010000113         7601 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000113 DAZAFF010000000
VERSION     DAZAFF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.115"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACG9BB_002567"
     CDS             complement(138..428)
                     /locus_tag="ACG9BB_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838439.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACG9BB_002568"
     CDS             complement(546..806)
                     /locus_tag="ACG9BB_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838440.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACG9BB_002569"
     CDS             complement(943..1356)
                     /locus_tag="ACG9BB_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFN2838441.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACG9BB_002570"
     CDS             complement(1812..2297)
                     /locus_tag="ACG9BB_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838442.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACG9BB_002571"
     CDS             complement(2436..2651)
                     /locus_tag="ACG9BB_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFN2838443.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ACG9BB_002572"
     CDS             2875..3675
                     /locus_tag="ACG9BB_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN2838444.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ACG9BB_002573"
     CDS             3694..5562
                     /locus_tag="ACG9BB_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFN2838445.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ACG9BB_002574"
     CDS             5555..6046
                     /locus_tag="ACG9BB_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFN2838446.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /locus_tag="ACG9BB_002575"
     CDS             6034..6588
                     /locus_tag="ACG9BB_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFN2838447.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..7601
                     /locus_tag="ACG9BB_002576"
     CDS             6606..7601
                     /locus_tag="ACG9BB_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFN2838448.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DAZAFF010000114         7597 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000114 DAZAFF010000000
VERSION     DAZAFF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.116"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..553)
                     /locus_tag="ACG9BB_002577"
     CDS             complement(41..553)
                     /locus_tag="ACG9BB_002577"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFN2838449.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(567..2864)
                     /gene="recJ"
                     /locus_tag="ACG9BB_002578"
     CDS             complement(567..2864)
                     /gene="recJ"
                     /locus_tag="ACG9BB_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFN2838450.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3006..3449)
                     /locus_tag="ACG9BB_002579"
     CDS             complement(3006..3449)
                     /locus_tag="ACG9BB_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFN2838451.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3450..4238)
                     /locus_tag="ACG9BB_002580"
     CDS             complement(3450..4238)
                     /locus_tag="ACG9BB_002580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFN2838452.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4235..5176)
                     /gene="rnz"
                     /locus_tag="ACG9BB_002581"
     CDS             complement(4235..5176)
                     /gene="rnz"
                     /locus_tag="ACG9BB_002581"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFN2838453.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5353..5940)
                     /locus_tag="ACG9BB_002582"
     CDS             complement(5353..5940)
                     /locus_tag="ACG9BB_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFN2838454.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6161..7474)
                     /gene="obgE"
                     /locus_tag="ACG9BB_002583"
     CDS             complement(6161..7474)
                     /gene="obgE"
                     /locus_tag="ACG9BB_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFN2838455.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
BASE COUNT         2046 a         1784 c         1299 g         2468 t
ORIGIN      
        1 ttctttaaga ataacatcaa gaatagcttc ttttttctgc ttaatattcc attaatgtaa
       61 tgatgtcata tcctttgatt ttgtcgcgtc catggagatc catcaattcg atcaaaaatg
      121 cgcagcctac gactacaccg cctaattgtt cgatcagttc gatcgtcgct ttgattgttc
      181 ctccagtagc tagcaaatcg tcacaaatca gcacacgttg acctggttta atggcatcct
      241 tatgcaatgt caatgtatct gttccatatt ctaatccata agtcacttcg atggtttctc
      301 gaggtaactt cccttttttg cggactgggg caaaaccgac tcctaactca taagcaacag
      361 gacatcctac gatgaatcca cgggcttctg gcccaactac catatcaatt cgtttttctt
      421 ttgcataatt tacgatttgt ttagtagctt cccgataagc gtcaccatct gccatcagtg
      481 gggaaatatc tcgaaacaca attccttttg acggatagtc gggaatactg gcaatataat
      541 cttttaaatc cactctttgt ttcctcctag gttgttaacc atttctttat ggtaggaata
      601 tcgctcaaaa gcaaaaattc ctcaactttg attttattga gccgtcgctg gtataatctg
      661 ctctcagtca aaggatgact ttctgggcta gcgacttttt gtaaaacacc gttctctatt
      721 gtaacaaatt tcaattcaaa aaacacctgt atcataaaaa ttaataattt ttcagggata
      781 cgcaaatatt gagctattgc ttttatttta tgacgtatat caattcgttc ttgagaatgg
      841 atcaatttaa acaattttgc gtactgttct cttgtgccta ccccattcaa ataggcctca
      901 tcaggtgaaa tccccatcag ataaacacga ttgaatgaat aagaccgaaa aacttctttc
      961 agatccgcta aatcatctgg acaatccata acaactagag agtttggttt tcttttttca
     1021 attattgcct ttacagaatc tagtccttcg aaaacaatcg cagcttcctg gataatctca
     1081 ggcacgaatt tcaacgtcga agaatcaaat gctaaataaa ggctaccttc cgacgttcgg
     1141 ctctgttcac ggaaacgttt tgcccgccaa tcaaatactt gcagaccacc cacagaaaaa
     1201 tcagatacca tcagctgcgg tttcttacgc ccgttccatt cattgatcga aagctttcct
     1261 accacatcta cctgattatt cagcagttct tcttcttgag taccaaaacc aaaagcgacc
     1321 gcatcaagct gactcgatgc atcggaaagg acaaatttca gatgctgttg gtttgctccg
     1381 attcttttga tattttctgc ttggacttgt cgaaataaaa actgaggcaa aggattatcc
     1441 gtaccaaatg gcgccaatag tttcagctca tcaattcgct ctactgttac ttcatttggc
     1501 agcaatactt catcaattct caactcaggc cctctcatca agtcaatttg atgatcgact
     1561 atatattggt tcatcttctc ctgtagtata gtgacatttt cacttggcat cgttaaaccg
     1621 actgctgcat gatgtcctcc gaagtaagta aataaatctc gcatctggtc aagcatttca
     1681 aaaagattca aggcttcaat actacgtcca gatcctttag ctgatccatc ttcttttaaa
     1741 gtaaggacaa gcgttggttt acccgtttca ttcattattt tcccagcaac aatgcccaaa
     1801 acgccttcat gccatcctcg gtttaccaat agatggatat gattctcctt attgaccatc
     1861 gctaatgctt cttgtgttat ctgctctacg attgcttttc tttcttcatt gatctcattc
     1921 aatttttttg cttgaacgga tgcttcctct tcatcaaacg tcgcaagtaa cgagacggca
     1981 ggacgtggat cgcccatccg accgattgca tttagtctgg gagcaatcga aaaaccgatc
     2041 gttgtttcgt ctgcgtccct catttttacg ccgctttctt caaatagcgc ctgaagtcct
     2101 atccgttctg aatgatgaat ggcatcgatt cctaatgaaa caaggattcg attctcatca
     2161 gtcaatgaaa ctaaatcggc gatcgttcct atcgctacta aatccaaaaa ttctgcaggc
     2221 ggttcttcca ataatgcaga agctactttg aatgctactc cgactcctgc caaatctgga
     2281 aatggatagt gccccgctgg atgacgaggg tgaacaatcg cataagcgtc tggtaaattc
     2341 tcaggtagtt cgtgatgatc tgtaatgatc acgtctacac cagcttgctg agcgtatgct
     2401 accgcatcat ttcctgctac cccgttatca acagtgatga tcaactggat tccttcttcg
     2461 atcttttctt ggtataccga ttggtttggt ccatacccat gctcaaatcg atttggcagg
     2521 aatacttcta cttctgcacc aagtaattcc agtgtttctt tcattaccgt tgcactggtg
     2581 atcccatctg cgtcataatc accgtaaacg aggatttttt ctccactgat tacagcttct
     2641 tggattcggc tgattgcttt atccatatcg tgtatcagat acggatcatg taattcttgt
     2701 tcctttggat gtaaaaatcg gttgatatcc ttttcctcat ggtaacctct ttgccacagt
     2761 aacttaccga caaatggaga taatttcagt tcacggatca tttggagaaa ggattcagaa
     2821 ggctctgtct cgcttaactt ccaatcaaaa tttgcttgtc tcactttctc actcctaact
     2881 tagttattat agcaaaggaa atcagcaaaa aaaagcagaa agaaagaata aaaacagatc
     2941 agatccgcta gacagatgtt cagcaggacc tgatcttttg tacgaccatt tatgaaatat
     3001 ccctattagt caaaataatt gaagctttgt ttgggcgatt gagttttttg ttcttccggc
     3061 gctttcaatt gactgatttg tgcggttttc gcttgtaact ccgcctgata agcagcttcc
     3121 aactccgcac gttcgtttct aaactcgcgt tgctgttctt cttttgcttc ttcaattttc
     3181 ttttgaagtt ctgtttgata ttctgacatt tcctgagtca gctgtttgat ttgctttttt
     3241 tgctgccata gtgttgccga agcagtcaaa aatacaatca gtgcaccaat cagcgcagaa
     3301 ccgatgatca ctaagatcaa tggcgcagaa aaggaagtga agccaaaact tacaggaaca
     3361 tttttattgt tcaatacagc aaaaagaaca ataatcaaaa ctaaaacaaa tccaagaatc
     3421 acattgcttt gctttttcaa ttccttcacc tactttttgt taaaaattcc gctggctaaa
     3481 taatcaccaa tcgttggaaa cagcgtataa aattttgatg caacttctaa aataaacggt
     3541 tgattgattt cccgttttgg atgtctcata ttgcggacga tcatttttgc caacttggca
     3601 ggttctaaaa cgatttgtcc gactttttcc aaatagcttc cgcttggatc agccttatcg
     3661 aagaaatttg tctcgattgg acctggattc actgtcgtaa cagctacgcc aagtggtttc
     3721 aattctaatc gcaaagcgtt ggagaagcca aggactgcaa atttcgtggc agaataaacc
     3781 gttgattttg cagttgccat ttttccagcc atagaggcga tattaatgat atgaccttgt
     3841 cttctttctg ccatatcgat cgcaaatttt tgtgtcaaaa ccatcattcc taagatgttg
     3901 acttcaaaca tatcgtatgc ttttccaagg tcgaatgtaa ggaagtcttc gaaaatccca
     3961 aaacccgcat tgtttaccag aatatcgacg cgcccgactt cttccatcac cttttcatat
     4021 aattcgtcca cgctttcagg attgctgacg tctaccttga aagcataagc ctctttgccg
     4081 ctcaagcgtc tgcactcgtc tctgacacct tctacaaatg ccattcttcg tgcgcaactt
     4141 ataacgattg ctccttgctt tgctgcttca taacagattt gtgcgcccag accgcctgaa
     4201 ctacctgtta ctaaaacaac tttatcttct aaattcatgc ctcttcctcc tcgcgaaatg
     4261 gaatctcaat gctatcaaaa tctttcataa tcgtcgtgtt ttcaaacact tcttgtgctt
     4321 ctttttcaag ctctaaagct gcttttccta aatatctcgc actgatatga gtaagtagca
     4381 gccgttttgc ctgcgcttct tttgcaactt cagcagcttg atgagtcgtg gaatggaaat
     4441 agttgtgcgc cattcttgct tcatctttat taaatgtgct ttcatgtacc agaatatcgc
     4501 tattttccgc aagcacaacg gaattatgtg tttttctcgt atcgcctaaa atagtcacga
     4561 ttcgtccttt tttatctgga cctacgaaat cttttccatt gatcgtccgc ccatcttcta
     4621 gttggatcgt ttcaccttgc ttcagtttgc cgtacagtgg tccagctgga atacctaatt
     4681 cttttagacg atccacctgc aattctcctt tatgatcatg ttcaaccaca cgataaccaa
     4741 aactgtcgat cccatgattt agtttttttg tataaacaga aaattgctga tcagtaaaaa
     4801 tcggggctgt ttcatttagc tcaatgaatt tcagcggata acttaaacgc gtttgcgaaa
     4861 tacctaaaga gacttttatg tattcctcta tcccttttgg cccgtaaatc tctaatggcg
     4921 tatccccacc ttgaaatgac cggctgctga tgagcccggg aagtccaaaa atatgatcgc
     4981 cgtgtaaatg tgtaatgaaa attttaccga tttttctcgg acgaatagtc gttcgcaaaa
     5041 tctgcatttg cgtgccttcg ccacagtcga acaaccaaac ttcattgcgc tcgtctaaaa
     5101 gtttcaatgc tatacttgtc acatttctat gttttgcagg aacacctgca cctgttccta
     5161 aaaattgtaa ttccataatc tacctgactt tctttaagtt cctctttaat tttagggtaa
     5221 aaagaaggga taatcaaccg taagtcgaga atgggttcaa attaaaaata acgttctaaa
     5281 gcgatagcgt tttctttgaa aattactata aaaacagcag cgaacccgtt tgagatcctc
     5341 tgctgttttt tattaatttg aagatccaga actttcattg tcgttggaag tttgcggatg
     5401 tttgtccttc caagtctttt gtgcagcttc tttcgcatga ttttcagctt ctttcgcatc
     5461 atgcacatct ttttcgacat cttcataatc caagcgagta atttctccgc ctaattccat
     5521 cattaccaat tgattcagtg ggcgattgtc ctcttcatca gcaatcaaga tcagtcctgt
     5581 agctccttca ccgattttat ttcctacaaa gtcgaacacg gtctgggcat cttttatttc
     5641 tttggcatct tgtgtcgcac cgatcattcc tccggcaaac catcctaata aaatccctag
     5701 cggaccacct aatacaccaa ccaacattcc gattaatcca cccgtagaag atttgttatt
     5761 accggtaaag tcgataaaat cttctatttt gaattgatgt gtcccattag gatcatggct
     5821 gacaactgcc atctgttctc ctttaatcga acgaacagca tgtaactttt tgatttctga
     5881 aaatgcctga tatgcctggc tttctacttg aaaatttaaa atgattactc gtttgctcat
     5941 taaaaacgcc cctttcatac cttcttgtta tttattttac ctttaattga aaaataagaa
     6001 aagaaaagca ctcttttttt ccaataaaat agggctgtga caaaagtcgt atcacagctc
     6061 ctttttccga ataaacggcg ttcttgaagc tgatcacttc tgtcacaacc tcccttaaat
     6121 ctgaatctat attttttggt atttgttttt atttgcttgc ctattcgaca aattcaaatt
     6181 caaactcacc gatacgtaca aggtcgccat ctttcgcacc gcgtgcccga agagcttcat
     6241 ccacacccat accacgtaat tgacgagcaa aacgcatcac tgtttcatca tgatcgaaat
     6301 tcgtcatttg gaataatttt tcgatcttct caccagataa gatccacgtt gcatctgaat
     6361 cacgatcgat ttggaactca ggaccttctg gattgaatcc atagtgtaca gtttcttctt
     6421 ccagttcttc ttcgtacaat gggaattctg gcgttacttc taacaaatct gctgtagcat
     6481 tcaataacgc gtccaatcct tgtcgagtca ctccagaaat tggaaatact gggatgtcat
     6541 cagcaaattc atcttctttt tccttgttca gctgttcttt gaatttcact agattttccg
     6601 gtgcatccgg catatccatt ttgtttgcca cgatgatttg cggacgctct agcaagcgta
     6661 agttataagt agataattct ttgttgatgg caagatagtc ttcataagga tctcgtcctt
     6721 ccatcccact catatcgatc acatgtaaaa ttactcgtgt tctttcgata tgacgaagga
     6781 attgtgtacc cagcccaaca ccttgcgaag ctccttcaat caagcctggc aagtctgctg
     6841 cggcaaaact tctgccatca cttgtagtaa ccattcctag attaggtacg agcgtcgtga
     6901 aatgataagc gccgattttt ggacgagcag atgaaataac ggatagtaaa gtcgatttcc
     6961 cgacagatgg aaaacctaca agtccaacgt ctgccaatac tttcaattct agttcgattt
     7021 ttcgctcttg tcctggttca ccgttttcag caatttccgg tgcaggattc cgaggagagg
     7081 caaaacggat attccctcgt ccaccgcgtc caccttttgc cacaactagt gtttgaccat
     7141 tttcaattaa atctcctaaa agagcaccag tctccgcatc tcggacagtg gttccttgtg
     7201 gtactttaac ataggtatct tctgaaccac gaccgtgcat ccctttactc atcccatttt
     7261 cacctggctg cgctttgaaa tggcgattga accgaaaatc catcaatgta cgtaaccctt
     7321 catcgacgat caagatgaca tcgccgccac gaccaccgtc tccgccagcc ggtccgccgt
     7381 caggaacgta tttttcgcga cgaaatgcaa ccattccgtc tccgcccttg ccagctttca
     7441 catcgatcgt tacttgatct aaaaacatgg acataattaa gtcctcctat tattcattta
     7501 tttatttgac tgcctgaatt tccacaataa acaaaagtct tacttatttt atcggtgaaa
     7561 tctgattttg tctatcattt cagcaaaaag attgttt
//
LOCUS       DAZAFF010000115         7396 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000115 DAZAFF010000000
VERSION     DAZAFF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.118"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACG9BB_002584"
     CDS             complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACG9BB_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFN2838456.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACG9BB_002585"
     CDS             complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACG9BB_002585"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFN2838457.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2298..2960)
                     /locus_tag="ACG9BB_002586"
     CDS             complement(2298..2960)
                     /locus_tag="ACG9BB_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFN2838458.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3144..4790
                     /locus_tag="ACG9BB_002587"
     CDS             3144..4790
                     /locus_tag="ACG9BB_002587"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFN2838459.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4781..5863
                     /gene="uxuA"
                     /locus_tag="ACG9BB_002588"
     CDS             4781..5863
                     /gene="uxuA"
                     /locus_tag="ACG9BB_002588"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFN2838460.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5971..7396)
                     /locus_tag="ACG9BB_002589"
                     /pseudo
     CDS             complement(5971..7396)
                     /locus_tag="ACG9BB_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2314 a         1361 c         1367 g         2354 t
ORIGIN      
        1 tcttgtcacc atcctttttt ccgctctctg actttttaca atcttcatag ctcaagtgcg
       61 catcagataa tcgaatgctc ctaaagctgc agtagctcct gaacccattg aaacaatgat
      121 ttgtttgtat ggactatctg tacaatcacc agctgcgaaa acgccgggga tattcgttgc
      181 gccatttttc tcggtcacta tttcatttct tggattcatt tcgatcgttc catctagcca
      241 atccgtatta gggagtaagc cgactagaat gaatactcct gaaacagcta gtgtatgcat
      301 ctccatcgta cggcgatcga tataggagag agattctacc tgattcgtac cgctgatttc
      361 tttcgtcgct gcatgtttga taacagtgac attcgcaaga gagactagtt tatcctgtaa
      421 gatttgatct gcttttaatt ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc
      481 agccaaatcg attgctgctt caacaccaga attaccacca ccgataacag caatctcttt
      541 tcctttaaat aaagggccgt cacagtgtgg gcaataagca attcctttat ttttaaactc
      601 tttttcgcct ggtacattga tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt
      661 tgctttaagt gatataccgt tagctaagcc aatattaata aaatgttgct ctttatttaa
      721 ggaaacggcc tgttgattca tcataatgtc gatagggtaa ctacgcacat gactttctac
      781 ttgctgcatc aattttggac cttctgtgta agaggtacca atcatatttt caatccctag
      841 tgtttcaacg acttgtccgc cgaaagaatc agcgacaagt cctgtacgga ttcctttacg
      901 tgctgcatag atagctgcac tactagctgc tggtccaccg ccaatcacta atacatcata
      961 aggttctttt tcaaacaact cttcttcaat caaagggcca gtgatttttt caacaatttg
     1021 ttcgatcgtc atgcgtccgc tactaaattc ttctccattt aaaaacacag ttggcactgc
     1081 catgattttt ttatcatcga tttctttttg gaacatccca ccttcaatca tcgtatgagt
     1141 aatatttgga tttaatacag ctaatatatt caatgcctgt acaacgtcgg ggcaattatg
     1201 acaagtcaag ctaacataac tgtcaaaatg caatgtttgt gttatttttt tgattttaag
     1261 actcaaagat tcactgattt tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa
     1321 agaggaaaat tcatgaccga gggggatacc agcaaaaacg attccacttt ctttgccaac
     1381 tggattgata gaaaaactag gtgtgcgcat cgaatctctt ttttcccaag agattttagg
     1441 agacatttct gctatttcct tcgtaaattc agctagtttt tgactatttt cactattatc
     1501 caaactaaca gaaaaaacaa taggagattc aatcagtgct aaatattgtt cgagattttg
     1561 tttagtttct ttgtctaaca tatccaccac ctaaatcttt ccaacaagat caaaacttgg
     1621 tttcaatgtt tcgccacttt ctttccattt tgccggacaa acttcacctg ggtgggtacg
     1681 tacatattga gcagcgcgaa tcttatcaat cagtgcacta gcgtcacgac cgattccatc
     1741 tgcattgatt tccatagctt gaacgattcc gtctggatca ataataaatg ttcctcgctg
     1801 agctaatcct tgctcttcat ccaaaacatc aaaaatacga gaaatctgat gagacggatc
     1861 accgatcatc acgtattcga ttttaccgat agcatctgat gtatcatgcc atgctttgtg
     1921 agtaaagtgg gtatcggtag agcaagaaaa gacttcaaca cctaaagatt tcaatgtact
     1981 gtattgttct tgtaagtctt ctaattcagt aggacaaaca aaagtaaaat cagctggata
     2041 gaagcaaaga atactccagt tccccataag atctttttca ctgacttcta aaaattctcc
     2101 ttgatgataa gcatgtgcat taaattcttc gatttttttc ccaatcaatg acatagtaaa
     2161 acctccaata ataattattt atttataatt attgtaattt agttggagtt gcaaagtcaa
     2221 tggatatttt gttatttaat aaagattact caaaaacgaa ggttaattat aaaaatagaa
     2281 gaggtcgcaa gaaaatgcta ctgctggaaa tactcgggat aacgttgagt aacagtttcc
     2341 tgttctttca gcatcatgtg taagtgcaat cccgtttgaa atcgagcttc ttctggtttt
     2401 ttatcaacta aagccttgag taaatcataa tgttgttcca tgatcgtttc ccatggaagc
     2461 ccctgaatgg tcactcgtaa ccagcggaaa cgttctaaat gggtattatg gtcatctaac
     2521 cagttccaaa cttcttttct tcctgcaatt tcgaaacaag cttcatgaaa taaattatct
     2581 gcacggaaaa aagcgcgttc gtcatttatt ttgactgctt tttcttgttc ttctaaaatc
     2641 gtttctagca ctattcgtgt ttgtgtgtcc aatttggaac aacattcaat catgatttgc
     2701 tcttccaact gttctcggac aaagcgagca ttttctgcag aaacaagatt aattttggaa
     2761 acatacgtac cactttgagg tatcgtatac acgagttctt gctgacgtag ttgaatcaat
     2821 gcttcgcgaa tgggagtccg gccgatagca agatgttcct ccagtgcctt ttcagatatt
     2881 ttccttccag gttctaagtc agcatagata atttgtttac gaatcgcttg ataagcttgt
     2941 gattgcaagc tgcttttcat ctttattcct ccaaaatcat ttctatgaac gtagtataac
     3001 agaagaggtc tgaaaaacaa tctggtatgt cacattaact agtaaatttg tataaatagt
     3061 aaaattttgg ttgacatagc gatttttatt gcataaaatt cacttgaata caaaaactaa
     3121 tatgttagtt taggagggga aaaatgattg atttaaacaa ctggaggaac tttatccctg
     3181 aattatctca aaaagaagta gaaatacctc attttgaccg tgcatcatta agagaaaatg
     3241 gacaaaaaaa tcctgtctgg atccatttcg gtggcggaaa tctttatcga ggatttcatg
     3301 cagaaatcgc tcaacgttta gcggatctag gcgaattgtc cactggtgtc attgtttgtg
     3361 aaacattcga tgaagaaata attgaaaggg cttaccaaga ttttgatgat ttgattcttg
     3421 aagtggtcat gccaagcagt ggagagttcg tcaaacgtat attagctgcg acagctgaca
     3481 gtctgtattg tcatccagat tgttctgaac actttgataa gatgattcga cactttgaaa
     3541 atcctagttt acaattagtt acttttgcta ttactgaaaa aggttatcga ttgaaagata
     3601 caaataataa atatatagga ccgattgcag tcgatataaa aaatggtcca aagaagccat
     3661 cacatacaat gagtattctt gctgtgctac tttataaacg ttttttatct ggagcttggc
     3721 cgattgcact tgtctctaca gataattttt cacaaaacgg tagtcaatta cgtacttctt
     3781 tgctaacgat cgcaaaagaa tggcaaaagg aaggttatgt acaaaataca tttatccaat
     3841 acttatcgaa tgaagaaatt gttgcgtttc catggacgat gattgatcgt atcaccccga
     3901 atccttcctt gtcagttagc aaaatgttgg caaaagaggg cttttcaaat atgtcgattt
     3961 tccaaaccgc aaaaggaaat aatattgctc catttgtcaa tacagaggac atccattatt
     4021 taatcatgga agatcatttt cccaatggac gtccgaaatt cgaaaaagct ggggtattct
     4081 tgactgatcg tgcaacggta gataaagcag atacgatgaa ggtgacgact tgccttaatc
     4141 ctttgcatac gtctttagct attatgggtt gtttactggg gtataggagc atagctgagg
     4201 agatgaaaga tccagattta tcgctgctag tttctgaaat cgggtacaat gaaggcctcc
     4261 cagttgtgga gtctccaggt atcattgatc caaagatctt tctggaagaa gtaattactg
     4321 tgcgcttttc taatccaaat attcctaata tgcctcagcg aatcgccacg gatacctctc
     4381 aaaaggtggc gattcgttat ggtgagacga ttaaaaaata tatggaaata gaagttaaag
     4441 acccgcagac acttgtattc ataccattga ctctcgctgc ttggcttcga taccttattg
     4501 gcattgatga tcaaggaaaa ccattcatcc cgagtccaga cccactattg acagaccttc
     4561 agcataagct ttccgatatt caactgggta cgcaagatac atcacatatt catgagaaaa
     4621 tggctcctat tttgtcaaat aaagaaattt ttggtgtaga tctttaccaa gcagagcttg
     4681 gcagaaaaat agaagaaatg ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa
     4741 cattacagga ttacttacaa gaacatggag gaaatttatc atggaaatga cttttagatg
     4801 gtacggatca caggaagaaa agattacctt agatgaaatc agacagattc ctgcggtcaa
     4861 agggattgta ggcacattga tggatatacc agtaggagag gtatggccta gggagaggat
     4921 caaagaacta aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt
     4981 caatgtgcat gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta
     5041 taagcagaca atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat
     5101 gcctgtattt gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac
     5161 gttagctttt atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcga
     5221 acattccagc aatgaatttg gattgccggg atgggaacct gagcgcttag ctcctatcaa
     5281 atccttgctt gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa attttgccta
     5341 ttttctccaa tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc
     5401 agatgatccg ccatactcga tttttggatt agctcgaatc gtaaagaatc gagaagattt
     5461 agattggttg tgtcgcatag tagacagccc ttataacgga atcactcttt gtacagggag
     5521 tattgcagaa gatccagaaa atgatgtata tgggatttta gcagaatttt gccaaagaga
     5581 tcgaatccca tttgcacatg ttcgaaatat taaattttta gctaatcgag atttctatga
     5641 ggcaccacat aaatccgaat acggttcatt agacatgtac cgcatactaa aaacaatgta
     5701 tgacaacgga tttaacggat acatccgtcc tgatcatgga agaatgatct ggaatgagac
     5761 aggaagacca ggatacggct tatatgatcg agcattaggt gctgtttatc taaatggatt
     5821 atgggaagca atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa aactgtaatt
     5881 ttattatgta aataagaagg atgttcaaat tcttgataca taaatccgga ggttgttaat
     5941 aagcattcgg atttatgtat cttatttggt ctaggaagta acttgatttt cttttttcga
     6001 ttgatccatg agtgcttctt cttttaattc aaagatatat tctgagatcc tcataatcac
     6061 ttttttccag tcttctggtg tgatgattcc ttttaaaata tagatttttt tgatatgaga
     6121 aggcaattgt ttcaaagggc agtcgctgat aacgatgttg cagttctcca taaaatcatt
     6181 ttgtattttg atcgattcat ttgaaaaaat ttgttctatc ttaacgatca gatctttact
     6241 gatataaaaa tcacgcgtat agttcacacc aatcacaatt cgcggctgtt ttttgtagcg
     6301 atcatacaaa tgtactaggt cttcaaccat ccattgcaca ttttcctttt ctaaaataac
     6361 tggtaacaag tgttgatttt gttctctgaa atccatataa aattgttcaa tggcggtttg
     6421 gacttctttc aatacgctat gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg
     6481 ttgattgaag ttaagaaatc cattataaag tttatttcgt gcgaatgcca atgagtaaag
     6541 aatcttttct ttttcaggta taaacaaatc aaatacagta caaagggact ccaccatttc
     6601 tcttgaataa agaacatgag gaacgtttgc tttttttaaa agtttgtatt gctccaagct
     6661 tgtttcttcc gattcgattt tcgtgataga aagtcgagct aaaatattca ctaagatgcg
     6721 ttgttcgcga caaattggca gattaggtcg caaaatatct acagagagat caataaaaaa
     6781 gtcaatatat tcatactctg tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa
     6841 attctcttta catattttta atagaaaata taatttatct atcataccgc tatttagtcg
     6901 gtcaagtact tcttttttaa aaaaagtatt catcataaaa gccactactt gcggatcttc
     6961 atcataaaat tctgtagcag tacttgaaaa gatgttccaa taaatatcta atgctgcata
     7021 ttgaatttgt acctcttttc cagaaaaaga ctttctgcca ttattagaaa aggagataga
     7081 caaaccgtac tatgctaaaa atgcattaat ctttttcatt ttcgtataca aataagaggc
     7141 actatagttt gtttgctgac tgaaactgat aatcgaattt gtctgacagc tgaacaaaga
     7201 agtcaagatc tgataactga ctgattcgtt gcagtatctt ccaaatagac gataatagca
     7261 gtaagccgga tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg
     7321 cagttctgtt agattgagac gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa
     7381 atttgattca gacata
//
LOCUS       DAZAFF010000116         7213 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000116 DAZAFF010000000
VERSION     DAZAFF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.119"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(173..898)
                     /locus_tag="ACG9BB_002590"
     CDS             complement(173..898)
                     /locus_tag="ACG9BB_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838461.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1052..1378)
                     /locus_tag="ACG9BB_002591"
     CDS             complement(1052..1378)
                     /locus_tag="ACG9BB_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838462.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1415..1780)
                     /locus_tag="ACG9BB_002592"
     CDS             complement(1415..1780)
                     /locus_tag="ACG9BB_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFN2838463.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1817..2032)
                     /locus_tag="ACG9BB_002593"
     CDS             complement(1817..2032)
                     /locus_tag="ACG9BB_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2838464.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2161..2496
                     /locus_tag="ACG9BB_002594"
     CDS             2161..2496
                     /locus_tag="ACG9BB_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2838465.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2572..4569)
                     /locus_tag="ACG9BB_002595"
     CDS             complement(2572..4569)
                     /locus_tag="ACG9BB_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838466.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(4667..4942)
                     /locus_tag="ACG9BB_002596"
     CDS             complement(4667..4942)
                     /locus_tag="ACG9BB_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2838467.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            5174..5908
                     /locus_tag="ACG9BB_002597"
     CDS             5174..5908
                     /locus_tag="ACG9BB_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2838468.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            5947..7089
                     /locus_tag="ACG9BB_002598"
     CDS             5947..7089
                     /locus_tag="ACG9BB_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2838469.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2426 a         1332 c         1031 g         2424 t
ORIGIN      
        1 atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct
       61 ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta
      121 cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa
      181 taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat
      241 aatctttttt cccattttca agtaaccgaa gtggaatatc tttaccaact ttctctgtca
      301 ctgcattact gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag
      361 aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagt ttccaagtag
      421 gtggtatagc tgtgtatgtt tctacaaatg aattaagttt ttcattaagt gacttctctt
      481 ttatatttcc atctaaaaac tctgtatata tggcttgagc tatactaaaa caaagctggc
      541 cttgttctaa taattttaca tcattagtta gtaaagctgt gttcacgtta tcaatcgctt
      601 caagaagagt atctttggtg tccctaacag ctttaggtgt gccttctgga atttccctac
      661 ttgatacctc gtccatttta tcttccataa accccttaaa atcatatctt tctcccaaat
      721 ccatagaagt ttttgagaaa gctttcattt ctgagtctat ttcttcaata atatttaaca
      781 tgtctgatac atattgttta ggctgattcg tttttaatct atatgtggta aatatctcaa
      841 ttcctccaat tatcaataaa attgccaaac aaatccataa taattttttc tttttcattt
      901 ttcctcctat acactgctgt tataacagtg actaatgtaa agattttccc agcaatcatc
      961 agactattat ttacagggaa tcaaaaaaac tccttagtaa gcttacacct ttaggagttc
     1021 ctgtttgaac ggtatgcata atacatttaa atcaatttaa cattaacacc tcagtatatg
     1081 gtatctctac atatacacgg tctttttttg gatagtactc gacatttata ttgatagcac
     1141 ttccattgtc taacgtagct ttagcccggt acgtgttatc accattatca tgagtgatcg
     1201 tcacatcatc acatgtccga tttaagttat actgttcttg taaaatctga gttaccaatg
     1261 gtacagatga cttctctagt ccagatgaac ccgtcattct tgaaaaaatt atacctataa
     1321 tagccaatac aacgatacct ataatgacaa acttcttatt catatttact ttattcaact
     1381 ttttatctcc tatctccaaa atgattttct ctcatcagta actcattgac atccccaata
     1441 gtacaccaaa gatggctcca gctacccctg ctatcatcaa aatcgtaccc atttgttcat
     1501 ctttctctcg atataagtaa cccataataa cagcaacagc accaaatacc acaggaataa
     1561 atgctaatga tactaccata gaaatccaac caataatcaa atacagcttc cctgtctctt
     1621 gtccatgtac gatttgttcc ttcatttcat taattgtctg ctcagtactt ttctttactg
     1681 tgtctgttcc ggttgtctca tctgtttctt gtaatgctcc gcaaaactgg caatacttac
     1741 tttcatttgt tatttcttta ccacattttc tacaatacat gtcttctcct cctaaaatca
     1801 ttcaattgct tattagctaa ctgtttactt tattaaatac atcagaaaac agctcattta
     1861 ttggtacact taatatctca gaaagcattt tcatttgata gggtttagga atcgttctcc
     1921 ctgtttgcca cgaacttaca cattgctgtg taacattcat ttttttggct aactcagttt
     1981 gggaaagtcc tttttctttt ctgtatcggc tgagattact tacttttttc atgattctcc
     2041 cctcctgttt tattatatac aacaatatga tgtatttcaa ctcataaaat atataaatac
     2101 ttcaaatgtc attacaaata caacaacttg ttgtactcta ctgagaaggg gggtgtatta
     2161 atgaaacaga agcaatatgc aaaggtcatt agagaaaagc gaaaagcact cgggtggaca
     2221 caaaaagaat tagctaaaaa aatcttctct acacaacagg cagtagcaag atgggagaat
     2281 tctgtaacag aacctaacct cgatagctta acagctcttt ctagagccct aggaacccct
     2341 gtaagccatt ttctagacaa tgttgacata gattatgagg aagagttttt ggctctctat
     2401 cgctctctta gtactgaaga tgcaattcga acaattgact atatgaagct attaaaaagg
     2461 caagaaaatg aacgcaatca gctattgaaa gattgaaaaa taaataaccc accgaacttt
     2521 aacggtgggt gtattgagat gaaataagat taagagctat ggataacctg tctagtttaa
     2581 tataattggg tctcttctat tccttactct ccgtccatca tctcttgtta cacttgtaaa
     2641 ttgataaata ggaactaatt caactcctga taatatcaat tcttttatta cttcatctat
     2701 ccctgcccct ttcgctagat atactaaaaa actactgctt gctttcacat tgtatagttt
     2761 cttaatatcc ttagaagctc gttcaagagt gaatccttgt ttaacaaagt agcctctgtt
     2821 ttctattaaa gcatttttca cgctgttatc cctgacattt cgtaggcgtt cacaaacagt
     2881 gttaataagt tctttttctg tcattagata tgtcccttgc cacgataagc cctgtaaaac
     2941 ttcatgtgct tcatctaaaa tcaattgttt ttccttgcta agttctgatc gggaattttg
     3001 aggaaatact cttttagcct ctttttcgtt aaaacatatc ttaacccatt cataagaaaa
     3061 atattgatat gtaaaatgaa atttttccag aagtggaagg atatcttcat tcaatttttg
     3121 aatcacagaa gttgcattaa aaacgtctaa tcgatagaca ttgcttctct tatgtttaaa
     3181 gtttcctaac gctttctctt cctttaacct atttaggtat tcgagcatct tcaaaggaac
     3241 atcttcatca tctaattttt ttatcaatcc tataaaagtc ataagtgata gtaacttatt
     3301 acatttatct atgtcataag ttttaccata gtaagaactc atttttttag ctaattgttc
     3361 ccccgacaaa atacaagtca cactttcctt actatcatca aaaaaattaa catcagaaag
     3421 caagacatca tagacacgtc gcatttcctc tagataattc cttattgtcc tgaaaagttt
     3481 gtctttcatt gattcaagag tgtcaaaaag tacttttgat tcctctttca attttcgaaa
     3541 atgtgtagga tacaagtcta ggttctctaa tagccaatta aatgtttcta cccgactgtc
     3601 tttctttaaa attagcatca caactccaat aaaattttca ctgatatcac aattactgga
     3661 atgacaataa aaaatggaat gcccagtttt actggtaaaa atactagcag agggattatg
     3721 atcgttgtga aacagacatc ttaagttttt acttgactta agtaatttat tcatatcaat
     3781 ggttaacaat ctttcataaa tctcattaaa agacatgtcg gaggaatcaa acaggcagtc
     3841 tttaaaccac tgtttcattt cttctttgtc gtcatttttt atcaacttaa caaaatcggt
     3901 ttcattgact agttcatact ctctccctga atcactttga caatttttag aacatgtcat
     3961 ggtaaaatca atcggaagct cagtaatatc taacatgttg tcttcattaa ttattatcac
     4021 tgcatctgac ttatatcctc caaaaaacat tctattcgaa gcatttacat tgtcatcaca
     4081 cccggggatg ttctttttca agtattcgta aacagcaacc attttaccat agtcagtaat
     4141 tgcttctttt aatagaaaga caatcttgta tttttctaga ttttcagagt accgaatact
     4201 tttttgtatc gcaaacgcag aattatgaac taaattgata gataaaacct tttctatgct
     4261 taagtatcta ctatcatctt tactcaaatc tactaccttc cccttctcat ctttaacagt
     4321 gttatcgaca tccagaataa ttactctggt ttccacaaaa ctctccttgt cattgccttt
     4381 attctttgtc ttgcttggaa taaatgattg tgatgaattt agtatctcta caaactcatc
     4441 tcttgtaact tctctatagt tatctaaaca cctatctctt acatcagttt tttctttttt
     4501 tgtaactggc ttctctaata atttttcttt atctacataa attggaatat taatcattga
     4561 tgtttccatt tgtgtttcct ccttataaaa ttctgttaaa gactaaaaca tacagataat
     4621 aaaaacaaac tctttattac tatatgtttt agtcttattt gtttactcaa cctttatacc
     4681 gttccatgaa actatcaata tcagattttt taaatctaat gaccgtatca atttttataa
     4741 caggtaaccc gtattgagaa acatattttc taatcgttcc tactgataca cctagatatt
     4801 cacttgcctg ctgatagtcc atatactcta gagtgactcc agaaataatg tcttctagca
     4861 taggctttag cactgtaatt atctctttca cttgttgttc aacttgtata gaaacaatgc
     4921 tatcaatgat ttgtagagac atatttcccc ctcctttcgt gattgcttga acaaattttc
     4981 taagaattat cttcgtattt tttgctcaaa ataaaagtat tttgttatta accgattaat
     5041 caaacaaaaa atgattacca gttcatcgac atacacataa tacctcgata aaaaaggggt
     5101 gtcaatagtt ttgtaaaatt tatttacaac accccttttt tgaggtatac taattttagg
     5161 aggtgtggtc aatatgataa aacgtccacc aataaattat ttagaacgaa aaaagatact
     5221 cggaacaaaa ataaaagcta taagaaagag taaaaaatta acacaaccag catttggtct
     5281 tatgattaac aacggtcaat taattgacaa aaagactatc tacgaatggg agaagggaac
     5341 ctatttacca ataccagagc gattatcccg aattgctgat ttgggaaata tgagtataga
     5401 ggagttagtt tgtggcaatg tagaagaata tattttggga attattcttt atagagatag
     5461 cattgtacta gatggtataa catttccaga taaaaacctg ttccagcatc ttcgtcaaca
     5521 gttcccacca gtacactcta atttagatac atggctggat agatattcca aattagaacc
     5581 agagatgcaa gaatttattg ctaacaagac ttgtaataaa gttaaaaatg aaaaaattag
     5641 tcttttcaat atcttaaaaa tagaagaatt attcatcaat gctattgtag aagaatttga
     5701 caataacatt cttttcctca ctagcagtat agaagaacta ctagaaagaa tggtagatga
     5761 atggctacct attcaattga aagacatgtc ttatcccgaa gaagctgtga gagaaattac
     5821 tgataatata aacaagctag aacaaacgat ttcatcaatt gggaaaaaat acactaagaa
     5881 aaaaatgaag ggaggtgata ccatctagtg gtaagtactc cttgtaaact aagaggagat
     5941 gatttcatgg caaaaatatc aaagtacaaa aagaaaaacg gtcaagtggc gtggatgtat
     6001 aaaggacaca ttgccaccga tccacgaact ggtgaaaaaa taaatactac gagaagaggt
     6061 tttagtagca aacaagaagc tcaaaaagat tatgacgagt acagacatca aatattatac
     6121 ggtgttaaaa agaaagcacc tgatatgaca tttgaagacc tgtataatga atggataact
     6181 catcagcgta ccagtgttaa agcttcaaca atcgcaatct cagtaagata cgcaaataat
     6241 caaatacttc cagcatttgg taagcttaaa ctatcgaata tctctgtccc atattgtcaa
     6301 aaagtggtag atgaatggca cagtaaatat gaaagttacg attatatgag aaaacagact
     6361 gcacaaattt tacgctacgg ggttgcaatg caatatattg acaataatcc tatggaaaaa
     6421 acacttcttc cacgaaaaaa agagtatgag aaaaatcgta aattttactc aaaagaggag
     6481 cttaataatt tactggatgc ttttaaagat tttggtaaca tgaaacaata cgccttcttc
     6541 agattattgg catacacagg tatgagaaag agtgaagtac tcgctttaca gtggaaagac
     6601 atagacacgt ttaataaaga actacatgtc aataaaactc ttgctgtaga tgaatttggt
     6661 aaagttataa ttcaatctcc gaaaacaaga gcttctagac gagttatctc tttagatact
     6721 gagactcttt ctatcctcaa caactggaaa ttacagcaaa aagaagagta tttaaaactt
     6781 ggatacaata ctagctctaa agagcaacat gtttttacaa cagttaaaaa cactctatac
     6841 attccaaaca ctgttaatga ttggttaaga tatattttga aaaaatataa cttgcctcgt
     6901 attacacctc atgggtttag acatacacat gctagtttat tattagaagc aggagaatct
     6961 gtcaaggtag ttcaacaacg attagggcat gaaaatagta aagtgacatt agacatctat
     7021 gcacatatta caaataatgc acctaagaaa acaggtcaag actttgctga tatgatggct
     7081 catcagtaaa agagctggtc aaaatctggt catttctagt atacaggcaa aacaaaaaag
     7141 ctagaaccct tatatatcaa gggttctagc tcctataaca ggtttaccca acactgcctt
     7201 ccaatgactc ttt
//
LOCUS       DAZAFF010000117         7195 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000117 DAZAFF010000000
VERSION     DAZAFF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.120"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..475)
                     /locus_tag="ACG9BB_002599"
     CDS             complement(251..475)
                     /locus_tag="ACG9BB_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838470.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(495..917)
                     /locus_tag="ACG9BB_002600"
     CDS             complement(495..917)
                     /locus_tag="ACG9BB_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFN2838471.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(1059..1769)
                     /locus_tag="ACG9BB_002601"
     CDS             complement(1059..1769)
                     /locus_tag="ACG9BB_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFN2838472.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(1787..2284)
                     /locus_tag="ACG9BB_002602"
     CDS             complement(1787..2284)
                     /locus_tag="ACG9BB_002602"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2838473.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(2321..2773)
                     /locus_tag="ACG9BB_002603"
     CDS             complement(2321..2773)
                     /locus_tag="ACG9BB_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN2838474.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(2908..3513)
                     /locus_tag="ACG9BB_002604"
     CDS             complement(2908..3513)
                     /locus_tag="ACG9BB_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFN2838475.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(3963..4583)
                     /locus_tag="ACG9BB_002605"
     CDS             complement(3963..4583)
                     /locus_tag="ACG9BB_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2838476.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(4585..5454)
                     /locus_tag="ACG9BB_002606"
     CDS             complement(4585..5454)
                     /locus_tag="ACG9BB_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFN2838477.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(5477..5884)
                     /locus_tag="ACG9BB_002607"
     CDS             complement(5477..5884)
                     /locus_tag="ACG9BB_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFN2838478.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(5903..7192)
                     /locus_tag="ACG9BB_002608"
     CDS             complement(5903..7192)
                     /locus_tag="ACG9BB_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFN2838479.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         1972 a         1521 c         1187 g         2515 t
ORIGIN      
        1 cttcatcttc catcccccta aatccgaaca ttataaataa agtccgttaa aatttcgatt
       61 tattaataaa acaacgtgtt ttatcatata ctaaaacaaa acataagtaa acaaaaaatt
      121 cggattttaa gcaaaatccg aacgttccat ctgattaaat ccctttatct caaagttcat
      181 gaacattcac tttggattac aagaatttct ttattgtgtt aataaagttt cttcattcta
      241 gtctcaaaaa tcaatagtgt gatttgattt taccatttcg tttctgttct tctttgatga
      301 attcttttgt atcgacagga ttcagattat ttagtccgcc aaaacctgta tcgaccattt
      361 ttccccagcc ttttgtcttc ttttttcctg atttgagatt ctttgctcga agcttctcac
      421 tatgttttgc tcgccgatac ttcaaataac cttctaatct tcccattaga tccactgttt
      481 ttcctccttt ttattcaatc agacgactca gcttttccta taacaaaata attgccttca
      541 gggtcagaaa aattgaaagt caagatgcca ttgttatctg tgatttctcc tggttcttcc
      601 atgttctcgt agatagtttc aaattcatcc gtaaaaaata ctaaagaagg cggtggtcct
      661 aatacctctg gtgaaaactt ctgcacaaat acttttggga atacgcctaa ttctaattct
      721 tttgagatgc tgagtacaat actcttaaat tcttctggca attcgattgt ttcagaaatt
      781 tctgcaccaa ttttttctac aaagaatcgg caaatcatct ctacgtcatt gacataaagc
      841 atcacacgtg ttttttgtat catcatttga cccttctttc tatttgattt tctttcctgt
      901 ctataaactg attccattat atcacgaaag cgcaaacacc ttgtgtttga tactattcaa
      961 gaaaaataaa gcaatagttt ctaggattgc tttcctccac aaactttttt tacaaaaaaa
     1021 aaacggcaat gaatgccgct cttcttattt cgattctttt ataattctgc ttctgattca
     1081 tctttggtta gtgtgtgtct aacactagtt acgccaaatg tttgatcgat tttttccttc
     1141 aacgcttcga ttgcctgtgc gccacgtgcc gcttgttcag cattgatgga ttcaatgacc
     1201 agcaccgctg cttgtgtttc ttccgtcagc atcgttcttt gggcttcacg ccagttgaga
     1261 cagcgacaga tcgctccttg ttcatcatag tagatgattt cttccggaag tgccggagca
     1321 tcggaatctg cacctaaagg caggaatgat tcgccacctt gggcttttcc taaatgcaga
     1381 tttcctgcga tcttttcgat atcttctccg ccgcaaggta cgccaaaaga caaggaaata
     1441 ctattataga tatctacaag cggattgatt gggaaaaatt cccgttcttg atttgcacgt
     1501 ttcagtaaag cttcaataga agaacgtgca ccttttttag tcttgaatgt ggtaaatgcc
     1561 tgacgccact gagcaatgac ttcgttttgg ctgaatgttt cttctgtaag gtaatttctt
     1621 gcttcttttg ctgcctcatt caataattga tgatacgttt cttcactttc ttttgttacg
     1681 tggttatcga taccatttac ggataagatt tcgatggttg cttcaggaaa tacttcccag
     1741 aaattggtat ccactattac ttttttcatc tgttcacacc tcttgattag aataattgct
     1801 tgatgaatag atagtctgtt tggggatcat ctcccatata aaaaacatgt tcgctgtatt
     1861 tgataaatcc ttttttacga taaaaagcga gcgcaacatc gttgtgttcc caaacaccga
     1921 gccaaatggc tgttttttgc ttttcttttg ctttttcaat agctgtatct agtaaaaatg
     1981 taccgatacc ttgatgttta aatttttttc gtacatagat tcgctcgatt tctaaaaaat
     2041 gatcagccat ttcctcagat tgactcgttc ctgtatttag tttcaggtaa cccgcaggct
     2101 cttgatcgat actcactagg aaaaaatcgg tttctttatc ttttagctct tctgtaagtt
     2161 tttctagtgc ataagcttgt gtcaagtaat cagctaaatg ctccggtgaa ttatcttttc
     2221 caaatgtatc tgtaaatgtt tcgatactaa tttgttgtag taagtctaca tctgaaggcg
     2281 tcatttttcg aatttcgtat ttattcacgg cttcttcctt tcaatagatc cttttattgc
     2341 cttttttgac aaaattccaa tcttcgctaa cattcttgca tactttattt aaaagatatt
     2401 ccaattgttt tgcttcttcg tcagaaagac catttaatgc gactgcattg gagtagttgt
     2461 tttctcgaat gatgaaagga tagatttctg ctcctttttt agtcggatag agtttcttga
     2521 tttttttatt ttctttatct tccaaacgtt cgatcatccc attggattct aatttcttga
     2581 ttgctcgggc agtcgttgta cgatctactt tgatcatttc tgctagtttt tcttggatga
     2641 ttccaggatt ttcgcaaata cgtactaaat aaagatactg tcctcttgtt aaatcgactt
     2701 ctttgaattc gatattgcta atagaatcta gcgctcgggc aatcattccg atgtctcgaa
     2761 ggatttcagc catttcatcc cacctttatt ttgtttgtaa gactagttca cctctttttg
     2821 ttgcaaatgc aactattttc aataaaaaaa tcaagttctt ttttaataac tagtaaagta
     2881 tattcttttc ttcttttacg aaaaagctca atcacaatag atctggtttt tgttttctaa
     2941 aataagtaac tgtttcttcg ttgttcgctt ttctgatagc tttggcaact cttctatact
     3001 aaaaaatccc atgttggaag tttcattatt ctcaataaac ttggcctctt caccgtggat
     3061 cgcacaagca aaaacaagtt tataatactg aaaaagctgt ggaatatctt tttgtttatt
     3121 cgtatcaaaa actgctcgca actgagtagt ttctactgtt aatcctgttt cttcatagac
     3181 ttctttacgc acattttctt caggagacaa tcctatttca gcaaatccac caggaagtga
     3241 ccattctttc gtccgtttat cttcgattag taggaccttg ccatcttttt tgatataggc
     3301 acgaacatct acttttggtg ttggatgtcc ttctccaatc gttgtgagcg cttttatttc
     3361 ttcgaaagtt tcttttccta tttcactgat cagttccaat gaaatcgtcc ggatctcctc
     3421 aaaacgctct ttatcaaata catcttttcc ataatataga ccagcttgag aaagtgcgag
     3481 tagtcttttg tattgagcca acttcttgtt cattctcttc ccttcttttt tagcaaatgt
     3541 atgactatgt aatcaagtat agaagactgg tttaattacg gcaaataaat ataatgaaat
     3601 ctgcaataaa taaaagaatg ttaaaaaact cgagaacaaa agacattcga cttctctggg
     3661 gctgtgaata aatggcgttc aaaagcttgc atctgcggta ataagcccca ccaaacgaaa
     3721 aatatgacga gctattcttg tctggctgtt cttattaatt gaggcaggac agctttttca
     3781 caacctcccc actaaacggt gttctcctac caactcctcg gtcacaagtc acaatattca
     3841 tcaaacatga gaagctgttt ttttgaaatt gctccttgtg actcggagct gaacgacagg
     3901 ctcacgacct ctcggtaaac agcgttcaaa agccaatttt tcgatattaa gccatttctt
     3961 tgttacttat tgtcattgat ttctttttct aatttatcca atagtggagt gtaatccact
     4021 tgagtatagt ctgttacatc aaattcatat agtgttccta actggaattt gtcatatttt
     4081 cggtcggcga tgatcttttt gaatgcttct ttcgtaatat gattggtccc tttcgttcga
     4141 tcttccaata cgtcaccata gtgataatga tccatgatat agctcaaatg acgatctgat
     4201 tcacgatcac gtttgtaccg acgttgccat aacacttcaa agtctgctgt taggcgaatc
     4261 gtgatcacct gatatccgta tctatctgct aattcttgga gtcgtgcttt ttgcttatca
     4321 ctaaaaggat actcggacac aatcactttc tttcctgctt ccatatacaa atcaagtgct
     4381 ttgtaataga aaggataaac tttcgtcctt tcttgttccg cccgttcttc caaattgtta
     4441 aacccgatgg attctgcata gtattccttg atttcatcga gcgtgatggc atacatatct
     4501 ggaaatcgtt cccgtagttt attcataaga tatgtttttc ctgtaccagg actaccagcc
     4561 agaagaatga aatacttttt catctcattt atttcctaag ttaattggac tttccaagtt
     4621 cataaacagt ccaatcttat gcttgacaat ttcttttact gcttcatttg ctggaatcac
     4681 attatggcga agagacttgt tgatttctgt ttcactaaag gtttctttag ctactttcgt
     4741 ccagttgact aaaagctccg ttccaacatt gaatttgcgg atcccgttat tgatcaattc
     4801 aggataatct tcttctttca cacctgttcc tccatggata actaggggga catcaactac
     4861 tgcatcgatt tctttcagca agggaatgtt tacttctgtt tttgatttga attgtccatg
     4921 attcgtaccg atagcaatag ctaatgcatc cacgcctgta gcttctacaa atttcactgc
     4981 gtcttccggt cttgtatata ccttgtcatt ttcagcgaca tggattcctt cttctgtacc
     5041 gccgattgtt ccaagttctc cttctacgga aacaccgtgc gcatgggcat attctactac
     5101 tgcttttgtc ttcaggatat tttctttgaa aggcagatgg gacccatcat acatgacaga
     5161 agtatatccg ctgtcgattg cttctttgat atcatcaaag tctcgggcgt ggtctaagtg
     5221 caagactgca tcaaccattt cttcttctgc aatagctgtc attacattga ctaacacatg
     5281 catgccgata tatttagcgg tatcgacact tgtttgaata atgatcggcg aacccatttc
     5341 ttttgctgcg cctagcatag caggaagcat ttctaggtta tgcgtattaa aagctccaac
     5401 tgtaaaattc aactcttctg ctgtttttgt tacttctttc aatgttgtat acatctttta
     5461 ttgccacctt atctttttat ttgtttcctt tgatcgttac ttgttttgca atttgtcgca
     5521 tctcatccat tttgcccatg tagtaatcga tcccttgatc atcaccagca cgagcagcat
     5581 ctgcacgctt ctggttatag aggctcatca gttttttata cccatctccg atcgtctgtt
     5641 tattttccaa actcttttct aaatattgga tggcttgatc gttcacatac aattcagaaa
     5701 atttaagtcc aacttgttca taaagtttcg ctaatttatg cttatcttgg gtagtggaaa
     5761 tctcttgttt caattgtgta atctctgatt gaatcttttc ggtcgcttct ggcgataggg
     5821 ctttttcttc ttttgccaga acttttttcg gttctttttc ttttttctta aaaaatccaa
     5881 acatcatttt ccctactttc aattaaaatg ctttcaaaac tgggcttaag atccaggcat
     5941 ataagagtgc tgcggctact gtatctacat cggttgctgt agcattgttg aatcccatcg
     6001 aattaaagat cgttactagc aaagcgggca ataatgtaat aaagaaacca tgagcaatac
     6061 cgccaatgat cgctcctcgg cgtccgccaa ctgcgttgcc aaatatccct gctgtccctc
     6121 cggcaaagaa attggttagc ataccaggta aaatcatcgc taagccgaac atgggcaaag
     6181 taaacatggc aataatcgta ccaatcgttg tcgtaatgaa acctaaaatg acagcatttg
     6241 ggctataagg aaagaaaact ggacaatcta aggcagggat cgcatcagga actaatttca
     6301 tagctatccc tcgaaatgcc ggcacgattt cacctaacag tagacgtaca ccagctagca
     6361 atacatacac acccactacg aattggatgg cttgtaaaaa ggcaaacatg atatagtttt
     6421 gatctcctga taattcagaa gcgaatggtt cgcctgcaaa taaaacagtg atgatatata
     6481 gcgggaccat cacgaccatg acagacaaat aagtatcttg cagaaattcg aaatgtgtcg
     6541 gcaacttgat atcttcgatc gactttttat tttcgccttt ctcaccaaaa agcttagcaa
     6601 caccggcttc aaataagtac ccgatcgtac aaaaatgacc taaagcaata tcatttgatc
     6661 cagtgatttt acgaatgatc ggttgcgcta ctgccggcat agcaatggcg aaacaagctc
     6721 cgataaaacc accgaccaag atcaatacga tccctcgcag accggcaaaa tagccaaaga
     6781 ctgtcgtcat cgttgccatc cataaaattg cttgaccagt taaaaaaatg tacttccatt
     6841 ttgtgaaacg agccaaaata atattgaata caaaaatggc taagaacgtc agtgcaatat
     6901 ctctacctaa acccaaatca ttcattgctt gaccattgat agattcaata gaagggatga
     6961 tcccttgcat atgaaatcct tcagtaaaga ttttcccgaa ataagttaaa cttccaacga
     7021 tgatgctcga accggcactc aacacttgaa atcctaggag tgtctttaac gtgccagaga
     7081 tgacttgtcc tgctgatttc ttctgcagta gtagacccaa catagcaatc aatgcaatgg
     7141 tgatcgatgc ctgagtcaaa atattttcga tgataaaatt gataacgccc atgtt
//
LOCUS       DAZAFF010000118         7013 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000118 DAZAFF010000000
VERSION     DAZAFF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.121"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..1215
                     /locus_tag="ACG9BB_002609"
     CDS             61..1215
                     /locus_tag="ACG9BB_002609"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFN2838480.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1237..1989)
                     /locus_tag="ACG9BB_002610"
     CDS             complement(1237..1989)
                     /locus_tag="ACG9BB_002610"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN2838481.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2353..5019)
                     /locus_tag="ACG9BB_002611"
     CDS             complement(2353..5019)
                     /locus_tag="ACG9BB_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFN2838482.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5360..6811
                     /gene="cls"
                     /locus_tag="ACG9BB_002612"
     CDS             5360..6811
                     /gene="cls"
                     /locus_tag="ACG9BB_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFN2838483.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2065 a         1450 c         1276 g         2222 t
ORIGIN      
        1 taatcttttt atgatacctt agtaaagtaa gttttttatc ataagtaaag aggtgttgaa
       61 atgaaaatag ggattgatcg tctttccttt tttattccta atttatattt agatatgaca
      121 gagttggcag aaagccgtgg ggatgatcct gcaaaatacc atatagggat tggtcaagac
      181 caaatggcag tcaatcgtgc aaatgaagac atcattacac taggagcaaa tgctgccagc
      241 aaaatcgtaa cagaaaaaga ccgtgagcta atcgacatgg tcatcgtcgg aacagaatcc
      301 gggattgatc attcaaaagc gagtgcggta attatccacc atttactgaa aatccaatct
      361 tttgctcgtt cttttgaagt aaaagaggct tgctacggtg gcaccgcagc tttgcacatg
      421 gcgaaagaat atgtcaaaaa tcatccagaa cgaaaagtac tagtcatagc aagtgatatt
      481 gctcgttaca gcttggcaag cggtggtgaa gtgacgcaag gtgtcggtgc tgttgcgatg
      541 atgatcactc aaaacccgcg tattttatcg attgaagacg acagcgtatt tctgacagaa
      601 gatatctatg atttctggcg tccagattat agcgaatttc ctgttgttga tggtccttta
      661 tctaattcta cgtatatcga atcattccaa aaagtttgga atcgacataa agaattgtcg
      721 ggtcgaggac tcgaagatta tcaggcgatt gctttccata ttccgtatac taagatggga
      781 aaaaaggcat tgcaaagcgt attagaccaa acagacgaag aaaatcagga acgtcttatg
      841 gctcgctatg aagaaagcat ccgttacagc cgacgaatcg gtaatcttta cactggttca
      901 ttatacctgg ggctaacttc tctactggaa aattcgaaat cactacagcc aggagatcgc
      961 atcggtctat tcagctacgg ttctggtgca gtaagtgaat tttttacagg ttatctggaa
     1021 gaaaactatc aagaatatct cttcgcacaa tctcatcaag agatgctaga ttctcgaaca
     1081 cggatcactg ttgacgaata tgaaacgatt ttcagtgaga cgcttcctga acacggagaa
     1141 tgtgcagaat atacttcaga tgtacctttt tcgattacaa agatcgaaaa tgatattcgt
     1201 tactacaaaa tataataaaa tcataaaaag aacccttcac gagtcgtgaa aggttctttt
     1261 tttatctgcg gaccaaagga gcagtctata ggtcagctta gtcgtactaa ggatttctcg
     1321 tttttcactg aatagtttgc gaatgtacct ggtcacaaca ggatcttttg cgtacgcgtc
     1381 atagtatagc ttcatcaatt cttcagtaat tgattcctgt gtaaaataag taaaatcttt
     1441 tttcttcact ttgaatcctt catcaattaa gtatgtttca aattgatcca ttacaactgg
     1501 cgatgtatta ggatcttctt ctatcattcc tagttgcttt tcgataggat aaaaaacatc
     1561 atcgattgat tccactactc tgattatcac gcaccatttg cttgactttt tcatcaattt
     1621 ttctaattga ataagatgta ccaatgctgg atttgctgct gtaaaaacca tttcatatgt
     1681 ctcatttgat tggataaatt cttctaacga ctgtttgata aaggaaacat ttggcaactc
     1741 atttttttct gcttcttttt ttgcattttc tagcattcct gaagaaaaat cgatcaaatc
     1801 atagcgatta atttcttttg ctaaggaaag aaatctgcca gatcctccag caacgtctgc
     1861 tgctgtttca gcaggcagga ctttctccgt tttcagataa ttttttacat cttcagcaat
     1921 agttacttgc gattcttgct ggatttcata atattcttca gcaaattcat cccattcttt
     1981 ttcattcatt tgatttcctc taattttttg ttatatgttt tcacttttcg gaataaatca
     2041 ttctcctctt ttatctattc tatctctttt gatgaataat aaaatgattg gctttccgtc
     2101 tatcataaat aaaaaaaggg actgtgacca aagtcgtgtc aaagtccctc tttccgaata
     2161 aacagtgtcc aatcagatga ccctcagtat tagctcaaaa tgctgatttt acaacttctt
     2221 ccttaaacgg tgttctcctg ccaactcttc ggtcacaagt cacaatattc aacaaacatg
     2281 agaagctgtt tgttgaaatt gcaccttgtg actcagaatt aaacggcagg ctcacaacct
     2341 cgttgattcg ttttatttat ttccttgtac tcgtacaatg atcaatttag tgatttccat
     2401 caaaacaatt gtcacaagtg cgattcccat acatgtaaag acttctgtca atccaaatgc
     2461 tgatgggata gagaagactt ctttcagtcc tgggatcatt gttaagcagt aaagcgcgct
     2521 acatacaact acagctagta agacacttct gttagagaag aaccctgctt ggatagctgt
     2581 ttgtgtgttt gaacgtgctg ggaatgtttg caatgtccga caccaaatca atgttgaaaa
     2641 tgacatggct acaccgaatt caggtgaatg ctgcatgcca agccattgag caaagataac
     2701 agcaatccca atcagcacac cacgatacgt tacagaaatc aatgttttgt ccgtaaagat
     2761 tcctgaattt ggatctcttg gcgggcggtt catcgtagca ggttcagctt tttctgtacc
     2821 taatgcaatt gctggcagtg aatcattgac taaattgatg aacaacagtt gtaatgctgt
     2881 aaatggattg gaccagtcag caactaaggc aaagatgatc gcaatgatcg cacctaaatt
     2941 tcctgcaaat agataagaaa tagctttctt gatattatca aacacattac gtcctacttc
     3001 tactgcgctg acaatagaag caaagttatc atccgtcaat accatcgctg ctgcatcttt
     3061 tgccacatct gtacccgttc ccatagcaat tccgatgtct gcttgtttca atgctggtgc
     3121 gtcatttaca ccatcacctg tcatagctga gatatgacct ttattttgcc aagcacgaac
     3181 gatacgaatt ttattctcag gtgaaacacg tgcataaaca gtgacttttt ctaaaacttc
     3241 gtttagttga tcttctgtca attcatctag ttccgtcccg gtcaaagcca ggtttccttc
     3301 ttcaaaaatc ccgatttgtt ttgcgatagc aacagctgtt gttttgtggt caccagtaat
     3361 catgattgtt ttgattcctg cacttcttgc ttctgttacc gcctgagtca cttcagcacg
     3421 tggaggatcg atcatagcaa gcaaaccaac aaggatcaaa tcgtcttcat cttcaaatga
     3481 taaatcaaaa ttatcaagtg gacggtaagc aaaagctagt acacgtaatg cgcgtgttga
     3541 aaaagcttca ttttgctctt ggaatttttt catcaattca tccgtcatcg gaacgacttc
     3601 accatttagc aatacttttt tactgcgttg gaagacgata tctggtccgc ctttaacaaa
     3661 caagtaattt ttgttatcaa ttgtgtgtcc agtagacatt aattttcgat ctgagtcaaa
     3721 aggaagttct gcttgtcttg ggtagttgtt tcggatttcc tgataaggct ggtttaattt
     3781 ttcactaaag tcgataaacg ctacttccgt agggtcgccc aatttagtac cgtcttcact
     3841 gatcgatgca tcattcgcta aaacactgat ttcgatcaag cgacgttcgt cagctgtcca
     3901 ttttgaaggg tcatttgtga aatccccagt atcgccatta cctaaaaagt agtcaacaac
     3961 tgtcattttg ttttgtgtca atgtaccagt cttatctgta cagatgatac ttgtcgaacc
     4021 aagtgtttca actgctggaa gcttccgaat aatggcatga cgttttgcca ttttattcgt
     4081 tcccaaagat aacacgattg ttacgataga ctgtaatgct tctggaatag cagctacggc
     4141 tacggctacg gcaaacatca ctgcgctgac gatatctgca gtcatatctc ctgtaccttc
     4201 tccaagataa atacgaaaca gttggatacc taaaatcaat aaagacaatg ccaaaatagc
     4261 caaacttagt tttttactga atttatccag acctcgttgt aacggagtga cttgctcagt
     4321 tgttgattcc aaaaggccag caacttgtcc gatctccgta ttattcccag ttgaagtcac
     4381 taagaattta ccgcgtccgt aagttacgat cgttccactg aataccatat ttgtacgatc
     4441 tccgattggt accgtacttg tgatatccgt tacttctttt tctgcagaag tcgactctcc
     4501 tgtcagcata ccttcatcta cttttaatga tcccgcatct agtaaacgtc catcagcagg
     4561 tacataatcg cctgcgtcta acaacacgat atctcctacc acaagttcgt ttgccggaat
     4621 cgttttttcc ttaccatcac gaatcactcg agcatcgggc gctgaaaggt ttttcaacgc
     4681 ttctaaagaa ccttctgctt tttttgtttg gacaacactg attatagaat tcaacatcaa
     4741 tactgcaaaa ataactaaga attcgatgta gtcacccatg accatctgaa tcagtgcgac
     4801 aatcagcaat acgataacca ttgcatcttt gaaagtttct aagaacaatt tccaagttgg
     4861 cttcttcggc ttttctttta gctggttcaa gccatctctt tctaattttt ccttcgcttg
     4921 cgtggtgctc aatccatcgg ttgagctctg cgtttgctgc atcaacgagt cgatcgtttc
     4981 tttatagacc gcttgttgtg cttgttcctt gttttccaaa tttcatcccc cattttttga
     5041 tcttggccct agtcctgtct gaaaaaaaag agacctaaga caaatacgca tttgtcttag
     5101 gtctcaccat ttcatacaat accagaaaac catttggtta acttactgtt gatattatcg
     5161 caacaaacgt tgccggttac tcccctaagg agatattcaa ctgtactaat atcctacctt
     5221 attaacctac aaaaagcaac tactttagcc cgatttacct acattttcat gaaaggagtg
     5281 aaaagtatat tcaccttggt aggcttcttt cttctatcat gctatactaa tgttattgta
     5341 tgtaaggagg aacatttttg tggtatctag tattataacc gccctttatc tattaaatgc
     5401 acttattgct ttggtggcta ttttgatcaa accccgagat gtagcagcca tttgggcatg
     5461 gctcttagta ttgtttgccc ttcctggggt gggctttgta ttatatttat ttttcggacg
     5521 tggattaacg gataagaaaa aattctatct ccgacaaagt gacttgaaag aattagaaaa
     5581 ctttcagtct tttagaggag ataccattga acattacgat cctgacatgg gcgataaaga
     5641 caaacagcaa tttgttgact tcttctcttc attaaatcgt atgccgctga caagaatgaa
     5701 ttctgtcact cttctcacag acggacaaga gaaattggat tcactgcttc aagatctaaa
     5761 aaaagccaaa cattcgatcc atatcgaata ttacgcattt gtgacagata atatcggcca
     5821 gcaagtctta catgttttag aagaaaaagc cgcagaaggc gtggaagttc gaatattata
     5881 tgatgcattt ggctctcatg gcacaaaagc aaaagatttc aatcgtctaa tcaaaaatgg
     5941 tggacatgtc catacatttg ttacctcaca aagggcatta cttcgtttcc gattgaatta
     6001 ccatgatcac cgaaaaatcg ttgtgatcga tggaaagatt agttataccg gtggtttcaa
     6061 tattgccaat caatatgtaa atacaacaaa aaaattcggc tattggcgcg atacgcatat
     6121 acggattttc ggtgccgctt cttctttgct ccagcttcgc ttcttaacag actggaacgt
     6181 ctcggtacct gaagaaaaaa aggtcggcta tcatttgaat tatttcttta aaaaagcaga
     6241 tcgagatgaa tctaagcttg ctgatacatc catccagctt gtttcaagcg gaccgaataa
     6301 cgaaagggaa caaatcaagc tttcatttat caaattgatt acttctgcta aaaaacgtgt
     6361 ttggatacag acaccttacc ttgttcctga tgaaagtgtc attgctgctt taaaaatcgc
     6421 aactgcctct ggtgtagatg tgaaaatcat gattcccaac aaaccggatc atccttttat
     6481 ttatcgagca acacaatatt atgctcggca gctgatcaag gaaaatgtac aaatccttgt
     6541 ctatgagaac ggcttcctcc atgcaaaaac attgataatg gatgatgaaa tctgcatggt
     6601 aggttcagca aatcaagata ttcgtagcta ccgattgaat tttgaaacaa gtgctgtcat
     6661 ttacgatcct gagtttttag aagaacttgc tactcagttc aaagaagatg agacacattg
     6721 ttcatccatg acaactgaaa cagtcaagga aatgtctaac tggctattat tcaagcaaca
     6781 aatttctcga ttattttctc caatcctata atttttttat tcaagtagag aaaaatatac
     6841 taagattaaa gggactgtga caaaaatctt gtcacagtcc cttcttccga acaaacggtg
     6901 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgtttt
     6961 ttgaacttgt tccttgtgac tcggagctgg acggcaggct cacgacctct ttc
//
LOCUS       DAZAFF010000119         6877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000119 DAZAFF010000000
VERSION     DAZAFF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.122"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(128..331)
                     /locus_tag="ACG9BB_002613"
                     /pseudo
     CDS             complement(128..331)
                     /locus_tag="ACG9BB_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            327..494
                     /locus_tag="ACG9BB_002614"
     CDS             327..494
                     /locus_tag="ACG9BB_002614"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838484.1"
                     /translation="MENTFELYNKTTVDKLIPILVPTDLNGHDVLLRFNGFLGEDYRY
                     LKISDPQPLTE"
     gene            complement(537..1112)
                     /locus_tag="ACG9BB_002615"
     CDS             complement(537..1112)
                     /locus_tag="ACG9BB_002615"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFN2838485.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1267..1793)
                     /locus_tag="ACG9BB_002616"
                     /pseudo
     CDS             complement(1267..1793)
                     /locus_tag="ACG9BB_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2250..3761
                     /gene="gadC"
                     /locus_tag="ACG9BB_002617"
     CDS             2250..3761
                     /gene="gadC"
                     /locus_tag="ACG9BB_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFN2838486.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3774..5174
                     /locus_tag="ACG9BB_002618"
     CDS             3774..5174
                     /locus_tag="ACG9BB_002618"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFN2838487.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5339..6774)
                     /locus_tag="ACG9BB_002619"
                     /pseudo
     CDS             complement(5339..6774)
                     /locus_tag="ACG9BB_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2140 a         1179 c         1230 g         2328 t
ORIGIN      
        1 tcaataaaaa tatattacaa taataatcgt aatagctaat gaaacaattt ctcaatcttt
       61 ttaccactcc aaacagggag cgtcaataat tttgtgtaaa taactcgtcc ttctgcaaaa
      121 taatgggtta ctcagtaaac attgaagcta atgtatccgt cacttgttgg aagcctttat
      181 ggcttctatt taagaatttt tgattgtatg tatcaaagat ggatactagg aaacgctcta
      241 gtgattcttc attttgaaac tgctcttttc tgcggctgta tttcttgatt tgtttattga
      301 aagattcgat tgtagaacca gaaggaatgg agaacacttt tgaactttac aacaaaacta
      361 ctgttgataa attaattcca attttagttc caactgattt aaatggtcat gatgttttac
      421 ttcgttttaa cggattttta ggtgaggatt atagatacct aaaaatttct gatcctcaac
      481 ctttaacaga ataataaaaa acgtgtaatc aattatttga ttacacgttt tttatttcaa
      541 tcttcgttgt agtagtggtt gcgacatacc gaaaggtaat cttcattacc tcctactaca
      601 atctgttcac ctgaataaac tggaatacca tcaacaatac gagcattcat tacagccttt
      661 ttattgcaaa aatggcaaat agttttaatt tcctcaattt tatcagcaaa tgtcaataat
      721 gcttcggatc ccttgaagag attattttga aaatcattct tcaatccata gcaaattact
      781 ggtacttcct cattcattac gataagtgat agatcaataa tattctctct acttaaaaat
      841 tgagactcat cgaccaaaat acaatcaact tttccgtact gatcaataat ctccactaat
      901 tctccgtaca catccatttc agaagtaatt gcaatagctg gtttcgaaac acctatacga
      961 ctctcaactt ttccaaatcc tgatcgagta tctattgcgc ttgtcattat gataactctc
     1021 ttcccttgtt cttcataatt atgagccact ttcagtagct caatactctt tccactattc
     1081 attgttccat atctgtaaaa taattgtgcc attaaattta ttcccttctt ttacaattca
     1141 attattcctt aattccatct gcttttatgc agctgctcgt ttattccttg atatgtctat
     1201 aacatctact atccctaaaa ccaatttata agccattaga gatggtattc ctgaacatct
     1261 ctcatattgt tttttgagtg gtatatcatc tgaaaaactg aaggaatcat gaaaatagcg
     1321 acatacttat catttagagt tctaacttta aatgcattta cttcttcagt cagcacttta
     1381 gtcatgttgg acatggtttg gggcgtgtag tgatgcccat acattttctc aatcaagtct
     1441 gcgatttccg acatggtaat accttttcgg aagaggtgaa tgacggtctc ctctaatgtg
     1501 tcattcgtcc gtctataagc aggaacagtc tgttgcttga actcgccatt gcggtcgcgc
     1561 ggaatctgga gatgaagttc cccatactcg gtcttgaccg tacggtcata ggagccgtta
     1621 cgagaattac ccgtgttaaa accaatgcga tcgtactttt cgtaatcgag gaaagccgtt
     1681 aactccgttt tgagaagcgt attgacagct ttttccaaat ggacacggaa taatccattc
     1741 aaatcgcctt tgttcgctag agtctttagg atttctgtag taaaatcgtt catatggaag
     1801 tcctcttttc tgtgaatgtg ttgtggtaac tctattctac agaagggact tccttttttc
     1861 tatttacaca aaatatttta cactctcaac aaaagtatct tcgattaata cttgctcaag
     1921 ttatgtgcag ctacgaaaaa gtaggtaccg aggtactttc tttttcgcag ctttttttgg
     1981 acttatgaga agtacattcg ctggaagaat acgatataaa acgagctttt tcactaaaga
     2041 tattaataga gtgaaagaaa caagcttttg ttgtcagttt tttcactttt ctaaaaatga
     2101 aaataaaaaa ttctggaagt ggagaagaat tttgcttgct atactttgtc ttgtagaaaa
     2161 gaggtagaca aaataatgca agctcaaata agcgattttc cataaccttg gtaggaatga
     2221 caaattaaat aggaaaggga agtaataata tgaataaaaa aaagttatct ctattcggtt
     2281 tttttgccct gacagcttcc atggttttga ctgtttatga gtatccgacc tttgcaacat
     2341 cacaattaca tttggttttc tttcttcttt tgggaggtat tttatggttt ctaccagttg
     2401 ctttatgtgc tgcagaaatg gcaacagttg aaggatggaa agatggtggg attttcagtt
     2461 gggttagtca aactttagga gaacgttttg gttttgcagc tatttttttc caatggtttc
     2521 aaattacagt aggttttatt acaatgatct acttcatact aggagcgctg tcgtatgtaa
     2581 ttaattttcc agagcttaat acagatccat ttatcaagtt tattggtgta ttagtcattt
     2641 tctgggtact gactttttca cagttgggcg ggaccaatcg taccgctaaa attgctaaag
     2701 caggatttgt cattggaatt gttattcctt cgatcattct ctttggttta gcagcagcct
     2761 actttatcgg cggtaatccg attcaaatgg ctattaatgc gaaagctttt attcctgact
     2821 tttctcagct ttcaacttta gtagtatttg tatcttttat attggcttat atgggcgtag
     2881 aagcttcagc atcacatatt aatgagctgg aaaatcccca aagaaactac ccattggcaa
     2941 tgtttttact agtgattttg gccatcgcct tagatgcaat cggcggtttc tcagtagcag
     3001 cggttattcc acaacatgac ttgtctctca gtgctggtgt aatccaaaca tttgaaacgc
     3061 ttattttaca ttttaacagt catttacatt ggttggtaaa agtgattgct ctcatgattg
     3121 cctgtggcgt tatgggtgaa gttagttctt gggtagttgg tccatcacga gggatgtttg
     3181 cagctgctca gcgaggtctg ttaccagttt tcttccggaa aactaataaa caagacgttc
     3241 cagttccgtt gattatgata caaggaatta tcgttactat ttgggcagca gtcttaactt
     3301 ttggtggagg aggcaacaat ttatcattct tagtagcaat ctccttaact gttgtcattt
     3361 atctcgtagg atatttacta tttttcctag gttactttgt attagttttt aagaaaaaag
     3421 acttaaaacg aacttacaat gtaccaggaa aaactgttgg caaaacgatt atcggtggaa
     3481 ttggttttat tttatcagtg ttcgcattgc ttatctcttt tgtaccacca gcttcaattg
     3541 cagctagtga gcaccgaaca taccaaatca tactacttat tagctttgta gtgactgttc
     3601 ttttaccttt tatcatttat gaagtacatg ataaaagtgc tcacacaact attgaggaac
     3661 ctactcacgt gaaagctgaa gacgttaatc ccgctgttta tccagctgct agaggagagc
     3721 accacgttac aaaaaatgat gaacatattt taaaacatta ggaggaattc attatgttat
     3781 acggaaaaga taatcaagaa gaaaaaaact atttggaacc aatttttggc tctgcaagtg
     3841 aggatgttga cttgcctaaa tataagttaa acaaagaatc cattgaacca cgaattgctt
     3901 atcaattagt acaagacgag atgttggatg aaggaaatgc gcgattaaac ttagctactt
     3961 tttgtcaaac gtatatggaa cctgaagcag tgaaattgat gacccaaacg ttagaaaaaa
     4021 atgcaattga taaatcagaa tacccacgaa caacggaaat tgaaaaccgc tgtgtaaata
     4081 tgattgctga tttatggcat gctccaaata atgaaaaatt catgggaact tcaacgatcg
     4141 gctcttcaga agcctgcatg ctgggtggta tggccatgaa atttgcttgg cgtaaacgtg
     4201 ctgaaaaatt aggtcttgat attcaagcaa aaaaacctaa cctggtgatc tcttctggtt
     4261 accaagtttg ttgggaaaaa ttctgtgtat attgggatgt ggaactgaga gaagtcccaa
     4321 tggatgaaaa acatatgtca attaatctag atactgtcat ggattatgtg gatgagtaca
     4381 caattggtat tgtaggtatt atgggtatta cttacactgg tcgttatgat gatatcaagg
     4441 gtctgaatga tttagttgaa gctcacaata aacaaactga ctataaagta tacattcatg
     4501 ttgacgctgc atcgggtggc ttttatgcac catttactga acctgatcta gtttgggatt
     4561 ttcaattgaa aaatgttatc tcaattaatt cttcaggtca caaatatggt ttggtatatc
     4621 caggtgtggg ttgggtctta tggcgtgacc aacaatactt accagaagaa ttagtattta
     4681 aagttagtta cttaggtgga gaaatgccaa ctatggctat caacttctct catagtgcag
     4741 cacaactaat tgggcaatac tacaactttg tacgctatgg ctttgatggt tatcgtgata
     4801 ttcaccaaag aactcatgat gttgctgttt atttagccaa agagatcgaa aaaactggta
     4861 tttttgaaat cattaatgat ggatcagaat tgccagttgt gtgctataag ctgaaagaag
     4921 atcccaatcg cgaatggaca ctatatgatt tatctgatcg tctgttaatg aagggatggc
     4981 aagtcccagc ctacccatta cctaaagact tggatcaatt aattattcaa cgcttagttg
     5041 ttcgagcaga ctttggaatg aacatggctg gtgattatgt acaagatatg aaccaagcaa
     5101 ttgaagagtt gaataaagct catattgttt atcataaaaa acaggatgtg aaaacgtacg
     5161 gctttactca ctaaaattct tcagagaaat ataaaagaag ttaattaact aataaaagga
     5221 gttgtgatta ccacacagct ccttaaatct atttgaataa atatatgata ttcatttgtt
     5281 acactctcaa tcttagctgt tgttctattc atcattactc ttattgtaag tagttcagtc
     5341 aaatttgctt gttcaattga tcgataacag tatgaattgt ttgaaaagta atcggatata
     5401 taattgtttt ctcactacct aagccttctt ggtataaaga actatcgcaa agtataatcc
     5461 gctgagtaca aaagaaagtg tcgtctacta tttctctaag ttgtttacaa atgatattac
     5521 taatagaaaa gtgttctccc aaaatgtctt ctaactgttg tcgatagtat aaatacctaa
     5581 agtctgacgg agctactatg aacacttcaa ttggagattt ccgtttatgc tcatttaaaa
     5641 gacttaaact aatggttagt ttacgaatag aattctcatt ccaacgcaga aataaattgt
     5701 tttgttttcg ccattgttca agaatttcat gaacttctaa gtacaaagcg cgttgctctt
     5761 catttaacaa gtaagtctta tcaggttgaa atatctgaat atcagcccaa agtgagcgga
     5821 taaacgggag aagtgcttta tcaaaaagta tctgattttc ttcagatata ttcagagata
     5881 agataagttt ttgactgagc tcaatcacca aagggttatc tgctacatgg ttttgataaa
     5941 caacgttcaa gtctttttgt aacaattcaa agttatcgca tagataattt ctagagttga
     6001 atagaaagta gatataaagg cattcatctt cgtcatgaca taaagagaaa gaaagttgct
     6061 ctaatccttt ctgtattagc tgaaacaagg gagtatgtcg ggcaactctt ttttcttcct
     6121 gactaaaata tattgggtta gtcttagatc tctgcaaacc gatagcgata cctaataaaa
     6181 taaattgttt ttcaactgta gggtaacttc ttgaaaaaaa tgtttttcta cataatcaat
     6241 caattgttga ccgctttgaa taattgattt atttagctgg atcaactctg tccgatttgt
     6301 ctcatagatt atcgttagta acaaacttcg ataatccttt tcaggaattt caaaatggcc
     6361 atcagcattt aagtactgca tttgtttaaa aaaattaatc aactcatttc gtaagttgta
     6421 aactttgctt aacgaaatga aggtagtatc agctatatta ctccaattgt gttcccctga
     6481 taaataatgt cctagtacat tgaaaaagtc agagcgctga tagatgagtt gagtgagtgc
     6541 caacctcgaa aacgaagagt cgaatgcgac tgtaaagcta cttctagatt tctccactga
     6601 aataatttgc tgatggagta tgtcggtaat gcattccaag tcgttagtga cagtgagtgg
     6661 gcaaacgttc aattgatctg ctatttcgcg aatctgaatg gtcccttttt cgtgtaattg
     6721 ctcacaaatg taaacttgac gaaaaatact tttctccaga taggtttcca acatagtact
     6781 cctcctagtc attttataca taactattat aactctacca atcggaatag tcctgtaata
     6841 tattgaatca aaaatataaa ttataaaata attagat
//
LOCUS       DAZAFF010000120         6679 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000120 DAZAFF010000000
VERSION     DAZAFF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.124"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1273..1938)
                     /locus_tag="ACG9BB_002620"
     CDS             complement(1273..1938)
                     /locus_tag="ACG9BB_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2838488.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="ACG9BB_002621"
     CDS             2064..4100
                     /locus_tag="ACG9BB_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFN2838489.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="ACG9BB_002622"
     CDS             4104..4742
                     /locus_tag="ACG9BB_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2838490.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ACG9BB_002623"
     CDS             4770..5198
                     /locus_tag="ACG9BB_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838491.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="ACG9BB_002624"
     CDS             5655..6566
                     /locus_tag="ACG9BB_002624"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN2838492.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2297 a         1010 c         1225 g         2147 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta
     6661 cttgatttag ttcttttta
//
LOCUS       DAZAFF010000121         6644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000121 DAZAFF010000000
VERSION     DAZAFF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.125"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG9BB_002625"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG9BB_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG9BB_002626"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG9BB_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFN2838493.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG9BB_002627"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG9BB_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG9BB_002628"
     CDS             complement(2143..2373)
                     /locus_tag="ACG9BB_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFN2838494.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG9BB_002629"
     CDS             complement(2603..3421)
                     /locus_tag="ACG9BB_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFN2838495.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG9BB_002630"
     CDS             3582..4271
                     /locus_tag="ACG9BB_002630"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFN2838496.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG9BB_002631"
     CDS             4285..5787
                     /locus_tag="ACG9BB_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFN2838497.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG9BB_002632"
     CDS             5800..6276
                     /locus_tag="ACG9BB_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFN2838498.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG9BB_002633"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG9BB_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZAFF010000122         6629 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000122 DAZAFF010000000
VERSION     DAZAFF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.126"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..546
                     /locus_tag="ACG9BB_002634"
     CDS             <1..546
                     /locus_tag="ACG9BB_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN2838499.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
     gene            complement(716..1678)
                     /locus_tag="ACG9BB_002635"
     CDS             complement(716..1678)
                     /locus_tag="ACG9BB_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2838500.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1680..3119)
                     /locus_tag="ACG9BB_002636"
     CDS             complement(1680..3119)
                     /locus_tag="ACG9BB_002636"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFN2838501.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            3304..5250
                     /locus_tag="ACG9BB_002637"
     CDS             3304..5250
                     /locus_tag="ACG9BB_002637"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFN2838502.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5514..6386
                     /gene="scrK"
                     /locus_tag="ACG9BB_002638"
     CDS             5514..6386
                     /gene="scrK"
                     /locus_tag="ACG9BB_002638"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFN2838503.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         2120 a         1141 c         1180 g         2188 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtccc gtccaatttc gtaaaacaac cgtaacagtt
      541 gtgtaaaaat atcattttag agcatgaaaa aaggagtgga tttctccact ccaaaaaagt
      601 ctaacttttg ggggtcacta caagttgaca actccttttt gtttacaaat caattggata
      661 tctacaaatt gtattttcta acgaatttta tagacattaa aaaattaatt taacgttaaa
      721 tacttcctcc agaaagtatt gagattggta aaatataatc cttatttgtc ttttcgcctt
      781 taattttttt cagaagaata tcaacaataa gacttgcaat ttcatcaatt ggttgtctta
      841 ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt accatcgtat ccaataattt
      901 ttaaatcttc tgggatatta agtttaagtt gatgagcaat tttcatagtg agaatggctg
      961 ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt tataatagtt gactttattt
     1021 ccatttcacg acggatagta gacagattat taggaatttt gaaaattttt ccatttggat
     1081 tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga atccgtgtta tcatttcctg
     1141 taatcataat ggtattttga caaccatttt tttgtaaagt cttagctgca agttttcccc
     1201 cttcaaagtt atctgaagaa acaatggaaa cattaggagc taaatttcta tcaaaagcaa
     1261 caataggtgc tctcacacgc tcataatcag caattccaag attatggctt gatgagatta
     1321 tgccatcaac ttggttggct tcaagcattt caatgtattc tctctcttta gcaggatcat
     1381 tttgactatt gcaaataatc gttttatatc catgtttaaa taactctatt tcgagatact
     1441 caatgagttc agagtaaaag atattactaa tattaggaaa aataagacca ataagctgag
     1501 gtgactttcc ttgaagtcca cgtgctaaat tatttggttt ataaccaaga gttttcatag
     1561 cttcatgaac tttttgtttt gttttttctg ataaatatcc cttattatta atgacgcgtg
     1621 aaacggttgt tgggctaact ccagctaatt cagcgacatc agttaatttt gctaccattt
     1681 taatatttta attcaaagta ttttccagta ggttcgcctg atttgatttg aataccattt
     1741 tggtgatttt cagggaatac acgactagta aacacttttt ctcctttatt aataaaaatc
     1801 tcaatgattg agttatctac aaaaatgtta gcagttgttt ctttagtatc aattgagcat
     1861 tcacgaatcg taccaaagtc agtggcatat tgaatgcctg ctttgctgcg atctaaaata
     1921 atttttcctt ctttggtatc tacaatcaag ctaagtccat ttcctttact atcagagaaa
     1981 agaagtaact ccgatttctg atttggagga aaagttagtt caagctcata agtattattt
     2041 gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta atgatgtaat agcttctact
     2101 ggatattggt aaagtttacc atcttttatt gaaagttctt ttacaagact cattgctccc
     2161 tgataatcat acttatcagt tggataatcg atatcaggta gtccaatcca actgacaatc
     2221 agagcacgac catcaggagt attgaatcct tgagtcgcat acgcttcaaa tccataatca
     2281 agattttgaa tttctgaagc gcccacaagt tttacttttt ctatgtcaag cgattgacag
     2341 atcttaaaag tattcggata gatattccca taatttagct cagatttatc caacccttga
     2401 ggacaataaa tgagaacagg ttttttgtct acaaatacta gatttggaca ctcaatcatg
     2461 tattctgaac cagtgccgcc aaaatcaagg ttgccaactt cctcccatgt ttccacgtta
     2521 ttgtcatccg ctttgtaaag tttgataaag cctgattttt ctaaattttg tgctccaaca
     2581 atcgcataca attgtccttc ataattaaac atttgtggat cacgaaaatg atcagttaca
     2641 tcagcaggtt tatgaataag aacattctca cctttcgtaa tgttgtagtc tttatccatc
     2701 catgctccga tttgaagtgt atcacgttcc caattttcat ttcgaacgtt tcctgtatag
     2761 agtaagaata gattcccctc tatttcatat gcagaaccgg aataagcgcc gtggctatct
     2821 cgttttgtgt ctggaagaag ttctgctcct gttacatgaa aatgaactaa atcatctgat
     2881 tcattgtgta cccattcttt caacccatga gcagctccaa atggccaatt ttgataaaaa
     2941 agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat cgtttaacaa tcctgtttta
     3001 ggttcgatat gatagctagc acgccaaggt gatcgtttta cattatcctt aattttatcc
     3061 atctcatctt tagtccaatc agaataagca cgatatcgta tttctcgagg taagttcata
     3121 ttttactcct tcaatttatc tttttacaac tagtatggta aacgttttcc gttgaaaaat
     3181 caatcatttc atttaaaaaa ataaactata tgacaaacgt ttgacatatt tttaagatat
     3241 gctaaaataa atttgcattt cagtgaaccg ttttcacgta aaaataaaaa aggagtgatt
     3301 cgaatgaatt acagtaaagt agcttccgac gtaatacaag cggtcggaaa agataacctt
     3361 attgcagctg cacactgcgc tacacgtctt cgtctcgtac taaaagatga cacgaaagtg
     3421 aatcaaaaag ctttagacga aaatccagat gtgaaaggca catttaaaat tgatggacaa
     3481 tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt atgacgaact tatcaaaaaa
     3541 actggacttt cggaaatttc aacagatgat ttaaaacaaa ttgctagtaa agataaaaaa
     3601 ttcaacccca ttatggcatt aattaaatta ttatcagata tttttgttcc aattatacct
     3661 gcccttgttg caggtggttt attaatggca ttacaaaact ttttaacagc tgctggactt
     3721 tttggaccaa aatcaattga agaaatgtat cctgcgattg aaggtatttc ctcaatgatc
     3781 caactgatgt ccgctgcacc attcatgttc ttaccaattt tagtaggtat ttcagctgct
     3841 aaacgttttg gagcaaacca atttcttggt gctgcaattg gtatgatcat gacaacacca
     3901 gatcttggtg gctcaactga atattggaat atttttggtt atcatgtttc acaaacaaac
     3961 tatgcttatc aagttattcc agttttagca gctgtttgga tattatctat attagaaaaa
     4021 ttctttcata aaaaattgcc atcatctatt gactttacat tcacgccatt attatcagta
     4081 atgattactg gatttttgac tttcacagtt attggccctg taatgttatt gttgtctaat
     4141 ggaattacag acattattgt ttggctatat aacactactg gatttattgg aatgggtatt
     4201 tttggaggaa catattcact tatagtcatg actggtttac atcaatcatt tccagctatt
     4261 gaaactcaat tactttcagc gtggacaaat ggtatcggac atggagactt tatctttgta
     4321 gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat ttgccatttg gttcttaact
     4381 aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg tctcagcact tcttggaatt
     4441 acagagcctg ccttgttcgg agttaactta aaataccgct ttccattctt ttgtgcttta
     4501 attggttcag gaattgctac agcagtagca ggattactta aagtagttgc tgtttcactt
     4561 ggttcagcag gtttcttggg atttctttca attaatgcaa catctattcc attttacgta
     4621 ttgtgtgaat tgattagctt tgtagttgct tttgcaatta cttacttcta cggtaaaaca
     4681 aaagctgctg gtatctttaa agcagaagaa attgctgaac aaacaccagt taattcttca
     4741 aaagtagcaa caacagatcc aatatctact gatgttgaat caacacaaac aaaagtttca
     4801 tcagaaacaa ttgttagtcc catttctggt aaagtgatta gtctttcttc agtaaatgat
     4861 ccagtctttt catcagaagc tatgggaaaa gggattgcca ttaaaccaaa tggaaacacg
     4921 gtccattctc cagtagatgg tactattcaa ctcacgtttg aaactggaca tgcttatggt
     4981 attaaatcaa ataatggtgc tgaagttcta attcatattg gtattgatac tgtatcaatg
     5041 ggaggaaaag gatttacaca aaaagttact actaatcaaa aagttagaaa aggcgatatt
     5101 cttggagcat ttgaccgttc aatgattgag aaagaaggtc ttgatgatac aacaatgatt
     5161 attgtgacca atacttcgga ttattcagaa gtaaatatag tagctgaaaa tacagtcact
     5221 gagggtactg atttacttga aatcaaataa atatatttcc ctgagtaaag atggaatttt
     5281 ctttccaaaa tcaaataata tagattaatc aaatatttga gaataacatt ttatttaatc
     5341 ttttcatata tcaattgtat gaaaaattat tagaatttta aaaaatgata aaaattcaaa
     5401 aagaaaaaat agtagttatt tacttgtcat gttactaaat aaaaaaataa tttaaatatg
     5461 atatatagtc atttatttag tgatttcatt ctaaaataaa gggagaaatg tctatgctat
     5521 acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc cattggcgat gaagaaatga
     5581 cgatcaagga aagagtgagc tttccgacaa cgacacctga ggaaacaatg cctttagtca
     5641 ttgacttctt caaacaatac caagcagatt tagcagggat tgggattggc tcatttgggc
     5701 ctatcgatat ccatcgcgat tccgccactt atgggtacat tacctcgacc ccgaaattgg
     5761 catggcaaaa ttttgatttc atcggtacca tgaaaaaaga atttccgatc ccgatttctt
     5821 ggacaacaga tgtcaatgcg gctgcctatg gtgagtatgt cttcggcagt ggcaaaggat
     5881 tgagcagtgt cgtttactat acgatcggta cgggtgttgg cggcggtgcc ttacaagaag
     5941 ggcgctttat cgaagggttt agtcatccag aaatggggca tatgctcgtc gtccctcatc
     6001 caaaagatga ctttgctggc agctgtccat ttcatggcaa ctgtttagaa gggatggccg
     6061 ctggacccgc catcgaaaac cgcttaggga aaaaaggaca agaggttgca gaagatgatc
     6121 cttattggga aatcgaagcc tcctatattg ctcaatgtgc ctacaataca accttgatgt
     6181 tttctccaga tgtgatcatt tttggcggtg gtgtgatgaa acaacgccat atgctgcaaa
     6241 acgtccacga cgcatttgca aaattggtca atgggtatgt cgaaacacct gagttaagca
     6301 aatatatcgt gaccccagca ttagaagaca acgctggaac gctcggttgt ttggcattgg
     6361 ctcgagaagc ggtcttacat tcataaacga aaaaaggctc ttcgacattt agtgttggta
     6421 gagaaagata caactgaaaa tttagtcata accagttagg cagagtgctt aactggtttt
     6481 tgttttactt ggaagatcaa gccttgaatg agataaattc ttgctcgaaa gttatggaaa
     6541 tttcgatagc cgtaggccac ttgtattgtg caatagtttt ttacaaaaaa aagggggtgc
     6601 ggatattttt ttggaccttt ttaacaaat
//
LOCUS       DAZAFF010000123         6598 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000123 DAZAFF010000000
VERSION     DAZAFF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.127"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG9BB_002639"
                     /pseudo
     CDS             complement(99..1229)
                     /locus_tag="ACG9BB_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG9BB_002640"
     CDS             complement(1878..3080)
                     /locus_tag="ACG9BB_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2838504.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG9BB_002641"
     CDS             complement(3140..3343)
                     /locus_tag="ACG9BB_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFN2838505.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACG9BB_002642"
     CDS             complement(3485..4552)
                     /locus_tag="ACG9BB_002642"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN2838506.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG9BB_002643"
     CDS             complement(4610..4717)
                     /locus_tag="ACG9BB_002643"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2838507.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG9BB_002644"
     CDS             4880..5692
                     /locus_tag="ACG9BB_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2838508.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG9BB_002645"
     CDS             complement(6066..6257)
                     /locus_tag="ACG9BB_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838509.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         2003 a         1331 c         1031 g         2233 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaa
//
LOCUS       DAZAFF010000124         6520 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000124 DAZAFF010000000
VERSION     DAZAFF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.128"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..1026
                     /locus_tag="ACG9BB_002646"
     CDS             19..1026
                     /locus_tag="ACG9BB_002646"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFN2838510.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1038..2042
                     /gene="neuB"
                     /locus_tag="ACG9BB_002647"
     CDS             1038..2042
                     /gene="neuB"
                     /locus_tag="ACG9BB_002647"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFN2838511.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2039..3187
                     /gene="neuC"
                     /locus_tag="ACG9BB_002648"
     CDS             2039..3187
                     /gene="neuC"
                     /locus_tag="ACG9BB_002648"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFN2838512.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3160..3837
                     /locus_tag="ACG9BB_002649"
     CDS             3160..3837
                     /locus_tag="ACG9BB_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFN2838513.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3788..4969
                     /locus_tag="ACG9BB_002650"
     CDS             3788..4969
                     /locus_tag="ACG9BB_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFN2838514.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            4982..5959
                     /locus_tag="ACG9BB_002651"
     CDS             4982..5959
                     /locus_tag="ACG9BB_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN2838515.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            5959..>6520
                     /locus_tag="ACG9BB_002652"
     CDS             5959..>6520
                     /locus_tag="ACG9BB_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFN2838516.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKS"
BASE COUNT         2425 a          820 c         1249 g         2026 t
ORIGIN      
        1 tcataggagg agatataagt gtttaaagga aaaacaatat tagtaactgg cggaactggt
       61 tccattggaa gtgaaattgt tcgtcagctt ttaaaatatg aaccaaaagc tatcagaatt
      121 tatagtagag gtgaagaaaa acaattttat atgcaacaag agctctcaaa tcacactaat
      181 ttgagatttt taattggtga tatcagagac tctcaaagga ttgattatgc atgtcgtgat
      241 gtggatatta tttttcatgc tgccgcaatg aaacacgttc cagcaagtga atataatcct
      301 atggaagctg taaaaacaaa tgtgatggga actcaaaatg taatagatgc agcgatcaaa
      361 aataatgtaa aacattttgt aggtatcagt acagataaag tagtgagtcc tactaacacc
      421 atgggtgcga caaagttatt gagtgaaaaa ttaataatag ctgccaatca gtataaagga
      481 aaaacagaaa caatattttc ttgtgtaagg tttggaaatg tcatggggtc aagtggatct
      541 gttattcctc tttttagtaa acaaataaaa aacaataaag atattacttt gacagatgtt
      601 gatatgacac gatttatgat gtcaataccg gatgctgcaa gattagttct gtctgctgct
      661 atgaattcaa aatttggaga aacatatgtc ttaaaaatgc cggcaatcaa tattttaact
      721 ttagctcaat cattaataaa aatatataat gatacacatg atgaaaaata tagaggtgaa
      781 ataaaagttg taggtatcag gccaggagaa aaattatatg aagagctaat gactacagac
      841 gaaattgaac gctcatatga aaatgataaa atgtatatca ttccaccaca ctatcatgct
      901 actgacgaac tgtacaaaga ttatgaaaaa attaaaatca ctgcatactc ttcagatgca
      961 acagaattaa tgagtggaca ggaatttatt agaatcgcaa aagagaataa tgtcgtgact
     1021 tggtaaggag ttttatgatg aaatcagaag tatatgtaat tgcagaaatt ggtgtaaacc
     1081 ataatggaag tctagattta gctttagaaa gtattgataa agctaaagaa tgtggagcta
     1141 acgcggttaa atttcaaaca tttaaaacga ataagctagt ttccaaaaag gctccaatgg
     1201 caaaatatca gaaggataat cttaaagagg acagttctca atttgaaatg ctaaaaaaat
     1261 tagaattatc tcaatctgat tttattagaa taaaagatta ttgtatacaa aataaaattg
     1321 attttttgtc tactcctttt gatgaagaaa gtgcagactt tctaaaacaa attggtgtac
     1381 aaggatttaa aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata
     1441 tgtatgggtt gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg
     1501 ctgtagattg tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg
     1561 ctagttctga agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac
     1621 ccgttgggta ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg
     1681 gtgctaaggt aattgagaaa cattttacct tggataagga actacctggt ccagatcata
     1741 aagcttcttt agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa
     1801 tatttttagg tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat
     1861 caagaaaaag cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat
     1921 ctcttacaat aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg
     1981 gcaaaaaagt taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat
     2041 gaaaaaaata cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc
     2101 tgagaattta ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc
     2161 ccataaatat ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga
     2221 agttcaaagt ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga
     2281 gataaatggt ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg
     2341 ggatagagga gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc
     2401 acatattcac ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac
     2461 gaaaatagca tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat
     2521 gggggaagat tcttggagag tttttcccgt cggagctcct agaatagata ctattttaaa
     2581 taatagatta cctagtttta gagatataaa agaaaaatat catctaaaat ttgaaaagaa
     2641 aaaatacgtt ttgcaagtat ttcatccagt tgtaaccgaa ctagaaaata tagaaaaaca
     2701 aattttagct ttgatcaatt caatggaaca acaatctcaa ccaattattt gcattttacc
     2761 taatagtgat gctggtagcg aaattatcag aaaaatgtac cagaaatatt taaaaacgga
     2821 tattatttat attacgaatt tagagccgga ggaatacctg actatattaa aagagtgcaa
     2881 atttatgatc ggaaattctt cttctgggat aattgaagct gcatcattca aaataccagt
     2941 aataaattta ggaaccagac aaaatggacg agaacaatct aaaaatatta taaatgcaaa
     3001 tctaaatgaa aatgaaattg aaactgcttt gaataaaata caagatcctc agtttataaa
     3061 aagcttgaaa aatgttaaga acatctacgg tgacggcaac agttctagaa gaataacaaa
     3121 aacattgaat caaatattga atgatgggga agatattaag tggattcaaa aacaaatagc
     3181 ttattaaaat atactagtga tccatacatt attattgggg ctggcggaca cgcaaaagtc
     3241 attattgaaa ccttaagatt agaaaataag ttaatatatg gattttttga tgataagcaa
     3301 aataaaatgc aagatctgaa atatcttggg aaaataaacg aattgaatag ctgggcggat
     3361 agcaaaaaaa aaatctataa atttattgta gctattggtg actgtgtagt aagaaaaaag
     3421 attataaaaa aattgaatat gccaagtgag caatatggaa tagctgttca tccaagtgct
     3481 atagtggcaa aagaaatttc aattggttat ggaactgtta tttttgcgaa tgctgtaatt
     3541 aacccagatg ctgtaattgg agagcatgct ataataaaca caggtagcat tgttgagcat
     3601 gattgtagaa ttaataacta tgttcattta tctccaggtg tatgcttagc gggtggagta
     3661 catgtaggtg taggaactca aataggaatt ggttctcaat gtatccagat gaagaaaatt
     3721 ggatcttggt gtatgatagg cgcagggagc actattgtta aggatattcc ctcacattcc
     3781 ttagcttatg gcaatccagc taaaattaag aaagaaggaa ttaattttga attctagaat
     3841 tttattatca agtcctcata tgggaggaac agaagaaaaa tatgttgcta aggcttttaa
     3901 agataattgg atagcgccat taggtgaaaa tgtggataaa tttgaagaaa gtattacaaa
     3961 atatactggt tctaagggcg gagcttctgc gttaagttca ggtacagctg ccatacatct
     4021 tgccttaaga ctgcttaatg ttggaaaaaa cgatattgtc tttgctcctt catttacttt
     4081 tatagctaca gtaaatccta ttttatatca aggggctgta ccagtgttca ttgactcaga
     4141 acctaataca tggaatatga gccctgttgc actagagagc gcattagaag aagctgaaag
     4201 aaacaaccaa ttacctaagg caatcatcgt agtgaattta tatggtcaaa tggctgatta
     4261 tgaacgaatt ttagctattg caaaccaatt taatgttcca gtgatagaag atgcagcgga
     4321 atcgttaggt tctacttata agggaatttc gtcaggctta tttgggacaa ttgggatcta
     4381 ttcatttaac ggtaataaga tcatcacaac ctcaggtgga ggagctctag tgtctagtga
     4441 taaaaaatat gttgagcatt caagattttt agcaacacaa gcaagagatc aagcaccgta
     4501 ttatcaacat agcgaaatag gttataatta ccgtctaagc aatatttctg ccggtatcgg
     4561 acgtggacaa atggaagtat tgaatgatag agtagctaaa cgaagagaaa ttttcaatcg
     4621 atataaggaa gctttttctg gaattgatag aattgaaatg atgccagaag tggatggcat
     4681 ggtgccaaat cgttggttgt ctactttaac gatcaatgat tataattcta ttgatcaagt
     4741 agttaacgta ataaataaac taaatgaaaa aaacattgaa acaagagctt tgtggaaacc
     4801 actacatttg caaccagtat ttcaaggggt aaaaatgtat taccatgata ataatcttag
     4861 tgtgtcagaa aagttattta atagcggctt atgcttacct tctggaagta atatgacaga
     4921 tagtgaacaa gaatttgtta ttgatgagtt taaaaaaata ttcaagtagg tgtgatattt
     4981 tatgaacata ggaattattg gtttaggttc catggggaaa agaagactta gactcattca
     5041 agaacacttt aatgatattt tactatgtgg tgttgatact aatgaggcga gacgaaagga
     5101 agttgaaaag gaatttggaa taccaactta taatagtcta acagaggcaa aaaatttgtt
     5161 tgaaataaat tcagtttttg tttgtacatc ccctataacc catgaattta ttataaaaga
     5221 ggctttaaat ttagattgta atatctttac ggaaatcaat ttattaaatg aatattatga
     5281 tgaaataatc gaagtagcta aaaagaaaga tagattactt tatctatctt ctacattttt
     5341 gaagagaaaa gaaattcaat atattaagtc taaagtgacg cataataaaa aaatttctta
     5401 tcaataccat gtaggacagt atttacccga ctggcatcct tgggaagatt atcgcaattt
     5461 ttttgtttca aacaaaaaaa ctaatgggtg tagagaaata ttcgctattg aattaccttg
     5521 gattatagat acatttggga aagttaaatc gtttactgta atgacccaga aattatccaa
     5581 tcttgatatt gattattcgg acagtttcac tcttattatt gagcatgaaa ctggtatagt
     5641 tggaacttta agtgtagatg ttgtttcaag agtagctaaa agaaatttaa ctataattgg
     5701 tgaaaagatt ttaatagaat gggaaggtaa cccggattct ctattttcat ggaatgaaga
     5761 aacttctagt atgacagata ttcaattgta cgatgaggtt atgcatgata ataattattc
     5821 acatactatt attgaagatg catatttaga ggagattaaa gaatttttca cgcttttgga
     5881 aaagaaaatg gaaccccaat atacttttga aaaagataag tatattatta acttaattaa
     5941 cgacattgag gtaaattaat gatgaaagta attttttttg ggttagggtc aattggaaag
     6001 agacatctaa aaaatctaat aagctatgga aaaaagaatt ctattaattt tgatataaca
     6061 gcttatagta gcagtggaaa aaaagagttt gaagaaataa cgtatattca aaatagagac
     6121 gatttggata cacattatga tatagctttt attacaaatc cgacttttaa gcatctggaa
     6181 acactaaatg taattgttga aaaagttgat tacatatttt tagaaaaacc tgctttttct
     6241 gagagtattg atcttactca atataattat gattacaaaa atatttatgt tgctgctcca
     6301 ctgaggtata aaaatatcat ggaagttgtt cgtaatagta ttcaaagtaa aaaagtatat
     6361 tctgtaagag ctatttgttc tacttatctt cctgattgga gaaaaggtac tgattataga
     6421 aataattatt ctgcctttaa atctatgggg ggaggagtag aattagactg tatacatgaa
     6481 ttggattata ttataaacct atttggtttc cctaaatcag
//
LOCUS       DAZAFF010000125         6388 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000125 DAZAFF010000000
VERSION     DAZAFF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.129"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..338)
                     /locus_tag="ACG9BB_002653"
     CDS             complement(<1..338)
                     /locus_tag="ACG9BB_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838517.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEF"
     gene            complement(546..1472)
                     /gene="glsA"
                     /locus_tag="ACG9BB_002654"
     CDS             complement(546..1472)
                     /gene="glsA"
                     /locus_tag="ACG9BB_002654"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFN2838518.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1495..2922)
                     /locus_tag="ACG9BB_002655"
     CDS             complement(1495..2922)
                     /locus_tag="ACG9BB_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN2838519.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2953..4167)
                     /locus_tag="ACG9BB_002656"
     CDS             complement(2953..4167)
                     /locus_tag="ACG9BB_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFN2838520.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(4253..4480)
                     /locus_tag="ACG9BB_002657"
     CDS             complement(4253..4480)
                     /locus_tag="ACG9BB_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838521.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(4508..4750)
                     /locus_tag="ACG9BB_002658"
     CDS             complement(4508..4750)
                     /locus_tag="ACG9BB_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFN2838522.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4782..5339)
                     /locus_tag="ACG9BB_002659"
     CDS             complement(4782..5339)
                     /locus_tag="ACG9BB_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFN2838523.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5352..5540)
                     /locus_tag="ACG9BB_002660"
     CDS             complement(5352..5540)
                     /locus_tag="ACG9BB_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFN2838524.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5553..6119)
                     /gene="amaP"
                     /locus_tag="ACG9BB_002661"
     CDS             complement(5553..6119)
                     /gene="amaP"
                     /locus_tag="ACG9BB_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFN2838525.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         1935 a         1335 c         1084 g         2034 t
ORIGIN      
        1 ataaattcaa gatcagccag ttctgtactc gtgaatatac cattaaaata ataacttaaa
       61 aactttaaaa aataagattg accgtacaga aactgtgtga gctcagaaag agcaatacca
      121 tgtttgaata cgagtttata cttatgcttc tctcttactt gttcttttat acaatattct
      181 agacactcgg ctagactttc tagatcgtga aggatcgttg gtgtagtcac ccttagagaa
      241 atagctgttt gttcgatgtc tatttcttgg aattcaaata attgttcaca cagatacact
      301 tttctaaata tatttcgctc gatgtaattt tccagcattc ctgatacacg cctccagttt
      361 ttcttatgat tttatttaat catatctgta aatcgtattt ttgtttcctt tttatttaga
      421 aaagcccttt caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac aagctcacaa
      481 tttcagcctc attgtaggta gctttgagcc gtttggactt ttaccccagc ctctttcaag
      541 aatggttatt caaagatgtc agcgtctagt tctttgacta catctttcaa caattggata
      601 ccagctgcgc tgttaccaaa tggatctagt gccgggctga agacacctat gccgtaacga
      661 tttggcacag ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac accaacatgg
      721 acggaaaact ctcctgattc atcatacaaa ccagcagttg tcataattga tttgacgatt
      781 gtagcacttt cttcagtaat gatttgtttg ccattccaag gagcaattcc tttatttgcc
      841 agcacagcag ctaattttgc tatgcctgta gcggtgacat tgagggagca ttgtctgaaa
      901 taagcatcga gtatctccgg tacgtctcct tcgatcattt gattaccttt catatagtaa
      961 gcaagagagc gattgatatc acctgttctt gattcagaat gatagatttc ttcattcaat
     1021 gtgatattag gatcatcaca gatctctttc ataaatgaca agattcgctc aaatttttca
     1081 tctgcatctt taccggcaat caacgaggtt gttgcaatcg ctcctgcatt aacaaaagga
     1141 ttcattggat gtttgcgatg attgatttcc atattcatga tagaattgaa ggcaaaaccg
     1201 gttggttccg tgttgattgt ttgaaagact ttttctattc cgttgtcttt gattgctaat
     1261 aacaatacgg gaacttttga catgctttcg atggcaaagc gcacatcggc gtctcctccc
     1321 tcaatcagtt gttttgtatc caaatcataa atcgccatac ctaattgttt gggatccact
     1381 tcagctaatg ctggaatgta atccgccact tgtcctgttt gataatagtg gaaatttttt
     1441 ccaatcaatt gttctaattc ttttacttcc atttgaataa ccagctttca ttttttattt
     1501 ttctgcatgc tggaatttat gacggcatgc gtagatcgta aatggaataa ctaagacgat
     1561 aagataagaa acaatcagaa tcgtttcata aatcccatgc tgtccagctg cgatactagc
     1621 aggtggaaca aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta aacaagcaac
     1681 gattactttt acaacttttc cgcctggaac ctgataggct ctttcaagac tgtcatcttt
     1741 aaaaaccagc ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt agattacaac
     1801 agtcaatgac atcgctgcaa gaaatgaaag attattcccc ccgccgctta atgtaaagac
     1861 ggctgcccag attgttgcaa cgatcccatt tgcgatcatc aaatgagcag gaacaccgtg
     1921 tgtgttttct tttgcaaaat attcaggaag taatccttct tctgctgcta cgtgaagtgc
     1981 ttttgaagga gaggttaccc aagaagcaat ttctccgatg acacctaaac aaacaagaat
     2041 cgcaagaatt ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata ggaattctaa
     2101 cgattgaatc acccctgtat taagagacaa accatgttga ggtactgtag tagcgacggt
     2161 caatccgcca aaagtatcta aaatgattgc cagaataacc aatacaaaca ttgctaatgg
     2221 ataatccttt ttagggtttt ccatttcatt tgcatgactg gcagatgttt ctacgcccat
     2281 atatgctaaa ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa aatcaggaat
     2341 caatgttttc atagacagta tcgtttgtag tggagcgcct gaagcgacat aatggatccc
     2401 taagccaaac aagactaatg ctggcaacaa gataccagct acaaaaccga tccgagcaat
     2461 caatgaagtg tatttggttc cttttaactg tgagagtaaa atagcccaga aaataatcaa
     2521 taggattgcc aatttcagat atttattgtt ttgtatagca ccaatacccg ttgcatagga
     2581 taatgcacca gttaagaaat acagcatggt caagtagcca actgttatct caaaccattg
     2641 gaaaaagatt gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc aagtatagac
     2701 accgcctttt tcccatcctt tcaccgttgc catctctgct gcgcacaatg ccacagggat
     2761 aaaccatagc aaaccgccta ataacaagaa aaatactaag ctgaagcctg aggtcgcgaa
     2821 tgtgggatat tcatataatg aaacgaccat tgagatggtc atagccaaaa agccaaagaa
     2881 cgttaatttt tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc ctcttttcta
     2941 tttgaatttg tttcaattga gtttgttgcc aaagaagaaa aagtccaatc cttcttcttg
     3001 ttcttctttt gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa tcactgttgt
     3061 cagcacatag gcatacacgg cagtaatgat gacaccaatc acttgaatcc ctaagaagta
     3121 ccagctgctt ggattcgtta gtaaatggct gttagatgca aagatcccta tcagaatcgt
     3181 acctgtgaat ccgcccatgc catgaacacc ccacacatca agtgcatcat cccattgttt
     3241 ctttttacga aaatcaacag cagcatgaca gacgataccc gcaatgatcc caatcagcat
     3301 ggcacttttt gcttctacat aaccggcaca aggcgtaatg gctgccaatc cagcaacgga
     3361 tcctgttagt acgtcgacaa aatcaaagcc atttcctttt acttttgcaa tgatcagcca
     3421 agtaatcatc gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga ctgcttgttc
     3481 atctgctcga agtgcaccac cggaattaaa accgaaccag ccaaaccaaa gcagtcctgt
     3541 accgatagca gctgccataa gactgctatg ctgacttctt tctcctaaag gaatctttct
     3601 ttgaccaagt gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt ggatgactgc
     3661 tccaccagcg aaatctctaa atcccatctg atccaaaaat ccgccacccc atatccagtg
     3721 gcaaacaggg aaataaatca gcagattcca aaaaataaca agtataatat agccttttaa
     3781 attcaatctc cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga taaacatcag
     3841 ttggtaaaga aagaacatta aaaatggaat cgttgcacca tgaaacatgt tggggaagct
     3901 tgtaacatgt ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc cgatatcccg
     3961 tccaaaggcc aatccgaatc caccaaatac ccacataatc gttgttatcc caacggagag
     4021 aaaagattga gacatgatcg ttagtatatt attcttccgt gctaatccgc cgtaaaaaaa
     4081 agctagacca ggagtcatca ggcaaaccat tgctgtacat aaaatcatga atgctgtatc
     4141 tcctgtattg ataggtaatg tattcatttt tttcgcctcc tttctacgat tacgagtata
     4201 aaagaaggtg tagttgtttt tttcttctgt ttttttgaat taatacttgc atttataaaa
     4261 gacagacaat taataaaagc ttgatgtgtt gtgctctttt tcgtttttca cacaaataaa
     4321 gacaaaaaat aaacggtctt ctttttatag aaaaccgttt attttcgtct ttttttattt
     4381 ttaaagatat tttttcataa ggaataaaga atacttcata taaaatatat aaaaaagaac
     4441 cgcagcgagt gtcgctacgg ttctcaaatt tgttcttcat ttcaacataa gtttttcttc
     4501 aatttgctta taatttacgt gcaaagaatg aaacgactaa tactaaaatg attgcaccga
     4561 tcaatgatgg aatgatagcc attcctgcaa ttgttggtcc ccaagttcct agtaaagctt
     4621 gtccaattgc tgaacctact aaaccagcaa tgatgtttgc gatgatcccc attgatgatc
     4681 ctttattcgt gattgctcct gcaattgctc caatgatacc gccgacgatt aatgcccaaa
     4741 taaatcccat tttaaattcc tcctaactga cttcttactt cttattgtac acgtggttcg
     4801 ttattttctt tgactttttg tgcacctttg cctacagcag attttgcttt atctgtttgg
     4861 cgtgaagcaa attgtcctgt ggattttgct gcttctgtta ctttgtcttg tactgtttct
     4921 tgatcttttt ggtattcttc ttgtgttttg atgtctgcaa cattcacatt cacttcgata
     4981 acatctaaat gtgtcatatt gtttacttca gtactgatca attctttgat ttggttgtaa
     5041 atatcttcga tgtctttacc gtattctacg atcacatcta gatccacagc tacttgtttc
     5101 ttacctactt ctgtattgat acctgctgtt acgttatctg tgttgactaa tttttcagcc
     5161 atatttgaga aaaatccgcc atctaatgtc aatagaccat ctacatcttt caatgcataa
     5221 ccgatgattt tttggatgac tttgtcttca taagtcaatt caccattcat catgcggttt
     5281 tctccttttt catttgccaa aactgatcca tttgttgtat tcatgttttt gttttccatc
     5341 tttgtttcct cctatgattt aaaaaattga tcaactattc ctgactgttt caaataaaaa
     5401 ccgattcctg cgccaattgc tgtcatgatt atgatcaaca atgtcttgaa aaatccaatc
     5461 gttaaccata aaacagctag cagtagtcca agacccccaa agataatagg gagcttgtat
     5521 gctgaaaata gttctttcat tatttggccc tcctattcca ctcgagagtg cttgtagtct
     5581 cttgtatctt cttgttcttc ataaccgctg tatgtgactg ccactttgac cgtttctttt
     5641 gttccgagta ctttagttac ttcttcttcg atatcttgca tcaatacacc tgttttccct
     5701 actaatgaag aagttcgttt caagtctccg ttgacatgga tatggattcg atttttcgtt
     5761 gaacgtatcc gtactttagg tgaatgaatg aactcttctt cgtgtaaatg ggaacgtacc
     5821 aacccttcga tcgctttttt gtcgattgtt agtttgccat cttcccgttt cataacgaaa
     5881 gttcctcttg actttggata gaaaagaacg actagcaaga tgataatagc aagaacggca
     5941 aatactacag ctgcccagaa aatatattgt tgcatgtagt aattcgttaa agtaaagaag
     6001 cgaaatcgtt ccaattgttc aggcataata tacggcgcat tgatcaacgc aatcgctaga
     6061 aatactgaaa gtagcagtaa actcatgata acaccgatta tttttaaccc cttattcatt
     6121 ttccacctcc tataaactta tttttaccct gtaaataact ctaccgacaa tcacagtata
     6181 gatagcgaac agttagcata gcatcagtaa ataaagcgct accaaacatt gctggatcat
     6241 aacggcgttt tattgccagt ctgatactga aataacaatt gcttacttat tctctattta
     6301 cttttgattg ctggctatac ttaaaggata ttattcaaga ttaaaaattg caaagattat
     6361 gctcagttat tattacaaga ttctattt
//
LOCUS       DAZAFF010000126         6387 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000126 DAZAFF010000000
VERSION     DAZAFF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.130"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1175
                     /locus_tag="ACG9BB_002662"
     CDS             330..1175
                     /locus_tag="ACG9BB_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN2838526.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ACG9BB_002663"
     CDS             1456..2676
                     /locus_tag="ACG9BB_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFN2838527.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ACG9BB_002664"
     CDS             2673..5393
                     /locus_tag="ACG9BB_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2838528.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="ACG9BB_002665"
     CDS             5377..6321
                     /locus_tag="ACG9BB_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFN2838529.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2379 a          987 c         1231 g         1790 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttc
//
LOCUS       DAZAFF010000127         6337 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000127 DAZAFF010000000
VERSION     DAZAFF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.131"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(126..1892)
                     /locus_tag="ACG9BB_002666"
     CDS             complement(126..1892)
                     /locus_tag="ACG9BB_002666"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2838530.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1852..3606)
                     /locus_tag="ACG9BB_002667"
     CDS             complement(1852..3606)
                     /locus_tag="ACG9BB_002667"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2838531.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3716..4285)
                     /locus_tag="ACG9BB_002668"
     CDS             complement(3716..4285)
                     /locus_tag="ACG9BB_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFN2838532.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4303..5718)
                     /locus_tag="ACG9BB_002669"
     CDS             complement(4303..5718)
                     /locus_tag="ACG9BB_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2838533.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5729..>6337)
                     /locus_tag="ACG9BB_002670"
     CDS             complement(5729..>6337)
                     /locus_tag="ACG9BB_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFN2838534.1"
                     /translation="FLGKGNIFQVGCFIIATVLVLMFRKIKTCIYFILIYSVLFLVMK
                     IIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVYTSNELLSSLEKIHLPKKIML
                     SITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQYLEFLLVPVLIRATMIAEEMT
                     ANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFLSILI"
BASE COUNT         2150 a         1165 c          846 g         2176 t
ORIGIN      
        1 actaattcaa tcatactgat tctttcgtgt attcaaaatt agatgctgtt taataaaatt
       61 attctttttg aaaaagaatt ataactatat cccaaaaatt gtaggttaat ttaaagccta
      121 tttcatcatc gtgattgagc ttctttttca tagacgaatt tctcagatag tttagttttc
      181 tttatgagat ttttgtatat ttctgacctt cgatacaact cattatgagt accttgatca
      241 actagttttc cttgatccat cactacaatt ttatttacct tttcaataga cttcaagcga
      301 tgcgatataa ttagtactgt cttatcttgt attaaacgat ttaaactttc ctgaatacgt
      361 ttttcatttt ccgcatctaa actggcagta atttcatcta aaattaaaat tggcgcattt
      421 ttcaaaaaag cccgtgctat ggataggcgt tgtcgctctc ctccagaaag tgaacttccg
      481 ttttctccga ccaatgtatg ataaccgtca ggtagttttt caataaagtc ttcgcagtta
      541 gctaaacgag ctgctttttt cacttcctca tctgacgctt gactatttcc aatacgtata
      601 ttttccaaaa tacttgtatt aaacaaagtt acctcttgaa aaacaatagc gattttgcta
      661 aacaaagaag ctggagatac acgtttaata tcatacccat caattaaaat acagccttca
      721 tcataatcat acagtctaga cactaatttc aagatactag ttttcccgga accactagca
      781 cctactaacg cagtcacttc tccttgttta gccgtaaaag agatatgatc taaaattggg
      841 gtattgttat catatgagaa agagacatct cgcaactcaa tgtcaaaaga tttcagagga
      901 ctatctgatc cctcttggat actggtttcc ttcatagctc taattcgctg tattttagga
      961 gctaaataaa atatttcaag cactgcttct ttcatagaat cgaaggaatc ctttattttt
     1021 attgcggcta gtaagtaacc aacgacgtag agtatagtca cttcacccgt taacagtaag
     1081 tgaacaccta ctagaatgac tactgctaaa ctaacatatg agaaaataga ggataaggcc
     1141 atgactgaaa aagtacttaa ttctaccttt aaatgaatcc tttcactctc ttccattttt
     1201 ttgaaaagtc tgtcttgaac ttttttagat agattaaagc tattaatctc ttgctgcaat
     1261 tctatagttt cttgaaattc ttccgagttc tctctcaaag tatcgtaata tttagtattg
     1321 gctttcgttt gggatttctt tgataacaag attaagacaa aactaaataa cgttggcaaa
     1381 ataacagcta atcccatttt gacattacca accaaaagca tcactgaaat aaaagggaaa
     1441 aacagagcca taccgccggc tttaggtatt gcatgactca tcgcatgctc aataccttca
     1501 acgtcagaca tgatagtttg agataaatct gataaattat gttttgaaaa ataggataat
     1561 ggtaaatttg acaaatcgtc tgcaatttgc actcttaaat gcgcactttc tttataagta
     1621 ctattatata agttttcata ctccctatct aacaaccaat acatgaaaag caaagtaaca
     1681 aacgatacgg caaaataaag ccaacggggc tttgtatttt ctaaaactaa ttcgtctatc
     1741 agcatcatta taatgaatgc tggtaccata ttaatgcagt atacaaaaaa actagaaata
     1801 gaagctttag ttaaagcttc tgcgccctga tcacttaaag caaatttgtt tttataaaaa
     1861 cttttcattg tcaagcctcc aatcatttgc attctcataa ttagtgacca attgtttata
     1921 cacttgatgt ttagccatta attcattatg atttccacgt tctactattt tcccttctga
     1981 caagacaaga atttcatcta cagatttaat gctggataat cgatgagcta tcataataac
     2041 tgttttttct ttcatcagat gttgaaaagc cttttgtagt tcatactcat tatctggatc
     2101 aatagatgca ctggcttcat ccataataac aatctttgca tcttttaaaa ttgctctggc
     2161 aatagctata cgttgttttt ctcctcctga cagataaact cctttactac caattaaggt
     2221 atgctctctt tcgggaaatt tttctaaaat cgattcgcaa ccggctaatc ttagcgcgtc
     2281 aaatacttta cttgctggcg cttgaggatt tgctatagaa acgttatcgt aaatactctg
     2341 attgaatagt ttcgtatctt gaaagacaaa agcaattgtg ttaataattg tgtcttttga
     2401 atagtgattt aaaggcttac caccaatttt aatctgtccc ttatccacat tgtagaaacc
     2461 tgaaattagt ttagctatcg tactctttcc gcttcctgat tttcctacta atgcataaat
     2521 tcttttttgt tctaaaacta agttgaaatt ttctaaaaca cactcttctt tgtatgaaaa
     2581 gctgacattt tcaaatgcaa tgtcacaatt tttaatctgt gtctcgttcc catattttag
     2641 ttggtcttgc ttcatttcta aataaaggga gtctagtgtt tcgactgcat atcttgcttc
     2701 aaacaagtgc atacttacat acattattct catgaaagaa acaaaaataa ttccacttaa
     2761 aaaaaacaac atcaacaagt caacagctaa tatcttggga gtggtaagag aagttataaa
     2821 aaaggttata ggaactatca aaatgcaaac tatgccaaaa aatagccatt gaaaaagaac
     2881 aaaaggtgtt ttcccactca aagaatactt atatgcaaac ttagcataat cgttaattgc
     2941 tttattcaag gctttaaatg atgttacttt agcaccaaat atctttataa cttgaattcc
     3001 gcggatatat tctaccgttt cagagcttaa cacatctaac gattcttgat atttttgaat
     3061 aaaatttgta ttctctgtca ttttctttaa caagaataca ccaattacta acatggcgat
     3121 tagaaccaat ccaattttaa agttaatgaa aaagcctaag gcaactgata aaacaggtgt
     3181 tactatggct tgagcactat ctggaatcaa gtgagcaatt gccatatgtg ttttagccgc
     3241 gttgtcatca attgtctttc gaatatatcc cgaagaattt aaatcaaaaa aacgaaaact
     3301 tgcttctgtt aaaccgctaa ttcccttttt tctcaaatta gtctctaagc gaaacccaag
     3361 gatatgagaa aataagccag aaaaaatata aaataaagct cctaaagtta accaagcaac
     3421 agtttgaacc gcataactct tagctaaata ttcatctcct acaataatta gagactgaag
     3481 aaatttgtaa atactgtaat atccatagac tgttaatacg acagaaaaat cagaaaataa
     3541 agtggctaac catccgtagt atttcatttt agggacataa ctaaataatt ttttgtatac
     3601 tttcattttt tcctcccccg cattcaattt cttctcttat tcgtttacaa ctgttaatgc
     3661 tttccgtaat agcgtaaatt agagatgtac aaacagataa aaggtaaact acgttctatt
     3721 ttttgtattt ttttaagact tttcttccaa tcaatacacc aatcatagat aaacttgcta
     3781 caactaacac tgaaatgatt ccccatatac ctaccgcata gtggatcatt gcatccattg
     3841 ttgcagcaga ttgtccttta tctatccaat cttgacgaaa acgatctgta aagaaccata
     3901 taggaatcca ctgtccggca gcactgccta attgcattag agcccaagac gcaacttgag
     3961 gaattgctcg atcatatcca aatacagata gaataatatc agctaagatc gccatactaa
     4021 tcgaactgat aaaccaaggt aagtacgccc cgcccattaa cgtaaaaaat aacaataaaa
     4081 caattgtgta aatagtaaaa attccttttc tagggtagtt atagctaata taaacaaata
     4141 tgattccaga taataatcca aaaatcccgg gggatatgct gtgaccaaac gccccaccaa
     4201 tactactaac cattattgaa aaaaagtaaa ttactaggga aataactgta gctaaaccaa
     4261 taaaaatata atcttttgtt ttcatatata ttctcctcaa ttctatttcg ttatgtcatt
     4321 aaaaatctgt tctatcaaag ttgaatcttc tactctaagt tttatatctt tttttattcc
     4381 accatttttt aaataaataa ttctattgca tgttttataa agaaattccg gatcatgagt
     4441 agctataact actgcaccct tttcttttaa gtctttaata aggtcagcaa tcctttccat
     4501 attaatataa tccaacccac ttgtaggttc atcaaataga aataaattag tttttgacaa
     4561 tgaagctata gaaaccaata atctttgttt ttctccaccg gataaatcaa acggtaattg
     4621 atctttcatt ttataaagtt ccattttttt taaaattttt tcttgctgat ctttactaac
     4681 tatttcattc cccagggcta actcgctcct cactgattct gtgaaaaatt ggtaatccat
     4741 ttcttgcatt actaaaaacg gcgatgacaa atttttttta ttttcgatta tctttccttt
     4801 ggtagggcga ataatcttca tgagtattct tagtaaggtt gttttcccca caccgttttt
     4861 ccctaccaaa ccaataacat ctccaacttt gacatcaaaa tttatgtctt ttagaatatt
     4921 ttcataagta atttcttcaa cttttaatac atctgcactt accttttcga tatagggaac
     4981 ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg ttattgtttt cagtttttaa
     5041 caactgaata gttgtagttt ttacagttct atttttaaca aatagaactt gatcaattaa
     5101 ttctttcaaa tagtaaaaac gatgttcagc tataacaata gtaaatcctt tattttttaa
     5161 atcacaaagt aagtcagcaa gtttcttagt cgctttgaag tccaaatttg aagacggttc
     5221 atccaatata attaatttag agtttagcga taaactacat ccaatagaaa ttttctgccg
     5281 ttcaccactt gatagcgaaa aaatgttttt atctaatata tttgaaagat ctaataattc
     5341 agctaattca ttgattcttt tatccatatg ctcttttgta aatccaaagt tttccatcga
     5401 aaatactaac tcactcgttg tatttgttgt aaagaattgt cctctaggat cttgacttac
     5461 gtagccaata ttttttgata tactttcttg tgttaaggat tgtagcgagt tattttcaaa
     5521 taaaatttcc ccaaccaatt ttccgtcata aagtcttgga ataagtccat tcattaactt
     5581 taatagtgtg gatttcccac tcccagaaga accagtaatc aatgtaagac tgcctttttt
     5641 tattgaaaaa ttaaggtcaa caagcgcttg ttccttaaca tccttatatg aaaaagaaac
     5701 attgttaaat tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa aaaatagtaa
     5761 cggaaaacag gatgatatag tctaataagc tcatgtttgt tttatagtaa gaacttcttt
     5821 tcacaggaga ttctatcccc tttgttattg cattagcggt catttcttca gcaatcatag
     5881 ttgcacgtat aagaacggga actaacagaa attccaaata ttgcagaggc tgaaaaaaag
     5941 acaagcgaat gtttctcatt tttaaagatt ctttaatcat tcttatttca gaacgataag
     6001 tcggaaagaa tctgatcact actgtaatac ttaacattat ttttttcggt aaatgtattt
     6061 tttctaaaga ggataataat tcattgctag tatagacact aaccaacaac catgagatca
     6121 ttacgaccgg caacattcga aatacaatat ataaggatgt cgcaaaaaat atagtaaaaa
     6181 actctatctt gatagcatca ataattttca ttactaaaaa cagaactgaa taaatcaaga
     6241 tgaaataaat acatgtcttt attttacgaa acatcaatac taaaactgta gcaataatga
     6301 aacaccccac ttgaaatata tttccctttc ctaaaaa
//
LOCUS       DAZAFF010000128         6078 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000128 DAZAFF010000000
VERSION     DAZAFF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.132"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..541)
                     /locus_tag="ACG9BB_002671"
     CDS             complement(254..541)
                     /locus_tag="ACG9BB_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN2838535.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="ACG9BB_002672"
     CDS             complement(561..785)
                     /locus_tag="ACG9BB_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFN2838536.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1455)
                     /locus_tag="ACG9BB_002673"
     CDS             complement(1240..1455)
                     /locus_tag="ACG9BB_002673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838537.1"
                     /translation="MEKMVAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKG
                     NKKGELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="ACG9BB_002674"
     CDS             complement(1425..1958)
                     /locus_tag="ACG9BB_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN2838538.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="ACG9BB_002675"
     CDS             complement(1979..2923)
                     /locus_tag="ACG9BB_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFN2838539.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3572..3658
                     /locus_tag="ACG9BB_002676"
     CDS             3572..3658
                     /locus_tag="ACG9BB_002676"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN2838540.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3779..4468)
                     /locus_tag="ACG9BB_002677"
     CDS             complement(3779..4468)
                     /locus_tag="ACG9BB_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838541.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4475..4753)
                     /locus_tag="ACG9BB_002678"
     CDS             complement(4475..4753)
                     /locus_tag="ACG9BB_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838542.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4713..5627)
                     /locus_tag="ACG9BB_002679"
     CDS             complement(4713..5627)
                     /locus_tag="ACG9BB_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN2838543.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5609..5989)
                     /locus_tag="ACG9BB_002680"
     CDS             complement(5609..5989)
                     /locus_tag="ACG9BB_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFN2838544.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1608 a         1121 c         1026 g         2323 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gagagcggtt ctgaatgctt atctaagccg ttttaagaca gtcttggata aaaatcctta
     3421 tctagcttta aagcgcctta caggcggtca gggcaagccc taacaaccct aaaataagcg
     3481 atacttaaaa acaccacaca agtttaattt tttaagatat aataacttgt cgcctatccg
     3541 acagtcttgc gactggaagg aggtgtatct aatgtttgaa ttgttcattt gcccccttct
     3601 tatagaagtt tgtgtctctt tattcagtta ttggttgaat aaccgaaaca agaagtaaaa
     3661 tcaataaagg cgacatcaac ctgaaaaaaa gccccagaga atccgacctc tctggggctt
     3721 tcgtgtatct aatgtttgat catttgcatg tttagtttaa catgattgcc gatcaaattc
     3781 aattttgctg ttgctgatct aaccattcct tgacactttt tagataattt aatgccgttt
     3841 cttttttcgt tggattttgt atgttcgttg cttgctgaag cagtgcttta taacgttcgt
     3901 aggtaactga ttgttgccgt tcttttcgtt cctgttcact agttgaaaaa gggattgtat
     3961 cactgaataa attataacaa gcttcttctg tctgcgtatc tttaaaatat tgcacgtctt
     4021 ttttggttcg ctcatagttg tcttgaacct ctttttgctg tttttctggc aagttttgat
     4081 aaagacgagt gagtttttcc atgtttttat ccgtcaaacg tttttcttga ttcacaaaaa
     4141 tatcgttcat ttcctcataa caagtggtaa actcttccat tttttgtgtt aacacttgct
     4201 cctttttctt tttatcacat gctgcttcat acttttgagt gatatcccct aataaaatac
     4261 ttcttgaaat acgtttattt ccctctaaaa cgccgaataa accacttaaa acgattgata
     4321 atgtaaatat aacccctggc aataaaaacc tcttaaatcg aattttagag ccttttaatg
     4381 aatatcttgt ttgataagcc ttatagctaa gccaaataat tcctaaaata ctggcaaaaa
     4441 tgagtagata gggtaaatag aatgccattt ctatttattc tccccatacc agccaaaagg
     4501 ctaacagtgt catgattaag caacataagg gggcaagaat atccattgat cgctgcataa
     4561 ttactttttt ctgttcttta tttcgctggt ttaatagcgc atggtggaat tttttgactt
     4621 gtgaatcaat tgtagtttgt attctttctt tggtataagg tgcaagaaaa gcacgttgtt
     4681 tctttatttc attttctatg tcttgtaacg ttttagaacg ataattcttg ttggtagtct
     4741 gctcctcttt caacgtgttg gtcatggttt cttcgagtcg gtctaatttc cggttgttca
     4801 gtcgtaagag ttcgttgttc tcttcgtact tcttttgcag tttcgcgaat gctagctcga
     4861 gctcttcgta gctcgttggt tcgttgttga ttgtaggctc gttctcgtct gtccagctca
     4921 tgtaaaatcg tctccttttc gtaatccttt cctagtttgt tggatcgtac ttttttcttt
     4981 ttgtggatca attcgtaagt aaaatttttc ccacgattca agcaatgaat gccgaatgtt
     5041 ttaagagaat cacaaaattg gttatacgtt cgagtttttt tgtttttcat tgtttgatca
     5101 atcgcaaacc gaatttcgtc tttccaaaca ggttcatttt tttcttttaa tttgatttca
     5161 gacatggttc gtttttcggg tttcttccct cgttcagtga ttgttaagtc atgttcaaga
     5221 caaatctcgt catttaggtc ataacatttg tttaaaaatg cttgaccatg ggcttgaaat
     5281 ttgttcccag tttccagatt gacggagtta atgacaatgt ggttatgaat atggtctttg
     5341 tctgtatgtg tgtaaatcgc gatttggtgg tcaggggcta tttttttcgc atactctgcc
     5401 cctagttgat tggctttttc agcggtaatt tctcctggtc gaaaagactg gatcaataag
     5461 tgtgcttgta cacgatcgtc ttttccatac agcatgcgaa tttgtttcat ttcgcttttg
     5521 gcataatcaa tgtcacagtt tatccctgtt ttgacggttg ctcgtggttc ggcgtaattg
     5581 atcgctcggc tacaagaagt ggttcggctt actttaactg ttgccatatc ttttgtagct
     5641 ccttttgcat gatttttagg ttttcggata agcgttgggc gtcgccagga tcaacgtcgt
     5701 gttggtgtgt gttgcaccac ttggcaattt gattgatatt cccgccagct ttggctaaat
     5761 cgcgcgcgat tgcctgagca atgtctggcg ccactttcgg tgtcaccaga cgcgcgtttt
     5821 gtgccttggc tttcacgaaa gccggcacac tcatttgcaa agcttcagct gactgcttca
     5881 gctttgcaaa ttcggattcg ctcactcgaa aactgatttg ttttggctcg gatcgatagg
     5941 tatatttttt agaatgattg ctagccaaat attgattttg ttcgctcata agtcccaccc
     6001 tttcagtacg tcctacggac gtatcgttcg caataccatt gtacctaaaa aggtacaata
     6061 agtaaagctc actaaagc
//
LOCUS       DAZAFF010000129         6075 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000129 DAZAFF010000000
VERSION     DAZAFF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.133"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..2075
                     /locus_tag="ACG9BB_002681"
     CDS             168..2075
                     /locus_tag="ACG9BB_002681"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFN2838545.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(2616..5894)
                     /locus_tag="ACG9BB_002682"
     CDS             complement(2616..5894)
                     /locus_tag="ACG9BB_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFN2838546.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         1764 a         1235 c         1145 g         1931 t
ORIGIN      
        1 actacttgat tcagttggga tcgcctcctt tttttcatta taagtgaagg attgaaaaaa
       61 acaaatcagt ttgattgttc gaattttcaa ttttttatgt tctatttgtt aattccttca
      121 ttttgtaaga taatgggcac acgaaacaaa aaaggagcga tcgatgaatg aacaaagaac
      181 gattgaaaca agatttctat gaagcagtca atgaagaatg gcaaaaaaca gcaaaaatcc
      241 ctgcagataa accagctaca ggcggtttcc aagatttagt tgacgggatc gaccaacttt
      301 tgatgggaga aacagacgat ctgcttgccc atccagaaaa agtgtcttca gaaaaaatga
      361 aacatttcct tgcatattat caactggcaa acgattatga ccgaagaaat gagttgcatg
      421 ctcaaccgct tcaacctata atcgaaaaag ttgaatcatt gcagtctttc gacgaattga
      481 ataagcaatt tccagaatgg ctgttggata cattaccatt gccatttaat ctggatgtag
      541 atgcagatat gaagaatgcg caaacgaacg ccttctttgc ttatccgcct tctttattcc
      601 ttccagataa gacgtattat gcatcagacc atcccaacgg tgcacaacta ataggcgttt
      661 tctttgatat ggctgttcag ttattagagc ttgccggcaa agaaaaaagt caggcagaag
      721 caatcacgga acaagcagtg caatttgata aattgattgc tcctcatgta aaaagtgcag
      781 aagaaaacgc agattacagc aaaatgtata atccgcaatc ttttgataca tttattaaat
      841 acacagataa actggacttg aaaaaacttg tcacgggttt gattgggaca actccagata
      901 aagtgatcgt aactgatcca gtgtacttcg atcatttgaa tgacatcctg acagaagctc
      961 attttccgtt attcaaaagc tggatgatca ttaaaacagt acgttctctt agtggttatc
     1021 tatcagaaga attccgccaa gtcagcggaa tctattctcg cgtattgtct ggaacagacg
     1081 aagcaatgcc tcagaaaaaa gcagcgtatt accttgcctc tggacaattc gatcaagtag
     1141 ttggcgatta ctacggaaaa aaatattttg gcgaagctgc taaaaaagac gtagaacaaa
     1201 tggtgaaaaa aatgatcgcc gtttataaaa aacgtctaga agaaaatgac tggcttgggg
     1261 atgcaacaaa agaaaaagcc gtcataaaat taaataactt aggtatccaa gtaggctacc
     1321 ccgataagat cccagcatta ttcgaccagt tcaaaacagt acctgcagat cagggtggta
     1381 ctttgctttc aaatactctt gttttcagca aactcacatt aaaagatcgt ttcagcaaat
     1441 ggaacaaacc agtggatcgt tcagaatggg aaatgagtgc tgacacggtc aatgcttatt
     1501 atcatccatt tcggaatgtg atcgtctttc cggcagcgat ccttcaagca ccattttaca
     1561 gtctagaaca atcaagctct gctaattatg gtggaatcgg agctgttatc gctcatgaga
     1621 tttcccatgc atttgataat aacggcgctt tatttgacga atatggcaac ttgaacaact
     1681 ggtggacaga agaagatctt acacaatttc aaaacaaagc acaagcaatg atcgaagaat
     1741 tcgatggtct gccttttgct gacggaaaag taaacggaaa attaaccgtt tcggaaaata
     1801 ttgctgatgc aggcggtcta agctgtgcat tggaagcagc aaaaacggaa tcagatcttt
     1861 ctatcgagga attctttgtc aattgggcaa cgatttggcg tacaaaagca aaaaaagaat
     1921 accaacaact tttactgcaa atcgatgtcc atgcccctgc taaactccga gcaaacgtgc
     1981 aaccaaaaaa ccttgacgaa ttctatgaaa catttggtat cacggaacaa gacgctatgt
     2041 atctagcgcc agaaaaaaga gtgcgaatct ggtaagaggt tgtgccagaa gtggtcagct
     2101 tcaagaaata agaaggaatt tgggaaaatc gcttctcaaa tttttgtgac atttcggctt
     2161 atttttcgaa gaagttactt ctggaatacc atgtattaga ggttgtgaaa aagctgctta
     2221 gtttcaagta gtaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttga
     2281 cttactaccg agaaactggc ttttgaacac cgtttatcga gaggttgtga aatcagcgtc
     2341 ctgacctgag tattagagaa aaaaacggaa aaatagttcc tcatattttg ccgtttgttg
     2401 agctaatacc gagggtcagc tgattgaaca ccgtttagtg gagagattgt gaaaaagctg
     2461 tcctgcatca agtaataaga acagccaatc aaaagcagca aggagaacct catctataaa
     2521 aagatagaag gaagtgagac atctattggt ctcatttcct tctattttta ctttcctcag
     2581 tcaattgttt tacatcataa aaggagaaat tccacttatt tttttcgctg tttcatgaca
     2641 taaacaacta caccgattaa caaaattcct ccagctaatg ctagcaacgg gttattaaat
     2701 tctcccgttt ttgggagtcc ttgagtgcca tttttttcac tttcttttgc tgtcctatcc
     2761 ggatgagaag gttttccatt tggttttcca tttggtgatt catttcctgg attttctctt
     2821 tctggtttgt catttcccgg gggcacatcg gcagtttctc gcacagtgat cgctttttgc
     2881 tgggtaacag attctgtatg agcagaccat ttttccccat caaatatttc cgaacgataa
     2941 gtgacttcta gcgtatattc tccttttgaa ggtgctgaaa aaatagcggt atgattagaa
     3001 aattgagcac ttgactgtgt ttcaacgatt tggatctttt ctgggaaata acgcatatct
     3061 tcaaagacag gcgttccttg atttggtgtg aaagttgaca caaaaactgt aaaaggcttc
     3121 agttcaatga tatttccttc tgcattctcc gccacaccat ttgcttcttg tataataatc
     3181 ggattatttt gttcatttgg ttttgtctga tttggttctt gtacgattgc atactctgga
     3241 tattccgaag acggtgaggt ttctccgtca aaaatagcac ctacagataa tggaagacca
     3301 tccactcttt ccttttctgt ttgacgattc cccggtttaa tctgcatcag tccatgaaca
     3361 tggatacttc cattttcata gcgcgtaaca ggctgctgac cttctttcga acatgtattt
     3421 agagactcga tccaatcttt tgatatggta tgtccaagag tattttctga ttgtttctct
     3481 cgttcattcc aatagtagtt caacgagtgg tagatttgat cttcgttttg attcaatggg
     3541 cggatctgct ctctcatctc aagattctct gttcggaaag ataagatacc agaagcatgg
     3601 tagtctgtgt ttcctgctga agaggtataa agagccacat tatatgaacc gttattaaaa
     3661 cttgtactgc tattgacgat aatatctgtt ccgctattgg aatcgattcc tttcgcaaga
     3721 ttttcgaaag acaaactatt gatcaattca tgagggcccg ttaaagaaga accgcccatt
     3781 ttgaatccat taccgttacc ttctccctca attcctggta cccagccgtt tcgataagca
     3841 actgagtttt tgatgattac ttttccgatc gaacctgttt catttttagc aaaaaggtcc
     3901 caaccgtcat ctgcattatg atacgcgata caaccgtcaa atacatttcc ttctcctatt
     3961 gtcagtttgg ccgcaaatcc atcggcatct tcaaatcctg gatcggcatt ttcaaaagat
     4021 gtacagttct tgactaagtt atgcgcgggc catgcttcga aaggatcatt tgagctaccg
     4081 ctgatttgga ttcctgtatt tccattccga taagcatgaa tctcttcgat caaattatga
     4141 tttccagaaa tctgtaatcc tttttgcata gctgcactat ttgtaatgtc aaaccctcgt
     4201 aaaccccagt agtgactgaa cagtgtcatc ccatttccag ttcctttaaa atcaagtaca
     4261 ggacgcttgt tttctggatt tttctctgca atcaactgaa tcgggttttc tcttgtcccg
     4321 ttaacgcctt ttggaatcgt caatcctctt tcgaaggaat aagttccagg cgccatgacg
     4381 attgtctgcc cagcatatgc gtatctcaat gcttgtgtca gagataatgg attttgtctg
     4441 cttccgtttc ctgcatctga cccttctggt gttacataaa ctgtcgaata tttaggttta
     4501 cggtagtcca ctgtatgaag aatctctttt gtttcataac tagtcatctc tacgtattct
     4561 tctggcggat agtttttatc tggcgtaaaa ctgaaagtaa attcgttcgt tccttccatc
     4621 agttttgtct gataatcaaa ttcccgattc gcagtgatag ccaagttttt tgcgttctta
     4681 agcacctctc ctgttgcttt atttttaatt gtcaataatc cgtcagcatt tgctctaaag
     4741 gacatagtat gatcttggtt tcctgaatga ttactagagg tcacagtgta aatcggatca
     4801 atcaagcgag taggtctttc ttgcccagga ggatctgtcg caggatctga gtaagcaact
     4861 tcgattcctt ctaccttgat cgtcatattc cttgctgcag caaatcctgc ataaataacc
     4921 gattcatctg aagcgtataa tttttcccag tcatacatga tgtcagaagc catttcttct
     4981 cctgtttcag gatcgtaata atatgcctct aaaccagtat tcgttttttt catctctacg
     5041 tccagtttag taatcgagtt cgtggccgga aacagattcc catgaccgtt tccgaatata
     5101 ttataagaac ctgtttcttt ttccaagaac cctgctgaag tctctaaagg tgttgtttcc
     5161 actgagacac tcccaggagc tggcgcagct tgcggatctt cagatgatat tcctgtgatt
     5221 gcccgtgtcc ccaatccaag tcgcatcgta tatttgctgc cgctcgtgtt gaccacttct
     5281 tgtctatccg agtcccagac atattcgatc cgactgccta gtatggcaaa tgaatttgaa
     5341 taaaatgcct gcccaaacaa atgatttttt ggtactgcgt ctcttaccat taaagcaaat
     5401 ccttcttggg cattcgtata ggtccaagat tctatttcga tcgtcgcccg catcctaaag
     5461 ttgatgtctt tggggattgc ttgataataa taggtcattc catctgatcc gcttgactgg
     5521 aattttccgc cgttattcgt ggaagagagc cgcacgccgt cttctaatgg ttcaatggca
     5581 tacctactag cacttgtgct ttctccgaaa acaacccctt tccactcttc atttgcttct
     5641 tcagattgct tttttgtctt ttcattttct gattctttca tcataggatc atttccttct
     5701 tccgtatcca tatcttcact agttcttggt atttcctcca ttgttgacat cccttcaatg
     5761 ttcacgtgtg cattttcttc gttcacagac tgttgttcgt tagcagcaac ttcaacgaca
     5821 gaaattaaaa ccggggtact gagcataagg agattcatca ttagcgcact gtattttata
     5881 cgttttttta acaagtgtat tcctcccgta acaaattaga aagcgtttac ttaaagtatt
     5941 acctaattta tttttactgt caataaaaaa caaaattata ataagtgtat tttataaaaa
     6001 gaaaaaatat aacttaagtt gttgataata aagagattta ttaacgtaaa aagaataagt
     6061 ttttttaaaa taacg
//
LOCUS       DAZAFF010000130         5987 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000130 DAZAFF010000000
VERSION     DAZAFF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.134"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..1150
                     /locus_tag="ACG9BB_002683"
     CDS             125..1150
                     /locus_tag="ACG9BB_002683"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFN2838547.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            1341..2585
                     /gene="glyA"
                     /locus_tag="ACG9BB_002684"
     CDS             1341..2585
                     /gene="glyA"
                     /locus_tag="ACG9BB_002684"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFN2838548.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            2738..3685
                     /locus_tag="ACG9BB_002685"
     CDS             2738..3685
                     /locus_tag="ACG9BB_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFN2838549.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            3819..4448
                     /gene="upp"
                     /locus_tag="ACG9BB_002686"
     CDS             3819..4448
                     /gene="upp"
                     /locus_tag="ACG9BB_002686"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFN2838550.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(4587..4829)
                     /locus_tag="ACG9BB_002687"
     CDS             complement(4587..4829)
                     /locus_tag="ACG9BB_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838551.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            4950..5022
                     /locus_tag="ACG9BB_002688"
     tRNA            4950..5022
                     /locus_tag="ACG9BB_002688"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4983..4985,aa:Thr,seq:cgt)
     gene            complement(5141..5779)
                     /locus_tag="ACG9BB_002689"
     CDS             complement(5141..5779)
                     /locus_tag="ACG9BB_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFN2838552.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(5902..>5987)
                     /locus_tag="ACG9BB_002690"
     CDS             complement(5902..>5987)
                     /locus_tag="ACG9BB_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN2838553.1"
                     /translation="SEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT         2017 a         1052 c         1240 g         1678 t
ORIGIN      
        1 cgactttttg gtttatcaac aagtttatcc actattgaca aatagattat ccacaaacag
       61 tggaaaactt gaggataaac tcagacaaaa taagaatgta tgaaaaaggg gttgtggata
      121 aactgtggaa acgaaacgat atgatgaaac agaattgcaa gaggcagccg aagcactgaa
      181 agaaggagaa cttgttgctt ttcctaccga aacagtatac ggattgggcg ccaacgctct
      241 tttaccaaac gctgtcaaga aagtgttttc tgtaaaaggg agaccgcaag ataatccatt
      301 aatcgtccat gtcgcctctt ttgaacaagt aaaagagtat gtggacaact ttcatccggc
      361 agcagagaag attgtgaaga acttctggcc tggaccatta actttgatat ttaagatcaa
      421 aaaagacact ttgccctcag ttgtgacggg aggattatcc acagctgcat ttcgtatacc
      481 ggataacaaa aaaacactag aagtaatcga actttccggt gttccgcttg tcggtccaag
      541 tgcaaatacg tcgggaaaac caagtccaac gactgcagat catgtatatc atgatctaca
      601 aggaaaaatt accgggatta ttgatgatgg agctacgcga attggtgtag aatccactgt
      661 tcttgattta agtgatccaa cagccatgcc tatgatttta cgaccaggtg cagtaacgaa
      721 agaacaaatc gaagcagtca tcgaatctcc tgttgctatt gatcagcatc ttgtaaaaga
      781 aaatgaaaca ccaaaagccc ctgggatgaa atacaaacac tactcacccg atacacgtgt
      841 tctgatggtc agagatggcg attggtcaac tgcagttcaa tgggcaaaaa ataagaagat
      901 ccgtgcaggt gtcatagcaa gccccgaaat tgcggatcaa gtgcggacag atactgcagc
      961 tgtttatatg tataacgaca actcagtaga agcggcggct aaaggattgt ttgcaggtct
     1021 aagaggatta gatgaaccaa cacttggatt agatttgatt tttgtacaag tgtatccaga
     1081 aactggattg gggaatgctt acatgaatcg cttgaaaaaa gcagcaggac aaaattattt
     1141 tgaaaaatga tcaattggtt attgtcgcat tcttttagta tgatacaatg ctatcaagta
     1201 aatcttctat cagtgaacga aaaactaagc gtaactaatc gtaatagaag agatacgaat
     1261 atttggcaat ttgtttttga tgggaaaaat aaaattgtag gaaaataaaa ttgtaggaaa
     1321 ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat ctttgggctg
     1381 cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc tctgaaaact
     1441 ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa tacgcagaag
     1501 gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt gaaaatttag
     1561 cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag cctcactccg
     1621 gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac acgatcttag
     1681 gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac ttcagtggca
     1741 aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt gattataatg
     1801 tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt gccagtgctt
     1861 acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta ggcgcaaaac
     1921 tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat cctaatccag
     1981 ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc cctcgaggcg
     2041 gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg gtatttcctg
     2101 gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca ttcaaagaag
     2161 cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca aaagcaatgg
     2221 tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca gataatcatc
     2281 tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag agcattttgg
     2341 acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta agtccattca
     2401 aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc aaagaagaag
     2461 atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat gataacgctc
     2521 aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg ttgcataaaa
     2581 aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt ttctgtttat
     2641 tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata tgaggtgtaa
     2701 ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta tctcgttaga
     2761 aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct atgaactagc
     2821 accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga acaaatattc
     2881 cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca atattcgtct
     2941 cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc atggagcagg
     3001 ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg cggttcgtac
     3061 gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat atccaacagc
     3121 aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt caaaaaatct
     3181 ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg ctacagtcat
     3241 gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc tgtattatcc
     3301 agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa actatttttt
     3361 gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag aagagcgtgc
     3421 tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc ttccttcagc
     3481 aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt atgcgcgcaa
     3541 acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca tccatgattt
     3601 tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg cattatctac
     3661 ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt cttttcaaaa
     3721 acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt ttgttttaca
     3781 attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt caagtgatcg
     3841 atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc ggaacaaagg
     3901 tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta tcacgggata
     3961 tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa aaaacgttgt
     4021 ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg gttgacggta
     4081 tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga gatgaagaaa
     4141 cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca cgtcagttgt
     4201 ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc gatgcattga
     4261 aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct gagggagtaa
     4321 aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta gatgaaaaat
     4381 tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt ttgtttggta
     4441 caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct tttttggtca
     4501 tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat aaaaaaacca
     4561 tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta gttaacgatt
     4621 actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc aatctcaaca
     4681 acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc gtgaattgtg
     4741 aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac gatagttgta
     4801 tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa ttataccacg
     4861 ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta caatcagcca
     4921 aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta gagcaacgca
     4981 ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga gaagacagat
     5041 atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga aataaagtat
     5101 gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat aaaacgacta
     5161 gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg atcgagttgc
     5221 ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa aggatgtgtg
     5281 ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat ccaaagatga
     5341 ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata gcgagaaaat
     5401 acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat aataacgggc
     5461 tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt tgcaaaaata
     5521 ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata tttttgacag
     5581 gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca agtgtaaatg
     5641 ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca aatagcaacc
     5701 ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt tctatttttt
     5761 ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag atagagtcag
     5821 tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt gctaaaaatt
     5881 tatcttatat attcattttg attatactac ttttatttgt aaaaattttc tagcaaaacc
     5941 aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagc
//
LOCUS       DAZAFF010000131         5976 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000131 DAZAFF010000000
VERSION     DAZAFF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.135"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..415
                     /locus_tag="ACG9BB_002691"
     CDS             59..415
                     /locus_tag="ACG9BB_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN2838554.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            405..671
                     /locus_tag="ACG9BB_002692"
     CDS             405..671
                     /locus_tag="ACG9BB_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2838555.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            830..1831
                     /locus_tag="ACG9BB_002693"
     CDS             830..1831
                     /locus_tag="ACG9BB_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFN2838556.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(2201..2815)
                     /locus_tag="ACG9BB_002694"
                     /pseudo
     CDS             complement(2201..2815)
                     /locus_tag="ACG9BB_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3055..3300
                     /locus_tag="ACG9BB_002695"
     CDS             3055..3300
                     /locus_tag="ACG9BB_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838557.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            3398..3619
                     /locus_tag="ACG9BB_002696"
     CDS             3398..3619
                     /locus_tag="ACG9BB_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838558.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            3941..4621
                     /locus_tag="ACG9BB_002697"
                     /pseudo
     CDS             3941..4621
                     /locus_tag="ACG9BB_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            4761..5300
                     /locus_tag="ACG9BB_002698"
     CDS             4761..5300
                     /locus_tag="ACG9BB_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838559.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT         2200 a          820 c         1070 g         1886 t
ORIGIN      
        1 aattcgaatt tttatgttat atactataat ataaaatcaa tatatgatat aattgggtat
       61 ggaaaaaata tcttttgaaa catttaaaag acctaatggt catgatgaat ttttagaatg
      121 gttggaaaca ttaccaaaaa aagatagtgc gaagctttta cgtacaatag aagaaacaga
      181 aaagaatggg atgctagtag ctcaacggtt gaaatgggtg aaaaaattag acactaattt
      241 gtacgaatta agaagtaagg taggatcaaa catccaaaga gctatctatt tccatgtggt
      301 caatggaagg tatgtcatta cccatggatt tactaaaaaa acggagaaaa caccaataag
      361 tgaacttaaa catgcaaaag aacttagaga ggagtggttc acaaatgatg attaaaaaat
      421 tgattgaaga aaagcaaaaa gacaaagatt ttgcagaagc atatgaccgt gaaggaagaa
      481 aactcgaaac tgcggttgct ctttatcatg cacgggagga agcaggccta actcaagcgg
      541 agcttgcaca aaaagcacat acaactcaat ctactattgc tcgaattgag agaggggata
      601 acgtatcttt cgataaactt gatgatatcg ctagggcatt aggcaaaaag ctggtagtag
      661 aatttgctta aaagcgagtt gaaaatatac ctgtaatagg ctcctaacta atcaattagt
      721 ttagtgggcc tttttgtact aaatagataa aagttgtcct atagtgtaaa agtcaataaa
      781 ttttatacaa aaattacttt aaagagacat aaagagaaag gtacaataaa tgaaaaccat
      841 aaatctaata aaagtattta tagcatcacc aggtgatata gcagcaaaga ggaatgaagt
      901 agaagaaacc atatatagat ggaatataga aaatacagat aatacaaaag taattttgat
      961 gccaattcga tgggaaacga atgcgattcc tacttatata aataatagct ctgggcaaag
     1021 tgttattaat aaacaaattg tagaaaatag tgatatatta ttagcgattt ttggccataa
     1081 gttgggttct ccagttgata attatagatc gggaactata gcagaaattg attactttta
     1141 taaaaatagc aaaggcgagg taggggtttt tttcttagat gaaccagttc cacaagtttt
     1201 ggctgaagag tatatagaag ttaagaagta cagggactct ttagaagata agggttttta
     1261 tggaacatat agcaaagaca atatcagaag gtttctcaca aaaaaagtga attatttaat
     1321 tgagaaagca caaaataatt tagaatcaaa acgctttgat ttagattcta tttcttacaa
     1381 gacaccttca gtaattaaca aaaaagttac cggaactgat aaagattttg atgacaactt
     1441 agatattttt gatccaatag aatttgacaa tgatgaaaga ttgctgctaa tatatgcgtt
     1501 tatggatgaa aattctatct tttcaactga taattcaact tatagaaaag tagttcaatg
     1561 ggaaagtgat aacaatttaa ggagttactt gagtgataga gtatttgatg tactagaaaa
     1621 attaagtaaa tcgggaattt taaaggaaga atacgattct tcttttgatg agtatccatc
     1681 acgctatatt ttgatttcag aggactataa gaaatttaaa aggtattttt taaaaaatga
     1741 aaaaaagata tctgttatta tggagaaata caaagctcca atacaggctg atccattttc
     1801 caatttggat gaatcagatt taccgtttta aattttaaat agataatttc attttttagt
     1861 agcagaggta taaacttttt taatgtttga attgaaaaga aatgctaata ggtcgacttt
     1921 atgtttctct tgtgcaatcg taaatgcggg ggaccataaa attgatctat tatcgagtgt
     1981 gtataaaata aaattcaaat cttattagtt tacataagat acattatact aagttgtcaa
     2041 tgttaaatgc ctttaaataa ggacgattta tgcatcaatt atatttatgt taactaaatt
     2101 ctttttaaca taaatgtaat caacttagct agatgaatct aaaatgattg tctgtattga
     2161 tgacaaagct aattaataat tacgtgattc aaaaaagaaa tcatacaatt agttggtcga
     2221 tttctttttt tattcggtaa attgtttgcc tagtcacatt gtagtctgtt gcaatctttg
     2281 agatagcaac cccattctca agatcctgaa cgatactcct atagacaagg cgacgttgag
     2341 gatctttggt gtctgcactg tataacttag gacgaccttt atagacgccg tttgctttag
     2401 cgattttaat tccttgtgcc tggcgtcgtt tcgattcagt acgttcttgt tcagcaatca
     2461 tggctgagat ttaaatgatt aagtctttga tgaatttatc taggagagga tttccaattg
     2521 cttcagccat gatcggtaaa ctggtgataa taagtcgcac attcttcttc tttagataat
     2581 tcactgaatc gataatctcg tcataattgc gtccaagtcg atcaatggct tctacaataa
     2641 aaatatcttg ctctctgaca aaatctaaag cttcctggaa aatcggccga tgactaatcg
     2701 tcgctccaga ttgtttttcg ataaaaattt tttcagcgcc aaactttttc atttcttcaa
     2761 tttgacgttg ttcattttga tcgatggatg aaactctaat ataagcaatt ttcaaattta
     2821 ttccctactt ttctataacg tttataatac accctaatca tacgttagaa ctctaaattt
     2881 ttcaatgtta tatttttgtc tatttagggt aaactctaaa tagacaaacg gtttttgaat
     2941 tctaaaagtt aaatagttta taatagaaga aaaagaatgg ggtgacgatt tcttagattg
     3001 aaaaggaaag ataaatatta tagattatta cttttgacag ctgaagcagg atggatggat
     3061 tttccagaag ttgtaaaaga agttcaagag ataggtgcaa ctatttctga tagccgctcc
     3121 tctcctatga actatcagga tgcccatgga attaaaataa ttgatcaaga gagaaatgtc
     3181 agaaaataca caacaataaa tgagtgtgta attaatgaaa atctttgtag ggcaccgata
     3241 acgaaacaca ttaagaatag aagcaaagca aaagacggaa gaacttttac taccttctaa
     3301 gtttttttca tatttgtttg ctatacttag atctaaggaa ttagactttc gctgcccatt
     3361 atttaagttt gaggtagcgt cttgcgaggg atcaataatg gaagaagaaa aaatatttga
     3421 aaaacgctgg gaattggctt cagttgaaca aagagcaagg taccataatc taatgtcctc
     3481 ttaccgtaat attgattgga cttacaaaga aaagaagtac ttgctttggt tatgtcagtt
     3541 agatgtaaat acctttgaaa catttgaagt gattctagat aagattaaaa acagcaatga
     3601 aaaaagagct gatctataat ggttcgctct ttttatttta ttaactcttt ggttactata
     3661 tgtaaaatac agcttcatat tacaataaat ttcaggatac aaatttattt aagcatatat
     3721 aaatataatt gaaataggac ataaatatat gagtatgaga aaggtgattc catgtttaat
     3781 aaatgggcaa accacccaat gaactcgaga ggtagagata atttgaagga gacggacaag
     3841 ttctgggaag aagtatatag taaaaacggt tctgttgcaa agttttccaa aaaatctatt
     3901 ttagtgtaaa attgagaaaa aagacagaga ggacagagta atgaatcatt ttaaaggcaa
     3961 acaattcaaa aaagacgtca ttattgtcgc tgttggttac tacctgcgtt acaatctaag
     4021 ctatcgtgaa gttcaggaat tgttatatga tcgtggaata aatgtttgtc atactacgat
     4081 ttatcgttgg gtataagagt acagcaaagt cctctattat ctttggaaga agaaaaatag
     4141 acaatccttc tattcatgga aaatggacga aacctatatc aaaattaagg gacgttggca
     4201 ttatctttat cgtgcaattg atgcggacgg cttaacctta gatatctggt tacgaaagaa
     4261 acgggatacg caagcagcct atgctttctt aaaacgactc cataaacagt ttggtgagcc
     4321 gaaagcaatt gtgaccgata aagcaccttc tcttggctcc gcctttagaa agttacagag
     4381 tgtgggttta tatactaaga cagagcaccg aactgtgaag tatcttaaca atttaataga
     4441 acaagaccat cgacctatta aacgacgaaa taaattttat caaagtctcc gtacagcctc
     4501 ttccacgatt aagggcatgg agaccattcg aggaatatat aaaaagaacc gaagaaatgg
     4561 aacgctcttc ggcttttcgg tgtctactga aatcaaggta ttaatgggaa taacagccta
     4621 agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca gaaccgagaa
     4681 ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt ttaaagtgga
     4741 aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt atttttactg
     4801 tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa ggtcgtttag
     4861 ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc ctatatgact
     4921 atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa gtaaaagttg
     4981 gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc tatacagtag
     5041 aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga acagttgtga
     5101 aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct attaaaggta
     5161 cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg attctatctt
     5221 tattaattag ttcaataact tttatattct tatttacacg ttatttaata agatgctgcg
     5281 gtgaaatgaa aatagattag atagatatta taaatatgtt gattctattt tcatagcaat
     5341 ggagaaaata cattagcaat atataaaaga aggtatcaag agtcacagtg aatttattcc
     5401 gaaaagtgaa aagagcaaat ataaaattta atgaaaatat agaactaaat aggaaaacgt
     5461 agacgtaatt ttctacgttt tccttttcat atatattaaa tattacggaa aattatataa
     5521 actaatttag tgttttgttt gtaattccta attttgctcc acttttagat aatttttagg
     5581 tctatttaac tcatcagaca ttctatgctc atttttctcc aaatttgttt caactctttc
     5641 gtacttaatt ttgcgtattt gaaaattctt cttttagagt acttttatta cgatactagt
     5701 atgatatata tctatagcaa acagttaaaa tcttcctaaa ataagcgaat agcattccta
     5761 aaacctataa aatttttaga tgaagcgaca agtggacaat taataaacta cttgaacatg
     5821 aaattatgga tcacctttca aatttagaaa atggaattct tatacttatt gcgcaacatt
     5881 tattgattgc aaaattatgt gacaaaaaaa actagtagaa gaaagaataa tggcttgata
     5941 ataaagtact acctattaaa ggtagtactt tattta
//
LOCUS       DAZAFF010000132         5781 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000132 DAZAFF010000000
VERSION     DAZAFF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.136"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..539
                     /locus_tag="ACG9BB_002699"
     CDS             51..539
                     /locus_tag="ACG9BB_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFN2838560.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            835..1116
                     /locus_tag="ACG9BB_002700"
     CDS             835..1116
                     /locus_tag="ACG9BB_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838561.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            1118..1534
                     /locus_tag="ACG9BB_002701"
     CDS             1118..1534
                     /locus_tag="ACG9BB_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838562.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            2202..3302
                     /locus_tag="ACG9BB_002702"
     CDS             2202..3302
                     /locus_tag="ACG9BB_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFN2838563.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSIT
                     SILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYIYTVKKNTNG
                     EYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPLSTEKNGVS
                     SLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMINWRIAETQTSFPWC
                     AAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPK
                     KIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPW
                     YNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            3275..3718
                     /locus_tag="ACG9BB_002703"
     CDS             3275..3718
                     /locus_tag="ACG9BB_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838564.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            4852..>5781
                     /locus_tag="ACG9BB_002704"
     CDS             4852..>5781
                     /locus_tag="ACG9BB_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN2838565.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKE"
BASE COUNT         2157 a          983 c          943 g         1698 t
ORIGIN      
        1 aaaacttact agataatctc caaaacttat ttaaagaata aggggttttt atgacagata
       61 aaactatcaa agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac
      121 tgaggaaatt acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag
      181 aagcagtaac aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg
      241 aattacccga ttcattacct accgattacc tactcaagga gattgaaact aagaataatc
      301 atattgaaga aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa
      361 aattactcga ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc
      421 acgaacaact ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta
      481 ctgaagaaac tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac
      541 ccaacaattt ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat
      601 agcgacatga tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc
      661 ttacgaaagg tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca
      721 ctttttcgtc attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat
      781 actatatttg tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac
      841 attttagttc ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa
      901 ctcacaacaa aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa
      961 aacgattttg accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa
     1021 aaatttatcg atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac
     1081 aaccaactca ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa
     1141 acacgttacc ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc
     1201 agatatcaat aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac
     1261 taatacgaca actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat
     1321 tatgtccgaa atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat
     1381 cggtacaaat tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc
     1441 ctcccgacga aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag
     1501 aaattttgac ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt
     1561 tctcagtttc ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg
     1621 cacaaaaaaa gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc
     1681 caatctccaa atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg
     1741 aaataaaaaa agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga
     1801 aaaagcagcg cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa
     1861 gtttctaaaa aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa
     1921 tcaagcttcc gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcggctgg
     1981 atcccttggt acaggcgtta cttggtagta tacacattat gttaactaac gctataaata
     2041 acgattatgt tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt
     2101 gtatatttta attataggtg tataccactt aaatttaaca caaaggcgtg attattttga
     2161 aacccaaaaa gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta
     2221 taggaattac aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa
     2281 ttgaagaaca agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc
     2341 ttttaatgga aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa
     2401 tgctcgatgg agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg
     2461 ataatacgat taaatatatt tataccgtca aaaaaaatac aaatggagaa tattcaggaa
     2521 gtatttcaca atttttagca aaagaacttc aaaatctagt agatagtaat ataacagata
     2581 caaccttatt ttttcaagat aatagcattt attattccga taatggacaa attaaactac
     2641 tttggaaatc acctttaagt acagaaaaaa atggtgtttc ttcccttaca gaaaaaaatg
     2701 atcttatatc cgtaaatata aaaaataaaa ttaaaaattt ttctaaaaaa caaaattttc
     2761 ctgcggttaa acgtgtaaaa agtgtctcct ccggagataa ctttattatg attaattggc
     2821 gtatagcaga aacccaaaca agttttccgt ggtgcgctgc ttacgtagaa gctgctatct
     2881 tatgtaacaa aacagatgta cgtgctactt cagctagagc cattatgcat tatacctatc
     2941 ctaatttaaa caacagtcaa attgaatcaa aaagtcttaa tcaagatcaa ataataaaat
     3001 atgcaaatta ctgtggtgtt tatcctaaaa aaatagcacg aatactaaac ctttcagagg
     3061 tccaaacaca actaagaaaa ggaaatgcca tttatatggg aactgctggt tatggaaatt
     3121 atagtaacag ccgtcatgca cttgcattat ttgggtgggt caatcaaggt ggttatcaaa
     3181 cttactatgt gtggaatcct tggtataact acccaactgt aataagtgca aatgcaggac
     3241 cagttacaat tcctgtacct ggaggtggtt ttacttggga caataccatt acaaattggt
     3301 aaataaactc attgctttag ttatgggtct agtgttagtt cttttcttaa ctagttgttc
     3361 aaaaaaacaa gaaatacaaa agactgatct gattcctttt ctaggaactt ggcaaactaa
     3421 agatcattca gaacgtctag taatcactaa tttaaatact tcaaaaaata tagaacaata
     3481 taccatctta gtaaaagaga acaatcataa aattcaaatc attgataaaa atttaaatat
     3541 aaatgaacaa gttttcattt acaaagcaac aaataattta ctaacactgc gctcgaataa
     3601 aaaacaatta gattacactt ttagtttgga ttctccaaaa aagctcgtat ttattttgac
     3661 aacgaatgat gggacagaag ggacagctaa accaattgaa ttttttaaag ctgattgatg
     3721 aatagttgtt tgatattaca aaacaataat ttcaccgaag gtgaaacaag cgaaacttta
     3781 caaaaagtgg ctagcatttg ctagcctttt ttgtattgtg gagcgtgggt tttgaggggg
     3841 attcaagggg gagcttgcgc ccctttgaca agcggttatt tgcaccaaat acgtaggtat
     3901 ttggctagca aatatagagc ttgccaagcc ctttagtgtg ttttgtagaa agggacctta
     3961 tagtcacttt cacattacaa acgatacgtc cataggacgt atcaaaaggg tgggacttat
     4021 gagcaaatta aagcaaaatt tggctagcaa tccttctaaa aaatacacct attgatcaga
     4081 gtcaaaataa atcagttttc gagtgagcga atccaaattt gcaactctga agctttgcaa
     4141 atgagtgtgc cggcttccgt aaaagtctag gtaacattag tcgatatata catagggctc
     4201 taatttgcgt tttaaggcgt tttaaacgtt ctggtatgta attataccaa agcgacttta
     4261 aaacgcctta aaacgaattt tagaggttct gtagaacgtt ttagggttgt tagggcttgc
     4321 cctgaccgcc tgtaaggcgc tttaaaaaca agataaggga ttttatctag acttgtctta
     4381 aaacgccgta gatgagcgct cagagcttct aaaaggactc cttacgaact gtttttaagc
     4441 aaaggagcct ttaggcgact gcgcagttta atgtgagagc gtagcgatca cacctttaaa
     4501 gcgaccgtag ggagcagatt cccagtttag gaaatttcgc gaacctgttt ggagaaaaaa
     4561 agtaggaaaa ttttaggcgg gaaattttgc ccccctcttt tataatctct tttataacct
     4621 ctttttaaac ctctttaccg tcggctgtat ccttactctc ccaaggtgtg cagaatcttt
     4681 taggggtctt tttgtctgtt tttaggggtc tttttgtctg tttttagggg tctttttgtc
     4741 tgtttttagg ggtctttttg tctgttttta ggggtcttta attagggggt tgacccccat
     4801 tagaaagctc tttataattt tgttaagtag ttaatcttga aggaggattt tatggcaaat
     4861 gaaattgtga aataccacca tgaattaaat acgattcctt tacgcaaatt tacgtcagta
     4921 gaaatgaatt tattcttctc tataatatct agaatgcgtg atgtaggcga taaaaaagta
     4981 caatttacat tcgaacaatt aaaagattta agcaattata aagcaactgc aaatgttcga
     5041 ttcatagatg atttagaaac aacgtatgac aaattaatgg atttacgttt tggtagaaga
     5101 agtgctgacg gtttgcaacg tgaacgattt gttttattta atcaatttaa aatcgatgga
     5161 aaagctgata ttccttttgc tgaaatacaa gtccatgaga aagccttacc cttacttaat
     5221 aatcttgaag aatgggttcg atattctctc caacaattca atgaattaga aagcagttat
     5281 tccaaaacca tgtttagatt attaaaacaa tacagaacca aaggaattgc caatttttca
     5341 aaagaagact ttcatgaatt acttgatatc cctaaaagtt atagagaaag tgatataaat
     5401 aaaaaaatta taaagcctat tcgccaagaa ctaaccgcaa tcttcaaagg tttgacaatc
     5461 caaaagagat acggaaaagg acggggaaag cctgtcattg gctatcaatt tacatttaaa
     5521 cctgagataa aaaaatcaga cgactttaac aaacatgctc caaccaaaga agaacaaaat
     5581 aaagctttgg aactagctaa tcaacaggtt caatctgaaa aagaacctac ttcattacaa
     5641 gaaaaaatca aagaacaaaa acgaaaatta gaacttttac aacagttagc agaattagaa
     5701 agaaaacaaa ataccccaag taagaaaatt aacaaacaaa tagcagataa caattccgat
     5761 attaccccag aatttaaaga a
//
LOCUS       DAZAFF010000133         5734 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000133 DAZAFF010000000
VERSION     DAZAFF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.137"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="ACG9BB_002705"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="ACG9BB_002705"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFN2838566.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="ACG9BB_002706"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="ACG9BB_002706"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFN2838567.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="ACG9BB_002707"
     CDS             2591..3376
                     /locus_tag="ACG9BB_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN2838568.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="ACG9BB_002708"
     CDS             3389..4240
                     /locus_tag="ACG9BB_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFN2838569.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="ACG9BB_002709"
     CDS             complement(4364..4816)
                     /locus_tag="ACG9BB_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2838570.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="ACG9BB_002710"
     CDS             complement(5281..5649)
                     /locus_tag="ACG9BB_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFN2838571.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT         1935 a          929 c         1161 g         1709 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taat
//
LOCUS       DAZAFF010000134         5727 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000134 DAZAFF010000000
VERSION     DAZAFF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.138"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACG9BB_002711"
     CDS             196..921
                     /locus_tag="ACG9BB_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838572.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACG9BB_002712"
     CDS             911..1420
                     /locus_tag="ACG9BB_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838573.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACG9BB_002713"
     CDS             complement(1490..2938)
                     /locus_tag="ACG9BB_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN2838574.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACG9BB_002714"
     CDS             complement(2938..3654)
                     /locus_tag="ACG9BB_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFN2838575.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACG9BB_002715"
     CDS             complement(3635..3985)
                     /locus_tag="ACG9BB_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFN2838576.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACG9BB_002716"
     CDS             4129..4902
                     /locus_tag="ACG9BB_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFN2838577.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG9BB_002717"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG9BB_002717"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1123 c          994 g         1948 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tttttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DAZAFF010000135         5589 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000135 DAZAFF010000000
VERSION     DAZAFF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.142"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG9BB_002718"
     CDS             complement(<1..300)
                     /locus_tag="ACG9BB_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFN2838578.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG9BB_002719"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG9BB_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG9BB_002720"
     CDS             complement(1014..1304)
                     /locus_tag="ACG9BB_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838579.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACG9BB_002721"
     CDS             1706..1942
                     /locus_tag="ACG9BB_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838580.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACG9BB_002722"
     CDS             1949..3901
                     /locus_tag="ACG9BB_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFN2838581.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG9BB_002723"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACG9BB_002723"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFN2838582.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="ACG9BB_002724"
     CDS             complement(4797..5489)
                     /locus_tag="ACG9BB_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN2838583.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1821 a          972 c          903 g         1893 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttccagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaa
//
LOCUS       DAZAFF010000136         5457 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000136 DAZAFF010000000
VERSION     DAZAFF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.143"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(534..1100)
                     /locus_tag="ACG9BB_002725"
     CDS             complement(534..1100)
                     /locus_tag="ACG9BB_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFN2838584.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1100..2056)
                     /locus_tag="ACG9BB_002726"
     CDS             complement(1100..2056)
                     /locus_tag="ACG9BB_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFN2838585.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2056..2712)
                     /locus_tag="ACG9BB_002727"
     CDS             complement(2056..2712)
                     /locus_tag="ACG9BB_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFN2838586.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3082..5100
                     /locus_tag="ACG9BB_002728"
     CDS             3082..5100
                     /locus_tag="ACG9BB_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN2838587.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5170..5376
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1796 a         1052 c         1043 g         1566 t
ORIGIN      
        1 attgaaacat ccatctggaa tttatctttt ttactgcttt gatcataaaa gtctgcatat
       61 tcatcactga tattttttct aataaattct tccatcataa aaatcacctc aaaacgatta
      121 tacgaactta cgttcttgtt gtaaagcgat atttgagggg caaaaaaggg gcataatttg
      181 actgatatta agttagctat tgatatatga atgacataca aaaatataaa atcttttaaa
      241 aatgcttata tatcaatgtt tttgccattc ttttatatat ctgttatatg tcaaaaaaat
      301 gtgttcaatt tcaatttgta tttgaataaa gaggttgtga aaaagctgtc ctgcatcaag
      361 taataagaac agccaatcaa aaacagcttc tcatgttttt gatttggttg gacttattac
      421 cgcagatata agcttttgag caccgtgaat aaagaggtta tgaaatcaac gctctaacct
      481 gagtattaga gaaaaaaacg caaaacatat catttatgtt ttgcgttttt tatttatctt
      541 tttttctcaa tacaatataa aataggcggc tggttctttt ggttgataaa ccgataattc
      601 agcacattat atgtttcctg tggcagtttg gaacaaaaag aatcaaccat atccagttct
      661 ttttctccac cttcatgacc atagtagacg acaaggatca atcgtccgcg aggcacaagt
      721 cgtttcaata tttcttccat agctgtgcgt gtagtctctg gcatagtgat gattgcttta
      781 tcactttttg ggagataccc tagattgaaa atccctgctt tgagattttg ttgttcagct
      841 atcacttctc ctaacgtttc gtggccttgt agaaacaatg agacacgttc ttctaactga
      901 cgctgttcta gcctttcacg agtagcatcg attgcttgtt gctggatatc aaaactatag
      961 acatgaccag tttttccgat gtgttctgct agaaaaaccg tatcgtggcc attgcccatc
     1021 gttgcatcta cgacgtgatc ccctggttgc aagatttctt gcagtaatgt atggctgaaa
     1081 tgcaaagcag tttgcaacat cagacgctca cctttttccg tacatcatat tttccttgga
     1141 agctatctct gcgaagaagt tcttcatcaa ttgcattcaa gacttcccat tttttcaagc
     1201 tccacattgg tccaatcaag gaatcccacg gcgcatcccc tgtcaaacga tggatgatga
     1261 tctcttttgg tatcatttcc agctggtcgc aaatgacatt gacatagtca ggacggctca
     1321 ttaattgaag gcgaccttca tgatagtccc gcaacattct cgttttgcgc atcagatgca
     1381 tcaaatggag cttgattcct tggatatctg aatctaaaat ggtgcgtctg acattttctt
     1441 tcatcatttc taaactttcg cctggtaatc cgttgatcaa atgcgtacaa acacggatat
     1501 tatgtttgcg taatttagcc acgccatcta aataagtttg ataatcatgt gctcggttga
     1561 tcagttttga agtttcttca aaagttgttt gcaatccaag ttctacccat aaatacatcc
     1621 gctcgttcag ttcagcaagg taatcaacta cttcatctgg cagacaatcc ggtcgtgtac
     1681 cgactgataa tcctacgaca cctggtaaat tgactacttg ctcaaatcgt tctttgatta
     1741 tttcaactgg cgcatgggtg tttgtaaaat tttggaaata aacgatgtat tgttggacat
     1801 gaggccattt ttgatgcatc atgtcgattt ctttctggaa ttggattgga agagggtctt
     1861 caggtgcaac gatcatgtct cccgaaccag atacactgca aaaagtacaa ccgccatggg
     1921 caacagtccc atctcgattc ggacagtcaa atcctccatc gatcggcacc ttgaaaattt
     1981 tcccgccaaa ctcatttcgt aaagcgtaat tccaagaatg atatctttta tttccttctg
     2041 aataaggaaa agtcattaaa agtccttctt tctgtaagag ttatctattt gttttctttt
     2101 ttctctatac actggataag tcaggcacag ccaagaaaga ctgagtaagt aaccgccgat
     2161 gatatcagta ggaaaatgaa cgcccagata aatgcggctt atcccgacac ttactataat
     2221 aaacatcatc agcaattgaa gcaatgtgcg taaggttctt ttttgaacaa aagctggaag
     2281 cagaaatagt aaagaaccgt aaagaatcat actgcctgct gcatgtccac tagggaaact
     2341 gtaacttgtt tcaactacta agtgcgccaa tgaaggacgc tctcgcatga aaaatagttt
     2401 gatcaccggg ttgatgattc cagcaattac aataaatcca gaaacgaacc aaaatgtttc
     2461 tgcccttttt ttcttatacc acatccataa agccgcacaa gcaaataaaa tgatgacggt
     2521 aagaggatta ccgaatttag tcataaagag ttggtagctg ttcagatcag gataagaagc
     2581 tctgacaatg cctgtaaacc actgatcgaa accagaaagc cacgacagat ggaactttac
     2641 gatccagccc aaaatgaaaa acaggacaag aaagaagcta ccagccacct gaaatgatgt
     2701 tttatatttc atacaattcc tcctttgaag caatgactta ttatagcatt tttggtccag
     2761 cgaaaaaaac ggtaaagaga taaaaccaat gtatgattcc tctttgaata accaatattc
     2821 ttataaaaaa aataactgta atcggaaaga cattttttgg taatgcaaat gaaatgttag
     2881 ggaaataaaa tcagtgtttt tcgaaatcct tttaaacaag aacgtagacg taggaagaac
     2941 ttgaaaatga acagaagttg ttcattacgg taaattacaa tatgattaca aaaaacaaca
     3001 aagctcttaa ataactttta ataaatatta agagaagtta taattattaa taatagataa
     3061 attgaagttg gaggatagaa gatggaaaac attgcaagaa aagaacggag acgtttagaa
     3121 cagaccaaac gttttcgaaa agcaaaacga ggggcagcaa tcgtgggaac tgcgatggta
     3181 ggctgttctg tcgctgcccc attagtacag ccggttcctg ttcaagcgga tgaaacacct
     3241 gttcagttcg gcgcaagaat caatacagcg gcatttatca cagaaattgc tacttatgct
     3301 caaccaatcg ctcaagccaa cgatctatat gcatcagtga tgatcgcaca agcagttgta
     3361 gaaagcggtt ggggcagtag tgcgttatct caagcacctt attataatct ttttgggata
     3421 aaaggcagct atcaaggaca aaccgtctac atggatacgc ttgaattttt aaataatcaa
     3481 tgggtaaata aaaaggagcc tttccgacaa tatccttctt ttgctgaatc tttcaatgac
     3541 aatgcctatg tacttagaaa tacttcattt ggcaacggtt attactatgc cggtacttgg
     3601 aagagcaaca cgaaatccta tacagatgcg acagcttgtt tgacagggcg ttatgcgact
     3661 gatcctggtt acgcaggcaa attaaataat atcatcgcaa cttacggatt gacccaatat
     3721 gatacaccag ctagcggaaa cgcaggcggt ggagcgacta caggtggcaa taatactgga
     3781 agtgcaggta gtactggcgg ttcaagcaat tctggtaata caaactcagg ttcagcaact
     3841 tcgaccactt atactgtcca atctggtgat tctgtttggg gaatctcaaa taaattcggt
     3901 atcacaatgg atcaattgat ccaatggaac aacatccaaa acaatttcat ttatccagga
     3961 caaaaactga caatcaaagg tagccaggaa aatggctctt cgacgaataa ttccggtaac
     4021 aataccaatt cttcaggaaa tgctggtaca tcaaacggag gacaaacaac tggtgcgaaa
     4081 tacactgttc aatctggtga ttctgtttgg aagatctcaa atgatcacgg aattacgatg
     4141 gctcaattga ttgaatggaa caatatcaag aacaatttcg tttatccagg acaacagcta
     4201 atcgtatcaa atggcggttc ttctaatgct ggagcaaata ctggctcaaa tactgggaat
     4261 acaagtggct ctagttcgtc cagctcaagt tcttctaata cagcttcagg aacgaaatat
     4321 acagttaaag ccggagactc tgtttggagc gtctcaaata aatacggaat tacgatggct
     4381 cagctaatcc aatggaacaa tatccaaaat aactttatct acccaggaca acaactgact
     4441 gtttcaaaca gtggatcaac aaacagcacg tcaggtaata caggttcgtc aaccaataca
     4501 aataacagca gttcagttaa tactggcagc agttcgtctc aaggcactta taccgtgaaa
     4561 gcaggtgaat ctgtttggag cgtggcaaat aaaaacggca tcacgatgga tcagttaatc
     4621 gaatggaata atatcaaaaa caacttcatt tatccaggac agcaattgat cgttaaaggt
     4681 ggaacttcgg caaatacaaa tacaggttca actacttctg cagcgaaacc aaatacaccg
     4741 aatacgtctg ctacgacaag tacatcatcc actggtaata cgatgtatac cgtgaaagcg
     4801 ggcgagtcag tttggagcgt agcgaacaaa catcatatca cgatggatca gttaatcgaa
     4861 tggaataata tcaaaaataa tttcatctat ccaggacagc aagtgattgt gaagaaaggt
     4921 tcatctcaat caacaaacca acaatcagtt actggttcga aaacctatac tgtgaaatct
     4981 ggagaatctg tttggagtgt tgctgacagt catggaatta caatggctca actaatcgaa
     5041 tggaacaata ttaagaacaa ctttatttat ccaggtcaaa cgttgattgt agctaaataa
     5101 aatagctgtt cgaagaaata acgattgaat tccacaaaaa tatttgttac tataaggcta
     5161 cttaaatatg acaacacttg aaaaggaagt agtaaaaaga ctgaagtttc agagagcgca
     5221 tggctggtga gaatgtgcac ggatgctttt gaactcgcct ttgagttgcc cttgctgaat
     5281 acccgagtaa gcaagcggcg gtgacggccg ttacaacgtt cgaggccttt tcactaaggt
     5341 aaaattaggt ggtaccgcgt agattacgtc ctataaaaag agcaatcttt ttataggatt
     5401 tttttatttc ttttgtgaag tttgagaaaa caaagcaaaa ggaaataaaa ataaaca
//
LOCUS       DAZAFF010000137         5451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000137 DAZAFF010000000
VERSION     DAZAFF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.144"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1556)
                     /locus_tag="ACG9BB_002729"
     CDS             complement(51..1556)
                     /locus_tag="ACG9BB_002729"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFN2838588.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1648..2238)
                     /locus_tag="ACG9BB_002730"
     CDS             complement(1648..2238)
                     /locus_tag="ACG9BB_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFN2838589.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2417..4063
                     /locus_tag="ACG9BB_002731"
     CDS             2417..4063
                     /locus_tag="ACG9BB_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN2838590.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4112..4831
                     /locus_tag="ACG9BB_002732"
     CDS             4112..4831
                     /locus_tag="ACG9BB_002732"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFN2838591.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5310..>5451
                     /locus_tag="ACG9BB_002733"
     CDS             5310..>5451
                     /locus_tag="ACG9BB_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFN2838592.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFK"
BASE COUNT         1646 a          996 c         1100 g         1709 t
ORIGIN      
        1 acagtccctt tttttcttag aaatgggaaa ccactagtat ataaatacca ttattcttca
       61 atcaaccgct tcacgattgc ataatcccct tcataaggtt catctacacc attgattttt
      121 tgatagggat ccacgccttt tcccgcaata acgactgaat cttgcggagc gctttctgct
      181 aaagcgcgtc gaatcgcttc aggacgatcc atttcataat gaacgatcaa atctggatta
      241 gtaattgctt cattgatttc ttccgcgatt tttttcggat cttcaaatgc tgggtcatct
      301 gccgtcaaaa aggcgatgtc agctgtttct gatagcactt ttccaaaatc atgtcgacgg
      361 gagattgctt tgtttcctgg gctgcctaat acaacaatta cacggccatc tggatgttca
      421 tttttggcaa attccagcaa tgttttcaag cttagataat tatgtgcata gtccacatag
      481 acatgcgctc cattggattg gatcaattga tccattcggc ctggtacacg agcttctttt
      541 aatccgtctt gtgcatcttg actggaggca cctgttagtg ctgcggcaat gatcgcactg
      601 gctgcatttt cttggttgaa tcctccagcc agcaaaatat cataagcagt atcgcagata
      661 gctaagcgat cttcatgaga agataaagta aaaccgtgag tacctttatc tgaacggatc
      721 acttgataat tgctttcttc cgaacgacca taagtgatcg ttggaatctg gaatagatcg
      781 gctgtttccc taagtaaatc aaaatagtca ctttcatgat ttaaaacaac tgtttttgaa
      841 ttcaagatca attgtcgctt gcaatagaaa tagtcatcaa atgtcggatg ttcgatcgga
      901 ctgatatggt caggcgagat attcaagaag attccgacat ctaaagtcaa gccgtatacg
      961 cgttgtgttt tgtaagcttg tgaagaaact tccatcacta gatgagtcat accattttca
     1021 actgcttctg acatcatacg atataaatct aatgactcag gcgttgtcaa atgggatttg
     1081 aaatatgttt tgccatctaa cgttgtattc atagtagaaa gcaatgctgt ttttttattt
     1141 gttgtgtgat ccaagattgc ttttgtaaag tacgcagcag ttgttttacc ttttgttcca
     1201 gtgaatccga tcactttcaa tttattttgc ggatagtcgt aaaaagccat agcaagaagc
     1261 gccattgctt tacggatatc cgttacgatg attcctactg ctgaattttc gtatggttgt
     1321 tctgatacat aatattgaat accctgttcg attgctgaat ccaaatattc ttctttgaaa
     1381 ttcattcctt tacagaaaaa caaagtagaa gcatcagctt ttcttgaatc ataacttaac
     1441 tgaagaaatt cagctttttc tggtacatca agatgccagc ctcttgttga aacaaattct
     1501 tttaatagat tttcttttaa tagtaaatga cggatgtctt ctaaagagaa gctcattgat
     1561 tgatccctcg ctttattcaa tttcttggtt tattatagca cactcttttt aatatatcgt
     1621 ttgtttccgc tatgacaaaa tccagcttta ttcaaatttt cttgacacag gggacaagcg
     1681 gatgagtatt ttcataaaca atgaactgac tgtaatagat aggagaacga atagtgtctc
     1741 caacaatcca aaaacataat aattcaaaaa gacgataaga ctattggtaa gtaaaagtcc
     1801 ttgcggcatt ggaagccgaa aaaatttgtg gaaaataatc ggcggtatcg ttataccgcc
     1861 atttgaggcc ccgactgtgt acaatgtgta aaccccaaga ctgaacaaaa gacttcctga
     1921 aatcagtgaa aagaaatgtg aggagatcat ttccactttt ggaacaatct ctaaaacaac
     1981 aggaaaaagc agacttcccg ctacagtctt aaaaaatact gattttccta ataggacagc
     2041 ggctagaagc agcatcataa gattgatgct ccaaacagta actgagcgtt ggatagaaaa
     2101 ggccgtttca actaaatgtc cgatggcgcc cactccggcg gaagctatat cgtgagggag
     2161 caagaaccaa ttcaatccaa ctgtgataaa gaaaagacct aaaaatataa cgggtagttt
     2221 ttttatttct tgtgtcattg atatctccta taaatgttag ccataaaagg ctgtatattt
     2281 ctttacttta ccaaaaaaac gaaaccgaat tcaaaaaaac aattgttctt ataaaagtta
     2341 tataaaatcg attgccagtt tctgttttac caattaaaat gatagtaatg ttttgtagaa
     2401 agttggaatc tgaccgatgg ataatcaaag aaaaccagca ttagatcaat taacaccgca
     2461 agaaaaaatg gcacgaggct ctgcttggat gaccgccagt aatatcattt caagattgct
     2521 aggcgctgtc tatatcattc cctggtatgc ttggatgggc gaaaatgcta aggctgccaa
     2581 tggattattc aatatgggat acaatatata tgcattgttt ctactgatct ctacagcagg
     2641 aatacctgct gcgattgcaa aacaaactgc acgctataat tcattgaatg aatatggcac
     2701 ttcacgacga ttatttatcc gtgcattaca gatgatgggc gggctaggcc tgttgtttgc
     2761 tttgttcatg tacatcgcct ctccttggct agctcatgct tctggtggcg gagaagaact
     2821 gattccaacg atgcgttctt tgagtatcgc tgtattggtt tttccttgta tgagtgtgat
     2881 acgagggtat tttcaaggca atcaagaaat gatgccttat gcgctgtcgc agattgtaga
     2941 acaggtggca cgtatttttt atatgctatt ggctacattc atcatcatga aagtcatgga
     3001 tggtgactat gtgacggcgg tcacgcaatc aactttcgcg gcatttatcg gtatgctagc
     3061 aagtattctt gtccttttgt attttttgaa gaaacatcaa gcgtatacat cggcgtttat
     3121 tcactacagc gaaaacaaaa taaatattgc aacgaaggaa ttgttgttag ataccgttaa
     3181 agaagcgatt ccgtttatta ttgtgggttc aggggtaacg atcttcaaat tagtcgatca
     3241 gttcactttt atgaagatca tgtctgcatc caccgagtat tccaatgcac agttactgga
     3301 tcttttttcg atttttagtg ccaatccaga taagttgacg atggtagtta ttgcattggc
     3361 aacatcgatc gcgtctactg ggttaccgtt gattacagaa gctgttacgt tgaaagatcg
     3421 agtaggtctg gcaaaattga caagcggaaa tttacagttg ttttcatttt ttatgtttcc
     3481 ggcgacattt ggtgttatgt tacttgccta tccgctaaat acattatttt atacaccgga
     3541 ttctctagga agtaatgtac tgatccaagc aagttttgta ggactcttcc taggactata
     3601 tatgctagtt tccaacatgc tgcaaggaat gtttgaaaac aaggcggcaa ttaattattt
     3661 ggttattggg tttctagtga aactccttct tcaataccca gccattcgtt tatttgaagt
     3721 atacggccct ttattggcga caatgatcgg atttgctgtg tcttgtacct tgatcttaaa
     3781 gagaatccat gaagtagccc gctttaaacc aagttttgtc tggcgcagga cattgctgat
     3841 ctttattctt accttgatta tgcttttggc ttcgtggatc acaaagatga tttttggtgc
     3901 ttttttaaac gaagatagca aattccagtc tctattgttg attcttgtag tagcaggtat
     3961 aggggcttta gtgtatggtt atttagcttt gaaaatccgc ttggcagaaa gactactagg
     4021 accaggaata gcgcgtttac gacataaatt aaaaataaaa taagtgaatc agaagagcta
     4081 gattgatgct cttttgaaat gggggaaacg catgcgttta gataagtttt tagcagatat
     4141 gggacttggc agccgcaaag aagtcaaagg attgatcaaa aaaggaacga tcgaagtcaa
     4201 tggacaagtg gtgaaaagcg ataaagcgca agtaaatgaa ttcgaagatg cggttactta
     4261 tttaggagaa atcattacgt atcaaaaaga tttttactac attttgcata aacctgcagg
     4321 agtcatttct gctactcagg ataatcatga tcaaaccgta atcgatttat tggaagacca
     4381 ggattatcgg gaagatcttt ttccagtcgg ccgattagat aaagacacag agggcctctt
     4441 gattttaacg aatgacgggg tgtttgccca tcagttacta tcacctaaaa aacatgtgga
     4501 gaaagaatat ctagctgagg ttagcggtat aatgacagaa gaagatatcc gtttatttgc
     4561 agaaggactg attattgatg gcgatgagca gacattgccg gcaaaactcc aaattgatca
     4621 agtagatcaa gagacagaaa cttcaattat ccgtctgatt ttgcatgaag gaaagtttca
     4681 ccaagttaag cgaatggtca aagcagtagg aaaagaagtc ttatatttaa aaagaatccg
     4741 tatgggaaaa ttttggctgc ctaaagattt gaataaaggg caatatcgac ccatgaccaa
     4801 agaagagcta gaacaagtga gagaaaaata attgaatgga tagctaaaag aataagcaga
     4861 gtagttgtag cgccatctga aaagcagact tgtccgtctg actttcggat ggcttttttg
     4921 tgaaatttcg gcttatttcc gtagaagcta cttctggaac accgtttgtt cggaataagg
     4981 gactgtgaca agacttttgt tacagtcccc attgttcctt gtgactcaga gttagatagt
     5041 aaggacacga tctccctaat actcaggcca aaacgctgaa ttcacaacct ctgataaacg
     5101 gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
     5161 ttcccgtttt tttctctaat actcaggcca aaacgctgat ttcacaacct ctcctatact
     5221 ttgtttcatt ttccctatac tttgtttatt tattctcatt attgaaagcg ctataattac
     5281 gttgcgaatg aatcgaaagg ggtttttaga tgaaaaaacg aaaggggttc aatcattttt
     5341 ttatgaatct atggcgttat aaagcacttg tcctgatggc tgtaccgggt atgctttgga
     5401 tgattttttt cttttacata cctgtaatgg cgaatgtcgt agcattcaaa a
//
LOCUS       DAZAFF010000138         5341 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000138 DAZAFF010000000
VERSION     DAZAFF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.145"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACG9BB_002734"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACG9BB_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACG9BB_002735"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACG9BB_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2090..3130
                     /locus_tag="ACG9BB_002736"
     CDS             2090..3130
                     /locus_tag="ACG9BB_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFN2838593.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3954..4286
                     /gene="traE"
                     /locus_tag="ACG9BB_002737"
     CDS             3954..4286
                     /gene="traE"
                     /locus_tag="ACG9BB_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFN2838594.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4905..5246
                     /locus_tag="ACG9BB_002738"
     CDS             4905..5246
                     /locus_tag="ACG9BB_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFN2838595.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1942 a          728 c          900 g         1771 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta attttaatgg
     1621 tagtacataa ttattatata tatctgacat tggtagtact tattgtattt tttagtagta
     1681 tataatctat tgttttctag cctaaagcct tgtcttataa gaataatata atatatacta
     1741 ctgaaataag gtagtatata ttactagtaa tggtagtatg ttttatatgc ataaggtagt
     1801 tcttaattta ttttttgttt tgacactggt agtacttaat tatctttaat tttaaaacta
     1861 aattttattc atacgttttt ctggtagtat ttaattataa ataatattta tttaaagtag
     1921 ttcttaatga ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt
     1981 aattaaaaaa cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag
     2041 agtaggtaat tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt
     2101 taaatttttt aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt
     2161 tattgtttct gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt
     2221 aaaagcaaga ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta
     2281 ttttacttat acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat
     2341 agcaatcaaa aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa
     2401 caaacagtta gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga
     2461 aaatgatatc tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc
     2521 agagggtatg aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc
     2581 tcaagagggt atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat
     2641 cataccagaa cttaataata atatattaga cactaataga cacaatatag acactgaaaa
     2701 agaccgccta caagatcaat tgttgttaga caattttgag acaattatga caaacgacag
     2761 cattgctacg tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta
     2821 cagtgaagct caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga
     2881 aagtgatatt tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga
     2941 attatacacg acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat
     3001 ccagaacctg atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa
     3061 agtatcaagt gaaaaacgtt tgaattatgc aagctcccca agcactatta cgaaagactg
     3121 gttagagtga aactttactg acaaagtgat tgttgcttca tggagtaaca ttaaaattaa
     3181 aaatttttta ttattctaat tatattacga atattacaaa ataatagcta ttttaataga
     3241 atttataagt taaaatatta tgataaaaat cacaaacgta gccttttacg tcataagtgt
     3301 taaaagaaat aattaatttc aaatatagaa aagggaacgt atgtttgcat actccttttt
     3361 tttgagttat aataatacgc gaagagagat aatttcatta tttaaacaag ctaaaaatct
     3421 tgatattact tcttttcagg caatacgaat attattcgct tacatttttt gtataaattt
     3481 aattgtctgc tatgcttgtg atgaattgat tttcgttttg cacttttgga tgataaattt
     3541 ggacaattga aaatacaaaa aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc
     3601 gtcagcgttc atacacggca ttaagatttg gcccttaatg cgttaacctt tcagtgttac
     3661 ggtattaccc gcaatggatt tagtataaca gatattcaaa agtgatacca ctttttttgg
     3721 ctgtgttaag gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat
     3781 aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt
     3841 ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa
     3901 aataacaaga aaaacttgtt aaaaatattt aaagaaattg ggtggtttat tttatgtcat
     3961 atacaggaat tttgagtctc aaagatattt gtcactacgg taaacggtgt accgctacag
     4021 agaaaatcac gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat
     4081 atataatcca gaaagacaaa gtctcagagg aaatgattta ttacattggg aaacaaaagc
     4141 aaataatttt aaaagatcct atccccttaa aagagctata tccaacaatc aaacatgttt
     4201 atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa
     4261 aagggatggg gcgtttgatc ggctgaattg atcattctca taacttgtat tttatcgttt
     4321 ttcgcttgct tgaaaatatt gtcgagactt ctaaaggaac ttagagtcac tagaaaaata
     4381 aggagtagac ttgatctatc aacaattccc ttcggggagt gacggggtag gttttaacga
     4441 ttggtaacgt aaaaaattgg tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg
     4501 aatttgtgta aataataagc gcagagtaaa caagagggaa tttagaagtt agacaagtta
     4561 gaatagcgtc tagattaatc atgagcagct actaagggat ctagactttg acgcaaactc
     4621 atggaataag gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg
     4681 ctgcagtaca tgtagatact tgcgaggacg ttgattattg gatcaatcga gcatttaccg
     4741 ctgtctacta gggtttctcg tttcggacgc cataaacgag tcgaaatttt ttatgtattt
     4801 ttaagtaaac gaaaaacgat aaaatatgaa caagtcctga ataaagtgaa aaaattgata
     4861 taaaacatag agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa aattatgatc
     4921 cgaatatacg attgggcaca catactatta aggtttcttt ccaacgttgg gattacaaag
     4981 gatttgtgac gtttagaagg ggcggaaatt gtaaaggatt agatgtttta gcattagacg
     5041 aggatgattt atacgatcaa acattgactg ataatccaat tggattcggc ttgctacctg
     5101 aagatgatga aggtaatgaa tggtttaaaa tgacgcttat gaatgataat ggagacgaat
     5161 tatctgttga agatacttgg tcatatttga gtgattatat tgtcagcttt gaaatcattg
     5221 aatttgtagc ggataaagaa gaataaaaac tggaggaata aattttacag ataaaggctt
     5281 agaaggagtc acgacgatta ataaaaaggt agtattacct taggcattga gtaaggaatt
     5341 t
//
LOCUS       DAZAFF010000139         5312 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000139 DAZAFF010000000
VERSION     DAZAFF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.146"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG9BB_002739"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACG9BB_002739"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFN2838596.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACG9BB_002740"
     CDS             complement(1297..2133)
                     /locus_tag="ACG9BB_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2838597.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACG9BB_002741"
     CDS             complement(2317..3534)
                     /locus_tag="ACG9BB_002741"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFN2838598.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACG9BB_002742"
     CDS             complement(3683..3844)
                     /locus_tag="ACG9BB_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838599.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACG9BB_002743"
     tRNA            3917..4000
                     /locus_tag="ACG9BB_002743"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5312)
                     /locus_tag="ACG9BB_002744"
                     /pseudo
     CDS             complement(4240..5312)
                     /locus_tag="ACG9BB_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DAZAFF010000140         5299 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000140 DAZAFF010000000
VERSION     DAZAFF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.147"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..780
                     /locus_tag="ACG9BB_002745"
     CDS             <1..780
                     /locus_tag="ACG9BB_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFN2838600.1"
                     /translation="ERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELAKKNK
                     VKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGTTILY
                     ALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSFESLN
                     VDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTFRKLT
                     DFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            746..1699
                     /locus_tag="ACG9BB_002746"
     CDS             746..1699
                     /locus_tag="ACG9BB_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFN2838601.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2097..3071)
                     /locus_tag="ACG9BB_002747"
     CDS             complement(2097..3071)
                     /locus_tag="ACG9BB_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN2838602.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3186..3620
                     /locus_tag="ACG9BB_002748"
     CDS             3186..3620
                     /locus_tag="ACG9BB_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFN2838603.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(3745..4767)
                     /locus_tag="ACG9BB_002749"
     CDS             complement(3745..4767)
                     /locus_tag="ACG9BB_002749"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFN2838604.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5159..>5299
                     /locus_tag="ACG9BB_002750"
     CDS             5159..>5299
                     /locus_tag="ACG9BB_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HFN2838605.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRA"
BASE COUNT         1666 a         1016 c          947 g         1670 t
ORIGIN      
        1 gaaagacaag aacaaatcat tacattatta gagaaagatt tttatttaac taattcagaa
       61 atcgctaaca aattaggcgt atcagaaatg accatacgcc gagatgtcac tgaattagca
      121 aaaaaaaata aagtaaaaaa gctatatggt ggcgttgaac gaaaggaacc ttataatcga
      181 gaactatcga ctcaagaaaa gattgatacg aatgtagccg aaaaaaaata tattggtcaa
      241 gtcatgaaca gtatgattcc cgataattct actatttatg tcggtgctgg aacaacgatc
      301 ctatatgcac ttcctttcat tcaaaagaaa aatctatttg taattacaaa cagtttgatt
      361 gctttcaact atttaaagaa tcatacaagt tatcggattt tacttactgg tggcgaattt
      421 tctcctatta cagaagagtt cattggggaa gttgcagaaa gatcttttga aagtttgaat
      481 gtcgatatct ctttcgctgc aacaaacggc atatttgata acaatgtgac aaccgctcag
      541 tttattgagg gaaatattca gaacgcagcg ctcaatgcag caaaaatcaa atgcattgtt
      601 gcagattatt caaaattaaa taaatcagac gtctatactt ttagaaaatt aaccgatttt
      661 gattatctaa taaccgataa tcatttatca gaacaggatt tcaatcatta ttcaaaatac
      721 ttaacaatat taaaggagcc caaaaatgat actgacaatc acgatgaacc catccattga
      781 ttccatttat tttacaaaac actttgaatt aggtacgatg acacgctttg attctccggt
      841 acgctatgta ggcggcaagg gaatcaattg cggacgagct tctgctctac taggttcatc
      901 agttatttta acagggtttt tgggcggtta ttttggcagt atgattcaag atatgctgaa
      961 aaatgaaaat ttgtttgatt tgcatttttt acctattgca gaagaatcaa gaggtgccgt
     1021 cacgatcatg catgatgatg aaacgcagac agagctagtg gaagctggtc ctgaaatagc
     1081 agatactact atcaaaaaat taatgaatga tatcaaacta atcagtaaaa ataattacct
     1141 ttcagtcatt acaataaatg gttctgttaa caatccagat ccctttttgt atgctaaatt
     1201 attacaatat atcaaagaac aaatagatcc ttccacctat atattaatgg atatatctgg
     1261 acaacatctc cgtgaagtct tagcaagtaa gtataaaccg atgttcatca aacctaatat
     1321 ccatgagttt tcagatatca tccatcaaaa aattactcaa aaaagcgaca taaaacacca
     1381 tctaaaaaca aatccactat ttaaagatat cgatgttatc atgatttctt gcggctcgta
     1441 tggtgcagta gtcaaagtaa aagaagaatt ttggaatgtt gagatcccta agattcctat
     1501 cattaataca acaggctctg gggatgccac tgttggaggt tttgctcatt cattagaaca
     1561 gaattatcct ttaaaagaat gtgtaaggta cgcaatggct tgcggcatgt cgaatgcaca
     1621 gcaatcagaa gtcgggaaag ttattccagc tgatgtaaaa aatttagcaa aaagcattaa
     1681 aatcacagaa atgaaataaa tagtacaaag taaataattg tttatatgaa atgagacaca
     1741 aagagactgt gacaaaaaac gtgtgtaccg acccccaaaa gttagatttt ttggtctaac
     1801 ttttgggagt cgatacattg tcacagtcct tttttcgaat aaacggtgtt ctcctgccaa
     1861 ctcctcggtc acaagtcaca atattcaaca aacatgagaa gctatttttt gaaattgctc
     1921 cttgtgactc agagttaaac ggcaggctca cgacctcttg gtataaacgg tgttcaaaag
     1981 cttgcatcta cgctaatgag tccaaccaag tcaaaaatga ggagcttttt tggctgtcct
     2041 tattacttga tgcagggcag ttttttcata atctctttta gattaaatcc ttttggttac
     2101 acaaggttct cttcaaagaa tgttgccatt ttttcaaatg gaatgatgtc tgtctggtcg
     2161 tatagatctg tatgggatgc tcctggaatg atcatgagtt ccttgttgtc accttttagt
     2221 tctttgaaag cgtcctcccc aaagtaacgt gaatgagctt tttccccgtg aatgactaag
     2281 actgcatgac ggatttctgg aatataatgc aatagttttg cattcataag tgacaaccct
     2341 gatgtcctat tccaaccatc attggagttt aaagaacgga catgatatcc gcggtctgtt
     2401 ttataatacg catgatagtc tttgacaaat tgaggagcat cttcaggcaa cggatcaacc
     2461 acacctccag cacgtgcgta gtccccttgt ttgaaatcaa tgatgcgttg tgcatttaat
     2521 tcttcacgca tttcatatcg ggcattttca tccataacat cgaagtagcc atttgctgtt
     2581 actcgagtca tgtcatacat tgtggaaaca actgttgctt tgacccgcgt atcgatagct
     2641 gctgcattca atgccatacc tccccatcca caaataccaa tgattccgat tctctcaggg
     2701 tttacatttt tttgtacaga taaaaagtct actgccgctt ggaaatcttc cgtattgata
     2761 tcaggcgaag caacatatcg aggtactcct ccgctttctc ctgtaaacga tggatcaaac
     2821 gcaatcgtta aaaagcctct ttcagccatc gtctgcgcat atagaccaga cgattgttcc
     2881 ttcactgcac caaatggccc gctcacagcg attgccggca atttcttttc acttacttct
     2941 tttggttcat ataaatcagc gactaatgtg ataccaaaac gattcgggaa gctcaccttt
     3001 cgatgattga cttgattgct ttttggaaat actttgtccc attcttgtgt gatgtttaat
     3061 tctttattca tattctagac tctcctttga tcaaaattga catttgtttt tgaatgtatt
     3121 attatgataa aacttaaact tgactttagg tcaaggtaaa aattttattt taggaggatt
     3181 tttttatgta ttccattgga caagtgtctg aaatgtttgg attgcctgtt tctactttac
     3241 gttattacga caaagaggga ttatttcctg atatccagcg tgtttctggt attagaaaat
     3301 tttcagagaa tgagttagag gctttaagag tcattgactg tttgaaaaaa tctggtttag
     3361 aaataaaaga cattaagcaa ttcatggaat ggagcaaaga aggcagtaag acatttgagg
     3421 ttcgtaaaga actatttgaa aaacaaaaag aagtagtaga aaaagaaata gcaaaattag
     3481 aaagagtact cgctatgcta aactacaaaa gctggtacta tgaagaagca ataaaagccg
     3541 gcaatgaaga agccgtattg acaatgattc ctgatgattt accccaacat gtaaaagaag
     3601 cttatgtaca ttctcattga acaaacggat aactcactct ccttctctat gaacttcatg
     3661 aaaacatgaa taaacacctc taactgattg ttagaagtgt ttattttttc actcaagtat
     3721 cctgcactta caaaaatatc ctttttaccg ccatcctaat tctggagcga tatcttttac
     3781 gattgtttca agcaagtgca tgttatattc tacccccaaa gtattaggaa tcgtgacaag
     3841 tactgtgtct gcttctttga ttgcttcatc ttcagctagc tccttgatta attgatcagg
     3901 ttcaccagca aaagtttttc caaaaatcgt tgggtttcgg tgataagcaa gatagccgac
     3961 agcatcttgt tgcggactac cgtcttgtcc aaataagcgt cgatccaaat cagtggtgat
     4021 tggctgaatc gagcgagtaa ctaatgttct tggttcaaaa tcatgacctg cttgcttcca
     4081 cgcttcttta tacgcacgta attggtttgc ttgttggata tggaatggct cgtgggattc
     4141 gtaattgact aatgtagatg attgcagatt cattccatgt tgtgctgccc agattgccgt
     4201 ttgctgcgaa ccagctcccc accagattct ttgtcgtaat ccttctgaat aaggttctat
     4261 ccgtaatttt cctgatcctt gtgggaacat tggttgagga tttggttctt caaatggttt
     4321 tccttcaagt aattccaaga attcaagggt gcgttcccgt gttacctctt tgatttcttc
     4381 ttcactgtaa tcataaccaa agtatttcca gccatctaga acttgttcag gtgaaccacg
     4441 ccccactcct agttgcactc ttccttgcgt gatgatatct gtcaatccgg cattttctgc
     4501 catataataa gggttttcat atcgcatatc aataagtcct gtaccgattt caatcttact
     4561 tgtctttgcg ccgattgccg ctagcaaagg aaacggcgac ccaatctgag gggcaaaatg
     4621 atgtacgcgg aaaaacgctc cgtccactcc gattttttct gcttccaccg ctaaatcaat
     4681 cgattgcaaa taagcatctt gagctgattt cacaagcgat ccttgctcca tccaatgtcc
     4741 aaagcttaaa aagccgattt ttttcaaggt aatgacctcc tttaaaccta caaaaatgga
     4801 aggtttttta tttttaatat gaactgattc tactactttt tttcttaaaa gacgaataat
     4861 tgttctcgcc tcacaatcaa gaaatgtatc ggcttactac acccctactt tcttcatcat
     4921 ctgccgtgtc ttaatctctt ttaacagtct gttacatcgt tgatttttga ctcaaaatga
     4981 ttgtttctaa catgtattta tggtaaaaaa gacaagatta gttctttgta cacatcgatt
     5041 tatacgctga acaaaaagaa atatataaac taagaaaccc attgtttttt cgctccatca
     5101 catagagttt actttagata aacagtgaca tttactaatc cataacggag gaaatcaaat
     5161 gaaaaaaatc tatttcttat gtacgggcaa ttcatgccgc agtcaaatag cagaaggcta
     5221 cgcgcgtaaa tacttgtctc cttcaatgtt tgagataaga agtgcgggga tcgagacaca
     5281 tggcttgaat ccaagagct
//
LOCUS       DAZAFF010000141         5141 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000141 DAZAFF010000000
VERSION     DAZAFF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.148"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..2000
                     /locus_tag="ACG9BB_002751"
     CDS             66..2000
                     /locus_tag="ACG9BB_002751"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFN2838606.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2457..2873
                     /locus_tag="ACG9BB_002752"
     CDS             2457..2873
                     /locus_tag="ACG9BB_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFN2838607.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2887..3453
                     /locus_tag="ACG9BB_002753"
     CDS             2887..3453
                     /locus_tag="ACG9BB_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFN2838608.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3476..4462
                     /locus_tag="ACG9BB_002754"
     CDS             3476..4462
                     /locus_tag="ACG9BB_002754"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFN2838609.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(4617..>5141)
                     /gene="scm"
                     /locus_tag="ACG9BB_002755"
     CDS             complement(4617..>5141)
                     /gene="scm"
                     /locus_tag="ACG9BB_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFN2838610.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         1552 a          949 c         1136 g         1504 t
ORIGIN      
        1 agtatcaaac taaaagaatt atcatatatt cattatatag atagtctata aggagcggat
       61 gatcaatggc aaagcaaaat attgtcgttg taggagcagg atatgccggt gtttcagcta
      121 caaaatactt ggcaaagaaa ctgaaaaaag aagatgtagc aattactctg atcgatcgtc
      181 attcgtacca tactatgatg accgagttgc atgaagtagc tggcggacgt gtagaaccag
      241 aagcaatcca atacgatctg cagcatttgt ttgctcgtca aaagaatgta caacttgtca
      301 cagacactgt aatcggtatc gataaagaac gaaaaatcgt caagaccaaa ttaggttcct
      361 atccttttga tcagctgatc cttggaatgg gcggagagcc gaatgacttt ggtacacctg
      421 gtgtcaaagc acacggattc accctttggt catttgaaga ttctgtaagg attcgggaac
      481 atattgagaa gacagtagca aaagctgcat tggaaccaga tgatgaactg cgtaaagcca
      541 tgctgacttt cgttgtctgt ggttctggat ttaccggtat tgaaatgatt ggggaactga
      601 tcgactggaa aaatcgccta gccaaagatt ataaattaga tccaaacgaa atcacactga
      661 tggtcgtaga ggcaatgccg acgatcttga atatgttgga tcgaaatgat gctgcaaaag
      721 ctgaacgcta tttgcgtaag cacaacgtgg aattattatt gaatgcacca atcgtagaag
      781 tggctgctga tcatatcaaa ttgaaagacg gacgcaacat tccgactcac acactgattt
      841 ggacagctgg tgtcaaagga acttctgacg ctgctgactt tggattagaa tcagcacgcg
      901 gacagcggtt gatcgccaat gaatatatgc aggcaaaagg ctacgaagat aaaaacatct
      961 atatcattgg tgatttagtc ttttatgaag aattcccagg aacaccgaca ccgcagatcg
     1021 ttcaagctgc tgaacaaaca ggacacacag ccgcagcgaa tatcgtggct tcgatcaaag
     1081 gcggagaaaa gcataaattt aaaggaaact atcaaggatt tatggtttct attggtgcca
     1141 aatggggggt agctaaccta tttgataaga tccatttaag cggattttta gctatcgtaa
     1201 tgaaacatat cgtgaatttg aaatacttct ttgatattcg ttctggttat tatatgttcc
     1261 aatatttaat gcatgagttt ttccatatca aagatgatcg caacgtgact cgaggacatt
     1321 cttctcgtta cggcaatgtc ttatggagtg tgccacttcg tattttctat ggtttagttt
     1381 ggttcgttga agcttccaaa aaaatcattg ggaatggtga agtgttcaaa ccaagtactt
     1441 ggtttggtga aggatcatgg ttcactggaa acagtgtgtt cccttggaca tgggaataca
     1501 atgtaacaga tgttgccagt ggtgcttctg ctgctggtga tgcaaccaca gctgccagcg
     1561 gagcaggggc aagtgccgga gaagcagcga ctcaagctgc acattttggt ttgagttatg
     1621 catacggtga agaaccaatg gctgtgattg agaagtcacc agactggttc acaaagatca
     1681 tgcaagtgat catgccaaac aatgatgtag caatgttctt ccaaaaattc atggtattct
     1741 ttgaaattgc cttggcatta gcattgattg ctggattatt cacatggcta tgcagtgcga
     1801 caacgattgt attggtgatc acgttctgct tatcaggcat gttttactgg gtaaacattt
     1861 ggttcatttt tgttgctttc gcattgatga acggttccgg acgtgcgatt gggttggatc
     1921 gctgggtaat tccatggcta caacgtacac taggtaattg gtggtacggt agaccaagtt
     1981 cccagtacgg caagaaataa atagataaag aacgttattt gaatgcaaga tttcaataga
     2041 aaggggctga gacaaaccgt cacagcctcc tttttatgta aaattaaagg aataagatgc
     2101 ccgagataag cctccatgag caagacacag gtgtcacaac ctctgttaca acctcttgtt
     2161 ataaacggtg ttccagaagt agcttcttcg aaaaataagc cgaaatttca caaaaacttg
     2221 aaaagtaatt ttcgtaaatt ccttcttatt tcttgaagtt gcccacttct gtcacaacct
     2281 cttgttataa acggtgttcc acctgctgct tctcggtaat aagcccaaag aattagaaaa
     2341 catggaaagc tgttttctat tcttcgttct tattactcaa agcaagacac aggtgtcaca
     2401 acctcttgtt tttatgctaa aattgggtga gttaatctgg aaaaaggaga acaaaaatga
     2461 gccttaaaga atttgctgaa aaaagtctgc tcaaaccttg ggatgttttt attattttgc
     2521 ttttgactgt tctttctttt ttaccagtca tgatttttgg tcttcagcaa aacaccgcaa
     2581 cagcagaaaa agaagcggta ctgcgtgtgg atggcacaga aatcaaaaca ttccctttga
     2641 tggctggaga agagagcaac acatatactt acaaagatga acacggagat tataacttga
     2701 tcgagataga tggcgatcga gtacggataa aagaagcaga ttgcgatgac cagatctgcg
     2761 tacgtcgagg ctgggcaacg aaaaatggcg aaaccatcgt ctgtttgcca cataaattag
     2821 tgatcgaagt ccgttcaaca gatggaggtg acgaagacag tctgatttat tagaccttca
     2881 cacaccatga gtaaattaca aaaaatgatt tatatttcgc tacttgttgc acaaggagtg
     2941 atcattggtt tattggaaaa tatgatccct tatccttttt cttttgcgcc tggtgcaaaa
     3001 ctaggcttag ccaatctaat cacgatcgtt gccattttta ccatgaaaaa acgagacagc
     3061 ttcttactac tttggctacg actattcctg actactttgc taggcggaac gatttccaca
     3121 ttcctttata gtatgagtgg tgctttgctt agttattttg gaatgttatt ggtcaagcaa
     3181 ttaggaccaa aacgtgtcag tatcatcgga attagtgcgg ccggcggttt catgcataat
     3241 gtcggacagc ttttgatggc ttctttcatt gcacaatcat ggacagtcat gttgtacttg
     3301 ccagtccttt cagttttagg gattttatca gggattgcaa tcgggatcgc agcgaattat
     3361 cttttacgac atgtgcagac acttcaacga ttccaaaaag attatgagca gggaaccaaa
     3421 caaaaatgga gcccaatgtt taccaaaaaa taatcgtcac acaaggagtt tttttatgaa
     3481 gctacatcca atgtgggaaa actatcctgc tttaagtaaa gaattgacag aaacactaaa
     3541 acggatggaa gaaactgtcc gcctgaaaaa caaacctgtc gaacaagcaa tcaaagaaac
     3601 gatccatgcc ggcggaaagc tgttgcgtcc tgcctatcag ctattgttct cacaatttgg
     3661 accagagcaa gatcgagaca aagccatcgc tttggcagct tctatcgaaa tgctccatac
     3721 ggccacactg atccatgatg atatcgttga tgaagcggat ctgcgacgtg gtcagcctaa
     3781 tatccgtagt cagtttggta atgtcgtttc tgtctatgca ggagactatt tatttgtctg
     3841 ctgttttaaa ctattgtctg attactccac ttccttgaaa agtctccaat tgaattctcg
     3901 cagtatggaa aaagtattga atggcgaatt agggcagatg gatgatcgct ataattatga
     3961 actttctgta aaagaatatt tggaaaatat ttcaggtaag acagctgaac tgttccaatt
     4021 aagttgttct gttggcgctt ttgaaagtgg aacaagcgag cgcttcgcaa aaaaagcagg
     4081 agatatcgga ctttctatcg ggatggcctt tcagattatc gatgatattt tagattatac
     4141 aaaagagagc caggaaatcg gcaaacctgt actagaagac atgcgtcaag gggtctattc
     4201 tttaccgttg atctactctt tgcaaaaaaa caaaccaaag ctactacctt atttagagaa
     4261 gaaagcggca atgacagaag aagatgtcga cgccgtacgc aaaatcgttg aacatacagg
     4321 aggcgtggaa gaagctagga agttagcggc ctcttacaca gaaaaggctt tgaaggaaat
     4381 aaagaaatta cctgccacct ctcttcgaac aaaagagaat ctcttttccc ttacccaact
     4441 gattttggat cgcaaagact gaatagggaa aggttcgata cgtaaataaa tttctcagaa
     4501 ggaaatctac gtgttttttg acaaattgaa ttgtagttct tgtatttatg ataaaaagca
     4561 aaaataaaga gcaacgtccg ttagcgacgt tgctcttttt tatcttgaat atttttttac
     4621 cttttcgatt tcttataact cataaaagtg attcttaaac cgattacaag taatataacg
     4681 cctgcgagtg ttatccaatt attcgattga cttccagttt taggaagtgc tctttttgat
     4741 ttgtttccat tgtcattgct tgttttatta gttaagttaa agttcgtcct gttatccgaa
     4801 cttgtttttg agaaggagac acttgcatga cgtgtatcgc ttgtggtaga cgacgtgtct
     4861 gtactatcgc ttgaagttga tgaatctgag ctatcacttg tggtagagga tgtatctgta
     4921 ctgtcacttg aagctgacgt gtccgtgcta tcacttgtag tggtagatga atctgagcta
     4981 tcacttgtgg tagaggtagt atctgtactg tcacttgaag ctgacgtgtc cgtgctatca
     5041 cttgttgtgg tagatgtatc cgtactgtcg cttgaagctg acgtgtccgt gctatcactt
     5101 gtggtagagg tagtatctgt actgtcactt gaagctgatg t
//
LOCUS       DAZAFF010000142         5091 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000142 DAZAFF010000000
VERSION     DAZAFF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.149"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..160)
                     /locus_tag="ACG9BB_002756"
     CDS             complement(<1..160)
                     /locus_tag="ACG9BB_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFN2838611.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHW"
     gene            318..581
                     /locus_tag="ACG9BB_002757"
     CDS             318..581
                     /locus_tag="ACG9BB_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838612.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            605..793
                     /locus_tag="ACG9BB_002758"
     CDS             605..793
                     /locus_tag="ACG9BB_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838613.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(876..1061)
                     /locus_tag="ACG9BB_002759"
     CDS             complement(876..1061)
                     /locus_tag="ACG9BB_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838614.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            1276..1647
                     /locus_tag="ACG9BB_002760"
     CDS             1276..1647
                     /locus_tag="ACG9BB_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFN2838615.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(1797..1869)
                     /locus_tag="ACG9BB_002761"
     tRNA            complement(1797..1869)
                     /locus_tag="ACG9BB_002761"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1834..1836),aa:Gln,seq:ctg)
     gene            complement(1915..2343)
                     /locus_tag="ACG9BB_002762"
     CDS             complement(1915..2343)
                     /locus_tag="ACG9BB_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838616.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(2403..3161)
                     /locus_tag="ACG9BB_002763"
     CDS             complement(2403..3161)
                     /locus_tag="ACG9BB_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFN2838617.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            3456..3653
                     /locus_tag="ACG9BB_002764"
     CDS             3456..3653
                     /locus_tag="ACG9BB_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838618.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3759..4241
                     /locus_tag="ACG9BB_002765"
     CDS             3759..4241
                     /locus_tag="ACG9BB_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838619.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(4299..4895)
                     /locus_tag="ACG9BB_002766"
     CDS             complement(4299..4895)
                     /locus_tag="ACG9BB_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN2838620.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         1652 a          792 c          978 g         1669 t
ORIGIN      
        1 cccaatgcct aaaagaaaaa atttgataag taaaatatac agcaggtaaa gaggccagta
       61 aaactgaaaa cttctttttt gtccatttta tgtaatagaa aagaatgctt atcataataa
      121 aaaaaggaat ataaaaaaca aattgaccga tcgcgatcat actattgcct ccgataaata
      181 aagtccatga tacaaaataa cactttgtgc gaaatggcgc aagcagtttt gtggatttat
      241 ccggtaaaat ctgtatcgat tttgaaatta gaggagaaac ttgctataat caaagtgaaa
      301 gaaggagtgg gattttcatg gatcatgtag tgttagaaat cgatttgcat ttgatcaata
      361 atattcgggt gatttactat gttgtaagta acagtgtcga acaacgtgta ctgacgaata
      421 aaataaatgg tattttagca aagaaagacg ttcaccgatt caataatggt gaaggaagtt
      481 attatagtat cccagtagaa aaaattattt atacgacagt caaagtcaga gaagatctag
      541 ataccaaaaa agcatatgaa cctattttta caacttattg attgacagca gatggaggaa
      601 aaaaatgaat ccatatgaac aacttattca agatttgatc gatggaaaaa tcgaaaagat
      661 tgatgtaaaa agagaagaat taatggcttt tcgagaagcg tggctgaagt tggagaatcg
      721 taaatatgtg gtgggaaaag cctcactgaa cggcaatgtg acttatcatt acgatccgac
      781 acgtttattt tagtatgaag tattcagaaa agaaccaaag gaggacgatg cagcagcatt
      841 gtcctccttt gattttacaa aagtgaaagt tttacttatt tgaatagatt tttaaatttg
      901 tttttgacac tgtcgatatt tacatctttt aaaagatttt gggctttctc tttataaggt
      961 cctaattgtt ctttatgttc ttcgataaat cgttttgcgc catcgaaatt gccgttctta
     1021 atcatttcct tgactttttg aaataattct tcttgattca ttttgaacac atcctttgct
     1081 atcaacatga ttttttacat ggacaaaacg agaaaaactg atttatccac acgtcaataa
     1141 tatcaaataa cgatcatttt tactaaaaaa acactcgatt ctcgcttcta aagtaggaga
     1201 aaaatctcca acttgggacg gatggaattt ctttaactga cccatatact gaatatataa
     1261 caaggaggta gaaagatgaa agtgattaaa tatttgattg gactggcggc gattagtggt
     1321 attttgctat ttggactgaa atattatact gaaggaagtt atggagaaat ccctggtata
     1381 cttgatcagc taaatccatt agttgaaaaa ggagaggtct atgtaaagac acaaaaacca
     1441 gaggaagtaa atgaatatgg tacagcaagg tatgtgcaga aagctgctga tgccaatggg
     1501 aaagaacgaa cggttgaatt taatggtctt tctgtgttaa aagaaaatca ttatttgaaa
     1561 attagcaata aaggagcaca catagaaacg tatgaggaag ttcctagaga aaaagttccg
     1621 gagaaagcat tagcaattat tgggtaaatc agtttttgta ttaagaatac gcccgatttt
     1681 attattcata atttaattat gtaaactaaa tacaagtagt aaaaataata caataatccc
     1741 cctgcaaaaa gattgttctt ttacaggggg ttcatttttt cagcaagcac atcaaattgg
     1801 gatggcaggg ttcgaaccta caattgcttg attcagagtc aagtgcctta ccagtttggc
     1861 tacatcccat ttattcaaca aaataatcat agcactttcc taaaaaaacg caagttattt
     1921 ttcttgatcg gatagttgtt gctttactcg taaaaggcgg cgtactagtt tcttttctaa
     1981 tccagcagga agggcaatca gttctgctac tgacaagacg gtgtccaata actgatattc
     2041 tttgacaggt tcattcataa tatgatagat gtttgctaaa cgttcaatat attcaaagcg
     2101 agtatactct gttcgagcaa ttagaaattt caataaatcg gtggctttct cgtactcttg
     2161 atctgcgtaa taaacaagag attgacgata caatttgatt tccagttcat tgagggtagc
     2221 aggtctgcga cgctcgttcc actcattaat agagacttta ccttgaacat ttcccttgca
     2281 gtcacggaca aaaataatcg gaatttcatt tggatccatt tttttatgtg tgaatttttc
     2341 cattatctac gctcccttca aaggtttggt atcttgagta taagggattt ttgtttgaat
     2401 tactagcttt tctccaaagt tagcatctgt ccacccattg cagtgaaaat cgccgaaaaa
     2461 tcgttgatcg gtactgctcg attttttaca ccatagggat cgttgacata aatattttct
     2521 ttgtcatatc cagtgactac acaagcatga acaagaggag tgactcttac ttctccatta
     2581 tttgtggtcc aagtcataaa atcagtttca tctgggactt caaaattgat agtcacagaa
     2641 gtccagacag gatttccttt agatagtgct tttttgatat catcgagttg agcatcggtt
     2701 tttggaaaag tgtgataatc cgtttggacg atttgctgag caagggaggc aactggggga
     2761 acatagacga ccatagccca gtctccgcca ctgatatcac caacaaatcc ttcattagga
     2821 tcgccatgag tcccatctgc attgaaaaca gggacgtaat ccaattgctc agctaactgg
     2881 tttttagttg tttcataacc ataaaaagat aaaagcatag aaagcgaagt gacttcacag
     2941 ccattttcta aagaaggaga atcaaactgg ctctctaatg gaacatctag taaaacttct
     3001 gctttctctt ttgtcttttt cgttttttgt gttgaacttt tttgtgcagt gttcattgta
     3061 tgttctggag aatggatatg taggtaataa cttgtaccgc ttaataaaag agcagctgtg
     3121 ccccctagaa acaatagttt tttgaacgtt tttttcttca tttgaaatct cccttttcag
     3181 ctgtttatct ttcctattat aaatgtattt ttattggaaa tggaagtatt atattttaat
     3241 atgtaggaaa taattagttt ttaaggaata tgttttaatt ttttgaaaaa aattcttcta
     3301 aatacaacag ctgttttaat tgtgaaaacg aaaacagtac gctataatgc ctgtaaggtt
     3361 gatttttaac tgtaaataaa aatcaatgag ttaaatctcc aaagaaatag cagtggtaat
     3421 gtggagaaaa gttccagcag tttaggagga ttttgatgac ggaagaaatg attaatttag
     3481 gagaacaata tgcatgcaaa ccaattggtt ttacaaaaac ggtcattggt gaagttgtat
     3541 caaaaatgac aaactgcgcc gtggtcaaag ttgcccaatg tgctgcagag gaccaagaat
     3601 tattagatga aaaagcaagc atggttgttg caaaatatga tacatttgaa taaagaaaac
     3661 aattggacgt gcggcgtgct gcaagtccaa tttctttgag aaggtaaatc agtattcttt
     3721 tcctattttc tggagaagaa aaagcgaggc gagttgtcat ggtaacattc tttacggttc
     3781 tattgttttt attcgttgtt gtgatgtttt tatttctttg gctggttcga aaagaaatta
     3841 tttatcggac tgtgcgaaat cgatgggtct acttggtgat acccttctta gtgtcactag
     3901 tcatttggta tacactcatc agtcatccta cagcagatga attggcaaaa gggatcttgt
     3961 cagcaatgat ttttattagt tttttgttgg acagtagagg aatcacagaa gaaggacttg
     4021 ttttgaatag ctttgacaaa aaaggtgttc ctttttctga gatcaataaa attgtattat
     4081 atcagccaaa aggttctaaa atcgtaaaaa tgaatttttt ccgaaacgga tggcgaggac
     4141 cgatgctgaa attttctgca tcattagaag aattagttcc atttttgtcc caacgcttaa
     4201 acgatgaggc agagattgat attatgattg atccagaata aaaacagagg ttgcgacaag
     4261 atgtttgtca caacctctgt tttttatttc aaataaaatt atccaaatac gcggaagcga
     4321 tcggcatcgt tcatgtattc tttttcctca gccggtgttt ctggtgaacc gaatactaat
     4381 tgcgaacgta gtttccagtt tgaagggata ttccattctt ttgcaactgc ttcgtcaata
     4441 actgggttgt aatgttgcaa gtttgcgccc aatcctttat caactaatgc tacccaagta
     4501 ttagctgttg cgattccgtt tgattgttct gaccaatctg ggaagttgtc tgcatataat
     4561 tcaaattgtt cttgtagacc ttttaccacg tcagtatctt tgaagaataa tactgtacca
     4621 taaccatttt tgaaaccagc taatttgttt tgtgtatttg ggaaagcttc tgctggagtc
     4681 aatgggcgaa gtgcttcttc agtgatttcc caaagttttt catgtgcatc tccgaaaagg
     4741 ataactgcgc gtgttgattg agcattgaaa gcagttgggc tttctttaat tgcttctttg
     4801 atcaaagttg ttaattcttc attagaaagt gaaacattac gtcctagatg atagattgaa
     4861 cggcggtttt tcaaagtgtc agtaaaagtt gtcataataa aatttctcct ttgatttgcg
     4921 ttttgcattt ttttaattga ttacgaaatg aagtatagca ccttacaaaa agtaagtaaa
     4981 cagatatgct cacaaaaaat aagcgaagtt caaaaaacaa ttttaataag aaaaatcgtt
     5041 atgtaaggat tttcttagtt atcgctttca ttgttgtgtt ttaggaattt t
//
LOCUS       DAZAFF010000143         4970 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000143 DAZAFF010000000
VERSION     DAZAFF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.151"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..459)
                     /locus_tag="ACG9BB_002767"
     CDS             complement(145..459)
                     /locus_tag="ACG9BB_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838621.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            745..984
                     /locus_tag="ACG9BB_002768"
     CDS             745..984
                     /locus_tag="ACG9BB_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838622.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1041..1169
                     /locus_tag="ACG9BB_002769"
     CDS             1041..1169
                     /locus_tag="ACG9BB_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838623.1"
                     /translation="MQPLQMLYDNKPELNDPDISFAFFEMIGSDDLFKTTSLSIRK"
     gene            complement(1346..1606)
                     /locus_tag="ACG9BB_002770"
     CDS             complement(1346..1606)
                     /locus_tag="ACG9BB_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838624.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1791..2288)
                     /locus_tag="ACG9BB_002771"
     CDS             complement(1791..2288)
                     /locus_tag="ACG9BB_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFN2838625.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(2418..3128)
                     /gene="budA"
                     /locus_tag="ACG9BB_002772"
     CDS             complement(2418..3128)
                     /gene="budA"
                     /locus_tag="ACG9BB_002772"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFN2838626.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(3141..4805)
                     /gene="alsS"
                     /locus_tag="ACG9BB_002773"
     CDS             complement(3141..4805)
                     /gene="alsS"
                     /locus_tag="ACG9BB_002773"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFN2838627.1"
                     /translation="MAEKMKQGSKSVVERLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
BASE COUNT         1507 a         1011 c          759 g         1693 t
ORIGIN      
        1 tataaactct agagaaatta attcatccta acataaaata attgttcact caaaagtttt
       61 tctagaacaa ttaaaacagc aggcttcaca ttcgctatac atttgtaaga agtaataatt
      121 cctgtactaa aaggcataat tgctttaatt attttttgac ttctcttcaa acacaagaag
      181 tagttgtgta ataattgcag aaataaggat tccaataata gatataaaat ttgacatgga
      241 aacgtctata acattaaaag aataaagtgt tgaagaaagt aaactgattg aaataacccc
      301 tccgtaaaaa atagtctgtg aatataccca ctttttttca gaagacgtac ttcgtttggt
      361 tctaaaacct atccctgcat ttctactctt atcaaaaaac caaatattta gaagtaataa
      421 tattgctatt attaagataa aattactgta aaaatccaaa agctatcctc ctagcaacta
      481 tttaaatacc caaaaaaatt acctctagat aatatcatac ctacgttttc acttataaat
      541 tttttcataa aaaaagcgtt tagaggcata atcgttaata cagatggtaa ccaatgtatc
      601 tggtttgaac tgggatatca ccctctccat atatgtatta ctaagactaa tagatactat
      661 aaaagacttg atagttttgc attgattttg cctaatactg agcataggtg tatgctaaac
      721 gatactataa ggagtgtgta cataatgaaa agaattctgt ttatcagttt atcaaattta
      781 ttgttactta ctgcttgtgg gacttctgca tcaaagaaca taccttcaga tacgcaaatt
      841 tcgaatagta tcgattcaac aatttctaca aatgagaaaa attctccggt agaaacttcc
      901 gatataaaag tttctacctc tgcaaaaaac agggtaaaga aactgtcagc acgtctcctt
      961 tatttcaaac aaactctgaa ttagcttctt catcttcttc agcaaataat gatgatagtt
     1021 tcgcgtttac tcctaactaa atgcaacctt tacaaatgct ttacgataac aagcctgagc
     1081 taaatgatcc tgatatcagt tttgcatttt ttgaaatgat tggatcggat gatcttttta
     1141 aaactactag tttgtctatc cgaaaatagg acagttctgg cagtataggt ttttatcgag
     1201 tttctccacc aggcctcgtt acagagacag attctctagg cgaacctttt gaataaatca
     1261 aaaatcatga acaatcactg ccaactaaat aaagtgcctt caaaagcttg gtcttcacgc
     1321 ttttgaaggc actttattta aatctttatg aaactaaagt atgttcatat ttgttgctat
     1381 agttatatag atattctgtt gtttccaact cataaacgtg agtatatttt gccactactt
     1441 ttccgcattt ttctaatatt tcattgcgat cgacgtcatg atattcgtct acgcagatca
     1501 caacacaatt ttctaattta ctgattactt ctccaatcac tttttgtttc aagccaatag
     1561 gttggcaaat atattttttt cctaaaatga tagtttcgtt attcatattt catccatcct
     1621 taatgttttt tcattattta aaatgataat aacattaaaa tatgattatg aaaatcaata
     1681 acacttgtga ttttataaaa aaataaaaaa accgaaaaac agaaatgccc actgtctttc
     1741 cggctctttt tattcttgat ttctcttctt ttttgtttca atagacgatt ttagttatta
     1801 ataaatgaaa aagcatttat attaaaattt gctaataaaa gatctacata tttttctaga
     1861 aatgcttgat cgatttcatc ataccctttc gttactccgc aatcgatatc taaaacaccg
     1921 attaaattac cttcttttac cattggcact acgatttctg attgtgcggc tgaatcgcag
     1981 gaaatgtaat ttgcatgggt tttgacatca tcaacgatca atgtttcttg tttttcagct
     2041 gattctccac aaactccttt tcccatcttg atacgtgtgc aagaaactcg cccttggaac
     2101 ggtcctaaaa tcaattcatt accatcgaat aaataatatc ctgcaaagac agtctcaggc
     2161 aaagtctcat tcaacaatgc agatgaattt gctaaatttg ctaaccagtt atcttctgct
     2221 tctaataatc cttgttgctg taataataaa atttcgtatg catctttttt ctgtttttct
     2281 gtccacatga taaaaaccgc tcctttttga tctatggaga agattatagc tcagtttttt
     2341 tgtaaacgct aggttttctc ctcaaaaaaa cgagatacgt caggttaatc accttgaacg
     2401 catctcgctg tctttttcta ttctgcttgt tggatttctg ctgctaaatc agcataatca
     2461 atcgtttcgt gaacaaaagt cggatcatca actggaaaat gctgtacaag ggacgagatg
     2521 cggctaatct caacttttcc ttcagccact tcaaaatcca ttacatgtcc gccaaatgta
     2581 tgtgtatcat cgataaaatg caggtgaaat cccgctgcag caactccttg gaataattca
     2641 ggcgtaaaga acccgactaa tgttccttgg acattgcttt gttggaattc tggttgagac
     2701 tctgaaattt ttgctagtct tctgtaaggt ttttcttgct tcggcatgat acggatgtgc
     2761 atatttttga ataatccagt tatcttcact gccaaaaata ggttttcccc ggcttctctt
     2821 actaatatgt cttttttcaa ttgttctcca tctgcagttt ttgagacaga aaaactagta
     2881 tctggcgtaa aatctgtaat tgctgcatag ggggtaagtt cacttccatc taattgggca
     2941 actgagccat ctgatcttcc ttgataagct acgccatcta aaaagatgac ttcaccatca
     3001 agcccatgca atgttcccaa tcccagagtt cctttttcta atatatgagc aatcgtttct
     3061 gttccatcca tcaagccggc catcaaagct cctaatgtcc cgtgctggta taagattttt
     3121 tcagtcatgt cgacacttcc ttaatagaat tgatctggta agattgtttc tcccaatttc
     3181 tcattatcac ggtaatcaat cggaatatca ataattacgg gtccttctgt cgcaaatcct
     3241 tcttctaaag ctttttctaa ttcctctaca cttgtagctc ggattccttt agcgccaaaa
     3301 gcttctgcat atttcacaaa atcaactgga ccaaagtcta cacctgacgc ccgctgatat
     3361 ttcatttttt cttggaactc taccatattg tagtgtccat cattccaaat caaatgaacg
     3421 atattcaatt ttttacgtac cgccgtttct aaatcctggg cagagaataa aaatccccca
     3481 tcgccggaaa ctgatacgat ctgtgtattt ggacgcacca atgcagcaga aatcgcccat
     3541 ggcaatgcaa caccaagcgt ttgcatacca ttactgaata acaaatgacg aggttcataa
     3601 cttctaaagt ggcgtgccat ccaaatataa tgactaccta catctacggt aacagtcata
     3661 tcatctgtta cttttgactg aagcgtgtta ataacttcta aaggatgcaa gatacctgct
     3721 tctccttttg attgaacaat gtcccgctcc gttaattttg cttgaagaga tgctaaatat
     3781 tcttttgcat cttctgacac ttgttgaggc tctaatgaac ctgtcaataa atcaagagtg
     3841 gctgagatat caccgatcaa ttctctttca ggctgcataa aaggatcgat ttcagctggc
     3901 gcttcatcga tcacaatgat ccgcgcatct ttttccgcat tccagtttct tgcttcatat
     3961 tcgattggat catagccaat tgcaataact agatcacttc gtttcaaaag catatctcct
     4021 ggttggtttc taaataaacc cacacgacca aagaaatgcg cttctaattc acgtgagatc
     4081 actccagctg cttggaatgt ctctacaaca ggcaatgctg tcttttcaac taattgcctg
     4141 atggctaacg tttctttttc acttgatcct cgcataccta ctaatagtac cggtagtttt
     4201 gcttctctaa tgcgttcagc taggtaacgg atatcatctg cagaagcaga acctaattta
     4261 ggatcgctca atggcttgat gacattacct tggacaggtg catccactac atcttgcggg
     4321 atactgataa agctcgcgcc tttttttgaa ctcttagcca tacgataagc attggcaatc
     4381 acttctgaaa ttgtttctgg atcttggatt tctgcactgt attttgtgat tggctggaac
     4441 agtgctgcat tatccatgct ttgatgggtc aacttcaata gatcgcttcg ttttacttgt
     4501 ccggcaattg ctaaaactgg gtcaccttct gctgtcgctg ttaccaaacc tgtagctaag
     4561 ttactagcac caggaccact tgttgcgata actactcctg gctctcctgt aatacgtccg
     4621 attgcttgtg ccataaatgc agcattttgt tcgtgacgag tcacgattaa ttcaggacct
     4681 tgatcctcta attcattaaa tacaccatcg atttttgcac ctggtatacc gaatacataa
     4741 tcaacatgat gattaatcaa cctttctact acagatttcg aaccttgttt cattttttca
     4801 gccattgaac atcgtccttt ttatgcttta tttttaatta ttagttagaa ataatcacaa
     4861 ataagtgata cagtttctaa acctgtttca ttcaaaagta tagcatgttt tatttcattt
     4921 gtttagccat atgtttcaaa aaatgaaaaa gaatgacagt ttttacataa
//
LOCUS       DAZAFF010000144         4961 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000144 DAZAFF010000000
VERSION     DAZAFF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.152"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1338)
                     /locus_tag="ACG9BB_002774"
     CDS             complement(139..1338)
                     /locus_tag="ACG9BB_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFN2838628.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..2844)
                     /locus_tag="ACG9BB_002775"
     CDS             complement(1468..2844)
                     /locus_tag="ACG9BB_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2838629.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2844..3500)
                     /locus_tag="ACG9BB_002776"
     CDS             complement(2844..3500)
                     /locus_tag="ACG9BB_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFN2838630.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3510..4787)
                     /locus_tag="ACG9BB_002777"
     CDS             complement(3510..4787)
                     /locus_tag="ACG9BB_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFN2838631.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4806..>4961)
                     /locus_tag="ACG9BB_002778"
     CDS             complement(4806..>4961)
                     /locus_tag="ACG9BB_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFN2838632.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1556 a          903 c          608 g         1894 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacgtacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat
     1921 taatgagcca aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa
     1981 tgcagggtaa tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt
     2041 cccaagatct tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc
     2101 ttggtataca gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt
     2161 gattaatgta ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc
     2221 gaatagacct ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc
     2281 aaataaatta gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa
     2341 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
     2401 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
     2461 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
     2521 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
     2581 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
     2641 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
     2701 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa
     2761 agttgacata gataaaataa ctaatatgat aattaatgat tttaaacttt ttctagttac
     2821 ataagcaaat gcatttttta tcattattca acctccaaat tcattttatt tacttttttc
     2881 aactttttac cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt
     2941 gttacaacta ttacacattt atttctatct ttagctaatg tctttaatat attaattatt
     3001 tctccagcag taacactgtc tagattacca gtaggctcat cagctagtat tattggagca
     3061 tctgatacca atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata
     3121 acatttcttt ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct
     3181 gatttattta ctaatctaat attttcaatt ggtgataaat aatctattaa attatagttt
     3241 tgaaatacta aagatatatt attttttcta tgattactat atcctttatt ttgtatatct
     3301 tcattcttaa acaatatttt tcctgtttga ggtttatcaa gtccagcaag taaggaaaga
     3361 agtgtagatt ttcctgttcc tgactttcct actatcgcat aaaacttccc aagttcaaat
     3421 ttttgattta ctcctgacaa aactttttct ttagaatttg cataactata tgctacattc
     3481 tttatttcta atatatccat tattttctcc taacttattt ttgataagat ttctttaggc
     3541 ttcttgatta acattaatga agaagcaaat acaactgata aaacaataat acttattaat
     3601 attaaataac tttgtccaag tgttgttata tttgacataa aactactatt atttattaaa
     3661 cttccaccgg aaatcattga atcctctgag ttaatgaatc catctacaat tacttttagt
     3721 aatacatttc ctaaaaataa ggaagatatt atacttggta tcgatatgaa tattaactcg
     3781 aatataaatt gcattataat ttgtatctta gatgttccaa tagataagaa tatacctatt
     3841 tcataaattc tttcccttaa ccatagaatc aagattaatg aaagaacaac catcccacct
     3901 aacataatag aataagtcat tacttttatt atatatttta ttccactcac tgactctaaa
     3961 gactcttcaa acgcattaga atctttttca acaaaatact ttgactcatc aattttaagc
     4021 tctttcaatt tgtttaaggc taagtctgta gattctgcac taccagaata cattaaaatt
     4081 ttatttgcaa tttcattatt ttctgaatta tttaatatct cttggcttgt tgaataatct
     4141 acaaacacca tattttcact aaaatcagaa gataatcctg tatatgtttc ctgtttttta
     4201 ccagaaaaga tccctataat tttaaattta tgacttttta tttttccact tttttcaata
     4261 tctagtaatt caagatcaac ttcatcacct aattttagat tgttttgttt agcaaattct
     4321 tcatgaacaa taattgaatt cttatcattt tcttctatat ttttaccttc tttaattgta
     4381 aatactccac tactaaataa aatatttctt ttagtattat ttgtagcttc gagtgaaaca
     4441 acattcttaa attcgtcaga taaatcttct ctatttattc tttgttctcc gctaactact
     4501 ttagcatctt ttagttttgc taatccatca tattgaatta ttttttcttc aatttctttt
     4561 aatttttcaa tatctttaaa ttgattaaca ttaaaatatt taccatcttt ttttgttatt
     4621 gatattgaag aattagaact ttcatataaa gccttttcta tttcattgct tgatttcatt
     4681 attgttaaac aagaatacaa gcaagaaaga actatagtta atatgataaa aattattaaa
     4741 gttctatttt tctttcttgt aatatatgct atagcatttt ttatcacttt cctaactact
     4801 cctctctatt ttcttactcc ataactaatt gcatccactg gacaagcatt tttacatcgt
     4861 ccacatctga tacactccag atgattgcaa ttctcaactg aatctttatt catttggcaa
     4921 acttttttac attttccaca attaatacac ttatccttat c
//
LOCUS       DAZAFF010000145         4832 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000145 DAZAFF010000000
VERSION     DAZAFF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.153"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..972)
                     /locus_tag="ACG9BB_002779"
     CDS             complement(220..972)
                     /locus_tag="ACG9BB_002779"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFN2838633.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="ACG9BB_002780"
     CDS             1085..2005
                     /locus_tag="ACG9BB_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFN2838634.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="ACG9BB_002781"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="ACG9BB_002781"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFN2838635.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="ACG9BB_002782"
     CDS             3311..4789
                     /locus_tag="ACG9BB_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2838636.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVSICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT         1698 a          778 c          932 g         1424 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagaaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcagtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg at
//
LOCUS       DAZAFF010000146         4720 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000146 DAZAFF010000000
VERSION     DAZAFF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.154"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..1174
                     /locus_tag="ACG9BB_002783"
     CDS             164..1174
                     /locus_tag="ACG9BB_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HFN2838637.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAYID
                     SNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSN
                     ELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERD
                     FTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVET
                     IEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYY
                     LS"
     gene            1542..2966
                     /locus_tag="ACG9BB_002784"
     CDS             1542..2966
                     /locus_tag="ACG9BB_002784"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFN2838638.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNPFLTIIFSAI
                     FVFTYKQLIITYLKKFFRIKMAKR"
     gene            3052..3480
                     /locus_tag="ACG9BB_002785"
     CDS             3052..3480
                     /locus_tag="ACG9BB_002785"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFN2838639.1"
                     /translation="MIFGKKHSLVEVPEFGSEPYLIEIGDNFRCSFGVKFITHDGGVW
                     VLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANTLVNKDIPNNS
                     VVAGSSCKVISTIEEHYEKNKNEFIKYRTSMILKQKNFFY"
     gene            3568..>4720
                     /locus_tag="ACG9BB_002786"
     CDS             3568..>4720
                     /locus_tag="ACG9BB_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFN2838640.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDI"
BASE COUNT         1609 a          623 c          874 g         1614 t
ORIGIN      
        1 tatatttttt gagtgaatgg agaagacgca tagtatcata aacttcgtta tatcttaaat
       61 tatggggtat gcctaaataa taaatgtaat acgagtataa agaagaaagc aattttgggt
      121 ttaaccctat tggttccttt cagaaaaagg tggatgaata aaaatgaaaa tattaataac
      181 aggtggagca ggatttattg gttttcattt gtctagaaaa ttactcaagg aaactgaaaa
      241 tcaaattgta gcgattgata atctcaatga ttattatgat gtccaattaa aagaagatcg
      301 attaacgata ctgtacgatt ataataattt taaatttatc agaatggatc tattagagaa
      361 agaaaaatta gattctctat ttgaaagtga aggttttgac atagtaattc atttagctgc
      421 tcaagcaggt gttagatatt ctattgaaaa cccttatgct tatattgatt ctaatattat
      481 cggttttctt aatattcttg aagcctgccg aaacttccct gtaaagcatc tattatatgc
      541 atcatctagt tcagtatatg gtgggaacac gaaagtaccg ttttcaacag aagattcggt
      601 ggatcatcca gttagccttt atgcggctac caaaaaatcg aatgaattaa tggctcatac
      661 ttatagccac ctttataaaa taccaacaac tgggctgaga tttttcactg tctatggtcc
      721 atttgggcga ccagatatgg cctatttctc ttttaccaag aatattttat ctggaaaaga
      781 aattaagatt tttaaccatg gaaagatgga aagagatttc acttacatcg atgatatcgt
      841 agagggaatc actagattaa tcaataaagt acctgaaccc aactccgact ggacagacat
      901 cgatgatttg agtagtgaaa gttgggcacc atataagata tacaatattg gaaataataa
      961 gccggttgca ttgttggagt ttgttgaaac aattgaaaag tatttaggtg ttaaagcgaa
     1021 caagattttt ttggacatgc agccgggtga tgtcgttaga acttatgccg atattgaaga
     1081 tttagaggaa aaaatcaact ttaaaccgaa tataaccttg gatgagggca ttaaaaaatt
     1141 cgttgaatgg tatacagtgt attatttgag ttaattttga aaggaatatt gaagatgggt
     1201 aattcatacg atttttttag ctttgttagg tattttagga aattctactt tcaaacgtga
     1261 cgaactttct cgccgaatag atattttcta taactaaaag aatttctata aaacaaaatt
     1321 gtgtatcaag atgtgtgaac tggattctga ctgccaatat tgattgatga gattgaagaa
     1381 atatatagtt gatcatggat atctttattt gaaaagatct tagctattca catattcaaa
     1441 aaaattttta ctacgaggac tcacacgata cagaggaatt taggtagttc taattaaacc
     1501 agaaagtcat ctatagatgt gagttgaggg aggtcatacg gttggatagt agcagtaata
     1561 aatcacattc aatagctaaa aaagctttta tttacgcaat aggaacatta tcatcaaaaa
     1621 tgatatctgt cattataatc cctatctatg cagtatatat ttctgttgcc gatttaggaa
     1681 gctttgatta tcaaaataca attgctcaat ttctttctcc agttattttt ggagcaatat
     1741 gggaagcaat tttacgcatg ggattaggca aagaaaaaaa tattcagaaa aaagtagtga
     1801 cgaacagtat ttgttttgtt atagcaaata cagtgatatt agccattttt ttaccaataa
     1861 tatatagaat gattttcccg aatttagagt attatcaatt gtttgttttt caattaattt
     1921 tcactcctat cctaagtgtt tttcaatata ttgtaagagc actaggtgaa tcaaagattt
     1981 ttgtgatgag tggtataatc gctgcgggta tcaacctgtt actccttact atttttgttg
     2041 ttacaatggg tatggggata cttggtctct tactctctat cataatttct aatgttataa
     2101 atttgatttt tttgattttc agggggcaga tctttcaata tttcgcaacg agttcttttg
     2161 attttttatt tttaaagtcc atgctgaaat actctatccc actcactttg aatctcactt
     2221 ttggatggct aatagttggt ttttcaagat tttatattgg aacgtacatc ggagtaacag
     2281 aaaatggtat tttttctttt gcgatgaaat tttcatctat aattttaact cttacaagta
     2341 ttgttagtat gtctatgata gaggatgcgg ttatcacgtc tgagaatgat gatttcatcg
     2401 aaagattcgt tgagaaaaat acctatattt ttaaaatgta cattaggtta gcgactcttt
     2461 ctattcctat catttgctgt gcatactctt ttataaccaa ccctgattat aaaaaatcat
     2521 tagttctagt tccaatatta gttattatgg catgtttcag tgggatgaca acaaatatcg
     2581 gaaatatatt tgcagtatac aataaaacaa atcagttatt aatgatttca gtagtgtcag
     2641 gtctattgag tgttgttggc acctatttat taggctattc ttttggagta attggtgtct
     2701 catttgctac tttgctcggt actttagtat tatttggact tagatattat tttggaagga
     2761 aaattgctta ttatgcattt gattggaagc gaatatcgct acttttagtt ctctattccg
     2821 caatcgcttt tgtatgcatt catagaggct tctattttaa tcctttctta acgattattt
     2881 ttagtgctat tttcgttttt acgtataaac aattaattat aacgtatcta aaaaaatttt
     2941 tcagaataaa aatggctaaa aggtgagggt atacgaagta agaatagaat ctttcgaaga
     3001 ataaagagaa aactatttgg aaaaaatctg cgagaatgta ttaatgaagg tttgatattc
     3061 ggaaaaaaac atagtttagt agaggttcct gaatttggga gcgagccata tttaattgaa
     3121 ataggggata atttcagatg ttcatttggt gttaaattta taacacacga tggtggagtc
     3181 tgggtcttaa ggaagatgag ttctgtgagc gagtcaactt caaaaatagg taggatcaaa
     3241 ttgggaaata atatttttat tggtatttac tctattgttt tcccaggagt ttcaattggc
     3301 gataatgtaa ttattagagc gaacacttta gtaaataaag atatcccaaa taatagtgtt
     3361 gtagcgggta gttcatgtaa agtgatttca actatagagg agcattatga gaaaaataaa
     3421 aatgaattta ttaaatatcg aacctcgatg attttgaagc aaaaaaattt tttctattaa
     3481 ataatattga ttgaataagg aatatgttca aaaggataac agctaaaata ctataaaatg
     3541 aaatttgatt ggagcgtatg gaagaatatg aaaatttcag ttttcggatt aggctatgtt
     3601 ggtctagcaa attcactatt attagcacaa aaagagcagg tcaaagctta tgatatcgtg
     3661 gaagaaaaaa tcacgatgtt acaaaatggc atctcaccaa tagaagataa agaagttcat
     3721 gaatttttaa aacgggatga cttaaatgtc gaatttacgt caaattttac tgatgcagta
     3781 aacttcggtg attacttgat tattgcaact ccaacagatt atgacgaaaa gaaaaactat
     3841 tttaatacat caacagtaga agatgtaatt gagaaggctt tagcaattcg tccagattca
     3901 acattcatta ttaagtcgac agttccagtt ggctatgcaa atgaaattaa agcgaaattt
     3961 ggtacagaaa acatcatttt ttcacctgaa tttttgcgtg aaggaaaagc tttgtacgac
     4021 aacttatatc catcaagaat tatagttgga gaatgttctg ctcgtggtcg cgagattgca
     4081 actatgtttc aaagaaacgc atttaacaaa gatgcatcag ttcttttgac tcactcaact
     4141 gaagcagaag ctattaaatt attctcgaat acatatttgg cattgcgagt ggcgtatttc
     4201 aatgaattag atacgtatgc agaatttcgt gggcttgata gtaagcaaat tattgaaggt
     4261 gtcggtttgg atccacggat cggttcgcat tataataatc cttcttttgg ctatggtgga
     4321 tactgtttac ccaaagacac aaaacaatta agagccaact atgaaaaagt accaagcaat
     4381 atcatcagtg caattgttga tgcaaataca acaaggaaag attttattgc tggacagatt
     4441 ttggcgaaaa aaccaaatgt tgttggaatc tatcgtttaa cgatgaaggc cggttcagat
     4501 aattttcgat attcgtcgat ccaaggaatt atgaaacgga ttaaagccaa aggaattgaa
     4561 gtggttgttt acgagcctac attggatgaa gatgaatttt atcattcaca agtattgaaa
     4621 gatattaatg aatttaaaaa tatttctgat gtgatcgtat ctaatcggtt tgaagatatt
     4681 ttaaaggatg ttgaagaaaa agtttatacc agagatatct
//
LOCUS       DAZAFF010000147         4590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000147 DAZAFF010000000
VERSION     DAZAFF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.155"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACG9BB_002787"
     CDS             186..2855
                     /locus_tag="ACG9BB_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFN2838641.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3434)
                     /locus_tag="ACG9BB_002788"
     CDS             complement(2946..3434)
                     /locus_tag="ACG9BB_002788"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2838642.1"
                     /translation="MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIY
                     DRNDLAMVLIGMPGIEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDI
                     KLEDFSDYEAITTIIKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVI
                     GI"
     gene            3680..4219
                     /locus_tag="ACG9BB_002789"
     CDS             3680..4219
                     /locus_tag="ACG9BB_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838643.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="ACG9BB_002790"
     CDS             4216..4554
                     /locus_tag="ACG9BB_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFN2838644.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1421 a          790 c          859 g         1520 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggagaaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgacatt
//
LOCUS       DAZAFF010000148         4581 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000148 DAZAFF010000000
VERSION     DAZAFF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.156"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..512)
                     /locus_tag="ACG9BB_002791"
     CDS             complement(42..512)
                     /locus_tag="ACG9BB_002791"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFN2838645.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(671..1129)
                     /locus_tag="ACG9BB_002792"
     CDS             complement(671..1129)
                     /locus_tag="ACG9BB_002792"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFN2838646.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1300..2445)
                     /locus_tag="ACG9BB_002793"
     CDS             complement(1300..2445)
                     /locus_tag="ACG9BB_002793"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFN2838647.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2458..3375)
                     /locus_tag="ACG9BB_002794"
     CDS             complement(2458..3375)
                     /locus_tag="ACG9BB_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFN2838648.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3803..4366
                     /gene="efp"
                     /locus_tag="ACG9BB_002795"
     CDS             3803..4366
                     /gene="efp"
                     /locus_tag="ACG9BB_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFN2838649.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1427 a          837 c          869 g         1448 t
ORIGIN      
        1 gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac ggattcaatt
       61 gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac tttgccattt
      121 ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt accaggagct
      181 tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc aatcaattga
      241 tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc agtcgctgaa
      301 tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc ttgtgtctga
      361 taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca ttttgttgca
      421 gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac agtgccgtct
      481 tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta ttttatataa
      541 taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac tcttttcaga
      601 caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca tctttacgtt
      661 cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa aattcacgag
      721 ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt gtttcattgc
      781 ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga acatctaaaa
      841 tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg catcccattg
      901 gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc aagtgctcaa
      961 gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag cggacgtcgt
     1021 atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt acataaggtg
     1081 ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt tgtaagactc
     1141 ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag tgaaatttac
     1201 aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc aatctgaaat
     1261 attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga ttctgtcaaa
     1321 tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag aaagacggat
     1381 caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg catgagtcat
     1441 gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta aagtaaatag
     1501 ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa aagaaatcat
     1561 tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat cgggtaatcc
     1621 tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta ccacaaaagc
     1681 atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct gaagtaacca
     1741 gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga tctgatcggc
     1801 tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc cgcctagata
     1861 tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt gaaaataagg
     1921 agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca gagtggcact
     1981 tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat aaagtgcttt
     2041 tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta tgatcgtgta
     2101 ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc catagacatc
     2161 atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat gaatcccaga
     2221 aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa tcagttcttc
     2281 caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag gctggccgac
     2341 gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc ctgttgtggg
     2401 atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc tttcctccta
     2461 taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg taacgacacg
     2521 atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg caccactcga
     2581 acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa acccttctac
     2641 atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg gaatgaattc
     2701 tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca cagagccctc
     2761 tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg tagaagttcc
     2821 agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt ctttgatttc
     2881 atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt tattttcaaa
     2941 ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt tgctgatggc
     3001 accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg ctttcatcaa
     3061 ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta atttgtatgt
     3121 gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga tgatcgtcgt
     3181 tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc ccaatctatc
     3241 tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag aacctttagg
     3301 catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag cttctgtaat
     3361 tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag ctgactatta
     3421 aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa gttacctggt
     3481 actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt ttatgcacca
     3541 tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc cctccaagta
     3601 aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa agaaaaaaat
     3661 atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg agaccttaaa
     3721 agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt gattatgata
     3781 aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg gtatgacttt
     3841 cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac ctggtaaagg
     3901 aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat acgacacaac
     3961 gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg tacaatattt
     4021 gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc aatatgaaat
     4081 tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg aagtaaaaat
     4141 ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg tattacgtgt
     4201 tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta agccagcgac
     4261 aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg aattgttgga
     4321 aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg ctaacggatt
     4381 gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga tctgcctttc
     4441 aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat tttatttata
     4501 ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt tatgaagaaa
     4561 ttgaaatgat tcataaaatt g
//
LOCUS       DAZAFF010000149         4512 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000149 DAZAFF010000000
VERSION     DAZAFF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.157"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..1111
                     /gene="gap"
                     /locus_tag="ACG9BB_002796"
     CDS             101..1111
                     /gene="gap"
                     /locus_tag="ACG9BB_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFN2838650.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(1196..2131)
                     /locus_tag="ACG9BB_002797"
     CDS             complement(1196..2131)
                     /locus_tag="ACG9BB_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFN2838651.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVAPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            2317..3276
                     /locus_tag="ACG9BB_002798"
     CDS             2317..3276
                     /locus_tag="ACG9BB_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFN2838652.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(3362..4456)
                     /locus_tag="ACG9BB_002799"
     CDS             complement(3362..4456)
                     /locus_tag="ACG9BB_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFN2838653.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         1403 a          921 c          782 g         1406 t
ORIGIN      
        1 tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt aattgaggat
       61 gccgtttcac agcaccccta ataaaggagg aacttttgat atgacagtaa aagtaggtat
      121 taacggattt gggcgtattg gccgtctggc tttccgccgc atcaaagaag tctcagatga
      181 tattgaagta gtagcaatca acgacttaac aagtccaact atgttagcac aattattgca
      241 atttgactct actcatggta cttatcctgg cacagttact gcaacagaag actcaatcgt
      301 agtggatggc gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc
      361 aaaagaaaac ggtgtagata tcgtactaga atgtactggt ttttatacat ctgaagaaaa
      421 agcaaaagcg catttagatg ctggtgtcaa acgagtagtt atttctgccc cagctggtgc
      481 tatgaaaacc atcgtctata acgtcaatga tgatacatta gatgaaaacg acaaaatcat
      541 ctcagcaggt tcatgtacaa caaactgttt agcacctatg gcttatttct taaataatga
      601 atttgggatc gaagttggta caatgacaac agttcatgcc tacacttcca cacaaatgct
      661 cttagatgga cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg ctgacaatac
      721 tatcccacat tctactggtg ctgcgaaagc aatcggctta gttatccctg agctacaagg
      781 aaaattacaa ggacacgcac aacgtgttcc agtcgttgat ggttcattaa ctgaattggt
      841 ttcagtattg aaaacaaaag taactgctga tcaagtaaac gaagcaatca aaaaacacac
      901 agttgacaat ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg
      961 aacaactcaa ggttcgatct ttgacccaac gcaaacagaa gtaacaactg ctggtgattt
     1021 ccaacttgtt aaaactgttg catggtatga caatgagtat ggatttactt gccaaatgat
     1081 tcgcttacta gaaaaattcg ctaacttgta atttgataga tcaatcgata aaagaagcct
     1141 ttgactggat ccagtcaaag gcttctttct tttcatcccc tacttttaaa ctcgttcaat
     1201 gaacatattg gcttaaaaat tgttggacta cttgctgata tttttcagga tctgtcttgt
     1261 atgcttccgc atgctcggct ccttttacaa catacttttc ttttggtccc ttcgttgcac
     1321 gatagacctt atctaacatt tcaaatggta caaatgtatc ttcgtctcca tgtataaaga
     1381 gcattgggcg ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc
     1441 cagcacggac ttttgtcact agactcgtta cttgcataag cggaaatgct ggaagactaa
     1501 acatatcgtc tagctgataa gcaagctcat catgaacgga agaataaccg caatcttcaa
     1561 tgattgcttt cacattatct ggtagttttt ctccgctagt catcataact gttgctgctc
     1621 ccatgctgac accgtataga acgatttctt gcgattttcc attgttttct agcactttgt
     1681 ttatccactg tacatagtct ttacgctctg gccatccaaa tccaatgtaa tccccttcgc
     1741 tttttccatg tcctcttgca tcaggaacaa ggacattgta acccatatca tgaaacatct
     1801 ttgcgtaatt ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca agagctgttt
     1861 tttcactttt cgtttcagca ggaagataaa tcgctgataa tttcagaccg tcttttgaag
     1921 tgatggtcca atcgcttcgg ttttcttttg aactaaacca tttatgtgca tccgtttctt
     1981 tggatgtctg tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca gaaggcgcaa
     2041 cagcataatt ataaaaataa ttccctgcaa acactaacgc aacaatcaga gctaaagcga
     2101 cgcctaaaag gattttcatc caaagtttca taaatcatcc atctcatttc tcttttttca
     2161 tataaccttg aattagtata atcaaagaat ctctactatg caatgttcat ttttataacc
     2221 cgcgaattgt aaaattaagt tagaaaaata aaaaggcatt tatggtacac tcaaattgta
     2281 tttccgacga aagaaaacaa aggagtgtaa ccataaatga cctacaaaca tcttaccata
     2341 gacgaactga caatgataga atcatattat cttcaacata ataagccggt tgaaatcgct
     2401 aaccgaatgg gacgtgctat acaaactatt tataatgtag tcaataagtt caagcaaggc
     2461 aagactgctc ttgattattg gcaccagtat aaagaaaata agaaaaaatg tggtagaaaa
     2521 gtcattcaat tacctgctca tgaagtagat tacattaaag agaaagtcac tcttggttgg
     2581 acgcctgacg tcattatcgg gcgaaaagaa aggtctgttt catgcggtat gagaacactt
     2641 tatcgtttat tttctaaagg aatatttgat attgacacac taccgatgaa aggtaaaaga
     2701 aaacccaatg gccatcagga aaaacgggga aaacaacagt atcagcgctc aatccatgat
     2761 cgacctgata attatcctga tttcaattct gagtttggtc accttgaagg tgatacgatc
     2821 gttggcattc atcataaaag tgccgtcatt actttagttg aaagattatc taaagtcatt
     2881 atcacgatta aacccaacgg ccgtaaggca ttagatattg aaactgccct taatcaatgg
     2941 ttttctcgct tccctaaaaa cttctttaaa tctattacgt ttgactgtgg aaaagaattt
     3001 tctaactgga aagccattag taaccaacat gatattgata tatattttgc ggaccctgga
     3061 acaccttctc aacgcccatt aaacgagaat tctaacggga ttctgcgtcg taatggactg
     3121 ccgaaatcaa tggattttag aaaagtgaat cagacattta tttccagtgt cagcaatcaa
     3181 cgtaatcata ttccaagaaa atcattgaat tacagaacac caatcgaaat atttttgagc
     3241 tatgtacaag aagcatttta ttctagctta atttgacaaa tcataaccta taaaaaaaca
     3301 aaataaaaaa gcagaaataa ctggctattt ctagcggatt atttctgcaa taactaatta
     3361 tctatttttt attttcaatt aatttaaagc ttgaccatgg tttcaaaaag tcaaggagga
     3421 ataattcgtc atcggagatt cttcctacaa cattgacacg tccgtcgttt ttcatatcct
     3481 tcaaggcgat ttgtgtttct cctttgtatt gaccgtaccc ttcattgtca atcagtacat
     3541 ccccatgatg catatctcgc gtattatgag gcgggaaatc tttatctttg tagtatacac
     3601 gagtcatcgt tgaacgaagg atatattctg agcgatcccc tcgataacta tgaaggttat
     3661 caaacaagca gattcgttcg ttttctgtaa taccttcttc cgtatctact ttcaatgttg
     3721 gataatccgc gttaaatgct tctgccattt ctttcaattc ttcttcactt gcgtacgcat
     3781 ttccaataga aatatcatca atcaatcctg taaggattaa atgcttcact tgtgtcgcaa
     3841 tttcaagatc tcggtggtct tccagcgtgc acaagccatc ttgcgtaggc caagggccaa
     3901 actcagcaga ttgcgaattg acgaatgcca tcgtattcaa attatatttt ctgaattttt
     3961 ctgaacaaaa aacaaagtgt tcgtatccta aaccagagta tcgatgagga taaaaattat
     4021 gagaacctaa taaattatcc gtgtttggcg aataactcat gatgttgtca acgtaattcg
     4081 tgcccgaact catattgatc tcgattttga taccaaaagg attgcgcgtc atttttgctt
     4141 cttctgctcc tgtgaatccg atatctaagc ggaccccgta ggcacccatc ttatggaaaa
     4201 aagataagtc atcgtaagaa atctccaact gttcaaataa cgctggattg atatcaacca
     4261 ttacttccat gcctaggcta ttcgcgtaat caactacttc tttaaattct gaaagaactt
     4321 tttctttatc atcattgatc tgcagcaaac tggtaaaaac ccgtttatac ccatacttat
     4381 gagctaaatc caagtacgct ttgtcctttt caaatgttga ccgttctgga taaatcgaga
     4441 ttcctaattt tcccatatta ccatttcctc ctgcatcttt tcgtttttgt atccacttac
     4501 attatagcaa ac
//
LOCUS       DAZAFF010000150         4502 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000150 DAZAFF010000000
VERSION     DAZAFF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.158"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(255..740)
                     /locus_tag="ACG9BB_002800"
     CDS             complement(255..740)
                     /locus_tag="ACG9BB_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFN2838654.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(742..1413)
                     /locus_tag="ACG9BB_002801"
     CDS             complement(742..1413)
                     /locus_tag="ACG9BB_002801"
                     /inference="COORDINATES: protein motif:HMM:NF037286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838655.1"
                     /translation="MSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTP
                     FTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLS
                     LRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENI
                     VVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLIN
                     SIQED"
     gene            complement(1676..2869)
                     /locus_tag="ACG9BB_002802"
     CDS             complement(1676..2869)
                     /locus_tag="ACG9BB_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2838656.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(2953..3153)
                     /locus_tag="ACG9BB_002803"
     CDS             complement(2953..3153)
                     /locus_tag="ACG9BB_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFN2838657.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3577..3810)
                     /locus_tag="ACG9BB_002804"
     CDS             complement(3577..3810)
                     /locus_tag="ACG9BB_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2838658.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(3817..4248)
                     /locus_tag="ACG9BB_002805"
     CDS             complement(3817..4248)
                     /locus_tag="ACG9BB_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFN2838659.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1138 a         1090 c          864 g         1410 t
ORIGIN      
        1 ttcatcttga ttggtattat aatcgtattc aagttgcgtg gcgtgctttt gctagaaaat
       61 atataactgt tctttataaa gaaatataag gattaaacat tttttatcat agagttttac
      121 ttatctatag aattatgtct gttcctaatt cagtttgatg aattaactga taaaaaaata
      181 tctactattt aaagatacag ggataagtaa gtaaaaaaat aacatgtcat tagatagtag
      241 acagttccaa ctctttattt accttttttg tatgctgcac tgtcaatcca agttcctaaa
      301 ttgttgtatc catcatcact tatataatca tattggccat caataatttc gtcaaaccac
      361 aaatattctc catctggttt ttgcccaatt attttaatta ctgattgact agtagttgtc
      421 atctggttaa aatcttttat tttacctata ttaacggcca aaatagcatt tcctcgctta
      481 taactctcct ggatttcata tttaacatat tctcgatcaa gagtttcttt acctatcagc
      541 accacagtca ctgatgttcc aattaattga ttatctatcc agttttttat agcaatatct
      601 ccattacgtt taactttctc aaattcagcc ttatctacaa aaccagcttc ctttgttcct
      661 cgcaaagtcc agctgtttct aactaccatt gctcttgtta catctgcata atggaaacta
      721 aaaaaaaccc ttctcaccat attaatcctc ctgaatacta tttattaaac tgacaatttg
      781 ttcaactttt ttatctactt ctccttcagt atctaatttt tctagcgctt gaaatttact
      841 ttttaaggaa tcatcaaaat aatcactgaa attctcttga atcttatcaa acaaaacctt
      901 acttgcacct ttggtaaatc caatcggaac tactatattt tcattttgaa cagataattc
      961 aaattcttca atcattccat ctgctgtaac tatttcacct gattcaagtt tgtttccaaa
     1021 aacaaaaata ctaatcccag actctgcaat aatgttttct cgatatcttc tccactcatg
     1081 aggcatatca aggggaagtg gtcttaaaga aagtacgtct tgtatgttat tttttcttaa
     1141 atttcttctc tgctttagaa ctcctgaaac aatactatta ccaattccaa gtccgtaacc
     1201 tgatactatt ctataaccgg attcagaaag cctatatgct aatttctcga caaattttaa
     1261 tcctttcgat tcagatgtaa atggagtata atcaacagca cttcccgaaa taaaaatagt
     1321 ttttctcaaa tacttttttc taatactttt caaaatatca gtaatctcag aataatcatc
     1381 tacttcaaca acgtttatcg aaaatctgga caaatcattg atttgtagtc ttctttttgt
     1441 cagttcataa ttatatctct cttgatcaaa aaaatcctca gaatcgacaa aattttcctt
     1501 taaaattata tgggatttta gattatcttt ttagaagttc ttgaatgtcg gtatagcccg
     1561 tgtttccggg cttttccggc atttttgata ttgttagata cttacaaatg ttcttattac
     1621 ctctcaccat ttccccctca aaagtagtaa atggagtagt aaaaccggcc ttgtgtcagg
     1681 ctgccagccg ttccatttct gcccttgcgg aatcgaatgt ggcgtgtgca taatagttca
     1741 gcgtcatggt gatattggaa tgtcccataa tgtactgcaa ggctttgggg ttcattcccg
     1801 cattggctaa ccgggtgcag aatgtatgac gcatagcatg gggcgtcgtc gttttcggca
     1861 acgcttcttc atggctcttg ttgtacttct tcacaagcct gccaaacatc ccgccataat
     1921 ctaccgccgt catgggatag ccgttctgtt tgagaaacag gaaattggca tacccgtcaa
     1981 taatgaacgg ctttgcacct ttgcggtttt ttatcactcg ctggaacgcc tgatacactt
     2041 cctcattcat ggggattttt ctgacgccgc tttgggtctt gggttcgtca atatagtaac
     2101 cgacgccggt atttctcaaa agctggtggc tcacgatgat tacccggttt tcaaaatcca
     2161 aatctttgtc cgtcagtccg cacagttccg aaatgcgaag ccctgtcccc agcagaataa
     2221 cgattgcgtc ataatgcttt tggtaaacct tatcaccttg aataaaggac agaaaacttt
     2281 cttcctgtgc tggtgtaaga ggcacctttg gttccgtatc gtcgtccaga acgtcactaa
     2341 gctggaagtc aaacgggttt tttcggatgc aatcgtcctg tatggcggtg taaaaagccg
     2401 ctttcaggga acgcttgtcg ttattgatgg tcttataaga aaggcccttt tctttcatcc
     2461 gaagcgccca ttcctttgcg tcggacagct tcacactgtc aatcgggcag ctccccagcc
     2521 gatcttgttc cagaatacgc atcagccgtt cccggcccag tttggtgttg tgcttcacat
     2581 tgccccggtg cctgatctgc ttggcgtaga gctggcagac ggtcattttc ttgccgatgg
     2641 tgtctatccc gtcgtcaagg tctttctgga tttctttctc cttttcccgg agtgaaatat
     2701 cctcccgttt acctgccggg gtcttatccg taggcactaa cttccatgag taaacaaact
     2761 gcggttttcc aaaggtatca acatatttgt aagcatatcg tccgtctttt ctctggctct
     2821 ctccagaccg caaaacgcga tttttgctgt ctcgtctttt ttctgacatc tcgtcatgct
     2881 cctttccatg acggaaagag ccgcatatat gcttataaat tattataaca tatctgcggc
     2941 cctttttcac tattagattg tgtccaaagt gtcaattatt ttctcaaact gcttgcgttt
     3001 gatctggatg cggttgccat tcataatgac ccagccagag gccggatttt cctcggccag
     3061 cttacgcaat ttgttttctc cgatacggaa atatttagac gcttcctcaa tcgtcagcgt
     3121 gtatttttcc caaataggca catcatggtt cataatgcag ccccctttca caaattcaaa
     3181 ggtttgtgtg atttaggggc atagacggac ggctgctggc acagctccac gggactttca
     3241 cccccgcgtg gttctcacgc ggccctaccc attgcctgcg acgcttttaa cgctcggact
     3301 gtggctataa gggagtatca tagtcctgcc tgtacgtcgt cgcccgcaag ccgccacgct
     3361 cacattgcgg cgtggtgttc ctcgctccgc ttttcccgta gggaaaagta tcatggcgca
     3421 ccagccgtcc ggctcggcac agacagaatg tgtccgtatg ccctatgatg cgccgccgtt
     3481 atcttgcggg catatttatc aaggtgcgat caggccaccg ggaaaacacc gaaacgggga
     3541 aagggtaagc cgtttcatgc tgccggggcc ggttcgtcag ttcgtgacga tagcgtggat
     3601 gagcttgatt tccagacggc gcttcatggc ctcgtccaga cacacatgcg gatagccgtt
     3661 ttcgtcatag agcgtccggg tacacagctt gttcatgtaa ccgctgtaat actgcaaaac
     3721 gcgctctacg gctccggcat ccccagcgcg ggcggcctcg atcaccgaaa ggggcaaaag
     3781 ctcaatgccc ttgtagttcg ggttcttcat ccgcgtctaa cctcccttcg gcatcaacgc
     3841 ctttagctgt ttccgcagct ccgataaggt atttgtcctg tgccgctgaa cggcactccg
     3901 ggacattccc acaaggccgc cgatctcacc atctgccata tccagaacac agtgcaggat
     3961 taaaatactc tgctccatgc tgggcagcgc ggcaaaggct tcggcaacaa gctcattgtt
     4021 gatatgtagg tcatagccat gcgacgaaaa cgtaaagctg tcgctggggt agtgatctac
     4081 tgtacagagc ttgtccaaat ctgcctgtga caaagagctt agtgacactt cacggtcacg
     4141 gcggcgcttc atttcagcca agtagctttt ggcctcgtta cgcagtaccg tcttgcaaaa
     4201 agcatcgaac atgcaccgcg cttcatagtc gcgggggata gcatccatct tctcaccccc
     4261 tttctgtggg ggacttaggt ggttctctcc ctttccgcta acaagacttt gacaaggccg
     4321 ccagctaccc gctaaatacc tcctttttga aaatttctca aaaaattttt ttggggacag
     4381 ccgggccgca aaagacgaca aaaatcgccc ggcaggtcgc aaacctaccg ggcgagaaaa
     4441 tgctttgcta tgctgctgat ttacatggta tagtacatac tcataaccgg aatagccctg
     4501 tg
//
LOCUS       DAZAFF010000151         4427 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000151 DAZAFF010000000
VERSION     DAZAFF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.159"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..1386
                     /locus_tag="ACG9BB_002806"
     CDS             52..1386
                     /locus_tag="ACG9BB_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFN2838660.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            1427..2338
                     /locus_tag="ACG9BB_002807"
     CDS             1427..2338
                     /locus_tag="ACG9BB_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2838661.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            2335..3189
                     /locus_tag="ACG9BB_002808"
     CDS             2335..3189
                     /locus_tag="ACG9BB_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFN2838662.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            3186..3803
                     /locus_tag="ACG9BB_002809"
     CDS             3186..3803
                     /locus_tag="ACG9BB_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFN2838663.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            3805..4323
                     /locus_tag="ACG9BB_002810"
     CDS             3805..4323
                     /locus_tag="ACG9BB_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFN2838664.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1324 a          758 c          898 g         1447 t
ORIGIN      
        1 tatcgttata ttaaaaccgg catcggttac attaaaattt ggaggtaaaa tatgaaaaaa
       61 ttattgttta cagggttgct ggcaagttca gcattgtttt tattaggagg atgcggatca
      121 agtaatgcaa gcgatgccag ttcaagtgca gcaaattctt ctgaaaaaac aagtgatgga
      181 agaaaaaata ttgatttctg gtccttttgg ggatccggtg cacgaaaaga agctatcgaa
      241 gaaatcattg aggatttcaa tcaatcacaa aacgagatcc atgtaaatta taagtatcag
      301 ccatggggtg acatctggac taaatcttta tctgcaataa cagctggaaa tgcacccgat
      361 gttattgtac aggacataaa ttccgttgcc caaagagcag aagcacagca agcaactaat
      421 ctctccaaat atgtagaaaa agacacttct aaggattttt atccgcagct ttgggataca
      481 gtagaatatg aaggtgatcc ttatgcgatc ccattcaata cagatacaca agtcattttt
      541 tataataaaa agttatttaa agaagctgga atcagtgaac aagatctacc gacaacttgg
      601 gatgaattag aagaaacagc acgtaaacta gataaaaaag aaggaaaaga atttacacgc
      661 atcggttttt atccattatg gaatttaggg gctgatgtat gggcgctaaa cgcagataac
      721 gggactagtt ggtttgacaa agatgaaaaa gtcaagattg atactaaaaa taaagtggaa
      781 gccttggagt ggatccaaga ctggcaagat tattatggga aaaatacgat caataaatta
      841 gaagccgaat ttgggtctgg tgtatctgat ccatttattt ccggattagt agccatgcgt
      901 gcccaaaata tcaattatta ctctagtttg atggaaaatg caccggatga ttttgatttt
      961 ggcgtgatgc agctcccaga aaaagaagaa ggttcaggcc attggtcgtg gggtggcgga
     1021 ttcgtcttgg aagtaccata cggagcaaaa gatcctgagg cctcttatga attcatcaaa
     1081 tatctttctt ctccagaagt acaagaaaaa ttcggcgaaa aaagttttga tatcatggcg
     1141 agcaagacag ccaatgagaa tttagtgaat aatgacaacc taacggataa aggaaaaatg
     1201 atctatcaaa tggcggacga aaactttaaa aatacggtga tcacaccagt tcctttagat
     1261 gctccggatt acacgacatt agtgaatgaa cagattgacc agattctttt aggaacaaaa
     1321 tcaccaaaag aaggattggc agatgcacaa aaagcagtag aaaacttagt caagcaaaat
     1381 caataatcca tttttgactg tctaggtaca gaggaggcaa gaaaatttga agcgtttatc
     1441 tatccgaaag aaaaaagaag caagatgggc ctatttattt atctcgcctt ttattatcgg
     1501 gttcttggta tttatgctag ggcctatgct tttttcagtt attggtagtt tcaccgatta
     1561 taatttgaca tccaagatga atttcattgg tctagcaaat ttcaaaagaa tgttcgttca
     1621 cgatgattta ttttggaaat cgttatacaa caccgtttat ttcgtcatct tcaatgtacc
     1681 gctgacgacg attttcagtg tgtttttagc cacagtactc aatcaaaaaa tcaaaggaat
     1741 cagtgtattc cgtacagctt tctatttacc ggcagtctta tcaggagtag cagtctacat
     1801 cttatggatg cagctgcttt ctccatctgc aggattgatc aacacatttc tgggctggtt
     1861 ccatatccct ggaccagctt ggctgtttga tccacaatgg acgaaacctg cgttgatttt
     1921 gatgaaaata tggagttctg gaggagcaat gctactttac ttagctacat tacaaaatgt
     1981 tcctagatct ttatatgaat cagcagaaat cgatggagca ggcgtttggg gaaagttcaa
     2041 aagtattact ttgccattaa tcactcctat tattttttac gatgtgatca cctctacgat
     2101 cggttctttc caaatttttc aagaagctta tgtcatgacg aaaaatggaa caggcggacc
     2161 tgcaaattct ttgctttttt ataatctgca tatgtggaac aaggcgttcg tagcttttga
     2221 tatgggttat gcgatggcta tgtcaatgat cctgtttctg atcgtgttgg ttctgacgtt
     2281 tgtcaatttg aagcttgcac ctaagtgggt ctattattct ggaggtgacg gcaaatgatg
     2341 gattatttca aaaagaccag tgtgaatcgg caaacaaaaa aaatactaag ttatgtcctc
     2401 atgacagtaa taggaatcat tttgatcatt ccattgcttt ggatggtgtt tacttcattg
     2461 aaaccaatgg aagaaatcgt tcgttatcct ccgacctttt tcccagaaaa aatcgtatgg
     2521 gaaaactatc tggatacgat tgccgccttt cctttttggc gctatgcaag aaatacatta
     2581 ttcatcactg tgctagtagt gatcggtaat gtcctttcaa attcttttat tgcttatgga
     2641 tttgcgaagc ttgattttcc tgggaaaaag ctgatgtttg ctttggttct ttctacgatg
     2701 atgatcccgg ggtttgtaac gatgatccct caatatgtat tattctccaa gatcggctgg
     2761 gtaggtactt atttgcccct gatcgttcca tcgttcttcg gcaatgcgtt caatatattt
     2821 ctgatgcgtc aattttactt atcgatcaat aatgaattga tcgaagctgc tgaaatcgac
     2881 ggagcgaatc atttgtatat ttggagtcat ttgatgcttc cattgacgaa acctgccttg
     2941 attaccattg cgatcaattc cttcaacgca gcttggaatg atttcttagg tcctctgtta
     3001 tatatccaag atcaagaaaa atacacgtta cagattggct tgcaagtttt tcagaaccaa
     3061 gcaacgacac aatggaatta tctgatggca ggagcaacac ttgtcttgat cccgacgatt
     3121 ttgctgttct tttttgcaca acgttatttc attgaaggga tggatttgac aggaggttca
     3181 aaaggatgat gaatcgatcg attggtaaaa aagccaatga ttatttctta gaaatcattg
     3241 aatggattcc acgtcttatc catttacagc tactatggtt cctgtgcgtc ttgcctgttt
     3301 tcacattagg aactgcaagt cgaacagttc tgtatatgat ttatcattac aaaaaaaatg
     3361 aagagaaaaa gctcggttca ctattctgga aaaagtttcg tgagaacttc tctctttatg
     3421 gaaagcaaga tagtttagca agtgtttatt ttcttcttct attgatcgat tatcagattt
     3481 tccgctattg gggtggggga attggctata cattgatgta tactacgttg tttttgcttc
     3541 tattaagcgc gcttctcttt agttacaaaa tcttgcttca actggaacaa aaagaagctt
     3601 cttggattac tgcatttttc ttgtttttta atgatttcaa aagcgcgctt ttctatttgg
     3661 caggcacgtt gatccttctt gtcgcattct ggtttgcagg accaatctat tttgctttat
     3721 taggtttcag tttcttgttt tattttcaag tcctgctttt tatccatcgt acgcaagaaa
     3781 aggttcttaa aaaggaggaa taacatgttg cgtttgattg ctaaaatcga tgatagtatt
     3841 atcaacaagt tcgccaagca acggaaaacg gatagtcgaa aaatcaattt tttgaaattt
     3901 atcagtaata tctctaatgg tggttttatt tggttgctcc ttgctgctgt tttagtcttg
     3961 atacctgata ctagagaata tggtctagcc attcttatcg gcagtttgat ctattttttt
     4021 gttggtttgc tttttatcaa aaaaacgaca agacggaaaa gaccttatga aaaaaatcgt
     4081 cggcttgtcc tttacacaag taaaccgaag acttcttctt ttccttcagg acatacagcg
     4141 ctagcctttt tatcagtagc tgcgttgttc gaattaagtc cagttgcagg aatattagcc
     4201 tttttatttg cttgtttgat cgcttattcg aggatttttt tacttttaca ttatcctact
     4261 gacattttag gaggagctat tttaggaagt gttattggca tcctatgcgc tgccgttatc
     4321 tgaattaatt gtacgaataa agcatgagtg cgtaaatgcg catttgtgct ttattttttt
     4381 gtttcgagga aagcgtagtt gtttattcat ttgtcttaat aacttaa
//
LOCUS       DAZAFF010000152         4398 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000152 DAZAFF010000000
VERSION     DAZAFF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.160"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACG9BB_002811"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACG9BB_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFN2838665.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACG9BB_002812"
     CDS             complement(2020..2922)
                     /locus_tag="ACG9BB_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN2838666.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACG9BB_002813"
     CDS             complement(2919..3611)
                     /locus_tag="ACG9BB_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFN2838667.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACG9BB_002814"
     CDS             complement(3654..4295)
                     /locus_tag="ACG9BB_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFN2838668.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1347 a          945 c          761 g         1345 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctccctt
//
LOCUS       DAZAFF010000153         4365 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000153 DAZAFF010000000
VERSION     DAZAFF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.161"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..1075
                     /locus_tag="ACG9BB_002815"
     CDS             152..1075
                     /locus_tag="ACG9BB_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFN2838669.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1081..2334
                     /locus_tag="ACG9BB_002816"
     CDS             1081..2334
                     /locus_tag="ACG9BB_002816"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFN2838670.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2468..3814
                     /gene="gor"
                     /locus_tag="ACG9BB_002817"
     CDS             2468..3814
                     /gene="gor"
                     /locus_tag="ACG9BB_002817"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFN2838671.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1504 a          690 c          939 g         1232 t
ORIGIN      
        1 tcacaatctc tttttatatt tggacagaat atatcgaact ttattttgat tttttgtgaa
       61 ataattttca aaaatccgac tttaaataga agaagtattg gaaagttttt agagctatgc
      121 tactattatg aagttgtagg aggagaaaat tatgatcgaa ctcataacca cagtagaaac
      181 tattgaacaa gcagaacagt tgcttgctgc aggtgcagat acactatatt tcggtgaaga
      241 gcaatttgga ttgcgtctgc ctgcttcatt tacacgagaa gaacagcgac aattggtaga
      301 attagcacac aagtatggta aaaaagcgaa tattgctgta aatgggatca ttcatcctga
      361 aaaaatgaaa aatgtacctg aatatttggc ttttttaaaa gaaataaacg tagatgtgat
      421 cacagttggt gatacaggta ttatttatac gatgaaaaaa aatcctgaat tgcgtatacc
      481 gtatatttat gatgctgaaa cattagtaac gagcgcacgt caaatcaatt tctgggcgaa
      541 aaaaggcgca gttggagcag tacttgctcg tgaagttcct tttgaagaga tgaaggcgat
      601 ggaagaaaaa ctcgatattc ctgtagaaac ccttgtgtat ggtgctacat gcatccatca
      661 atccaaacgt ccactgctac agaattacta taactatacg aaacaagacg aacaaaaaga
      721 tcgtgaaaga ggtcttttta tctcggaacc taaaaaagaa gagacacatt attctattta
      781 tgaagatagt catggcacac atatttttgc cagcaatgat ctgaatctct cgaatgaatt
      841 gaatgaactt tatcagcatc attactgtac atggaaattg gacggattat ttactccagg
      901 cgaaaaattc gtaaacatca ctcgactatt cgccgaagca aaaaaacaaa ttttagaagg
      961 tagctgggat caagaaaaag cggaaaaaat gactagcgaa atcagtaaat tgcatcctga
     1021 aaaccgtgaa ttagatacag gattctttta tattgatcct tctgctatca aataaggagt
     1081 atgatgaaag aaatgacagt aaaaagaaaa ttgaaacgac cagaaatcct agcgccagca
     1141 ggaacactgg aaaagttaaa aaccgcgatc cattatggag cagatgcagt atatattggc
     1201 gggaatgcat atggtttaag aagccgtgca ggtaatttta cttatgaaga aatggctgaa
     1261 ggagttgctt ttgcaaaaga acatgatgcg aaggtatacg ttgctgctaa tatggtcacc
     1321 catgaaggaa atgaagatgg agcaggggaa tttttccgca gtttgcgtga tgtcggtatt
     1381 tctgcagtga ttgtttctga tccagctttg atcgaaattt gtgcgacaga agccccaggt
     1441 ctgccgatcc atttatccac tcaagcttca gcaactaatt ttgagacatt ggaattctgg
     1501 aaagaagaag ggttggaacg tgtcgtttta gctcgtgaag tttcaatgga ggaagtggca
     1561 gagattcgta aaaatacgga taccgaaatc gaagcattta tccatggtgc catgtgtatc
     1621 tcttattctg gtcgatgcac attgtccaac catatgtcga tgcgggatgc aaaccgtggc
     1681 ggatgttctc aatcttgtcg ctggaagtat ggattgttcg atatgccttt tggtcaagaa
     1741 cgaaacagtt taactgatac aggcgaagtg gaagaagaat tctcgatgag tgcagtggat
     1801 atgtcaatga tcgaacatat tccagaattg attgaaaacg gcgtagacag cttcaagatc
     1861 gaaggaagaa tgaaatcgat ccattatgta tctacggtgt caaatgtcta taaagcggct
     1921 gtagacagtt atatggaaga tcctgaaaac tatgtctgca agcaagaatg gatcgacgag
     1981 ttatggaaag tagcacaacg agaattagct acaggttttt actacaatac accaactgaa
     2041 aacgaacaat tattcggtga acgtcgtaaa atcccagtat ataaatttgt cggtgaagta
     2101 ttatcttatg atgaggcaac acagattgct acgatacgtc agagaaactt gttttctgta
     2161 ggagatgaaa tcgaatttta tggtccagga ttcagtcatt tcagtcaaac cgttgaagtt
     2221 atgcataatg aagaaggcga atccattgac cgagcaccaa atccaatgat gattttaact
     2281 atgccagtcg cacagccggt gaaacctgga gatatgatcc gaaaacgtaa ataaggaaaa
     2341 agctgatata tccctgtttt aagcatcttc gcatacaaaa ttcaagaaaa gtgttacact
     2401 tgttttctgt agaaaaagga tcgttaaata aaggttttga ttgatctctt caataaggag
     2461 gaacaaaatg caaatatatg attacatcgt aattggtggc ggctctggcg gtattgcttc
     2521 agcaaacaga gcagggatgc atggcgcaaa agttttgctg attgaagcga atgaattggg
     2581 cggaacatgt gtcaacgtcg gttgtgtacc taaaaaagtt atgtggcaag caagtacgat
     2641 tttggaaaca attgaacgag atgcctcaag ttacggtatc caagcagatt taaaaaatgt
     2701 tgatttcgcc ggtctagtag aaagacgaga aaaatatatt gattttctac acggtgctta
     2761 tcaaagagga ttagatagca atcatgtcga ggctataaaa ggttacgcta cttttatcga
     2821 tagtcaaacg atcgaagtaa acggtgaaac ctatcgtgct cctaaaatct tgatcgctac
     2881 tggaggccgt gcaagtaaaa tgaccattcc tggtggagaa tacgcaattg attcaaatgg
     2941 attttttgaa ttaacagagt tacctaagag agcaatcgtt ttaggtgctg gatacattgc
     3001 ggctgaactt tcaggtgtat ttcgtggttt aggtagtgaa gtcatgtggg catatcgaaa
     3061 agaacgacca cttagaacgt ttgataaaat gctttcggac aatttgatcc aaatgtacca
     3121 agaagctgga ataaaaactt acgcacatca tattgctaaa gaaattacta aaaacaatga
     3181 cgaatatacg gtgctatttg aaaatggtga aaccttaact ggtgattgtg tactttttgc
     3241 cggtggacgt gtaccgaata ccgaaaaatt aggtctggaa aatacaaatg tggaattgga
     3301 caaaaaaggg tttatcaaag tagataaata ccaaaataca acggatgaac atatttttgc
     3361 tgttggtgat gtgatcggta aattagatct gactcctgtt gcaatcgcag ctggtagacg
     3421 tttatcagaa cgtttattta atggaaagaa agattcttat cttgactata aacttgttcc
     3481 gacagtcgtc tttacgcatc caccaattgc aacaattggt ttgacagaag aagaagcatt
     3541 ggaaaaatat ggcgaaaatg aattgaaagt ttatcgttca cgctttaccc caatgtactt
     3601 tgcattaaat gattatcgcc aaaaatgcga aatgaaattg atctgtgtcg gaaaagaaga
     3661 aagaatcgtc ggcttacatg ctattggtat tggtgtcgat gagatgctgc aaggttttgc
     3721 cgtggctatc aagatgggtg caactaaaga agactttgac aatacagtag ccatccatcc
     3781 aacaggagcg gaagaattcg taacgatgcg ataatttaat attaacagtt ccaacagctt
     3841 tttattgaac aaaccatgta aaaccagaaa atcagtatac tgattttctg gttttttgtt
     3901 ttttagttat ttatgtaaga ttgcttcgat aattctcaaa acgaaataag tagggaagtt
     3961 ggagattttg attatcttcg taactgacga agagtaaatg aatccatttg gtatgcactc
     4021 ttaagttcaa aatattggta tcgatgttta aaagaaatag aaatgtatcg aagaacagat
     4081 gtttaaaaaa gtgtgacttt gtataaataa attagaagaa gcaaacgagg atgttaaaag
     4141 aataccatat agttaccaaa ccaagaaagt tctcgtcaag ttcgctggta cgaatcaaat
     4201 caagtgaaaa aagggactgt gaaaaaaatt gtgtcatagt tagtcacttt ttccgaacaa
     4261 atagtgttca atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact
     4321 atttttccat ttgtttctct aatactcagg acaaaacact gattt
//
LOCUS       DAZAFF010000154         4336 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000154 DAZAFF010000000
VERSION     DAZAFF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.162"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACG9BB_002818"
     CDS             127..579
                     /locus_tag="ACG9BB_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838672.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACG9BB_002819"
     CDS             593..1282
                     /locus_tag="ACG9BB_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838673.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACG9BB_002820"
     CDS             1407..3758
                     /locus_tag="ACG9BB_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFN2838674.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACG9BB_002821"
     CDS             3838..4233
                     /locus_tag="ACG9BB_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838675.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1526 a          548 c          867 g         1395 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttattt
//
LOCUS       DAZAFF010000155         4261 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000155 DAZAFF010000000
VERSION     DAZAFF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.163"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..766
                     /locus_tag="ACG9BB_002822"
     CDS             65..766
                     /locus_tag="ACG9BB_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFN2838676.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1338..2354)
                     /gene="tsaD"
                     /locus_tag="ACG9BB_002823"
     CDS             complement(1338..2354)
                     /gene="tsaD"
                     /locus_tag="ACG9BB_002823"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFN2838677.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2351..2815)
                     /gene="rimI"
                     /locus_tag="ACG9BB_002824"
     CDS             complement(2351..2815)
                     /gene="rimI"
                     /locus_tag="ACG9BB_002824"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFN2838678.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2822..3364)
                     /gene="rimI"
                     /locus_tag="ACG9BB_002825"
     CDS             complement(2822..3364)
                     /gene="rimI"
                     /locus_tag="ACG9BB_002825"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFN2838679.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3348..4070)
                     /gene="tsaB"
                     /locus_tag="ACG9BB_002826"
     CDS             complement(3348..4070)
                     /gene="tsaB"
                     /locus_tag="ACG9BB_002826"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFN2838680.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1258 a          914 c          735 g         1354 t
ORIGIN      
        1 actttacaaa ttctttaaaa aaagtgacaa taaatatagt gaacaataag gagaggactt
       61 tttaatgcgt gttaataaaa tcgattttca cttttcaaaa ctcagatatc aacctgattt
      121 tgaacatttc tcggatgaag agttccaaca gatcaaggaa catactgttc ttcgttctta
      181 taaaaaagga cagatccttt tcgacgatgg tgatcccaga caacggttgt atatcttgac
      241 caaaggactc gttcgtttag aacgttatga tgaaagtgca acttattact actatgacta
      301 tgtgaataca aataaccttt ttcctatgtc aggaatgttt ggagaagaga cctacactta
      361 tacagctcaa gcgatgactg atattgaaac attctacctt cctgtttcct actatgaaaa
      421 attagcacga aaaaatcccg aacagttgat ctatatgata caaaaaattt ctaaagtagt
      481 tgaaatacat gaattgcgta tccaaatcgg attgacctct agcgcctttg accgggtaaa
      541 acaaaattta ttcattttga aagaagaatt gggcgtacca acaaaagatg gtcatatcac
      601 cattccttat ccgatcacct taaaagaatt agcagtgaat agcggcacga cacgtgaaac
      661 tgctggacaa gtaatcaaac aactacgtga ttccggactg ttagattatg acaaaaagcg
      721 ttttactttc tgtaagacag ctactactcc agctatgaga gaataacgcg aacaaaaata
      781 agcagtgacc ctgatattta tcagggtcac tgcttatttt cctccctaaa acttctgaaa
      841 gatccggcag ttttagatca ctgggcaaga ctcaaagatt ttctatgtgt gtttcagtta
      901 tgtaatattc actgtttgca aaaaatactg atagattaac tagaggaagc gtcttgtcct
      961 catccgacct agattattcg atattcctcg acaataaacg attaatcagt gagggcaatc
     1021 agaggaacgg ataatgatga ataagctgta aaaaataggg ctttgacaaa agcgtgtcac
     1081 agcccttatt tcgattaaac ggtgtgtacc tgtcaactct tcggtcacaa gtcataatgt
     1141 tcataaacat gaggagctgt tttttgaaat tgttccttgt gaatcagagt taaacgacag
     1201 gcttacgaac tcttgattaa acggcgttca aaagcttgca tacgcggtaa taagaacagc
     1261 caggtcaaaa acatgagaag ccgtttttcc tgggctgttc ttattacttg atgcagggca
     1321 gatttttcac aacctcttta ttcagataaa accaagcttg gatcagcatt caactgatag
     1381 ctagcaaaat ttcttttttg catttccaca tgtgctgctg ctccaatcat tgcagcatta
     1441 tctccacaaa gtcgaagagg gggaatcact agttctacct cagggagttt agttgccaaa
     1501 gcatcctgca atccttcacg caacccttga ttggccgcta cacctccagc tacaatcagt
     1561 tgcttgactg gataattttg acaagctcga agtgttttag taatcaatac atcgatcaca
     1621 cttgcttgaa agctagctgc taaatcattt ttgtctagat cttctccacg ctgttgtgca
     1681 ttatgcacca aattgataaa tgcacttttt aatccgctaa aactgaaatc ataattatct
     1741 tcatggatca tcgctcgtgg aaaatggtaa ttatcttttc cctgatgagc tagctgatcg
     1801 atttcttttc cgcttgggta agaaagacct aatactcttc caactttgtc ataagcttct
     1861 cctgctgcgt catctcttgt ctccccaatg atttcatatg atccatcttc ttgcatatag
     1921 actaattctg tatgtcctcc gctaacaaga agtgccatca atggaaactg gaatggttta
     1981 accagacggg ctgcatagat atgccctgcc atatggttta caggaataag cggcaattgg
     2041 tgtgcccaag caaaagcctt agctgcagaa ataccaatca gcaacgagcc gacaagtcct
     2101 ggtccataag tgacagcgac tgccgataaa tcttctgccg acacaccagc ttctaccagt
     2161 gcatcttcca agcataaggt gatttgttct acgtgatggc ggctagctac ttcaggaact
     2221 acgccaccaa aacgtttatg actgtttatt tgagaagcaa cgatgttaga taagatctct
     2281 gttccatttc taacgaccgc tacactcgtt tcatcacaac ttgattcaat cgccagaatc
     2341 aattcttcat tcatttcatc gtctccgttt ttaactttct gctcataatg atcgcatctt
     2401 ccgtcggatt gcgataatat gatttccgtt tacctaatat acgaaacttt tctgattcat
     2461 aaagttttct tgctgcttca ttagatagac gtacttctaa aaaaacatgc agcgaatctt
     2521 ttatttgttc cgtaatcagt aattgtaaca aatgccgtgc atgccctttt ccttgttctg
     2581 aaactgcaac agcaatattt gtgatctcga cttcatctat tactttcgag tatccaataa
     2641 agccacagat ctggttatct tcaactaaaa gcagataatc agagtgaggc tgttggatgt
     2701 ctgccaaaaa ctgcattttc gtccaaggtg aaccatgtgg ataagcattt tcgctaatca
     2761 tccatacgca ttctgccaag tacgcttcat caaaatcttt ttttctgcaa atcaactaat
     2821 ctcactcatt ttctaaaagt tttatcatat ccaacgcatc ttcttttgtt tcagtgtaat
     2881 atcttttttt gatcgttgtt gcttgaaaac ctaacttccg ataaagtctt tgtgcatctt
     2941 gattactcat acgcacttct aatgacatcg tctcacagcg attcattata gcaaagttct
     3001 cgatttcgtc aataagcagc gaaccgattc gtttacgctg atatgccggt cgaacggcaa
     3061 tattcgtgat atggcaatct aaaccgaacg ctcttgaacc gataaaacca atcattcttc
     3121 cattatcaaa gatacctaga tatagatgag gaagcggtga cttcaattct gatataaagg
     3181 cactttttgt ccatggcaat tcgccattat agacttctcg ttcgacatcc agcagctctt
     3241 tgatatcttc tgtagtcaat tctcttagta cgtaacgacg atcgatttgt tcgattgttt
     3301 tttctgtata aatcatttta tcttgaaaaa agcttctggc atacgctcta aattttttca
     3361 acataacttt cgtctccagg tgtgtgcgtc tccaaccatt tttcttctgc ttctacccgt
     3421 tttaaatagc gaggaagaaa agcttgtttc tctgcaactg gttcagctaa agcacctaat
     3481 tgagccacag ttacaccact tggaatatcc catgctggaa ctgtattaat catcgcacta
     3541 ggcaatattt cctttatttc atagaagaat ttgtgacaat cactcccgac gaaataaata
     3601 gcttcattca gcttttgtag ttgtgtgaac aaatcagaag cagaaatatg tacatcttca
     3661 agtacaattt ctaatctatt ttctttccag cggtatgccc cagcgtatac attttttctt
     3721 cgagcatcaa acatgggcac aataatttta tccacaccga tgcaattagc cgcgatggtt
     3781 ttcagactag atactcctac tagctcttta tctaatgtat aagcaatcgt ttttgctgtc
     3841 gttacaccta tacgaagccc tgtataagaa ccgggaccat cagaaaccgc gattcggtca
     3901 atgtcttttg gacttatttg aagatctgca aacaattggt cgattgcagg catcagtgtt
     3961 acactgtggt tgcgtttgat atttgtttgt atttgtccaa gaatttgttc gtcttctacc
     4021 acaccgatcg ccaaagtttg gtttgcagta tctattccta atgtaatcat atatttgatc
     4081 ctttctgcta tgacattctc gccttccatt gtagcatact tttttattct gaggatgaaa
     4141 ataccaaagg caaaactagt acaatgatac atgattatct gctggcgggc aaattttgcc
     4201 ccttcctgaa acacaaaaaa gctgcggtat cacttcattt ccccacagct tttccatctt
     4261 t
//
LOCUS       DAZAFF010000156         4066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000156 DAZAFF010000000
VERSION     DAZAFF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.165"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACG9BB_002827"
     CDS             85..1434
                     /locus_tag="ACG9BB_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFN2838681.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACG9BB_002828"
     CDS             complement(1562..2152)
                     /locus_tag="ACG9BB_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFN2838682.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACG9BB_002829"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACG9BB_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFN2838683.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1172 a          744 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DAZAFF010000157         4065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000157 DAZAFF010000000
VERSION     DAZAFF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.166"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1155)
                     /locus_tag="ACG9BB_002830"
     CDS             complement(<1..1155)
                     /locus_tag="ACG9BB_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2838684.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
     gene            complement(1158..3407)
                     /locus_tag="ACG9BB_002831"
     CDS             complement(1158..3407)
                     /locus_tag="ACG9BB_002831"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFN2838685.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3504..4007)
                     /locus_tag="ACG9BB_002832"
     CDS             complement(3504..4007)
                     /locus_tag="ACG9BB_002832"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2838686.1"
                     /translation="MENSIYLVTEPNTQLKDQDNLVSELSRQKQVYKFLELLLNSASY
                     LNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIK
                     EYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLG
                     LGIINGE"
BASE COUNT         1310 a          743 c          580 g         1432 t
ORIGIN      
        1 tctagtattt tttttatttg tcgcaaaaaa tgttacaaat aggatcacaa taaatattcc
       61 tgcgaataca aaaaatggag cagtcaaatt tgtacttctt gttagaaatt gtgctaattg
      121 catagcaata ggagctataa aattaccaat attacagcca ataaaaatca atgaagtagc
      181 aaaattaatt gtattctttg tagtgacttt atctaagtta ctaaagataa atgggaagca
      241 ccatgatcct gttatacttg ataaaaataa acccgttagc aggaacgtca tattaccgtt
      301 tgacacagca attaacaaat ttgaaattat aaagaatata attcccaagt atagggttcc
      361 ttttcctatc cacttattta ttggtccaaa cataaatcct gctagtattc ctaagatagg
      421 cattagagct aaatagttac tagcattaaa gtttacccct ttaatttcag ttgcaattga
      481 tgcaaatcgt acagaaatag caatactatt cattacgagt aagattgcaa atagaactag
      541 ggcaaaaacc attggattca tcttttcttt tacatgattt tcaggtatgt cactattttc
      601 atctctaatt tcaggaactt ttagcgcgaa taagagagct acaggaaacg caattgcata
      661 gactaaaaac gaagccgtcc aattaatgtt cactaaaact ccagcaagaa aaataaggat
      721 tgtctgacct aaagcttcca ttgaatttct aatacctaat agcgttgctc tagtattgcc
      781 agagtataaa gagctgatat atgtcacagc caatgaatta tatactccta aaccggcacc
      841 taatattact cgactaataa gaacggtttg gaaggtagct gtaaatactg gaataattcc
      901 tcctatacca gctaataata aacctaaaat tattgtcttt ttcattccta ttcttttggc
      961 aaagtaacta gataataaga taaacaaaat aacagttata cttggtgcag ttcctaaaag
     1021 ttcactttga ctttgtgata tccccaaaga ctgtttcatt tgtggaataa caccattaat
     1081 tgcttgagag cttgttaaca ttagtgaaat acttaagatt gctccttttt gaagtaagct
     1141 tttttggtta ttcatctcta cttttcctcc tgtgttataa cccaaatctt ggaatcaaaa
     1201 tcaaatgttt ctgggcgtcg attctgatca tttatttgtc cactagatgc atctgaacag
     1261 actagtcctg ctctcatcaa ttccattcca gatcgcaaac tattttgtcc tacattataa
     1321 agtccgtcat tatctaaccc ttttaatttc agtcggcggt aaggagcatt tacctcattt
     1381 aatgttttat aatgagcaac aatagccgtt ttcttatcat gactaacaac catccacgcg
     1441 gtttgattat catcatcgaa tggtgataat agtctataaa aagtaccatt atgaattatg
     1501 gttctatatt ttttataaaa ggcaatttgt tcttttacaa tcctcaaatc gccttcactt
     1561 aattcattta agtcaagctc atatccaaat gcaccaaaaa aagctacatt tcctctagtt
     1621 tttataggtg ttatacggtt tgtttgatga tttggaacaa ttgatacatg agcaccaata
     1681 gacgaaagag gatacagcat actagttcca tattgaattt ttaacctttc aattgcatct
     1741 gaatcatcac tagcccaagc ttgaggtgca tagtgaagca ttccaggatc aaagcgccca
     1801 ccacctgatg aacaagactc aaatagtatg ttaggatatt ttgttataag tttttcatat
     1861 aagcgatata cgcctagtac atatctgtgg aatacttctc cttgttgatc tgctgacaag
     1921 tgactgtcaa atacatcagt caatggacga ttcatatccc acttaatgta atctaagttt
     1981 gattcatcaa tgattttaca catttgatcg aaaatatttt caacaacctc atctaaccca
     2041 aaatttaata catattggtt cctaccatga ttaggttttc gattaggtgt agaaataatc
     2101 caattcggat gtgtgcgata taactcacta tccttattca ccatttcagg ttcaaaccat
     2161 aaaccaaatt tcataccaaa actacgaatt ttttctgaaa ggctgccgat tcctctaggt
     2221 agtcttttag gatttacatg ccaatctcct aatcctgcac aatcgttttc tctttctcca
     2281 aaccagccat catctaatac aaataattca ataccaacat caaatgcttt tttagcaata
     2341 tttaccaact tttcttcgtc aaaatcaaaa tatgttgctt cccaattatt aattaagaca
     2401 ggacggtctt tttctcgcca cttaccacga gctaagcgtt ttctaaatag ttgatgaaaa
     2461 gtttgactca tggcatttaa acccttactt gaataaacca gaatcgcttc tggtgctgta
     2521 aatatttctc ctggattgag tttccaatta aatccaaatg gattgatgcc aacttgtagt
     2581 ctagtaacat cccatgtatc tacttctgct tgaattaaga agtttcctga gtaaactagt
     2641 gctgcaccaa tagcttctcc ttgattttca tcagtatttt ttctttttaa agtaacaaat
     2701 gggttatgct gatggcttga aatacctcga gtagattcga ttgattgaat tccttgttgt
     2761 aaagtacgcg acttaatagt tctttctcta ccccaagcac cagataattg ttgccattca
     2821 taatttgcat cgggtaaatc caatgataaa ctgctaatat tctcgattat ttgaatatct
     2881 tttcctttat tcataactcg cgcgctacga gtaataacag gataatctct ataaattgta
     2941 tataataatt ctacttgaac atttgtaact gaatctaaaa tataaacttt caatgttttg
     3001 ctttcatcat tattatcaac ataggtagca ggtaaacctt ctagtttagg ttttccttct
     3061 tccacttcgt acccttgata gacaaaatct gataagcgag agccattttt ctgtagtaat
     3121 gaaatagcag gatgtctaaa atctgttgta ccatattcag gaaattcttg ttttaagtgc
     3181 tctaacgaat atagcaaatc gttttcattt ctataggttg tcataggccg atgatgcatc
     3241 tcaattagat gactatagtc attatctggt aagttctcac cataatataa atgcaataat
     3301 ttaccatctt ccgatatctt aaaaatgtaa cttatctcct gattaaacaa atgaaattgt
     3361 tggttttctg atcgataaat aatattcatt tcattatcct tatacataag ctctcctccg
     3421 tttctttatt gcattataaa aaacgctcac ataaatagta agcgtttaaa gtaacgaata
     3481 tttatcaaaa tcaaatgttt agtttattca ccattaatga ttcctagacc taatctacgt
     3541 tttcgatata ctcccggtga aacaccattt gctttcttaa aggctttaga aaaagattgt
     3601 actgttgaga atcctatctt gtcactgatt tgctggattg aatattcagt tgatgataac
     3661 agatcgtttg caatagaaat tttgatttca ctaatatact cttttattga tattcccata
     3721 tcattcttaa acaatcgtga taaataattt tcgtgtatcg atagactgac agcaatatct
     3781 ttaacagata atttttgata cgcattctca cgtattattt caattgtttg acgaacaaaa
     3841 ggattcatct ttttagtaat tatcccttcg ttcagataac tagctgaatt taagaggagt
     3901 tctaaaaatt tataaacttg tttttgtctg gacaattcac tgactagatt gtcctgatct
     3961 ttcagctgag tgttaggttc tgttactaaa tatatagaat tttccatatc caccctccta
     4021 tgttagattt ggataatttt tgttttatta tgtttatatt attac
//
LOCUS       DAZAFF010000158         4041 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000158 DAZAFF010000000
VERSION     DAZAFF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.167"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1655)
                     /locus_tag="ACG9BB_002833"
     CDS             complement(150..1655)
                     /locus_tag="ACG9BB_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN2838687.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1652..2338)
                     /locus_tag="ACG9BB_002834"
     CDS             complement(1652..2338)
                     /locus_tag="ACG9BB_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN2838688.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2531..3952)
                     /gene="gndA"
                     /locus_tag="ACG9BB_002835"
     CDS             complement(2531..3952)
                     /gene="gndA"
                     /locus_tag="ACG9BB_002835"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFN2838689.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1113 a          855 c          692 g         1381 t
ORIGIN      
        1 ctaaaagata cttacattta attaacggat gttattctat ttaattgttc gctagttttt
       61 ttataatgac atccctgaat ttctcacttg cttgtatttt gtaccttatt ctttgttgga
      121 tatataaaaa aacaagtaaa gcacatgctt tacttgtctt ctgcctttgc gataggaata
      181 aagattctaa aaattgtccc ttgccctact acactttctg ctaagatacg tcctttatag
      241 ttttcaacca gttgtttagc gatagacaac cccagaccat ttccaccctt cgttctagct
      301 cttgctttat ccactcgata aaaacgattg aagatcttat taaggtcttc tttgggtatt
      361 ccttcgccaa agtcctgaat cgcgatctca aattcattca gagtggaaga aatagagata
      421 tggacttctt ttcgtgtagt tgaatacttt actgcgttat ccaatatgat aatgattaat
      481 tgttcaaagt gattacgata aatttgcagg gtcacttctt ttgtcaaatc atcatctaaa
      541 gtgatcacat agtctggata taatatcttg aaattattat aaacttgata ggtgacttct
      601 tttcctttag ttgtttcatt cgcataatga acatctactt gttctgctct tgataagtct
      661 agcatctctt gaactaagct tttcatacgg ctgatttctt gcatgctggc gcttaatgat
      721 tcttctaata tctcaggatc atctttcccc caacgattta gcagactaag atgcccttca
      781 ataattgcaa caggtgtcct taactcatga gatacgtctt ccacgaattg ctcttgttgt
      841 tcaatataca aacgcatacg gtcaagcatc tcattgaata tttcagctaa atctgccaat
      901 tcatcatttg tgtcgatttc aggcatgtgg atatctgatt gcggatcttt acgtatagta
      961 tccattgtat ctctgagtac cttgagtggt tttaagaaat aagaagataa aaagaaacct
     1021 aaaatactac ttaagatcaa agaaacaact tctaaaacaa taagagtcaa taatagctta
     1081 tttcgaatat catagaaaga agataattca tagaagcttt gcgcatagcc gatcttttct
     1141 cttgtacctt tagaatatat aggctggata gataaaaagc cagtttttcc ttctagtgta
     1201 acgatcgttg gaactttacg tgtagtctga atcaggtttc gactgttttc atgggtttta
     1261 aaaatcaagt tctcgtccaa atcataaaca taaagatcca ttgatggctg ccctagctca
     1321 gctataaagg gatcgatctt cattaagttt ccttcgacca gtgaagtatg atctacgacc
     1381 tcatctccgt catttgtcgc tttcgtcaga ttacgatacg tatttactaa tgtcagttct
     1441 tcatccgagt tagccaaacg agaagctact tctgaaatcg tacgctctac attgtttctt
     1501 tcttttgcca caatcagatt gatagatgat ttataggtaa tgacagcgaa aattgtaaac
     1561 acaacgaata tgaagaaaga actcgcaaag gcccacttga ttgtcaagga gggccctttc
     1621 agttctcttt tttccgattt tttcggcttt atcacgagcg catcacataa cctgttccgc
     1681 gtacagtttg gatatagctt tcctccccgg gaacgtcaat tttgttgcga agataacgaa
     1741 tatatacgtc cactacgttt gtttcaactt ctgtttcgta tccccacact ttatttagta
     1801 aaacgtcacg tgctaatacc acgttcacgt tttccattaa agtaagcagc aactcgtatt
     1861 ctctttttgt tagttcaatt acttcatttc cgcgacgtac gacacggttt tctttttcga
     1921 tagtcaaatc gcgataagta atagtagttt gtttagccac atttttatcg ccttcaatat
     1981 cgattcgacg caacaatgca cgaagacgtg ctaataattc ttcgatagca aatggtttga
     2041 cgatgtagtc atctgctccg tggtcaagtc cagatacacg atcgatcaca gaatctcttg
     2101 cagtcatcat aatgattggt gtatttttga cttggcgtac acgtctgcag acttctaaac
     2161 cgttcaattc tgggagcatc agatccaaca atatggcatc ccactcatta tttaatgccg
     2221 catctaaacc agttcggccg ttgtagtgga cttctgcgtt atacccttca tgcttcaact
     2281 ctagctcgac aaatcgtgcc aagttctttt catcttcaat gattagaata ttgctcattt
     2341 attattttat cctttctatt ctactggctt tttaaaaact ctatcattat accgataata
     2401 tgcttaaaaa tctatagtca ttttatcatg gatagcaatt ttaaaaaaga gctgaagtct
     2461 actcataaca atcgacttca gctctttcgt cctcttttta ttttctcatt tcctaacagc
     2521 cgaaagacta ttattcttca tggtaccatg agtaatggaa gattccttct ttgtcgacac
     2581 gctcataggt atgtgcgcca aagtaatcgc gttgggcttg gattatattt gcaggcagac
     2641 gttctgctct gtatgaatcg aagtaagcga tggctgatga gaaagtcggt acaggtacgc
     2701 cagcttggac agccaaggca acgacgtctc tgactgattg ctgatatttt ttcgtgattt
     2761 caacgaagta atcatctagc aataaatttt cgatctcagg atttttgtca taggcatccg
     2821 taattttttg caagaatcga gctcggatga tacatccagc gcgccagatt tttgcgattt
     2881 caccaaatgg caagtcccaa tcaaattctt ttgaagcgac acgtaattga gcaaatcctt
     2941 gggcataact catgatttta ctgaaataca acgcttctcg gattttttcg accaattctt
     3001 ttttgtctcc cgtgaactca aaatcgttcg tacgagacaa aatcttgctt gcttgtacgc
     3061 gttcttcttt ataagcagaa atatagcggg caaatacaga ttcagtgatc aatggaagtg
     3121 gcacacctaa gtcaagcgcg ctttggcttg tccatttacc tgttccttta ttcccagctg
     3181 catcaaggat cacatctact actggtttac ctgttccttc atcgtcttta cgtgtcagaa
     3241 tgtcagcagt gatttctatc aaatagctgt ctaattcgcc ttgattccat tctttaaaga
     3301 tatcagccat ttcttctact gaaaggttta aaatattctt cattaagtca taagattcag
     3361 caatcagttg catatctcca tactcaatac cgttgtgtac cattttcaca taatgtccgg
     3421 ctccatttgg gccaatatat gtgacacatg gtgctccgtc ttctgctttt gcagaaattt
     3481 gctctaagat tggtgcaact agcgcatagg cttctttttg tccaccaggc atgattgaag
     3541 gtccttttaa agctccttct tcaccaccgg aaacgcctgt accgataaag ttgatgccag
     3601 agttggctaa ctcttcatta cgacgcatcg tatctttaaa gaatgtattt cctccgtcaa
     3661 tcaaaatatc gcctttatca aggtgaggaa gtaactcttg gattgttgca tctgttgccg
     3721 gtcctgcttt gaccattagc aagatccgac gtggtttctc gatagattct acaaattctt
     3781 cgattgtata tgttgctttg aaatttttat ctgggtgttc ttctactaca gcggttgttt
     3841 tagcccctgt acgattgaat aaagccactg aatagccgcg gctttctatg ttcaaggcta
     3901 gatttttacc cataacggcc ataccgacaa cgccgatttg ttgttttgac atatgaactc
     3961 ctcctacata cgggatttct ccctaatctc ttacattata gcttaacacg gctacattgc
     4021 aaactaaaga acaacatatt t
//
LOCUS       DAZAFF010000159         4009 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000159 DAZAFF010000000
VERSION     DAZAFF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.168"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..615
                     /locus_tag="ACG9BB_002836"
     CDS             <1..615
                     /locus_tag="ACG9BB_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFN2838690.1"
                     /translation="NLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLG
                     MDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQ
                     FIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNH
                     TISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            632..1066
                     /locus_tag="ACG9BB_002837"
     CDS             632..1066
                     /locus_tag="ACG9BB_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838691.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1683..>4009
                     /gene="esp"
                     /locus_tag="ACG9BB_002838"
     CDS             1683..>4009
                     /gene="esp"
                     /locus_tag="ACG9BB_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFN2838692.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1465 a          561 c          765 g         1218 t
ORIGIN      
        1 aatttagaaa acatggtttt taatctgagt aatggaataa ttccaagtgt tagtggagat
       61 acgattcggt ctgaaaaaaa ttattctatc attgtttttg aaaagttagc acaaacgtct
      121 ataacattag gaatggatat aatagaagcg tatcaaagta gagatgcttt gatacaagaa
      181 aacgaattag ctgtatcgtt acctgaggtc ttaaaagtga gggattctgg gattgtttat
      241 tatactaagg aaatcggaaa aactaagata gagcatcttt ctcccttaat ttcttctgtc
      301 gttcaattca tcggcttaaa tatctataaa agaattactg taaaggaaat agctaattat
      361 ttttctgtaa gtgaaactaa attaagagaa agctttaaga atgaaatgca tattacgatt
      421 tataattata tctctaagcg aaaaatttct acggctaaaa taatgttgaa atcaaaccat
      481 actatcagtg aagtttcttt aggtttagga ttttcagatt cttcacactt ttcgagagta
      541 ttcaaaaagt acgcaggtgt atctcctaag caatatcaat taggtttagt ggacaatttc
      601 aacaacatta gctgagaggt tttattttat tatgagacta tatcatttac gtagtttgga
      661 ggagttgagg aaattggatt ttaacaaaaa atttttttgg gaaatttctg atttgagttg
      721 tttcgtagaa tctgagcgtc taagtaatgg taataatagt gtgttaaatg aatatgacaa
      781 agtagtaatt aataaagtaa aaagtttatg tgaagaagct agagaatcta tcagtcatat
      841 taatggtagc aattttaaac aaacattatt taatctgatt aagctagatg ctaaaatatc
      901 aagttattta ttctttttga tacatgatga atttatggat tatcaaaaat tggatcagtt
      961 aattgaaaga gaaagctggg aagattatta cgtgggtcta actttttcag aaaagttaaa
     1021 caactacaag ttaatagatt ataagtattt gagtgaaagc aattaattat tcatgtagag
     1081 tactagacac tttaaatatg gacaattcaa ttataaagaa aaagaatcaa taatttaatg
     1141 aggctgtgac aaatgtcatt ttttgtttcg ctaaaaacga gtgaaaaaat gttgataact
     1201 ccaatcattc gtttaagatt ctaggaatca ttcagaaatg ctgtgaaacc gatactttat
     1261 aattataaag aaggtgattt caaattccga agaaggaatt tgaagtcacc tttttactta
     1321 tgtcacagtc tcattcagta atgcgggata aggctcctta ttccgttatt tgaatactct
     1381 gaagtttaat ctaagaaagg gtaatatttt gaaaacatgc ttcatcgcat agaaatgaac
     1441 ttaatttcag aaagaataca tacatatctt tttatatgtt ttcagctgct gaatggttaa
     1501 aaagcacaaa tatccgaaaa aaacatgaag aaatgtactt tttttgttgt aatgttcatg
     1561 aaattctcta agtaaattag ttaacataag gttagtaaga ttcctattct gtgaataata
     1621 cgtatcagat ttatctttta ttcttactag gaaagtaatt attaagggag ttgatttgaa
     1681 taatggttag caagaataat aagagagtat ttctagaaaa aacaaagaaa cgagtattga
     1741 agtacagtat caaaaaatta agtgttgggg ttgcatctgt tttgattggt gttggtcttg
     1801 tcttgggaac aactgaacta gttaaagcac aagatgaaat ttctccaagc agcccacttg
     1861 aaacagcgat aagctccgta caagtaggtg acaaagtagc ttcggggaat acttttcagg
     1921 agaatcctgg ttatacgaaa aattataact tttcggattt acaatttagt cctcaagaac
     1981 taactggaga cacactaaaa ggcaatacaa ttggatttga agtttacgga aaacataata
     2041 ttgcagcatc aactaaaaac tgggaaatcc gtcttcaatt agatgaacga ttagctaaat
     2101 atgtagaaaa aattcaggtc gatccaaaga aaggaattgg atcttctaga cggacctttg
     2161 taagaattaa tgattcgctg ggtagaccta caaatatttg gaaggtcaat tatattcgtg
     2221 caagtgatgg attatttgct ggggcagaaa caaccgatac gcaaaccgct cctaatgggg
     2281 taattacgtt tgaaaagagc ctagatgaaa tttttaaaga gataggcata gacaatctca
     2341 agaccgatcg tttaatgtat cgaatctact tggtaagtca tcaagacgat gataaaattg
     2401 taccgggtat tgatagtacg ggctactttt taacagattc agatgatttc tataatagct
     2461 tagatgtatc tgaaaataat cctgaccagt ttaaacatgg atcagtaagt gcaaaatatg
     2521 agaaacctaa tactcaaaca aaagatggtt caggatccac aggtgctaat ggagctataa
     2581 tcttagatca taagttaacg aaaaattata atttctcata ttcatcttcc gcaaagggca
     2641 cgccttggta tgctaattac aaaattgatg aacgattagt tccatatgta gcgggtatac
     2701 agatgcacat ggttcaggct gacaaagtaa catatgatgt ttcgtttgaa tccggaacaa
     2761 aagtagctga tttagcaatc gagcgacggg aaggtcatga aaattatggc ataggttcaa
     2821 taactaataa tgatttaact aaacttatcg attttgctaa tgcaagtcca cgtccagtcg
     2881 ttattcgata tgttttacaa ttgactaagc cattagatga aatcttagaa gatatgaaag
     2941 caacagcaca agttgaagaa aataaaccat ttggtgaaga tttcatcttt gattcttggt
     3001 tgtcggatac gaataaaaaa ttaatccaga acacttatgg aacaggttat tattatttgc
     3061 aagatattga tggtgatgga aaccctgacg ataaagaaga gagcggagac acgaatccat
     3121 atatcgggaa acctgaatta gaagaagtat atgatgttga cacaacagtt aaggggaaag
     3181 tattcatcca cgagttagcg ggaacaggtc acaaagccca acttgttgat aaagaaggta
     3241 ctgtattagc agaaaaaact atcgctccaa atgaaaaaga tggggctcca atttcagata
     3301 ctgtagaatt tgaatttacg ggtgtagatt caagtaaatt aatcgcgaaa gatgaattaa
     3361 aaatccaaat cgtttctcca ggttttgata aaccagaaga aggttcaacc gttattaagg
     3421 aatcaccaaa agcggttgat aaacaaaccg tggtagttgg atttaaacca gatgctaaag
     3481 aatcaattcg gaataataaa aacttacctg aagatgcaga gtattcatgg aaaacagagc
     3541 ctgatacttc taacgttact gatagtacga aaggtattgt aactgttaag atcggaaatc
     3601 gaactttcga cgtggatgta gagtttgctg taaaagcttc tcaagctatg gaaaatgatg
     3661 caacatacgt acctataaca acaactccag aaacgacagt tcaaagtgga aaacctacat
     3721 ttgataaacc agatgttcct ttagctaaag atgccttttc aatttcagat gtctataata
     3781 agtacttcgg taatgcaagt gttgatgcaa acactggtat tgttacattc actccagcta
     3841 aaggtgtagg agaatcggag ccgattactg gaacaattcc tattaaaatt gtttaccaag
     3901 atggttctgt aggcacgacc gatttagcag taactgtaag taaagatatt tatgaaaatc
     3961 caggagaaaa cattcctgca ggctaccaca aagtaacctt caccgcagg
//
LOCUS       DAZAFF010000160         4008 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000160 DAZAFF010000000
VERSION     DAZAFF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.169"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..135
                     /locus_tag="ACG9BB_002839"
     CDS             <1..135
                     /locus_tag="ACG9BB_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN2838693.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACG9BB_002840"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="ACG9BB_002840"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFN2838694.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="ACG9BB_002841"
     CDS             complement(2412..2663)
                     /locus_tag="ACG9BB_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFN2838695.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="ACG9BB_002842"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="ACG9BB_002842"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFN2838696.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="ACG9BB_002843"
     CDS             3613..3831
                     /locus_tag="ACG9BB_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838697.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1119 a          850 c          735 g         1304 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caagaagtta aacttttacg ggggtgtgac aaaaatcggt
     3901 cacatccttt ttttcgaata aacggtgttc taaagccagt ttttcggtat taagtcaaat
     3961 tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttcc
//
LOCUS       DAZAFF010000161         3932 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000161 DAZAFF010000000
VERSION     DAZAFF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.170"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..1401
                     /locus_tag="ACG9BB_002844"
     CDS             10..1401
                     /locus_tag="ACG9BB_002844"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFN2838698.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1417..2457
                     /locus_tag="ACG9BB_002845"
     CDS             1417..2457
                     /locus_tag="ACG9BB_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN2838699.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2476..3561
                     /locus_tag="ACG9BB_002846"
     CDS             2476..3561
                     /locus_tag="ACG9BB_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFN2838700.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3594..>3932
                     /locus_tag="ACG9BB_002847"
     CDS             3594..>3932
                     /locus_tag="ACG9BB_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFN2838701.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKN"
BASE COUNT         1419 a          550 c          725 g         1238 t
ORIGIN      
        1 agtgtatata tgaataaaaa tggagaatgg agtgctgcac gacggatatt cattatatta
       61 atggatgtta ttgtatacaa tgcagcaata tatagtagtt tcttactgaa gtttcgtgga
      121 gagatcccat tacgaaattt agaaactttc caacactcag caattattat ttcagtgatt
      181 tttattgcgt tgaacatttt gttgggtgca tatgtttttt ataatcgaat gatcagtgac
      241 atcgtgtttg tcacagtcat cggtcaggta ttgatgacac tagggatcat ggtagtgact
      301 tttgcgggcc gctggtttgc ttttccacgt cttgttatct tactttcatt tgttctcggt
      361 acgcttttct taagtatcta tcgtattctc gtatataaac tttatttacg cgtaagtcga
      421 gataaaaagg tcgtcattat tgggttagaa aaggatgtcg caccagcaat ccaaaatttt
      481 ggttcaaaga aaaacaacaa acataaagtc gaagcagtca ttcttgataa ctattatgca
      541 aacatcaaaa atatgattaa agacatcgat attgtctatc ttgcttctca tatcgaagaa
      601 actgaaaaaa tcaaaatcta tcaattagtt actaaaaatg aaaaaaaatt gtttttgaat
      661 acaacatttg aaaacttgac attgatcaat ccaaatatta tgaatttcga agatgaaagt
      721 attattgaag catctggatt tagaatccaa cctgaatatg aactattcaa acgtttgttt
      781 gatatcatcg tttcacttgt tttattagtt attgcttcac catttatgtt attcacagca
      841 attttagtca aagtgacatc accgggacca atcatctata aacaagtgcg gatcacaaaa
      901 aatcaaaagg aattttcaat ctataaattc cgctcgatga ccgccacagc cgaagcaaaa
      961 tctggaccag ttttagctaa gagcaatgat gcacgagtaa cgcctgtagg taaatttatt
     1021 cgagctgtcc gctttgatga attaccacaa atcttcaatg tgctgaaagg cgatatgtcg
     1081 atcgtaggac cacgtcctga acgtcctttc ttcgtcgatc agttcaatgc agaaaaccct
     1141 tattactact tacggcataa tgttcgtgct ggtatcactg ggtatgcaca agtctatgga
     1201 aaatatgtct ctgattacaa cagtaaatta cgttttgatt tactttatat caaaaaatat
     1261 tcattgatgc ttgatttaaa gatcttgctg caaaccatca agatcctatt tgataaaatg
     1321 tcctctcaag gacttgaaga agacgaaaat gtgatctcat tggcagaaat tgcttttccg
     1381 aatgaaaaaa tctttcgcta gttgggaggc gtaaaaatga acaatccgaa attcagcata
     1441 atcgtaccag tatacaatgc agaagcaact attggtcgaa cgattgatat tctaaaagaa
     1501 ttggattatg aagagtacga agtcgtttta gtaaatgatg gatcaacaga tcaaacacag
     1561 gaaatcattc aagaactgat tgaaaatgat gaacgttttg aattgattac tacgaccaat
     1621 caagggcccg gattagcaag aaacgaagga ataagacagt caaaaggaga atatctctta
     1681 ttcttcgatg cagatgacac gccagtaaaa acgatcttgt cagattatga tcatttgcta
     1741 gaaaagagtc caaaagcgga tcttgttgtt agttcgtttg tttttcgtac aatggacgag
     1801 ggaagaatcg tttccgaaaa agaaaactta gttacagaat gtcactatct aactcacgaa
     1861 gagtttataa aagacatgta tcagttaatg aatgaacagt tgatgtatgt tgtatggaac
     1921 aaatgctatc gtagcgatat tctgaaaaat aaccaaattc tatttaaaac ttataatagt
     1981 tgtgaggatc gaatatttaa cttacattat tatgaatact gtcatcaagt gatcatgaac
     2041 ccaaaagtag aatatattta tgaatttgaa ggtgggaagg ggatcactaa tcaataccga
     2101 cctaacaagt tcgaaacatt taaagaattc tattcattag caaatactgt aacagacgat
     2161 attaataaag acggtatggc ggcgcttctt ttaaaaggga caacttcagt tattttttct
     2221 atttatagta cctctgcaag aacttcaaaa gagaagaaag acgaagcaaa gcagatattg
     2281 agtgatccag tgatccgaga agccaagcaa attgctaaga cggattcttc agcaaaaaaa
     2341 ataacaaagt tgctttacaa tatgccagct ccactatttt tatctgctgt aaaagttgga
     2401 agcttcgttg aagtgaaaat gccagggtta atggctgctt tgaagcgagt atattagtta
     2461 atggaggaaa atataatgaa aagaattttg cattttcagg gacgaatggg tttaggtgga
     2521 gcagagtctt tcatgatgaa tttatatcgt aaaattgatc gtacaaacta tcaatttgat
     2581 tttttgattt atgaagatta tgcagatgtt caagactatc attcagagat tgaaagatta
     2641 ggaggccgta tttttgttgt acctaatcct aagaaaaata tttttaaata tttaatagaa
     2701 gtgaataagt tactaaaaaa agaatctttt tctatagttc acaatcaggt ttattttggc
     2761 gggggaatta atttatggtt agccaaaaag aatggaatca gacagagaat tgctcatagc
     2821 catgcaactg aagatggaaa atctcaaaat attgtaatga atgtattgcg taaattttta
     2881 accaaattgt tgttacaaaa tgcaacagac taccttgccg ttagtcaaga agctggtgaa
     2941 agtctgtttc aaaaccaccc ctttgagata gttcataatg gtattgattt agagctttat
     3001 agtaaaaatt cagaagctaa ggttaataag agaaaagaat tggatatctc aatgagtact
     3061 tttgttgtgg gaaacattgg acgattagag aaacaaaaaa atcaatctta tttgttagag
     3121 atttttttag aaatactaaa aatgaattct gattcttact tgattttagt aggtgaaggg
     3181 tcacttagaa ataaactgaa agccaaagca gtagaattgg atatagagaa aagagtaaaa
     3241 tttttaggag aaagaaacga tattcccgag ttattatctg tgatggatgt ttttgtaatg
     3301 ccttctcttt atgaaggtct gccaatggtt ggattagaag cacaagcagc aaaattaaaa
     3361 ttagtcttag cagatactat ttccaaagat acaaagttaa caaataatgt ggattttgta
     3421 tcattagatg aaacaccaca agaatgggca aaaaaagtaa taaaagagcc gtatgctaat
     3481 aaaataacac aagaactaaa gagttatgat gtttcgtaca ctactgaaca aatggagagt
     3541 atatatagta gagatcatta aaaatgttca agatatttta ggagtgatta tttttgaaaa
     3601 tatgttattt gatattggca tacaatgata ttggcaactt aaagagattg gtcaatcgat
     3661 tgaacgaaaa tgcagttttt attattcata tagataaaaa aactgatata gaaccattta
     3721 ttgaagcatt taaatcatat gataatgttg aatttttatg taataaaaac cgtgttaaaa
     3781 tttattgggg tggtttttcc attgtacaag cagaaatcaa tttagtaaag agagcattac
     3841 aaaatgagaa atatttaaaa tatgtattat tatctggtgc tgattatcca atcaaagata
     3901 atgagtatat ttataattat tttaaaaaga at
//
LOCUS       DAZAFF010000162         3867 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000162 DAZAFF010000000
VERSION     DAZAFF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.171"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..573)
                     /locus_tag="ACG9BB_002848"
     CDS             complement(31..573)
                     /locus_tag="ACG9BB_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFN2838702.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            708..1097
                     /locus_tag="ACG9BB_002849"
     CDS             708..1097
                     /locus_tag="ACG9BB_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838703.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1101..1865
                     /locus_tag="ACG9BB_002850"
     CDS             1101..1865
                     /locus_tag="ACG9BB_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN2838704.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            1994..2941
                     /locus_tag="ACG9BB_002851"
     CDS             1994..2941
                     /locus_tag="ACG9BB_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFN2838705.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3071..3811
                     /locus_tag="ACG9BB_002852"
     CDS             3071..3811
                     /locus_tag="ACG9BB_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838706.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1277 a          663 c          798 g         1129 t
ORIGIN      
        1 tagatattag acagttttta agtttgtgag ttattttcct tgtcgttttc tcactttttt
       61 agtccagaaa ccacctgaaa tatattttgg ctcataagaa atgataaaag cacgctcttc
      121 gatttctttg atcaggcgat acagttcacg ttcgcttttt ctaggagaaa gtatttcgag
      181 aactagccgc tcaccttcca gccccattgc agaagtctgt gtcacaccat aaccgtgttc
      241 tctcagtata cccggcaaac ttttatcttc tgcgctattt gctggcaaaa tcgttgtgac
      301 catgatgtat cctaatgcca atttgtcttc aatcaaaatt ccgacactga tacctactgc
      361 gtaacctaac gcatagacaa ataaattcag tggattatct aaacgatcta aaacaaggga
      421 taagccgacg atataaatcg tgatctcgac catactgaca agtggagcaa tcagtcgata
      481 gcctttcatt gtcaacatga aacgtaacgt gttcagagtg atataagcaa aattaatgag
      541 gaagatcatc cccaacattt ttggatcaat catttgtaaa actcctttca tttatataac
      601 aacaatatca aaaaaaatcg taagtgaaga aaaattgaaa ctttatacat acaaaaaaat
      661 gataaaatga ttataataga attataaaga taggttaggt gattaaaatg aaaacggtca
      721 atcgtgtgtt ttgtgccagc cacgtgctaa gtctccttct cagtatatac ttaggtgtct
      781 attcgattca acagccaatt gaggtagaga acattgtttt tcagatgtcg ctgacagatg
      841 gtttaatttt aagcgttttt ttctttttga tactgtttat tggtaatatt ttcggtagcg
      901 ttgccagttt tttgaatttt tctatttatc cactcctttc gctagcatta ggagtggtgg
      961 gactttgttc tctgtgctta tttcccgagc cattcagtcc tgcatttata ttatttggta
     1021 cactgaacct gttccagaca actgttggag catggttgct atggcgatca ggaaatttaa
     1081 tgaaaatagg tgagtaacaa ttgaatccaa ttatattgaa acgttctgtc catcatattc
     1141 cagtgttaga agttgttctg aaagaattaa gagaggaacg tattcccgtt gtcatctatt
     1201 atcacggatg gcagacaagt aaggagttag ttttgacgca aggaagaaaa attgcgcagc
     1261 agggcattcg tgtgatcctg ccagatgcga tgaatcatgg ggagcgcaaa cagccggttt
     1321 cttctattcc atcctataca ttttgggata gtatctacgg aaatctgttt gaatttgaca
     1381 cacttatcaa gcatttagaa aaaagaggtc ttctacatga tcagcttgca gttggcgggg
     1441 tttcgatggg gggaatgaca acatgcgcct tgttggctaa acatcctcat atagtagcgg
     1501 gcgtgtgttt gatgggttcc cctgcacctt tggaatatgg aaaagaaatc gtaaggcgcg
     1561 catcggaata taattatgtc gtaccaaaag actattttga tttgatttca tgggttaaga
     1621 gctatgattt gtcattgcag ccggaaaagt tagctggacg tcctctgttc ttttggcatg
     1681 gacaagctga cgaaaaaatc ccttatatac aaacggaacg atttgtagaa aaaaatcggc
     1741 ataagtctta cggaaaagaa attgtttttc gttcctcaaa aacacagcga catatggtac
     1801 aaatacaact aatggaagaa gcttccactt ttttagcaaa aaatttgata aaaaagtata
     1861 aataattgaa acggaaaatc actcatgacg atagaggatt ttccgttttt tattttgggc
     1921 tataataaac tctgttagga aaatttggta taattgatag taagaatgac aacatgttag
     1981 aaaggaagag agcatgccca ctttagcaga tgttgcaaaa cgtgccaatg tttcaaaaat
     2041 gacggtctct cgagtcatca atcatccgga acaggtgact gacgaattga aagagttagt
     2101 tttctcagca atggctgaac tggattaccg tccaaatatt gctgcaaaag cacttgtcac
     2161 gaatcggtcg cagatcatca aattatttat actggaagaa atcgacacaa ctgagcccta
     2221 ttacatgaat ttgctgatgg gaatcgctaa acggatcggt aaagcgcatt attctctcca
     2281 attagttaca aatgatgcct tcgacgtcgg ttcttgtgat ggatacatca tcacaggtgt
     2341 cagaaaaaaa gacttcgatt ggatcgaccg tttggaaaaa ccagttgtct tgtttgggga
     2401 aaattctgaa ggatatgatt atgtagacag cgataatgaa tatggtacag caaaagcaac
     2461 gcaatatgcc ttagaaagag attatcaaac gattatttat atcggtatcg aagtggacga
     2521 gccttttgaa atttcaaggg aaactggtta tttatctgtg atgaaggaag ttgatcttcc
     2581 tagtaaaatc ttgcggtttc caaatcattc gacagaagta gaaagatata tgacagaaaa
     2641 ctgggcagat tttacagata aaacttgttt tgtatgtagt tcagatcggc tagcactagg
     2701 agcagtaaga gggattataa atgctggagg acacttgccc gaagaatttg gtgtgattgg
     2761 atttgatggg gtatttttag accaaatcag ttcacctaaa ttgacaacga tcaagcaaga
     2821 tattatacgt atgggtgaag tatgtggaga aatgttgtta aaaaaaatag aagaaaaagg
     2881 ggcgagtcaa ggttatcgtc gtttttttcc tgaactagtt atcagagaga caacgaatta
     2941 agtcttatta ctatatatat atttatgaaa ttcatcgcta ttttcttaaa ataatcagcg
     3001 cattgctact gaaaacaatg aatctgtgct attattgaga aaagttccat ttttgagaag
     3061 gatggtttga atgaaagaaa tcagacgaaa acatttccgt tttcctgcgt acgatgatga
     3121 gttaggcgta aaattaacca acgacaagaa gcgagtatta tttgacggac aagaagatct
     3181 tttgactgaa cagaatcagt ccccgtttga acagatgaaa gaatttcatt tcacgaacac
     3241 gaacaccgag aaaatccgtc gcaagcgtcg tgcaacaaat ggattgaagt cagctccgcg
     3301 taaggaaaat ttggaaaagc ataaaggaaa attaccggat tataccggcc attaccaatc
     3361 agaaatcaca tcgacaggac gaaaaaaact ttttggtgat tcctctcgtt ctgtcagaca
     3421 aagtgaaaca aggacagagt ccaagcatac tacacgaaac gcatctgttg acacgaaggt
     3481 gcgggagcgt tcttattttg ttcctaaata tgtacccgca tcgattatcc cagaccctag
     3541 agaaagcaaa atttctgagg atcatttaat cgaatcgatg aaaaaaacgg atgactctta
     3601 tctgttgttc gatacagaac cgacgccgta tcaagaaaaa agaaatggtg agccgtctgt
     3661 acgaacattc aatcgaacaa aaacgttaga ggaaacaaag cagtcagaag aaaaaaaatc
     3721 gaaaaaaagc catggtatct tagaacgctc attacaagga ttgatcgatg atcaaggaaa
     3781 ctctttggga gaaaatagct attttaaata agtgatatac aaatttatat tcggctacag
     3841 ttggaaagga aacgctgcgg cgtttcc
//
LOCUS       DAZAFF010000163         3792 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000163 DAZAFF010000000
VERSION     DAZAFF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.173"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(632..3781)
                     /locus_tag="ACG9BB_002853"
     CDS             complement(632..3781)
                     /locus_tag="ACG9BB_002853"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFN2838707.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1056 a          789 c          625 g         1322 t
ORIGIN      
        1 aaattgatct atcaataatt atcaaactga gtcatataga gacaaaaatt tcaaaaatga
       61 cctgttaaat ggtcatcgaa tatcatttaa aatcaatacg acggagtcag acacggagtc
      121 aaaacaagcc cgctgagagt aaaaaatata tacagtccca taggtaaagt gccacaggac
      181 tgtatatttt tttatttgag attacctgaa atgattccat ttacttttta tctcattttt
      241 tcctactagt tagtatcaaa tactctccta ttttctcaat aaactatttt gggttacatg
      301 gtatctgaaa cagctaaaac cttgtcatta cagcaaaaaa tcactttatc tccgtttcat
      361 ttcagataca tttcacttaa gcggattcca aaaaaacaaa agaaaaagga acccctagtt
      421 agcctatgct ttttattggt tcctctcaaa acacggtcat ataacaacgg ggtggggtaa
      481 atgaatgaaa aaggtggagg aaacattatt caatagctgt cttaaatact tcgttataaa
      541 atgtgaacaa ataaaagaca tctatcccta ttttctacac ttccacttta ctaccatctt
      601 tgaatcgtac aattttctta actcttctcg tttacctcaa ttcattttca agtggaatca
      661 gctcatcatc taacatttta cgccatgctc gcttcatttc ttgtgggtat ttaaatggct
      721 tcgcttctgg atggttggct ttatatcctt cgtaatcctt actgttgata attgctttca
      781 tattaggaat tggatcttca cctggcatat attgcagatt agaagcatgt aattctttct
      841 cagaaacaaa ccactcattg gaaaagacat gaatcaaatc atcactcgct tgtgtaaaat
      901 agcgacgttt aatgacaaag atatcttcat tactatctaa agtccccaca tcaaacgcat
      961 ttaaaatttg tcggtaaact tctttgactc cttctgtttt attcaattta gccaaagctt
     1021 tttcaatttc ttctcgtgca tctgaactat tcgccgcata agttcctagt aattgatcaa
     1081 tataagtaga atcaatatca tacaacttcg tggtattttc actataaaat tcgatcgtgg
     1141 aaaaatcttc tccctctttt tctggttctt cctctattaa tgaacccttc acggtctcgt
     1201 acacgccaat ttgttcttcc aaaagttgaa tttgattgtt caaggttgaa gatttcacca
     1261 tgtcatcatt gtaatcgtca taagtcacta acgattcata agcacgattc aattcttgga
     1321 atgctttgac gtactcgata cggatttcaa gtggcgtatg ttcatcaatg gattccatgg
     1381 atgaaacctc ttttaactta ccatatgctt gcttgaatct ttttttggat tcctgataac
     1441 ttggataaat caaacccgat tcttcttttg cttctgaata cagttttgtc gcatcctcga
     1501 cattcttctc cattgtggct ggctttcgga aagtaacaat tagtcctttt gttttttctg
     1561 gatacgtacg gttcgtacgt gaaaaagctt ggatcaatcc tgcatactct agattacgat
     1621 ccacaaatag ggtttgaatc gtaggtgcgt caaaacctgt caatagacga tccactacga
     1681 tcactaaatc aatttgtttc ccaaattgtt tgaattccgc tctttttcgt gccaaacgat
     1741 tattgatgtc tccgttataa cgctcaatgt ctgctaatga ccaggaagta tcataatact
     1801 gattatattc ctcaataatt ttttgcattt cttcttgttg gacacttgaa tctcggctgt
     1861 tttcttctaa tgaataggta atagcgattc gtggaaaatc aggatcctcc atcgttcttc
     1921 cttcacgaat gggctgatta ggaaactctt ttgttaacca gtcaggttct ttcgtcattt
     1981 ctttgatcgc tcgataatac tttttggcca tgtcaatgga actcgtggtt aagatcgcag
     2041 acttcgttgg acggccattt cgaaaatcaa attttgtata ggcattgtct ggacggaaca
     2101 ttttatgaat gactttttgg atatgttcat ctgcttcaaa aacagtaggg tccaaataac
     2161 tttctttttt gacaggctcc atttgatcaa tcatacgatt gatctgttcc ggtgaatact
     2221 ctgcatatgt ttctacttct tttaatttcc gatagatttt attctctaat gaagtcggct
     2281 ccacggtgtt ttcatgctct acttgaaatc ctaaaaccga gccatcttct aatgcattct
     2341 tgatggtata ggtatgcaag acctctccat actgatcatg ggtcgtacgt gccaattgcc
     2401 ctttggcttg tttccgattc tcagggaaaa tcggtgtacc tgtaaagcca aaccaagtgg
     2461 atttcggaaa catttttttg atttcttcca tattttcagc acttaacgct cgatgacact
     2521 catctactac aaacacgata tgttgcccca ttaatttttg gaagcgattg gttccttttt
     2581 tctcttcttg tttttttgca tatttcaacg ccgcatctag cttttgacgg gttgtaataa
     2641 tcacaacatt tgcattggca tctgccaaca gagtttcact taactctttg gcacttcctg
     2701 ttcccacgat caagctattc gctttggcgt tcccggaaga aatcccagta ttaaattccg
     2761 aagcaaattt cgtaaactct gtggtggttt gattatctaa atctttgcgg tccactaaca
     2821 tgatcgtccg atcgatcccc gattttcgtg ctaaaagctt agtagataca aaactagtca
     2881 acgtttttcc tgaaccagtc gcatgccaca catatcctga ttgatgttta tttgcagcaa
     2941 taaacaaggc ttgaatcgca tgtacttgat aaggatgtaa caccatgagg attttattgt
     3001 cttgatcttc actgacaatc gtataatccg caatcaagcg atgagcatcc gggatattta
     3061 gcacttgttt gcaaaactca tataagtttt ctaccttttt attatctttc gttcgccaac
     3121 taaataaaaa cttaggatgc aaatcctttg gtaaggcatt tgcaaaatac cgagtggttt
     3181 gttcattgga aaccacaaac aactgtaagg tcgaaaagat attgttccga aacatacctt
     3241 cttctgcata ttttttgatt tggttaaacg cctgataaaa actatctttt gcggttactt
     3301 gtttcagctc gatttggaca atcggtaatc cattgatcag taaggtgacg tcaaaacgtc
     3361 gatcccgtgc ttctatgtta cttccacgtt ttgcaatttg atggaccact tcataggtag
     3421 agattcctcc accaatgtct tgattagaat aaagaatgag cgagacagaa cccagttgcg
     3481 gatcttcccg ttcaatcgtg atccgagcca ttccattttc accttttaac catttcgcag
     3541 catcaaaggg ggttttcgtt cgtagtaaca attctgtttt gatcgtttca aattctttat
     3601 cggtcaaagg aaaatcattc agttctgctt gattatttga gatgattttt tggcgtaaat
     3661 tgacccaaag atcttcttct gattttagat cgggacgata cgtccactga ttatgccctt
     3721 cccccaatac acggatcaac tgttcttcga cttccgcttc ctcacggtga ctaattttca
     3781 tttatcttcc ct
//
LOCUS       DAZAFF010000164         3718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000164 DAZAFF010000000
VERSION     DAZAFF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.174"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACG9BB_002854"
     CDS             complement(<1..353)
                     /locus_tag="ACG9BB_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838708.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="ACG9BB_002855"
     CDS             complement(613..1161)
                     /locus_tag="ACG9BB_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838709.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="ACG9BB_002856"
     CDS             complement(1162..2019)
                     /locus_tag="ACG9BB_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFN2838710.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="ACG9BB_002857"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="ACG9BB_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="ACG9BB_002858"
     CDS             complement(2581..2910)
                     /locus_tag="ACG9BB_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN2838711.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="ACG9BB_002859"
     CDS             complement(2987..3259)
                     /locus_tag="ACG9BB_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFN2838712.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="ACG9BB_002860"
     CDS             complement(3259..3522)
                     /locus_tag="ACG9BB_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFN2838713.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1091 a          754 c          473 g         1400 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DAZAFF010000165         3713 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000165 DAZAFF010000000
VERSION     DAZAFF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.175"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..152
                     /locus_tag="ACG9BB_002861"
     CDS             69..152
                     /locus_tag="ACG9BB_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFN2838714.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            277..1014
                     /gene="erm(B)"
                     /locus_tag="ACG9BB_002862"
     CDS             277..1014
                     /gene="erm(B)"
                     /locus_tag="ACG9BB_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFN2838715.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1277..2140)
                     /locus_tag="ACG9BB_002863"
     CDS             complement(1277..2140)
                     /locus_tag="ACG9BB_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN2838716.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2142..2414)
                     /locus_tag="ACG9BB_002864"
     CDS             complement(2142..2414)
                     /locus_tag="ACG9BB_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN2838717.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2431..2640)
                     /locus_tag="ACG9BB_002865"
     CDS             complement(2431..2640)
                     /locus_tag="ACG9BB_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFN2838718.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2718..3614)
                     /locus_tag="ACG9BB_002866"
     CDS             complement(2718..3614)
                     /locus_tag="ACG9BB_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2838719.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1140 a          652 c          575 g         1346 t
ORIGIN      
        1 ttttgtataa taggaattga agttaaatta gatgctaaaa atttgtaatt aagaaggagg
       61 gattcgtcat gttggtattc caaatgcgta atgtagataa aacatctacc gttttgaaac
      121 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
      181 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttctgta
      241 tttatttata gatgtaatca cttcaggagt gattacatga acaaaaatat aaaatattct
      301 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
      361 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
      421 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
      481 gaaaaattaa aactgaacac tcgtgtcact ttaattcacc aagatattct acagtttcaa
      541 ttccctaaca aacagaggta taaaattgtt gggaatattc cttaccattt aagcacacaa
      601 attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa
      661 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
      721 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
      781 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
      841 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
      901 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat
      961 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
     1021 gagtcgcttt tgtaaatttg gaaagttaca cgttactaaa gggaatgtag ataaattatt
     1081 aggtataggg cacctctaat aactaccaat taattcacct caaaatgaaa ttagaaaaaa
     1141 acagcgaaat ttttaaatct atttcttatc gatacaaatt cctcgtaggc gctcgggacc
     1201 ccaacatgag gaaatgtttt tataaaaaaa gagtagttac cgttttttgg taactactct
     1261 tttccttttt taaggtttaa atacctggaa gtttaggtgt tttgggtatt ggtggtgtag
     1321 gtagctgcaa gctttccatt ttttgttgaa ttgctttaaa ttcaggggtt tcttggtgtt
     1381 gattttgaac cattttttgc tctattctct ccaaagtcgg ttgaatttct ttccctgata
     1441 ctttacgatt taattctctt tctaaggtct ctttcggact aatggaaggc gtttctaagc
     1501 ttgaatagag tttcactcct tctctattgt aaagccttat atcgctaaat aagcccgttt
     1561 tatgaagggt ttctaaattg gtcggtaagt ttttgacaac aatatcatgc gcttgttttg
     1621 gtgttgccct agctgtcatt ggatcatctg catacatggt ttcatatcgt tcaatcgttc
     1681 ctaagtatga ttcaattttc ggtactgcca tgacgtatat ttttgtttca taacctttag
     1741 attgaagcat tgtggctgtt tgaatgggaa cgtctgttgt tcgtcctgtt ccttcaatca
     1801 ctaaattata gccttgatcg cttaaacggc taataagtgc ttctgtcatg cgattcgaat
     1861 aaggggtaac gtgttttact acgtcttttt catagagttt cactaattcg tcaaaatttg
     1921 ggtgctgttg tttaaaggta tcattatcaa tgacaataac attcccttgt gtttcttcaa
     1981 aaattgctga tcgcaaactg gttttccctg accctggttg cccaccaagt aaaaaagcgg
     2041 ttggcgattc aaccgctagt ctatttttag ttagtcgttc aacattcttt tctaaacgat
     2101 cttcaaattg cttttcggtg aagtcagtaa tattcgccat gttaagccac tttctcttta
     2161 ttcaaaattt gttttgaata ttgattcatt gaccgccaat actcatgaat agcttgtaat
     2221 tcttcaaatg gttgttgaaa taaatcaact tcttgtgcca cttctaattg atttaaaaga
     2281 ttcacttcta gtaaacgagt atttttagta tctagttcat gattcaaaac ataatttaat
     2341 acccgattat aaacacttgc cgataactca ttaatgattt caatttcaaa tgttttttca
     2401 tacgtaactg ccatttcatt ttcactcctt ttaaagtttg tcaggtaaat atttccgtat
     2461 atattcttct aaggcttgat ccataacttc ttttacgttt ccgccattct ttgctgtttc
     2521 gatttttatg atatggtgca agtcggcacg aacacgaacc gtcttatccc ccattatatc
     2581 tttttttgcg ctgattggcg tatcatttcg ttttgctttc tgtgcgccta aatttcccac
     2641 gatcgctcac ttctttctat ttcttcttac tatttatttt atcatcacct atcacaaatc
     2701 acaagtgatt tgtgattcta ttctttttca ttttctaatt gaatgattcg ctcaatcatt
     2761 tcaaaaaata cgttctcata catagacaaa acttttttgt catagccttt gtgttctgta
     2821 atgccatttt tagaccaagt acttacttta ttacttcgct tgataatatt tcggaaaacc
     2881 aaattatcct ctttatgttc tttgtacagt tcttccaggt ttgattttat cgttgcgctg
     2941 tccgtatcaa ctaaataagg aacaaaacca atcatatcta gtccagggtt aaactgttct
     3001 tgcaaatcaa tcaaatagga aatatagttt tgaatgttgt ttgtactttc ttcttctgct
     3061 tgtaaaggga tcataacgta atcactcgcc acgattgcat tatttgtata aacgcttggc
     3121 gttggtacag tatcaataat aataagatca tagtcacttt ttaaaggtgc taaaagagta
     3181 gcaagcaatc tactttcatt ttcaaacgtc catgagcgag ttaattttgg cagtaacatc
     3241 aaatcaaacg tgccagggat caagtctaaa ttatcagtca aatgaataat agaagaagcc
     3301 aagtttccat ttttcaagcc ttcataaaaa ttgacacgtg gcaattctac ctcaaaagtt
     3361 tttgctaagt cttttgtcaa tgttgcctgt aagtccttat cgatcattaa aacttttaaa
     3421 ttcaatttgt ctgtcaagta agcaaacata gtcgataatt tggactttcc aacaccacct
     3481 ttaaagtaat tattcaagat aataattgct tcatttttat tgtttaaaat acgtcttaat
     3541 tcttcaagta tttttagttt ttccttctcc ataactacgc cccattcttt ttttgtatag
     3601 taatattgta tcattaagaa tcacaaatca caagtgattt gtgattaatc acttgtgatt
     3661 tgtgataagt gatttgtgat taatatataa aagccctctt taaagggctt tta
//
LOCUS       DAZAFF010000166         3673 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000166 DAZAFF010000000
VERSION     DAZAFF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.176"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACG9BB_002867"
     CDS             64..972
                     /locus_tag="ACG9BB_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFN2838720.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="ACG9BB_002868"
     CDS             972..1592
                     /locus_tag="ACG9BB_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838721.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1596..2045
                     /locus_tag="ACG9BB_002869"
     CDS             1596..2045
                     /locus_tag="ACG9BB_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838722.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            2045..2539
                     /locus_tag="ACG9BB_002870"
     CDS             2045..2539
                     /locus_tag="ACG9BB_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838723.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            2553..2984
                     /locus_tag="ACG9BB_002871"
     CDS             2553..2984
                     /locus_tag="ACG9BB_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838724.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            2986..3123
                     /locus_tag="ACG9BB_002872"
     CDS             2986..3123
                     /locus_tag="ACG9BB_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFN2838725.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            3160..3453
                     /locus_tag="ACG9BB_002873"
     CDS             3160..3453
                     /locus_tag="ACG9BB_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838726.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            3450..>3673
                     /locus_tag="ACG9BB_002874"
     CDS             3450..>3673
                     /locus_tag="ACG9BB_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFN2838727.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT         1325 a          545 c          811 g          992 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
     1681 cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata ggaaccgttt
     1741 ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct agatttaatt
     1801 ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact acagctgtag
     1861 ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg gatggaattg
     1921 cagctggagc acagaaaaat ccaggtaatg ctacaactac gacagcaggt ttaatgtcag
     1981 caaccgataa agtgaaactt gatggattag cgaatattac atttgagaaa gtggggacgg
     2041 tttaatggct gatattgttc aattaaaaga agacggagtt gctaaatact taaaaacgca
     2101 tgtagaagct attgatggta aagaagcatt ggtgcagaca gatggagacc aagccattgc
     2161 aggacataag aatttttcag gttctgtaac aatcaataat aaacgtgttt tgacgactga
     2221 tgataacaga tatgaagtgg taaatctggt tgtcactaac ggtaatacag ggacagcaaa
     2281 gctttatcgt gaaggaaaaa cagtttctat atattttgtg gctttaaacg gaaaaagtag
     2341 tggcgggaat gattcggtta ttttaactgt tccagaaggc tatcggacac caattagttt
     2401 tgaacaactg gttgggtcaa tagaccgttc tactttgaac agtgctcaac tatctattgg
     2461 cgcagacgga gccattaaat ggagaagaaa ttcaagttat ggatcagctt attcatttgt
     2521 tatcacttat tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc
     2581 cttacggatt cgagcaaatc attttgaaca atcaagaaaa tatccctgaa aactgtacag
     2641 agattaaacc accagttcct aactggagac caagatttga ttttgataaa aaacagtggg
     2701 ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt tgatagcgta gatgaattgg
     2761 ctaatattaa ggcattatac gaaacgctga aggcagaaaa tgatgaattg aaacagctaa
     2821 attctaaagc aatgctaaac aatgtagcaa tcaagcaaga aaatgtctta ttgaaagaaa
     2881 agtcagaaag tttagcgcag ttgaattcaa aaacgatgct tgcttctgta caaaatacca
     2941 aggaaatcga agaaatcaaa aaacaattac aaggtgggaa gtaacatgta ttcatatgaa
     3001 gatatcaaac taatgtatga ctggggctgt ttcactaacg aacaagtaat ggttttcgtt
     3061 ccattatgca ttacagacga agaagcagat aaaatcatta ataaagataa gagcgcatct
     3121 taattgatgc gtttttattt aaggtaaagg agttgtcaca tgattaattt aggggaatgg
     3181 ggaatgatag caggatcaat aaccgctatc gtttctttga ttttattagt aattagaccg
     3241 attgctgcat ctttctcgaa aattactgag actctttcaa aagtaagcca caatttagat
     3301 ttgctgacta aagatttaga atcgagcaaa tcagatcgat tgatgattca tgaagaacta
     3361 aagaaacacg atgaaagatt agatacacat acagaaaaat tggtggaaca cacgcaacaa
     3421 atcaaaactt tgtttaggga gagaagaaaa tgaataataa aacgttcgaa gtactaaaat
     3481 ggttcgcact ggtaattatt cccgcactag ctactttcgt ggggttagtt ggtaaagcgc
     3541 tcaattggca gtacacagat atctgtgttg tcatcattac tggttttggc gcgtttttag
     3601 ggagtgtgtt gggtgtatca aatcgaacct acaaaatgtt ctcggctgaa agcgaagaag
     3661 gaggaaacaa atg
//
LOCUS       DAZAFF010000167         3558 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000167 DAZAFF010000000
VERSION     DAZAFF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.177"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(179..856)
                     /gene="nth"
                     /locus_tag="ACG9BB_002875"
                     /pseudo
     CDS             complement(179..856)
                     /gene="nth"
                     /locus_tag="ACG9BB_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(863..1561)
                     /locus_tag="ACG9BB_002876"
     CDS             complement(863..1561)
                     /locus_tag="ACG9BB_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFN2838728.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1829..3013
                     /locus_tag="ACG9BB_002877"
     CDS             1829..3013
                     /locus_tag="ACG9BB_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFN2838729.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3019..3462)
                     /locus_tag="ACG9BB_002878"
     CDS             complement(3019..3462)
                     /locus_tag="ACG9BB_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFN2838730.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          917 a          716 c          597 g         1328 t
ORIGIN      
        1 ggtattttcc caataaaaat aacaactttt atttttatcg caataaattg taataagtta
       61 ccatcctagc tactgagtat gtacatttac tttttcatgc aaaaaagata aacgaaacag
      121 cttttctcca taataacaag ataattcata catttatttg caattaaaga aaccacgtct
      181 acaggcgtgg tttctttttt agcgcctttt cttttagacg catccgattt ttcccatctt
      241 gacatattga tagcaaagga catacctcgc acttgggatt tcttgccgtg caatgatatc
      301 aaccaaaaaa aatcagtgta tgatgtgtct ttacccataa ttcttgtggt actttccgca
      361 tcagtgtttc ttctacttcc atgaccgtag catctagttt gcagatcctc aggcgttttg
      421 agacacgttc cacgtgggta tctactgcga ttgctggtat cccgaatgca tcccccaata
      481 cgacatttgc tgtctttctg cctacaccag gaagagacat caattcttct cttgacgttg
      541 gcacttgccc atcgaaacgt tcaatcagct gctgtgcaca cgctttgatg ttttttgctt
      601 tatttcgata caaaccaatc gtttttattt taagaatgat ttcatcgatt gaagcttctg
      661 ctaacgcatc gggtgtcgga aaagaggcaa acaaatctgg cgttgcttta ttcactgata
      721 catctgtcgc ctgggcactt aagatcactg caatcaataa ctcaaatgga ttattgtgct
      781 tgagttctcc atgtgcttcg ggaaacattc catacatcgt ttctagagct tccattgttt
      841 tttgtttgct taacatattc cgctactcct ccaaccaatt tttcagtgag actttcggca
      901 attcttcttg ttgagctgac tgttctactt ctctttgaag aagctgctgt tttcttcgtt
      961 tttggtcttc ttccacttgt tgttttgtac gtagattttt tctttcccac gatagcaaaa
     1021 tgcggtcaac gtaattaaaa ctataagcct gatttaatac ggcttcccgc agtgctagct
     1081 tcaaaatttc cggctgataa tggtcttctt ctagccattg tccgatccgc tggaactcga
     1141 tcgaagaaag cgggcgtcca aattcttgtt caaataattg atacactgac tggatttctt
     1201 tttcttgtgt tttttcttcg tatttttttt cttgtgtttt taaatactca cctaatagat
     1261 catagatggg gtaaagatta tatcgatctg cttttttacc gttgacaatc gtcgtttcta
     1321 tcttgataaa tccatttgtt acgagacgat ccagtatttg aaaaatctga tcttgtttga
     1381 ttcccatatc catagcaatc atctgcaaat cagggaaagt atctccttca agctgtgcca
     1441 tatgcaactg tagaacaaat aaaaattctt cagattgtag accgatccta tgataatttt
     1501 taagtaaaag attagatatg actgtttgtc cagcgcttaa gtaattttct aaattcaaca
     1561 tgatttctcc tccgctcaat cacagtatac gtaaaaaaaa catgggaagc aagaacaggt
     1621 ttcttttcta tttacttcac tgatcggtca gatttacaat gtcgtctact tgatttttgt
     1681 ttgttcatgt ttttgatgcg tatcttcact tttcggaata aataaattaa gtagatttcg
     1741 ctgttcctta gaatgttgac aaggaaacaa aattactata cactattttt gtttccaagt
     1801 taacattatt gcataaagga gtttttacat gtacagtttc acaaataaac attctaaagt
     1861 ttttggatac gtctctgtct tattcactgg attaggattt actatcgtca gtcctgtact
     1921 tccgttttta acgctgcctt attctcactc catacatcag caagctttct atatcacttt
     1981 gctcatgtct gtttatgctc tagctgcctt cttatctgcc cccattctcg gtagcttaag
     2041 tgatcatttt gggcgtcatc cgatccttat tatcagttta ttaggatcaa gtatcggata
     2101 tttgcttttt ggtttaggaa attcgatatg gatgttgttc cttggtcgta tcattgaagg
     2161 attgacggca ggagaaatct ccaccttata tgcttacttt gccgacatca cggaacctaa
     2221 tgaaagaacc aaagtttttg gatggatggg tgctttagtc ggtataggaa caacgctggg
     2281 tcctattata gggggtctat tagctgagtt ggggaattct gttcctatat ttattggagc
     2341 actctttact ttcttaaatg ccgtttatgg ctacacattt atgctagaaa gccttccaat
     2401 aaaaaaacgt tcagtagatt taagtttctc ccatgtaaga ccttttcacc aattacaaca
     2461 attattcaag atttcatccg ttattccttt attgacagca ggttttgctg tttggttggc
     2521 agctggctct cttcaatcta tcttttctca attttcgatt gacgcctttc agtggaaggc
     2581 tgggttggtt ggtttaagct tctctttgat tggaatcttg gatactgtct cgcaattgtt
     2641 cattatgcct cgattgctca aaaaattttc agaacagcag atcacaagaa tagggatgtt
     2701 cagtgaaatc ctcgcctatc tattcatcac attatctgga attcttctac tgccgatttt
     2761 gtttttattt gggattatct gttatggttt cggtgattct atttttactc ctgtattcaa
     2821 tggccaacta tccaattctg tctcagataa ccagcaaggt cttgtaatgg gcggtaccca
     2881 aagtatacaa tctctatcgc gcgtcatcgg cccgctaatc gcaggacagc tttatgctgc
     2941 agcaccattt cttccgacgg tattcggatt tttgcttatg atttttgcct attatcacta
     3001 caataaaaaa tagtagcttc atttgctact tcgtataaat tctctgtatc gaccgataaa
     3061 acgaaatact gtgtcaaatt cttcttttga catattttca aatacagtag catctcgctg
     3121 tacccatttt tgatggagtt gctcgtgtct attccttatt ttttgtccga ttggcgtcaa
     3181 ttgaaaatag atttcttttt tattctcttc gctttgataa gtgtttatga tccctctagc
     3241 tatcagtttt tttgttagct tactgatggc tcctctagtc atgtaactag cattcgctaa
     3301 ttttgtcacg ttaggatcag ggattttccc gatatgttca atcacttcta gttcatttag
     3361 agtaaatcct gcaaattctt tttccatttc tggtttatta cgatgaataa tctcattagc
     3421 taaatccaga tattcttcta ttagcagttg tcttttatcc atttctacac cttctttttc
     3481 tttcattata ttgttttttt gtttcctatt caacaaagat aaaaatattt tgtaggaaaa
     3541 cccaatctga aaccaaga
//
LOCUS       DAZAFF010000168         3546 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000168 DAZAFF010000000
VERSION     DAZAFF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.178"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..189
                     /locus_tag="ACG9BB_002879"
     CDS             <1..189
                     /locus_tag="ACG9BB_002879"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838731.1"
                     /translation="TSDKRCSKASFSVLSQIYAKMWKAIFTKFFLILETKRLFHNLLI
                     NGVQKLASAVISPTKRKI"
     gene            complement(259..1212)
                     /gene="menA"
                     /locus_tag="ACG9BB_002880"
     CDS             complement(259..1212)
                     /gene="menA"
                     /locus_tag="ACG9BB_002880"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFN2838732.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1225..2328)
                     /locus_tag="ACG9BB_002881"
     CDS             complement(1225..2328)
                     /locus_tag="ACG9BB_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFN2838733.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2418..3353)
                     /gene="pplA"
                     /locus_tag="ACG9BB_002882"
     CDS             complement(2418..3353)
                     /gene="pplA"
                     /locus_tag="ACG9BB_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFN2838734.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
BASE COUNT          997 a          733 c          626 g         1190 t
ORIGIN      
        1 acctctgata aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa
       61 atgtggaaag ccattttcac aaaatttttc ttaatactcg aaactaagcg actttttcac
      121 aacctcctga taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga
      181 aaaatataag gagctatttt tatttggctg ttcttattac ttgatgcagg gcagtttttt
      241 cacaacctct tcatgctgtt aaatggcttg cataatgatt gttagtaata gccccaatac
      301 ataactggca ttgaataaga ccatattttt gatggaatag ctgaaactga taggctgggg
      361 taacgtttgc ttgaattgat ccaaatgctt cttcacaatt ggcaaggtca agaaggtgac
      421 caagatcggc cattcataga taccgctcaa caaccccatt aaaatcacga aatagcaagc
      481 atacatcaat agctggaata agactgttcc cgcaggacgt ccgatataaa aaactagtgt
      541 atagcgatga ttttcaatat cacggtcaag atcccgcaaa ttattagcca acatgatatt
      601 ggcaatcgta aacaccaagg gtaaggaagc taatacgatt gctgctatag cccaaagatt
      661 tcctaccaaa gcaaattgtc cagatgaaaa gttgactgtc agatcataga catctgaacc
      721 gaatgtattg acataaacga ccatggcaaa aattccaagg cccatagtca ccccactgaa
      781 aatttcccct aaaggcattc gagataaagg aatcggtccg aatgtataga atactccaat
      841 aaaacaacag ataaatccca tcaaaagtaa aagccaaccc gtattgatcg ttagaataac
      901 gccgataaca aaaacaaatg caatcatagc taaaatcatg tttcgaatca atacaggaga
      961 tagatttctg cggccaatga cattttcttc atacttatat acttcagaat gagccttttc
     1021 ataatccatg taattattga tagcggtagt cgctaaatca aaaactagca ttccaataaa
     1081 aaataaaaaa gtgtttagcc aatgtatctc atgaaaaaaa gcgatagaga ataatacccc
     1141 taccgcaaaa ggaaatacac tagccacctt tgttcgtatt tcaactactt ctaaaaagat
     1201 ttttagtgtc atggttcgta ctccttattt tagttcagaa cggtcaccca tcgtgtaatg
     1261 ggagtcctcc gataatttga aggtgtcttt gattggatcg gtcacatata ctttatcgtc
     1321 ttttgtcacg aagatcgctt ccgtcccgtt attcaattcg ctttcgacgt attccagtcc
     1381 ttttttcaca cccattgaaa agactgctgt agacaacccg tcaccatcaa ttgatttatc
     1441 tgttatgatc gtgacacttg cgatatcatt gtcgaacgga taacccgttt ttggatcaaa
     1501 caaatgatgg tattttttgc catctacttc taaataacgc tcgtaaattc cagaggtaac
     1561 taatgttttg ttccgttctt taatagaacc taaaacagat ccgcgagcca tgttcggatc
     1621 ttggatgccg actgtccagt cttggttttc gcctcttggg ctgtggccta agacatagac
     1681 attaccgccc aagtcaacga tggcagtggt cactccttgt tttttcaaga ctttcactac
     1741 ttcgtccgta atgtaacctt tggcgattgc acctaaatcg atgatcattc ctttttcttt
     1801 tagataaaca gttttttctt tgtcatttag ttcgatctta tgataatcca ctaatttcaa
     1861 tgcctgatcg atttcttctt gggaaggttt tctggcgtca tcaaaaccga ttcgccataa
     1921 ttgtgtgatt gcaccaatcg ccatatcgaa tccgccttga gaatcttggc tatattcgta
     1981 ggcccgcttg actaacgtgt agacgtcatc agaaactttt actggtttga tcccagcttg
     2041 ctcattcact tcatcgatct cagaaccttt ttgattgatc gtgattttat caccaagttc
     2101 ttttactcga tcgaaggcag gtttcaatgc tgattctttg ccttcatcat acacacggat
     2161 acgaacgtaa gtccctaaca agaactgttc atcagaatat ggatctttca atatcttggt
     2221 agctgctgaa ctttcagtgg ttgttttatt tgctgagttg ctgcagccag ttagcaccat
     2281 aaaagagatg cccagtgtga ttaaggaaaa tagtagtttt ttccgcatct ttttcctctc
     2341 ttcattttga aaggtgagac gactgcctaa tctggctatc gtcccaccct ccaactttta
     2401 ttagtatgat tatgtgatta tttttctgtt acgatattat ctacttcgat tgtttgagtg
     2461 tcgccttttt cagctgcatt cactaattgt tgtgcgtaca tgatgaatga atgtgagctg
     2521 tgtgtagcac ctgtaacaac ttctacatta gcaggtgagc catcttcttc gcccattgct
     2581 ttgacgaatt cgtcgttcag agtagggatg taagtttttg gttctgtacc gctttttgct
     2641 ttcatatttt cattatattc tgtatcatct tgttttgatt taccgtcttt gtcgatataa
     2701 tcaaagtttg attcagttac tttgccgcca gcaactgtca tttcgaattg cacacggtag
     2761 ccgttagagt agttcttttc ttcaagcttg taagtgccgt ctttcaaatc agcaccattg
     2821 tcgatttcga ttgtatctgt gttaccagct tgagcagctt ggatcaattg ttgtgcgtag
     2881 ttttggaagc tttcagatga gtgagtagct ccagttacga cttccacacc gcttgcgctt
     2941 tgtgctttta agaattggtc attcaactca ggaatgtatt tttctgggtt tgtgccagat
     3001 tttgcttcca tgtttttgtt gtattctgca tcttttgttt ttgattcgcc gttttcattg
     3061 acgttatcat attttgattc agtgattttg ccgtctttta caaccatttc aaagactgca
     3121 cggtaaccat ttgaataatt tttttcttct aatttatatg tgccgtcttg caaatctccg
     3181 ccagctacaa cttttgcaga tgattctgtt gtgctttttg cagtcgaaga ttgtgctgta
     3241 gatgattcag aagaagagtt tgagcttccg ttgtcatctg cgccgcaagc gcctaacact
     3301 agagctgaaa atgctaatac ggcaaatcca gttacaaaac gttttttctt cattttaata
     3361 ccccaccatt ccatttctag tccacaattt ttctttgtga ttcattatac attctaatct
     3421 ttttagccta taagtcaact atttttgata aataacccat gtaatatttc aaacgttttc
     3481 tttcagcaaa tttcgtgaaa aaacgcttta ttttaaaatt tgaaaaaaat ccaaaaggag
     3541 ttagac
//
LOCUS       DAZAFF010000169         3519 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000169 DAZAFF010000000
VERSION     DAZAFF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.179"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..1363
                     /locus_tag="ACG9BB_002883"
     CDS             32..1363
                     /locus_tag="ACG9BB_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFN2838735.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1329..1679
                     /locus_tag="ACG9BB_002884"
     CDS             1329..1679
                     /locus_tag="ACG9BB_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838736.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1771..2190)
                     /locus_tag="ACG9BB_002885"
     CDS             complement(1771..2190)
                     /locus_tag="ACG9BB_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFN2838737.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2191..2535)
                     /locus_tag="ACG9BB_002886"
     CDS             complement(2191..2535)
                     /locus_tag="ACG9BB_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFN2838738.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2885..3481
                     /locus_tag="ACG9BB_002887"
     CDS             2885..3481
                     /locus_tag="ACG9BB_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFN2838739.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          924 a          640 c          642 g         1313 t
ORIGIN      
        1 tagttaaaga aaatgaaaaa gaaggtgtgt aatggaaaac gaaactgacg ttatttatat
       61 tcatcctcaa aaacgaatcg tttcacagaa aagaaagtat ttttatcttg gttttacagg
      121 tgtttttttt ctttttattg gcttgctttc taatactcct actgataatt ggagtggatt
      181 gctaactatt cttacctcgc caagcaatct tttgacagat tattttgctt taggtgtttt
      241 cggaagcgca ttcataaatg tagggatttt gaccttactt tctgtgcttt tagcttatcg
      301 gcacaaggtg attttaaacg gtcccttatt tgcttcgatt ctaacagtta ccggtttctc
      361 tttttttggc aaaaaccttt ataattctat ctcaatcatt ttaggtgtgt atctatacgc
      421 tgtatttgtc aataaacctt tttctcagta catcatgatc ggactgtttg gtagcgtatt
      481 atcaccggtc gttagttata tcacttttgg tatgcgtttt cctttactga taggtatttt
      541 attggggaat ttagctggca tagcaattgg tatacttctt ccgcccttag ctgctcaaac
      601 tttagttttc catcgtggat tcacacttta taatattgga ttcacctctg ggttgattgc
      661 gatgactttt actgctgttt tgaggttatt cagctattca atcgtcgaaa atacattggt
      721 tttcaatgaa tatcactttc ctctcatttg gattattttt ggcttttttt cgctgacagt
      781 cggaattggt ttctattata attcctttcg gctttcgggg attcgagaaa ttttcgattc
      841 atctggaaaa ttgacaactg atttcattgc taattctggt attggtgcta ctttgatcaa
      901 catgggctta gttggtctga tgctgtcttc ttatgttttg ctggttggcg gtcagttaaa
      961 tggacctgtc atcggtgcta ttttatctgc ggtgggtttc agtgcttttg gttgtcattt
     1021 aaaaaacagt tttccgatac tagtcggaat atttattgct tctttgtttg gaacttttca
     1081 tgagatcacc agtactggca tgcttgttgc agctgtgttt gggacaggtc ttgcgcccat
     1141 aagcggtttt tacggcagtt tttacggtgt aatagctggt gtgctacata ttgctcttgt
     1201 ccataacgtc agcactttgc acggaggact aaatctatat aacagtggtt tttcaactgg
     1261 tttcgttgca ggtatccttg taccgatact agataacttt accgctgtca gaaaggaaaa
     1321 aaagacactt ggaaaaagaa ttatcaaaaa gaatcatcga tgagcttctt acttatttat
     1381 attcccatga aataactgaa atacgtattg gagtagaatt tgctgaggaa gggcttttca
     1441 tagagataca gggtacggca gaaactcagc cagaagattt agatgtactt gctgatttgc
     1501 tgaacaatcc tcgggaccct agtatggaaa tctactatca agaactatta ggtaatcccc
     1561 gacatgatcg ggaagattat catctgttag gtagtttgat tgatgacgtg gaaatcatgt
     1621 atgcccagcc gattatcaat attaagatct ttcgacgtgc ttctcattcc tattattaac
     1681 cctattttct atttataaaa gactgaatca aaaaagcagc tcctgaaagt tcgattaaaa
     1741 tctactttca ggagctgttc aagcttgaca ttattgattt tccaatcctt catacacttc
     1801 atctgttaca ggttctaaaa actctgaatt accagcagtg atcgccaaat gttcaaacca
     1861 actgtctttt gtcgcaccat gccaatgttt aatccctttg tgcgttacga cgacatctcc
     1921 agccattaat cgtcttgctg gctttccttc ttcctgatac cagccttctc ctccggtcac
     1981 aagtaagatt tgatagccgt ctgtatggac atgccagtga ttgcgacaac ctggttcaaa
     2041 agttacatta cctacaccaa cagaaacttc gggatcattc actaacatat tcagatagct
     2101 ttgcccggta aatacttgag cgtaagcttc gtttttttca cctgctggaa aaatcacgcc
     2161 attgtgtatt tcttcgtgtt ttgtcatcat ttacgcctct ttcttatctt cgctattttc
     2221 ttctaaatag atttcttttg ctaaactaaa agctgaccaa gcttttggcc attctacata
     2281 aaaagctaaa tgggtaatga tttctgctat ttcttcacta gtaatgccat tttgcttgcc
     2341 aatcttcaga tgagctggca attgttcaaa atttcctcct gaaattaatg cagatatggt
     2401 gatgatacta cgctgatgag cagataattc ttctctggac catacttgtc caaataaaac
     2461 atcatcattt aattctgcaa attttggtgc aaaatctcct aattgatctc gtcctgcggt
     2521 ttgtttttct gccatttctc ttattctccc ttcacgactg ttaggtgatt caactatggt
     2581 tctaaggcaa ctttattttc tattgtttct tcgcatttca ttttaaatgt agtctttcag
     2641 cctgtccaat gcttagttta ttcagtcgat atgcttttta agcatagttg ctaacgcttt
     2701 ttttatttta aaaaaaacaa gttattaaat aagtctttag ttggatgttt ttttgagatc
     2761 aatcttttat acttatgatg aacttacaaa attgtttgtt ttctgtcata tcaaaaaatg
     2821 gcgcagttag ttgcttttct agtaaagtat agttaacaaa gaacctctaa ggagttgatg
     2881 aataatgaat attgctgcct ctcgaaaact gataaatttc atatccatta tcggattagg
     2941 cctatcaatc gcgctgacga tttattttat taatttaggt gttttcaaag atttgaatgc
     3001 gttaagagga ttagtaggtg attccatcat tttaggaccg attatttttg tcttcatcca
     3061 aatcctgcaa gtagttattc caatcattcc tggagggatc agtactgcag ctggtgtcct
     3121 gatatttggc ccttatgctg gatttatcta taactatatt ggaatctgca ttggttcgat
     3181 tatcattttt ctgctagggc gtcgttatgg aaagccattc atactttcca tgattagcga
     3241 taagacatat aacaaatata ttggctggct ggataatcaa aaccgttttg agaagctgtt
     3301 tgctctagct atttttcttc cagttgctcc tgacgatgcg ttatgtttga tggctggatt
     3361 gacaaacatg tcagtgaaac gatatacact aatcattttg attgctaaac cactgtcgat
     3421 tttcttatat agtatggcct tgatttatgg aggtcaatat ctaagtggtc ttcttggata
     3481 atatttattt tgtaaaatta gtcattgatt gacaatgtt
//
LOCUS       DAZAFF010000170         3450 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000170 DAZAFF010000000
VERSION     DAZAFF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.180"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG9BB_002888"
     CDS             complement(<1..2232)
                     /locus_tag="ACG9BB_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFN2838740.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACG9BB_002889"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACG9BB_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          965 a          762 c          548 g         1175 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat
//
LOCUS       DAZAFF010000171         3402 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000171 DAZAFF010000000
VERSION     DAZAFF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.181"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..750
                     /locus_tag="ACG9BB_002890"
     CDS             142..750
                     /locus_tag="ACG9BB_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFN2838741.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            750..1091
                     /locus_tag="ACG9BB_002891"
     CDS             750..1091
                     /locus_tag="ACG9BB_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838742.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            1333..3402
                     /locus_tag="ACG9BB_002892"
                     /pseudo
     CDS             1333..3402
                     /locus_tag="ACG9BB_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT         1176 a          446 c          716 g         1064 t
ORIGIN      
        1 tgtgggactt tttttaatac gattacaatc gatgagcaaa tattatttcc aaaagcctat
       61 ttacctgatg aagcagatgg tgttcaacat attcgtttta cattagaatt tccacaatat
      121 attgaatgga gtgaaagata aatgggagaa aaaagaagta aaaccggaat tatttctgtc
      181 gaaaaaccta cttggtttcc tttggaagat gagacaggga attttccggt atatggtact
      241 gcgttgccaa tgggaacagc agtaagtatc aaaccaacag ctaattatga aacaacacaa
      301 gattatgggg attcagtggt tcaagatcaa tttacagctt ttggtggtgc agaagttgaa
      361 ctggaagcaa atggatatac tcataaagtt ttatctacaa ttactggagg gaaaattgtt
      421 aaaggtggtg ccttgcgttc tggagaagat attgcgcaag atggtgcgtt tgcatataga
      481 cgtaaaaaat cgaatgggaa ataccgttat acggtctttt ataaaggaca gtttgctcta
      541 gattcagatg aaacatcaac gattgaagga agtaaagtaa gttttactca tccaacttgg
      601 aagggttcat ttgtagatgt accaggactt ggatatatgt attcagttga tgaagatgat
      661 gaaggcgtgg ataaagcaat gatcggaaat tggtttacaa aagtagcaat tccaattgaa
      721 gaagcagaac tttcaggagg tacagaataa tgtcaaaata tcaaacaacg attaaattaa
      781 cgaaaaaaga tgaagaaggt aaatatgagc aagtacaatt taaatctgct gaatttctac
      841 caggcccagt cgtagaagaa gcagcagaag taatggaggg aatgcaaaat gctgtagatg
      901 gaaaatcagt atcagaagca ttaagtcgag cttattcatt tattgcagat attttgtttg
      961 aaggacagtt tactggtgaa gattatcgtc aagggatcga tgctcgagaa attgcttcgt
     1021 tgacaggtaa gttattaaaa tctgttactg caggctttga tgaaacttat acagaaacga
     1081 aaaaaaagta agcgaagctc tcaaatatcc tttttttaaa cattcgatta cttatcgaga
     1141 attagattta aagacgcaat tactcgaagc aggttggacg ttaccagaaa ttgaacgtac
     1201 tgactttgac gagttaatgc gtctttttgc ttttcgagat gcagtaaaag agcatgagga
     1261 tgttgagtac tacgataact tcacccaatt ttaggaggtg atactttgaa taacgatgac
     1321 ctaattctga agatgatact agatgaatct ggttttactg ccggtatgaa taatgctgtc
     1381 aaaaagttga actcttttga tgaaacaatc gaaagaaaaa gtagaaatag tggcagttca
     1441 ttaggtagta tttggaaaat atttgctgga agttttctag ctagcggagt aactagaatc
     1501 gtaggagctg gctttgatct aatcaagggt tccataagtg gagcggttga tcgagtagat
     1561 acgatgaata acgctctacg aaatttccaa aatatgggat ttagtaattc agaaattatg
     1621 aagaatatcg gaaagaatgg gcttttatct caaggtattc aaggacttcc tactgctttg
     1681 aatgatgcga taagtcatgt tcaacttctt gcttcttcta caggtgattt aactcgttca
     1741 actcaaatat ttaaagcttt aaatgatgga attcttggtt tcggtggttc aactgatcaa
     1801 gttaatgaag ctgttattca attatctcaa agtttctcaa acggaaaagt agacgcacaa
     1861 acttggaact caatgatcaa cgctcaactt ggacctactc tttctgctat tgctaaaaag
     1921 atgggaatta caatgggaga tctgaaagaa ggtttatctc aaggtaagat ttctgttgaa
     1981 gaattccaaa atcaattaat agaaatggat accaaaggtg gcggaggact taaatcatta
     2041 agtcaaatcg ctaaagattc tactaaggga attaaaacct ctatacaaaa tgcaaaaaca
     2101 gctgttacac gtggtgttgg tgaggtaata gaaggattaa ataaagcatt agtagattca
     2161 gatttaggtg gatttaaggg aattattgat aaagtgggca gttcaatgga atcgttctta
     2221 aaagtaattg ctgcaaatat tcctacagca gtatcatttt taagtaattt atttaacgaa
     2281 attcaaaaat ttggttctgc tttgaaattc atgatgccct ttcttgttcc tgcagctacc
     2341 gcttttggag cgttcatgtt tcaacttaaa ggtataccag caataataaa aagcttcaat
     2401 aattttaaga atgccataat tggcgttgga aattcattaa agattatggg ggcaatagct
     2461 gctgctaatt catttgtttt aattattggt gcagtcgttg gtgcaatagc tgttttcggt
     2521 tactttatgg caaccaatga agagcttaga aacaaggtta tatctatttg gaatgatgtg
     2581 aaagattccg taattggtgt attaaaaaat ataaaggact ggggaattga tacttggaat
     2641 tctgctaaat aaatggcatc aaatgcagtt gagggtgtca aagatgcttg gtcaggaaca
     2701 aaggaatggt tttctaacac ctggaaaggt attaaggatg gggccacagg tttatttgat
     2761 aaaacagtag agacttcaag aaatgcagtc gatagtgtaa aaaatgcatg gtctaatatg
     2821 aagaaatggt tttctgatac ttggcaaagt ataaaagatt cagccagaga aaaatggaat
     2881 ggaatcaaag gttctattat gtcagttgct ggtccattga ttacaggtat aaagaatgca
     2941 tttttacatg ttacttttta tctagagacc ttatggaata atttagttga gattggtaaa
     3001 aacgtctttg agattttaaa aaatgttata cttgcgccgg ttttgtttat tacctccatg
     3061 atttctggtg gatgggaaga aacaaaaaat aatatgattg ctgtttggaa taatattaaa
     3121 gaaagtgcca taaatatttg ggaatctata aagaatatat ttgtaagtta ttttacaaat
     3181 atttattttg ctgcactcaa tatttggaca gggttcaagc ttacattgat aaatatttgg
     3241 aatgaagtgg taaatcaagc taaatcgatt tggttaaaca taaagtattt ttttattaat
     3301 ctttggattg atataaaata ttttgcaatt cagaaatgga ttgaattgaa gttttctatt
     3361 attcaaactt ggatcgattt aaaatataat gccattacta ct
//
LOCUS       DAZAFF010000172         3207 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000172 DAZAFF010000000
VERSION     DAZAFF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.182"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..643
                     /locus_tag="ACG9BB_002893"
     CDS             <1..643
                     /locus_tag="ACG9BB_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFN2838743.1"
                     /translation="KNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRV
                     KEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSP
                     SRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQN
                     ISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            633..1373
                     /locus_tag="ACG9BB_002894"
     CDS             633..1373
                     /locus_tag="ACG9BB_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFN2838744.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1370..>3207
                     /locus_tag="ACG9BB_002895"
     CDS             1370..>3207
                     /locus_tag="ACG9BB_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFN2838745.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1205 a          389 c          621 g          992 t
ORIGIN      
        1 aaaaaatacc atgattaata catggaataa cattaaggac tctttctgga atattgttac
       61 tggaattgtt aattctgctg aaaatgcatg gacaaatcta aaaaatggtg tttcaaatgc
      121 tattaatcgg gtaaaagaaa tctttaattc gttaagggaa gtcaatttat ttgaaattgg
      181 caagaacatc attgatggac ttataaatgg tgtaaaagaa aagtggaatg ctttgaaaga
      241 tactatcaaa ggtattgcag gtaatatcaa aaattggatt aaaggagctt tagatattca
      301 ttctccatca agatggatgc gtgatatggt tggtaaaaat attgttcaag gtattatcgt
      361 tggtattgat aaagagcaaa gtaagttaga tcaaaccatg acaaatttag taaaaacacc
      421 gtctgttcaa cctgtagtaa atggaataag tcacctacca attacacaaa caaagcaaaa
      481 tatatctgct agtgaaacaa aagaaattca tttacattta aatgtttatg gagatttacc
      541 tgattcaatg gttcgaaaga ttactacaaa aattaaaaca gaattgacaa gacaaatgaa
      601 acgagatgct ggtgcagtag gaggaacatt atatgcaact taagagagga caatttttta
      661 ttaatcaaca ttattcttct gaatttaatg tgtatattca aaacaggcct gcttctgttt
      721 cagctagtcg agtaattgaa ttgagagagc gagaaggaaa tgattctatc attattgata
      781 aaggctatta caaaaatgtg actagaaaga ttgagtgtta ctataaagca ccttcaattg
      841 atatggtgca ggagtgggaa gatcgaatca ctgaatggtt agatatgtcg tcttatagtg
      901 attttattct ttactatgat gaacaatata tttatcaagc tgtagtaata gaaggacctg
      961 aatttaaagg aacaagaaaa acaggaaata ttgtcccttt tgaatttaca gtgagtattc
     1021 gaccatttaa ggaaaattac aatggtagat ttactattcg acaaacagaa acttttgaaa
     1081 tttataaccc agagaagtat tcttcaaaac cgcttattaa attgagtggt tctggagatg
     1141 cttcttttta tattaataag gataaatatg atttgaaatc gttggacaga gaattattta
     1201 tagattctaa attagaagag gcttatcgaa aattagatgg taatttagaa catcaggatc
     1261 aggtcacctt atttttagat tttccatttt tatatcctgg aaaaaatgaa attaaatgga
     1321 cgaaaaatat tcattcattt gaaataatgc ctaggtggtg gagaaaagta tgaaaccaag
     1381 aatatatagt cctactgaaa cagatttctc aacgaatggc ttaggaattt taaaggattg
     1441 tacaagatgt gaaatatatg aagtagctaa tggaaaatat gaattagagt tggattatcc
     1501 tttaggaact agatttgatg aatattttga aaatgactat caaataaaag caaagccaaa
     1561 cgatcaagaa gagtatcata tcttttttat tgatgataaa gatatagata cttttttaaa
     1621 tacagtaact atttatgctc agagtcgtac aaatcgactt ggaagacggg tagtcactct
     1681 tgtggaagtg gactctaaaa ctggtcaaga agcgatatca attattgaaa ctaaaatgga
     1741 taaaaaatct gacatacgac tttattctga tattacggct gtttctagca caatctttga
     1801 agcgagaaat gttttaaatt gtattgctgg tgaacaagga tcattgcttc aatattgggg
     1861 cggagaaatt aaacgtgaac catttaagct ttctttgtta aagcgaagag gtcgcaataa
     1921 tgttggaacg attcggtatg gaaaagatat gtctggtttg aaggtcaaat tagattggac
     1981 aggtataaaa acaagaatta ttccatatgc tgatcctcaa agtgaagtag gtacgactaa
     2041 tcgaatttat ggttcgccag tagatagtgc ctatattaat aactatcctg atgtctatac
     2101 agaacatgtt cagtttacag aagaacaagg agtaaaagac attaagagct taaataaaat
     2161 agctaaaaat tactttaaaa cgatcaatcc tggctgtgat aagcctaaag tttctattac
     2221 ggttgaattt gataagttga ctgatagtga agaagcgaaa gaatttgcga agattagaaa
     2281 ttatggtctg tttgatacat ttaaaatata tcataaaaaa tataatattt atcttgaatc
     2341 aaaggttagt ggactacaat acgattcttt atctgaaaaa actttgaaat tagaagcagg
     2401 agacattcaa gttgcttttt atcagcaaca agctgtaact attcaggata aattaaaaga
     2461 ttatgcaacg aataattata tgagtgattt taatgattat gtttcttcaa tgattacagg
     2521 tcaaggcagt gcaggaggat atgtagtttt atggccaaaa gaaaaacctt ccaacatctt
     2581 tattatggat aatccagatt tagaaaaggc aagagaagta ctaagaatga ataaaaatgg
     2641 gattgctttt tctaagaaag gatggcgggg gccttttaat tcagcttgga cattagatag
     2701 tatatttaat gccaatttta tacaaactgg attaatcaaa gcagatatct ttcaaaattc
     2761 ttttaacaag actggagatg tattgaaatt agttaatgga ctactccaaa tttggaacaa
     2821 caaaaagaaa ataatggagt taactaaaaa aggaatggaa ttttggaatt ctaagggttc
     2881 aattggaacg attggaacaa ctgattctgt tggtaatcct tttcctgggg cttctactcc
     2941 aacccctatt gaagataatt ctttagttat tcgtacaaat ggagacggca aatatatttt
     3001 gatttctcct aaagtaggaa aaggattagt tttattagga aatggtaaag caatttattt
     3061 tggagactta gatgtacaag gaaaacttac agttaaagga aaagaaataa ttcctggaca
     3121 aggtggtggt ccatcgggag gtggagaatc tactggtggg tatcctagtg aattaaaaac
     3181 agatgcagaa aagagagctt ggagaat
//
LOCUS       DAZAFF010000173         3162 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000173 DAZAFF010000000
VERSION     DAZAFF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.183"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..2555)
                     /locus_tag="ACG9BB_002896"
     CDS             complement(63..2555)
                     /locus_tag="ACG9BB_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFN2838746.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="ACG9BB_002897"
     CDS             complement(2562..3029)
                     /locus_tag="ACG9BB_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFN2838747.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          838 a          697 c          518 g         1109 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DAZAFF010000174         3081 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000174 DAZAFF010000000
VERSION     DAZAFF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.184"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..806
                     /gene="fabI"
                     /locus_tag="ACG9BB_002898"
     CDS             51..806
                     /gene="fabI"
                     /locus_tag="ACG9BB_002898"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFN2838748.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(916..1968)
                     /locus_tag="ACG9BB_002899"
     CDS             complement(916..1968)
                     /locus_tag="ACG9BB_002899"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFN2838749.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2094..3014
                     /locus_tag="ACG9BB_002900"
     CDS             2094..3014
                     /locus_tag="ACG9BB_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFN2838750.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          896 a          622 c          589 g          974 t
ORIGIN      
        1 ctattttttt acgactatca aatttttatc agaaacaaag gagagtcatt atgtcatttc
       61 tagaaggaaa aaaaatcgtc attatgggcg tcgctaacaa aaaaagcatt gcttggggtt
      121 gtgctgaagc aatgttgaaa caaggggcag aaattatcta tacttaccaa aatgaacgaa
      181 tgaaaaaatc attagtcaaa ttggtcggcg aagaagcatt cactgtagaa tgtgatgttt
      241 ctagcgacga aagcatcgaa aaagcaatga atgccatcgg tgaatatgct ggggagatcc
      301 acggccttgt ccatgcagtt gcttacgcca acaaagaaga attgtccgga aatgtgtctg
      361 atatttccag agacggctat caattagctc aagatatcag cagctactct ttaattgctg
      421 tatcaaaata cgcacaaccg tttcttgcac caaacagcgg gatcgttagc atgacttatc
      481 taggttcaga acgcgcaatc ccgaattata acatgatggg tattgcgaaa gcttctcttg
      541 aagctgctat ccgttatttg gcagctgagt tctcgcctaa aggaattcgg gtcaatggaa
      601 tctcagctgg ggcaatcaaa acattagctg tcacaggtgt taaagactat caaaaactga
      661 tccagttatc tgaatctcgt acgcctgatc agcaaggtgt gacgatcgaa gaagtcggta
      721 atgcttgtgc tttcttaatc agcccacttt ccagcggaat catcggtgat attatctacg
      781 ttgacaaagg tgtccatctt tcataaactt ttgttgcatt ctttaaagta aaacatataa
      841 aaatcaggaa aaaacttttt aaagtctctt cctgattttt tctttttacg tttttttcaa
      901 ctcattactt tgaaattatt ccacgacttc aatccagcct tctggtgctt ctatgtctcc
      961 aaattgaata cctgtcaatt catcatacaa ccgttttgtt actggtccta cttccgtttc
     1021 actataaaat acatgaagat tgttattgtg ataaatccca ccgattggag aaataacagc
     1081 cgctgtacca catgcaccag cttctgcaaa ttgatctaga tcatcaatga aaatatctcc
     1141 ttcaattgct tgcagtccta gtcgttcttc agctaactgc aacaaggaat atttcgtgat
     1201 acttggtaag atagagggcg attgcggagt gatgaatttt ccgtcttttg taattccaaa
     1261 gaaatttgct gcacctactt cttcgatttt cgtatgtgtg gctggatcaa gataaacaca
     1321 atctgagtaa tccagttctt ttgcttctgt tcctggaagt aaactcgctg cgtaatttcc
     1381 gccgactttt gctgcaccag tccccttagg agcagcacga tcgtaatcag atacaataaa
     1441 attcgttggt tttagtccac ctttgaaata cgctcctact ggcgtacaga agacgacaaa
     1501 taaatattct ggagctggat ggacaccaat gttttcaccg accccaatca gtaaagggcg
     1561 caagtataat gtagcgcctg ttccataggg cggcacaaaa tcttcatttg cttttaccac
     1621 ttgcttgaca gcatcaacga acatcttttc agggaccatt ggcatcagca atcgtctagc
     1681 actttgattc atccgtcgac tattttgttc aggtcgaaag agattgacct tgccgtcttt
     1741 tcgacgataa gcttttagac cttcaaaaca ttgctgacca taatgaagcg ctggagagcc
     1801 ttcatgaatc gtcaggtaat tatcttctgt caattctcct tcttcccatt gtccgtcttt
     1861 ccatttggca ataaaacgcc aaggagtttt gatataagaa aaacctagat tgttccaatc
     1921 gatttgcata tacttaccac cttcgttatt ttctccacat tttatcatta tccatattat
     1981 ttgtcatttt attttcagct tttttccttc attatagtta tttttgctat ttcctttttt
     2041 gaaaacttct tggtacaata cagaaagaat caaattttag aaaagaggat tttatgtttt
     2101 cattattagc aactgccggt acgacagact cttctggcga gacattgaca gatcaagctg
     2161 tcagcaaagt aaatgcgttt cagcggtttt gggacgggat tgattgggac gccattttgg
     2221 ctgcctttat ccaaaaaagt ttatatctga tttttttgat cgtccttttt tggattcttc
     2281 accgtgtggg aaaatatctg attgataagt cttttaatca gtattctaaa aaagccattc
     2341 taaacgagag ccgcttaaag actttacata gtcttttaaa tactattttt cattataccc
     2401 ttggtttctt ttttatctat gcaattttat ctgctatcgg tgtgccgatc ggatcacttt
     2461 tggctggtgc cggaattgca ggtgtggcaa tcggtcttgg tgcccaaggg ttcatgagtg
     2521 atgttattac cggatttttt attatcatgg aacaacagat ggatgttggg gactacatca
     2581 agctagcaaa cttaacgatt gaaggaaccg tcgtatctgt tggtatccgt acccttcagc
     2641 tgaaagcaac ggatggtact gtccatttca tcccgaatcg aaacatcacc acgatcagca
     2701 atctctctcg tgcaaatatg caagtacttt tagatattcg gattgttcct gaagaaggat
     2761 atgacaaaat ctatgagatc attgatcgag taaatcgtac attagctgaa aaataccaag
     2821 aagagctcca aactgaacct tctatctttg gattagtgga tcttggaaac agcaattttg
     2881 cgattcgtac ggtctgctac gcattgaacg gaaaacaata tgccttaaaa gaagagttct
     2941 tgagctccta tgtcaaagaa ttgacagctt ctggtttcac gattccgaac agtccaattg
     3001 ctgtcggaaa ataatttgga caaacgatgc aacgaatctc actaaaaaga gcgtgggaca
     3061 aaagtaaaga acacttttgt c
//
LOCUS       DAZAFF010000175         3071 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000175 DAZAFF010000000
VERSION     DAZAFF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.185"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..273)
                     /locus_tag="ACG9BB_002901"
     CDS             complement(145..273)
                     /locus_tag="ACG9BB_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838751.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            complement(412..600)
                     /locus_tag="ACG9BB_002902"
     CDS             complement(412..600)
                     /locus_tag="ACG9BB_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838752.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            678..902
                     /locus_tag="ACG9BB_002903"
     CDS             678..902
                     /locus_tag="ACG9BB_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFN2838753.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            complement(899..1054)
                     /locus_tag="ACG9BB_002904"
     CDS             complement(899..1054)
                     /locus_tag="ACG9BB_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838754.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(1194..1406)
                     /locus_tag="ACG9BB_002905"
     CDS             complement(1194..1406)
                     /locus_tag="ACG9BB_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838755.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(1409..1621)
                     /locus_tag="ACG9BB_002906"
     CDS             complement(1409..1621)
                     /locus_tag="ACG9BB_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFN2838756.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1896..2306
                     /locus_tag="ACG9BB_002907"
     CDS             1896..2306
                     /locus_tag="ACG9BB_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2838757.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            2319..2771
                     /locus_tag="ACG9BB_002908"
     CDS             2319..2771
                     /locus_tag="ACG9BB_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFN2838758.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            2862..>3071
                     /locus_tag="ACG9BB_002909"
     CDS             2862..>3071
                     /locus_tag="ACG9BB_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838759.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIN"
BASE COUNT         1033 a          521 c          472 g         1045 t
ORIGIN      
        1 ttttgggctg tacaaataat agaaacacct tgcttttggc attcacgttt caattcttcg
       61 ataagatttt caattttctt attcatgtga tataattctc ctagttttat aaatttgtat
      121 gtgactcttt gctggctggc agagtcactt ttttatttgt tgccatgctt tttgcttttc
      181 gatatgttgc ttgcttaaaa taatagggcg gctatttccc caccgattat cagcaatcac
      241 tttaccgatt tttagcgctt cttctcgtgc catagttgct cctttctttt gaatcaagca
      301 gattgattaa aaccatcaat gctgcgaaca agcttccccc gataatactt tggtgcgcta
      361 caatcactaa taaccccaaa atgaatccta taaaaagtgt gtctgtcttc ttcataatct
      421 aatctcccta ttttttattt ctagcattct caaatcctca agttcagaag caattagttc
      481 agcttgccta tctgatagct catcggcttt tctaagcgct gcacggtcat cttgtaattg
      541 tttcctgcgt tgtttaatca aaccgagaat ttgatgttct tgttgcaagg tatatgccat
      601 atcacttctc ctttcgttta ttgaatttct ccatttctat ttgataaaat atttctatag
      661 aaatggaggt gaaaactatg ggaaaaaatc aacatgttgt acctaattcc aatggtggtt
      721 ggaatgtcaa aggtgctgga aattctaaag cgactgcaca tactaataca aaatcagaag
      781 cagtaaaaat cgctagagag atttctaaaa atcaaggatc tgagctattt attcatggca
      841 gagatggaaa aattcaaagc cgtgatagtc acggaaacga tccacaccca ccaaaaggtt
      901 aatcatattt aggagttaat cgaatacgat aaccgtcagc aggagttgca tcggtcaatg
      961 taacttctgc tacttttttc ccttcttctg tttcaataat taatcttgtg taattgttct
     1021 cattcaaaat ccctattttt gattcttcct tcatgtcatc cctccttcgt ctctcgccgt
     1081 caccacctcc aagaagagga gttgccaaaa tgaaaaaacg tggactttgg caaacttgaa
     1141 aggagagcag caactcctct tttcagaagt ggtgaattta gattttttag ctattaacaa
     1201 acagctctaa ctctaaatcg atttttagaa cgccatgttc taaactgttc atataaatcg
     1261 acatcaattg ttatttcttt ggaagtaact gcgttatacc cctcactaaa acgtgatttt
     1321 ttaaattcct tcattcttct ttgataagta ctttcagaat acttatattt ctctttaaac
     1381 tccgctttgc tcaaacgttg taccattttt acgcctcctg ttctgatggc tgatcattag
     1441 aatctacttc taacttgata tccaaaagtt cttcgatttg ttttaaacgt tccttaggtt
     1501 ttcttttttc gatgaagata tctcgtaaat aagtaattga tatacccatt cgtcgagcta
     1561 actctctttg actccaacct ttctttacta aagcaattaa tacttgctgt tttaaagtca
     1621 tgcttcatac ccccttatcg aatattttct aatatttttg tctaataagt ttgacaaaaa
     1681 ctgcaaatat gctattatgt aagcatacaa aatacagcgc acaaaaagcg agcttactga
     1741 tgtctgggga gactattagt ttattgcttt tagtctttgt atgccgtaaa tatcttgctt
     1801 acaaaactag tataactgca aattctctat ttgtaaatag ataatttgca gaaaaatact
     1861 ttttttgtcg gtattttgaa aggaatgcat atcctatgct attagaccgt ataaaaatct
     1921 tattaaaaga acgttcaatg acgttggcag aacttgaaag aaagttaaat ttcggaaatg
     1981 gaagcatgtc tagatggaat aaaagtactc catctggaga taaaatacaa aaagttgcag
     2041 attactttaa tgtatcaact gattatcttt taggacgtac tgataaacgc cattattatg
     2101 atttaacaga aaaagataaa caagatattg ctttacaagc tgaaaaatta cttgaaggct
     2161 tgaattcaga tgctgaaaca aattattatg gtgaaccttt agacgatgaa gataaggaaa
     2221 aattatacga tgccattcaa cttgcacttg ccttaaccaa agttaaagca aagaaaaaat
     2281 tcactcctaa aaaatatcgt caataggtgg gctttggtat gcatttaaac aaatactgga
     2341 tattagaaga aatagaaaaa ttatttgtag agtttggatc tgatgcgatt aatccatata
     2401 aaataatcaa atattctgat atagatttaa ttgaaactga tttagggaat aaaacattag
     2461 gacaaacgat acacaataaa agatgttatg tgatattaat taatcaaaac ctatcacctt
     2521 ctatgaaaag atttgtatta tggcatgaaa tagcccacgt tagactccat aaaggtattt
     2581 gcacagcagc ttttagagca aacaacctta gttgtatgat ttatggtata gaagctgaag
     2641 ctaatatgtt tgcaattgaa atgttaacta gaacaataga tgaacaagat ttatctgata
     2701 tgagtcagtt ccaaattatt gaatatttag ggcttcctca taatcttgaa catttagtat
     2761 tacaacatta aaaataaaaa agcccatgct ttcacatgga ctctttcctc atttctgagt
     2821 aaactctaca ataattatat caaagaaatg aggagttttg aatgttatta ttgattggac
     2881 ttattttatt aattatagga atcactaaac tgtcgaaagc taacacaata aaaaaagaag
     2941 cttatgaatt agaacaaaaa aatgatgaaa aactaaataa attacatgat agagaaagat
     3001 ttatattaag taaggaagaa acactatcag atttaaataa acaaattact atgttaaaaa
     3061 atgatattaa t
//
LOCUS       DAZAFF010000176         2914 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000176 DAZAFF010000000
VERSION     DAZAFF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.186"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1364)
                     /locus_tag="ACG9BB_002910"
     CDS             complement(45..1364)
                     /locus_tag="ACG9BB_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFN2838760.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="ACG9BB_002911"
     CDS             complement(1361..2014)
                     /locus_tag="ACG9BB_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFN2838761.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..>2914
                     /locus_tag="ACG9BB_002912"
     CDS             2302..>2914
                     /locus_tag="ACG9BB_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFN2838762.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          955 a          474 c          320 g         1165 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgaca ccttctgttg
     2101 ccaatacttt ttcttttttc taataaatgc aaacagacac cttgaaatcc tttagaataa
     2161 agggttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgt attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tattgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaaactaa tcgctaagaa gtacttcgtc
     2701 tcggatggga cggtgacacg gatcttgcgt gaagcaacga agcattacca gccacgtatg
     2761 aacttccttc cgacggtgtt gtgtatggat gagttcaaat caatgaagtc tgtttctggt
     2821 tccatgagtt ttatttgtgt tgatgggaca acccatcagc tatttacgat ccttgaagat
     2881 cgcagacttt acaaactaac tcaatacttc ctgc
//
LOCUS       DAZAFF010000177         2799 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000177 DAZAFF010000000
VERSION     DAZAFF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.187"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG9BB_002913"
     CDS             85..972
                     /locus_tag="ACG9BB_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFN2838763.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG9BB_002914"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG9BB_002914"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFN2838764.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG9BB_002915"
     CDS             2274..2720
                     /locus_tag="ACG9BB_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFN2838765.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT          936 a          451 c          608 g          804 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gaactgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtcttt
//
LOCUS       DAZAFF010000178         2675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000178 DAZAFF010000000
VERSION     DAZAFF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.188"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            699..785
                     /locus_tag="ACG9BB_002916"
     CDS             699..785
                     /locus_tag="ACG9BB_002916"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFN2838766.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1087..1311)
                     /locus_tag="ACG9BB_002917"
     CDS             complement(1087..1311)
                     /locus_tag="ACG9BB_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838767.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1677..1961
                     /locus_tag="ACG9BB_002918"
     CDS             1677..1961
                     /locus_tag="ACG9BB_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838768.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1964..2380
                     /locus_tag="ACG9BB_002919"
     CDS             1964..2380
                     /locus_tag="ACG9BB_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838769.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2517..>2675)
                     /locus_tag="ACG9BB_002920"
     CDS             complement(2517..>2675)
                     /locus_tag="ACG9BB_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFN2838770.1"
                     /translation="NPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKI
                     SLRNWLEE"
BASE COUNT          882 a          497 c          430 g          866 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tactcagata aaaagttgtt agtacccttc tgtatccgtt gggagagtaa
      241 ggctcaactg ccccctaaaa agggttctaa aaaatcttta aaaaataaat aaaataaacg
      301 cgcgcgcgcg agcaaaattt cccgccaaaa aatcacccac tttttaaaat aaacaggttc
      361 gcgcatgata ttaaactgcc aatctagctc cctatggtcg ctgtaagaag gagtgatcgc
      421 tacgctctca cattttactt gcagcagtcg cctttaggct ccttcgccaa aaataattca
      481 ttagggactc ctttgtggcg tcctgagagc tcgtctaagc cgttctaaga caagattcga
      541 taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
      601 cccaaggcaa taaaaaactt aggcttattt ttgctttttt attgtataat tgattgtcgc
      661 ctatccgaca gtcttgcgac tggaaggagg tgtgtttcgt gttcactaac tttatttgct
      721 cccttcttgt tgggctttta agcgctttgt tcagttactg gttgaataac cgaaacaaga
      781 agtaaaactt aattaaggcg actcagccca caaaaaaagc cccagagaat ccgacctctc
      841 tggggctttc gtgtgtttcg tgttcactta tttgcaatca aattataaca gaaataaata
      901 tttatttcaa aaatatccta caaaagtcta aaatccgtaa ttatttctta tatgcaacat
      961 ttacaaataa agtcgctctg agagctcgta tttttcgatt taagacgttt tatttagttt
     1021 ttggtgtatt tcattggtcc aagctaaaaa actcttagaa gcgattttag agccatttaa
     1081 acccacttaa ttatattctc catgaaattc gctccatgcg tcaaaagctg cgtctgaaat
     1141 tccgccgtct tcgttcacta gttgcattaa tttttccaac gatcgttttc tttcgggtgt
     1201 catattttta tattccaaca atttttcgtc tgttaaatcg aacttgattg cttgaattgc
     1261 ttgctctaat ttttctaagt tttgaaaata ttcgtaagga aatttttcca ttttttcttt
     1321 ctccagtctc acaatttttc tagaaataaa atcacttctt tgccgtctat actctcaaaa
     1381 aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc atttttaaat ttggttatcg
     1441 attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt gtacccactt
     1501 tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat
     1561 cactttttcg ccatcgtggg tagcttatgt atagcatttt ggcaaaaaac agctatacaa
     1621 atatagtatt tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg
     1681 acattttagt tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag
     1741 aactcacgaa taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg
     1801 aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg
     1861 ataaatttat tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa
     1921 ataatcaaat cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt
     1981 ccaaaaacat gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa
     2041 aaatccagat atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc
     2101 cgataccaac acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat
     2161 ttccatcatg actgaaatca tcgcagaatt tgctgattta tatctaaaag atacaggcgt
     2221 tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg aagctaaact
     2281 aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac
     2341 ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata ggagttattc
     2401 ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag caatcttagt
     2461 cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt ttttatttat
     2521 tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct tgaaaaagta
     2581 aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac tttaagatca
     2641 ttgaagtatt tttttagttc tcgtaatgca ggatt
//
LOCUS       DAZAFF010000179         2633 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000179 DAZAFF010000000
VERSION     DAZAFF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.189"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..626
                     /locus_tag="ACG9BB_002921"
     CDS             120..626
                     /locus_tag="ACG9BB_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFN2838771.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="ACG9BB_002922"
     CDS             643..1542
                     /locus_tag="ACG9BB_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFN2838772.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="ACG9BB_002923"
     CDS             1629..2513
                     /locus_tag="ACG9BB_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFN2838773.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT          958 a          489 c          515 g          671 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat ttt
//
LOCUS       DAZAFF010000180         2630 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000180 DAZAFF010000000
VERSION     DAZAFF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.190"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2309
                     /locus_tag="ACG9BB_002924"
     CDS             <1..2309
                     /locus_tag="ACG9BB_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFN2838774.1"
                     /translation="NINYLTSKYISNFEELIDKLDTKDIVNWLNQDEELTDLLSSVKK
                     ESSASQQSNWSGLYFNSNEYVKYILNKTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYG
                     VSEQSLMELLGQRIVEQLDFRKNMAMFSDDEIRAILVELPKFDKGEISRRLYNELIRN
                     KKGMNPTYSTEGLSVLAKDGFFYPNKELRYADKRLPKSIEKQMRFIYVPEKASTTTIY
                     EWLGVERFKTNLKLETFELLEDYSEDFKKEIDDIKTAVLSTIDDNSKNVSSLKRIQII
                     PCSKISAKDVEQDGQSIVLEDYYFVESKGKFYLKLPANSIEINQLRLIDTFSSTIVDI
                     FKQVLNLSLDLNLIELLISRDTEHKQRKITEEFGVDKWNESFELLFSENALNKKVNDF
                     LLSNGLNEEEYPTIFEIDYSYALKEEEYQLLAIALESISKDIEDLNGLSELIHLDIRE
                     FWKLKLRSYLDKKAEHYRQILYSNANKSGDEILKETFLEKIDRYKDYQFEISEIPNSV
                     QVDIEKESKKRFPELSVSITDEIDIDGLYDNNVEELVTVAAITIDDFDYYIRGHQKVS
                     SLLYFGIPSRLREDILIFVTKENDVDLNGEKSGISIEGTSTVQTILEPSNSVSRKGKN
                     TRNGERSKRDYDNRNSANDDAGETAEQIAYAELTQNQKLNVIWNTKYSSKSSDRNKQP
                     PNGIVCDMWVHDPENGNMYFEVKSSITEFEMSINEYNSMLDNPDSYEVVLVNRDTKEI
                     SRHKFDELDGLKQASSYLFKFKQKRSDG"
     gene            2444..2572
                     /locus_tag="ACG9BB_002925"
     CDS             2444..2572
                     /locus_tag="ACG9BB_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFN2838775.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         1006 a          344 c          500 g          780 t
ORIGIN      
        1 cgaatattaa ttatttaact tctaagtaca tcagtaattt tgaagaatta atagacaaat
       61 tagatacgaa agacattgtc aattggctca atcaagacga agaattgaca gatttattat
      121 cttcggtaaa aaaagaatca agtgcaagtc agcaatcgaa ctggagtgga ttgtacttta
      181 attcaaatga gtatgtgaaa tacattttga ataaaactgc atggattaag attgatggag
      241 aaaaatatag cccgagacaa attgtgctat atgaaaagct ccaggcacac gttccgggat
      301 tgtatggagt ttctgaacaa agcttaatgg aactcttggg gcaacgcatt gttgaacagt
      361 tggatttcag aaaaaatatg gcgatgtttt cggatgatga aataagagct atattagttg
      421 agttaccaaa attcgataaa ggcgaaataa gtcgcagatt atataatgaa cttataagaa
      481 ataaaaaagg aatgaatcct acttattcta ccgaaggatt atcagtctta gccaaagatg
      541 gcttctttta tccaaacaaa gaattaaggt atgctgataa gcgactgcct aaatcaatag
      601 aaaaacaaat gcgttttatt tacgtgcctg aaaaagctag tacaactacg atatatgaat
      661 ggcttggggt tgaacgcttc aaaacaaatt taaaattgga aacatttgag ttattggaag
      721 attattctga ggatttcaaa aaagagattg atgatatcaa aactgcggta ttatctacga
      781 tagatgacaa tagtaagaat gttagttcat taaaacgtat tcagattatt ccatgcagca
      841 aaatatctgc taaagatgtt gagcaagatg gtcagtcaat cgttttggaa gattattatt
      901 ttgtggaaag taaaggaaaa ttctacttaa aattacctgc taattctatt gaaattaatc
      961 aattacgttt gattgatacg ttttcttcaa caatcgtaga catattcaag caggtgttaa
     1021 atctaagctt agatttgaat ttgattgaat tgttaatttc ccgagatacg gaacataaac
     1081 aaagaaaaat aactgaagaa ttcggtgtag ataagtggaa tgaatcgttt gagctattgt
     1141 ttagcgaaaa tgcgttaaat aaaaaagtta atgacttttt gttatcaaat ggactaaatg
     1201 aagaggaata tcctaccatt tttgaaattg actattcata tgcattaaaa gaagaagagt
     1261 atcaattgtt ggcgatagct ctagaaagca tttcaaaaga tattgaggat ttgaatggat
     1321 tgtcagaatt gattcatttg gatattcgtg aattttggaa actgaaatta agatcttatc
     1381 tagataagaa agcggaacat taccgccaga ttttatatag caatgcaaat aaaagcggtg
     1441 atgaaatact caaagaaaca ttcctagaaa aaattgatag atacaaagat tatcaatttg
     1501 agatttctga aattccaaat tcagttcaag tcgacattga gaaagaaagt aagaagagat
     1561 ttccagagct aagtgtgtca attactgatg aaattgacat tgatggcctg tatgataata
     1621 atgtcgaaga gttagtcacg gttgcagcta ttacaataga tgattttgat tattatatta
     1681 gaggtcatca aaaagtttct tccttactgt attttggtat tccgtcccgt ttaagagaag
     1741 atatactgat attcgttact aaagaaaatg atgttgattt gaacggagaa aaatctggta
     1801 tctctattga aggaacatca actgttcaaa ccattcttga accatcgaat agtgttagta
     1861 ggaaaggtaa gaacacacga aatggagaaa gaagtaaacg agattatgat aatagaaact
     1921 ccgcaaatga tgatgctggt gaaacagcag aacaaattgc ttacgctgaa ttgactcaga
     1981 atcaaaaact taatgtaatt tggaatacga aatattctag taaatcttca gatcgtaata
     2041 agcagccacc aaacggaatt gtttgtgata tgtgggttca tgacccagag aatgggaata
     2101 tgtactttga ggtaaaatca tcaattacag aatttgaaat gtcgattaat gaatataatt
     2161 ctatgttaga taaccctgac tcttacgagg tcgttttagt caatcgtgat accaaagaga
     2221 taagtcgtca taaatttgat gaacttgatg gattgaaaca ggcaagtagc tatcttttca
     2281 aatttaagca aaagagaagt gacggttgag tgatttgttt ccacaaacct attcaatcta
     2341 tcaactcaaa aatcataaac aaccattatt atagtaggaa gatacagagt gtactctcaa
     2401 cgcatatcga attagtcgct tcattcgtta aaggagaata aaaatggacc aaaaagaaaa
     2461 aatgaaagaa ttgatcaaaa agaaacaagg tggaggccga tccaaacaaa tggaaacacc
     2521 taagaatgat cgtaaaaaca tgagaaaagg ccctaaaatc tttaataaat agacgtgcta
     2581 aagaaagact aagaagtgtt aacttttgtt attgtttctt agtcttttta
//
LOCUS       DAZAFF010000181         2593 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000181 DAZAFF010000000
VERSION     DAZAFF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.191"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1398
                     /locus_tag="ACG9BB_002926"
     CDS             <1..1398
                     /locus_tag="ACG9BB_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFN2838776.1"
                     /translation="GEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGN
                     YEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLP
                     ALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYN
                     PEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGS
                     SDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEK
                     VDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEP
                     NAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSS
                     NKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLG
                     TLGLLGYKNRKKENE"
     gene            1532..>2593
                     /locus_tag="ACG9BB_002927"
     CDS             1532..>2593
                     /locus_tag="ACG9BB_002927"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFN2838777.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESI"
BASE COUNT         1015 a          361 c          521 g          696 t
ORIGIN      
        1 ggcgaaaaag tagaagttgg gggcacagta gatctaacag ataatgttac taacttgcca
       61 gcgttaccag aaggaacaac agtaacagat gttactcccg gcggtacaat tgataccaac
      121 acaccaggta attacgaagg tgtcattgaa gtaacgtatc cagacggtac gaaagatacg
      181 gtgaaagttc cagtagaagt gacagacaat cgttctgacg ccgataaata tgaaccaaca
      241 gtagaaggcg aaaaagtaga aattggaggc acagtagatt taacagataa tgttactaac
      301 ttgccagcgt taccagaagg aacaacagta acagatgtta ctcccggcgg tacaattgat
      361 accaacacac caggcaatta tgaaggtgtc attgaagtaa cgtatccaga cggtacgaaa
      421 gatacggtga aagttccagt agaagtgaca gacaatcgtt ctaatgcaga taaatacaat
      481 cctgaaggac aaaaagtgac tacagactta aataaagagc cagatgcatc tgagggaatt
      541 aagaacaaag aagatttacc aaaaggaact acttatgctt ggaaagagaa agtagatgtt
      601 agcacagctg gaaataaaaa aggtactatt gtagtgacat atccagatgg atcatctgat
      661 gaagttgaag tagacgttac agtgacagat actcgttccg atgcagataa atataatcct
      721 gaaggacaaa aagtgactac agacttaaat aaagagccag atgcatctga gggaattaag
      781 aacaaagaag atctaccaaa aggaactact tatacttgga aagaaaaagt agatgttagt
      841 acagctggaa acaaaaaagg tactgtagta gtaacatatc cagatggatc taaagaagaa
      901 gtagaagtta ctatttctgt agtagataaa aaagcaccga ataaacctca agttgatcct
      961 attacagagg gtgaccaaat tgttactggt aaaactgaac caaatgcaga ggtgacagtt
     1021 acattaccag acggaagtca acaccatggg acagctgata aaaatggtaa ttttacagtt
     1081 aaagttccta aattagaggc agggacaaaa gtgatagtaa ctgcaactga tgaatctggt
     1141 aatactagtg aacctactaa tgtagttgtt tcttccaaca aaaaagacag tggaaaaaac
     1201 ggtagtaaag gtagtaagac tgacaatcaa ggtagcaaca gcaatcaaga caaaaataga
     1261 ggcaaaagcc agtcctcaaa agtttttcct aagacgggag agagtgacag taatatcttt
     1321 actattagcg gtggtttaat actgttagga acgttaggat tattagggta taaaaatcga
     1381 aaaaaagaga atgaatagaa aatcaatttc ttaagtcttc tctgagaaga ggaagaatct
     1441 ttttcttcct ctttttgctt ttttatgatg ggaaataagt gttagttaaa gatgaatata
     1501 ctttttatag aaaggaaaag aatgtaatta agtggaaaaa atagtaatta ttggttcaaa
     1561 ccattcaggg ctagccgcag ctcgatttct aaacttaagc gattcaaatt atgaagttat
     1621 tctaatcgat aaaaaatcag aaattagtta cttggaatgt gggacatcgt tatttataaa
     1681 aggagaagta agtaatttag aagatctttt ttatcaaaat agaatgacac taaattctga
     1741 gataaaaaag ttatttctag agacagaagt tgacaaaatt gatattcaaa ataaaaagat
     1801 tagttgtgta aaaaaagatg gtacaagatt ctctcaagaa tatgacaaag ttatattagc
     1861 tacaggttca aagcaaatta tgctagatat accggggaat aatttagcta atattttttc
     1921 gataaagaat tttccaaatg caaaagagat ttttaaagct ttggatgata gtactattaa
     1981 aaaagtagga attattggag caggatatat tggagttgaa attgctgaag ccataagaaa
     2041 aagagggaaa gaagtatatc ttttcgatgt tgcagataga gtgttgtcga cttattacga
     2101 ccgtagtttt tcagataaag ttgaagaaat actatcaaaa aatggaatac atctttgtct
     2161 atcagaaagt cctaaaaaat atttaggaag aaaaaagatt gaaaaaatag tcactacaag
     2221 tggtacccaa tactctatgg atatggtcgt acaagctata ggatttctat ctaatagtcc
     2281 aattggggaa aaggaactct tgagagatgt tagtggagca tatctaacta atgaatacca
     2341 gcaaacaaat ataaaagata tatatgctat tggggattgt gctacaactt tttttacgtc
     2401 cattaataga agtagtgttg atttttctat ttctaatgct ctaagaacag cttatattgc
     2461 aactcaacat attaacaatc aagattttac accttctggt tcccaattta caaatggctt
     2521 ttctatattt aattacaatt tttacgcagc aggtttgaat ttaaaaacag cacgattatt
     2581 aagtgagtct ata
//
LOCUS       DAZAFF010000182         2553 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000182 DAZAFF010000000
VERSION     DAZAFF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.192"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..2484
                     /locus_tag="ACG9BB_002928"
     CDS             43..2484
                     /locus_tag="ACG9BB_002928"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFN2838778.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          847 a          457 c          605 g          644 t
ORIGIN      
        1 tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga agtcgttatg
       61 atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc atttactgca
      121 gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact gaaaaaagat
      181 aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca aaatgttgcc
      241 agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac gatcaatgaa
      301 gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca attaggtgaa
      361 gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat gctgaaacag
      421 tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa cgacggattg
      481 actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc aacacaattt
      541 tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa agcagcacat
      601 gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc tgatgaagac
      661 gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact gggcacgtta
      721 cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc gttgaatgac
      781 ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa tctgccttat
      841 ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat gggaatcgct
      901 ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc aacaatcgat
      961 ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca agaactgcag
     1021 ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca tccaattggt
     1081 gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga acagaagaga
     1141 tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt gttagaagcg
     1201 aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct tacgccatct
     1261 gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc gttgattttt
     1321 caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca agtgagtgaa
     1381 gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa aaaatggatt
     1441 ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc aaaaatctgt
     1501 ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt tctatcagga
     1561 aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga gctgattctt
     1621 tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca agatatctct
     1681 actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt agtggatgta
     1741 aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc aaacaagtta
     1801 cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt tgctacagaa
     1861 tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa caaagaaatc
     1921 ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct tgacccttac
     1981 cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt ggcagctaca
     2041 ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag aaatggatta
     2101 taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa actaacggtg
     2161 cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc aaaagcatct
     2221 ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc tgcagtaggg
     2281 ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca aaaaggccac
     2341 atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga ggaaatcgag
     2401 caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc tatacaaata
     2461 ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa gcaagattta
     2521 cagttatttt atttactgca gatcttgctt ttt
//
LOCUS       DAZAFF010000183         2494 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000183 DAZAFF010000000
VERSION     DAZAFF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.193"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG9BB_002929"
     CDS             <1..435
                     /locus_tag="ACG9BB_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFN2838779.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG9BB_002930"
     tRNA            complement(759..842)
                     /locus_tag="ACG9BB_002930"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG9BB_002931"
     CDS             1370..1870
                     /locus_tag="ACG9BB_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFN2838780.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACG9BB_002932"
     CDS             complement(2060..2404)
                     /locus_tag="ACG9BB_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFN2838781.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT          778 a          478 c          413 g          825 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaa
//
LOCUS       DAZAFF010000184         2438 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000184 DAZAFF010000000
VERSION     DAZAFF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.194"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACG9BB_002933"
     CDS             316..570
                     /locus_tag="ACG9BB_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFN2838782.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACG9BB_002934"
     CDS             571..888
                     /locus_tag="ACG9BB_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFN2838783.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACG9BB_002935"
     CDS             1447..2382
                     /locus_tag="ACG9BB_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838784.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          884 a          345 c          458 g          751 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagagaaaaa
     2401 tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DAZAFF010000185         2409 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000185 DAZAFF010000000
VERSION     DAZAFF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.195"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1384
                     /locus_tag="ACG9BB_002936"
     CDS             <1..1384
                     /locus_tag="ACG9BB_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838785.1"
                     /translation="CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIA
                     EAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVR
                     LFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEAL
                     SISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNL
                     FPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLL
                     VTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHE
                     VKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHG
                     LVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTK
                     KEINKKNENK"
     gene            1371..>2409
                     /locus_tag="ACG9BB_002937"
     CDS             1371..>2409
                     /locus_tag="ACG9BB_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFN2838786.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQ"
BASE COUNT          797 a          365 c          416 g          831 t
ORIGIN      
        1 ttgtacgatt ctgattagtt tttattatgg agaaaagttt tttaaaaaga tagatgctgc
       61 ttttttattt agttgtttat atacttttgc aacttatcga acagttgata tctattatcg
      121 atccgcaatt gcagaagcaa tcgctcttac gattatccca gctgttttat attatgctta
      181 tggattgata ttcaatagag aaaaaaaagt catcgcttta tctgtcagta taagtctact
      241 tgtgtatact catgtcttaa ctacactatt gtgtagtgca ttgattgctt gtatgattat
      301 agttcgtttg tttttaagca agcaaataaa aaacgatata aaatttgtgg tcgtgaattt
      361 agtaaaggct gtttttttga cttgtgcatt aactagctat ttttggtatc ctatgttgca
      421 gcagatgaat tatcaagaaa tacacagtcc cttcaaaaca gatttgcagc tagaagcact
      481 ttctatttca gatagtatca ttcaagcgtt aaataacgat attacaacat acactatggg
      541 gttattagga ttggttgcgt tgatttttcc tttattcata ctgaaagaaa tggagaaaaa
      601 agaaaaaatc atctatgttg gcgtagtttc tacatgggta tgcgtaacta atttatttcc
      661 atggtttttg tttcaaaaaa caccgataag cttactccag tttccttggc gtatcttagg
      721 tatccaaatc tttttttctt caatgatcat aacgcttgtt tttatgaaaa agactatgaa
      781 taaaaaatat tcatggttaa tcattggcgc tactgtggtg tttctactcg tcacgtctat
      841 agcagcaaaa gaaaattacg caaaagttat ccaatcaaaa cacggtcata ttgttatcaa
      901 tgaagaaaca ctcccacgtt atacaacatc agatatggga ggattatacg attacgcccc
      961 aacagaagcg ataaaagaaa ggcctcactt agaaaaacat gaagtaaaag taggagaatc
     1021 gtgggaaaaa gggaagtata aagctgcaga taataacatt acttacacag tggcaagcaa
     1081 caaaaagcaa aaagtgacag taccagtata tgcttattta ggtacacagg taaaagtaaa
     1141 cggtaaagtc gtaaatgact cttataaaaa acatggctta gtgaatgtgg atgtaaatgc
     1201 tggagaaaat aagatagaaa ttacaacaaa atatactcca actgctctca tggctttatg
     1261 gatcagtatt ctgacttatt gtttagtggt gtattcaagt tttaaacgat ttgcacctaa
     1321 taagttgaaa gattctcgat atgtgactaa aaaggagata aacaagaaga atgaaaataa
     1381 ataaaaaagt agtaatattg agtggattaa ttatagttag tagtatatat tctttcatct
     1441 ttttcaaaaa tacacagagt atttatacca ctggagatct ttcttttcac ttatctcgaa
     1501 tcaaggggtt atcgactatt tttaccagtc cgattaatta tgagacattc aattatactg
     1561 gatatggtgt aaattatttt tatccctttc tgactttttt tccagctgta atgttatatt
     1621 ggatgacaaa aaatttgatt gtaagctata ttatttacgt ttggttattg aacttgtgta
     1681 caacactcgt agcttatcat tatggcgagc ggtttttaaa acaaaaaaaa gcggcatttt
     1741 tatttagttg tctctatatt ttctctgctt atcggacagt agacatctac tatcgttccg
     1801 caattgccga agctatcgca ataacacttg ttatccctgt attattttat gcttatcaaa
     1861 tcatctctgg aaaagaagaa aagtatccgt ctgtaaagct agcattgagt atgagtttgc
     1921 tagtgtacag ccatgtacta tcaacattaa tgagtacagc attaattatc atttttattt
     1981 ttattcgttt ggtttctaaa ggtttcaaga atgcagattt tattgctata tttaaaaaat
     2041 tatttagtgc ggcaggaatg acattggttc tgacgagtgc tttttggtat ccgatgtttg
     2101 aacaaatgct ctaccaaaaa atcaacaaac ctagcgtgac aaatttatat gctcatgcat
     2161 ctaatgtgtt cgatagtctg actgaagcta tgaacaacga cttaactact tactcaatgg
     2221 gactagttgg tctgttatct ttatgtattc ccctgatttt atttaaaaaa ttaacaaaca
     2281 tagaaaaaaa gatttattat ggtacgtgtt tgacttggct agctacaact agtttggtgc
     2341 catggtatct tctgcaaaat acgccagcca aattgttaca atttccttgg cggatcttaa
     2401 gcttgcaaa
//
LOCUS       DAZAFF010000186         2400 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000186 DAZAFF010000000
VERSION     DAZAFF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.196"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG9BB_002938"
     CDS             207..2069
                     /locus_tag="ACG9BB_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFN2838787.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ACG9BB_002939"
     CDS             2069..>2400
                     /locus_tag="ACG9BB_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN2838788.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZAFF010000187         2345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000187 DAZAFF010000000
VERSION     DAZAFF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.197"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..397
                     /locus_tag="ACG9BB_002940"
     CDS             <1..397
                     /locus_tag="ACG9BB_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFN2838789.1"
                     /translation="LVIKKEQGFTGTETKSLFLKDKKDNNYVYLTFTTKQTDFKALGK
                     IVGKRLSIVSSERMEEQTGQKQGAVSPFGYEKQIPIIIDEELLSNEKLVFAPGRPDQT
                     MVIQTVDLNKIIQLLEMPIFLMPANEKNN"
     gene            434..1444
                     /locus_tag="ACG9BB_002941"
     CDS             434..1444
                     /locus_tag="ACG9BB_002941"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFN2838790.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1559..2272)
                     /locus_tag="ACG9BB_002942"
     CDS             complement(1559..2272)
                     /locus_tag="ACG9BB_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838791.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT          786 a          390 c          483 g          686 t
ORIGIN      
        1 tttggtaata aagaaagaac aaggatttac aggtacagag accaaaagct tattcttaaa
       61 agataaaaaa gataataact acgtatatct gacttttact acgaaacaaa cggatttcaa
      121 agcattaggg aagatcgtcg gtaaaaggct ttctattgtt tcatcagaac gcatggaaga
      181 acaaacggga caaaagcagg gcgctgtttc tccgtttggt tatgaaaaac agattccgat
      241 tattattgat gaagagctct tatcaaatga aaaacttgtt tttgcaccag gtagaccaga
      301 tcaaacgatg gtcatccaaa cggttgactt aaacaaaatc atccagctac ttgagatgcc
      361 gatatttttg atgccggcaa acgaaaaaaa caattaacca agtaaaatag tgcatacttg
      421 gaggtgaagc aacatgataa gttatccatt attagaaaga aatgcaacga ttggggttac
      481 tgcaccatcc tcaggcgtat cagaggaaca ccattcgtta ctcgaatcag ctttaaagag
      541 aatggaagaa aaatctttcc aagtagttac tggaaagacg ctttggacgc aagataagga
      601 aaaatcaggt ccagcaagaa aacgagcaga tgaattcaat caaatgatgc aagatgaaaa
      661 tatccaaatg atctttccgc cttggggagg agaattattg attgagattc tcgattttgt
      721 cgattttgat gctctacaac caaaatggat agtaggttat tcagatatta gtacgttgct
      781 acttgctgtc acattaaaaa cagggatagc cactgcacat ggaaccaacc ttattgattt
      841 aagaggagag tacttggatg aaacgacagc aatgtgggaa agtgtactga aaacaagaga
      901 aggagaatcg attctacagc aaccttctga aaagtatcaa aaagagtggc cccatgagca
      961 ttcatcatct tctgttttcc atctcactga accaacaaat tggcaatcag tcagcggaga
     1021 tgcagtaagg atgaagggac gattattagg tggctgtatt gatacgatca gtcatttaat
     1081 cggtacccct tatggagatg tccaatcttt taaaagaaag tataatgatg gaaagccgat
     1141 cttgtggttt ctagaaaatt gtgagctatc gtcagttgat ttaagaagat cacttgttca
     1201 gatgaaactg gcaggatggt ttgatggcag ctcggggatc atgttcggta gaagtgcagc
     1261 agaggatttg ttagattata aaatgaagga tgtatatgaa gatctgtcaa aggaattaaa
     1321 actaccgatc ttgtatgata ttgattgtgg tcatgtaccg ccacaactga cattgatcaa
     1381 tggtgcctat gcagaagtag cggtagaaga tagaaaagcg actgttttgc aaacgtttat
     1441 ttaaaaaatt ttttcctgcc ttggagttta tcaaagacca acaaatctaa atagaaaaga
     1501 aaatgaaaga agctgacagt attttgccag cttctttttg tatgatctat tagaaatttt
     1561 aattatccat aactatcaca ttgatcagct gtttcacatc atttggatta cctgtaggaa
     1621 tagctactac agatgcatta tcaataattt cttgagttgt ttctctaatg aacgctggat
     1681 aagatgtttg atctgcaggc atttgccaat gtgaaggaac tggataacta gtgattgtcc
     1741 cgtcatggtt acttgaatac actaaaactc cctggtaact gtatttccca gtgagcataa
     1801 ccacatcttt cggatagact gcactccctg catcataagg ggcgataggt gttcctgcag
     1861 gataatgaat aacatgtagt tcgaaatcac ccgatgtatc tctaaaagta ttacctctaa
     1921 ttgctagcca aacacgtgcg tactcaattt gtagatcaga atatcctgct aacggatcta
     1981 ttgactgaga agactgtgag gaagttgtgt ctattgttga agaagaaata tttgtctcaa
     2041 ctgtactact agatgtagaa gaagtattgg gctctgagac tttactgctt gttgaagatg
     2101 tattcgtttc agtagaagct gatttctgag tacttgattt ttcagctgaa ctggtttgtt
     2161 tgattgaact tttgtctgtg gtcgcagatg gatcactatt ttctttacta ggagacgtac
     2221 atcctacaag gaataaagag accaacaata aaataaaacc taactttttc atataaaata
     2281 cctcattatt tttttaatac cagtatagca taatcctgaa tttttgaatc cgtaaaacct
     2341 tgtct
//
LOCUS       DAZAFF010000188         2235 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000188 DAZAFF010000000
VERSION     DAZAFF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.198"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..1685
                     /locus_tag="ACG9BB_002943"
     CDS             72..1685
                     /locus_tag="ACG9BB_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFN2838792.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          695 a          398 c          444 g          698 t
ORIGIN      
        1 ttttccggca caattatttg ctgtttttta ccataacata gtacgatcaa cgtagaaaag
       61 gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc tggcggtcct tcatttgcca
      121 ctcgtttccg tcgattgaat gaagcgcacg aaatcattat ctatgaacgc ggagaaaata
      181 tttcttatgc aagttgtgct ttgccttatt atttaggagg tgtgatcact gaccgtgact
      241 cgctgatcga acgtacacca gaaatattga aaacaaaaaa caacatcgat gtctttacta
      301 aacacgaagt aacagcaatc gatccttcta ctaagcgatt aacagttaaa gacctatcca
      361 caaatgaaga aataaaaaca gattacgata agttgatcat ctcttctggt gctagaccag
      421 attatccgga tattcccgga gtttttgaag cagaaaacgg ctttgtcctc cgtagtgtga
      481 cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa tccacaacat gtcgtcattc
      541 ttggtgcagg tgttatgggt ctggaattag ctgagaattt caagcatcgc ggcttagacg
      601 tgactttaat cgatcaattg ccacaagtcg ctttccctta tgatccagaa attgctaatt
      661 tagtttatga caaattgctg aaagaaggat tagccgttca tttagaaaca agagttactg
      721 agatccgtga taaaggtcga gaaatagtat tatcagatgg ctccgtcctt tctgctgata
      781 tgctaatttt tgctgttggt gtttctccga agaatgaagt ggtgaaagca accggcatac
      841 aattatcaga tacaggtcag atcattgttg atgaccagtt acaaaccaat ctgccggaca
      901 tctatgcgat tggcgatatt atcgaaacga ctagtgtagt gactggtcag ccgatccaaa
      961 gtatgctttc cagtgcggcc aatcgtcaag gacatatgct ggcagatatt ttaaatggta
     1021 cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc aaaaatcttt gatcatacag
     1081 caagttatgc gggaatgaca gaacatgaat taaaagcatc aggcataaca aattataaaa
     1141 ctgtttttat cactcccttt gaccatgcct atttctatcc agaagctaca agattaaatc
     1201 taaagctgat ttttgatgca gatagcggtc gtattttagg tggacaagca tttggagaaa
     1261 aaggtgtcga taaacggatg ggagaacttt ctgtagcaat caccggaaac ttgacagtct
     1321 ttgatttgcc tgatttggag ttgccttact ctccaccgta ttctactacc cgtgacccgt
     1381 tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa tatcgtagaa acgatcaaag
     1441 caagtgatat acccgaaaac gatttgaaag aagcgttctt tttagatata cgtgaaccta
     1501 ataaagcacc ttctggcagt atctcggcaa caaaaaatat tccaatgaat gagttacgag
     1561 atcgaatcaa tgaaatccca aaagataaaa aagtttacat tactttcaga agaggattga
     1621 atacttatac ttctgcccga atcttggcag gtttgggtat caaagcgatt ttgattgaag
     1681 aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag ttatttttac ttgaggagta
     1741 aaaatgaaaa agtatatagg gttgttggtt ggtatgtata tagaatagaa gataaatgtg
     1801 aagaaattgt gcattatttc ttcacattta ttaggttata gtttaagaaa gtttaaacat
     1861 tgcgcaattt aattatttta gctgtttctt ttcataaaaa aacatgttgt gactctgaga
     1921 caaatcatag tccatagtga tgtctctaga gtaactacgc ctaatattac gaggcatcat
     1981 attaatctat cagcactagt acctaaacta gcatcccttt cgaatatttt ttcttatata
     2041 ataaagtttg ctttcactcc tctaatctca ccaaatcgac tggcttgcgt tgtgtgatac
     2101 taggaactga ttggtttaga acgacttttc tattgtaatc atatttttcg ccatcctcgt
     2161 tgtcttctaa tccttcaatg aatgatccct aatgtgtgta ttaaataatt taattttttt
     2221 gttcctattt attta
//
LOCUS       DAZAFF010000189         2118 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000189 DAZAFF010000000
VERSION     DAZAFF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.199"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..244)
                     /locus_tag="ACG9BB_002944"
     CDS             complement(<1..244)
                     /locus_tag="ACG9BB_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN2838793.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
     gene            complement(237..1829)
                     /locus_tag="ACG9BB_002945"
     CDS             complement(237..1829)
                     /locus_tag="ACG9BB_002945"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFN2838794.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
BASE COUNT          620 a          430 c          328 g          740 t
ORIGIN      
        1 tgattaattc attatatttt ttttcagtaa ttttatatct tgtttcgtaa ttatcataaa
       61 tagcattttg ttgttcatag acaacaaaat tgctattaga tacaaagaat tctttcttta
      121 aggcactccc aaatggtccc ctccttactc catctttttt atcaagtaag cattcaaact
      181 tacgctgttc ccaagcttcc gtaaatcctg ggaaacgaat ttctggttgt atatcattac
      241 tcatgtttga acacctccag cgcaccttgt aaccacgctt cattttcagg tgttgtttgc
      301 agggaagaga tagcttctaa aagttctttt tctagcactt gctgttcttg tcgtatcttc
      361 ttgatgtcat ttccgacatg aaccatatca attggttctt cttcttcaaa cgtatctaca
      421 tagcgtggaa tatttaagtt atagtcattt tctttgattt catcaaagtt tgccaaatga
      481 gcgtatttct cgacatcttt tctttcagca taggtttcga ggattttttg aatgttttct
      541 tccgataatt tattctgatt ttttcctttg acaaattctc gactagcgtc aatgaataat
      601 acgtctcgag tttggcggtt ctttttcaat acaatcaccg tcgttggaat cgatgtcccg
      661 aagaacaagt tcgcaggcat accgatcaca gcataaatac tgccgtcttc tagtaatttt
      721 tgacgaatga ccccttctgc agcgccacgg aataaaactc cgtgtggcaa gacgatggcc
      781 atagtccctg tttccttcaa atgatagaaa ccatgaagaa gaaacgcaaa atctgctttg
      841 gattttggcg ctaattttcc ataacgatta aaccgagaat cgtctaagaa ggtcgtatct
      901 gctgaccaat tagccgaata aggtgggttc attactactg catcaaatgt gtaaggttca
      961 tctgttggcc aatccttatt caacgtatcc ccattgcgga gattcatttc ttcggcatct
     1021 accccatgta aaatcaagtt catttttgct agattgtatg tcgttgtatt taattcttgc
     1081 ccatgatatt taacgttatc tgggtgagtt aagtagttac gcacatttaa catcaatgaa
     1141 cctgaaccca tggttggatc aaaaacacta aagaatcgtc gttctttttg atccaacgtc
     1201 acgatttgtg ccatcatatc agaaaccata tgaggcgtgt aaaattctcc ggctttcttc
     1261 cctgcttctg aagcaaattg actgatcaga aattcataag catccccgat cacgtcccca
     1321 tcgtgaccta aaacttccac gtcattcaat ttcttaatga cttcggtaat cgtcacattt
     1381 cgttgttgct catctgttcc tagttttttt gattggagat ccacatcatc aaataaccca
     1441 ttaaattgat tgtaggtaga tgccaattgc acaaaggctt tgtttagatc attcaattgg
     1501 aacgttgctt gttttgcttg atccgctaag acgttgaaaa gatactctgg cgaaatcgca
     1561 taccccaaaa tatcaacgat tgttgcaatc agatcttctt ttgattcttc atctgaaagt
     1621 aattcgcgat acaacatcgt ttgtttgctt accgtatcat attcctctaa ggattcatct
     1681 gctaaaagaa ccacttgttc caacaaacgg tcggataaat atttatagaa aatcagtccc
     1741 aataaataat ttttatattc tgacgcatcc attttgctac ggagattatc tgccgcacta
     1801 aataattttt gatttaattc tgcacccaaa aagatacact cctatctctt atttaaagtt
     1861 gctatatacc ctcttatttt agcatactcc cttttagaaa aagcaaaaaa aaaaagaaag
     1921 aaacttattc ttgctaataa actacgcgaa aggagtctaa cgttgtttga tataacttag
     1981 actgatcgtt aacttatttt ttatatttct acatagttat ctttagaacc atttcagata
     2041 catatgaaac aattacatat gattttttca taaaaaaaca gtactgtcta gtagtataaa
     2101 atatacaaat ttcttaaa
//
LOCUS       DAZAFF010000190         2088 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000190 DAZAFF010000000
VERSION     DAZAFF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.200"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(131..418)
                     /locus_tag="ACG9BB_002946"
     CDS             complement(131..418)
                     /locus_tag="ACG9BB_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN2838795.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(589..1941)
                     /locus_tag="ACG9BB_002947"
     CDS             complement(589..1941)
                     /locus_tag="ACG9BB_002947"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFN2838796.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT          575 a          417 c          359 g          737 t
ORIGIN      
        1 aaaaaaaggg gttgacaaag cttgtcataa cccctttttc tgattaaaag atagtaaaga
       61 gacagtcatc ggtaattgtg atgatttttt gtcatcactt aaggatgagc ggccttttca
      121 aaaactgtct ttaaagatca tcatcagttt ctagcacata tggctgacct tcatatgtgt
      181 actctgtatt caatgtgccg tcattttcat ctgagtagat gcgtctactg aaaggaaaac
      241 gatgagaaac attatctttg gcgtcatttg ctggcatcgt gtaatattcc tcttgcgttt
      301 cattcaactg agagagtact tgatctgcca actcatcaat attgatcgtt aatttcttcg
      361 aagcactttt ataatcttct aaaaatgcat cttgttggac aaaatattct ttattcatct
      421 tctttctcac ctttcttttc aaaatcaaat aaagctgctt gttcttttat gtaacagctt
      481 tatctttgct gcttattgtg acaaaaaatt cgaagaaaga caactaattg tacttttcac
      541 aaaaaaagac agagggtcgt gacaaaattg tgtcacaacc ctttttcttt attgtgcagt
      601 ttcgatttcg cctagcgctg cttgtgcaag taggttcaag taattccatg ggcggtcaaa
      661 atgtggttgg aagaagaaat ctgaaagtgc gagatcttct acagtcattt tattttgaac
      721 agctaaagac atggtatttg ctgattgtgt aatgtcgtat ttggacatga actgagcacc
      781 tacgatacga ttcgttcctt tttcatagac tagctccatc attacttttt cagtagttgg
      841 catgaactct ggacgataat tatcttcaaa tacagtcgct tggacttcta gatcgttcaa
      901 agttgcgctt tctttggtca cacctgtcga accgatcgtc cagccaaata ggtaaagtcc
      961 tgaagttcct tgagtaccac gatacgccat tttatgatct gtcaagttat ttcccacaag
     1021 caaaccttga cgaacggcat ttgttgccaa aggaatatag ttttgcgtgc cacttggatt
     1081 gtagttgact actgcagagt ctccagctgc aaaaatgtct ggattacttg tctgcatgta
     1141 ttcattcact ttgattgccc cgtttggcag tgtatctact tttccttcga tcagttttgt
     1201 atttggacgg aagcccacac ataaaatgac catatctgta tcaaatgtat ctgttggtgt
     1261 agctacttgt ttcacttttc cttcttcatc tgtgataaat tcagtaacat tttcgcctag
     1321 cgcaagagta acaccgcggt ctgtcaattc tttttctaag atatctgtga atggtttatc
     1381 tagatattta ttcaagatgc gatctaatcc atcaatcaat gtcacttgtt ttccagattc
     1441 gacaaaagct tctactaatt caataccgat atatccacca ccaacaatta ctacacgttt
     1501 tgcttctttc gcttgtgcaa taatttcatt tgcttgattg tagtttttgc ataggagtac
     1561 atttttcgca tcgattccac taatcggtgg gataataggc catgaaccag ttgtcatcac
     1621 taatttatca taagaaaccg tttctgtttc tcctgttttc agatcttttg ccgttactgt
     1681 tttattttct gtatcgacgt ctgttacatc gtgttccatt ttaactgttg ctcctaatga
     1741 agataattct tcaggatttg agtagaacaa gcctgctggg tcctttacta ctccgccgac
     1801 ataaagtgcg ataccgcaag ataagaaaga aatattgtca tttctttcat atactgtcac
     1861 ttctgcttct ggatgatttt ttaaaatact ttttacagct gcagtacctg catgagtaca
     1921 gccaattact actactttca tttatggtcc ctccgattaa cattttgtcc ttcgacaaaa
     1981 ctactataac agaaataaaa cggatacaac agtttaatgc ttgtgaattc acaaaggttt
     2041 gtttgtagtt tcattccttt tcaagcataa gaacccaata tctacaag
//
LOCUS       DAZAFF010000191         2051 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000191 DAZAFF010000000
VERSION     DAZAFF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.201"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>2051
                     /gene="acm"
                     /locus_tag="ACG9BB_002948"
     CDS             <1..>2051
                     /gene="acm"
                     /locus_tag="ACG9BB_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFN2838797.1"
                     /translation="PVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKKQN
                     IQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNLDS
                     VEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGDML
                     PEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRGEE
                     GIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENHSE
                     AWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFELR
                     RADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKFSI
                     NENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAETK
                     RLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNENKE
                     VISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYRD
                     GQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREKI
                     RNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVIIGL
                     L"
BASE COUNT          804 a          338 c          404 g          505 t
ORIGIN      
        1 tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac
       61 cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga
      121 aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa
      181 tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg
      241 aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct
      301 agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg
      361 tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt
      421 gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct
      481 acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt
      541 tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac
      601 gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat
      661 ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta
      721 cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa
      781 gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa
      841 agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc
      901 agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa
      961 tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc
     1021 tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa
     1081 aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt
     1141 tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt
     1201 cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa
     1261 cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa
     1321 ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac
     1381 ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga
     1441 agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct
     1501 agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga
     1561 caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg
     1621 tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa
     1681 tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc
     1741 cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac
     1801 aaatatatct ataccaggta gccatataac accgtggaaa aattttccag agaagacaga
     1861 gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga
     1921 atccagcgaa caaagaagca attatattag taagagagct gtacctaaac agataacaaa
     1981 taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat
     2041 tggtttattg a
//
LOCUS       DAZAFF010000192         2050 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000192 DAZAFF010000000
VERSION     DAZAFF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.202"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..259
                     /locus_tag="ACG9BB_002949"
     CDS             <1..259
                     /locus_tag="ACG9BB_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFN2838798.1"
                     /translation="EIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMP
                     ESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            260..1012
                     /locus_tag="ACG9BB_002950"
     CDS             260..1012
                     /locus_tag="ACG9BB_002950"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFN2838799.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1017..1964
                     /locus_tag="ACG9BB_002951"
     CDS             1017..1964
                     /locus_tag="ACG9BB_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFN2838800.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          719 a          271 c          403 g          657 t
ORIGIN      
        1 agaaattcat ttagattatt ttggaagagt tcctacaaga aaatttcaat tgtttacttt
       61 agatgaatgt atcaatgttg acttattaaa taataaaata agttttgaag aaaacattgt
      121 taaagaaatg cctgaatctg ctaatgatat gtatgagaag gagttgaaat atttctttga
      181 aaacgtagta acaggcaaag aaaactggaa tgatttatat catgcaaacg aagtattgaa
      241 aatagctgag gagaaataaa tggaaatttt atttactatt tgtggtagag ctgggtctaa
      301 ggggtttaaa aataaaaatt tgaaatattt ggcaggaaag cccctagtcc atttcgcttt
      361 agcaatgata gatatctatc aacaaaaaca tcctgaacat aattgcacga cttgtttgaa
      421 tacggacagt aatgagttag tagaattgac gactaagtta aatccgtctg tgaaaattgt
      481 agaacgtaaa cctgaattag ctacagatac tgtagggaaa attgaagtga tacgtgattc
      541 attcttacaa ctagaaagag aagataatat atttgattta attatagatt tagatttgac
      601 ttcaccttta cgtgaagttt ctaatcttga agaattaata gatgaacatc taaaaaattt
      661 agataaagac gtctactttt cagttgtttc tgcaagacgt aatccctatt ttaatatggt
      721 aaaaagaatt gaaaatgaag ttactatagt taataaatca agatacacat ctaggcaaca
      781 gacacctgaa gtttttgaaa tgaatgcctc aatgtattct tttaaacctg aatttctaaa
      841 aaaaagtaat tatatttttg atgggcaatg tgggataata aaaatgagag attatctaat
      901 tttagatatt gatagtgaag aagattttaa atggttagaa tttttgtttc ctaaattttt
      961 agaagattct tcggagcttc aagatatcta taattattta tatcggaggt aaaaggatga
     1021 gctgttatct tatcaccggt ggggctggct tcattggttc taccttagcc aatcatttag
     1081 gagaagaaca tgtggtaatc gtcgtcgatg acttatcaat gggaaaagta gaaaatcttg
     1141 atatgacgaa aaatatcact tttattgagg gagatgtcgc tgagaaatcc ttgatggaag
     1201 agatcatgaa agctaataaa tttgattata tctttcattt agcagcagtt gctagtgtgg
     1261 cagattcagt tgcccgacca gtagaaacac atcgtgtgaa ttttgaaagt gtattaatga
     1321 ttttagaatt gatccgcaag tatcaacctg atttaaaacg gatcgtgttt agttcttcag
     1381 cggctgttta tggggatgaa ccgactttac caaaaaaaga agagtccgtg attcgcccat
     1441 tgacgcctta tgcggttgat aaattcgctg cagaacaata tgtgctagat tattgtcatt
     1501 tatatgatgt acccggtagc gcagttcgtt ttttcaatgt ctatggtcca aatcaaaatc
     1561 cgaattctcc atacagtggt gtgatttcta ttctcgtaga tcgctataag aaacaaatcg
     1621 taggagaagc aacttcattt acattatttg gtgatggaag ccagtctaga gactttgttt
     1681 atatagatga tgtaatccaa gcattacttt tagttgcgaa tgaggaagct gcactgggga
     1741 aacaattcaa tgttggaacg ggtaaagcaa ccacactttt ggaattgatt catgcaatca
     1801 atgaaattct cggtgtagaa ttgacacttg aatacaaaga agagcgttcg ggtgatatcc
     1861 atgactctct agcagatatt actaagatcc agtcaattgg ctatcaaccg aaatatggta
     1921 ttttaagtgg aatgactaat tatttgaaaa ctgaaatcaa ttaaaacata ctaagataag
     1981 attagagggt aaattcaggc tctttgtcaa taaggactga tgagttttgt aaaatcaaat
     2041 ctgggataga
//
LOCUS       DAZAFF010000193         1998 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000193 DAZAFF010000000
VERSION     DAZAFF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.203"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..366
                     /locus_tag="ACG9BB_002952"
     CDS             40..366
                     /locus_tag="ACG9BB_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN2838801.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            367..1116
                     /locus_tag="ACG9BB_002953"
     CDS             367..1116
                     /locus_tag="ACG9BB_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFN2838802.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(1196..1651)
                     /locus_tag="ACG9BB_002954"
     CDS             complement(1196..1651)
                     /locus_tag="ACG9BB_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN2838803.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT          650 a          307 c          394 g          647 t
ORIGIN      
        1 taattacaac gacagatgga gtaatatgga ggaaagaaaa tgatttcaag tgatggaata
       61 cgcggataca atgatgcgat tattttatcg atccttaaca aaaacgattc ttatggatat
      121 gaaatttcta aaacgattcg tgagaggaca aatgattcct atgtgatcaa agagacaaca
      181 ctttactccg tttttgcccg tttagaaaag aatcaactga ttgtttcgta tcctggagaa
      241 gtgactcatg gaaaaaagcg gacatattat aaaatcactg aaatagggcg taatctttta
      301 gaagaaaaaa agcaagagtg gctgacgacg aaagacgtag tcgatcaatt tttggaggga
      361 aattaaatgg atacaatccg taattattta gacagtcttt ttatcggagt acctcaaagt
      421 accgagatag ataagctgaa aactgatttg ttggcaaata tggaagacca ttatcatgaa
      481 ttgatgggag aaggaaaaaa tgaacaggag gccatcggta cagtaatttc gacatttggt
      541 tcaatcgatg aattattaga agaactagat gtcgaaaaaa aacatcaagc tgacgagacg
      601 gaaacaaaca cagcttctat ttatttaagc gaagcagaaa actactggaa agaatacaga
      661 gcggcaagtc tccaagtggc atcaggtgtt ctattcatta gcttatcttt tgcaagtttt
      721 cttttctttt gttcagcagg atatgtgttc atgggtattt cttgtctgat tttcggtata
      781 gcattagctg tcggtttttt tattgcttct ggtatgaaaa tcacacgttt aaatcacttt
      841 ttgcaccatc ggaaaatacc tgaaaaagta ttagcagaag caaaagaaaa agaagaagag
      901 tatcagcgaa gttttggctt ctcactgatt gcaggaattg gattatgtat tttttcactt
      961 ttcccacttt tggcttcttt aatgtggtat atggatggta gtatcggtgc ctctatattt
     1021 tttgttacgg taggaacggg tgtcttcttg atcatttatg gcagtctagt ccgtcattct
     1081 tatcgtcaat tcactcaaag cgcttattac tggtgaatgt attcagattt gtaaaaatat
     1141 tctatttatc cggcgtagaa aaaaagactg tgacacgctt ttgtcacagt cttttttata
     1201 tataataatt taaaaattca tcatctaaat ccgtagaatt ttctagattg cttacccatg
     1261 taaaatcgtt atagacagat ttcagttgac ttctgcgcca ttgtacaggg ataaaaagac
     1321 gcatgctatc ttcattgaat ccgactaata atttctttcc gtcagtaatg atgggacgtt
     1381 gtaacaaagt aaaatgcgag acgatcagat cgacaagctc tgaggtcgta cattggtcga
     1441 tttggtcttt caaattttta taatcaatcg agcgtttagc taatagatcg tcaaatccgt
     1501 tttctgttaa tgacaataaa taaaatatct ctttccttgt taaaggctgt ttaggtgtga
     1561 aaattcgttc tctgtaaggc atttgatggt ctttcagcca gtttgccatc agatggtaac
     1621 tatgtgtgcg agaagaatag atcgtaagca taaaaaaact ccttaaatag tccattaatt
     1681 tgttagatct gttgagataa ataataggta aggagctgtg cataagcatt gatcaaaaca
     1741 acccaaacat cattagaaat aactttctaa agtcttaaac ttgcgacgag aagcaactag
     1801 actaatagaa aaaatgtctt agccatttgg cttcctgttt gtttttttgc ttatgctgca
     1861 gctcctcggg aaagggttct aacaatttga agaaggttga tgattgatct agtttaaatt
     1921 gtgtgaaatt tgaaatctgg tgtttttact agatgaatca cctaacaact atgattataa
     1981 cttatagaac ttctgttt
//
LOCUS       DAZAFF010000194         1972 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000194 DAZAFF010000000
VERSION     DAZAFF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.204"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..1202)
                     /locus_tag="ACG9BB_002955"
     CDS             complement(24..1202)
                     /locus_tag="ACG9BB_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFN2838804.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIE
                     SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE
                     "
     gene            complement(1300..1758)
                     /locus_tag="ACG9BB_002956"
     CDS             complement(1300..1758)
                     /locus_tag="ACG9BB_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2838805.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKLTRV"
BASE COUNT          559 a          337 c          329 g          747 t
ORIGIN      
        1 ttgtcctcct gcaaaataat tagttactca gtaaacattg aaactaatgt atcggttacc
       61 tgttgaaaac ctttatggct tctgtttaga aatttttgat tgtatgtatc aaaaatgctg
      121 actagaaagc gttctagtga ttcttcattt tgaaactgct cttttctacg gctgtatctt
      181 ttaatttgct tattgaaaga ctcgattaga ttggttgagt aaatggttct acgaatgcta
      241 ggtggaaaat cataaaaagt taataagtct tggttttcta tgagtgactg cgtcacttta
      301 ggatagtttt tcttccattt ctcaatcatg ccggataaga aggtattcgc ttcttctttt
      361 gagttagctt gataaacagc cttaaagtca tcacagattt cttttcggtc tttgacacgt
      421 actttatgag cgatgttacg agatacatgg atacaacaat gctgatattt tgctttagga
      481 taaatttgat ggatagtatc tttcatgcct tttaagccgt ccgtaataaa aagcaagact
      541 tcttgaactc ctctggagtt aatatcctgt agcagctcat tccaaacgta tgttgattca
      601 gttggagcaa tcgcataact cagtacttct ttagtgccgt cttctcgtat accaatggca
      661 atataaatcg cttctttgga tacggtttga cgttttagtg gaatgtaagt agcgtccata
      721 aaaatagcga catacttatc atttaaggct ctggatttaa aggcatttac ttcttcagtc
      781 agaactttag tcatgttgga catggtttgt ggagtatagt gatgaccgta cattttttcg
      841 atcaaatcag caatttcaga catcgtaaca cctttttcga ataaatggat aatagtggtt
      901 tccaatgtat cgtttgttct tttgtaggct ggtaaagttt gttgtttaaa ctcaccatta
      961 cgatctctag gtatttccaa tgttaattca ccatattcgg ttttgattga tcgaaagtaa
     1021 gaaccgtttc tcgaattacc tgaattaaaa ccagtgcgat catatttttc gtaatctaaa
     1081 aaagccgtta attcagtccg taggagtgtg tttatcgctt tttctaagtg cgaacggaat
     1141 aattcattta aatcgccttt agtgactaga gtttgcacaa tttctgtagt aaaatcattc
     1201 atagggaagt cctcttttct gtgaattggt tgtcgttaac tttattctac agaagggact
     1261 tccttttttg tatggatttt ttcatttaca caaaatattt tatactctcg tcaactttct
     1321 cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta atactttata
     1381 agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga tatatttttc
     1441 ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac gagataacag
     1501 agctttgcta gaacattttc cattaatttc cagacatata tccaattcac tgacggttac
     1561 tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat cgatcgtttt
     1621 tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg actctgataa
     1681 atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt ctttgcttaa
     1741 aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt ccatgcattc
     1801 accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt cagcgattct
     1861 tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc gttttatttg
     1921 gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat gc
//
LOCUS       DAZAFF010000195         1962 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000195 DAZAFF010000000
VERSION     DAZAFF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.205"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            351..620
                     /gene="rpsN"
                     /locus_tag="ACG9BB_002957"
     CDS             351..620
                     /gene="rpsN"
                     /locus_tag="ACG9BB_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFN2838806.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            635..814
                     /gene="rpmF"
                     /locus_tag="ACG9BB_002958"
     CDS             635..814
                     /gene="rpmF"
                     /locus_tag="ACG9BB_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFN2838807.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            846..1223
                     /locus_tag="ACG9BB_002959"
     CDS             846..1223
                     /locus_tag="ACG9BB_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838808.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1244..1393
                     /gene="rpmG"
                     /locus_tag="ACG9BB_002960"
     CDS             1244..1393
                     /gene="rpmG"
                     /locus_tag="ACG9BB_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFN2838809.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1417..>1962
                     /locus_tag="ACG9BB_002961"
     CDS             1417..>1962
                     /locus_tag="ACG9BB_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFN2838810.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLS"
BASE COUNT          718 a          287 c          392 g          565 t
ORIGIN      
        1 aaaattggcg ataaaccagt taaacataaa agattgaata aaaagttata gtttctttca
       61 tattgccgtt ttaagtagag gaaaagctac ttaaaacggt atttgtgttt gattggacta
      121 ataaaagtga attcttattt ttcagagcct aaactgcata ttcttttaaa tgatttgatt
      181 atctctatgt accgataaat tcgttttcaa atagttatct attcatatga gttttatttc
      241 atgttattta ttgttgacaa gagcaactct tgttggtaaa gtatcagtgt caaaacgtaa
      301 cgattacgat ttgaggtgtc taaatcaaaa taaaaggagt ataaaggtat atggcaaaaa
      361 agtcgaaaat tgctaaagca aagaaacaaa tggcgatgat tgaaaagtat gcagataaac
      421 gtcaggaatt aaaagccgca ggagatcgca ccgctctcgc taaattgcca agagattcaa
      481 atccgaatcg tctgaggctt cgtgatcaga cggatggtcg tccaagaggg tatatgcgaa
      541 agttcggtat gtcccgtatt aagtttagag aacttgccca tcaagggttg attcctggag
      601 taaaaaaagc aagttggtaa aaggaggaaa tatcatggca gtaccagcaa gaaaaacatc
      661 taaggcgaag aaacggttaa gaagaactca tcaaactgta gtaaaaccag aaatttcatt
      721 tgacgaagcg aatggtgatt atagacgtag ccatcatgta tctttaaaag ggtactataa
      781 tggaaagaaa gtaattaaag gagcttcaaa atagtttaat atataacttt taggagggaa
      841 tcaaaatgga aattatttac ccacctttag ttgaagaagg attaaagtat tatcttgaaa
      901 cgacccagca atcgctagat aaatgcactt tttatcgttc gatggtagag agaggaatta
      961 ttactgaaac gggtttaccg acccaacagg caattgaaaa tggattagtc aaagattact
     1021 atgaggacca aggcttatca tttgatgatt ttttaagaat ctatccaatt tttgaagagt
     1081 atgacgagga gttatttcaa tgtatcgatg gttattggga gatacctgtt gatatgaaag
     1141 aaaatttagt ttcacaattg gaatctggcg agttaacttt tgaagatgca caacaaattc
     1201 aagcatatct tgaagatcga taagtattga aggaggaaga tttatgcgtc aaaatattat
     1261 tttggaatgt gtcgaaactg gagaacgtct gtatctcacc agcaaaaata aacggaataa
     1321 ccctgaacgg ttagaattaa aaaagtattc gccaaaattg cgcagacggg caatttttaa
     1381 agaagtgaaa tagctaagtg gtaaaaaggt gacaaaatgg gcataccagt tacaatcgtt
     1441 tctgggtttt taggggctgg aaaaacaacg ctaatcaatc aagccttgca agtctcaccc
     1501 taccccaggg aagaaattat tataattgaa aatgagtttg gtgaagttgg gattgatcac
     1561 aaattattag ttcatagcga ggaacgggtg ttacaactaa ataatggatg tatgtgttgc
     1621 agtcttcgca gtgatttatt agcaatattg atttctttac tggaaacaat agaagaacat
     1681 caacaaccaa tttcacaaat catcattgaa actacaggta tagcagatcc ccaaccaatc
     1741 attcaaacac ttttaacagc gcctcaacta agaggaagag tatatatcga tagtttattg
     1801 actgttattg atagtgacag ttttgaactt attgaaagag aaccacaagc ccttaagcaa
     1861 cttgtcatgg cagatcgcat ttttgtgtct agaaaacaaa acttgaattt atctgcacta
     1921 aaaaaattac agagagaaat caaaagtatt aaccctttat cc
//
LOCUS       DAZAFF010000196         1922 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000196 DAZAFF010000000
VERSION     DAZAFF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.206"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..249
                     /locus_tag="ACG9BB_002962"
     CDS             <1..249
                     /locus_tag="ACG9BB_002962"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838811.1"
                     /translation="IKKYRKDTLKSFRINLPNKFIGGFKMSHTLLIKDTLNILDLNIN
                     IHFEENCLTKEKYKGQICLIYKGTLQYTPEEYPHLPIY"
     gene            276..542
                     /locus_tag="ACG9BB_002963"
                     /pseudo
     CDS             276..542
                     /locus_tag="ACG9BB_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(590..709)
                     /locus_tag="ACG9BB_002964"
                     /pseudo
     CDS             complement(590..709)
                     /locus_tag="ACG9BB_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
BASE COUNT          691 a          364 c          258 g          609 t
ORIGIN      
        1 ataaaaaaat atagaaaaga caccctgaaa tcctttagaa ttaatttgcc caacaaattc
       61 ataggaggat tcaagatgtc tcatactctg cttatcaaag ataccctaaa tattctagac
      121 ctaaatatca atatccattt cgaggaaaat tgtttaacta aagaaaaata caaaggacaa
      181 atttgcttga tttacaaagg gactcttcag tacacaccag aagaatatcc acatcttcca
      241 atatattaaa agaaccttct tgaatcattg agtgaaagaa aagagattcg tcactatttg
      301 aggactgttt aactagagac attagcgatc tgcctgaaac ctatacaacc actttacgaa
      361 cgtttaaaaa ttttctgcca cagattcaaa atgcgcttaa ctgcagctat tcaaacagac
      421 cattggaatg tttaaacaac cacatcaaag tattaaaacg caatacgtat ggctttcgca
      481 gcttttacaa tttcaaacta cggatcatga ttcgtcatgg caaaacattt ttaaccaaat
      541 aaacagaaga atgtgggata aatctaaaaa tcagttttgc tacagactct cgagtaaacg
      601 gcgggagcag aagcaactcc ttcggaaata agccgaaatt cacaaaaatt tgaaaagcaa
      661 ttttcgtgaa tttcttctta tttctcggag ttaaacactt ctgtcccagc ctcttctgtc
      721 caaattattc taagatttct ctttaccaac atcatttgac aaagagcttc ttttttgaat
      781 actatattcg ttctgttttt catcttcaaa tacgaactca tccgcagaaa tatttgtcac
      841 aactccatga atcttctttt cattaccacc tatataccac aggattccta cacagaaaca
      901 acaaaaaaat agcataaaat aggttttctt tattttatat ccctcctttt agattaaaag
      961 ctcacagaaa acgctttcta aaaagacaat agaataatca taatttttca ttttgtatta
     1021 tttatacaaa taatacgcat gttactgaaa aatgatatgg ttccctactt tttgagataa
     1081 gttaaataaa atctgtcatc gataaaacag ctagtacagt aatgataaat agtaagaact
     1141 taaaatattt tggattttta tttttgaaat aatcgaagaa aagtaaaagt gataacgaaa
     1201 tagcaaacaa gattatatct ataagattat tcattgagaa atcgccccat atggaactct
     1261 attttgacct gacacaccga gacataaagc aatcatatcc tctgcattgt tttatactat
     1321 tagaactata gctacaccta ttggatgcta ctggctctaa gattaatgct aatacagtaa
     1381 catcaatacc taaactagca gaaactctat ttgcatcttc aatttcagat gcctggactg
     1441 tcgtgactag cgccgtagta gaaaaattta ctggtactaa caaagatgca gacacgactt
     1501 ttatttcact tatcctttat cccataacct aaacttcaat cattacttgt gagctgatca
     1561 atgttcttcc aagtctgaaa gaacattgat cagcttggca aacaaataat gttttcaaat
     1621 acttgatcga tgaaaaaaag ctattggtaa taacagtaat tgcattttcc attttcgtgt
     1681 cagatacttc gcttacagct ggtaaacgat caatcccatt gtcaaatttt ggattttttt
     1741 ctgatgatgt cttgctttag ttcataaaat aatccagcaa taaattcctc gcaaggatag
     1801 ccaacttcat tttcctaatc ggaattttta aacttatcgg tatagataca gatgcaatga
     1861 gaacaaacat ttatttcgag tcaccatcta aaatattttc ctccatgtac ccgttcatta
     1921 tt
//
LOCUS       DAZAFF010000197         1792 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000197 DAZAFF010000000
VERSION     DAZAFF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.207"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACG9BB_002965"
     CDS             complement(<1..613)
                     /locus_tag="ACG9BB_002965"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFN2838812.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSI"
     gene            complement(585..1481)
                     /locus_tag="ACG9BB_002966"
     CDS             complement(585..1481)
                     /locus_tag="ACG9BB_002966"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFN2838813.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
     gene            complement(1471..>1792)
                     /locus_tag="ACG9BB_002967"
     CDS             complement(1471..>1792)
                     /locus_tag="ACG9BB_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838814.1"
                     /translation="ILDKCHFGLNLFKSNTIIGLSLKSIEYFRFGLPIISNLSGDTKE
                     IVKRYNVGINYNSTVDENEMLKFYQCNKNINENVRKIYATIFSEQVLKMKWNEVYKVM
                     NDEK"
BASE COUNT          621 a          310 c          219 g          642 t
ORIGIN      
        1 caatagagaa taggagcaag ctaaggattg taacatatcc agtaatcttt ttattaattt
       61 ttacaataaa aaaagggaga taaaaatatg caagattatg gaacagtcca gccactaaga
      121 taaaaaaaat atacttaaat atagctttgc tgcctcgcaa tttagttaga tgataaaaag
      181 ctagtaatat gaatatcaat gcgctaaaca tacgtacttg tattgcttgt gaagtaaaac
      241 caaaaataaa aaacaagaac aaaactaaaa aaacattagt tgtgaataat ctgatgacat
      301 acaataaagc tgagagtaag acagtagaaa taatcaataa aaaaatctga tactgactgg
      361 aaataaaggt acatacgtac ataacagctc tgaatcccgg ttcaatacta tctgaactaa
      421 agtccatacc aaattgtgct atcccattat acaagacaga atatgcataa taatcagcgt
      481 tttgtgtgtt aaatgccatg atgatccaca ttatcaaaag tagcacccca gacaaaatct
      541 tgttcgatgt actccatttt ttttgtgaaa cactcttttc ttgattaatc tccaatgacg
      601 cctctgtatc catctcgaac cccccttata gccgctctaa atctactgag cttatcagtc
      661 tcaaaagcta ttgcaataaa aaatttcaat aatcttttat tcataaggcg ggaaaattcc
      721 ttatcacttt tatacattct tttcaccaat gttgaatttc ttgcaataaa atactttcgt
      781 gttgaattat gattgtctat tttaattttt tttgatccta tttttaaatt ttctccatct
      841 ccaacgtgat gataaaaata gacttgattt gttacataaa tgttatagcc attatttgca
      901 acccttttac aaaattcaac atcaatctga tcaataaaaa attcatcctt catatttcca
      961 acatcgtcaa ttaattcact tttgaaaaac attccagagg aatttgcaaa tgctattttc
     1021 atggaaccac tagttgaagc aaagtgtttt tttacgtatc tccctctctc atttttgcag
     1081 gtaaaagaga cagattcatc attgctattc tcaaagtaat tgggaacaat aatcccaatg
     1141 tccttatctt tcgcttcaat attggtaaac tcttctaata attttaaaga aagattcttt
     1201 tgtatgatgg tatcttgatc tagtaaaagg atataatcac atttctcttt tttggcaatt
     1261 tcaataccta tgttttgagc tgtcgcaagc ccaatgttct cattcatttt ttttaaaatt
     1321 aagttttcca tcatttcaaa ttgaaaagag ttaatattat ctgagccgtt atccacgata
     1381 ataagtttgt tctgtttctc ttgtattaac gtatccacta ataactttat gttattatct
     1441 ggattgtagg taacaataat tatcccaatt ttatttttca tcgttcatca ccttgtacac
     1501 ttcattccat ttcattttca atacttgctc agagaatata gtggcgtaaa tttttctaac
     1561 attttcattt atgtttttat tacactgata aaatttcaac atctcatttt catcaacggt
     1621 actattataa ttgattccga cattatatct tttaactatt tctttcgtat ctcctgataa
     1681 atttgaaatt ataggtaatc caaacctaaa atactcaatc gatttcagac ttaaaccaat
     1741 tatggtattt gacttaaata aatttagtcc aaaatgacat ttatcgagta ta
//
LOCUS       DAZAFF010000198         1733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000198 DAZAFF010000000
VERSION     DAZAFF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.208"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..233
                     /locus_tag="ACG9BB_002968"
     CDS             <1..233
                     /locus_tag="ACG9BB_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN2838815.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
     gene            392..1687
                     /locus_tag="ACG9BB_002969"
     CDS             392..1687
                     /locus_tag="ACG9BB_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN2838816.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          562 a          335 c          313 g          523 t
ORIGIN      
        1 tattaaaaaa tcttcctgaa acacttgatg cacaacttcg cacgaaactt caaaaccttt
       61 taacgtatga agaaggtatt tacaatgcta tgatttaccc gtattctaat ggcaaaatag
      121 aggccaagat tccccatatc aagacactga aacgattatc ttatggattt aaatcatttg
      181 agaacatgaa aatcaggatt tttttgatca atcaattgat tcaagtgaaa taacaaaaaa
      241 atctgagaca gaaggtcagt ccttctatcg gctctatact ttttgatgtt ggtgaacttc
      301 accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc tttagaatta
      361 atttggctac caattcatag gaggattcaa gatgtctcat actcagctta tcaaagatac
      421 cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta aagaaaaata
      481 caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag aagagtgttc
      541 ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa ccgttcggtt
      601 gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac aacgatttaa
      661 atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa aacactgttt
      721 cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata cttccatgac
      781 ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca tgaagcgatt
      841 ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct ttgatgaatt
      901 caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg gacaaagcca
      961 tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct atttttctcg
     1021 gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt atgcgcctta
     1081 tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc gtttccacat
     1141 cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca cggtacgact
     1201 taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct attggaaact
     1261 cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg ccagttttaa
     1321 agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc ccaacttacg
     1381 ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag atcagtcact
     1441 atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata caaccacttt
     1501 acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca gctattcaaa
     1561 cggaccattg gaatgcttaa acaaccacat caaagtatta aaacgcaatg cgtacggctt
     1621 tcgcaacttt tacaatttca aactacgaat tatgattcgt catggcaaaa catttttaac
     1681 caaataaaca gaacagaaag aggttttctt ctttctgttc tgtccaaatt att
//
LOCUS       DAZAFF010000199         1675 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000199 DAZAFF010000000
VERSION     DAZAFF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.209"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG9BB_002970"
     CDS             complement(<1..182)
                     /locus_tag="ACG9BB_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFN2838817.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG9BB_002971"
     CDS             517..951
                     /locus_tag="ACG9BB_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFN2838818.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG9BB_002972"
     CDS             974..1294
                     /locus_tag="ACG9BB_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838819.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG9BB_002973"
     CDS             1435..1626
                     /locus_tag="ACG9BB_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838820.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          634 a          252 c          356 g          433 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gtttt
//
LOCUS       DAZAFF010000200         1667 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000200 DAZAFF010000000
VERSION     DAZAFF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.210"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACG9BB_002974"
     CDS             <1..1604
                     /locus_tag="ACG9BB_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFN2838821.1"
                     /translation="ETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHK
                     MFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPI
                     REGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYN
                     GDINNRLARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFS
                     RTNRTYPEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQA
                     YGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQ
                     IQLLEEQIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTY
                     AANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQAS
                     DNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFK
                     YPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          614 a          269 c          342 g          442 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagtaaag tggaagtgta gaaaata
//
LOCUS       DAZAFF010000201         1664 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000201 DAZAFF010000000
VERSION     DAZAFF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.211"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG9BB_002975"
     CDS             131..505
                     /locus_tag="ACG9BB_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFN2838822.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG9BB_002976"
     CDS             complement(619..1248)
                     /locus_tag="ACG9BB_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFN2838823.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG9BB_002977"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG9BB_002977"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZAFF010000202         1577 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000202 DAZAFF010000000
VERSION     DAZAFF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.212"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(497..1111)
                     /locus_tag="ACG9BB_002978"
     CDS             complement(497..1111)
                     /locus_tag="ACG9BB_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFN2838824.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          527 a          291 c          252 g          507 t
ORIGIN      
        1 atttcattgc aaaaaaaata ataaacactt ataaatatcc attatctatc cttcaatttt
       61 tgtgtatttt atttctatta gaaaaaggta tcctgttgga caattttgtt atatcaggta
      121 aacttcattg caataatgat aacaaatgta ctataattac tttgtaaaga gttttgggga
      181 agactctttc atcaacgtgt attaagagac aagaaagaat ccatatggat tctttcttgt
      241 ctctttttcc actacttttt atttttctga cactacatat tcttataact catttctcat
      301 aactgtgaaa caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga
      361 tgatccatct taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat
      421 cgttttcttt ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt
      481 gtcaacatgt ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat
      541 acaaaacttg ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat
      601 ctagatttgg caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat
      661 cagcaaattc aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat
      721 caaatccagt aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga
      781 tgattgccgg gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag
      841 aaagctgatc aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg
      901 gattttcaat caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta
      961 caccacttag tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg
     1021 cgatccaagt gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt
     1081 ggtgataata gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca
     1141 tgaatagaaa cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa
     1201 cacatgtggt ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc
     1261 ttgacatagc aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt
     1321 ttcaccctag attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg
     1381 atcggtcagg gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt
     1441 cagggagcct ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc
     1501 caagtgcaag caagaaaata caaaaaaaca ttggtatgat ttatatatat ataaataaca
     1561 taaaaaaagg aatggct
//
LOCUS       DAZAFF010000203         1535 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000203 DAZAFF010000000
VERSION     DAZAFF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.213"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..1490
                     /locus_tag="ACG9BB_002979"
     CDS             24..1490
                     /locus_tag="ACG9BB_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFN2838825.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          422 a          289 c          326 g          498 t
ORIGIN      
        1 tttaaagaat tggggtatag attatggaaa aacagcaaga caagggcttc ttcgggcagc
       61 cgaagggatt gtcaactttg ttcttcactg agatgtggga acgatttagt tattacggta
      121 tgcgtgcatt acttatctat tatatgtacg atacagttgc caacggggga cttggattac
      181 cacgagcaac agcattagct atcatgtcga tctacggatc tttgatctac atgtctagta
      241 tcgtcggcgg atgggtatct gaccgagtgt taggagcaaa acgcactgta ttcttcggtg
      301 gagttttcat tatgcttggc cacatcgtct tggctttacc atttggtgta agtacattgt
      361 ttgtttcgat ggcattgatt attatcggaa ccgccttttt gaaaccaaat gtttctggca
      421 tggtcggaca tctttattca aagactgact tgcgaagaga tgcaggattt tcgatttttt
      481 acatgggtgt caacttaggg gcattattag caccactaat cgttggtaca atcggtcaag
      541 aatataacta tcatttaggg ttctctttag ctgctattgg gatgttcttt ggattattac
      601 aatacatcat ccaaggacgc aagacattag acggagtggg tacaacaccg ccgaatcctt
      661 tgacaactga agaacgtaaa ggattcatcc gtaacttgat catcgcactt gttgttatcg
      721 ctgcagtatt cggcggggca caactaactg gtcatctgac aattgacttt tttgtcaata
      781 caatcagtgt attggggatt atcttaccgt tgtattactt tatcaaaatg ctgacttcaa
      841 aagatgtaac tgctgaagaa aaaccaagag tatgggctta tatcccatta ttccttgcag
      901 caatcatttt ctggtcatta caagaacaag gttcatctat cttagcttta tttgctagtg
      961 aacgtacaca atctacgcta tttggtgtaa cgatccaagc aagctggtat caatcattga
     1021 atccaatttt catctttatt ctgacaccag tattcgtaac gatgtggacg aaacttggtg
     1081 aacgtcaacc atctacagta gtgaaattct ctcttggact tttatttgca ggattatcgt
     1141 acttgctatt gatgttgccg ggtatgcttt atggtacaag tggaaaagta agtccattgt
     1201 ggttgttggg aagtttcttc atcgtggtta ttgctgaatt gtgtctttca ccagttggtt
     1261 tgtctgtgac aacaaaatta gcaccacgag catttgaatc acaaacaatc gctatttggt
     1321 tcttagctga tgcttcttca caagcaatca atgcacaaat tgcacgcttc tacacaccgg
     1381 gaacagaatc tgcttacttc ggtattatcg gtgcagtagc aatcgtcggt ggtatcttgc
     1441 tatttactgt gaaagaacca atcaaaaaac taatgggctc tattcattaa tcaatgaaca
     1501 taaaaacagg aattgcttat ttgcagcagt tcctg
//
LOCUS       DAZAFF010000204         1530 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000204 DAZAFF010000000
VERSION     DAZAFF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.214"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(270..569)
                     /locus_tag="ACG9BB_002980"
     CDS             complement(270..569)
                     /locus_tag="ACG9BB_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN2838826.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(575..805)
                     /locus_tag="ACG9BB_002981"
     CDS             complement(575..805)
                     /locus_tag="ACG9BB_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838827.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1064..1177)
                     /locus_tag="ACG9BB_002982"
                     /pseudo
     CDS             complement(1064..1177)
                     /locus_tag="ACG9BB_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          536 a          248 c          226 g          520 t
ORIGIN      
        1 aaaccactgc cccataagtg tgttacgctt taaactcttt tatatttgaa gccattaaaa
       61 agcataccat atttttgaaa aaaagtgaga aaaaaggctt ataattggag tggtagttaa
      121 ttagtgactt atttttgatt ttatagcact gatactataa aatatagata tcatcatatt
      181 acacaatctt aatactaact taaaaaatat ctcctttcat aagtatggtg ataaaatccg
      241 ttccgggcta cctttttagg tagcctactt taatctttgt atctttctgg atcaacgaaa
      301 gtatacttta tatagtcata acgccgatga tcgcttcgag cgtctagcac atcagtcacg
      361 atatcaaaca aaaaatatac gtctttcttc attctagttt tcgcagcagg gattttaaag
      421 tagttcttat tagaatagta gagattaatt aataagctat cttcgattgc taaaaagaaa
      481 acttctgaat cccacacctt ataaaaatct ttgacaaatc tattcgaagg atcaaattta
      541 aaccataatt gcgtctcatt aaaaagcata accattactc cgatctattt ttaaacttag
      601 tctctacctc taatatatat cgagttttta ttttgccttc agagaatact gtttcttttt
      661 ttgcagttac aggttgttta ttttcggaaa aagctaatat agctaaaatt gaaacatcca
      721 tctggaattt gtctttttcg ttgctttgct cataaaaatc tgcatattca tcactaatat
      781 tttttctaat aaattcttcc atcataaaga tcacctcgaa aagagtatac gaacaaacgt
      841 tctttttgta aaggtggaat ttattatact aaataaatag gtgaacaagt acttgtgcca
      901 aattatgtgc caaaaaaaat cgaatttaat aaaacacaaa caaaaagaaa tcctattatg
      961 ataacatttc ttataataac aaacacaaca gaagacgtgt aatagttagt caggaacgta
     1021 caaataaccc ctgtatcctt tgcggtacag ggggttattt tgtataagta gacattgtag
     1081 tttctaaact gctatgtcct aaacgtttcg atacgctaag tatatttact ccttgataaa
     1141 gtaaaataga tgcatgcgta tgtcttagtc catcaataaa cagtcgaaga gaaaataggg
     1201 aaaaattgat tactaaagaa aaacttacta ggtcgacggt gtagttgatt ttcatacttc
     1261 aatagattcg aattaaacga tttcgattgg cttataccag tcctatccaa cgaaagatat
     1321 atcgttctta actctagcgg tataacttga agctttacgt aaagccaatg ggaatgaact
     1381 ggagatgact ttttattatt tttagtaata gctgatgggt taatacttac aactttcctt
     1441 ttcaaagtac catataaaaa aacggtgggg ttgtttagcg aaataaagga tctaagccaa
     1501 tagcttagat cctttattct atgctttata
//
LOCUS       DAZAFF010000205         1480 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000205 DAZAFF010000000
VERSION     DAZAFF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.216"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACG9BB_002983"
     CDS             64..972
                     /locus_tag="ACG9BB_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFN2838828.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="ACG9BB_002984"
     CDS             972..>1480
                     /locus_tag="ACG9BB_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838829.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DAZAFF010000206         1469 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000206 DAZAFF010000000
VERSION     DAZAFF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.217"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..1444)
                     /locus_tag="ACG9BB_002985"
     CDS             complement(17..1444)
                     /locus_tag="ACG9BB_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFN2838830.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          580 a          249 c          168 g          472 t
ORIGIN      
        1 gaaagctgtc ttcatattaa ttctcctttt ttaaatacaa gtaagaaatg cttcccaaat
       61 aataccagaa aacaattgaa atgacatcgc gcttattgaa aagtacatga gcctccacca
      121 tttcatctat aaaaatagat agtataaaaa ttaaaacaat accaagaaac gcataattca
      181 ctttacttct aaaaaatgtt ttgagtaaac taaagatatt taacacatag aaagcataga
      241 tacatataaa agcaattata ccacttgata caagtacttg aagaaatcca ttatgcatgt
      301 ttccatgtgc tctttttagt tcattcacat ctgtagtatc aagatctact tttttcaaaa
      361 attttgcatc gctattccca tagaaatcgg tctctccata accaaataac aaagttggtc
      421 ttataacttt aagcgcagct ttccaaatcg taaaacgacc agctgaaatt tcggagttat
      481 cactagaatg ctgtaatatt acttcttttt ctggaagtgg agttttatct gattcatttg
      541 actctataac ttctactttt ctatcaatcg atacaggaat tgttgctaaa aaatccttag
      601 cataattagt accaaaagaa aaatatacca atagaactaa taatatgctt accatccatt
      661 tgatttttac aggtagtact tctctaatgc tctttctaaa ttttgaattg gcaaacaaca
      721 aaagaaataa tactaaaaaa gaacaaatta ctacctgggc acctctagac gaagaaagtg
      781 taatataaaa tatttgtacg ataaaattca tcatataaaa aacacgaatc gtttttgtta
      841 tttttgaaat taatacacta attaaactta aaataataga agtatatcca aacatagctc
      901 caacatttgg acttgtatag acaccaaata accgatgttc aataaaacct tgtcttgcaa
      961 gtaatcctgg cgttcctgtt gggacatgat aagaaatcaa aaaaataaat tgatagatag
     1021 atattagcgc agctaaaaaa attatgataa tgaaaacatt attaaaatac accatttttt
     1081 tctttttttc ctcttcactt tgtgaaaagt ctgcaggaaa aattactatc aaagtaatca
     1141 gcaaatatcc aaaattataa atagatggaa ttaaaaaatt ttctctgtta atgattatag
     1201 ttaagataaa actaatcatt gctccaatca gtagccaaga aaaacgcatt ttaaaaaaat
     1261 ttttctttat aaaaacctca tacaaaacta taattccacc ccaagcaaaa gctattttca
     1321 tcgggatatt agttacatta aaagtactag gcataaaata aagcagacaa atgattaaat
     1381 ttattactcg aaacgcatct cgacttaata aactcttctc taaaacaggt actattctat
     1441 tcatctatat ttccaacttt caaaatttc
//
LOCUS       DAZAFF010000207         1461 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000207 DAZAFF010000000
VERSION     DAZAFF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.218"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..486
                     /locus_tag="ACG9BB_002986"
     CDS             76..486
                     /locus_tag="ACG9BB_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN2838831.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            500..856
                     /locus_tag="ACG9BB_002987"
     CDS             500..856
                     /locus_tag="ACG9BB_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFN2838832.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          540 a          209 c          271 g          441 t
ORIGIN      
        1 attttattag ctaataattg gtattttaaa agaaattact gtagaattgg attataatta
       61 aaaaggagtg agaaaatgat aaaagtatat acttcgccta gctgtacttc ctgtagaaaa
      121 gcaagagcat ggttgcaagc gaatcaactt gaatttgaag agaagaatat ttttgccgaa
      181 ccgttaacag aaagtgagat caaaaagatc ttggcagcga ctgaaggagg agttcaagat
      241 attatttcca ctcggtcgaa aatttacgaa aagctgggca tagattttaa cgaattaact
      301 ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat tattgagacg acctattttg
      361 attgatgaaa aaagaatact aatcggattt aatgaagatg agatccggtc atttgtccct
      421 cgtgaaattc gatgtgttgc cagaaatctg atctatttac aaaacaataa catcttaaat
      481 tcatagggag ggagccttcg tggaagtgaa agtctatgga tcagcgcact gtgtaacaag
      541 tctgaaggcg aaacaatggt tagaacacca tcaattgcct tatcaatttg ttgacttaga
      601 agaacgcgat ttaagtacag aagaagcaga agaattatgc agttttgaag aagtgcaagc
      661 agaacagcta tttgcgactt ggtctgaggc ttttcacaac attgcgattg acctttcctg
      721 tgtaggaaaa aaacaactga tttttttatg cacacaacat accaatctcc tcagacgtcc
      781 tttgattatt atcgatgaag ttctatttat tggatacaat gagcagttga tggaacagct
      841 tatcttaaat aaatagactt taactgtttt aatcaagtca ctgagcattt catacagttg
      901 taacagtcga acacttagca tcaagcgtgt tatgtgttct ttttttgact ttatttttct
      961 actatagaga catgctcaat ttatgtggtc aaaattcaaa tctaggtggg gatatagagt
     1021 taaaaatata ttgacatgtg taaatttaca tgatatattc atttctgttg ttttaagtag
     1081 caaagcaata aaatagtatt ttaaaaaaca tataaaatag ttgttgacat gttagaaatg
     1141 acatgataag ataactgagt tgttaaaaaa gaaccacttg tgaagcttga ttctatcgaa
     1201 aaaaacttca aaaaagttct tgacaaataa caaatgataa gttatgataa atgagttgct
     1261 gtaagatttc gaaaaacgaa ataaaaaaaa cttttaaaaa agagttgaca attacttgtc
     1321 tctctgatat gatataaaag ttgctacggc agccaaaaac gaatcgggga acactcctga
     1381 tgaagtagac ctttgaaaac tgaacaaagt aagacaaacc aaatgtgtag ggcgtcttga
     1441 ttcaattcaa gacaacaaac a
//
LOCUS       DAZAFF010000208         1454 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000208 DAZAFF010000000
VERSION     DAZAFF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.219"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1077
                     /locus_tag="ACG9BB_002988"
     CDS             <1..1077
                     /locus_tag="ACG9BB_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFN2838833.1"
                     /translation="INLLKKEKEDLNDEINQKYDETLKNVYSSLIVSNIEETSSSEIK
                     NKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGLLNKLTISNID
                     STREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKE
                     QMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEE
                     KLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSA
                     SVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENY
                     NGTVTFTKLAKAEQYRRSLELSKD"
     gene            1149..>1454
                     /locus_tag="ACG9BB_002989"
     CDS             1149..>1454
                     /locus_tag="ACG9BB_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838834.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     "
BASE COUNT          644 a          165 c          227 g          418 t
ORIGIN      
        1 attaatttat taaaaaaaga gaaagaagat ttaaatgatg aaataaatca aaagtatgat
       61 gaaacattaa aaaatgtcta ttcttcactt atcgtttcaa atatagaaga aacttcatct
      121 tctgaaataa aaaataagct ttctttgtta aaattaaaag aaaaggaata tattattgac
      181 gatgctgttt ctgtccctct tattttaaat aaacgagaga aaaataattt aaagaaaaaa
      241 atgctcattc catttgattc agaagtgaaa ggattactca ataaactaac tatttctaat
      301 atagactcga ctagggaaaa aatcataaaa atatttgata aaattaataa tttattcaag
      361 aatgacaatg ctgaattaag gaagggtctt ttagagttta aacttcaaga actagaactt
      421 cattactctt atttagtgaa aattaccgaa gaaaaagaac aacaaaaagc aataaaagaa
      481 cagatgatag aagaggaaaa agttcgtaga gaaattgatc gtgaaaagaa acgtattgat
      541 aaagaacaac gtcaattcaa ttctgaaatt tccaaattaa tcgcatatat gcaaaaagct
      601 aatgcagatg tggaaaaaga attatatgca aataaaattc aagaactgga agaaaaatta
      661 aaagaattag aagttatacg tgaaaatgta ttacaaagag aattaaatac tagagccggt
      721 tatgtgtatg taattagtaa tattgggtct tttggtgaag acatatataa aattggtatg
      781 actagaagat tagaacctat ggatagaata aaagaattga gtagtgcatc ggttccattt
      841 gaatttgacg ttcatgcgat gattttttca gaggacgctc cgtctttgga aactaaattg
      901 cataatcatt ttagaaaaca agaagttaat aaaattaatc aaagaaaaga atttttcaaa
      961 gtttcactag acgaaataga gaaagttgtt ttagaaaatt ataatggaac agtaacattt
     1021 acaaaattag ctaaagctga acaatataga cgatctttag aattgtcaaa agattaaata
     1081 tataaaaagc cttcgggctt tttcttttaa caattaatag gaacgtatat tcgaaaggaa
     1141 tagaaataat gtggttagaa aaaataaatg acaaaaaatt taagtacacc gaacgttata
     1201 cggatccctt aactgaaaaa aagagaaaag tgtccgttac tttatctagc aattcacgcc
     1261 aagcttggaa tcaagctaac ttacttttga atgaaaaaat cgcagaaaaa ataaaacgaa
     1321 atgaagaatt accactaact tttggagaat taaaaactaa atgggatgaa aaatacaagc
     1381 caactgtaaa agaaagttcc tatcgaacaa ctcaggttta tctatcatta atttcaaaat
     1441 atataaaaga tgat
//
LOCUS       DAZAFF010000209         1432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000209 DAZAFF010000000
VERSION     DAZAFF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.220"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACG9BB_002990"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACG9BB_002990"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFN2838835.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1131..1406
                     /locus_tag="ACG9BB_002991"
     CDS             1131..1406
                     /locus_tag="ACG9BB_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838836.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT          445 a          284 c          213 g          490 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgctattcta
     1021 ataataaata accactattt gatttatata tatttcagta tccttcttgt tttcatataa
     1081 ccaaaggcgc tatgatttcc atatgaaaaa aataaagtca ggtgaaaaaa atgaaacttg
     1141 aggataaaaa agtcattttg aagaaaatca aacgactacc agaccaaata gatggctata
     1201 cgatctgttc tttcaaaaaa gacaggagta taaaagctta tacgcaaaac acagaaaaag
     1261 gaataatcta tttcctacat gaagaaggtt acgataccca aacctttatg tttactgaaa
     1321 aaaaagaatt tcataaacag atgaaaaaac tgattgaaaa agaatttcca agaagtcata
     1381 aactctatgt gaatcagcaa ttttaacttc taactgttct ttattaatta aa
//
LOCUS       DAZAFF010000210         1360 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000210 DAZAFF010000000
VERSION     DAZAFF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.221"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            53..814
                     /locus_tag="ACG9BB_002992"
     CDS             53..814
                     /locus_tag="ACG9BB_002992"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFN2838837.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(929..>1360)
                     /gene="fabZ"
                     /locus_tag="ACG9BB_002993"
     CDS             complement(929..>1360)
                     /gene="fabZ"
                     /locus_tag="ACG9BB_002993"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFN2838838.1"
                     /translation="VLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTINEDF
                     FQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVPGDV
                     LKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          456 a          259 c          258 g          387 t
ORIGIN      
        1 ctattttgtt atcattaaag ataacaaaat agtaaattgg aaaggaacag ttatgaagaa
       61 aaataaagtt attttaagta ttagtttagc agcagcattg ctgtttgctg ctggttgttc
      121 tgcaaacaac acgacttctt ctagttcgtc tacagaaagt tcaaaaagta catctactgc
      181 ggaaagcacg actgccagta gcgtcgattt gaacgcattg gaattaccac aattgacgaa
      241 agatgtcgcc gaaaatgaag atctggtcga aatggtgacg aataaaggca caatcaaaat
      301 caaattattt cccgaacacg caccaaaagc ggttgaaaac tttatgacac atgcaaaaga
      361 cggttactac aacggactga ctttccatcg cgtcatcaaa gatttcatga tccaaggtgg
      421 cgatcctaaa ggcgatggca ctggagggga aagcatttgg ggagaaggat ttgaaacaga
      481 gatcaacaac cacctttata atataagagg tgcgttatct atggctcgct cacaagatcc
      541 taacagtaac ggcagccagt tctttatcgt tcaaaataca gatgatatgc atgatggtct
      601 tttaaaagac gactatcctc aagcaatcat cgacgcttat aaaaacggcg gttatccttc
      661 actagatggc cattatactg tcttcggcca agtgattgaa ggaatggatg tcgtggatgc
      721 tattgcaaat atcgaaacag atagttcaga caaaccaaaa gaagaagtaa aaatcgataa
      781 aatcaatata ctgcaagaag caaaacaaaa ataaaaaaat aacgtctgaa tcctgcatag
      841 atgacaacgt atgctaggat tcaggcgtct tctttttaca taaaaagcaa gtaagcttcc
      901 ttttgttttg ctatatcagg ttttatagtc aagcattttg gttgacgatg aacgtgaact
      961 cgcactcaca gactttttta tcatctacat atgctgcaga taacgctgta ccgactgatc
     1021 ccttcatttt ggtgatttca aaatgacatt tcagcacatc tcctggaaca actttttgac
     1081 ggaatttggc cttattgatc ccaccgatat aagctctttt tcctagaaac tcttctgatt
     1141 ttaggataag tatcgaacct gcttgtgcca gtgtttctag aatcaacgca cccggcatca
     1201 ctggattgtc aggaaagtga ccttggaaaa aatcttcatt gattgttaca ttttttgtcg
     1261 caacgatctt ttttcctggt tctaaagtat ctacataatc aatataacaa ataggataac
     1321 gattaggaat caattccatt acttcagtag cagataatac
//
LOCUS       DAZAFF010000211         1355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000211 DAZAFF010000000
VERSION     DAZAFF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.222"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACG9BB_002994"
     rRNA            <1..1355
                     /locus_tag="ACG9BB_002994"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAZAFF010000212         1332 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000212 DAZAFF010000000
VERSION     DAZAFF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.223"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1332
                     /locus_tag="ACG9BB_002995"
     CDS             82..>1332
                     /locus_tag="ACG9BB_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFN2838839.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENM"
BASE COUNT          452 a          259 c          254 g          367 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tg
//
LOCUS       DAZAFF010000213         1259 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000213 DAZAFF010000000
VERSION     DAZAFF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.224"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(92..1135)
                     /locus_tag="ACG9BB_002996"
     CDS             complement(92..1135)
                     /locus_tag="ACG9BB_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFN2838840.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          433 a          251 c          237 g          338 t
ORIGIN      
        1 ttgttgttag aaggatatct gcaaaattaa aaacagttgt gattcggaca ttgttccatg
       61 cgagaatcac aactgttttt tcatgttcat tctaaacttt gatcagccaa ataaatagta
      121 aagccatcac tgtttggaga agcccgacag ctaatccata agataagaag cgtgtacctt
      181 ctttcatgaa ttttgcaaag tctaaacgca gaccaattgc cgctaaagca gtgatctcaa
      241 accagctgct gataaaatgt gccgattggt caataactac aggtaaagaa aacaagctgt
      301 tgaccaaaca aaagatcaca aaccctaata cataccaagg aagcggtaat tttcgttttt
      361 tctgcgtatc agttggcgtt tcaaacagag ctggttgttt cttgtgcatg aatttttcaa
      421 acagatagac aacaacaacg agcatcataa tacgtagaat cttaaatagc atggcgaatt
      481 cgacaacaga attgtcaagt aagcttgccc cagcgactac ttgaccaaca gattggagtg
      541 taccacccaa aagtgcactt ttttcaatga gatttccacc aaacaactgt aatccaagaa
      601 aagggagagt caacatcatc acagtaccca gtagattgac tagtgtgatg atctgtcctt
      661 tttcttcctc tttcgcatgg atggaagggg cgattgaagc aatagcagaa gaaccacaaa
      721 cagcgtttcc tccagccatc atcaaagaag aagtatcgct gaattgcagt tttttaccaa
      781 tcaagtaagc accgatgata gtgactgaca tttgcagtaa cacaaaaaca attcctttta
      841 caccaatttg agcaatcgtt tgagctgtga cagttgcacc tagtaaaaaa acagagaatt
      901 ctaatagttt gctttcagca aattttgtgc cgattgctag aacaggctgt ttaaaaaaag
      961 tatttcctaa taaaataccc aataaaatag caatagtcgc tccacctaac tgtggcagat
     1021 aaatagcaag aagcctgctg aaggcagcga tggcaaaaga aagcagtaaa ccgggtaaga
     1081 tttcgcggac cgctgaatcc tttacagatt ttattggatg tgacaatgat ttcatttgat
     1141 cggttccttt cgtcaataaa tactaaaagc atactcgaac atagtagcac taaaccagta
     1201 ttttgacaaa agaacaagag gtcgtgacaa aagtcttgta cgacctcttg tttcgaata
//
LOCUS       DAZAFF010000214         1246 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000214 DAZAFF010000000
VERSION     DAZAFF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.225"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG9BB_002997"
     CDS             200..742
                     /locus_tag="ACG9BB_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838841.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACG9BB_002998"
     CDS             898..>1246
                     /locus_tag="ACG9BB_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFN2838842.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAZAFF010000215         1215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000215 DAZAFF010000000
VERSION     DAZAFF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.226"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACG9BB_002999"
     CDS             414..947
                     /locus_tag="ACG9BB_002999"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFN2838843.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAZAFF010000216         1212 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000216 DAZAFF010000000
VERSION     DAZAFF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.227"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..409)
                     /locus_tag="ACG9BB_003000"
     CDS             complement(38..409)
                     /locus_tag="ACG9BB_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838844.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(411..812)
                     /locus_tag="ACG9BB_003001"
     CDS             complement(411..812)
                     /locus_tag="ACG9BB_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838845.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(826..>1212)
                     /locus_tag="ACG9BB_003002"
     CDS             complement(826..>1212)
                     /locus_tag="ACG9BB_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838846.1"
                     /translation="IISEDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVS
                     EIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHY
                     AVVKFNGTEIGRQIFDIKVNKPVEKN"
BASE COUNT          385 a          218 c          154 g          455 t
ORIGIN      
        1 aaataaaaaa ataacaaaca aactcgcttg aaattcatta ggtgagtttg tttattattt
       61 tttctgcttc tcgattcatc agcgccatat ctgttttcgt aatggtttca ttaggaatat
      121 agtcagcaca ttttcttttc tctaacattc tatacccact attatgtatt cctaaggtat
      181 tcaattcctt aactgttgaa aacaaattcc aaacggtttg gtggcttcct tttccgctac
      241 ttccattata aataatatca taattttctt tcaaatactc atcagatttt ttaaaagcac
      301 tgtagtatgc tcttgaaaca gcagttcttc tataagattc tttagaatta tatttatcat
      361 ctgattcaat ttgtttggaa atagataaat attcattaaa gtcaaacatt ttattccccc
      421 aaagttgcta ctacagattt ccccatatat ttatagtcag ataatacatt atcgagagta
      481 tccaaaacat cgtaatctga ggaaaacaat tcagatgttg ttttgataac aaagaaatac
      541 aagtttaaat cttcatcata ttttatattg atttcagctt ttctttttaa aaccgtattt
      601 atcacatcaa aaattttgcc atcattactt gaaattgtgt taatctcatt ttttgtagta
      661 attacaagtt gattatcatt tttagttttc ggagtagaac ttgcatccac aacaactgga
      721 gatgctagaa ttaccgatgc gcaagcaaaa atcgcagcaa aatttattaa gctaccactc
      781 cgatgtgtat ttgatggtac tatatcaagc atccgtatcg tctccttaat ttttttccac
      841 tggtttgttg accttgatat caaagatttg ccttccaatc tcagttccat taaatttaac
      901 aacagcataa tgctctcctg cttcttcaac caagacattt ctggcgttta tattaaagtt
      961 tatatcgtta acttgaggag gcaagcttac cgatacttca cccgtagaaa aaatttgttt
     1021 ttcattcttg tttaaaactt caatttctac tatttttatc ttttctgtat cctctatctc
     1081 agacaccaca gctgttacag aaaatgataa agcagtagga attaaaggcg cattaataga
     1141 aagagctgga ttaattactt tattaataac attagtgcca aattgctgac tttctacatc
     1201 ttctgaaatt at
//
LOCUS       DAZAFF010000217         1200 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000217 DAZAFF010000000
VERSION     DAZAFF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.228"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG9BB_003003"
     CDS             <1..969
                     /locus_tag="ACG9BB_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFN2838847.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          403 a          216 c          242 g          339 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
//
LOCUS       DAZAFF010000218         1195 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000218 DAZAFF010000000
VERSION     DAZAFF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.229"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG9BB_003004"
     CDS             <1..1076
                     /locus_tag="ACG9BB_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN2838848.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAZAFF010000219         1187 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000219 DAZAFF010000000
VERSION     DAZAFF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.230"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACG9BB_003005"
     CDS             <1..186
                     /locus_tag="ACG9BB_003005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838849.1"
                     /translation="SVLMSNYKRTKVKNYIYLLAYFFFIIVCLIFINIRGTAFVNGIL
                     PLFRENIIFKAVYYFFE"
     gene            223..1161
                     /locus_tag="ACG9BB_003006"
     CDS             223..1161
                     /locus_tag="ACG9BB_003006"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFN2838850.1"
                     /translation="MGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLN
                     QMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCIT
                     GNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPN
                     ELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKG
                     NKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQ
                     LAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
BASE COUNT          447 a          142 c          215 g          383 t
ORIGIN      
        1 agtgtattaa tgagtaatta taagagaaca aaagtaaaga attatatcta cttgcttgcg
       61 tatttctttt ttatcattgt gtgtttgata ttcatcaata taagaggaac tgcttttgta
      121 aatggaatcc taccattatt tagagaaaat attattttta aggctgttta ctattttttt
      181 gagtaagaat aaatagcact taaaaataga gatgagggat ttatgggaag acaagaatta
      241 tatagtttag ctatcaatga attggaaaaa agaaatcttg aaatacatga attaaatcaa
      301 aaaaataatt ctattttagg catgaaaata cgaaataaaa ttaatcaact taaccaaatg
      361 aaaaaacgag ataatctgtt gccagcaaaa tataccctta aatcgatgac cggtaagggg
      421 cctattaaag actattcaaa cagagagaat cgcagtgatg aactaagaaa aaaagagtta
      481 actaatcaag gaacacctaa aaggattgct gtgtatagtt gtatcactgg taattatgat
      541 tctattaaag aaccggattt ttctcatcca ttggtagatt actatttgta tacagataat
      601 gtgattacaa gtgagcattg gaatattaga gagataccta aatatatatc ggaattaaaa
      661 aataatattt tgatcaatag atatattaaa tttcatccta atgagctatt tactgactac
      721 gattttactt tgtatgttga tggtaatgta acaataactt ctgatattac cggattactg
      781 aacagttctt ccaaacttgg gttaagtatg cataaacata gtgaaagaaa atctttatat
      841 tctgaggcag atgcattaat tgcagttggc aaaggtaata aggaaagtat tatagagcaa
      901 attagtaagt acagagaaga aggatttcct gataattttg gattggctga agccacggtt
      961 attttagttg atttaaataa ttcaatcgct gagaatcttc tagatttgtg gtggagagag
     1021 tatttgaact cagaaagtgg gcgagatcag ctcgcatttc cttacgtgtt gtggaagtcc
     1081 ggacatgcaa tgaaggaagt gactacctta ggcaataatg tatataaaag cgctaaatta
     1141 agaatcataa gtcattatta gccctaaatg atttctaaac tattctt
//
LOCUS       DAZAFF010000220         1064 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000220 DAZAFF010000000
VERSION     DAZAFF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.231"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..993)
                     /locus_tag="ACG9BB_003007"
     CDS             complement(37..993)
                     /locus_tag="ACG9BB_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838851.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          178 c          160 g          380 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aact
//
LOCUS       DAZAFF010000221         1062 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000221 DAZAFF010000000
VERSION     DAZAFF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.232"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACG9BB_003008"
     CDS             <1..963
                     /locus_tag="ACG9BB_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFN2838852.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          366 a          195 c          213 g          288 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc ca
//
LOCUS       DAZAFF010000222         1040 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000222 DAZAFF010000000
VERSION     DAZAFF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.233"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..700
                     /locus_tag="ACG9BB_003009"
     CDS             <1..700
                     /locus_tag="ACG9BB_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838853.1"
                     /translation="GTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYFVYCPELLL
                     YDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFA
                     KVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTI
                     SFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPE
                     NWAELRKLVEYGII"
     gene            687..>1040
                     /locus_tag="ACG9BB_003010"
     CDS             687..>1040
                     /locus_tag="ACG9BB_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN2838854.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMN"
BASE COUNT          375 a          133 c          152 g          380 t
ORIGIN      
        1 gggtacttat ttgaagaaga gtatattttc aacaagtaaa aacgttcaaa gagtttttaa
       61 aatattaaat aaaaatagca tcttcaaaaa aaaaattgac gattattttg tctattgtcc
      121 ggaattgtta ctttatgatg taaaaaatat aaaaaaaatt cccaaaatag atgaaaacag
      181 taaattgatg aaaaacatac aatcaatctt tgggatagaa gaagtttttg tcggagataa
      241 atgtatcttt tttgatcaac ctttattatc tgatggttat ggcatagatg aaacagatat
      301 tttcgctaaa gtaaatagta tattttctgg aaaaaaagaa actttaatta agcttcatcc
      361 tagagcaagg acaaatcgtt atggtgaaaa aaacatattg aattccagtt taccgtttga
      421 agtcttgcta cttgaaaatg acttctcttc aacacttttg attagtccta tatctacaat
      481 ttctttttca ccatctatga tgtttgaaaa gacgatcaac tctattatgt tggctaaatt
      541 aattttaaat gaatatacag cattatctga agataaaaaa gaaacattga caaatattgt
      601 tcttttctgt gaacattata accgtctatc agaagaaaaa attttactcc cagaaaactg
      661 ggcagaatta agaaagttgg tagagtatgg gattatttaa cacactttcc aagtcaaaat
      721 tagttaagtc cagtttctgg tatactatag ggaatttttt tattaaggga atttcattca
      781 taactattcc tatttttgta aatattacta ctcaagcaga atatggtttg attaataatt
      841 tcacttcatt tgtatcaatt ttttcgattc ttgttgggct aagcctaaat tcggcaatta
      901 ataatgcaag ttttgactat aaagatgaca taaatgaatt tatgtcatcg gtactttctc
      961 tagctacact ttcgttaatt gtgttcttat taatagggaa catatatttt ttgttttcaa
     1021 atgctttttt tgatatgaac
//
LOCUS       DAZAFF010000223         1001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000223 DAZAFF010000000
VERSION     DAZAFF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.234"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1001
                     /locus_tag="ACG9BB_003011"
                     /pseudo
     CDS             370..1001
                     /locus_tag="ACG9BB_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta c
//
LOCUS       DAZAFF010000224          994 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000224 DAZAFF010000000
VERSION     DAZAFF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.235"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(522..821)
                     /locus_tag="ACG9BB_003012"
     CDS             complement(522..821)
                     /locus_tag="ACG9BB_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN2838855.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          337 a          173 c           86 g          398 t
ORIGIN      
        1 taaaataata tttctacact ttttcatttt ttctatctcc cctattttaa ctaaaaataa
       61 aacctactat atcatctctc aaaaatcctt tcttcaattc ctcccccact catttccaat
      121 cttttaacta aaaaattgat agttatttta caaatattta taaaaaataa atatgccaaa
      181 aaactgtttt tacaaggttt ttcctaatgt aaattatttt ttcgcatata ttcattttat
      241 tttacttatt gctttcattt tttcaatcta tatttccaat ttttaactta caaaaatgca
      301 acattaataa ataacgatag tttcataaaa tacactttta attaacatta ttagtttata
      361 ctagctgttt ttgtgttaat taaaggataa aatacaatat aaagaaacat ctgtttcttt
      421 atttagaata aaaaaataca gactctgttc aaataaatga acctagcctg cattatgaga
      481 ttgactatac tttttaattc attgagatga ataactcgtt tttataaatg cccaaaagta
      541 atcggtgccg aagccattcc tcgacctaat tctttttcaa attttcctat tgttgatatc
      601 tccatataaa acggtgatac tggtttagac aattctcccc tgattgctaa cggacgtaaa
      661 ctacgttcca aattatttat gactttagga taatactgac ctttttctaa gcctgtcttt
      721 gcgtctatca aaagtatacg ttccttttct ttgatctctg gatttaaaac gagatcatat
      781 aattttagta ccatgttttc tatttcttca cttttagaca tcacgatttc ctcccaaaaa
      841 tatatttctt cccactacat tctataaaca aagtatacgc tacatctaga tttttttaaa
      901 tcattactgg attgttaaaa cctttcaata cactgttatg gcaacgattt ttattttaaa
      961 aacattctaa attaaatcag taaaaatttt tatt
//
LOCUS       DAZAFF010000225          986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000225 DAZAFF010000000
VERSION     DAZAFF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.236"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>986
                     /locus_tag="ACG9BB_003013"
     CDS             <1..>986
                     /locus_tag="ACG9BB_003013"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFN2838856.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DAZAFF010000226          943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000226 DAZAFF010000000
VERSION     DAZAFF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.237"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACG9BB_003014"
     rRNA            <1..>943
                     /locus_tag="ACG9BB_003014"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZAFF010000227          939 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000227 DAZAFF010000000
VERSION     DAZAFF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.238"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..865)
                     /locus_tag="ACG9BB_003015"
     CDS             complement(<1..865)
                     /locus_tag="ACG9BB_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2838857.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKK"
BASE COUNT          326 a          110 c          159 g          344 t
ORIGIN      
        1 attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata tctgcaattt
       61 ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt tttagttctt
      121 taaaatagct aataacactt tttccattca cttttctaaa atgttttttt aagctggctg
      181 gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa agatgttctt
      241 ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc ttttttttag
      301 gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga taatgccttg
      361 gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa agcaaatgaa
      421 tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag gaaatagctg
      481 gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa gtaatgatag
      541 atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat aattgctcat
      601 caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa aagatttgga
      661 cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata gatttatgtg
      721 caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag ggagcagcag
      781 ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga ttggaggatt
      841 ggttgtttgt taacaaaaga accactttcc tttctctttt tatataaaaa gctttctatt
      901 tataattaat gactattata acaaaaatga aaagccaaa
//
LOCUS       DAZAFF010000228          937 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000228 DAZAFF010000000
VERSION     DAZAFF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.239"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>937
                     /locus_tag="ACG9BB_003016"
     CDS             <1..>937
                     /locus_tag="ACG9BB_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFN2838858.1"
                     /translation="ASFVISYIQSYFTINFNHFKYLILSFISTFLNIAISVALMQTLY
                     FDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYALKISLPLIPHSLANI
                     VLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKK
                     VKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLP
                     VNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTTVFTYFLLFIFHYNI
                     AKKITNKQLFDTKKICIIIIFVSIFSLILHILINQ"
BASE COUNT          300 a          117 c          148 g          372 t
ORIGIN      
        1 ttgctagttt tgtaattagt tatatccagt cttattttac gattaacttt aaccatttta
       61 aatatttgat tttatctttt atatctactt ttttgaatat tgcaatctct gtagctttga
      121 tgcagacatt atattttgat gataaatatg ttggcagagt agtcggcgga acactgtcat
      181 ttgtgttgat aggtttagta atttatttaa gaataatgat aaagggtgct catttttttg
      241 ataaaaaaca gtggagatac gctttaaaaa tctctttgcc gctaatccca cattccttag
      301 caaatatagt tttagcacaa ttcgatagaa ttatgattaa ttcttattta ggtcctgctg
      361 cggggggaat atattcctac attagtaaca taggtattat tctaagcgta atatggatgt
      421 ctactaataa cgcctgggtt ccatggttct atggtgagat gagtaaaaat aatgaaaaaa
      481 aagtgaagaa agttagtaac tattatttag tagtctttac tttgattacg atgattgtaa
      541 tggtagtttc gattgattta gcaaaattta tggctccgaa agactattat tctgggttat
      601 ctttagtggt tccgataacc ttaggctatt tctttcagtt tttatatagt ttaccagtta
      661 atgctgaatt ttatgagaaa aaaacatcgt ttattgcgat tggtacagta gcaagtgcta
      721 tagttaatat actattaaac atgattttca tacctaggtt aggaataatt gctgctggat
      781 acacaaccgt atttacgtat tttttacttt ttatttttca ttataatatc gctaaaaaaa
      841 ttacgaataa acaattattt gacacaaaaa aaatatgtat cataataatt tttgtatcaa
      901 ttttttcttt gatacttcat attttaatca atcaaat
//
LOCUS       DAZAFF010000229          906 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000229 DAZAFF010000000
VERSION     DAZAFF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.240"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..767
                     /locus_tag="ACG9BB_003017"
     CDS             <1..767
                     /locus_tag="ACG9BB_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFN2838859.1"
                     /translation="IKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKYYISLLCKEE
                     IIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKN
                     KGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGML
                     RNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLE
                     IREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          335 a          144 c          164 g          263 t
ORIGIN      
        1 tcatcaaagt tcctaaattg aagtcattag taagaattaa gcttcataga caaccaaaag
       61 ggattatcaa atctgctaca atatcacgtc attctagcgg taaatactat atttctttgt
      121 tgtgtaaaga agaaattatc gaattaccta aaactaattc cacaattggt attgacttag
      181 gtattatgga ctttgcgatt ctttctgatg gacaaaaaat tgataataat aaattcacgt
      241 ccaaaatgga aaagaaacta aagcgtgaac aacgcaaatt gtctaggcgt gctttattgg
      301 ctaaaaataa aggtaccaat ctatttgaag ctaaaaatta tcaaaaacaa aaacgcaaag
      361 tagctagatt gcatgaaaaa gtaatgaatc aacgtactga ttttctgaat aagttgagta
      421 cagaaatgat caaaaatcac gatattatct gtattgaaga cttaaacaca aaaggtatgt
      481 tgcgaaatca taaattagct aaaagtattt ctgatgtttc ttggtcaaag tttgtgacta
      541 aattacaata caaggctgac tggtatggac gaaaaatcat caaagtagat aaatggtttc
      601 cgtctagtca gatttgttca gagtgcggac ataaagatgg caagaaatct cttgaaattc
      661 gagaatggac ttgtcctgtt tgtcatactc atcatgatcg tgatatcaat gctagtatca
      721 atattctgac cgaaggtcta agactacatt ctatgggatt ggcttagatc caatcatcaa
      781 gaaccgtagg aactacgggg atagcttggt aaataagaga caccgctgtt agtaaagaaa
      841 tacgctatca agtatgctct attcccaaga agctcccact tcaagcgtta agaaccgtca
      901 ggttta
//
LOCUS       DAZAFF010000230          891 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000230 DAZAFF010000000
VERSION     DAZAFF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.241"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..>891
                     /gene="bsh"
                     /locus_tag="ACG9BB_003018"
     CDS             6..>891
                     /gene="bsh"
                     /locus_tag="ACG9BB_003018"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFN2838860.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQIT"
BASE COUNT          304 a          122 c          161 g          304 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact g
//
LOCUS       DAZAFF010000231          890 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000231 DAZAFF010000000
VERSION     DAZAFF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.242"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACG9BB_003019"
     CDS             <1..888
                     /locus_tag="ACG9BB_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFN2838861.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAZAFF010000232          857 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000232 DAZAFF010000000
VERSION     DAZAFF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.243"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..>857
                     /locus_tag="ACG9BB_003020"
     CDS             74..>857
                     /locus_tag="ACG9BB_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFN2838862.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKV"
BASE COUNT          302 a          129 c          154 g          272 t
ORIGIN      
        1 taaatagaaa aaaggaagtc ccttctgtag aatagagtta ccacaacaca ttcacagaaa
       61 agaggacttc catatgaacg attttactac agaaattatg gaaatactaa tcaataaagg
      121 tgatttggat gatttgtttc gtcgtcacct agaattggct attaacgctt tattacaagc
      181 tgaattaacg gtatttcttg actatgaaaa gtatgataga gctggattta attcaggtaa
      241 ttcccgtaat gggaattatt cgcgctcttt taaaacagag tacggtgaat tgaatttagt
      301 aatacctaga gataggaatg gagacttttc tcaacaaact ttacctgctt ataaaagaac
      361 caatgactct ttagaaacca ctattattca gctagttcaa aaaggaatta ctatgtccga
      421 aatctctgat ctaatcgaaa aaatgtatgg tcattattac aaaccacaaa ctatttcaaa
      481 tatgagtaaa attgtgtctg aagatgtttc ggcatttaaa gaaagaactt tagaagctaa
      541 atactcagtc atttttatgg atgctactca tattccctta aaaagacaaa ccgtatcaaa
      601 agaagctgtc tatatagtaa ttggtatcag gttggatgga actaaagagg ttctaggatt
      661 tactatagct ccaactgaat ctgcttatgt ttggaaagag attcttcaag atctaaaaga
      721 ccgtggttta gaagaggttt tgttagtcgt aacagatggt ttaagtggta ttgacgatag
      781 tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata tatctagaaa
      841 tatcgctcat aaggttc
//
LOCUS       DAZAFF010000233          843 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000233 DAZAFF010000000
VERSION     DAZAFF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.244"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>843)
                     /locus_tag="ACG9BB_003021"
     rRNA            complement(<1..>843)
                     /locus_tag="ACG9BB_003021"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          184 a          259 c          187 g          213 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gct
//
LOCUS       DAZAFF010000234          831 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000234 DAZAFF010000000
VERSION     DAZAFF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.245"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>831
                     /locus_tag="ACG9BB_003022"
     CDS             <1..>831
                     /locus_tag="ACG9BB_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838863.1"
                     /translation="YLFPAFKNMEIFGFYDTAFHINRALSLESIFSSPINFETFRSYG
                     MQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSS
                     FFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTL
                     LIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIE
                     QTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLLICLIILTFNFKKL
                     G"
BASE COUNT          263 a          115 c          120 g          333 t
ORIGIN      
        1 atatttattt ccagcattta aaaacatgga aatatttggc ttttatgata cggcatttca
       61 cattaatcgt gcgttaagtt tagaatctat ttttagctcc cctattaatt ttgaaacgtt
      121 tagatcatat ggcatgcaag taaataattt ttacccatgg ttaactctat atcctttatt
      181 tttattgatc aagtttacta atttagcaat aggctataac ctttttcttt atattgttac
      241 gctcattact ctatttatat gtcattatgt aatgtatgaa attaccaaaa aacatgttac
      301 tagctccttt ttcgctatta tatatacaac atcttctttt aggagtgtag aaatatttct
      361 tcgaggggca atgggggaat tactcgcaat gtcaatactt cctcttattt tactcgggtt
      421 tataaagctt tacgactcaa aaaaagaatc ttgggttatg ttggcgatta gcatgacttt
      481 attaatttac actcatgttc tttctgtagc tatgactatg gtaatgatta taattgcttt
      541 aattattcaa ttttatcgga aaaagaaaat agaaatcttc ttaattatta aattaataaa
      601 agctgctata gttactttac ttctttcact agcgtttata ggtcctatga ttgagcagac
      661 attgtatcaa gatctgaata gaccttatgt ggatatatta caaaaaagag caatttatct
      721 gggtaaagag tttttgattg gaagtataga ttcagaatta ctatcatttg gtattggctt
      781 aactttgctt atttgtttaa ttatactaac atttaacttt aaaaaattag g
//
LOCUS       DAZAFF010000235          731 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000235 DAZAFF010000000
VERSION     DAZAFF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.246"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..569
                     /locus_tag="ACG9BB_003023"
     CDS             <1..569
                     /locus_tag="ACG9BB_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN2838864.1"
                     /translation="NNLTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQK
                     VASLENDKNQLVSEKTNLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWN
                     NRLQNEKNKFNEEQSTINSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNY
                     IEKLQKAKNDVEDTANKAQQIVNEHTIQ"
BASE COUNT          335 a           98 c           98 g          200 t
ORIGIN      
        1 acaacaattt aactatatta gacacagctc tagaaaataa agagcaaaaa attagtattt
       61 taaattcaca attgtcttca aacaaccaaa ctcttgaaca actaagaact aatattgaag
      121 tttccaaaca aaaagtggca tctttagaaa acgacaagaa tcaattagta tcagagaaaa
      181 ctaatttaga aaatcaattg tcagcaaaag actctgaatt gcaaggaaaa caaaatgaaa
      241 ttaatgcgaa aatagacgaa attaaccaaa aagtagctga aataaatcga ttaaatagcg
      301 attggaataa tcgtttacaa aatgaaaaga acaaatttaa tgaagaacaa tctactatta
      361 atagtttaaa ccagcaaatt aatcaaatgt tgagtgaaaa aaataacatt caaaatcaac
      421 tgcaagctac tcagcaaaag ctagaagaag aaaagtcaaa aaatagtagt ttgaataatt
      481 acattgaaaa gcttcaaaaa gctaaaaatg atgtagagga taccgcaaat aaagcacaac
      541 aaattgtaaa tgaacataca atccaataga tatagtttta atttaacaaa tggtatgttt
      601 ggagataatg agatagaata taaatgtcat aatattatac aatctaaaca ccgtttcctt
      661 ttctattttt gatgacatca gtatatagct actcgaaaga gtagtctttt tattattctc
      721 gcttattatt g
//
LOCUS       DAZAFF010000236          697 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000236 DAZAFF010000000
VERSION     DAZAFF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.247"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..629)
                     /locus_tag="ACG9BB_003024"
     CDS             complement(54..629)
                     /locus_tag="ACG9BB_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFN2838865.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          125 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaacg
//
LOCUS       DAZAFF010000237          676 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000237 DAZAFF010000000
VERSION     DAZAFF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.248"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..447)
                     /locus_tag="ACG9BB_003025"
     CDS             complement(<1..447)
                     /locus_tag="ACG9BB_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFN2838866.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT          201 a          135 c          103 g          237 t
ORIGIN      
        1 agacacttgc ttaaagcgaa aaatttcttc aatgtccatg cctaattctt tggatattct
       61 cttgtctgac catccatttt ttactaatct ttggatgaca atttccattc ctcttatttc
      121 atgggtccct cttgcacgat tgtgtcgaat tgttgaagcc atacgttctt ctatgggttt
      181 atcaataact gttaccggta caacttttaa gttgagtctt tccttagcaa cacgatacct
      241 atgaagcaca tcaaccaaga tatacttctc ttctttttcc ttgtaataac aaactaatgg
      301 ttgagtaaag ccatcttcaa tgatagaaag ctccaataag ttcatttccg gagacgctac
      361 tcgattggga ttataatcgt tcgcctgtat tttggtaatt ggaacaagcc gaacgtccat
      421 cacagggaaa gttatgtctt tattcatacg cttgtatacc ctactttctt aaaaagtagg
      481 gtatactcaa gaatccgatt aattatcttc tcaatttgga taatatatct catcagtctt
      541 cttgtcactt tatcaacatg attttgctgc aaaatgggat accgacagta ctggactggt
      601 aatcaggata aaagcaaaaa taattaattt gtatcctaaa aaatccataa aaattctctc
      661 acattttcgg gtatta
//
LOCUS       DAZAFF010000238          666 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000238 DAZAFF010000000
VERSION     DAZAFF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.249"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          232 a          108 c          100 g          226 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcatga ctgacgtgcc ggacgcacgt agcgatcatc ggcgttatga
      121 ctatataaag tatactttcg ttgatccaga aaggtataaa gattaaagta ggctacctaa
      181 aaaggtagcc cggaacgaat tttatcacca tacttgtgaa aggagatatt ttttaagtta
      241 gtattaagat tgtgtaatat gatgatatct atattttata gtatcagtgc tataaaatca
      301 aaaataagtc actaattaac taccactcca attgtaagcc ttttttctca ctttttttca
      361 aaaatatggt atgcttttta atggcttcaa atataaaaga gtttaaagcg taacacactt
      421 atggggaagt ggtttggggt gcgctttaaa ctcttcttta ttattatctc acaatttaac
      481 ccaaatgtct ttctatttaa aaatcaaagt aaaacttttc aaatatacag aagtataact
      541 atgtgaaaca tcctttcatt aatccataaa aggatacata aaaaagccac tcatttgagt
      601 ggcaatgaag aaaagcttta gcttgtataa tactcttcaa aaaaattcta acacagaacg
      661 attcaa
//
LOCUS       DAZAFF010000239          648 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000239 DAZAFF010000000
VERSION     DAZAFF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.250"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..515)
                     /locus_tag="ACG9BB_003026"
     CDS             complement(<1..515)
                     /locus_tag="ACG9BB_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFN2838867.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNID"
BASE COUNT          226 a          104 c           92 g          226 t
ORIGIN      
        1 aaatcgatat tttttaaatc agtatcaaaa aagctagcat tttctaaatc attagtagaa
       61 aacattattt tagtgaggga acagtctaca aaatctgcgc ttgataagac agagtgctca
      121 aaggtacttt cttttatgtt tgaaccagat aaatttacat agtccatttt acaattctcg
      181 aataacgtgt tcccaatata actttccatc aatttagtac ctgttaattt acaattgaca
      241 aatttcacgc gatgcaacat gctttttgtc aatatacagc ctgaaaaatc acagttttcg
      301 aatatacagt cggtaatctc cattctttga aaatcattgg aagcaaaaaa tgcatacttg
      361 aaaatagatt tgtaaataat tggatggcag attatttggt tttcgatgac ctctttttgt
      421 gaaaggatat tatacagttc aaaatcttcg ttttcataga tatgaaaaaa ggatccttta
      481 ttcaagcttg cttcattaac tttaggaaaa atcatattta attacctcca ttttcataaa
      541 gtcatattaa caaaagctaa accaacttac attagcagaa aatgagatag gttttcgcct
      601 ttttcgataa cgatagtgtc gctatctctt gaaacgttct tagaattg
//
LOCUS       DAZAFF010000240          625 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000240 DAZAFF010000000
VERSION     DAZAFF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.251"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..625
                     /locus_tag="ACG9BB_003027"
                     /pseudo
     CDS             1..625
                     /locus_tag="ACG9BB_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          247 a          105 c          118 g          155 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataa
//
LOCUS       DAZAFF010000241          583 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000241 DAZAFF010000000
VERSION     DAZAFF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.253"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..440)
                     /locus_tag="ACG9BB_003028"
     CDS             complement(<1..440)
                     /locus_tag="ACG9BB_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFN2838868.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYEL"
BASE COUNT          165 a          105 c           72 g          241 t
ORIGIN      
        1 agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa tgtttcatcc
       61 atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc tttgattcct
      121 aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata aaaataaatt
      181 gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca ttccgaatgt
      241 ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct ttctcttgca
      301 atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg ttgtgcagcg
      361 attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa actaggaacg
      421 aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt taatttattg
      481 ttctactaaa ttcatctttg ttttacaaga acattatatt tctgttagtt tgttttgtca
      541 acgctttcaa aagaaattta ttttgttaaa tttgatctac ttg
//
LOCUS       DAZAFF010000242          534 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000242 DAZAFF010000000
VERSION     DAZAFF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.254"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACG9BB_003029"
     CDS             <1..>534
                     /locus_tag="ACG9BB_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFN2838869.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAZAFF010000243          509 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000243 DAZAFF010000000
VERSION     DAZAFF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.255"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG9BB_003030"
     CDS             complement(<1..132)
                     /locus_tag="ACG9BB_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFN2838870.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DAZAFF010000244          481 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000244 DAZAFF010000000
VERSION     DAZAFF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.257"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>481
                     /locus_tag="ACG9BB_003031"
     CDS             <1..>481
                     /locus_tag="ACG9BB_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFN2838871.1"
                     /translation="TFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKW
                     PEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDL
                     PKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG
                     "
BASE COUNT          194 a           74 c          112 g          101 t
ORIGIN      
        1 taaccttcac cgcaggagaa ggaacaagta ttgaaagtgg aacaacagtc tttgcagtga
       61 aagacggcgt aagcttacca gaagataaac ttccagtgtt gaaagcaaaa gatggttata
      121 cagatgcgaa atggccagag gaagcaactc aaccaattac agcagatgat acagaatttg
      181 tatcaagtgc aacaaaactg gatgataaat ctgacgctga caaatataat cctgaaggtc
      241 aaaaagtgac tacagaattg aataaggaac cagacgcatt tgagggaatt aagaacaaag
      301 aagatttacc aaaagatact aagtatactt ggaaagaaaa agtagatgtt agcgcagctg
      361 gaaataaaaa agggactgtt gtagtgacat atccagatgg atcatctgat gaagttgaag
      421 tagatgttac agtgacagac aatcgttctg acgccgataa atatgagcca acagtagaag
      481 g
//
LOCUS       DAZAFF010000245          469 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000245 DAZAFF010000000
VERSION     DAZAFF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.258"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          165 a           57 c           66 g          181 t
ORIGIN      
        1 aaaacaacaa attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga
       61 tataatacaa aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc
      121 ttttttgtaa tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc
      181 aacgatagtg tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt
      241 tttacataac ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt
      301 ttaaagcgca ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt
      361 tgaattcact aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt
      421 aggatttaga gtaattaatt agatgtttat tttataggac tgatactat
//
LOCUS       DAZAFF010000246          468 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000246 DAZAFF010000000
VERSION     DAZAFF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.259"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACG9BB_003032"
     CDS             <1..>468
                     /locus_tag="ACG9BB_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFN2838872.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAZAFF010000247          462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000247 DAZAFF010000000
VERSION     DAZAFF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.260"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACG9BB_003033"
     CDS             complement(<1..430)
                     /locus_tag="ACG9BB_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN2838873.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAZAFF010000248          459 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000248 DAZAFF010000000
VERSION     DAZAFF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.261"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..234
                     /locus_tag="ACG9BB_003034"
     CDS             <1..234
                     /locus_tag="ACG9BB_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFN2838874.1"
                     /translation="DLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQ
                     AVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT          169 a           85 c           76 g          129 t
ORIGIN      
        1 gatttgattg atagagatta ctttactcag gcagacttga tcattacact ttgtggtgat
       61 gccaaagaca agtgccctgc cgttccaaac aatcacgaac accttcactg ggacctaaaa
      121 gatcctgcac aagctgtcgg gagcgaagaa gaaatattaa cggaattcag aaaagtcaga
      181 gaaggaatca aaagaaaagt tttagactta aatcaacgta catcgatcgt ataaagaagt
      241 attcaaaatg atttttcaag cactaagaaa caatgttaaa aaaatagctt tccatatttt
      301 acaaaattgt gccttattgc cgacaaattg tctcttgaac accgtttacc agtagtcaag
      361 acatcgtaag caactataaa aaattataag aaactcaaaa taaaaggatg tgccacaact
      421 ttggccacat cctctttttt tgtatttagt tttctactt
//
LOCUS       DAZAFF010000249          451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000249 DAZAFF010000000
VERSION     DAZAFF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.262"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>451
                     /locus_tag="ACG9BB_003035"
     CDS             <1..>451
                     /locus_tag="ACG9BB_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN2838875.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DAZAFF010000250          450 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000250 DAZAFF010000000
VERSION     DAZAFF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.263"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(212..334)
                     /locus_tag="ACG9BB_003036"
     CDS             complement(212..334)
                     /locus_tag="ACG9BB_003036"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838876.1"
                     /translation="MNMNRDVLKMNEFDQSLSYRNLQFRSIGLSINEKVKKNMF"
BASE COUNT          143 a           87 c           52 g          168 t
ORIGIN      
        1 ataaaaaaat tattacgtta ttaaaaaaac agatgaaaga tcaggtttta ttagatctca
       61 ttctttcatc acttgaaggg atttaatgaa ctactttttc ctctttgtcg tttttatata
      121 ttcgtattcc ttcaaacagt aggatagcct ctttcaatgc ttgatatttg aactgtccgt
      181 tacttaaaaa tttagatgtg ccgccctctt cctagaacat attctttttt actttttcat
      241 taattgacag accaatcgaa cgaaactgta aatttcgata ggataatgat tggtcaaact
      301 cattcatttt caagacatct cgattcatat tcaagatagg aatcttttta tcaacatcac
      361 tttattcgta ttttcctcta ccataaaagc ttcacgctcc acaaaattgt tttatacact
      421 actattcagc ccattcaacg accaatgaat
//
LOCUS       DAZAFF010000251          415 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000251 DAZAFF010000000
VERSION     DAZAFF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.265"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..339)
                     /locus_tag="ACG9BB_003037"
     CDS             complement(<1..339)
                     /locus_tag="ACG9BB_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2838877.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAZAFF010000252          414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000252 DAZAFF010000000
VERSION     DAZAFF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.266"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>414)
                     /locus_tag="ACG9BB_003038"
     CDS             complement(109..>414)
                     /locus_tag="ACG9BB_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN2838878.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          116 a           96 c           82 g          120 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggt
//
LOCUS       DAZAFF010000253          408 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000253 DAZAFF010000000
VERSION     DAZAFF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.267"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>408
                     /locus_tag="ACG9BB_003039"
     CDS             <1..>408
                     /locus_tag="ACG9BB_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFN2838879.1"
                     /translation="QDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQEY
                     GKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQA
                     AYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL"
BASE COUNT          138 a           67 c           83 g          120 t
ORIGIN      
        1 gcaggatgtg attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga
       61 agttcaagaa atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg
      121 ggtgcaagaa tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt
      181 ttattcatgg aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta
      241 tcgagccatc gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac
      301 acaagcagcc tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaaggttgt
      361 agtcacagat aaagccccct ctattacaag tgcctttaag aaactaaa
//
LOCUS       DAZAFF010000254          379 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000254 DAZAFF010000000
VERSION     DAZAFF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.268"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..379
                     /locus_tag="ACG9BB_003040"
                     /pseudo
     CDS             46..379
                     /locus_tag="ACG9BB_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          152 a           72 c           73 g           82 t
ORIGIN      
        1 aaataaggtg tgtagaataa taaaaaacac actggaggat ttttcatgcc aagacgtcaa
       61 cgaagaacct attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag
      121 cctcgcgcag aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg
      181 aagcaagcac aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca
      241 gaattgatcg cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag
      301 caagcggcgc tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt
      361 ccatatcagc gatgtgcaa
//
LOCUS       DAZAFF010000255          345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000255 DAZAFF010000000
VERSION     DAZAFF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.270"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          113 a           65 c           56 g          111 t
ORIGIN      
        1 atctgttcta acattctcaa ctttgtcagt ttaccatagg aaaatgggaa aatctacgaa
       61 agctctcaac agaatcgtcc tacttaaata cgcttttctt taataataaa taagttggtt
      121 gtttttgtta tttgcgcttt cacttttcgg aataaataat gtgtatttat atggaataaa
      181 taaaaaaaga ggtcgtgact aaaaacaagt cacaacctct ggtaaacggt gttctcctga
      241 caactcctcg gtcacaagac acaatattca acaaacatga gaagctattt tttgaaattg
      301 ctctttgtga ctcagagtga aacggcaggc tcacgacctc tttct
//
LOCUS       DAZAFF010000256          337 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000256 DAZAFF010000000
VERSION     DAZAFF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.271"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..108
                     /locus_tag="ACG9BB_003041"
     CDS             <1..108
                     /locus_tag="ACG9BB_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFN2838880.1"
                     /translation="FTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQF"
     gene            212..>337
                     /locus_tag="ACG9BB_003042"
     CDS             212..>337
                     /locus_tag="ACG9BB_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFN2838881.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
BASE COUNT          140 a           31 c           65 g          101 t
ORIGIN      
        1 ttcactgaat atcaagagga attaataaga aatttgagtg aaattggaag aactgctgga
       61 ataaatggtt tatatcgagc agattatgga gataaatctc aattttaatg atacttataa
      121 tattaaatat gtctcaaaat tttttgatta gaaaaatagg tatattgaat cagtgacata
      181 aaagtattta tagtttgtag ggggaatcgt aatggaagaa aaaaatagac taaatgctaa
      241 acaatgttca gatcaagagc ttaaaaaaat taaaggaggt gctggaacaa aacctcaagg
      301 aaaaccggca tcaaatttag tggaatgtgt tttttca
//
LOCUS       DAZAFF010000257          328 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000257 DAZAFF010000000
VERSION     DAZAFF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.272"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>328
                     /locus_tag="ACG9BB_003043"
     CDS             <1..>328
                     /locus_tag="ACG9BB_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838882.1"
                     /translation="CSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKRKLSHGYF
                     VSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGNICPGFMD
                     QIIENES"
BASE COUNT          136 a           32 c           57 g          103 t
ORIGIN      
        1 tgttctaatt gtggtacatt attttactat gaggaagtta gaaatattgt aaaacataga
       61 aaaaaagaaa tgcctatacg ttgtccaaat tgcaatgaag ttattaagcg aaagttgtct
      121 catggttatt ttgtcagcta cgaactatat gaagaaagta aaataaatct gaaaaaaaaa
      181 ccaaaatact ataaaaattt gaaaactgga gctattataa ctgaaaaaga atttaaaaat
      241 ttagtgactg aagaagaaag caggttttat aatttgtctg gtaatatttg ccctggattt
      301 atggatcaga ttattgaaaa tgagtcaa
//
LOCUS       DAZAFF010000258          307 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000258 DAZAFF010000000
VERSION     DAZAFF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.273"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG9BB_003044"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACG9BB_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFN2838883.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           96 a           45 c           50 g          116 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttat
//
LOCUS       DAZAFF010000259          302 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000259 DAZAFF010000000
VERSION     DAZAFF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.274"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          121 a           32 c           52 g           97 t
ORIGIN      
        1 ttgctatatt attatttgtt ctaaatgatt cctttaaggt gtcactaaaa attataaaaa
       61 atagtgttga cgcacttttg atgtcatgtt ataataacga agtcgtttgg atgagatagc
      121 taataaagct tgattttatc gaaaaaaact tcgaaaaagt tattgacaaa taacaataaa
      181 gatggtatga taatcaagtc gctgctaatg agtggcaaag acgtttagaa aaaataaaca
      241 aagaagttgt tgacattctc ttctgaatct gataagatta aagagttgtt aaaacaaaaa
      301 ga
//
LOCUS       DAZAFF010000260          282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000260 DAZAFF010000000
VERSION     DAZAFF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.275"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACG9BB_003045"
     CDS             <1..>282
                     /locus_tag="ACG9BB_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFN2838884.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAZAFF010000261          282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000261 DAZAFF010000000
VERSION     DAZAFF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.276"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>282)
                     /locus_tag="ACG9BB_003046"
     CDS             complement(<1..>282)
                     /locus_tag="ACG9BB_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN2838885.1"
                     /translation="KQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFD
                     NAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYE"
BASE COUNT           90 a           55 c           48 g           89 t
ORIGIN      
        1 ctcgtagcca agtgtaccgt gcaaccgaag gtggttccac caattgacat agtcaaataa
       61 ctccaaatcc aattgttgta aggtttcaaa tgtgtattga tagacaaatt ctactttcaa
      121 cgacttataa gttgattcag ctacggcatt atcaaaagga cagcctttat gactcaatga
      181 tcgattgatg tcaaaagttg ttaataattc atcaatagct tggttatcaa actcttttcc
      241 acgatcagta tgaaaaatct caacctctgt cagaggttgt tt
//
LOCUS       DAZAFF010000262          265 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000262 DAZAFF010000000
VERSION     DAZAFF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.277"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>265)
                     /locus_tag="ACG9BB_003047"
     CDS             complement(<1..>265)
                     /locus_tag="ACG9BB_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFN2838886.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFV"
BASE COUNT           92 a           47 c           40 g           86 t
ORIGIN      
        1 acgaaagtat actttatata gtcataacgc cgatgatcgc tacgtgcgtc cggcacgtca
       61 gtcacgatat caaacaaaaa gtatacaccc ttcttcattc tagttttcgc agcaggaatt
      121 ttgaaatagt tcttattaga atagtagaga ttgattaata agctatcttc gattgctaaa
      181 aagaaaactt ctgaattcca tactttataa aaatctttga taaatctatt cgaagggtca
      241 aatttaaacc ataattgtgt ctcat
//
LOCUS       DAZAFF010000263          253 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000263 DAZAFF010000000
VERSION     DAZAFF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.278"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>253
                     /locus_tag="ACG9BB_003048"
     CDS             <1..>253
                     /locus_tag="ACG9BB_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFN2838887.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWT"
BASE COUNT           80 a           40 c           58 g           75 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga cat
//
LOCUS       DAZAFF010000264          247 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000264 DAZAFF010000000
VERSION     DAZAFF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.279"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>247
                     /locus_tag="ACG9BB_003049"
     CDS             <1..>247
                     /locus_tag="ACG9BB_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFN2838888.1"
                     /translation="PIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQLSRRRI
                     GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTF"
BASE COUNT           80 a           51 c           61 g           55 t
ORIGIN      
        1 ccgatcgaaa atgagtccga cttagaagag atcgttcagg aagagtttat tcgaaaccga
       61 aaggcttacg gtacccgaaa attgaagaag tgtttagcaa agcgtgggct tcaactcagt
      121 cggcgccgaa tcggtcgaat catgaaacgc cgcggattga catctaccta tacgatcgct
      181 cattttaaag ggcaacgaac agcttgtaat gaagcgaaaa cagcgaatgt attagatcgg
      241 accttta
//
LOCUS       DAZAFF010000265          235 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000265 DAZAFF010000000
VERSION     DAZAFF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.282"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>235)
                     /locus_tag="ACG9BB_003050"
     CDS             complement(<1..>235)
                     /locus_tag="ACG9BB_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN2838889.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT           69 a           45 c           36 g           85 t
ORIGIN      
        1 atatcctaaa gaatatctca aagttccttc aaaacgtcgt tgtgctcgac ttaatagaac
       61 actgtaagcc agtaatatgc tttcaaatct actctctagc cctttaacag aacgtgagtt
      121 gaaattttga caacctaatt tttctaagga agaaaaaaca gtctcaactg tttttctttg
      181 ttttccaagt aagctgtggt ctagtttttc tgatttatcc atgtttttac gtggc
//
LOCUS       DAZAFF010000266          233 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000266 DAZAFF010000000
VERSION     DAZAFF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.283"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DAZAFF010000267          231 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000267 DAZAFF010000000
VERSION     DAZAFF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.284"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..201
                     /locus_tag="ACG9BB_003051"
     CDS             <1..201
                     /locus_tag="ACG9BB_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFN2838890.1"
                     /translation="YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTR
                     KEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT           77 a           45 c           45 g           64 t
ORIGIN      
        1 tacctgaata atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat
       61 cgaagtttac gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat
      121 aagaaaaccc gaaaagaagg cactctcttc gggttttcgg tctgtactga aatcaaggta
      181 ttattgggaa tcccagctta aatcatagat accgtaaggg attttattct t
//
LOCUS       DAZAFF010000268          229 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000268 DAZAFF010000000
VERSION     DAZAFF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.285"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>229
                     /locus_tag="ACG9BB_003052"
     CDS             22..>229
                     /locus_tag="ACG9BB_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFN2838891.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTE"
BASE COUNT           86 a           43 c           44 g           56 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaa
//
LOCUS       DAZAFF010000269          227 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000269 DAZAFF010000000
VERSION     DAZAFF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.denovo.286"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>227)
                     /locus_tag="ACG9BB_003053"
     rRNA            complement(<1..>227)
                     /locus_tag="ACG9BB_003053"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           48 a           77 c           45 g           57 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccag
//
LOCUS       DAZAFF010000270        13213 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.guided.076, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000270 DAZAFF010000000
VERSION     DAZAFF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.guided.076"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACG9BB_003054"
     CDS             complement(204..2162)
                     /gene="nagE"
                     /locus_tag="ACG9BB_003054"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFN2838892.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="ACG9BB_003055"
     CDS             complement(2159..3031)
                     /locus_tag="ACG9BB_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFN2838893.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="ACG9BB_003056"
     CDS             complement(3373..4245)
                     /locus_tag="ACG9BB_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFN2838894.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="ACG9BB_003057"
     CDS             4373..4942
                     /locus_tag="ACG9BB_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFN2838895.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="ACG9BB_003058"
     CDS             5009..5746
                     /locus_tag="ACG9BB_003058"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFN2838896.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="ACG9BB_003059"
     CDS             5977..7245
                     /locus_tag="ACG9BB_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFN2838897.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="ACG9BB_003060"
     CDS             7732..8196
                     /locus_tag="ACG9BB_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFN2838898.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="ACG9BB_003061"
     CDS             8180..8809
                     /locus_tag="ACG9BB_003061"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFN2838899.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACG9BB_003062"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="ACG9BB_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFN2838900.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="ACG9BB_003063"
     CDS             10688..11134
                     /locus_tag="ACG9BB_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFN2838901.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="ACG9BB_003064"
     CDS             complement(11333..11848)
                     /locus_tag="ACG9BB_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFN2838902.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="ACG9BB_003065"
     CDS             11965..13167
                     /locus_tag="ACG9BB_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFN2838903.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAZAFF010000271         9585 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.guided.100, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000271 DAZAFF010000000
VERSION     DAZAFF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.guided.100"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(241..1182)
                     /locus_tag="ACG9BB_003066"
     CDS             complement(241..1182)
                     /locus_tag="ACG9BB_003066"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFN2838904.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1308..2429
                     /locus_tag="ACG9BB_003067"
     CDS             1308..2429
                     /locus_tag="ACG9BB_003067"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFN2838905.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            2687..4189
                     /gene="eat(A)"
                     /locus_tag="ACG9BB_003068"
     CDS             2687..4189
                     /gene="eat(A)"
                     /locus_tag="ACG9BB_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFN2838906.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4256..4783
                     /locus_tag="ACG9BB_003069"
     CDS             4256..4783
                     /locus_tag="ACG9BB_003069"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFN2838907.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            4994..6055
                     /locus_tag="ACG9BB_003070"
     CDS             4994..6055
                     /locus_tag="ACG9BB_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFN2838908.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6058..6954
                     /gene="murQ"
                     /locus_tag="ACG9BB_003071"
     CDS             6058..6954
                     /gene="murQ"
                     /locus_tag="ACG9BB_003071"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFN2838909.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            6968..8950
                     /locus_tag="ACG9BB_003072"
     CDS             6968..8950
                     /locus_tag="ACG9BB_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFN2838910.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9015..9461)
                     /gene="nrdI"
                     /locus_tag="ACG9BB_003073"
     CDS             complement(9015..9461)
                     /gene="nrdI"
                     /locus_tag="ACG9BB_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFN2838911.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3119 a         1707 c         2088 g         2671 t
ORIGIN      
        1 tgcagaaaat tgggcaaaac gatttatcac taacgattcc actatttatg agcaatacga
       61 atatgacctc tcctctgttg aaaacaaata aaaaacctac attttgaaga gattatgtac
      121 tgaaacaaac aagcacaatt cttatggcat catgagagtt gtgttttctt tttgatcttt
      181 tattgggctt aaaaaaagag aaccagctgc tgattcagct gattctcatc agagagcctt
      241 ttattctgcg ccaaggattc cttcttttgc atggactaat tgggtcaaaa aggcacaata
      301 tggtgtagca atatcatatt tttgtccttt tcgccaaatg gcaccattga tgtagtcgat
      361 ttcagtcaaa cgatggtttt tgatcaggtc ttggtacata gatgggtaat gcaacccgat
      421 gttgtcagga tcataagtag aagcaatatg ttggtacact tcttcttgat ctagtatgat
      481 tccttctttt gctgcgatag aagcaaattc agatacgatt gttcgaacca tgctctctgc
      541 cgctttttgc gcgcctagtt cagcaatatt acaatccaaa atcgtgcata aaccattcaa
      601 tgttccattt acacaagctt ttcgccaaat ggaataacga acgttatggc tatagatcgg
      661 atttaaattt gcttcttgaa agacttctac aacttttttt gtaaatgctt cgccttctgg
      721 ttccaagttc tctagttcga tttctccatc acctaacaat gtgacttttc cgggaccagc
      781 tagaccagct gtccacatcg tgatcccaaa taagatattt ttctttggca catatttttc
      841 cagtacatct tcatgaccta atccatttag taaacacaaa acataagtat tgtcagtaat
      901 aatggattgg atcgaacaaa acatatcatc taattgattg gcttttgtta atgctacgat
      961 caagtccacg tgttcgttgc tttcgatgat ttcttctggt gaatagattg gcagttttgc
     1021 gaccacttct ttaccgttaa agtcagcaat caacccattt gtacgaatgg cttcaatgtg
     1081 agcaggccaa cgatcgatca agaggacctc atttccgctt tgatgtagca tcaatccgat
     1141 tcggctgccc attgctccgg ccccagctat tgcgattttc atgttttttc tctcctttat
     1201 tcaatgagtt cccttttagt atatagaaaa atggaagaaa atagcattgt tttgtttgaa
     1261 aaaaatgaaa aaaagtgtct tacttaagaa gaaagtgagg gatacagatg gatattttgg
     1321 cggcaattga ttcatttaaa ggaagtgctt cttcatttga actaaatcag gcagttctaa
     1381 atggctggga agatcgtaaa ggaagaaaaa tcaatgtgcc gattgcagat ggcggagaaa
     1441 atactgcaga agcaatctat tacgcgctca ggggaacatg gcgtaccatt gatgggatgg
     1501 atctcttatt ccgtcagcga aaaatcagct atttattgac aacttggcaa ggaaaaaaca
     1561 ccgcagtcat cgaatctgca caagcattgg ggttagatct acttgataaa ccaacggatc
     1621 aaactatccg acttgcttcc agttatgcct taggagaact tattaaagat gcactgcttc
     1681 aagaagtaga acaaattatt gtcactttag gaggaagcgg gtgtagcgat ggtggtctag
     1741 gattactcca atccttgggt gcttctttag aagggtacac agaaggaaat cctttattat
     1801 ctactaaaaa acttcatttt gatcgaagaa acgttcttgt ttctaaagta aaattgatta
     1861 tcgctgcaga tgttaccaat ccatatacag gtcaacaagg agctgcttgg caatttggaa
     1921 gacaaaaagg aggaatagat acgacgctag ctttcttaga taaacaagca ggaaagattg
     1981 ctcagcaaat aaaagatttc tgggaggtgg atttagatga tattccagga agtggagcag
     2041 caggcggtat tggtggagcc ctttttttgt tgggagcgaa gatggtttct ggtttcgatt
     2101 tagtcagcca aatcactgga ttggaaaaaa agatcgaaca agcagatgtc atcattacag
     2161 gagaagggcg gatcgatcag caaacgatcc acggaaaagt tccatacggt gtagcagtgc
     2221 ttgcaaaaaa atatcgaaaa ccaataatcg ctatttgcgg aagtagagac caagagctag
     2281 aaggactttc tgattttctc ccaattgtct taagtattca tttgggtcca ataggtttag
     2341 aagaagcaat ggaacacagg caaacattag aaaaaacaaa aatcttagga cagacactta
     2401 gcagattttt caccatcctc ccgaagtagt tgacaaaatc attttcttta tgctatcgta
     2461 atttcaaata taggacaaag tgagggatcc gtgcattgaa aaattaagtc gattgatgaa
     2521 agctgcattc agactgtacc ttgtggagac aaggaagcag tgtgcagatt ttcgggattg
     2581 gcttggtttt ttcgatgtat gggtcttttt gtgtgtatcc aacccttgca tcctctttag
     2641 ccagttctga aaataggact ggtctttttt tacgaggtga tggaaaatgt ctaaaatcga
     2701 aataaaaaat ctgacattcg gctacgacag ccaaggcaca ttattatttg aacaagcaaa
     2761 tctaaatttt gacacacaat ggaaactagg acttatcgga cgaaacggtc gaggaaagac
     2821 aactttactg aatattctac aaaacaaact accttatcaa gggcaagtaa tccatcagca
     2881 agaatttgcc tatttcccgc aacagacaaa agataaagaa cgtttaacct attacgtgtt
     2941 aaatgatatt acggattttg agatatggga aatcgaaaga gagctccaat tgatgcaaac
     3001 agatcctgaa atcttatgga gagaattcag cacactatcg gggggagaga agacaaaagt
     3061 cctactggca cttttatttg tggatgacac tcatttcccg ttaatcgatg aaccaacgaa
     3121 tcatttggat atctctggta gaaaacaagt agcggcttat ttgaaaaaga aaaaacaagg
     3181 cttcatcgtg gtcagccatg accggggatt tatcgatgaa gtagtggacc atgttttagc
     3241 aatcgaaaaa agtcaactgg aactttatca agggaatttc tctatctatg aagaacagaa
     3301 aaaacttcgt gatgaatttg aaatggctca aaatgaaaaa ttgaaaaaag aagtcagtag
     3361 gctaaagaaa acagcagctg aaaaagccga atggtctcgt tcccgagaag gagataaaac
     3421 aaagaaacaa gtcggattca tcgatactga atctagacga gtgaataaag gagcagtggg
     3481 tgctgatgct gcacggacga tgaaacgatc caaagcaatc gtgaatcgga tggagaccca
     3541 gatcagcgag aaagaaaaac tattaaaaga tatcgaatat atcgattctt tgacgatgaa
     3601 tagccaagcg tctcaccata agcgactttt aagcgtagaa gatcttcaat tagggtatga
     3661 aaatctgtta ttcgagccaa ttcattttac aatcgagcct catcagcggg tggcgatttc
     3721 aggtcctaac ggtgcaggaa agtcatccat tatccattat cttctggggg cattcaacgg
     3781 caaggttata ggagaaaaat accagccaaa acatctgagc attagttatg caagccaaaa
     3841 ttatgaagac aatcgaggaa cgttggcgga atttgcagag aaaaaccaag tagactacca
     3901 agcatttttg aacaacctcc gaaagcttgg gatggaaaga gatgtttttc ataacaagat
     3961 cgagcagatg agtatgggcc aacggaaaaa agtggaattg gctaaatctt tatcacagcc
     4021 agctgaacta tatacatggg atgaaccatt gaattatttg gatgtcttca atcaagaaca
     4081 attagaacaa ctgatcttga acgtgaaacc tgccatgtta ctagtggaac atgatcaaac
     4141 cttcctggat aaagtatcta ctgagattat ttctcttgag agaatctaac ccttgtaatc
     4201 tcaagaacaa caaggtatgg ttaaggggaa ctttgccaac gaaggagaga atcacatgct
     4261 ttatgtaacg agacacggag aaacaacttg gaacgcacaa ggattagttt gcggacgggc
     4321 agatgtgccg ctgactgaaa aaggacagat gcaggcacag aaactagcag aaaaggtagt
     4381 agatttgcct gttccaatca cgaaaatcat ccattcccct cttcaacgag caagagatac
     4441 agcacaagca gttgctgatc gtctcagctt gccgctgacc gttgatgaac gattggtaga
     4501 aatggatttt ggggattatg atggactgcc aagcaaagat gagaactttc aaaaagcacg
     4561 tttagcgttc gctgtacgat tcccaaatgg ggagtcagtc ttagatgtgt atgcacggat
     4621 cgttccttta ttgaaagaat gcatcgaaga cgaagaaaat gtctatctgt tagtttgcca
     4681 taatgccttg ataagagtga tcaatgctta ttttcatccg atgcctaatg aggggttctt
     4741 tacattcatg gtagataata cagaattggt ttcttatgag taattatgtt tttgtgttat
     4801 ttaaataaaa tatttcaagg cactgctttt gcttagcagt gcctttttta ggttatgaca
     4861 attcttttga taatataaac tcaaccttta gaaatgaaat ttaattattt taataaaagt
     4921 attgaaattt tgtttcttta gatatatact ctagtcatag aaatgaagac ggatacagaa
     4981 aaagagggat aatatgtttg gcttttcaat atttatgaac gattctttgt cagaagagaa
     5041 gaaacagtat atccgccaaa tggcatccaa tggctttgtc ggtattttca cttccatgca
     5101 tataccagaa gatgatgttt cggcatatca aaagcgattg atcgaactag gaagcgaagc
     5161 gaaaaagaat aaactagaat tgatggtcga tatttcagga gaggcattgg aacgagcggg
     5221 tttttcatta gaagatctaa cgcctttgaa agcaattggt gtaacaggtt taagaatgga
     5281 ttatcatatt tccaatcagc agattgccga ctggtcacat caattgaaaa tcagtttgaa
     5341 tgctagcacg atcacgccta aagatatcga tgaattgaaa gaagcagaag ctgatttctc
     5401 acagattgaa gcttggcata attattatcc acgtcccgaa acagggctgg acaaagaatg
     5461 gtatcagaaa aaaaatcaat ggctcaaaat tcaaggtctc cttgtccaag gttttgtacc
     5521 aggagataca gagttgagag gcccactata tcaaggattg ccaactttgg aagaacatcg
     5581 agggatccat cctcttgcag gcgctctaga tctattagca agtaacacgg atattgttta
     5641 catcggagat gcaggtctgt cagaaaacgt tcaggagcaa tttgcttctt tccaaaaaga
     5701 acagacagtc ttgctccata cggaacctgt agatgaagaa ttttacgaat atatattagg
     5761 gaagcatacg aatcggcaag acgatgcaag agatgtcata cgaagcgtag atgcacgttt
     5821 tcgagagatt ccgcctatcc cagcaagaaa tacagctacg cgtatgaaag ggtctattac
     5881 tttagataac gaaaagtatt tgcgctatat gggtgagatc caattaacca aatatgattt
     5941 gccagcggat gaaaaagtga atgtagtagc taaagttatc aaagaagagc taccattgat
     6001 caaccaaatc aaagctggta tgaactatca atttataaga aaagaaggaa gataaaaatg
     6061 agcaaaatca atttagagaa cctaacaacg gaacgcagaa accaagaaac ttttggatta
     6121 gatgagatga gtatttctca agccgttcaa ctaatgaaca aagaagatca gaaagtggcc
     6181 gctgcggttg aaaaagaact gacaagtatc gaaccagtga ttgctaacac gattgagtcc
     6241 tttaaaaaag gtggacgtct gatttatatg ggagccggaa caagtggaag actaggtgtg
     6301 ttagatgctg ctgaatgcgt accgacattc ggtgtagaac cggaaatggt cataggtcta
     6361 attgctggtg gagaagaagc aatgactgta gctgtcgaag gggccgaaga ttcttatgag
     6421 ctagggcaag aagatctaat caagctgaat ttgacaaaaa atgatatagt tattggtatc
     6481 gcagctagcg gtcgtacacc atatgtgatc ggtggattag attacgcaaa atcgatcggt
     6541 gctgtcacag gaacgatttc ttgcaataaa caagccgaaa tcagtaaaca cgcggatttc
     6601 ccaatcgaag tcgatgcagg tccagaattt ttgacaggct ctactcgatt gaagtcagga
     6661 acagcacaaa aacttatctt gaatatgatt tctactatca gtatgattgg tataggtaaa
     6721 gtattcaata acttgatggt cgatgtcaaa ccaacaaatg aaaaactaat cgaacgctca
     6781 aaacgaatca tcatgcaagc gacagaagct gattacgaaa cagctgaaaa gtactttgaa
     6841 gaagcagatc aaaatgttaa gctggctatc gtcatgattt tgacaaatag tgataaagag
     6901 gaagctcaag caaaattagc tgaagcaaac ggatttgtaa aagggacctt atgaggggga
     6961 aaagaccatg gcaaaaaacg aactgacttt ggaagaactg gcaaaacaaa tttataaagg
     7021 ggcaggcggc atgggcaatg tagaaagcat tgtccactgc atgactcgtg tgcgtatgaa
     7081 gatcatcaat gatgatcttg taaatgtctc tgaattaaaa gcgattccag gagtgcttgg
     7141 tgtagtagaa gatgaacagc ttcaagtcat catcggacct gggaaagtga acaaagtagc
     7201 acagcaaatg gttgatttgg caggcgtgaa attaggagaa aaactttctt ctgtaagtac
     7261 agctacagca aattcatcag catctggaaa agaccgtgta aacgaacggg cacaagaaat
     7321 gaaagcggca caaaaatcga aacaaaaacc atctaaaatc aaatcggtat taaaagacat
     7381 ttctaatatc tttgtgccaa tgattcctgc atttgtcggt actgggatcg tggcagggat
     7441 tgccgcagtt ttagcaaact tagtcacagc cggaacactt gatgcagcaa catggcaaca
     7501 atatatcgat gtgatgaaca tcttgaaaaa cgggatgttt agctacctag ttatctatac
     7561 aggtatcaat gctgcacaag tttttggtgc aacaccaact cttggtggag ttatcggggc
     7621 cgttgtgatg ctgacaggga tgaatccgga agcaccactg aaaaacttat ttactggtga
     7681 agctcttgca gctggacaag gtgggatcct aggtgtcatc ttcgctgttt ggctattgtc
     7741 aatcgttgag aaaaaactgc ataaagtagt acctgatgcg gtagatatca tcgttacacc
     7801 aactctttca ttgattgcca tcggtctgat tgaaattttc ttcatcatgc ctttagcagg
     7861 tttcatttct gatggcatgg tcggtgggat caactgggta cttggcgttg gtggagcatt
     7921 tgccggcttc gttctaggca cattattctt gccgatggtt atgttcggac tgcatcaaat
     7981 tttgactcca atccatgtgc aaatgattga tgaaactggt cgtacgctat tgcttccaat
     8041 tcttgcgatg gctggtgcag gacaagtggg tgcagcattg gcattatgga ttcgctgcaa
     8101 aaaagataaa gaattaacag aaatgatcaa aggagcttta ccagtcggga tcctaggaat
     8161 cggcgaacca ttgatttacg gagtaacatt acctctagga cgcccattta ttactgcttg
     8221 tatcggtgga ggtatcggcg gggcagtgat cggaatgatc ggaaatgtcg gggctatcgc
     8281 tattggacct tcaggagccg ctttgatccc attgatctct gacggtaaat ggttaggcta
     8341 catccttgga ttattggcag cttatgctgg tggcttcgtt gctacattct tcttcggtat
     8401 tcctaaagac cgtttagaaa aagaagaatt agcgactgaa acagccacag taaatgaagt
     8461 gcctgaaaaa gtagtacctc aaacttcagc tgaaagtgtg actatcctct catcagttgc
     8521 tgacggaaca gttgtttctt tagataaagc aagtgatcca gtttttgcac aaaagatgat
     8581 gggtgaaggc tactttgtag aaccagaaaa cggacatatc tattctccag ttactggggt
     8641 agtcagctct gtcttcccaa cgaaacacgc tattgggatc acaacgccaa atggtttaga
     8701 aatcttgctt catatgggaa tcaacacagt tgacttaggc ggtacgccat ttgatgtgaa
     8761 agtaacagaa gggcagcaag tgacacctga tacgcttgta gcagaagctg acttagaagc
     8821 gatcaaagca gcaggcaaag aaacatccat gatcgtcttg gtaaccaata tggatcgtgt
     8881 aaaaaacttt gtcttggaaa aaacaggtaa agcaaaagca aaagcacctg tgatggatgt
     8941 caaagcttaa tatgatagaa acaatagaaa cgaaagaaga aactgaaatc tatttttcag
     9001 cttcttcttt tttattattt attgttttct gctaaaatct gataaattcg tgtcacatca
     9061 gatggtgttc cccgtaattc ataatctgcc aagaaaggaa agccaaatgt ttgtgcatat
     9121 tgtttggctg ttagacaata ctgattattg aagtttttat ttccgctacc gactacacca
     9181 agacaaagtt tatgattgtc ttcatgttcc agatattcac gcatcgtttc ggtcaagatt
     9241 tctgtatcac cgttatctaa accatttccg ccgtctaaat aagtaggaac gaaagtaaaa
     9301 aatggttctg tttctttcgt aaaatcacta ttttcatgga tttcttttaa agtaacttca
     9361 ggattgacag gatcttctgc atgcattttt tcagcatatt ctgctagatg tttggcaaag
     9421 gcacgcgtat ttcctgaaat cgatatatat aaaatgttca tggatcatcc tccaattctt
     9481 tccttttagt atagcatctt ttttaagttt tttggtggtg taatttggaa agggactagg
     9541 cgctttgctt gtataatgag aaaaaagccg gtagaattat atttc
//
LOCUS       DAZAFF010000272         7565 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.guided.117, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000272 DAZAFF010000000
VERSION     DAZAFF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.guided.117"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..124)
                     /locus_tag="ACG9BB_003074"
     CDS             complement(<1..124)
                     /locus_tag="ACG9BB_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFN2838912.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIR"
     gene            complement(117..1256)
                     /locus_tag="ACG9BB_003075"
     CDS             complement(117..1256)
                     /locus_tag="ACG9BB_003075"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFN2838913.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(1461..2654)
                     /locus_tag="ACG9BB_003076"
     CDS             complement(1461..2654)
                     /locus_tag="ACG9BB_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFN2838914.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(2733..2939)
                     /locus_tag="ACG9BB_003077"
     CDS             complement(2733..2939)
                     /locus_tag="ACG9BB_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFN2838915.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(2932..4074)
                     /locus_tag="ACG9BB_003078"
     CDS             complement(2932..4074)
                     /locus_tag="ACG9BB_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFN2838916.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            4586..5221
                     /locus_tag="ACG9BB_003079"
     CDS             4586..5221
                     /locus_tag="ACG9BB_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFN2838917.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            5357..5449
                     /locus_tag="ACG9BB_003080"
                     /pseudo
     CDS             5357..5449
                     /locus_tag="ACG9BB_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            5563..6198
                     /locus_tag="ACG9BB_003081"
     CDS             5563..6198
                     /locus_tag="ACG9BB_003081"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFN2838918.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            6305..7543
                     /gene="mef(H)"
                     /locus_tag="ACG9BB_003082"
                     /gene_synonym="mefH"
     CDS             6305..7543
                     /gene="mef(H)"
                     /locus_tag="ACG9BB_003082"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFN2838919.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
BASE COUNT         2283 a         1384 c         1391 g         2507 t
ORIGIN      
        1 ttcggatttt tactgctgtt tcatctttta cagttgaaat aaaattatct agtgtccgaa
       61 aaattttatc actgtttgcc gtcacaatac taataaggat cggctggttt aatttgttat
      121 tcatctatat gacggttcca tttcttcaaa tatttctgtt ttacttgcgt acggtactct
      181 ttattcccta aaaatcgcaa tgtttttaaa ataaactgat gtttctcatt gatcagacca
      241 atcaattcaa ataataaaat tgcagcaaac aacatggcaa taatcaatat gatcgttccg
      301 attcttccag tataattcat gaccatagct gtgaaagaaa acatcaaagc catcgcataa
      361 atcgtcagga cagcaccttt atgagtaaag cctaaactga ttagtcggtg atgaagatgt
      421 tttttatctg ctgtagaaaa agaaacacgg tttgcttttc tcctaatgat agcaaaaaac
      481 gtgtctgtta ccggcactcc catgacgatc agcaaagaaa tcgatgaaac aaaagtcacg
      541 ttcttcagcc cttgtagtga aaggacggcc atcataaacc ctaggaatag agctcctgta
      601 tcgcctaaat aaatctttgc cggataaaaa ttgtaaggaa aaaaaccgat aatacttgct
      661 acaagacaga aaataacaac tggaatatac actgtagaag catggaggaa aaaatatcca
      721 atgattccta tcgtaattaa accgatcaat gaaatccctg ccgccagccc atccaaaccg
      781 tcaatcagat tgaccgcatt cgtgataccg aagatccaaa aaatcgttac gggaaaacta
      841 agccagccta gttggatata tccaataaaa ggaagcgtga aggaatcaat acgtatatct
      901 gctacaaaat aaataataag agaagcaatc aagatcccta tacttttttg tttaggtgtc
      961 agctcatatt tatcatcgag cactcctgtg actatgatta ctaaagaagc aatcaagatc
     1021 ggtacaatat acgattgagg gataatttta cggaataaga ataaagtcga tataacaaat
     1081 gaaataaaga taggcagtcc gcccgctgaa ggcattggaa ctttatttat tcttctttca
     1141 tttggtttgt cgaccatacc tgtttgtact gaaatgattt tgaatacagg tgttaatatc
     1201 agtgataata aaattgtcag aaaaaatctt ataaccatac taaaagaaac aagcatatgg
     1261 gcacctactt taattaagag tcataattat tataaattat atatgtcttt ttgactatct
     1321 ttttatatct tctcaaatgt ccgcaaagcc ttattttatc ggctctacgg gcattttgct
     1381 tttgtggtaa acctcgcata tctaggtcta tcttcttata ttttccctgt caatcgtggt
     1441 taaaatcgtg gtaaatactt ctatgcgatt agacgctgaa cctctgattt tgcggtatct
     1501 atggacgcat gagcgtacca gttcattgtg atactgatgt ttgaatgccc catgatatac
     1561 tgtaaatctt tcgggttcat gttcttgctt gccagccttg tgcagaatgt atggcgtagc
     1621 gtatgcggtg taatatgtgg caagggattg tccttgtggt gcttgttgta tttctttacc
     1681 atacggataa ataaggcgtt ataatcaatg gcaacttttg gattaccttt cggattgata
     1741 aacacaaaac cgctgtgtcc gtctacctct atcgacttta tctttggtcg gctgttgata
     1801 acccgttgaa atgcctgtat cgtttctttg cttaatggca cttgccttat cccgcttttc
     1861 gtcttaggcg tttcaatata atagccctgt tccttactct ttagtaactg gtggtcgata
     1921 atcacaactt cattcttgaa atcaatgtcg gctactgtca gtccgcacag ttccgagata
     1981 cgaagtcctg tctttaacag tatcagcaca tcatcataat gcttgtgata cacgttgtcc
     2041 gtcttaataa atgacaataa ggcttgctcc tgttcctctg tcaatgcgac tttctctttg
     2101 gtatcatttt ctatgacttc acttagcttg aaatcaaaag ggtttttcct cacataatca
     2161 tcttgtatgg ctatgtagaa tgacgctttt aacgagcgtt tatggttgtt aatggtattg
     2221 taggaaaagc ctttgtcttt catgcgtaac gcccattctt tagcgtcaga gggttttatc
     2281 gtatcaatgc tcctagcacc taacttgtcc tcttttaata accgcatgag ttgttcccgt
     2341 tgtttctgtg tgcttttctt cacatttgtc ctctgtgcgt tctgtttggc atagagttgg
     2401 caaagcgtca ttttcttgcc tgtgctgtcg ataccgtcct caatatcccg tcttatctgc
     2461 tgttccagtt ctcgaagtga gggtttttcc cgttttccct ttggtgtcgg gtctgtgggt
     2521 gtcaatctcc aagcatacac atattttgtg tttcccaatg cgtctacata tttatataag
     2581 tattttccgt ctgttcgttg gctctctcca gtatgcagga tacgaccttt gttatcccgt
     2641 cttttttctt tcatggtgtc tgctcctttc cttgttggaa agagccttga tatgacttgt
     2701 gttcatcata acacatacaa ggctcatttg cattagattg cgtccaattt atcaataacc
     2761 ttttcaaact gtcggcgttt aatctgtatg cggttgccat tcataatgag ccagccagcg
     2821 tccttgtttt cctctgccaa tcgtcttaac ttgttttctc caatgcggaa atactttgac
     2881 gcttcttcaa tggtaagggt gtatttttcc catacgggaa tatcctgtgt attactcata
     2941 aaacaccccc tcactttcga ttacgtctga tatgtccgta cacatctgtt ccacaagcat
     3001 ttcctgtttt tcagaaaggt gggtacattc atcaatcatt ttcatgtagt ttgaacctgt
     3061 cagcctgtcg atttcctcta tcatcacgat tgacggcatg acctgtcttt ccagccattg
     3121 cagagtgcgt tccatattga caggtacagg gttcatttca aaaggtaaac tgtcatggga
     3181 aatgaatatc tcccacaatg gatagtcagg aaattccaca aaatcggtaa tgagataaaa
     3241 caccagccca tgagggttat acgtcaataa cagttcttca actgcctgta ccgccttttt
     3301 gtcacgcaga cgaatttcaa aacggttgat aatatcggta tgtttctttt tcgttgcctg
     3361 ttccttttgt ttttcataca ggcagaaata ttttgtgctt gcctttgagc cgatataaag
     3421 ggtacttgct aaattacggt tttttccgct taattttcca ccttgtacat tttcatagtt
     3481 tttacagcga cattccgcac caccatttct gtatttttca gatagcacag gaatatccag
     3541 cagaccgcac atatcattga ttgccaagtc aaaacgtcgg ataacaccgc cacagtcaag
     3601 acagcggttt aagaatgaat accagtccct gttctgtgcc tgtaagacat attccatgtt
     3661 gcgtgagcct gcccctttta attctaaaaa tacgcccatg tgttcatcat cagaagccat
     3721 gaccttgatt tccccgtaag cataatgctc cttatagcca taatacccat agtcataatg
     3781 gataaaataa tctgctttca tgccaagcac ctttcggata acttccaatg cgtcagtcgt
     3841 tggaaaacgg acagacagat aatcaacgag cagataaaaa ggcttatcgc agatatagta
     3901 attcagacaa cgcaggataa tgttctgtgt ttccttgctg gcgttagctt ttccgttctc
     3961 aaagcggttg atactatatc ttgatacatg ggtaagactt gcaagttctc cttgtgaaag
     4021 accgctggca agacgtttgt tcttcatttc aagtgcaaat tctaaattgt tcattgtatt
     4081 cctccaatcc cacaatagcc ttgaaatacc tgtaaaccac ctttcaaaaa gtgtgatatg
     4141 tactatcttc ttaaaacctc tcattactac tcacaggttt gggggtgtat ggtttttgac
     4201 ttgtcttttg tgaaatagtg cccccctgtt agataacggg ggcacagaca aggctcgcag
     4261 gctcgccagc cgacaaagca tttcttcccc ttgacacaag gtgtcatggc ggtaagcctg
     4321 agaggcttcc gccaccgtgt cagaaatact ttgtcagcaa gttctgtttt gccttttcta
     4381 acaagttcca tttcaaccga tattcatatc tacggattta atcagatagg tatttctttt
     4441 tgaatgtttt tatatttcat tctattgtgc ttattcgatt acaagaacat agtaattctt
     4501 atatgcttat ttgtcaatat ctttttttgc ctttacaaat catattaagc atgatagact
     4561 tataatagcg aagatataac gagatatgga gggttatgca atgaaaataa gccatttagg
     4621 aaataatata cagaccataa gaaaatttag gggaatgaaa caacaggaac ttgcggacaa
     4681 aatcggtatc aatatgcaga gcctttccaa gattgaaaga gggttgaact atcctgccta
     4741 tgaaacgctg gaaaagataa tggaagtgct ggacgttacg cccaatgaat tgttgtcggg
     4801 agaatggaag tatgtcaatc aatctgaaaa ggaagtctgt cagtttttaa gagccgaaga
     4861 acgtctaaat gcagaactga aacatggaca ctatgataac ttctttgaca gcgaggaaga
     4921 atggctggaa tatgagttag aaaagttacg ggaatatatt acagactata tcaacgggaa
     4981 aagcattgaa gcgtccgacc tttacccaat caaagaattt atccagcatt tgaagtttca
     5041 aaaattatta gaccgctatg atgatttata cagtatggat atgtttgggg aaagcataga
     5101 gggacataag tataagacac cttatcaagt cgtgaaaatg ataaacccga actcaaaaga
     5161 agatatggaa ctgttacgag aagtattaag gaataacaat tttgatgatg aggacgagta
     5221 acttttttcc tcttgcgaga agatacagca aacaaaaagc ccgatcaaga gtgcaaagca
     5281 ccaccaatgg tggcaaagct cttgacaggg ctttttcttt tctgttgtgg gcaatcaaga
     5341 ggaagaaagg gataaaatct gatttgtggt aaatcaggca aaggcaacgc aggtatcaca
     5401 gtatgaattg acaccgcata aggaaagcaa ctggaagatt gtagaataaa tgaaaaaggc
     5461 ttgcattctg catttttata aaattgcaag tctttatatt gacaagcata aaatactaag
     5521 atatacttta atcagaccaa cggtttgtat tggaggtaag ttatggcacg aaataaatat
     5581 ccagaagaaa cagtaaaact cattttagat gtttctacaa aaatgttttg tgaaaaagga
     5641 tatgataaca catcattgca agatattatc aatgaaacaa aactttctaa aggtgctatt
     5701 tatcatcatt tcgattcaaa ggaagatatt ttaaaagcaa tttttcaaag aattggaaat
     5761 gaaaatgcag taatatttgc taaaattaga gataacgata gtttaaacgg tttagaaaag
     5821 ttaagagaaa tatttaagac agcggtattc aattctaacc aaagcgtttt attgacagtt
     5881 tctcctcaac tattaaacaa tcctcgtttt cttgctatgc aaatagaaca aatgtatgaa
     5941 attgtagtac ctgaatttgt tgaacccgtt ttagaacaag gtatcaagga taagtctttg
     6001 gagattgaaa attcacatga aattgcagaa gttatcatgg tattaagtaa tgtatggctc
     6061 aacccattag ttgaaatgac ggataaagag ggtatggaaa aacgttgcaa tacattcaat
     6121 gcccttttaa atggaatagg catagacaca ttattagacg aagaaacaat ttctgcattt
     6181 atacagtttt gtaaataact gtatttgtta tccctatacg atagactgct ctataaaaga
     6241 gcagtttatt taaaccatta tataaaccga cggtctgttt gattaaataa aaaggagaga
     6301 ttttatgcaa gcagaaaaaa aactttttaa tcgtgatttt atacttgttg ttataggaca
     6361 gataatttct ttatttggaa acgctatcct acgatttgca ttaccgtact atttgttaaa
     6421 agaaactggt tcatcagctt tgtttggtat tgctacagcc tgctcttttt tacctatggt
     6481 tattttgtcg ctggtaggtg gtattattgc tgacaggaca aataaaagaa acattatggt
     6541 catactagat tttgtaacag caatcataat tttagtattt tatttgtcat tgggaaaaat
     6601 tcctatcata cctttgttta ttgttgcttt gatgttactt tatggtattt ctggtgcata
     6661 tcaaccagcg gtacaagcaa gtattcctct attggtatca tcagataaac ttaccgtcgg
     6721 taatgctata ataaatcaag taaatacttt agcaaacttt atcggaccag ctataagcgg
     6781 agtagttttt caattttatg gtatcaatcc cattttaatt gttagtatta tatgttttac
     6841 tttttcagcc attatggaaa tttttatcca tattccacac caaaaaagac ctcgtgaagt
     6901 aggtgtaatt gcagttgcta aaaacgattt aaaagatagt tatacctttt tgaaaaagga
     6961 gaaacccatt tttatatccg taatcttttt ggtttgcatt ttcaatttgg ttttaagtgc
     7021 tgttttaatt gttggtctgc ctatcttaat ttctgaagtt ttaaaaatgc cagatacact
     7081 atatggtttt tcacaatcag cattatcatt aggtggttta tgtggtggta ttttaacagc
     7141 tcttgttgct tcaaagttaa aactaaaaaa cctttatacc ttgttactta tttgttctgt
     7201 ttctgtttta cttatgggaa tttcgctatt tttagaattg cctgcaatag tttcctactg
     7261 ggtagttaca gtgatgtgct ttgtggctat gggtgtatct actatgtttg ttgttcaaat
     7321 ttacactctt gttcagacac aaacaccacc gcaattagta ggaaagataa tggctacgct
     7381 ggtagcagtt gctatgtgcg gtcaacctat tggacaaatg gtttacggta ttttattcga
     7441 tgtatttata caagaaacat gggttgttat gtttgtagct tctattgctt caatcatgat
     7501 ttcaatatat tcaaaaagaa tttttcaaaa gttagagata taatacaagg ggttaaatag
     7561 tatgt
//
LOCUS       DAZAFF010000273         6829 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.guided.123, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000273 DAZAFF010000000
VERSION     DAZAFF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.guided.123"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(284..520)
                     /locus_tag="ACG9BB_003083"
     CDS             complement(284..520)
                     /locus_tag="ACG9BB_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFN2838920.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(703..1074)
                     /locus_tag="ACG9BB_003084"
     CDS             complement(703..1074)
                     /locus_tag="ACG9BB_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFN2838921.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(1071..2630)
                     /locus_tag="ACG9BB_003085"
     CDS             complement(1071..2630)
                     /locus_tag="ACG9BB_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFN2838922.1"
                     /translation="MIEQKMALLHTKNFSAYARKMLIDGYIVNMDTSDIRAQTAEIQK
                     IGVNVNQIAKRLNGMGPVYAQGHRGYKGGAGADMAVTKIHPIKSTLKKALDYIENPDK
                     TDGKLLVASFACSYETADIEFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGK
                     QLAEEVLQGKYPYVLTTHIDKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRL
                     CKENGLSVVKPGQSKGKSYAEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQA
                     QGYEIKPGKFISFRAPGQERFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLR
                     IDLENNIKAQQSAGYERWAKLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDE
                     AAATLKAMEHRLADMAVLIKNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAA
                     AKALKGQGVKKLPDLYALKAEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILR
                     MTPGKEWTQEL"
     gene            complement(2948..3318)
                     /locus_tag="ACG9BB_003086"
                     /pseudo
     CDS             complement(2948..3318)
                     /locus_tag="ACG9BB_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3463..3648
                     /locus_tag="ACG9BB_003087"
     CDS             3463..3648
                     /locus_tag="ACG9BB_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFN2838923.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            3806..3973
                     /locus_tag="ACG9BB_003088"
     CDS             3806..3973
                     /locus_tag="ACG9BB_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838924.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            4205..4378
                     /locus_tag="ACG9BB_003089"
     CDS             4205..4378
                     /locus_tag="ACG9BB_003089"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838925.1"
                     /translation="MKSHTARCIRKKEAAHFAEAWGVPNKQNENAAGGQVVDQNIRKG
                     YSDPLLASIIGMF"
     gene            4591..5250
                     /gene="vanR-B"
                     /locus_tag="ACG9BB_003090"
     CDS             4591..5250
                     /gene="vanR-B"
                     /locus_tag="ACG9BB_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFN2838926.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            5253..6614
                     /gene="vanS-B"
                     /locus_tag="ACG9BB_003091"
     CDS             5253..6614
                     /gene="vanS-B"
                     /locus_tag="ACG9BB_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFN2838927.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(6705..>6829)
                     /locus_tag="ACG9BB_003092"
     CDS             complement(6705..>6829)
                     /locus_tag="ACG9BB_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFN2838928.1"
                     /translation="NLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         1694 a         1599 c         1699 g         1837 t
ORIGIN      
        1 ggcgcggcgg ccatgtggtc gcttgcgtcc ggcgtctttt ctttttgcct attcctacgc
       61 atttagaacc cggtattgtc ggggatagga tgaagtattc gcttgcccct gcaaacatgc
      121 gggagaagcc ttgcagcggg cggttttcaa tcccttaaat gcgtaggttg cagctataat
      181 tagacccctg aaacagatat agccgcgcag cgggtgtatc tgtttcaggg gtgtgcaggt
      241 gatagccgca aagcggcgta agagggtgca gccctcttgc cgtctatctc tgttccagcg
      301 ggaagctcgg caatgcttcc agcagtttca gggaaatgcg ctgccgtata tcttcgtcaa
      361 tgactttctt ctttttgccg tttgcctgtc tgatctccac cgtggaaagc tcgttgatgt
      421 agtcagcata atgggccaac actttttccg tggcccacct ctcaccggca acggcggctt
      481 tgatcgtttc atagtccagc agcttttctt gtccgtccat cctgataccc ccgtcagact
      541 gattttttct atgataccat attcggaagc atggaaccag agaaaacggc gcttttctgc
      601 tggtaaattc tacgcattta gaacagcact tcggcgggga taggataaaa cacccacata
      661 ccccgctgac catgccggaa aagccttgaa atataagggc ttttacagct ctaagtgcgt
      721 aggtttccag tatcttttcc gctggcgctc ggcctctttg cggcggcgca tccgtttgga
      781 gcaggccgcg cagtatttgg cccggttaga cttcggcgtg aacgtggccc cgcactcaca
      841 gcagcgtttc acttcgtccc ggtctttggt gatctcggca tacagggccg catccagcgg
      901 gaggacagcg gccttaaacc agcggcacag aagcgaatag gaaatgctct gtacgcaaat
      961 acattcttcg ccgtcgtcca gcaacaggca gttgccgtca caatagttgc agcactcatg
     1021 cgtcagcttc cgggccttgc gatattgagg gtaattcatt cgtggaatac tcatagctct
     1081 tgcgtccatt cctttcccgg cgtcatacgc aaaatgccgt ccacattctg cttgatgatg
     1141 ccgtattcct gcatctgctt tttcgcttcg ccgtacttct catacagccg ttccttttcc
     1201 tcggccaatc gcttgtactc ggctttcagg gcgtacagat cggggagctt tttcacaccc
     1261 tgtcccttta gggcctttgc cgcagcttca tacaaaatca acgtgctttc atgctcacgc
     1321 cggaattttg ccttatccgc tgcattgcgg tattccatag ccacgggcca gagctgcttg
     1381 taggtagaaa tattctttat cagcaccgcc atatccgcaa gccggtgttc catagctttg
     1441 agggtagcgg cggcctcgtc gctggcggcg gtgatctcgg ccactttgct ttccaaatcc
     1501 gggtaggtgt ctatcccatg ttcagtcagg aaattcaaag tctttgcggc ctgcttcaaa
     1561 ttgtggagct tcgcccacct ctcatagcct gccgactgct gggccttaat gttgttttcc
     1621 agatcaatcc gcaaagaaat ccccttgcgc tctttgggcg ctctggtgat aacgcggcct
     1681 ttgatacgct ctttgatggc ttcctcggtg taggcttccc ccaatgtctt gcagcgggta
     1741 aagcgttcct gtccgggagc gcggaaagaa ataaactttc ccggcttgat ctcatagccc
     1801 tgcgcctgca tgagccgcag gaaatcatca aaatcttttg cctgcgggat agcggcgtca
     1861 atggctgctt tgagcttcgc tttccagctc gtccctttcc gctgggcgtc ccactcggca
     1921 tagctcttgc ccttactctg gccgggcttc accacggaca atccgttttc cttgcacagc
     1981 ctgtcgctgg tgcgccggat ataggcatag ctctgtttgt tggaaatgta cttgcgctga
     2041 tttaccatat ccacggcaca gaaaataatg tgattgtgga cgtggccctt atcaatatgg
     2101 gtagtcagca catagggata tttcccttgc agcacttcct cggcaagctg cttcccgatc
     2161 tcatgggcct gttccggcgt ggcttcgccg ggggcaaagg attgtatcag gtggtggccc
     2221 agattattgc ccttttggta tgcctgcgcc agcagcattt caaattcaat gtccgctgtt
     2281 tcataagagc aggcaaagga ggccaccagc agcttgccgt cagttttgtc cgggttctcg
     2341 atgtagtcaa gggctttttt cagcgttgac ttgataggat gtatctttgt aactgccata
     2401 tctgcgccag cgcccccttt atatcctcga tgtccttgtg cgtacaccgg ccccataccg
     2461 ttcagcctct ttgcaatctg attgacgttg acgccgatct tctgtatctc ggcggtctgc
     2521 gcccggatgt cgctggtgtc catgttgacg atatagccgt caatgagcat cttccgggcg
     2581 taggctgaaa agttcttcgt atgcagcagc gccattttct gttcaatcat agcccgttcc
     2641 tccggcgtca cagggacgcg cagcacgatt tttcgctttc tgtttgccat gcttcaccgt
     2701 cctttccatt caggggtttg gggatttccc caacaagcaa aacggacgcg gcccggcagg
     2761 gacggggcag gccgtttttc cgggagtggc tacactccct atgcttgcta cgttactgtt
     2821 tctccactaa caagacgttt ccaaagcccc aaactacccg caaaatagcc ctctggaaag
     2881 aaaaaagtgc aaaaaaatag aagctacgct gtgcagctcc cgctaaaagt aaaacagagg
     2941 atgccagtta cacatcctcc gtttcattct tcttcggctt ttcaattagt atggcgctgt
     3001 caatcgcgcc ctcgatgatc ggcaggtatt cttcggggca gagtttcagc ttgtggctga
     3061 ccctttgccg cagttcgctt tcctcccgca tgatctccgg gttaaagtag cgttccacag
     3121 gaagcccgca ggctttaata agctgtatga caaccggcag actgggaatg gcccgtcgtt
     3181 ttcgatgttg gcaagatagc gccagtcaat atttattttc tctgccattg ctttgcgcga
     3241 aacgcgcttt gcttgccggg ccgctttcac atccgcgccg aaagtttcaa agccgggaca
     3301 atcttcaata ttcgccatat cgcacctccc acttacattg tatatttcat actctgcccg
     3361 tggaatgttg gtatatgcac ctttcttaaa gtttatattt cacattttgt ttcttttatt
     3421 cttcgagcaa aagaattata atgtaccctg tggaggtgta ctatggacta catgacgttg
     3481 aaagaggcag gcgagaaatg gggtgtgccc ccacgatgga taaactacta ttgtgctgcg
     3541 ggacgcatcc ccggcgctgt aaagatggcg acgatctggc tcattcccaa aactgcggaa
     3601 aagccgattg acaggcgtac aaagaaagga aaggcggtta ctgaatagta taatgaaacc
     3661 ggcgcatttt ggatatggtt ttgaagagag agaaagcatg gtattttgtc acggaaaaac
     3721 tataagatga tagatggcag gctgctccag accgataaaa agtattctca tctgaaacag
     3781 aaagaaaaga tttacacatg gatgtatgaa gaagcgaaac tttatcatga cagaaatgga
     3841 aaatgcccga aacaaagacg aggaagctgc tgctgtatca gccgtatatg gtagaattga
     3901 aaaggccggc atctggattc tttatggtga agtctccaga cattggataa ctgctggtct
     3961 aatgcgcctt tgagtatggg gtgattgatt ttaataccgg ggaggtacgg ctttttgagg
     4021 aaaatattct gggatttaca tttggcgatt attacatttc ctgtaactgc aaggacacgg
     4081 atgatgaaga tatgaggaat atagacttgc tgattttcta ctgtcttgaa cggtattagg
     4141 actgaatctg ctgcgcggag tgcggcaaga gcgaccggag ggagtacaca caggccctgc
     4201 ctgtgtgaaa agccataccg caaggtgcat tcgcaaaaaa gaggccgcgc attttgctga
     4261 ggcttggggt gtccccaaca agcaaaacga gaacgccgca ggcgggcaag tggtggatca
     4321 gaatatccgt aaaggatatt ctgatccatt gcttgccagt attatcggga tgttttgaca
     4381 accgctgtaa tcgaaacatc ccaaacatta agaaggtgtt aattattatt atcttgattt
     4441 aattcgttta aaccaataat atagatacat acaccatgaa aagaagcgag tatcccaatg
     4501 acggatgatt aaaatccgaa aagaaatcgc tttgaaacta cagggaaact acagactgtt
     4561 atgttatctt cttaaaatgg agggattttt atgtcgatac gaattctact tgtcgaggat
     4621 gatgatcata tctgtaatac agtaagggcg tttctggctg aggcaggata tcaggtggat
     4681 gcctgcacag atggaaatga ggcatacacc aagttttacg aaaacactta tcaactggtt
     4741 attcttgata ttatgctgcc cggtatgaac gggcatgaac ttttgcgtga atttcgtgcg
     4801 aaaaatgata ctcccattct gatgatgaca gccctgtcgg atgacgaaaa ccaaatccgg
     4861 gcgtttgatg cagaggcaga cgactatgta acaaagccat tcaagatgca gattttacta
     4921 aagcgggtgg aagccctgct gcggcgcagc ggtgcgctgg caaaggaaat ccgtgtcggc
     4981 aggctgacac ttctgccgga ggatttcacg gtactttgtg acggtacaga gctgcccctg
     5041 acacgaaaag aatttgaaat ccttttgctg ctggtgcaga acaaaggcag aaccttaacg
     5101 catgaaatca ttttgtcacg tatatgggga tatgactttg agggtgatgg cagcacagtc
     5161 cacactcata tcaaaaattt gcgggccaag ctgccggaaa atatcatcaa aaccattcgc
     5221 ggtgtaggat accgattgga ggaatcataa taatggaaag aaaagggatt ttcattaagg
     5281 ttttttccta tacgatcatt gtcctgctac tgcttgtcgg tgtaacagca acactgtttg
     5341 cacagcaatt tgtgtcttat ttcagagtga tggaattaca gcaaacagta aaatcctatc
     5401 agccattggt ggaactgatt cagaatagcg ataggcttga tattcaagag gtggcagggc
     5461 tgtttcacta caataaccaa tcctttgagt tttatattga agataaagag ggaagcgtac
     5521 tctatgccac accaaatgcc aatacatcaa atagttttag acccaacttt ctttatgtgg
     5581 tacatagaga tgataatatt tcgattgttg ctcaaagcaa ggcaggtgtg ggattgcttt
     5641 atcaagggct gacaattcgg ggaattgtta tgattgcgat aatggttgta ttcagccttt
     5701 tatgcgcgta tatctttgcg cggcaaatga caacgccgat caaagcctta gcggacagtg
     5761 cgaataaaat ggcaaacctg aaagatgtac cgccgccgct ggagcgaaag gatgagcttg
     5821 gcgcactggc tcatgatatg cattccatgt atgtcaggct gaaagaaacc atcgcaaggc
     5881 tggaggatga aatcgcaagg gaacatgagt tggaggaaac acagcgatat ttctttgcgg
     5941 cagcttctca tgagctaaaa acgcccatcg cggctacaag cgttctgttg gagggaatgc
     6001 ttgaaaatat cggtgactac aaagatcatt ctaagtatct gcgcgaatgc atcaaaatga
     6061 tggataggca gggcaaaatc atttccgaaa tactggagct tgtcagcctg aatgatggga
     6121 gaatcgtacc catagctgaa ccgttggaca tagggcgcac ggttgccgag ttgctgcccg
     6181 attttcaaac cttggcagag gcaaacaacc agcggttcgt cacagatatt ccagccgggc
     6241 aaattgtcct gtccgatccg aggctgctcc aaaaggcact atccaatgtc atattgaatg
     6301 cagttcagaa cacgccgcag ggaggcgagg tacggatatg gagtgagcct ggtgctgaaa
     6361 aatgccgcct ttttgttttg aacatgggcg ttcacattga tgatactgcg cttccaaggc
     6421 tgttcacccc attctatcgc attgatcagg cgcgaagcag aaaaagtggg cgaagcagaa
     6481 aaagtgggcg aagcggttta ggacttgcca tcgtacaaaa aacgctggat gccatgagcc
     6541 tccagtatgc gctggaaaac acctcggatg gcgttttgtt ctggctggat ttaccgctca
     6601 catcaacatt gtaaatattt aaaacttaaa tgattttggg ctctttgtca aataggactg
     6661 atagagtgag ataagaacat ctaaggtaga attccttttc tactttagat gttttttgat
     6721 tcataggtaa tcagattatt tagtaagaaa attctggtct tcattttccg aaagcttcga
     6781 aagccataag cgactcgttt caatgctttg atgtgggtat gcagatttt
//
LOCUS       DAZAFF010000274         5709 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.guided.139, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000274 DAZAFF010000000
VERSION     DAZAFF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.guided.139"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1217)
                     /locus_tag="ACG9BB_003093"
     CDS             complement(<1..1217)
                     /locus_tag="ACG9BB_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN2838929.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVA"
     gene            1496..2302
                     /locus_tag="ACG9BB_003094"
     CDS             1496..2302
                     /locus_tag="ACG9BB_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFN2838930.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            2320..3027
                     /locus_tag="ACG9BB_003095"
     CDS             2320..3027
                     /locus_tag="ACG9BB_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFN2838931.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            3024..3995
                     /gene="vanH"
                     /locus_tag="ACG9BB_003096"
     CDS             3024..3995
                     /gene="vanH"
                     /locus_tag="ACG9BB_003096"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH"
                     /protein_id="HFN2838932.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGARVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            3988..5016
                     /gene="vanB"
                     /locus_tag="ACG9BB_003097"
     CDS             3988..5016
                     /gene="vanB"
                     /locus_tag="ACG9BB_003097"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFN2838933.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            5022..5630
                     /locus_tag="ACG9BB_003098"
     CDS             5022..5630
                     /locus_tag="ACG9BB_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFN2838934.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         1619 a         1158 c         1452 g         1480 t
ORIGIN      
        1 gcgactcgtt tcaatgcttt gatgtgggta tgcagatttt ctattttggc atttgaataa
       61 ggcaattcga gggctaaacg aatgccggat tcatagttta taaaagcttt ttttatagaa
      121 tgcagttctt ttggaacgga attgggcatc gttcttacaa gtttaaagaa agtgtccgcc
      181 tctttgtcgt gaaacgcttg aagaatttct tgatagtaaa aataggcgat tcgtaattcg
      241 tcagagatcg aaagcagacg atccaccact tcactttcgg tgagatacgc ccaaccaaaa
      301 ttgcgccatt tggtccattt gtcataaaca agcttgtcct gtcgtttgat cagaagccga
      361 gacaacgcct ttaattgtcg ataatatttg gcttgtgttg gatcgtttcg atcaaagctt
      421 ttcatgattc gtacgcgaat atcctcaaat ttacgaatga gatgtttgac aatgtgaaaa
      481 cgatccacaa cgagttttgc ctttggaaaa cattccttta tcagagaggc ataagaaaca
      541 ttcatgtccg tcacgatgac ctttaccttc aatcgtgcgc gtcgttcaaa ttgcataaaa
      601 taagaaacga gagccttttt cttccgtgaa ggaagaatat ccagtaattt tccagtatcg
      661 ccatccatac aagaaaagga gtagggaccg acttgatttt tcagtgaacg aaattcatcc
      721 attaataaac aacgcggtaa ccaatttctt tttacttttg tcatggggat cagagaagcg
      781 agtgtccgag aaacggtcga ccaagaaacg ccgcaatgct tagcaattgt cgtcattgct
      841 tgtttttcag ttagctcttc taaaatttta tagcgcactt gtttagcgat aaagcagttt
      901 ctatcgacaa tgtaggtatt cgccgtaaaa taggtgtggc agccacgaca gtggtagcgt
      961 tgtttcgcta aagcaagata agtcagttgg ttcgcacatt tattaagttg aatgagcgat
     1021 tttttctttc cgttcttaac gatcatgcct tcatgattca tctgtttaca caaaggacag
     1081 cgacgaattt ctttacttaa cgtcgcagaa acaacaagac tcttgatatt cttaatgctc
     1141 ttcgattcca catttgtgat gaaaatgttt ttatctttga ttccaagtag tagtttgata
     1201 tgatgaagat caggcataca gaacgctcct ttgattgtgt tgtggtgatt caattctaac
     1261 ggagcttctg tatgttttgt tttttcatgc tcaaaaggga ccgatagaat gattttctat
     1321 cagtcctaga aattatagag ccgattttga ccgacaggta taaccctgcc ggtctttttt
     1381 tgctgctaca ggaaaactac agactggcta cagggaaagt acagatgcgc ttgccataat
     1441 aacaatcgta ccagccacaa atcgtagttt tattgcaaag gaggcattcg atcaaatgga
     1501 aaaaagcaac tatcattcca atgcggatca tcacaaacgg catatgaaac aatccgtgga
     1561 aaagcgggct tttctatgtg cgttcattat ttcgttcaca gtctgcacgc tgtttttggg
     1621 gtggagactg gcttccgtat tggaggcaac acagataccg cccatccctg caactcatac
     1681 aggcagcagc actgacgtag tggagaattt ggaggaaaac gctcttgcca ccgccaaaga
     1741 acagggagat gaacaggaat ggagcctgat tttagtgaac aggcagaacc ccatccccgc
     1801 acagtacgat gtggagcttg agcaactatc aaatggtgag cggatagata ttcggatttc
     1861 tccctatctt caagatttgt ttgatgccgc aagaactgat ggagtttacc cgattgtcgc
     1921 atccggatac cgaacaacag aaaaacagca agaaattatg gatgaaaaaa ttgccgaata
     1981 taaggcgaaa ggctacacct ctgcacaggc taaagcggaa gcagaaactt gggtggccgt
     2041 gccgggaacg agcgagcatc agcttggtct tgctgtggat atcaatgcgg acggaattca
     2101 ttcaacaggc aacgaggttt atagatggct ggatgaaaac agctatcgtt ttggttttat
     2161 tcgtcgctac ccaccagaca agacagagat aaccggtgtg agcaacgagc cgtggcatta
     2221 ccgatatgtt ggcatcgaag ctgccacaga gatgtacaac caaggggttt gccttgagga
     2281 atatttaaaa ccagaaaaat gagaaaagga tgttatgtta tggacagaaa aagattgaca
     2341 cagcgtttcc cgttcctgct tccaatgaga cgggcgcaga gaaaaatgtg cttttatgcg
     2401 ggaatgagat ttgacggctg tcgctatgcg cagacgatag gggaaaaatc gctttcccat
     2461 ttgctctttg aaacggattg cgcattatac aaccacaata ccggatttga catgatatac
     2521 caggaaaaca aggtgttcaa cttaaagctg gcagcaaaga ccttaaacgg cttattgata
     2581 agaccggggg aaaccttttc tttctggttg ttcctgcata cgccattgac aattatccag
     2641 cgcagaggtc acgaagtaaa ggagtttcca gagccaaaca gtgacgagat taaaggggtg
     2701 gatgcaacca tttcagaggg ctggattgat ttaaaagtgc gaaacgatac cgactgcacc
     2761 taccaaatat gggtgacctt agatgatgag aaaatcatcg gtcaggtgtt cgccgacaaa
     2821 gagccccaag cattatacaa aattacaaat ggcagtatcc agtatgtccg tgaaagtggc
     2881 gggatttatg aatatgccca ggttaaacgg atgcaagttg ccttaggtac cggggaaata
     2941 atagattgca agctgcttta tacaaacaaa tgcaaaatct gttatcccct cccggaaagt
     3001 gtggatattc aggaggagaa ccaatgagaa aaagtgtggg cattactgtt tttggatgcg
     3061 aacaggatga ggcaaatgct ttccgcgctt tatcgccgga ttttcatatt atccctacgc
     3121 tgattagcga tgcgatatcg gcagacaacg caaaattggc cgctggcaat caatgcgtta
     3181 gcgtaagcca taagtccgag gtttccgagg cgacaattct tgcgctgaga aaggtcgggg
     3241 taaaatacat ttctacccgc agcatcggct gcgatcacat tgatacgact gccgccgaga
     3301 gaatgggaat ctcggttggc acggttgcgt attcgccgga cagcgttgcg gattatgctt
     3361 tgatgctgat gctgatggcc atacggggtg caaaacccac catgcacgcc gtggcgcaac
     3421 aagatttcag attggatcgt atccggggga aagaactggg ggatatgact gtgggagtta
     3481 ttggaaccgg ccatatcggg caagcggtcg tcaaaaggct gcggggattt ggatgccatg
     3541 tgctggccta tgataacagc cgaaaaatgg atgcagatta tgtccagctt gatgagcttc
     3601 taaaaaacag cgatattgtt acgctccatg tgccgctttg tgcggatacc cgccatctga
     3661 tcggtcagaa gcaaattgga gagatgaagc aaggcgcatt tttaatcaac accgggcgcg
     3721 gagcgcgtgt cgataccggg gcgctggtgg aggcgttgga aagcggaaag ctgggtggtg
     3781 cggcactgga tgtgttggag ggcgaggatc agtttgttta taccgattgc tcgcagaaag
     3841 tgcttgacca tccctttttg tcgcagcttt taaggatgcc gaatgtgatc atcacacccc
     3901 atacggcgta ctacactgag cgtgtgctgc aggatactac agaaaaaaca atcaggaatt
     3961 gtctcaattt tgaaaggagt ttacagcatg aatagaataa aagtcgcaat catcttcggc
     4021 ggttgctcgg aggaacatga tgtgtcggta aaagccgcaa tagaaattgc tgcgaacatt
     4081 gatacggaaa aattcgatcc gcactacatc ggaattacaa aaaacggtgt atggaagcta
     4141 tgcaagaagc catgtacgga atgggaagcc gacagtctcc ccgccatact ctccccggat
     4201 aggaaaacgc atgggctgct tgtcatgaaa gaaagcgaat acgaaacacg gcgtattgat
     4261 gtggctttcc cggttttgca tggcaaatgc ggggaggatg gtgcgataca ggggctgttt
     4321 gtattgtctg gtatccccta tgtgggctgt gatattcaaa gctccgcagc ttgcatggac
     4381 aaatcactgg cctacattct tacaaaaaat gcgggcatcg ccgttcccga atttcaaatg
     4441 attgataaag gtgacaagcc ggaggcgggt gcgcttacct accctgtctt tgtgaagccg
     4501 gcacggtcag gttcgtcctt tggcgtaacc aaagtaaacg gtacggaaga acttaacgct
     4561 gcgatagaag cggcaggaca atatgatgga aaaatcttaa ttgagcaagc gatttcgggc
     4621 tgtgaggtcg ggtgtgcggt catggggaac gaggatgatt tgattgtcgg cgaagtggat
     4681 caaatccggc tgagccacgg tatcttccgc atccatcagg aaaacgagcc ggaaaaaggc
     4741 tcagaaaatg cgatgattac agttcccgca gacattccgg tcgaggaacg aaatcgggtg
     4801 caggaaacgg caaagaaagt atatcgggtg cttggatgca gagggcttgc ccgtgttgat
     4861 ctttttttgc aggaggatgg cggcatcgtt ctaaatgagg tcaataccct gcccggtttt
     4921 acatcgtaca gccgctaccc acgtatgatg gccgccgcag gaatcacgct tcctgcactg
     4981 attgacagcc tgattacatt ggcgttaaag aggtgataag tatggaaaat ggttttttgt
     5041 ttttagatga aatgttgcat ggcgtccgtt gggatgccaa gtacgccaca tgggataact
     5101 tcacaggaaa accggtggat gggtatgagg tgaatcgcat catcggcaca aaggccgtgg
     5161 cgtttgctct gcgcgaggca caaatccatg cggctgcgct tggctatggc ttgcttttat
     5221 gggatggata tcggccaaga actgcggtgg actgcttcct gcgttgggca gcgcaaccgg
     5281 aggacaatct cacaaaagaa aaattttacc ccaatataga gcgagccgag ttgattacaa
     5341 agggttatgt ggcctcacaa tccagccata gccgtggaag cgcaattgat cttacgctct
     5401 accacctgga tacaggggaa cttgtttcaa tgggaagtaa cttcgatttt atggacgaac
     5461 ggtcgcatca tacagcaaaa gggatagggg atgcagaggc acaaaatcga agatgcttgc
     5521 gtaaaatcat ggaaagcagc ggatttcagt cttatcgctt tgaatggtgg cactataagt
     5581 tgattgatga gccatacccc gatacctatt ttaattttgc cgtttcataa tgaaagtatt
     5641 ggttttgtat cagattttgg atttgataaa atctatgctg gtcatggaca atcttttact
     5701 ggaaagtgg
//
LOCUS       DAZAFF010000275         5709 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.guided.140, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000275 DAZAFF010000000
VERSION     DAZAFF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.guided.140"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1217)
                     /locus_tag="ACG9BB_003099"
     CDS             complement(<1..1217)
                     /locus_tag="ACG9BB_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN2838935.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVA"
     gene            1496..2302
                     /locus_tag="ACG9BB_003100"
     CDS             1496..2302
                     /locus_tag="ACG9BB_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFN2838936.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            2320..3027
                     /locus_tag="ACG9BB_003101"
     CDS             2320..3027
                     /locus_tag="ACG9BB_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFN2838937.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            3024..3995
                     /gene="vanH"
                     /locus_tag="ACG9BB_003102"
     CDS             3024..3995
                     /gene="vanH"
                     /locus_tag="ACG9BB_003102"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH"
                     /protein_id="HFN2838938.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALGEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            3988..5016
                     /gene="vanB"
                     /locus_tag="ACG9BB_003103"
     CDS             3988..5016
                     /gene="vanB"
                     /locus_tag="ACG9BB_003103"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFN2838939.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            5022..5630
                     /locus_tag="ACG9BB_003104"
     CDS             5022..5630
                     /locus_tag="ACG9BB_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFN2838940.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         1619 a         1158 c         1452 g         1480 t
ORIGIN      
        1 gcgactcgtt tcaatgcttt gatgtgggta tgcagatttt ctattttggc atttgaataa
       61 ggcaattcga gggctaaacg aatgccggat tcatagttta taaaagcttt ttttatagaa
      121 tgcagttctt ttggaacgga attgggcatc gttcttacaa gtttaaagaa agtgtccgcc
      181 tctttgtcgt gaaacgcttg aagaatttct tgatagtaaa aataggcgat tcgtaattcg
      241 tcagagatcg aaagcagacg atccaccact tcactttcgg tgagatacgc ccaaccaaaa
      301 ttgcgccatt tggtccattt gtcataaaca agcttgtcct gtcgtttgat cagaagccga
      361 gacaacgcct ttaattgtcg ataatatttg gcttgtgttg gatcgtttcg atcaaagctt
      421 ttcatgattc gtacgcgaat atcctcaaat ttacgaatga gatgtttgac aatgtgaaaa
      481 cgatccacaa cgagttttgc ctttggaaaa cattccttta tcagagaggc ataagaaaca
      541 ttcatgtccg tcacgatgac ctttaccttc aatcgtgcgc gtcgttcaaa ttgcataaaa
      601 taagaaacga gagccttttt cttccgtgaa ggaagaatat ccagtaattt tccagtatcg
      661 ccatccatac aagaaaagga gtagggaccg acttgatttt tcagtgaacg aaattcatcc
      721 attaataaac aacgcggtaa ccaatttctt tttacttttg tcatggggat cagagaagcg
      781 agtgtccgag aaacggtcga ccaagaaacg ccgcaatgct tagcaattgt cgtcattgct
      841 tgtttttcag ttagctcttc taaaatttta tagcgcactt gtttagcgat aaagcagttt
      901 ctatcgacaa tgtaggtatt cgccgtaaaa taggtgtggc agccacgaca gtggtagcgt
      961 tgtttcgcta aagcaagata agtcagttgg ttcgcacatt tattaagttg aatgagcgat
     1021 tttttctttc cgttcttaac gatcatgcct tcatgattca tctgtttaca caaaggacag
     1081 cgacgaattt ctttacttaa cgtcgcagaa acaacaagac tcttgatatt cttaatgctc
     1141 ttcgattcca catttgtgat gaaaatgttt ttatctttga ttccaagtag tagtttgata
     1201 tgatgaagat caggcataca gaacgctcct ttgattgtgt tgtggtgatt caattctaac
     1261 ggagcttctg tatgttttgt tttttcatgc tcaaaaggga ccgatagaat gattttctat
     1321 cagtcctaga aattatagag ccgattttga ccgacaggta taaccctgcc ggtctttttt
     1381 tgctgctaca ggaaaactac agactggcta cagggaaagt acagatgcgc ttgccataat
     1441 aacaatcgta ccagccacaa atcgtagttt tattgcaaag gaggcattcg atcaaatgga
     1501 aaaaagcaac tatcattcca atgcggatca tcacaaacgg catatgaaac aatccgtgga
     1561 aaagcgggct tttctatgtg cgttcattat ttcgttcaca gtctgcacgc tgtttttggg
     1621 gtggagactg gcttccgtat tggaggcaac acagataccg cccatccctg caactcatac
     1681 aggcagcagc actgacgtag tggagaattt ggaggaaaac gctcttgcca ccgccaaaga
     1741 acagggagat gaacaggaat ggagcctgat tttagtgaac aggcagaacc ccatccccgc
     1801 acagtacgat gtggagcttg agcaactatc aaatggtgag cggatagata ttcggatttc
     1861 tccctatctt caagatttgt ttgatgccgc aagaactgat ggagtttacc cgattgtcgc
     1921 atccggatac cgaacaacag aaaaacagca agaaattatg gatgaaaaaa ttgccgaata
     1981 taaggcgaaa ggctacacct ctgcacaggc taaagcggaa gcagaaactt gggtggccgt
     2041 gccgggaacg agcgagcatc agcttggtct tgctgtggat atcaatgcgg acggaattca
     2101 ttcaacaggc aacgaggttt atagatggct ggatgaaaac agctatcgtt ttggttttat
     2161 tcgtcgctac ccaccagaca agacagagat aaccggtgtg agcaacgagc cgtggcatta
     2221 ccgatatgtt ggcatcgaag ctgccacaga gatgtacaac caaggggttt gccttgagga
     2281 atatttaaaa ccagaaaaat gagaaaagga tgttatgtta tggacagaaa aagattgaca
     2341 cagcgtttcc cgttcctgct tccaatgaga cgggcgcaga gaaaaatgtg cttttatgcg
     2401 ggaatgagat ttgacggctg tcgctatgcg cagacgatag gggaaaaatc gctttcccat
     2461 ttgctctttg aaacggattg cgcattatac aaccacaata ccggatttga catgatatac
     2521 caggaaaaca aggtgttcaa cttaaagctg gcagcaaaga ccttaaacgg cttattgata
     2581 agaccggggg aaaccttttc tttctggttg ttcctgcata cgccattgac aattatccag
     2641 cgcagaggtc acgaagtaaa ggagtttcca gagccaaaca gtgacgagat taaaggggtg
     2701 gatgcaacca tttcagaggg ctggattgat ttaaaagtgc gaaacgatac cgactgcacc
     2761 taccaaatat gggtgacctt agatgatgag aaaatcatcg gtcaggtgtt cgccgacaaa
     2821 gagccccaag cattatacaa aattacaaat ggcagtatcc agtatgtccg tgaaagtggc
     2881 gggatttatg aatatgccca ggttaaacgg atgcaagttg ccttaggtac cggggaaata
     2941 atagattgca agctgcttta tacaaacaaa tgcaaaatct gttatcccct cccggaaagt
     3001 gtggatattc aggaggagaa ccaatgagaa aaagtgtggg cattactgtt tttggatgcg
     3061 aacaggatga ggcaaatgct ttccgcgctt tatcgccgga ttttcatatt atccctacgc
     3121 tgattagcga tgcgatatcg gcagacaacg caaaattggc cgctggcaat caatgcgtta
     3181 gcgtaagcca taagtccgag gtttccgagg cgacaattct tgcgctgaga aaggtcgggg
     3241 taaaatacat ttctacccgc agcatcggct gcgatcacat tgatacgact gccgccgaga
     3301 gaatgggaat ctcggttggc acggttgcgt attcgccgga cagcgttgcg gattatgctt
     3361 tgatgctgat gctgatggcc atacggggtg caaaacccac catgcacgcc gtggcgcaac
     3421 aagatttcag attggatcgt atccggggga aagaactggg ggatatgact gtgggagtta
     3481 ttggaaccgg ccatatcggg caagcggtcg tcaaaaggct gcggggattt ggatgccatg
     3541 tgctggccta tgataacagc cgaaaaatgg atgcagatta tgtccagctt gatgagcttc
     3601 taaaaaacag cgatattgtt acgctccatg tgccgctttg tgcggatacc cgccatctga
     3661 tcggtcagaa gcaaattgga gagatgaagc aaggcgcatt tttaatcaac accgggcgcg
     3721 gagcgcttgt cgataccggg gcgctggggg aggcgttgga aagcggaaag ctgggtggtg
     3781 cggcactgga tgtgttggag ggcgaggatc agtttgttta taccgattgc tcgcagaaag
     3841 tgcttgacca tccctttttg tcgcagcttt taaggatgcc gaatgtgatc atcacacccc
     3901 atacggcgta ctacactgag cgtgtgctgc aggatactac agaaaaaaca atcaggaatt
     3961 gtctcaattt tgaaaggagt ttacagcatg aatagaataa aagtcgcaat catcttcggc
     4021 ggttgctcgg aggaacatga tgtgtcggta aaagccgcaa tagaaattgc tgcgaacatt
     4081 gatacggaaa aattcgatcc gcactacatc ggaattacaa aaaacggtgt atggaagcta
     4141 tgcaagaagc catgtacgga atgggaagcc gacagtctcc ccgccatact ctccccggat
     4201 aggaaaacgc atgggctgct tgtcatgaaa gaaagcgaat acgaaacacg gcgtattgat
     4261 gtggctttcc cggttttgca tggcaaatgc ggggaggatg gtgcgataca ggggctgttt
     4321 gtattgtctg gtatccccta tgtgggctgt gatattcaaa gctccgcagc ttgcatggac
     4381 aaatcactgg cctacattct tacaaaaaat gcgggcatcg ccgttcccga atttcaaatg
     4441 attgataaag gtgacaagcc ggaggcgggt gcgcttacct accctgtctt tgtgaagccg
     4501 gcacggtcag gttcgtcctt tggcgtaacc aaagtaaacg gtacggaaga acttaacgct
     4561 gcgatagaag cggcaggaca atatgatgga aaaatcttaa ttgagcaagc gatttcgggc
     4621 tgtgaggtcg ggtgtgcggt catggggaac gaggatgatt tgattgtcgg cgaagtggat
     4681 caaatccggc tgagccacgg tatcttccgc atccatcagg aaaacgagcc ggaaaaaggc
     4741 tcagaaaatg cgatgattac agttcccgca gacattccgg tcgaggaacg aaatcgggtg
     4801 caggaaacgg caaagaaagt atatcgggtg cttggatgca gagggcttgc ccgtgttgat
     4861 ctttttttgc aggaggatgg cggcatcgtt ctaaatgagg tcaataccct gcccggtttt
     4921 acatcgtaca gccgctaccc acgtatgatg gccgccgcag gaatcacgct tcctgcactg
     4981 attgacagcc tgattacatt ggcgttaaag aggtgataag tatggaaaat ggttttttgt
     5041 ttttagatga aatgttgcat ggcgtccgtt gggatgccaa gtacgccaca tgggataact
     5101 tcacaggaaa accggtggat gggtatgagg tgaatcgcat catcggcaca aaggccgtgg
     5161 cgtttgctct gcgcgaggca caaatccatg cggctgcgct tggctatggc ttgcttttat
     5221 gggatggata tcggccaaga actgcggtgg actgcttcct gcgttgggca gcgcaaccgg
     5281 aggacaatct cacaaaagaa aaattttacc ccaatataga gcgagccgag ttgattacaa
     5341 agggttatgt ggcctcacaa tccagccata gccgtggaag cgcaattgat cttacgctct
     5401 accacctgga tacaggggaa cttgtttcaa tgggaagtaa cttcgatttt atggacgaac
     5461 ggtcgcatca tacagcaaaa gggatagggg atgcagaggc acaaaatcga agatgcttgc
     5521 gtaaaatcat ggaaagcagc ggatttcagt cttatcgctt tgaatggtgg cactataagt
     5581 tgattgatga gccatacccc gatacctatt ttaattttgc cgtttcataa tgaaagtatt
     5641 ggttttgtat cagattttgg atttgataaa atctatgctg gtcatggaca atcttttact
     5701 ggaaagtgg
//
LOCUS       DAZAFF010000276         5709 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.guided.141, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000276 DAZAFF010000000
VERSION     DAZAFF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.guided.141"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1217)
                     /locus_tag="ACG9BB_003105"
     CDS             complement(<1..1217)
                     /locus_tag="ACG9BB_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFN2838941.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVA"
     gene            1496..2302
                     /locus_tag="ACG9BB_003106"
     CDS             1496..2302
                     /locus_tag="ACG9BB_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFN2838942.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            2320..3027
                     /locus_tag="ACG9BB_003107"
     CDS             2320..3027
                     /locus_tag="ACG9BB_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFN2838943.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            3024..3995
                     /locus_tag="ACG9BB_003108"
     CDS             3024..3995
                     /locus_tag="ACG9BB_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFN2838944.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            3988..5016
                     /gene="vanB"
                     /locus_tag="ACG9BB_003109"
     CDS             3988..5016
                     /gene="vanB"
                     /locus_tag="ACG9BB_003109"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFN2838945.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            5022..5630
                     /locus_tag="ACG9BB_003110"
     CDS             5022..5630
                     /locus_tag="ACG9BB_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFN2838946.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT         1619 a         1158 c         1451 g         1481 t
ORIGIN      
        1 gcgactcgtt tcaatgcttt gatgtgggta tgcagatttt ctattttggc atttgaataa
       61 ggcaattcga gggctaaacg aatgccggat tcatagttta taaaagcttt ttttatagaa
      121 tgcagttctt ttggaacgga attgggcatc gttcttacaa gtttaaagaa agtgtccgcc
      181 tctttgtcgt gaaacgcttg aagaatttct tgatagtaaa aataggcgat tcgtaattcg
      241 tcagagatcg aaagcagacg atccaccact tcactttcgg tgagatacgc ccaaccaaaa
      301 ttgcgccatt tggtccattt gtcataaaca agcttgtcct gtcgtttgat cagaagccga
      361 gacaacgcct ttaattgtcg ataatatttg gcttgtgttg gatcgtttcg atcaaagctt
      421 ttcatgattc gtacgcgaat atcctcaaat ttacgaatga gatgtttgac aatgtgaaaa
      481 cgatccacaa cgagttttgc ctttggaaaa cattccttta tcagagaggc ataagaaaca
      541 ttcatgtccg tcacgatgac ctttaccttc aatcgtgcgc gtcgttcaaa ttgcataaaa
      601 taagaaacga gagccttttt cttccgtgaa ggaagaatat ccagtaattt tccagtatcg
      661 ccatccatac aagaaaagga gtagggaccg acttgatttt tcagtgaacg aaattcatcc
      721 attaataaac aacgcggtaa ccaatttctt tttacttttg tcatggggat cagagaagcg
      781 agtgtccgag aaacggtcga ccaagaaacg ccgcaatgct tagcaattgt cgtcattgct
      841 tgtttttcag ttagctcttc taaaatttta tagcgcactt gtttagcgat aaagcagttt
      901 ctatcgacaa tgtaggtatt cgccgtaaaa taggtgtggc agccacgaca gtggtagcgt
      961 tgtttcgcta aagcaagata agtcagttgg ttcgcacatt tattaagttg aatgagcgat
     1021 tttttctttc cgttcttaac gatcatgcct tcatgattca tctgtttaca caaaggacag
     1081 cgacgaattt ctttacttaa cgtcgcagaa acaacaagac tcttgatatt cttaatgctc
     1141 ttcgattcca catttgtgat gaaaatgttt ttatctttga ttccaagtag tagtttgata
     1201 tgatgaagat caggcataca gaacgctcct ttgattgtgt tgtggtgatt caattctaac
     1261 ggagcttctg tatgttttgt tttttcatgc tcaaaaggga ccgatagaat gattttctat
     1321 cagtcctaga aattatagag ccgattttga ccgacaggta taaccctgcc ggtctttttt
     1381 tgctgctaca ggaaaactac agactggcta cagggaaagt acagatgcgc ttgccataat
     1441 aacaatcgta ccagccacaa atcgtagttt tattgcaaag gaggcattcg atcaaatgga
     1501 aaaaagcaac tatcattcca atgcggatca tcacaaacgg catatgaaac aatccgtgga
     1561 aaagcgggct tttctatgtg cgttcattat ttcgttcaca gtctgcacgc tgtttttggg
     1621 gtggagactg gcttccgtat tggaggcaac acagataccg cccatccctg caactcatac
     1681 aggcagcagc actgacgtag tggagaattt ggaggaaaac gctcttgcca ccgccaaaga
     1741 acagggagat gaacaggaat ggagcctgat tttagtgaac aggcagaacc ccatccccgc
     1801 acagtacgat gtggagcttg agcaactatc aaatggtgag cggatagata ttcggatttc
     1861 tccctatctt caagatttgt ttgatgccgc aagaactgat ggagtttacc cgattgtcgc
     1921 atccggatac cgaacaacag aaaaacagca agaaattatg gatgaaaaaa ttgccgaata
     1981 taaggcgaaa ggctacacct ctgcacaggc taaagcggaa gcagaaactt gggtggccgt
     2041 gccgggaacg agcgagcatc agcttggtct tgctgtggat atcaatgcgg acggaattca
     2101 ttcaacaggc aacgaggttt atagatggct ggatgaaaac agctatcgtt ttggttttat
     2161 tcgtcgctac ccaccagaca agacagagat aaccggtgtg agcaacgagc cgtggcatta
     2221 ccgatatgtt ggcatcgaag ctgccacaga gatgtacaac caaggggttt gccttgagga
     2281 atatttaaaa ccagaaaaat gagaaaagga tgttatgtta tggacagaaa aagattgaca
     2341 cagcgtttcc cgttcctgct tccaatgaga cgggcgcaga gaaaaatgtg cttttatgcg
     2401 ggaatgagat ttgacggctg tcgctatgcg cagacgatag gggaaaaatc gctttcccat
     2461 ttgctctttg aaacggattg cgcattatac aaccacaata ccggatttga catgatatac
     2521 caggaaaaca aggtgttcaa cttaaagctg gcagcaaaga ccttaaacgg cttattgata
     2581 agaccggggg aaaccttttc tttctggttg ttcctgcata cgccattgac aattatccag
     2641 cgcagaggtc acgaagtaaa ggagtttcca gagccaaaca gtgacgagat taaaggggtg
     2701 gatgcaacca tttcagaggg ctggattgat ttaaaagtgc gaaacgatac cgactgcacc
     2761 taccaaatat gggtgacctt agatgatgag aaaatcatcg gtcaggtgtt cgccgacaaa
     2821 gagccccaag cattatacaa aattacaaat ggcagtatcc agtatgtccg tgaaagtggc
     2881 gggatttatg aatatgccca ggttaaacgg atgcaagttg ccttaggtac cggggaaata
     2941 atagattgca agctgcttta tacaaacaaa tgcaaaatct gttatcccct cccggaaagt
     3001 gtggatattc aggaggagaa ccaatgagaa aaagtgtggg cattactgtt tttggatgcg
     3061 aacaggatga ggcaaatgct ttccgcgctt tatcgccgga ttttcatatt atccctacgc
     3121 tgattagcga tgcgatatcg gcagacaacg caaaattggc cgctggcaat caatgcgtta
     3181 gcgtaagcca taagtccgag gtttccgagg cgacaattct tgcgctgaga aaggtcgggg
     3241 taaaatacat ttctacccgc agcatcggct gcgatcacat tgatacgact gccgccgaga
     3301 gaatgggaat ctcggttggc acggttgcgt attcgccgga cagcgttgcg gattatgctt
     3361 tgatgctgat gctgatggcc atacggggtg caaaacccac catgcacgcc gtggcgcaac
     3421 aagatttcag attggatcgt atccggggga aagaactggg ggatatgact gtgggagtta
     3481 ttggaaccgg ccatatcggg caagcggtcg tcaaaaggct gcggggattt ggatgccatg
     3541 tgctggccta tgataacagc cgaaaaatgg atgcagatta tgtccagctt gatgagcttc
     3601 taaaaaacag cgatattgtt acgctccatg tgccgctttg tgcggatacc cgccatctga
     3661 tcggtcagaa gcaaattgga gagatgaagc aaggcgcatt tttaatcaac accgggcgcg
     3721 gagcgcttgt cgataccggg gcgctggtgg aggcgttgga aagcggaaag ctgggtggtg
     3781 cggcactgga tgtgttggag ggcgaggatc agtttgttta taccgattgc tcgcagaaag
     3841 tgcttgacca tccctttttg tcgcagcttt taaggatgcc gaatgtgatc atcacacccc
     3901 atacggcgta ctacactgag cgtgtgctgc aggatactac agaaaaaaca atcaggaatt
     3961 gtctcaattt tgaaaggagt ttacagcatg aatagaataa aagtcgcaat catcttcggc
     4021 ggttgctcgg aggaacatga tgtgtcggta aaagccgcaa tagaaattgc tgcgaacatt
     4081 gatacggaaa aattcgatcc gcactacatc ggaattacaa aaaacggtgt atggaagcta
     4141 tgcaagaagc catgtacgga atgggaagcc gacagtctcc ccgccatact ctccccggat
     4201 aggaaaacgc atgggctgct tgtcatgaaa gaaagcgaat acgaaacacg gcgtattgat
     4261 gtggctttcc cggttttgca tggcaaatgc ggggaggatg gtgcgataca ggggctgttt
     4321 gtattgtctg gtatccccta tgtgggctgt gatattcaaa gctccgcagc ttgcatggac
     4381 aaatcactgg cctacattct tacaaaaaat gcgggcatcg ccgttcccga atttcaaatg
     4441 attgataaag gtgacaagcc ggaggcgggt gcgcttacct accctgtctt tgtgaagccg
     4501 gcacggtcag gttcgtcctt tggcgtaacc aaagtaaacg gtacggaaga acttaacgct
     4561 gcgatagaag cggcaggaca atatgatgga aaaatcttaa ttgagcaagc gatttcgggc
     4621 tgtgaggtcg ggtgtgcggt catggggaac gaggatgatt tgattgtcgg cgaagtggat
     4681 caaatccggc tgagccacgg tatcttccgc atccatcagg aaaacgagcc ggaaaaaggc
     4741 tcagaaaatg cgatgattac agttcccgca gacattccgg tcgaggaacg aaatcgggtg
     4801 caggaaacgg caaagaaagt atatcgggtg cttggatgca gagggcttgc ccgtgttgat
     4861 ctttttttgc aggaggatgg cggcatcgtt ctaaatgagg tcaataccct gcccggtttt
     4921 acatcgtaca gccgctaccc acgtatgatg gccgccgcag gaatcacgct tcctgcactg
     4981 attgacagcc tgattacatt ggcgttaaag aggtgataag tatggaaaat ggttttttgt
     5041 ttttagatga aatgttgcat ggcgtccgtt gggatgccaa gtacgccaca tgggataact
     5101 tcacaggaaa accggtggat gggtatgagg tgaatcgcat catcggcaca aaggccgtgg
     5161 cgtttgctct gcgcgaggca caaatccatg cggctgcgct tggctatggc ttgcttttat
     5221 gggatggata tcggccaaga actgcggtgg actgcttcct gcgttgggca gcgcaaccgg
     5281 aggacaatct cacaaaagaa aaattttacc ccaatataga gcgagccgag ttgattacaa
     5341 agggttatgt ggcctcacaa tccagccata gccgtggaag cgcaattgat cttacgctct
     5401 accacctgga tacaggggaa cttgtttcaa tgggaagtaa cttcgatttt atggacgaac
     5461 ggtcgcatca tacagcaaaa gggatagggg atgcagaggc acaaaatcga agatgcttgc
     5521 gtaaaatcat ggaaagcagc ggatttcagt cttatcgctt tgaatggtgg cactataagt
     5581 tgattgatga gccatacccc gatacctatt ttaattttgc cgtttcataa tgaaagtatt
     5641 ggttttgtat cagattttgg atttgataaa atctatgctg gtcatggaca atcttttact
     5701 ggaaagtgg
//
LOCUS       DAZAFF010000277         5028 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.guided.150, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000277 DAZAFF010000000
VERSION     DAZAFF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.guided.150"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1270
                     /locus_tag="ACG9BB_003111"
     CDS             <1..1270
                     /locus_tag="ACG9BB_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFN2838947.1"
                     /translation="PAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPN
                     GKISMVSFREIIEAQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSV
                     AINGIITSILMRAKPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVV
                     QEMEERYEKFAATGVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASN
                     EVEDAIIRLAQMARAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTII
                     DSNGAEKLLGRGDMLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMP
                     TDEVETTGAPEQPQDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHG
                     VVGPSEGSKPRKVFIEQEETSETLPDDNDM"
     gene            1500..2567
                     /gene="rlmN"
                     /locus_tag="ACG9BB_003112"
     CDS             1500..2567
                     /gene="rlmN"
                     /locus_tag="ACG9BB_003112"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFN2838948.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRSEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            2557..2763
                     /locus_tag="ACG9BB_003113"
     CDS             2557..2763
                     /locus_tag="ACG9BB_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838949.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(2760..3863)
                     /locus_tag="ACG9BB_003114"
     CDS             complement(2760..3863)
                     /locus_tag="ACG9BB_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFN2838950.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            4415..4963
                     /gene="aac(6')"
                     /locus_tag="ACG9BB_003115"
     CDS             4415..4963
                     /gene="aac(6')"
                     /locus_tag="ACG9BB_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFN2838951.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1657 a          868 c         1069 g         1434 t
ORIGIN      
        1 gccggctgta ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc
       61 tttagcggcg aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat
      121 tgaagtacca aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc
      181 aaaccaccca gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca
      241 aacggctgat ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa
      301 gtcagtagca attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt
      361 aaaactgatg atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca
      421 tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt
      481 acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg
      541 ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc
      601 attcatcgta gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt
      661 tgaagatgcg attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct
      721 agctactcaa cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc
      781 cagaatggca tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg
      841 ggcagaaaag ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc
      901 gatccgtgtg caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt
      961 tacagatcag caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac
     1021 gacaggtgct ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt
     1081 tgaaatgcaa acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg
     1141 agcagcacgt ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag
     1201 caagcctcgg aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa
     1261 tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg
     1321 catgatgata ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc
     1381 ttgtcttttt agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat
     1441 taaggaaatg aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt
     1501 tggaaaaaca gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa
     1561 acggcgagaa aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa
     1621 gtaatttcac agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt
     1681 ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat
     1741 atttgtttga actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg
     1801 gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa
     1861 gcgggctgct caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga
     1921 tggtccaaca ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa
     1981 tggggatcgg tgagccattt gataattacg acaatgtaat ggactttttg catatcatca
     2041 atgatgccaa aggattggca attggtgctc ggcacattac agtttccact agtggattag
     2101 cacacaaaat caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc
     2161 acgcgccgaa taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg
     2221 agaaattgat ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg
     2281 aatatatcat gttgaatcaa gtaaatgata gatcagagca tgcgcaacaa ttagcagatc
     2341 tattaaaaga taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg
     2401 aacatgatca atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga
     2461 agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt
     2521 gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc
     2581 gttttttcaa ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat
     2641 aagagaaaag attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc
     2701 aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt
     2761 taactgattt tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca
     2821 agaatggcct gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt
     2881 gttgccatgg ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg
     2941 gttaacgata ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg
     3001 aactgctgat ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg
     3061 tatccctcca ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa
     3121 ttatagttat tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg
     3181 acttgttcaa ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca
     3241 taagtcaaga gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag
     3301 cctagatcat cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca
     3361 atacctgccc ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg
     3421 tgtacaggtg gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg
     3481 acacataccg cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt
     3541 ttagctagtt tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca
     3601 aacaggacgt ctgctttttg gacactttca tgctggatca actgttcaat atttccataa
     3661 gttgaattac ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact
     3721 tttattttgt tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc
     3781 ccatattctt tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt
     3841 ggtaagaaaa cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa
     3901 ttttaaagag tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag
     3961 gataagtgat gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac
     4021 gaacaagtac aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct
     4081 accgattccc tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat
     4141 gaaaacaggt tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat
     4201 atcatgatga acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt
     4261 ttgaataaag tgtttctgta taatttttta ttttataagt aagataaata atgaaagaat
     4321 agagatactg attggtagct aaagattttt gagcgagaac atttcgtttt ttcaaaagga
     4381 ctgttagaat aagcaagaac aagggggaag acgtatgata atcagtgaat ttgaccgtaa
     4441 taatccagta ttgaaagatc agctttctga tttactgaga ctgacttggc cggaagaata
     4501 tggagacagc tcggcagaag aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc
     4561 agcggtagac caagatgagt tagtaggatt tattggtgca atccctcaat acggtatcac
     4621 aggttgggaa ttgcatccat tagttgtaga aagctcccga cgaaagaacc aaataggtac
     4681 tcgattagtc aattacttag aaaaagaagt agcttccaga ggaggaatca cgatttattt
     4741 aggtacggat gatttagacc atggaacaac gttaagtcaa acggacctgt atgagcatac
     4801 atttgataaa gtggcttcca tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa
     4861 attaggttat aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca aaccggatat
     4921 ttggatggca aaaacgatta ttcctcgacc aaattctcaa taataaaaag aggttgtgac
     4981 agaagtaatc agcttcaaga aataagaagg aatttacgaa aattactt
//
LOCUS       DAZAFF010000278         4144 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.guided.164, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000278 DAZAFF010000000
VERSION     DAZAFF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.guided.164"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..824
                     /locus_tag="ACG9BB_003116"
     CDS             132..824
                     /locus_tag="ACG9BB_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFN2838952.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1151..1648
                     /gene="dfrG"
                     /locus_tag="ACG9BB_003117"
     CDS             1151..1648
                     /gene="dfrG"
                     /locus_tag="ACG9BB_003117"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFN2838953.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1720..3672)
                     /locus_tag="ACG9BB_003118"
     CDS             complement(1720..3672)
                     /locus_tag="ACG9BB_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFN2838954.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3679..3915)
                     /locus_tag="ACG9BB_003119"
     CDS             complement(3679..3915)
                     /locus_tag="ACG9BB_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838955.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1398 a          696 c          692 g         1358 t
ORIGIN      
        1 aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata tgtaatttaa
       61 aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta ttggattaag
      121 gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca tttctatgtc
      181 taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt ggagagacag
      241 atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca gataaagagc
      301 aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg caagattcaa
      361 aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa aaaaatcaat
      421 cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca caactacaaa
      481 gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat gatgaaatca
      541 atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa ctttcagaat
      601 tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa gaaaaaacaa
      661 atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact gctgaaaact
      721 caagctggaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg gaagatacag
      781 ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc ttaacaagta
      841 aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt ttggagtaca
      901 aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac ataccaaatt
      961 acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa atttgtgatt
     1021 ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta acgggcagga
     1081 taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag ttaaaaaggc
     1141 tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag tgattggcaa
     1201 agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa atactacaaa
     1261 gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag ccttacctga
     1321 cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt gtgaaattgt
     1381 tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt ttattttcgg
     1441 aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca tcacaaaaat
     1501 acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg aatggaatga
     1561 ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact attttcatgt
     1621 atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga acagtaaaga
     1681 taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat aaattatatc
     1741 tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca aaaccttttt
     1801 atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg taatcgcaaa
     1861 ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac cacgagctgt
     1921 tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa agctattggt
     1981 cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca gagcagataa
     2041 atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg gatgtttgat
     2101 agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct cttcgtggaa
     2161 ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta gaaactttgt
     2221 tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc cgaattcttt
     2281 taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat gtgcatcgct
     2341 caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga agtcatcatc
     2401 actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat agtgagcagt
     2461 tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat tagtaatttt
     2521 ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg gttcttgtgg
     2581 ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat ggtcttcttt
     2641 gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat aggcaacatc
     2701 agaacggaac acatatcttg ataagacctc agcagataca actatatacg tttgcatatt
     2761 aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga cgttatttag
     2821 gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg tttgaagggg
     2881 ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact ccagcttatg
     2941 atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg aaacccttcc
     3001 aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat aagtagtggt
     3061 ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt gatagcgttg
     3121 agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga atggggttga
     3181 ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat cttggatgct
     3241 attgatttct ataaggcgtt taatttcttc aacaacagac caattagata aaggggttac
     3301 tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca tacctttatg
     3361 acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt ttacatcttt
     3421 gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt aattatattg
     3481 aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca ttttgttata
     3541 ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat cttttaattc
     3601 ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg ctaatctttt
     3661 aagcttggtc attgtctatc aacccactct atctttgata cccatttttt caacaatccg
     3721 cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta aaatgttcaa
     3781 taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt attgcttaac
     3841 tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct tattttttgg
     3901 ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta accattttaa
     3961 cacaccaatt tgcattccta caataattat ttcacgttcg catttttata tacattttaa
     4021 cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa aacgagtatc
     4081 aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg tactccaaaa
     4141 ctga
//
LOCUS       DAZAFF010000279         3795 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.guided.172, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000279 DAZAFF010000000
VERSION     DAZAFF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.guided.172"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..193
                     /locus_tag="ACG9BB_003120"
     CDS             110..193
                     /locus_tag="ACG9BB_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFN2838956.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="ACG9BB_003121"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="ACG9BB_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFN2838957.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="ACG9BB_003122"
     CDS             complement(1318..2181)
                     /locus_tag="ACG9BB_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFN2838958.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="ACG9BB_003123"
     CDS             complement(2183..2455)
                     /locus_tag="ACG9BB_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFN2838959.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="ACG9BB_003124"
     CDS             complement(2472..2681)
                     /locus_tag="ACG9BB_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFN2838960.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2759..3655)
                     /locus_tag="ACG9BB_003125"
     CDS             complement(2759..3655)
                     /locus_tag="ACG9BB_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFN2838961.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1174 a          662 c          586 g         1373 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattct
     2761 attctttttc attttctaat tgaatgattc gctcaatcat ttcaaaaaat acgttctcat
     2821 acatagacaa aacttttttg tcatagcctt tgtgttctgt aatgccattt ttagaccaag
     2881 tacttacttt attacttcgc ttgataatat ttcggaaaac caaattatcc tctttatgtt
     2941 ctttgtacag ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag
     3001 gaacaaaacc aatcatatct agtccagggt taaactgttc ttgcaaatca atcaaatagg
     3061 aaatatagtt ttgaatgttg tttgtacttt cttcttctgc ttgtaaaggg atcataacgt
     3121 aatcactcgc cacgattgca ttatttgtat aaacgcttgg cgttggtaca gtatcaataa
     3181 taataagatc atagtcactt tttaaaggtg ctaaaagagt agcaagcaat ctactttcat
     3241 tttcaaacgt ccatgagcga gttaattttg gcagtaacat caaatcaaac gtgccaggga
     3301 tcaagtctaa attatcagtc aaatgaataa tagaagaagc caagtttcca tttttcaagc
     3361 cttcataaaa attgacacgt ggcaattcta cctcaaaagt ttttgctaag tcttttgtca
     3421 atgttgcctg taagtcctta tcgatcatta aaacttttaa attcaatttg tctgtcaagt
     3481 aagcaaacat agtcgataat ttggactttc caacaccacc tttaaagtaa ttattcaaga
     3541 taataattgc ttcattttta ttgtttaaaa tacgtcttaa ttcttcaagt atttttagtt
     3601 tttccttctc cataactacg ccccattctt tttttgtata gtaatattgt atcattaaga
     3661 atcacaaatc acaagtgatt tgtgattaat cacttgtgat ttgtgataag tgatttgtga
     3721 ttaatatata aaagccctct ttaaagggct tttataggtt ctgttgcaaa gttttaaata
     3781 aagaataaaa tccct
//
LOCUS       DAZAFF010000280         1516 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE7383
            SAMEA115709006-rid22583623.guided.215, whole genome shotgun
            sequence.
ACCESSION   DAZAFF010000280 DAZAFF010000000
VERSION     DAZAFF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709006
            Sequence Read Archive: ERR13198724
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288637.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288637.1
            Genome Coverage       :: 139x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:04:18
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,128
            CDSs (total)                      :: 3,067
            Genes (coding)                    :: 2,977
            CDSs (with protein)               :: 2,977
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 41 of 90
            Pseudo Genes (internal stop)      :: 29 of 90
            Pseudo Genes (multiple problems)  :: 13 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709006-rid22583623.guided.215"
                     /isolate="VRE7383"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..341)
                     /locus_tag="ACG9BB_003126"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="ACG9BB_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            434..783
                     /locus_tag="ACG9BB_003127"
                     /pseudo
     CDS             434..783
                     /locus_tag="ACG9BB_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1055..1345)
                     /locus_tag="ACG9BB_003128"
     CDS             complement(1055..1345)
                     /locus_tag="ACG9BB_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFN2838962.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          486 a          295 c          209 g          526 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      961 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
     1021 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
     1081 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
     1141 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
     1201 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
     1261 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
     1321 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
     1381 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1441 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1501 gtatagattt aagtag
//
